LOCUS       DAOQFT010000001        13068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_2203_length_13068_cov_7.721970, whole genome shotgun sequence.
ACCESSION   DAOQFT010000001 DAOQFT010000000
VERSION     DAOQFT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_13068_cov_7.721970"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLR93_00005"
     CDS             complement(<1..525)
                     /locus_tag="PLR93_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger Ran-binding domain-containing
                     protein"
                     /protein_id="HNH25366.1"
                     /translation="MAEMITCPVCGTQNPANQAVCQRCQTPLNNALFQPGQSPVHKDT
                     GELEPLLPQWLKDARQSAKEEGETQQQTQQPPKPEAPAKPAASSTDFLAGLQSQAGDD
                     DDEEIPDWLASITGTSAPKQKPNEVVEEPSGTRWVETGNKSDFQQAEPEAEEEVPSWL
                     TGMQQQPNPDEKDEL"
     gene            complement(525..1139)
                     /locus_tag="PLR93_00010"
     CDS             complement(525..1139)
                     /locus_tag="PLR93_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25367.1"
                     /translation="MRIITAIIAIVVGILVLLGYFLPQAAIFQVILLDWAIILAGAAT
                     LAGVLNLVTVHSEKIRTRSKDNIYSAILILGLFATFVFSLLLKPQNIYVQTFILNGII
                     VPIEASLMALLAVSLLYAAVRLLRRRADLMGIVFVLTAALLFFGSATLPFGDVPIMST
                     LVRPWVSQILALGGARGILIGVALGTLTTGLRVLFGIDRPYGGK"
     gene            complement(1139..1870)
                     /locus_tag="PLR93_00015"
     CDS             complement(1139..1870)
                     /locus_tag="PLR93_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25368.1"
                     /translation="MTALAILGVAAFLLLSLTLWKRRSPSYLREIPAFTKLAHSLGLS
                     IEDGKRLHISLGHGGLLDAHGGSAFAGLALLRNIAERTSVSDMPAVASAGDPTLGLLS
                     QDTLQAGYQAAGVEDAYVHTTGRVTGLSPFSYAAGAMQIPQNEKVSTNIVIGHLGAEA
                     GLITDAAERNNIKLIGSSDNLVGQSILFANTQDPLIGEELFAAGAYLNEDPAHAASLT
                     VQDIFRWLIILILLGGAALKFLGTL"
     gene            complement(1867..2112)
                     /locus_tag="PLR93_00020"
     CDS             complement(1867..2112)
                     /locus_tag="PLR93_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25369.1"
                     /translation="MKSTREFWQRWAESLRRYQLHDLVASFLEAGSPLALLGAQAIYF
                     GGGLVKNDQLTALAGLLEDENETRAFVAFLSHNGASS"
     gene            complement(2236..3486)
                     /locus_tag="PLR93_00025"
     CDS             complement(2236..3486)
                     /locus_tag="PLR93_00025"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNH25370.1"
                     /translation="MPSYNEVLAVILGGGRGARLYPLTQMRSKPAVPIAGKYRLIDIP
                     ISNCINSEIFRIAILTQFNSVSLHRHITRTYNFDSFHQGWVQIWAAEQTIESADWYQG
                     TADAVRKQMLEIQATGAKYVLILAGDHLYRMDYKAMAEFHWANNADITVAVQPVAKDE
                     ANRFGVLKRESDGTISDFYEKPKDPDVQKRFVSRDDPERPFLGSMGIYMFNTKVLLDF
                     ISNFPDYDDFGGDIIPQAIKTHKVFGFDFDGYWQDIGTIRSFYETNLMLTKSNTPFDF
                     YDAKLPIYTDTRYLPSSIVEDSQMKDVLIAEGSRVLKSEITHSIIGIRSQIAGGCVIK
                     DSIVMGSDYYEREHRAGTPLGIGPNCHIEGAILDKNVHIGENVTIRPFPRNTDIDSEK
                     WYVRDGIVIIPKDVEIPSGTVIAP"
     gene            complement(3848..3930)
                     /locus_tag="PLR93_00030"
     tRNA            complement(3848..3930)
                     /locus_tag="PLR93_00030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3894..3896),aa:Leu,seq:caa)
     gene            3997..5055
                     /gene="ychF"
                     /locus_tag="PLR93_00035"
     CDS             3997..5055
                     /gene="ychF"
                     /locus_tag="PLR93_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNH25371.1"
                     /translation="MKLGIIGLPQSGKTTIFNALTRGNAPTNASAGRIEVHQSVVDVP
                     DPRVDLLSKMFNPRKTIYTKITYADIAGLDSGSAKSGISGQLLNHLNQMDALILVVRA
                     FENDNVAHPNGSINPLRDVDTMTSELLLNDLIAVERKLERLTDERKKGGTDKTLNARQ
                     TELFEKMHKALSDNKPLRDLEYTPEEEKELSSFGLLTRKRLLTVFNLGEGQSAPDAKL
                     DHPSVALMGKLEMEIAQLSAEDAAVFMDEYGIKELSLNRMINLSYELLQELTFFTVGE
                     DEVRAWVTHRGATAQEAAGEIHTDLSRGFVRAEVVAYEDLISLGSMNEAKAKGKFRLE
                     GKEYIVKEGDIMHVRSSL"
     gene            5152..7032
                     /locus_tag="PLR93_00040"
     CDS             5152..7032
                     /locus_tag="PLR93_00040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNH25372.1"
                     /translation="MAYKLSKWDLSQLYSGYDSPELENAFDMIEEQVASFEGVRSKLT
                     PDISAEQFMQVMKADELSTRVANKLYSFAGLSFTADTQDQKAQALQARVQQFLAENQN
                     RILFFSLWWKELDDANAKRLMDASGDYRYYLESMRNFKPHTLSEAEEKIINLKNVNGV
                     NALGNLYDSITNRYVFKMKVGGKEKEMTAAELFSYRYSTDPAVRAASYQAQFKVYAED
                     GPILGQIYQTILRDWHSENIGLRKFKNSIAPRNLSNDVPDEAVDALLNVARKNTGIFQ
                     RYFTMKAKHLGMKKIRRYDIYAPLAASKKTYDWDTAVHMVLDAFNNFSPKVAELAQRV
                     FDQNRIDSEIRKGKRGGAFCWGFLPEETPYVLVNYQGNSREVATLAHELGHAIHAMLA
                     EQHSVYTFHSSLPLAETASTFAEMVLIDKLLTEETDESVRRDILFKQMDDAYATVLRQ
                     SYFALFEKTAHEMTQKNASIDDLSAAYLENLKEQFGDSLELSDEFKWEWVGIPHIYQT
                     PFYVYAYAFGQLLVYALYQQFKQEGESFKPKYLKILSAGGSEAPERILSEAGVNIRDP
                     KFWQGGFDFLERLVSELEKLPVEKAKKPAAKKVTKKAVKKVAAKKPAKKAPVKKVAKK
                     KK"
     gene            complement(7073..9586)
                     /locus_tag="PLR93_00045"
     CDS             complement(7073..9586)
                     /locus_tag="PLR93_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNH25373.1"
                     /translation="MTSTLPVLRARRERRLEKQKRNENRSRGAILSVGLILSLVFGGL
                     IILGAFAYSDVTRDLPSPQILRRLLNPPDGLLLQPTRVYDRTGLQLLATFAPSESPRR
                     YIPISEQNAQHVPAEMVKALIAVADPSFETHSGYSLEGFNNYDMHPTIAQKLVSALML
                     YDEPPSLKRAIRERLLAVQITSQFGRTQIMEWYLNSADFGNYAYGIDAAAQLYFGKPA
                     DELTLAESAILAATSETPALNPHDAPQVAIQRGRELLYVMSDLGLVTAEQVDPALAET
                     PTFTEAPQTPAEVAPAFLNLLLSQVDSQIPRDRIERGGITVISTLDYDLQKNAACATE
                     LYAVRLANLPETETNCAAARLLPSLPPDAIYTDSSASAVILDPTTGQVLAMVGETNQG
                     VETPLVSAHPAGSAYDAFIYLTGFTRGLSPATLTWDIPFQDVNPNFDGKYHGAMRLRI
                     ALANGYQVPVETLKDQMGMENITKIASSFGVDVNNEAAMLDLASAYGVFGMQGVKLGQ
                     YVNDKFTPITVLRVESIDRGVMLDWSLPKSQTVVTPAMAYLMTHVLSDDPARWQTWGR
                     QNVLEIGRPAGVKPGQTADGKNTWVVGYTPYVSVAVWTGVRGENEFITPRFPAALWSA
                     LIQAASENKPVDGWSTPQGITPMTVCDPSGMLPTRECPNLVSEVFLNGSEPTQADTMF
                     REFSVNRETGLLATVFTPPELVDKRVYMIIPDEARAWAQSEGIAVPPSSYDAIQAPPV
                     KPYANITAPQLFADVNGKVLILGAATGDDFLYYRVQVGKGLNPQEWIQLGEDNYTPVE
                     GGILAEWDTTGLSGLYAVQLIVVRQDQKVDTAVIQVTVK"
     gene            9717..11381
                     /locus_tag="PLR93_00050"
     CDS             9717..11381
                     /locus_tag="PLR93_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNH25374.1"
                     /translation="MTPNIFMSYSRRETGFVDDLTHRLEKAGFNVWLDYRRLIPGTPW
                     AGQIQKGLQEAEVVLLVVSKDSIASQYVELEWRHVLNEKNKRIILVIFEAVTLPDELK
                     KYEWVDFRGSYEAGIKELISQLNQPIQETQAPPESGFKAPFIVWVSILLSAIAGVYSL
                     LSFWSVLLPLILIPLAWQVYKRNYNFSRVQTALWMSPLLNLLGLLLFSELGYFNAEGE
                     NFAVYYFRINPLLGVLVNILQWLPYSAFVLIFLFRSASMQRWGKPEANMPKFANPYRP
                     KNPNPRPVKFFIDHAEQDSVIAKDISNKLIQYGHLQEADIKSAESVLVLISRFNTDTT
                     ADPEHQIVFPVLVQTAQPSEKLSHVQWIDYRKGLKNLDALAQLLPDPTLLLTALGTRP
                     TNGTQIVMPNIFSRVVNFLTFILLLNVSTFFIYILELWANGLGYIQQYEPAFIFSNLA
                     QTALAVLVACGLGFFIIKSVTERRGLFASIPPFFAGLFGLFLILMYQYTLADYMTTLF
                     EDYGETEIYGFITIPYLFLIFGGIVLLIMIFFNWKELRRWFPKKGK"
     gene            11385..13064
                     /locus_tag="PLR93_00055"
     CDS             11385..13064
                     /locus_tag="PLR93_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNH25375.1"
                     /translation="MNLYPMMPNTFISYSRQCIGFVDDLAHKLQKQGFSIWTDYLSLV
                     PGRPWAEQIEKGLQEAELLLLVVSPESVSSRSVEIEWRHFLEHKKRIILLIFQAVPLP
                     PELAGLEWVDFRGAFAPAMKELLKRIEAPSPQRGLAPQSGFKAPFVVWVAAGLSVINA
                     VYSLFAFWTLLIPWVLVPLPARIFKREFNLSQVQTALWTQPIALFFTLGLFLELKLIN
                     IDVDFDYSNYFSLYNMVLMSLFIQIYLIPFFSILLLIVLRLPGMQRWGRPEASAPKFA
                     NIYRPNIVKPDPVHYFIDYAPEDKAIARELEKELTKVGHTPANGLASANEALVLLSRF
                     KTDTEADPEKQVVYPILIQRVKLPEKLSKVQWIDFRKGVRNLEAIAKLLPQPAKLLTA
                     LGVRPTSSGQAAMPNIVIALVDFLIIMGMVNLGSFFLYMLELTSLNLRLVAEYESFRL
                     LWMIFMQLLSTVASGWLIYFMVRSLSDRIGWFTKPLALSLGFIVILLLFFWQLILGSN
                     INDLLLHYGIVAETLFYALPFMFMFTGLCIVIVLVLFRFKALRMWLPAKGK"
BASE COUNT         3405 a         3223 c         3151 g         3289 t
ORIGIN      
        1 cagttcgtct ttttcatcag ggttgggctg ttgctgcatg cctgtcaacc agcttggcac
       61 ttcttcttcc gcctctggtt cagcttgctg gaaatcactt ttattgccag tttccaccca
      121 gcgagttcca ctaggttctt ctactacttc attgggcttt tgtttgggtg ctgacgtacc
      181 tgtaatgcta gcaagccaat cggggatctc ttcatcgtcg tcgtcccctg cttgagattg
      241 cagacctgct aaaaagtcgg tacttgaggc ggctggtttg gcaggcgctt ctggcttggg
      301 cggttgttga gtctgctgtt gagtctcgcc ttcttcttta gctgattgtc tcgcatcttt
      361 caaccattgc gggagaagtg gctctagctc accagtatct ttgtgaactg gagattgccc
      421 cggttggaat aaagcattat taagcggcgt ttgacagcgt tggcaaactg cttggttggc
      481 ggggttttgc gtaccacaga cagggcaggt aatcatttca gccattactt acccccgtat
      541 ggacggtcta tgccaaacaa gacacgcaga cctgttgtaa gtgtgccaag tgctacaccg
      601 atcaaaatac cgcgtgcacc acccaatgcc aaaatctgag agacccaagg gcgcaccaag
      661 gtactcataa tgggaacatc accgaaaggc aatgtggcag aaccaaagaa aagcaaagct
      721 gctgttagaa caaaaacaat acccatcaag tccgctcggc ggcgtaataa gcggacagca
      781 gcatataaca atgaaactgc cagcaacgcc ataagagagg cttctatcgg aacaatgatg
      841 ccattcaaaa taaaggtttg cacataaata ttttgcggct tgagcaacaa agaaaataca
      901 aatgtagcaa ataaacccaa gatcaaaata gcactataga tattgtcttt tgaacgagtg
      961 cgaatctttt ctgagtgaac tgtaacgaga ttaagtaccc ctgccaaggt tgctgcccca
     1021 gccagaataa ttgcccaatc gagcaagatg acttgaaaga tcgcggcttg cggcaggaaa
     1081 tatcccagca agacgaggat accgacaaca atggcgataa tggcagtgat aatgcgcatt
     1141 agagcgtccc taagaattta agtgctgccc cacccaaaag gatgaggatg atcagccagc
     1201 ggaaaatatc ttggacagtg aggctggcag catgggcagg gtcttcattc agataggcgc
     1261 ctgcagcaaa gagttcttca ccgatcagtg gatcttgggt attggcaaaa agaatggact
     1321 gccccaccag attatcagac gagccaatca gtttgatgtt gttgcgttcg gcagcatctg
     1381 taataagccc ggcttctgct cccaagtgtc caataacaat atttgtagaa actttctcgt
     1441 tttgaggaat ttgcattgca ccagccgcat aactaaatgg tgatagaccc gtaacacgcc
     1501 cagtggtgtg gacgtaggca tcttcgacgc cggcggcttg gtatccggct tgtaaggtgt
     1561 cttgggataa cagaccaagt gtcgggtccc ctgccgaggc gacagcaggc atatcactca
     1621 cagaggtgcg ctcagcaata ttccgcagta gcgcaagccc ggcgaaggca gatccgccgt
     1681 gtgcatcgag cagtccacca tgcccaagcg aaatgtgaag acgttttccg tcttcgatgc
     1741 ttaagccaag agaatgagca agctttgtaa aagcgggaat ttcacgtaaa tatgagggag
     1801 agcgtcgctt ccataatgta aggctaagta ataagaaagc tgcgacgcca agaatagcta
     1861 gtgcggtcat gaactcgctc cattgtgact caaaaaagcg acaaaggctc gtgtctcgtt
     1921 ttcatcttca agcaaaccgg caagagctgt aagttggtcg ttcttgacaa gtcccccacc
     1981 aaaataaatc gcctgagcac cgagcagggc aagcgggctt cctgcttcta gaaatgaggc
     2041 gacaagatcg tgaagttgat aacggcgcaa ggactcagcc cagcgttgcc agaattcgcg
     2101 cgttgacttc atagttaatg cgagtatagc ataattttaa ggttgcccct gctgcataag
     2161 agcgctaaat gttacagttt tgtgaatatt tcaagaaaaa acccctgaag aattcgatct
     2221 tcaggggttc attgtttatg gagcgatgac cgtacccgat ggtatctcta catcttttgg
     2281 aatgatgaca ataccatctc gcacatacca tttttcgctg tcgatatcgg tattgcgtgg
     2341 gaaggggcga atggttacat tctcgccaat gtgtacattc ttatcgagaa tggcaccttc
     2401 aatgtggcaa tttggtccaa ttcctaaggg cgtgcctgca cggtgttcac gttcatagta
     2461 atcagaaccc atcacaatgc tgtctttgat cacacagcca ccagcgatct gactgcgaat
     2521 accaatgatc gaatgcgtga tctcagattt caagacccgg ctaccttcag cgatcaatac
     2581 atctttcatc tggctatctt ccacaatgga agaaggcaga taacgcgtat cggtatagat
     2641 cggcagtttt gcatcgtaga aatcaaacgg agtattcgat ttcgtcagca tcaaattggt
     2701 ttcatagaac gagcggatcg ttccaatatc ttgccagtag ccgtcaaaat caaagccaaa
     2761 gactttatgg gtcttaatcg cttgcggaat aatatcgccg ccaaagtcat cgtaatctgg
     2821 gaagttgctg ataaagtcca acagtacttt ggtgttgaac atatagatac ccatggaacc
     2881 gagaaaaggt cgttcagggt cgtcacggct gacaaatctt ttttggacgt ctggatcttt
     2941 aggcttttcg taaaaatctg agatcgtacc gtctgattca cgcttcaaga cgccgaaacg
     3001 atttgcttcg tccttcgcaa cgggttgcac ggcgacggtg atatctgcat tattcgccca
     3061 gtgaaactca gccattgcct tgtagtccat acggtacaga tgatcccctg caaggatgag
     3121 tacatacttt gcacccgtgg cttgaatttc aagcatctgc ttgcgaacag catcggctgt
     3181 gccttgatac cagtcggcac tttctatcgt ctgttcggct gcccagatct gcacccaacc
     3241 ctgatggaaa gaatcaaaat tataggtgcg ggtgatatga cgatgcaatg agaccgagtt
     3301 gaactgggtc agaatcgcaa tacgaaatat ctctgagtta atacaattac taatgggaat
     3361 gtcaatgaga cggtattttc ccgcaatcgg gacggcaggc ttggatctca tttgtgtgag
     3421 cgggtaaagg cgtgccccgc gtccaccccc aaggatcaca gccaggactt cgttgtacga
     3481 cggcatatgg agcctcctgg aattttcgtt caggaatgtc gagcaatgct ccctatttta
     3541 cacccttcac cttacgaatg cgatgaatta cataagcaat tgggtatgcc aatatcacga
     3601 aaacatcaaa caacatgaaa aagagagaaa gtttcatgag ggactcctcc tacgggtcta
     3661 gacagcttaa tataaatacg tataaaaccc aaacttgttt ctattttcat tctacaatcc
     3721 ccaaatgacc cggtcaacct tacagagtag ggtacatata aaaatcaaac gtcctccatt
     3781 gcggaggacg ctgccagagg tggattattt gctcttaggg tcaagtaccc gtagggtata
     3841 atccgtgtgc cgaaggaggg aatcgaaccc tcattcccgg agggaacacg attttgagtc
     3901 gtgcgcgtct gcctattccg ccacttcggc ttaattgttg tggtctcgca ttagcgaggc
     3961 gaacgcaagt ataccactcc cattgataag gtcaagatga agctaggaat tattggactc
     4021 ccccaatctg gaaaaacgac gattttcaac gccctcaccc gtggcaacgc ccccaccaac
     4081 gcatctgctg gacgcatcga agtccatcag tctgtagtgg atgtacccga cccgcgtgtg
     4141 gaccttcttt ccaaaatgtt caacccacgc aagacaatct acaccaagat cacctatgcc
     4201 gatattgctg gtttggatag cggctctgcc aaaagcggta tttcgggtca gttgttgaat
     4261 cacctcaatc agatggacgc gcttattctg gtcgttcgtg cttttgagaa tgataatgtc
     4321 gcgcacccga acggaagcat caatcccttg cgcgatgtgg atacgatgac cagtgagttg
     4381 ttgttgaacg acctcattgc cgttgaacgg aaattagaac gcctcaccga tgaacgcaaa
     4441 aaaggcggca cagacaaaac tctcaatgcc cgccaaaccg agttatttga gaaaatgcac
     4501 aaggcacttt ctgataacaa accattgcgc gatttggaat acacccctga agaagaaaaa
     4561 gaactttcca gcttcggctt gctcacccgc aaacgtttgt taaccgtgtt caaccttggc
     4621 gagggacagt ctgcaccgga tgcgaaatta gatcatcctt cggtggcgtt gatgggcaaa
     4681 cttgaaatgg agatcgcaca gctctctgcg gaagatgcag ccgtgttcat ggatgaatat
     4741 ggcatcaagg agttgagcct caaccgtatg atcaacctct cgtatgagtt gttgcaagag
     4801 ttaaccttct tcaccgtggg tgaagatgaa gtgcgcgcct gggtcaccca ccgcggcgcg
     4861 acagcacagg aagccgcggg agaaattcac accgatcttt cgcgtggatt cgttcgcgcc
     4921 gaggtggtgg cttatgaaga tttgatcagc ctcgggtcga tgaatgaagc gaaggcaaag
     4981 ggcaagttcc gcctcgaagg caaagaatac atcgtcaaag aaggcgatat catgcacgtg
     5041 cgatccagtt tgtaaaatcc acaccgtaga atgggttaaa gttttttaag ttgtgataaa
     5101 acgtagggac gagggcaact cgtccctcta cgaataaaac aaggagacaa catggcgtat
     5161 aaactttcca agtgggacct ttctcaactt tattctggct acgacagccc agagcttgaa
     5221 aatgcattcg atatgatcga agaacaagtg gcatctttcg aaggcgtgcg cagcaaactt
     5281 acgcccgata tttctgccga acaatttatg caggtgatga aagccgatga gctttcaacc
     5341 cgcgttgcaa acaaacttta ttcgttcgcg ggtttatcct tcacagcaga tacacaagac
     5401 caaaaagcac aggctttaca agcccgtgtt caacaatttt tagcagagaa ccagaaccgc
     5461 attttgtttt tcagcttgtg gtggaaagaa ctggatgacg ccaacgccaa acgtttgatg
     5521 gatgcttctg gcgattatcg ctactatctc gaatccatgc gtaacttcaa gccgcatacc
     5581 ttgagcgaag cagaagaaaa gatcatcaac ttgaagaacg tcaacggggt gaatgcgttg
     5641 ggcaacctgt acgactcgat cacgaaccgc tatgtgttca agatgaaggt gggcggcaaa
     5701 gaaaaagaaa tgaccgctgc cgagttgttc tcttaccgct acagcacaga cccagcggtc
     5761 cgcgccgcaa gctaccaagc gcaatttaag gtatacgcag aggatggtcc catccttggg
     5821 caaatttacc agaccatctt gcgcgattgg cacagtgaga atattgggct gcgcaaattt
     5881 aagaactcca ttgcgccgcg caacttaagc aacgacgtcc ctgacgaagc ggtggatgcc
     5941 ttactcaatg tggcacgcaa gaatacaggt atcttccaac ggtacttcac catgaaagcc
     6001 aaacatcttg gcatgaagaa gatccgccgc tatgacatct atgctccgtt ggctgcttcc
     6061 aagaaaactt atgattggga cactgccgtt cacatggtgt tggatgcatt caataatttc
     6121 tcgcccaaag tggcagaact cgcccagcgc gtctttgacc agaatcgcat tgatagcgag
     6181 atccgcaaag gcaaacgcgg cggcgctttc tgttggggct ttttacccga agagactccc
     6241 tatgtgttgg tgaactatca gggcaatagc cgtgaagttg ccactcttgc gcacgaactg
     6301 ggtcatgcga ttcatgccat gctggcagaa cagcacagtg tctacacctt ccattcgtca
     6361 ctaccgttgg ctgaaaccgc ctcgaccttt gctgaaatgg tcttgatcga caaattgctc
     6421 acggaagaaa cagatgaatc ggttcgccga gacattctct tcaaacaaat ggatgatgcc
     6481 tatgcgaccg tgctgcgtca atcgtacttt gcgctgtttg aaaagaccgc gcatgagatg
     6541 actcaaaaga acgcctcaat cgatgacttg agcgctgcgt accttgaaaa cctcaaagaa
     6601 caatttggtg actcgctcga actgagcgac gaattcaaat gggaatgggt cggcattccg
     6661 cacatctatc agactccgtt ctacgtctat gcctatgctt tcggtcaatt gctggtgtac
     6721 gccttgtatc agcaattcaa gcaggaaggt gaatccttca agccgaagta cctcaagatc
     6781 ctttcggcgg gcggttcaga agcgccagaa cgcattttga gcgaagccgg tgtcaacatt
     6841 cgtgacccga aattctggca aggtggtttc gacttcctcg aacgtttggt gagtgagttg
     6901 gaaaaactgc cagtggaaaa agccaaaaag cctgcggcaa agaaagtcac caagaaggct
     6961 gtaaagaaag tggcagcgaa aaagcctgcc aagaaagcac ctgtaaagaa ggttgcgaag
     7021 aaaaagaagt aaataaaaag acccggtaat gagccgggtc tttttatttg aactatttca
     7081 ctgtcacttg aataacggct gtatctactt tttgatcctg ccggacaaca atcaactgaa
     7141 cggcatacag accgcttaga ccggttgtat cccattcagc taagatgccg ccttctaccg
     7201 gggtgtaatt atcttcgccc aattgaatcc actcttgcgg attcagcccc ttccccactt
     7261 gaacccgata atacaaaaag tcatctccgg tagcggcacc aagaataaga accttgccgt
     7321 tcacatccgc aaagagttgc ggtgccgtaa tattggcata cggcttgacc ggcggtgctt
     7381 gaatcgcatc ataagatgaa ggcggaacag cgatgccttc gctttgagcc cacgctcgcg
     7441 cctcatcagg gatgatcatg tacacacgct tatccacaag ttcgggcggg gtaaagaccg
     7501 tggcaagcaa gcccgtttcg cggttgacag aaaattcgcg gaacatcgta tcagcttggg
     7561 tgggttcgct gccattcaag aaaacttcag agaccaagtt cgggcattca cgggtaggca
     7621 acatgcccga agggtcgcac accgtcatcg gcgtaatgcc ttgcggcgtg ctccaaccat
     7681 ccacaggttt gttttcagat gccgcttgaa tcaacgcgct ccacaaggcg gctggaaagc
     7741 gcggtgtgat gaattcattc tcgccgcgca caccggtcca caccgcaaca gagacatagg
     7801 gtgtgtaccc aaccacccag gtgtttttac catcagcggt ctgaccaggc tttacgcctg
     7861 cgggtctgcc aatctctaaa acattttgcc tgccccaagt ttgccaacgt gccgggtcat
     7921 cgctcagcac atgggtcatc aaatatgcca tggctggcgt caccaccgtc tgcgacttgg
     7981 gcaaagacca atcgagcatc acgccgcggt ctatgctttc cacgcgtaat acggtaatcg
     8041 gcgtaaattt gtcgttcaca tattgcccca gtttgacccc ttgcatgcca aagactccgt
     8101 aggcactggc gaggtcgagc atggcggctt cattattaac atccacgcca aaagaagagg
     8161 caatcttcgt gatgttctcc atgcccattt ggtctttcaa ggtttcaacc ggcacctgat
     8221 atccattcgc aagtgcaatt cgcaaacgca tggcgccatg atatttgcca tcaaaattcg
     8281 ggttgacgtc ttgaaatggg atgtcccacg tcagtgtggc tggacttaac ccacgtgtga
     8341 agcctgtgag atagataaac gcatcatacg cagacccggc tgggtgcgca ctgaccagag
     8401 gtgtttctac gccttgattt gtctccccca ccatggcaag gacttgcccc gtggtggggt
     8461 ccaggataac ggcgctggca gatgaatctg tgtagatggc atcaggcggc agagacggga
     8521 gcaaacgtgc cgcagcgcag ttcgtttcgg tctctggcaa attcgcaagg cgcactgcat
     8581 acaattctgt tgcgcaggca gcattctttt gcaagtcata atctaatgta gagatgaccg
     8641 taatgccacc gcgctcaata cggtcgcgcg gaatttgaga gtcgacttgg gagagcaata
     8701 agtttagaaa cgctggagcc acttcggctg gagtctgagg cgcttctgtg aatgtgggcg
     8761 tctcagccaa ggctgggtcc acttgctcgg cggtgacgag tccaaggtcg ctcattacat
     8821 ataaaagctc acgtccgcgt tgaatggcaa cttgcggagc atcatgcgga ttcagggcag
     8881 gcgtttcact ggttgccgcc aagatcgcag actctgccag agttagttca tcggcaggtt
     8941 ttccgaagta cagttgagcg gcagcatcaa taccataggc ataattgcca aaatcagcgc
     9001 tgttaagata ccactccatg atctgtgtgc gcccgaactg agatgtaatc tgcactgcaa
     9061 gcaaacgctc gcgaatggcg cgcttcaaag atggcggttc gtcgtacaac atcaaagcgc
     9121 tgaccagctt ttgcgcaatg gtgggatgca tatcatagtt attaaaccct tcgagcgaat
     9181 agcccgaatg cgtttcgaaa cttgggtctg caaccgcaat gagtgccttc accatttcgg
     9241 cgggaacatg ctgggcattt tgttcgctga tgggaatgta acggcgcgga gattcactgg
     9301 gcgcaaaggt agctaacaat tgcaaaccgg ttcggtcgta gacccgtgtt ggctgtagca
     9361 aaagcccatc gggcgggttg agcaggcgtc gcaaaatctg aggcgaaggc aaatcgcggg
     9421 tgacatccga ataagcgaaa gcacccaaga taataagccc gccaaacacc agagagagga
     9481 taagccccac gctgagaatg gcgccgcgcg aacggttctc gttgcgcttt tgtttttcaa
     9541 gcctgcgctc acgccgcgca cggaggaccg gtaaagtaga agtcatacag gtagtatatt
     9601 gggattttga aaattggtaa atcgggaatt gtcagtttgg tgttttataa tgacaaggtt
     9661 tactggacaa ttaagcatgt tcgcctgcaa cgcatctcaa tttattggag actttcatga
     9721 caccaaatat ctttatgagt tattcaaggc gcgaaacagg gtttgtagat gacctaaccc
     9781 ataggctgga gaaagccggt tttaacgtct ggctcgatta ccgaaggtta attccaggca
     9841 cgccgtgggc tgggcaaatt caaaaaggct tgcaagaagc agaagtggtt ttgttggttg
     9901 tttccaaaga ttcaattgct tcacagtatg tagaattaga gtggcgacat gtattaaacg
     9961 aaaagaacaa gcgcattatt cttgtcatct ttgaagccgt gacgttaccg gatgaattga
    10021 aaaaatatga atgggtggat tttcgaggta gttatgaagc tggcatcaaa gaattaattt
    10081 cacaactcaa tcaacctatt caagaaacac aagccccgcc tgaaagcgga ttcaaagcgc
    10141 ccttcatcgt gtgggtaagc atccttttga gcgccatcgc tggcgtttat tcgctgcttt
    10201 ccttttggag cgtgttgctt ccattgattc tgattccact cgcctggcaa gtctataaac
    10261 gcaactataa cttctcacgc gtccaaactg ccttgtggat gtcaccttta ttaaacctgc
    10321 ttggcttact actctttagt gaacttggtt actttaatgc ggaaggcgaa aattttgcag
    10381 tatattattt tcgcatcaac ccattattgg gggtattggt aaacatctta cagtggctgc
    10441 cctattcggc tttcgttttg atctttcttt ttcgttcggc aagtatgcaa cgttggggca
    10501 agcccgaagc gaacatgccg aaatttgcca atccatatcg tccaaaaaat cccaatcccc
    10561 gccctgttaa attctttatc gatcatgcag aacaggattc tgttatcgcg aaggatatat
    10621 caaacaagtt gatccagtat gggcatcttc aagaagcgga catcaaatct gctgagtcag
    10681 tgttggtatt aatctcgcgc ttcaacaccg acacgaccgc tgaccccgaa catcaaatcg
    10741 ttttccccgt tttggttcag accgcacaac cttcagaaaa actttcgcat gtgcaatgga
    10801 ttgattatcg caaagggttg aaaaatttag acgcattggc acaattatta ccagacccaa
    10861 ccctattatt aactgcattg ggcacgcgtc ccaccaatgg cacacaaatt gtgatgccaa
    10921 acatcttcag tagagttgtc aacttcttaa cttttattct tttgctaaat gtcagtacgt
    10981 tttttatcta tattctggag ctatgggcaa atgggttggg atacatccaa cagtatgaac
    11041 cggctttcat ctttagcaac ctggcgcaaa cggcattggc tgtgcttgtg gcatgcggac
    11101 ttggattttt cataatcaaa tcggtaacag aaaggcgcgg actctttgca tccatcccgc
    11161 cgttttttgc agggctattt ggtctgtttc tcattctcat gtatcaatac accctggcag
    11221 attacatgac cacactcttc gaagactatg gcgaaacaga aatttatggt tttattacca
    11281 ttccatattt gtttcttatt tttggcggaa ttgttttgct gattatgatc ttcttcaatt
    11341 ggaaagagtt acgccgttgg ttccctaaaa aagggaaata aattttgaac ctctacccta
    11401 tgatgcccaa cacctttatc agttattcac ggcaatgcat tggttttgta gatgaccttg
    11461 cccacaagtt gcaaaaacaa ggcttcagca tctggacaga ttacctcagc ctggtgccgg
    11521 ggcgaccatg ggcagaacag atcgaaaagg gacttcaaga agccgagctg ctgctcttgg
    11581 tcgtctcgcc agagtctgtt tcctctcgca gcgtggagat cgaatggcgg cactttctcg
    11641 agcataaaaa gcgcatcatc ttattgatct ttcaagccgt gcctttgccg ccagagctag
    11701 ccggcttgga atgggtggac tttcgcggtg cctttgcccc agccatgaaa gaactcctga
    11761 aacggatcga ggcgcccagc ccacagcgtg gactcgctcc gcagtctggg ttcaaagccc
    11821 cgttcgtcgt ttgggtggcg gcgggtctca gcgtcatcaa tgccgtttat tccctcttcg
    11881 ctttttggac tctgttgatt ccctgggtgt tagttcctct gcccgcgcgc atcttcaaac
    11941 gcgagttcaa tttgtcgcaa gtccaaaccg cgttgtggac gcaacccatt gctttgtttt
    12001 tcacactggg cttgtttctc gaattgaagc tcatcaacat cgatgtagat tttgactaca
    12061 gcaattactt ctcgctctat aacatggtgt tgatgagtct cttcatacag atctatctca
    12121 tccccttctt ttccatcttg ctgttgatcg tcttacgctt acccggcatg caacgctggg
    12181 gcagacccga agccagcgcc ccaaagtttg ccaatatcta tcgcccgaat attgttaagc
    12241 cagaccccgt ccactacttc attgattacg ccccagaaga caaagccatt gcccgcgagc
    12301 tggaaaagga gttgaccaag gttggtcaca cccccgcgaa tggactcgca tccgcgaatg
    12361 aggcgcttgt gttactctcg cgcttcaaaa cggacaccga agcggaccct gaaaaacagg
    12421 tggtgtatcc catcctgatc caacgcgtga aactacccga gaaactctcc aaggtgcagt
    12481 ggatcgactt ccgcaaaggg gtgcgcaatc tcgaagccat cgccaagctt ctgccgcagc
    12541 ccgccaaact actcacagcc ttaggtgtgc gccccaccag cagtggacaa gccgccatgc
    12601 ccaacatcgt catcgccctt gtagactttc tcatcatcat gggcatggta aaccttggct
    12661 ccttcttcct ttacatgctc gagctgacca gcctcaacct gcgcttggtg gcagagtatg
    12721 aatcctttcg cctgctgtgg atgatcttta tgcaattgct ctccaccgtc gcttcgggct
    12781 ggttgatcta tttcatggtc cgttcgttga gtgaccgcat cgggtggttt accaaacccc
    12841 tggcattgtc gctaggcttc atcgtcattc tattgctgtt cttttggcaa ctcattcttg
    12901 gttcaaatat caatgacctt ttacttcact atggcattgt ggcagaaact ctcttttatg
    12961 ctctgccttt catgttcatg ttcacagggc tgtgcattgt gatcgtgctc gtgctcttcc
    13021 gcttcaaagc cttgcggatg tggctgccgg caaaaggaaa ataacaaa
//
LOCUS       DAOQFT010000002        12588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_2325_length_12588_cov_7.359132, whole genome shotgun sequence.
ACCESSION   DAOQFT010000002 DAOQFT010000000
VERSION     DAOQFT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_12588_cov_7.359132"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLR93_00060"
     CDS             complement(<1..408)
                     /locus_tag="PLR93_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25376.1"
                     /translation="MDQRNKSIQQDNELADFVDQLLDGKNPPASTSDEELVGLEKTLL
                     RLNDAFPPETLDEAKSKQMLARLKTRMRREEEEKAAKPSFWSRLFDLQANPQVGLLIA
                     AAVVVVFVIVSVPAVVQPSGSAVSGTASSGGSMY"
     gene            complement(411..977)
                     /locus_tag="PLR93_00065"
     CDS             complement(411..977)
                     /locus_tag="PLR93_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH25377.1"
                     /translation="MTTNYMQTNLVSLQDASDELLIQKYLNGEREAFDALYHRHLPKV
                     YKRVRYVVPPNDVEDVTQEIFIAALKSLSSFRGDSKFGTWLRTLTNHKVAEFYRKRTR
                     KQEPQLAPLSDAIAQVAGSTSQSMEDRIFLQRALLNLPENYQEILLLRFAEDLQFNEI
                     AELTNQNLEATKSLFRRAVTALRNHLEN"
     gene            1139..1894
                     /locus_tag="PLR93_00070"
     CDS             1139..1894
                     /locus_tag="PLR93_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNH25378.1"
                     /translation="MSKIISVHSFRGGTGKSNTTANIAALLAMDGARVGVVDTDIASP
                     GIHVLFNLDEADMENSLNDYLWGKCTIEQAAQDVTGHIGAEVKGQIFLIPSSIKAGEI
                     ARILREGYDVGLLNDGFRDVIEKLKLDYLLIDTHPGLNEETLLSIAISNALVIIMRPD
                     QQDYQGTAVTVDVAKKLDVPNLFILVNKAPQNLDLEEVRARVEQTYDAKVGAILPHSD
                     EMMMLASSGLFTVHYPEHVVTKGLRKLVDQLKA"
     gene            1924..2259
                     /locus_tag="PLR93_00075"
     CDS             1924..2259
                     /locus_tag="PLR93_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25379.1"
                     /translation="MSNQLFEAALKELEARQEDVRPSDVISFSEPLRSAVNFVVRLKR
                     FSLTEFHEKLPEFTREEVKRLADILIKRHLFDLSRFATEAEPYYEARLSALTRPLTRP
                     PSDIWKKID"
     gene            2324..4348
                     /gene="ligA"
                     /locus_tag="PLR93_00080"
     CDS             2324..4348
                     /gene="ligA"
                     /locus_tag="PLR93_00080"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNH25380.1"
                     /translation="MADKLLSRYEELKAQINFHNYRYHVLDAPIISDLEYDRLLNELK
                     KIEAEHPDWITPDSPTQRAGAKPSEKFEKVRHPAPILSLANAFGGDDARAWFERVKKL
                     DDRVEKAKFVVEPKIDGLSVVLHYRNGMFVQGATRGDGEVGEDITSNLRTIKAIPLKI
                     PVEGQKSKVKVPSYLVVRGEAFIPIKDFEELNKRLEEAGEKTYLNPRNTAAGSLRQLD
                     PVLTASRPITLLVYQIVYAEGGDVPTSQWEILEYLKSLGFPITDISKRFNTLEPAIAY
                     TETFDKGRDALPYEADGMVIKIDDLSLAADLGFVGKDPRGAIAFKFPAREVTTTLLGI
                     DAEVGRTGVLTPRASLEPVEIGGVVVRNATLHNFDYIEEKDIRIGDRVLVKRAGEVIP
                     YVIGPVVDARSGKEKKYKPPSKCPACGQDVEHIEGEVAWYCVNAACPAQLVRNVEHFV
                     SRGAMDIVGLGIKIVEQLIEAGLVNDVADLFTLKKKDLLELEGFAEKKAENLLGSIEQ
                     AKGQSLNRLLAALGIHGVGEVMAGDLARTYGNLSALSKTTADELQQIEGVGPNIAESI
                     VDWFARPANQKLLKKLKSAGVDPQMQKDEKKKEGAFTGLTFVVTGTLPNFSRDDAKEF
                     IENNGGKVTDSVSKKTSYLVLGENPGSKFDKAKSLGVKIIDEAELKKLAS"
     gene            4448..5659
                     /locus_tag="PLR93_00085"
     CDS             4448..5659
                     /locus_tag="PLR93_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH25381.1"
                     /translation="MDFHSIDNNSTRRIAALKHRVDTLRENNHYFYNQAVEEMLPGAK
                     VRVNGRVMGMYASYSYLGLVGHPRINEAAKRAVDKFGTGTNGVRTLAGTLTIHNELEE
                     TISNFKHAEAAITYTSGYVTNLTVISTLMGRGDYVFSDKINHASIVDGCLMSGAEFRR
                     FRHNDMEHLEGLLKNAPADVSKLVIADSVFSMDGDIIDLPKIVELSKKYNAWLMIDEA
                     HSVGVLGKTGTGIEEHFNMYGSIDIKMGTLSKTIPSVGGYVAGKKELIDYLRHGSRAY
                     IFSAALPPAQAAAANEAFKVILDEPERLDRLNANTQQFIGGLKSMGFDTLLTETAIVP
                     VLCGTDEKAMELTKRCQEQDVFVLPVVSPAVQEGMSRLRATVTAAHEPADIEHAMDVI
                     YQSGKAMGMVK"
     gene            5864..6961
                     /locus_tag="PLR93_00090"
     CDS             5864..6961
                     /locus_tag="PLR93_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH25382.1"
                     /translation="MKWQWKFGTFAGIDVYIHATFLLLIGWVGYSHWLEKNSWFDVLS
                     GIGFILALFLCVLLHEYGHALTARKYGIKTRDITLYPIGGVARLERMPEKPIEELWVA
                     LMGPAVNVVIAAILFAVLLLTGNLVPLTELGVASGSFLERVMVVNVSLVLFNLIPAFP
                     MDGGRVLRAILAMRMDYVKATQIAASIGQGLALLFGLIGLFGNASLLFIAFFIWIGAS
                     QEASAVQMKNVISGIPVGRAMLTDYKSLSPRDPLSRMAQLVLAGSQHDFPVIDDAERI
                     VGVVTRDDFLAALTQHGQNIAVSAVMRNNPPEVDSYDMVEVALTRIQESGFPTLPVTH
                     SGQLVGIITAENITEYLMIRTALRTAQVVSI"
     gene            7246..9942
                     /locus_tag="PLR93_00095"
     CDS             7246..9942
                     /locus_tag="PLR93_00095"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNH25383.1"
                     /translation="MNWHTIETNAALNELASSSETGLTSAQVEESKKKHGANELIERG
                     GRTPWQILWEQITATMVLILIAAAVVAGLLGDTKNTIAILSIVALYALLGFIQEYRAE
                     QAIAALKKMSVPNVRVIRDGKMQELSARELVPGDIIQLETGNVIPADLRLVEAVNLRI
                     QEAALTGESEAIEKHVAALSNEDLPLGDRRNMAYMGTVITQGRGLALVTATGMNTELG
                     KIADMIQAVKQEQTPLQRRLDALGKTLALIGVGIAVLIFILGIIRGDEVRHMLLTAVS
                     VAVAIVPEGLPAVVTITLALGAQRMLQRQALIRKLPAVETLGSVTVICSDKTGTLTEN
                     RMTVVVLDVAEHALDLTGDVARDGSLHATRGLGAPAQSSLSLAAIGGALCNDAKLVDE
                     GDDRFHTMGDPTEGALVVAAAKMGYWKSSLDSSFPRAAELPFDSDRKRMTTVHHLGQY
                     DPTVLSGLEIGDTRYIAFTKGGVDGLLDIASHVWADGKSVPLDDAWRSKIEASNERLA
                     RKGMRVLGVGFRLINAIPEIIQTDLEQNITLVGLFGMIDPPRNEVKDAVAIAKTAGIR
                     PIMITGDHPLTAMEIARQLGITDNGRVLTGVELEKITMDELKTMLDDVNVFARVAPEH
                     KLRIVQALQEKGHVAAMTGDGVNDAPALRKADIGVAMGITGTDVSKEASDLVLLDDNF
                     ATIVAAVKEGRTIYDNIRKFVRFSVAGNIGKVLVMLIAPFLGKPLPLLPLQLLWLNLL
                     TDGLLGLGMGVENPEKDTMKRKPYSPTEGVFSRGAGIQTIWVGALIGALALGLGSWYY
                     FTDRPEWQTMIFTSLAFMQIFQALASRSDKDSLVKIGVMSNPVLAGMALLVFVLQMAV
                     IYIPALANFFEVIPLRWHDLSIAAGTGLLVFLVMEVSKWFKK"
     gene            complement(9988..10674)
                     /locus_tag="PLR93_00100"
     CDS             complement(9988..10674)
                     /locus_tag="PLR93_00100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH25384.1"
                     /translation="MASLSGKTAIITGGSTEIASAVALKLAEAGANICLCGRQADLLA
                     MAVDRVKQAGGKGFFVESNLKSLEEAEGVVHQTTAEFGGLDILILISPFWSGGMIHSH
                     SVKTWDLVMDANLREPFLMSRAALPFLREQKHGEVMAIGSDSALGVYPQDGAFAVAMH
                     GLTTLMELIRAENSEHGIRTHILSPGVAVSAPFDVEGKPNLMPDHVADWVLWLLTRPD
                     HLRGNSPILI"
     gene            complement(10676..11563)
                     /locus_tag="PLR93_00105"
     CDS             complement(10676..11563)
                     /locus_tag="PLR93_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25385.1"
                     /translation="MTANLSSTPIKSNSPGIRLFRQILRNRSAQFGGILLILLIVTSY
                     AAPFITAKDPNKNAPSVALEAPSFEYPMGTDNLGRDNFTRFLYGGQLSLRVGLTGIII
                     GALIGILLGLISGYFGGWLDSFISWFNDVLMAFPDILLALAIIAILGPGINNAMLAIG
                     ISFIPSFMRLTRSSVFALREMTYIEAAKALGSSDTRIMLKHILPNSIRTLLVLITLGI
                     GSSILAGAALNFLGLGAEPPTAEWGAMLNAGMKYVRQAWWLTFFPGLGIFLAVLSINL
                     IGDAVSDAVAGTTIIKESK"
     gene            complement(11560..12486)
                     /locus_tag="PLR93_00110"
     CDS             complement(11560..12486)
                     /locus_tag="PLR93_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25386.1"
                     /translation="MLNYLIRRLFTSVLVLFGISVLVFSVIHLVPGDVTMAILGRQKV
                     NEEKVAELREQLGLNDPLYVQYGRYVSGALRGDLGKSIRTNQSVSASISEQLPGTFSL
                     AMSALAVALSIGAIFGLLAALRHGTWVDTIVMGLSVSGLSIPTFWMGLLLILFFSVKL
                     KLFPSISNGSSFADLFLPALTLGLPEAAVVARMVRASMLDVLNKEYITTARAKGLFER
                     SVIIKHALRNALIPVVTFVGLQMAYLLGGATIVETMFARQGIGRLAVQAIYSRDYPMV
                     QGVVLITATIYVLINTITDITYVLLNPKIRLK"
BASE COUNT         3162 a         3227 c         3222 g         2977 t
ORIGIN      
        1 atacatactg ccaccggaag atgctgttcc agagacggca gaacctgagg gttgaaccac
       61 agcaggaaca ctaacgatca caaaaacaac cactactgct gctgcgatca acaaaccgac
      121 ttgggggttg gcttgaagat caaaaagcct gctccaaaag gaaggcttgg ctgctttttc
      181 ctcttcttcc cggcgcatac gagtctttag gcgggcaagc atttgcttgg attttgcttc
      241 gtccagggtt tctggaggga aggcatcgtt caaacggaga agcgttttct cgaggccgac
      301 cagttcctcg tctgaggttg aggcgggagg gtttttccca tccaacaatt ggtcgacaaa
      361 gtccgcgagt tcgttatctt gttgtataga tttgtttctt tgatccatgg ttaattttcc
      421 aggtggttgc gaagtgcagt aacggctcga cgaaacaaag atttggtagc ttcaagattt
      481 tggttggtca attcagcaat ctcgttgaac tgtagatctt cggcaaaacg cagcagcaag
      541 atttcctgat agttctctgg caggttcagg agcgctcgtt ggaggaagat acgatcttcc
      601 atcgattggg acgtgctacc agcgacttgg gcaattgcgt ctgataatgg cgcaagttgc
      661 ggctcttgct tgcgggtgcg ctttcgatag aactctgcca ctttgtggtt ggtaagggtg
      721 cgcaaccatg tgccaaattt cgagtcacct cgaaatgagg aaagggactt taacgcggcg
      781 ataaagatct cctgcgtcac atcctctaca tcgttgggcg gaaccacata cctgacccgt
      841 ttgtagacct tggggaggtg gcggtgataa agagcatcga aagcttctcg ttctccatta
      901 aggtatttct ggatgagaag ttcgtcgctg gcgtcctgca gggatactag gttggtttgc
      961 atataatttg tcgtcatgaa gcgccaaaat gggtcgtttt cgtctttcag tttagaaagg
     1021 tactataaca taactgagaa atgaaaagta gggatttgat tttcaatata gttaggctat
     1081 aattatcaaa cttgttttca gagatttcga aagactatca tagaatcgga gacagtgcat
     1141 gtccaaaatc atttcagttc attccttccg cggcggcacg ggtaaatcga ataccacagc
     1201 aaatattgct gctttgctcg ccatggatgg tgcacgtgtt ggtgtagtgg atactgacat
     1261 tgcctcgccc ggtattcatg ttctcttcaa tttagatgaa gcggatatgg aaaactccct
     1321 caatgactat ttgtggggta agtgcaccat cgaacaagcc gctcaagacg tgactggaca
     1381 tatcggcgcc gaggttaagg gtcagatttt cctcatcccc tccagcatca aagcaggcga
     1441 aatcgcccgc attttgcgtg aaggttacga cgtgggtctg ttgaacgacg gcttccgcga
     1501 tgtgatcgaa aagttgaagc tggattatct tcttattgat acccaccccg gcttaaacga
     1561 agagaccttg ctctccattg ccatctccaa cgccttggtc atcatcatgc gcccagacca
     1621 gcaagattat cagggcacag cagtgaccgt ggatgttgcg aagaagctcg atgttcccaa
     1681 cctctttatt ctcgtcaaca aagcgcctca gaatctggac cttgaagagg tccgcgcccg
     1741 cgtggagcag acctacgacg cgaaagtggg agctattctc ccgcactcag atgaaatgat
     1801 gatgctcgcc agttctggtc tcttcaccgt tcattatcct gaacatgtgg tgaccaaagg
     1861 cttgcgcaaa ctggtggatc aactcaaagc ctagggcatt cgcctaggtg aagggaaggc
     1921 gccatgagca atcaactttt tgaagctgcg cttaaggaat tggaagcacg tcaggaagat
     1981 gttcgccctt cagacgtgat atccttctct gagcccctgc gttcggctgt aaatttcgtt
     2041 gtgcggctga agcgttttag tctgacagaa ttccatgaga aactgccaga gttcacacgg
     2101 gaagaagtaa aacggttggc agacattttg atcaagcgcc acctgtttga cctttcacgg
     2161 tttgccaccg aagcggaacc ctactacgaa gctcgcctct ctgcattgac ccgtccgctg
     2221 acccgcccgc catcagacat ttggaagaaa atcgattgat ttctcacccg cctgcataaa
     2281 acaggcgggt tttatttttc tctgcactgg gtataatcaa aaaatggcag ataaacttct
     2341 ctcacgatat gaagaactta aagcgcagat caacttccat aattatcggt atcacgtgtt
     2401 ggatgcgcct attatcagtg acctcgaata tgaccgccta ctcaacgaac taaaaaagat
     2461 cgaggcagaa caccccgatt ggatcactcc cgactccccc actcaacgtg ccggggcgaa
     2521 gccttcagaa aaatttgaga aggtgcgcca cccggcaccc atcctttcac ttgccaatgc
     2581 ctttggcggt gatgacgcac gagcctggtt tgaacgcgtc aaaaaattag atgaccgtgt
     2641 agaaaaggca aagttcgtgg ttgagccgaa gatcgatggc ttgtcggttg tgctgcatta
     2701 tcgcaatggc atgttcgtgc agggagccac ccgcggcgac ggcgaagtag gggaagatat
     2761 caccagcaac ttgcgcacta ttaaagccat tccactaaaa attccggtgg aagggcaaaa
     2821 gtccaaagtt aaggtacctt cctatcttgt cgtacgcggc gaggcattca tcccgatcaa
     2881 agactttgaa gaattaaaca aacgtctcga agaagctggc gaaaaaacct atctcaatcc
     2941 gcgcaacacg gcagctgggt cgctgcgtca gcttgacccg gtgttgactg catctcgccc
     3001 gatcacattg cttgtgtatc agatcgtgta cgccgaaggg ggtgatgtcc ccacctcgca
     3061 atgggaaatt ttggaatatc tcaaatcgct ggggtttccc atcacggata tttccaaacg
     3121 cttcaacacc ttggagccag ccatcgccta taccgaaacc tttgacaaag gacgagacgc
     3181 gcttccttac gaagccgatg gcatggtcat aaaaatcgac gatttatccc tcgccgcaga
     3241 cttgggcttt gtcggcaaag acccgcgcgg agccatcgcc ttcaagttcc cagcccgcga
     3301 agtgaccacc accctgctcg gcattgacgc cgaagtgggg cgcaccggtg tgttgactcc
     3361 gcgtgcctcg ttggagccgg tggaaattgg cggggtggtc gttcgcaatg caaccttgca
     3421 taactttgat tacatcgaag agaaagacat ccgcatcgga gaccgggtcc tggtcaaacg
     3481 cgcgggtgaa gtgattccgt atgtcatcgg tccggtcgta gatgcgcgct cgggcaaaga
     3541 gaagaaatac aagccgcctt caaaatgccc agcatgtggg caagacgtag aacacatcga
     3601 aggcgaagtg gcttggtact gcgtcaatgc cgcttgcccg gcacagttag tccgcaacgt
     3661 ggagcatttc gtctcgcgcg gcgcgatgga tattgtcggc ttgggcatca agatcgtgga
     3721 acaattaatc gaagccggtc tggtcaatga tgtagcggac ctgttcacac taaagaagaa
     3781 agacctgctt gagttggaag gctttgccga gaaaaaggcg gagaatctgc tcgggtcgat
     3841 tgagcaggca aagggtcaga gtttgaaccg cttgctcgct gcgttaggaa tccacggtgt
     3901 gggcgaagtc atggctggcg atttggcacg cacatatggc aatctatccg cattatcaaa
     3961 gacaactgcc gatgaacttc agcagatcga aggcgtgggt ccgaacatcg ccgaatctat
     4021 tgtggattgg ttcgctcgcc cggcaaacca aaaattattg aagaagttaa agtcggctgg
     4081 cgtagacccg caaatgcaaa aggacgaaaa gaaaaaagaa ggcgccttca ctggcttgac
     4141 ctttgtagtg acgggaacct tgccgaattt ctcgcgtgac gatgccaaag aattcattga
     4201 gaacaacggc ggtaaagtga ccgatagtgt aagcaagaaa accagctatt tggtgcttgg
     4261 cgaaaacccc ggctccaagt tcgataaggc gaagtctttg ggggtaaaaa tcattgacga
     4321 agccgaattg aaaaaattag caagctaaac acaagtgaca gtcaccttaa gggtgactgt
     4381 cacttttaag ctagacagaa aacaccccct cggctaaact tgcgccatcc cttttggagg
     4441 ctctttcatg gattttcata gcattgacaa taacagcacc cggcgcattg ccgctctcaa
     4501 acaccgtgta gacaccctac gcgaaaacaa tcattatttt tacaaccaag ccgtggaaga
     4561 aatgctcccc ggcgccaaag tgcgcgtaaa tgggcgcgtg atgggcatgt acgcctctta
     4621 ctcctacttg ggcttggtcg gtcacccacg cattaacgaa gccgccaaac gcgccgtaga
     4681 caaattcggc accggcacca acggcgtgcg caccttggct ggaaccctca ctattcacaa
     4741 cgaactcgaa gaaaccattt caaacttcaa gcatgcagaa gccgccatta cctatacttc
     4801 tgggtatgtc accaacctga ccgtcatctc taccttgatg ggacgcggcg actacgtctt
     4861 ctcagacaag atcaaccacg cctccatcgt ggacggttgt ttgatgtctg gtgcggaatt
     4921 ccgccgtttc cgccacaacg acatggaaca tctcgaaggc ttgctcaaga acgctcctgc
     4981 cgatgtctcc aagctcgtca tcgcggactc ggtcttcagt atggacggcg atatcatcga
     5041 cctgcccaag atcgtggagc ttagcaaaaa atacaacgcc tggctcatga tcgacgaagc
     5101 gcactcggtg ggcgtgctcg gcaagaccgg caccggtatt gaagaacact tcaacatgta
     5161 cggctccatc gacatcaaga tgggtaccct cagcaagacc atcccttcgg ttggcggata
     5221 tgtggctggc aaaaaagaac tgatcgatta cctgcgccac ggcagccgtg cgtacatctt
     5281 ctcggcagcg ctcccacctg ctcaggcagc cgccgcgaac gaagccttca aggtcattct
     5341 cgatgaaccc gaacgcctcg accgcttgaa cgccaacact caacaattta ttggcggctt
     5401 gaagagcatg ggctttgata ccttgctcac cgaaactgcc atcgtccctg tgctgtgcgg
     5461 caccgatgaa aaagccatgg aattgaccaa acgctgccaa gagcaggatg tcttcgttct
     5521 gcccgtcgtc tcgcctgcag tgcaagaggg catgtctcgc ttgcgtgcca cggtcactgc
     5581 ggcacacgaa cctgcagata ttgaacacgc tatggatgtg atctaccaat ctggcaaagc
     5641 catgggaatg gtcaagtaat tagatagtta gataaagttg aaaatcgcct gtcctatcga
     5701 caggcgattt ttttatcggc gttcgctgct gcttggagag acagaccaag ccccagcgga
     5761 gtgggaagcg gggttttcat cctgatagtt cttgatagaa tttttataac atttttctaa
     5821 taaattcctt gtacaatgcc gtcaacgtta caaggagcaa actatgaaat ggcaatggaa
     5881 atttggaaca tttgcaggaa ttgatgtcta catccatgcc acctttttac ttttgatcgg
     5941 gtgggttggg tatagtcatt ggttggaaaa aaatagctgg ttcgatgtgt tgagcggcat
     6001 tggctttatt ctcgcgctct ttttatgcgt actcttgcat gaatatgggc acgcgctgac
     6061 cgctcgtaaa tacggaatca agacgcgcga tatcacactc tacccgattg gtggcgtggc
     6121 gcgtttggaa cgcatgcctg agaaaccgat cgaggaattg tgggtggcgt tgatgggacc
     6181 cgccgttaac gtggtgattg ccgccattct ttttgcggtt cttttactga ccggaaacct
     6241 ggtgccgctc accgaacttg gggtggcatc tggaagtttt ctcgagcgcg tgatggtggt
     6301 gaacgtttca ctggtattgt tcaacctcat ccccgccttc cccatggacg gtggacgggt
     6361 gttgcgagcc atcctcgcca tgcgcatgga ctatgtgaaa gcaacacaga tcgccgcaag
     6421 tattggtcag ggactggcgt tgttgttcgg gttgatcggt ttgttcggga atgcgagctt
     6481 gttattcatc gccttcttta tttggatcgg tgcttcacaa gaagccagcg ccgtgcagat
     6541 gaaaaatgtc atcagcggca ttccagtggg acgcgccatg ttgaccgatt acaaaagcct
     6601 ctcaccgcgt gacccgctct cgcgcatggc acagttggtt ctggcaggtt cacagcacga
     6661 cttccccgtg atcgacgacg cagaacgcat cgtcggcgta gtgacccgcg acgatttcct
     6721 cgccgcactc acccaacacg gacaaaacat cgccgtctca gcagtgatgc gtaacaatcc
     6781 gcctgaagtg gattcatacg atatggtgga agttgccctg acgcgtattc aagagtcggg
     6841 cttccccacg ttgccggtta ctcattccgg tcagctggtt ggcattatca ctgccgagaa
     6901 tatcaccgaa tatttgatga tccgcacggc attgcgaacc gcccaagtgg tttcgattta
     6961 atgagggtct ctgcaagtgc agatgagaga tttcttaacc acgaaaatcg ttttttccat
     7021 atactaggca caactccgat ggggagtagc cgcccacatg ttgggtagct cagccgtcaa
     7081 tacgtggtac attgtgccac cggctgttct gtaaaggcaa gaccatcgtc aacatccttg
     7141 acgatggtct ttttatttgc ggaaagtatt acgccttcct caaacggcac gtcaggttaa
     7201 atacatcaaa atagattaaa cttattagga acacggaaac aatacatgaa ctggcataca
     7261 atcgaaacca atgcagcttt gaacgaactt gcttcctctt ccgaaacggg tctgacttcc
     7321 gcacaggttg aggaaagcaa aaagaaacac ggcgcgaacg agctcatcga acgcggcgga
     7381 cgcaccccat ggcaaattct gtgggaacag atcaccgcca ccatggtgtt gattctcatt
     7441 gcagccgccg ttgtggcagg gttgttgggc gatacaaaga acaccatcgc cattctttcc
     7501 attgtggcgt tgtacgcctt gctcggcttc attcaagaat atcgcgccga acaagccatt
     7561 gccgcgctca agaaaatgtc ggtacccaat gtgcgtgtga tacgcgacgg caaaatgcag
     7621 gaactctctg ctcgcgagct tgtgcccggc gatatcattc aactcgaaac tggcaacgtc
     7681 atccctgcag atttacgttt ggttgaagcc gtcaacttga gaattcaaga agccgcgctc
     7741 actggcgaat cagaagccat tgaaaaacac gtggctgcac tttcaaatga agaccttccg
     7801 cttggcgacc gtcgcaacat ggcatacatg ggcacggtca tcacccaagg gcgcggactt
     7861 gctctcgtca ctgccaccgg catgaacacc gagcttggca agatcgcaga tatgatccaa
     7921 gcggtcaaac aagaacagac tccgcttcaa cgtcgccttg atgcgcttgg taaaaccctt
     7981 gcgctcattg gcgtgggcat tgcggtgctc attttcattc tcggtattat tcgtggcgac
     8041 gaagttcgtc acatgttact cacggcggtc agtgttgcag ttgccattgt gccagaaggc
     8101 ttacctgccg ttgtcaccat cacactcgcg ctcggtgccc aacgtatgtt gcagcgtcag
     8161 gcgctcattc gcaaactccc cgctgtcgaa accctcggct cggtcactgt catctgctcc
     8221 gataagaccg gtacactcac tgaaaaccgg atgacggtcg ttgtgttgga tgtggcggaa
     8281 catgccctcg acctgaccgg tgacgttgcc cgcgacggct cgcttcatgc gacccgtggg
     8341 ctgggtgcac ctgcccaatc ctctctctca ctggcagcga ttggtggcgc gttgtgcaac
     8401 gatgccaaat tagtggacga aggcgatgac cgcttccaca ccatgggcga ccccacagaa
     8461 ggtgcgctcg tagttgccgc cgcaaagatg ggatattgga aatcttccct cgattcatct
     8521 ttcccccgtg ccgccgaatt accctttgac tctgaccgca aacgtatgac caccgtgcat
     8581 caccttggtc aatacgaccc gaccgtgctt tctggtttag agatcggcga tacacgttat
     8641 atcgctttca ccaaaggcgg tgtggacggt ctgctagaca ttgcaagcca cgtctgggca
     8701 gacggcaagt ctgttccgct cgatgatgcc tggaggtcca agattgaagc gtcgaacgaa
     8761 cgccttgcca gaaaaggtat gcgcgtcctc ggcgttggct tccgccttat caatgccatc
     8821 cctgagatca ttcaaactga ccttgaacag aatatcacct tggtaggttt gttcggcatg
     8881 atcgacccac cacgtaacga ggtcaaagat gcggttgcca tcgccaagac ggctggcatt
     8941 cgccctatta tgattactgg tgaccatcca ctgaccgcga tggaaattgc acgtcaattg
     9001 ggcatcaccg ataatggacg tgtgctgact ggcgttgagc ttgaaaaaat cacgatggat
     9061 gaattaaaga ccatgctcga cgatgtgaac gtctttgcac gtgtagcacc cgagcataaa
     9121 ctgcgcatcg tgcaggcatt gcaggaaaag ggtcatgtcg ctgccatgac cggtgatggc
     9181 gtgaacgatg ccccggctct gcgcaaagct gatattggtg tcgcgatggg catcaccggc
     9241 accgatgttt ccaaagaagc atcagacctc gttctgctcg atgacaactt cgcgaccatt
     9301 gtggcggcgg tcaaagaagg tcgtactatt tacgacaaca tccgcaaatt tgtgcgcttt
     9361 agtgtagctg gcaacatcgg taaagtatta gtcatgctca ttgcgccttt ccttggtaag
     9421 ccattgccgc tcttgccttt gcagttactg tggctcaacc tgctaactga tggtttgctc
     9481 ggtttaggga tgggcgtgga aaatcctgaa aaggatacga tgaagcgcaa accttactcc
     9541 ccgactgagg gcgtcttctc gcgcggggct ggcatacaaa cgatttgggt tggcgccctc
     9601 ataggcgcac tcgctcttgg gcttggctcc tggtattact ttactgaccg ccccgaatgg
     9661 cagacgatga tcttcacctc tctggcattc atgcagatct tccaagcgct ggcgtctcgc
     9721 tcagacaaag actcgctcgt caagatcggc gtgatgagta accccgttct ggcgggcatg
     9781 gcgctgttgg tctttgttct tcagatggcg gtcatctaca tccctgcatt ggcaaacttc
     9841 tttgaagtca ttcctctacg ctggcatgat ctatctatcg ccgcaggaac aggcttgctg
     9901 gtattcctcg ttatggaagt cagcaaatgg tttaagaaat aacttgttag aaaataaaag
     9961 acctgctttt gaagcaggtc ttttttgtta gataagaata gggctgtttc cgcgtaaatg
    10021 gtctgggcgg gtaagcaacc ataagaccca atcagcaaca tggtctggca tgaggtttgg
    10081 ttttccttcc acatcaaaag gcgcagatac cgccacgccc ggcgaaagaa tatgtgtacg
    10141 aatgccatgc tcgctattct cagcgcgaat gagctccatc aacgttgtca gtccgtgcat
    10201 ggcaacggca aatgccccat cttgtgggta tacgcccaaa gcagagtctg agccgattgc
    10261 catcacctca ccatgttttt gttctcgcaa aaaagggagc gccgcacgcg acatcaaaaa
    10321 cggctcacgt aaattggcat ccatcaccag atcccaggtc ttgacgctgt gactgtgaat
    10381 catgccaccg ctccaaaagg gcgagataag gatcaggata tccaaaccgc caaattcagc
    10441 cgtggtttga tggacaaccc cttccgcctc ttccaacgac ttgaggttgg attctacaaa
    10501 gaagccttta cctcccgctt gcttgactct gtctactgcc atcgctaata aatctgcctg
    10561 tctgccacat aaacagatat tcgcccctgc ttctgccagc ttcaatgcaa cagcacttgc
    10621 tatttctgtg gagccacccg taatgatggc ggtcttgccc gataaagatg ccatgctatt
    10681 tgctttcctt gatgatggtc gtccctgcta ccgcatcact gaccgcatca ccaatcaggt
    10741 tgatcgaaag caccgcgagg aaaattccca accccgggaa gaaggtcaac caccacgcct
    10801 ggcggacgta tttcatgccg gcattgagca tagcacccca ttctgctgtg ggcggctctg
    10861 caccgagccc aagaaagttc aacgccgcac ccgcaaggat agatgagcct atcccaagtg
    10921 tgatgagcac caacaaagtc cgaatcgagt tcggcaaaat atgcttgagc atgattcgcg
    10981 tgtctgaact gccgagtgct ttcgcggctt cgatgtaagt catttcacgc aaggcaaaca
    11041 cacttgaacg cgtaagacgc ataaacgaag ggatgaacga gataccaatc gccaacatag
    11101 cgttgttgat accgggtccg agaatggcga tgatggctaa tgccagcaaa atatcaggga
    11161 acgccatcag cacatcattg aaccaagaga tgaacgaatc cagccatccg ccgaagtagc
    11221 cagagatcaa cccgagcaga atcccaatca atgcgccgat gatgataccg gtcagcccaa
    11281 ctcgcaatga cagttgaccg ccataaagga agcgggtgaa gttatcacgc ccaaggttat
    11341 ctgtgcccat ggggtattca aagctaggcg cttctaaggc tacagatggg gcgtttttat
    11401 tggggtcttt cgctgtgata aaaggtgcgg catacgaagt gacgattaaa agaattaata
    11461 ggataccgcc aaattgggcg ctacgattac gaagaatctg cctgaacaaa cgaatgcccg
    11521 gcgaattgga tttgatggga gtggatgaca agtttgcggt catttgaggc gaatcttcgg
    11581 gtttagtaac acataggtga tgtctgtaat ggtgttgatg agcacataga ttgtagctgt
    11641 aatcagcacc acgccttgca ccatggggta atcacggctg tagatggctt gcacggctaa
    11701 acgcccaatt ccctgacggg caaacatggt ttccacaatg gttgcgccgc caagcaaata
    11761 cgccatctgc agaccgacaa aggtcacaac aggaattaat gcatttcgta atgcatgttt
    11821 gataatcacg gaacgttcga acagtccctt ggcgcgggcg gtagtgatgt attctttatt
    11881 gagcacatcg agcatgctgg cacgtaccat gcgcgcgacg actgcggctt cggggagtcc
    11941 caacgtcagg gcggggagaa agagatcggc gaaggaagag ccgttggaaa tgcttgggaa
    12001 gagttttaac ttcacagaga agaaaaggat gaggagcaga cccatccaaa aagtgggtat
    12061 ggaaagtcca ctaacagata gacccatcac aatcgtatcg acccatgtac catgacgtaa
    12121 tgccgccagc aaaccaaaga ttgcgccaat gctgagtgcc accgccaatg cagacattgc
    12181 caaagagaat gtgccgggca gttgttcgct gatggatgca gaaacggatt gattcgtgcg
    12241 gatcgatttc cccaagtctc cgcgcaatgc cccagagacg taacgtccat actgcacgta
    12301 aagcgggtca ttgagcccga gttgctcgcg cagttctgcc actttttctt cattcacttt
    12361 ttggcgtcca aggatcgcca tggtgacatc gcccggcacg aggtgaatga ccgaaaagac
    12421 caacaccgaa atgccaaaga gtaccaagac cgatgtaaaa agacgccgta tcaaataatt
    12481 gagcatggaa agcctggagt ttttaagtgt gccgcacccc tgtaaagaga tgcggcacac
    12541 ccattgtatt acttatttgc cgaacatgct cgttgaaaga gcaaaggt
//
LOCUS       DAOQFT010000003        11783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_2530_length_11783_cov_6.427439, whole genome shotgun sequence.
ACCESSION   DAOQFT010000003 DAOQFT010000000
VERSION     DAOQFT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2530_length_11783_cov_6.427439"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLR93_00115"
     CDS             complement(<1..759)
                     /locus_tag="PLR93_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH25387.1"
                     /translation="MRKSWLVWILPFLFVLESCGTIQPATTPDARPNASLTDPLPARF
                     SMGPTTIPYGKNIRFEQISLEEGLSQSVVNVILQDQKGFLWIGTDDGLNRYDGYNFKI
                     FKPDTTNPFSLSDRSITDIVEDKDGNLWIATRMGGLNRYDPISGKFTQYMHSKNDATS
                     IISNQVTSLFVDKTGLWVGTNNGLDFLDFSSNQFIHYPLATGKLVPESKSISVVYKDS
                     ANRLWIGTSDAGLGLYDEKDQSFKSFKNNEYNNQS"
     gene            1142..3733
                     /locus_tag="PLR93_00120"
     CDS             1142..3733
                     /locus_tag="PLR93_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH25388.1"
                     /translation="MKIKKAFNALSLLVILGMLLAACAPAATDAPVEAPVATDEAMAT
                     DEPAPAATEAAPATTRHGGWLDEIDFSVVSSDSAVSQLQAGAIDFYSFNLSSNTLQDI
                     KDAGLNYTQSYGGNYGISLNPAVFDDTTVLNPFSNRKIREAVNWLIDRNYINQEIYGG
                     GSLPKLLPITTQLVEYTNLIDTARALETKYAYNLERAREQVAAEMEAMGATMSSDGKW
                     QFNGEPVTLIFLIRSDGDGTRKPMGDYVANQLEEVGFTVDRQYKTSSEAFPIWLGSVA
                     SEGQWHLYTAGYLPSGLTRDERANIQQYYLPTSIQASDPFISNVADPELQKLGDDLAQ
                     GNFTSKEERDAMLARALDLALQDSLFVWVVDQLVYAPYNSNVSVTYDLAAGYEAAFMG
                     NYNLRFVDQEGGTMKVGTNDLFTQPWNTMAGSNWVWDSNIMRATSMGVASSGSASSGL
                     MGDPYTGLAWPQRIESASLEYQTGLPITDNKLGWVDVQAVDEIPVPEDAWVDWDATTQ
                     TFITVGQKYPDGTTAKVKSTVVYPADLFETVKWHDGSPISVADFVMSFVIFLDRGKPE
                     SAVYDEAAALSVDAFLPSFKGWRITSTAPLTIEYYSDAYSADAELNVAPLWPGSPSGL
                     SGENSWQILAISNLAEAAGELAYSSDKADANSIEQMSWVGGPSLEILKKYLDQAQTES
                     YVPYEATLGQFLTPEEIALRYENFSKWYADHGHFWVGTGPYYLDQVFTTEKSLVLKSF
                     ADFPDLADRWSSFSDPKRARVILDGPGQVTAGQEATFDVTVNFGDEAYPTADIKQVKY
                     ILYGTTGDVLEVGNAEAVGEGQFQVALSADATSKLETGSAKLEVAVVPIPVAIPAFTS
                     FDFVVVK"
     gene            3803..>4662
                     /locus_tag="PLR93_00125"
     CDS             3803..>4662
                     /locus_tag="PLR93_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25389.1"
                     /translation="MSVVAPAASEQTAPKRKTALHALSRMLRYIAMRVLTLFTTVVIG
                     VYLTILIANMGGYVDQIIRGQIRDTITQSVANNPTLRNMDPETRRKLIEEQITLEEKR
                     RGLDTPIAIRNFRHLTQALQLDLGRAFNITSDSGSKQVRLILLERLPATLLLMGTSQL
                     FLFFSSIYIALNLSRNYGNIWDKLVIALSPTSAIPSWFYGIFLILIFAAGFKVLPFGG
                     MVSSPPPLNRFDYIISVLQHLVLPGGALVISSFFLSVYNYRTFFLIYSSEDYVDMARA
                     KGLHAKDIERR"
     assembly_gap    4663..4762
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4763..5803
                     /locus_tag="PLR93_00130"
     CDS             <4763..5803
                     /locus_tag="PLR93_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25390.1"
                     /translation="TYTMTYEFDFKYDVYPQDMIVYVNALFDQKQPFISMEWITPDGR
                     TIRVSNFATAAKSTYRFSQDDKLAAKLRAEDPIPALFSDPDTGEVLKGPYKLVITGTT
                     FEPNSNIDVELVVHGQVYGLAGTDHARRDLTLPLLWGAPVALAFGLIAAIGTSVLTMI
                     IAAVGTWYGGWVDQLVQRITEVNLVLPFFAIMIMIGTFYSRSIWTIISATIVLNIFTG
                     AILGYRAIFLQVKESMYIEAAKAYGATNWRIIFHYLIPRMIPLLIPGLVQAVPAFVFL
                     EASLAVIGLGDPVLPTWGKIIQDAQANGALYKGYYYWVLEPAVLLIITGLAFAFLGFV
                     LDRIFNPRLRDV"
     gene            5817..6776
                     /locus_tag="PLR93_00135"
     CDS             5817..6776
                     /locus_tag="PLR93_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25391.1"
                     /translation="MTELTPLLEVKDLKLHFRTQKGVVQAVDGVDLDLGFHRAVVILG
                     ESGCGKTSLSKAMLRLLPRNVAKYSGELVINGKDVMPFTDEEYRQNVRWVSMSLVPQA
                     AMNALNPVITVGEQVSEPAMLHLGLNKDEALGMAYKMFQHVGIPADFISRYPFELSGG
                     MRQRVALAMALVTSPNLIILDEPTSALDLLTQANIMNVLKRIKHELGTSYILITHDIA
                     TSSELADEVAIMYAGQIVETGDAKDFFPAPLHPYSQMLMASVPRLRSESDPMFITGQP
                     PSLMNPPTGCRFAARCPFRFEKCDQEPPVFQKGNRKVKCWLHQ"
     gene            6794..7372
                     /locus_tag="PLR93_00140"
     CDS             6794..7372
                     /locus_tag="PLR93_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH25392.1"
                     /translation="MPSLALYDVLYSFKDIHKVVLEFASELNEEQLRWRPRGYSTSIG
                     FHLWHLARESDFLKAIILERTPQLGSDFGEPKEIWAVEKLAERWRFPTDLNATVGTGL
                     SDEIAATLPIPPKDELVDYLQKSYEALETFIALLDERYPTFEGVEEDLQRKLKNIRLN
                     LLVFLSHDCRHLGMMECLKGLQTGFGSATEKR"
     gene            7419..8420
                     /locus_tag="PLR93_00145"
     CDS             7419..8420
                     /locus_tag="PLR93_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25393.1"
                     /translation="MSVKIDEPTETLLAIHNLHVWYELRRFGFSVVGHVKAVNNVSFE
                     LGKGETVAVVGESGCGKSSLMKTILGLNVPTGGELLFEGKRLTALTEAELRSYRFEIG
                     YVQQDPYGALPPFMNVEQILEEPLIISGVSSKDERRERIHKILGEVKLHPVEDFLHKY
                     PHMLSGGQQQRVVIARAMVLEPKMIVADEPVSMLDASVRVEILKLMHALQEQHHLSVI
                     YITHDLSTVKYFSARIFVMYAGSLIEKAETRQLLKNPLHPYTHALLAATSDPNAANAE
                     VYKAVPPGEPPSLADPPKGCRFHPRCAKAMTGVCDVKEPPDFEPEPNHFVACWLYEA"
     gene            8392..8757
                     /locus_tag="PLR93_00150"
     CDS             8392..8757
                     /locus_tag="PLR93_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25394.1"
                     /translation="MWLVGYMRHDDKKPLLHVLGLAFASVGCCAPVSDDYKSGRTNVF
                     SRFLDLWLIHRWLGAWHVWVNALVCYGYQKETTTDRRLAANGIKNASPFTARHFYKKY
                     LCFGFIDGAEAVDEVDSQP"
     gene            complement(8698..9732)
                     /locus_tag="PLR93_00155"
     CDS             complement(8698..9732)
                     /locus_tag="PLR93_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNH25395.1"
                     /translation="MPKTQNRAVMTWLYVFAGVVAFLVVFGGFTRLTRSGLSIVEWNP
                     INGTVPPMAQHQWEAEFEKYKATPEYQLINTDMTLQEYKYIFYVEWFHRLVARMAGLF
                     YALPVFYFLIRKTIPLKEFGIYFWMGVLFIGQAVMGWLMVYSGLVDRPAVSHFRLTAH
                     LLLALTLFSLAIWTATGHRLGFHHYEKKAKWSLPTKISVAALVVLAIQISYGGMTAGL
                     KAGHVSDTWPLMFGKLIPPNLFNSFINILETPQTIVFIHRWFAWLGVILVPVVFYIIK
                     KQNYPADIQKGLLWLLGVVALQITLGVLTILSYVNIVIALLHQANAILLLGLAVYFIH
                     RFRAVDEAKA"
     gene            complement(9874..>11783)
                     /locus_tag="PLR93_00160"
     CDS             complement(9874..>11783)
                     /locus_tag="PLR93_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH25396.1"
                     /translation="RLAQVHERLGHGQQAAMEYLAIASILQRAGNPEKTQEMVNKAQS
                     LVPNSAEVKQAQTLLRTGQLLPKPIRAKGGTGPIRMAQVKQLQEPAKKAASGLDPIAE
                     ARQKALTQLAEVLFDLSDDSSPVAQDRKGISAIMRGTGSLSAQSEQLKVVLHLGQAID
                     AQSKGNDTLAAEEMEAALNAGFSSSALSFSIGYLRFKNERYESAQRALQNSVKHEDYG
                     LASRLLLGDIISRKGDFRDAATQYLEALKFADAATVPLEQGDDIRQQYEPLIESYQQQ
                     TDEAVLRKVCDNIRGLLMRADWRDQLHKTRDQMPKQDGMAAPLADMILQVQSSSVLES
                     MNRINQLARMGSLRSAMDEAYDAIQHAPTYLPLHIIMGDLLVQEGHTTDAIAKFSVVA
                     HSYSVRGEVMQASKLLRRIIQISPMDLGARNRLIDQLVARGQVDDAIQEYLELAAIYY
                     RLAELDMARKTYTHALRIVQQGNASRDWNTHILQRMADIDMQRLDWKQALRVYEQIRT
                     LMPDDDASRKQLIELNMRMGQQDRAMSELESYITHLESQNKQELAIAFLEELARDHED
                     QPLIKRTLAALLHRSGRIGDAIPILDQLGEALLEKGDKQGAMEVINQIVLMNPPNVQD
                     YRVLLSQMQNS"
BASE COUNT         2830 a         2685 c         2985 g         3183 t
ORIGIN      
        1 gctctgattg ttgtactcat tatttttaaa cgacttgaaa gactggtctt tctcatcata
       61 aagtcctaag ccagcgtctg atgtgccaat ccaaagcctg ttggcagagt ctttatatac
      121 aacagaaatg gatttgcttt cagggacgag cttgcccgta gcgagcgggt agtgaatgaa
      181 ttggttggat gagaaatcaa gaaaatcgag cccattgttt gtgccaaccc aaagcccggt
      241 tttatctaca aaaagtgatg tgacttgatt gctgataata ctggtcgcat cgtttttgct
      301 atgcatgtat tgagtgaact tacccgagat ggggtcgtaa cgatttaatc cccccatacg
      361 agtggcgatc cacaaatttc catctttgtc ttcaacaata tcagtgatcg aacggtcact
      421 taaactgaat ggattggtag tgtcaggttt gaatatcttg aagttgtagc catcataacg
      481 gttcaacccg tcatcggtgc cgatccataa aaaacctttt tggtcttgca agattacatt
      541 tacaacactt tgcgaaagac cctcttcaag actaatctgt tcaaagcgga tgttctttcc
      601 gtatgggatg gtggtaggac ccatagagaa gcgagcaggt aacgggtctg ttagagaggc
      661 gtttggtctt gcatccggcg tggtggcagg ctgaatcgtc ccacaagatt ccaaaacaaa
      721 aaggaagggg agtatccata caagccacga tttacgcata gtgaaactat tataagcaat
      781 ttatgagctg ggattgaaag gcggctccct ctgggggagg tctgtttcac cctaccggag
      841 aatggttttt cgctgtgaga tgggttgtga aacatttttc agtggatggc tttagaacca
      901 ttcaattgtg ggaggaaaac caattgtctg gatgcatgac agttgccctt ggaattcgta
      961 tgataaggcg catgtggaat tgttctcctc caagaagaat caaattccct cagaaggcgg
     1021 agtatcagtg agcagactgg ttttccgcct tctgcttaga cgaattcatt aggaggtgct
     1081 agctgaccta tcttgtacgc tgttcaattt gttttccaat ttcattaagg agaaaaagag
     1141 aatgaaaata aaaaaagcgt ttaatgcact cagcttattg gtcatccttg gcatgttgct
     1201 ggctgcttgt gcgcctgccg ctaccgatgc cccagtggaa gcacccgttg caaccgatga
     1261 agcaatggca acggatgaac ctgcacctgc ggctacagaa gcggcacctg ctacgacccg
     1321 tcacggcggc tggttggacg aaattgattt ttcagtcgtt tcatctgatt cggcagtttc
     1381 tcaattgcaa gccggtgcaa ttgacttcta ttcgtttaac ctgtcttcaa acaccttgca
     1441 agatattaaa gatgccggct tgaactatac ccaatcgtat ggtggtaact atggcatcag
     1501 tttaaaccct gccgtgttcg atgacacaac ggtcttgaat cccttctcga accgcaagat
     1561 ccgtgaagcg gtgaactggt tgatcgaccg taactatatc aatcaggaaa tctatggcgg
     1621 cggttcctta ccaaaacttc tgcctatcac aacccagttg gttgaataca ccaatttgat
     1681 cgataccgct cgcgctcttg aaaccaagta tgcctacaac cttgagagag ctcgcgaaca
     1741 ggttgccgct gaaatggaag ccatgggtgc cacaatgagc tctgatggca aatggcagtt
     1801 taatggggaa cctgtcacct tgatcttcct tatccgctcc gatggcgatg gcacccgcaa
     1861 acccatgggc gattatgttg ccaaccagtt ggaagaagtg ggctttactg tagaccgtca
     1921 atataagaca tcttctgaag ccttcccaat ctggcttggt tccgtggctt ctgaaggaca
     1981 gtggcatctt tatactgctg gttatttgcc ctctggtctt acccgcgatg agcgcgcgaa
     2041 cattcagcaa tactatcttc ccaccagtat tcaggcgagc gacccgttca tctccaatgt
     2101 agcggacccc gaacttcaga aacttggtga tgatcttgct caaggtaact tcactagtaa
     2161 agaagaacgt gatgccatgt tggcgcgcgc cttagacctt gcccttcaag actcactctt
     2221 tgtatgggtt gtagatcaac tggtctatgc tccttacaac agcaatgtct cggtgaccta
     2281 tgacctggca gctggttacg aagcggcatt tatggggaat tacaaccttc gttttgtgga
     2341 ccaagaaggt ggcacaatga aggtcggtac gaatgacctc ttcactcagc cttggaacac
     2401 aatggcgggc agtaactggg tgtgggactc gaacatcatg cgtgctacca gcatgggtgt
     2461 ggcaagctct ggcagcgcct cttctggctt gatgggtgac ccatacaccg ggcttgcctg
     2521 gccgcaacgt attgaaagcg cctctcttga atatcaaact ggtttaccca ttactgataa
     2581 caaacttggc tgggtagatg ttcaagctgt ggatgaaatt ccggttccgg aagatgcttg
     2641 ggtcgattgg gatgcaacga ctcagacctt cattacagtg ggtcaaaaat acccagatgg
     2701 caccactgcc aaggttaaga gcacggtggt gtaccctgcc gatttgtttg aaaccgtcaa
     2761 atggcatgat ggcagcccaa tctctgtcgc agattttgtc atgtcgttcg tcattttcct
     2821 tgatcgcggt aagcctgaaa gtgcagtcta tgatgaagcg gcggctcttt ccgtggatgc
     2881 tttcctacct tccttcaaag gttggcgtat cacctctacc gccccgctca ccattgaata
     2941 ctactctgat gcgtactctg ctgatgctga gttgaatgtt gctccattgt ggcccggttc
     3001 cccgtcaggg ctttctggtg agaatagctg gcaaattttg gcgatctcca atctggcaga
     3061 agctgctggc gaattagctt actcttccga taaggcagat gctaatagca ttgagcaaat
     3121 gagctgggta ggcggaccaa gccttgagat cctcaagaag tatttggacc aggctcagac
     3181 cgaaagctat gttccgtatg aagcgacctt gggtcagttc ctgactccgg aagaaatcgc
     3241 tcttcgctat gaaaatttct ccaaatggta tgccgaccat ggacatttct gggtgggaac
     3301 tggtccgtac tatctcgatc aggtctttac tacagagaag tctctcgtgc tcaagagctt
     3361 tgcagacttc cccgatcttg ctgaccgctg gtcttctttc agcgacccga aacgggcacg
     3421 tgtcatctta gatggaccgg gtcaggtaac ggcggggcaa gaagcgacct tcgatgtgac
     3481 cgtcaacttt ggcgatgaag cgtatccgac tgccgatatc aagcaggtga aatacatttt
     3541 gtatggcacc acaggcgatg ttcttgaagt tggaaatgct gaagcagtgg gcgagggaca
     3601 gttccaagtt gccttgagtg ccgacgcgac ctccaaactg gaaaccggtt ccgccaaact
     3661 cgaagtggca gttgtaccga tcccagtggc tatccctgct ttcacttcct ttgatttcgt
     3721 agttgtaaag taagttgatg tagaaattcc aataattcag ggtgaggcat ttcgcctcac
     3781 cctgaaccct tgcgaggtat ttatgtccgt tgttgctccc gctgcttctg aacagaccgc
     3841 tccaaagaga aaaaccgccc ttcatgctct aagccgtatg ttgcgttaca tcgccatgcg
     3901 tgtgttgacc ttatttacta ctgtggtgat cggggtttat cttacgatct taatcgcaaa
     3961 tatgggcggg tatgtagacc agatcattcg ggggcagatc cgcgatacga tcacacaatc
     4021 tgttgccaat aatcccaccc tgcgcaacat ggacccagaa acacgccgga agttgatcga
     4081 ggaacagatc acacttgaag aaaaaagacg cggactggat actccgatag ccatacgtaa
     4141 ctttcgccac ttaacgcaag cacttcaact tgatcttggg cgtgccttca acattaccag
     4201 tgacagcggc tcgaaacaag ttcggctcat tttgttggaa cgactgcctg ctacattatt
     4261 actaatgggc acatcgcaat tattcctttt cttttctagt atctatattg cactgaatct
     4321 ttctagaaat tatggaaaca tatgggacaa gcttgtaatt gcactatcgc ccacctcagc
     4381 aattccctct tggttttacg gtattttctt aattctgatc tttgcggcgg gctttaaagt
     4441 ccttccgttt ggtggcatgg tctcttcgcc gccgccgtta aatcgctttg attatattat
     4501 cagtgttcta cagcacctag tcttacctgg tggtgctctg gtcattagtt cattttttct
     4561 tagtgtttac aactatcgaa ctttcttttt gatttattcc agcgaagatt atgttgatat
     4621 ggcacgtgcc aaaggcttgc atgccaaaga cattgagcgc cgnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnacatacac aatgacatac gagttcgatt ttaagtacga
     4801 tgtctaccct caagatatga tcgtttatgt caatgccctt tttgaccaga aacagccgtt
     4861 catttcgatg gagtggatca cacctgatgg gcgaacgatt cgtgtttcaa attttgctac
     4921 cgcagccaag tctacctatc gcttctcaca agacgataaa ctggcagcca aactccgggc
     4981 agaagacccc atcccagcct tgttctctga cccagatacc ggtgaggtat taaagggacc
     5041 ctataagctc gttattactg gcaccacttt tgagcctaac tcgaacatag atgtagaact
     5101 cgtggttcac ggacaggtct atggcttggc agggacggat catgcccgcc gtgatttgac
     5161 cctaccgctt ttgtggggag cccctgtcgc tttggcattt ggtcttattg cagctatcgg
     5221 cacttcggtc ttaacaatga tcattgctgc cgttggcacc tggtatggcg gctgggtcga
     5281 ccagttggtg caacgaatta ccgaagtcaa tttggtgttg ccgttttttg cgatcatgat
     5341 catgatcggt acattttatt cccgtagtat ttggaccatt atcagtgcaa cgattgtctt
     5401 gaatatcttt acgggtgcca ttcttgggta tcgtgccata tttcttcagg tcaaagagtc
     5461 aatgtacatt gaagcggcaa aagcatacgg cgcaacaaat tggcgtatta tttttcatta
     5521 cctcattcca cgcatgatcc ccttgttgat tccgggtttg gttcaagccg tgccagcctt
     5581 tgtgttcctt gaagcgtctc ttgctgttat cggtttagga gacccggtct taccgacttg
     5641 gggcaagatt attcaagatg ctcaggcgaa tggagcttta tacaagggat attactactg
     5701 ggtgttggaa cctgctgttt tgttgattat cactgggttg gcttttgctt tcctcggctt
     5761 tgtattagat cgcatcttca acccgcgcct gcgcgacgtg taaccgagac gtatcaatga
     5821 cagagttgac acccttgctt gaagtaaaag acctgaagct gcacttccgt acccaaaaag
     5881 gagttgtgca agccgttgac ggcgtagacc tcgacttggg gtttcaccga gctgtagtca
     5941 ttctgggtga atcggggtgc ggtaaaacat cgctttcaaa agccatgttg cgcttgctgc
     6001 ctagaaatgt ggcgaaatat agcggcgaac tggtcattaa tggaaaagac gtgatgccgt
     6061 ttaccgatga agaataccgg cagaatgtgc gctgggtgag tatgtctctt gtacctcaag
     6121 cggctatgaa tgcgttgaac ccggtcatta cagtgggaga acaggtttca gagccagcta
     6181 tgttgcatct tggtttgaat aaagatgagg cgcttggcat ggcgtacaaa atgtttcagc
     6241 atgtggggat ccctgctgac ttcattagtc gctatccatt cgagttgagt ggcggtatgc
     6301 ggcaacgggt tgctttggca atggcactcg ttacttcgcc aaatctgatc attttggacg
     6361 aacccacttc tgctttggat ttgctgaccc aagctaatat tatgaatgtg ctcaaacgta
     6421 ttaagcatga attgggtacc tcgtatattc tcattacaca cgatatcgcc acatccagtg
     6481 agcttgcaga tgaagtagca attatgtatg ccggtcagat cgtggaaacg ggcgatgcca
     6541 aagatttctt ccctgcacca ctgcatcctt attcgcaaat gttaatggcg agcgttccac
     6601 gcttacgcag cgaatcagac ccgatgttta ttacagggca gccgcccagt ttgatgaacc
     6661 cgccaacggg gtgtcgcttt gcggcgcgct gtccgttccg ttttgagaaa tgcgatcaag
     6721 agccgccggt tttccaaaaa ggaaaccgta aagtaaagtg ctggctgcac caataaacca
     6781 aggagtttca tccatgccgt cccttgcatt gtatgacgtg ctttattctt tcaaagatat
     6841 tcataaggtc gtgcttgagt ttgcatctga attgaacgaa gaacaactac gctggcgtcc
     6901 gcgaggatat agcacatcta ttgggtttca tctttggcac ttagcccgcg aatctgattt
     6961 tttgaaagcc atcattttag aacgtacccc gcaactcggc agtgactttg gcgagcccaa
     7021 agagatttgg gctgttgaga aattggcaga acgctggcgt tttcctaccg acctcaatgc
     7081 tacagttgga accgggttaa gtgatgaaat tgcagcgact ttgcccatac cgcctaaaga
     7141 cgaattggta gattatctgc agaagtccta tgaagcatta gagacattta tcgccttgtt
     7201 agatgaacgt tatccaacct ttgaaggcgt agaagaagac cttcaacgaa aattgaaaaa
     7261 tattcgcttg aacttgcttg ttttcctttc tcacgattgt cgtcatcttg gaatgatgga
     7321 gtgtttgaag ggtttgcaga cggggtttgg ttctgctact gagaagcgat gaaattcgat
     7381 aaacaactat gatctcagaa ttacaagagg caaaacgcat gtctgtgaaa atagatgaac
     7441 cgacagagac gttgcttgcg attcacaatt tacatgtttg gtatgagctt cgccgctttg
     7501 gctttagcgt ggttgggcat gtcaaagccg taaataacgt cagctttgag ttgggtaaag
     7561 gggaaaccgt agcagtagtc ggggagagtg gatgcggtaa gtcatcgctg atgaagacca
     7621 ttctggggct aaatgtgcca acgggcggtg agcttctttt tgaagggaaa cgtctcaccg
     7681 cattgacaga agccgaattg cgcagctatc gctttgaaat tggatatgtg cagcaagacc
     7741 cctacggcgc tttgcctcca tttatgaatg ttgagcagat cttggaagag ccactcatca
     7801 tcagcggagt ttcaagtaag gacgaacggc gtgagcgtat tcataagata ttgggagaag
     7861 taaagctgca ccctgtcgaa gatttcctgc acaagtatcc acatatgttg agcggcgggc
     7921 agcagcaacg cgtggtgatt gcgcgagcca tggtgttgga accaaagatg attgtggcag
     7981 atgaacccgt ctccatgtta gatgcttcgg tgcgcgtgga aatcttgaag ctcatgcatg
     8041 ctttgcaaga gcaacatcac ctttcagtga tttacattac tcatgacctt tctacagtga
     8101 agtatttttc agcgcgtatt tttgtgatgt atgcaggcag cctcattgaa aaagcagaga
     8161 cgcgccaatt gctaaaaaat ccattgcacc cctatactca tgccttgctt gccgctacct
     8221 cggaccccaa tgctgccaat gctgaggtgt ataaagccgt tccgcccggc gagccgccca
     8281 gtttggcaga cccgcctaag ggatgtcgct ttcatccgcg ttgtgctaaa gcgatgacag
     8341 gtgtttgtga tgtgaaagaa ccaccagatt ttgaacccga gccgaatcat tttgtggctt
     8401 gttggttata tgaggcatga tgataaaaaa ccgcttctac atgttcttgg gcttgctttt
     8461 gcttctgttg ggtgttgtgc tcccgtttct gatgattata aaagtggtcg aaccaacgtt
     8521 ttttctcgtt ttcttgacct ctggctcatc caccgttggc ttggcgcttg gcatgtttgg
     8581 gttaacgcgt tggtctgtta cggctatcaa aaagagacca ccacagatag aagactggcg
     8641 gcaaacggaa taaaaaatgc ctcgccattt acggcgaggc atttttacaa aaagtattta
     8701 tgctttggct tcatcgacgg cgcggaagcg gtggatgaag tagacagcca gccctaaaag
     8761 cagaattgca ttggcttgat gcaacaaggc aatgacgatg ttgacgtatg aaagaatggt
     8821 tagaacacca agcgtgatct gtaaggcaac cactccgagt agccagagca gacccttttg
     8881 aatatcggct gggtaattct gtttcttgat gatgtagaaa actactggca ccaaaatgac
     8941 tcccaaccaa gcgaaccagc ggtgaataaa gacaatggtt tgcggagtct caagaatatt
     9001 gatgaaagaa ttaaataggt tgggcggaat gagtttcccg aacatgaggg gccaagtgtc
     9061 tgagacgtgc ccagctttga gaccggctgt catgccgccg tagctgatct gaatggcaag
     9121 caccacaagt gcggcgacag agatcttcgt aggtaaagac catttggctt ttttctcgta
     9181 gtgatggaac ccaagtcggt gaccggtcgc cgtccagatg gcaaggctga agagggtaag
     9241 agcaagcaag aggtgagcag tgaggcggaa gtgactgaca gcggggcggt ctaccaatcc
     9301 gctgtagacc atcagccagc ccatcacagc ttgaccgatg aagagaacgc ccatccagaa
     9361 gtaaatgcca aattctttga ggggaatggt tttgcgaatg aggaagtaga aaacaggtaa
     9421 ggcatagaac agacctgcca tgcgcgcaac cagacgatga aaccactcta catagaagat
     9481 gtatttatat tcctgcaggg tcatatcggt attgatgagt tgatattcag gggttgcctt
     9541 gtatttctca aattcggctt cccattggtg ctgtgccatg ggaggcacgg tgccgttgat
     9601 gggattccat tctacgatgg aaagaccaga gcgagtgagg cgggtaaaac caccgaagac
     9661 aacaagaaac gccacgactc cggcaaagac gtatagccag gtcatcacgg cgcggttttg
     9721 ggtttttggc atatgattac tccaatttga attggcggca ttatagcatt caggacttag
     9781 gagattgtta gaacttttgt cacgaaagtt gctgtgattt gtcacccggg agaagcgtat
     9841 aaaaaagcgt ggagagcaac tccacgcttt ttgctaacta ttttgcattt ggcttagcag
     9901 taccctgtaa tcttgcacgt tgggtgggtt catcagcaca atctggttga tgacttccat
     9961 ggcgccttgt ttatcccctt tttcaagcaa ggcttcgcct aattggtcga gaatgggtat
    10021 ggcgtctccg atgcgaccgc tacggtggag caacgctgcg agcgtgcgtt tgatcaatgg
    10081 ttggtcttca tggtcacgtg ccaactcttc aaggaaggcg atggcaagtt cttgcttgtt
    10141 ttggctttca aggtgagtga tgtaactttc cagctcgctc atggcgcggt cttgttgccc
    10201 catgcgcata ttgagttcga tcagttgttt gcgagaggca tcgtcatcgg gcatgagggt
    10261 gcggatctgt tcatagacgc gcaaagcctg tttccagtcc aagcgctgca tatcgatatc
    10321 tgccatacgc tgaaggatgt gggtattcca atcgcggctg gcattgcctt gttgcacaat
    10381 gcgtaatgcg tgagtgtatg tcttgcgtgc catatcaagt tcagcgaggc gatagtagat
    10441 ggcggcaagt tcaagatatt cttgaatagc atcatcaacc tgaccacggg ctacaagctg
    10501 atcgatcaga cgattgcgcg cgccgaggtc catgggcgag atttggatga tacggcgtaa
    10561 taacttgctg gcttgcatca cttcgccgcg tacactgtat gaatgtgcca ccacactaaa
    10621 tttggcaatc gcatcggtgg tgtgaccttc ttgtacgagc aaatcgccca tgatgatgtg
    10681 aagcggtagg taggtggggg cgtgttgaat ggcgtcatag gcttcgtcca tggcagagcg
    10741 caggctgccc atgcgtgcca actggttgat gcggttcatc gactcgagca ccgagctgga
    10801 ctgcacctgc aagatcatat ctgccagagg ggcagccatg ccatcttgct tgggcatttg
    10861 gtcgcgggtc ttgtgaagct ggtcgcgcca atctgcacgc attaacagcc cgcgaatgtt
    10921 atcgcagact ttgcgcaaaa cggcttcgtc tgtttgctgt tggtacgact cgatcaacgg
    10981 ctcgtattgc tggcgaatgt catcgccttg ttctagtgga acggtagccg catctgcaaa
    11041 cttcaaggct tccagatatt gcgttgccgc atcgcggaag tcgcctttgc gcgaaatgat
    11101 atcgcctagt aacaggcgcg aggctaaacc ataatcttca tgcttaaccg aattttgcaa
    11161 agcgcgttgg gcgctttcgt agcgttcatt tttgaagcgt aaatagccga tgctgaatga
    11221 aagagctgaa ctgctaaagc cggcattgag cgcggcttcc atttcttcgg cggcaagtgt
    11281 gtcgttgcct ttcgattgcg catcaatggc ttgacccaaa tgcaaaacca ctttgagttg
    11341 ttccgattgc gccgaaaggg agccggttcc gcgcatgatg gcagagatgc ctttgcggtc
    11401 ttgcgccacg gggctactgt cgtcggagag atcgaagagg acttctgcca gctgagtcaa
    11461 cgctttctga cgtgcttccg cgatcgggtc cagccctgag gcggctttct tcgccggttc
    11521 ctgcaattgc ttgacttgtg ccatgcggat gggtcccgtg ccgcctttgg cgcgaattgg
    11581 cttgggcaag agttgaccgg ttcttaataa cgtttgggct tgtttcactt cggcgctgtt
    11641 gggtaccagc gattgggctt tgttcaccat ctcttgcgtt ttttcgggat tgcccgcgcg
    11701 ttgtaaaatg ctggcaatgg caagatattc catggcggct tgttgaccat gccccaaacg
    11761 ttcgtggact tgtgccaggc gtg
//
LOCUS       DAOQFT010000004        11393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_2633_length_11393_cov_6.407479, whole genome shotgun sequence.
ACCESSION   DAOQFT010000004 DAOQFT010000000
VERSION     DAOQFT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2633_length_11393_cov_6.407479"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3359)
                     /locus_tag="PLR93_00165"
     CDS             complement(<1..3359)
                     /locus_tag="PLR93_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH25397.1"
                     /translation="MTIPAAPTTNGKQQGRVKGSLTSTLVRTLLIFTFIPLALMASVA
                     YFRARSLLREQAVSQLENLITNQVNVVGQNVLSKEAILKEKLAETETKSLVNAVLVEN
                     PQSSQLSSIRNNLISSIAEETTSGGTNSFDDFLIVDKDGIVRLATNTKWQGAAVDPAL
                     FNFEETQSKLLQGVVPLIENEPILITSVNVTDGVFVGITSGKALQDLLQPLNGLAPYA
                     TAYFIMPGDQILSADPNNGALSPITVSSTATAQLQSNLNELKNDSAEASRTLDIVLTE
                     GEKVLAQTKWVPSIQSGVALTVKSEVVFANINSLMPFTILLVLGSIFAMGVFLYYGTS
                     RAIKPLQSLSEMARKFAEGDRSLRADSINADNEIGQLSRSFNYLADQIEDHYTSLEKK
                     VEERTLQIRTAAEVAQSVTTIPNLDEMLRKTAELLVQQFNFYQTSVFLVDRSGKNIDF
                     KTGYGSGTKDLTERKYRVQVGSSSILGWVAANNKVRIASDTIEDELHLQNEFLPGTRT
                     EACVPISINDQVLGVLDVQSTQPSAFSADAILMLQTLASQLATAIQTAGLVDSSQVNF
                     QELDRLYRSSRLIEAAKDEAEILRVGGQILKPAPYPIVFLQLNSDSIRIINVSDMLRN
                     DLSTELTQNLQANIQELNSLLMRGSVIVSGTSVELSKPVKDILRAFGLLNAVAIPVKG
                     QEALKAIVLVGSQGEIPTGSSVQPYENLADLMSVALQRASAFVQTERHLQEVETLASM
                     NELIASTSDIQDFFHALHGKIQQIIGDYSLIVAIYDEPTNSISIPFSYEAGKIQAIPT
                     FPLGEGLTSILIRTRQPLLLVKDTERRAAELGAKIVGKPAKSWMGAPLIVQNTVTGAL
                     IIQDVEHEGSFNEDDLKFFTTIASQVAGVIKNVQLLDESKQKALQVETAAEIARDISG
                     SLNLDELLSKAVNLIRDRFNFYHAGIFLLDIPGEFAIIREATGEAGAQMKRQGYKIGV
                     GSKSIVGYVTGKGETLVVSDTTKDITYYPNPLLPETRAEAAFPLRVGERILGALDVQS
                     IQPYGFHEDNLRSLQILADQLAIAVANTELFAETQEHLSQHRLLHHITSSAASGTTLE
                     EALESAVSGLQVTLGGDRVIILLVDREKRIL"
     gene            complement(3381..3839)
                     /locus_tag="PLR93_00170"
     CDS             complement(3381..3839)
                     /locus_tag="PLR93_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25398.1"
                     /translation="MSDQDQTPAVSDTQPIRKPTIPKDAAVLVVEDNVSNFVLIARML
                     GYLGIHCEWKTSGYEVVEYADTLPRLDLILMDIRLPYEDGYGALKKIRASERFKSVPI
                     IAVTAEASQEQMKKAKESGFDGFLGKPLDPDRFPDQIRRILNGEPVWEFS"
     gene            complement(3832..4224)
                     /locus_tag="PLR93_00175"
     CDS             complement(3832..4224)
                     /locus_tag="PLR93_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25399.1"
                     /translation="MPENGRGTILYVEDNPDNRNLIRRVLNAEGYAVVEAAQATQAFA
                     KLESEHIDLILMDINMPDMDGYTLTAKIKKMDKYKNIPIVAVTANVMRGDREKSLEAG
                     CDGYIQKPIDIDTLAQQIERFMTRRTNV"
     gene            complement(4365..4435)
                     /locus_tag="PLR93_00180"
     tRNA            complement(4365..4435)
                     /locus_tag="PLR93_00180"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4401..4403),aa:Gly,seq:ccc)
     gene            complement(4513..5550)
                     /locus_tag="PLR93_00185"
     CDS             complement(4513..5550)
                     /locus_tag="PLR93_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH25400.1"
                     /translation="MTSNLQQSMLDAIELELKRQVGRLDQKRTAIFHEMLTYHMGWTG
                     EGAGSQATGKRIRPLITLLSVASINNKEEKKNDSTYWLHAKSMAAAVELVHNFSLVHD
                     DIQDNSELRRGRKTVWTKWGPPMAINVGDALFVIANQAVVDLKEHYPAEMVVRAAEIL
                     NECCLDLTRGQYLDMSYEERNDLSMDDYWPMIGGKTSALLSACTQVGALLGYANEEKI
                     ELYRQFGYNLGLAFQVQDDILGIWGDEAVTGKSAASDLVEGKNSLPVLYALEKNGEFA
                     KRWKQGPIKQDEVSQVAALLEKEGGKAYAEKMSEELTQKALEYLRQADPQGEAGEAIL
                     GLANMLLKRKQ"
     gene            complement(5547..6170)
                     /locus_tag="PLR93_00190"
     CDS             complement(5547..6170)
                     /locus_tag="PLR93_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNH25401.1"
                     /translation="MKQNITAIIFDLGNVLIGWDAHRLYDRFFPTPAAVDRFLEEIRF
                     SEWNAKQDAGRPFHEGVAELSKEFPHYAELIQTYDTHWEESLTGVYEGTVEIVRELKQ
                     AGWALYLLSNFSVEKFDLIKHQHDFLNLFDDQVISGEHRTVKPEKAIFDITLKRINRK
                     AEECLFIDDSLPNIETARKMGFQTIHFQSPPLLREELKKLSIKGIDS"
     gene            6250..6672
                     /locus_tag="PLR93_00195"
     CDS             6250..6672
                     /locus_tag="PLR93_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25402.1"
                     /translation="MSRLINPDSVGKERTRLTKSIVLCIRELAKQSQVTPETKDQAAY
                     IALALQAISEGIEASVAAWEKRDYWVKADKFRMEWMWAGQYAAKLKEAVLTDDWATIA
                     TMLPSIAQKFNKIEIAQNHRLGTPWTNAYKLLALHQKL"
     gene            complement(6728..7222)
                     /locus_tag="PLR93_00200"
     CDS             complement(6728..7222)
                     /locus_tag="PLR93_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNH25403.1"
                     /translation="MTTSTTTMDKLTIKTENKFRTLTTKQSYKKGEIISTIPSEDVRD
                     RPNRYTVQIGHDKHTHVGKLAALNHSCDPNVILDTENMEMVAARDIEKGEELFFFYPS
                     TEWQMDAPFICLCGSANCINVVAGARFLPLSTLERHYLNPHIREMMVELLNNTKNNLQ
                     KIKA"
     gene            complement(7345..9708)
                     /locus_tag="PLR93_00205"
     CDS             complement(7345..9708)
                     /locus_tag="PLR93_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNH25404.1"
                     /translation="MQRLTRQLVIADVLACVIVIAALKIILTGISASVPNTVNNQYFF
                     VIGLVATCIGFGLSKAQRNSIQASAGIAALGILLVWIIGARLPQPLFDLVSAIFNTLK
                     QVILSIQHDTAVETTAIQSAWAVIASASVTLWSRSQTWLAGFTSKTVNDALMRSLAWI
                     LLYWVISAWLGWYTNKRNALIAFLPALFLLTALTAYSEKKIDSVLALLASLLLLMGIW
                     HYKSHMALWMKRRLDFSESLPLDVGQAIIAITVAVVLLAALTPSLSWDDLVNLIRRNN
                     TDSVAKMLGVRPPATSNGSGTTQPPSMPREHLLTGGFANSEKVVMTIRTGELPPLSVE
                     TLPMPVPRYYWRSQVYDQYLGSGWGSSVILKQKVSANTPLLPAFLNNYRPLHLDVQMT
                     EPEGKLFWSGILFRTDVPFTAQWRLRPTSDLFANQTVLMQSDIFIATSKATTYRAEVY
                     LPTPTISQLHSATTDYPEEIRERYLELPSELPKRVRDLALDITAGVSNPYEKAKVIES
                     YLRSNYPYDLNVPAPPTDQDATDYFLFELKRGYCDYYATAMVVLARANGLPARFVSGY
                     SPGSYDASNAQYIIRELNAHSWAEVYFPGIGWVEFEPTASLPEIERSESSTTQSEEAL
                     LQDIAPQEPKTKPLAVSWIEKKLIWVSPFAGILAIALIYFMFIERWFYLRLEPVTGIH
                     RIYQNFYRIGKIFTGQISQAETSSEFLTNIIRTLEDIETIKNNTKRLNQTKKNAEALT
                     SIYNRSLFTNYRAKKTDLVLALNLWTSLRRHLFVIKIFLLLNSKRSS"
     gene            complement(9708..11057)
                     /locus_tag="PLR93_00210"
     CDS             complement(9708..11057)
                     /locus_tag="PLR93_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNH25405.1"
                     /translation="MLLPKRTTQRLRLPTPSYNPHLKSTLRLNAKAFPVIAIIALILQ
                     IITPSRVWVTLWIVLGGVWLLCWWWVKGLSRSLEFKRELRFGWAQVGDKLEERFTINN
                     PFILPVIWVAVDDHSTLPDHYASLVISVDGRSSFQWKLNSQCTHRGVFTLGSTTLETG
                     DPFGIYTLKFEDPSSSTVVVMPPVISLPRFNILASGWAGDGRPSPRALEETTNISHTR
                     EMVSGDSLRLVHWKTSARKNKLHVREFDGTQAGNWWILLDLHKDSQVGTGWDSTEEHA
                     VILAASLIVQGLNEEHPVGLAINGHEPTWHIPRRNEYQSHALLKALAIAKPSEHPLRE
                     FIQRNSKSLGRRSSLLIITASHDPTWVEDLIPLMRRGILPTIFLFDVNTFGAQADSRS
                     LSSTLAELGVPCHIIPKDLVGSQQIRRGHEGEWKWRVTGTGKAVAVQQPIADWRALE"
     gene            complement(11156..>11393)
                     /locus_tag="PLR93_00215"
     CDS             complement(11156..>11393)
                     /locus_tag="PLR93_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH25406.1"
                     /translation="WAQMNLTHTPVEVNRADRRELMRVPGIGQKGADAILSARRLNKL
                     RDISALKKLGIIAERAAPYVLLDGKRLASQLALF"
BASE COUNT         3029 a         2516 c         2717 g         3131 t
ORIGIN      
        1 tccaatatac gtttttcgcg atcgaccaat aagataatca cccgatcacc gcccagtgtt
       61 acttgaagtc cactgacagc gctttccaac gcttcttcta gtgtagtacc tgaagcggca
      121 gaggatgtga tgtgatgcaa tagacgatgc tgtgagagat gttcttgggt ttctgcaaac
      181 aattctgtgt ttgcaaccgc aattgcaagt tgatctgcaa gaatctgtaa acttcgcaga
      241 ttatcttcat gaaaaccata gggctgaatg ctttgaacat ctaatgcgcc caaaatacgt
      301 tcgccaactc gcaaggggaa agcggcttcg gcgcgggttt ctggaagaag tgggttgggg
      361 tagtaggtga tatcttttgt agtatcactg actaccagcg tttcgccttt tccagtgacg
      421 taccccacaa tcgattttga gccgacgccg atcttatatc cttggcgctt catttgagca
      481 cccgcttcgc cagttgcctc gcgaatgatg gcaaattcac ccggaatatc tagcaagaaa
      541 attccggcat gatagaaatt aaaccgatcg cggattaagt tgacagcctt gctcaaaagt
      601 tcgtcaaggt tcaatgagcc gctaatgtcg cgggcgattt cagctgcagt ttctacttgc
      661 aacgcttttt gcttgctctc atcgagaagc tgaacgttct tgatgactcc agctacctga
      721 cttgcaatgg tagtgaagaa cttcaggtca tcttcattaa acgagccttc atgttcaaca
      781 tcttggatga tcaatgcccc cgtgaccgtg ttctgcacga tcaatggcgc gcccatccaa
      841 gatttggctg gcttcccaac gatctttgct ccaagttctg cggctcttcg ttctgtatcc
      901 ttaaccagta ataggggttg gcgcgtacga attaatattg atgttaaccc ttcaccaagc
      961 gggaaagttg gaattgcctg gattttgcca gcttcataac taaatggaat gctgatagag
     1021 tttgtcggtt catcgtagat cgcaacgatc aaactgtaat cgccaataat ctgctgaatt
     1081 tttccatgca aagcgtggaa gaaatcctga atatcagatg tagaggcaat caattcattc
     1141 attgacgcta gtgtttcaac ctcttgtaag tgcctttctg tttgtacaaa ggcactagca
     1201 cgttgaaggg caacagacat taagtctgcg aggttttcgt atggctgcac cgaagaaccg
     1261 gtgggaattt cgccctgtga gccaaccagt acgattgctt ttaaggcttc ctgtcccttt
     1321 actgggattg caactgcatt gagaagccca aaggcgcgaa gaatgtcttt aaccggcttg
     1381 gacaattcca cgcttgtgcc agatacgatg acagaaccac gcatcaacaa tgaatttagt
     1441 tcctgaatgt tggcttgtaa attttgggtc aactcagtag aaaggtcgtt tcggagcatg
     1501 tctgaaacgt tgataatcct gatactgtca ctatttagtt gaaggaagac gatagggtag
     1561 ggggctggtt ttagaatctg cccgccgaca cgtaaaatct ctgcctcatc ttttgccgct
     1621 tcgatcaaac ggctggaacg atacaagcga tctaattcct ggaagttgac ttggctggag
     1681 tcaaccaacc ctgccgtttg aattgcagtg gcgagctgac ttgccaatgt ctgtagcatc
     1741 agaatcgcat cggcactgaa agccgacggt tgtgtacttt ggacatctaa tacgcccaat
     1801 acctgatcgt tgatcgaaat gggcacacag gcttctgtgc gtgttccggg taggaactcg
     1861 ttttgaagat gtagttcgtc ttcaattgtg tctgaggcga tgcgaacttt gttgttagca
     1921 gcaacccagc ccaaaattga ggaagagccg acttgtactc gatactttcg ttctgtaaga
     1981 tccttcgtgc cagaaccgta tccggttttg aaatctatat tttttccgct gcgatcgacc
     2041 agaaacacac ttgtttgata aaagttgaat tgttgtacaa gcagttctgc ggtcttgcgc
     2101 agcatttcat caaggttggg aatggttgta acgctttgcg ccacttcggc ggcggttcga
     2161 atttgaagcg ttcgttcttc cactttcttt tcaagagagg tgtaatggtc ttcgatctgg
     2221 tctgcaagat aattaaatga ccgcgaaagt tgaccaatct cattatctgc atttatagaa
     2281 tcagcacgca ggcttcgatc accttctgca aatttgcgag ccatttcaga aagggactgt
     2341 aaaggcttaa ttgcgcgact tgttccatag tacaagaaaa cacccatcgc aaaaatagaa
     2401 cctaaaacca ataaaatagt gaaaggcatt aagctgttga tgtttgcaaa tacaacttcc
     2461 gacttaactg tcagagcaac accgctttga atagaaggaa cccacttggt ttgtgcaaga
     2521 actttttccc cttcggttaa tacaatgtcc agagtgcgag atgcttcagc actatcattt
     2581 ttcaattcgt tcagattgct ttgaagttga gctgttgcag tgctagaaac tgtaataggg
     2641 gagagcgcac cattattcgg gtctgccgat agaatttggt cacccggcat aatgaagtaa
     2701 gctgtcgcgt aaggggcaag tccattcagt ggttgcaata gatcttgtaa ggctttgcca
     2761 ctggtaatac caacaaacac accatctgtt acattgacac ttgtaatcag aattggctca
     2821 ttctcgatga gcggtactac cccttgcaat agcttagatt gtgtttcttc aaaattgaaa
     2881 agcgcagggt caactgctgc accctgccat tttgtgtttg ttgctagacg gactatccca
     2941 tctttatcaa caatcaagaa atcatcaaaa ctattggtac ccccacttgt tgtttcttct
     3001 gcaatacttg aaatgaggtt attccgaatg ctactcagct gtgaactttg tggattttca
     3061 actaaaactg cattgactag acttttcgtt tcggtttctg ccagcttttc tttgaggatt
     3121 gcttcttttg aaagaacatt ctgcccaacg acgttaacct gattcgtaat cagattttcc
     3181 aattgagaaa cagcttgctc tctcaacaaa ctgcgggcac gaaaataagc aacgcttgcc
     3241 attaaggcaa gcggaataaa tgtaaagatc aagagcgttc taacgagagt gctggtcagg
     3301 ctgcccttga ccctaccttg ttgttttcca ttcgtagtcg gcgctgcagg gattgtcatt
     3361 tagtaaattt tacaacaaca ctaactgaat tcccagacag gttctccgtt caagatccgg
     3421 cggatttggt cagggaaacg gtcggggtca agcggcttcc ccaaaaagcc atcgaaacca
     3481 gattctttcg ccttcttcat ttgttcctgg ctggcttcag ccgtcactgc aatgatcggc
     3541 acagacttga aacgttccga agcgcggatc tttttcagtg cgccataccc gtcttcatat
     3601 ggaaggcgaa tatccataag gatcaggtcc aaacggggga gggtgtcggc atactcaaca
     3661 acttcatacc ccgaagtctt ccattcacag tgaatgccca aatagcctaa catacgggcg
     3721 atcaaaacga aatttgaaac attatcttcc actaccaata ctgccgcatc cttcggaatc
     3781 gtcggtttgc gaatgggctg agtatcagaa acagcagggg tttggtcttg gtcagacatt
     3841 ggttctcctt gtcatgaagc gttcgatttg ctgtgcaagc gtatcaatgt cgatcggttt
     3901 ttgaatataa ccgtcgcatc ctgcctcaag agatttttcc ctatcgccgc gcatcacatt
     3961 agcagtgaca gctacgatcg gaatattttt atacttatcc atcttcttga tctttgcggt
     4021 caatgtatac ccatccatat cgggcatgtt gatatccatc aagatcaaat caatatgttc
     4081 gctttcaagc ttggcaaacg cctgagtcgc ttgagcggct tccacaaccg catatccttc
     4141 agcatttaac actctgcgaa tcagattgcg gttatcagga ttatcttcaa catacaagat
     4201 cgttccgcgt ccattctctg gcatagctgg tatttctcca ggcagaattt tactttaata
     4261 tagaaggcac ccctcatgac agctacctta caacttacaa ataataaagg tacggctagt
     4321 atagcataac gaaaagccga gggaaggtct cctcggcttc tttgagcggg tggcgggaat
     4381 cgaacccgcg tattgtgctt gggaagcaca tgttctacca ctgaactaca cccgcatgaa
     4441 caacggcgcg attatacgtc acgctcaaaa cctatgtcaa gcctgacgac gcgccgcttc
     4501 attccacgct cactattgtt tgcgtttcag gagcatgttt gccaacccaa gaatagcttc
     4561 gcctgcttca ccttgtgggt ctgcctgacg caaatactca agtgctttct gcgtcaactc
     4621 ttcagacatt ttctctgcat acgctttgcc gccttctttt tctagtaaag cagctacttg
     4681 gctcacttca tcctgcttaa tcggaccttg tttccagcgt ttggcaaatt caccattctt
     4741 ttcaagcgca tacaaaacag gtagcgaatt ttttccttcc actaagtcgc tagcggcaga
     4801 tttcccggtc acagcttcgt cgccccaaat gccgagaata tcatcttgaa cctggaatgc
     4861 gagtcctaag ttatagccaa attgacggta tagctctatt ttttcttcat tcgcgtagcc
     4921 aagaagagct ccaacctggg tgcaggcaga gagtagggca gaggtctttc ctccaatcat
     4981 gggccagtaa tcatccatcg agagatcgtt gcgttcttca taagacatat ccaagtactg
     5041 accacgtgtt agatctaaac aacattcatt caaaatctcc gcagcacgaa ctaccatctc
     5101 agctggataa tgttctttaa gatcgactac agcttggttg gcaattacga acaatgcgtc
     5161 tccaacatta attgccatgg gcggacccca tttcgtccac accgttttcc gtccgcgacg
     5221 gagttctgag ttgtcttgaa tatcatcatg caccaaagag aaattatgga caagttctac
     5281 tgcagctgcc attgattttg cgtgtagcca ataagtacta tcatttttct tttcctcttt
     5341 attattaata gaagctaccg aaagaagagt aatcaaaggt cggatacgtt tccctgttgc
     5401 ttgtgaacca gcgccctcac ctgtccaacc catatgatag gtcaacatct catggaagat
     5461 ggcagtgcgt ttttggtcca accgccccac ttggcgtttg agttccagtt caatggcgtc
     5521 caacatagat tgttgaagat ttgatgtcat gaatcgattc ctttaatgga aagttttttc
     5581 agctcttccc ttaatagggg aggagactga aagtgaatgg tttgaaaccc cattttacgg
     5641 gcggtttcaa tatttggaag tgaatcgtcg atgaaaagac attcttcagc cttacggtta
     5701 atacgtttca gcgtaatgtc gaagatggct ttttctggtt tgacagttcg atgctcacca
     5761 gagatgactt gatcgtcgaa caaatttaga aaatcgtgtt gatgcttgat caggtcaaat
     5821 ttttctacgg agaagttact gagtagatat agcgcccaac ctgcttgttt taattcacga
     5881 acgatctcaa cagtcccttc gtagacaccg gtaaggcttt cttcccaatg tgtatcgtag
     5941 gtctgaatca gctcggcgta atgagggaat tctttcgata actccgcaac cccttcgtgg
     6001 aaagggcgtc cggcgtcttg tttggcgttc cactccgaga agcgaatctc ttccagaaag
     6061 cggtcaacag ctgccggggt agggaagaag cggtcgtaca ggcgatgggc atcccacccg
     6121 atgaggacgt tgcccaagtc aaagatgata gctgtgatat tttgtttcat agatgccgcg
     6181 ctattttatc cgaagaagtt aaatctggcg gtataatcgc gcggttgtaa actgacttgg
     6241 agtagaacat tgagccgact aatcaacccc gattctgtag gaaaagaacg aactcgttta
     6301 acgaagtcta ttgtgttgtg tattcgtgaa ctggcgaaac aaagccaggt aacgcctgag
     6361 acaaaagatc aggcggcgta tatcgccttg gcactgcaag ccatttcaga agggatcgaa
     6421 gcttctgtag cggcttggga gaagcgcgat tattgggtga aagccgataa gttccgcatg
     6481 gagtggatgt gggctggaca atacgctgcc aagttgaaag aggctgtatt gacagatgat
     6541 tgggcaacga ttgcgaccat gttgccaagt attgcgcaga agttcaataa gattgaaatc
     6601 gcacaaaatc atcggttggg cactccgtgg accaatgcat acaagctatt ggcactgcat
     6661 caaaagttat aaggtaatag ataaaaaaac tccgaagcct aggcttcgga gtttttttat
     6721 tgcatattta ggctttgatc ttttgaagat tgttcttggt gttgttgagt agttcaacca
     6781 tcatctcgcg gatgtgtggg ttgagatagt gacgctcgag tgtggaaagc ggtaagaagc
     6841 gtgcaccggc tactacgttg atgcagtttg ctgaaccaca taagcaaatg aagggagcat
     6901 ccatttgcca ctcagtggag ggatagaaga agaaaagttc ttctcctttc tcaatatcgc
     6961 gggcagcaac catttccata ttctcagtgt cgagaataac attggggtca caggaatgat
     7021 tcaacgcggc gagtttacca acgtgtgtgt gtttatcatg accaatttgc accgtgtaac
     7081 gattggggcg atcacgcaca tcttcactgg gaattgtgct gatgatttcc ccttttttgt
     7141 aactttgttt cgttgttaga gttctgaatt tattttcagt tttgatggtc aacttatcca
     7201 tcgttgttgt tgaggttgtc attgattatc tcctttaaat ttttaaacta atttattatt
     7261 gcatcagtat agcaataata aatttaaata caagagttat ttgtataaaa caaaaaattt
     7321 taaggtttga taaatttttt gttttcaact agaacgcttg gagtttaata acaaaaatat
     7381 ttttataaca aacagatgac gtcgcagcga agtccataaa ttcaaagcta agaccaaatc
     7441 tgttttttta gcacgatagt ttgtaaacaa agagcggttg tatatagacg ttaatgcttc
     7501 tgcattcttt tttgtttgat tgagtctttt tgtattattc ttgatagtct cgatatcttc
     7561 cagtgtgcga atgatattgg taaggaattc actggaggtc tccgcttgag agatttgacc
     7621 ggtaaaaatc ttgccgatgc gataaaagtt ttgatagata cgatgaatac cagtgacagg
     7681 ttcaaggcgt agatagaacc atctttcaat gaacatgaaa tagataagtg ctattgcaag
     7741 gatccccgcg aatggtgaaa cccaaattag ctttttttct atccaagaaa ctgccagagg
     7801 ctttgtttta ggctcttgcg gagcgatatc ttgtaataga gcttcttccg attgagttgt
     7861 ggatgattca gagcgttcaa tctcggggag ggaagcggtt ggttcaaatt cgacccagcc
     7921 aatgccggga aaatagactt cagcccacga atgagcattt aactcacgga ttatgtattg
     7981 agcatttgaa gcatcataag aacctgggga gtacccagaa acaaaacggg ctggtaaacc
     8041 gtttgcgcgg gcaaggacga ccatggcggt ggcgtaatag tcgcagtagc cccgtttgag
     8101 ttcaaacaag aaataatctg tggcatcttg atctgtggga ggggctggta cattgaggtc
     8161 gtatggataa ttggatctca ggtagctttc aatgactttg gctttttcat agggattgct
     8221 cacgcctgct gtgatatcca aagcaaggtc gcggacccgt tttggtaact cgcttgggag
     8281 ttcaagataa cgttcacgaa tttcttcagg gtaatctgtt gtcgcggaat gcagctgtga
     8341 aatggttggc gtcggaaggt agacttcagc gcgatacgtt gttgccttgc tggtagcaat
     8401 aaatatgtcg gactgcatta aaaccgtctg gtttgcgaac aggtcagatg tagggcggag
     8461 ccgccattga gccgtgaaag gtacatctgt acgaaagaga atcccgctcc agaataattt
     8521 tccttcgggc tcggtcattt gcacatccaa atgaagcggg cgatagttat tcaaaaaggc
     8581 gggtaataag ggtgtattgg cagaaacctt ttgtttgaga atgacactgg agccccaacc
     8641 gctgccaaga tattggtcgt agacttggct gcgccagtaa taacgcggaa cgggcatagg
     8701 caacgtttcg acactcaacg gcggaagttc tccagttcga atcgtcatga cgaccttttc
     8761 agagttggca aagccgccag tgagaagatg ttcgcgtggc atggacggtg gttgtgttgt
     8821 gccagaacca ttggatgtgg caggaggacg cacccctaac attttggcaa ccgagtcagt
     8881 attgtttctg cgtatcaaat taacaaggtc atcccatgaa agcgatgggg taagtgcagc
     8941 cagtaagaca actgcgactg tgatcgcgat gatggcttgc cctacatcca atggaaggct
     9001 ttctgaaaaa tctaagcgcc ttttcatcca taaagccata tgagatttgt agtgccaaat
     9061 tcccatgagt aacagcaaag atgccaagag ggctaataca gagtctattt tcttctcact
     9121 atatgctgtg agtgccgtta gcaaaaataa agcaggcaga aaagcgatta aggcattgcg
     9181 tttattcgta taccaaccta gccaagcaga gatgacccaa tacaacaaaa tccaagccag
     9241 actgcgcata agagcgtcgt tcaccgtttt tgaggtgaag cctgcaagcc aagtttgcga
     9301 tcgagaccat aatgtgacag aagcagaagc tataactgcc caagcggatt gaatagcagt
     9361 cgtttctact gcagtgtcgt gttgaatcga taagatgact tgcttgagtg tgttgaatat
     9421 cgcagaaaca aggtcaaata aaggttgagg gagacgcgcg ccaatgatcc aaacaaggag
     9481 gatgcccaac gccgcaatcc ccgccgaagc ttgaatgctg ttacgttgag ctttactcaa
     9541 accgaatccg atgcacgtag cgacgagacc gattacaaaa aaatattgat tgtttactgt
     9601 gttgggcaca gaggctgaaa tacccgttag tatgatcttg agcgctgcga tgacaatcac
     9661 acatgccaaa acatctgcta tgacgagctg acgtgttaag cgctgcatca ttctagcgct
     9721 ctccaatcgg caatgggctg ttgcacggca acggctttgc cagtgccagt gactcgccat
     9781 ttccattcgc cttcatgacc acgccgaatt tgctgtgacc ctaccaagtc tttagggatg
     9841 atatgacatg gcacgccgag ctcagccaac gtggacgata acgagcggga atcagcctgt
     9901 gcgccgaagg tgttcacatc gaaaagaaag atggtgggta atattccacg gcgcataagc
     9961 ggaataagat cctcgaccca tgtagggtca tgcgaggctg taatgatcaa caagctgctg
    10021 cgccgaccaa gcgatttgct gttgcgttga ataaattctc tgagtgggtg ctctgaaggt
    10081 ttggcgattg ccaacgcttt gagcaaagca tgagattgat attcatttcg gcgcggtata
    10141 tgccaggtgg gttcatgccc attgatggct aacccgacag gatgctcctc atttagacct
    10201 tgaacaatga gcgacgctgc cagaatgacg gcgtgttctt cggtagagtc ccagcctgtg
    10261 ccgacttgcg agtctttgtg caggtcgaga agaatccacc agtttccagc ttgggtgcca
    10321 tcaaattcgc ggacgtggag tttgttcttg cgggcactgg tcttccaatg cacgaggcgc
    10381 aatgaatctc ctgacaccat ttcacgagtg tgagagatat tggttgtttc ttccaatgca
    10441 cgtggactgg gtcttccatc acctgcccaa ccgctggcaa ggatgttgaa gcgtggcaga
    10501 gaaatgaccg gtggcatgac tacaacagta gatgaggaag gatcttcgaa cttgagggta
    10561 tagattccaa aagggtcgcc ggtttcaagt gttgtgctac ccagtgtgaa aactccgcga
    10621 tgagtgcatt gcgaattgag cttccattga aaagaagatc gcccgtcgac actgatgacg
    10681 agggatgcat agtgatcggg cagagtggag tggtcatcta cagcaaccca aatgaccggc
    10741 aaaatgaatg gattgttgat cgtgaagcgt tcttcgagtt tatcgccgac ttgagcccag
    10801 ccaaagcgca gttcacgttt gaattcaagt gagcgagaaa gacctttaac ccaccaccag
    10861 caaagaagcc aaacgccgcc caagacaatc cataaagtga cccacactcg tgatggtgta
    10921 atgatctgaa gtatgagtgc gatgatggca atgacaggga acgcctttgc attcaatcgc
    10981 aaagtagatt tcaaatgcgg gttatacgat ggagtgggaa ggcgtaggcg ttgcgtcgtt
    11041 cttttaggaa gtaacatagc gacccagacg gcaaaataaa gatgcggttg cagtaaggca
    11101 accgtggaac aaactttata tatcgattat acgacggggt agggggtgtt gaactttaaa
    11161 acaaagcgag ctgacttgct aagcgttttc catctagcaa aacataggga gcggcacgct
    11221 ccgcgatgat gccaagcttt ttcagggcag agatatcacg cagtttgttg agcctgcgag
    11281 cggagaggat ggcgtcggca cctttttgac cgatgcccgg cacacgcatc aattcgcggc
    11341 ggtcggcgcg gttgacttcg actggagtgt gagtcaagtt catttgcgcc cac
//
LOCUS       DAOQFT010000005        11100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_2727_length_11100_cov_6.937619, whole genome shotgun sequence.
ACCESSION   DAOQFT010000005 DAOQFT010000000
VERSION     DAOQFT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2727_length_11100_cov_6.937619"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1193
                     /gene="mazG"
                     /locus_tag="PLR93_00220"
     CDS             108..1193
                     /gene="mazG"
                     /locus_tag="PLR93_00220"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNH25407.1"
                     /translation="MDFNQINSIFETLRLTPPSKLTLLEAQEFSLKHYPATPPDVDTL
                     ILNIDSAEMAEQVKFVLLAAYAGSHKVSAVGKAGVTETSLAEFGGAGASYPVSLFVPS
                     FGVGTSFEAFAEIVAHLRAPDGCPWDKEQTHLTLRKHLMEESYETLSAMDANDIDGMR
                     EEFGDLLLQIVLNSYIAYQDKEFTFTEVTKNVYDKIVRRHPHVFGDVKVDGVDGVLTN
                     WEKLKEKEREGKKEDKGILDGVPAALPALNQAQEYQDRAARVGFDWPEIEDVLDKVRE
                     EIEEVKTAESPEQVKAELGDLFFVLVNLSRWREVDAESALREANLKFKRRFAHIEKSA
                     KGQGRNLSDMTLEEMDAFWNEAKRLGL"
     gene            1295..2326
                     /locus_tag="PLR93_00225"
     CDS             1295..2326
                     /locus_tag="PLR93_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNH25408.1"
                     /translation="MNINFAMWRKASWQLIKMDDKKEWDALDVVSKWLIATRSAVTMV
                     TVYSCIIAGILAARDGKFDWLTFIIVTLGLFIAHGTNNILNDYTDFNRGVDRNNYFRT
                     EYGVHPLVQKFWDNKTSIRWFIVSGILATSAGVYALFHTGFSTTTIGLFAFGAVVLLA
                     YTYPFKYWGIGEFMIFLIWGPVLIGGVYFVLSGNWSWDVFVAGIPFGLSVASINIAKH
                     IDKHDPDKEKGVGTFPVRVGEANARRVNQVAIVLIYLVTLYLVLDGYFTPVMLLIFLA
                     YKEALMAIAVMNKPKPAQAPEGWPAWPVWFSGFAFQHNRKFGGLLIVALILDALIHYF
                     APTLWTGLF"
     gene            complement(2372..>2635)
                     /locus_tag="PLR93_00230"
     CDS             complement(2372..>2635)
                     /locus_tag="PLR93_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNH25409.1"
                     /translation="AEDSSALRVDVTQWHGYRLEWRSNRVAWYVDEAQVFEASVSPNP
                     PLGLVIWIDNQYAAFKPEGKISFGVLEGNEEWLEIEDLDMSSK"
     assembly_gap    2636..2735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2902..3789)
                     /locus_tag="PLR93_00235"
     CDS             complement(2902..3789)
                     /locus_tag="PLR93_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH25410.1"
                     /translation="MPKSKVIPYLEALFAVLVWGGTFIATKIALSEVSPATIVWIRFG
                     MGTVILGIVVVARKQFALPEKNEWLYFAMLGFLGVTFHQILQATGLQTAKATTTAWIV
                     ATTPVFIAILGWVALKEKLNGLQILGIVLAGFGVLLIVSKGNIGALFTGEEGTFGDFL
                     VLISSVNWAVYTILSRRELARHPAARMMFFVMLIGWLLVNVWIFGFGPGVSEIPQLTS
                     SGWVAILILGIFGSGLAYIAFYDALQEIPASQLGAFFNVEPLVTTILASFMINEAITA
                     ITLIGGAIIILGIWLVNRK"
     gene            4020..4667
                     /locus_tag="PLR93_00240"
     CDS             4020..4667
                     /locus_tag="PLR93_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH25411.1"
                     /translation="MEITWYGHSCFRLTERNYATVVTDPFDHKAVGYSALKLKADIVT
                     VSHDAPGHANLEAVKGSEHVIDGAGEFEIGGVFITAVQTAGGGKKSKDKTRNTLYVFD
                     YEGITVAHLGDLQEVPTQSEVEALGTVNILLIPVGGGSSLNAAKAAEVVSVIEPNLVI
                     PMHYSTDAAKIKLDPLNKFLKEMGLGKVESLPSLKATRSSLPQETKVVVLDYQQG"
     gene            4669..4971
                     /locus_tag="PLR93_00245"
     CDS             4669..4971
                     /locus_tag="PLR93_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25412.1"
                     /translation="MAVKVIMTWDIAAERDQEYFEFIIGEFVPGVQRLGLQPAEAWAT
                     LYGSYPQIQVGLLATDASEARRVLASAEWAMLQDRLFGYVKNYSYKVVPVRTGFQF"
     gene            5034..7445
                     /locus_tag="PLR93_00250"
     CDS             5034..7445
                     /locus_tag="PLR93_00250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNH25413.1"
                     /translation="MPNKFSKILPWFFFAAAFQSLLAVAALLRIPSEGLSLARLALLG
                     AIGGIFLLSIGLGLYARRNLLRFEWLSTTPVIISSALLSLTFSLLLFLLRYFNPERFL
                     PYYERMSPLLWLLFLLAAEAALCFLLLKNGFHPQSLTNYSSAFRASVIPLLVLFTIFL
                     FVAITKIGVTPDTGYWGEPGVAIQGWHFVTALLLGCTVLILSSSNSSLFIRYLPFVLY
                     AIAAILWLSVPLETLANSFYVSTTPPTNMPLPYSDAGFYDFSAQSLLIGTGYFGGIPP
                     RPLYVVFLSILHLVFGQNYPAVINAQVLVLAFFPVALYFLAKKLHSPAAGVTVALFAI
                     FREYTGLWIASNTRVANSKVFTTDFPTAFAIVIICLVILWWLERRDLRSTLIAGGAFG
                     IFLLFRSQSMLTIPFLFLLVLLVFNFKWSEWIKSGIIFAVTMLLAVIPWLTHNYTIAG
                     KFSFDDPNQVAVIYSQYAFTGNLDLSQFNPETDSVRERIISFTLENPAYVANFITSHF
                     LNTEIGGLLALPLIKPFNGLQEPVNLYWIEWDGNLEWYNFILVLFYLAMVALGVGSAW
                     HRMKWMALVPLAFNLGYAVSNGIARFSSWRYNMPIDWVFYFYFALGIVEVFSILAQLF
                     GAQTKETQTETPIFKPIQLSDFRVQYLLPVVVFIFIGSLPWLAKELVQPRYTSTQDEL
                     VAKLVKAGYDQNEIDIFLQQPKAILNEGRLLFPRMYWRGEGLASANPWPAYAVQDFPR
                     IGFILINANSQNLIYPTKEVLDFPQGADSIILACAGDNDLLYVRVIAFDDATYQNTPL
                     TEPCP"
     gene            7445..8482
                     /gene="mutY"
                     /locus_tag="PLR93_00255"
     CDS             7445..8482
                     /gene="mutY"
                     /locus_tag="PLR93_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNH25414.1"
                     /translation="MKNRLATQLLNWYHAQKRILPWRDHPDPYAVWVSEIMLQQTRVD
                     TVIPYFEKWMKLFPTVTALASASEQEVLNAWEGLGYYSRARNLHKAAKVVVEKFNGEL
                     PRDLEDLRSLPGIGRYTVGAIASMAFGMDEPTLDGNLRRVFSRLFDVDEFADLPVGEK
                     ILWELAAQNLPEGEAGDYNQALMDLGATICLPKNPRCLLCPLMDLCKARENGTQELRP
                     VLKPKKAVPSYVHVAAVIIERGRVLLSQRPAEGLLGGMWEFPNARIEGDPAKALVKGL
                     KAATQIQVKKKEALQVVEHAYSHFKVTVYPFVCENVSIPKNKNLKWVKLTELEEYPMG
                     KVDRQIARQIK"
     gene            complement(8508..10151)
                     /locus_tag="PLR93_00260"
     CDS             complement(8508..10151)
                     /locus_tag="PLR93_00260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNH25415.1"
                     /translation="MSKKNKLRIIPLGGLGEVGKNMMAYEYGNDIIVVDAGIMFPKND
                     MLGVDYIIPDYEYLKNKSNRILGLFITHGHEDHIGAIQHFIEDIPVPVYATPLTRGLI
                     EAKLHRNGAQHKAQLKTINAGDATRVGPFTLEYFHVNHSIPDAVGIGITTPAGLVVHM
                     SDFKFDQTPVDGWPTDFAKLAEFASRGVDLLLSDSTNAERPGWTPSEAVIQPAFEKVF
                     AEAKGRVIVATFASLISRVQQVADAAVKYGRKMALAGPSMVDNVKIARKMKYLEIPDE
                     IIVPIEQALQMQNHKVVIMCTGSQGEPSSIVGRLSAGTNRQFDLKEGDTVVLSSHPIP
                     GNEETISKTINRLLRRGAKVIHDGIAPIHVSGHAAQEEQKLLINIVRPKHFMPIHGEL
                     RQLMRHAELAQQLGVDPENTIVAENGQVIELVGKKILRGERIPGDYVFVTGESIGDID
                     HDVMRERSQLARNGIMLIDISIDKKTSRLLHEPEIITRGFVSPEDAETLLPDVRKLVL
                     RMVHDDGFDDEKDIINAVKSYLHQQTKRRPMVFVTLSKS"
     gene            complement(10457..10783)
                     /locus_tag="PLR93_00265"
     CDS             complement(10457..10783)
                     /locus_tag="PLR93_00265"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HNH25416.1"
                     /translation="MSFEKAVETIIREAQERGDFDNLKGKGQPLDLDAYFETPEDLRL
                     AYSALKNAGLVSPEVELLQEIAALKERLTTTYEEAQRNKIKKIIHDKQLEFNILLERQ
                     KRHRKG"
     gene            10854..>11100
                     /locus_tag="PLR93_00270"
     CDS             10854..>11100
                     /locus_tag="PLR93_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH25417.1"
                     /translation="MDIDFDLYRYEVRISSNPLVRLSAIDVSPSSPKHTIVFIHGFGG
                     KAEQWQYQMQKFAMDNRVIALDMRGHGLSDKPSTGYDM"
BASE COUNT         2624 a         2613 c         2739 g         3024 t
ORIGIN      
        1 agcgttagaa ttttctgggt ttatcccaac tgataatccg taatgcgtaa tccgtgcaaa
       61 cgacgagtta cgaattacgg attacgcatt taacaatagg atgttttatg gacttcaatc
      121 aaatcaactc catttttgag acgctgcgcc tcacaccgcc ctcgaaattg actctgcttg
      181 aggcacagga attttcgctc aagcattatc ctgccacacc gcccgatgtg gacaccttga
      241 tcctgaacat cgactcggcg gaaatggcag agcaggtgaa atttgttctc ctagcggctt
      301 atgcgggtag ccataaagtg tcagcggtgg ggaaagctgg agtaaccgaa acaagcttgg
      361 ctgagtttgg aggcgcgggg gcatcctatc ccgtttcgct ttttgtccca tcttttgggg
      421 tgggaacatc ctttgaagcc tttgcagaga tcgttgctca tcttcgtgcg cccgatggtt
      481 gcccgtggga taaagagcag acgcacttaa ccttgcgtaa acatttgatg gaagagtctt
      541 atgagacttt gtctgcgatg gatgcgaatg atattgacgg catgcgagag gagtttggcg
      601 atctgctttt gcagattgtc ttgaattctt atattgctta tcaagacaaa gaattcactt
      661 tcaccgaagt gaccaagaat gtttacgaca agatcgtgcg aagacaccca catgtgtttg
      721 gcgatgtgaa ggtcgatggg gtggatgggg tgcttaccaa ttgggaaaaa ttgaaggaaa
      781 aagagcgcga aggcaagaag gaagataaag gcattctgga tggtgttccc gctgccttgc
      841 ctgctttgaa tcaggcacag gaatatcaag accgtgcggc acgggttggc tttgattggc
      901 ccgaaattga ggacgtgctg gataaggtcc gtgaagagat cgaggaagta aaaactgccg
      961 agagcccgga gcaggtcaaa gcggagctgg gtgatttgtt ctttgtgctt gtgaatttat
     1021 cacgctggcg tgaagtggat gctgaatccg cgctgcgtga ggcgaacctg aaatttaagc
     1081 gccgttttgc ccatattgag aaaagcgcca aaggacaggg gcgaaacctt tcagatatga
     1141 cccttgaaga gatggacgcc ttctggaacg aggcaaaacg cctgggactc taaacctgtg
     1201 caaaagttcc ccttttcaca aaagagtcgt ataatgcgtt ttcgattggt caattaacgg
     1261 tgcaagccgt ttcaatatct caaggagaga gtatatgaat atcaattttg ctatgtggcg
     1321 taaagcctcc tggcagttga tcaagatgga cgacaagaaa gagtgggacg cgctcgatgt
     1381 tgtatccaaa tggttaattg ccacccgttc tgctgtgacc atggtcacgg tttattcctg
     1441 tatcattgct gggatcttgg cggcgcgtga tggaaagttc gactggctga cctttatcat
     1501 tgtcaccctc ggcttattca ttgcacacgg caccaacaac atcctcaatg attacaccga
     1561 cttcaatcgc ggcgttgaca ggaacaatta tttccgcacc gaatatggtg ttcaccccct
     1621 cgttcaaaaa ttctgggata acaagacctc cattcgctgg ttcattgtga gtggcattct
     1681 tgccacttcg gcaggtgttt atgcactctt ccacactggc tttagcacta ccaccattgg
     1741 tttgtttgcc ttcggtgctg tggttttgct ggcgtatacc tatcccttca agtattgggg
     1801 cattggcgaa ttcatgatct tcctcatttg gggaccggtc ttgattggtg gcgtgtactt
     1861 tgtgctttct ggcaattgga gctgggatgt gtttgtggct ggtattccct ttggtttgag
     1921 cgttgccagc atcaacattg ccaagcacat cgataagcat gaccctgata aagaaaaagg
     1981 cgtgggaaca ttccctgtcc gcgtgggtga ggcgaatgcc cgccgcgtga atcaggtcgc
     2041 cattgttttg atctacttgg ttactttgta cctggtgctc gatggctatt tcacccctgt
     2101 aatgctcttg atcttccttg cttataaaga agccctcatg gcaattgccg tgatgaataa
     2161 gcccaagcct gctcaggcgc ctgaaggctg gcccgcctgg cccgtgtggt tctctgggtt
     2221 tgctttccag cacaaccgca agtttggtgg tttgctcatc gtcgctctta ttctcgatgc
     2281 gctgatccac tactttgcgc ccaccctgtg gaccggtttg ttctaaagca taaatgataa
     2341 aaaaataggc tccaattttg gagcctattt tttatttgct actcatgtct aaatcttcaa
     2401 tttctagcca ttcttcgttg ccttctaaaa ccccaaatga aattttgcct tcaggcttaa
     2461 atgccgcata ctgattatcg atccagatga ccaaccctaa gggcggattc ggacttactg
     2521 acgcctcgaa cacttgggct tcatctacat accaagctac ccggttcgat ctccactcga
     2581 gcctgtatcc atgccattga gtgacatcca ccctcagcgc ggacgagtcc tcggcnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtca gagcatgatg cgggaatttt
     2761 ttccgaggta gaccaaagta atcatcgatc tgtgcaaaac gataggcaga cgaatctccg
     2821 gctgggattt tcatcaaata gcctttttcg gtctttgtga tgctagcttc aggggtagtg
     2881 cgtgctttta atggcatttg attatttccg atttaccaac caaataccta atatgataat
     2941 tgcgccgccg atgagagtga ttgccgtgat ggcttcgttg atcatgaatg aagccagaat
     3001 ggtagtgacc aacggttcaa cattgaagaa tgccccaagt tgtgaggcgg gaatttcttg
     3061 caaggcatca tagaatgcaa tgtacgccaa ccctgaaccg aagataccaa gaatcagaat
     3121 ggcaacccag cccgaagacg tgagctgcgg aatttcactc acacccggac caaaaccaaa
     3181 aatccacaca ttgaccaaaa gccagccgat cagcatgacg aagaacatca tccgtgccgc
     3241 agggtgacgt gccaactcac ggcgcgagag gatggtgtac accgcccagt tgaccgagct
     3301 gatgaggacg aggaaatcac cgaaggtccc ttcttcgcca gtgaaaagcg caccgatatt
     3361 gcccttactg acgatgagca aaaccccaaa cccggcaaga acaataccga gaatttgcaa
     3421 cccgttcagt ttttctttga gtgctaccca accgagaatg gcaatgaaaa cgggcgtggt
     3481 tgccacgatc catgcggtgg tggtggcttt ggcagtttgt agacccgtag cttgtagtat
     3541 ctgatgaaag gtgactccta gaaaaccgag catggcaaaa tatagccact cgtttttttc
     3601 aggcagggca aattgcttgc gagcgacaac gacaataccg aggatgactg ttcccatccc
     3661 aaaacggatc cacacgatgg tagcagggga gacttcgctg agcgcgatct tggtggcgat
     3721 aaatgtccca ccccaaacga ggacggcaaa taaagcttcg agatagggaa tgactttaga
     3781 ttttggcata aggtttcctg tggaaagagt tggagaatta taattgttgt ttactacact
     3841 actttaaggg gtatttttcc taagcggaat tcaccatttg ggtagggatt ttgaaggtgt
     3901 tgtgatggtc gggaaatagt cccaccaaaa actaagacct atttgactat tagacgacca
     3961 aacttcattt tgtccgtgta cccatcgggt ataatatctc cctcggaggc actagctaca
     4021 tggaaattac atggtatgga cattcttgtt ttagactgac agaacgcaat tacgcgactg
     4081 ttgtcaccga cccgttcgat cacaaggcgg tgggctacag tgcgctcaaa ctaaaagcgg
     4141 atattgtcac cgtcagccac gatgcacccg gtcacgccaa tttagaggca gtgaagggca
     4201 gtgagcatgt catcgacggc gcgggcgaat ttgagatcgg cggtgtattt attacagcgg
     4261 ttcaaactgc cggaggcggc aagaaaagta aggataagac ccgcaacacc ctctatgtgt
     4321 tcgattacga aggtatcact gtggcacacc tcggcgactt gcaggaagtg cccactcaaa
     4381 gcgaagtaga agccttggga acagtgaaca ttctcctcat cccagttgga ggcggaagca
     4441 gtctcaatgc cgccaaagct gcggaagtgg tcagcgttat cgagccgaat cttgtcatcc
     4501 ccatgcatta ctccaccgac gccgccaaga tcaagctcga cccattgaac aaatttctca
     4561 aagaaatggg tctcggcaag gttgagtctc tgccttcttt gaaagccacc cgttcttcat
     4621 taccgcaaga aaccaaggtg gttgtgctcg attaccaaca aggatagtat ggcagtcaaa
     4681 gtcatcatga cctgggatat cgctgctgaa cgcgaccagg aatattttga attcattatc
     4741 ggtgaattcg tgcccggtgt gcaacggctt ggcttacagc ctgccgaggc ttgggcaacc
     4801 ctttacggtt catacccgca aattcaagta ggcttgctcg ctaccgatgc gtctgaagcg
     4861 cgccgtgtgt tggcatctgc tgagtgggcg atgttgcaag accgtttgtt cggctatgtt
     4921 aaaaactatt cttataaagt tgtacctgta agaactgggt ttcaatttta gataccaata
     4981 caccatcatt gcgaggaggg tgatagctct cccgcaatga cggaataggt cacatgccaa
     5041 ataaattttc aaaaattctg ccgtggtttt tctttgccgc ggcatttcag tctctactcg
     5101 ccgtcgctgc actgttgcga attccatctg aaggcttgtc gctggcgcgc cttgccttgc
     5161 ttggagcaat aggaggcatc tttttattaa gcataggatt gggactgtat gcacgccgca
     5221 accttttacg cttcgaatgg ctttcgacaa caccggttat tatctcgtcc gcgcttctct
     5281 cgctgacctt tagcctgctt ctgttccttt tacgttattt caaccctgag cgttttctac
     5341 cctactacga gcggatgagt ccgctgcttt ggcttttatt cttgcttgcc gctgaagccg
     5401 ctctctgctt ccttctcctc aaaaacggat tccatccgca aagcctcaca aactatagct
     5461 cggcatttcg cgcttctgtt attccgcttt tagtcctttt tactatcttt cttttcgtag
     5521 ctattaccaa gatcggcgtc accccagata ccggctactg gggcgaacct ggtgtagcca
     5581 ttcaaggctg gcattttgtt actgctcttc tgttgggatg cactgttcta atactttcct
     5641 cctctaactc atcgcttttt attcgttact tgccttttgt tctttatgct attgctgcca
     5701 tcctttggtt aagtgttccg cttgaaacgt tggcaaatag cttttatgtc tccacaacgc
     5761 caccgacgaa tatgccttta ccatattccg atgctggttt ttacgacttt tctgctcaaa
     5821 gcttgttaat tggtactggt tacttcggtg ggattccgcc acgcccattg tatgtggttt
     5881 ttctatccat cttgcatttg gtatttgggc aaaattatcc agccgtgatc aatgcacaag
     5941 ttttggtatt agcatttttc ccggtcgctt tatattttct tgcgaagaaa ttgcactctc
     6001 ccgccgccgg tgtgaccgtt gccctctttg ccattttccg cgaatacacc ggtttgtgga
     6061 ttgcctcgaa tacccgtgtc gctaactcca aagtctttac cacagacttc ccaacagctt
     6121 ttgctattgt cattatctgt ttggtcattc tttggtggct ggagcgaaga gacttgcgtt
     6181 ctaccttaat tgcgggtgga gcatttggca ttttcttgct cttccgttcg caatctatgc
     6241 taaccattcc atttctgttt cttttggtct tgttggtatt caacttcaaa tggagtgagt
     6301 ggattaaatc gggcattatc tttgctgtca ccatgttatt ggctgtcatt ccatggctga
     6361 cgcataacta caccatcgca ggcaaattct catttgatga cccgaaccag gttgcggtga
     6421 tctatagtca atatgctttt acaggcaacc ttgacctcag ccaattcaac cctgagaccg
     6481 atagcgtccg cgaacggatt atttcattca cacttgaaaa cccggcgtac gtggcgaact
     6541 ttattacctc tcatttcctg aatactgaga ttggcggttt gcttgcgctt ccacttatca
     6601 aaccattcaa tggactgcaa gaaccggtca acttgtactg gatcgaatgg gatgggaacc
     6661 ttgaatggta caacttcatt cttgtcttgt tctatctggc aatggttgca ctaggtgtag
     6721 gctctgcttg gcatcgcatg aagtggatgg cgcttgttcc tttggcgttc aatttgggat
     6781 acgcggtttc caatggcatc gcccgttttt ctagctggcg ctataacatg ccaattgact
     6841 gggtctttta cttctacttt gcccttggca ttgtagaagt gttcagtatt ttggctcagt
     6901 tatttggagc ccagacaaaa gaaacccaaa ctgaaacacc tatattcaag cctattcagt
     6961 tgagcgattt tcgtgtgcaa tatcttttgc ctgtagttgt atttattttc attggttctt
     7021 tgccttggct ggcaaaggaa ttggtgcagc cgcgttatac atcaactcaa gatgaattgg
     7081 tggcaaagct tgtaaaagca ggatatgacc agaatgagat agatatattc ctgcaacaac
     7141 ccaaagcaat tctcaatgaa ggtaggttgc tatttccgcg catgtactgg cgcggtgaag
     7201 ggcttgcttc tgcgaacccg tggcccgcat atgccgtgca agatttcccg cgtattgggt
     7261 ttattttgat caacgcaaat tctcagaatt tgatttaccc aaccaaagaa gtgttggatt
     7321 ttccccaagg tgccgatagc attatccttg catgtgccgg ggataatgat ttgttatacg
     7381 tccgcgtgat tgcttttgat gatgctactt accaaaacac tccactaacc gaaccctgtc
     7441 cctgatgaag aaccgcctcg cgactcaact cctaaactgg taccacgccc aaaagcggat
     7501 tctcccctgg cgcgaccacc ccgatccata cgcggtgtgg gtttccgaga tcatgcttca
     7561 acaaacgcgg gtggacacag tcattccata ttttgaaaaa tggatgaagt tatttcccac
     7621 cgtaacagcg cttgcttctg ccagcgagca ggaggtgctc aatgcatggg aaggcttggg
     7681 ctattacagc cgtgcgcgga atttgcacaa agccgctaaa gtggttgtag agaaattcaa
     7741 cggcgaactt ccacgtgatt tagaagattt gcgtagcctg ccggggattg gtcgctatac
     7801 tgttggggcg attgcatcca tggcatttgg catggacgaa cccacacttg atggtaattt
     7861 gcgacgggtg ttctctcgat tatttgacgt agatgaattt gcagatttac ctgtcggcga
     7921 aaaaattctc tgggagttgg cggcgcagaa tttgcccgaa ggcgaagcag gcgactacaa
     7981 ccaagccctg atggacctcg gcgcgacgat ctgcctgccg aagaatccgc gctgtttgct
     8041 ttgcccattg atggacttat gcaaagcacg tgaaaacgga acgcaggaat tgcgccctgt
     8101 attgaagccg aagaaggcag tgccatccta tgttcatgtg gcggcggtga ttatagagcg
     8161 cgggcgcgtg ttgctcagtc aacgcccggc ggagggattg ttggggggca tgtgggaatt
     8221 cccgaacgca agaatagagg gcgatccggc aaaggcgttg gtgaaaggct tgaaggcagc
     8281 gacccagata caggtcaaga aaaaagaggc gttgcaagtc gtcgaacatg cgtacagcca
     8341 ctttaaagtc acagtctatc cgtttgtgtg cgagaatgtt tccatcccca aaaataaaaa
     8401 tttgaagtgg gtgaagctga ccgagttgga agaatacccg atggggaagg tagatcggca
     8461 gattgcccgg caaataaaat gaccgcctta cgacggtcat ttttgtttta agacttactg
     8521 agtgtcacga agaccatggg tctgcgtttg gtttgctgat gtaagtaact cttcacggcg
     8581 ttgatgatgt ctttttcatc gtcgaagccg tcgtcgtgaa ccatgcgcag gacgagcttg
     8641 cgcacatcgg gcaggagggt ttcggcatct tccggcgaga cgaagccgcg ggtgatgatc
     8701 tccggctcat gcaggaggcg actggttttc ttatcgatgc tgatatcaat gagcatgatg
     8761 ccattgcggg cgagttgact gcgttcacgc atcacatcat ggtcgatatc accaatcgat
     8821 tcgcctgtga cgaagacata atctccgggg atgcgttcgc cgcgtagaat ctttttaccg
     8881 actagttcaa tgacctgacc attttctgcg acaatcgtgt tctcgggatc tacacccaat
     8941 tgctgggcaa gttcagcatg acgcatcaac tggcgtaact cgccatggat gggcatgaag
     9001 tgtttgggac gcacaatgtt gatgagcaat ttctgctctt cttgagccgc gtgcccagaa
     9061 acatggatgg gagcaatgcc atcgtgaatg acttttgcgc cgcgtctcaa cagacgattg
     9121 atggtcttgc tgatggtctc ttcgttgcca gggatggggt gtgaagaaag tacgaccgta
     9181 tcgccttctt tgagatcaaa ctggcggttg gtgcctgcgg aaagacgccc aacaatggaa
     9241 gagggttcgc cttgcgagcc agtacacatg atgaccactt tgtggttttg catctgtaat
     9301 gcctgctcga tgggaacaat gatttcatcg gggatttcaa ggtatttcat cttgcgggcg
     9361 atcttgacgt tatcaaccat gctcggaccc gctaacgcca ttttgcgtcc gtacttgacg
     9421 gcagcgtctg cgacctgttg cacgcgcgag atgagcgaag caaaggtggc aacgatcaca
     9481 cgtcctttcg cttcggcgaa gaccttttcg aaagcgggct gaatgaccgc ttcagaggga
     9541 gtccaaccgg ggcgttcggc gttggttgag tcggagagta aaaggtccac accgcggctg
     9601 gcgaactcgg cgagtttggc aaagtcggtg ggccaaccgt cgacgggggt ttgatcgaac
     9661 ttgaaatcgc tcatgtgaac gacgagtccc gccggggtgg tgatgccgat cccaacagca
     9721 tcagggatcg agtgattgac gtggaaatat tccaaggtga aaggaccaac gcgggtggcg
     9781 tcaccagcat tgatggtctt tagttgggct ttgtgttgcg ccccattgcg gtgcaatttg
     9841 gcttcgatca acccgcgggt gagcggggtg gcatagactg ggaccggaat atcttcaata
     9901 aagtgttgta tcgcgccgat gtggtcttca tgcccgtggg tgataaataa ccctaaaata
     9961 cggttggatt tgttctttaa atactcatag tctgggatga tgtaatctac gcccaacata
    10021 tcgttctttg ggaacatgat gccagcgtcg accacgatga tgtcgttgcc gtactcatac
    10081 gccatcatgt tctttcccac ttcgcccagc cccccgagcg ggataattct taatttattt
    10141 tttttactca tataattttt ctgtatacct cacagatttc aaagattaga aaccgcctat
    10201 gcggttaaag tcaaggtagg tgttgggtag gagtttcttg caagaaaggc ttgcagattc
    10261 cagtaaacgt cttttccctc aaacaaacaa aaagaccttc gctgacttgc tcgcgaagat
    10321 cgatgccggt aaaacacgat tgttctcaaa caaactgccg aattcggcag gaaaagtcaa
    10381 cttaactgtc aaagagtata gcagggcggg cgggttttgt caaagcgggc gtgttataga
    10441 atcgttaaag ctttggctac cctttgcggt gccgtttctg gcgttccagc agaatgttga
    10501 actccaactg cttgtcgtga atgatctttt tgatcttatt tcgttgggct tcttcgtacg
    10561 tagtcgtcag ccgttctttg agagcggcga tctcttgcaa tagttccact tctggcgaga
    10621 ccaagccggc attctttaat gccgaatacg ccaggcgtaa gtcttcaggg gtctcaaagt
    10681 aagcatccag gtcaagaggc tgccccttgc ctttgaggtt gtcaaagtcg ccacgttcct
    10741 gcgcttcacg aatgatggtt tccactgctt tttcgaaact catattgcgc cattatatac
    10801 cttatttcac aaagaaaatt gacaatctcc ctcgtctctt gtacacttct ctaatggaca
    10861 tagacttcga cctctaccgt tacgaagtac gcatttcctc caacccgttg gtacggctct
    10921 ccgccatcga cgtttccccc tccagcccaa aacatactat tgtctttatc catggcttcg
    10981 gcggcaaagc tgagcaatgg caatatcaaa tgcaaaagtt tgcgatggat aatcgcgtca
    11041 ttgctttgga tatgcgcgga catggactct ccgataaacc gtccacaggc tatgacatgg
//
LOCUS       DAOQFT010000006        10197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3073_length_10197_cov_5.926839, whole genome shotgun sequence.
ACCESSION   DAOQFT010000006 DAOQFT010000000
VERSION     DAOQFT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3073_length_10197_cov_5.926839"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLR93_00275"
     CDS             complement(<1..694)
                     /locus_tag="PLR93_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH25418.1"
                     /translation="MKSNLLKGLSAIVLIGLLLTACGGGAPAVTEAPAGEATSAPAEA
                     TEAPATSGGTLTLGIPVEPETLDPGDAVYVQEQFILMNLFDSLLSISPDGELHPGLAT
                     TWEPNAEYSEFTFTLREDVTFHDGTPFNAEAVKASFDHINSDAVLESGGKSLLTDHSY
                     VETVVVDDYTVTVKFGASYPLFLRDAARQWLSISSPAALEQYGSDYGRNPVGTGPFKF
                     VQWDAQSQIVIER"
     gene            complement(903..2042)
                     /locus_tag="PLR93_00280"
     CDS             complement(903..2042)
                     /locus_tag="PLR93_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNH25419.1"
                     /translation="MSFEFATANRIIFGAGKLSGLGDQLKGSVKRLLLVRGRSADAIP
                     RVREILSAQGIQFNEFEVHGEPTVDVVREGVKFADGCDAVIGLGGGSVLDAGKAIAAL
                     ATNPNDVVDYLEVVGKGQPLTEKPLTYIAIPTTAGTGTEVTRNAVLDVPEHGVKVSLR
                     SPMMLPSLAIVDPELTYSLPPEITAACGLDALTQLIEPFVSVKANPLTDAICREGMKH
                     AAKSLRRVYQNGADKEAREGMALASLFGGLALANAALGVVHGFAGPLGGMLHAPHGAI
                     CARLLPLVMEANIEAMKAEKHAGLARYEEVARILTGNAATVEDGLQWVKELVRELNIP
                     ALSAHGMTKAHIPEAVQKTLNASSYKGNPILLSPEILQKILEKAL"
     gene            complement(2045..2350)
                     /locus_tag="PLR93_00285"
     CDS             complement(2045..2350)
                     /locus_tag="PLR93_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNH25420.1"
                     /translation="MNIVLVHVHVKPEFVDAFKQASLENASNSVKEEGIARFDVIQQA
                     DDPTRFILVEVYKTVEAQSAHKETAHYLTWRDTVAEMMAEPRQGVKYSNIFPADGGW"
     gene            complement(2360..3502)
                     /locus_tag="PLR93_00290"
     CDS             complement(2360..3502)
                     /locus_tag="PLR93_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH25421.1"
                     /translation="MQTDLLIIGAGPFGLSLAAYAKSLGMDFLVAGKPMEFWKKNMPA
                     GMFLRSASDWSLDPTDRFSIMNYLETLGKTPKDVEPLSLEFYLQYAQWFQSGAGIETI
                     SEYVTRLDQKEDGFLATFEDGKVIEAKNVAIAIGMGYFKNYPAALTDILPVGRYEHTC
                     DAVQLSEYKGRRVLILGGRQSAFEWAALLNDGGAAEVHVVHRHESPKFAEADWSWVPP
                     LVDGMVADPAWFRGLPQAEKDAIVKRMWGEGRLKVEPWLEKRVMKPNTSIHQKASLLA
                     CVERSDGALDVKLDNGDAFVVDDIILATGYKVELERLSFLKNGNMFDKVEVKNGFPVL
                     DPHFQTTAKGLYFTSMPAGQDFGPFFGFTVSVRASAKIIGNALVSK"
     gene            complement(3513..4124)
                     /locus_tag="PLR93_00295"
     CDS             complement(3513..4124)
                     /locus_tag="PLR93_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNH25422.1"
                     /translation="MWVYLIQGVGYGFAAASQIGPFQTFLISQALTRGWKRALPGAFA
                     PFLSDGPIILLSVLVLSQLPDWAQRAMYIVGGLFVLYLAYGSYKSWRNFDETLAHPEL
                     SGQQTIFKAAMTNALSPGAYLFWMFVTGPILVQGWRETPINGLGLLIGFYTTFIICLI
                     AMIIVFGAASRLGPRLNRVLLGLSCIVLFGFGLYQFWLGIFVN"
     gene            complement(4131..5039)
                     /locus_tag="PLR93_00300"
     CDS             complement(4131..5039)
                     /locus_tag="PLR93_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH25423.1"
                     /translation="MKRSIKFVLGAVVGILVLGAAGILWKTRQEAYNLLHAPMADREL
                     PNELPSDYNMQYEDVTVTNPDGMKLVGWFIPSENGAVIIMQHGYKSTRSEMLNEAEMM
                     HRHGYGILLTTVRAHDESEGELITLGYYEMADLEAWYQYLISRDDIDPEKIGILGNSY
                     GGMLSIQYAAQNQNIKAVVADCAFSSMKDTVSTSVRHFTGLPDFPFVPLIVFWAENMT
                     GISIDDIDATKWIPNISPRPVFLMQGGKDTVISPTSGQILYDAAGEPKELWFDPELGH
                     VDFDSERAEEFEARVSAFFDQYLLGQ"
     gene            complement(5145..7472)
                     /locus_tag="PLR93_00305"
     CDS             complement(5145..7472)
                     /locus_tag="PLR93_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH25424.1"
                     /translation="MNEFTQNTPVVEKKHSNGALALFLIVLLPMPFCLFIYNFIVWST
                     EQSAIASSSMKQLAWAGFIGLGVQIFLMSLVFGLLWYLTSDDRFKGWYASLFAASLFG
                     IPALLLRALGPNNDQLGSILQFVLAVIVALAVIYIRKDKLVWDFSALPFGFLVAALGF
                     APLAVYGSFGSPADVVLSLFAGLAFGLLAAVLMSDAENIFLNGIGAGGLLALLASSFG
                     YDGSQLILVVLVPAFGCAIAALYPSRAAATVAAGLLAFAGLAFFDPTELTIVLGDIGG
                     LAVKAVGIALLIGWGVSVVALILRQVMGSGSTSGVKRAAGWVGAVAGWVMVIAAFFIW
                     GNPGNYSDRLFVIFKDQADISDVASIKNRDERLTEAYTRLTDHANKTQADLRETLDTF
                     GVAYTPYYLENAMEVQGGTLVRLFLMTRSEVDRVIPSPRLRAAPEDSPEPGFESAPPA
                     DYVGWNIQLIGADRVWEEFGVRGEGIVVGQSDSGVDGNHPAIEKQYRGYNGSDDYNWF
                     DPWDGTTSPNDEGGHGTHTMGTILGNNGIGVAPDAQWIGCVNLDRNLANPALYLDCMQ
                     FMLAPFPIGGDPFVDGDPTQAAHVMNNSWGCPSIEGCDPNALLYAANHLRDAGIFVVV
                     STGNDGPNCETVEAPLSLYDSVFSVGAIDQSGDLARFSSRGPVTADGSGRIKPDIVAP
                     GVDVFSSLPENSYGSNSGTSMAGPHMVGVVALIWSAQPELIGDIDATEQLIIDTAKPY
                     MGDRSLGCFSGDIPSNAFGFGVVDVYEAVKEALNK"
     gene            complement(7498..8151)
                     /locus_tag="PLR93_00310"
     CDS             complement(7498..8151)
                     /locus_tag="PLR93_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25425.1"
                     /translation="MIKPVRWNTFIRDFIRIQIGFLLYGLAITLMIRGNLGTSAWAVL
                     EVALADFLHITVGTMTVIMGFIVLILSLLMREQIGWGTLGNILSIGPWVDLCMALVPT
                     VNNNLAIQILMLLSAIIIMGLASAIYIGVDAGAGPRDSLMLAIRRKTGVSIRVSRAII
                     EVTVVVLGWLLGGPAGIGTVVHAVLIGPSVQWGFKVFKVQAHTEREEVQAGIEGGAE"
     gene            complement(8214..>10197)
                     /locus_tag="PLR93_00315"
     CDS             complement(8214..>10197)
                     /locus_tag="PLR93_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH25426.1"
                     /translation="EQLGYAIDELIGSPLLDIFYPDDREHVLQKMLACINDPEKTVQV
                     EARKQRKDKSILWVREFARAIRNTDGHLVILIVCNNVTEEVKAEEALVQSEAELRALF
                     SSMKDSVIVIDRNGIYQSVAPTNPETLYIPPAEVIGKGLSAFFPEEQVQEFLKAIEYV
                     LATGKTVIIEYQIILAEVSPWFESRVSPLGPDTTIWVARDVSERKKIQSALIQSEQAY
                     RSLFENMPIGLYRSSIHGELLDANPSLLRMFGYEDLETMKGKKAWEFYNDPALDDEFR
                     QQISENGFLNRYESEFIRQDGTRFWAEDYVRAVYDGKGTPLYYEGSLIDVTDRKAAEK
                     RLKESEKQYRLLAERIADVVWVLDLATLEFKYISSSVQNLLGHSAEELLNKHVSMVLT
                     PSAMMAIESITPERIKRFLAGDKTSVTELYQLDHVHKDGSIIVVEVSSTFVMNENATI
                     EAIGVTRNITERKKAEDDLRLANSSLQIAHKELQQLFEYEQILARTDGLTRLYNRRYF
                     FELAAREFNSAIRYQRPLTLIIFDVDGFKQANDTFGHDFGDRTLVEVSKTANLQVREV
                     DILARYGGDEFTILLPETNVEQAFLIAERIRKAVADVYIDMGGFSTFVTISMGIAELS
                     FEPQDQSIEDMIRRADQALYQAKQQGRNKVVIYSDK"
BASE COUNT         2839 a         2756 c         2161 g         2441 t
ORIGIN      
        1 tgcgttcgat cacgatctga gattgggcat cccactgcac gaacttgaac ggaccagttc
       61 ccaccggatt gcgtccatag tcactgccat attgttccaa ggcggcaggc gaagaaatgc
      121 tcaaccactg acgggcggca tcgcgcaaaa agagcggata cgaagcaccg aacttaacgg
      181 tgacggtgta atcatcgaca acgactgtct ccacataaga gtggtcagtc aacaaagact
      241 taccgccaga ttccaacacc gcatcactgt tgatgtgatc gaatgaagcc ttcaccgctt
      301 cggcattgaa gggtgtgcca tcatggaagg tcacatcttc gcgcaacgtg aaggtgaatt
      361 cggaatactc cgcattgggt tcccaggtag tagcaagacc cgggtgcaac tctccatccg
      421 gcgaaatgga aagaagggag tcgaacagat tcatcagaat gaactgttcc tgcacataga
      481 cggcatctcc ggggtcgagc gtttccggct caaccggaat gccaagcgtg agcgttccac
      541 cagaagtggc tggcgcttcg gtcgcttctg caggcgcgga tgtggcttct ccggcaggtg
      601 cttccgtaac cgcaggcgcc ccaccaccac atgcggtcaa taacagtccg atcaatacga
      661 tagcggacaa ccccttaagt aagttggatt tcatattatc tccttttata aattgcgacc
      721 ggtattataa agccaatact ttataaaact aatcctatag gaattatatc attttacact
      781 taacctaaaa aacacctcat aaaatgttta aattcctatt cgtaccaaaa tgaaaaggtt
      841 tttacctaga agaggtaaaa acctgaatat ttaccccacc caaaagagaa atatcactga
      901 tgttaaaggg ctttctctaa aattttttgc aatatctcag ggcttaacag aattggattc
      961 cccttgtagc tactggcatt caaggtcttc tgtacggctt cagggatatg cgccttagtc
     1021 ataccatgcg cagaaagggc tgggatattc agctcgcgca ccaactcctt cacccattgc
     1081 aaaccatcct ctacagtggc agcatttcct gttaagatgc gcgccacttc ttcatagcgc
     1141 gccaacccgg catgcttctc tgctttcatc gcttcgatat tcgcttccat caccaaaggc
     1201 aacagtctcg cacaaatggc tccatgcggg gcgtgtaaca tcccacccaa cggacctgca
     1261 aacccatgca cgacaccaag tgcggcattc gccaacgcca acccgccaaa cagactcgcc
     1321 aatgccatgc cttcacgcgc ttctttatcc gcaccattct gatacacacg gcgtaaagat
     1381 tttgccgcat gcttcatacc ttcacggcaa atcgcatccg taagtggatt cgctttcacc
     1441 gaaacaaaag gctcgatcaa ctgtgtcaac gcatccaaac cacaggcggc agtaatttct
     1501 ggcggcaaag aataagtcaa ttcggggtca acgatggcga ggcttggcaa catcattggg
     1561 ctgcgcaagc tgaccttcac gccatgttcc ggcacatcca gcaccgcatt gcgcgtcact
     1621 tcggtcccgg tccccgctgt ggttggaatg gcaatataag tcaacggttt ctcagtgagc
     1681 ggctgcccct tccccaccac ttccaaataa tcaacgacat cgttagggtt tgtcgccaat
     1741 gccgcgattg ccttgcctgc atccagcaca ctgccgccgc ccaaaccgat cacggcatcg
     1801 cacccatcag caaacttaac cccttcgcgc accacatcca ccgtcggttc gccatgaact
     1861 tcaaattcat taaattggat tccctgtgca gacagaatct ccctcacacg cggaatcgca
     1921 tccgctgacc tgcccctcac caacagcaaa cgcttcacac tccctttgag ctggtcgcct
     1981 aacccagaaa gcttccctgc cccgaagatg attcgatttg ctgttgcgaa ttcaaaggac
     2041 atttttacca accgccatct gctggaaaga tattgctgta tttcacgccc tgtcgtggct
     2101 ctgccatcat ctccgcgaca gtatcacgcc acgtcaagta atgagccgtc tctttgtggg
     2161 cagattgagc ttcaacggtc ttatacactt ccacaaggat aaaacgcgtc gggtcatccg
     2221 cttgttgaat gacgtcaaat cgcgcaatac cttcttcctt cacactattc gacgcatttt
     2281 caaggcttgc ctgcttgaag gcatccacaa attctggttt aacatgcaca tgcacaagaa
     2341 ctatgttcat ttgaagctcc tatttagata ccaaggcgtt tccgatgatc tttgcagaag
     2401 cccgcaccga aacagtgaaa ccaaagaacg gaccaaaatc ctgcccggct ggcatgctgg
     2461 tgaagtacaa ccccttggct gtggtttgga aatgcgggtc gagaaccggg aaaccattct
     2521 tcacttcgac cttatcgaac atattgccgt tcttcaagaa agacaatcgc tccaactcga
     2581 ccttatagcc tgtggcaaga ataatatcat ccaccacgaa tgcatcaccg ttatccagtt
     2641 tcacatccaa tgcgccatca ctgcgctcca cacaagcgag caaactggct ttctgatgga
     2701 tgctcgtgtt cggtttcatc acgcgcttct ctaaccacgg ctccaccttc aaacgcccct
     2761 caccccacat acgtttaacg atggcatctt tttccgcctg tggcagcccg cggaaccagg
     2821 cgggatccgc caccatacca tccaccagag gcggcaccca actccaatcc gcttcggcaa
     2881 atttcggtga ctcgtggcga tgcaccacat gcacctccgc cgcgccgcca tcattcaaca
     2941 acgccgccca ttcaaaagcg ctttgcctgc cacccaagat taacacccgc ctgcctttat
     3001 attcagaaag ttgcacggca tcacaggtgt gttcatatct accaacaggc aaaatgtctg
     3061 ttaacgcggc gggataattc ttgaaatagc ccatgccaat ggcaatggca acgtttttgg
     3121 cttcaatgac ctttccatct tcaaaggtcg caaggaaacc atcttccttt tggtctaagc
     3181 gtgtgacata ttcagagatg gtctcgattc ctgctccaga ttggaaccac tgagcgtact
     3241 gtaaatagaa ctcaagagag agcggctcaa catctttcgg agtcttacct aatgtttcca
     3301 gataattcat aatggaaaaa cgatcggttg ggtccagcga ccagtctgac gcagagcgta
     3361 ggaacatgcc cgcgggcatg ttcttcttcc aaaattccat aggtttgcct gcaacgagaa
     3421 agtccatgcc taaagatttg gcatatgccg ccaaagacaa accaaaaggg cccgcaccaa
     3481 tgataagtaa atctgtttgc attctactct ccttaattaa cgaaaattcc aagccaaaac
     3541 tgatatagcc caaagccgaa caagacaatg caggaaagcc caagtaaaac acgatttaac
     3601 ctgggcccca aacgagatgc ggctccgaag acaatgatca ttgcgatcag gcaaataata
     3661 aaggtcgtat aaaacccgat caagagaccc aagccgttga taggcgtttc gcgccagcct
     3721 tgcaccaaaa taggaccagt cacgaacatc caaaaaagat aagctccggg gctgagggca
     3781 ttcgtcatcg ccgctttgaa aatggtctgc tgacccgata attccggatg cgcaagtgtc
     3841 tcgtcaaaat tacgccagga tttatatgaa ccataagcaa gatagagaac gaatagcccg
     3901 cccacaatat acatggcacg ctgcgcccaa tcgggtagtt ggcttaatac cagcacactc
     3961 aacaaaatga taggaccatc actgaggaat ggcgcaaatg cgccgggcag ggcacgcttc
     4021 cagccgcggg ttaaggcttg agaaatcaga aacgtttgaa agggtccgat ctgagaggcg
     4081 gcggcgaagc cgtatccgac accctgaatg agataaaccc acatatagat ttattgccca
     4141 agtaagtatt ggtcaaagaa agcggataca cgcgcttcga attcttcagc gcgttcgctg
     4201 tcaaaatcca catgcccgag ttcggggtcg aaccaaagtt cttttggctc tccggcggca
     4261 tcataaagaa tttgtccgct tgtgggcgaa atgaccgtgt ctttgccgcc ttgcattaag
     4321 aacaccgggc gcggactgat attgggaatc cactttgttg catcaatatc gtcgatgctg
     4381 ataccggtca tgttctctgc ccaaaagaca ataagtggca caaatggaaa atctggcaag
     4441 cctgtgaaat gacgcacact ggtggaaact gtatctttca tagaagagaa agcacaatct
     4501 gccacaacgg ctttgatgtt ttgattctgc gcagcatatt gaatggaaag catgccgcca
     4561 tacgaattgc cgagaatacc gatcttttcg ggatcaatgt catctcgcga aataagatat
     4621 tgataccacg cttcgaggtc tgccatttca taatatccaa gtgtgatgag ttctccttca
     4681 ctctcatcat gagcacggac cgtggtaagc aaaatgccgt agccatgccg gtgcatcatt
     4741 tcagcttcgt tgagcatctc actgcgcgtg gatttatacc cgtgctgcat gatgataacc
     4801 gccccatttt cagatgggat aaaccaaccc actagcttca taccatctgg gttggtaaca
     4861 gtgacgtctt catattgcat gttgtaatca gaaggcagtt cattgggcag ttcacgatct
     4921 gccatgggag catgcaataa gttatatgct tcctgtcttg ttttccataa gatccctgct
     4981 gcccctaata caagaattcc aacgaccgcc cctaatacaa acttgatact acgcttcata
     5041 aattcatcct ttggcaatag agatgcaagc aatgggtcaa ttttgccaca gagttaaaag
     5101 gcgcagagag tgaatttctc tgcgcctttt gttgtatagc tcaattattt gtttaaagct
     5161 tcttttacag cttcgtacac gtccaccacg ccaaagccaa aggcgttgct gggaatatcg
     5221 ccgctaaaac agcccagcga acgatccccc atataaggct tagcagtatc gataattagc
     5281 tgttctgtcg catcaatatc accgatcaac tcaggctggg cagaccagat caacgctacc
     5341 acccccacca tatgcggacc tgccatcgat gtaccgctat tggagccgta cgaattttcg
     5401 ggtaaggatg aaaacacatc cacacctggt gcaacgatat cgggtttgat gcgcccagag
     5461 ccgtccgcag tgacaggtcc acggctgctg aacctagcga ggtctccaga ttggtcaatt
     5521 gcacccacag agaagactga gtcataaaga gataaaggag cctctaccgt ttcgcagttc
     5581 ggtccgtcat tgccggtaga aaccaccaca aaaatgccgg cgtcacgcaa atggtttgcc
     5641 gcataaagca aagcattcgg gtcacagcct tcaatcgaag ggcagcccca tgaattgttc
     5701 atgacatgtg cagcttgagt tgggtcacca tctacaaagg gatcaccgcc aatcgggaag
     5761 ggcgcgagca tgaactgcat acaatctaaa tacaaagctg ggttagcaag attacggtca
     5821 aggttcacgc acccgatcca ttgtgcatca ggagcaacac cgatgccatt atttccaagg
     5881 atcgttccca tagtgtgagt gccgtgcccg ccttcatcat tcggtgatgt tgtgccatcc
     5941 cacgggtcga accagttgta atcatcactg ccgttataac cacgatactg tttttcgatc
     6001 gcaggatgat tcccatctac acccgagtca gattgcccta ctacaatgcc ttcgccgcgc
     6061 acgccgaact cttcccagac tcgatctgcg ccaataagtt gaatgttcca accaacataa
     6121 tctgcgggcg gcgcagactc aaagcccggt tcaggagaat cttcgggagc agcacgcagg
     6181 cgcgggctag gaatgacgcg gtccacttcg gagcgggtca tcaagaacag gcgcacaaga
     6241 gtcccaccct gcacttccat cgcgttctcc aaataatatg gcgtatatgc cacaccaaat
     6301 gtatctagcg tttcacgcag gtctgcttgc gttttatttg catgatccgt aaggcgcgta
     6361 taagcttcgg tcaaacgctc atcgcggttt ttgatggatg ctacatccga aatatcggct
     6421 tggtctttga agataacaaa gaggcggtca ctataattgc cggggtttcc ccagatgaaa
     6481 aaagcggcga tgaccatgac ccaccctgcc acagcaccta cccatcccgc cgcccgcttc
     6541 acacctgacg ttgagcctga tcccattacc tgacgcagaa tcaacgccac aacactgact
     6601 ccccaaccga tcaacaaagc aatccccaca gctttcacgg caagcccgcc aatatcaccg
     6661 agcacaatgg tgagttcagt tgggtcgaag aatgccaagc cagcaaatgc aagcaaccca
     6721 gccgcaactg tagctgccgc ccgcgatgga tataaggctg cgatagcgca accaaaagca
     6781 ggaactaaaa caaccaatat caattggctt ccgtcatacc caaacgaaga agccagtaat
     6841 gccaacaaac cgcctgcgcc aatgccatta agaaagatat tttcagcatc tgacattaac
     6901 acggcggcaa gcaagccaaa tgccaaaccc gcaaacagac taagcaccac atcggcgggc
     6961 gacccgaacg agccatacac tgccagcgga gcaaacccca atgccgcaac caagaacccg
     7021 aatggcaacg cactgaaatc ccaaactagt ttgtccttac gaatatagat caccgccaag
     7081 gcaacgataa cggcaaggac aaactgcaaa atagaaccaa gctggtcgtt gtttggtccg
     7141 agtgcacgca aaagcaaggc tggaattcca aacaaggaag cggcaaacaa gcttgcatac
     7201 catcccttga agcgatcgtc actggtaagg taccaaagca agccaaacac caggctcatc
     7261 aggaagattt gcacacccaa accaataaac cctgcccatg ccaattgctt catactcgaa
     7321 gaggcaattg cgctctgttc ggtagaccaa acaatgaagt tgtagataaa caaacaaaat
     7381 ggcattggca aaagcacaat gaggaataat gccaaggcgc cattgctgtg cttcttttct
     7441 acaactggcg tattttgagt aaattcattc atggttcact ccttgatcgt ttcacattta
     7501 ttctgcgccg ccttcaatac cagcttgcac ttcttctcgc tcggtatgag cctgcacctt
     7561 gaacactttg aagccccatt gaacggaggg tccgatcaaa acagcatgga caaccgtgcc
     7621 aattccggcg ggtccgccta acagccagcc aagtaccacc accgtcactt caatgatcgc
     7681 tcgcgatacg cggatgctca cgcctgtctt gcgccgaatc gcaagcatca agctgtctcg
     7741 tggaccagcg cctgcgtcaa ctccaatata gatggcgctg gcaagtccca taatgatgat
     7801 ggcgcttaac aacatgagta tctgtatagc aagattgtta ttcacagtag gaaccaacgc
     7861 catgcataag tccacccagg gaccaatgga aagaatattt cctaacgttc cccaccctat
     7921 ttgttcccgc attaacaagg agagtatcaa aacgatgaag cccataataa cagtcatggt
     7981 acccaccgta atgtgtaaaa agtctgccaa tgccacttcc aatactgccc aagcacttgt
     8041 tccgaggttt ccgcgaatca ttaaggtgat cgccaagcca tacaaaagaa agccgatctg
     8101 aatgcgaata aaatcgcgga taaatgtatt ccaacgaact ggtttaatca tgatgctcct
     8161 ttgaaagcat catgatacca tgccgctata aaccttgatt ttttgtgatg cgattattta
     8221 tctgagtaaa ttacgacttt attcctaccc tgttgttttg cctgatacaa agcctggtct
     8281 gctcgacgga tcatatcttc gatcgactga tcttgaggtt caaaagataa ttctgcgatt
     8341 cccatgctga tagtaacaaa cgtggagaaa ccacccatgt caatatagac atctgcaact
     8401 gctttacgaa tacgttctgc aatgagaaaa gcctgctcta catttgtctc tggcaaaaga
     8461 atcgtaaatt catcaccacc gtagcgtgcc aaaatatcca cttcacgtac ttgtaaattt
     8521 gctgttttgg aaacctcaac caatgtgcga tccccaaaat catgtccgaa tgtatcgttt
     8581 gcctgtttga aaccgtctac gtcaaaaata atcaatgtca atggtctttg atagcgaatt
     8641 gcggagttaa actcgcgtgc ggcaagctca aagaaatagc ggcggttgta taaacgcgtc
     8701 aaaccgtcgg ttcgagcaag aatctgttca tactcgaaca attgctgaag ttccttatgg
     8761 gcaatttgaa gagaactatt ggcaaggcgc aggtcatctt ctgctttctt tcgctcggta
     8821 atattacgag tcacaccaat cgcttctatt gtagcgtttt cattcataac aaatgtgctt
     8881 gaaacttcaa ccacaataat tgaaccatct ttatgaacat ggtccaactg ataaagttct
     8941 gttacagatg ttttatctcc agcgaggaag cgctttatgc gttcaggggt gatgctctca
     9001 atagccatca tagcagaagg agttaacacc atgctgacat gtttattcag caattcctca
     9061 gcagaatgcc ctaaaaggtt ttggacagac gagctaatat atttgaattc aagagttgcc
     9121 agatccaaaa cccaaaccac atcggcaatt cgttcagcca ataggcggta ttgtttctcg
     9181 ctttccttaa gtcgtttttc tgctgctttt cgatccgtca catcgatcag actgccttcg
     9241 taataaagcg gcgtaccttt cccatcatac acagcacgga cataatcttc tgcccagaat
     9301 ctggtcccat cttgacggat aaattcagac tcatatcgat tcaagaaacc attttcagaa
     9361 atctgctgtc ggaactcgtc atctaaagca gggtcattat aaaactccca tgccttcttc
     9421 cccttcattg tttctaaatc ttcataccca aacattcgca acaaagatgg gttggcatcc
     9481 agcaactcgc catggataga agaacgatat agtccgattg gcatgttctc aaacaaagaa
     9541 cgataggctt gttcagattg gatcaatgct gattggatct tcttacgttc agaaacatct
     9601 cttgccaccc agatggttgt atcgggtccc aacggcgaga ctctcgactc gaaccatggc
     9661 gaaacttctg ccaaaatgat ctgatattcg ataatgaccg tcttaccggt tgctagcaca
     9721 tattcaattg ccttgagaaa ttcctgaact tgctcttcag ggaagaacgc ggatagtccc
     9781 ttcccgatca cctcggcagg agggatataa agtgtttccg ggtttgtagg agctacactt
     9841 tgatagattc catttcgatc aatcacgata acagaatctt tcattgaaga gaataatgcc
     9901 cgtagctcag cttcagactg aacaagggct tcttcagctt tgacttcctc tgtaacattg
     9961 ttacaaacga tcagaatcac caaatgtcca tctgtattcc tgatagcacg cgcaaactct
    10021 ctaacccata gaatgctttt atctttcctt tgttttcgcg cctcaacctg aacggtcttt
    10081 tcaggatcgt tgatacacgc caacatcttt tgcagaacat gttcgcggtc atctggataa
    10141 aagatgtcca acaatgggct gccgatcagt tcatcaatgg cataccctaa ttgttcc
//
LOCUS       DAOQFT010000007        10005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3138_length_10005_cov_8.562111, whole genome shotgun sequence.
ACCESSION   DAOQFT010000007 DAOQFT010000000
VERSION     DAOQFT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3138_length_10005_cov_8.562111"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1945
                     /locus_tag="PLR93_00320"
     CDS             83..1945
                     /locus_tag="PLR93_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH25427.1"
                     /translation="MKPLKISLSVKLVFAFLFLSLIPLGIVIYFNAVFGSEHLQEDAD
                     RQLGSTAKLAANNINVFLTGGLDHIQSTAALAEFEEYLSLPASERKGSEIEQEVYQNL
                     QATVLLNPAYITSVGVMDLNGVNLVDTNPTQIGERENEKEFFIQTIETKKPYASNAEL
                     DEGEYSIYFSAPIFDDNNKTLGIVRIRYDANVLQDILLKTAEDSSIEGVVIDLFDENH
                     VALAVTDAPEDILKILVSPSDEKLKELQEAGRILEGSAEEVALNDPDLETHLRNAEQT
                     PFFTIESEGEEAATAFIQSKEWIVLAGQAQSIYQAPLQEQLRIEITIGLALLVIVSVL
                     ALLMARSISRPIIDLSGVAKDIANGNIDLQAKVQTTDEIGELATSFNDMTSRLRSSFI
                     DLERRAKEITTISEVSRRLSSVLDKQRLLIEVVEQIKSSLDYYHVHIYLLDKDSGDLI
                     MSGGTGEVGQTMLERKHRISKGRGLVGRAAESGAPVLVPDTLKDASWLPNPLLPETAS
                     EIAVPILSETGVVGVLDVQNNIQNSLSERDVELLQSIAYQVSIALKNADTYMEAQSQA
                     ERENIINEINRKIQNTSSVEQALQVAIRELGSTLGAKDSRIVLHLPDSVTKESR"
     gene            1959..7352
                     /locus_tag="PLR93_00325"
     CDS             1959..7352
                     /locus_tag="PLR93_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH25428.1"
                     /translation="MNTPNPSDDAMKKRIDEMFSGQELPSYASLGEVNAMKARIRELE
                     EKLYQSTKPAVASLKLEEVNSVAEEKVTEKRPKSGILSLLTPPSFGNLAKDRIAKLQH
                     QILLGIFTTAILALIAVFLSWNGSGSLFGVGILSFEFVVLAFAFYFLRRGNLQVVSWI
                     LIGLIYSILIASLFVSGFTPATVIELALIVTMAGLLLHPWQVVIVSISAIVMNFVGPN
                     IGVPTTLPDNNLIFTGIILALEGALLTIASSTLENTFKEIDRSTKALVISNKNLQDIS
                     ANLEERVASRTYDLELATEVGKILSGKVDNLFGLLEDAVETIRARFNLYYTQVYITDP
                     SGRTITLRAGTGDVGKQLLKRGHHLLINPSSLNGRAASEKRTVIVSNTTDNPNFLPNP
                     LLPFTRSEMSVPLIAGDRVVGVLDMQSDQANFLTANNADAFEALAGQLAIAIQNAALF
                     EQVKLSRLELEAQTKKQSYENWQNFLNAIEREEKLGYVFDQSDVLPLIKAQGIEQTST
                     ALTAPIQVAGADIGNIQFIDNAERVWTPAEEDIVQNVVSQLSVHMDTLRLLAESDKYR
                     QDAELSTKRLTVEGWNEYLKSRQNTTEGFIYNDHKVLPLNGADIKTIGKEAVIPLQVQ
                     NQSIGEIAIETTEEFSDEELDILSNVSRQLSNHIENLRLFEQSNDSRKALEVTLQQLS
                     TNEARLSEALDIARLGNWEYDVEKDIFTFNDHFYAVFRTNLQEVGSYQMSSAEYAERF
                     VFPEDAPLVGIEIGKAMTTTERHFKQHLEHRIIFPDGSLGYISVNINVERDENGKILR
                     WYGANQDITDRKMAERAVYEREEQFRNTLNNIPTAIVVTRMSDGNVLYANEALASMFG
                     APTQELIGKRSPDFYYNTNDRQRVVDIVRKDGYVKNNELLFKRITGEQFWALFNVYPL
                     KFFGENALMGSFYDLTERKEAEEAVRKAQEQYTLAVDGSNDGLWDWNIETNEVYFSPR
                     WKSMVGYEENELMNGFADFEALLHPDDHDRVLGVVNDYLTGKIDDYNVEFRFRHKNGS
                     YRWILARGKAMRNSDGAPYRMAGSHTEITERKEAEELIRTNEARLAEALEIARLGNWE
                     YDVENDIFTFNDRFYSVFRTNIQEVGSYQLSSAEYAQRFVHPEDLPIVGVEIEKALTS
                     KDRFYAASLEHRIVFPDATTGYISVNINVERDENGKITRYYGANQDITERKQSEELIA
                     KRAEQLSVLNRVMEVANTSQDKYYILQTATDEFRSLMQGFSAGVLLLDERGDMLTLVT
                     ESYADPSMPKLAGRSMPISVNPATNSSIKSGKTVLVADAQNDPILEPIRAIMKQRNIQ
                     SVLVTPMFSRNKVIGVFSIDTNDPNRSFDNDDITLIETLAKQLASAIENVQLFDEIQH
                     RAADLSTVATVSTTTATVLDPDELLKTVVNISKERFGLYHAHIFLNNDDELVLTSGAG
                     EVGQKMLESGLTIDINAEKSLVARAARGRQAVIINDVTKEEGFLPNPLLPNTQSEMAV
                     PMLVGESVLGVFDIQSDKINAFTEEAADIYTTLASQVAVALQNARLYQEQASVVTQLR
                     ELDRLKSSFLANMSHELRTPLNSILGFADVMLEGLDGPLTSTMDNDLRLIQKNGQHLL
                     HLINDVLDMAKIESGRMSLHADTFNVNNLLEEVTSITSSLASDKNIALYIEPESDREV
                     EIYADSTRIRQVMINLVNNSIKFTEKGSVALNVSRMDDARVLITVKDNGLGIPTDQLE
                     TIFLEFTQVDTTTTRKVGGTGLGLPISRRLVEMHGGRLWAESSGIDGEGSTFFVELPL
                     ETRVTEIIEKREK"
     gene            7353..>7667
                     /locus_tag="PLR93_00330"
     CDS             7353..>7667
                     /locus_tag="PLR93_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25429.1"
                     /translation="MAMPLYLECNLCNHQQAYIPFVPAICEKCESQWVEARYDYESFK
                     REILRGIPNRPNNLWRFQDVLPLKNPSALDLYPAGGTPLWLSQRFAPDLGHGSVYIKD
                     ERY"
     assembly_gap    7668..7767
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7768..9163
                     /locus_tag="PLR93_00335"
     CDS             <7768..9163
                     /locus_tag="PLR93_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNH25430.1"
                     /translation="RFAPDLGHGSVYIKDERYSPTSSFKDRQAAVAVAAMNENGIREA
                     VIASTGNAAVAYAAACARAGIKLWVFMTSRVPQEKLREAALFGAEVIRVSGNYDQTKQ
                     IASQFAQRKNLLLDRGASSVPARESMKTIAYEIVEQLGWRAPDWYIQAVSGGLGPLGV
                     YQGFKEMFNMGLIKKVPKLAIIQSAGCAPMVNAFKEGKDVATPVIPDTRITILSTGDP
                     GKAYTYLWNLTQQYGGVMESVTDLEAYEAMKTLAKSEGMAVEPATSVAFAGFQKLSGQ
                     GIISKDELVAINCTGHTFPVEKHVLGDQWAVDVHLSKDQSPAPHEGLQAALENLDEKT
                     TTILLVDDNSDDALLIRRLLEGRKNYRMYHAKDGWEGLSVARQKLPDLIVSDLTMPGI
                     DGFGLVEELKLDPRTKDIPVVVVSAKDITKEERLRLNGQIEALYQKGSLPTRKFVDKV
                     IGVIEDKFEEKGKE"
     gene            9173..9556
                     /locus_tag="PLR93_00340"
     CDS             9173..9556
                     /locus_tag="PLR93_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25431.1"
                     /translation="MGNIILCIEDNPDNMMLVKRALESRGYVLLEAFTGNKGVEVAEQ
                     NNVDLILLDINLPDIDGYEVARRLRSSSKHNLAYTPIIAVTANALKGDAEKALAAGCD
                     VYMSKPINIRELWARVEAFVPAPDP"
     gene            complement(9617..>10005)
                     /locus_tag="PLR93_00345"
     CDS             complement(9617..>10005)
                     /locus_tag="PLR93_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25432.1"
                     /translation="SEMLDLRNRELAEAAKVDDFIVSEHLISLMMAQLSENAELKDVF
                     TDLFDPEGSEVYLKPISEYVTTGEAVTYSTVVEAAKRRGETAIGYRIMSEVHDAEKSY
                     GVRTNPKKSEKVTFASEDKLIVISEN"
BASE COUNT         2863 a         2322 c         2257 g         2463 t
ORIGIN      
        1 agtagcagtc cgtgaggtcg ggcgcgcttt gaatacccag gcaaaagtga aaatcaatac
       61 cacaggtaaa ggataaaaga aaatgaaacc attaaaaata tccctatctg taaaattagt
      121 gtttgctttc ttgttcttgt cactcatccc attgggtatc gttatctact tcaatgcagt
      181 ttttggtagc gaacatttac aagaagatgc cgatagacaa ttaggaagca ccgcaaaact
      241 agctgcgaac aatatcaatg tatttctcac tggcggctta gatcatattc aatctacagc
      301 ggctcttgcc gaatttgaag aatacttatc attaccggct tcagagcgaa agggaagtga
      361 gatagaacaa gaggtttatc aaaaccttca agctaccgtg ttgttaaatc cagcttatat
      421 cacttcggtc ggagtcatgg atctaaatgg cgtcaacttg gttgatacaa acccaacgca
      481 aattggcgaa cgagaaaacg aaaaagaatt ctttattcag accatagaga caaagaagcc
      541 ttacgcctct aatgccgagc ttgatgaagg cgagtattcg atctatttta gcgccccgat
      601 tttcgatgac aataacaaaa cccttggcat tgttcgaatt cgctatgatg cgaatgtctt
      661 acaagatatc ttacttaaaa cagctgaaga ctcaagtata gaaggggttg tcattgactt
      721 attcgatgaa aatcatgtgg cattggctgt aacggatgct ccagaagata ttcttaagat
      781 actggtctca ccttctgatg aaaaactgaa agaactgcag gaagctgggc gtatattgga
      841 aggttctgca gaagaagtgg cattaaatga cccagatcta gagacgcact taagaaacgc
      901 agaacaaact ccctttttta cgattgaatc agagggagaa gaagctgcaa ctgcttttat
      961 tcaaagcaaa gaatggattg ttctagccgg gcaagctcaa tctatttatc aggcaccact
     1021 tcaagaacag ctacgcattg agatcaccat tggtcttgca ttgctagtca tcgtttcagt
     1081 ccttgcacta ttgatggcaa gaagcattag ccgccccatt attgaccttt ctggggtagc
     1141 aaaagatatt gcaaatggga atatagattt acaagcaaaa gtccaaacaa ccgatgaaat
     1201 tggagagctg gcaaccagct tcaatgatat gacctcaaga ttacgatcga gtttcattga
     1261 tttggaacgc cgcgccaagg aaattaccac catttccgaa gtgagccgcc ggttatcttc
     1321 cgtgttggat aagcaacgcc tcttaataga ggtcgtagaa cagatcaaat cctccctgga
     1381 ttactaccat gtccacattt accttttgga taaggacagc ggcgacttga tcatgtctgg
     1441 cggaaccggg gaagttgggc aaactatgtt ggaacgaaaa cataggatca gcaaagggcg
     1501 cggcttggtc gggcgtgccg cagaatcagg cgcccctgtt ctggtgccag acacgctaaa
     1561 agatgcaagc tggttaccta acccgctttt gccagaaacc gcttctgaaa ttgcggttcc
     1621 aatcctttca gaaactggag tggttggggt tttggatgta caaaataata tccagaacag
     1681 tctatccgaa agagatgtgg aattgttgca gtccattgct taccaagtat cgatcgcctt
     1741 gaaaaatgca gatacgtata tggaagccca aagccaggca gagcgcgaaa acatcatcaa
     1801 cgaaatcaac cgaaagattc aaaatacttc ttcggtagag caagccttgc aagtagccat
     1861 tcgcgaacta ggttccacat tgggagcaaa agattcgcgg atagtgttgc acctgcctga
     1921 ttctgttacg aaagaaagcc gttagaagga gaacctttat gaatactcca aatccatctg
     1981 atgacgccat gaaaaaacgt attgacgaaa tgttctctgg gcaagagtta ccttcctatg
     2041 ccagcctagg ggaagtaaat gccatgaaag ctcgcattcg tgaattggaa gaaaaactgt
     2101 accaatccac aaagcctgct gtagcttcac tgaagttaga agaagttaat tctgtggcag
     2161 aagaaaaagt gaccgaaaaa cgtccaaagt ctggcatctt aagcctacta accccgccaa
     2221 gttttggtaa tctggcaaaa gaccgtattg ccaaattaca acaccaaatt ttgctgggta
     2281 tctttacaac agcaatcttg gcgctcatcg ctgtttttct aagctggaac ggctcaggtt
     2341 ctttgttcgg ggtgggcatt ctgtccttcg aatttgttgt tctagctttt gccttttatt
     2401 tcttacgccg cggcaacctt caagtggtga gttggatatt aattggcctt atctattcca
     2461 tcttaattgc cagtctcttt gttagtgggt ttacgccagc aaccgtaatc gagctggcgc
     2521 tgatcgttac catggcaggt ttgcttcttc atccgtggca agtggttatt gtgtctatca
     2581 gtgctattgt gatgaacttt gtaggtccga atatcggtgt cccaaccaca ttaccagata
     2641 ataatcttat cttcaccggc atcattctag cgttggaagg tgctttatta acaattgcat
     2701 ctagcaccct ggaaaacaca ttcaaagaaa ttgacagaag tacaaaagcg ttagttatca
     2761 gtaataaaaa cttacaagat atttctgcca accttgaaga acgcgtagca agccgtactt
     2821 atgaccttga attagctacg gaagtcggta agatcctatc tggcaaggta gataacttat
     2881 tcggcttatt ggaagatgcg gtggaaacaa tccgcgctcg tttcaatttg tactataccc
     2941 aagtttatat tacagatcct tctgggcgca ccatcaccct ccgtgcagga actggagatg
     3001 taggcaagca attgcttaag cgtggtcacc accttttgat caaccctagc tcattgaatg
     3061 gacgtgccgc ttctgaaaaa cggaccgtta tcgtttcgaa cacaaccgac aatccaaact
     3121 tcctccccaa ccccctactc cccttcaccc ggtctgaaat gtccgttccc ttgattgcag
     3181 gtgaccgcgt tgtaggcgtt ttggatatgc aaagcgacca agccaatttc cttaccgcaa
     3241 acaatgctga tgcgtttgaa gctttggcag ggcagttggc aattgccatt caaaatgcag
     3301 ctctctttga acaggtgaaa ctatctcgtt tggaactcga agcccaaaca aagaaacaat
     3361 cctatgaaaa ctggcaaaac ttcctgaacg cgatcgaacg ggaagaaaag cttgggtatg
     3421 tctttgacca aagtgatgtc ttaccgttga taaaagcaca aggtatagaa caaacctcaa
     3481 cggctctaac tgcccctatt caagtggctg gtgcagacat tggcaatatc caattcatag
     3541 acaatgctga acgggtctgg actcctgccg aagaggatat tgtacaaaat gtagtttccc
     3601 aactttcggt tcacatggat accctcaggc tattagcaga gtctgataaa tatcgacaag
     3661 atgcagagtt aagtacgaaa cgtctaaccg tagaaggctg gaatgaatac ctaaagtcac
     3721 gtcaaaacac gacggaaggt ttcatttata acgatcacaa agtacttcct ctcaacggcg
     3781 cagacatcaa aaccatcggc aaagaggcgg ttattccgct ccaggtgcaa aatcaaagca
     3841 ttggcgaaat tgcaattgaa acgaccgaag agtttagcga tgaagaactg gatatccttt
     3901 ccaatgtttc acgccagttg agcaatcata ttgaaaactt aagattgttc gaacaaagca
     3961 atgacagtcg aaaagcgttg gaagttacac tacaacaatt aagcaccaac gaagcccgtc
     4021 tttctgaagc gctcgatatc gcccgccttg gtaactggga atatgacgtt gaaaaagata
     4081 tctttacatt caacgaccat ttctatgctg tattccgcac aaacttacaa gaagtcggaa
     4141 gttatcagat gtcttctgct gaatacgccg agcggtttgt attccctgaa gatgctccat
     4201 tggttggtat cgaaatcgga aaagcgatga ccaccacaga gcgccacttc aaacagcatc
     4261 tggaacatcg cattattttc ccagatggca gccttgggta tatctcggtc aatatcaacg
     4321 ttgaacgcga cgaaaatgga aagattcttc gctggtatgg tgccaaccag gatatcaccg
     4381 accgcaaaat ggcagaacga gcagtctatg aacgtgaaga acaattcaga aatacgctca
     4441 ataatattcc aactgcaatt gtggttaccc gcatgtctga tgggaatgta ctatatgcaa
     4501 atgaagcatt agcaagcatg tttggcgcac ctactcaaga actaatcggt aaacgatcgc
     4561 cagactttta ttacaataca aatgaccgtc aacgagttgt ggacattgtg cgaaaagacg
     4621 gttatgtaaa gaacaatgaa ctcctattca agcgtatcac gggagaacag ttctgggcgc
     4681 tctttaacgt atacccattg aaattctttg gtgaaaatgc gcttatgggt tcgttctacg
     4741 accttactga gcgtaaagaa gcagaagaag ctgtccgcaa agcgcaggaa cagtacaccc
     4801 ttgcggtaga tggttctaac gatggcttat gggactggaa tattgagaca aacgaggtgt
     4861 acttctcacc acgctggaag tccatggtcg ggtatgaaga aaatgaatta atgaatggct
     4921 ttgctgattt cgaagccctt ctgcaccccg atgatcatga ccgtgtgctt ggtgttgtaa
     4981 acgactacct gactggaaaa attgatgact acaacgtcga attccgcttc cgccataaaa
     5041 acgggtcgta tcgttggatc ctcgctagag gtaaagccat gcgtaacagc gatggagctc
     5101 cctatcgtat ggcagggtct catacagaga ttaccgaacg caaagaggca gaagagctca
     5161 tccgtaccaa cgaagcccgc ctggctgaag cgcttgaaat tgcccgcctt ggtaactggg
     5221 aatacgatgt tgaaaacgat atctttactt tcaacgaccg cttctattca gtcttccgca
     5281 caaatataca agaagttgga agctaccaac tgtcatcagc agaatatgcc caaagatttg
     5341 ttcatcctga ggatctaccc attgttggtg tagaaatcga aaaggctctt acctccaaag
     5401 accgttttta tgctgcctca cttgaacatc gcattgtttt cccagatgct acaactggct
     5461 atatttctgt caacattaac gtagaacgcg atgagaacgg aaaaatcact cgttactatg
     5521 gagccaatca agatatcacc gagcgtaaac aatctgaaga gctcattgca aaacgtgcgg
     5581 aacaactatc tgtgcttaac cgtgtgatgg aagtggcgaa cacctcacaa gataagtatt
     5641 acatcttgca gaccgctaca gatgaattcc gcagtttgat gcaaggtttc agtgctggcg
     5701 tattgttgct agatgaaaga ggcgacatgt tgaccctggt cactgaaagc tatgcggatc
     5761 cgtccatgcc aaaattagca gggcgctcca tgccgatctc ggttaacccc gccaccaact
     5821 cctccatcaa atctggcaaa acggttctgg ttgcagatgc tcaaaatgac ccgatccttg
     5881 aaccgatccg cgccattatg aagcagagaa atatccaaag cgtgctcgtc accccgatgt
     5941 tctcacgcaa caaagtgatc ggcgtattct caatcgacac caatgacccg aaccgctcct
     6001 tcgacaatga cgacattacc ttgattgaaa cgcttgccaa gcaactcgcc agcgcgatcg
     6061 aaaacgtcca attgttcgat gaaattcagc atcgtgcagc agacctctct accgtggcaa
     6121 cggtcagtac taccacggct accgtgttag acccagatga actcttgaaa accgttgtga
     6181 atatttcgaa agaacgcttc ggtctgtatc atgcccatat tttcttgaac aacgatgacg
     6241 aattggtgct cacttcaggg gcaggcgaag tcggtcagaa aatgctagag agcggtctga
     6301 cgattgacat aaacgccgag aaatccctgg ttgcacgtgc cgcccgcgga agacaagccg
     6361 tcatcatcaa cgatgttacg aaggaagaag gcttcctccc caacccatta ctgccgaata
     6421 ctcaatctga aatggctgtg ccgatgttgg taggtgaaag tgttctgggt gtgttcgaca
     6481 ttcaatcaga caaaatcaac gccttcacag aagaagccgc cgatatttac accacacttg
     6541 cctcacaggt cgctgtagca cttcaaaatg cccgcttgta tcaggaacaa gcttcagttg
     6601 taacccagct tcgcgaatta gaccgcctca aatcctcctt cctcgcgaac atgtctcatg
     6661 agttgcgaac tccgctcaac tccatccttg gcttcgccga tgtgatgctc gaagggttag
     6721 acggtccgct gacctctaca atggataacg acctgcgctt gatccaaaag aatggtcaac
     6781 atctgttgca cctcatcaac gatgtgctcg acatggcgaa gatcgagtct ggtcgtatga
     6841 gcttgcatgc cgatacgttc aatgtcaaca acctgctcga agaagttacc agcatcacat
     6901 catctctggc aagcgataag aacatcgcct tgtatattga accagaatca gaccgtgagg
     6961 tcgagatcta tgctgatagc actcgtatcc gtcaggtaat gatcaacctt gtgaacaact
     7021 ccatcaagtt caccgagaag ggaagcgtcg ctctgaatgt ctcgcgaatg gatgatgccc
     7081 gagttctgat taccgtcaaa gataatgggc ttggcattcc aaccgaccag cttgaaacca
     7141 tcttcttgga attcacacag gtcgatacca ccaccacccg caaggtcggt ggaacagggt
     7201 tgggtctgcc aatcagccgc cgccttgtcg aaatgcatgg tggtcgtctc tgggcagaaa
     7261 gttccggcat tgatggtgaa ggctctacat tctttgtaga attgcccctt gaaactcgcg
     7321 ttacagaaat cattgagaaa agagaaaaat aaatggcaat gccgctttac ctcgaatgca
     7381 atctttgcaa ccatcaacaa gcctacatac cattcgtgcc tgccatttgt gaaaaatgcg
     7441 aatctcaatg ggtcgaagct cgttacgact acgagtcgtt caaacgtgag attctgcgcg
     7501 gtattcccaa ccgcccaaat aacttgtggc gctttcagga tgtcttgcca ttaaagaatc
     7561 catctgcact cgacctatac cccgcaggtg gaactccact ctggctctca cagcgcttcg
     7621 ctccggacct cgggcatggt tctgtttaca tcaaagatga acgctacnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg cttcgctccg gacctcgggc atggttctgt
     7801 ttacatcaaa gatgaacgct acagcccgac ctcttctttc aaagatcgtc aagccgcggt
     7861 ggcagtggcc gccatgaatg aaaatggcat tcgagaagcg gtcattgcct ccacagggaa
     7921 tgctgctgta gcctatgcag ccgcttgtgc acgtgcaggc atcaaactat gggttttcat
     7981 gaccagccgt gtgccacagg aaaaattgcg agaagctgcc ctctttggtg cagaagtgat
     8041 ccgcgttagc ggcaactacg accagaccaa gcagatcgcc agtcagttcg cgcaacgcaa
     8101 aaatctgttg ctcgaccgcg gcgcaagttc tgtgcctgcc cgcgaatcga tgaagaccat
     8161 tgcctatgag atcgtagaac agctcggctg gcgtgccccc gattggtata ttcaagccgt
     8221 tagcggcggg ttgggaccgt taggcgttta ccaaggtttc aaagaaatgt tcaatatggg
     8281 tctgatcaaa aaggtgccta aacttgccat catccagtct gcgggttgcg ctcccatggt
     8341 caacgccttc aaggaaggga aggatgttgc cacgccagtc attcctgata cacgcatcac
     8401 catcctatcc acaggcgacc ccggtaaagc atacacctac ttgtggaatc tcactcagca
     8461 atacggcggc gtgatggagt ccgttacaga ccttgaggca tacgaagcca tgaaaaccct
     8521 cgcaaaaagc gaagggatgg cagtggaacc cgcgacttct gtagcatttg cgggttttca
     8581 aaagttatct gggcagggta tcatcagcaa agatgagctt gttgctatca actgcacagg
     8641 gcataccttc cccgtagaaa aacacgtctt gggagatcaa tgggcagtgg atgtgcatct
     8701 tagcaaagac cagtcccccg ctccacatga agggttgcaa gccgcgctcg aaaacttgga
     8761 tgaaaagacc acaactatct tattagtgga cgataactca gatgatgcac tgcttatccg
     8821 ccgtttgctg gaagggcgga agaactaccg catgtatcac gccaaagatg gctgggaagg
     8881 tctctctgtt gcacgacaaa aattgccaga cctgatcgta agcgacctga ccatgccagg
     8941 tattgatggg tttggtttgg tagaagaatt aaagctagac ccacgtacaa aagatattcc
     9001 tgttgtggtc gttagcgcaa aagatataac aaaagaagaa cgccttcgcc tcaacggtca
     9061 aattgaggca ctatatcaaa aaggatccct ccccactcga aaattcgtgg ataaagttat
     9121 cggggtcatt gaagataaat ttgaagaaaa aggcaaagaa taaggagaac ttatgggaaa
     9181 catcatcctc tgcatagaag ataatccgga taatatgatg ttggttaaac gcgcactcga
     9241 atcacgcggg tatgttcttc ttgaggcttt cacaggaaac aaaggcgtgg aagtagcaga
     9301 gcaaaataat gtcgatctga ttttgttgga tattaactta ccagatattg atggctacga
     9361 agtagcaagg cgcttacgct ccagcagcaa acataacctt gcgtacactc ctatcattgc
     9421 cgtaacagcc aatgcgttga aaggggatgc tgaaaaagca ctcgccgcag gatgcgacgt
     9481 atacatgtcc aaaccgatca atattcgcga actctgggca agggttgaag cctttgttcc
     9541 ggcgcccgat ccgtaatttt tattcaacaa aaaacacccc gagcaaaaag ctcggggtgt
     9601 ttttattatc cgtctattag ttttcagaga ttacgatcaa tttatcttct gaagcaaagg
     9661 taaccttctc agatttcttc gggttggtgc gtacgccata agatttttca gcatcgtgga
     9721 cttcgctcat gatgcgatag ccaatagcag tttcaccacg gcgtttggcg gcttctacaa
     9781 ctgtagagta ggtaactgct tcacctgtag ttacatattc gctaataggc ttgagataca
     9841 cttctgagcc ttcagggtcg aagaggtcgg tgaagacatc tttcagttca gcattctcag
     9901 aaagctgagc catcatcaaa ctgatgagat gttcgctgac gatgaagtcg tccactttgg
     9961 cggcttcggc aagttcgcgg ttgcgcaggt cgagcatttc gctga
//
LOCUS       DAOQFT010000008         9778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3250_length_9778_cov_6.982104, whole genome shotgun sequence.
ACCESSION   DAOQFT010000008 DAOQFT010000000
VERSION     DAOQFT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3250_length_9778_cov_6.982104"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PLR93_00350"
     CDS             complement(<1..1304)
                     /locus_tag="PLR93_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HNH25433.1"
                     /translation="MKSLRWKQLALVALIIVGCTGPGSGSGVGSIFATDTPLPTPSVG
                     ITPAPNVDSAVTAFFDALKGDNYEEMYAMLSQSSRDALTLEDFSKRWTNALNEMSASS
                     FDVTVLSKSITPFNAEVKYSITYKTALVGDIQREMLMRLTNEDSNWKVQWDDALILPE
                     LAGGNLLKMEYSIPARGDIYDSKGLPIATQSDAFAFGIQTDQIDYDMIGALTTELGRL
                     CGLDPLDIQAQIDAAGPGWYLPMCEGTREEAQRLLSINPGGLVIQEYNSRYYFRGGLA
                     PQAVGYTLAISPEQLNQYKRLGYSGSERIGYTGVEQWAEDYLAGKHGGILRVVSPTGQ
                     VIATLGQSQPAPADSVYLTLDSNMQYYAQAAVEYFRGAIVVMEVDTGRIIAMASGPDF
                     DPNVFDSNNRNNQAVGNIFNDTRNPLLNRAAQGQLPLGSVFK"
     gene            complement(1323..1985)
                     /locus_tag="PLR93_00355"
     CDS             complement(1323..1985)
                     /locus_tag="PLR93_00355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNH25434.1"
                     /translation="MDKVKFVLASNSPRRRQLFGLGGWEFTVIVADVDETPFEHEPPR
                     DYVIRLAQAKASAVQPKAGLDAVILGSDTTVVDGNEILGKPVDESDAWRMLKQLRGRT
                     HQVYTAIAILQHGKMYTDLSMIDVPMREYSDQEIETYIKTGDPMDKAGAYAIQHPEFK
                     PVSSLEGCRAGVMGLPMCHVVRTLKHFNISSFADVPSACQSLLQYDCKVSPLILSGEI
                     VN"
     gene            2149..2718
                     /gene="xpt"
                     /locus_tag="PLR93_00360"
     CDS             2149..2718
                     /gene="xpt"
                     /locus_tag="PLR93_00360"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNH25435.1"
                     /translation="MNALQERILKEGKVLGGGILKVDSFVNHQVDPVLMDECGRAFAK
                     LFANVGATKILTAEISGIAPALTTGIHMGLPVVYARKTKPVTMPDQVYLTLAPSHTKG
                     RMVELIVSPEYLANNEKVLIIDDFLASGQTILGLVRLAEASGSKVVGIGALVEKSFEG
                     GRDALASVGVQIESIACIKSLDDGKIEFV"
     gene            2752..3099
                     /locus_tag="PLR93_00365"
     CDS             2752..3099
                     /locus_tag="PLR93_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNH25436.1"
                     /translation="MIRKILHLRFIVFLIGWIFAHMSFAIPVKRLRETSNLIAFQHPS
                     PSYKVHLLITPKRQVQSLAELDPQDTAFFTDLYSTVQSLVKELDLKAYRLIVNGGEYQ
                     DFPHLHFHLISEM"
     gene            3133..4668
                     /gene="guaA"
                     /locus_tag="PLR93_00370"
     CDS             3133..4668
                     /gene="guaA"
                     /locus_tag="PLR93_00370"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNH25437.1"
                     /translation="MNSIAILDFGSQYAQIIARRVRESQVYCELFPWDAPQEKILSIK
                     PKGFILSGGPKSVYEENAPFIQKFILESGLPILGICYGMQALTHALGGKVDPSAQREY
                     GHAEITPLVPGTMLDVLSKVWMSHGDKVTQLPEGFMALAKSGNSPFAAMGDMKRKYFG
                     VQFHPEVHHTPNGTELIKHFAIDICGVTPDWTPANIIEESVKRIREQVGDERVLAAVS
                     GGVDSSVAAALVHKAIGDQLVCVFVDTGLLRANEGAQVASAFREGLGAELITVDAADD
                     FLGALKGQTDPEMKRKIVGEKFIRTFEREAKNVGQPRFLVQGTIYPDVVESSGPDRSK
                     AAKIKSHHNVGGLPEDMQFELVEPLRYLFKDEVRLVGEALGLNSSLVWRQPFPGPGLT
                     VRCLGECTPERVARLRAADAILLEELSKAGFLGKGAQTSQAFVVLLPVRSVGVMGDMR
                     TYQEAAAIRAVATDDFMTADWARLPFELLAKVSSRIVNEVEGINRVVYDITSKPPATI
                     EWE"
     gene            4701..5603
                     /locus_tag="PLR93_00375"
     CDS             4701..5603
                     /locus_tag="PLR93_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25438.1"
                     /translation="MNFDYGNILTRALKLTWKHKSFWLLLMIPVFISFMILATIILPV
                     IFLTENEESMTLIMVLWAGIVIFGFFASFIIGAVSMNAVTLGILRAERGEGSTALMDV
                     VRDSFQYFKNAFGAVLIVQLSVGAVFTVFFLCMTALTLVTMGIASICLQPIMLLLTPL
                     SFLVGAVLYGALVAVIDEGLGALDAVKRAVQVVRDHVWKFVVISLIAYFGSMIVSSIF
                     TFPLMIPAMFGPALIESGVDITGNTFALIFIPFLCIFGVLMAFVSGVTGTFIAASMEI
                     SYLQLSRPVPEVIFAADAPKDATS"
     gene            5738..>6108
                     /locus_tag="PLR93_00380"
     CDS             5738..>6108
                     /locus_tag="PLR93_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25439.1"
                     /translation="MKIDLGKILTRSWQIIWNYKVLWIFGILAGFASGNGGSNGNSNS
                     SNRGSSYSGNSDQFVQQAKEFFQQYMLIIIAVCAGLVLLSLVFYALGMMGRIGILKGV
                     YKVEAGASSLMFAELWSESMP"
     assembly_gap    6109..6208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6209..6535
                     /locus_tag="PLR93_00385"
     CDS             <6209..6535
                     /locus_tag="PLR93_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25440.1"
                     /translation="IVKSDIGGMIVLSLVLGIGGGIIGLIIAIPVIIAIVPFVLGMAA
                     NNFQMGNSIPATAWVSLACCGLYFPVLLVLNGILTAYVKTSWALSYLQLSQPKENPPV
                     LAQDNA"
     gene            6528..8453
                     /locus_tag="PLR93_00390"
     CDS             6528..8453
                     /locus_tag="PLR93_00390"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNH25441.1"
                     /translation="MRKLFLTLLSISLLFGVVIVPAQAQGSITASVTIYNVDASNFPS
                     VNGFMDVTDANGIFASGLKPEAVTLVENGQPLPADSFNELAIPLQLVVAFNQGEALGA
                     QNANGISRYQRVEQIIEQWAQTRPADLPDDLSLVSQAGPVINHAPPADFVVGLKGFQP
                     DLRTATPNIQSLVSALDVVSAQTPRIGVKRAILFITPQMTDPNLAASVEPLIQRALEN
                     KIRVFIWYVDANTTFTTTSAAVFNNLAIQTGGTMFQFSGEERFPDPEEYFSPLRRIYN
                     FSYTSRVNTSGNHTVAVQVNLPSVGPLNSETLPFTLNIQPPNAFPVAPSIQITRQAPE
                     EDPFNTEVLLPETQEIEIIIEFPDGFDRPLARTTLIVDGIAVDENTAEPFTKFTWDLK
                     PYTISGEHQLTVEAVDSLGLSKTSMPLPVVVTVIKPPSGPAAFLAKYRMPLTFGAIIL
                     AGLVLFAILLSGRLRIRSLRVMQEERRVQNDPLTQSLEAFTEPAPVAVKEKTKKPRTK
                     STKAKAAKTLENEALASLVRLQADGQVAAVPPIQLNDAEIVFGTDPVQCSQILDDPSI
                     SSVHARIRATEDGGYLLMDAQSLAGTWVNYEPIPREGYRLLHGDMVNFGQLKYRFMLR
                     TPPPPRTPRITPVTPED"
     gene            8453..9316
                     /locus_tag="PLR93_00395"
     CDS             8453..9316
                     /locus_tag="PLR93_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNH25442.1"
                     /translation="MIRTTLAHLHIAALSHAGISGKNNEDRYAVSSFQLSKDDPRPST
                     FAVVADGIGGHRAGEVAAELAVNYISMHIAESNARKPIKILENAIHDASQAIATHSAG
                     KAEEQGMGSTCACVWIVENKLYTAYVGDSRIYLLRGKYIQQITIDHTWVQEAYEKGII
                     TADQMRDHPNVHVIRRHLGGIRLPEVDFRLRLDNNETEEESLNNQGFHLQPGDTILIC
                     TDGLTDLVWDDEIKDIIRGKHDLKTAAETLVNLANERGGHDNITVVLLAMPRVEETLP
                     KKSILGWLTGE"
     gene            complement(9377..>9778)
                     /locus_tag="PLR93_00400"
     CDS             complement(9377..>9778)
                     /locus_tag="PLR93_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25443.1"
                     /translation="WFVIQRGRVSLLGFCLRRSCGYSIFETWHFGWQWGLATAGGSVL
                     ALNPLAFVICLGLLILLVLVVRHSARASVFAGILFAPVLWLLSIRDVAFWVAVGAGAV
                     IAVRFLIDWNRKYRELWLDREKKSEKVEKAD"
BASE COUNT         2398 a         2574 c         2238 g         2468 t
ORIGIN      
        1 atcttgaaaa cagaccctaa tgggagttga ccctgagcag cacggttcag aagcgggttt
       61 ctcgtgtcat taaaaatatt ccctacagct tggttattgc ggttattgct atcaaaaaca
      121 ttcgggtcaa aatcaggtcc cgatgccata gcgatgatac gcccggtatc gacttccatc
      181 actacaatgg caccacggaa atattccact gccgcctgag cgtagtattg catattgcta
      241 tcgagagtga gatataccga atcagcgggc gcaggttgac tttgccccaa ggtggctatc
      301 acctgccccg ttgggctaac aacacgtaaa atgccgccat gtttgccggc taaataatct
      361 tctgcccatt gctcaacacc ggtataacca attcgctcac taccactata gcctaaacgc
      421 ttgtattgat taagttgttc tggagaaatc gccagggtgt atcccacggc ttgcggagcc
      481 aacccaccgc ggaagtaata gcgtgagtta tattcttgaa tgaccaaacc accagggtta
      541 atagagagta aacgctgggc ttcttcacgc gttccttcac acatcggcag ataccaaccg
      601 ggtccggcag catcgatctg cgcctgtata tcaagcgggt ccaagccaca taaacgccca
      661 agttcggttg tcaatgcgcc gatcatgtca tagtcaattt gatcggtttg aatcccaaaa
      721 gcaaacgcat cagattgagt ggcaattggc aagcctttgc tatcgtagat atccccacgg
      781 gcagggatgc tatattccat ctttagtaag ttgccaccag ccaattcagg aaggatcagg
      841 gcgtcgtccc attgcacttt ccaattgcta tcttcattcg taaggcgcat caacatttca
      901 cgttgaatgt cgccaacaag ggcagtctta tacgtaatac tgtatttaac ttctgcatta
      961 aaaggcgtaa tggattttga gagaacggtc acatcaaaac tggatgcgct catttcattt
     1021 agagcattcg tccaacgctt tgagaaatct tccaaggtca acgcatcgcg gctggactgt
     1081 gacaacatgg catacatttc ttcgtaattg tcacctttta acgcatcaaa aaacgcagtg
     1141 actgctgaat ccacgttagg tgcgggagtg attccgaccg acggggttgg caagggtgta
     1201 tcggttgcaa aaatcgaacc gactccactc cctgagccag gtccggtgca gccgacaatg
     1261 attaaagcaa cgagagctaa ttgtttccag cgtaatgact tcattacgtt tcctttcaat
     1321 ttctaattga cgatctcacc gctcaagatc aacggcgaga ccttacaatc atactgtaaa
     1381 agagattggc atgcagatgg aacatctgca aaagatgaaa tattaaagtg tttcaaagtg
     1441 cgcaccacat gacacatagg caaacccatc accccggcac ggcaaccttc caatgaagag
     1501 accggcttaa actccgggtg ctgaatggca tatgcaccag ctttatccat ggggtcgccg
     1561 gttttgatat aggtttcaat ttcttggtct gagtattcgc gcatgggcac atcaatcatg
     1621 gacaagtctg tatacatctt tccatgttga aggatggcaa tcgcggtgta gacctgatgc
     1681 gtccgcccgc gcaattgctt taacatgcgc cacgcatcag attcatcgac aggtttcccg
     1741 aggatctcgt tcccatctac caccgtagta tctgacccca gaataaccgc atccagcccc
     1801 gccttgggtt gaaccgctga agctttggct tgtgccaatc tgatcacata atcccgtgga
     1861 ggctcatgct caaacggagt ctcatccaca tctgcaacga tgacggtgaa ctcccacccg
     1921 cccaagccaa atagttgtcg cctgcgcgga gagttagatg ctaaaacgaa ctttacttta
     1981 tccatcaaga gcaagattct aacacagtca tttttgatgg attaaaccgc gataagtcgt
     2041 tggaaatatt ggcaaagtac tcagacaact ttttttgtca tttatcttct ctcttttcaa
     2101 atccggttgt aaaatcattc gatatctgtc cagacaatcg gaggcttcat gaatgctttg
     2161 caagaacgaa ttttaaaaga ggggaaagtg ctcggcggtg gaattcttaa agtagatagt
     2221 tttgttaatc accaagtaga ccctgtgctc atggatgagt gcgggcgtgc gtttgccaaa
     2281 ctctttgcca atgtgggtgc aaccaaaatt ctcacagcag aaatctcggg cattgctccg
     2341 gcgctcacca ctggcattca catgggtttg cccgttgtgt atgctcgcaa gaccaaaccc
     2401 gttaccatgc ccgatcaggt gtatctcacc ctcgcccctt ctcacaccaa aggtcgcatg
     2461 gtggagttga tcgtctcacc ggaatatctt gccaacaacg aaaaagttct catcatcgat
     2521 gacttcctcg ccagcggaca gaccattctt gggcttgtac gtttggcaga agcctctggg
     2581 tccaaagtcg ttggcattgg cgcgttggtc gaaaagagtt tcgaaggcgg gcgtgatgct
     2641 ctcgcttctg ttggtgtgca aattgaatcc atcgcttgta tcaagtcact ggatgatggc
     2701 aagattgagt ttgtctaaca cacacggttc actctttgca aagagtgaac cgtgattcga
     2761 aaaatcctac acctgcgctt cattgttttc ctcatcggtt ggatcttcgc tcacatgagt
     2821 ttcgccatcc cggtcaaacg cctccgtgag acctcgaacc tgattgcgtt tcagcatcct
     2881 tctcccagtt ataaggttca tttgcttatc accccgaagc gacaggttca atcgttggca
     2941 gagcttgacc cacaagacac cgctttcttt acagacttat actccactgt gcaaagcctc
     3001 gtaaaagaac ttgacctcaa agcgtatcgg ctaatcgtca atggcggaga gtatcaagat
     3061 tttccacatt tacattttca tcttatttca gaaatgtagg ggtgagtccc ccctacggga
     3121 ttaataaaac acatgaattc aatcgccatt ttagatttcg gttctcagta cgcccaaatt
     3181 attgcccgcc gtgtgcgcga gtcacaagtc tattgtgaac tctttccatg ggatgcgcca
     3241 caagaaaaaa tcctttcgat aaaaccgaag ggatttattt tatctggcgg acccaagtct
     3301 gtttatgaag agaatgcacc cttcattcaa aaattcatcc tcgaaagcgg gcttcccatc
     3361 cttggcattt gttatggcat gcaagccctc acccacgccc tcggcggcaa agtggaccca
     3421 tctgcccagc gcgagtatgg tcatgccgaa atcacgccat tggttcccgg caccatgttg
     3481 gatgtgctct ccaaagtttg gatgtcacac ggcgataaag tgacacaact ccccgaaggt
     3541 ttcatggcac tcgccaaaag cggaaacagt cccttcgcgg cgatgggcga tatgaaacgc
     3601 aaatattttg gtgtgcaatt tcaccccgaa gtgcatcaca ccccgaacgg caccgaactc
     3661 atcaagcact tcgccatcga catttgtggc gtaacgcccg attggacccc cgctaacatc
     3721 atcgaagaaa gtgtaaaacg aattcgtgag caagtgggcg atgaacgtgt gctcgcagcc
     3781 gtttccggtg gcgtggattc ctctgttgcg gcggcattag ttcataaagc gattggcgat
     3841 caactggtct gtgtctttgt ggatacggga ctcttgcgcg ccaatgaagg cgcacaagtc
     3901 gcttcggcat ttcgggaagg cttgggtgct gaactcatca ccgtcgacgc cgctgacgat
     3961 tttctcggcg cattgaaggg gcaaactgac ccagagatga agcgcaaaat tgtcggcgag
     4021 aaatttatcc gcacctttga acgtgaagcc aagaatgtag gtcaacctag attcttagtg
     4081 caaggcacga tctaccccga tgtagttgag tcatccggtc ccgaccgcag caaagcagcc
     4141 aagatcaagt ctcatcacaa tgtgggcgga ctccctgaag atatgcaatt tgaacttgtg
     4201 gaaccgttgc gctatttatt caaggacgaa gtccgtttgg taggtgaagc cctcggcttg
     4261 aattccagtt tggtgtggcg tcaaccgttc cccggtcccg gtctgaccgt gcgctgcctc
     4321 ggggagtgta cgcccgagcg tgtggcacgt ctgcgggcgg cagatgcgat ccttctggag
     4381 gaattatcca aagcaggttt tctgggcaag ggcgctcaaa cgtcacaggc atttgttgtg
     4441 ttactcccgg ttcgttcggt gggcgtgatg ggtgacatgc gaacctatca agaagcagca
     4501 gcgatccgcg cggtagcaac ggatgacttc atgaccgccg attgggctcg cctgccattt
     4561 gaattattag caaaggtctc gagccgtatt gtcaatgaag tagaaggcat taatcgtgta
     4621 gtctatgata taacgagcaa accgcctgca accattgagt gggagtaaat caattttcga
     4681 ttgacaattg ggagatggaa atgaacttcg actatggcaa tatcttaacc cgcgcattaa
     4741 agctcacctg gaagcataaa tcgttttggc ttctcttgat gattcccgtg ttcatttctt
     4801 tcatgattct tgcaacaatc attttgccgg tcatcttttt aactgaaaac gaagaatcta
     4861 tgaccttgat catggtgctt tgggctggga ttgtcatttt tggttttttt gctagtttta
     4921 ttatcggcgc agttagtatg aatgcagtca cacttggcat tttgcgagcc gagcgcggtg
     4981 aaggttctac tgcgcttatg gatgtagtcc gcgatagttt tcagtatttc aagaatgctt
     5041 ttggtgcagt tcttatcgtc caactcagtg tgggtgccgt atttacagtt ttctttctct
     5101 gtatgacggc tctcaccctt gtcaccatgg gtattgcatc catctgccta caaccgatca
     5161 tgcttttgct cactccccta tcatttttag tgggtgccgt cctctatgga gcgttggtag
     5221 cggtcattga tgaagggctg ggcgcattgg atgcagtcaa acgtgccgtg caagttgtcc
     5281 gtgatcatgt gtggaagttt gtcgtcattt cgttgatcgc ctatttcgga tccatgattg
     5341 tctccagcat tttcaccttt cctctcatga ttccagccat gttcggaccc gctctcattg
     5401 aatctggggt ggacatcacc ggaaacacct ttgctctcat ctttattcca tttctttgca
     5461 tatttggagt actgatggcg ttcgtctctg gtgtaacggg gacttttata gccgcctcca
     5521 tggaaatttc gtacctacaa ctctctcgcc ctgtcccaga agtcatcttt gccgccgatg
     5581 ccccaaaaga tgcgacttca taatcaccct ccacgtacat aaggatagaa cggcgcagac
     5641 cgttcactgt cacctcccaa gtggacggaa acctttgagt agtgtatgat tcaagcggtt
     5701 tcgcctcggg agaaaccttg taattttgga gaatgttatg aaaatcgatc ttggaaaaat
     5761 acttacccga tcctggcaga tcatttggaa ctataaagtt ctatggatct tcggtatttt
     5821 ggcgggtttt gcgagcggga atgggggctc aaatggaaat tccaattcct ctaatcgcgg
     5881 ttcttcttat tcaggcaata gcgaccagtt tgttcaacaa gcgaaagaat ttttccagca
     5941 atacatgctc atcatcattg ctgtttgtgc tgggctagta ttgctctctc ttgtctttta
     6001 tgccctcggt atgatgggtc gcattggtat tctaaaaggt gtctataaag tagaagcagg
     6061 tgcaagttct ctcatgtttg cagaactttg gtctgaaagc atgccttann nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cgttaagtct gatattggcg gcatgatcgt
     6241 tctttcgcta gtgctcggta tcggcggcgg aattatcggc ttgatcattg ccatccctgt
     6301 gattattgca attgtgcctt ttgtactcgg tatggcggca aacaacttcc aaatgggcaa
     6361 ctccatcccc gccacggcat gggtttcgtt ggcttgttgc ggtttgtact tcccggttct
     6421 cttggtatta aatggtatcc ttacagcata tgtgaaaact tcctgggcgc tctcgtattt
     6481 gcaattgagc caacccaagg aaaatccccc tgtccttgcg caggataatg cgtaaactat
     6541 tcctaaccct tctatcaatt agccttttgt tcggcgtggt gatcgtccct gcccaagcgc
     6601 aggggtcgat caccgcgtcc gttactattt acaacgtcga tgcgtccaac ttcccgtctg
     6661 tgaacggatt catggacgtg acggatgcca acggaatctt cgcctcaggg ctcaagccgg
     6721 aagctgttac ccttgtcgag aacggacagc ccctcccagc cgattcgttc aacgaactag
     6781 cgattccgct tcaattagtc gttgctttca atcagggtga agccttaggg gcacaaaatg
     6841 caaatggcat ctcccgctat caacgtgtgg aacagatcat tgagcagtgg gcacaaaccc
     6901 gcccagcaga cctgcccgat gacctaagcc ttgtctcgca agcgggtccg gtcatcaacc
     6961 atgccccgcc agcagatttc gtagtcggct taaaaggctt ccagccagat ctgcgcaccg
     7021 ctactcccaa catccaatct ctggtcagcg cactagatgt ggtcagcgcc caaacaccgc
     7081 gcatcggtgt gaagcgtgcc attctattca tcacaccgca aatgacggac cccaacctcg
     7141 ccgcatcggt tgagccgctg atccaacgcg cccttgaaaa taaaattcgc gtcttcatct
     7201 ggtatgtgga cgcaaacaca accttcacaa ccaccagtgc tgccgtcttc aacaaccttg
     7261 ctatccaaac cggcggcacc atgttccaat tctcaggcga ggaacgcttc ccagaccctg
     7321 aagaatattt ctctccgttg cgtagaattt acaatttctc ttacacctcg cgtgtcaaca
     7381 cctctggcaa ccatactgta gcggttcaag taaatctgcc ttctgttggt ccgctgaatt
     7441 cagagacact tcctttcacc ctcaacatcc aaccacccaa tgccttcccg gtcgcgccgt
     7501 cgatacagat tacgcgccaa gcgcctgaag aagacccctt caacaccgaa gtccttttgc
     7561 cagaaacgca agagatcgaa atcatcatcg aatttccaga tgggtttgat cgcccgcttg
     7621 ctcgcaccac tctgatcgtg gatggcattg ccgtggatga aaacacagcc gagccattta
     7681 caaaatttac ctgggacttg aagccttaca caattagcgg tgagcatcaa ttgaccgttg
     7741 aagccgtgga ctctctgggt ctcagtaaga ccagcatgcc tttgccggta gtggtaaccg
     7801 tcatcaaacc gcccagcgga cctgctgcct tccttgcaaa atatcgcatg ccattaacct
     7861 tcggcgcgat cattctcgct gggcttgtct tattcgccat cctgctcagt ggacggttac
     7921 gcatccgttc gttgcgggtt atgcaagaag aacgccgcgt tcaaaacgac ccattgaccc
     7981 agtctcttga agcgtttaca gaaccggctc cggtggcggt gaaagaaaaa actaaaaagc
     8041 cacgcaccaa gtccacgaag gcgaaagcag ccaaaacgtt ggagaatgag gcgttggctt
     8101 ctctcgtccg cctccaagcc gatggacaag tggcggctgt cccgcctatc caattaaacg
     8161 atgcagagat cgtttttggc accgaccctg tgcaatgctc gcaaattttg gatgacccct
     8221 ctatttcgtc tgtccatgcg cggattcgcg ccacagaaga tggcggttat ctactgatgg
     8281 atgcccagtc gctcgccggc acctgggtca attacgagcc tatcccccgt gagggctacc
     8341 ggttactgca tggggatatg gtaaactttg gtcaattaaa gtaccgcttt atgttgcgga
     8401 ctcccccgcc accgagaact ccaagaatca cccctgtgac tcctgaagac taatgatccg
     8461 taccacactt gctcatttgc acatagccgc tcttagccat gcgggcatct ctggcaagaa
     8521 caatgaggac cggtatgccg tctcgtcgtt ccagctcagc aaagatgacc cacgcccttc
     8581 aacgtttgcc gttgttgcgg atggtattgg cggacaccgc gcgggtgaag tcgccgcgga
     8641 attggcagtt aactacatca gcatgcatat tgctgagagt aacgctcgca aacccatcaa
     8701 aatcctcgaa aatgccattc acgatgccag ccaggcaatt gccacccact ccgcgggcaa
     8761 ggcagaagag caaggcatgg gctctacttg cgcctgtgtg tggattgttg agaacaaact
     8821 ctacaccgca tatgtaggcg actcgcgcat ttatcttttg cgcggaaaat acattcagca
     8881 aatcaccatc gaccatacct gggtgcagga agcgtatgaa aaaggcatta tcactgccga
     8941 ccaaatgcgc gaccacccca atgtgcatgt cattcgtcgg catcttggcg gcattcgcct
     9001 gcccgaagtg gacttccgcc tgcgcctcga taacaatgag accgaagaag agtcgcttaa
     9061 caatcaaggg tttcaccttc aacccggtga caccatactg atctgtactg atgggctcac
     9121 cgacctcgtt tgggacgatg aaatcaaaga catcatccgc ggcaagcatg acctaaaaac
     9181 cgcggcagag accttggtca acctcgccaa tgaacgcggc gggcacgaca atatcacggt
     9241 cgtcttactg gcaatgccgc gagtggaaga gaccctgccc aaaaagagca ttcttgggtg
     9301 gctaactgga gaataaaaaa ggacggtaac cccgtccttt ttttacactt ctttcttagc
     9361 cgatcatggg tccgtcttag tcagcctttt ctaccttttc gcttttcttc tcccgatcca
     9421 accacaactc acgatactta cgattccaat caatcaaaaa gcggacggct atcaccgccc
     9481 ccgcccccac tgccacccaa aatgcaacgt ctcgaatact gagcaaccat aaaaccggcg
     9541 caaacagaat cccagcaaag acactcgccc gcgctgaatg acgaaccacc aagacaagaa
     9601 ggatcaataa tcccaagcaa atcacaaacg ccagcggatt caacgcaagc acacttcccc
     9661 ctgccgttgc cagcccccac tgccacccaa aatgccacgt ctcgaatatt gagtaaccac
     9721 aagaccggcg caaacagaat cccagcaaag acactcgccc gcgctgaatg acgaacca
//
LOCUS       DAOQFT010000009         9755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3256_length_9755_cov_6.725464, whole genome shotgun sequence.
ACCESSION   DAOQFT010000009 DAOQFT010000000
VERSION     DAOQFT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_9755_cov_6.725464"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLR93_00405"
     CDS             complement(<1..452)
                     /locus_tag="PLR93_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH25444.1"
                     /translation="MPDTSLYLHIPFCKHRCAYCDFNTYAGQESLIPAYVEALIHEIE
                     FIGNQQPDFKVPTIFFGGGTPSLLSGPQFDSIMSALSSAFALSPTAEVTIEANPGTIS
                     PEKLEAIRKAGINRISFGVQSSVTEELRMLERSHDFFTVIEAVSTARKA"
     gene            complement(485..1291)
                     /locus_tag="PLR93_00410"
     CDS             complement(485..1291)
                     /locus_tag="PLR93_00410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH25445.1"
                     /translation="MENPLEHLHNETHQAWEANAEVWDKRMGDEGNDFFNLLCWPALA
                     SFLPSKSDAHILDIACGNGLTTRRLAEMGYQVTAFDFSANLIEYAKQRLDKHGSKTSL
                     HVIDATNEAQLLSLGKNKFDCALSNMALFDMANIEPLFQTLPQLLKPGGTFIFSITHP
                     CFNNASSMHVVEEMDDGEIKTIYAIKTSRYMTPYSMRGLALRNQPKPQVYFERPLQYY
                     LNLGFKNGFVLDGFEERAFPPETPQTTPLGWGGKFSEIPAALVIRMRLNS"
     gene            1604..1987
                     /gene="speD"
                     /locus_tag="PLR93_00415"
     CDS             1604..1987
                     /gene="speD"
                     /locus_tag="PLR93_00415"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNH25446.1"
                     /translation="MNASLKLGEHYIFDLSDCNREILMDSEKAYSLFAQAVRDSGLTV
                     VDEGFYKFSPHGFTAFLLLAESHASLHAWPEHNYCAIDLFTCAIGQDFMPVLMRLKEA
                     FGADDFSVRKLDREASVETKMPIAM"
     gene            2061..2999
                     /locus_tag="PLR93_00420"
     CDS             2061..2999
                     /locus_tag="PLR93_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNH25447.1"
                     /translation="MSESKPPIYWISDYLTPDDILNHAVHKVILHKHTPFQEMWIVDC
                     GTYGKGLILDGIWQSSTGDDFLYHESLVQPACVNHGDPKRGLVLGGGEGATIRELLKW
                     KNMERVVMVDLDREVVEACKEHFPEMHQGAFDDPRVELVIQDALAYLDSSPSEWDVII
                     SDLTDPIEHGPSFMLFTKEYFAKAQKALRPGGYFLLQAGPVSPPSMDMHIRLVSTLKA
                     VFKYVHSYHIHVPMLGHPWGFILAGDTPIDVRPAPEMVDAILADKTNGKFKMFDGITL
                     LGLMQSPKHIRDAIAKETTVYTIEHPPVFSTQELPK"
     gene            3073..3903
                     /gene="speE"
                     /locus_tag="PLR93_00425"
     CDS             3073..3903
                     /gene="speE"
                     /locus_tag="PLR93_00425"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNH25448.1"
                     /translation="MSIWFTEELHPYYRKGIRVKRVLADEQTKYQHLQVVETEFFGNA
                     MILDGIIQLTERDNMGYHEMIVHVPMLAVEKPKRVLLIGGGDGGSLQQVLRYPSVEEV
                     SICELDQRVVDLSREHFAASFGDPWADPRSKLLIRDAFGYLEENPGTFDVIISDTTDP
                     IGMAERLFSDEFYKLMVRALAPGGAAATQCEQPFFDTELIKTIYQTAKSLTKNPAYYY
                     TNVPTYPGGGIGFMYVSNTPWENGLKTPYPPGVNQYINPEIHKAAFALPEFFRKELYG
                     "
     gene            4003..4965
                     /locus_tag="PLR93_00430"
     CDS             4003..4965
                     /locus_tag="PLR93_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HNH25449.1"
                     /translation="MNPIKGLHHVTAVARDPQRNVDFYRNVLGQRLVKRTVNFDSPDT
                     YHFYFADETGTPGSVLTFFAWPNMRRGVRGNGETNTVAYNVPVGSLSFWEKHLKQNGI
                     VPGNIQSRFNQNVLPFTDPDGMNLELVEVENLPAIQHWEDGPIPREYALHGFHSVTLW
                     VDEAEPTASLLTEGMGYRFAGAEEDRQRFKGDTNALGHIVDVVERPQKMPAGFGAGSI
                     HHIAFRVPTDDEQLKYQSILGEAGFRVSPVMDRSYFHSIYFREQGGVLFEIATDTPGF
                     MVDEPKDLLGIKLKLPAWVEPNRDEIEASLLPLTLNPIEKASLS"
     gene            4962..5597
                     /locus_tag="PLR93_00435"
     CDS             4962..5597
                     /locus_tag="PLR93_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25450.1"
                     /translation="MTIPHETSPVYAAGAKLEEAKSAMILLHGRGASAQDILSLSTYL
                     NFPGMAYLAPQAEGSIWYPNRLIAPVESNEPYLSAAIAKIDGLVKHVEAGGIPVEKIF
                     IGGFSQGASLSSEFVIRNPRPYGGLLIFSGGYVWQSGKPRTAEGNLKGVPVFNGCSNV
                     DPHIPLERVKETTALLTSMGAEVNEQIYLNMDHTIVDEEIEQARALVEKRL"
     gene            5744..8971
                     /locus_tag="PLR93_00440"
     CDS             5744..8971
                     /locus_tag="PLR93_00440"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNH25451.1"
                     /translation="MLGVTLSLLLSLLVPSGWVSARLEQALGTISFVPNIETAGVVVS
                     GTGLPATAQLFYRRAGDVNWQPGHALLRIKDGRLIGSLFNLAASTSYEVRVSDGVTEV
                     SGSFTTQADELLFAPTNIIYVNASAQAGGDGSASAPFRTIQEGVNRAAPGTQVLVADG
                     IYSESVTFPASGTAGSWIQVKAQGGGAILDGSASVLATDWNPVEGKANVWFTKISPGI
                     KYLARDGLRFYQYDFLSDLYDQRGHNNVPMSEGWFYDPINGRLYVRINREPHKYTWQV
                     PKFNAAFMIDGRDWIWIEGFEMRYYGTEFGCGVCSKNSSHVVIRKNKIHHIQNPVFVE
                     WTGGEDRGNDTRIEYNEMYDTLTGGDWAWGAIKSTAMESMAIVLRGHTGAIVRGNTIH
                     HYFNGIYTSSSAALDNPGVAFDADIYNNYVYHIGDDAFEPEGTAVNHRFRNNKVDSTL
                     VGISLAPVTMGPVWIVRNTFTNFTARSVKWDRGSDGWVLMYHNTSFTTYTNTHSVELI
                     SAVKNSILRNNIFHANGYSVEAHNPGSTGHDWNYNDWFALNSNAPFMWEGISYATLNQ
                     WCRSTGLDCNGYEDSPGLSTDMTLLSNSPNIDRGVLIPGINDQFMGSAPDLGAYESVY
                     GTSPATPVATNTPLPLPTDTPTPMPVYSSPVVTSITLADPTPNNAELVRFFVNFSEPV
                     NNVDVNDFMMTATGSISNYTILDAYPVGSAYAITVYTGVGDGTLRLDLVDNDSILSAT
                     SMPLGGVGIGNGNFSLGNVYVINKNAPFVTSILRADPSPSNAGSVRFSITFSESVSGV
                     DVADFVLSTSGLSEAGIASISGSGTAYMVTVNTGIGSGTLRLDLNDDDSVVDSAGAPV
                     GGIGVGNGNFAAGETYTIDRNIPLIETVSFSSDGAQDGWVMESKETSNKGGTLNSNAI
                     TFRVGDNAQDNQYRSILQFSTGALPDNAIITQMILTMQLETIVGTNPFTTHRNMWVDI
                     RQGAFGSFGPLQIGALQISDFQAPASLYNAGTIQNNAVGGWYWVNFDSGVFSLVNLKG
                     ATQFRLGFQLDDDDDRKDDYLSFFSGNYSVQSARPQLTIKYYIPK"
     gene            9082..>9755
                     /locus_tag="PLR93_00445"
     CDS             9082..>9755
                     /locus_tag="PLR93_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNH25452.1"
                     /translation="MTGIFISYSRKDSQAAKKLMEGFKSVKLDVWVDWEDIPPAVGWL
                     DQILHGIEQAEVFVFLISPDSIKSEVCKVELEHARKNSKRIIPVVARDVVVQDVTPIL
                     RDLNWIFIREQDDFAAGLEKVNLAINLDIEWLQEHRRLQVRALEWDRKKESSLLLRGT
                     DLRNARKMIVANEKKDPFPSPLQKLYIESSRRGEQLRLTTYVSAAVTLFIMVFLSLVA
                     LNQRQEA"
BASE COUNT         2420 a         2195 c         2484 g         2656 t
ORIGIN      
        1 gcttttcggg cagtggatac ggcttcgatg acggtgaaaa aatcatgaga gcgttcaagc
       61 atgcgcaatt cttctgtgac agaagattgc acaccgaagc taatgcggtt gatgcctgct
      121 tttcgaatgg cttccagttt ttctggcgag atggtgccag ggttggcttc gatggtcacc
      181 tctgccgtgg gggacaaggc aaaggcggaa gataaagctg acataatgga atcaaattga
      241 ggtccagaaa gaagcgaggg ggttcctccg ccaaaaaata tggttggtac tttgaaatct
      301 ggttgttgat ttccgataaa ttcaatctca tggattaagg cttcgacgta agcgggaatc
      361 aaagattctt gcccggcata ggtgttgaag tcgcaatagg cgcagcggtg tttgcagaaa
      421 gggatgtgga ggtagaggga ggtgtcgggc atttgatatg ggttgagtat aaccgccttg
      481 aaggctaaga gtttagccgc atgcgtatga cgagtgcggc tgggatttcg ctgaacttgc
      541 cgccccagcc aagcggtgtg gtttgcggtg tttctggtgg aaacgcacgt tcttcgaagc
      601 catcgaggac gaagccattt ttgaaaccaa ggtttaagta gtattgcaac ggacgttcaa
      661 aatagacttg cggtttgggt tggttgcgaa gagcgagtcc gcgcatggag tagggtgtca
      721 tatagcgcga ggttttgatg gcgtagatgg ttttgatctc gccatcgtcc atttcttcta
      781 cgacatgcat ggacgaagca ttattaaagc aaggatgggt gatggagaaa ataaaagttc
      841 cacccggttt aagcaattgc ggcagggttt gaaagagcgg ttcgatattt gccatgtcga
      901 agagcgccat attggagagc gcacagtcga atttattctt gccaagagag agaagttgag
      961 cttcgtttgt ggcgtctata acatgcaggg atgtttttga gccgtgcttg tcaagccgtt
     1021 gtttggcata ttcgattaga ttggcggaaa agtcgaaagc ggtgacttgg taacccattt
     1081 cggcaagtcg ccgggtggtt aagccatttc cacaggcaat atccagaatg tgggcatctg
     1141 attttgaggg taggaaggag gcgagagcgg gccagcacag cagattgaaa aaatcattgc
     1201 cttcgtcgcc catgcgtttg tcccatactt cggcgttggc ttcccatgct tggtgggttt
     1261 cattatgcaa atgttccagc gggttttcca ttgaacctct taaagatttg aaatggtctt
     1321 gcggcttatt ttatacgtga atcaaattgt ttatgtaggc ttgctttgag tcatgattat
     1381 gacctgtatc ttgcttgcaa tgatgatttt gatttcaaca ggctttgaca atgaaaaata
     1441 ttcactggtg aaaaagattg acactacacc agtccatagt ataaatcggc gcaacagagt
     1501 ctacttaagg acggtctcaa ccgttacgca gggaaacatc ctgcggatcg tttgggaaag
     1561 gttcttgggt gggttcttta attttttaac accggaggtg tgaatgaacg ctagtttgaa
     1621 attgggagag cattacattt tcgatctctc cgactgcaac cgtgagatcc tcatggatag
     1681 cgagaaggct tactcgttgt ttgctcaggc tgtgcgcgat agcggtctga ccgttgtaga
     1741 cgaaggattt tataaattta gcccgcatgg ctttaccgct tttcttctcc ttgcggagtc
     1801 gcatgctagc ctgcatgcct ggcccgagca taattattgc gccatcgact tatttacctg
     1861 tgctattggg caagacttta tgcctgtttt gatgcgcctc aaagaagcct ttggcgcgga
     1921 cgacttctct gtgcgcaagc tcgaccgtga agcgtctgtt gaaaccaaaa tgccgatcgc
     1981 gatgtagtta agtggtctat cttctatcgt tttgatatac tccgtagatc taaattattt
     2041 tcattcacca aggaaaaaga atgtctgaat caaaaccccc gatctattgg atctctgact
     2101 acctaacccc tgatgatatt ttgaaccatg cagttcataa ggtcatcctg cataaacaca
     2161 ccccattcca ggaaatgtgg atcgtggact gtggcaccta cggcaagggt ctcatcctcg
     2221 atggcatttg gcaatcgtcc actggcgatg atttcctcta tcacgagtcg cttgttcaac
     2281 ctgcctgtgt caatcacggt gaccccaagc gcggactcgt ccttggcggc ggggaaggtg
     2341 cgaccatccg tgaattgctc aagtggaaaa acatggaacg cgtcgtcatg gtcgaccttg
     2401 accgtgaagt ggttgaagcc tgcaaggagc atttccctga aatgcatcag ggtgcgtttg
     2461 acgacccacg cgtggaattg gtcattcaag atgcacttgc ctatctcgat agctctccct
     2521 ctgaatggga tgttatcatc tccgacttga ccgaccctat tgaacatgga ccgtctttca
     2581 tgttgttcac caaggaatat tttgctaagg cgcaaaaagc acttcgcccc ggcggttact
     2641 tccttcttca agcgggtccc gtttcgccgc cttcaatgga tatgcacatc cgcctcgttt
     2701 ctacgctcaa ggcggttttc aagtatgtgc attcctatca catccatgtg ccgatgctgg
     2761 ggcatccttg gggctttatc cttgctggtg atacgcccat cgacgtccgc cctgcaccgg
     2821 agatggtcga tgccattctt gccgacaaga ccaacggcaa attcaaaatg ttcgacggca
     2881 tcactttgct cggtctgatg caatcgccaa agcatatccg cgatgcgatt gcaaaagaga
     2941 ccacagttta caccattgaa catccgcccg tattcagcac gcaggaatta ccgaaatagt
     3001 agtaaagacc taagccgtta caagaccgcc gacaacagaa tggtcggcgg tctttacttg
     3061 aaaggataac gaatgagcat ttggttcact gaagagttac acccttacta tcgcaaaggc
     3121 atccgtgtca aacgcgtgct tgccgatgaa cagacaaaat atcaacactt gcaagttgtc
     3181 gaaaccgaat tttttggcaa cgccatgatc ctcgatggca tcatccaact gaccgagcgc
     3241 gacaacatgg gctatcatga aatgatcgtc cacgtgccca tgctggcggt ggagaagccc
     3301 aagcgcgtgt tgcttatcgg cggcggcgac ggtggctcgc ttcaacaagt cttgcgatat
     3361 ccttccgtcg aggaagtttc tatctgcgaa ctggatcaac gcgtcgtgga cctctcacgc
     3421 gaacacttcg ccgcctcttt cggggacccg tgggctgatc cacgctccaa gctgctcatt
     3481 cgggacgcct ttggctacct agaagaaaac cctggcacct tcgatgtcat catctcagat
     3541 accaccgatc ctattggcat ggcagagcgt ctcttttccg atgaatttta caagctgatg
     3601 gtacgtgccc tagctcctgg tggtgccgct gcgactcaat gcgagcagcc tttctttgac
     3661 accgagttga tcaaaaccat ttatcaaacc gccaaatcac tcaccaagaa tcccgcctat
     3721 tactacacca acgtccccac ataccccggc ggtgggatag ggttcatgta cgtttcgaac
     3781 actccttggg aaaacggttt gaaaacgccc tatccacccg gtgtgaacca atacatcaac
     3841 ccagagatcc acaaagcggc gtttgctttg ccagaattct tccgcaaaga attgtacggg
     3901 taaaataaga ccaagcctcc aaaattaact ttggaggctt ttcatacgaa attcaaacgg
     3961 atatttttgc gatagagtgc gtctaggcaa taggagagaa atatgaaccc tattaaaggt
     4021 ttacatcatg tcacagccgt ggcacgtgac ccacaacgaa atgtggattt ttatcgaaat
     4081 gttttagggc aacgcttggt caagcgcaca gttaacttcg actcgcccga tacctatcac
     4141 ttctattttg cagatgaaac cggcacaccc ggcagtgtat tgaccttctt cgcttggccc
     4201 aatatgcggc gcggcgtgcg cggcaatggc gaaaccaata ccgttgcata taacgtcccg
     4261 gttggttcac tttccttttg ggaaaaacac ctgaaacaaa acggcattgt ccccggcaat
     4321 attcaaagcc gctttaatca gaatgttctt ccttttacgg accccgatgg catgaacctg
     4381 gaattggtgg aagtggaaaa tttacccgcc attcaacatt gggaagatgg acccatccca
     4441 cgtgaatacg ctttgcatgg atttcatagc gtcaccttat gggtggatga agccgaacca
     4501 accgcttcac tcttgaccga aggcatgggc tatcgcttcg ccggggcaga ggaagaccgc
     4561 caacgcttta agggtgatac gaatgccctc ggtcacattg tggatgtggt tgagcgcccg
     4621 caaaagatgc ctgcagggtt tggggctggt tccatccatc atattgcttt tcgcgtgccc
     4681 acggatgatg aacaactgaa atatcagtcc atcttgggtg aggcggggtt tcgcgtaagc
     4741 ccggtaatgg atcgcagcta tttccattcc atttattttc gggagcaagg cggtgtgttg
     4801 tttgagatcg caaccgacac acccggtttc atggtcgatg aacccaagga tttgctgggc
     4861 ataaagttga aactgcccgc ctgggtagaa cccaatcgag atgagatcga agccagctta
     4921 ctgccattaa cccttaaccc tattgagaaa gcgagccttt catgaccatt cctcacgaaa
     4981 catcccctgt ctatgcagcg ggtgcaaagc tagaagaagc caaatctgcg atgatcctcc
     5041 ttcatggacg tggcgcatcg gcacaagata ttctgtccct ttccacatac ttgaactttc
     5101 cgggcatggc gtatctcgct ccccaagcgg aaggatccat ttggtacccg aaccgtttaa
     5161 tcgcgcctgt tgaatccaat gaaccgtacc tttcagccgc catcgcgaag atcgatgggt
     5221 tggtaaaaca tgttgaagcg ggcggtattc ctgtcgaaaa aatatttatt ggcggtttct
     5281 ctcaaggcgc cagtctttcc agcgaatttg tgattcgcaa tccgcgcccg tatggtgggc
     5341 tgttgatctt tagcggcgga tatgtttggc aaagcgggaa accccgcact gcggaaggca
     5401 acttgaaagg tgtaccggtt ttcaacggct gttcgaatgt ggatcctcat attccgttgg
     5461 agcgagtgaa agagaccacg gcactcctga cttccatggg ggcagaagta aacgagcaaa
     5521 tttatctaaa catggaccac acgattgtgg acgaagagat cgagcaagca agagccttgg
     5581 ttgagaaaag gttataagaa atcgccccga aaggggcgtt ttttgtcatg gtaccagaat
     5641 tgtattttta taggtgcgtg ctatagtgaa aacagacagg gttttgtttc catccctgta
     5701 aatggttttt atgaacacgc atggtagtat atcgaaattt atagtgctgg gtgtaaccct
     5761 ttcgctcctg ttatcactgc ttgtgccgag tggatgggtc agtgcacgcc tagaacaggc
     5821 tttggggact attagttttg tgcccaatat tgaaacggcg ggagttgtcg tcagcggaac
     5881 ggggctgccg gctacagctc agttgtttta ccgccgggca ggggatgtaa actggcagcc
     5941 agggcatgcc cttttgcgca tcaaagacgg acgtttgatt ggtagcttgt ttaacttggc
     6001 tgcatctacc tcttatgaag tgcgcgtttc tgatggcgta acagaagttt ctggttcgtt
     6061 taccactcaa gccgatgagt tactttttgc gcccacgaat attatttatg taaatgcatc
     6121 tgcacaggca ggtggggatg ggtctgcctc tgcgccattt cgtacgatac aggaaggggt
     6181 caatcgtgca gcaccaggca cgcaagtttt ggtggcagat gggatttaca gtgagtcggt
     6241 cacattcccg gcttcgggaa cggctggaag ctggattcag gtcaaggcac agggtggagg
     6301 cgcgatttta gatgggtccg cttcggtttt agcaacggat tggaatcctg ttgaaggcaa
     6361 agccaatgtc tggtttacga agattagccc ggggattaag tatctggcgc gggatggatt
     6421 gcgtttttat caatatgatt ttttgagtga cctttacgac caacgcggac ataacaatgt
     6481 gccgatgagt gaaggttggt tttatgaccc tatcaatggt cgtttgtatg tgcgcatcaa
     6541 tcgtgagccg cataaatata catggcaagt tccaaagttc aatgccgcgt ttatgataga
     6601 tggacgtgat tggatttgga tcgaaggctt tgagatgcgc tactatggca cagaatttgg
     6661 ctgtggcgtt tgttctaaaa attcttcgca tgttgtgatt cgcaagaaca aaattcatca
     6721 tattcaaaac ccggtctttg tagaatggac aggcggagaa gaccgcggca atgatactcg
     6781 tatcgaatac aacgaaatgt acgacacact taccggtggc gattgggcat ggggcgctat
     6841 taagagtacg gcaatggagt ctatggcaat tgtcttgcga gggcatacgg gagccattgt
     6901 acgtggcaat accatccacc attatttcaa tggcatttac accagctcct cggctgcatt
     6961 agacaaccct ggtgttgcct ttgatgcaga tatttacaac aactatgtct atcatatcgg
     7021 ggatgatgcg tttgagcctg aagggacagc cgttaatcat cgttttcgaa ataacaaagt
     7081 ggattcgact ctagttggta tttcgcttgc ccctgtaacc atgggtccag tttggattgt
     7141 acggaacacc ttcacgaact ttacggctcg cagtgtgaag tgggatcgcg gctcagatgg
     7201 ttgggtactg atgtatcaca acactagttt tacgacttat accaataccc attcagttga
     7261 gctgattagc gctgtgaaaa actcaatctt gcggaataac attttccatg caaacggtta
     7321 ttctgtagag gcgcacaacc ccggttcaac gggtcatgat tggaattaca acgattggtt
     7381 tgcattgaat tcaaatgctc cttttatgtg ggaaggcatt tcatatgcca ctttgaatca
     7441 gtggtgccgt tcaacagggt tagattgcaa tggctatgaa gattcaccgg gcttatccac
     7501 agatatgact ttgttatcaa acagccccaa cattgaccgt ggcgttctga ttcctggaat
     7561 taacgatcaa tttatgggca gtgcgcctga tcttggtgca tatgagtctg tgtatggaac
     7621 atctcctgct acacccgttg cgaccaacac acctttgcca ctgccaactg atactccaac
     7681 cccgatgcct gtttattcat cgccggtggt tacttccatt actttagcag acccaacacc
     7741 aaataatgca gagcttgtac gcttttttgt gaacttttca gagccggtca acaatgtaga
     7801 tgtcaatgat tttatgatga ccgcaacagg ttccatttca aattacacca tccttgatgc
     7861 gtaccctgtc ggaagcgcgt atgcgattac agtgtatacc ggtgtagggg atggaaccct
     7921 gcgtcttgat cttgtggata acgacagcat cttgagcgca acgtctatgc ctttgggtgg
     7981 agttgggatt ggtaacggga acttctcatt gggcaatgtc tatgtgatta acaaaaacgc
     8041 cccattcgtg acctccatcc tgcgtgctga cccaagccct tccaacgctg gaagtgtgcg
     8101 cttcagcatt acattctccg aatccgttag cggtgtggat gtggctgatt ttgtgctgtc
     8161 cacttcgggt ttgagtgagg cgggcattgc atccataagc ggttcaggca ctgcctatat
     8221 ggtgactgtc aacacaggta ttggaagtgg aaccctccgg cttgacttga atgacgatga
     8281 tagtgttgtt gattcggctg gcgccccagt gggaggaatt ggagtgggga atgggaactt
     8341 cgctgctggc gagacctata cgattgaccg caatatcccg ttgattgaaa ctgtttcttt
     8401 ctcttcagac ggagcccaag acggttgggt gatggagtcg aaagagacca gcaataaagg
     8461 tggaaccttg aactctaatg cgatcacctt ccgtgtaggt gataatgcac aagataatca
     8521 atatcgttcc attctacagt tctctacagg ggcattgcct gataatgcca tcattacaca
     8581 aatgatattg acgatgcaac tagagactat tgttggaacg aatcccttta cgacccatcg
     8641 caatatgtgg gtggatattc gtcagggggc atttggtagt tttggccctt tgcagatcgg
     8701 tgcattgcag atttcagatt ttcaagcgcc tgccagtttg tataatgccg gaacaattca
     8761 gaacaatgcc gtgggagggt ggtattgggt taattttgac tcgggcgttt tctcacttgt
     8821 taatttgaaa ggggctactc agtttcgcct tggttttcaa ttggatgatg acgatgaccg
     8881 aaaagatgat tacctctcgt ttttcagcgg gaattactcg gttcaatcgg cgcgtccaca
     8941 gttgaccatt aagtattaca ttcccaagtg attttgatga gaaattctta ataaccttcc
     9001 agcataaaaa gctgaaaggt tttcccacct tgtaaaaggt ataattccgt gtagattttt
     9061 ttcttattta aggttgatta aatgactggt atttttattt catactcgcg aaaagactct
     9121 caagctgcca aaaagttgat ggagggcttt aagtccgtca aactggatgt gtgggtggac
     9181 tgggaggata ttccccctgc ggttggctgg ctggaccaga ttctgcatgg cattgagcag
     9241 gcggaagttt ttgtatttct catcagtcct gattcgatca aatcagaagt ctgtaaggtc
     9301 gaattggaac atgctcgtaa aaattcaaaa cgtatcatcc ctgtagtagc tcgtgatgtg
     9361 gttgtgcagg atgtgacccc catattgcga gacctcaact ggatttttat ccgtgagcaa
     9421 gatgattttg ctgctgggct tgaaaaagta aatctggcga tcaacctaga tattgaatgg
     9481 ttgcaggaac atagacgttt gcaagtccgc gccttggaat gggatcgtaa aaaagaatcc
     9541 agcttgttac tacgcggtac tgacctgcgt aatgcgagaa aaatgatcgt ggcgaatgag
     9601 aagaaagacc ctttcccttc gcctttgcaa aagctatata ttgaatccag ccggcgtggg
     9661 gaacaacttc gtcttacaac atacgtttct gccgcagtga ctttgtttat tatggtgttt
     9721 ctttcccttg tggcattgaa tcaaaggcaa gaagc
//
LOCUS       DAOQFT010000010         9749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3261_length_9749_cov_7.472354, whole genome shotgun sequence.
ACCESSION   DAOQFT010000010 DAOQFT010000000
VERSION     DAOQFT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3261_length_9749_cov_7.472354"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..264)
                     /locus_tag="PLR93_00450"
     CDS             complement(97..264)
                     /locus_tag="PLR93_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25453.1"
                     /translation="MPVAAAKDLPVTVLQQATPTPPVDSSEIGSTSGILVMGVVIVLI
                     IAVPLILQKRK"
     gene            731..1288
                     /locus_tag="PLR93_00455"
     CDS             731..1288
                     /locus_tag="PLR93_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNH25454.1"
                     /translation="MIKKFFDWLKQPLGLIAVGIVFLAVFSAAAYGLWTTQQAPAQPV
                     PGFPHKTHVAFGIQCLYCHPGAAKGPAAGIPSQAKCWACHNQIVKTRDSALLAPLKDA
                     VLSGTSLEWVPVAIVPDFVQFNHRAHIAAGKNCEECHGDMTQVTIAQNPQVMNMGWCL
                     NCHIQNAGEDQAKLIKLTDCGTCHY"
     gene            1310..>2133
                     /locus_tag="PLR93_00460"
     CDS             1310..>2133
                     /locus_tag="PLR93_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNH25455.1"
                     /translation="MSENFSRRDFLKLAGLGAATTAILTGCGPASRYVKREPYQQMPE
                     YNSVGQSTYYATTCRECAAGCGLVVRTYQGRALKTEGNANHPLNLGKTCARGQATLHG
                     LYNPDRVQGPSTGDWDSAIQTVADAFKTYKPSEIAFLMGMAPDHLFDLVSDLAKETGM
                     NAPVRYGALGMFEARATLSKAAENVLGQAGLPWFDIGKAQVVFSFGANFLETWISPLP
                     YTRGFVGLREGETKLRGTFVQFEARMSATGAKADHWVPVRPGTEGLVALAIGKLVS"
     assembly_gap    2134..2233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2234..3624
                     /locus_tag="PLR93_00465"
     CDS             <2234..3624
                     /locus_tag="PLR93_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNH25456.1"
                     /translation="RPGTEGLVALAIGKLVSELRGGATPRVFSGVKPAEVAEAAGVEL
                     AALEHLAEMFANSNALAIPGGPALGQSNGLEIAEAVLALNALSDNFGKPGGVYISALA
                     PNQTEYSRPASAEEMAAFIEQLKSGAIKALFVHGVNPVFELPASLGFKEALANVELIS
                     FATFPDETAAEAKYVLPDHHGLESWGYQRVATGVSLPVLSGAQPVVSPFYDTRATADV
                     LIAAAQLAGGKFAEALPYKDEVEFIQTKISSLLSDANGSFTATDPVTFMAYFQQYGGW
                     YKNADSLVAPDGSAVLDSNIKAAEPEFAGEGEFFFVPFVSPTLAEAGANKPWLQELPD
                     PTTTVMWNTWIEMNPETAHELGIENDDVVRVSSEAGEVEAYVYLYPAIRPDTIAMPFG
                     QGHTAYGRYAEKRGVNPADVLGAHFNGAGDLAFAGMKVKVEKVSSAVRGVGLSRMESR
                     MGVYGEALGEEH"
     gene            3627..4352
                     /locus_tag="PLR93_00470"
     CDS             3627..4352
                     /locus_tag="PLR93_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNH25457.1"
                     /translation="MIRPEEIKMSAEETGKWGMVINQDICTGCQACVAACAMENNVTF
                     VGEEDAGYGRSMHWIRIERFWEGEYPEVKMTPQQPMLCQQCGHATCEPVCPAFATVHS
                     QAEQLNLQVYNRCVGTRYCANNCPYQVRAFNWRDYQRPEPLPNQFNPDVTVRRMGVME
                     KCTFCIQRIHKAQDKAKAEGREVMDGEFTTACAQACPANAIVFGRVDQPESMVSQLAH
                     KGHGVKHLEELGTLPNVTYFKGG"
     gene            4355..5782
                     /gene="nrfD"
                     /locus_tag="PLR93_00475"
     CDS             4355..5782
                     /gene="nrfD"
                     /locus_tag="PLR93_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNH25458.1"
                     /translation="MSDNKHHAAHSHEEHLREKHLMLDPLPRGRMNEMVMESMLEKPT
                     WKFWVIFGFLAFVVVYFLFYKWAILITKGQGEAGVMRPDYWGIFLVNTVFWIGISHAG
                     TFISAILRVFRAEFRRPFTRAAELMTTFGLVQAGFSIFMHMGRVWLFYWLMPYPNERM
                     LWPNLHSPLSWDLLAITTYLLSSTMYLFLPLIPDVAMARDRSTGWRKNLYKILALGFR
                     GTEGEWTHLRNAMNIFAFAIIPVMFSVHTIVSWDFAAATRPGWSSTIFGPYFIVGALH
                     SGMGAAAVVLAVIRSTMKHMDYFVRGEHFDAIGKLMLIISMAWAYFFFNDYMVQWYGG
                     DKWTQQLLHFHEAGPLGWMWFGMLFCNVVVPWAILWNSKWRSTPWAITVVGLIINVGM
                     WFERYIIIPISVTINRMPFTWRMYTPGIEVPMGIGTVALFILLYMAASKLIPLIPVWE
                     VQEGQMAHELKKFGRETVVSVSELE"
     gene            5792..6334
                     /locus_tag="PLR93_00480"
     CDS             5792..6334
                     /locus_tag="PLR93_00480"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNH25459.1"
                     /translation="MTETKNTVVDILAVFSDLAPAADAIEQLRALGVSDAHMNVISGV
                     PVTEAMLGRPSQWTNVPRIALGGAILGFGAGAFLAFVAPYWYPYPIQVSTQAFVPGPP
                     TVVVLFELTMLGMLLSTFLGVFLDSYFPNYRPMKYVPEISDGKIAIYVECPDCDEKKI
                     TDLLKKLGAESVKPAEAQHL"
     gene            6331..6882
                     /locus_tag="PLR93_00485"
     CDS             6331..6882
                     /locus_tag="PLR93_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH25460.1"
                     /translation="MRLFKQLVAVFAVLAVLLAILMTFSYDIIKIQWVTFMAIQPSFD
                     DQEKPLAVPAQSIPVDGAAYLPGMGEPTNPVPADETSIARGKEIYQINCMMCHGETGQ
                     GNGTVAAFLVKKKPADLTSEPVQAKSDGSWFLTISNGIWNPNNTLFPERQFSGQMPPL
                     AENLSIRERWDVINYLRTLKASQ"
     gene            6895..7554
                     /locus_tag="PLR93_00490"
     CDS             6895..7554
                     /locus_tag="PLR93_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25461.1"
                     /translation="MKDDVRKYIYGAIAVFLVGVLAWVGIVFVSACGVSLSCNRGNTL
                     PDTTPVPTLIPATLPPMTVESNIVAPSDKCYVAGSDLIGAWVDAGSPEADVFEFTDAN
                     GATCEATFEDVQPLFTKANLWYSGSLSCISCHSVDLAVSPAQLDMSSYEGILAGSRRT
                     DDAPKGTDILGGGKWKSSLLYQFIVEAHADVPGHDAALSSDLMVYAGTPLPDVKATPT
                     P"
     gene            complement(7649..7738)
                     /locus_tag="PLR93_00495"
     tRNA            complement(7649..7738)
                     /locus_tag="PLR93_00495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7700..7702),aa:Ser,seq:gga)
     gene            complement(7787..9700)
                     /locus_tag="PLR93_00500"
     CDS             complement(7787..9700)
                     /locus_tag="PLR93_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25462.1"
                     /translation="MLIKINILDKINNLHPFLWGILKLSIAFFCFTFILFYPITILRP
                     ISLALRTGFGIIIPLTTIIIYIIFRIPGKLGEIMPLTAMMSLFALGLAGLWASGNTQS
                     VILNGLLPLTDAAGYYTDALRLQYGSNVSNFTAMRPLYAGLLSFFLWISNNNLMIAVS
                     IFTAITGFSCYLACKEIQKTHGAEVAVFFLILTFLYYRHHSGTTMSESLGVPVSLLGV
                     ALLWKGIYSKNEGTTLFGIGMIALALNIRPGAMFVLPGLLLWGGWIFRKEKKFSFKFF
                     ISGLTVVLSVFYINSRMIDFVAGPSGVAFENFSWAFYGLSSGGKSWTYIFEAHPELRL
                     LQDSEVTPTIYKLAFKQILNNPSLMIKGSFFYWRMFFSDSWYNAYAFVAGENYWISEI
                     ARWGMYILGALGIYKLLKNYQDPYTSLAGITAVGVLASVPFVPPTDAYRVRLYAATIP
                     FFILLPGMGVSLLLDAVPQRLLSPKFTFLNKNSTSAIFSIVLILTLLLSPIYIKTAGT
                     SPTFSQTSCPASLDSFLIRYDDGSSFTVYRESQVFIDQMPDFHMSTFRRNAHSLPDMN
                     LITTLENIEPPKTITYSIDLLTNNQAIIIAEPELLPVSGVYLLLCGKFIYEPNFTPYS
                     FFYVEQVFSVEGH"
BASE COUNT         2405 a         2252 c         2366 g         2626 t
ORIGIN      
        1 atcaatatct tttcaggcat gagtggcact ataccataaa ggaatccctg atctgttaag
       61 gtgaggttaa tggcttgaac ttcaagccaa tccgttttat tttctttttt ggaggatcaa
      121 cggcacagcg atgatcagaa cgatgaccac acccatgacc aggattccgc ttgtggagcc
      181 gatctctgaa ctgtcaacgg gaggggtagg ggtggcttgc tgtaaaacgg tgaccggcaa
      241 atctttggcg gcagctacag gcatgttaaa gtttgtgatc accaacgtga gagttaggat
      301 ggctatcaaa aaaatgcacg cgttgaaaat attttttgtt ttcatcgtag gttcattata
      361 aggtgaaacc attcttttgt tcggagtgtt cttttaaaac ataacatcaa aatcacatca
      421 atattaaagt gcgttaagtt gagaaatttt gaccaaaaaa gtcctgtttg ggcttaaaac
      481 acaaatcgga taacttgtac ctttttgcac gttcgatgta taatttacaa gttgtgcatc
      541 agtgcaggca gatgcacgaa aaataccctc agagcgcggc tgtcttgtaa agtaatttgg
      601 gtgggttctt tttataaaga gagagaccag caaaagcgct acatgtttgt cattaagaag
      661 ttggtaattt tgcaaacgcc accattggca acaatggcgg cgttacgctt agtttatttc
      721 aggagcatcc atgatcaaaa agtttttcga ttggctgaaa cagcctcttg gtctgatcgc
      781 ggtggggatc gtgttcctgg cagtgtttag cgcggctgct tatgggttat ggactaccca
      841 acaagcacct gctcaacctg ttccagggtt ccctcataag acccatgttg ccttcggtat
      901 tcaatgtttg tattgccatc cgggcgcggc gaagggtcct gcggcgggca ttccatcaca
      961 agcaaagtgc tgggcatgcc acaatcagat cgttaagacc cgtgatagtg ctttgcttgc
     1021 cccacttaag gacgcagttc tgagtggcac atcgcttgaa tgggtccccg ttgccatcgt
     1081 gccagacttt gtacagttca accatcgtgc gcatattgcg gcagggaaga actgtgaaga
     1141 atgtcacggc gatatgactc aagtaaccat tgcccaaaat ccacaagtga tgaacatggg
     1201 ctggtgtttg aactgtcaca tccagaatgc cggtgaagat caagccaaat tgatcaaact
     1261 gacagactgc ggaacgtgtc actattaaac cgggtgaaag gaaacctata tgagcgaaaa
     1321 tttttcccgg cgtgattttc taaaactggc tggtttagga gcggcgacca ctgcgatcct
     1381 cacaggttgt ggtcccgctt cgcgttatgt taagcgcgaa ccttatcagc agatgcctga
     1441 atacaattcg gtcgggcaga gtacctatta cgccactaca tgccgcgaat gcgcggcggg
     1501 atgtggtctc gttgtgcgca cctatcaggg tcgtgctctc aagaccgaag gcaatgccaa
     1561 tcatcccctc aatctcggta agacctgtgc acgcgggcaa gccactcttc acggcttata
     1621 caacccagac cgtgttcaag gtccttccac cggagattgg gactctgcta ttcaaaccgt
     1681 agccgatgcc ttcaaaacct acaaacccag tgagatcgct ttcctcatgg gtatggcacc
     1741 agaccacctc ttcgaccttg tctctgacct tgcaaaagaa accggcatga atgctcctgt
     1801 ccgctatggt gcacttggca tgttcgaagc tcgcgccact cttagcaaag ccgccgaaaa
     1861 tgttttgggt caggctggct taccgtggtt cgatattggc aaggctcagg tggttttctc
     1921 ctttggtgca aacttcctcg aaacttggat ctctccgctc ccttacacac gtggctttgt
     1981 cggcttgcgt gaaggcgaga caaaactgcg cggcaccttc gttcaatttg aagcgaggat
     2041 gtctgctact ggtgccaagg ctgaccattg ggtgccggtt cgccccggga ccgaaggctt
     2101 ggttgccctt gcaatcggta aactcgtctc agannnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnttcgccc cgggaccgaa ggcttggttg cccttgcaat cggtaaactc
     2281 gtctcagagc ttcgcggcgg cgcaacacca cgcgtcttct ccggcgtgaa accggctgaa
     2341 gtcgctgaag cagctggcgt cgagttggcg gcgttggaac acctcgctga aatgtttgcc
     2401 aattcaaacg cgcttgccat tcctggcggt cctgcccttg gtcaatccaa cggtttggaa
     2461 attgccgaag cggtgctggc gttgaatgct ctctccgata acttcggcaa acctggtggc
     2521 gtgtacattt ctgctctcgc cccgaaccag accgaatata gccgccctgc ttctgccgaa
     2581 gaaatggctg cttttatcga acagttgaag agcggcgcga tcaaagcctt gtttgttcac
     2641 ggtgtgaacc cggtctttga attgcctgct tctctcggct tcaaagaagc gcttgcgaat
     2701 gtagaactta tctcgtttgc aaccttcccc gatgaaaccg ccgctgaggc aaaatatgtg
     2761 ttgcccgacc accacggact tgagtcttgg ggttaccagc gtgtagccac tggcgtgtct
     2821 ttgccggtgc tctctggcgc acagcctgtt gtttctccct tctacgatac tcgcgccacg
     2881 gctgatgtgt taattgctgc ggctcaattg gctggcggca agtttgccga ggcccttcca
     2941 tataaagatg aagttgagtt cattcagacc aagatcagct ctttgctgag tgatgcgaat
     3001 ggctccttca ctgctacaga ccctgtaacc ttcatggcgt acttccagca gtatggcggt
     3061 tggtataaga acgccgactc tttggtagcg ccagatggct ctgctgtgct tgatagcaat
     3121 atcaaagccg ctgagcctga atttgctggc gaaggtgaat tctttttcgt gccgttcgtc
     3181 tctccgactc tcgcagaggc gggcgcgaac aaaccttggt tacaggaact ccctgacccc
     3241 actacaacgg tcatgtggaa tacctggatc gaaatgaatc ctgaaaccgc tcacgaactt
     3301 ggcattgaaa atgacgatgt ggttcgcgtc agcagcgaag ccggtgaagt ggaagcgtat
     3361 gtgtacttgt atccggcgat ccgccccgat acgattgcaa tgccgttcgg tcagggtcat
     3421 acggcttatg ggcgttatgc tgagaagcgt ggggtcaacc ccgctgatgt gcttggcgct
     3481 cactttaacg gagccggtga tcttgctttt gctggtatga aagtcaaagt tgaaaaggtc
     3541 tccagcgctg tacgtggtgt tggtctttcc cgcatggaaa gccgcatggg cgtgtacggc
     3601 gaggcgctgg gtgaggagca ttaatcatga ttcgacccga agaaatcaaa atgagcgccg
     3661 aagaaaccgg caaatgggga atggttatca accaagatat ttgtaccggc tgccaggctt
     3721 gtgtggcggc ttgtgcaatg gaaaataatg tcactttcgt cggtgaagaa gatgctgggt
     3781 atgggcgttc catgcactgg atccgcattg aacgtttttg ggagggtgaa tatcctgagg
     3841 tcaagatgac cccgcaacag ccaatgctgt gccagcagtg cggtcatgcc acttgtgaac
     3901 cggtctgccc ggcgtttgcc acggtccatt ctcaggctga gcagttgaac ttgcaggttt
     3961 acaaccgctg tgtgggtacc cgttactgcg caaacaactg cccttaccag gttcgtgctt
     4021 tcaactggcg tgattatcag cgccctgaac cgcttcctaa tcagttcaac cctgatgtga
     4081 ctgttcgtcg tatgggtgtg atggagaagt gtactttctg tatccagcgc attcacaagg
     4141 cgcaagataa agccaaagcc gaaggacgcg aagtaatgga tggtgaattc accaccgctt
     4201 gcgcccaagc ctgccctgcc aacgccattg tcttcggacg tgtggaccag cctgaatcga
     4261 tggtctctca attggctcac aaaggacatg gcgtcaaaca tcttgaagaa cttggcacgc
     4321 ttccaaacgt gacctacttc aagggagggt aattatgtca gataataaac atcacgcagc
     4381 gcattcgcac gaagaacacc tgcgtgaaaa acacttgatg ctcgacccgc ttcctcgcgg
     4441 tcgcatgaac gaaatggtca tggagtccat gctggaaaaa cccacatgga aattctgggt
     4501 gatctttggt ttcttggcat ttgtagtggt gtacttcctc ttctataagt gggcgatcct
     4561 cattacaaaa ggtcagggtg aagccggcgt gatgcgccca gactattggg gtatcttctt
     4621 ggttaatacc gtgttttgga tcggtatcag tcatgcaggt acattcatct ccgcgatttt
     4681 gcgcgtgttc cgggcagaat ttcgccgtcc gtttacccgt gcggcagaat tgatgaccac
     4741 cttcggtttg gtgcaagctg gcttctcgat cttcatgcat atgggtcgtg tttggctttt
     4801 ctactggctg atgccttacc ccaatgaacg catgttgtgg cccaaccttc actctccact
     4861 ttcatgggac ttgttggcga tcacaactta cttgctttct tccaccatgt atctcttcct
     4921 gccgctcatc cctgatgtag cgatggctcg tgaccgctcc actggttggc gcaagaattt
     4981 gtataagatc cttgctctcg gcttccgcgg tacagaaggg gagtggaccc acctccgcaa
     5041 cgcgatgaac attttcgcct ttgcgatcat cccggtcatg ttctctgttc ataccatcgt
     5101 gtcttgggac tttgctgcgg cgacccgccc tggttggtct tccaccatct ttggtccgta
     5161 ctttatcgtg ggtgctttgc attccggtat gggtgcggct gcggttgtat tggctgtaat
     5221 tcgcagcacc atgaaacaca tggattactt tgtgcgcggt gaacatttcg atgcgattgg
     5281 taagctcatg ctgattattt ccatggcttg ggcatacttc ttcttcaacg attacatggt
     5341 acagtggtat ggtggcgata agtggacaca gcagttactc catttccatg aagcgggtcc
     5401 gctcgggtgg atgtggtttg gtatgttgtt ctgtaacgtt gtagtgcctt gggcgatcct
     5461 ttggaattcc aaatggcgat ccacaccatg ggcgatcacc gttgtcggtt tgatcatcaa
     5521 cgttggtatg tggttcgaac gctacatcat tatcccgatc tctgtcacca tcaaccgtat
     5581 gccgttcact tggcgtatgt acacacctgg tatcgaagtc cccatgggta tcggtactgt
     5641 tgccctcttc atcctgcttt atatggcggc ttccaagttg attccgctta tccctgtttg
     5701 ggaagtgcag gaaggtcaga tggcacacga actcaagaaa tttggacgtg agaccgtggt
     5761 ctctgtcagc gaacttgaat aggaggatac gatgactgaa acgaaaaata ctgttgtcga
     5821 tatactcgcg gtcttttcgg acctcgcgcc tgctgcggac gccattgaac aactccgcgc
     5881 cttgggcgtt agtgatgctc acatgaatgt catctctggt gttccagtca ctgaagctat
     5941 gttgggtcgc cccagccaat ggacgaatgt gccgcgcatt gctttaggtg gcgcaattct
     6001 cggttttggt gcaggtgcct tcctcgcttt tgtggcgccc tattggtatc cgtacccgat
     6061 ccaagttagc actcaagctt ttgtaccggg tcctcctact gtggttgttc tctttgaatt
     6121 aaccatgctt ggtatgcttc tttccacctt ccttggtgtg ttccttgata gctacttccc
     6181 gaactatcgt ccgatgaagt atgtgccaga gatcagtgat ggcaagatcg ctatctatgt
     6241 agagtgccca gactgcgatg agaagaaaat cacagacctg ctaaagaaac ttggcgctga
     6301 atctgtgaag cctgcggagg cacaacacct atgagacttt tcaaacaact tgttgctgta
     6361 tttgctgttc tcgccgtcct ccttgccatc ttgatgacgt tctcatatga catcattaag
     6421 atccagtggg tgacatttat ggcgattcaa ccctcttttg atgatcagga aaaacctttg
     6481 gcagttcctg cacagtccat tccggtggac ggcgcggcgt atttgcctgg catgggggag
     6541 cccaccaatc cggttcctgc agatgaaacc tctatcgccc gtggaaaaga gatctatcag
     6601 atcaactgca tgatgtgcca tggcgaaact gggcagggga acggaaccgt ggcagcattc
     6661 ctcgtaaaga agaagcctgc tgacctcact agtgaacctg ttcaagctaa atcggatggt
     6721 agctggttcc tgacgatctc taacggtatt tggaatccca acaacactct tttcccagaa
     6781 cgacagttct ccgggcaaat gccacctttg gctgaaaatc tcagcattcg cgagcgttgg
     6841 gatgtgatca actacctccg cacgctaaaa gccagtcagt aggagatgca tgcaatgaaa
     6901 gacgatgttc gaaaatatat ttacggtgca atcgccgtat ttctggtagg cgtgctggct
     6961 tgggtaggga tcgtgtttgt gagtgcttgt ggtgtttctc tttcctgcaa ccgcggcaat
     7021 actttgcctg ataccacccc tgttccaacc ttgattccag ccacacttcc gcctatgact
     7081 gtggaatcca acatagtggc accctctgat aaatgctatg ttgctggctc cgatttgatc
     7141 ggcgcttggg tggatgcagg ctctccagaa gcagatgttt ttgagttcac agatgccaat
     7201 ggtgcaacct gtgaagccac ttttgaggat gtgcagcctt tattcactaa agctaacctt
     7261 tggtattccg gctcgctctc ctgcatttcc tgtcactctg ttgatttggc tgtttctccc
     7321 gcccaattgg acatgagcag ctatgaaggc atccttgctg ggtcacgccg tacggatgat
     7381 gcacctaagg gtaccgatat ccttggcggt ggaaagtgga aatcgtccct gttatatcag
     7441 ttcattgtag aggcacatgc cgatgtccct gggcatgatg cggctctatc ctctgacctg
     7501 atggtatatg caggaactcc gctccctgat gtgaaagcta caccaacacc gtagtatccg
     7561 gaataaaaaa aggacctgat ttcatgatca ggtccttttt tgttaggaaa atttgataaa
     7621 aagaaagccc ctcattgagg ggcatggctg gcggagaggg cgggattcga acccgcgaac
     7681 ctttgtgggt tacacgcttt ccaagcgtgc gcgctaagcc agactacgcg acctctccaa
     7741 actaacagcg ggcagtatta taccatgcgt ttctatattg gcaagtttaa tgcccttcaa
     7801 ctgaaaagac ttgctcgacg taaaagaagg agtatggagt gaaattaggt tcataaataa
     7861 atttgccgca aagaagcagg tatacacctg aaacaggcaa taattcaggt tcggcaataa
     7921 taatagcctg gttgttcgtt aacaagtcga tggagtaagt gattgtttta ggcggttcta
     7981 tattttctaa ggttgttatg agattcatat ctggcagact gtgagcattc cgacgaaaag
     8041 tactcatatg aaaatctggc atttgatcaa taaacacttg gctctctcta tagacagtga
     8101 aagagctacc atcatcatac cttatgagaa aactatctaa actagccggg caacttgttt
     8161 gtgaaaatgt aggcgaggtt cctgctgttt ttatgtatat tggtgataga agaagtgtaa
     8221 gtattaatac aattgagaag atagcacttg ttgagttttt attaagaaaa gtaaatttag
     8281 ggcttaagag tcgttgagga actgcatcga gcaatagaga gactcccatt cctggtaata
     8341 gaatgaaaaa aggaattgtc gccgcgtaaa gccgaactct atatgcatct gtcggtggga
     8401 cgaagggtac tgaggctagt actcctactg ctgtaattcc agctagactt gtatacggat
     8461 cttgatagtt ttttagtaat ttataaatcc ctaatgctcc aagaatatac atgccccatc
     8521 gtgctatttc actgatccaa tagttttcac cagctacaaa agcataggcg ttgtaccatg
     8581 aatcagagaa aaacattcgc cagtaaaaaa atgaaccctt aatcataaga gatggattgt
     8641 tcagtatttg tttaaaagcg agtttataaa tggtaggtgt tacttctgag tcttgtagga
     8701 gacgtaattc agggtgtgct tcaaaaatat aagtccaaga cttgccgccg gaagataagc
     8761 cataaaatgc ccacgagaag ttttcaaatg ctactccact tggaccagca acaaaatcta
     8821 tcatgcgact atttatataa aacacagata aaactacagt tagtccagag ataaagaatt
     8881 tgaaagaaaa ctttttttct tttcgaaata tccaacctcc ccatagtaaa agtccgggta
     8941 gaacaaacat agctcctggg cgaatattca gggctaaagc aatcatccct attccaaata
     9001 aagtagttcc ttcatttttt gaataaatcc ccttccaaag taaagctaca ccaagcaagc
     9061 ttactggaac tcctaaactt tccgacatgg tagttccgct gtggtggcga taatatagaa
     9121 aggttagaat taagaaaaat acagcaactt cagccccatg tgttttttgg atctctttac
     9181 aagctaagta acaagaaaaa cctgtaattg ccgtgaaaat tgatacagca atcattaggt
     9241 tgttattcga tatccaaaga aaaaaagata ataatcctgc atacagcgga cgcatcgcag
     9301 taaagttgga aacatttgat ccatattgca atcgtagtgc atcagtataa taaccagcag
     9361 catctgtaag cggaagcagt ccatttaaaa ttacagactg agtgttccct gatgcccata
     9421 atccagctaa tcccaatgca aacagtgaca tcattgctgt aagaggcatg atttctccaa
     9481 gtttgccagg aattcgaaaa ataatgtaaa ttattattgt cgtaagaggg atgataatgc
     9541 cgaatcctgt tcgtagagca aggctaatag ggcgcaggat tgttatggga tagaaaagaa
     9601 taaaagtaaa gcaaaaaaat gctatgctta acttcagaat tccccagagg aaagggtgaa
     9661 gattatttat tttgtctaaa atattgattt ttattaacat agaatagtta ttataaatga
     9721 attatctatg ccttaaatga gttacaggt
//
LOCUS       DAOQFT010000011         9661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3306_length_9661_cov_7.992921, whole genome shotgun sequence.
ACCESSION   DAOQFT010000011 DAOQFT010000000
VERSION     DAOQFT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3306_length_9661_cov_7.992921"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="PLR93_00505"
     CDS             complement(<1..1639)
                     /locus_tag="PLR93_00505"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNH25463.1"
                     /translation="MPIHTSLNYWILETRHSAYAFGLNKANLLSHCYWGKRLPFVNDY
                     PSPFEPSGWASTDGYDHFIPEEYPGYAGTKYTDPTLKVTFADGVRDVVLSFVDFKTSA
                     SPQPELALRFKDSAYPLQLTLHYRVHEEYDLIERWVTLENLGSESISIERAFSAKWTF
                     PHGNGYSLTHMYGRHIDEFNMQRETLTPGLKVIESRRMIPSHRAVPWFTVEKNASEDH
                     GDVFFGTLAWSGNFKITAEVTEYASTRLSIGLNDWDFAWQLKPNEPLVTPSAISGYTP
                     NGFGDASRIFHDYVRNELLPHGKVLHKILYNSWEATLFNVDEDSQSHLAEIAANMGVE
                     LFVMDDGWFHNRNWDNAGLGDWFPDQKKFPNGLGGLIKSVNDLGMDFGLWVEPEMVNP
                     DSDLYRAHPDWVIHFPTRVRTEARNQLMLNMARSDVQDYIITTLDKILSENNIAFIKW
                     DMNRPASEPGWNDAPGDPRELWVRYVYGLYRVGSELRRRHPKVIWQSCASGGGRPDFG
                     ILQFADQVWVSDNTEATKRLSIQEGFSQFMPAITMEAW"
     gene            complement(1698..2708)
                     /locus_tag="PLR93_00510"
     CDS             complement(1698..2708)
                     /locus_tag="PLR93_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH25464.1"
                     /translation="MANKKKSPTIYDVAELSGVSISTISRVLNSPDKVNTETRQQVMS
                     AIDRLGFVPKAEARARAMQNTNRIGVITPFFTAPSFVQRLRGVASRLSRENYELVIYP
                     VDSEEQLQGYLSSIPVMRNLDGLIIMSLAVESSDVERLISNKMETVLIEFSNDQLNSI
                     VIDDNQGGRIVAEHLIQKGYKTFGFLGDIEPPERNIIHPVRSRLEGFQSVLKEAGLQL
                     PAKNIKQAFYAHESSREAAHELLTMPDRPSAIFAASDNQALSIMKVARQLNMRIPDDV
                     AVVGFDDIDMADYVDLTTVRQHLDESGRLSAEMVLGRIADPSRATQHIYLPLNLIERQ
                     TT"
     gene            2882..>3858
                     /locus_tag="PLR93_00515"
     CDS             2882..>3858
                     /locus_tag="PLR93_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH25465.1"
                     /translation="MRTKKRAVTIQDVAKAAGVSVSTVSRVLNGKTDVAGETQERILK
                     IINKLGFTSNLAARSMRSLKKNLIGLVVPDIHPYSVEIMKGVNKAIAESNYDLLVYTT
                     GDALKHGATLHEQHYISLLSNSITDGVIIVASATAEFTAEAPIVAVDPHVPNPKYPSV
                     QGMNYHGALDAMEYLFKLGHQRIGFMSGREGNVSGDRRFQGYKNALKNAGIEFDSSLV
                     VLGDFVQKTAYKNALKLLQIEDRPTAIFAANDQSAIGVYQAAEELGMNIPADLSVIGF
                     DNISEAKYLGLTSVDQFLADMGYMAVQMLIKLIEGEPLEFDVHKMQTKLV"
     assembly_gap    3859..3958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4107..5570
                     /locus_tag="PLR93_00520"
     CDS             4107..5570
                     /locus_tag="PLR93_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH25466.1"
                     /translation="MKRLSYILSLLVVVSMFLAACAPAATEEAPAATEAAAEATDAPA
                     ATEAAPAELSGTLNVWSFTNEIRTMAIAFEGVHPNVDVVYTMIPMTNGEYQTKVKAAA
                     GTADSPDVVALEAAFVKEWVESDLLADQSDLLPLAEELKTFPSVVEVGTYDGITKAYS
                     YQATPGAFFYRRSIAKECLGTDDPDAVQAMVADLDKFVETAAKIKECGTGDYFTVGTS
                     GEMFNPFLANREQPWVVDGELVIDPMVLEYVKFAKLMRENGYESQATQWSEGWFAGMN
                     DTLKDANGTPKKVFSYFLPTWGLPYVLIPNSKSDTTDTGGDWAMINGPMAYQWGGTWV
                     GALEGSPNAELAKEFIKFVALNEDNLTNWATGVYTNEYLKAIDPETPDDQAQAPGDFV
                     SSQVVVEKITSTFDGSDLAKWLGGQNSYAAFAKAAPDVNGALLTGSDDAIQRALNDPL
                     ASYLNGDITEAEMYTQWLDAVRNEFPDLVIPEPPVTE"
     gene            5662..6600
                     /locus_tag="PLR93_00525"
     CDS             5662..6600
                     /locus_tag="PLR93_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNH25467.1"
                     /translation="MGLIKLKKHQYGYIFIAPFIIGFLAFGLYPVYNTLALSFTDATL
                     MTKSAHFVGFKNFERLFADDVFLGAVKNTWLLWALNFIPQVGIAMLLSVWFTDARLKI
                     KGVGVWRAVFFLPNLLVPASVAALFFAFFSFYGPVNQFMVRFGFLPEAMHYLQSVPVT
                     RGLVIFIQWWMWFGQTVIIVMAGMTSIPVSLYEAAMVDGATTSQMFRRITLPLLKPIL
                     IYIFVTSLVGGMQMFDIPFLLTDGRGSPNSSIQTNNILMYMKFASSKGHIGAASSVGV
                     LVFLMTSICALGIFYFLRERDTVSEKPKKSLFGKRS"
     gene            6584..7459
                     /locus_tag="PLR93_00530"
     CDS             6584..7459
                     /locus_tag="PLR93_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNH25468.1"
                     /translation="MENVRESDKAMLNNYKFTTTVLRILVYFMLFALLVVTIVPIWLL
                     IVNATRSTTEIQQGLSIMPSTHLIENYNILLGKGLNLPRGFANSTFLAVSSTVVSVYF
                     SMLTAYGIVVYDFKGKHAFSNFIVVLVMIPMQLSIIGFYQYMSKLGLTDNYASLILPL
                     IANAGGVFFGKQYLESMIIQDLIDAARIDGASEMAIFHQVMMPLAIPGAVTMGIFAFI
                     TSWNNFFNAFILISSIEKYTLPMLVQTLRGDVYRTEFGAIYLGLAATIVPIILLYIFF
                     SRYVVSGIAIGAVKE"
     gene            7626..8792
                     /gene="xylA"
                     /locus_tag="PLR93_00535"
     CDS             7626..8792
                     /gene="xylA"
                     /locus_tag="PLR93_00535"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNH25469.1"
                     /translation="MSKYSPSPENKFTFGLWTVGNVGRDPFGSPVRDGKSPADLVRLL
                     GEVGAYGVNFHDNDLIPIDATPSERDAILKEFKKALADTGIIVPMATTNLFSEPIFKD
                     GAFTSNDSKVRAYALQKTMNAIDLGVELGAKTYVFWGGREGTESDATKDPITSIKRSR
                     EAMNFLCEYVLDKKYDLKFALEAKPNEPRGDIYNSTTGHMLAFIETLDHPDMVGVNPE
                     VAHEHMAGLNFVHGVAQALEAGKLFHIDLNDQAFGRYDQDFRFGAVNIKSAFFLVKLL
                     EDNKYNNPRHFDAHAYRTEDYEGVKDFARGCMHTYLVLKEKAAQWNADKEIQSLVAEI
                     NADDGSMAQYFGKYSSAKASALKAQAFDRNAIASRGLKYERLDQLTIDVLLGVR"
     gene            8808..>9661
                     /locus_tag="PLR93_00540"
     CDS             8808..>9661
                     /locus_tag="PLR93_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNH25470.1"
                     /translation="MSNSNIAEKPIYLDTSRPREERVKDLISHLTLEEKVGLMSHPTN
                     GIPRLNIPAYNYWNEALHGVARNGRATVFPQAIAMAATWDKDLIHKIGNAISDEGRAK
                     YHAALKRNGYTQQYQGITFWSPNVNIFRDPRWGRGQETWGEDPFFTGEMAAEFVKGLQ
                     GNDPTYLKTAACAKHFAVHSGPEKDRHSFNAIVTKRELYDTYLPAFKKLVTEAKVEAV
                     MGAYNRTLDEVCCASKLLIEDILRGEWGFDGHFVSDCMALSDFHLNHKVTKDAAESAA
                     LALKYGCDL"
BASE COUNT         2387 a         2357 c         2341 g         2476 t
ORIGIN      
        1 cccaggcttc catggtgatg gcaggcatga attgcgaaaa gccttcttga atggacagac
       61 gcttggtcgc ttcggtgtta tcgctgaccc aaacctgatc tgcaaattgc aggattccaa
      121 aatcgggtcg tccgccgccg ctggcgcaag attgccaaat gaccttcggg tgccgccgac
      181 gcagttcact ccccacgcga tacaagccat acacataacg aacccataac tcacgcgggt
      241 cacctggcgc atcattccag ccgggttcac tggcaggacg gttcatgtcc catttgatga
      301 aggcgatgtt gttctcgctg agaattttat cgagcgtggt gatgatgtaa tcctgcacat
      361 cacttcgcgc catgttgagc atgagttgat tgcgggcttc tgtgcgcaca cgggttggga
      421 agtgaatgac ccaatcggga tgagcgcggt acagatcgct atcggggttg accatctctg
      481 gttcgaccca caagccaaag tccatgccga ggtcgttgac gcttttaatc aagccgccga
      541 gtccgttcgg gaattttttc tgatcgggga accagtcgcc cagaccggca ttgtcccagt
      601 tgcggttgtg gaaccagcca tcgtccatga cgaagagttc cacgcccatg ttcgcggcaa
      661 tttcggcgag atgtgattgc gagtcttcgt ccacgttgaa gagagtcgct tcccatgagt
      721 tgtagagaat tttgtgcagc acttttccat gcggcaggag ttcattgcgc acataatcat
      781 ggaagatgcg gctggcgtcg ccgaatccgt ttggggtgta gccggaaatc gcagaaggag
      841 tcaccagcgg ttcattcggc ttgagctgcc aagcgaagtc ccaatcgttg agcccgatgc
      901 tcaaacgggt cgaagcatac tcggtcactt cggcggtgat cttgaaattt ccgctccagg
      961 cgagcgtgcc gaagaacacg tcaccgtgat cttcgctggc gttcttctcc accgtgaacc
     1021 acggcacagc gcgatgactt ggaatcatgc gtcggctttc gatgactttc aaaccgggag
     1081 tgagcgtttc gcgctgcatg ttgaactcgt cgatgtgacg cccgtacatg tgcgtgagtg
     1141 agtaaccgtt cccgtgcggg aaggtccact tggcggagaa ggcgcgctcg atggaaatag
     1201 attcactacc aaggttttcg agcgtgaccc agcgttcgat caggtcgtat tcttcatgga
     1261 cacggtaatg gagagtcaac tgcagcgggt aggctgagtc tttgaagcgc agagcaagct
     1321 ccggctgggg tgaggcgctg gttttaaaat ccacaaaaga gagaaccaca tcgcgcacac
     1381 catcggcgaa ggtcactttg agagtcggat cggtatattt ggtgcctgca taaccgggat
     1441 attcttcggg gatgaaatga tcgtatccat cggtggacgc ccagcccgac ggctcaaatg
     1501 gactggggta atcgttgacg aaaggcagac gtttgcccca ataacaatgt gagagcaggt
     1561 tggctttgtt gagcccaaag gcataggcgc tgtgacgtgt ttcaaggatc cagtagttga
     1621 gggatgtgtg aataggcatg agtcctctat tgaaattcaa aaagatgaat gcagaataat
     1681 tccacattca tcttttgtta tgtcgtttgt cgttcgatca ggttaagggg aaggtaaatg
     1741 tgctgcgtgg cacgcgaagg gtcggcgatg cgcccgagca ccatttcagc cgagagcctg
     1801 cccgattcat ccaaatgctg gcggacggtg gtgaggtcga cataatctgc catgtcaatg
     1861 tcatcgaagc caactacggc aacatcgtca gggatacgca tgtttaactg ccgtgccact
     1921 ttcataatgc tcaatgcttg gttatcagag gcggcaaaga tggcagaggg gcggtctggc
     1981 atggtgagca attcatgcgc ggcttcacgt gaactttcat gtgcataaaa tgcctgcttg
     2041 atgtttttcg cagggagttg caagcccgcc tctttcaaca ccgattgaaa cccttccaag
     2101 cgggaacgca cagggtggat gatattgcgt tcaggtggtt cgatatcccc tagaaagcca
     2161 aacgttttat agcctttttg aatcagatgc tctgcaacaa tgcgcccacc ctgattgtcg
     2221 tcgatcacga tgctgttcaa ctggtcgttt gagaactcga tcagcaccgt ttccattttg
     2281 ttgctgatca ggcgctccac atcactgctc tctacagcca acgacatgat gatcagccca
     2341 tctaaatttc gcataacagg aatagaagag agatagccct gcaattgctc ttccgaatcg
     2401 accgggtaaa tgaccagctc ataattctcg cgcgaaagtc tgcttgccac gccgcgcaag
     2461 cgttggacga aggatggggc ggtaaaaaat ggggtgatga ccccgatgcg gttggtgttc
     2521 tgcatggcac gcgcacgcgc ctcagccttc gggacgaagc ccagccggtc aatggcagac
     2581 ataacctgtt gacgggtttc ggtattaact ttatctggtg agtttagcac ccgcgaaatg
     2641 gtggaaatgc ttacgccaga aagttctgca acatcataaa tagtagggga ttttttctta
     2701 tttgccatat tgtatgtgaa agctaagtga aagcactttc agtttaacat gaaacagtct
     2761 caaattccag attcttagag tttcaattaa gtagttgact ttttattttt tttatgcaat
     2821 aataccggta acgtttccgg tattgatacc gaaaaataga gcattttcac caggaattgg
     2881 tatgcgaact aaaaaacgcg cagtgacaat tcaagatgtt gcaaaagcag caggtgtttc
     2941 tgtatcaacc gtttcgcgcg tattaaacgg taaaacggac gttgcggggg aaacacaaga
     3001 acgtattctt aagattatca ataaactcgg tttcacatcc aatctggcgg ctcgcagcat
     3061 gcgcagcctc aaaaagaatt tgatcgggtt ggtcgttcct gatattcacc cctactccgt
     3121 tgagatcatg aagggtgtta acaaggcgat cgcagagtca aattacgacc ttttggtata
     3181 caccacgggt gatgcgttga aacatggggc aaccttacac gagcaacact atatttctct
     3241 gcttagcaac tccattaccg atggggtaat cattgtggca tctgcgactg ccgaattcac
     3301 tgccgaagct cccattgtgg cagtggaccc gcatgtgccc aacccaaagt atccttcggt
     3361 tcaaggtatg aactatcacg gcgctttgga tgccatggaa tatctcttca aattaggaca
     3421 tcaacgaatc ggattcatga gcgggcgcga aggcaatgtc agtggagata ggcgcttcca
     3481 gggatataaa aatgccttaa aaaatgcagg cattgaattc gatagttcac tggttgtgct
     3541 gggcgacttt gttcaaaaaa ctgcatacaa aaatgcattg aaattgctgc aaatagaaga
     3601 ccgcccaaca gcgatttttg ctgcaaacga ccagtctgct attggtgtgt atcaggcagc
     3661 agaagaactt gggatgaaca ttccagcgga tctatctgtg attgggtttg ataatatttc
     3721 agaagcaaaa tacctgggcc tcacctctgt ggatcagttc cttgcagata tgggttacat
     3781 ggccgtgcaa atgttgatca agttaattga aggcgaacct ttagagtttg atgttcataa
     3841 gatgcagaca aagctagtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     3961 ttactcgcaa atttcatgta cttgaaatgc gagcgtccga tatctgaatg acatcggacg
     4021 ccaagaagtc gaagcccgac tctatggaag gatgcggact tcaacttgga aatataccac
     4081 cataattatt aacaaggaga acgaagatga aacgattatc ttacatctta tctctgctgg
     4141 ttgtcgttag catgttctta gcagcatgcg caccagcagc gactgaagaa gcccccgctg
     4201 ccactgaagc agccgctgaa gctactgatg cccctgcagc cactgaagct gcccctgctg
     4261 aattatctgg tactttgaat gtttggtcgt tcaccaatga aattcgcacc atggcgatcg
     4321 cattcgaagg tgttcacccg aatgttgatg ttgtttacac catgatcccg atgaccaatg
     4381 gtgaatacca gaccaaggtt aaggctgctg ccggtaccgc ggattccccc gatgtggtcg
     4441 cgcttgaagc ggctttcgtc aaggaatggg ttgaatctga tcttttggct gaccagagcg
     4501 acttgctgcc tttggctgaa gaactcaaga ccttcccctc tgttgtagaa gttggtacgt
     4561 atgatggcat caccaaggcg tattcttatc aggcgacccc cggcgcgttc ttctatcgcc
     4621 gcagcattgc caaggaatgc ctcggcaccg acgatcctga tgctgtgcaa gccatggttg
     4681 ctgacctcga taagttcgtt gaaaccgctg ccaagatcaa agaatgcggc accggcgatt
     4741 acttcaccgt gggtacctct ggtgaaatgt tcaacccctt cctcgccaat cgcgaacagc
     4801 cctgggttgt ggatggtgaa ctcgtgatcg acccgatggt cttggaatat gtcaagtttg
     4861 ccaagctcat gcgcgagaac ggctacgaat ctcaggctac tcagtggagt gaaggttggt
     4921 tcgctggcat gaacgatact ttgaaggatg ccaacggcac cccgaagaag gtcttcagct
     4981 acttcctccc cacttggggt cttccttacg tgttgatccc caactccaag tctgacacca
     5041 ccgacaccgg cggcgactgg gcgatgatca atggtcctat ggcgtatcaa tggggtggta
     5101 cttgggttgg cgctttggaa ggcagcccca atgctgaatt ggccaaggaa ttcatcaagt
     5161 tcgtagctct taacgaagat aacctgacca actgggcaac cggcgtttac accaacgaat
     5221 acctcaaggc gatcgatcct gaaaccccag atgatcaagc tcaggctcct ggcgactttg
     5281 tttccagcca ggtcgtggtt gagaagatca ccagcacctt cgatggttca gatcttgcca
     5341 aatggctcgg cggacagaac tcctatgccg ctttcgccaa ggctgctcca gatgtcaacg
     5401 gtgctttgtt gaccggttct gacgatgcca ttcagcgtgc cctcaatgat ccgctcgcca
     5461 gctacctcaa tggcgacatc actgaagctg aaatgtacac ccagtggctt gatgcagttc
     5521 gcaatgagtt cccggatctc gttatccctg aacctcctgt cactgaataa gagaatgtga
     5581 agtagtggtt gggtcagtgc aaaacactga cccaacccgt cagaacacca ctgacaattt
     5641 ttattttgtt gaggagacgt catgggactt atcaaactta agaaacatca atacgggtat
     5701 atttttattg caccgttcat tattggtttt cttgccttcg gcttataccc ggtctataac
     5761 actttggcat tgagctttac tgatgcaact ctaatgacca aaagtgctca ttttgtaggt
     5821 ttcaaaaatt ttgagcggtt gtttgctgat gacgtcttcc ttggtgctgt aaagaatacc
     5881 tggttacttt gggcgctgaa ctttattcca caggtcggga tcgccatgtt gttatccgta
     5941 tggttcaccg atgcaagact taagatcaaa ggtgtggggg tatggcgggc ggttttcttt
     6001 ttgcccaacc tgctggtacc tgcatctgta gcggctttat ttttcgcctt cttttctttt
     6061 tacggacctg tcaatcaatt tatggtgcgt tttggttttt tgcccgaagc catgcattac
     6121 ctccaaagcg taccggttac gcgcggattg gttatcttca ttcaatggtg gatgtggttc
     6181 gggcaaactg tcatcatcgt catggcaggg atgacgtcca tccccgtttc attatatgaa
     6241 gctgccatgg tagatggcgc aaccaccagc caaatgttcc gtcgtatcac tctgcctttg
     6301 ctcaagccca ttttgatcta tatctttgtc acatctcttg tgggcggaat gcaaatgttc
     6361 gatattcctt tccttctcac ggatggaaga ggctcaccca atagctccat tcaaaccaac
     6421 aacatcttga tgtacatgaa gttcgccagt agtaagggac atattggtgc agcttcatcg
     6481 gttggtgtgc tggtatttct tatgaccagc atttgtgcac tgggaatttt ctacttcttg
     6541 cgcgaaagag atacagtttc agaaaaaccc aagaagtctc tctttggaaa acgttcgtga
     6601 aagtgataaa gccatgttga acaattacaa attcacaacc accgtccttc gaattcttgt
     6661 ttattttatg ctgttcgcgt tactggtcgt tacgatcgtg ccgatctggc tattgatcgt
     6721 caatgcaacc cgctcaacca ccgagatcca acagggtctc agcattatgc ccagtacgca
     6781 tcttattgaa aattacaaca tcctccttgg aaaagggttg aaccttccca gagggtttgc
     6841 aaatagcacc tttttagcgg tatcgtccac agtggtatct gtctattttt ccatgttgac
     6901 tgcttatggc attgtggtct atgatttcaa aggcaaacat gccttctcaa atttcattgt
     6961 agtgttggtc atgattccca tgcagctctc gatcatcggc ttttatcagt acatgtcaaa
     7021 actggggctg acagataact acgcatctct gatcctgccg ctgatagcca atgcaggcgg
     7081 ggtgttcttt ggaaaacaat atttagaatc catgatcatt caagacttga tcgatgctgc
     7141 tcgaatcgac ggcgccagtg aaatggcaat attccatcaa gtgatgatgc cgcttgccat
     7201 tcccggtgcg gtgaccatgg gtatctttgc cttcattaca tcttggaata acttcttcaa
     7261 cgccttcatc ctgatctctt cgatcgaaaa atacacactt cccatgttgg tgcaaaccct
     7321 gcgcggagat gtgtatcgaa ccgaatttgg cgccatttac cttggcttgg cggccaccat
     7381 tgttccgatc attcttcttt atattttctt ctcacgatac gtggttagcg gcattgccat
     7441 tggagctgta aaggaataaa gcgcctcata ctttaacttt tcttgttcta ccctcttaca
     7501 ctttaccctc catcttagtt ttgcttcatt catttgttct atctccttat gcgttccccg
     7561 cctccatgtg gaggcggggc tgggagagta ttgctaaaaa tcagtaaaaa ccaaggagta
     7621 atctaatgtc caagtattcc ccatcccctg aaaacaagtt caccttcggt ctgtggaccg
     7681 ttggcaatgt tggacgcgac ccattcggta gcccggttcg cgatggcaaa tctcccgcag
     7741 acttggttcg cctgctcgga gaagttggcg catacggtgt caactttcat gataacgacc
     7801 tgattcccat tgatgctacg ccctctgaac gagatgccat tctgaaggaa ttcaagaagg
     7861 cgcttgccga tacaggcatc atcgtgccga tggcaaccac caatttgttc tctgaaccca
     7921 ttttcaaaga tggtgcattt acatcaaacg actcaaaagt ccgcgcatat gcgttgcaga
     7981 agaccatgaa cgccatcgat cttggcgtgg aactcggtgc caaaacctat gtattctggg
     8041 gcggtcgcga aggaacagag agtgacgcca ccaaagatcc aatcacctcc atcaaacgct
     8101 cgcgcgaagc gatgaacttc ctctgcgaat atgtattaga caaaaaatat gacctcaagt
     8161 ttgcgctcga agccaaaccc aatgaaccgc gcggcgacat ttacaactcc accactgggc
     8221 atatgttggc attcattgaa accctcgatc atcccgatat ggtcggcgtc aaccctgaag
     8281 tggcacatga acacatggca gggctcaact tcgtacacgg cgtagctcag gcgttggaag
     8341 cgggcaaact cttccacatt gacttgaacg accaagcctt cggtcgctat gatcaagact
     8401 ttcgctttgg tgcggtcaac atcaagagcg ctttcttcct cgtcaaatta ctcgaagaca
     8461 ataaatacaa caatccacgt cactttgatg ctcatgcata tcgcaccgaa gactacgaag
     8521 gggttaaaga tttcgcgcgt ggctgtatgc acacctatct cgttcttaaa gaaaaagccg
     8581 cacaatggaa tgcggataaa gagatccaat ctctcgttgc agagatcaat gcagatgacg
     8641 gttccatggc gcaatacttt ggcaagtact cctcagccaa agccagcgct ctcaaagctc
     8701 aagccttcga ccgcaatgcc atcgcctctc gcggattgaa atacgaacgc ctcgaccagt
     8761 tgaccatcga cgtactactt ggcgtgcgat aacgtaggag cccttgaatg agtaactcga
     8821 atatcgcgga aaagcccatc tatcttgata cgtcgcgtcc acgggaagaa cgtgtaaaag
     8881 acttgatcag ccaccttact ttggaagaaa aagtggggct gatgagtcac ccaaccaatg
     8941 ggattccgcg cctgaacatc cctgcataca actactggaa tgaagcgtta catggcgttg
     9001 ctcgtaatgg aagagccact gtttttccac aagctattgc catggctgcc acgtgggata
     9061 aggacctgat ccacaaaata ggcaatgcca tcagtgatga aggtcgcgcc aaataccatg
     9121 ctgctttaaa acgcaatggc tacacccaac aatatcaagg cattaccttc tggtcgccaa
     9181 atgtgaatat atttcgtgac ccacgttggg ggcgcggaca agaaacctgg ggcgaagatc
     9241 ccttctttac cggtgaaatg gcggctgagt tcgtcaaagg cttgcaaggc aatgacccca
     9301 cctatctcaa aaccgccgcc tgtgcaaaac actttgctgt gcattctggt ccagaaaaag
     9361 accgccactc cttcaatgcc attgtaacca aacgtgagtt atacgacacc tacctgcctg
     9421 ctttcaaaaa acttgtcacc gaagccaaag tggaagctgt gatgggtgcc tacaaccgca
     9481 ccttggacga agtgtgttgt gccagcaaac ttctgatcga agacatcttg cgcggagaat
     9541 ggggctttga cgggcacttt gtttcagact gcatggcgct gtcagatttt catctcaacc
     9601 acaaggtcac gaaagatgcg gcggaatccg ctgcactggc gttgaaatat ggctgcgacc
     9661 t
//
LOCUS       DAOQFT010000012         9565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3350_length_9565_cov_6.902524, whole genome shotgun sequence.
ACCESSION   DAOQFT010000012 DAOQFT010000000
VERSION     DAOQFT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_9565_cov_6.902524"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /gene="fabF"
                     /locus_tag="PLR93_00545"
     CDS             <1..1267
                     /gene="fabF"
                     /locus_tag="PLR93_00545"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNH25471.1"
                     /translation="FFIVGGEMQERIVITGMGTVNPLGLNAQESWTNAINGVSGVAPI
                     AQFDSHEAKLQVHIAAEVKNFKAENFMDAREARRRDRFEQFAIAASKEALSSSGLQVT
                     EKNAGRIGIIISSAIGGLYSLQEATLTNYKEGPRKVSPFMIPMLMANGGAGMAAIEFG
                     IKGPCFSVASACASGADGIGTALMMLRTGMIDAALAGASEATITSTGVAAFDRVGAMS
                     RRNDNYSMTPQPFDKNRDGLVMGEGAAVLVLERESDAKARGANILAELAGYGATADAF
                     HVTAPHEHGAGGAAAIRMALDSAKANIDEVGYINAHGTGTSLNDQAETRAVKAAFGDL
                     AYKTPISSTKSMTGHMMGATGALEVIFCVNAIRDGILPPTIHYETPDPECDLDYIPNK
                     AREAKISLAISNAFGFGGHNAVLAIRKYS"
     gene            1353..1700
                     /gene="rplS"
                     /locus_tag="PLR93_00550"
     CDS             1353..1700
                     /gene="rplS"
                     /locus_tag="PLR93_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNH25472.1"
                     /translation="MADLIKAVEAKDNEKIPQLAAGDTVSVHVKIKEGERERIQEFKG
                     LVIRLRNSGNNASVTVRRMASNGVGVERTFLLHSPRIDKIVVERHGKVRRAQLYYMRE
                     RTGKSARIKEKFS"
     gene            1811..2521
                     /locus_tag="PLR93_00555"
     CDS             1811..2521
                     /locus_tag="PLR93_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNH25473.1"
                     /translation="MTKPLIGITTFNRNNDYGKPMSGVQHTYIRAVVQGGGIPILIPA
                     ILDEEDRNDLYARVQGVLFTGGGDVSIKYFNGVDHPKIAEVDESRDITEISLLKKTVE
                     DGKPFLAVCRGVQVMNVALGGTLYTHIPDQFPNALHHDNEEFTEIIHPVNINEDSRMA
                     EIFGETLLNVNSLHHQGLKDVASPLKVVGHAPDGMIEAVELPDHPYAMGVQWHPEWLT
                     DQPVMRRLFKSFVDASLQ"
     gene            2522..3358
                     /gene="murI"
                     /locus_tag="PLR93_00560"
     CDS             2522..3358
                     /gene="murI"
                     /locus_tag="PLR93_00560"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNH25474.1"
                     /translation="MPGFGDMNLNPIGVFDSGVGGLSVLREIRNLMPNEDILYFGDQG
                     HVPYGPRPMEQIQDFSEGITRFLLKERSKIIVVACNTASAAALKYLRQAFPNVQFVGM
                     EPAVKPAAEKTQTGKVGVLATPATFQGALYASVVERFANGVELFQNTCNGLVQQIEKG
                     NLDGEETRKILEGALHPMLEKRIDTVVLGCTHYPFVIPLIEKIVGENVRVIDPAPAVA
                     KQVKRLLEAGEALNQKEQLGSTRFMTSGDVEVVKSSLKILLQLEREVEAVTWLNDREI
                     AA"
     gene            complement(3324..3815)
                     /locus_tag="PLR93_00565"
     CDS             complement(3324..3815)
                     /locus_tag="PLR93_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNH25475.1"
                     /translation="MLEKRPNSLLKFFFKTPLFMHKLGLGGWERLIGAQWMFLTTIGR
                     KTGKCRETLVDVMDYDSATDTYFIEAAYGTRADWYKNIQAHPVFEAQVGRRKFNASAK
                     ELNSQDTGETLVQFFRKKPAYTRSVMAMVGLKFKDENELRALGDKLTLLAVTPLSHDR
                     SAK"
     gene            complement(3808..4578)
                     /locus_tag="PLR93_00570"
     CDS             complement(3808..4578)
                     /locus_tag="PLR93_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNH25476.1"
                     /translation="MQIKGLDTLARHVPELNSSSGRMKIGVTFFAIFGLTTFYFIITD
                     QIPTWTLDSQIVVTALGYLILSRVFRQKKAFIEKHKEQAFAVAFKQFTVPSLAIFSAL
                     IIHIAYMNGPKFTQPTLTTILTWLGWVCILIGTPLFIRALTILGADHATMLYVYFPGG
                     ELTHSSIYNIVRHPMYAGMLRICIGLACLNRGIFALTFVIILPILVFGWIRLIEEKEL
                     IERIPNYIEYRKQVPAFFPYPKQIFRFFKFVLTGQSYA"
     gene            complement(4608..5018)
                     /locus_tag="PLR93_00575"
     CDS             complement(4608..5018)
                     /locus_tag="PLR93_00575"
                     /inference="COORDINATES: protein motif:HMM:NF023629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3601 domain-containing protein"
                     /protein_id="HNH25477.1"
                     /translation="MDNPFPTSWNIIAVLMGLFFITFLWGFLRILRARDIKNSQLIQS
                     RPSAPPPLPTPETEGTVYGLYPGWEYIVVQSFTDYYGNIFEVNQKLKFKGRHFLPYQG
                     GHTIMFEECSLYLQEDQNKNILDNFSSYIIKIKQ"
     gene            complement(5108..5722)
                     /gene="cyaB"
                     /locus_tag="PLR93_00580"
     CDS             complement(5108..5722)
                     /gene="cyaB"
                     /locus_tag="PLR93_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNH25478.1"
                     /translation="MSGQETEVKFFVQDLKRVEMRLQELEAHLIQPRVHEVNLRFDNE
                     AGELRKNFKVLRLRQDIEAKFTFKGPSEERAGGVLSRKEIEFSVGNFEAAKEFLELLG
                     FVPVVFYEKYRTTYEFLGVHIMLDELPYGTFVEIEGEDIEALQKVANLLGLNWNAMVR
                     AGYHALFERITEKYNLEYGQLSFEALKSIIATAEDFGITYSDIN"
     gene            complement(5725..6222)
                     /locus_tag="PLR93_00585"
     CDS             complement(5725..6222)
                     /locus_tag="PLR93_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNH25479.1"
                     /translation="MKYITTIEDKQFEIEVVDDHHVRVGGRLLEVNFESVSGQPVYSL
                     ILDGKSYESYVYPGDEDWQVLLRGRQFQAKVEDEREKRLKAAAGGGVAEGGEFHLKAP
                     MPGLVVAIPIAEGQEVKKGEVLVILESMKMQNELKSPRDGTVSHIKVKAGESVEQKQV
                     LLNLH"
     gene            complement(6240..7748)
                     /gene="accC"
                     /locus_tag="PLR93_00590"
     CDS             complement(6240..7748)
                     /gene="accC"
                     /locus_tag="PLR93_00590"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNH25480.1"
                     /translation="MFKKVLVANRGEIAVRILRACRELGLETVAVFSEADRNALHVRY
                     ADEAYLLGPAPSRESYLRTDKILDVVKRSGADAIHPGYGFLAEREDFAAKCADLGITF
                     IGPKPSAIAAMGDKAEARATVIKAGVPVVPGTEDVGNMTNDDLLRIAPTIGFPLLIKA
                     TAGGGGKGMREVRSLEEMPTLLESARREAESAFGDGNVYLEKLIEGARHIEFQIIADE
                     HGNVIHLGERECSIQRRHQKLVEEAPSPFLDEELRKQMGDVAVKAAQAVDYVNAGTIE
                     FLVDKDRNFYFLEMNTRLQVEHPVTELVTGIDIVAEQLRIARGRQLSYTQEQVQFKGH
                     AIECRVNAEDPFNNFIPSTGRITHSIIPTGPGVRVDTGVYPGFEITPYYDPMIAKLIV
                     WGETRAQAILRMRRALEEYRIVGVRTNIPFHQTLMDSHRFMGGQFDTRFVEERFSMEA
                     AATLDEAQAEIAAILATLVSHKETERSAQIVRGNERDTSNWKWVGRWERMHR"
     gene            complement(7767..9317)
                     /locus_tag="PLR93_00595"
     CDS             complement(7767..9317)
                     /locus_tag="PLR93_00595"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNH25481.1"
                     /translation="MSETLKIQQLNDLKAQSRLGGGQARIDAQHKKGRLTARERLDLL
                     LDRGSFREVDPFVVHRTTDFGLDEQKIMSDSVVTGWGTIDGRLVYVYSQDFTVFGGSL
                     GEVHAEKICKIMDMAMKNGAPVIGLNDSGGARIQEGVVALGGYADIFLRNTMASGVIP
                     QISAIMGPCAGGAVYSPALTDFIFMTRNTSYMFVTGPDVVKAVTHEEVTQEELGGASV
                     HSEKSGVCHVAADSEADTLYLIRKLMSYLPQNNMEDSPAAPTSDDPLRMEEALNSLVP
                     DDANKPYDIKEVIRLIVDDGNFFEIHENYAANIVVGFARLGGQSVGIVANQPAVLAGV
                     LDIDASEKGARFVRFCDAFNIPIITFEDVPGFLPGTFQEHHGIIRSGAKLLYAYCEAT
                     VPKLTVITRKAYGGAYCVMSSKHIRSDLNLAWPTSEIAVMGPDGAVNIIFRRELEAAE
                     DPVKRKAELVAEYKEKFANPYVAASRGYIDDVIEPKETRPRLINALAMLSNKRDSNPA
                     KKHGNIPL"
     gene            complement(9283..>9565)
                     /locus_tag="PLR93_00600"
     CDS             complement(9283..>9565)
                     /locus_tag="PLR93_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25482.1"
                     /translation="TLIVFASSFWLMIGIATLFSIWQEKPWSKNLLLGAAAGYTVWYW
                     SERLIWQNPRPNIPFTIVINFACLIVIYFASKSLSREAYERNTENPAIE"
BASE COUNT         2445 a         2193 c         2399 g         2528 t
ORIGIN      
        1 tttttttatc gtcggaggcg agatgcagga acgtatcgtg attacaggca tggggactgt
       61 aaacccactt ggtttgaatg cacaggaatc atggacgaac gcgattaatg gggtgtctgg
      121 tgtggcgccg atcgcacagt tcgattcaca tgaagcaaag ttgcaagtac atattgcggc
      181 agaagtgaaa aacttcaaag cagaaaattt tatggatgcg cgcgaagctc gacgccgcga
      241 ccgctttgaa cagtttgcca ttgccgcttc aaaagaagct ctgtcgagtt ctgggttgca
      301 ggttaccgag aaaaatgctg ggcgtattgg cattattatt tcatctgcaa tcggtggttt
      361 gtattcattg caggaagcca ctctcacaaa ttacaaagaa ggtccgcgta aagtaagccc
      421 gttcatgatt cccatgttga tggcgaatgg tggggcaggc atggcggcaa ttgaatttgg
      481 aattaaggga ccatgttttt ctgtggcttc ggcttgtgct tctggtgcag acggaattgg
      541 tactgcgcta atgatgctcc gcacgggcat gattgatgct gcgctggcag gagcttctga
      601 agcgacgatc acatctacgg gtgtggctgc atttgaccgc gtaggcgcta tgtctcgccg
      661 aaatgacaat tattcaatga ccccccaacc gtttgataag aatcgggacg gtttggtgat
      721 gggcgaaggg gcggctgttc tcgtacttga acgtgagtcg gatgcgaaag cacgcggtgc
      781 aaatattctg gctgaattag ccgggtatgg tgccactgct gatgctttcc atgtgacggc
      841 tccgcatgaa catggggcgg gcggtgcggc agcaattcgt atggcattag actctgccaa
      901 ggctaacatt gatgaagttg gttacattaa cgctcatggc accggaactt cgttgaacga
      961 ccaagccgaa acccgcgctg ttaaggcggc atttggtgac ttggcgtata aaacgccaat
     1021 ttcctcaacc aagtctatga cggggcatat gatgggtgct acgggtgctt tggaagtgat
     1081 tttctgtgtg aatgccattc gagacgggat tttgccgcca accatccact acgaaacccc
     1141 cgaccccgaa tgtgacttgg actatattcc gaacaaggct cgtgaggcga agatttcgct
     1201 ggcaatcagc aatgctttcg gttttggcgg gcacaacgca gtgctggcaa ttcgaaaata
     1261 ttcctgattt ttgcttgtga tttgagatta tccgtgctat aatccgccct cgcgtatatt
     1321 ggaaacctaa tcataaagta aggaagaacg aaatggcaga cttgattaaa gccgtagagg
     1381 caaaagataa cgagaagatc ccccagcttg ctgctggcga cactgtgagc gtccacgtga
     1441 agattaagga aggcgaacgt gagcgtattc aggaattcaa gggcttggta attcgcctgc
     1501 gaaacagcgg caacaatgct tcagtcactg tgcgccgcat ggcgtcaaat ggcgttggcg
     1561 ttgaacgaac cttcctgctc cactccccgc gcattgacaa gatcgtggtg gaacgccacg
     1621 gtaaggtccg ccgcgcccag ctctactaca tgcgcgaacg caccggcaag agcgcccgca
     1681 tcaaggaaaa gttctcctag tttttcctta ttgaatcccg atggcaaccc catcgggatt
     1741 tttttgttta taaccgtttg tttggctgtg gtatgcttat attcgtaaat ttcttgtctt
     1801 ggaggaacct gtgaccaaac cattaatcgg catcacaact ttcaatcgca ataatgatta
     1861 cggcaagcct atgtctggcg tgcaacacac ctatattcgt gcggtggttc aaggtggggg
     1921 cataccgatc ttgatcccgg cgattttgga tgaagaagac cgaaacgatt tgtatgctcg
     1981 ggtgcaaggt gttttattta caggcggcgg agatgtgtct attaaatatt tcaacggcgt
     2041 agaccacccg aaaattgcag aggtcgatga gtcgcgcgat atcactgaga tttcattgct
     2101 gaaaaaaaca gtggaggatg gaaagccttt tctcgcggtg tgccgaggtg ttcaagtaat
     2161 gaatgtcgct ttaggcggca ctctgtacac acatatccca gatcaattcc ctaatgcgtt
     2221 gcatcacgat aacgaagaat ttactgagat tattcaccca gttaacatta acgaggattc
     2281 tcgtatggca gagatttttg gggaaacttt gttgaacgtc aatagcttgc accatcaggg
     2341 attgaaggac gtcgcctcac ccttaaaagt agttggtcat gctccagacg gcatgatcga
     2401 agctgtagaa ttgccagacc atccgtacgc tatgggcgtt caatggcacc ccgaatggct
     2461 caccgatcaa cctgtgatgc gccgactgtt caaatcgttt gtggatgcgt ctcttcaata
     2521 aatgccgggt tttggcgata tgaatttaaa ccctataggt gtgtttgatt ccggtgtagg
     2581 tggactttct gtcttgcgag agatcagaaa cttgatgcca aatgaagaca ttctatactt
     2641 tggcgatcag gggcacgtcc catacggtcc gcgcccgatg gagcagatac aggatttttc
     2701 tgagggcatt acgcgctttt tgctgaagga aaggtccaag atcattgtgg tggcgtgtaa
     2761 tacggcttcg gctgcggcat tgaaatattt gcgccaggct tttcctaacg ttcaattcgt
     2821 aggcatggaa ccggcggtta agccagcagc agagaaaaca cagacgggga aagtaggcgt
     2881 tctggcaaca ccggctacgt ttcagggagc gctttatgct tctgtcgtgg agcgtttcgc
     2941 aaatggcgtg gagttatttc aaaatacctg caacggactt gtgcaacaga tcgagaaggg
     3001 gaatttggat ggtgaagaaa ctcgcaaaat cttagaaggt gctcttcacc ccatgcttga
     3061 aaagaggatc gataccgttg tgttgggatg cactcattac ccttttgtga ttccgttgat
     3121 cgaaaaaata gtgggcgaaa acgtgcgggt gatagaccca gctccggcag tggcaaaaca
     3181 ggtcaaacgt ctgcttgagg cgggcgaagc tttgaaccaa aaggagcaac ttgggagcac
     3241 acgctttatg acatcgggtg acgtcgaggt ggtcaaatcc agtttgaaaa ttttgttgca
     3301 gcttgagcgg gaagtcgaag cggttacttg gctgaacgat cgtgagatag cggcgtgacc
     3361 gctaagagag ttagcttgtc gcctaatgcg cgaagctcat tctcatcttt gaatttcaac
     3421 ccgaccattg ccatcacgga gcgggtataa gcaggctttt ttctaaaaaa ctgaaccaac
     3481 gtttcgccag tgtcttgtga gtttaactct ttagcactgg cgttaaattt tcgcctaccg
     3541 acctgtgctt caaaaactgg gtgcgcttga atgttcttat accaatctgc acgggtgcca
     3601 taggcggctt caatgaagta ggtgtctgtg gcagagtcat agtccatcac atctacaagc
     3661 gtttcgcggc atttgccggt ttttctgccg atggtggtga gaaacatcca ttgcgcccca
     3721 atgaggcgct cccaaccacc taaaccgagt ttgtgcataa agagaggagt tttgaagaaa
     3781 aacttcagta gagagttggg gcgtttttca agcatacgat tgacctgtta gaacaaattt
     3841 gaaaaagcgg aatatttgtt tcgggtaggg aaagaatgcc ggcacctgtt ttcgatattc
     3901 aatatagttg ggaatgcgtt ctattagttc cttttcttct atgagacgga tccaaccaaa
     3961 aacaaggatg ggtagaataa ttacaaaggt cagcgcaaaa attccgcggt tgaggcacgc
     4021 taacccaatg cagatgcgaa gcatgcctgc atacatgggg tgacgaacaa tgttgtagat
     4081 acttgagtgt gtcagctcgc cgcctggaaa atacacatat agcatagtgg catgatctgc
     4141 acccaatatg gtaagcgcac gaataaatag gggcgttcca ataagaatac aaacccagcc
     4201 aagccaagtg aggattgttg taagtgtagg ctgggtaaat tttggtccat tcatgtaagc
     4261 gatgtgaata attaatgcag aaaaaatagc aagacttggc actgtaaatt gtttgaatgc
     4321 cacggcaaag gcttgctctt tgtgcttttc aataaaagct ttcttttgcc tgaagactcg
     4381 actaagaata agataaccca atgcagtgac gacaatttgg ctatcaagag tccatgtggg
     4441 gatttggtcg gtgatgatga aataaaaggt ggttaaacca aatatggcga agaatgttac
     4501 gccgattttc atccgccctg atgatgaatt taattctgga acatgccttg ctaaagtatc
     4561 taaacctttg atttgcatgg caatctcctt gtctgaaacc tgttagttta ttgtttgatt
     4621 ttgataatat aactcgaaaa attatcgaga atattcttgt tttggtcctc ttgtaaataa
     4681 aggctacatt cttcaaacat aatcgtatgc ccgccttgat acggtaaaaa atggcgtcct
     4741 ttgaatttga gcttttgatt cacctcaaag atattgccat agtagtctgt aaaagattgt
     4801 actactatgt attcccagcc tgggtaaagt ccatagacag ttccctctgt ttctggagtt
     4861 ggtaaaggtg gcggagcgga cggtctggat tgaataagtt gagaattttt tatgtctctt
     4921 gctcgtaaga tgcgcaagaa tccccataaa aacgtgatga aaaacagccc catgagaacg
     4981 gcgatgatgt tccatgaagt agggaatgga ttatccatga tccatatcac catggcaaag
     5041 aaaacaaaac tgacaaaaag caatagagca gtgaagagcg ggttaggcgt agaacggttc
     5101 ataaattcta gtttatatca gaataggtga ttccgaaatc ttcagccgtg gcaatgatgg
     5161 actttaatgc ctcaaaactc aactgaccat attcaaggtt gtacttttct gtaatgcgct
     5221 caaacagtgc gtgatacccc gccctgacca tagcgttcca gttcaaccca agaagattcg
     5281 ctaccttttg aagtgcttca atatcttcgc cttcgatttc aacaaaagta ccataaggca
     5341 attcatctaa cataatgtgc acgccaagaa attcataggt tgtgcgatac ttttcgtaaa
     5401 agacaactgg cacgaaacca agtaattcta gaaattcttt ggctgcttca aaattgccaa
     5461 cagagaattc aatctctttc cggctgagta cgccacccgc gcgttcttca cttggacctt
     5521 tgaacgtgaa tttggcttcg atatcctgtc gcaggcgaag taccttgaag ttttttctta
     5581 attccccggc ttcattatcg aatcgtaaat tgacttcatg gacacgcggc tgaattaaat
     5641 gcgcctccag ttcttgaagg cgcatttcaa ctcgttttag gtcttgcaca aaaaacttaa
     5701 cttcggtttc ttgtccgctc atggttagtg tagattcagc aacacttgct tttgctcaac
     5761 gctctcgcca gccttgacct tgatatggct caccgtgcca tcgcgcggtg actttagctc
     5821 attctgcatc ttcattgact caaggataac aagtacctct ccctttttga cttcttgccc
     5881 ttcggcaata ggaatggcga caaccagccc tggcattggc gctttgagat ggaactcgcc
     5941 tccttctgcc acgcctccgc ctgcagccgc tttcagacgc ttttctcttt catcctcaac
     6001 tttggcttgg aattgtcgtc cgcgtagtaa gacctgccag tcttcatcac cggggtatac
     6061 atacgactcg taggatttgc catcgagaat taaagaatag acaggctgac cgctaaccga
     6121 ttcaaagttg acttcgagca ggcgtccgcc cacacgtaca tggtggtcgt cgacaacttc
     6181 tatttcaaat tgcttatctt ctattgtagt tatatatttc atgccatagc ctcttctaat
     6241 tagcggtgca tgcgttccca gcgaccgacc cacttccagt tgctggtgtc gcgctcgtta
     6301 ccacggacga tctgagcaga gcgttccgtc tctttgtgag atacgagcgt agcgaggatg
     6361 gctgcaatct ctgcctgtgc ttcatccaag gtggcagcgg cttccatgga gaagcgttct
     6421 tccacgaagc gggtgtcgaa ttgcccaccc atgaaacgat gcgagtccat caaggtttgg
     6481 tggaagggga tattagtgcg aacacccacg atgcgatatt cttccagagc acggcgcatg
     6541 cgcaaaatag cttgagcgcg tgtctcgccc caaacaatca gtttggcaat cattggatcg
     6601 taatatggtg tgatttcaaa accggggtac acacctgtgt ccacacgaac gccgggtcca
     6661 gtagggatga tgctatgggt gatgcgaccc gtggatggga tgaaattgtt aaacgggtct
     6721 tctgcattga cacggcactc aatcgcatgt cccttgaact gtacttgttc ctgtgtatag
     6781 ctgagttgac gtccacgggc gatacgcaac tgttcagcga cgatgtcgat accggtgacc
     6841 aactctgtca caggatgttc cacctgcaaa cgggtgttca tttcaaggaa gtaaaaattg
     6901 cggtctttat caacaaggaa ttcaatcgtc cctgcgttga catagtccac agcttgcgcg
     6961 gctttgacag ctacatcgcc catttgcttg cgcaattctt catcgagaaa gggtgagggc
     7021 gcttcttcta ccaatttttg atggcgacgt tgaatggaac attcacgttc gcccaagtgg
     7081 atgacattgc catgttcatc agcaatgatt tggaattcga tgtgacgcgc gccctcgatc
     7141 aatttctcga ggtagacatt gccatcgcca aacgcagatt ctgcctcacg cctcgcggac
     7201 tcaagaagag ttggcatttc ttccaagctt cggacttctc tcatgccttt tccgccgcca
     7261 cccgctgtcg ccttgataag aagcgggaag ccaatcgtag gagcaatgcg gagtaagtca
     7321 tcgttggtca tattgccaac gtcttcggtg cctggcacaa cgggtacgcc agctttgatg
     7381 actgtagcgc gtgcttcggc tttgtcgccc atcgccgcaa tagctgaagg tttgggtcca
     7441 ataaaggtga tgccaaggtc tgcgcatttg gcggcgaaat cttcgcgctc tgcaaggaag
     7501 ccgtaccccg ggtgaatagc atctgctcca gagcgtttta caacatcgag aatcttatcc
     7561 gtgcgtaaat aggattcacg tgaaggagcg ggaccaagta gataggcttc atctgcatag
     7621 cgcacatgaa gtgcgttgcg gtctgcttcg gaaaatacag caacggtttc aagccctaat
     7681 tcgcgacaag cgcgcaagat gcggacggcg atctcaccac ggtttgcaac caatactttt
     7741 ttaaacatga ttctctcctc gcctgattac agcggaatgt tgccgtgttt cttggctggg
     7801 tttgaatcgc gtttgttgct caacatggca agtgcgttga tcaagcgcgg acgggtttct
     7861 ttaggttcga taacatcatc gatataccca cgagaggctg caacatacgg gttcgcaaat
     7921 ttctctttat attcagcaac caattcagcc ttgcgcttga cggggtcttc tgcggcttcc
     7981 aactcgcggc ggaagataat gttgactgca ccatcgggtc ccatcacagc gatttcggag
     8041 gtgggccagg caaggttaag gtcactgcgg atgtgcttgg aagacatcac gcaatacgca
     8101 ccgccatacg ctttgcgagt gatgaccgtt aacttcggta cagtcgcttc gcaatatgca
     8161 taaaggagtt ttgctcccga gcggataatg ccatgatgtt cttggaatgt tccaggtagg
     8221 aagccgggca catcttcaaa ggtgatgatc gggatgttga atgcgtcgca gaagcgaacg
     8281 aagcgtgcgc ctttttcact ggcatcaata tcaagcacgc ctgcgaggac agcaggctga
     8341 ttggcaacga tgccaacgga ctgtccgccg aggcgagcga aaccaaccac aatattggcg
     8401 gcgtagtttt catggatttc aaagaagtta ccatcgtcca caataaggcg gatgacttct
     8461 ttgatgtcgt atggtttatt ggcatcatct ggtacaagtg aattgagtgc ttcttccatg
     8521 cgaagcgggt catccgatgt tggtgccgca ggtgagtctt ccatgttgtt ttgcggcaaa
     8581 tagctcatca acttgcggat gagatacaag gtatctgctt cgctatcggc cgcgacatga
     8641 cagacgccgg atttttctga gtgaacactt gcaccaccca attcttcctg agtcacttct
     8701 tcatgagtta cagctttcac cacatcaggt ccggtcacga acatatagga tgtgttgcgt
     8761 gtcatgaaga taaagtctgt gagggcaggg gagtacacgg caccgccagc gcagggtccc
     8821 ataatggcag aaatctgtgg aattacgcca gatgccatgg tattacgcaa gaaaatatcg
     8881 gcgtagccac ctaaagccac aacgccttct tgaatacgtg caccaccaga gtcgttcaaa
     8941 ccaataacgg gggcgccgtt tttcattgcc atgtccatga tcttgcagat tttttcggca
     9001 tgcacttcac caaggctgcc accaaagacg gtgaaatctt gtgaatatac atacacgagc
     9061 cgcccgtcaa tggttcccca gccagtgacg acagagtcac tcattatctt ttgttcatcc
     9121 agtccgaagt ctgttgtgcg gtggacgacg aacgggtcca cttcgcggaa ggagccgcga
     9181 tccaataaaa ggtcaaggcg ttcgcgtgca gttaaacgtc cttttttatg ttgagcgtca
     9241 atgcgggctt gtccaccgcc aagacgagat tgcgctttca ggtcattcaa ttgctggatt
     9301 ttcagtgttt cgctcatatg cctctctcga caaagatttc gatgcaaaat agataacgat
     9361 caaacatgca aaatttataa caatcgtgaa cggaatgttc gggcgtggat tttgccagat
     9421 gagccgctcg ctccaatacc agacggtata tcctgccgct gccccaagga gcagattctt
     9481 actccaaggt ttttcctgcc aaatgctaaa caaggttgct attccaatca taagccaaaa
     9541 tgaacttgca aagacaatca gagtt
//
LOCUS       DAOQFT010000013         9559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3351_length_9559_cov_6.416035, whole genome shotgun sequence.
ACCESSION   DAOQFT010000013 DAOQFT010000000
VERSION     DAOQFT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3351_length_9559_cov_6.416035"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLR93_00605"
     CDS             <1..792
                     /locus_tag="PLR93_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25483.1"
                     /translation="PAVGYDLIDVTSPSPIQIIPIFLDLPPDPATPNTGILPESTLTP
                     LFSIGDTISLRTGVIKDHNGNPVPDGTVVRFSMLLTGEGGGILQQVESITTDGVARAA
                     FGLDKPGLLEIRVASEPAVISEVLQLDVSQTGEVAVTVVVPELTQSSVPTATPEAVEE
                     ADAFVTQEGYPRVSAWSLAMLFVLLGSGALYVIASRFTKRMIATRWSLGMLLGGLFVY
                     NLMAFGFFDLPSWLLEAGISGVLYATLTGQLLGFAAGWAWSRLEK"
     gene            873..>1771
                     /locus_tag="PLR93_00610"
     CDS             873..>1771
                     /locus_tag="PLR93_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNH25484.1"
                     /translation="MESNGKAGKNKVIKAKQPPILFDKTQAIIADLTKKLGGTLVTYF
                     NNPRGAVCHDDVLALFELLEQIGKQEKIYLFLKSSGGNGQASLRIVNLLRQYCEELIA
                     VIPLECASAATMITLGANEIHMGPMAYLTSVDTSLTHALSPIDRDNDRVSVSLDELNR
                     VVKLWQAQRSDSNENPYQQLFQHVHPLVIGAVDRAESLSIMLCKELLAYHIKDEQVGE
                     KIASTLNSKYPSHGYPILYEEAHRIGLKVSRLSPEINSQLLELNELYSEMGQRATTDF
                     DDTHAHGNEILNIWESTDVLVYY"
     assembly_gap    1772..1871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1872..2053
                     /locus_tag="PLR93_00615"
     CDS             <1872..2053
                     /locus_tag="PLR93_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25485.1"
                     /translation="GNEILNIWESTDVLVYYQQDKDWFYRTEERRWISLNDNSSWRRM
                     HKKGNKVEKSILHIS"
     gene            2179..2778
                     /locus_tag="PLR93_00620"
     CDS             2179..2778
                     /locus_tag="PLR93_00620"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HNH25486.1"
                     /translation="MATNIHLKFNDQAPDLTLLDGAGNPVQLSSLWKKQVLVLAFTRH
                     FGCPQCKEMVDQLIDSHQTLTEAGLLLAIVSHASPESAKAFCDQRAPNALCLADPDRT
                     VYKAYGLYRGTLWQTILSPNIWKSNRRLARQKGIKPGVPSPGQDVYQMSGTFVIGMDG
                     KIRLPYYYEDIADHPPVDLLLHGIMGVDWKKPFDSNPLV"
     gene            2792..3952
                     /locus_tag="PLR93_00625"
     CDS             2792..3952
                     /locus_tag="PLR93_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25487.1"
                     /translation="MKTFFLATYLALKEVFRNRGRFLLVALVIALITMLVLFIAALGE
                     GLGNGNRQYIANLDADLIVFLEKSDYIISASRLEVNTARSIRRVEGVADAGAIYTSST
                     EIVSLEEPLKVSMLGVEPGRPGMPTILEGREFRTGEAREAVIDRNVALRSDIKIGDTI
                     RIRSTQGTDDEFYELKVVGLVDGQSYTFQPTIFVPPATWEKIRPQSESDMVSDTPYPN
                     VVAVKLQDPAQIDAMSARLMERIPNIEVADIQTTISNIPGYSAQQGTVQTQAVFTLLI
                     GTLVIGGFFQIQILQKVPQIGVLKAIGSANGMVGLSAVIQIIVVTAMGVGIGSGLTYF
                     FSLGFPPTIPIVFNGVNSLIAVILLLLIGPIGGMVSIIYAVRIEPLKALRLG"
     gene            3955..4683
                     /locus_tag="PLR93_00630"
     CDS             3955..4683
                     /locus_tag="PLR93_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25488.1"
                     /translation="MSDIALEVRDLVKTFSLDGSTINAVDHVSFQVKFGEFVALVGPS
                     GSGKTTMLSILAALLTPTSGQVLIDGQDLSKMSEKERVKLRREKIGFTFQANNLIPYL
                     SARENVEFMLRLNGKLDHASRIRSDELLARLGLAERLHNLPAQMSGGQQQRVAIARAL
                     IHNPALVLADEPTASLDTERAFQVVETFANLIHENGRAGIIVTHDLRMCQYVDRVFQM
                     QDGKLIRTYETKNEIMELATATVH"
     gene            complement(4731..5297)
                     /locus_tag="PLR93_00635"
     CDS             complement(4731..5297)
                     /locus_tag="PLR93_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH25489.1"
                     /translation="MKKYSLILIALLGALACKALTPNPQEVETPVATDEPPSVIVQIT
                     DAPTEETAVTSSTGFTIVRLKPEDGDLATMLESESKKAQALGQMPVVEFDATWCPPCQ
                     AIDQSIGEENELMMNAYDGTYIIKLDVDVWGWDNGKVEMFQFEAIPVYFKLDTSGKPN
                     GEVIDGGAWGEDIPENIAPVMDAFFHNK"
     gene            complement(5320..5838)
                     /locus_tag="PLR93_00640"
     CDS             complement(5320..5838)
                     /locus_tag="PLR93_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25490.1"
                     /translation="MKKIFAFAILSVLLCSACSQNKAYPLKLATFDKNSVSVLIHLEK
                     DADENYFLSATFTPEQGFHLYSKDLPITGANGLGRPTLIEAGVESQYQVTGKLLESVP
                     AEIPEFEPKDLLVYPVGAVTLRLSIELPPGNGWTDDSVKITYMACSDRGCKAPVIGQE
                     IAIRIPSKETFH"
     gene            complement(5935..7278)
                     /gene="glnA"
                     /locus_tag="PLR93_00645"
     CDS             complement(5935..7278)
                     /gene="glnA"
                     /locus_tag="PLR93_00645"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNH25491.1"
                     /translation="MDSKELLKRVKEDNVKFLSLQFTDVLGAVKSVDMPIRHLEDVLK
                     DGAWFDGSSVEGFARIQESDMRLKVDPETYVLLPWSSPESRRARVFCDIYTPSGEPFE
                     GDPRGALKRILKKIDERGWKFNIGPEPEFFLFKGNDGSGNVHPVPHDTGGYFDFSSFD
                     EAVVVRTALMDALDQMGLDVEVGHHEVALGQHEIDFRFADALKAADNVLTLKYTVKAI
                     AAQHGLVASFMPKPVYGINGSGMHCHQSLFDTKTGNNLFFDAKDEAHLSQLAYSFIAG
                     QLEHARALAGVVAPTVNSYKRLVPGYEAPVYIGWAQQNRSALIRIPRYTEGRDKAVRA
                     ELRFPDPSSNPYLAFTVMLAAALDGIDKNMKAPKPLNNINLYHLNKEERAKLGVTELP
                     GSLAESLKELEKNEVLKAALGDYLFEAYMRAKWEEWDEFRLRVTDFEIQRYLETA"
     gene            complement(7345..8277)
                     /locus_tag="PLR93_00650"
     CDS             complement(7345..8277)
                     /locus_tag="PLR93_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH25492.1"
                     /translation="MKTKLWTALIALWIVWGSTYLAIRFAVETIPPFLHASLRFLVSG
                     AILVVWRHMAGDPKPTTSNWKATAIVGTFLLLGGNGFVSLAEQSVPSGIAALMITTSP
                     FWLVLFESLRSGGTKPSWQAIVGLLLGFGGVFVLVGPGEIAGSQHTFETSGVILLVLA
                     PILWSMGSIYSRGADMPKSTLMSTGMQMLAGAGSLFIVSVATGELSGFNFGDVSMRSW
                     WGLIYLITFGSLVGFVSYGWLIQNAPVSLTSTYAYVNPMVAVLLGWLFAGEELNARIA
                     VSSVIIIGAVIMINLARNSKPAPKNEMAIPEEIE"
     gene            complement(8302..9162)
                     /locus_tag="PLR93_00655"
     CDS             complement(8302..9162)
                     /locus_tag="PLR93_00655"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNH25493.1"
                     /translation="MDPITRELQDLEPLQHYRLIEQAIQYIETNVHHQPELAEIASAV
                     GLSEYHFQRLFTRWAGISPKRFMQFLTKEHAKQLLDKSENLLEATHQTGLSSLGRLHD
                     LFVNTEAVTPGEYKSRGAGIDIHYGIHLTPFGKCLIATTDRGICNLSFVNASEGKAID
                     DLVAHWSHANMIEDYQATAPLVNRIFLGAQLDSPLKLHLRGTNFQIKVWEALLSIPTG
                     ALTTYEHIATTIGNPRAVRAVGSAVGDNPIAYLIPCHRVIRKSGEFGNYLYGSARKKA
                     ILAREFVMTE"
BASE COUNT         2394 a         2242 c         2272 g         2551 t
ORIGIN      
        1 cctgcggttg gatatgattt gatcgatgtc acatcgccca gcccgattca gatcattccg
       61 atctttctgg acctgccgcc tgacccggca accccaaata ccggcatcct ccccgaatcg
      121 acgttaactc cgctgtttag tattggcgat acgatttctc ttcgtactgg ggtgatcaaa
      181 gaccataatg gaaacccagt ccctgatggg acagtggttc ggttctcgat gttattgact
      241 ggcgagggcg gtggcatttt gcagcaagtt gaatcgataa cgaccgatgg tgtggcgcgt
      301 gcggcatttg gtttggataa acccggtcta ttagagattc gagttgccag cgaacctgct
      361 gtgatctctg aagtattaca gttggatgtc tcacagactg gtgaagtcgc ggtgacggtg
      421 gtcgtgccag agttgacaca atcttctgtg ccaactgcca cgcctgaagc ggtagaagag
      481 gcggatgcct ttgtcacgca ggaaggctat ccgcgggttt cggcttggtc tcttgccatg
      541 ctctttgttt tgctggggtc tggcgctttg tacgtgatcg cttctcgttt taccaaacga
      601 atgattgcta cacgttggtc gttaggaatg ttgttaggtg gtttgttcgt atataacctg
      661 atggcatttg gcttttttga ccttccaagt tggttgttag aggcgggaat atctggcgtg
      721 ctgtatgcca cgcttacagg tcagttatta ggttttgctg ctggctgggc ttggtcacgc
      781 ctggagaaat agtactatca acttattttg tttctagcgt tcagtgttac catttattgg
      841 attgatatca ttaaggttag gagacgaatt atatggaatc taacggcaaa gctggcaaaa
      901 ataaagtgat caaagcaaag caacctccga ttctgtttga caagactcag gcaatcattg
      961 cagacttgac caaaaaactc ggcggcacgc ttgtcacgta tttcaacaat ccgcgcggtg
     1021 cggtttgcca tgacgatgtg ttggctttat ttgaactgct cgaacagatc ggcaaacagg
     1081 aaaagattta tttattccta aaatccagcg gcggtaatgg acaagcttct ttgcgtattg
     1141 tgaatctttt acgacagtac tgtgaagaac taattgctgt gattcctctg gaatgtgctt
     1201 cagcggcgac catgattaca ttgggcgcga acgaaatcca tatgggaccc atggcatatc
     1261 tcacttcagt ggatacttct ttgacgcacg cattatctcc cattgaccgc gacaacgacc
     1321 gcgtgagcgt cagcttggat gaattgaatc gtgtcgtgaa actgtggcaa gctcaaaggt
     1381 ccgactcgaa cgagaaccct tatcaacaac tctttcagca tgtgcatccg cttgtgattg
     1441 gcgccgtgga ccgtgctgag tctctttcca ttatgttgtg caaagaatta cttgcctatc
     1501 acattaaaga tgagcaagtg ggtgagaaga tcgcatcgac acttaactct aaatatcctt
     1561 cgcatggcta tcctattttg tatgaagaag cacaccgcat tggcttgaag gtaagccgcc
     1621 tttcaccaga aatcaattcg caattgcttg agctcaacga gctttacagc gaaatgggtc
     1681 aacgcgctac aacagatttt gacgacactc atgcacatgg gaatgagatt ctcaatattt
     1741 gggaatcgac cgatgtcttg gtgtattacc annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn natgggaatg agattctcaa tatttgggaa tcgaccgatg tcttggtgta
     1921 ttaccagcaa gacaaagatt ggttctatcg cactgaagag cgccgctgga tttcgctcaa
     1981 cgataatagc agttggcggc gaatgcacaa aaagggtaac aaggtcgaga aatcgatcct
     2041 gcatatctcg taagaatttg aggcacagta gggaactgtg cctctttttt tggttagggg
     2101 agggtaaaaa caactcgttg caaaacctgt acaaaacctg tgcattgtgc taaaatctcg
     2161 tcattcaaaa aggtaactat ggcaacaaat attcacctca agtttaatga ccaagctccc
     2221 gacctaaccc tcttggacgg cgcaggcaac cctgtgcagc tttcatctct ctggaagaag
     2281 caagtccttg tgctggcgtt tacccgtcac tttggatgcc cgcaatgcaa agagatggtg
     2341 gatcaattga tcgactcgca tcagactctg accgaggctg ggttgctgct cgcaatcgtt
     2401 tctcacgcct cacccgaatc agccaaagcg ttttgtgacc agcgtgctcc gaatgcgttg
     2461 tgcctcgctg acccagaccg taccgtttac aaagcctatg gtctttaccg tggcactctc
     2521 tggcaaacca tcctttcccc caatatttgg aagtccaata gaaggttggc gcgacagaaa
     2581 ggcatcaaac ctggtgtacc atcccccgga caggatgttt atcaaatgtc tggcaccttt
     2641 gttattggta tggatgggaa aattcgtttg ccttattact acgaagatat tgccgatcat
     2701 ccacctgtag atttgctttt acatggcatc atgggtgtgg attggaagaa accgttcgac
     2761 tcaaaccctt tggtctagga agtacgaaat aatgaaaact ttttttcttg caacttacct
     2821 tgcgctcaag gaagtgttcc gcaatcgcgg acgttttttg cttgtggcgc tcgtcatcgc
     2881 gttgatcacg atgttagtcc tcttcattgc tgcattaggg gaggggttgg gcaatggtaa
     2941 ccgccaatat attgccaact tggatgcaga cctgatcgtc tttttggaga aatcagatta
     3001 cattatctct gccagccggt tggaagtaaa cactgctcgt tccattcgac gtgtggaagg
     3061 tgtggcagat gctggcgcga tctatacatc cagcacagaa atcgtgtcgt tagaagaacc
     3121 cttgaaggta tcaatgctgg gtgtagaacc aggtcgcccc ggcatgccta ctattttgga
     3181 agggcgtgaa ttccgtaccg gtgaagcgcg tgaagccgtg attgaccgca acgtagcttt
     3241 gcggtcagat attaagatag gcgatacgat tcgaatccgc tccacccaag gcaccgacga
     3301 tgaattttat gaacttaagg tcgttggctt agttgatggt caatcatata cctttcagcc
     3361 cactattttt gtcccgccag ccacatggga aaagattcgc cctcaatctg aatcagacat
     3421 ggtcagtgat acaccgtatc ccaacgtggt agctgtgaag ttgcaagacc ctgctcagat
     3481 tgatgcaatg tctgcccgtt tgatggaacg tatacccaat attgaagtgg cagatattca
     3541 aacgaccatc agtaatattc ctggatattc tgcccagcaa gggacagttc aaacgcaggc
     3601 ggtctttact ttattgatcg gcactcttgt tatcggcggc ttcttccaga ttcagattct
     3661 tcaaaaggtt ccgcagatcg gtgtgttgaa ggcaattggg tctgctaatg gaatggtagg
     3721 gctttcggca gtgattcaaa tcattgttgt gaccgccatg ggggtgggca ttggcagcgg
     3781 cttaacctac ttcttctcgc ttgggttccc tccaaccatt ccgattgtgt tcaatggtgt
     3841 taattcactc atcgctgtga tcttgcttct gttgattgga ccgatcggtg gaatggtctc
     3901 tatcatttat gctgttcgta ttgagccgct caaggcactt aggttagggt aaatatgtca
     3961 gatattgcat tggaagttcg cgatttagtc aaaaccttct cactcgatgg ctcgaccatc
     4021 aacgcagtag atcatgtatc gtttcaggta aagtttggtg agtttgtggc gttggtcggt
     4081 cctagtggtt ccggtaaaac gactatgctc tccatccttg ctgccttact tacacccact
     4141 agcggacaag tcctcattga tgggcaagac ctttcaaaga tgagcgagaa agaacgtgtc
     4201 aaactgcgcc gcgagaagat cggttttacc tttcaggcga ataatttaat tccatatctt
     4261 tctgcccgtg agaatgtgga atttatgctg cgcctcaacg gcaagctgga tcatgccagc
     4321 cgcatccgct ctgatgaatt acttgctcgg cttgggttgg cggaacgctt acacaacttg
     4381 cctgcacaaa tgtctggtgg tcagcagcaa cgtgttgcta tcgcccgggc gttgattcat
     4441 aaccctgcac ttgtacttgc cgatgaaccc accgcttcac tcgatactga gcgtgccttc
     4501 caagtagttg aaacctttgc aaatctcatc catgaaaatg gacgtgccgg tatcatcgtg
     4561 acgcacgact tgcgtatgtg ccaatatgta gaccgtgtct tccagatgca agacggtaaa
     4621 ctgatccgca cgtatgagac taaaaacgaa attatggaac tggctacagc gacagttcac
     4681 taaattggtt ataaataaaa agggctcact tcaaaagtga gcccttttta ttatttgttg
     4741 tgaaaaaagg catccatgac aggtgctatg ttttcgggaa tatcctcgcc ccatgcaccg
     4801 ccatcaatca cttcaccgtt tggttttcca gaagtatcca gcttgaaata tacagggata
     4861 gcttcaaatt ggaacatttc tactttgcca ttatcccaac cccagacatc aacatccaac
     4921 ttgatgatat aggtcccatc gtaggcgttc atcattaatt cgttctcttc accaatagac
     4981 tggtcaattg cctgacaagg tgggcaccag gtcgcatcaa actcaacgac aggcatctga
     5041 cccagcgctt gtgctttttt actttcgctt tctagcatcg tcgctaagtc tccatcctct
     5101 ggcttgagcc gcacgatggt aaacccagta gacgatgtta cagcagtttc ttccgttggt
     5161 gcgtctgtga tttgaacaat cacggacggc ggttcatctg tagcgactgg agtctctact
     5221 tcttgtggat ttggcgttag tgctttacat gccaatgctc ctagaagagc aatcaatatc
     5281 aaactatatt ttttcataag tctcccatct tttatagaat tagtgaaacg tctctttgct
     5341 ggggatacga attgcaatct cttgaccaat aaccggcgct ttacagccac ggtctgaaca
     5401 agccatgtag gtgattttca ctgaatcgtc tgtccaacca tttccgggtg gaagctcaat
     5461 ggacagacgc aaggtgactg ccccaacagg ataaaccaaa agatccttcg gctcaaattc
     5521 tggaatttcg gctggtacac tttcaagtaa tttccccgtg acttgatact gactttcaac
     5581 gccagcttct attaatgttg gacgtccaag tccatttgcc cctgtgatgg gtaggtcttt
     5641 actatataga tgaaaccctt gctctggggt aaacgtagca gaaaggaaat aattttcgtc
     5701 cgcgtctttt tccaagtgga ttaagacgct taccgaattt ttgtcgaaag ttgccaattt
     5761 aagcggatat gctttatttt gagaacaggc actgcataat agaacactta agatagcaaa
     5821 agcaaaaatc tttttcattg aaaataatta taagacaaag tgaatttcaa ggaataaaac
     5881 aaaacggcac tcagaatttt ctgagtgccg ttttgttttg gctagtcaga acgtttatgc
     5941 tgtttctaaa tagcgctgaa tttcaaaatc tgtcacgcgt aggcggaatt catcccattc
     6001 ctcccacttg gcacgcatat aggcttcaaa cagatagtct ccaagagctg ccttcaagac
     6061 ttcgttcttc tctaattcct tcaaagattc agccaacgag ccagggagtt cggtcacacc
     6121 gagcttggcg cgttcttctt tgttcaggtg atagagattg atgttgttta gtggtttagg
     6181 cgccttcatg ttcttgtcga taccatcgag cgcagcggca agcatgacgg tgaacgccaa
     6241 atacgggttg gacgaaggat cagggaagcg caattccgcg cgaacggctt tatcgcgacc
     6301 ttctgtgtag cgcggaatac ggatgagcgc cgagcggttc tgctgagccc aaccgatgta
     6361 cacaggcgct tcataacccg gaactaaacg cttgtatgag ttcacggtcg gagcgacgac
     6421 gccagcaagg gcgcgggcat gttcgagttg accggcgatg aacgaatacg ccaactgcga
     6481 gagatgagct tcatccttgg catcgaagaa caaattattg ccggtcttag tgtcgaacaa
     6541 tgattgatgg cagtgcatac cagaaccgtt gatgccataa acaggcttgg gcatgaacga
     6601 agcgacgagt ccgtgttggg cggcgatcgc cttcaccgtg tacttaagcg tcaggacgtt
     6661 atctgcagct ttgagcgcgt ctgcgaagcg gaagtcaatt tcatgctgac ccaacgccac
     6721 ttcgtgatgc ccaacttcca catcgagtcc catttgatcg agagcatcca tcaaagcggt
     6781 gcgcacaaca acggcttcat caaatgaaga gaaatcaaaa taaccaccag tatcgtgggg
     6841 gacggggtga acattaccgc tgccatcgtt gcccttgaac aggaagaact ctggctcagg
     6901 accaatattg aacttccaac cgcgttcatc aatctttttg agaatacgct tcaatgcgcc
     6961 gcgtgggtcg ccttcaaacg gttcgcctga tggagtatag atatcgcaga acacacgcgc
     7021 gcggcgagac tcaggggaag accatggcag caacacatag gtttcagggt ctactttgag
     7081 tcgcatgtcg ctttcttgaa tacgagcaaa gccttctaca gaagaaccgt cgaaccaagc
     7141 gccatctttc aacacatctt cgagatggcg aatgggcata tccacacttt tgaccgcccc
     7201 gagcacatct gtaaattgga gcgaaagaaa tttgacattg tcttctttca cccgcttgag
     7261 caactctttt gaatccatgt tcatctcctt tttgattttg cttacacagt ttttgatact
     7321 gaccaacagc ttcaagtgta cttcttattc aatttcttct ggaattgcca tttcattttt
     7381 gggagcaggc ttggaatttc gcgcaaggtt gatcatgatg accgcaccga taataattac
     7441 tgatgagacg gcaatacgcg cattcaactc ttcacctgcg aagagccaac caaggagcac
     7501 cgccaccatc gggttgacgt acgcataagt cgaggttaac gatacaggtg cgttttggat
     7561 taaccaaccg taggaaacga acccaactaa ggaaccaaag gtgattagat agatcagacc
     7621 ccaccaagag cgcatcgaca catcgccgaa gttgaatcca cttaattcgc cggttgctac
     7681 gctgacgatg aatagcgacc ctgcacctgc cagcatttgc atacccgtag acatgagcgt
     7741 ggattttggc atatcggcac cgcgtgaata gattgacccc atcgaccaca agataggcgc
     7801 caatacaagc agaattacgc cacttgtttc aaacgtatgt tgtgagcctg caatttctcc
     7861 cggacccacc aatacaaaca caccgccgaa gccaagtaat agacccacaa ttgcctgcca
     7921 ggaaggtttt gtgccaccag agcgcagtga ttcaaaaagc accaaccaaa agggagaggt
     7981 agtaatcata agtgccgcaa tccctgaagg gacgctttgt tcggcaaggc ttacgaatcc
     8041 atttccgccc aagaggagga aggtaccgac aatggcggtg gctttccagt tgcttgtggt
     8101 gggtttgggg tcgcctgcca tatgacgcca cacgaccaga atcgcaccgg aaaccaaaaa
     8161 gcgcagagat gcatgcaaga aaggtggaat ggtttctacg gcaaaacgaa tagctaaata
     8221 ggtagagccc caaacaatcc ataaagcgat taatgcggtc caaagttttg ttttcattct
     8281 tttccttttt gtttaattaa tttactctgt cattacaaat tctcttgcca agatggcttt
     8341 tttgcgagcc gacccataca gataattgcc aaactcgccg ctcttgcgaa tcacccgatg
     8401 gcaggggatg agataggcaa tagggttatc accaacagca ctgcccactg cccgaaccgc
     8461 acgcggattt ccgattgtcg tagcgatgtg ttcgtaggtg gtcaatgcac cggtgggaat
     8521 gctgagcagg gcttcccaca ctttgatctg gaaatttgtg ccgcgtagat gcagcttgag
     8581 aggtgaatca agctgcgccc caagaaagat gcggttaacc aatggcgccg tagcttgata
     8641 atcttcgatc atattggcat ggctccagtg tgccactaga tcgtctattg ctttgccttc
     8701 actggcgttt acaaagctga gattacaaat gccacggtcg gtagtggcga tgagacattt
     8761 gccaaacggc gtgaggtgaa taccgtagtg aatatcgatg cccgctccgc gcgatttgta
     8821 ttcgccggga gtcaccgcct cagtgttgac gaaaagatca tggagccgcc ccaggctgga
     8881 tagtccggtt tgatgcgtgg cttccaaaag attttccgat ttatcgagca attgtttggc
     8941 gtgttccttc gtcagaaatt gcatgaagcg tttgggactg atgcccgccc aacgcgtgaa
     9001 taaccgttga aaatgatatt cactcaagcc aacggcagaa gcgatctctg ccaactcggg
     9061 ttgatgatgt acattggttt caatgtattg aatggcttgt tcgatcagcc gataatgttg
     9121 taaaggttca aggtcttgca gctcacgagt aatggggtcc atatcaatct ccttttcttg
     9181 agggagattg tatgtggcgt tcggcgtgtg ggcgacccgt ttcttgcgca ttttctcatt
     9241 ctactctctt gcgggagtga ttaacaaaaa aacaggcatg ccgggcacgc ctgttttgat
     9301 acacatggta ttaaaacaaa atgcccttcc aaaccaaaac aggtgtacac acctgatcac
     9361 tgcgcttcca ttgtcccgct cgtcgccgtg cggttttctg tttgcgcttg ctttggaagg
     9421 gagaaggaag ccagtgattc tggcttcaga ggtatccgct gatctatccg atttttagac
     9481 ctcaccccta ggtctttagc atcccgttcg cacgggagaa cctccacctc gtgatctcgt
     9541 gtacaacgag acccagccg
//
LOCUS       DAOQFT010000014         9474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3402_length_9474_cov_6.989914, whole genome shotgun sequence.
ACCESSION   DAOQFT010000014 DAOQFT010000000
VERSION     DAOQFT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3402_length_9474_cov_6.989914"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLR93_00660"
     CDS             complement(<1..473)
                     /locus_tag="PLR93_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNH25494.1"
                     /translation="MSRIRLIVILCLTALACQLPSIPILATQTPTQIPTATVTSTPTQ
                     TLTPEPTLTPSITPTPTPLARRVLILSIDGFRPDAIPLAPMPFLTSLLSQSAYSLTAQ
                     TVYPSVTLVSHTSMLSGQCPAKHKVDWNDYIPENGYAQVTDLFDVVHAAGMQTVM"
     gene            515..1255
                     /locus_tag="PLR93_00665"
     CDS             515..1255
                     /locus_tag="PLR93_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNH25495.1"
                     /translation="MDTETSRTLHEELIHHNNVDPHKRGSGLSDFILGAQDGLVNVLG
                     VVLGIAAATNDARVVLVAGLATTFAESISMGAVAFTTTLADADLYQSEREREYRHIAE
                     APMLETKEIRDIYASKGFTGDLLDRIVETITANKDVWVAVMMAEEHQLSPVDRGTAMK
                     AAWVVGLSAIIGSLVPILPFIFLPVSISMWLSVLVTALVLFGIGFYKARVTVGKPMRS
                     GTEMMLIGTISAMAGYLVGVVLKVPPLP"
     gene            complement(1184..1576)
                     /locus_tag="PLR93_00670"
     CDS             complement(1184..1576)
                     /locus_tag="PLR93_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25496.1"
                     /translation="MKLVLIYTLIAGVISAITAPIPGTSLLLTALEVYMIVHLSKSNE
                     SKLGFKEIGYSAMALYGLSTVLKDAALEILTFVPLIGWGAEVIVAMLFVFFLGMLANL
                     YFSKPSRKGRNLENNPDEISSHSTDRPN"
     gene            complement(1573..2061)
                     /locus_tag="PLR93_00675"
     CDS             complement(1573..2061)
                     /locus_tag="PLR93_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25497.1"
                     /translation="MPTINFNGKTYNSIEEMPANERQAFEKLSSLLVDANGNGIPDLL
                     EGDMVQNVMAVHATKTSINVNGQTYHSLEDLPPDLRQSVDGAFQFLSKMGIANTTSDI
                     QTPAMRQEAQPKSKPFLAQESPSAIEEESGRSTFSIVLISIVLCFAVVAATIAIFVFI
                     NR"
     gene            2248..2652
                     /locus_tag="PLR93_00680"
     CDS             2248..2652
                     /locus_tag="PLR93_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNH25498.1"
                     /translation="MPTLKDIEGIGPVNAGKLNKAGVRGLNGLLKLGASRKGRQEIAK
                     ATGINAKVILEWVNRADLFRVKGIGGQYSDLLEAAGVDTAVELSKRQPEALLEAMKKA
                     NAKLNKVNQMPGLSNVKTWIKNAKSLKRVIEY"
     gene            complement(2700..3935)
                     /locus_tag="PLR93_00685"
     CDS             complement(2700..3935)
                     /locus_tag="PLR93_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNH25499.1"
                     /translation="MQTPWEYRFAHRTQRMGSSVIRELLKFTEQPDIISFAGGLPAPE
                     VFPVNEFQEACNRVLTEHGAQALQYSTTEGYRPLREMISRHTDRYKVSVTPENILITS
                     GSQQALDFLGRLFINRGDYIVVESPTYLGALQAWNAYGAQYISVPADENGMIVEELEK
                     ALRVGPKFIYVLPNFQNPSGSTLSLERRKRLVALADQYGVPIIEDDPYGQLRYDGEHI
                     PSVVYLDAQYRNDGDHEYTGNVIYLSTFSKLLAPGLRLAWVVAPPKVIQKLVMTKQAA
                     DLHTSSFNQQVAYEVGKGGFLDEHVKVIRATYKERRDVMLEMMDEMFPAEMRWTKPQG
                     GMFLWSMMPDGVDAADVLRIAIERKVAFVPGAAFHPNGGGDNTMRINFSYSSPEMIRE
                     GITRLGSTLKEVLKKNGQH"
     gene            complement(4376..5233)
                     /locus_tag="PLR93_00690"
     CDS             complement(4376..5233)
                     /locus_tag="PLR93_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH25500.1"
                     /translation="MPILALVLLFLSASMHALWNFLLKSAEEKYVAMGWQTILSGSLA
                     LLVMVFYSGLPPKEMWLFAVISMLLEAIYFILLCIAYSDHDFSLVYPIARGAAPALLV
                     LWTAIFLHEQLTTGGYIGLAMITLGMMVIGGTTLLQSKGEKVHLKGILTALSVALVIS
                     LYTFIDGKAVKSGPALPYGLSMFVMVPFVVTPYIANQYGWDSFGRVWQKNRNYLLIGG
                     VLGLIAYMLALFAYTFAPLSYSGAIREVSAVIGAFLGWRFLKEQMGGVRVLGSAIVFI
                     GVMVIAIFG"
     gene            complement(5238..6128)
                     /locus_tag="PLR93_00695"
     CDS             complement(5238..6128)
                     /locus_tag="PLR93_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH25501.1"
                     /translation="MAFSVILAPMPSEWIETNQIKLHVVTDGPTDGTPVILLHGFPEF
                     HYGWRKQVPALTEAGFYVITPDQRGYNLSEKPKGISSYKLESLAKDIIGLFDHYGIQK
                     ARLVGHDWGAVVAWYVGIRYPERLEKLAILNVPHPDVMTHFLLHDSEQRKKSWYVYFF
                     QLPFITEWDLSRQNYRNMARMLVGSGLKSTFSRRDIEEYKKAWSQPGALSAMINWYRS
                     IVRESLKDLFRKRQPPRRIHVQTLMMWGMKDVALSHKMVQPSIDLCDDGKLIFFEHAT
                     HWVQHDEANEVNKNLIEFLR"
     gene            complement(6129..>9474)
                     /locus_tag="PLR93_00700"
     CDS             complement(6129..>9474)
                     /locus_tag="PLR93_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH25502.1"
                     /translation="LTVYIWMFMSIMTAGCILLIVMDPSQLRRPLLIIIILNSLYSLF
                     YYIMKTGKVQLASFLTIASTWLMLTVVVVTGGGVHSPAFSAYLMLTFLAGITLGVKPM
                     MWTAIASLIVAGGMEYLELTGLLPEPRYQTSHFSFVLLQGIMLFMFVALQFYASSLIK
                     NSFQKAQDELRERKIIEQALSTSQRRFIAIFENSPIPTAITTLNDLKLIDANAMFIQL
                     YGYSLDELHGEQTRILGSLENQAQMLLLLNKNGFVRDFETSLRRKDTETRYVSVTIGK
                     LNIDASDCLIFQVLDVTEKHNLAQSLVESNQRFAQLTRHIHEVFWVSEEVGKLEYVSP
                     AFEYIFGFPAEDLYQGKDYIDLVHPEDRQIVLDSFEYDMRGENSLFEYRIIRPDGEIR
                     WVSDRSYPILGEHGELIRTVGITTDITEQKHSEAERRELTSILEDAEQQVGLASWSQD
                     ILTGKSKWSPYMFTLFGFDPAEGIPSMPDYLERIHPEDRELLGSALFKMMQGEETSEM
                     FTYRTNPHYGEMRFLKPTYRTEKDANGRVIKFLGTVLDITEQKRSATELERRQKLLET
                     VILAGKNITSIIDFDKCLQEIHRNIISNLGFERVGLFIYDPKRHYVQGTYGTDRNGNP
                     VSNDWYYEQINENSEWQVALESPTGIAVIDDYGLVRNPPPDNEMYGVKQHITLAAWSG
                     DTPVALISVDNLLTQKPITPADIEALQLFAGYAGLAIENARLHTGLENLIEKRTDELT
                     QSQKSLQLFLDTANDLIQSLDESGRYIYVNQSWCRTLGFTLEEASKLDMLQVVDKEYQ
                     DHCLNIFRNMMADGVSQMIEVGFRTKQGHLVIVEGSISIQTRSDGKRVTNGFFRDVTE
                     RKQAEIAMRHANLEMEKALRMKDEFMANMSHELRTPLNAILGLSESLLEETVGSINSR
                     QHKYLNTISESGRHLLELINDILYLAKIESGQIHLASNSVNIKTLCEASLRIVDQLAK
                     SKKQVMSLEVADEIKFVQADERRLKQMVVNLLSNAIKFTPENGQVGIRALIDASRTFL
                     NIEVWDTGIGIKADDLSHIFQPFIQLNSGLARESSGTGLGLALVAQMARLHGGSITVR
                     SEIGKGSSFILSLPWTPELSDAPQEQ"
BASE COUNT         2409 a         2174 c         2326 g         2565 t
ORIGIN      
        1 tacattacgg tttgcatacc ggcggcatgg acgacatcga aaaggtcagt cacttgagcg
       61 tagccatttt cagggatgta gtcgttccaa tccactttgt gcttggcggg gcattgtccg
      121 ctgagcatgg aggtgtgtga aacgagagta acgctgggat atacggtttg tgccgtaagt
      181 gaataggctg attgacttag caacgaagtg aggaatggca tcggcgccag tggaatggca
      241 tcggggcgaa acccgtcgat ggagaggatc agtacgcggc gagcgagagg cgtgggggtg
      301 ggtgtaatcg agggcgtcag cgttggctct ggcgtcagcg tttgcgtcgg ggttgatgtg
      361 accgttgcag taggaatttg ggttggcgtt tgtgtggcaa ggatgggaat ggatgggagt
      421 tgacaggcaa gcgccgtaag acaaagaata acaataagtc ggatgcgtga catttgtcca
      481 ttataaataa ctttcagcgt agtatgattt gaatatggat actgaaacct ctcgaacttt
      541 gcatgaagaa ttgattcatc ataacaacgt ggacccacac aaacgtggct cgggtctttc
      601 ggactttatt ttgggtgcgc aagacggctt ggtcaacgtc ctcggcgtgg tgctgggaat
      661 cgctgccgcg acgaacgatg cacgcgtggt gttggtcgca ggcttggcaa ccacctttgc
      721 ggaatccatt tcgatggggg cagtggcttt caccaccact ctcgctgatg cggacctata
      781 tcaaagcgag cgtgaacgcg aatatcgaca tattgccgaa gcgcccatgc ttgagacaaa
      841 agagatccgc gatatttatg ccagcaaagg ctttacaggg gacttgttag accgcatcgt
      901 ggaaacaatc actgcgaaca aagatgtgtg ggtggcggtg atgatggcag aagaacatca
      961 actctcccct gtagatcgtg gcactgccat gaaggctgct tgggtggttg ggctttcggc
     1021 aatcattggt tcgttggtgc cgatcttgcc gttcatcttc ttaccagttt cgatcagtat
     1081 gtggctctcg gtattggtga cggcgttggt cttgtttgga atcggctttt ataaagcgcg
     1141 cgtaaccgta ggcaaaccca tgcgcagcgg cacagagatg atgctaattg ggacgatcag
     1201 tgctatggct ggatatctcg tcggggttgt tctcaaggtt ccgccccttc cgtgatggtt
     1261 tgctgaaata taaatttgcc agcatgccaa ggaagaagac aaagagcatc gctacgatca
     1321 cttctgcacc ccagccaatg agcggaacga aagtgagaat ttcaagcgcg gcgtctttta
     1381 acacggtgga aagtccgtac agtgccatgg ctgaatagcc aatttccttg aaccctaatt
     1441 tggattcatt gctttttgag aggtgtacga tcatgtacac ctctaatgct gttaatagca
     1501 gggatgtgcc agggattggc gcagtgatgg cggagatcac gccggcgata agggtgtaaa
     1561 tgaggacgag tttcatcggt tgataaagac gaaaatagca atcgtggcgg cgacaacagc
     1621 gaagcaaagc acaatgctga tcaaaacgat tgagaaggtg ctacgtccgc tttcttcttc
     1681 aatcgcggag ggagattctt gcgccagaaa cggcttggac tttggttgcg cttcctgacg
     1741 cattgccgga gtttgaatgt ctgacgtcgt gtttgcgata cccattttcg ataagaattg
     1801 aaaagcgcca tctacacttt gacgcaaatc tggtggcaag tcttcaagag agtgataggt
     1861 ttgtccgttg acattgatgc tcgtttttgt ggcgtgaact gccatcacat tctgcaccat
     1921 atcgccttca agcaggtcgg gaatgccatt gccgttcgca tccacaagta agctggataa
     1981 tttttcaaaa gcctgtcttt cattggcggg catttcttct atgctattgt aggtctttcc
     2041 attgaaatta atagtgggca tcatgaactc ctgcgtcttg tctctccatt ttacacgcag
     2101 atattaccaa acctaaaagt aaagtttgta tcgtcagttt ttagataatg tggaaagaac
     2161 aataaaatct tttgtgctta agaatggata aagaattggt tttttagtaa ttagatgtta
     2221 tagtattgaa taaggagata acaacatatg cctacactaa aagatattga aggaattggt
     2281 cccgtcaatg ctggcaaact taacaaggct ggtgttcggg gcttgaacgg gctcttgaaa
     2341 ttgggagctt ctcgcaaggg acgccaggaa attgcaaaag ctactggtat taatgcgaaa
     2401 gttatccttg aatgggttaa ccgtgcagac ctgtttcgtg taaagggaat tggtggtcaa
     2461 tattccgatt tgctcgaagc tgctggtgtg gatactgcgg ttgaactttc caaacgccag
     2521 ccagaagcct tgcttgaggc aatgaagaag gcaaatgcta aactgaacaa ggtcaatcaa
     2581 atgcccggct tgagcaatgt caaaacttgg atcaagaacg cgaaatcact caaacgtgtg
     2641 attgagtact aaataaaaaa atcccgatgt gatgaacaat cgggattttt ttatttaact
     2701 tagtgttgtc cgttcttctt gagaacttct ttcaacgtag agcccagcct tgtaatccct
     2761 tcgcggatca tctctggtga cgaataagag aagttgattc gcatggtgtt gtctccaccg
     2821 ccgttagggt gaaaggcagc gcccggcaca aatgccactt tgcgttcaat ggcaattcgc
     2881 aatacgtctg cggcgtccac accatcgggc atcatgctcc ataagaacat gccgccttga
     2941 ggttttgtcc agcgcatttc ggctgggaac atttcatcca tcatttcaag catcacatcg
     3001 cggcgctctt tgtacgtggc gcgaatcacc tttacatgtt catccaagaa tccgcctttg
     3061 cccacttcgt atgccacttg ttgattaaaa ctggacgtat gtaggtctgc ggcttgtttg
     3121 gtcattacca atttttggat gaccttgggt ggtgccacta cccaggcaag cctcaaaccg
     3181 ggggcaagta actttgagaa cgtactcaag taaatgacat tgcctgtgta ttcatggtcg
     3241 ccatcattgc gatattgcgc atccagataa acaaccgaag gaatatgctc gccatcgtag
     3301 cgtagttgac catagggatc atcttcaatg atgggcacac catattgatc ggcaagggca
     3361 accaaacgtt ttctgcgttc gaggctgaga gtagagccag aagggttctg aaagtttgga
     3421 agcacgtaaa tgaatttcgg tccgacccga agcgcttttt ccaattcctc tacgatcatg
     3481 ccgttctcat ccgcaggtac cgaaatgtat tgtgcgccat aggcattcca agcttgtaac
     3541 gcgccgagat acgtaggcga ctccaccaca atgtaatccc cccgattgat gaacaacctc
     3601 ccaagaaaat ccaacgcttg ttgcgaacca gaagtgatca aaatattttc aggtgtgact
     3661 gaaactttat aacggtccgt atggcgagag atcatctcgc gcaatgggcg atacccctct
     3721 gtggtgctgt attgcaatgc ttgtgcgccg tgttcggtca acacacgatt gcaggcttct
     3781 tgaaattcat tcaccgggaa aacctcaggg gcgggcaaac cgccggcaaa cgaaatgata
     3841 tcgggttgct ctgtgaactt caacaattca cgaataaccg agcttcccat tctctgtgta
     3901 cggtgggcga agcgatactc ccagggtgtc tgcattctta ctccttagtg tagagataaa
     3961 aaaagcctga caggtacgcg ggtacccatc aggcttgctg actcatggca atcgaatgcc
     4021 gaaacttaat atagccagaa atccattcat ggcaccttta tcttacctat ttttggcaga
     4081 ggacgcaact gttttttgta actgtcgata gcttgaaaag acgtgacaaa ttgcaggatg
     4141 ctccgcctct tcacctgttt ttgcctgttt ctgtccattg gaatctatct acactgtgca
     4201 caacttctct tatcaaaccc tcagaaattt cgtagttttc aacccttgcg cgtttttgcc
     4261 attttggtaa aataggtctt gcaacgtcca gggtgccccc ctcaccctgg acgttgcact
     4321 ttaatttcca aacaaaaagc cgtcaatgta ggcggctttt ttcatttctc tctgtttagc
     4381 caaagattgc aatcaccatc acccctataa acacaatcgc agacccaagc actcgcaccc
     4441 cgcccatttg ctctttcaag aagcgccagc ccaaaaacgc tccaatcact gcactcacct
     4501 cgcgaatcgc acctgaataa ctaagcggtg caaaggtgta ggcaaataaa gccaacatat
     4561 aggcaattaa tcccaacacg ccgccaatca gcagataatt gcgatttttt tgccacaccc
     4621 tgccaaatga atcccagcca tattggttgg caatatacgg tgtgactacg aacggcacca
     4681 tcacaaacat cgacaaacca tatggtagtg caggtccgct tttgacggct ttgccatcaa
     4741 tgaaagtata gagcgaaatg accaacgcca cgctcaacgc ggtaagaatg cctttgaggt
     4801 gcaccttctc acctttgctt tggagcagag tcgtcccgcc gatcaccatc atgccaagcg
     4861 taatcattgc cagaccaata tagccgccgg tggtcaattg ttcatgtaag aagatagctg
     4921 tccacaatac tagtagggca ggtgcggctc cgcgggcaat ggggtagacc agcgaaaaat
     4981 catggtcact gtatgcgatg caaagcaaaa tgaaataaat ggcttccaaa agcatgctga
     5041 taacggcaaa taaccacatc tctttgggcg gcaagcctga atagaagacc atcacaagca
     5101 aagccagtga cccgcttaag atggtttgcc accccatggc aacatatttt tcttctgcgc
     5161 ttttgagtag aaaattccac agcgcatgca tggatgcaga cagaaacaga agaacaagcg
     5221 caagaatggg cattccgtta cctcaggaac tcgatcagat ttttattcac ttcattggct
     5281 tcatcgtgtt gaacccaatg cgtggcatgt tcgaaaaaaa tcaacttgcc gtcatcacac
     5341 aggtcaatgg acggttgcac cattttatgg ctgagagcta cgtctttcat gccccacatc
     5401 atcaaagtct gcacgtggat gcggcgtgga ggctgccttt tgcggaataa gtctttcagg
     5461 ctttctctca caatcgaacg ataccagttg atcatggcag ataaggcacc gggctgtgac
     5521 catgcttttt tgtattcttc gatatcgcgg cggctaaatg tgctcttgag acctgaccca
     5581 accaacatgc gcgccatgtt gcgataattc tgacggctta aatcccactc ggtgatgaaa
     5641 ggcagttgaa agaaatacac ataccaagac tttttcctct gctcagagtc atgcaacaag
     5701 aaatgtgtca ttacatcagg gtgtggcaca ttgaggatag cgagcttttc caagcgttct
     5761 ggatagcgaa tgccaacata ccaagccacc accgcacccc agtcgtgccc aacgaggcgg
     5821 gctttttgaa tcccataatg atcaaagaga ccgatgatgt cttttgccag agactccaac
     5881 ttgtaggaag aaatcccttt gggtttctct gagaggttgt acccgcgctg atcgggcgtg
     5941 atgacgtaaa agcccgcttc cgtcaacgct gggacttgct tgcgccaccc ataatgaaac
     6001 tcagggaagc catgcaataa aatgaccggc gtcccgtctg tgggtccgtc ggtcacgaca
     6061 tgaagtttta tttgattggt ttcaatccat tcgcttggca ttggcgcgag tataacggaa
     6121 aatgccaatt attgctcctg aggggcatct gacagttctg gcgtccatgg caagcttagg
     6181 atgaaactgc tgccttttcc aatttcgctg cgaactgtaa tactgccccc gtggaggcga
     6241 gccatttgtg ccaccaatgc caaccccagc cctgtgcccg atgactcacg cgccaaacca
     6301 gaatttaatt gaatgaaggg ttggaagatg tgtgagaggt catcagcttt gatgccgatg
     6361 cctgtatccc agacttcaat gtttaaaaag gtgcgtgacg catcgatcaa tgcccgtatc
     6421 cctacttgac cgttctcggg cgtaaattta atcgcattgc taagcaagtt gacaaccatt
     6481 tgttttaggc gacgttcatc tgcttggaca aacttaatct catcggcgac ttcgagactc
     6541 atgacttgtt ttttgctttt tgccagttgg tcaacgatgc ggagagaggc ttcgcacaag
     6601 gtcttgatgt ttacagagtt ggaggctaag tggatttgcc ctgattcgat ctttgccaga
     6661 tacagaatgt cattgatcaa ttccagtaga tgacgtccgc tttcgctaat ggtgttgaga
     6721 tatttatgtt ggcgggaatt gatggagccg acagtttctt cgagcaggct ttctgaaagt
     6781 cctaaaattg cattgagcgg ggttcgaagt tcatggctca tatttgccat gaactcatct
     6841 ttcatgcgca atgctttttc catttcgagg ttggcgtgtc gcatggcgat ttcggcttgt
     6901 ttgcgttcgg tcacatcacg gaagaaacca ttggtgaccc gtttcccgtc gcttcgtgtt
     6961 tgaatgctga tactgccttc cacgatcaca agatgacctt gcttggtgcg aaagccgact
     7021 tcaatcatct gtgacacccc atctgccatc atatttctaa aaatgttcaa acagtggtct
     7081 tgatattctt tgtccactac ttggagcata tctaatttgc tggcttcttc aagggtgaat
     7141 cctaatgttc gacaccagga ttgattgaca tagatatagc gtccgctttc atcgaggctt
     7201 tgaattaggt cattggcggt gtctaaaaac agttgcagac ttttttggct ttgcgttaat
     7261 tcatcggttc gtttttctat caggttttca agcccggtat gtaggcgagc gttttcgatg
     7321 gcaagcccgg cataccctgc aaacaattgc aaggcttcaa tatccgccgg ggtaataggc
     7381 ttttgggtca ataagttatc gacagagatc aaagccactg gcgtatctcc agaccaggcg
     7441 gctagagtga tgtgctgctt aactccatac atctcattat ctggtggtgg gttgcggact
     7501 agcccgtaat cgtcgatcac ggcaatgcct gttgggcttt ccaaagcgac ctgccattcg
     7561 ctattttcgt ttatttgttc gtaataccaa tcattactga ctggattgcc gttgcgatct
     7621 gtgccgtaag tgccttgtac ataatgtctt tttgggtcat agatgaataa gcctacacgt
     7681 tcgaacccga ggttgctaat aatgtttcgg tgaatctcct gcaagcattt gtcgaaatca
     7741 ataatggagg tgatgttctt tccggcaaga atgacggttt caagtagttt ttgtcttcgt
     7801 tccaattctg ttgcactgcg cttttgctct gtaatatcca gcacggtgcc aaggaatttt
     7861 atgacgcgcc cattcgcatc tttttcggtg cgataggtgg gtttcaagaa ccgcatttct
     7921 ccatagtgtg gattggtgcg gtaggtaaac atttcggacg tttcttcgcc ttgcatcatc
     7981 ttgaataaag cggagccaag cagttctcga tcttcggggt ggatgcgttc caaataatct
     8041 ggcatgcttg gaatgccctc tgccgggtca aacccgaaca gtgtgaacat ataggggctc
     8101 cacttgcttt ttcctgtcag aatatcttga gaccaacttg ccagcccaac ttgttgttca
     8161 gcatcttcca gaatggatgt tagttcccgg cgctcggctt ctgagtgttt ttgttctgtg
     8221 atatcggtcg tgattcctac tgtgcgaatg agttcgccat gttccccaag gatcgggtag
     8281 gaacggtctg agacccagcg aatttcaccg tctgggcgta tgatgcggta ttcgaatagc
     8341 gagttctcgc cacgcatatc atactcaaat gagtcgagta cgatctggcg gtcttccggg
     8401 tgtaccaagt cgatgtaatc ttttccttgg tacaagtctt cggctgggaa cccaaaaatg
     8461 tattcgaacg ccgggctgac gtattccagt ttgcctactt cttcagagac ccaaaacact
     8521 tcgtggatgt gccttgtaag ttgggcaaaa cgttgattgc tttcgaccag agattgcgct
     8581 agattgtgtt tttcggtgac atccaatacc tgaaagatca ggcagtctga tgcatcaata
     8641 ttcagctttc cgatggttac cgacacatag cgggtctcag tatcttttcg gcggagcgac
     8701 gtttcaaagt ctcgtacaaa gccgttcttg ttcaagagca aaagcatctg cgcttgattt
     8761 tcaaggctac ccaggattct tgtttgttcg ccatgcaatt catcgagaga atagccatat
     8821 aactgaataa acatggcgtt agcatcgatc agtttgaggt cgttcaatgt tgtgatggca
     8881 gtgggaatgg gactgttctc gaatatggca atgaagcgtc tctggctggt ggaaagcgcc
     8941 tgttctatta ttttgcgttc tcttaattca tcttgagctt tttgaaaaga attcttaata
     9001 agtgaactgg catagaattg caaggccaca aacataaata gcataatgcc ttgcagtaac
     9061 acaaaactaa aatgggaggt ttgataccgc ggctctggta aaagaccggt caattcgaga
     9121 tattccattc cgcctgcaac gatgagactt gcaatagcag tccacatcat gggcttgact
     9181 cccagggtaa tgcctgcgag aaaggttagc atgaggtaag cactaaatgc cggcgaatgg
     9241 acgccgccac cggttactac aaccacagta agcatcagcc atgtcgatgc aatcgtgaga
     9301 aaactggcta gctgcacctt gccggtcttc atgatgtaat aaaacagcga atataaggaa
     9361 ttgagaatga taatgatgag aagcggacgt cttagctgag acgggtccat aacgatcaac
     9421 aggatgcatc ctgccgtcat gatgctcatg aacatccaaa tatagacggt gagc
//
LOCUS       DAOQFT010000015         9302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3491_length_9302_cov_7.759922, whole genome shotgun sequence.
ACCESSION   DAOQFT010000015 DAOQFT010000000
VERSION     DAOQFT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3491_length_9302_cov_7.759922"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLR93_00705"
     CDS             complement(<1..858)
                     /locus_tag="PLR93_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNH25503.1"
                     /translation="MKSHITLLLVFLIVGMLVLNACGAPAATEQYYSEEMAAATEAAA
                     TEAAFIAEEAPAAPQAEADSEASLKNGSVYETGSADNVAVTTHLIIKNADMRLLVEDS
                     DQAIDRITQVVGDSGGYIISSRVWNQTYLDGKNYKYATITMGVPVQTFERALNRLRDL
                     SLEVLDENASGEDVTDQYVDLQSQLVNLEATRERIKSFLDQAKTVEEALLINQQLSEI
                     ERQIEEIKGRINFLQDRSSFSTITVNIEPKLPDYVPPTPTATPSPTPTPKTPWKPGET
                     AEKAIGTVTW"
     gene            complement(1090..1527)
                     /locus_tag="PLR93_00710"
     CDS             complement(1090..1527)
                     /locus_tag="PLR93_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH25504.1"
                     /translation="MPKRSGIILIHEGKLALIERYKKGRHYFAFPGGGVDEGETDEQA
                     AIREAEEELGIRVKIVQKAATVLRTGRRDQVYFVVNWVDGEFGTGTGEEYTEPYEENG
                     TYAPLWMPLEDVLVKNVVPRGLAELTVRSHQEGWKENVIIHES"
     gene            complement(1570..2496)
                     /locus_tag="PLR93_00715"
     CDS             complement(1570..2496)
                     /locus_tag="PLR93_00715"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNH25505.1"
                     /translation="MQHYRSLEEVSLKNSYLTVGVFDGVHRGHREIIQKLVKDAHANN
                     APAVVLTFSPHPATVLTGREIHCLTTPDERADLLGSLGVDAVITQRFTRDLSTVPAHE
                     YMSSLKTHLGLSHLLIGYDFALGKGREGNAPRLAEIGTELGYTVEVVPALGDESGVIS
                     STEIRKLVSIGNMPEASKLLGYPYRMSGEVIHGAGRGKQINFPTANVDYAPQKASPPN
                     GIYACWAKLGDERFMAATNIGFNPTFTPERKTQSLEAYLLDFDRDIYGDVLTLEFVAR
                     LREEIRYTNVEALIEQIHDDVLQTRKLLSDLK"
     gene            complement(2543..3472)
                     /gene="truB"
                     /locus_tag="PLR93_00720"
     CDS             complement(2543..3472)
                     /gene="truB"
                     /locus_tag="PLR93_00720"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNH25506.1"
                     /translation="MNSQDVKNAISGALVIDKPVGMTSHDVVQAIRNGTGLRRAGHTG
                     TLDPRASGVLVILVGPAVRLSEYVSASDKRYQAIIRLGGTTDTYDAEGKFTPTKDPSN
                     ITEAEFEEALKTFVGEIEQTPPPYSAVKVQGRKAYEMARKGEEVELEPRTITVHHLEV
                     LEWTPPEVVIDVHCSSGTYVRSLANDLGKKLGCGAYLVGLRRTKSGKFTLRDAVPLRK
                     LQEAFNAGNWYQYLIPAAEALGDWPAIELNPDEVEAVRHGHRVVAKASDIVHEKVRGV
                     STQGELVALMVQADNVETGNKEWQPKKVFFTSD"
     gene            complement(3564..4520)
                     /locus_tag="PLR93_00725"
     CDS             complement(3564..4520)
                     /locus_tag="PLR93_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNH25507.1"
                     /translation="MTDQISGDIKNRLADAKKVVIASHVRPDGDAIGSLLGLGLALIE
                     AGKTVEMILVDGAPASFKFLEGSELIKKEPTGGHDTFITVDCADFKRTGKIFESFGQP
                     DINIDHHKTNEKFGKLNLIEAEEVATAAILTNHLPTWGLKITKPVAAALLTGILTDTL
                     GFRTSNVTPEALRQAATLMESGVDLPEIYMQSLVRKSFPAAKYWGAGLSSLESKDGIV
                     WGTLTLEDRKSASYGGNDDADLINMISAIEGHKVGMVFVQQTDNKVKISWRALQAGID
                     VSPIAKHFNGGGHAAAAGADIEGSLADIKKDALNVTKKLLNL"
     gene            complement(4560..4910)
                     /gene="rbfA"
                     /locus_tag="PLR93_00730"
     CDS             complement(4560..4910)
                     /gene="rbfA"
                     /locus_tag="PLR93_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNH25508.1"
                     /translation="MPSGIRIKRIEDRIQQELSEMLIHEISDPRLKQIYVTGIKVDRE
                     LAFAEVYVSAVEGASRSEEVLAGLESASGFLRRTLSARVELRSFPKLRFHWDPTPENA
                     DHIEKLLAGLRNKK"
     gene            complement(4975..6744)
                     /gene="infB"
                     /locus_tag="PLR93_00735"
     CDS             complement(4975..6744)
                     /gene="infB"
                     /locus_tag="PLR93_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNH25509.1"
                     /translation="MSENGNKIELPASIVVRDLAQKIEKSPIDLIKKLMTNGVMATIN
                     QTVDYDTAAIVVAEYGFEAVPEVVEEESKEEAGEVPMWRQMIAGEDKAQLKQRPPVVT
                     ILGHVDHGKTSLLDAIRSTDVAAGEAGGITQHIGAYQVEMKGRLITFLDTPGHAAFTQ
                     MRARGAQGADIVILVVAADDGVMPQTKEAIAHAKAARVPIVVALNKVDKPNANPDRVK
                     QQLAELELVPDDWGGNTMVVPVSAKQKKGIDDLLEGVLLVADSNEIKANPSGKVIGTV
                     IEAELDKSKGVLATLLVQNGTLESGDVVVAGTAHGKLRAITDYKGKPVKKAGPSTPVA
                     VMGLSDVPSAGDLFEVVPSEKEARAIVAERMEVLKAQAQSRKKLSLEDLFSKVQAGEA
                     KELNLIVKADVQGSLDPIITELNKLGQGEIGLKVLHAETGNIGNNDVMLASASKAIMI
                     GFNVQADVDARRMAEKEGVDIRLYEIIYRMTEDIEKALNGMLEPDLKEKVLGKATVLQ
                     VFTASKFGRVAGCRVTDGELKRGAKVRLYRDSDIIYEGDLSSLRHEKDDVKEIRQGFE
                     CGVGFKNFSDIQSGDTLVCYILE"
     gene            complement(6829..7128)
                     /locus_tag="PLR93_00740"
     CDS             complement(6829..7128)
                     /locus_tag="PLR93_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNH25510.1"
                     /translation="MVKKPVQRVKHIPQRTCVGCREILSKRQLLRIVRTAEGVRVDPT
                     SKLAGRGAYLHDKRSCWARGLKGGLAHALKTEISAEDRARLEEFMNTLPEAETDG"
     gene            complement(7173..9047)
                     /gene="nusA"
                     /locus_tag="PLR93_00745"
     CDS             complement(7173..9047)
                     /gene="nusA"
                     /locus_tag="PLR93_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNH25511.1"
                     /translation="MAKNDFALAFNEVLEEKQLAKDVVITAIESAMVSAYRRAVGAST
                     AQYVESKLDPETGIVTVYAEKEVVEDSIVDDRTEVLLEVARKVNPECQPGDMVVVEST
                     PADFGRVAAQTARQVIQQRIREAERSNQMEYYQRQEGEIVSGLVQAINAQGITIGLDM
                     KAEGVMPNNQRIPGEKLKLHDRVRAVVMEVKDGTRGPQIILSRAHRNFLRRLMENEVP
                     EIYHGIVEIRAIAREPGARAKVAVSATQPGIDPVGACVGQKGVRIQAIVKELHDEKID
                     IILWDADPVAYISKAISPAKVTGVYLTEEERTATVVVGEDQLSLAIGRDGQNARLAAK
                     LTSWRIDIKSLSEAAGDSLAKLQGDAELQNMLKAAVEVMPQVEEILAKKAENRLITPE
                     EFSTLEQFVDRVERRMILAKEEASREEDERIAAARAEIPAAAFNMPLDQATNLKEHVF
                     NILTEAGYETVGQLIFDMKTDQNKVLGLAGIGGKAITNIEETLAALTFPEPVAEPKVE
                     ETPAVVAEPVVTETAPVVETPVVEKPAEKKKEPKRVIEEEEEDGEVSKDGVNLDELFQ
                     MKPEMFQAGEGADEESDDKKKGKKVKKKGVALEFDENLGEVVSRKKHKRSDGGDDEAW
                     "
BASE COUNT         2078 a         2400 c         2257 g         2567 t
ORIGIN      
        1 ccatgtgacc gttccaatcg ctttttcagc tgtttcaccg ggtttccatg gggtcttcgg
       61 tgtgggggta gggctgggag ttgctgtagg cgtaggcgga acataatctg gcagtttcgg
      121 ctcaatgttt accgtaatcg ttgagaagga agaccggtct tgcaggaaat tgatgcgtcc
      181 tttgatctct tcgatttgac gttcaatttc agagagctgc tggttgatga gcagagcttc
      241 ttctacagtt ttggcttgat cgagaaaaga tttgatgcgt tcacgggtgg cttcgaggtt
      301 gacgagttga gattgtaaat ctacatactg gtcagtaaca tcttcaccag aggcattttc
      361 gtccaagact tccagtgaaa ggtcgcgtag gcggttgaga gctcgctcaa acgtctgaac
      421 ggggacgccc atggtaatgg tggcatattt gtaatttttg ccatccaaat aggtctgatt
      481 ccacactctt gaactgatga tatacccacc cgagtcacca acgacctgtg taatgcggtc
      541 gatagcttga tctgaatctt cgaccaacaa gcgcatatcg gcatttttga tgatgaggtg
      601 cgtagtgact gccacattat cagccgaacc ggtctcatat actgacccat ttttcaagct
      661 ggcttcagag tcagcttcgg cttgaggtgc agctggcgct tcttcggcaa tgaatgcagc
      721 ttctgtcgct gcggcttctg ttgcagctgc catttcttcc gaatagtatt gttcggttgc
      781 ggcgggagct ccgcaagcgt ttaggaccag cattcccaca ataaggaaca ccagcagcaa
      841 tgtgatgtgt gatttcattt catcctccgt atttaatttt tgaatttcgg tgttgtacaa
      901 gtgacgtacc tgtggtggaa aaagttccta ggtcatggga tttcctcctt tcatttcttt
      961 atacagattc ggcgtatcca tataaaaagg ggtttctttc atcatacaaa aaataaacct
     1021 gccacttcta atacttaaga gtggattttg aactgaaaat aaaaaacgcc gcttttgcgg
     1081 cgttttgact taagactcgt ggatgattac gttttctttc caaccctctt ggtgagatcg
     1141 aaccgttagc tcggcaagcc cacgcggcac aacgtttttg accagcacat cttccaaagg
     1201 catccaaagc ggggcatacg tcccattttc ctcatagggt tccgtatatt cttcacccgt
     1261 tcccgtgcca aattccccat ccacccagtt caccacgaaa tacacctggt cgcgccgccc
     1321 tgtgcgtaag accgtagcag ccttttgcac gatcttcacc cgaatgccta attcctcttc
     1381 cgcttcacga atggcagctt gttcgtcagt ttcgccctcg tctacgccgc cgccgggaaa
     1441 agcaaaataa tgccgcccct ttttgtagcg ttcgatcagt gccagctttc cttcatgaat
     1501 caaaataatt cctgaacgtt taggcatatc actcctttga aacgcaaaaa atatcgggca
     1561 attgcccgat tatttcaaat cactcaatag cttacgggtc tgcagcacat catcatgaat
     1621 ctgttcgatc agcgcttcta cgtttgtgta acgtatttct tcccgtagcc gggcaacaaa
     1681 ttccagagtc aatacgtcac cataaatatc acggtcaaaa tcgagtaaat aggcttccaa
     1741 gctctgagtt tttcgctcag gggtaaatgt agggttgaag ccaatatttg ttgctgccat
     1801 aaaacgttca tcgcccagtt ttgcccaaca agcataaata ccattgggcg gagacgcttt
     1861 ctggggagca taatcgacat tcgcagtagg gaagttgatt tgctttccgc gcccagcccc
     1921 gtgaatgact tctccgctca tgcggtaggg atacccaagc agtttggatg cttcgggcat
     1981 attgccgatg gaaaccagct tgcgaatctc ggtggatgag atcacgccgc tttcgtcgcc
     2041 caaagctggt acaacttcga cggtgtatcc taactccgtg ccaatctcgg cgaggcgtgg
     2101 tgcattgcct tcacgtcctt tcccgagggc aaaatcataa ccaattagca gatggctaag
     2161 cccaaggtga gttttcagac tggacatata ttcatgggca gggacagttg aaaggtcccg
     2221 tgtgaagcgt tgggtgatga ctgcatccac acccaaggaa ccgagcaaat ccgcccgttc
     2281 atcgggtgta gtcaggcaat gaatttctct gcctgtgagt accgtggctg ggtgcggaga
     2341 aaaggtcagc actacagcag gggcgttgtt ggcgtgtgca tctttcacta gtttttggat
     2401 gatttcacgg tgcccacgat gtacaccatc gaatacaccc acagtaagat atgaattttt
     2461 gagagagact tcttcaagag agcggtaatg ttgcatatga ataaagccgc ctttgacggc
     2521 ggctttattt tatcattccg tgttaatcag acgtgaagaa cactttttta ggttgccatt
     2581 ctttgttgcc ggtttcgaca ttatctgcct gaaccatgag tgcaaccaat tcgccttggg
     2641 tgctgacgcc gcgcactttt tcgtgtacga tgtctgaggc ttttgctacc acgcgatgac
     2701 catgacgtac ggcttccact tcgtcggggt tgagctcaat agcgggccaa tcgccgagtg
     2761 cttcagccgc agggatgagg tattgatacc aattgcctgc attgaaggct tcttgcagtt
     2821 tgcgcagcgg aacggcatcg cgcagggtga actttccgct cttggtgcgg cgtaagccaa
     2881 cgagataagc gccacagcct aattttttac cgaggtcatt cgccagcgaa cggacatacg
     2941 ttccggacga gcaatgaaca tcgatcacca cttcgggggg agtccattcg agcacttcga
     3001 ggtggtgaac ggtaatggtg cgaggttcga gttctacttc ttcgcctttg cgcgccattt
     3061 cgtaggcttt gcgtccctgt accttaacgg cagaataggg aggcggggtt tgctcgattt
     3121 cacccacaaa tgttttcaat gcttcttcaa attccgcttc ggtgatattt gaagggtctt
     3181 tggtgggggt gaactttcct tcggcatcgt aggtatcggt cgtgccgccc aagcggatga
     3241 tggcttgata gcgtttatcg gatgcagaaa catattcact taaacgaacg gcgggaccca
     3301 ctaatataac gagtacgcca gaagctctgg ggtctaatgt accagtatgc cctgcgcggc
     3361 gaagtcctgt accattgcga atggcttgta ctacatcgtg agaggtcatt cccacgggtt
     3421 tatcgataac gagcgcgcct gatatggcgt tctttacatc ttgactattc atcttacaat
     3481 cctcctactt cattgtcctt tttattatga catattcgtt atttttttca atgcattaat
     3541 agtgctgtag tactaaacta tacttacaaa ttcaataatt tttttgtaac gtttaaggcg
     3601 tcttttttaa tgtcagctaa acttccctca atatctgctc ctgcagcggc tgcatgaccg
     3661 ccaccgttga agtgtttggc gatcggcgaa acgtcaatgc ccgcctgaag agcacgccag
     3721 gagatcttta ctttattatc ggtctgttgt acaaagacca ttcccacttt atgtccttcg
     3781 atggcagaga tcatattgat caaatctgcg tcatcgttgc cgccataact tgctgacttg
     3841 cggtcttcca atgtcagcgt tccccaaaca atgccatctt tgctttcaag gcttgaaagc
     3901 ccggcgcccc aatacttggc agccgggaag gattttctca ctagcgattg catgtaaatt
     3961 tctggaaggt ccacacccga ttccattaaa gtcgcagctt gacgcaacgc ttcaggcgtg
     4021 acgttcgatg tgcggaagcc aagggtatcg gtcagaatac cggtcaataa agcagcggca
     4081 accggcttgg tgatcttgag cccccaagtg gggaggtggt tggtgagaat agctgctgtg
     4141 gcaacttctt ctgcttcaat tagatttagt ttcccaaatt tttcgttggt tttgtggtgg
     4201 tcaatattga tatcaggctg accaaagctt tcaaagattt tccccgttcg tttgaaatcg
     4261 gcacaatcta cagtaataaa agtgtcgtgt ccgccggtgg gttctttttt aatgagttca
     4321 ctcccttcaa gaaatttgaa agaagcgggc gctccatcta ccaggatcat ttcgacggtt
     4381 ttgcctgctt caataagagc cagtccaagt ccgagcaggg agccgatagc atctccgtct
     4441 gggcgtacat gcgatgcaat caccaccttc ttagcatctg cgagtcgatt ttttatatcc
     4501 cctgagatct gatctgtcac tataatttct ccacaattcc ttattttcaa taaggatgat
     4561 tattttttgt tccttaaccc tgccaacagt ttttcgatat ggtcagcatt ttcaggtgtg
     4621 ggatcccaat gaaatcgtaa ttttgggaat gaacgtagtt ctacacgggc agaaagggtg
     4681 cggcgcagaa acccagaagc ggattctagc cctgccagca cttcttcaga gcgggaagcc
     4741 ccttccacgg cagagacgta cacttccgca aaggcaagtt cacggtccac tttgatgccg
     4801 gtcacataaa tttgcttgag acgcggatca ctgatctcat gaatcagcat ttccgaaagt
     4861 tcctgctgaa tacgatcttc aattcgtttg attctaattc cagatggcat agtctttcca
     4921 aatgttacta cttacatatc actttgatac gtgatatgta agtagtaaaa gtacttattc
     4981 caagatgtag caaacgaggg tgtcgccaga ttggatgtcg gagaagttct tgaaaccaac
     5041 accacattca aacccttggc ggatctcttt cacatcgtct ttttcgtggc gaagagagga
     5101 gaggtcacct tcgtagatga tatctgagtc gcggtaaagg cgaaccttgg caccacgctt
     5161 caattcgccg tcggttacac ggcagcctgc cacacgtcca aacttcgagg cagtaaagac
     5221 ctgcaagacg gttgccttgc caagaacctt ttccttgaga tcgggctcca acataccatt
     5281 gagggctttt tcaatatcct cggtcatgcg gtagatgatc tcataaaggc gaatgtccac
     5341 gccttctttt tcagccatgc ggcgggcatc cacgtctgct tggacgttga aaccaatcat
     5401 gatggctttt gaggcggaag cgagcattac gtcattgttg ccgatattac ctgtttcggc
     5461 gtgtaacact ttcaacccaa tttcaccctg accgagctta tttaactcag taatgatggg
     5521 gtcaagcgaa ccttgcacat cggctttgac gatgaggttg agttctttcg cttcacccgc
     5581 ttggactttg ctaaacaggt cttcgagtga gagtttcttg cgagattgtg cctgagcctt
     5641 taagacttcc atacgttcag ccacaatggc acgcgcttct ttttcagacg ggacgacttc
     5701 gaacaaatct ccagcggagg gaacgtcact gagacccatt acagcaaccg gcgtggaagg
     5761 acccgccttc ttgaccggct tgcccttgta atcggtgatg gcgcgcaatt tgccatgcgc
     5821 ggttccagct acaaccacgt cgcctgattc gagcgtaccg ttctgaacga gcaaggtcgc
     5881 cagcacaccc ttggatttat ccaattccgc ttcgatgacg gtaccgatta ctttgccgct
     5941 ggggttggct ttgatctcgt tgctatctgc cacgagcaaa acgccttcga gcaaatcgtc
     6001 gatgcctttt ttctgcttgg cagagacggg cacgaccatg gtattgccac cccaatcatc
     6061 agggacgagt tcgagttcgg caagttgttg ttttacacgg tctgggttgg cattgggttt
     6121 atcgaccttg ttcaatgcca ccacgattgg cacacgcgcc gctttggcgt gcgcaatggc
     6181 ttctttggtt tggggcatca cgccatcgtc ggcagccact accaagatga cgatatcagc
     6241 accttgagca ccacgagcgc gcatctgcgt gaacgcggcg tgaccgggcg tatcgaggaa
     6301 ggtgatgaga cgccccttca tttccacttg atacgcgccg atgtgttggg taataccgcc
     6361 agcttcacca gcagccacat ctgtggaacg aatggcatct aacaagctgg ttttgccgtg
     6421 atcgacgtga cccagaatgg tcacaacggg ggggcgctgt ttcagttgag ccttatcttc
     6481 cccggcgatc atctgacgcc acatgggcac ttcgcccgct tcttcttttg attcttcttc
     6541 aacgacttcg ggtacggctt caaagccata ctcagcaacc acaatcgcgg cggtatcgta
     6601 atccaccgtc tgattaatgg ttgccatgac accattggtc atcaattttt tgatcaggtc
     6661 aatcgggctt ttttcgatct tttgagccag gtctcgtact acaatgctgg caggtaattc
     6721 gatcttattt ccgttttcac tcataagcca cctccttgaa tctcaaaaca tatctttgtt
     6781 caatgtatca ggcagcctgt gagcgagtgg cttattcgtt cacatggctt aaccgtcagt
     6841 ctctgcttct ggcagagtgt tcataaattc ttccaaacgg gcgcggtctt ctgcgctaat
     6901 ttccgttttc aaagcgtggg ctaaaccgcc ttttaatccg cgcgcccaac aagagcgctt
     6961 gtcgtgcaag taagcgcccc ttcctgccaa cttactggtg gggtccacac gcacgccttc
     7021 tgctgtgcgg acaattctca gtaattgtcg tttcgacagg atttcccggc acccgacaca
     7081 ggtgcgctgc ggaatatgtt ttacgcgttg tacaggtttt ttgaccacgt ccgtttactc
     7141 cccgcctcca taatggaggc gggaatcatt aactaccatg cctcgtcgtc gccgccatca
     7201 ctgcgtttgt gcttcttgcg tgagacaact tcgccaaggt tttcgtcgaa ctcgagggca
     7261 acacccttct tcttaacttt cttgcccttc ttcttatcgt cggattcttc atccgcacct
     7321 tcgccagctt ggaacatttc tggcttcatt tggaacagct cgtcgaggtt gacgccgtcc
     7381 ttcgagactt cgccatcttc ttcctcttct tcgatgacgc gtttcggttc tttcttcttt
     7441 tcggcgggct tctccacaac aggcgtttcc accactggag ctgtttctgt cacaacgggt
     7501 tctgcaacaa cagccggggt ttcctccacc ttgggttcag caaccggttc ggggaaggtg
     7561 agagccgcga gcgtttcttc gatgttcgta atggctttac caccaatacc agccagcccc
     7621 aacactttgt tctggtcggt cttcatatcg aagatcaact gaccaacggt ttcatagcca
     7681 gcttcagtaa gaatgttgaa gacgtgttcc ttgaggtttg ttgcctgatc gagcggcatg
     7741 ttgaacgcag cggcagggat ttcggcgcga gcggcggcaa tgcgttcgtc ttcttcgcga
     7801 gaagcctctt ccttggcaag gatcatgcgg cgttccacac ggtccacgaa ttgttcgagt
     7861 gtgctgaatt cttccggcgt aatgaggcga ttttcagcct tcttagcgag aatttcttcc
     7921 acctgcggca tcacttccac cgcagccttg agcatattct gcaattcggc atcgccctga
     7981 agtttggcaa gcgagtcgcc tgcagcttca gacaaagact tgatgtcgat gcgccagctg
     8041 gtcagtttgg cagcgaggcg ggcattttgt ccatcacgac cgatggcaag actcaactgg
     8101 tcttcgccaa ctacaacggt agctgtgcgt tcttcttcgg tgagatatac gccagtcact
     8161 ttggcggggc taatggcttt ggagatgtag gcaaccgggt cggcgtccca caaaatgatg
     8221 tcgatcttct catcgtgcaa ttctttgacg atggcttgaa tacgaacacc cttttgaccc
     8281 acacatgcgc cgacggggtc gataccgggt tgcgtggcag aaaccgccac tttggcgcgg
     8341 gcacctggtt cgcgggcaat cgcgcggatc tcaacaatgc catgatagat ctcaggcact
     8401 tcgttttcca tcaagcggcg caagaagtta cgatgcgcgc gtgaaaggat gatctgcgga
     8461 ccacgggttc cgtctttgac ttccatcacc accgcgcgga cgcggtcgtg tagcttcaat
     8521 ttttcaccag gaatgcgctg attattcggc atcacgcctt cggctttcat atccaagccg
     8581 atagttatgc cctgtgcatt gatcgcctgt accaaaccag agacgatttc gccttcctgg
     8641 cgctggtaat actccatctg attggagcgt tctgcttcac gaatacgctg ctggatgacc
     8701 tgacgagccg tctgtgccgc cacacgaccg aaatcagccg gggtgctttc caccacgacc
     8761 atatcgcccg gctggcactc agggttcacc ttgcgggcta cctcgagaag aacctcagtg
     8821 cgatcatcga cgatgctatc ttctacaact tccttctcag catagacggt tacaatcccc
     8881 gtttcggggt ccagcttgga ctccacatat tgagcggtgg atgcccccac agcccgccgg
     8941 tatgcggaaa ccatcgccga ctcgatcgcg gtgatgacca cgtccttggc gagttgcttt
     9001 tcttccagca cttcgttaaa tgctagcgca aaatcattct ttgccatcgt actgctccaa
     9061 aactccttta gatatcatac ccatagggta taaagcgaag aagtgggggc tgcccacttc
     9121 tcggtgacgt tcactattcg ggttgtccct tacgagcgag attctatagc agaaaggttt
     9181 cagcgtcaag gctgagtctg ttagaggagc atttgaactt ggcagtgtat aatcgccgcc
     9241 atgaccgaac gagatatgaa caaagccgcc ctgcgcggag aaccttcgta tgtatggcgc
     9301 ga
//
LOCUS       DAOQFT010000016         9203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3538_length_9203_cov_7.584390, whole genome shotgun sequence.
ACCESSION   DAOQFT010000016 DAOQFT010000000
VERSION     DAOQFT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3538_length_9203_cov_7.584390"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..454)
                     /locus_tag="PLR93_00750"
     CDS             complement(101..454)
                     /locus_tag="PLR93_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH25512.1"
                     /translation="MSVAPKSQILHIAEMVSYQEGSVVSRQITKEDAGNVTLFAFDKD
                     QGLSEHKAPFDALVHILEGEADVTISGTPYRLSAGDAIIMPANETHALKAIQKFKMLL
                     TMIRATSVAESGVKL"
     gene            complement(477..1691)
                     /locus_tag="PLR93_00755"
     CDS             complement(477..1691)
                     /locus_tag="PLR93_00755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH25513.1"
                     /translation="MKPTLDIPLLFATRIIRMFCYGFLSVILVLYLSETGLTEQQAGL
                     LFTFTLAGDAAISLYLTTHADGFGRKRTLIIGALLMLGAGVVFLMTNNIVVLMLAAII
                     GVISPSGNEIGPFLSVEQASLSQLLPDKMRTKVFAWYNLAGSFSTAIGALTGGWLAQL
                     LQASGWSALDSYRYILTGYAVGGLLIAILFLQVSPAIEVHSEPNETKKVLGLHKSRNV
                     VLKLSTFFALDAFAGGLIVQSMIAYWFHIKFGVDSGILGSIFFGANILAGISSLLAVK
                     IADKFGLINTMVFTHIPSNIFLILVPLMPSLPLAIGILLLRFSISQMDVPTRQSYTMA
                     VVAPDERSAASGVTAIARSVGASISPALTGIFFSIPGLLSVPFFLCGGLKIIYDLLLW
                     REFSAVKPPEEK"
     gene            complement(1693..2520)
                     /locus_tag="PLR93_00760"
     CDS             complement(1693..2520)
                     /locus_tag="PLR93_00760"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNH25514.1"
                     /translation="MKNKKSLLLVLLLLVSLACSLTGTPTPETSAPLESIAPTFTPIP
                     SFTPEAQTASAALSSGLTVQSSPVEEAGTEPPYTIKAQVPYLQGSDDARVQSFNAYLK
                     QIVQNEIDGFKTNTLAYASMPPMVNGSSLEIQYSVLGQRGDIWSIQFLVYFYADGAAH
                     PGHYSISVNYDLANSREVTLDELFLPGSDYLTTLADISKAELATRDIGFESFSTGADP
                     LPENYTRWNLSNEGFLVITFDEYQVAPYAAGPQTVTIPVSQLQTIVNPNGVLPLFGK"
     gene            2643..3368
                     /gene="rlmB"
                     /locus_tag="PLR93_00765"
     CDS             2643..3368
                     /gene="rlmB"
                     /locus_tag="PLR93_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNH25515.1"
                     /translation="MKEFIYSRNAVHEALLAKRRDIFSIEIAEGAQEKGTLAEIIKIA
                     QQRKIKLFRSPRPKLDKVNQHHQGVVAEVSGYPYADVVDILDKPKGEPPFILILDSLQ
                     DPQNFGTLIRTAEAVGVHGIIIPLARSVEVTPSAVNASSGASEHMLIAKSNLSQAMDA
                     LRDADIWLVGLDQAGAEVEANSRHLKGALGLVVGSEGEGIHELTRKKCDIVLKLPMKG
                     KIESLNAAVAGSVALYLAYLNRK"
     gene            3418..4743
                     /locus_tag="PLR93_00770"
     CDS             3418..4743
                     /locus_tag="PLR93_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNH25516.1"
                     /translation="MPQGTYHFPKGFLWGTATASHQVEGSNTNNNWWKWEQEGHTNGS
                     AAIAADWWGGRWREDFDRAAETGQNAHRLSIEWSRVQPTPDRWDEDAIERYRTMLRGL
                     RDRNMTALVTLHHFTDPLWVTERGAWETEEIVPLFEKYVRKMVDSLKEYTNLWVTINE
                     PNVYALSGYVTGDFPPGKKDLKQAMPVIANMVRGHAAAYRAIHELQPEARVGYALHFR
                     PMVPRLSWSPLDRLMRNIRYEGVNMGFPTAISTGVMKTPIGNFNIPEAKGTQDYLGLN
                     YYSVDTVWFDLAKADQMFSNSGYPKDADMSDTNFLANIPHGIYDSIKWIVRTYPNLPI
                     IVTENGVEDFDDHMRPRYLVQHLHEVWRTVNFNWPVKGYFHWSLVDNFEWERGWTQRF
                     GLWGLDLDTQKRIRRPSVDLYAEICKENGISSEMVQKYCPEVFEKLFPS"
     gene            complement(4846..5748)
                     /locus_tag="PLR93_00775"
     CDS             complement(4846..5748)
                     /locus_tag="PLR93_00775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25517.1"
                     /translation="MITYRRATPADNFTTFTIFRQSLEDFSKRTGVMAITGGNDPQKM
                     DELWESRRSFWEHLTNTSDNYWIAEKDGEAIGYARSILRGNHRELTEFFVVPGNQSVG
                     VGKELLQRAFPDDVPHRTIIATPDIRAQTRYLKAGVYPFMTEFYFERTPEPQTFETDL
                     VIELAEASPASSDDLGRIDLSILGFRRDIDHQFLTSDRKLYFYKRNGQVVGYGYINRD
                     LYGPFALLEKEDFPAVLAHAENEAHKMGASVVGFEVPTINSSAISHLLNRGYRLEGFL
                     GSIMSEKPFGRFEHYVLTSPPYFL"
     gene            complement(5784..6533)
                     /locus_tag="PLR93_00780"
     CDS             complement(5784..6533)
                     /locus_tag="PLR93_00780"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNH25518.1"
                     /translation="MSHAKFQKYHREVAQALTSIGNPAFGKAVQKDRGSHLKHLGIKF
                     PELRRRVKQGFSFYALPEEQILEIWDALWQTSPYGDVLFAALEYYIPIVKKQVPANLW
                     GVVKGWSLRIDNWCHSDALCSVYSRILEGNQKEVYPQLLEWNQAQSEWLRRISLISLI
                     HYSGKNAVFISPKKVLPLVSNCLDDERYYVQTAVGWVLREMGQMYQGEIQKYLETHVE
                     RLSSPAFSRSIERLSTKERTHLQGIRKRRMA"
     gene            complement(6565..7734)
                     /locus_tag="PLR93_00785"
     CDS             complement(6565..7734)
                     /locus_tag="PLR93_00785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH25519.1"
                     /translation="MTQTYDAIVIGAGVMGASLAFHFAERGLKVAILERKVTASGATG
                     HSSGLVRMHYDLAAESHLTFASYKNYFSIWKERVGGECGFQQTGFLQIAKREHEDKLR
                     GNVANQQKIGINTSVISVDEVRKLFPDLVTEYFDYAAYEPDSGYADATLTTNSFLDSA
                     KRNGATLIQNCEVTAIHVSGVKVTGVSTTQGDFSAPIVVNAAGPWAKHVAALAGIEVP
                     LVTWTHDVAFLHRPPTLGKIPAVIDDVINCYFRPEGSALILAAGEDESLRGEAPDAED
                     QTPTPTFLDKLIDQMVQRIPKIEESGLHSVHVGRDGITPDQRAIYSGTDLGGFYLACG
                     LSGTGFKTSPAAGASMVELILDGTPKTVDITPFRFSRFADGTLLEGEYGYGNIWK"
     gene            complement(7812..8237)
                     /locus_tag="PLR93_00790"
     CDS             complement(7812..8237)
                     /locus_tag="PLR93_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNH25520.1"
                     /translation="MSAKHNPFIKLFWSIHLKLYLWSSGRIGNTIRGLPVLLLTTKGK
                     KTGQLRTKALMYLPYGDAFVVIASNLGKDNHPAWWLNLQADSTATVQVKDARFTVRAR
                     EAEGDEREKLFQALAQKTSDYEQYRSWTSRRIPVVILER"
     gene            complement(8239..>9203)
                     /locus_tag="PLR93_00795"
     CDS             complement(8239..>9203)
                     /locus_tag="PLR93_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNH25521.1"
                     /translation="TPPTETLTPPIPTASLTPSPIPPAPETPQPTVTIPAPQPISRVF
                     IVTFDGLRPDAIDAAGMTNVQGMMKNGAYTLTAQTIMPSTTLPAHSALVTGTCPAKNT
                     VRWNEYVPQNGYALGTDIFDLTRSVGLRTVLVVGKEKLRQITESTDFFGWVDNTDEAK
                     DKTTLDNMALEQIDLGFGLMMVHFPDGDVVGHEYGWLSKQQLSAYKKNDQSFGRFISA
                     MKKNGYYDGTLFIITSDHGGALTDPPHGTNIPEAMTIPWIISGPQIVPGELKTPVYIF
                     DTAPTVAFALGLPLQAEWDGTPVYEAFGLPVDPLHQGGCPGLTP"
BASE COUNT         2376 a         2158 c         2428 g         2241 t
ORIGIN      
        1 ctcctccttt ggcgaaagct gacgtcaggt gaacagcctg acgtcagcat tttaattctt
       61 gttagcgcgc gggcacgtcc aacaactata gattttatgt ctacagtttc accccactct
      121 cggcaacaga tgttgctctg atcatagtca gcaacatctt gaatttctga atagctttca
      181 aagcatgtgt ttcattggcg ggcataatga tggcatcccc tgcggagaga cgatagggtg
      241 tgccggagat ggtgacatcc gcttcgcctt cgaggatgtg gacaagagca tcgaagggag
      301 ctttatgctc gctcaagcct tggtctttat cgaacgcaaa caaggtgaca ttgcccgcat
      361 cttcttttgt aatctggcgg ctgaccaccg agccttcttg ataactaacc atttcagcga
      421 tatgcagaat ttgtgatttg ggtgcaacag acataaggtt ctccaagggt gacaaactat
      481 ttttcttctg gcggtttgac ggcgctaaac tcacgccaga gtaacaggtc gtaaataatc
      541 tttaatccac cacagaggaa gaatggaacg cttaacagtc ccggaatact gaaaaagatt
      601 ccggtcaatg cgggggagat ggaagcgcct acagaacgcg cgatagcggt cacgcctgag
      661 gcagcggatc tttcgtctgg agccacaaca gccatcgtgt aggattgacg cgtgggcaca
      721 tccatttgag agatgctaaa gcgcaggagg aggattccga tggcgagagg cagagaaggc
      781 atcagcggca cgagaatcaa gaaaatatta gaaggaatgt gggtaaagac catggtattg
      841 atcaacccga atttatccgc gatctttact gcaagcaaag atgaaatacc cgccagaata
      901 ttcgcgccga agaagatgct gccaagaata cctgaatcca cgccgaactt gatgtggaac
      961 caatacgcaa tcatgctttg aacaataagc ccgcctgcaa atgcatcgag ggcaaagaag
     1021 gtgctgagct tgagaaccac gtttcgtgat ttatgcaagc ccaaaacttt tttggtttca
     1081 ttgggttcac tatgcacttc aatagcaggg gaaacttgta aaaacaaaat cgcgatcaac
     1141 aagccaccca cggcataccc tgtaagaata taccgatacg aatccaaagc agaccaaccc
     1201 gaagcttgca aaagttgagc gagccacccg cctgtaagcg caccaatggc tgtggagaat
     1261 gaaccagcaa ggttatacca agcaaagact ttggtgcgca tcttatctgg caaaagttga
     1321 cttaggcttg cctgttccac cgagaggaag ggaccaatct catttccgct agggctaatg
     1381 acaccgatga tggcggcaag catcaagacc acaatgttat tcgtcatcaa gaaaacaaca
     1441 cctgctccca acatcaacaa agcgccgatg atgagggtgc gtttccgccc gaagccatca
     1501 gcatgtgtgg tgagatacag cgagatggcg gcatccccag caagagtaaa ggtaaagagc
     1561 aaaccagctt gttgttctgt gagcccggtt tcagaaagat atagcacgag aataaccgac
     1621 aagaacccat aacagaacat acggatgata cgagtggcaa acaacaatgg aatatcaagt
     1681 gtgggtttca tgttattttc caaatagtgg taatactcca ttcgggttga caatggtttg
     1741 caattgagaa acaggaatgg tcaccgtttg cggacctgct gcatagggcg cgacttgata
     1801 ttcatcaaag gtgatcacaa gaaacccttc gttcgaaaga ttccagcgtg tgtaattttc
     1861 aggcaatggg tctgcaccgg tagaaaaact ttcgaagcca atatcgcgtg tggcaagttc
     1921 ggctttcgaa atatcagcaa gggtcgttag ataatcagaa cccggcagaa aaagttcatc
     1981 caacgttacc tcacggctgt ttgccagatc ataattgacc gagatgctgt aatgcccagg
     2041 gtgcgctgcg ccatcggcat agaaataaac aaggaactga atactccaaa tatcgccgcg
     2101 ttgcccgaga acgctgtatt gaatttcaag cgagcttccg ttcaccatgg gcggcatgct
     2161 ggcatacgca agcgtgttgg tcttgaagcc atctatttca ttttgaacaa tctgcttgag
     2221 atacgcatta aagctttgga cacgcgcatc atcagaacct tgcaagtaag ggacttgggc
     2281 tttgatggtg tagggcggtt cagtccccgc ctcttccacc ggcgacgatt gaaccgtcag
     2341 cccagagctt agcgccgctg aagctgtttg tgcttccggc gtaaacgagg ggatgggcgt
     2401 gaaggtcggc gcaatacttt ctaacggagc agaggtttct ggggttggtg ttccggtcag
     2461 cgagcaggcg agtgaaacaa gcaaaagcaa taccagtaaa agagactttt tgtttttcat
     2521 tagaactcct tgagataatt gaattctatc gcttaactta attagtgttt ggttcggaat
     2581 gtttagtgtc ctcctgcggt ttgatataaa acaagcgtac atcaagtaaa atacactttg
     2641 atatgaaaga attcatctat tcacgcaatg ccgtccatga agccttgctt gccaaacgca
     2701 gggacatttt ttcaatagaa attgccgaag gggcacaaga aaaaggcaca ctggcagaga
     2761 tcatcaaaat tgcacaacag agaaagatca agctattccg gtcgccgcgc cccaagttag
     2821 acaaggtcaa ccagcaccat caaggtgtgg tggcagaggt gagcggctat ccctatgcag
     2881 atgtggtcga tattctggat aagcccaaag gcgaaccgcc tttcatcctc attcttgact
     2941 cgttgcaaga cccgcagaac tttggtacgc tcattcgcac tgcagaagcc gtgggtgtgc
     3001 atggcattat cattccgctg gcgcgctcag tggaggtgac accctctgcg gtgaatgctt
     3061 cttcaggcgc gagtgagcat atgctcatcg ccaagtcgaa tctttcgcag gcaatggatg
     3121 cgcttcgaga tgcagacatt tggctggtag ggttggatca agccggggcg gaggtggagg
     3181 cgaattcccg tcatctcaaa ggagcattgg gattggtcgt cggctcggag ggtgaaggca
     3241 tccacgaact gacgcgcaag aagtgtgaca ttgtgttgaa gttgccgatg aagggcaaaa
     3301 ttgaatcttt gaatgctgct gtggcagggt cggtggcatt gtatttggcg tatttgaata
     3361 gaaaataatg tgtaggggcg acttgcaagt cgcccctacg atactatgga gatcatcatg
     3421 cctcaaggca cttatcattt tccaaaaggg tttttatggg ggacggcgac tgcttcgcat
     3481 caggtagaag gcagtaacac aaataacaat tggtggaagt gggagcagga aggtcacacc
     3541 aatgggtcgg cggcaatcgc tgctgattgg tggggcggac gttggcgcga agatttcgac
     3601 cgtgcagccg agacgggtca gaatgcacat cgtctgtcca ttgagtggag ccgtgtgcag
     3661 cccacacctg atagatggga tgaagacgcc atcgaaagat atcgcaccat gttgcgcggg
     3721 ctgcgtgatc gcaatatgac cgccttggtg acgttacatc acttcaccga cccgctgtgg
     3781 gtgacggaac gtggcgcatg ggaaaccgaa gagatcgtgc cgttgttcga aaaatatgtg
     3841 cgcaagatgg tggattcttt gaaggaatac accaacctat gggtgacgat caatgaacca
     3901 aatgtctatg cgttgagcgg atatgtaacg ggagatttcc cgcctgggaa aaaagactta
     3961 aagcaagcca tgccagtcat cgccaatatg gtgcgcggac atgccgccgc ctaccgtgcc
     4021 attcatgagt tgcagccaga agcacgggtg gggtatgcct tgcatttccg cccgatggtg
     4081 ccgcgtttga gttggtcacc tttagaccgg ctaatgcgca atatccgcta tgaaggcgtg
     4141 aatatgggct tccccactgc aatctctacc ggtgtgatga agactccaat cggcaatttc
     4201 aacatccctg aagccaaagg gacgcaagat tatctgggct tgaactatta ctccgtggat
     4261 acggtttggt tcgatcttgc aaaggcagac cagatgtttt caaacagcgg ctaccccaaa
     4321 gatgccgata tgagcgacac gaattttctc gcgaatattc cgcatggcat ttacgactcc
     4381 atcaaatgga tcgtgcggac ctatccaaac ctgcccatca tcgtcactga aaacggcgta
     4441 gaagatttcg acgatcacat gcgtccgcgc tatttggtgc agcatttgca tgaagtttgg
     4501 cgcacggtca atttcaactg gcccgtgaag ggctacttcc actggtcgtt ggtagataac
     4561 ttcgaatggg agcgcggttg gacacaacgc ttcggtctgt ggggcttgga cttggatacc
     4621 cagaaacgca tccgccgccc gtctgtagat ctgtatgctg agatctgtaa agagaatggg
     4681 atttccagcg agatggtgca gaagtattgc cccgaagtgt ttgagaagtt gtttccttcg
     4741 taagttttat taatgtcacc ctgagcggac cgaagggtct ctacctatgg gtcagagact
     4801 cttcggtcgc gaagagcacg cttcctcaga gtgacatagc ggtactcaca aaaaataggg
     4861 cggactggtc agaacgtaat gttcaaatcg tccaaatggt ttttcagaca tgatcgagcc
     4921 aagaaagcct tcgaggcggt atccgcgatt gagaagatga ctgatggcag atgaattgat
     4981 ggtgggtact tcaaaaccta caacagacgc acccatcttg tgcgcttcat tttctgcatg
     5041 agccagcacg gcaggaaaat cttccttttc aagcaacgca aaaggaccat ataaatctcg
     5101 gttgatatac ccgtaaccta cgacttgtcc gttacgtttg tagaaataca acttacggtc
     5161 actggttagg aattggtggt caatgtcgcg gcggaaacca aggatcgaga ggtcgattct
     5221 gcctaagtcg tcggaagagg cgggcgaagc ttctgctaat tcaataacaa ggtctgtttc
     5281 gaaagtctgc ggctcgggag tacgctcaaa ataaaattcg gtcatgaagg gatatacgcc
     5341 cgctttgaga taacgcgttt gagcgcggat atcgggagtg gcaatgatgg tgcgatgcgg
     5401 cacatcatcg gggaaggcgc gctggagcaa ttccttgccc acccccacag actgattgcc
     5461 cggcacaaca aagaactcgg tcagctcgcg atgatttccg cgcaagatgc tgcgggcgta
     5521 gccaatggct tcgccatctt tttcggcgat ccaataatta tcgctggtgt tggtgagatg
     5581 ctcccaaaaa ctgcgacggc tttcccaaag ctcatccatt ttttgcgggt cattgccgcc
     5641 agtgatcgcc atcacgccag tgcgtttact gaaatcttcc aacgactgac gaaagatagt
     5701 gaaggtagtg aaattatctg cgggggtggc tctgcggtaa gtgatcatag ttggacattt
     5761 tagcagagtt tgattttatg cgatcatgcc atgcggcgtt ttcgaatgcc ttgtaagtgt
     5821 gttcgttctt tcgtgctcaa ccgttcaatg gaccttgaaa aagcaggcga acttaatcgt
     5881 tctacatgag tttcaagata tttctggatt tcgccttgat acatttgccc catttctcgc
     5941 aaaacccagc caaccgccgt ttgaacatag tagcgttcat catctaaaca attcgacacc
     6001 aacggcagga cttttttcgg cgatatgaag actgcatttt tgccgctgta atgaataagg
     6061 ctgatgagag aaatgcgcct caaccattca ctttgggctt gattccactc taggagttgg
     6121 ggatacactt ctttttgatt gccctctaaa atgcgcgaat acaccgaaca aagcgcatcg
     6181 ctatgacacc aattgtcaat gcgtaaagac caacctttga cgacgcccca cagattggct
     6241 ggaacttgtt ttttcactat gggaatatag tattcaagcg cggcaaacag cacatcgcca
     6301 tagggcgaag tttgccatag cgcatcccaa atctctagta tttgttcttc tggcaaagca
     6361 taaaaagaaa aaccttgttt cactctccgc cgcaactcgg gaaatttgat accaagatgc
     6421 ttaagatgcg agccgcggtc tttctgcaca gctttgccaa acgctgggtt gccaatggat
     6481 gttagagcct gcgccacttc gcggtgatac ttctgaaatt ttgcgtggct catcaggcaa
     6541 tattctcaat gtgtggcaaa gaaattactt ccaaatattt ccatatccat attcgccttc
     6601 gagcaaagtc ccatcggcga aacggctaaa ccggaacgga gtgatatcca cagttttggg
     6661 tgtgccatcc aaaatcaatt ccaccatgct agcaccagca gcaggtgacg ttttgaaacc
     6721 agtgccactc aaaccacaag cgagataaaa cccgcccaag tctgttccgc tatagatggc
     6781 acgttgatcg ggcgtaatgc catcacgccc aacatggaca gaatgcaagc cgctctcttc
     6841 gatcttcgga atgcgctgta ccatttgatc aatgagctta tcaagaaacg ttggggtggg
     6901 cgtttggtct tcagcgtctg gcgcttcgcc acgcagtgat tcatcttcac cagcggcaag
     6961 gatgagcgcg ctgccttccg ggcggaagta gcagttgatg acgtcatcga tcacagcggg
     7021 gatttttccc aatgtgggcg ggcgatgaag aaaggcaaca tcgtgagtcc atgtcaccaa
     7081 tgggacttcg attcctgcca acgccgcaac atgtttcgcc cacggtcccg ccgcattgac
     7141 gacgattggg gctgagaagt cgccttgggt cgtgcttacg ccggtcacct ttacgccact
     7201 gacatggatg gcggtcactt cgcagttttg aatcagcgtg gcgccgtttc gttttgcaga
     7261 atcaagaaaa gagttggtgg tgagcgtcgc atcagcatag ccagaatccg gctcataagc
     7321 agcatagtca aagtattctg tcacaaggtc tggaaaaagt ttgcgaactt catccaccga
     7381 aataaccgag gtgttgatac caatcttttg ttggttcgcc acattgccac gcaatttgtc
     7441 ttcatgctcg cgtttggcaa tttgcaagaa accggtctgt tgaaaaccac attcgccacc
     7501 tacacgctct ttccaaatgc tgaaatagtt tttatagctg gcaaaggtca ggtgtgactc
     7561 agcggcgaga tcgtaatgca tgcggacaag cccgctggag tgcccggtgg caccagaggc
     7621 tgtcaccttg cgttcgagaa tggcgacttt cagtccgcgc tcggcgaagt ggaaagcaag
     7681 gctggcgccc atcacaccgg caccgatcac aatggcatcg taggtttgag tcatagaaga
     7741 gctccttttg ccgcgaattg tgcggttgag tttttttggt tgcgaggaat tttagcagag
     7801 tggggcgatt gttatcgttc caatatcacc accggaatac ggcgggacgt ccatgagcga
     7861 tattgttcgt aatcagaagt tttttgtgcc aatgcctgaa aaagtttttc acgctcgtcc
     7921 ccttcggctt cgcgagcacg aaccgtgaag cgtgcatctt tgacctgtac tgttgcggtt
     7981 gagtctgctt gcaggttaag ccaccaagcg gggtgattat ctttcccaag gtttgaggcg
     8041 atgacaacaa aggcatctcc atatggcaaa tacatcaagg cttttgtacg gagttgcccc
     8101 gttttcttgc ctttggtggt tagaagaagc acggggagtc cgcgaatggt attgccaatg
     8161 cgacctgaac tccacagata aagcttgagg tgaatggacc aaaatagctt gataaaaggg
     8221 ttatgtttgg ctgacatact atggggttaa gcctgggcaa ccaccctgat ggagcgggtc
     8281 tacgggtaag ccaaacgcct cgtaaacggg cgtaccgtcc cattccgctt gcaagggcaa
     8341 gcccagggca aatgccacgg ttggagctgt atcgaaaata tagaccggcg tttttaactc
     8401 accggggaca atttgcggtc cgctaatgat ccagggaatg gtcatggctt cgggaatatt
     8461 tgtgccatgt ggagggtcgg ttaaggcacc accatggtca gatgtaatga tgaacagcgt
     8521 gccgtcataa tatccgttct ttttcatggc actgataaaa cgcccaaacg attggtcgtt
     8581 tttcttgtaa gcgctcaatt gctgtttgga aagccagcca tactcatgcc cgaccacgtc
     8641 cccatcgggg aagtgcacca tcatcagccc aaaacctagg tcaatctgtt caagcgccat
     8701 gttatctaag gtggtcttat ctttagcttc atcagtgtta tccacccagc cgaagaagtc
     8761 tgtgctttct gtgatctggc gcagtttttc cttgccaacc acgagcacag tccgcaaacc
     8821 gacagaacgt gtgaggtcga agatatcggt gcccaatgca tatccatttt gtgggacata
     8881 ttcattccag cgcactgtgt ttttggcagg gcaggtgcca gttaccaaag cggagtgagc
     8941 gggtaaggtc gtgctgggca tgatggtctg tgcagtgagc gtgtaagctc cattcttcat
     9001 catcccttgg acgttggtca tcccggctgc atcaatggcg tcggggcgaa gtccatcaaa
     9061 ggtgacgatg aaaacgcgtg agataggttg tggcgcaggg atagtaactg tcggctgggg
     9121 cgtttcaggc gcaggtggaa ttgggctggg tgtcagcgag gcggtaggga ttgggggtgt
     9181 aagagtctcg gtcggagggg tgg
//
LOCUS       DAOQFT010000017         9181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3549_length_9181_cov_7.195814, whole genome shotgun sequence.
ACCESSION   DAOQFT010000017 DAOQFT010000000
VERSION     DAOQFT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3549_length_9181_cov_7.195814"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLR93_00800"
     CDS             complement(<1..1153)
                     /locus_tag="PLR93_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH25522.1"
                     /translation="MSRKIIPFVIALILICLVMGVAGVFAFSGFAAAEKFGSQTAWSQ
                     SYSQAESMKVIDVTGDGQDDLFIQNASEVNILDGSGTPILGLDYPSPKTTLGDVNGDG
                     VEDIVVYYMGPEGASVDVISKGSVSNLAKSLNIGSPARVAFIRFSSGPQIVLGDSSGN
                     VLSIDAGSGAPLWNSNVGSAEIRGMDDARISGEVHVAIASYDGTVKLFNSAGETVWSA
                     EQEDLRRMRAFDLNADGNSEVITGGEYGLFRIYSAAETKVLFEKSLGQAVSEVREVEL
                     NGDPSSREIMVGGKDGGVWAFSFNGTTATQMWTGSLSDKVTEIAGLDINEDGKQEAVI
                     GDDSGKVAVFTDNGTRNNLPDHSTGITRIDIGKLGSERYVVIADYNEVQT"
     gene            complement(1154..2104)
                     /locus_tag="PLR93_00805"
     CDS             complement(1154..2104)
                     /locus_tag="PLR93_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25523.1"
                     /translation="MDGLICGLIIAGIVVISFFFYLLNTARKKGNTDKNQFGKTEYSP
                     VYVSIADKPDSIIRGMNKFVAEARKTETAGDKWRWIPMLIFFAGIGLALIDVFILLLG
                     YSISFVFIGGGVLLWIAAFVMARALRRSDLLDFSPRYNGTKEILHTLRDDLKPGSTFL
                     GHLDLTGSMLQNKVARETQDAQSRTTQLFNDPWLSLKAKLYDGNVLRVTAIQKTKKRK
                     SYWKRSRISGKSKLKPEKFKGSQQELKVRIVVNPEAYVIRPNNQFSLNKDVGKYKVAQ
                     LSTEGGMINILAVSPFEDVSHTDILTFLKSAYSLLERKAA"
     gene            2172..3335
                     /locus_tag="PLR93_00810"
     CDS             2172..3335
                     /locus_tag="PLR93_00810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH25524.1"
                     /translation="MKNVLYTAFDIVPSPKGASTHILHNLRGLVNGNFNVHLITPNDG
                     VLPPEDTIEGARLTRISQDLSQNFLARAVHFGKSVLSHLALNPSYDVVHYRNIWDGFH
                     ITQNKKKFGYKTIFEVNGLSSIELKYHYPGMDASLLAKIKEQELATLHLSDAIICPSN
                     VTRDYIASLGLNRKLVTVIPNGVSPSDFPVTPLPIRDGREPVLLYIGTLADWQGLDIV
                     IKALPKILEQQPVKLRILGRGRSRQRKMLAKQIRKLGLDEYVLVQPAVPHHEIPAVIA
                     SADLCVAPLGLNDRNVTQGACPIKVLEYMAAGRPLLASNMPIVRELVREDVDGLLFSP
                     NDPDDLARQANQLLKDMELSKRLAESAAGYVREKFTWHESQKKLLKVYEKLIK"
     gene            3403..4407
                     /locus_tag="PLR93_00815"
     CDS             3403..4407
                     /locus_tag="PLR93_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25525.1"
                     /translation="MLDFIKEAGFPAIALLLGGVMVVIAIIRNIALEKVKIQLTSSQS
                     IQLAVVGVLFIIGGFVLQYFPPSSANTPENVDVYSTLYALQTQMVQPVNPQGQTNQTS
                     TPAGFALVSPTVTLFPNPVATLTPTLIPFTLTPDPSQPLAEVFCTNFYSIMVREGPND
                     NYPIKSVIENPNNLSNPDCMLFDFRMPDNSWIRIAANQDNPNYTQHELGWVTSDQFRP
                     IDFDKLRVYIPENVQDGLYCVTSRYGLKVRSCPNEGCREVGNLSLQDCLFMDGRSSDG
                     LWVRVSADKNDEKYASYKGNWVSTYFLAPFEFSAGYQPYFRYYFELLPTVEQIPTPNG
                     "
     gene            complement(4447..4851)
                     /locus_tag="PLR93_00820"
     CDS             complement(4447..4851)
                     /locus_tag="PLR93_00820"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNH25526.1"
                     /translation="MPISSIYSKTITIPSSTIDENGHVNNVVYVQWMQDIAIEHYASI
                     GGVEAQGPDATWVIRKHSIEYFLPAFEGEEIEVRTWVEDLRKVRSLRMYEFVRKSDGK
                     TLVKGESDWVFMDMKTGRPKAIPAEVENIFKP"
     gene            4951..5373
                     /locus_tag="PLR93_00825"
     CDS             4951..5373
                     /locus_tag="PLR93_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25527.1"
                     /translation="MGPIIGGTSLIITVISIACGCLITIVTLAGTGFLIYKVFGSTFK
                     SMSTNRNVLQTGVSAPAVILSVQDTGVTIMNNNPQARIRLRVMPMNGEAFEAEVTQII
                     GRFQVGMVVPNGSAMVKYDPNDKTKVAIESFGMPSAGQ"
     gene            complement(5422..6039)
                     /locus_tag="PLR93_00830"
     CDS             complement(5422..6039)
                     /locus_tag="PLR93_00830"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNH25528.1"
                     /translation="MKSRTILISLILVIGAFLFSCANNQADQTTALDTDAIRTQAVET
                     YASNLTQLAPTYTPFPTLTPVDITATALSLTPEPSPTSNPCFNLLYIDDVTVPDGTRM
                     KPGEKFTKTWLVQNIGGCAWRAGFTFQNVGGDSMRGTPITLTEAIQTGAKRELSVELV
                     VPSGINGVIESAWQMADDTGVFFGDTLFVSIVVGDVTTPAVTSTP"
     gene            complement(6041..6442)
                     /locus_tag="PLR93_00835"
     CDS             complement(6041..6442)
                     /locus_tag="PLR93_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25529.1"
                     /translation="MKIRDASGWTMFIFGVMAFLLGILGIIRPELQLAMLGFEVVERA
                     ARASSDYTLTFMTAASMASLNMGIYYILAALNDVKIFYRWTVPFRGLTFLVFTTVVIL
                     GIAPLKFIGVPIWELTGAIATGIALAREKQK"
     gene            6519..7169
                     /locus_tag="PLR93_00840"
     CDS             6519..7169
                     /locus_tag="PLR93_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNH25530.1"
                     /translation="MAKHLVLVAGNIGAGKTTLTERIGARLGWWTGYESVADNPYLSD
                     FYGDMRSWSFHLQVFFLGHRADQYLEAARDARSAILDRSIYEDAHIFARALHHLGNLN
                     ERDYLSYRKLFELVVNGLPRPDLLIHLKAPVPVLMDRIRRRARNMETGISAEYLTLLE
                     SFYDEWLGSFDLCPVLTIQTDDLDYVNYPQHLDQVAQRIQDKLAGREMMDLRGDNK"
     gene            7169..8416
                     /locus_tag="PLR93_00845"
     CDS             7169..8416
                     /locus_tag="PLR93_00845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH25531.1"
                     /translation="MNEKMDSSVFLKIPLFKDLPKAELYSLVSELPVEKYQAGDYIFH
                     EGEPGNSLYVVQKGKIDVVLAGGTNDEMLLRTCGPGEYVGEMSLILKQGKRTAAVRVR
                     EDSQLWMMTRDKFNECLQRWPHLAYAMVEIISERLDSSNEAAFNDLVKKNQMLQKAYD
                     DLKAAQAQLIEKERLERELQLAADIQLSILPDVLPDTEEFGFGARILPARQVGGDFYD
                     VFQLKDDRIGVLIGDVADKGVPSALFMARAHALIMAEADIGATPAEVMNLVNSHITRL
                     QKSTQFVTVLYGILDLKTRIFSYARAGHEPPLVLHTDGSVERIPHSPGMAIGLWEPVT
                     LDEKTVKLSSGDTLLLYTDGMTDCRDPQGDAFGLERIKDKLGELSKQSAQQICNTLLE
                     TLQNHQNGAKQDDDVTLVAVKAK"
     gene            complement(8447..>9181)
                     /locus_tag="PLR93_00850"
     CDS             complement(8447..>9181)
                     /locus_tag="PLR93_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNH25532.1"
                     /translation="STRIVWLETPTNPLLAVSDIRAVAEVVKNHPNKPLLVVDNTFAT
                     PYLQRPLELGADLVVHSMTKYLGGHSDVVGGAIIGKDKELGEKLAYLQNAIGGVQGPM
                     DSFLVLRGIKTLPIRMDRHAQNAEKIVAFLEKQKNVKRLIYPFHESHPQVQIAKRQMK
                     NGGGMISFIMKDGRDAAVKVAEATKLFTLAESLGGVESLIEVPAAMTHLSTQGSTLEV
                     DPGLVRLSVGIENVDDLIADLEQALR"
BASE COUNT         2324 a         2178 c         2240 g         2439 t
ORIGIN      
        1 tcgtttgcac ttcattgtaa tcagcgatca ccacataacg ctcactgcca agtttaccga
       61 tgtcgatgcg ggtgatacca gtggaatggt cgggcaggtt gttgcgtgtg ccattatctg
      121 tgaagaccgc caccttgcct gagtcgtcgc cgatgaccgc ttcttgcttg ccatcttcgt
      181 taatgtctaa gccagcgatc tcggtcactt tatcagagag ggagcctgtc cacatttggg
      241 tggcagtggt gccattgaat gagaacgccc aaacgccgcc gtctttgccg ccaaccatga
      301 tctcacggga agatgggtca ccgttcaact ccacttcgcg cacttcgctt acggcttgtc
      361 ctaaagactt ctcgaaaagg accttggttt ctgccgcgct gtagatacgg aacaagccat
      421 attcgccgcc ggtgatgact tcgctattac catcggcatt gagatcaaat gcccgcatcc
      481 ttcttaagtc ttcctgttct gccgaccaga cagtttctcc agccgagttg aagagcttga
      541 ccgtcccatc ataagaagcg atcgccacat ggacttctcc gctgatacga gcatcgtcca
      601 tgccgcggat ttctgccgag ccgacgtttg aattccaaag cggcgcaccg ctcccagcat
      661 caatcgaaag gacgtttccg ctgctgtcgc cgagaacgat ctgaggtccg ctagagaagc
      721 gaatgaatgc cacacgggca ggggagccga tgttaagcga tttcgccagg ttgcttacac
      781 tgcccttgga gattacatcc acagaagcgc cttcgggacc catatagtaa acaacaatgt
      841 cttccacccc atcaccgttg acatctccaa gagtggtctt gggggagggg taatccagcc
      901 cgagaatggg tgtaccagac ccatcgagga tattgacctc gcttgcgttt tgaataaaga
      961 ggtcatcctg accatcaccg gtcacatcaa tgaccttcat gctttctgct tgcgaatagg
     1021 attgagacca tgcggtttga ctgccaaatt tttcggcggc ggcaaatccg ctaaaagcga
     1081 acacgccagc aacccccatg acaagacaga tcaagattaa tgcaatgacg aacggaataa
     1141 ttttgcgact catttatgca gcctttcttt ccaataatga ataagcagat ttgaggaagg
     1201 tcaaaatatc agtatgactg acatcttcaa aaggagaaac agcaaggatg ttgatcatgc
     1261 cgccttcggt gctgagctga gcgaccttat acttgccaac atctttattt agcgaaaatt
     1321 gattgttcgg gcgaatgaca tacgcctcgg ggttgaccac aatgcgcact ttcaattctt
     1381 gttgtgagcc cttgaacttc tcaggcttca acttggattt gccgctgata cgactgcgct
     1441 tccaatacga tttgcgtttt ttggtctttt gaatggcagt aacacgaagc acattgccat
     1501 catacaactt ggctttgagc gaaagccaag ggtcgttaaa aagttgggtt gtgcggcttt
     1561 gagcatcttg tgtttcgcgg gctactttat tttgcaacat ggaacccgtc aggtcgagat
     1621 gaccaaggaa tgtagagcca ggtttgaggt cgtctcgaag cgtgtgcaga atttcttttg
     1681 tcccgttata gcgcggagag aaatccaaca agtcactgcg gcgtaaggca cgtgccatca
     1741 caaaggctgc gatccaaagt agaacaccgc cgccaatgaa gacaaaggag atgctatacc
     1801 caagtagtaa gataaaaaca tcaatgagtg ccagcccaat gcccgcaaag aagatcagca
     1861 tcggaatcca acgccactta tccccggctg tttcggtctt tcgcgcttca gccacaaact
     1921 tgttcattcc gcgaatgatc gagtctggct tgtctgcaat cgaaacatag acaggtgaat
     1981 actctgtttt gccgaattga tttttgtcgg tgtttccttt tttacgtgcg gtgttcagca
     2041 ggtaaaagaa aaaagagatg acaacgatcc ccgcgatgat aagcccacaa atcagaccat
     2101 ccatccatgc ctccgagtta tttttatgcc cacagtcgca atttgtgacc gcgctgggta
     2161 taatttcttg aatgaaaaac gttctctaca ccgcattcga tatcgtgcca agcccgaagg
     2221 gtgccagcac ccatattctc cacaatttgc gagggttggt caatgggaac tttaatgtcc
     2281 atcttatcac gcccaacgat ggcgtgctcc cccccgaaga tactattgaa ggcgccagac
     2341 tcacgcgcat ttcgcaagac ctttcacaga atttccttgc ccgtgccgtg cattttggta
     2401 agtcagtttt atcgcaccta gccctcaacc ccagctatga tgttgtccac tatcgcaata
     2461 tttgggatgg ctttcacatc acacagaata aaaagaagtt tgggtataaa actatttttg
     2521 aggtgaatgg gctttcttcc attgaactca aatatcatta ccccggtatg gatgccagcc
     2581 tgctcgccaa gatcaaagaa caggaattgg cgactctgca cctgagcgac gccatcatct
     2641 gcccatcaaa cgtgacgcgc gattacatcg cctcgctcgg tcttaaccgc aaactggtga
     2701 ctgttatccc gaacggagtc agcccttcag acttccccgt tacccctttg cccattcgag
     2761 atggacgcga gccggttttg ctttacatcg gtacattagc cgattggcaa ggcttggata
     2821 tcgtcatcaa agccctgccg aaaatccttg agcaacagcc tgtcaaactc cgcatcctcg
     2881 ggcgcggacg ttcccgtcag cgtaagatgt tggcaaagca aattcgcaaa ctgggtttgg
     2941 atgaatatgt tttggtgcaa cctgctgtac cgcatcatga gattccagct gtgatcgcca
     3001 gtgcagatct ttgtgttgca ccactcgggt tgaatgaccg caatgtgact caaggtgctt
     3061 gcccgatcaa agtgcttgag tatatggcgg ctgggcgacc tttactcgcc tcaaatatgc
     3121 ccattgtccg cgaactggtg cgcgaagatg tagacggatt gctcttttca cccaacgacc
     3181 cagacgacct agcgcgacag gcaaatcaat tactgaaaga tatggaacta tccaaacgct
     3241 tggctgagtc tgcggcggga tacgtccgcg agaaatttac atggcacgag tcacagaaga
     3301 aattgttgaa agtgtacgaa aaactaataa agtaacagac cttctttttt gttgaaaatc
     3361 taccccggtt tcagtatata attttcgaat aggaggagca acatgttaga tttcattaaa
     3421 gaagccggtt ttcccgccat tgctttgttg ctcggagggg taatggttgt aattgctatc
     3481 atcagaaaca ttgcccttga aaaagtaaaa atacaactta cttcaagcca gtctattcag
     3541 ttggcagtgg tgggtgtatt gttcattata ggtgggttcg ttttacagta ttttcctcct
     3601 tcttctgcaa atacccctga aaatgttgat gtttattcca cgctttatgc cttacagacc
     3661 caaatggtgc agcctgtaaa cccacaagga caaactaatc agacttctac acctgctggt
     3721 tttgctttgg tttcgccaac cgttaccttg tttccaaacc cagtggcgac cctaacacca
     3781 accttaatcc ccttcacact aactccagat ccttcacaac cattagcaga agtgttttgc
     3841 acgaattttt atagcattat ggtacgtgaa ggacctaatg ataattaccc gatcaaatct
     3901 gttattgaga accccaataa tttgagtaac ccagattgta tgcttttcga ttttcgcatg
     3961 ccagataact catggatacg tattgctgca aatcaggata accccaatta cacccaacat
     4021 gaacttggtt gggtgacttc agatcaattc cgcccgattg attttgataa attgcgagta
     4081 tatatccctg aaaatgtgca agatggtttg tattgtgtca ccagccgata tggtttgaaa
     4141 gtgcggagtt gccccaatga gggttgtcgt gaagttggca acctgtcttt gcaagactgt
     4201 cttttcatgg atgggcgttc ttcagatggt ttatgggtga gagtttcagc cgataaaaat
     4261 gatgagaaat atgcttctta taaaggaaat tgggtgagca cttatttctt agcccccttt
     4321 gaattttcgg ctgggtatca accatatttc cggtattatt ttgaattatt acctactgtt
     4381 gaacaaatcc caaccccgaa tggataacga agaaccaggt cacgcttcaa acgtgacctg
     4441 gttcttttat ggcttgaata tattttctac ttcggcggga attgcttttg ggcgtcctgt
     4501 tttcatatcc ataaaaaccc agtcactttc gccttttact aaagtcttcc catcactctt
     4561 gcggacaaat tcatacatgc gcaacgaccg tactttgcgt aaatcctcta cccaagtgcg
     4621 gacctcaatc tcttcgcctt cgaaggctgg cagaaaatat tcgatgctat gtttacggat
     4681 cacccaggtg gcgtcaggtc cctgggcttc tacgcctccg atggatgcat aatgttcaat
     4741 ggcaatatcc tgcatccatt gcacatagac aacattgttg acgtgtccat tctcgtcaat
     4801 agttgaagag gggattgtga ttgttttgga ataaatagat gaaatcggca ttttttgatt
     4861 ttacagtgac ttcccccaaa cgggggagtt tatttttatt gccccgctga tagaatgaat
     4921 ttaataaaaa tcattttttg gagaaattaa atgggaccaa tcattggtgg cacttcatta
     4981 ataattactg taatttcaat cgcttgtgga tgtttgatta ccattgtcac actggctgga
     5041 actggttttc tgatctacaa ggtatttgga agtacgttca agagcatgtc aacaaatcgc
     5101 aatgttcttc agacgggcgt ttctgctccg gcggtgattc tttctgtgca agacacgggt
     5161 gtgaccatca tgaacaataa tccacaggct cgcatccgtt tgcgtgtgat gccgatgaac
     5221 ggcgaagcat ttgaagcaga agttacccag atcatagggc gttttcaggt gggaatggtc
     5281 gttccgaatg gctcggcaat ggttaaatat gacccgaacg ataaaaccaa agtagcaatt
     5341 gagtccttcg gtatgccatc agccgggcaa taattctaga agaaacaaaa aggcgtggat
     5401 tttatccacg cctttttgtt tttacggtgt acttgtcacg gctggagtgg ttacgtcacc
     5461 tacgacgatg ctgacaaaca atgtatcgcc aaagaagaca ccggtatcgt ctgccatttg
     5521 ccaggcgctt tcgatgacac cattaatgcc actaggcaca acaagttcta cagagagttc
     5581 gcgtttagcg ccggtttgga tggcttcggt aagagtgatg ggagttccac gcatagagtc
     5641 gccgccaacg ttttggaagg tgaaaccagc acgccatgca caaccaccga tgttttgcac
     5701 caaccatgtt ttagtaaatt tttcgccggg tttcatccgt gtcccatctg ggacggtgac
     5761 atcgtcaatg tacaacaggt tgaagcaggg attcgatgtg ggggatggct caggggtgag
     5821 ggaaagcgcc gttgctgtga tgtcaacagg cgtcagtgta ggaaatggtg tgtaggttgg
     5881 agcaagctgg gtcagatttg aggcgtatgt ttccacggct tgggtgcgga tggcatccgt
     5941 atccagcgcg gtagtttgat ctgcctgatt attggcgcac gaaaaaagga atgcgccgat
     6001 gaccaagatc aggctgatga ggatggttct tgatttcatt ctatttttgt ttctctcttg
     6061 ccagtgcaat gccggtggca attgcgccgg tcagttccca gatgggtacg ccgataaatt
     6121 taagcggggc aattccgaga atgacgacag tggtgaatac aaggaaggtc agcccgcgaa
     6181 aagggacggt ccaacggtag aagattttta catcgttcaa tgcggcgagg atgtagtaga
     6241 tgcccatgtt taaggatgcc atggatgccg cggtcatgaa ggtgagggtg tagtctgagc
     6301 tggcacgtgc ggcacgctca acgacttcga agccgagcat ggcaagttga agctccgggc
     6361 ggatgatgcc aaggatgcct agtaaaaatg ccatgacacc aaaaataaac atggtccagc
     6421 cggaagcgtc gcgaattttc atggatacag actcctgtgt gtgcagtgat aacggaaatt
     6481 ataaccgcaa ccattgggca tgataaaatc actaaactat ggctaaacat cttgtgttgg
     6541 tggcaggaaa cattggtgca ggcaaaacta cgttgacaga acgtattggt gcacggcttg
     6601 gctggtggac cggatatgaa tctgtagctg ataacccata tctctctgat ttttatggag
     6661 atatgcggtc gtggtcattt cacttgcaag ttttcttttt ggggcatcgt gctgatcaat
     6721 accttgaggc ggcacgagat gctcgttctg cgattcttga ccgtagtatt tatgaagatg
     6781 ctcatatttt tgcacgtgcc ttgcatcatt taggaaactt gaacgaacgg gattatcttt
     6841 cgtatcgcaa gttgtttgaa ttggtcgtga atggacttcc acgcccagac ctgttgattc
     6901 acctcaaagc gccagttcca gtcttgatgg accgcattcg ccgccgtgca cgtaacatgg
     6961 aaaccggcat ttctgctgaa taccttacct tactcgagtc attctatgac gaatggctcg
     7021 gctctttcga cctttgtcct gtgcttacca tccaaacaga tgacctcgat tatgtaaatt
     7081 atcctcaaca tcttgaccag gttgcacaac gtattcaaga taaattggca ggtagagaaa
     7141 tgatggattt gcgtggagat aacaaataat gaatgaaaag atggatagtt ccgtctttct
     7201 aaagattcca ctgttcaaag acctgcccaa ggcggagttg tattccttgg tcagcgaact
     7261 gccggtagaa aaatatcaag cgggcgatta catcttccat gaaggtgagc cgggcaatag
     7321 tttatacgtg gttcaaaaag gcaagatcga cgtggttctg gcgggcggca cgaacgatga
     7381 aatgcttcta cgcacctgtg gacccggcga atatgtgggt gagatgagtc tcattctcaa
     7441 acaaggcaag cgcacagccg ctgtccgcgt gcgggaagat agccagcttt ggatgatgac
     7501 ccgcgataag ttcaatgaat gtctgcaacg ctggcctcat ttggcatatg ccatggtcga
     7561 gatcatcagc gaacgtttgg actcttcaaa cgaagcagct ttcaacgatc ttgtgaaaaa
     7621 gaatcagatg ctgcaaaaag cctatgatga cttgaaagca gctcaagcac aattgattga
     7681 aaaagagcgc ctcgaacgtg aacttcaatt ggcagctgat attcagctta gtattctccc
     7741 agacgttttg cccgataccg aagagtttgg tttcggcgca cggattttgc ctgctcgcca
     7801 ggtgggcggc gatttttatg atgtcttcca actcaaggat gaccgcattg gggttttgat
     7861 cggtgatgta gctgataaag gtgtgccttc tgcgctgttc atggcacgtg cccatgcgct
     7921 catcatggca gaggcggata ttggtgcgac tcctgccgag gtgatgaact tggtcaattc
     7981 gcacatcacg cgcttgcaaa aatccacgca gtttgtgacc gtgctctatg gcattttgga
     8041 tttgaagact cgcatctttt catatgcacg tgctgggcat gagccgccgt tggtattgca
     8101 taccgatgga agcgtggagc gtattccaca tagccccggc atggcaatcg ggttatggga
     8161 gccggtcacg ttggacgaga agacggtcaa gttatcttct ggcgacacct tgctcttata
     8221 taccgacggc atgaccgact gccgcgaccc acaaggcgac gcatttggct tggaacgcat
     8281 caaggataaa cttggcgaac tttctaagca aagcgcacaa cagatttgca atacattgct
     8341 tgaaacccta caaaatcacc agaatggcgc caagcaagat gacgatgtga ctttggttgc
     8401 cgtaaaagcg aaataaaaaa gacctcccgc aagggaggtc ttcgttttag cgtagagctt
     8461 gttcaaggtc tgcgatcaga tcgtccacgt tttcgatgcc cacggagaga cgtaccaagc
     8521 cggggtccac ttcgagcgtg gaaccttggg tggagagatg cgtcatcgcc gccgggactt
     8581 cgatgagcga ctccacgccg ccgagtgatt ctgccagtgt gaagagtttg gtagcttctg
     8641 cgactttgac cgctgcatca cgcccatctt tcatgatgaa cgagatcatg ccgccgccat
     8701 tcttcatctg tcgcttcgct atctggacct gtgggtgact ctcatgaaat gggtagatca
     8761 gccgtttgac gtttttctgc ttctctaaga acgcgacgat cttctcggcg ttttgggcgt
     8821 gtctatccat gcggatgggc agggtcttga ttccacgcaa cacaaggaag gagtccatcg
     8881 gtccttgcac accgccaatg gcattctgta aataagctaa cttctcgccg agttctttat
     8941 ctttcccgat aatcgcgccg ccgaccacat ccgagtgacc gccgagatat ttcgtcatcg
     9001 aatggacgac caaatccgcg cccagctcaa gtgggcgttg gaggtacggt gtagcgaacg
     9061 tgttatccac aactaataaa ggtttgttcg ggtgattctt caccacttct gccacagcgc
     9121 gaatgtccga cactgccagc aggggattgg tgggggtctc cagccacaca atgcgcgtgg
     9181 a
//
LOCUS       DAOQFT010000018         9179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3550_length_9179_cov_7.759426, whole genome shotgun sequence.
ACCESSION   DAOQFT010000018 DAOQFT010000000
VERSION     DAOQFT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3550_length_9179_cov_7.759426"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLR93_00855"
     CDS             complement(<1..768)
                     /locus_tag="PLR93_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH25533.1"
                     /translation="MTLRTPSTPPSGTIGSYRRRNRGRQNAIVIVAGVLLLGGLALLI
                     YWMTLPGKPINLLLATETPTPTLTYTPTNTNTPTPTFTETPSPTITFTPTFSAPFTYT
                     VQDGDSLAVIAERFNLGEDAIPLILLLNPYVVPTDPAQFPQGVDPTTLNVIPGQVILL
                     PNPDMQLPTATPIPPNLPRGTKIDYVIQSGDSLGAIANAFNSTIDDIMKENNITDPNA
                     LFVGQVLVIPVNMVTPTATRPPTSTPITPGPGTVLPTG"
     gene            complement(912..>1244)
                     /locus_tag="PLR93_00860"
     CDS             complement(912..>1244)
                     /locus_tag="PLR93_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNH25534.1"
                     /translation="GEAQRVKLSKELSRRATGRTLYVLDEPSVGLHAADVHKLIEVMQ
                     RLVDTGNSVLIIEHNLDIIKVADWLIDLGPEGGNRGGELIAEGTPEQVMKVKGSYTGK
                     YLKDHINR"
     assembly_gap    1245..1344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1345..3560)
                     /gene="uvrA"
                     /locus_tag="PLR93_00865"
     CDS             complement(<1345..3560)
                     /gene="uvrA"
                     /locus_tag="PLR93_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNH25535.1"
                     /translation="MSSNKIRVVGARVHNLKNITVEIPRDKFVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFIGQMDKPDVDYIDGLSPAVSIDQKSTSHNPRSTVGT
                     VTEIYDYMRLLFARAGIPHCPVCGREVSKQSAQEIVDKIANLAEGTRILILAPLVRGR
                     KGTYQAVFEEIRKAGFTRARVDGNVYSLDDEIELDRYKQHTIEAVVDRLVISHEGSKE
                     DKKAALTRLTDSIETALKFGEGYITVNLVDKKEDVAYSEHLACPEHGSTVPEIEPRTF
                     SFNTPQGACPDCQGLGSKLEIDPVRIIPDDSVSLNDGAIASMEWGPREEGGYYWSTLV
                     TAAKHYKIDLDAPFRDLTKEQQHVILYGTGDKQLPMKYTNSSGFEVKFSRAFEGVITN
                     LERRYKETNSEYIREKISEYMSDRPCPSCGGKRLNPAALAVTVDNVNIVEANSWPVLQ
                     TLEWVKKISGKGSPLTNKQKTIAERVIREIHERLNFLVNVGLDYLTLNRSAVTLSGGE
                     AQRIRLATQVGSRLVGVLYVLDEPSIGLHPRDNTRLLETLKGLRDLGNTVLVVEHDDE
                     TILESDWVIDLGPAAGEHGGQIIAEGTPKQILAHPKSLTGLYLSGRKQVEIPKKRREG
                     NGKLLKIVNATANNLKGIDVNIPLGKLVCITGVSGSGKSTLMSDILYNELAAKLMGAR
                     TQSGEHKTIEGIEHLDKIINIDQSPIGRTPRSNPGTYTGLFDEIRKLFAELPESKVR"
     gene            3764..6274
                     /locus_tag="PLR93_00870"
     CDS             3764..6274
                     /locus_tag="PLR93_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNH25536.1"
                     /translation="MSTKNKVSTEQSSISPELASTIPSNLPVLRTKLIGREAEQKTVL
                     DLLNQTDIRLVTLVGFGGAGKTTLALHTSHALFEKFPGGLFFIDLTSIYEPALILPTI
                     AATLGLQEEPSREIADTLRDFLSNRSILFVLDNFEQLISGANIIASFLDANPHIRLLV
                     TSREALHLRGEHTLSLAPLESADAMQVFTQYAQTLNPHFRLTEDNTPAITELCNKLDG
                     LPLAIELAAMRTRMFTPQALLARLTSDLETGSPLLATLTSGPRDMPERQRTLRNMVAW
                     SYNLLTDKEKHMLQAAALFRSGFGIGSLAQVTAVPENEAEHILASLVDKNLIQPFHGD
                     EVRFHLLESIREFALEQALKQEDVNTLTEKFVGYYLALARDAEQGFRSPEQVQWLDLL
                     DDELANIQTALRFALNDGYAGSFFQSGYEMLIHLQRYWLLRAHYAVGVSWLEKAKAII
                     DRQVAISSGVISNDLLYQKSKIYGFLGYAYWIMGRYAIAKDLHEISLTAARTLNREDL
                     LWESLNNSAVNLEYMGEFEAAMTYYAQALEIIKRTGDRWQEMRLLINLGNSYVGIEEY
                     ETGRTYLEDALLLTMELKDDYYQAACLQGLGYLELRLRRHKEAEENFLKSLSLFERMD
                     VPFIYTWALVSLARVLGETSKHKETAQVVLKCVEMLETQKDRNLYRTMFETLVAFCIS
                     LNKNDMAARFIGFIEQNYAGESEVILPADKAIFDAKTQQVGSMMDQREYVLEKETGAK
                     GSASLLFQLASGICDEILNDTHSKPVLLELLTERELSVLRLLSQGRTNEEISKELVVV
                     LKTVEKHVANILRKLGAKNRTEAAIWYLENTSNLRQND"
     gene            complement(6320..7222)
                     /locus_tag="PLR93_00875"
     CDS             complement(6320..7222)
                     /locus_tag="PLR93_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25537.1"
                     /translation="MKRQTLVVILNLALTFSLFGSVSIAHAQTTCTDATGATSPCPPS
                     GGDSGSNEEDDNGSNQPGGGNPNPPTDITATPSPTPSPEPTSTPLPLQAQPTLDPTKN
                     APVFEVTPTPTAEPVITSSGNDGATEGENDWEDVCTFDLCLGLTAACYWDGGTPEIVE
                     DGSGNTGVHCTIPDDDDGAAAPFPWGPVGIVGIVVLLMVMLLPAVQKFKNKPDTPQGR
                     SWDTFGDIKGDNVAPTSTPQTREHILLAKQQDGNTSGASKPKPKPKPAPQPTPPPAQP
                     KPPSSGTPGDFNSDGTVDGNDFIP"
     gene            7421..7984
                     /locus_tag="PLR93_00880"
     CDS             7421..7984
                     /locus_tag="PLR93_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH25538.1"
                     /translation="MEYHEEDVLMRASQGDKDAFGELYERYAERIFNYVYYRTGNQHD
                     AEDLTARVFQRAMNHIRNYTDRGVPFSAWLYRIAHNLVANWHRDRSRKQEIPIDDLPI
                     LPTKGDHPERNLVRSQEQDALLKMIRKLPPERQNLLILKFVENMSNAEIGKIMGRSEG
                     AVKSLYHRTLLALRDQLEDQNLNLEGE"
     gene            7987..8835
                     /locus_tag="PLR93_00885"
     CDS             7987..8835
                     /locus_tag="PLR93_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNH25539.1"
                     /translation="MLRIVTDGAVDLPTEWYKEYDIHRIPINVHFGEEKTFIQDVELD
                     QDGFYKLVDGKTLPHPKTSQPSPHQFAEFYKKIAQPGDTILSIHITSKLSGTYASSVA
                     AAEELKGTYTVVPVDTLCGTMGTAFMCRSARQMERAGKSVDEIKTFIEGVRGKTHVIL
                     TLETLEYAKRSGRVGTLSAALASVLNVKPIAMLKDGIVDMVDRVRTRKGAIDRVLDLA
                     KENVGDSPVHIGVVHARDEASAKSLLEEAQKRFNVKESFLTDLSISLVINFGPGTVGL
                     VLYPAE"
     gene            8845..9114
                     /locus_tag="PLR93_00890"
     CDS             8845..9114
                     /locus_tag="PLR93_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25540.1"
                     /translation="MSENQTFTSQDTDLQFLESHLSRTLKRVPPPNGLVERLRGRVQM
                     PTRSEIRLRFSDWRRLFVVFGGVMSGMLVLVTLARAFYYLVGRKA"
BASE COUNT         2245 a         2050 c         2346 g         2438 t
ORIGIN      
        1 gccggttggt aaaacggttc ctggtcccgg tgtgatgggg gtgcttgtgg gcgggcgggt
       61 ggcggtaggt gtcaccatgt ttacaggaat taccaaaacc tgtccaacaa aaagggcatt
      121 ggggtctgta atattgtttt ccttcataat atcgtcaata gtgctattga aggcattggc
      181 aatggcacct aaagagtcac cagattggat gacgtaatct attttcgtgc cgcgcggcaa
      241 gttaggtgga atgggagtgg ctgtaggcaa ttgcatatct gggtttggaa gcaaaatcac
      301 ctgcccagga atcacgttta gggtagttgg gtcaacgcct tgtgggaatt gagccggatc
      361 ggttggaaca acatacggat tcagcaaaag aatcagcgga atggcatcct cgccaagatt
      421 gaatctttct gcgatcactg ccagagagtc tccatcttgt actgtatatg taaatggagc
      481 tgagaaggtc ggtgtgaagg taatggtggg cgaaggagtt tctgtgaatg ttggtgtagg
      541 tgtatttgta tttgttggcg tataggtgag tgttggggta ggggtttcag ttgccaacaa
      601 caggttgatg ggtttgccgg ggagagtcat ccaatagatc agcaatgcga gacctcccaa
      661 caggagaacg ccggccacaa taacgatggc gttctgcctg ccgcggtttc gtctgcggta
      721 ggagccaatg gttccagaag gaggggtgct tggagttctg agagtcattt aatcaatcct
      781 tttgcacgtc ggtctaaatg tattttccaa ccaattgcag tgcaaaataa ttctacctga
      841 aaaaggggaa tcgcgtgtgc atggcacaaa aaaacaggct gacattgcca gcctgttttc
      901 aaaatgctct attagcggtt tatatggtct ttcaagtatt tgcccgtata agagcctttc
      961 accttcataa cttgttctgg tgtgccttca gcgatcaact cgccgccacg gttaccgcct
     1021 tctggaccaa gatcaatgag ccaatctgct actttgataa tatccagatt gtgctcgatg
     1081 ataagcactg agttgcccgt gtcaacgagc cgctgcatca cttcaatcaa cttgtgaacg
     1141 tctgctgcat gtaaaccgac agaaggttca tccaatacgt aaagtgtacg tcccgttgcc
     1201 cggcgagaaa gttctttcga gagtttcacg cgctgtgctt caccnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnncgcact ttagactcag gaagttctgc aaacaactta
     1381 cgaatttcat caaacaagcc agtgtatgta ccgggattcg aacgtggcgt gcgtccgata
     1441 ggggattggt cgatgttaat aatcttatcg agatgttcaa ttccttcaat cgttttatgc
     1501 tcgccagatt gtgtccgcgc acccatgagt tttgccgcaa gttcgttata caggatgtcg
     1561 ctcatcagtg tggacttgcc cgaacctgag acgcctgtaa tacaaactaa tttcccaaga
     1621 gggatattga catcaatgcc cttcaaatta tttgctgttg cattaacaat tttgagcaac
     1681 ttgccattgc cttcgcgcct ctttttcggg atctcgacct gtttgcgccc cgagagatag
     1741 agtccagtca gtgactttgg gtgcgccaaa atctgcttcg gtgtgccttc tgcaataatt
     1801 tgtccaccgt gttcgcccgc agcaggaccc aagtcgatga cccaatctga ttcaagaatg
     1861 gtctcatcat catgttcaac gacaagaacc gtatttccca gatcccttaa ccctttaagc
     1921 gtttcaagca gacgggtgtt atcacgcggg tgcaaaccaa tggatggttc atctaaaacg
     1981 tagagaacgc ctacgagacg agaaccaacc tgagttgcaa ggcggatgcg ttgcgcttct
     2041 ccgccagaga gagtcacagc agagcggttc aaggtcaaat aatccaaacc aacgttgacg
     2101 aggaagttca agcgctcatg aatttcacgg atgacgcgtt cggcaatggt cttttgttta
     2161 ttggtgagtg gtgagccttt gccagaaatt tttttgaccc attcaagcgt ttgtagcaca
     2221 ggccatgaat tcgcttccac aatgttcaca ttatctacag tcacggcaag agcagcgggg
     2281 ttgagacgtt tgcctccgca cgatgggcat ggacgatcgg acatgtattc tgagattttt
     2341 tcgcggatgt attccgaatt ggtctctttg taacggcgtt cgaggttggt gataacccct
     2401 tcaaacgcac ggctgaactt gacttcaaat ccgctcgagt tggtgtattt cattggaagt
     2461 tgtttgtcgc ctgtgccgta gagaatgaca tgttgttgtt cttttgtcag atctctaaat
     2521 ggagcatcaa gatcaatctt gtaatgctta gcggcggtga caagtgtgct ccagtagtaa
     2581 ccgccttcct cacgcggacc ccactccata ctggcaattg ctccatcgtt cagtgacaca
     2641 ctatcatcgg ggatgatgcg aacaggatca atttccaact tggaaccaag cccttggcag
     2701 tcagggcagg cgccttgagg cgtgttaaag gaaaaggtac gcggttcaat ttcgggcacg
     2761 gttgagccgt gttctgggca ggcgagatgc tccgagtagg cgacatcttc ttttttatcg
     2821 acaaggttga cggtgatata tccttcacca aatttgagag ctgtttcaat cgagtctgtt
     2881 agacgggtga gcgcagcttt cttatcctct tttgaacctt catgcgaaat gacgagacga
     2941 tctactacgg cttcaatggt gtgctgtttg taacggtcaa gttcgatctc atcatccagc
     3001 gagtagacgt ttccatcgac gcgggcacgg gtaaaacctg ctttacggat ttcttcaaag
     3061 acggcttggt atgtaccttt acgtccgcgc actagtggag caaggatgag gatgcgtgta
     3121 ccttcagcca gattagcgat cttatccaca atttcttgtg ccgactgttt gctcacttcg
     3181 cgtccgcaga cggggcagtg cggaatgccc gcgcgcgcaa acaagagacg catgtaatcg
     3241 tatatctcgg tcactgtgcc aacggtagaa cgcgggttgt ggctggtgga tttctggtcg
     3301 atggaaacgg caggggagag cccatcgata taatcgacat cgggcttgtc catctgaccg
     3361 atgaactggc gtgcatatgc cgaaagcgac tcgacataac gcctctgacc ttcggcgaag
     3421 atggtatcaa acgccagcga cgatttgccc gaacccgaaa gcccagtgat gacgacgaac
     3481 ttatcgcgtg gaatttccac ggtgatattt ttcaaattat ggacgcgtgc gcccacgacg
     3541 cggattttat tggatgacat gagactcctg tttgggagaa ggtattatag cacaggtgtt
     3601 ctggtaatcg tagtccctgg caaaaagaaa aaatgattat accaatcagg gtgaacacgc
     3661 cttctcattt catgtaaatt gtggtgtctt acaaaatcat actttttggg gagtaagttg
     3721 tgccataaaa aagagaccaa cccgttataa ttttttactg accatgtcta caaaaaacaa
     3781 agtatcgaca gagcaatcaa gcatctcgcc tgagcttgct tctacgatcc ctagtaactt
     3841 gcctgtcttg cgcacaaagt tgatcggacg tgaagcagag cagaagactg tcttggatct
     3901 cctaaaccaa accgatatac gtcttgtgac ccttgttggg tttggcggtg cgggcaaaac
     3961 cacgttggca ttgcacacct cacatgctct gtttgagaaa tttcccggcg ggctgttctt
     4021 tattgacctg acttccattt acgaacccgc tttgattttg ccgaccattg ccgcaacact
     4081 gggtttgcaa gaagagccca gccgcgagat cgccgacacc ctgcgcgatt tcctgtccaa
     4141 tcgttctatc ctttttgtgt tggataattt tgaacagctc attagcggcg caaatatcat
     4201 tgccagtttt ctcgatgcca acccgcacat tcgtttgctc gtcaccagcc gtgaggcgtt
     4261 gcacttgcgc ggtgagcaca ccctgtcgct tgctccgctc gaatcggcag atgccatgca
     4321 ggtcttcact caatatgcac aaaccctcaa cccgcatttt cgattaaccg aagataacac
     4381 ccctgccatt accgaacttt gtaacaaact ggatgggctt ccgcttgcca tcgaacttgc
     4441 cgccatgcgc acgcggatgt tcaccccgca agccctgctc gcccgcctca catcggatct
     4501 cgaaactggt tcgccacttt tagccactct tacatccggt ccgcgtgaca tgcctgaacg
     4561 tcaacgcacc ctgcggaaca tggttgcctg gagttacaac ctgctcacag acaaagagaa
     4621 gcacatgctg caagcggcgg cgctcttccg ctcgggcttt ggcataggct cactggcgca
     4681 ggtaacagcc gtccctgaga atgaagcgga gcacatcctc gcctcattgg tggataaaaa
     4741 tcttatccag cctttccatg gagatgaggt tcgttttcat ctcttggaaa gtatccgcga
     4801 gtttgcgctg gagcaggctt tgaagcaaga agacgtgaat accctcacag aaaagtttgt
     4861 tgggtattac cttgcattgg cgcgagatgc cgagcagggt tttcgtagcc ctgaacaggt
     4921 gcaatggctg gatctgcttg atgacgaact tgccaatatc caaaccgcat tgaggtttgc
     4981 acttaatgat ggatatgcag gttcgttctt tcagagtggg tatgaaatgt tgatacactt
     5041 gcagcggtat tggctgttgc gtgctcatta tgcagtcggt gtatcctggc tggaaaaggc
     5101 gaaagccatc atcgataggc aggttgccat ttcatcgggg gttatctcaa atgatttgct
     5161 ttatcagaaa tcgaagatct atggcttttt ggggtatgca tattggatca tggggcgcta
     5221 cgcgattgca aaagatttac atgaaatatc actgacggct gcgcgtacct tgaatagaga
     5281 agatctatta tgggaaagtc tcaataactc tgctgtcaat ctggaatata tgggtgaatt
     5341 tgaagcggcg atgacatatt atgcacaggc gcttgagatc attaagcgga cgggtgatcg
     5401 ttggcaagaa atgcgtttac tcattaatct tggaaattca tacgttggta ttgaagaata
     5461 tgaaactggg cgtacctatc ttgaggatgc cctgcttttg acaatggagt tgaaagatga
     5521 ctattatcaa gccgcttgtt tgcaagggct tggttatctt gaacttcggt tgcggcgaca
     5581 caaggaggcg gaagaaaatt ttttgaaatc gctatccttg ttcgaacgca tggatgtgcc
     5641 gtttatttac acatgggcat tggtcagttt ggcgcgtgtt ctgggcgaaa ccagcaaaca
     5701 caaggaaacc gcgcaggtcg ttttaaaatg tgtagagatg ctggaaacac aaaaagaccg
     5761 gaacctatac cgaaccatgt tcgaaaccct ggttgctttt tgtatttcac tgaataaaaa
     5821 tgacatggct gcccgcttta ttgggtttat tgagcagaat tatgcggggg aaagcgaggt
     5881 tatcctaccg gcagacaagg cgatctttga tgcaaagacg caacaggttg gcagcatgat
     5941 ggatcaacgt gagtatgtgt tggagaaaga aaccggcgcc aaaggcagcg cttcactttt
     6001 attccaacta gcctctggta tctgtgatga aatcttaaac gatacacact caaaacctgt
     6061 tttactggaa ttactcactg aacgcgagtt gagtgtactg cgtttgcttt cgcagggcag
     6121 gacaaacgaa gagatttcaa aggaactggt cgttgtactt aagaccgttg agaaacatgt
     6181 ggcaaatatt ctcagaaagt tgggcgccaa gaaccgcacc gaagcggcaa tttggtatct
     6241 ggaaaacaca agcaacctac gtcaaaatga ctgacgcggg ttgcttgtgt gaaattaata
     6301 ggtttgagat tcgcaattat taagggatga aatcatttcc gtctacggta ccatcagaat
     6361 taaaatctcc gggagttcca ctgctgggtg gcttgggttg agcagggggc ggagttggtt
     6421 gtggcgcggg tttgggcttg ggtttgggtt tggaagcgcc tgatgtgttg ccatcttgtt
     6481 gctttgccaa aagaatatgc tcgcgagttt gtggcgttga ggtgggtgca acattgtcac
     6541 ccttaatatc tccgaaagta tcccaagacc ttccctgcgg agtatctggt ttatttttga
     6601 acttttgaac tgcgggcagg agcatgacca taagcaaaac aacaatccca acaataccaa
     6661 ccggtcccca agggaaggga gccgcagcgc catcgtcgtc atctggaatg gtgcaatgaa
     6721 cgccagtgtt gcctgacccg tcttctacga tctcgggtgt gccgccatcc caatagcagg
     6781 ctgcggtcaa gcccaggcaa aggtcgaagg tgcagacatc ctcccagtca ttttctccct
     6841 cggttgcgcc atcattgcca gaggaagtga ttacaggctc ggcggtgggt gtgggagtca
     6901 cttcaaagac aggtgcattt ttagtggggt ctagtgttgg ttgtgcttga agtggtaggg
     6961 gcgtactcgt cggctcggga gatggagtgg ggctgggtgt agccgtaata tctgtgggtg
     7021 ggttggggtt tccaccacct ggctggttgc tgccattgtc gtcctcttca ttagagccgc
     7081 tatcaccgcc agagggcggg caggggctgg tggctccggt ggcatcggta caggtggttt
     7141 gggcatgggc aatggataca gacccgaaca aagagaaggt cagcgccaga ttcaaaatta
     7201 caactaacgt ttgtcttttc ataaaaagct cctttgcatt ggttttaaca ataagtgttt
     7261 gcattatccc caaagcggtt ctgcttgtcg gtagggttaa ccctactctt ccccctgttt
     7321 tttgatagga aattggtcat gttatactga gtgcaacttc tcaaatccgg cggtgtttta
     7381 ttattcaagt tccgccggta aggtttctca cttgtcggat atggaatatc acgaagaaga
     7441 tgtgttaatg cgcgcctcac aaggcgacaa ggatgcattt ggcgagttgt atgaacgcta
     7501 tgcagagcgt atcttcaact atgtgtacta tcgcacgggg aaccaacacg atgcagaaga
     7561 tttaacagca cgtgtatttc agcgggcgat gaatcacatt cggaattaca ccgatcgggg
     7621 tgtgccattt tcggcgtggt tatatcgtat tgcgcacaat ctggttgcca attggcaccg
     7681 tgaccgcagc cgaaaacagg aaatccccat cgatgacttg cctatcctgc caacgaaggg
     7741 tgaccaccct gagaggaatt tagtccgctc gcaggagcag gatgcactac taaaaatgat
     7801 ccgcaaatta ccgcccgaaa ggcagaattt gcttattttg aaatttgttg aaaatatgtc
     7861 caacgctgag atcggcaaga ttatgggtag aagcgaagga gccgtgaaga gcctgtacca
     7921 ccgcactctt ctggcgcttc gagaccaact ggaagatcaa aatcttaatc ttgaaggaga
     7981 gtaagcatgt taagaattgt gacagacggc gctgtggacc ttccaacgga gtggtacaag
     8041 gaatacgata ttcatcgcat tcccattaat gtacattttg gtgaagagaa aaccttcatt
     8101 caagatgttg aattggatca agatgggttt tataagttag tagatggaaa aacccttcct
     8161 catccaaaaa cttcgcagcc ttcgcctcac cagtttgcgg agttctataa gaagattgct
     8221 caacctggcg atacgatcct ttccattcat atcaccagca agctctcagg gacatatgct
     8281 tccagtgtgg ctgctgctga ggaattaaaa ggaacctaca cagttgtacc agtcgatacc
     8341 ttgtgtggaa cgatgggcac tgcctttatg tgccgttctg cccgccaaat ggagcgtgct
     8401 ggcaagagtg tagatgaaat aaagactttt attgaaggtg tgcgtggtaa gacccacgtc
     8461 attctcacgc ttgagacgct tgaatatgcc aaacgtagcg gacgtgtagg cacactttca
     8521 gcagcacttg cttcggtgtt gaatgtcaaa ccgattgcga tgttgaaaga tggcattgtg
     8581 gatatggtcg atcgtgtgcg cactcgtaaa ggtgccattg atcgcgtgct cgaccttgca
     8641 aaagaaaatg tgggcgatag cccggttcat atcggtgttg tccatgcgcg tgatgaggct
     8701 tctgccaagt ctctgttaga ggaagcgcag aaacgcttca acgtcaaaga aagctttttg
     8761 accgaccttt ccatctccct tgtaatcaat ttcggacccg gaacggttgg attggtgtta
     8821 taccctgctg agtaattaaa ttctatgagc gaaaaccaga cattcacttc ccaagataca
     8881 gacctgcaat ttcttgaatc gcatctctcg cggaccttga aacgggtgcc gcctccgaac
     8941 ggcttggtcg aacgcctgcg cggacgcgtg caaatgccca cacgttctga aattcgattg
     9001 cgtttttcag attggcgtag attgttcgtt gtgtttggtg gcgttatgtc tggtatgttg
     9061 gtcttggtta cactggcgcg tgcgttttat tatctggttg ggcgcaaagc gtaacttctg
     9121 cattttctac aaaaaactcc tgaaagatat ctttcaggag tttttctttt aatttttca
//
LOCUS       DAOQFT010000019         8766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3782_length_8766_cov_6.437952, whole genome shotgun sequence.
ACCESSION   DAOQFT010000019 DAOQFT010000000
VERSION     DAOQFT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3782_length_8766_cov_6.437952"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PLR93_00895"
     CDS             complement(<1..1277)
                     /locus_tag="PLR93_00895"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNH25541.1"
                     /translation="MTQEDPRILELRRVRAKAKEGGGEERNAKQRAKGKMTARERIEA
                     LLDPGTFNEIEPFITHQGDELGLLKEKFYGDGVITGYGQINGRTVYLYSQDFTIYGGT
                     LSEMQSHKICRVMDLAVRNGVPFISLIDSGGARIQEGVRSMGGYAEIFRRNAQYSGVV
                     PQVSVMLGPCAGGAAYSPALTDLVIMVEKQSFMFLTGPDVIKAVTGEVIDAESLGGSV
                     VHNSVSGVAHLSVTSEMAALEMARKFLSYLPSNNVENPPYVQPSDALSRMDEDLNHII
                     PLEANEPYVMHQVIEKIVDKNSFLEIQPDWARNAIVGLARIGGHSVGIVSQEPSIMAG
                     VIDIDAADKMTRFVRMCDCFNIPLVTFVDSPGFLPGIAQEHGGIIRHGAKLLYAYSEA
                     TVPKICIITRKAYGGAYVVMSSKYLGTDVTYAWP"
     gene            complement(1479..2669)
                     /locus_tag="PLR93_00900"
     CDS             complement(1479..2669)
                     /locus_tag="PLR93_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNH25542.1"
                     /translation="MSLIQEKTDQAVQILKEQDIDLWLTFVRETSGVRDPVLSFIIGE
                     ADLTWPSALIMTKHGDKIAIVGNLETEALQRLDVFTQIIGYDTGVSGVLRETISKLNP
                     NQIAVNMSRNNVHADGLTHAMYEFLVEYLSGTPYPERIISAEPVINALRGRKTKAELA
                     RIQRAVEITDEIYKKTFAFIKVGMTEIQIADYMQALMKEYGVGYAWPRENNPAVNSGP
                     DKVVGHNGPTDIKVEPGHIIHFDFGVKHEGYCSDIQRIAYVLREGETEAPVEVQRGFI
                     TVRTAIEKSREAMKAGVIGNDIDTISREIIVDSGYPEYMYALGHQLGRVAHDGGALLG
                     PRWEKYGDSPNQKLEVGQVFTIEPGLAVPHYGYVGLEEDVVMTEEGALYVGDPQREIV
                     LIGR"
     gene            complement(2685..3956)
                     /locus_tag="PLR93_00905"
     CDS             complement(2685..3956)
                     /locus_tag="PLR93_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH25543.1"
                     /translation="MLSPLEQAIRKDLKHNVTAGLWDGSLFGVALGFASFGTILPLFV
                     ATMTQSATLIGLVPAIHSAGWLFPQLFTASHVSRLRQLKRTVLLNTIHERVPFLGFAA
                     VALLLPIVGVNAGLLLTFLLLIWQGLGGGFTANAWTSMVSKIIPPESRGTFFGMQAGL
                     ANLFISGSAIAAGYLLNALASPWDFAVCFFVASIFFALSWFALAATREPEDTEKFIPE
                     EKSHFWDDAKKVLSRDTNFNWFLIARILSQFATMGFSFYIIYALREFNMTEVVAGYLT
                     ATLTISQTIANIGMGWIGDRVGHRVMLIIGAFSAFLSALLAWNAASIAWFYPIFILTG
                     LANVSMWTISMTMTVDFGNETDRPIYIGLSQTLTAPATIIAPILGGWIADSAGFVPTF
                     TISTILSLVMVGILIFLVKDPRTSKLVNLQS"
     gene            4094..4702
                     /locus_tag="PLR93_00910"
     CDS             4094..4702
                     /locus_tag="PLR93_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH25544.1"
                     /translation="MAEETHTKGERTRIQIEDAAIELFIEQGYHATSMRQIADRADLA
                     LGGIYNHFKSKDEIFEAIIVDKHPYKRLLPLILEAEGHTMEDFLGHAARVVIKELTSQ
                     PYYIKLMLIEIAEFNGVHGAVLIKEIGPKLLPVFERIIKTRKNLRLTNPAMLMRTFIG
                     MVVSYMITEMLISNSMLNKMMPKNPIDAYVDIYLHGIVKSEA"
     gene            4707..5837
                     /locus_tag="PLR93_00915"
     CDS             4707..5837
                     /locus_tag="PLR93_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25545.1"
                     /translation="MFNTRIVSIVRKEFVQIFRDPRSLALIIVMPIIQLFLLGYAATT
                     DVKNVPIAVLDQSQSAQSRELLDAFRAADYFRIDYVVGSTEEYQLLIESGKIRAALVI
                     PPDYDVQVLDGKAQVLFVLDGSDGSVGATALSTARLIGQSYSTKVQTSQFALRGSGSL
                     PTQPVEVRTQVWYNPDFQSAYFMIPGVIGMILSFITTILTATTIVRERERGTIEQLIV
                     TPIRSWELIVGKLLPYVILAFVETFEILIVGHLAFDVPVRGSLLLITLASGLFLMSSL
                     GIGLFASTIANTQQEAMLSVMMFNLPMIFLSGFFFPVQAMPQFLQFVSLAIPLRYYLV
                     VIRALMLKGVGFAAIQSEIISLTIFGIVIMGIAALRFRKRLD"
     gene            5879..7189
                     /locus_tag="PLR93_00920"
     CDS             5879..7189
                     /locus_tag="PLR93_00920"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH25546.1"
                     /translation="MQNKLPPLPVRIVIAVIVLGTLAYFGFQSLKDDTNGSLTASGTI
                     EATIVNVAPETAGKVTDVRADEGESVTKGQSLLSLDASLLTAQQAVASAAVDSATAAL
                     AAAQTKYDQTLEAALAAQAAQRSKDWQASAPSEFDQPNWYIEQDQQVSSAQAELDAAK
                     AEIDAANENLDRVITDLKNSEFLKTEQELADARAAFFIAQQVKDQASSASDNSGLTDA
                     AQDYYDDAVTRLNDAEEAYNDLMSSDEADDVRYARGQVLVAQQRYDAAYARLLTLQTG
                     ENSPAVIAAGKELDKAKSALAQAEASLALVQAQIAKLEVHAPMDGVILTRNVEPGEFV
                     QPGAAALTIGDLSDLTITVYVPENRYGEISLGQTAEVTVDSFPGVTFTATVIQIANKA
                     EFTPRNVQTVEGRSATMFAIKLSVQDAEGRLKPGMPADVIFISK"
     gene            7256..8218
                     /locus_tag="PLR93_00925"
     CDS             7256..8218
                     /locus_tag="PLR93_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25547.1"
                     /translation="MDDYLVNATGLKKSYGANHAVSGVSLHIPPGEIYGLVGADGAGK
                     TTTMRLLVGALLPDAGEISVCGYDVLKQTERARSTIGYLSQRFSMYEDLTVLENIRFF
                     AEIRGLSSTEWLPRSLEILDFVGLEKFKDRRAGQLSGGMKQKLGLASALVTRPRLLLL
                     DEPTTGVDPVTRQDFWQLVIKLVGSREGDGVSVIISTPYMDEASRCHRVGFMKNGRII
                     TEDTPSNLRSLLNGRILELRGSPLNTLRHVAHTDVDVEDVQAFGDRLHIRVRDDKANQ
                     VLRRLKSSIKAAGGHVVELRMVPPALEDVFIALSEDKAISAVSV"
     gene            8234..8668
                     /locus_tag="PLR93_00930"
     CDS             8234..8668
                     /locus_tag="PLR93_00930"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH25548.1"
                     /translation="MINIDLGVLIDRPVKDVFAFVSNPNNMTKWNSAVINLEPLTPGT
                     VGVGAKFKTTGEAMGRRIEGEMQITAYEPDTKCGFQVTAGPMQVNLTMIFKTVGTGTK
                     VSLNAQGNPGGLFKLAEGVMAGQVKSMMEGNLSRLKSALEGS"
BASE COUNT         2161 a         2140 c         2191 g         2274 t
ORIGIN      
        1 ggccaggcgt aggtgacatc ggtgcccaaa tacttgctag acatcacgac ataggcgccg
       61 ccataggctt tgcgggtgat gatacaaatc tttggcacag tcgcctcaga gtatgcataa
      121 agcaatttcg caccgtgacg aataatgccg ccatgctctt gcgcaatgcc gggtaagaag
      181 cccggtgaat ctacgaaggt tacgagcgga atgttgaaac aatcgcacat gcggacgaag
      241 cgtgtcatct tatcggcagc gtcaatatcg atcacgcctg ccataatgga cggctcttgc
      301 gaaacgatgc ccacactatg tccaccaatg cgtgccagcc caacaatggc gttacgtgcc
      361 caatcgggtt gaatttcaag gaaagagttt ttatcaacga tcttctcgat aacttggtgc
      421 atcacatagg gttcattcgc ttccaatgga atgatgtgat taaggtcttc gtccatgcga
      481 gaaagtgcat cgctgggttg aacatacggc gggttttcta cgttattcga cggcaaatac
      541 gaaaggaatt tgcgtgccat ttccaatgct gccatttcac tagtgacact caaatgtgcc
      601 acgccgctca ccgagttatg aaccaccgaa ccgcccagag actcagcatc gatcacttca
      661 ccagttacgg ctttgatgac atctggaccg gtcaggaaca tgaacgattg tttttccacc
      721 ataatgacca ggtcggttag agcgggcgag taagcagcgc caccggcaca tggaccgagc
      781 atcacgctga cttgtggcac aacacccgaa tattgagcat tacgccgaaa gatttcagca
      841 tatccgccca ttgaacgcac accttcttga atgcgcgccc cacctgagtc gatcaacgaa
      901 atgaatggca cgccattacg aactgccaga tccatcacac ggcaaatttt gtgcgattgc
      961 atctcgctaa gtgtgccacc ataaattgta aaatcttgtg aatataagta aaccgtccgc
     1021 ccgttaattt gcccataccc tgtgatcaca ccatccccgt aaaatttctc tttcagtaat
     1081 cccagctcat cgccttggtg ggtgatgaac ggttcgatct cattgaaggt gcctgggtca
     1141 agcaaggctt caatgcgttc gcgtgccgtc atcttccctt tggcacgctg tttggcattg
     1201 cgttcttcgc cgccaccttc ctttgctttc gcacgaactc gccgtaattc caaaattcta
     1261 gggtcttctt gagtcatgtt aagtccttta ggtgaaaatt ttcacaatca aaagtataac
     1321 attggatgtc agacaagcca gtgatggagg tcatattgga aataggacaa tccgcctcct
     1381 catttgattg ggggctggtt ctgttttatg agctttttcc accctgcaaa ttgttgctac
     1441 aaaaagagcg tggacaatta tcgtccacgc tctttcgtct atcgcccgat caaaacgatt
     1501 tctctctgcg ggtcaccaac atagagagcg ccttcttccg tcatcaccac atcttcttcc
     1561 aagccaacat acccgtagtg gggcacagca agacccggct caatagtgaa aacctgacct
     1621 acttccagtt tttgattggg cgagtctcca tatttttccc aacgcggacc caacaatgcg
     1681 ccaccatcat gtgctacacg cccaagctga tgcccaagcg catacatata ttcggggtat
     1741 cccgagtcca cgatgatctc gcgtgagatg gtatcgatgt cgttcccaat cactccagct
     1801 ttcatcgcct cacgcgattt ctctatcgcc gtgcggacgg tgataaaccc gcgctgaact
     1861 tctactggcg cttcagtttc gccttcgcgc aacacataag caatgcgttg aatgtcggaa
     1921 caatagcctt cgtgcttcac gccaaagtca aagtggatga tatgacctgg ctcgaccttg
     1981 atgtcagtcg gtccattatg cccaaccact ttatcgggtc ctgaattgac cgccgggtta
     2041 ttctcgcggg gccaggcata gccaacgcca tattctttca tcaaggcttg catgtaatct
     2101 gcgatctgaa tttcggtcat gccaactttg ataaaagcaa aggtcttctt gtagatttca
     2161 tctgtgattt ctacggctct ttgaatgcgg gcaagttcgg ctttggtctt gcgtccacgc
     2221 agggcgttga tgaccggctc tgcgctgata atgcgttcgg ggtatggtgt gcctgagaga
     2281 tactccacca gaaattcata catggcatgt gtcaacccat cggcatggac gttgtttctg
     2341 gacatattga ccgcgatttg attcgggttg agttttgaaa tggtctcacg cagcacaccg
     2401 ctgacgcccg tatcgtaccc aatgatttga gtaaacacgt ccaagcgctg cagtgcttcg
     2461 gtttcgaggt tgccgacaat cgcaattttg tctccgtgtt tggtcataat gagcgcggag
     2521 ggccaagtca gatcagcttc accgatgatg aaactcaaca ccgggtctcg gactccgctt
     2581 gtctcgcgga cgaaggtgag ccagaggtca atatcttgct ctttgagaat ttgaacggct
     2641 tggtcggtct tttcttgtat gagggacata gatgctcctt ggatttatga ttgtaaattt
     2701 acgagtttcg acgtacgcgg gtctttgacg aggaaaatca aaatgcctac catcaccaat
     2761 gaaaggatgg ttgagatcgt aaatgtaggt acaaacccgg cgctatctgc gatccagccg
     2821 ccgaggatgg gggcaataat ggttgctggg gcagtgagtg tttgtgataa gccaatgtaa
     2881 attgggcggt cagtctcatt gccgaagtct acggtcatgg tcatgctgat ggtccacatg
     2941 gagacatttg ccaagccagt gagaatgaag atggggtaaa accatgcaat tgaagcggca
     3001 ttccacgcaa gtaaagcgct taggaatgca gagaatgcgc cgatgataag catgacgcga
     3061 tgcccaaccc ggtcaccgat ccagcccatg ccaatgttgg caatcgtttg tgaaatggta
     3121 agggttgcgg tgagatatcc cgccactact tcggtcatat tgaattcgcg tagggcgtag
     3181 ataatgtaga acgagaatcc catggtcgca aattgtgaga ggatgcgtgc gatcaagaac
     3241 cagttgaagt tggtatcgcg gctcaatacc tttttggcat cgtcccagaa atgagatttt
     3301 tcttcgggga taaacttttc ggtatcttca ggttcgcggg tagcggctag ggcgaaccag
     3361 gataaggcga agaaaatgct ggcaacgaag aaacagaccg caaagtccca gggagaggcg
     3421 agggcgttaa gtaggtatcc cgctgcgatg gcagatccgc tgatgaagag atttgccagt
     3481 ccggcttgca tcccaaagaa agtgccacgt gactcgggtg gaatgatctt ggagaccatt
     3541 gaggtccacg cattggcggt aaaaccgccg ccaagccctt gccatatcag tagcaaaaac
     3601 gtaagcagca aaccggcatt cacgccaaca atggggagta agagtgctac cgccgcaaac
     3661 cctaaaaagg gaacacgttc atgaatggta tttagtaaca cagtgcgttt tagttgacgt
     3721 aagcgagaga cgtgactggc agtaaatagt tgtgggaaaa gccagcctgc cgaatggatg
     3781 gcaggcacca agccgattaa ggttgcagat tgggtcatgg tggcgacaaa gagcggcagg
     3841 atagttccga aggaggcaaa ccccaacgcc acaccgaata gacttccatc ccatagcccg
     3901 gcggtaacat tgtgtttaag gtctttgcga atagcttgtt caagtgggga gagcataaag
     3961 tgattttacc ttatggtgtt gttggtacat tgtggggtgg aggtgtatgg acttattttt
     4021 cctccttgac aaagtaaacc tacttggtct aaaataaaaa cgaacgttcg ttcatttatt
     4081 ttctggagac gtcatggcag aagaaacaca taccaaaggc gagcgcactc gtattcaaat
     4141 tgaagatgct gcaattgagt tgttcattga gcagggctac catgctacat cgatgcgtca
     4201 aattgccgat cgggcagatt tggcattagg cggtatttac aaccacttca agagtaaaga
     4261 cgagatattc gaagccataa ttgttgataa acatccatat aaaaggcttt taccgcttat
     4321 tttggaagca gaagggcata cgatggagga ttttctcggt catgcggcgc gggtggttat
     4381 caaggaactg acgtctcagc cgtattacat caagttgatg ctgattgaga tcgccgagtt
     4441 taacggcgtg catggtgctg tgttgatcaa ggagatcggt cccaagttgt tgccggtctt
     4501 tgaacgaata ataaagactc gcaagaatct gcgcctgact aacccagcca tgctgatgcg
     4561 aacttttatc ggtatggtgg tttcgtacat gatcacagaa atgctcatat cgaattccat
     4621 gctaaataaa atgatgccta aaaatcccat cgatgcctat gttgatattt acctgcatgg
     4681 cattgttaaa tctgaggctt gattccatgt tcaatacccg tatcgtttcg attgttcgta
     4741 aagagtttgt gcagattttc cgcgacccgc gttcactggc gctgatcatc gttatgccca
     4801 ttattcagct gtttttgctg gggtatgccg ccaccacgga tgtgaagaac gtgcctatcg
     4861 ctgtgcttga ccaaagccaa agcgctcaat cacgtgaatt gttagatgct tttcgcgccg
     4921 ccgattactt ccgcattgat tatgtggtgg gttctacgga agagtatcaa ttgctgattg
     4981 agtcgggcaa gatccgtgct gcgttggtca ttccgcctga ttatgatgtt caggttttag
     5041 atggcaaagc acaagtcctt tttgtgttag atggttccga tggcagtgta ggcgcaacgg
     5101 cactttccac tgcgcggttg attggtcagt cgtactcaac caaggtgcaa acgagccaat
     5161 tcgcactgcg tggttcgggt tcattaccaa cacaacctgt ggaagtacgc actcaagttt
     5221 ggtacaaccc cgattttcaa tcggcatatt tcatgatccc tggcgtgatc gggatgatcc
     5281 tttctttcat caccaccatt ctcacagcta ctaccatcgt ccgtgaacgc gagcgcggca
     5341 cgatcgagca gctcatcgtt acgccaatcc gctcgtggga gttgatcgtg gggaagttgt
     5401 tgccctatgt cattttggct ttcgtggaga cctttgaaat tcttatcgtt gggcatcttg
     5461 catttgatgt gcccgtgcgc ggaagtctct tactcatcac cctagcttcg ggtttgttcc
     5521 ttatgtctag tttgggcatt ggcttgtttg cttccaccat tgccaacacc cagcaagaag
     5581 ccatgctgtc ggtcatgatg ttcaacctgc cgatgatctt tctctctggc tttttcttcc
     5641 cagtgcaagc catgccgcag tttttgcagt tcgtatctct ggcgattcca ttgcgctatt
     5701 accttgtggt gatccgcgca ctcatgctta agggcgtagg ctttgctgct attcaaagcg
     5761 agatcatttc ccttaccatt tttggcatcg ttattatggg cattgctgcc ttgcgcttta
     5821 gaaaacgcct cgactaattt tcaacttgta cttttaactt tcaattctgg agtctctcat
     5881 gcaaaataaa ctaccccctc tgcccgtccg catcgttatc gctgtgattg tgctcggcac
     5941 gctggcatac ttcggctttc aatcgctcaa agatgatacc aatggcagtc tgactgcttc
     6001 tggtaccatc gaagcgacca ttgtgaacgt cgcccctgaa accgctggca aagtgacgga
     6061 tgtccgcgcc gatgaaggcg aatcagtgac gaagggacaa tcgcttctct ccctcgacgc
     6121 aagcctgtta actgcccaac aagccgttgc ctctgccgca gtagattctg ccacagccgc
     6181 ccttgccgct gcccaaacca aatatgatca aacgctcgaa gccgctcttg ccgcccaagc
     6241 tgcacaacgc tccaaagatt ggcaggcttc cgcgccaagt gagtttgacc aacccaattg
     6301 gtacatcgaa caagaccagc aggtttcatc ggctcaagca gaacttgatg ccgccaaagc
     6361 cgaaattgat gcagccaacg aaaacttgga ccgtgtcatt acagacctga agaattctga
     6421 gtttctaaaa acagaacaag aacttgccga tgctcgtgct gcatttttca ttgcacaaca
     6481 agtgaaagat caggcgtcca gcgcttcaga taatagcgga ttaactgatg ctgctcaaga
     6541 ttattatgac gatgctgtca cccgcctaaa tgatgcagaa gaagcatata acgacctgat
     6601 gagcagcgac gaagccgatg acgtgcgcta tgcccgcgga caagtcctcg tggcgcaaca
     6661 gcgttatgat gccgcctatg cccgcttgct caccttacag accggcgaaa attctcctgc
     6721 cgtgatcgcg gctggaaaag aattggataa ggcaaagtct gcactggcac aagcagaagc
     6781 cagcctggca ttggtccaag cacagattgc taaactcgaa gtccacgccc caatggacgg
     6841 agtcattctc acccgtaatg tcgagccggg agaatttgtc cagccgggtg cggcggctct
     6901 taccatcggc gacctctcag acctgaccat cacagtgtac gtcccagaga atcgctacgg
     6961 cgagatctcc cttgggcaga ccgccgaagt gactgtggac tctttccccg gcgtgacttt
     7021 cactgccacc gtcatccaaa ttgcgaacaa agccgagttc actccgcgca atgtgcaaac
     7081 tgtggaagga cgcagtgcca ccatgttcgc gatcaaactc agtgtgcaag atgccgaagg
     7141 acgtttgaaa cccggtatgc ctgcggatgt tatctttatt agtaaataat cggtggttga
     7201 gtcgctccgc gttttttatg gagcgtatcg aaaccaccag ttgaaggtag aaattatgga
     7261 tgattatcta gttaacgcaa ctggattaaa aaaatcgtac ggcgctaatc atgcagtaag
     7321 tggtgtttct ttgcatatcc ctcccggcga aatttatgga ctcgtcggtg cagatggggc
     7381 aggtaagacg accacaatgc gcttactggt tggggcgttg ctgccagatg ccggagaaat
     7441 ttcggtttgc ggatatgatg tgttaaagca aaccgaaaga gcacgttcaa ccattggcta
     7501 tctttcgcag cgcttctcga tgtatgaaga cctgaccgtg ttggagaata tccgtttctt
     7561 tgctgagatc cgcgggcttt cttccaccga atggctgccg cgttcgttgg agattctgga
     7621 ctttgtcggt ttggaaaaat tcaaagatcg ccgcgccggg caactttctg gcggcatgaa
     7681 gcaaaaatta ggtttagctt ctgcattggt cactcgtccg cgtttgctct tgctcgatga
     7741 acccaccacc ggcgtagacc ctgtgactcg tcaggatttt tggcaactgg tcattaagct
     7801 ggttggttcg cgtgaaggtg atggcgtttc ggtcatcatc tccacgccat acatggacga
     7861 agcctctcgt tgtcatcggg ttgggtttat gaagaatggc aggattatta cagaagatac
     7921 cccgtctaat ttgcgttcac ttctaaacgg acgtatcctt gaattgcgcg gctcaccttt
     7981 gaatactctg cggcatgttg cccataccga tgtggatgtg gaagatgtgc aagcctttgg
     8041 tgaccggctt cacatccgtg tgcgtgatga taaggcgaat caagtattgc gccgcttgaa
     8101 gtccagtatc aaagctgcgg gcgggcatgt ggttgagctc cgtatggtgc cccccgcctt
     8161 ggaagatgtg tttatcgctt tatctgaaga taaagccatc tctgctgtat cggtttaata
     8221 aaaggagcct gccatgatta acatcgattt aggtgtattg atcgaccgcc ctgtcaaaga
     8281 tgtttttgct tttgtttcca acccgaacaa catgacaaaa tggaattcag ctgtcatcaa
     8341 tttagagccg ctcacgccgg gcacagttgg tgtaggtgcg aagttcaaaa cgaccggcga
     8401 agccatgggg cggcgcatcg aaggtgaaat gcaaattaca gcctatgaac ccgataccaa
     8461 gtgcggcttt caagtcactg ccggacccat gcaagtgaac ttgaccatga tcttcaaaac
     8521 agtaggtaca ggtacaaaag ttagtttgaa tgcgcaagga aaccccggcg gtttattcaa
     8581 actggctgaa ggtgtcatgg cggggcaggt caaatctatg atggaaggca atctttctcg
     8641 cctcaaatct gcattggaag gatcgtaaat gtcactgcct gttatttatg ttgaaaatct
     8701 cacccggcgt tttggcgatt ttgtcgcggt ggatcatatc aactttgaag tgaacgaagg
     8761 tgaagt
//
LOCUS       DAOQFT010000020         8699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3823_length_8699_cov_6.970037, whole genome shotgun sequence.
ACCESSION   DAOQFT010000020 DAOQFT010000000
VERSION     DAOQFT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3823_length_8699_cov_6.970037"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLR93_00935"
     CDS             complement(<1..805)
                     /locus_tag="PLR93_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25549.1"
                     /translation="MMKRNQPILMFVAVLTIAALACNLPTTSNPADATEEPTEVSTEA
                     PEATPTEAPTEEASDSVSVSVSTATNCRTGPDVVYQLLMTVQPGSDFDVVGKYTPKGY
                     WIINMPTGGTCWLWGQYAIVQGDTSVLPEITPPPAPVAQPTSETSNQANNSNTNDDNE
                     EEDSQPSGSAPAAPTNIDITRQCTTLTQPGDLFPSFKMVVQFTWNDASDNETGFNIYK
                     DGSFYTTLPANTKNFTETFTVLLAQSPSITYGVQSVNANGSSGLVTGTVS"
     gene            983..1411
                     /locus_tag="PLR93_00940"
     CDS             983..1411
                     /locus_tag="PLR93_00940"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNH25550.1"
                     /translation="MQPSDIPNEKTAKRIGNQASFIILALVFSCVVSFFFLFNVDKIK
                     MLQTSIDLQFNGSTTTGTVISLEEFKSSAGHNAVFILTAEFEVDGQPYTVTGNAYYPT
                     LDHSWQGESLDVIYDPQDPNIAMINTFEERWVIPLAGALP"
     gene            1439..2248
                     /locus_tag="PLR93_00945"
     CDS             1439..2248
                     /locus_tag="PLR93_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25551.1"
                     /translation="MKSDVIFPLTRIIAGIVVLFLVAAFGILFFLPDQTGTLFAWAIK
                     PHMSSMFFGSAYLGGAWILAQTAFGPRWHRVQAVFPAVTIFTTAMLAATLPHWERFSL
                     GTIPFLAWLILYIVSPFLIPALWIYNRNTDSHEPEESDVTVPTTARLILRLFGGLVLL
                     LVTICYLIPSLIIPIWPWELTPLTARVLCGWLSVIGTLSLVNSFDPRWTSWRASFEGI
                     FIAYSLIFVAAFMNPAEFKTGIINWYTLFTAGMLIAIVIFYGIMESRRRKR"
     gene            complement(2245..5730)
                     /locus_tag="PLR93_00950"
     CDS             complement(2245..5730)
                     /locus_tag="PLR93_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH25552.1"
                     /translation="MIQAKPTYQELEQRCLTAESALAAIRSGQADTVLGEQGVLVLRL
                     KEAEKALQEAELKYRTLAENSPDIIYIIDLTKNKSGYLNRDTLLGYTLEELEKPGESL
                     LSKIHPDDLSAVKAHWQAALAGNMNAEMEYRLQLKDGSWEWLQSRPRILSWDVNGRPT
                     QLLVTLSIITERKQAEKRLRSMLDIAQTMSTSLDTKTTLQQIVDSITQVTGLDSGAIY
                     TLTGNQLYLATTAPALPPEMPDQFRFANLADHPHIQTTLAKGSSVIIPDTEVAELTEA
                     EREICITRGLRSIAYLPLMSARTAIGVLIVASTKKLRTFTDEELLLYHGFSVQAAQTI
                     ENAQLYDSARVHSNELEQQISERKQAEKKLQESEKLYRYAMEVAGTVPYYESYSEKHP
                     AVTYEFIGEGIRQITGYGPEEFNSQIWDSLVEKAIPVEELEGYSLDEAIQRTRSGEFP
                     IWKCEFRLHDRDGKTHWVFEAAVELRDANGNPVGSIGSYQDITIRKQAEEKLRKSEEQ
                     YRLLFEVASDGVVLHLLSTDRIKNRFRRFNQNICQMLGYTAEELAQLSPMDIQQPAEL
                     LKVPGEAEQLERNRELRFEKVFRRKDGSVFPSEVHSSVFELDGELLVLSIIRDISERK
                     QAEAKLRESEEKYRGLMEAIDSVVATIDYNGRFLYLNEVAAKWLGGTPQELVGKTMFD
                     LFPEPMALQQMEGIREVFQENQSKVSESLTYIQGQYRWFHNSLQPIHNELGEVVNVLL
                     HAVDVHELKVVQQELADLNRSLEQRIEERTIEVRQTNLALERALLAKDEFLANMSHEL
                     RTPLNGILGMAEILLEEEYGLLNERQHAYVNLIDSSGHHLLSLINDVLDLSKVEVGKL
                     ELDTEKVFIEDLCQSSMVFVRQLAKKKNVLVNYLPDPAISTLQADARRLKQILVNLLS
                     NAVKFTPEGGKVMLEVHANRDEGIVTFSVTDTGIGISEDNQKRLFQPFTQVDSSLSRK
                     YDGTGLGLALVKRLTELHGGTVTLQSEVGRGSCFTVSLPWREAGSTSHKLDAADAGDN
                     HKFAPSAQTIHATVLLVEDNTYNVMAISEYLEAKGYRLVFAVDGVDALAKAAESSPDL
                     ILMDIQMPNMDGLEATRRLRSDSAFASIPIIALTAHAMGGDRERCLAAGATDYLSKPV
                     KLKELESTIRKWLSS"
     gene            complement(5762..6058)
                     /locus_tag="PLR93_00955"
     CDS             complement(5762..6058)
                     /locus_tag="PLR93_00955"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNH25553.1"
                     /translation="MTANFKPNVLKKRYTLRLFVAGNATNSRIARENLEQLLARHPEH
                     EFEVEIVDLNVQPEFALDQGVFISPALQILEPSSGGIVYGNLSQKEVLEKVLNL"
     gene            complement(6048..7475)
                     /gene="kaiC"
                     /locus_tag="PLR93_00960"
     CDS             complement(6048..7475)
                     /gene="kaiC"
                     /locus_tag="PLR93_00960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HNH25554.1"
                     /translation="MATQTLKTPTKIKGLDEVLNGGVPTGGLTLLNGGPGTGKTLFGL
                     EFLVRGAQEGIPGILITFEEHEEALHHYATTFGWNTAMLEKQGLLSIIGARFDPEGLL
                     SGDFDLGGILAILRHKTEKLSAKQVVIDAPDVFLRLLDNRGKERAELYKLINWLRDEK
                     LTALMTVKADGGDVARSDYEFLDYLADCVIQLDQRVIEQVTTRRVRVVKYRGSAHGRN
                     EYPFAMTDQGIWIIPITETQLNHAALGVPFPTGVAGLDAYMSGGYLRASCNLVTGTSG
                     TGKTTFAASFARTSAANGKRVFYINFEESPDSMVSCMLSPGIDLRPAMEDGSLRFLSV
                     MPEAQGIEEHLIQAFRGIEELKPDIVIVDAISACRRMGSRNSAFDYLLRLIDHCKTIG
                     ITSLLTNLTDSVKETQEITGIDLSSMIDTVVILRNVEYNGQFQRELSVLKARGRQHSN
                     KVHKFQITDHGVSIVNSNIEDAHDR"
     gene            complement(7791..8498)
                     /locus_tag="PLR93_00965"
     CDS             complement(7791..8498)
                     /locus_tag="PLR93_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25555.1"
                     /translation="MLERLDLNLTLEREEYNKQLKALQAELHELAFQVYAQKRPVVIV
                     YEGMDASGKGGAIKRLTERIDPRGYVVWPIAAPSGEDKERHYLYRFWRRLPEQGQIAI
                     FDRSWYGRVLVERLEGFCTEAAWRRAYGEINQFERQLTDYGTIVFKFWMHISKQEQLR
                     RFKERTRTNYKAWKITDEDWRNRGKWEDYIVAAEEMLLKTSTPDAPWTLVEANDKYFA
                     RIKSLRTVAEKLRRALK"
     gene            complement(8491..>8699)
                     /locus_tag="PLR93_00970"
     CDS             complement(8491..>8699)
                     /locus_tag="PLR93_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25556.1"
                     /translation="YVEDMIARTDSPHAPWTIVESADRYYMRIKVFETIIASLETRLG
                     LAVSANEKTKRPRKTKEMKGAVNA"
BASE COUNT         2198 a         2155 c         1924 g         2422 t
ORIGIN      
        1 aactcactgt acctgtcaca agacctgaac tgccatttgc attcacagat tgcacgccat
       61 aggttataga aggagactga gccaataaga cggtgaatgt ctctgtgaaa tttttagtat
      121 tggcaggtag ggttgtatag aagcttccat ctttgtagat gttaaaacct gtttcattat
      181 cagaggcgtc gttccatgtg aactgcacta ccatcttgaa gcttgggaag aggtcaccgg
      241 gttgtgtaag cgtggtgcac tgacgggtaa tatcaatatt ggtaggtgca gcaggtgcag
      301 acccagaagg ttgcgaatct tcctcctcat tatcatcatt tgtatttgaa ttattcgctt
      361 gattcgaggt ttcacttgta ggctgagcaa caggagcagg cggcggtgtg atctcaggca
      421 gcacactggt atcaccttga acgatggcat attgacccca caaccagcag gttccgccag
      481 tcggcatgtt gataatccaa tatcctttag gagtatattt cccaacgaca tcaaaatcac
      541 tgcccggttg aacggtcatc aataactgat agacaacatc tggacccgta cggcaattgg
      601 tggcggtgct tacagagaca gacacgctat ctgaagcctc ttcagtgggt gcttctgtcg
      661 gtgtggcttc aggcgcttcg gtacttacct cggtcggctc ttcggtggca tcggctgggt
      721 tggaggtagt gggcaggtta caagctaagg cggcaatggt taaaacagcc acgaacataa
      781 gaataggttg atttctcttc atcattattg ctccatgaaa tgtattcgtc tcgtattata
      841 tgaagagtga aaaagacaaa catcccacag agggaggatg acttttggat ttatcacaaa
      901 tgcccccaaa ctcccattgc aaacattttg tttgttcgtg acggaacggt cttgttcgtg
      961 tttctttact aaggagaaca acatgcaacc atcagatatc cccaacgaga aaaccgccaa
     1021 acgcatcggc aaccaagcca gctttattat tcttgctttg gtcttttctt gtgtggtcag
     1081 ttttttcttt ctcttcaacg tcgacaagat caaaatgcta caaacctcca tagacctgca
     1141 attcaatggc agtacaacca ctggaacggt tatcagtctg gaagaattca aaagcagcgc
     1201 gggtcataac gccgttttca tcttaacagc agagttcgaa gtagatggac agccctacac
     1261 cgtcacaggc aatgcctact acccaaccct cgaccatagt tggcaaggcg aatcgttgga
     1321 tgtgatctat gacccacaag acccaaacat tgccatgatc aacacctttg aagagcgctg
     1381 ggtcattcca cttgcagggg cgttgcccta ggtaaacttt atcaatacag gaaccaagat
     1441 gaaatcagat gttatcttcc cccttactcg tattattgcc ggaatcgttg tattgtttct
     1501 agtcgccgcc tttggtatct tattcttttt gccagaccaa accggcacat tgttcgcatg
     1561 ggcaatcaaa cctcacatgt cctccatgtt ctttggttcc gcttatttgg gcggcgcgtg
     1621 gattctcgct caaacggctt tcggtccgcg ttggcatcgg gtgcaagccg tcttcccagc
     1681 ggtaaccatc ttcaccacag ctatgttggc tgccacgctt ccgcattggg agcgcttttc
     1741 tcttggcacc attcccttcc ttgcctggtt gatcttatat attgtctctc cgtttttgat
     1801 tcctgctctg tggatataca accgcaatac tgattcgcat gagccagaag aatcggatgt
     1861 gaccgtccct accacggcgc gacttattct ccgcctcttt ggtgggcttg tcctgcttct
     1921 cgttacgatc tgttatctca tcccaagcct gatcatcccg atctggccct gggagttgac
     1981 tccgctcacg gcacgggtct tatgcggttg gctttcggtg atcggaaccc tttcgttagt
     2041 gaactccttc gaccctcgct ggacgagttg gcgcgccagt ttcgaaggca tcttcattgc
     2101 ttacagcttg atcttcgtcg ccgccttcat gaatccggca gagtttaaga caggcatcat
     2161 taactggtac accctattta cagctgggat gttgattgcc atcgttattt tttatggcat
     2221 catggaatct cgcagacgca aacgctaact cgaaagccat ttacgaatag ttgattcaag
     2281 ttcctttagc tttacgggtt tactcaagta atcggtagca cccgctgcga ggcaacgttc
     2341 ccggtctcct cccatcgcat gagccgtaag ggcaatgata ggaatggaag caaaggcaga
     2401 gtcagatcgc aagcgacggg tggcttccaa accatccatg ttgggcattt gaatatccat
     2461 cagaatcaaa tccggagaac tctccgctgc tttcgctaga gcgtccacgc catctacggc
     2521 aaataccaat ctgtagcctt tggcttcgag atactcacta atcgccatta cattataggt
     2581 attgtcttcc actaataaaa cagtggcgtg tatcgtttga gcagaaggag caaatttatg
     2641 attgtctccg gcatccgcgg catctaattt atgtgaggta gaaccagcct cacgccaagg
     2701 caaagagacg gtgaaacaac ttccacgccc tacttcactt tgaagagtaa cggtaccgcc
     2761 atgcaactca gttaatcgtt tcaccagtgc cagccctaag cctgtgccat catattttcg
     2821 gctcaagctg gaatccactt gcgtaaacgg ctggaataga cgcttttggt tatcttccga
     2881 gatgccaatc cctgtatcgg tgacagagaa ggtgacaata ccttcatccc gattggcatg
     2941 tacctcaagc atgaccttcc caccctctgg cgtaaattta acggcgttgc tcaataaatt
     3001 aaccaaaatc tgtttgagcc gcctggcatc ggcttgtaac gtggaaattg ccgggtcggg
     3061 caggtaattc accagcacat tctttttctt tgccagttgt cttacgaata ccatactaga
     3121 ttgacaaagg tcttctataa aaactttttc agtatccagt tccagtttgc ctacttctac
     3181 tttggacaaa tcaagaacat cgttgatcag gctaagcaag tggtgtccac tggaatcgat
     3241 caagttcaca tatgcatgct gacgctcatt gagcaagcca tattcttctt caagcagaat
     3301 ttcagccatg cccaaaatgc cgttgagcgg agttcgaagt tcgtggctca tgttggctag
     3361 gaattcatct ttggcaagca aagcgcgttc taatgccaaa tttgtctggc gcacttcaat
     3421 cgttctttcc tctatacgct gttcaagaga tcgattcaaa tctgccaatt cttgctgtac
     3481 gaccttaagt tcatgaacat caacggcatg aagcaacaca ttaactactt cgcccaattc
     3541 attatggatc ggttggagtg agttgtgaaa ccaacgatac tgcccctgaa tataggtcag
     3601 tgattcactc actttactct gattctcttg aaagacctca cgtatgcctt ccatttgttg
     3661 caatgccatc ggctcaggaa aaagatcgaa catggtctta ccgaccagtt cttggggtgt
     3721 gccacccagc catttggcag caacctcatt caagtacagg aatctcccgt tgtaatcaat
     3781 cgtagccacc acactatcga tcgcttccat caagccgcga tatttttctt cactctcacg
     3841 taacttcgcc tccgcttgtt tgcgctcaga aatatcacgg atgatggaaa gcaccagaag
     3901 ctcgccatct aattcaaaga cactggaatg gacttcggat ggaaatacgg aaccatcttt
     3961 acggcgaaat actttctcga accttaattc acgattccgc tccaactgtt ctgcttctcc
     4021 gggcaccttg agaagttctg ctggttgttg gatatccatt ggactcaatt gcgccaattc
     4081 ctcagcggta tatcccaaca tctggcaaat attttgattg aaacgccgga accgattctt
     4141 aatgcgatca gtacttagca aatgcagcac caccccatca ctagctactt caaacaataa
     4201 gcggtactgc tcttcactct ttcgtagttt ttcctctgct tgtttacgaa tggtgatatc
     4261 ttgataagaa ccgatggacc cgacagggtt tccattggca tctcttaatt ccactgccgc
     4321 ttcaaaaacc caatgggttt tcccatcgcg atcatgcagc cggaattcac acttccagat
     4381 cggaaattcg ccagagcggg ttctttgaat ggcttcatca agcgaatagc cttcaagttc
     4441 ctctaccggt attgcctttt ccactaacga atcccaaatt tgtgaattga attcttcagg
     4501 tccatagcca gtgatctgac gaatcccttc tccaataaat tcatatgtaa cggcgggatg
     4561 cttctcgcta tatgactcgt aataaggcac cgtgccagcc acttccatag cataacgata
     4621 aagcttctct gattcctgta gcttcttctc ggcttgtttg cgttcagaaa tctgctgttc
     4681 caattcattg gaatgaactc tagcagaatc atataactgt gcattttcaa tcgtttgggc
     4741 agcctgaact gaaaagccat gataaagaag taactcttca tcggtaaatg tgcgtaattt
     4801 ctttgttgaa gcaacaatca atacccctat tgctgtacga gcactcataa gtggcaagta
     4861 tgcaatcgaa cgcaaacctc tagtaatgca gatttcacgc tcagcttcgg ttagttcggc
     4921 aacttcggta tcaggtatga tgacagaaga gccttttgca agtgtagttt gaatatgggg
     4981 gtggtcagcc agattggcga aacgaaattg atctggcatt tcgggcggta aagcaggcgc
     5041 ggtagttgct aaatagagtt ggttgcctgt cagggtgtag atcgcaccgc tatctaaccc
     5101 tgtcacttgc gtgatgctat caacaatttg ttgcaaggtc gtttttgtat caagagaggt
     5161 ggacattgtt tgagcaatgt ctagcataga acgtaaacgt ttttccgcct gtttgcgttc
     5221 agtgattata ctaagtgtaa ctaataattg agtgggccta ccattcacat cccaactcaa
     5281 gatgcgcggg cggctctgca accattccca actaccatct ttcaactgca aacggtattc
     5341 catctctgca ttcatgttgc ctgcaagtgc cgcctgccaa tgcgctttca cggcagaaag
     5401 atcgtcggga tgtattttgg aaagtagaga ctctcccggc ttttccaatt cttccagggt
     5461 gtatcccaat agggtgtcac ggttcaaata ccccgacttg tttttcgtta gatcaataat
     5521 gtaaataata tctggagaat tttctgccag agtgcgatat ttaagttctg cttcttgtaa
     5581 ggctttttct gcctctttca ggcgcaagac caatactccc tgttcaccaa gtacagtatc
     5641 agcctgtcct gagcggatcg ccgctaatgc ggactcagct gttaggcagc gttgttccaa
     5701 ttcctgatat gtcggtttcg cctgaatcaa ggggcacgct ccgtttcttt gcaaaaccag
     5761 atcaaaggtt caagactttt tccaaaactt ctttttgact taagttgccg tacacaatcc
     5821 caccagaact tggttcaagg atttgtaagg cgggggatat aaacacaccc tgatccaagg
     5881 caaattcggg ttgtacgttg aggtcaacaa tttcaacttc aaattcatgc tcagggtgtc
     5941 ttgccaacaa ctgctctaag ttttcacgcg cgatccgtga attggtggca ttcccggcaa
     6001 caaacaagcg cagggtataa cgttttttaa gaacgttagg tttgaaatta gcggtcatgg
     6061 gcatcctcta tatttgaatt cactatagaa accccatgat cagtaatctg aaacttatgg
     6121 actttatttg aatgttgacg tcctcgggct ttaagcacag acagttctcg ttggaattgc
     6181 ccgttgtatt ctacattgcg caaaataact acagtgtcaa tcatggaaga caggtcaatg
     6241 ccagtgattt cctgagtttc ttttacacta tcggttaggt tggtcaacag actggtaata
     6301 ccaatagtct tacagtgatc gatcagacgc aacaaataat cgaaggcaga attacgtgac
     6361 cccatgcgtc gacaagccga gatggcatct acgatcacga tatcgggttt caattcctca
     6421 atcccgcgaa atgcttgaat aagatgctct tcgattccct gcgcctcagg catcacagat
     6481 aagaacctca gagagccatc ttccatagct gggcgcagat ctataccggg gctaagcatg
     6541 caagatacca ttgagtctgg tgattcttca aagttgatgt aaaagacgcg tttgccattg
     6601 gcagcggatg tacgcgcaaa cgaagcggca aaggttgtct tacctgtacc agatgtacct
     6661 gtgaccaaat tgcaggaagc acgcagatac ccgccgctca tatatgcatc caaaccagcc
     6721 acaccggtgg ggaacggcac acccaacgcc gcatgattta gttgtgtttc cgtgatcgga
     6781 atgatccaaa tgccttgatc ggtcatggca aacgggtact cattccgtcc atgtgcagag
     6841 ccgcgatact tgaccacacg tacccgacgg gtggtgactt gttcgatcac acgttgatca
     6901 agttggatca cacaatctgc aagataatca agaaactcat aatcagaacg ggcaacatcc
     6961 cccccatctg ctttgaccgt catcagagca gtcaactttt cgtcacgcaa ccagttgatt
     7021 agtttatata actccgcacg ctccttgcca cgattatcaa gcaaacggag aaatacatcg
     7081 ggagcatcaa taacaacctg cttagctgaa agcttttctg ttttgtggcg caagatcgcc
     7141 agaatgccac ccaagtcaaa atcgcctgag agtaacccct ccgggtcgaa ccttgcccca
     7201 atgatcgaaa gaagtccttg tttttcaagc atggcagtat tccagccaaa tgtagtggcg
     7261 taatgatgca aggcttcttc atgttcttca aacgtaatta ggatgccagg aatcccctct
     7321 tgtgcgcctc gcaccaggaa ctctagccca aacaaagtct tgccagtgcc gggtcctccg
     7381 ttcaacaagg tcagtccgcc agtgggcaca cctccattaa ggacttcatc gagacctttt
     7441 attttggtag gagttttaag ggtttgggtt gccataaaat cagcattcct ttcaagagaa
     7501 taggatctgg ttatgtctca aacaaggcta gtctgcttgc ctaacgtatg gaactatttc
     7561 attgcaaata tgacattaca ctttaatatg atcatctctc gataccgatt tcatcgtctc
     7621 cccgcaatcc taaccgcaca aacgttaaaa ggctaaattt attttataca catttttgca
     7681 tttttacaca tgcttttcat tatgatttga gggtgttttc ctcccatttc tcggaaaata
     7741 gtgcgtaaaa caggcatccg atagggtacg gctcatgagg gcatagcaat tcacttcaac
     7801 gcgcgccgta atttctcggc gaccgtgcgc agggacttaa tacgggcaaa gtatttgtcg
     7861 ttggcttcta ccaatgtcca tggagcatcg ggggtgctgg tcttcaagag catctcttcg
     7921 gcagcgacga tgtaatcttc ccatttgccg cgattgcgcc aatcttcatc ggtgatcttc
     7981 caggctttgt aattggtgcg ggtgcgttct ttgaaacggc gcagttgttc ttgtttgcta
     8041 atatgcatcc aaaacttaaa gacgatggtg ccgtaatcgg tgagttgacg ttcaaattgg
     8101 ttgatctcac catatgcgcg acgccaagcg gcttcggtgc agaaaccttc gaggcgttcg
     8161 accaagacgc gcccatacca agaccggtca aagatggcga tctgaccttg ctcgggcaaa
     8221 cgtcgccaga agcgatatag atagtgccgc tctttatctt ctccagatgg agcggcaatg
     8281 ggccagacca cgtatccgcg cggatcgatc cgttcggtga gacgtttgat cgcgccgcct
     8341 ttgccggaag catccatccc ttcatagaca atcaccacgg ggcgcttctg ggcatagact
     8401 tgaaatgcca gctcatgcaa ttcagcttgc aacgccttga gctgtttgtt gtattcctcg
     8461 cgttcgagtg tcaagttaag atcgagtcgc tcaagcattg acggctccct tcatttcctt
     8521 tgtcttgcgc ggacgttttg ttttttcatt tgcagaaact gccaaaccca aacgggtttc
     8581 gagcgaagcg atgatggttt cgaatacctt gatgcgcatg taatatcgat cggcagattc
     8641 gacaatcgtc cacggagcgt gaggcgagtc tgtgcgggcg atcatatctt caacatagc
//
LOCUS       DAOQFT010000021         8476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3969_length_8476_cov_6.637216, whole genome shotgun sequence.
ACCESSION   DAOQFT010000021 DAOQFT010000000
VERSION     DAOQFT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_8476_cov_6.637216"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLR93_00975"
     CDS             <1..561
                     /locus_tag="PLR93_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25557.1"
                     /translation="AAPPWFYGIFLILIFAGVLHVLPFGGLVDSPPPATSFGYAMSVL
                     KHIILPASSIIVSSFFISVYNWRTFFLIYSSEDYVEMARAKGLPSRDIERRYILRPTL
                     PTIITNFALLIIGMWTGFTITETVFLWPGLGRTLFQAIGLYDTPVIVGSTIIYAYLLG
                     ITVFILDFVYALVDPRVKVGGDNSQQ"
     gene            558..1901
                     /locus_tag="PLR93_00980"
     CDS             558..1901
                     /locus_tag="PLR93_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25558.1"
                     /translation="MNNFAKSFRELLRYPSAVIGLITIIFLLVVSAYALITIPYGEAT
                     RLWRGGEDVWYQNPKYAAPAWMNLFSRVKQPVSFAVNTADGSMTKTVEVGAQDTSTIT
                     VVYSFDYAYDEPPQEMLLYFDSTFKDKLPFASVAWITPSGERIRVGDFAVDREMTFRI
                     SQDQKLMKRLKTDDVLPALFMEPKSDSTDLVKGTYQLEIIATTFEPDSDINVEFVLHG
                     KLFGLAGTDHYRRDLMLPLLWGTPIALTFGLLAALGTSLLTMIIAAVGSWYGGWVDEL
                     IQRITEINLVLPFLAILIMVGTFYSRSIWVILGVTILLSIFTGAIKSYRAVFVQSKVS
                     PYIEAARAYGTTDRRIIFSYLIPRMIPLLIPALVSSVPSYVFLEASLAVLGLGDPVLP
                     TWGKIIENAFAEGALYRGLYYWVLEPAALLMLTGLGFAMLGFALDRIFNPRLRDV"
     gene            1902..2864
                     /locus_tag="PLR93_00985"
     CDS             1902..2864
                     /locus_tag="PLR93_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25559.1"
                     /translation="MANNDNLLRLEDLVLHFGTTRGSVQAVDKVNFSLNANQAVVVLG
                     ESGCGKSSLAKAILRLLPRNVVSYSGRVYLNGNDVMQLDDEEFRQNIRWASISMVPQA
                     AMNSLNPVIRVGDQVAEPAIIHLGVSKQEAVSLVKKMFQHVGVPEDFITRYPFELSGG
                     MRQRVAIAMALVTSPSLILLDEPTSALDVLTQASIFNVLKRLKGELGISFILITHDIA
                     TSSELADSVAVMYAGQIVETSDAHRFFAEPLHPYSRMLMASVPRLRANTEPEFITGQP
                     PSLLEPPKGCRFADRCPKRFDKCSEEPPVVNVDGIQVKCWLYTS"
     gene            2903..3892
                     /locus_tag="PLR93_00990"
     CDS             2903..3892
                     /locus_tag="PLR93_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25560.1"
                     /translation="MTDTQRDVLLSINDLHVWFELRRLGFGHAGYVKAVDGVTFDLHY
                     GETIAIVGESGCGKSSLMKTILGLNKPTKGSITFDGKQDVGTLEGEMLRQYRAKIGYV
                     QQDPFGALAPFMNVQRILEEPLIINGIKDPAERERRVRKVMEEVKLTPVDDFLHKFPH
                     MMSGGQQQRVVIARAIILEPKLVVADEPVSMLDASVRVEILKLLRGLQEKYKLAVIYI
                     THDLSTVRYFSERVFVMYAGNVVEKAPVDSLVHNPKHPYTTALMDATSDPDANNALTY
                     RELPSGEPPSLVNPPSGCRFHPRCPKRIENLCEVEEPSDLKSDDHLVACWLYK"
     gene            3889..4116
                     /locus_tag="PLR93_00995"
     CDS             3889..4116
                     /locus_tag="PLR93_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25561.1"
                     /translation="MNVEHPERLIVLGVAMMLFGCIAPFLMVVKVWESTFFLNFLSYG
                     MSVLGMAIGFTGMAVMRVKQKRSDDDEDKYR"
     gene            4220..5242
                     /locus_tag="PLR93_01000"
     CDS             4220..5242
                     /locus_tag="PLR93_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25562.1"
                     /translation="MSDKKPLLEVKNLKTYFYTEDGVVRAVDGVDFEIYPGEVLGIVG
                     ESGCGKSVTSLSIMRLIGVPGKIVEGEINFDGKNLVTASEEEMRKVRGNRISMIFQQP
                     QSALNPVFRAGDQISEVLNIHQDFGKEAGRARAVELLKQVGIPEPNRRAEAFPHELSG
                     GMSQRVMIAMALACVPDLLIADEPTTALDVTIQAQILDLMRDLRNELGSALMLITHDL
                     GVIAEMANRVVVMYAGEIVEESPVASLFDKPHHPYTQGLIGSIPVLGQRRDRLDVIPG
                     NVPNLVNLPQGCRFAPRCRARTEHNLSICTEKHPGLIQVADGHKVRCWLYEDADGHTA
                     PLKSGK"
     gene            5257..6246
                     /locus_tag="PLR93_01005"
     CDS             5257..6246
                     /locus_tag="PLR93_01005"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNH25563.1"
                     /translation="MNKINKQEQPELLVVDNLVKYFPVRTGLFQRVTAWVQAVDKVSF
                     AVKKGETLGMVGESGCGKTTVGRTLLRLIEPTSGNVTFEGKDVLKLNQRELKPLRRDM
                     QIIFQDPYASLNPRMPIGESVMEGLQIHNIGHPKERWEIAVNMLKKVGLEEYHARRYP
                     HEFSGGQRQRIGIARALALNPKFIVCDEPVSALDVSIQSQVLNILKDLQEEFGLTYLF
                     IAHNLSVVEHISDRVAVMYLGKMVELTDRDSLYREPLHPYTKALLSAIPVGHPNEKRE
                     RTILKGDVPSPLNPPKGCRFHTRCPIAVSQCSQEEPTFKEVRPGHWVACLLAQ"
     gene            6335..8260
                     /locus_tag="PLR93_01010"
     CDS             6335..8260
                     /locus_tag="PLR93_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH25564.1"
                     /translation="MKKNVIPFLLVIVLIVIPACLSAPAQTETPAPTESFIEITETPP
                     PSLGPSLTVCLGQEPNSLYPFGDLNDAARNVLSAIYDGPIDNVGYEYQPVILTKLPSI
                     ENGDAQIVPVKINNGDSVVGADGNIIQFSKGSIVRPAGCRNADCAITYDGTSSIEMDQ
                     MIVTFRLRPDLTWSDGTPLTADDSIYSFELQKESKVNTYLTERTQTYEAADEQTLQWF
                     SIPGYLDPTYFLNFWQPAPKHAWSEFTPDQLPTVDVSSREPLGWGPYTVKEWLPGEGL
                     TLEKNPYYYRSRDGYPKLDSIQFRFIPDPDLALSELIAGRCDLIDPTINLEDKVGLLQ
                     EMQTSGQAKFFATTGMSMEWLGLGITPASYDNGYDIQKDRQNFFLDTYTRQAIAYCLD
                     RQTIATSVLHGLTEVPTSYLPLGHPALDTNLSAINYDPEIGNSLLDQAGWLDEDENPS
                     TPRRAINAQKVAYNTPLILNYQTTSTTQRRQVAAILEQSLANCGIGLNVEFISPNDLY
                     APGPVGPLFGRQFDLAEYALGVEGSEPPCGWFSTSSIPSEANSWSGTNVTGFSNEEYD
                     AACQAGQSALQEDESYLSAYRQTQIILADQLPAIPLFPRLHIAAARPDLCGFDLDPSA
                     RSLWNAEAFEISETCQN"
BASE COUNT         2087 a         2006 c         1963 g         2420 t
ORIGIN      
        1 gccgctccac cttggtttta tggcatcttt ctgatcttga tctttgcagg tgtactccat
       61 gtcctcccat tcggtgggtt agtggactca cctccgcctg ccacctcttt tgggtatgca
      121 atgagtgtcc ttaagcacat cattctgccg gcttcatcga tcattgtcag ttccttcttc
      181 ataagtgttt ataactggcg tacattcttc ttaatctatt ccagtgaaga ctacgtagaa
      241 atggcacgag caaaaggtct tccttctcgt gacattgaac gccgttatat ccttcgccca
      301 actttgccta caatcattac aaactttgca ttgctcatca tcggcatgtg gacaggtttt
      361 accattacgg aaactgtctt cctgtggcca ggcttgggac gaactctctt ccaggcaatc
      421 ggcttgtatg atacgcctgt gattgttggt tcaacaatca tttatgctta tttgttaggc
      481 attacagtgt ttatcttaga ctttgtttat gccttggttg acccccgcgt gaaggtgggt
      541 ggagataata gccaacaatg aacaattttg ccaaatcctt ccgtgaactg cttcgttatc
      601 cctcagccgt tattggtttg ataacgatca tatttttgtt ggtcgtttca gcttatgcgc
      661 tgatcacaat tccatacggc gaagcgactc gtttatggcg tgggggagaa gatgtttggt
      721 atcagaaccc caaatatgca gcccctgcgt ggatgaactt atttagccgc gtgaaacagc
      781 ccgtctcgtt tgctgtgaac acggctgatg gtagtatgac caagaccgtt gaagttggtg
      841 ctcaagatac atccacaatt acggttgtct attcgtttga ctatgcatac gacgaaccgc
      901 ctcaagaaat gctcctttac tttgatagca cattcaaaga taagttgcca tttgcttccg
      961 ttgcatggat caccccttca ggtgagagaa ttcgtgttgg cgattttgct gttgaccgcg
     1021 aaatgacatt ccgtatttct caagaccaaa agctgatgaa gagattaaag acagatgatg
     1081 tcttgccggc tctgtttatg gagccgaaga gtgatagcac cgatttagtg aagggtactt
     1141 atcaacttga gatcatagca actaccttcg aaccagattc tgatataaac gtcgaatttg
     1201 ttttacatgg taagttattt ggtcttgctg gcaccgacca ttatcgtcgt gacctaatgt
     1261 tgcctttgct ttggggaacc ccaattgctc tgacctttgg gcttctcgct gcattgggca
     1321 cctctctttt gacaatgatc attgccgccg ttggttcttg gtatggcggt tgggtcgatg
     1381 agttgatcca acgtattact gagattaacc ttgttttgcc cttccttgct attctgatca
     1441 tggtaggtac gttctactct cgtagtattt gggtcattct cggtgtgact atcttgctta
     1501 gtatcttcac tggtgcaatc aaatcatacc gtgctgtgtt cgtacaatcc aaagtttcac
     1561 cttatatcga ggctgcacgt gcatacggca cgacagatag acgcatcatt ttcagctatc
     1621 tcatccctcg catgatcccc ttgctaattc ctgccttggt ttcctctgta ccttcttacg
     1681 tatttttgga agcatccttg gcggttcttg gtttgggtga ccctgttttg cccacatggg
     1741 gcaagattat tgaaaacgcc tttgcagaag gcgccctcta tcgtggtttg tattattggg
     1801 tgttagagcc tgctgctttg ttgatgttaa ccgggttggg ttttgctatg ctaggctttg
     1861 cacttgaccg tattttcaat ccaagattga gggatgtata aatggcgaac aacgataacc
     1921 ttcttcgact tgaggatttg gttttacatt ttggcacgac ccgcggctct gttcaagctg
     1981 tggataaagt aaatttttct ctgaatgcta atcaggcagt tgttgtttta ggagagtcgg
     2041 gctgcggaaa aagctcgctc gccaaggcta ttcttcgcct tcttcctcgt aatgttgttt
     2101 cttattctgg acgcgtttac ttgaatggaa acgatgtgat gcagttggac gacgaagagt
     2161 ttcgccaaaa catccgttgg gcatctattt ccatggtccc gcaagcggcg atgaactcct
     2221 tgaaccctgt tattcgtgtg ggtgatcagg tggcagaacc tgccatcatc catttagggg
     2281 ttagcaaaca agaagctgtt tcgctggtaa agaaaatgtt ccagcatgtg ggcgtgcccg
     2341 aagactttat cacccgctat ccctttgaat tgagcggtgg tatgcgtcaa cgtgttgcca
     2401 ttgcgatggc gcttgttact tctccaagct tgatcctatt ggatgaaccc acctccgcgt
     2461 tggatgtatt gacccaagcc agcatcttta atgtgctaaa acgcctcaaa ggcgaactgg
     2521 gtattagctt tattcttatt acgcacgata ttgctacatc gagtgaactt gccgatagtg
     2581 ttgcggtgat gtatgcaggg caaattgtag aaacgagcga tgctcatcgc tttttcgcag
     2641 aaccgctcca cccgtattct cgtatgctga tggcaagtgt tccgcgtttg cgtgccaata
     2701 cggaacctga gtttattacc ggacaacccc ctagcttgtt ggaaccgcca aaaggttgtc
     2761 gttttgcgga tcgttgccct aaacgttttg acaagtgttc tgaagagccg cctgttgtaa
     2821 atgtagatgg cattcaggtc aaatgttggt tatacacatc ctagtttgtg taagataaaa
     2881 tctaaaaaga ggtctacaag acatgacaga tacacaaaga gatgtgcttc tctcgataaa
     2941 tgacctgcac gtttggttcg aattacgtcg cttgggtttt ggacacgccg gttatgtgaa
     3001 agcagttgat ggtgttacct tcgacttgca ctatggcgaa acgattgcta ttgtgggcga
     3061 gtcaggttgt ggtaaatcca gcttgatgaa gaccattctt ggtttgaaca aaccaaccaa
     3121 aggctccatt acgtttgatg gaaagcaaga tgttggaact ttggaaggcg aaatgcttcg
     3181 ccaatatcgc gcgaagattg gctacgtcca acaggaccca tttggtgcct tggctccatt
     3241 catgaatgtc caacgcattc ttgaagaacc gctcatcatc aacgggatca aagatcctgc
     3301 agagcgtgag cgccgagttc gtaaggtgat ggaagaagta aaactcaccc cggtggatga
     3361 ctttttgcat aaattccctc acatgatgag tggtgggcaa caacagcgtg ttgtgattgc
     3421 ccgcgcgatc attcttgaac caaagctggt cgttgcagac gaacctgttt ccatgctcga
     3481 tgcttccgtc cgcgttgaaa ttctcaagct tttacgcggc ttgcaggaaa aatacaaact
     3541 ggcggtgatc tacatcaccc atgacctttc aactgtccga tacttctctg agcgtgtttt
     3601 cgtaatgtac gctggaaacg ttgtggagaa ggcgcctgtt gattcattgg ttcataatcc
     3661 gaaacatcca tataccactg cgctcatgga tgcaacatca gacccggatg cgaacaatgc
     3721 tttgacatac cgtgagcttc cttctggtga accgcctagc cttgtgaatc ccccttctgg
     3781 ttgtcgtttc catcctcgtt gccccaaacg cattgaaaac ctctgcgaag tggaagaacc
     3841 tagtgattta aagtcagatg atcaccttgt agcttgttgg ttgtataaat gaacgtcgaa
     3901 catcctgagc gcctcatagt tcttggagta gcgatgatgc tatttggatg catcgcgcca
     3961 ttcctcatgg ttgtaaaagt ttgggagtca acgttctttt tgaacttcct ttcttacggc
     4021 atgtcggtgc tcggaatggc aattggtttc acaggtatgg cggtcatgcg cgtcaaacaa
     4081 aaacgttctg acgacgatga agacaagtac cgctaaattc attttttgac cctgaagaag
     4141 cctgcaaaag ctgaacggca attgcaggct tcttttcttt gttataatca gccgaaccta
     4201 acttttggga gccccttcaa tgagcgataa aaaaccactg cttgaagtga agaatctaaa
     4261 aacatacttc tacactgaag acggtgtagt ccgtgctgtg gatggcgttg attttgagat
     4321 ctacccgggc gaagtgctcg gtattgtagg ggaatcgggg tgtggaaaaa gcgttacctc
     4381 tctttctatc atgcgtttga ttggtgttcc cggaaagatt gttgaaggcg aaatcaattt
     4441 cgatggcaaa aaccttgtca ccgcttcaga agaagaaatg cgcaaggttc gtggaaatcg
     4501 tatctcgatg atcttccaac aacctcaatc tgccttgaat ccggtttttc gcgcaggtga
     4561 ccagatctct gaagtcttaa acattcatca agattttggc aaagaagctg ggcgtgcacg
     4621 tgcagttgaa ctgttgaaac aagtgggtat tcctgagcca aatcggcgcg cggaagcctt
     4681 ccctcatgaa ctctctggcg gtatgtcgca gcgcgtcatg atcgcaatgg cactggcttg
     4741 tgtccctgat cttctgattg cagatgaacc caccacagct cttgatgtaa ccattcaagc
     4801 gcagattctt gacttgatgc gtgacttgcg caatgaactc ggttctgctt tgatgctcat
     4861 tacacatgac ctcggcgtga tcgctgaaat ggcaaatcgt gtcgttgtga tgtatgcagg
     4921 cgaaattgta gaagaatctc cggttgcttc tctttttgac aaaccgcatc acccttacac
     4981 ccaaggcttg atcggttcca tcccagtttt gggtcaacgc cgtgaccgtt tggatgtcat
     5041 ccccggaaac gttccgaacc tcgtcaacct cccgcaaggc tgccgctttg ctcctcgttg
     5101 ccgcgctcgt acagaacaca acctttctat ttgtaccgag aaacatcctg gcttgatcca
     5161 agtagcagat ggacataaag ttcgctgctg gttgtatgaa gatgcggatg gacacactgc
     5221 accactgaaa tctggtaaat aaaggacgcg gcttagatga ataagataaa caaacaagaa
     5281 cagccagaac tgttggtggt tgataacctc gtcaagtact ttcctgttcg tacaggttta
     5341 tttcagcgtg taaccgcttg ggttcaagcg gtggacaaag tcagctttgc cgtgaaaaaa
     5401 ggcgaaaccc tcggcatggt gggcgagtct ggttgtggca aaacaaccgt aggacgcacc
     5461 cttttgcgcc tgattgaacc gacctctggc aatgtgacat tcgaaggaaa agatgtctta
     5521 aaactaaatc agcgtgaact aaaaccttta cgccgtgata tgcaaatcat ctttcaagac
     5581 ccatatgcat ctcttaatcc acgtatgcct atcggcgagt cggtcatgga aggtttacaa
     5641 attcacaaca ttggtcaccc gaaagaacgc tgggagatcg ctgtcaatat gctcaaaaaa
     5701 gtgggcttgg aagaatacca tgcccgccgc tatccgcacg aattttcggg cggtcagcgt
     5761 caacgtatag gcatagcccg tgctttggca ttgaatccca agttcatcgt ttgtgatgag
     5821 ccggtttcag ctttggatgt ttccattcaa tctcaggtgc ttaacatcct aaaagacctt
     5881 caagaagagt tcggtctcac ataccttttc attgcccata accttagtgt ggtggaacat
     5941 atctcagacc gtgtagcggt gatgtatctt ggcaaaatgg tcgagttgac tgaccgtgac
     6001 tcgttgtatc gtgaaccgct tcatccttac accaaggcat tgctttctgc catccctgtg
     6061 gggcacccga atgagaaacg ggagcgtacc attctcaaag gcgatgtgcc cagcccgctg
     6121 aacccgccaa agggttgtcg cttccacaca cgctgcccaa ttgcagtcag tcagtgttcg
     6181 caggaagagc caacctttaa ggaagttcgc ccaggtcatt gggtggcttg tttgttagcc
     6241 caataaaaaa taaagagacc gcacacgcgg tctcttttta taatagggga gtggttataa
     6301 ttacgaaacg ccttaaacag atatggcagg agctatgaaa aaaaacgtca tcccattttt
     6361 acttgttatt gttcttattg tgattccagc ctgtttaagt gcgcctgcac agacagaaac
     6421 cccggcaccg actgaatcat ttatagaaat tactgaaaca cccccgcctt ctttgggacc
     6481 ctcacttacc gtctgtttgg ggcaagagcc gaatagtttg tatccattcg gagatttgaa
     6541 tgatgcggct cgtaatgtcc tctctgcgat ctatgacgga ccgattgata atgtgggcta
     6601 tgaatatcag ccagtgattc tcaccaaact tccttctatt gaaaatggcg atgcgcagat
     6661 tgttcctgta aagataaata atggggactc tgttgttggt gccgatggaa atattattca
     6721 gttcagtaaa ggctccattg tacgccctgc tggctgccgt aatgctgact gtgccatcac
     6781 atacgatggt acctcttcta tcgaaatgga ccaaatgata gtgacatttc gcctacgccc
     6841 cgacctgact tggtctgacg gcactccgct cacagccgat gattccatct attcgtttga
     6901 actgcaaaaa gaaagcaaag tcaatacata tctcaccgag cgcactcaaa cctacgaagc
     6961 cgccgatgag caaaccctgc aatggttcag tatcccgggg tatctcgacc cgacctactt
     7021 tttaaatttt tggcagcctg ctccgaaaca tgcttggagc gaattcacgc ccgaccaatt
     7081 gcccactgtt gatgtctctt cccgtgagcc attaggatgg ggtccttaca ccgtcaaaga
     7141 atggttgcca ggcgaaggac tcacgctcga aaagaaccct tactattacc ggtctcgcga
     7201 tgggtatcct aaattggata gcattcaatt ccgcttcatt cccgacccag atctggctct
     7261 gtccgaatta attgcaggtc gctgtgactt gatcgaccct accattaacc ttgaagacaa
     7321 agttggattg ctgcaagaaa tgcagacttc tggtcaggca aagttttttg caaccactgg
     7381 catgagcatg gaatggcttg gcttgggaat tacacctgct tcttatgata acggctatga
     7441 tattcagaaa gaccgccaaa actttttctt ggatacatat acccgccaag ccattgctta
     7501 ctgtttagac cgccaaacta tagccacgtc cgttctccat ggactgacgg aagttccaac
     7561 cagttattta ccattgggtc accccgcgtt ggatacaaat ctctctgcca ttaactatga
     7621 ccccgaaatt ggaaattctt tgttagatca agcaggttgg ttggatgaag atgaaaaccc
     7681 ttccacgcca cgccgggcga tcaatgccca aaaagtggct tataacaccc cgcttatttt
     7741 gaactatcaa accacctcca ctacccaacg caggcaagta gccgccattt tggagcaatc
     7801 tcttgcaaac tgtggaattg gattgaacgt cgaattcata tcgcccaacg acctatatgc
     7861 tcccggtccg gttggaccct tatttggtcg ccaatttgac ctagcagaat atgctctcgg
     7921 tgtagagggt agcgaaccac catgtggttg gttcagtacg tcatcaattc caagtgaggc
     7981 gaactcatgg tctggtacaa atgtaacagg ctttagtaat gaggaatacg atgccgcctg
     8041 tcaggcgggg caatccgctt tacaggaaga tgaatcttat ctatcagcat atcgccagac
     8101 tcagatcatt ttggcggatc agttgccagc catcccgctt ttccctcgct tgcacatagc
     8161 ggctgctcgc cccgaccttt gcggtttcga ccttgaccca tctgcccggt cgctttggaa
     8221 tgccgaggca tttgagatca gcgaaacctg tcaaaactga ttgggaagca attcctgaat
     8281 cttgctataa tttagacatc gttatcaagt ttggatgtta ttaatccaaa cgaatttatt
     8341 ccattgattt caaacaacag gctggtatga ttcgaaacgt tttgttagtg gacgaccaac
     8401 gcgatattct acgacttttg cgttccacct tggacacgct caagaataag gatattcaga
     8461 tctacgaagc ccaatc
//
LOCUS       DAOQFT010000022         8475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_3971_length_8475_cov_6.464608, whole genome shotgun sequence.
ACCESSION   DAOQFT010000022 DAOQFT010000000
VERSION     DAOQFT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3971_length_8475_cov_6.464608"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PLR93_01015"
     CDS             <1..1408
                     /locus_tag="PLR93_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH25565.1"
                     /translation="HIPGLRVPTDKGLVGKVIQAGRGVVLPAIKHDPTLVEEDRLNGV
                     EMRALAIAPIQLHGKVIGVLEAVNPISGAFDPDALVVMTGLGNLAGTTIDNSKLFEQL
                     QNAHKHYRELFEDSIDPILVTDWEGKVLEANRQAVELSGYTTEQLHTMSIDQLHEVNW
                     GKVGLSFETIKAGTSCTYESGMLRQDGGRLPVEVYARRVEFEDSDSIQWILRDITARK
                     DLDALRDDMTSMIFHDLRSPLGNIVSSLEMMSTLMPEDETLSSMLNIAKNSTGRIQRL
                     VNSLLDINRLEAGQRIVDQNSIDPVALVREAIRDVEPSVTARQHVLTNRALSVLPLIW
                     VDVDMIHRVLINLLENSIKFTPAGGRIDIGAQTNEDGLFVRFWIRDNGPGIPAAERER
                     IFEKFTRLRGKNKAGGLGVGLAFCRLAVHAHDGDIWIESEEGKGTTFWLTLPVAQRKS
                     AGQIKRQTGRLTFKPDKP"
     gene            1468..>1826
                     /locus_tag="PLR93_01020"
     CDS             1468..>1826
                     /locus_tag="PLR93_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25566.1"
                     /translation="MQEITKNIFIEDQFLGVTLGVIVTPRGLIQIDAPPSPEDARSWR
                     ASLMNLGGGIDRLLINMDAHPDRTLGARAMDCTVVAHEKTAYIFRTRPSTFKAQGEET
                     GADWESIPGLGSVRWAPP"
     assembly_gap    1827..1926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1927..2480
                     /locus_tag="PLR93_01025"
     CDS             <1927..2480
                     /locus_tag="PLR93_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25567.1"
                     /translation="GADWESIPGLGSVRWAPPEISFVDQMTLHWGSSPVMLEHRPGPS
                     GGSIWVNLPEEKVLFVGDMVMKNQPPFLAGSNLKAWLESLDLLLEPEYKGYTFISSRG
                     GVVNSSAIKAQYDFLKHVHDKLNKSTSKKPNPAAVEKMVTSLLTWFKAPAARQKQFAQ
                     RLRYGLLHYNARQSYAPSNTIED"
     gene            2480..3535
                     /locus_tag="PLR93_01030"
     CDS             2480..3535
                     /locus_tag="PLR93_01030"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNH25568.1"
                     /translation="MNTSLPLLELMRGPVVEATHLGSIAVVTADGTLLHSYGDPHTVA
                     FLRSSAKPFQVLPFVEAGGVEHYNYTQRELALSCASHETSQMHLDAVTALQKKVGIEE
                     HDLQCGPHLPGDARKLREVIQQNILPTANFNNCSGKHTSMLAFAKMRGLPLTNYLAID
                     HPIQQDILKTLSEMCNIAANKIQLGVDGCSAPNFALPLFHAALGMARMCDTRDLSESR
                     ATACKKITTAMTSHSEMVSNFGEFDTELMKIAAGKIVTKRGAEGFQIMGIMPGVIHEQ
                     GVGVAIKVVDGDKHSMDNNLEEHTRVRPPVALEILRQLGALNEFQIQALSAFGPEKVL
                     KNYAGLVTGKLYPTFKL"
     gene            3532..4320
                     /locus_tag="PLR93_01035"
     CDS             3532..4320
                     /locus_tag="PLR93_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25569.1"
                     /translation="MTQLSDTFIQRLIARFHRPGVIGISMAGSYTRGMQDEYSDIDLE
                     LYVDTLPEDHYSLQLFDEKLVSVKYLRIADELSSLKIPERAVWAVSGLRNMHILMDES
                     GELAKLKQAAVDFNWAPLQPLADAYASEQLSGCAEEAHKLMGGLKTANESKVLYASWG
                     MFKNLSFAALVQAGLMIQSENRAFAILEEHFGKTHPAWTRAFRLAFGMDVETNVPAYQ
                     TRGKASLALYEETAKLFKGIINDNHREVIENTLQLINNFNREQK"
     gene            4348..5013
                     /gene="nth"
                     /locus_tag="PLR93_01040"
     CDS             4348..5013
                     /gene="nth"
                     /locus_tag="PLR93_01040"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNH25570.1"
                     /translation="MTDLKQRALQIHETLLKVFGEPIWRNPLPAIDELVSTILSQNTN
                     DVNRDRAFEALRAKFPTWEAVRDAKEKDVVEAIKPAGLANQKGPRIQQVLKAITEERG
                     ELNLQFLADMPIEDARNWLTKFNGVGPKTAAIVLCFSLNRPAFPVDTHVYRVTGRIGL
                     RPEKMTVEQAHPHLESIFPPETYYAAHLNIIRLGREVCNARKPMCPQCPVKHLCDFKE
                     KTQ"
     gene            5067..5534
                     /locus_tag="PLR93_01045"
     CDS             5067..5534
                     /locus_tag="PLR93_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNH25571.1"
                     /translation="METPPVSLALEKLNIPHQIFRHETPVDSFEKAASDRGQRPSQIV
                     RTILFRVSEEEFTLVLVAGPGQISWKLLRKLLGKSRITMATEEEVISVTGYRPGTVGP
                     FGLLKPARIMVEAGLLKEEEVSIGSGMRDTAVILKTNDLMKALGNVEVVELFE"
     gene            5570..6409
                     /locus_tag="PLR93_01050"
     CDS             5570..6409
                     /locus_tag="PLR93_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25572.1"
                     /translation="MSYKQRTIDFLEIEWATYIERFKRLPELDGVQRVRNQGYERFRD
                     MLAHVLAWWEDVMPVIVAIAENREFEKKKYDFDAFNAEAVAKYKDWDEAEFLAHFEKT
                     RQKAVADLKSMNEAAFEDRRIQGRINGIFIHHAREHLVALSKFLTLDTLEHEWGTYIA
                     RFDASEKKEEFLKKQSAARFGDLLAHNFAWWQEGVNAVQGALKDSSFVYKGPADTDAY
                     NAGIVAKYAGTSEEELRTIFEQKRLEMIELVRGLPDSAFQNPTIEEWLAADIVEHYDE
                     HAL"
     gene            6418..6903
                     /gene="folK"
                     /locus_tag="PLR93_01055"
     CDS             6418..6903
                     /gene="folK"
                     /locus_tag="PLR93_01055"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNH25573.1"
                     /translation="MEHIVYLALGSNMGNRLANLKNAISNLTPQMDVKKKSLVYETPP
                     WGYTDQPAFLNQVVMVETYMEPENLLGHLKRLETVLGREPTFENGPRVIDVDILFYDD
                     LVLNSPPLVIPHPRLHHRGFVLVPLNDIAPELIHPILGKSISELLLDVSRLNINEYKG
                     K"
     gene            6906..7664
                     /locus_tag="PLR93_01060"
     CDS             6906..7664
                     /locus_tag="PLR93_01060"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNH25574.1"
                     /translation="MRIIRYQTPEGTASYGWILEEKVGEVQGNMFGEYRRREARTPFK
                     ELKLLAPCEPSKIVCVGRNYVEHAKELGNEVPKIPLLFFKPPSSIIATSEHIVLPTQS
                     KQVEHEGELVVVMGKRAKNVTAEQAKEYIFGYTIGNDITARDLQKTDGQWTRAKGFDT
                     FCSFGPWIDTDFDPSDAVVTCRVNGQMRQMASTRDMVFNVGTLVAYISSVMTLEPGDL
                     IFTGTPAGVGELKNGDVVDVEIEGLGKLSNPVKA"
     gene            complement(7692..8267)
                     /locus_tag="PLR93_01065"
     CDS             complement(7692..8267)
                     /locus_tag="PLR93_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25575.1"
                     /translation="MHKYLKFFLLENLDTVRRIRVGSFWGVTLLITPWTWLSPFVFFG
                     LHFLLNLLNPRLPLDERLLQSIYFVIAVELTTPLHALGHIISGKLVHSAMDELLITAT
                     RDVNIYHGDQSQISGKVHLVRSLGGPILNILVGLFCILIAPSIPAGFWSGVNTSMIST
                     NLFFGLGGLLPVPSVDGEVIWREIINWMKKK"
     gene            complement(8269..>8475)
                     /locus_tag="PLR93_01070"
     CDS             complement(8269..>8475)
                     /locus_tag="PLR93_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25576.1"
                     /translation="RQGNSRVRSRLSSPMLILILPSLLIQMGRYASEVNWASPTLWFG
                     LLLFALTGFCGLTLAIGNWRESLT"
BASE COUNT         2249 a         2107 c         2079 g         1940 t
ORIGIN      
        1 gcatattcca gggctgcgcg tgccaacaga taaaggtttg gtagggaaag tgattcaagc
       61 cgggcgcgga gtagtgctcc ccgccatcaa acatgaccca actttggtcg aagaagacag
      121 actgaacgga gtcgagatgc gtgctcttgc tatcgctccg attcaattgc atggaaaagt
      181 catcggcgtt ttagaagcgg tgaatcccat ctctggggca tttgacccgg atgcattggt
      241 ggtgatgacc ggcttgggga atcttgctgg aacaaccatc gataattcga aattattcga
      301 gcaacttcaa aatgcgcaca agcattatcg cgagttattc gaagacagca ttgacccgat
      361 cctcgttacg gattgggaag gcaaagtgtt ggaagcgaac cgccaggcag tggagttgag
      421 tggctatacc accgagcagc ttcatactat gagcattgac caactccacg aagtgaattg
      481 gggcaaggtg ggtctgagct ttgaaacgat caaagctggc acctcttgta cgtatgaatc
      541 tgggatgtta cgccaagatg gcggcagatt gcccgtggaa gtatatgcac gccgagtaga
      601 atttgaagat tctgactcca ttcagtggat cttgagagac atcaccgcgc gcaaagattt
      661 ggatgcactg cgcgatgata tgacctccat gatcttccat gacttgcgct caccgcttgg
      721 caatatcgtt tcaagcctcg aaatgatgag cacgctcatg ccagaagatg aaacgttaag
      781 ctccatgctt aacatcgcca agaattctac tgggcgcatt cagcgtctgg tcaattcatt
      841 gctagatatc aatcgtctcg aagcgggtca acgcattgtg gaccagaatt caatcgaccc
      901 ggttgcgttg gtccgtgaag ccattcgcga tgtggaacca tcggtgacag ctcgtcaaca
      961 tgtgcttacc aaccgcgcat tgagtgtctt gccgttgatc tgggtggacg tggatatgat
     1021 ccaccgcgtg ttgatcaact tgttggaaaa ctccatcaag ttcacgcccg ctggcgggcg
     1081 cattgacatt ggtgcacaga ccaatgaaga tggtttgttc gtcagatttt ggatacgaga
     1141 taacggtccc ggcattcccg ctgcggaacg tgaacgcatt ttcgaaaagt ttacacgcct
     1201 gcgtgggaag aacaaagcag gcggcttggg ggtagggctt gctttttgtc ggcttgcagt
     1261 ccacgctcac gatggtgata tctggatcga aagtgaagaa ggcaaaggaa ccactttctg
     1321 gctcaccctg cccgtggcgc aacgcaaatc tgctggtcag atcaaacgcc agaccggtag
     1381 acttaccttc aagccagata agccataaaa agatagaaag aacaacgaag gacaatagat
     1441 ccgaaaggaa tttttttcag aaccatcatg caggaaatca caaagaacat tttcatcgaa
     1501 gaccaatttt taggcgtcac cttgggcgtg atcgtcaccc cgcgcggatt aattcagatc
     1561 gacgcgcctc cctctcctga agatgcccgc tcttggcgcg cctctctcat gaatttaggc
     1621 gggggcatcg accgtttact gatcaacatg gatgcccatc cagaccgcac cctcggcgcc
     1681 cgcgccatgg attgcaccgt tgtagcccat gaaaagactg cgtacatttt ccgtactcgt
     1741 cccagcactt tcaaagcaca aggcgaagaa accggcgccg attgggaatc cattcccggc
     1801 ttgggcagtg tgcgttgggc acctccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnccgg cgccgattgg gaatccattc ccggcttggg cagtgtgcgt tgggcacctc
     1981 ccgagatttc ttttgttgac caaatgaccc tgcactgggg cagctcccct gtgatgttag
     2041 aacatcgccc cggtccttct ggcggctcga tctgggttaa cttgcctgaa gaaaaagttc
     2101 tctttgttgg cgatatggtc atgaaaaatc agccgccctt ccttgctggc tctaacctca
     2161 aagcctggct cgaaagctta gacctcttgc tagagccgga atataaaggg tatacattta
     2221 ttagcagccg cggtggcgtg gtcaatagca gtgccatcaa agcacaatat gacttcctca
     2281 agcatgtcca cgacaaattg aataagtcca ccagcaagaa gccaaaccct gccgctgtag
     2341 aaaaaatggt tacgtcgttg ttgacttggt tcaaagcccc agcggctcgt cagaaacaat
     2401 ttgcccaacg tttgcgttac ggcttattgc actacaacgc tcgtcagagc tatgcaccca
     2461 gcaataccat agaagattaa tgaatacatc tctccctctt cttgaactca tgcgcggacc
     2521 tgtggttgaa gccacccacc ttggctccat cgcagtggtc acagcagatg gaacgttgct
     2581 tcactcttat ggcgaccctc acacggtcgc ctttttgcgc tcctctgcca aaccattcca
     2641 agtgctgcct ttcgttgaag ctggcggcgt ggagcattac aactacaccc aacgtgagtt
     2701 ggctctctcc tgcgcttcac acgagacctc acaaatgcat ctcgatgctg tgaccgcttt
     2761 acagaaaaaa gttggcattg aagagcatga tttacagtgc ggtccacacc tgccgggtga
     2821 tgctcgaaaa ttacgcgaag tcattcaaca gaacatcctc cccactgcaa acttcaacaa
     2881 ttgttcgggc aagcacactt ccatgttagc attcgccaaa atgcgcggac ttcccctcac
     2941 aaactatctt gctatcgacc accccattca acaagatatt ttgaagacac tttcagaaat
     3001 gtgcaacatt gccgccaata aaattcaact cggtgttgat ggctgctctg cgccaaactt
     3061 tgcactgcca ttgttccatg ctgccctcgg tatggctcgc atgtgcgaca cgcgtgacct
     3121 ttccgaatct cgcgccactg cctgcaaaaa aataaccacc gccatgacct ctcactctga
     3181 aatggtcagc aactttggcg agttcgatac cgaactgatg aaaattgcag caggcaagat
     3241 cgtcaccaaa cgcggcgcag aaggcttcca gatcatgggc atcatgcccg gcgtcatcca
     3301 tgaacaaggc gtaggtgttg ccatcaaagt tgtggatggt gataaacact ccatggataa
     3361 caacctcgaa gaacacaccc gcgtgcgccc gccagtggcg ttggaaattc ttcgtcaact
     3421 cggtgcgctc aatgagttcc agattcaagc gctatctgca tttggtccag aaaaagtatt
     3481 gaagaactat gcagggcttg tcaccggcaa gctctacccc acctttaaat tatgacccaa
     3541 ttaagcgaca catttatcca acgcctcatc gcccggttcc atcgaccggg cgttattggt
     3601 atttccatgg ctggtagcta cacacgcggg atgcaagatg aatatagcga tatcgatttg
     3661 gaattgtatg tagatacact gcctgaagac cattactcgc ttcaactctt cgacgagaaa
     3721 ctggtcagcg ttaagtacct tcgcatcgca gacgaattat cctcgctgaa aatacccgaa
     3781 cgagccgtgt gggctgtttc agggctcaga aacatgcaca tcctcatgga tgaaagcggc
     3841 gaacttgcca aactcaaaca agccgccgtg gatttcaact gggcacctct gcaacctctc
     3901 gcagacgcct atgccagcga acaactttct ggctgtgccg aagaagcgca caagctcatg
     3961 ggtgggttga aaactgccaa tgaatcaaaa gttttgtatg cttcgtgggg catgttcaaa
     4021 aatttatcct ttgcggcgtt ggtgcaagcc ggcttgatga tccaaagcga gaaccgcgcc
     4081 tttgccatcc ttgaagaaca ttttggaaaa actcaccctg catggacgcg cgcctttcgc
     4141 ctcgcctttg gcatggatgt ggaaacaaat gtcccggcgt atcaaacgcg cggcaaagca
     4201 tccttggcac tctacgaaga aacagcaaaa ttattcaaag gcatcatcaa cgacaaccat
     4261 cgtgaagtca ttgagaatac gctacaattg attaataatt tcaaccgcga acagaaataa
     4321 aacagctgct ttcttttaat aaacctcatg acagacctca aacaacgtgc tctacaaatc
     4381 catgaaactc tgctaaaggt ctttggcgag cccatttggc gcaatcctct gccggctatt
     4441 gacgaactcg tctccaccat cctttcgcag aataccaatg atgtgaaccg cgaccgtgct
     4501 tttgaggctt tgcgtgccaa gttccccaca tgggaagccg tgcgcgatgc caaagaaaaa
     4561 gatgtggttg aagccatcaa acctgccggg ctggcgaatc aaaaaggtcc gcgcatacag
     4621 caggtgttga aagccattac cgaagaacgt ggtgaactca acctgcaatt tctcgcggac
     4681 atgcccattg aagatgcccg caactggctg accaaattca acggcgtggg tcccaaaact
     4741 gctgccatcg ttttatgctt ttctctcaat cgccccgcct tcccggtgga tacccatgtc
     4801 tatcgcgtga caggtcgcat tggactgcgc cccgaaaaga tgaccgtgga gcaagcacac
     4861 ccccatcttg aatccatctt cccgccagag acctactacg ccgctcacct gaacatcatc
     4921 cgtttgggac gggaggtctg caatgcgcgc aagccgatgt gcccgcaatg cccggtcaaa
     4981 catctatgtg atttcaaaga gaaaactcaa tagaactaca ctgcgctgtt atggcgcagt
     5041 ttttattttc agttagaatg catgccatgg aaacacctcc cgtcagcctt gctttagaaa
     5101 aactgaatat ccctcatcaa atcttcaggc atgaaacacc tgtggattcg ttcgagaaag
     5161 ccgccagtga ccgtggtcag cgcccttcgc aaattgtgcg aacgattctc ttccgtgtga
     5221 gtgaagagga atttacgctc gtcctcgtag caggaccagg tcaaatttcg tggaagttgc
     5281 tgcgaaaatt actcggcaaa tcacgcatta ccatggcaac cgaagaagag gtgatatccg
     5341 tcaccggcta tcgcccaggg acagtgggtc cgtttgggtt gttgaagccc gcccgcatca
     5401 tggtggaagc gggtcttttg aaggaagagg aagtatccat tgggtcgggc atgcgcgaca
     5461 cagccgtcat cctcaaaacg aatgacttaa tgaaagcgtt aggaaatgtg gaagtggtag
     5521 aactgtttga gtaaaatcta actagcatct tatgatctgg agagtatcaa tgagctataa
     5581 gcaacgcacg atcgactttt tagaaattga atgggcaacg tatattgaac gctttaaacg
     5641 tctgcctgaa ttggatggcg tacagcgtgt tcgcaaccaa ggctatgagc gcttccgcga
     5701 tatgttggcg cacgtcttgg cttggtggga agatgtgatg ccagtgatcg tggcaattgc
     5761 agagaaccgt gagtttgaaa agaagaaata tgatttcgat gccttcaatg ccgaagccgt
     5821 ggcaaagtat aaagattggg atgaagccga gtttttggct cactttgaga aaacgcgtca
     5881 gaaagccgta gcagacctaa agtctatgaa tgaggcggcg tttgaagatc gtcgcattca
     5941 ggggcgcatc aacggcatct tcatccacca tgcccgcgaa catctggtgg cattgagcaa
     6001 attcctcacg ctcgatacgc ttgaacatga atggggaact tatattgcga ggttcgatgc
     6061 ttcagagaag aaagaagaat ttttgaagaa acaaagcgca gctcgttttg gcgacctgtt
     6121 ggcacataac tttgcatggt ggcaagaagg cgtgaatgct gtgcaaggtg ctttgaaaga
     6181 ttcaagcttt gtgtataaag gtccggcaga tactgatgcc tacaatgcag gcattgtggc
     6241 aaagtacgca ggaacgagtg aagaagaatt gcgaaccatc tttgagcaaa aacgtttgga
     6301 aatgatcgaa ctcgtgcgcg gacttcctga tagcgccttc caaaacccga ccattgaaga
     6361 atggcttgcc gccgatattg tggaacacta cgacgaacac gctctctaag gaaccacatg
     6421 gaacatatcg tttatcttgc acttggaagt aacatgggga atcgactcgc gaatttgaaa
     6481 aacgcgatct caaacctcac gcctcaaatg gatgtgaaaa agaagtcgct agtatatgag
     6541 actccgcctt ggggctatac agaccaacct gcattcttga atcaggtggt gatggtagaa
     6601 acatacatgg agcctgaaaa tttacttgga catttaaagc gcctcgaaac cgtcctcggg
     6661 cgggaaccta ccttcgagaa tggtccacgc gtgatcgacg ttgacatctt attctatgac
     6721 gatctcgtgc tcaattctcc tccactggtc attcctcacc cacgtttgca tcaccgcgga
     6781 tttgtgctgg tgccactaaa cgatattgcc cctgagctga tccacccgat tttaggaaaa
     6841 tccatcagcg aattgttatt ggatgtgagc cgattgaaca tcaacgaata taaagggaag
     6901 tagacatgcg catcatccgt tatcaaaccc cggaagggac tgcctcatac ggttggatcc
     6961 ttgaagaaaa agttggcgaa gtgcaaggga atatgttcgg cgaatatcgt cgccgtgaag
     7021 cgcggactcc cttcaaggag ttgaaacttc tcgcgccatg tgaacccagc aagattgtgt
     7081 gcgtggggcg taactatgta gaacatgcca aggaattggg caatgaagtg ccgaagattc
     7141 cgctgctctt tttcaaacca ccttcttcga tcattgcaac cagtgaacac attgttcttc
     7201 caactcaatc caagcaagta gaacatgaag gcgagttggt ggtggtgatg ggcaaacgtg
     7261 ccaagaatgt cacagccgag caggcgaagg aatacatttt cggctatacc attggcaacg
     7321 atatcacagc tcgtgacctg caaaaaacag acggtcagtg gacgcgcgcc aaaggcttcg
     7381 ataccttttg ttccttcggt ccgtggatcg acaccgattt cgacccgtct gatgcagtcg
     7441 tcacctgccg cgtcaacggg cagatgcggc aaatggcatc cacccgagat atggtgttca
     7501 acgtgggtac cttggttgcc tatatttcct cagtcatgac cctcgaaccc ggtgacttga
     7561 tctttaccgg gacacccgct ggggttggcg agctaaaaaa tggtgatgtt gtagatgtag
     7621 aaatcgaagg attaggaaag ttaagtaatc cggtaaaagc ataaaaaaag ccccgcaaat
     7681 gcggggcttt tttatttctt cttcatccaa ttgataatct ctcgccaaat cacctcccca
     7741 tctaccgaag ggacaggcaa cagaccgccc aacccgaaga atagattggt ggagatcatg
     7801 ctggtgttca cgccgctcca aaacccagcg ggaatggatg gagcgatgag tatacaaaac
     7861 aaacccacca gaatattgag aatgggtccg cccaacgaac ggacgagatg aaccttaccg
     7921 gaaatttggc tttggtcgcc gtgatagatg ttgacatcac gcgtggcagt gatgagcaat
     7981 tcgtccatgg cgctgtggac gagtttgccg ctgatgatat gcccaagggc atgaagcgga
     8041 gtagtcaact cgacggcgat gacaaagtaa atggactgaa gtagcctttc gtcgagcggg
     8101 agccgtgggt tcaaaaggtt gagcaagaaa tgcaacccga aaaagacgaa aggactgagc
     8161 cacgtccacg gggtgatcag aagagtcaca ccccagaaag aaccaactcg aatccggcga
     8221 acggtatcaa gattttcgag caaaaagaat ttgaggtact tgtgcatgtt aggtcaggga
     8281 ctcgcgccaa ttgccgatgg caagagttaa accacaaaac cctgtcagcg cgaaaagcaa
     8341 taaaccgaac cacaacgttg ggcttgccca attgacttca ctggcatagc gccccatttg
     8401 aataagcaaa gacggcagga tgagaatcaa catgggcgag gaaaggcgtg agcggacgcg
     8461 gctatttcct tggcg
//
LOCUS       DAOQFT010000023         8386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4024_length_8386_cov_8.163846, whole genome shotgun sequence.
ACCESSION   DAOQFT010000023 DAOQFT010000000
VERSION     DAOQFT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4024_length_8386_cov_8.163846"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="glgP"
                     /locus_tag="PLR93_01075"
     CDS             <1..715
                     /gene="glgP"
                     /locus_tag="PLR93_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNH25577.1"
                     /translation="RGGFHPVQVVASGVLINPYALTIGFARRFATYKRASLVLSDVER
                     LLELINRPNMPVQIIFAGKAHPADDPGKKLIQQVYRTVKKAETGGRIVFIEDYEMNLA
                     RYLVQGVDVWMNTPRRPYEASGTSGMKAAINGVPNFSVMDGWWCEAYNGKNGWKIGDE
                     KKYASNEEQDAADAEDLYSTLEHQIIPKYYNRNPKDLMSPDWLAFMKDSMKTVIPQFS
                     TRRMVKEYVEKFYVPALKK"
     gene            787..1131
                     /locus_tag="PLR93_01080"
     CDS             787..1131
                     /locus_tag="PLR93_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH25578.1"
                     /translation="MFPEITVTQLSEKLKSDEKFILLDVRELPELSQAKIEDSRLEVT
                     PMSRLGSEGPNALSESARSGETPVYIMCHHGSRSAQVTMWLAQQGYKNIFNVRGGIDA
                     YARQVDSSVGFY"
     gene            1555..3150
                     /locus_tag="PLR93_01085"
     CDS             1555..3150
                     /locus_tag="PLR93_01085"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNH25579.1"
                     /translation="MKNNPNKLWIFVLALGWLFDFLFWKQAPGVNFAIFWTACLIVGF
                     YLLLSNGHRPHRTTLLLIPLFGFFAAVTFIRAEPMTTFLAYTFTMFTLTIFTVTYLGG
                     RWVQYALADYVGKLLSLVGSMLIRPITFTADVRKTQTEAGTQPSKYNFMPIVRGLVIA
                     IPIVMIFASLLASADVVFNQRLDDFVQIFKLENLPEYIFRMIYILVIGYALAGIFLHA
                     STESKDEKLVGEDKPVIPAFLGFIESSIVLGSVIALFAAFVVIQFQYFFGGTTNINVE
                     GYTYAEYARKGFGELVAVAFFALMMLLTLTGVTKRETETQRKTYSGLGIALVALLLVM
                     LVSAYQRLNLYEAAYGFSRLRTYTHVFLIWIGLLLITTIVLEILRKERMFTFAMLIAS
                     FGFAASLPILNVDAFIVNQNIQRELSGNDVEDLDSQYFIELSDDAVPPLVKALQTPSL
                     PDSVHEKVTAALACIRYQRDLQRNDREYSWQSFQFSRLNADNALKSAQAKIDVFELDE
                     SEYPTKAIAPSGEEYPCSQYYYD"
     gene            3269..3817
                     /locus_tag="PLR93_01090"
                     /pseudo
     CDS             3269..3817
                     /locus_tag="PLR93_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
     gene            complement(3848..4654)
                     /locus_tag="PLR93_01095"
     CDS             complement(3848..4654)
                     /locus_tag="PLR93_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNH25580.1"
                     /translation="MLELSNTRFAFLDVETTGLSPWFGDRICQIAVIVTEGKRIKRTL
                     DLLVDPERELSPSAIHVNGLDAVALKGKPRFAEIANEVEEALKDAVVVCHNAKFDIQF
                     LDNEFRKLGRTIEFPNLIDTLLLARDYYELPSYSLDQLSQDFHISANMQGSHALADAI
                     VMKNLFFTMADSLKTIDKTLDDLIGIYNSPAWPEEGIYLPTELSEAIHSGKKLFIQYM
                     DKDGEISERWVSPREVIGLADYLYLQAYCHSREADRTFRLDRILSVEAEK"
     gene            complement(4709..5545)
                     /locus_tag="PLR93_01100"
     CDS             complement(4709..5545)
                     /locus_tag="PLR93_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNH25581.1"
                     /translation="MTIIVADTTCGLPRALIAQRGIPLIPQVVMFGEESFHDDKDLDT
                     ALFLQKLKAAKALPKTAAPEPSLYYPILEEARSKNESVVVVAPTGKASGTVRSAQTAA
                     QEFPDVDVRVVDTQTISCNLGSLVLLADDMAKVGKSASQIVAKLEDMIPRGRIYFLVD
                     TLEYLAKGGRIGGAKRLVAELLEIKPILQIKDGQVESFEQQRTKKRALARLVEITKEQ
                     CSGGAESHLCVLQVEAEKEAQALVEELKSTIPVAEIPIYELPPAIVVHAGPRAMGIGF
                     FV"
     assembly_gap    5631..5730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5747..6592)
                     /locus_tag="PLR93_01105"
     CDS             complement(5747..6592)
                     /locus_tag="PLR93_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH25582.1"
                     /translation="MSSDILIVTNGTKESFPAVEQGAWLAKTLERKITLLGITEKLNP
                     AAIDDTHPLEEVFEKAVDVFNAQGAAYRLEVRNGSAEKIVPQEAKEQDSIVVLAPLGR
                     PQLQRLMAGRSIRNFIEAIEQPLLYVPSVRKTVQKILVSVGGLGYDVNAEHIAMQIAM
                     KSGAEIVFLHVVPPIDLNYPTAEAISKNWQYLATTDTPVGRSLRQSLENARKAGINAS
                     VKVRHGNVIEEILAESVEGKYDLLCMGSSYSVHSLRQMYSANVTSEIMERVQCPVMTA
                     RFRTE"
     gene            complement(6604..7140)
                     /locus_tag="PLR93_01110"
     CDS             complement(6604..7140)
                     /locus_tag="PLR93_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNH25583.1"
                     /translation="MNTSNPSKYSQLFWMFLKVNLLSPSGPASVGLLYKEAVGTLMTE
                     ERFVEAVGFSNFLPGSEALKLAMFVGYAAAGVPGVFTALLGAMLPPTVMMFIVAFILQ
                     QVQHEDWLTGFVKGMSPAVAALIVMVAWELFRVGQTKAIGKRTLIIAALSAIALWFDV
                     PSPFVLLGAGILGVILFR"
     gene            complement(7137..7658)
                     /locus_tag="PLR93_01115"
     CDS             complement(7137..7658)
                     /locus_tag="PLR93_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNH25584.1"
                     /translation="MDIHLQLFWTFLKVNLLSTSGPASVGLLYHEAVGRFVTEAQFVQ
                     AVGFSSVLPGSDALQLAMYIGYAAGGITGGLVALIASILPPTVIILGVTMILHRLRRE
                     AWVNSFVEGLTPAIAVLMVFTAWKIFEKGGGMSWFGWGIALTSFLAMWRKIPERFVII
                     IAGVIGVIFLSGS"
     gene            complement(7672..8196)
                     /locus_tag="PLR93_01120"
     CDS             complement(7672..8196)
                     /locus_tag="PLR93_01120"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNH25585.1"
                     /translation="MTLTPSNLYLGELNDTPLGDFRLVASDLGLIAVEWADNQPELDA
                     YLRKIKRSCEPNQRKIAPYLKELREYLNGKRTTFTIPIDWTPFTPFQREALQAVFRVP
                     YGETRTYIDIAREIDHPHAYRAVGAANAMNPMPIIVPCHRILGTDGKLHGYGGGDGLP
                     TKEWLLKFEGVTMA"
BASE COUNT         2159 a         2175 c         1849 g         2103 t
ORIGIN      
        1 tcgcggcggg tttcacccgg ttcaggttgt cgcttcgggt gtgcttatca acccttatgc
       61 gttgaccatt ggcttcgccc gccgttttgc gacttataaa cgtgccagcc tcgtcctttc
      121 ggatgtagaa cgcttgctgg aactgatcaa ccgcccgaac atgccggtgc agatcatctt
      181 cgcgggcaag gcacacccag ctgatgaccc cggcaagaag ctcattcaac aggtttatcg
      241 cactgtcaag aaagctgaga ctggtggtcg catcgtcttc attgaagatt atgaaatgaa
      301 cctcgcccgc tacctcgtcc aaggtgtgga cgtgtggatg aatactccgc gccgccctta
      361 cgaagcgagt ggaacctccg gcatgaaagc cgccatcaac ggcgtgccca acttctcggt
      421 catggacggt tggtggtgcg aagcatataa cggcaagaac ggttggaaga tcggcgatga
      481 aaagaaatat gcctcgaacg aagaacagga tgctgctgat gcagaagacc tgtactccac
      541 acttgaacat cagatcattc cgaaatacta caaccgcaac cccaaagatc tgatgtcacc
      601 agattggctc gccttcatga aagattcgat gaagaccgtc atcccacaat tctccactcg
      661 ccgtatggtg aaggaatatg tagagaagtt ctacgtgccg gcgttgaaga aataatatca
      721 atgtagtggc ggggcttgtg ccacgaagtg gtattacccc gccactacat tattaaggaa
      781 ataaacatgt tccctgaaat taccgttacc caactcagtg aaaaactgaa atcggatgag
      841 aagttcattc tgttggatgt gcgcgaactc ccagagttgt ctcaagccaa gatcgaagac
      901 agcaggcttg aggtcacgcc aatgagccgc ctgggttcag aaggtccaaa tgctttatca
      961 gagtcggcac ggtcaggaga aacccccgtc tacatcatgt gccatcatgg cagccgcagt
     1021 gcacaagtca ccatgtggtt ggcgcagcag ggatataaaa atatcttcaa tgtgcgcgga
     1081 gggatcgatg cctacgcaag gcaggtggat agctcggtag ggttttacta aaaaaaggtc
     1141 acgcttttgc gtgacctttt tattcatctc tattaaacca gactgccacc cttgcgggtg
     1201 gcaatcgtgg ggcgctgtgg cttcctttga cttacttgag gagggatgcc acagcgctgg
     1261 gtttactact actatccatg ttagacatca cctcctcctt tcataaggat ggcgaattca
     1321 atttgtttgc ccccgtctgg tgagcaaaag tatgtcataa atcaaatcgg atgtcaatag
     1381 ggcgattttc gattctcaca tttctgtaat tttcgccttt gccgctccct gtaatttgcc
     1441 acaattttca aatttctgat accttttgag agtctccgca agccttgcag ggctatcctc
     1501 ggggaaactt tttaccgctt ccctatatcg tggtaaaaat aatttggagc ccttatgaaa
     1561 aataacccta ataaactctg gatctttgtc ctcgcccttg gctggctatt cgacttcctc
     1621 ttctggaagc aagctcctgg cgtcaacttc gccatctttt ggacggcgtg tctcatcgta
     1681 gggttctacc tcttactcag caatggacat cgcccgcacc gcacgacctt attgctgatt
     1741 ccactctttg gcttcttcgc cgctgtcacc ttcatccgcg ccgaaccaat gaccaccttc
     1801 cttgcttaca ccttcaccat gttcaccctg accattttca cggtcacata tctcggtggg
     1861 cgctgggttc aatatgcttt ggcagattat gtcggaaagt tactttcact tgtcggcagt
     1921 atgcttattc gccccatcac ctttactgcc gatgtacgta aaacccaaac cgaagcaggc
     1981 acacaacctt ctaaatacaa tttcatgccc atcgtacgcg gacttgtgat tgccattccg
     2041 atcgtgatga tcttcgcctc gcttctggca tctgccgatg tggtcttcaa ccagcgtctc
     2101 gatgattttg tgcaaatctt taaactagag aatctgcccg aatacatctt ccgcatgatc
     2161 tacattctgg tcatcggcta tgccttggca ggtattttcc tccatgcctc caccgaatca
     2221 aaagatgaaa agctagtcgg cgaagataaa cccgtcatcc ctgctttcct tggtttcatc
     2281 gagtcgtcaa tcgtacttgg cagtgtcatc gcactcttcg cagcgtttgt ggtcattcaa
     2341 ttccagtatt tctttggcgg cacgaccaac atcaatgtgg aaggctatac ctatgccgaa
     2401 tacgcccgca aaggttttgg cgaacttgta gctgtagcat tttttgcttt gatgatgctg
     2461 ctcaccctca ctggcgtcac aaaacgcgaa acagaaaccc agcgcaaaac ttattctggg
     2521 cttggcattg cactcgttgc acttttgctg gtgatgttgg tctctgcata tcaacgcctc
     2581 aacctctacg aagccgctta tggcttctca cgcttgcgca cttacaccca cgtcttccta
     2641 atctggatcg gcttactgct catcaccacc atcgtgcttg aaattttgcg caaagagcgt
     2701 atgttcactt ttgcaatgct gattgcatcg tttggctttg cagcctctct gcccatcttg
     2761 aatgtcgatg cctttatcgt caaccaaaat atccaacgcg aactaagtgg aaatgacgta
     2821 gaagatttgg actctcaata cttcattgaa ctctccgatg atgccgttcc acctctcgtc
     2881 aaagcgcttc agactccgtc cctgcctgac tccgtccatg agaaagtgac cgccgcccta
     2941 gcctgcatcc gctaccagcg cgatttgcag cgtaatgacc gcgagtattc atggcagtcc
     3001 ttccaatttt cgcgcctcaa tgcagataat gctctgaaat ctgcccaagc caaaattgac
     3061 gtattcgaac tcgacgaatc tgaatacccc acaaaggcaa tcgcaccaag cggcgaagaa
     3121 tatccctgct cacagtatta ctacgattaa tgctaggtaa gaatgctgtt tcattgaatg
     3181 tctgtcaatc atcaatcaca caaaaggaca tccaatggac ttcaaactaa ccatcttctc
     3241 cgattacata tgaccctggt gctatgtcgg ccagggtgtg gtcgagaggc ttaagaaaga
     3301 atataacgtg gaggtggaat ggcgtccgta ctatttacgc cccgatactc cgcccgaagg
     3361 catggaattg cctgattaca tcaaacgagc acgtgacaat ggctcggaag aacgcctgcg
     3421 aatgatggct gaaatgcacg gtatgccatt taggtctaca gaccgaattt acaacacacg
     3481 tctcgcccac gaagcgacag aatatgcccg cgaacatggc aaagccaatg agttccatca
     3541 catcatgttc cgcaaggtct atgctgatgg cttcgaccca gctcaatggg atgtgctccg
     3601 cgctacagca gaagaagctg gcttagaccc agacgatatg caaacggttg tagaaggtgg
     3661 caagtacacc aaagaggttg ctgggcaggt caattgggcg caacaaatcg gcgtaaccgg
     3721 tgtccccacc tatgttatca acgacaaata cgctattgtt ggtgcgcagc catatgaagc
     3781 gttcaagaac gcgttagcac aaatcttcaa agcataaaaa taatgcgacc cgaacgggtc
     3841 gcattattta tttctccgct tctacgctca aaattcgatc cagccgaaag gtgcgatctg
     3901 cttcacggga gtggcaatag gcttgtaggt acagataatc tgccagacca atcacttcgc
     3961 gtggcgaaac ccaacgttct gagatctcgc catctttatc catatattga atgaataatt
     4021 tctttccact atgtatagct tcgcttaatt cagttggaag gtaaatccct tcttcgggcc
     4081 aggcaggcga gttatagata ccaatcaggt catcgagggt cttatcgatc gtctttagcg
     4141 aatctgccat agtaaagaag agattcttca tcacaatcgc atctgctagg gcgtgtgatc
     4201 cctgcatgtt ggcagagata tggaaatcct gtgacagctg atcgaggcta taagaaggca
     4261 attcgtaata atcgcgagct aatagcaaag tgtcaatcaa gttgggaaat tcaatcgtcc
     4321 gcccgagttt gcgaaattca ttatccagaa attgaatatc gaacttggca ttgtggcaaa
     4381 caacaactgc atctttcagg gcttcttcta cttcatttgc aatttcagca aagcgcggtt
     4441 ttcctttgag ggcaacagca tccaagccat tgacgtgaat cgctgacggc gaaagctcac
     4501 gctcggggtc aacgagcagg tcaagggtcc gtttgatgcg tttgccctcg gtcacgatca
     4561 cagctatttg gcagatgcga tctccgaacc aaggtgaaag cccagtagtt tcgacatcca
     4621 gaaatgcaaa gcgggtattg gaaagttcaa gcatagtgcg ggcattataa aacaagaagt
     4681 cgtcagtact ttactgacga cttttctatt acacaaaaaa accaatcccc atcgcgcgcg
     4741 gaccggcatg cactacgata gctggtggta attcgtagat gggaatctct gcgacaggta
     4801 ttgtggactt caattcttca accaatgcct gcgcttcttt ctcagcctca acttgcagca
     4861 cacacaaatg gctttcagcg ccaccggaac attgttcttt ggtaatctct accaaacgag
     4921 ccaatgcacg ttttttggtt cgctgttgtt caaaagattc aacttgccca tctttgatct
     4981 gtagaatggg tttgatttca agcaactctg ctacgagacg tttggcgccg ccgatgcgcc
     5041 cgcccttggc aagatattct agtgtatcga ccagaaaata gattcttcca cgtggaatca
     5101 tatcttctag tttagcaacg atctgactcg ctgatttgcc caccttcgcc atatcatctg
     5161 ccaacagtac aagcgaaccg agattacagg aaatcgtctg tgtatccacg acacgcacat
     5221 cgacatctgg aaattcctgt gccgctgtct gtgccgagcg gacagttccg ctcgctttac
     5281 cagttggagc tacaacaacc acactttcat ttttgcttct ggcttcttcc aaaattggat
     5341 aatacagcga aggttcagga gcagctgttt tcggaagcgc tttcgccgct tttagctttt
     5401 gtagaaacag agccgtatcc aaatctttat catcatgaaa agactcttcc ccaaacatca
     5461 caacctgtgg aatcaatgga atcccacgtt gagcgatcag agcgcgggga agtccacaag
     5521 tggtatcggc aacaattatg gtcatggcag cctcctcatt tgattgtatt cccctttgaa
     5581 agctggttga actgtcaaaa gaactaagtt ttgtattaca aactttgcgg nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttgtatcgt tcatatttac tccgttctaa
     5761 accttgccgt catcacggga cattgaacgc gttccatgat ctctgaagtt acattggcag
     5821 aatacatctg ccgcaatgaa tgcacactat acgaagaccc catgcacaaa aggtcatact
     5881 tgccttccac agactcagca agaatctctt cgatcacatt tccatgtcgc actttcacag
     5941 aggcattaat gcccgctttg cgcgcatttt ccaacgattg gcgcaaactt cgtccaaccg
     6001 gtgtatctgt tgttgccaga tattgccaat ttttactgat cgcttcggcg gtcggataat
     6061 tcaaatcaat aggcggcacc acatgcaaaa agacaatctc tgcgccgctt ttcatcgcaa
     6121 tttgcatggc aatgtgttcg gcattcacat catagcccaa tccgcccaca ctcactaaaa
     6181 tcttctgaac ggtctttcgc acacttggca catacagcaa aggctgttca atcgcttcga
     6241 taaaattccg gatcgaacgc cccgccatca aacgttgaag ttgcggacgt cccaacggcg
     6301 ctaagaccac aatggagtct tgctccttcg cctcttgtgg cacgatcttc tccgcgcttc
     6361 cattgcggac ctcaagcctg tatgccgccc cctgagcatt gaatacatcc acggcttttt
     6421 caaaaacctc ttcgagcgga tgtgtatcat caatcgctgc cgggttcaac ttttcggtga
     6481 ttcccaacag cgtgatcttc ctttccaaag tttttgcaag ccaggcaccc tgttccacag
     6541 cagggaaact ttctttggtt ccatttgtaa cgatcaaaat atcggacgac ataacaattc
     6601 caatcaacgg aataagataa cccctagaat tcccgcaccc aataacacaa agggagatgg
     6661 cacatcaaac cacaatgcaa tcgcacttaa agcggcgatg atgagggtgc gtttgccaat
     6721 cgccttggtc tgccccacgc ggaacaattc ccacgccacc atcacgatca aggcagctac
     6781 ggcagggctc atccctttaa caaaccccgt cagccaatct tcatgctgca cttgttgcaa
     6841 gatgaatgcc acaatgaaca tcatcacggt cggtggaagc atggcaccca ataatgccgt
     6901 aaaaacaccc ggaaccccag cagcagcgta gcctacaaac atcgctagtt tcaaggcttc
     6961 gctccccggc aggaagttcg aaaaaccgac agcttcgaca aaacgttctt ctgtcattaa
     7021 cgtgccaacg gcttctttgt acaacaaccc caccgaagcg ggaccagaag gagaaagtaa
     7081 atttactttc aggaacatcc agaacaattg ggaatatttt gaagggttcg atgtgttcat
     7141 gagccagaaa gaaagataac gccgatgacc ccagcaatga tgatgacgaa gcgttcaggg
     7201 attttgcgcc acatggcgag gaaactggtc agtgcaattc cccaaccgaa ccaactcatc
     7261 ccgccgcctt tttcgaagat cttccatgcg gtgaagacca ttaaaaccgc aatggcaggt
     7321 gtaagacctt ccacaaagct attgacccat gcctcacgcc gaagacggtg caagatcatc
     7381 gtcacaccaa ggatgatgac agtcggcggc aaaatggaag cgatcaaagc gaccaagcca
     7441 ccagtgattc cgcctgccgc atacccaata tacattgcca gttgtaatgc gtcactacct
     7501 ggcagcacag atgaaaaccc tactgcctgt acaaactgcg cctctgtcac aaaacgccct
     7561 acggcttcgt gatacaacaa ccctaccgaa gcaggtcctg aggtgctcaa caaattaact
     7621 tttaagaaag tccaaaagag ttgaaggtgt atgtccacaa tctcgagtgt atcatgccat
     7681 cgtaacgcct tcaaatttta ataaccactc cttggtcggc agaccatctc caccaccata
     7741 gccgtggagt ttgccatctg tccccaaaat gcgatgacat gggacaatga tcggcatggg
     7801 attcatggca ttcgccgcac ctacagcccg atacgcatgc ggatggtcaa tttcgcgggc
     7861 gatgtcaata taggttcggg tttcgccata gggaacccgg aaaacagcct gtaacgcctc
     7921 gcgttgaaag ggtgtaaagg gcgtccagtc gatgggaatg gtgaaggtgg tgcgctttcc
     7981 gttaaggtac tcacgcagtt ctttgagata tggcgcaatc ttcctctggt tgggctcaca
     8041 ggagcgtttg atctttcgta ggtaggcatc cagctcgggc tggttatctg cccattcaac
     8101 ggcgatcaag cccaggtctg aggcaacgag gcggaagtct ccgaggggag tgtcatttag
     8161 ttcaccaaga taaaggttgg aaggagttaa agtcataggg tctgcctttg tcaaatacac
     8221 aataccaaaa gcaattacag acatgcaagt tgtcaaacaa tcgtagggca atcgtaggtt
     8281 ttgcgtcaag tagtattcgg ataaccatga taaatttacg tgcgtaaggt gatttctctt
     8341 ctcctccttt caaagagaac cgcggtcggc gtccgcggtt ctcgta
//
LOCUS       DAOQFT010000024         8360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4045_length_8360_cov_7.200000, whole genome shotgun sequence.
ACCESSION   DAOQFT010000024 DAOQFT010000000
VERSION     DAOQFT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4045_length_8360_cov_7.200000"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLR93_01125"
     CDS             <1..1044
                     /locus_tag="PLR93_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNH25586.1"
                     /translation="LSGAAGLESPTSTITTSVATEETAVVVEAPTATPLPAATGHIIF
                     AYNRDGQTDLYMTSPDGAELTRLTTGAAVDNSSPIRLSPDGTKIAFSATLGGNTDVYV
                     LDLTNNFTTRITDAQEKDSSPSWSPNSQQLAFESFRDGNIEIYIANADGSNPIRLTND
                     PAGDSNPIWSPVSNEILFTSNRFGNSDLMLLNLNGTVSTLTTSRAPDNAAAWSPDGNF
                     IAYQSFSGDISNICIIGRDGLNQVCPSIPAEYGTPVWSPNGTQIAASTLSNGVYSISL
                     FNSRDGSSQQISQPGIEPRGTPAWSPDGLRLVLQTQADGDMELFSVFVQTNEFTRITT
                     IAGYDGEPTWIVQ"
     gene            complement(1096..1854)
                     /locus_tag="PLR93_01130"
     CDS             complement(1096..1854)
                     /locus_tag="PLR93_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH25587.1"
                     /translation="MKNKKITLFVVLLIMGILLVACGGGTPPAEETQIVDATQAPVEA
                     TATIQPVSTVDLKLDPANLASDNAKAAVPYLYEGLVRLQDGAVVGALASTFTVSEDGL
                     DYIFDLRTGVTFHDGTLLNADTVVLNFNRWFDPADVNRGSGEYAAWVANFGGFKGELT
                     EDGKPKSPVDGIEKQDEFTVIIHLNTPDPDLLTKLTDPAFSIMSASSFAGNDGGTGPY
                     KAASNDGTTITLEPFAGYWDPAAIPSANVEIPAP"
     gene            1918..3474
                     /locus_tag="PLR93_01135"
     CDS             1918..3474
                     /locus_tag="PLR93_01135"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH25588.1"
                     /translation="MLPESPLAIIESRLRYLLPSEMYAEMWGNPSMEIMIRVHNHLRT
                     LQRIIHDYTSRQIDVSQLKPGQVKTEWQYGTMMFTDLAGFTKLMEANASKGRDGAENL
                     LKELTKYFSTMISIISKSGGELVEFTGDAMLVVFAKKKEKNDDAQRAIRAGLRMQRAM
                     KDFAEIQTPSGIVNLQMRIGIHSGRFLTAEIGTPRRMEHVLLGKDVQKAKLTESKGRN
                     ERVNVSVTAYELAKDSFRFDEGEPDYHLVVDDLTADSLGDYEITPMGRRMASSVLFDL
                     KDKKEVLSNITELLNSIEPLASYIPDPVLSMLVESAADRKIRPDFPTPTIMFVNFIGL
                     PEAADRALPGEERKLALSFSKAFSLINAAVEARGGVLKKVTYHLSGSDIVIYFGVPNA
                     HTNNEMRAASAAVAIREIVESINLKAPTIGGIKPEVYCQIGINMGPAFVAEVGDPRGR
                     REFNVLGDTVNTAARLMNRAQKNQIVLSETVKQAIESKYECTPLGEIELKGKSHPMSL
                     YELTKKKPKE"
     gene            complement(3518..3877)
                     /locus_tag="PLR93_01140"
     CDS             complement(3518..3877)
                     /locus_tag="PLR93_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25589.1"
                     /translation="MPGLFDRLDKEIKDNQKKDGISPLDIAKLPPAMRKIMRVMLREL
                     QASYPRMCEVVDAMPEQDRLTRTELDESLATLTEQLWLIRIGEKEKAIYKVNLRRKTG
                     STLMAGIWSSLDTKLKR"
     gene            complement(3889..4101)
                     /locus_tag="PLR93_01145"
     CDS             complement(3889..4101)
                     /locus_tag="PLR93_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25590.1"
                     /translation="MKCWHCEQEARASCSFCGRFVCKDHASTMSTFITMFVGANNTPK
                     GLAVANVIWCGECEPQPEPISMPELY"
     gene            complement(4111..4899)
                     /locus_tag="PLR93_01150"
     CDS             complement(4111..4899)
                     /locus_tag="PLR93_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNH25591.1"
                     /translation="MSMGKIVSIHSFRGGTGKSNTTANLAAQVALAGKRVGVVDTDIQ
                     SPGIHVLFGLDDEKMGKTLNDFLHGRATIREVGFSVGANTGTEDGRAKLAGKDIWLFP
                     SSIKGGEISQIIKEGIDFNVLNEGLQTAISEFDLDYLFVDTHPGLNEETLLSIATSDI
                     LIIILRPDNQDLQGTSVTVDVARSLDVPNLLLMVNKALPKHLNHPEFNKEIEGIFDAP
                     VTGVLPLSFDMAENASKDLFSLRYPDHEWSKALRNVVPFILNAK"
     gene            complement(4915..5652)
                     /locus_tag="PLR93_01155"
     CDS             complement(4915..5652)
                     /locus_tag="PLR93_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH25592.1"
                     /translation="MSGDILQKLSKTEFFKEFSEEALGAVAGKATLRHFAANEALMRK
                     GDPADSFFVILDGHVKIVTTGARGEEIIINKSNPGETIGELALVDERPRSAGVIALDD
                     VEALELTKDALFSLLDQRLDVSLGILRAFSNRLRFSTTYIEKVIDWSQKTAEGDYSFL
                     DATQPIGNSVRNDDDKAAQLLSTFYSMVHRVKAREEGLKQQVEKLTFQIDQERRKREF
                     EEITGTEFYAKLKEQAQTIRKKRSQDS"
     gene            complement(5649..6752)
                     /locus_tag="PLR93_01160"
     CDS             complement(5649..6752)
                     /locus_tag="PLR93_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNH25593.1"
                     /translation="MLVIALLIAFTFPIAFLILLDRFDLYNTGRFHFNLLTLAGGLVA
                     YFFAAQINPWVVNMGWASWGTVVRFVAPVVEEILKSLILIYLVQRADFNYVVDGALYG
                     FGAGIGFAVIENYEYVTGNAQIALVLALVRVFSTNLMHATGSGLIGTALAYSRGDKNK
                     RQGLLIILGGYAVAMLFHGLFNTMVNAGAFLVLAIAYGVIGLWLIWTIIRRGMTVQKD
                     WVAEKLGMADRVTLEETKVVKNIETVNEVLRPVEQRFGAEKASLVRNLIYKQAEIGIK
                     RKLLESVASEARKNEMEAIIKDLAAEVDVLRQNIGAYCMMMVREVYLGQDLQMWNLLN
                     ARVAAAGLGQKGGGLWDRMATRVKQNPDEAEKP"
     gene            complement(6882..>8360)
                     /locus_tag="PLR93_01165"
     CDS             complement(6882..>8360)
                     /locus_tag="PLR93_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNH25594.1"
                     /translation="GGNAGGSGNGGGNGGGGGGNAGGSGNGGGGGNGGGNAGGGSASD
                     PGTGDPSVQNPDGVVVRGSKQDSSDPCLDAGQLPCGKGDHGKGNPVNGSLHANCRAAQ
                     GEVDPVCAPKPPKDIIPDGGGNNGNGGGGGGGGGGTPPVVVDPPILPTPPVVEVTAAP
                     KVKVDDNGVASCPNWIMFHSFRSGNLDIFRLDGAEGVDGVQAINLSQDNSQDSRPSRS
                     PNDEFVVFQSDRDGNVELYLTDSKGSSQSRLTNTKSNNINAMFGPDSKSVIYQSDRNG
                     NWDIFLLNKDTGEELQLTSDAGDDVNPFYSPDNKWITFQSNRTGSWNVFVLDVSTGTE
                     YQVTSFSTDVLFPSWSPNGKQLAFLINMTGTWDLYVSDIQGKGFKQITTGGDAQNATW
                     SPEGDRLAYQVTYNGNTDIFTFDLTTNEEYQLTNFGGVDSAPTWNCGGTDVSFTSNRE
                     GDPNIYSTWWQGGGDIYGITVDPSTDKWSQWSPSKEPGSRGY"
BASE COUNT         2248 a         2215 c         1793 g         2104 t
ORIGIN      
        1 ctttctggtg cagcgggtct tgaaagccca acttctacta taactacatc tgtagccact
       61 gaagaaaccg ccgtcgttgt agaagcaccc acagccactc cgctccccgc cgccactggt
      121 cacattatct tcgcttacaa tcgagatggg caaacagatc tatacatgac ctctcctgat
      181 ggcgcagagc tgacccgtct caccaccggt gccgccgtgg ataacagcag ccctattcgc
      241 ctctcccccg atggaaccaa gattgccttt tctgccaccc ttggcggcaa taccgatgtc
      301 tacgtactcg accttaccaa taatttcaca acccgcatca cagacgctca agaaaaagac
      361 tcctcccctt cctggtctcc caacagtcaa caactagctt ttgaatcctt tcgtgatggc
      421 aacattgaaa tttatattgc caatgccgat ggttccaatc ccattcgctt gaccaacgac
      481 cccgccgggg acagcaatcc aatctggtca ccggtctcga atgaaatcct ttttacaagc
      541 aaccgcttcg gcaattcaga cttgatgttg ctcaacctca atggaacggt ctctactctg
      601 acaaccagcc gcgctcctga taacgccgcc gcttggtcgc cagacggaaa ctttatcgcc
      661 taccaatctt ttagcggcga tatctcgaat atctgtatca tcgggcgcga tggacttaac
      721 caagtttgcc catccattcc tgcggaatat ggaacaccag tctggtctcc taatggtact
      781 caaatcgccg ccagcactct ctcaaatggg gtttacagca tttccctttt caatagccga
      841 gatggtagct cacaacaaat aagccagccg ggaattgagc cgcgcggcac accagcctgg
      901 tcacctgatg ggcttcgatt agtcctacaa acccaagcag atggagacat ggaactcttc
      961 tctgtatttg ttcaaaccaa tgaatttaca aggattacca ctatagccgg gtatgatggc
     1021 gaaccaacct ggattgtaca ataaccaata ataaaagacc ccgaacgcaa tgttcggggt
     1081 cttttattat caaaattaag gagcagggat ttctacattc gcgcttggga ttgcggctgg
     1141 atcccaataa ccagcaaatg gttctaatgt aatggtagtg ccatcattag atgccgcttt
     1201 ataaggtcca gtcccaccat catttccggc aaaactcgaa gcactcatga tcgaaaaagc
     1261 tgggtcggtt aacttggtca ataagtcagg gtctggtgta ttcaagtgaa taatcactgt
     1321 aaattcgtcc tgcttctcga ttccgtcaac tggagatttc ggtttcccat cttctgtcaa
     1381 ctcgcctttg aagccaccaa agtttgccac ccaagctgca tattcacctg agcctcggtt
     1441 aacatcggcg gggtcaaacc agcgattgaa gttcaacact acagtatcgg cgttaagcaa
     1501 tgtcccatca tggaaagtaa cacccgttcg gagatcgaat atatagtcca atccatcttc
     1561 tgaaacagta aaggtggatg ccaatgctcc aaccacagca ccatcttgca aacgaaccag
     1621 accctcatac aggtagggca cagcggcttt ggcattatct gaagctaagt tagcaggatc
     1681 aagcttcagg tccacggtag agacgggttg aatagtagcg gttgcctcga caggtgcctg
     1741 tgtcgcatcc acaatttgcg tctcttctgc tggaggcgtt ccgccgccgc aggctacaag
     1801 taaaatcccc attattaaga gaactacaaa caaggttatt tttttgtttt tcatttttct
     1861 ctctgccttt agatagaatt gaaagtaaac atcttcaatt caagtataat ggaaatcatg
     1921 cttccagagt caccgcttgc cattattgaa agccgcttgc gttatctgct cccctctgaa
     1981 atgtacgccg aaatgtgggg caacccctcg atggagatca tgattcgggt tcacaatcat
     2041 ctgcgaaccc tgcaacgtat catccatgat tacacatcaa ggcagatcga tgtctcccag
     2101 ttaaaaccgg gacaggtgaa aaccgaatgg cagtacggaa ccatgatgtt caccgaccta
     2161 gcaggtttca ccaaactgat ggaagccaac gcatccaagg gacgtgacgg tgcggaaaac
     2221 cttctcaaag aattaacaaa atatttttca accatgatct cgatcatcag taaatcgggt
     2281 ggtgaactgg tagagttcac tggcgatgca atgctggtgg ttttcgcaaa gaaaaaggaa
     2341 aaaaatgatg atgcccagcg cgccatccgc gccgggttgc gtatgcagcg ggcaatgaaa
     2401 gatttcgcag agatccaaac cccttcggga atcgtcaatc tgcaaatgcg catcggcatc
     2461 catagcggca ggtttctaac ggcagaaatt ggtacaccgc gccgaatgga gcacgtgctg
     2521 ctcggtaaag atgtgcaaaa agccaaattg accgaaagca agggacgcaa cgagcgtgtg
     2581 aacgtgtctg tcaccgccta tgaactggca aaggacagtt tccgctttga tgaaggcgaa
     2641 cccgactatc acctggttgt ggacgacctg acagcggata gccttggcga ttacgaaatc
     2701 acacccatgg gacggcgcat ggcaagtagt gttctatttg acttaaaaga caaaaaggaa
     2761 gtcttgagca acatcactga actgctcaac tccattgaac ctctcgccag ttatattcct
     2821 gaccctgtcc tttcgatgct tgtcgaaagt gcggcagacc gcaagatccg cccagacttc
     2881 cccaccccta ccattatgtt cgttaacttt atcggtttgc cagaagcagc agaccgtgcc
     2941 ctgcctggcg aagagcgcaa attagcactc agtttctcga aagctttctc gcttatcaat
     3001 gcagcagtag aggcacgcgg cggggtgttg aaaaaagtga cctaccacct ttcaggttca
     3061 gatatcgtca tatactttgg cgttccaaac gctcacacca acaatgaaat gcgtgctgcc
     3121 tctgcagctg ttgcaatccg cgaaattgta gaatcaatta acctcaaagc gcctacgatc
     3181 gggggcatca agcctgaagt gtattgccag atcggcatca atatgggacc ggcattcgtg
     3241 gcagaagttg gtgacccacg cggcagacgt gagttcaatg tgctggggga tacggtcaat
     3301 acagcggctc gcttaatgaa ccgtgcccaa aagaatcaga tcgtcctctc agagacagtc
     3361 aaacaagcga ttgagtcaaa gtatgaatgc acgcccttgg gtgagatcga gctaaagggc
     3421 aaaagccacc ccatgagttt gtatgagcta accaagaaaa aaccaaaaga ataataaaaa
     3481 cgccgcaatc gcggcgtttt tatttaagta gaattggtta tcgcttaagt tttgtgtcga
     3541 gcgaagacca tatccctgcc attaaggtgc tgcccgtttt acggcgcaaa ttaactttat
     3601 agattgcctt ttccttttca ccgattcgaa tcagccatag ttgttctgtg agtgttgcaa
     3661 gagactcatc taattcggtg cgcgtgaggc ggtcttgctc aggcatggca tcgaccactt
     3721 cacacatccg cgggtagctt gcttgaagtt cgcgcaacat cacacgcatg atcttacgca
     3781 tggcaggcgg aagcttggca atatctaatg gagagatgcc atctttcttt tggttgtctt
     3841 ttatttcttt atcgaggcga tcaaaaagac caggcatggt tccctcgctt aatacaactc
     3901 aggcatactg atcggttcgg gttgtggctc acattcacca caccagatca cattagccac
     3961 tgccaacccc ttgggggtat tattcgcacc cacgaacatc gtaataaacg tggacatggt
     4021 cgaagcatgg tctttacaaa cgaaacgtcc acaaaatgag caggatgccc gggcttcttg
     4081 ttcacaatgc cagcatttca taagaggctc ctattttgca ttcaggatga acggcacaac
     4141 gttccgcaaa gccttcgacc attcatggtc tggatatcgc agagaaaata ggtctttgct
     4201 ggcattttct gccatatcga aagaaagggg caaaacgccc gttacaggcg catcaaaaat
     4261 cccttcgatt tctttattaa actcagggtg attcaagtgt ttcgggagtg ctttattgac
     4321 catcaacaaa agattcggca catccaaact gcgagcaaca tcaaccgtaa ccgatgtccc
     4381 ttgcaggtcc tgattatctg gtcgcaagat gatgatcaaa atatcggatg tagcaatgga
     4441 cagtaaggtt tcttcgttta acccagggtg tgtatctaca aaaaggtaat caaggtcaaa
     4501 ttcgctgatc gcggtttgca aaccttcgtt caatacattg aaatcgatac cttccttgat
     4561 gatctggctg atttcgccac ccttaatgct ggacggaaac aaccaaatat ctttcccagc
     4621 taatttggca cgtccatctt ctgtgccggt gtttgctccc actgaaaaac cgacctcccg
     4681 aatcgtggca cgtccatgca aaaagtcatt cagggtcttc cccatttttt catcatcaag
     4741 cccaaaaagc acatgaatcc ctggagactg aatgtctgta tccacaacgc caactcgttt
     4801 ccccgccaac gctacttgcg ctgctaaatt agcagtagta tttgatttac ccgtcccacc
     4861 acgaaaggaa tggatcgaaa cgatcttgcc catgctcatg aaaagcctcc gttgttatga
     4921 atcctgcgaa cgtttcttac gaatggtctg cgcctgttcc tttaatttag catagaactc
     4981 ggtgcctgtg atctcttcaa attcacgctt acgccgttcc tgatcgatct ggaaggtcaa
     5041 tttttctact tgttgcttca acccttcttc acgcgccttc actctatgca ccatggaata
     5101 aaaagttgaa agcaattgcg cagccttgtc atcatcattc ctgacgctat ttccaatcgg
     5161 ctgggtggca tctaaaaagg agtaatctcc ttctgctgtc ttctgcgacc aatcgattac
     5221 tttttcaata taggtcgttg aaaatcttaa tcggtttgaa aaagctcgca gaataccaag
     5281 agacacatcc aaacgttggt caagaagcga aaacaaagcg tctttggtga gttccaaggc
     5341 ttccacatca tccaacgcta tcacacccgc agaacggggg cgttcatcca ccaatgccaa
     5401 ttcgccaatc gtctcgccag ggttactctt gttgataata atttcttccc cacgcgcccc
     5461 cgtagtaacg attttgacat gtccatccag aatcacaaaa aaagaatcag cggggtcgcc
     5521 tttgcgcatc aaggcttcat tggcggcaaa atggcgcaag gttgcctttc cagcaacagc
     5581 ccctaaagct tcttctgaaa actctttgaa aaattcggtc ttactcagtt tttgcaggat
     5641 gtcgccactc atggcttttc tgcctcatct ggattttgct tcactcgggt cgccatccga
     5701 tcccataaac cgcctccctt ttgacccaag cctgcggctg caacacgggc atttaacaaa
     5761 ttccacattt gaaggtcttg tcccagatac acttcacgca ccatcatcat acaatatgca
     5821 ccaatatttt gtctcaacac atcaacttca gctgccaaat ctttaatgat cgcttccatt
     5881 tcgttcttac gcgcttcact cgcaacagac tcgagcaatt tacgtttgat gccgatctct
     5941 gcctgtttat aaataagatt acgtaccaaa ctagctttct ctgctccaaa acgctgttcc
     6001 accgggcgga gcacttcgtt taccgtctca atatttttga ccactttcgt ctcttcaaga
     6061 gtgacgcgat ctgccatacc cagtttttct gccacccaat ctttttgaac ggtcataccg
     6121 cggcgaatga tcgtccagat cagccataga ccaatgactc catacgcaat cgccaaaacc
     6181 aaaaacgctc cggcattgac catggtattg aacagtccat ggaagagcat cgcaaccgca
     6241 tagccgccca agataattaa tagaccttgg cgtttatttt tatcaccacg cgaatacgcc
     6301 aacgcagttc cgatcagacc gctacctgta gcatgcatga ggttcgtgga gaagacacgc
     6361 accaaagcaa gcactagagc aatctgagcg ttcccagtga catattcata gttctctatc
     6421 acagcaaagc cgatgcctgc cccaaaacca tagagtgccc catccactac atagttaaag
     6481 tccgctcgtt ggacgaggta gatcagaatt aaagatttta atatttcttc cactacggga
     6541 gccacaaatc ggaccactgt cccccaagat gcccatccca tattcacaac ccaagggttg
     6601 atctgtgccg caaagaaata cgccaccaag ccaccagcta aggtaagtaa attgaaatgg
     6661 aacctgccgg tattgtatag gtcaaaccgg tcaagaagga ttaaaaacgc aattgggaag
     6721 gtaaaagcga tcagtaaggc gatcacaagc atggcaaaat cataccttat ctagaaacga
     6781 cccgcaagga aaaatcatta acaagccgta tcggttttag ttgttaaaca aactccgccc
     6841 ttgagaagtt caagggcggg gaatttcaca aaatgttcag tctaatatcc acgcgaaccc
     6901 ggttctttgg atggcgacca ctgagaccac ttatccgtgg aggggtctac tgtaatgccg
     6961 tagatatcgc caccaccttg ccaccatgta gagtagatat tcgggtcacc ttcacgattc
     7021 gaagtaaagg acacatccgt gccgccacaa ttccaggttg gagcggagtc cactccgcca
     7081 aagtttgtta actggtattc ctcattggta gttagatcaa aggtaaagat atcggtattg
     7141 ccgttatagg ttacctgata tgccaggcga tcaccttcag gagaccaggt tgcgttctga
     7201 gcatcgccac cagtcgtgat ctgcttgaag cctttgccct gaatatctga aacatatagg
     7261 tcccatgtac cggtcatatt gatcaagaat gccaattgct tgccatttgg cgaccaactc
     7321 gggaagagca catctgtaga aaatgatgtt acttgatatt ctgtacctgt tgaaacatcc
     7381 agaacaaaca cgttccaaga accggttcgg ttagactgga aagtgatcca tttgttatct
     7441 ggtgagtaga aagggttaac atcatcgcca gcatctgaag tcaactgcag ttcttcaccc
     7501 gtatctttat taagaaggaa gatatcccag ttaccattgc ggtcactctg atagatcaca
     7561 cttttgctat cgggtccgaa catggcatta atgttattgg atttggtatt ggtaagccga
     7621 gactgtgagc tgcccttgct atcagtgagg tacagttcca cattcccatc gcggtcactt
     7681 tgaaacacaa cgaattcatc attgggagag cgactcgggc gtgagtcctg gctattatcc
     7741 tgacttagat ttatcgcttg aacaccatca acaccttcgg cgccatccaa acggaagata
     7801 tctaagttac cagagcggaa tgaatggaac ataatccaat tcgggcagga tgcaactcca
     7861 ttatcatcaa cttttacttt tggtgcagct gtcacttcaa ccacgggtgg agttggtaat
     7921 ataggagggt ccactacgac tggtggagtg ccaccgccac cgccgccacc accgccacca
     7981 ttcccattgt ttccaccacc atcagggata atatctttgg gtggtttcgg tgcacaaaca
     8041 gggtccactt cgccttgtgc ggcacggcaa ttggcatgga gacttccatt tacagggttg
     8101 ccttttccat gatcgccttt accgcagggt aattgtccag catctaaaca ggggtcagag
     8161 ctatcttgct tcgatccacg aaccacgacg ccgtcagggt tttggacaga cggatcccct
     8221 gttccagggt cactagctga accaccgcca gcattacctc cgccattgcc cccaccgccg
     8281 ccgtttccac taccaccggc attgccaccg cccccaccac catttccgcc gccgtttcca
     8341 ctaccaccgg cattgccgcc
//
LOCUS       DAOQFT010000025         8227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4140_length_8227_cov_5.061429, whole genome shotgun sequence.
ACCESSION   DAOQFT010000025 DAOQFT010000000
VERSION     DAOQFT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4140_length_8227_cov_5.061429"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLR93_01170"
     CDS             <1..291
                     /locus_tag="PLR93_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25595.1"
                     /translation="ALIPGGVLATLAGMTIAAERFGEFQTAGFFFLGLAFTFLLVALL
                     AGMKWAYWPALGLGVMGLLGLGSLLQFANYLWAVVLILVGGFLVVRNFIRNS"
     gene            complement(336..581)
                     /locus_tag="PLR93_01175"
     CDS             complement(336..581)
                     /locus_tag="PLR93_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNH25596.1"
                     /translation="MSVSTSAIRESQVRQLLENLSAYIEQFHGGTVEFVSLTGDDLKI
                     HLGGACLNCPLLPSTLHGWVEGTVHQFFPEINVVASN"
     gene            complement(584..1039)
                     /locus_tag="PLR93_01180"
     CDS             complement(584..1039)
                     /locus_tag="PLR93_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH25597.1"
                     /translation="MLDNLPGIPLFENLNPEQRFTLMSVFEKFACSPGTVIFEQGDIA
                     KYLYLILRGKAVISYKPYDGPRIVLSRLKDGDVFGWSAVVGGKKYSSSVASETSLETI
                     RIHRELLQNLLTQAPETGKIIIDRLALNVSPRWKNAHEQIKPLVHEERN"
     gene            1193..1396
                     /locus_tag="PLR93_01185"
     CDS             1193..1396
                     /locus_tag="PLR93_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25598.1"
                     /translation="MKKTKNLSSFEKLLLGLEKPEILEVDVPLRKGSACPNCNEGVLD
                     YNGMLQLECPSCGFVNGESGGCT"
     gene            1396..2022
                     /locus_tag="PLR93_01190"
     CDS             1396..2022
                     /locus_tag="PLR93_01190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH25599.1"
                     /translation="MTEINSREETNELDIMEQEIPANTTPLGRIMALVMIGFGFLALG
                     IAFLLLRNQDEVSTGSSIDFSTVPAKVDFPAPDLSFIDLGTDPVSLSDYRGSVVLVNL
                     WATWCPPCREEMPTLQAFYEEYKSEGFVLIAIDQGETLPQVLPFVKELKLTFPIWLDY
                     TSDAGRAFETMNLPSSYVIDRTGQVRLMWIGGISKKNLEKYVPDVIEE"
     gene            2030..2500
                     /locus_tag="PLR93_01195"
     CDS             2030..2500
                     /locus_tag="PLR93_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNH25600.1"
                     /translation="MDVTLNWQGKMAFEGAGPSGFVQRLDSDTSAGGDNSGARPMEMI
                     AIGLAGCTAMDVISILSKKQQKVADFQIKLHAERASDHPKVFTDSVIEYHVYGMDIDE
                     KSLVRAIELSAEKYCPAQAMLSKAFPIRLIYKIFDAETKSLLKEGEYQPKQKVA"
     gene            complement(2514..4004)
                     /locus_tag="PLR93_01200"
     CDS             complement(2514..4004)
                     /locus_tag="PLR93_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH25601.1"
                     /translation="MKKITKIKNSIQTITLVAVLFLSSCTSLPNNQTAASTSQPNNAV
                     EQSAVATAAFPTRRPYAPGELVDYVAQTGDTLPTLALRFNTSVDEILEANSFIPRDAT
                     TMPQGMPMKIPIYYRALWGSAFQIIPDHAFINGPTNIGFNTAAFLSTQTGWLSTYRAY
                     VGGEWKTGAALVDYAAINYSISPKLLLAILEYQGGALTQPAPPVNKNLLGLKRPFWDS
                     PYLQLVLAANTLNNGYYGWRTGTLLEFEDDDAILIRPDPWQNPGSVALQYYYSRMFTG
                     NDYNRAIGPDGLYKTYATFFGNPWENQFDLIPGSLSQPELSLPFPGDQTWSFTGGPHT
                     GWGTGFPFAALDFAPPAEQSGCVPAKPENFATAVADGLVVRSAQDGLALDLDRDGDER
                     TGWVIFYLHLATDQRATLGADLKTGDMIGYPSCEGGRSTGTHVHIARKYNGEWIPADS
                     ALPFVISGWVPHNGIEAYQGTLTKGGLTVTACVCGDAYTAISANYP"
     gene            complement(3988..5625)
                     /locus_tag="PLR93_01205"
     CDS             complement(3988..5625)
                     /locus_tag="PLR93_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH25602.1"
                     /translation="MQRTFAVFIALSMTISACGASPSIWGVVPTPTPNASQTAPLTDP
                     FAGQSDPIVFPTSTIPPFIETENAQTSTPQVTEEAVTTQVEPSITTAPLNDAAPFLYY
                     AQSGDMLSAVAARFGVSESEIISDADLTKTTLIDPGTLLVIPNRINEPTTPNVQLLPD
                     AEFVFSATSIGFDTEKFVKDQNGYLSSFRDYLGSVGWVQGYDAIDRLSVENSVSPRLL
                     LALLEYEARWVRGQPIDLLHTEFPMGFNDYHYKGMSVQMTWAINNMSIAYYGWRAGTI
                     THIEFPDGTRLRLDPRLNAGTVAIQYLFSKLHSESQWSQIINPDSGFPALYNEMFGDP
                     WARADLVGPIFPPGLIQPPLVLPFEPGAKWSFTGGPHNGWGQISPSIYGQSHSIYSAI
                     DFAPAAAKSGCVPNDAWVVAAAPGLVIRSENGVVMVDLDGDGYEQTGWDLLYLHLATK
                     DRVPAGTYLELNDRIGHASCEGGVATGTHLHFARKFNGEWVTADGPIPFVMSGWRVVA
                     GSAPYKGQLVKGDQVVTADTLSQAWSNIIREKDEEDN"
     gene            complement(5667..7274)
                     /locus_tag="PLR93_01210"
     CDS             complement(5667..7274)
                     /locus_tag="PLR93_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH25603.1"
                     /translation="MSFRKTVSVFLLAILIVSCAPATEEPATFPTYNPFVSTNGATLP
                     PNSNVAVTTPLSSETVIPSATSTRPPTPTRVPLNISSLIGVQSQIISGSPTPDTARVL
                     PTPRQDVDQYVVQAGDTLGAIAQRYNITLQTLMDANGITDPNALEVGMTLNIPAAQVT
                     VSSGQSLKIIPDSELVNGPATAKFSLRNFIASQNGYLNYYTQDVNGTLLNGADIIEAV
                     SYNYSVNPRILLALLEYQSGWVTNPTPTNIDYPMGYYDEARAGLYRQVVWAANTLNRG
                     YYLWKANGLSTWVLTDGQVLPIDPTINAGTAAVQYFFSQLYGYDAWQYATSQTGVVVT
                     YVVLFENPFNYAIEPLIPATLTQPQMSLPFAAGESWYFTGGPHGAWDSGSAWGALDFA
                     PPDNSGGCNISSSWVTAIADGLVTRTGTGSVYQDLDNDGYEQTGWVIIYMHVASAGRV
                     TPGEYLFRNEKVGHPSCEGGISNATHVHITRKYNGEWIAADGPYPWNLDGWISSGTGY
                     EYDGFLTRNGVSIEALDAAQPANLISR"
     gene            complement(7352..7798)
                     /locus_tag="PLR93_01215"
     CDS             complement(7352..7798)
                     /locus_tag="PLR93_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH25604.1"
                     /translation="MTTVRKLLEAKEFATNYSVETNQTVLDALKVMAEAHIGAVLVTD
                     GGKIVGIYTERDYLYKGEALGRVAKDTKIKDVMTPKMINVTMETTVEQCMGLMKQYKI
                     RHLPVVEGEHFVGLVSMRDVMLAAIDIKESEIRGLENYIMGSGFQS"
     gene            complement(7936..>8227)
                     /locus_tag="PLR93_01220"
     CDS             complement(7936..>8227)
                     /locus_tag="PLR93_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25605.1"
                     /translation="FVDAHTGNIPEEVRIIDVESDFQKSPFTHHLTAQSLMSICETVY
                     HRKSDAALLSVRGYQFLFSRLLSTPTAALVPEAVDLAWAWINAREPLKQAGD"
BASE COUNT         2147 a         1807 c         2077 g         2196 t
ORIGIN      
        1 gcgcttattc ccggtggtgt tttggcaacc ttggctggta tgacgattgc tgctgagcgt
       61 tttggcgaat tccaaactgc gggctttttc ttcctcgggc ttgcatttac cttcttgctt
      121 gttgctttgc tggcaggcat gaaatgggca tactggcctg cgttggggct aggtgttatg
      181 gggctactag gacttggctc gcttttgcag tttgctaatt acctttgggc agtcgtattg
      241 atccttgtgg gcggttttct tgtcgttcgc aacttcatcc gaaattccta gattctaaat
      301 gaatcaaaaa gccctcgcaa tttgcgaggg cttttttagt tgcttgctac tacattaatt
      361 tctgggaaga actgatggac tgttccttcc acccagccat gtaaggtgga tggaagcaag
      421 ggacaattca aacaagcccc tcctaaatga atcttcagat catctcccgt gagagagaca
      481 aactccacag tgccaccgtg aaattgctct atataggcgc tcaggttttc caaaagttgg
      541 cgcacctggc tttcacgaat agctgatgta gaaacagaca tcgttaattc ctttcttcgt
      601 gaacaagtgg cttgatctgt tcgtgggcat ttttccaacg aggagaaacg tttaatgcca
      661 atcgatctat gataatcttt cctgtttctg gagcttgcgt aagaaggttt tgcagcaatt
      721 cacgatggat acgaattgtt tctaggcttg tttcgctggc aacacttgaa gaatattttt
      781 tccccccaac cacagcagac cacccaaaca catctccatc ttttagacgc gaaagcacaa
      841 tgcgtggacc atcataaggc ttatagctaa tgacagcttt accccttaaa attaggtata
      901 aatatttggc aatatcgcct tgttcaaaga taaccgtccc tggagaacac gcaaatttct
      961 caaacaccga cattaaagta aacctttgtt cggggttgag attttcaaat aacgggattc
     1021 cgggcaggtt atctagcatt tgaaatcatg ttaaccattg tataaatacc ctaccagtat
     1081 catccatcct aaaccggatg acattcatcc tgttttctat ttgggtattt tcccttagtg
     1141 tatactttgt gaaaacggta atttttactt atattagacc ttcagaatct tgatgaaaaa
     1201 aacaaaaaac ctttcgtctt ttgaaaaact attacttggg ttagaaaagc cagaaattct
     1261 cgaagtggat gtgccgcttc gcaaaggctc ggcttgcccg aattgtaatg aaggggtatt
     1321 ggattacaac ggtatgcttc aattggaatg cccctcctgt ggctttgtta acggggagag
     1381 tggagggtgc acctgatgac cgaaataaac tcccgtgagg agactaacga attggatatt
     1441 atggaacaag aaatacctgc aaacacaacc cctcttgggc gtattatggc attggtgatg
     1501 atcgggtttg gattcctcgc actaggaatc gcttttttgc tgttgaggaa tcaggatgaa
     1561 gtatccaccg gctcttccat cgacttttca acggtgcctg caaaagtaga tttccctgcc
     1621 ccagacctga gttttattga cttgggcact gaccccgtgt ctctttcaga ttaccgtggc
     1681 agtgttgtgt tagtgaatct gtgggcaaca tggtgcccac cttgccgtga agagatgccg
     1741 actctacaag cattttatga agaatataaa tctgaaggtt ttgttttgat tgctatcgat
     1801 caaggcgaga cgttgcctca agtgcttcct tttgtgaaag aactcaagtt aacgttcccg
     1861 atatggttag actataccag tgatgccgga cgtgcattcg aaaccatgaa tttgcccagt
     1921 tcctatgtta ttgatcgtac tggtcaagtg cgcttgatgt ggattggggg tatttcaaaa
     1981 aagaatctag aaaaatatgt tcctgatgtg attgaggagt aaaaataaaa tggatgtgac
     2041 attaaattgg cagggcaaaa tggcatttga aggagcggga ccttctggct ttgtacaacg
     2101 cttagattca gatacttctg ctggcggcga taatagtggc gcacgcccca tggaaatgat
     2161 tgctataggt ctggctgggt gtacagctat ggatgtgatc tcaattcttt ctaagaagca
     2221 acaaaaggtg gcagattttc aaatcaaact tcatgcagag cgagccagtg accatcctaa
     2281 agtttttaca gactcggtga ttgagtacca tgtttatggt atggatattg atgaaaagtc
     2341 ccttgtccgt gctattgaac tttcggcaga aaaatattgc cctgcacaag ccatgctttc
     2401 caaagctttt ccgattcgat tgatatataa aatatttgat gcagagacaa aatctctgtt
     2461 gaaggaaggc gaatatcagc caaaacaaaa ggtcgcgtaa gcgacctttt gttttatgga
     2521 taatttgcac tgatggcggt ataagcatca ccgcatacac aggcagtgac agttaatccg
     2581 cctttggtga gggtgccctg ataggcttct atgccattgt gcggcaccca gccactaatt
     2641 acaaagggta atgcgctatc tgcaggaatc cattcgccgt tgtattttct ggcaatatga
     2701 acgtgtgtcc cagtagaacg cccgccttca caagacgggt aaccaatcat gtcgcctgtt
     2761 tttaagtctg cgccgagcgt ggcgcgttga tcggtggcaa gatgtaaata aaaaattacc
     2821 caacctgtac gttcatcacc gtcacggtcg aggtcgagag ccaagccatc ttgagcagac
     2881 cgcacgacca acccgtcggc tacagcggtg gcaaagtttt caggttttgc aggtacacag
     2941 cccgattgtt cggcaggagg cgcgaagtcc aacgccgcga atggaaagcc tgtgccccag
     3001 ccagtgtggg gtccacctgt gaagctccag gtttgatctc ctgggaatgg caaagaaagt
     3061 tctggctgcg ataaactgcc gggaatcaga tcgaattgat tttcccaagg gttgccaaag
     3121 aaagtggcat atgttttata aagcccatct ggaccaatcg ctcgattgta atcattgcct
     3181 gtgaacatgc gtgagtaata gtattgaagc gcaaccgaac ctgggttttg ccatgggtct
     3241 gggcggatga gaatggcatc gtcatcttca aattcaagga gagtccctgt gcgccagcca
     3301 taatatccgt tgttgagtgt gttagcggca agcacaagtt gtaaataagg tgaatcccag
     3361 aagggacgct tcagcccaag caggtttttg ttaacgggcg gggcaggttg tgtaagcgca
     3421 ccgccttgat attcaagtat tgccaataat aacttggggc taatgctata gttaattgcc
     3481 gcataatcta caagagcggc gcctgttttc cattctccac ctacataggc gcggtaggtg
     3541 gaaagccagc cggtttgagt tgaaagaaac gctgcggtgt tgaaaccaat attggttggt
     3601 ccgttgataa aggcatggtc tgggatgatc tgaaatgcac taccccaaag cgcgcgatag
     3661 tagatgggga tcttcattgg catgccttgt ggcatggtgg ttgcatcacg gggaataaat
     3721 gagttcgctt caagaatttc atctacgctg gtattgaagc gcaaagcaag ggttggtagg
     3781 gtatctccgg tttgagctac ataatctact aattcacccg gagcgtatgg acgacgagtt
     3841 gggaaagcag cagttgccac agcagattgc tccactgcat tattcggctg tgaagtggat
     3901 gcagccgttt gattgtttgg caaggatgta caagaggaaa ggaacaagac agctacaaga
     3961 gtaattgtct ggatggagtt ttttatttta gttatcttct tcatcttttt cgcggattat
     4021 atttgaccaa gcctgactca atgtatctgc tgtgacgact tgatcgcctt tgacaagttg
     4081 acctttataa ggggcgcttc ctgcaacgac ccgccaaccg ctcattacaa atggaatagg
     4141 accatccgct gtgacccatt cgccattaaa tttacgggca aagtgaaggt gtgttccggt
     4201 ggcaacaccg ccttcgcaag aagcatgacc gatccggtcg ttgagttcaa ggtaggtgcc
     4261 tgcagggacg cggtcttttg tagccaagtg gagatataaa aggtcccagc cggtttgttc
     4321 atagccgtcg ccatcaaggt ctaccatcac tacaccattt tctgagcgaa tgactaaccc
     4381 tggagccgct gcgacgaccc aggcatcatt tgggacacag ccagattttg ccgcagcggg
     4441 agcaaagtcg atggcggaat agatactatg agactgccca taaatacttg ggctaatctg
     4501 cccccagccg ttgtgtggac cacctgtgaa gctccattta gcgcccggct caaacggcag
     4561 gacaagcggt ggttgaatta agccgggagg gaaaatggga ccgactaaat ctgcgcgtgc
     4621 ccaagggtca ccgaacattt cgttatacaa ggctgggaag ccagagtccg gattgataat
     4681 ctgtgaccat tgggattcac tgtgaagctt tgaaaataaa tattgaatgg caacggtgcc
     4741 tgcattgaga cgagggtcaa ggcggagtct tgtaccgtcg gggaattcaa tatgcgttat
     4801 ggtgccagcg cgccaaccgt aataggcaat cgacatgttg ttgattgccc atgtcatctg
     4861 tacgctcatg cctttgtaat gatagtcgtt aaagcccatt gggaattcag tgtgtaacaa
     4921 gtctatgggc tgaccgcgca cccagcgcgc ttcgtattca agcaaagcca gcaacaagcg
     4981 cgggctgaca gagttctcga cggaaagacg atcaatggca tcgtaacctt gtacccaacc
     5041 gacagaccct aagtaatcac gaaacgaact gagatacccg ttttggtctt taacaaattt
     5101 ttctgtatca aaacctatag acgtggcaga gaaaacaaat tcagcatctg gcaaaagctg
     5161 aacattgggc gttgtgggtt cgttgatgcg gtttggaatg acaagcaggg tgccggggtc
     5221 gattagggtt gttttggtga gatctgcatc tgaaataatc tcagattcgc tcacaccaaa
     5281 gcgagctgca acggcagaga gcatatctcc gctttgggca tagtatagaa aaggtgcggc
     5341 atcattaagc ggagccgtgg taatggacgg ttccacttgg gttgtgacag cttcttctgt
     5401 aacttgcggt gttgacgttt gtgcattctc cgtctcaatg aaaggcggaa tggttgaggt
     5461 ggggaaaacg atcgggtcac tctgaccagc gaacgggtcc gtaagaggcg ctgtttgaga
     5521 ggcgtttggg gttggggtgg gaacaactcc ccaaatggaa ggcgaagccc cacaggcaga
     5581 aatggtcatc gatagtgcga tgaatacggc aaaggtacgt tgcatggttt gagtgattat
     5641 aaccagttag gttaatatca agtgagttag cgtgaaatga ggttggctgg ttgagccgca
     5701 tctaaggctt caatactaac gccgttacga gtgaggaagc catcatattc atagccggtg
     5761 ccgctggata tccaaccatc aaggttccaa gggtagggac catctgcggc aatccattcg
     5821 ccgttgtatt tgcgtgtgat atggacatgc gtagcgttcg agatgccgcc ttcacaagaa
     5881 gggtgtccca ctttttcatt gcggaaaaga tattcgccgg gtgtaacacg cccagcggat
     5941 gcgacatgca tgtaaataat gacccagcca gtttgttcgt agccatcatt atcaaggtct
     6001 tgatataccg agccggtacc cgtacgcgtg actagaccat ctgcaatagc ggtgacccaa
     6061 ctggagctga tattacaacc gccactgtta tctggcgggg caaaatcgag cgcgccccaa
     6121 gcagaaccgg agtcccaggc accgtgtgga ccacccgtga agtaccagct ttctccagcc
     6181 gcaaacggca gagacatttg cggctgtgtg agtgtggcag ggattaatgg ttcaatggca
     6241 tagttgaatg gattttcaaa aaggacaacg tatgtaacca ccacaccggt ttggcttgtg
     6301 gcgtattgcc aggcatcgta accataaagc tgtgagaaga agtattgcac agcggctgtt
     6361 ccggcattga ttgtagggtc aataggaagc acctgaccat ctgttaacac ccaagtagaa
     6421 agcccattag ctttccagag ataataaccg cggtttagtg tgtttgccgc ccaaacgacc
     6481 tgccgatata acccggcgcg ggcttcgtcg taatacccca tggggtaatc gatattggtt
     6541 ggtgttgggt tggtaaccca gccgctttga tattcaagca aggcaagtaa aatgcgcggg
     6601 ttgacggaat agttataaga cacggcttca ataatatctg cgccgtttaa taaagttcca
     6661 ttcacgtctt gggtgtagta attcaggtag ccattctggc tggcaatgaa attgcgtaaa
     6721 ctgaattttg ccgtagccgg tccgttgacc agttcggagt ctggaataat tttgagggat
     6781 tgtccgcttg atacagtaac ttgagcggct gggatgttta atgtcatgcc aacttctaat
     6841 gcatttgggt cggtgatgcc attggcatcc ataagagttt gaagggtgat gttataacgt
     6901 tgagcgatag cacctaaggt atcaccagct tggacgacat attgatccac atcttggcgc
     6961 ggagtgggca acacccgagc cgtatctgga gtaggtgagc cggagataat ctgactttgt
     7021 acaccaatga gagaagaaat gttcaaaggc acacgagtag gcgtgggcgg gcgcgtggag
     7081 gttgctgacg gaataaccgt ttcagaagac agaggcgtgg ttaccgccac gttgctattt
     7141 ggcggaaggg ttgctccatt tgtagaaacg aaagggttat aggtagggaa agttgcaggt
     7201 tcttctgtgg caggtgcgca agaaacgatg agaatagcaa gtaaaaacac agatacagtt
     7261 ttgcgaaaag acataaaagt gattttacca aatcgtatcg gtaaaaaaag aggcgcaaac
     7321 attgcgcctc ttttcaagtt tgttgagaga actaggactg aaaaccagac cccatgatgt
     7381 aattttcaag cccgcgaatt tcgctttctt tgatgtcaat cgcagccaac atcacatcac
     7441 gcatagaaac caaacctaca aaatgttcgc cttctactac aggtaagtgg cggatcttat
     7501 actgtttcat cagccccatg cattgctcaa cagtagtttc catggttacg ttgatcattt
     7561 tcggcgtcat cacatctttg atctttgtat ctttggcaac ccgccccaat gcttcccctt
     7621 tgtagagata atcgcgttca gtataaattc caacgatctt tccgccatct gttaccaaca
     7681 cagctccaat atgggcttca gccatcacct tgagggcatc caataccgtc tgattggttt
     7741 caacagaata gtttgtcgca aattccttag cttccagaag ttttcggaca gtggtcatga
     7801 ttttcgtctc cttatataag ttctttttta tcataggcaa aataaaacgt cacacggaaa
     7861 acctcctcat ctcgcgtgac gtttgtcatc tgatttattt ttgacccgtc tgcactcttt
     7921 gctttcggct catccttaat ctccagcctg tttcagcggt tctcgggcat ttatccacgc
     7981 ccaagctaag tccacagctt cgggcacaag agcagctgtc ggcgtggaaa ggaggcgcga
     8041 gaacagaaat tgatatccgc gcactgagag caatgctgca tccgatttac gatgatagac
     8101 cgtctcgcag atcgacatca acgactgagc ggtgaggtga tgcgtgaaag gtgatttctg
     8161 aaagtcactt tccacatcaa tgatgcgtac ttcctctgga atattgcccg tgtgcgcgtc
     8221 cacaaag
//
LOCUS       DAOQFT010000026         7888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4386_length_7888_cov_7.146176, whole genome shotgun sequence.
ACCESSION   DAOQFT010000026 DAOQFT010000000
VERSION     DAOQFT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4386_length_7888_cov_7.146176"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLR93_01225"
     CDS             <1..568
                     /locus_tag="PLR93_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNH25606.1"
                     /translation="HRVWERALRRASLPISYSQGFHPQPKISLAAALPLGFSSRAEVI
                     DVRLNEEIPTEEIHTRLKDNLPPDIKVTDVKSVDERLPALQTLVLSAAYDVHLTEPVA
                     GSELTRRVESLMQAESIIRERRGKTYDLRPLVEMLSVITQADGTVWLKMTLAAREGAT
                     GRPEEVLTVLEIEPETARVERTRLIFKE"
     gene            704..1687
                     /locus_tag="PLR93_01230"
     CDS             704..1687
                     /locus_tag="PLR93_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNH25607.1"
                     /translation="MALLAAIPMAFIPAFFFSWFLYWLDRYEKEPRWLLLMTFFWGGF
                     VAIIGTLIVASIFEVGFSFVLNDAVLEDIAGGSITAPLVEEFMKGFAVMLVFFMFRKE
                     FDSILDGIIYGGIAGLGFAATENVLYFLGQYNEAGWGGMFANFALRVGVFAWGHPFYT
                     AFTGLGFAVARNNKNILVKLIAPIIGYLLAVSAHAFHNTVLLFVSGLGSLAFVILVEW
                     GSWFIFLGFIAWMIRHEQGLLKKHLPEEVANGLISDAQYKTAVSFFQFGARMSALSGG
                     AYRATDHFYQYLGELAHKKEQLAKFGDERGNAAIIEKLRANIASLAAQAKT"
     gene            1774..1845
                     /locus_tag="PLR93_01235"
     tRNA            1774..1845
                     /locus_tag="PLR93_01235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1807..1809,aa:Arg,seq:ccg)
     gene            2039..>2484
                     /locus_tag="PLR93_01240"
     CDS             2039..>2484
                     /locus_tag="PLR93_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH25608.1"
                     /translation="MTVPEFVTATLPVPATMAFTATPITEMPSNETIAPTTTPIAGTT
                     TSEVNVRADTSTVSSSLGTVPAFNNVLVTGKDPSGIWYQILFNNGAGWVRADFIQLVD
                     TTVEIPVAVPETGSEAVSRGVVLRGVNVRSGPGQNFDSLGLLNQNDV"
     assembly_gap    2485..2584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2585..2834
                     /locus_tag="PLR93_01245"
     CDS             <2585..2834
                     /locus_tag="PLR93_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25609.1"
                     /translation="GDNEDWIGFSSEIKEVVIQVSCDAASITVELVQEGATNNDQVEC
                     GKPKIIKVTSWQEYLIKVTSKPVSESLVEYKLNIKIIK"
     gene            2859..4064
                     /locus_tag="PLR93_01250"
     CDS             2859..4064
                     /locus_tag="PLR93_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH25610.1"
                     /translation="MKRYLVVLSILVIIGVILTGCQTKEETSPLSAGLSELTGLVEMK
                     QAGQDAFTQAAADAVLEVNGQIQTGDDGRVRLDLSSGTIIRVAPSSFFTLTSNDEVEG
                     GLFTKIKLEAGKIFIVLNGGQTDVETPSGVASVRGSYLKVEVDPITKDIHITCLEGTC
                     SATNPNGEQIIFTNGQSVILFHQESDGSWKSPLLGDMTMEDFEEWLQNSNDSETKKYY
                     DEGVAKLLEATASPTEPPTEVPTDVPPTEVSSVNGQGETSNACSQLQEPIDGRALNNA
                     GKVNFAWSEQPNAQTYIITFIKEDGSTARIETSSNSAEFYIEVLPAGGNYQWFVTAYG
                     ADGKEICKSNSANFSKPQAEPTEKPKATKDPNAEEPASGTAGCPCDPYDYLNYSDCVG
                     SHMDVCSIP"
     gene            4092..5993
                     /locus_tag="PLR93_01255"
     CDS             4092..5993
                     /locus_tag="PLR93_01255"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH25611.1"
                     /translation="MSKNNNPLNLRLILVLGTAALLLIVQVLGLFSTITTPIERIEYS
                     MQDTFVRLRGAEKPSGEIVIVAIDDQSFNWTGLQWPWPRSYFAEIVDQINKGGGKVVG
                     MDLILFEPDEKQPENDLVLAKALGESPAAVAVMQINKTGGEGYATETLSQPLSAYRDV
                     LDGIGTTDVKRDEDAIVRNVSAYSIYQGQVYPHWAFELAKLYLNAEQPIFGNSSVKFN
                     GQNIPLRSNELPIYFAGPAGTYPTYSASDVHDGITLEQNPDAFRGKIVLIGATTITLQ
                     DIYPTPFSATDPTPGVEVVANTVDTIINGKFITYAPPWLALLLIIVAAGMAYLITLSK
                     RPSFTLTALGISILLYLVAAYFVFSSQRYILPTIAPTLMLFLGVILPTLEQAVSQEQE
                     KRRVRNLFSRFISPEMVDQMMKTQDINSLNKRADLSIIFSDIRGFTTLSEKMTPEGVV
                     ALLNPYLEAMSQVIYRHGGTVDKYEGDAIIAFFGEPVPYKDHAVRALRASMDMRKALA
                     ELRDKWAKEGRPSQIEMGVGINSGEVFVGLLGSAQRINYTVIGDNANLASRLQDLTKN
                     YAWPILISESTYQQVKDEFDCELADAVTVKGKTKAVNVYKVIGEKGAPESEQLQAWTS
                     DQVKPKHTS"
     gene            6165..6812
                     /locus_tag="PLR93_01260"
     CDS             6165..6812
                     /locus_tag="PLR93_01260"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNH25612.1"
                     /translation="MAILQKARAHMNARKIPDIIIGRLPVYLRALQRMADMGLKTTSS
                     QELGENVGISAAQIRKDISQFGEFGKQGTGYSIEYLINKLREILKVDRIWDVIVVGAG
                     DMGHAVANYQGFMNRGFHVVAIFDSDKSKVGQKIGNFTVEDTEVMHERIKELGVKLAM
                     LTVPAAAAQSVADQLVQAGVRAILNYAPISLNVPGNVKVQYIDPSTHLQRMTYYL"
     gene            6860..7525
                     /locus_tag="PLR93_01265"
     CDS             6860..7525
                     /locus_tag="PLR93_01265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH25613.1"
                     /translation="MSKIWNAAEYDVSRRRLIPCYDLFYGTAVELASRTVKDKNPSIL
                     DIGAGTGLLSEHVLQKFPTANLALLDESAEMLAKAQIRLEQYKPKVFVQAMTSALPSE
                     KFHAVISSLAIHHLSDKDKKGLFKRVFDILEPGGIFINAEQILGETPWQQSLFEETHL
                     DGARALGSSEEEIQAAVERMSYDKCSPLQEQILWLKEIGFENAASFFHSFRFAVYAGW
                     KPK"
     gene            complement(7571..>7888)
                     /locus_tag="PLR93_01270"
     CDS             complement(7571..>7888)
                     /locus_tag="PLR93_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNH25614.1"
                     /translation="VTGLPRPVTLTTGEIVEALQEPLKQIVESGRKVLEKTPPELVSD
                     IIDRGVALCGGGALLRGIDKLLTKSLGIPAYLVDNPLTCVVQGSAKAFGMLNVIRRNL
                     PQV"
BASE COUNT         2145 a         1777 c         1812 g         2054 t
ORIGIN      
        1 gcaccgagta tgggaacggg cattacgccg tgccagtctg cccatttcat actcacaagg
       61 ctttcacccc caacccaaga tcagcctagc tgctgcattg ccgcttgggt tttcctcccg
      121 tgctgaagtg atagacgtgc ggttaaacga agaaatccca accgaagaaa tccatactcg
      181 gttgaaggat aatcttccac ccgatattaa agtgacagat gtgaagtcag tggatgaacg
      241 tttgcccgct ttgcaaacac tggtgttatc agcggcgtat gatgtccatt tgacggaacc
      301 agttgccggg tccgaactga cgcgtagggt tgagtctctc atgcaggctg aatctattat
      361 ccgagaacgt cgtggcaaaa cctatgatct gcgtccgttg gttgaaatgc taagtgtgat
      421 cacgcaggca gatggcaccg tttggttgaa aatgactcta gctgctcgcg aaggcgcaac
      481 cgggcgccca gaagaagtat tgactgtgct tgagattgaa ccggaaacgg cgcgggtaga
      541 gcgcactcgc ctaattttca aagaataaac aaaacgtctg gcttagccag acgtttgatt
      601 ttagataagc gttttgaaat acccccttaa gttctattga tttttagccc agcatcagta
      661 aaattaatca gatatctaat ttcaactcac tggaggaatt tttatggcac tactagctgc
      721 aattccaatg gcattcatcc cagcattctt tttctcgtgg ttcttatatt ggctcgaccg
      781 ttacgaaaaa gaaccgcgct ggctgctcct aatgaccttt ttctggggtg gctttgttgc
      841 cattatcggc acattgatcg ttgcttcgat ttttgaggtc ggtttcagct ttgtgctgaa
      901 cgacgctgtc cttgaagata ttgctggcgg ctccattact gctccattag tcgaagaatt
      961 catgaagggc tttgccgtaa tgctggtctt cttcatgttc cgcaaagagt ttgactccat
     1021 cctcgacggc attatttatg gtggcattgc agggctcggc ttcgcagcaa ctgaaaatgt
     1081 tttgtatttt ctaggtcaat ataatgaagc gggctggggc ggcatgttcg caaactttgc
     1141 attgcgcgtg ggcgtgtttg catggggtca cccattctat actgccttca ccggtcttgg
     1201 ttttgctgta gcccgtaata acaaaaacat cttggtgaaa cttattgcac caatcattgg
     1261 ttatctgctt gctgtttcgg cgcatgcgtt ccacaatact gttttgctct tcgtgagcgg
     1321 attgggaagc ctagcctttg tcattttggt tgaatgggga agctggttca tcttccttgg
     1381 cttcattgct tggatgatac gtcatgaaca aggtttattg aagaagcatc tacctgaaga
     1441 agtagcaaat ggattaattt ctgatgcaca gtataaaaca gccgtctctt tcttccagtt
     1501 tggggcacgg atgtccgctc tgagtggcgg cgcttatcgc gctaccgatc acttctatca
     1561 ataccttggc gaattggctc acaaaaaaga acaactggca aaatttggtg acgaacgtgg
     1621 aaacgccgca atcatagaaa agctacgggc gaacattgcc agccttgccg cgcaggcgaa
     1681 gacctaagac atttcgcttc atctctaaat gtagggctgg cggctgctcc gtcagccctt
     1741 ttctacgcct atggtaaaat tcgcttcgct caagccccca tagctcagtg gacagagcga
     1801 cggcctccgg agccgtagag tgcagttcga gtctgcatgg gggtgcctca aaacatccgc
     1861 aaagaagcgg atgtttttct atttatcccc ccaaatgggt gattaagaac ccctttgtaa
     1921 caattactac cacccatttt ttagatacaa ttatcctgtg aacctaaaac attcaagtgc
     1981 cctttttatc atcattcttt tgtgcacagg gtgcgacgca tctttatcaa cagcaactgt
     2041 gactgtacct gagtttgtaa ctgcgacatt gcccgttcca gccacaatgg cgtttaccgc
     2101 tactccaata acagaaatgc cgtcaaatga aacaattgcg cctacaacta cacccattgc
     2161 tggaacgacc acatcagagg tcaatgttcg tgcagataca tctacagtta gttcgtcttt
     2221 aggcacggtc cctgccttta ataatgttct ggtaacaggc aaagacccaa gtgggatttg
     2281 gtatcaaatc ttgtttaaca atggtgcggg gtgggttcga gccgatttta ttcagttagt
     2341 agacacaact gttgaaatcc ctgttgccgt accagaaaca ggaagtgagg ctgtaagtag
     2401 aggcgtagtt ttgagaggcg ttaacgtacg cagcggacca ggtcaaaact ttgattcgtt
     2461 agggttatta aatcaaaatg atgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnntggaga taatgaagac tggataggct tttcgtccga aataaaagaa gttgtaattc
     2641 aagtttcatg cgatgccgct agcattacag tagaattagt tcaagaaggt gcaactaata
     2701 atgaccaagt agaatgtgga aagcctaaaa tcattaaagt cacttcatgg caggaatatc
     2761 tcattaaagt tacatcgaag cctgtcagtg aaagcctagt cgaatacaaa ttgaacataa
     2821 aaattatcaa ataaagcacc aaacgaacgg agagagaaat gaaaagatat cttgtggttt
     2881 tatcaatact cgtcatcata ggggtgattc ttacgggatg ccagaccaaa gaggaaacat
     2941 cccctctttc tgctgggctt agtgaattaa cgggacttgt ggaaatgaag caagctgggc
     3001 aagatgcctt tactcaagcc gcagcggatg ccgtacttga agtgaatggg cagattcaaa
     3061 ctggagacga tgggcgcgta cgacttgatc tttcttctgg aacgattatt cgtgtggcgc
     3121 catcttcatt tttcacccta acctcaaatg acgaagtaga aggcggtctg ttcaccaaaa
     3181 taaagctcga agcagggaaa attttcatcg tcctcaacgg tggtcaaact gacgttgaga
     3241 cgccttctgg tgtggcttcg gtgcgcggct catacttaaa agtagaagtg gaccctatca
     3301 ccaaagatat tcatattact tgtctcgaag gaacttgttc tgctactaac ccaaatggag
     3361 aacagatcat ttttaccaat gggcaatctg taattctttt ccatcaagaa tccgatggtt
     3421 catggaagtc tccattactt ggggatatga ccatggaaga ctttgaagaa tggctccaga
     3481 acagtaatga tagcgaaaca aagaaatact atgatgaagg agtagcaaag cttttagaag
     3541 ccactgcatc tcctacggaa ccgccaactg aagttccaac cgatgtaccg cctaccgaag
     3601 tcagttctgt caatggacaa ggtgaaacaa gcaatgcgtg ttcgcagtta caagagccaa
     3661 tagatggccg tgcgctcaat aatgctggaa aagtcaattt tgcatggagt gaacaaccga
     3721 acgcgcaaac ctacattatt acatttataa aagaagatgg cagcaccgct cgaattgaaa
     3781 ccagttcaaa tagcgcagaa ttttacattg aggttttgcc tgctggtgga aactatcaat
     3841 ggtttgtcac agcctacggc gcagacggaa aagaaatttg taaatcaaat agtgccaatt
     3901 tctcaaaacc gcaagcagaa ccaactgaaa agccaaaagc aaccaaagat ccaaatgcag
     3961 aagagcctgc tagtggtacc gctggctgtc cttgtgaccc ctatgattac ttgaactatt
     4021 ccgattgtgt tggctctcac atggatgttt gtagtattcc ttaatcatct caagccatca
     4081 aaggataaga catgtcaaaa aataataatc ccttaaactt acgccttatt ctggtacttg
     4141 ggacagctgc actactctta attgttcaag ttctgggatt atttagcacc atcaccacac
     4201 ccattgagcg gattgaatac tccatgcagg atacatttgt gcgcctgcgt ggagcggaaa
     4261 aaccctctgg tgagattgtc attgtagcaa ttgacgacca atcttttaac tggaccggct
     4321 tgcaatggcc ctggcctcgc tcctattttg cagagatcgt tgaccaaatc aataaaggcg
     4381 gcggcaaggt cgttggtatg gatttaatcc ttttcgagcc cgatgaaaag caacctgaaa
     4441 atgacctggt tctcgcaaaa gcacttggag agagccccgc cgcagttgct gtgatgcaga
     4501 tcaataaaac gggtggagaa ggctatgcaa ccgaaaccct gtctcagcca ttatcggcgt
     4561 accgtgatgt tctagatggc ataggcacaa cagatgtaaa acgcgatgaa gacgccatcg
     4621 tgcgaaatgt atcggcatac agtatctatc agggacaggt ttatccgcat tgggcatttg
     4681 aacttgcaaa gttatatttg aatgctgaac aaccaatatt tggaaattca agcgttaaat
     4741 ttaacggtca aaacattccg ttacggtcca atgaacttcc tatctatttt gcaggacctg
     4801 caggaaccta cccaacctat tctgcatcag acgttcacga tggcatcacg ttggaacaga
     4861 acccagatgc cttccggggc aaaattgtct tgattggcgc gacgacgatt accttacagg
     4921 atatttaccc aacccccttt tcggcgacag atccaacacc tggtgtggaa gtcgttgcga
     4981 acacggtaga tacaatcatc aatggcaagt tcattaccta tgccccacct tggttggcac
     5041 ttttgctaat aatcgtggcg gctggtatgg cataccttat cacgctttca aagcgcccgt
     5101 cttttacact caccgcttta ggcattagca ttcttctcta tttggttgct gcgtattttg
     5161 ttttctccag tcaacgttat atattgccaa ccattgcgcc aacccttatg cttttccttg
     5221 gcgttatcct tcccacatta gagcaggcag tttctcagga acaggaaaag cgccgtgttc
     5281 gtaatctatt cagccgtttc atttctccag aaatggttga ccagatgatg aagactcaag
     5341 atattaattc actcaacaaa cgtgcagatc tttccattat cttttcagac attcgcggct
     5401 tcacgacact ttctgaaaaa atgacgccgg aaggagtggt tgccctgctt aacccctacc
     5461 tcgaagccat gtcgcaggtc atttatagac atggcggcac cgtagataaa tacgaaggcg
     5521 atgccatcat tgcgttcttt ggcgaacccg ttccctataa agaccacgcg gttcgtgctc
     5581 tgcgcgcctc aatggatatg cgcaaagcat tggctgagct tcgagataaa tgggcaaaag
     5641 aaggtcgccc cagccagatc gaaatgggag taggcatcaa ctcgggcgag gtgtttgtag
     5701 ggctgcttgg ctcagcgcaa cgcatcaact atacggtcat tggcgataat gccaaccttg
     5761 cctctcgcct gcaagacctc accaaaaact acgcctggcc cattctcatc agtgaaagca
     5821 cctatcaaca agtcaaggat gaattcgatt gcgaacttgc agatgcagtc accgtcaaag
     5881 ggaaaaccaa ggcggtcaat gtatacaagg tcattggcga aaaaggcgct ccagaaagcg
     5941 aacaactaca agcctggaca agcgaccagg tgaagcccaa acacacatca tgacgcaaaa
     6001 acggagcgag aattctcgct ccgtttttgt accggctcag gcgtagacgc aggcttggtc
     6061 tgtcaaccag gcagaaatcc acgctggagg cggattttta atcccctgtt tatttgtgaa
     6121 agacttgtac ataattgtac aaaaagcgtt tttttggtat agttatggct atactacaaa
     6181 aggcgagggc tcatatgaat gcgaggaaga tacccgatat tatcatcgga agactcccgg
     6241 tgtatttgcg tgcgttacag cgcatggcag atatgggatt aaagaccacc tcatcgcagg
     6301 aattgggtga aaatgtgggc atttctgctg cccagatccg caaggatatc tctcaatttg
     6361 gcgagttcgg taagcaaggt acgggctatt ccatcgaata cctcatcaac aagctacgcg
     6421 agattctcaa agtggatcga atttgggatg tgatcgtagt cggtgcgggt gatatgggac
     6481 atgcagttgc caattatcaa gggtttatga accgtggctt tcatgtagta gcaattttcg
     6541 atagcgacaa atcgaaggtt gggcaaaaga ttggcaactt caccgtggaa gatacggaag
     6601 tgatgcatga gcgtatcaaa gaattaggcg tcaaacttgc gatgctgact gttccggcag
     6661 cggctgccca atctgtggca gatcaactgg tgcaagcagg cgtacgcgca attctcaact
     6721 atgcccccat tagtttaaat gtgcctggca atgtcaaagt gcaatatatt gacccatcca
     6781 cacatctaca aaggatgacg tattatttgt aaaaccagat gcccggtaca acaccgggca
     6841 ttttttatag gaacttacga tgagcaaaat ttggaatgcc gctgaatatg atgtctctcg
     6901 ccgtcgcctg atcccctgtt atgacttgtt ctatggcaca gcagttgaat tggcgtcacg
     6961 aactgtgaag gataagaatc cgtcgatcct tgatattggt gcaggaactg ggctgttgag
     7021 tgaacacgtg cttcagaagt tcccaacggc aaacctcgcc ttactggatg aatccgcaga
     7081 gatgcttgcc aaggctcaaa ttcggctgga acaatacaag cccaaggttt ttgttcaggc
     7141 aatgacatct gctttgccat ctgaaaaatt tcatgccgtt atttcatctc tcgcaattca
     7201 tcacttaagc gataaagata aaaagggttt attcaaacgg gttttcgata ttctggaacc
     7261 aggtggaatt ttcatcaacg cagaacaaat tttaggcgaa accccttggc aacaatcctt
     7321 gtttgaagag actcatctgg atggcgccag agccttaggc agcagcgaag aggagattca
     7381 agcagccgtt gaacgtatga gttatgacaa atgttcaccg cttcaagagc aaattttatg
     7441 gctcaaagaa attggatttg agaacgccgc ttcttttttt cattcgtttc gttttgccgt
     7501 atacgcaggc tggaaaccta aataattttt acaaaacaaa aacggcttcc aaattggaag
     7561 ccgttcattt ttacacctga ggtaaattcc tgcggatgac gttcagcatg ccgaaggctt
     7621 tggctgagcc ttgcaccaca caggtcaaag ggttatctac gagataagct ggaataccga
     7681 gtgacttggt gagcaactta tcaatgccgc gcagaagcgc tcccccgccg cataatgcca
     7741 ctccacgatc gataatatca gagacgagtt ctggcggcgt cttttcgaga accttacgcc
     7801 cagactcaac aatctgtttg agcggctctt gcagagcttc tacgatctcg ccggtggtca
     7861 atgtgactgg gcgtggcaga ccagtgac
//
LOCUS       DAOQFT010000027         7794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4474_length_7794_cov_5.625275, whole genome shotgun sequence.
ACCESSION   DAOQFT010000027 DAOQFT010000000
VERSION     DAOQFT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4474_length_7794_cov_5.625275"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLR93_01275"
     CDS             <1..1062
                     /locus_tag="PLR93_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25615.1"
                     /translation="LVKIAGRWFPRALLVDINEGQLNLVEAVLDMAGGEPLTTADLMK
                     DTELPTGVNPKLVEFSLNYAMQYDDRFDEVGPAGQVLWCLRRLEPDFVRDVPPPLRYM
                     DIPHDRSFLSPEMLALEAQLDDELTETDAADRDDVSSVTITLIYPHLRAGTLPVSPRT
                     RKLFPTAYETPRVRFTLVDGKTKQRIPAWVVLEHGYVFGLREWYKAHQLIPGSLVQVR
                     KGENPGEVIVEAKSQRSAKDWVRTVMVGSDGGFVFATLKQPITAEFNDRMVVHVPDFK
                     ALDPIWEKKRSFEDLLLMVMRELAKSNPQGHVHAQELYAGVNLVRRVPPAPIFAALAS
                     NPVFKHVGDLHFRLDEEVN"
     gene            1062..1496
                     /locus_tag="PLR93_01280"
     CDS             1062..1496
                     /locus_tag="PLR93_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25616.1"
                     /translation="MSLLKPTVKTPFHIDFEWWKQNEGDWHIFLRSFLCAEHQEAFAN
                     VEEGEMIDWIDPQTAEVKPVDGVQHALISHCAQLPDFVNQRTAIVEAIFRLFLANGNI
                     PMSAEDLSKKLGKPADTILRTIAGPRVYRGLRPMLNQPTETM"
     gene            1521..1593
                     /locus_tag="PLR93_01285"
     tRNA            1521..1593
                     /locus_tag="PLR93_01285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1554..1556,aa:Arg,seq:tct)
     gene            1628..2011
                     /locus_tag="PLR93_01290"
     CDS             1628..2011
                     /locus_tag="PLR93_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH25617.1"
                     /translation="MPTVIRKTRETLMEVRRGVLHAVSWQVKSSVWDPPTDLYETEEA
                     YIVRMEIAGMREENFEVSLLNDTLYITGFRHDTPEKRAVHQMEIRSGKFASMVRLPEP
                     VNVEKSLAEYQDGFLTIILPKETNE"
     gene            2024..4603
                     /gene="lon"
                     /locus_tag="PLR93_01295"
     CDS             2024..4603
                     /gene="lon"
                     /locus_tag="PLR93_01295"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNH25618.1"
                     /translation="MPAQKWQSGLQNFLQMMGENEDEFSNQFMNSFFSQLQKKDDDSN
                     SSQSNGDNEKMSLPDVLPILPLRGVVVYPNTAVPLTVGQARSIKLVDDVVSGDKLVGL
                     VAALNPEQEMPGPNELYRVGTVATVHRLLRAPDGTVRLLVQGMERFRLGEFVEEEPYL
                     KAKIIRAPELDEKNIETDALARNARDQFQAITQMIPSFPEELSSSILSLEDPLQTVYT
                     IANFQRIDLKDAQEILEIDSVYNKLKKLIGLLVREAEVLQIGQKIQNEARGEIEKVQR
                     DYFLREQMKAIQRELGERDEQSEEVDEFRKKIDEAKMPEEADKQARRELDRLSRLPTA
                     AAEYGVIRTYLDTMVSLPWSAGTEDNLDIAHARQVLNQDHYGLEDVKDRILEFLAIRK
                     LRLDRANDKKEESKDQIRHEREGVILCFVGPPGVGKTSLGQSIARAMGRKFIRASLGG
                     VRDEAEIRGHRRTYIGAMPGRILQSLRRVGSKNPVFMLDEVDKLTNDFHGDPSSALLE
                     VLDPEQNGEFRDNYLEVAYDLSQVFFIATANTLEYIPGPLRDRMEIIYLSGYTETEKT
                     QIARGYLIPRQIRENSLRKSEIAFNDEALKLIIRQYTREAGVRNLERKIGAVCRKVGT
                     RIAEGKKKKYNINPKVVEEFLDNPIFLGPEELNKRTSIPGVVPGLAWTSFGGDILLIE
                     ATSMPGGRGFQITGSIGNVMQESARAALSYVRSRSNSFGIAHEYFEKHDIHMHVPAGA
                     QPKDGPSAGVTMATSLVSLITGRKVSPQVGMTGEITLRGQVLPIGGVKEKVLAAHRSG
                     LGTVILPKQNEKDLDDVPDEIRKSMKFVFAETVEDVINSALEKPKQKKKTTKKETKLR
                     KRK"
     gene            4610..4966
                     /locus_tag="PLR93_01300"
     CDS             4610..4966
                     /locus_tag="PLR93_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25619.1"
                     /translation="MPKVLLAEDDFTMVSLLKTLLKMEGYEVIALDADADVIAAVRSM
                     KPDVLLLDVHLFSQSGLDILDTLRRSKDTSKVRVLMSSGSNVREECMMRGANGFLMKP
                     YMPDDLFTQLKQVLSV"
     gene            4963..5394
                     /locus_tag="PLR93_01305"
     CDS             4963..5394
                     /locus_tag="PLR93_01305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HNH25620.1"
                     /translation="MTQKFHIREFIFDQDYEPVLRLWQNIESGMQVGRSDSPEEIKRK
                     VERDPDLFLLAEVEGTIIGTVIGGFDGRRGMVYHLAVTQKYRRQGVAATLLAEVEKRL
                     QAKGCVKVYLLVLEDNTSAIRLYEERGWKHQKQDLIFSKEF"
     gene            5395..5889
                     /locus_tag="PLR93_01310"
     CDS             5395..5889
                     /locus_tag="PLR93_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNH25621.1"
                     /translation="MTMRFGIITLSDRSSRGEREDLSGPALASFLQTENCSVVKQLIL
                     PDDESALRHTLTEWADSDEFDVILTTGSTGFAPRDIAPEATKAVIQKEAPGLAELMRT
                     ESLKKTKHAALSRAMAGIRKHTIIINLPGSPKGAVENLGFVFPLLQHAVQLLRDDPDS
                     EKSH"
     gene            6069..6506
                     /locus_tag="PLR93_01315"
     CDS             6069..6506
                     /locus_tag="PLR93_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH25622.1"
                     /translation="MSTVRDMIRKKGSEVFSISPDATVFEALSQMAKYNTGAMMVVRN
                     GKVEGIVSERDCVRKMDLEGRSAKTAKVNEIMTSKVIYVEASQQLEECMALMIDKNIR
                     HLPVYDGNELLGLISVRDVLREVIDVQKFMISQLEHYITGGGR"
     gene            complement(6551..7498)
                     /locus_tag="PLR93_01320"
     CDS             complement(6551..7498)
                     /locus_tag="PLR93_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNH25623.1"
                     /translation="MTVTINDIRQAAQRIKPYIHHTPVMTNESLNQRVGVQVFMKCEN
                     LQKVGAFKFRGASNAVWSLTDKEASSGVVTHSSGNHAQALALAAKMRGIPAYIVMPSN
                     APQVKKNAVAGYGGQITFCEPTLAARESTMESIRQRTGATVVHPYDNEHVIAGQGTAV
                     LELLEDIPDLDVIIAPVGGGGLLSGTSIAATEMKKGIRVIAAEPEMADDTFRSLQAGE
                     IIPSMNPKTVADGLLTSLGSLTFPIIKERVEQIVTVSEAGILDAMKFIWERAKIVIEP
                     SAATVIAVLWEKKIDLTGKKVGVILSGGNVDLEKLPWQK"
     gene            complement(7498..>7794)
                     /locus_tag="PLR93_01325"
     CDS             complement(7498..>7794)
                     /locus_tag="PLR93_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH25624.1"
                     /translation="ALPSHAIFINVGRGSTVDEPALLKVLQENKIAGAVLDVFEQEPL
                     PSEHPFWDAPNLSITFHTSAPSLPEDIAQVFIENYRLYSEGKPLKHQVDFERGY"
BASE COUNT         2036 a         1810 c         1859 g         2089 t
ORIGIN      
        1 ttggtgaaaa ttgcaggacg ttggttccca cgcgctttgt tagtggatat caacgaaggt
       61 caattaaatc ttgtagaagc tgttttagat atggcaggcg gtgagcctct aaccactgct
      121 gatttgatga aagatactga actgccaacg ggcgtaaacc ctaagctggt tgagttttct
      181 ttgaactatg cgatgcagta cgatgatcgc tttgacgaag tgggtcccgc cgggcaggta
      241 ctttggtgct tacgccgcct cgaaccggac tttgtgcggg atgtccctcc gccgcttcgc
      301 tacatggaca tccctcatga ccgcagcttc ttatctcctg aaatgctcgc gttggaagct
      361 caactcgacg atgagctaac tgagacagat gctgcagatc gcgatgacgt ttcttccgtt
      421 accatcacct taatctaccc acaccttcgt gccgggacgt tacccgtctc accgcggacc
      481 cgcaaactgt tcccaaccgc gtatgagacg ccacgtgtgc gttttaccct tgtagatggt
      541 aaaaccaaac aacgcattcc ggcttgggtt gttttggaac acggctatgt ctttggttta
      601 cgtgaatggt acaaggcgca tcaattaatc cctggttcgt tggttcaggt gcgcaagggt
      661 gagaaccctg gcgaagtgat tgtggaagca aaaagccagc gctctgcaaa ggattgggtg
      721 cgcaccgtga tggttggttc agatggtggt tttgtctttg ctaccctcaa acagcccatc
      781 actgcggaat ttaatgaccg tatggtggtg catgtccctg atttcaaggc gctcgacccg
      841 atctgggaaa agaagcgttc ttttgaggat ttactcttaa tggtcatgcg tgaattagct
      901 aaatcaaacc cgcaaggaca cgtccatgcg caggaattgt atgctggcgt gaatcttgtt
      961 cgccgtgtgc cgcctgcacc gatctttgct gctttggcat cgaaccccgt tttcaagcat
     1021 gttggcgatt tgcacttccg cctcgatgaa gaggtgaact gatgagtctc ttaaagccga
     1081 cagtaaaaac cccgtttcac attgactttg agtggtggaa gcaaaatgaa ggggactggc
     1141 atattttctt gcgctccttc ctttgtgccg aacatcaaga agcgtttgcc aatgtggaag
     1201 aaggcgaaat gatcgactgg atcgacccgc aaactgccga ggttaaaccg gtggatgggg
     1261 tccaacacgc tttgatcagc cactgtgctc agttgcccga tttcgtcaat cagcggacgg
     1321 cgattgttga ggcaattttc cgcttgttcc ttgctaacgg aaatattccc atgtctgctg
     1381 aagatttaag caagaaattg gggaaacctg ccgacaccat cctgagaact attgcgggtc
     1441 cgcgggtgta ccgtggttta cgtcctatgt tgaaccagcc cactgagacc atgtagtcgg
     1501 gtaaaattaa agcaacacaa gccctcgtag ctcaatggac agagcagcag ccttctaagc
     1561 tgttggttgg gagttcgatt ctctccgggg gcgctcgaaa gctggcaaga tattaaggag
     1621 tcgtcctatg cctactgtga ttcgaaaaac acgcgaaaca cttatggagg tcaggcgcgg
     1681 cgtcctgcat gcagtgagtt ggcaggtcaa atcaagcgtc tgggatcctc caactgacct
     1741 ctatgagacc gaagaagcct atattgtacg catggaaatt gcaggcatgc gcgaagagaa
     1801 cttcgaggtt tcactgttga acgatacgct ttacatcact ggttttcggc acgacacccc
     1861 agaaaaacgc gcagttcatc aaatggaaat ccgctctgga aagtttgctt caatggtgcg
     1921 gcttcctgag ccagtcaatg ttgaaaagtc actggcagaa tatcaagatg gttttctaac
     1981 catcatcctc ccaaaagaaa cgaacgaata aggttattac tcaatgcctg ctcaaaaatg
     2041 gcaatctggc ttgcaaaatt ttcttcaaat gatgggcgaa aacgaagatg agttttcgaa
     2101 ccagttcatg aactctttct tttcacagct tcagaagaaa gatgatgact caaatagctc
     2161 gcaatcgaat ggcgacaatg aaaagatgag tcttcctgat gtgttgccca tcctaccttt
     2221 gcgtggtgta gtggtatatc caaataccgc cgtcccgttg acggtggggc aggctcgttc
     2281 catcaaattg gtggatgacg ttgtctctgg cgataagctt gtcggtttgg tggcggctct
     2341 taacccggaa caggaaatgc ccggacccaa tgaactatat cgagtcggga cggttgctac
     2401 ggtccatcgt ttgctgcgcg caccagatgg aactgtccgc ttgttggtac aaggcatgga
     2461 gcgttttcgt ctgggcgaat ttgttgaaga agaaccttat ctcaaggcga agattattcg
     2521 tgcaccagag cttgatgaaa aaaatatcga aacagatgcc ttggctcgca atgcacgtga
     2581 ccagttccaa gcaattactc aaatgattcc ctcttttcca gaggaattat caagttctat
     2641 cctttcattg gaagatccgc tccaaactgt ttacaccatt gcgaactttc aacgcattga
     2701 cttgaaagac gctcaagaga ttcttgaaat cgactcagtc tacaataagc tcaagaaact
     2761 aatcggcttg ttggtgagag aagccgaggt tttacagatt gggcaaaaga tccagaatga
     2821 ggcgcgtgga gagatcgaaa aagttcaacg cgactacttc ctccgcgagc agatgaaagc
     2881 aattcagcgg gaactcggcg aacgtgacga gcaatcggag gaagttgatg aattccggaa
     2941 aaagatcgac gaggcgaaga tgcctgaaga ggctgataaa caagcccggc gtgagttgga
     3001 ccggctctct cgtcttccta ctgctgccgc agaatatggt gttattcgca cctatttgga
     3061 cacgatggtt tcgttgcctt ggtctgcagg tacagaagac aatcttgata tcgcccatgc
     3121 tcgccaggta ttgaaccaag accattatgg tttggaagat gtcaaggacc gcattcttga
     3181 attccttgcc attcgtaaat tgcgattgga tcgtgccaac gataaaaaag aagaatcgaa
     3241 agaccagatt cgccatgaac gcgaaggtgt gattttgtgt tttgtgggtc ctcctggggt
     3301 tggcaaaact tctttgggac aatccattgc ccgtgcgatg ggacggaagt tcatccgcgc
     3361 atcattaggc ggtgtgcgtg atgaagctga gattcgtggg catcgacgta cctatatcgg
     3421 tgccatgccg ggtcgcattt tgcaatcgct gaggcgagta ggctccaaga acccggtctt
     3481 tatgcttgat gaagtcgata aactcacgaa cgactttcac ggcgacccat cctctgcctt
     3541 gctggaggtg ctcgaccctg aacagaatgg tgagttccgc gataattatc ttgaagtagc
     3601 atatgacctt tcgcaggtgt tctttattgc cactgcaaat acgttggaat atatccccgg
     3661 tccgctgcgt gaccgcatgg agattattta tctttcaggt tataccgaaa ctgagaagac
     3721 tcaaattgca cgcggctatc tcattccgcg ccagattcgt gagaatagtt tacgtaaatc
     3781 tgaaattgct ttcaatgacg aagccttgaa gttgattatc cgccaatata cgcgtgaagc
     3841 tggggttcgt aatttagaac gcaaaatcgg cgctgtttgt cgtaaggttg gtactcgcat
     3901 tgcagaaggc aagaaaaaga aatacaacat caacccaaaa gtagtggaag aatttctcga
     3961 taaccccatc ttcctcggac ctgaagagtt gaataaacgc acttcgattc ccggtgttgt
     4021 gcccggtttg gcgtggacct cttttggggg tgacattctt ttgattgaag ccacttccat
     4081 gccaggaggt cgcggtttcc agattacagg ctcgattgga aatgttatgc aagaatctgc
     4141 gcgtgcagct ctttcatacg tccgctcgcg ttcgaactct tttgggatcg cgcatgaata
     4201 ttttgagaag catgatattc atatgcacgt cccagcaggg gctcaaccca aagacggtcc
     4261 ctccgctggt gtgacgatgg caacctcact cgtgtcactc attactgggc gaaaggtcag
     4321 cccgcaggtt ggaatgaccg gtgagattac cttgcgaggt caagtcttgc caattggcgg
     4381 cgtcaaagaa aaggtgcttg ctgctcaccg tagcggactt ggcaccgtca tcctgccaaa
     4441 acagaacgaa aaagatctag acgatgtgcc agacgagatt cgtaaatcta tgaaatttgt
     4501 ttttgctgaa accgtcgaag atgtcatcaa ttcggcgttg gaaaaaccga aacagaaaaa
     4561 gaagacgact aagaaagaaa caaaacttcg aaaaagaaag taacccagaa tgccaaaagt
     4621 actcctcgcc gaagatgatt tcaccatggt ttccttgctc aaaactctcc tgaaaatgga
     4681 gggctatgag gttattgccc ttgatgcgga tgcggatgtg attgcagccg tccgttctat
     4741 gaaaccggac gttctcctgt tagatgtcca tttgttcagc cagagcgggt tagatatcct
     4801 tgataccttg cgtcgctcaa aagatactag caaagttcgc gtgttgatgt cttctggttc
     4861 aaacgtccgc gaagaatgta tgatgcgcgg cgcgaatggt tttcttatga aaccctatat
     4921 gccagatgac ctcttcacac agctcaaaca agtcttatcg gtatgaccca aaagtttcat
     4981 atacgagagt tcatcttcga tcaagattat gagcccgtat tgaggctttg gcaaaatatt
     5041 gagtctggca tgcaagttgg tcgttctgac tctccagaag aaatcaaacg taaagtagag
     5101 cgcgaccctg atttgttttt gttggctgaa gtagaaggca ccatcatcgg aacagtcatt
     5161 ggcggtttcg acggcagacg cggcatggtt tatcaccttg cggttactca aaaatatcgc
     5221 aggcaaggtg ttgctgccac tcttcttgcc gaagtcgaaa aacgtttgca agccaaaggt
     5281 tgtgtcaagg tctatctgct agttcttgaa gacaatacat ctgcgattcg tctttacgaa
     5341 gaacgtggtt ggaaacacca aaaacaagac cttatttttt ctaaagagtt ttaaatgaca
     5401 atgcgatttg gaatcatcac cctctcagat cgttcttcac gcggtgaacg agaagacctc
     5461 tccggacctg ctctcgcctc ttttcttcaa accgaaaatt gttcggtcgt aaaacaactg
     5521 attctccctg acgatgagtc agcgcttcgt cacaccctta ctgagtgggc agacagtgac
     5581 gaattcgatg tgatcctaac aacaggcagc accggttttg ctccgcgaga tattgcccca
     5641 gaagcaacaa aggctgttat ccaaaaagaa gcccccggtc tggctgaatt gatgcgcact
     5701 gaatctctca aaaagaccaa acacgccgct ttatctcgtg ctatggcggg tattcgtaaa
     5761 cacaccatca ttatcaactt gcccggtagt cctaaaggtg ctgttgaaaa tttaggtttt
     5821 gtttttcctc ttctgcaaca cgcagttcaa ttgttgcgtg atgacccaga ctctgagaag
     5881 tcacactaaa tagaaaaaac agcccacaaa atcaaattct tcatttgtaa acaaataaga
     5941 gtcaaaagtt gatgttcgct gcttttaaat attgcgctaa tcttcacata gggtatattt
     6001 ttttcttgtc attcctacaa ttcttgtcta tactgtagga aattcaatac ccgccaagga
     6061 gataaccgat gtccactgtg cgcgatatga tccgtaaaaa aggaagcgaa gtgttctcca
     6121 tttcacccga tgcgactgtt tttgaggcgc taagccaaat ggcaaagtac aacactggcg
     6181 ccatgatggt agtccgcaac gggaaggttg aaggaattgt ttcagaacga gattgtgtac
     6241 gaaagatgga cctagagggg agatctgcta agacagccaa ggtcaacgag attatgacca
     6301 gcaaggttat ttatgtagag gcgagccagc aactagaaga gtgcatggca ctgatgatcg
     6361 ataagaacat tcgccatttg ccagtttatg atggcaatga acttctcggg ctgatttcgg
     6421 tccgcgatgt gttgagagag gtcatcgatg tacaaaaatt tatgatttct caattagaac
     6481 attacattac aggtggggga aggtagttat gaaatcaaac accctgcatt tgcagggtgt
     6541 ttttcttatt ttacttttgc caaggcaact tttccaaatc tacattccca ccgcttagaa
     6601 tcaccccaac tttttttcct gttaaatcaa ttttcttttc ccataaaacc gcaatgaccg
     6661 tcgccgctga aggttcgatc acgatcttag cccgctccca tataaatttc atcgcatcca
     6721 aaatccctgc ttcacttact gtgacaattt gctcgacgcg ctctttaatg atggggaatg
     6781 ttagagatcc taatgacgtt aataacccat ccgccactgt tttgggattc atcgacggaa
     6841 taatttcacc ggcttgtagt gaacgaaatg tgtcatctgc catctcaggc tcagccgcaa
     6901 tcacccgaat cccttttttc atttctgttg ctgcaatgga tgtgccgctt aacaatccac
     6961 cgccaccaac aggtgcgata atcacatcca aatctggaat atcttccagc agttccaata
     7021 ctgctgtccc ttgacctgca atgacgtgtt cattgtcata tggatgaacc acggtcgcgc
     7081 ccgtgcgttg cctgatgctc tccatagtgc tttctctcgc tgcgagcgtt ggctcgcaaa
     7141 atgtaatttg tccgccatat cctgccacag catttttctt cacctgtggt gcattactcg
     7201 gcatgacgat atatgcagga ataccacgca tctttgcagc caaagccaac gcctgtgcat
     7261 gattcccaga tgagtgagtg accactccgc ttgacgcttc tttatctgtc aatgaccaga
     7321 cggcatttga tgccccacga aatttgaatg ccccaacttt ttgcaggttc tcgcatttca
     7381 taaaaacttg tacaccaact ctttggttta aactttcatt ggtcattact ggtgtatggt
     7441 gaatgtatgg cttgatgcgt tgcgctgctt gtcgaatatc atttattgtg acagtcatta
     7501 gtatcctcgt tcaaaatcaa cttggtgctt caatggcttt ccttcactat aaagcctgta
     7561 gttttctata aaaacttggg caatatcttc cggtaaactc ggtgcggatg tatggaaagt
     7621 tatgctcaga ttcggggcgt cccagaatgg atgctcgctt ggcaaaggct cctgttcgaa
     7681 aacatccaaa actgcaccag cgattttatt ttcttgtaga actttcaaga gcgcaggttc
     7741 atctactgtt gagccgcgcc ccacgtttat gaatatggca tgggaaggta aagc
//
LOCUS       DAOQFT010000028         7762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4510_length_7762_cov_6.450370, whole genome shotgun sequence.
ACCESSION   DAOQFT010000028 DAOQFT010000000
VERSION     DAOQFT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4510_length_7762_cov_6.450370"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..864)
                     /locus_tag="PLR93_01330"
     CDS             complement(130..864)
                     /locus_tag="PLR93_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH25625.1"
                     /translation="MKSFRADLHVHTVLSPCAEVEMIPPLIVQEALEQHIDIIAITDH
                     NASANVGSVQKAAEGTGLTVLPGMEIQSREDVHLLCLFASLADLEAWQHQVDLALPDE
                     LNPAEHLGEQFVVDEQGEYIRTEPRLLLTATKFSIEEIIELVIKLNGIVIPAHVDRPS
                     FGLFPTLGFLADWWRFPALEISRHITREMLYQKFPSSKRLPVIQSGDVHRLSEFLGST
                     IFELEAPTLTEIQMALRAENSRKLQM"
     gene            complement(867..1205)
                     /locus_tag="PLR93_01335"
     CDS             complement(867..1205)
                     /locus_tag="PLR93_01335"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNH25626.1"
                     /translation="MNLQTIIEKLQLNILTEPRDFSSITPKGGYTSDLLSCVMAGAKN
                     GNIWVTLQAHMNIVAVAAMLDVSAVIITENAQPDEATITKANEQGVILLSTPQASYEV
                     CGALWDLNLR"
     gene            complement(1208..2209)
                     /locus_tag="PLR93_01340"
     CDS             complement(1208..2209)
                     /locus_tag="PLR93_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH25627.1"
                     /translation="MDPTRQPTEQEKFGRHRPLTDRDAHEINRIEELSYDLKVREVMI
                     KNVITTTSDVPLSSALELLRVNRISGLPVVSDGTLIGVISLEDIVRALQTNGLSLPVK
                     AYMSTTLVTVKTTDSVVEAIKTFSEKRLGRLPVVDEHQKLEGIITKGDITRGILLAVE
                     RDYKEEEVRRYRASHLFDDIVSDRTTLVLRYNIKVGDFMHGGNASSNIKRALLRLGAD
                     AQLARRCGIAAYEAEMNLIIHTTNGGVLKLEVEPHRISLITTDDGPGIPDVQKAQQAG
                     YSTATDKVREMGFGAGMGLVNIKRCVDSMELESTTGKGTKLTMRINVPTESLNSKKG"
     gene            complement(2196..2561)
                     /locus_tag="PLR93_01345"
     CDS             complement(2196..2561)
                     /locus_tag="PLR93_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25628.1"
                     /translation="MNMNLKDIIVIAEGELLTPELNTDVNIHGGCCADLMSDVLAYSR
                     PRAVLLTGLTNPQVVRTAQMADFRAIVFIRGKRPQQETIDIANQENIPLITSPMGMFE
                     LCGKLHDSGLKSYEGNGSD"
     gene            complement(2741..3019)
                     /locus_tag="PLR93_01350"
     CDS             complement(2741..3019)
                     /locus_tag="PLR93_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25629.1"
                     /translation="MSEERAKPKAFATLCGRIFRDSDPQYFPFAEYKGKKILLCTEAC
                     LGAFLADPDIFCKVHRNSDKTAARMKKEIEHLATLWSQYASERPTEEK"
     gene            complement(3016..4734)
                     /locus_tag="PLR93_01355"
     CDS             complement(3016..4734)
                     /locus_tag="PLR93_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNH25630.1"
                     /translation="MTSLKWWQTAVFYQIYPRSFADGNGDGIGDFKGITEKLDYLVSL
                     GVDALWLSPHFPSPNWDCGYDISDYTNVAPEYGTLEDFKTFLKESHKRNIRVILDLVL
                     NHTSDEHPWFLESKSSRDNPKADWYVWVDTPPNNWQSCFDGDAWTYVPERKQYYYHYF
                     MKQQPDLNWHNPEVKAAMWDAVRFWLDLGVDGYRLDAIGTIYEDANLTPHTVPMTLAE
                     LRRFSELAETEEDKKLREKYWVEMFKNQWGQPGVHELMKELRSILNEYDGDRMLVGED
                     DNIDFMGDGEDELHLVFNFPLANVNRITPEHIRKNQTERIARLNQLAKGGWACNTLGN
                     HDRSRIYTFYGDKLHDAELARLNAALTLTLRGTPFLYNGEEIGMTDYLLPHISQVKDT
                     MGSWYYHTIVTEMGVDPEEAMFRTAEMTRDKNRTPMHWSNKANAGFSPEGVETWLPIN
                     PNYKEGINVFEQEQNPSSILNYYKHLLKVRKASPALVEGDYLPLHEDATDYFAFIRKS
                     DKQTVLVVLNYSDKRLRLNFSSDQDVNGKNLHTLFTSAVRSPADHKADNLTIAPFEVF
                     IAEVTA"
     gene            complement(4787..5053)
                     /locus_tag="PLR93_01360"
     CDS             complement(4787..5053)
                     /locus_tag="PLR93_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNH25631.1"
                     /translation="MIPDRVITLAELKRNTGERGTRMWIAHLGIVYDVTECPKWRAGM
                     HEFLHFPGQDLSSEMEDAPHEDEVFKHDCVKIIGKLEEQDVSTS"
     gene            complement(5050..5781)
                     /locus_tag="PLR93_01365"
     CDS             complement(5050..5781)
                     /locus_tag="PLR93_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25632.1"
                     /translation="MTNQTPIINFSPKKILIFFFSLVALLVALSIWGQHMRFFGVGDI
                     RGPIHEMFIDIMMTSFYLDYESNVPTFINALMLFIPALLLLAIGLWKSNIKDKYRFQW
                     NALAFIFFLLSIDEIASFHERLIKPMRAAVGSHGIFFFAWIIPGMAAIALFGFAFLTF
                     FLHLDKKFKLLFFFSLAVYISGVIGGEMVSGYFAANLGQKNFTYAVVASLEESVEMFG
                     CGLIIYSLLEYIKHYLPEGLTFKGS"
     gene            complement(5832..6869)
                     /locus_tag="PLR93_01370"
     CDS             complement(5832..6869)
                     /locus_tag="PLR93_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNH25633.1"
                     /translation="MKAVQFTGVNQPLQMQEIPIPEIGERDILVKIKAAGVCHSDAHY
                     RAGISQVRPVPLTLGHEVAGVVEKIGANVTNVKIGDRVALHYNLSCGDCYYCSTGNDQ
                     FCEKVLMLGHYTNGGYAEYIAVPARNAVHLPEEIPFEQGATLMCASATAFHALRKSRI
                     QGGETVAIFGAGGLGQSAIQLAKAFGALEVYAVDINESKLDLAKQHGAIPVNAQKVDA
                     VAEIKRLTYNKGVNVAIEMIGLPQTMKQALQVAGVMGRVVIVGVSNKMLEVQTYTELL
                     GNEVELIGSNDHHLQELPLLVDLARRNVLDTSKVVSKTIPLDADAINAALDELERFGS
                     DVRTVIVPASS"
     gene            complement(6873..7337)
                     /locus_tag="PLR93_01375"
     CDS             complement(6873..7337)
                     /locus_tag="PLR93_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNH25634.1"
                     /translation="MIQLAPIATVKNARIEIEDDNWGNVVSVIELDTSIPEEALLEIE
                     SFSHAEVIYYFHLVPDAKIETGSRHPRNNTAWPKVGILSQRGKNRPNRLGATIVKIVK
                     REGHQLHVQGLDAIDGTPVLDIKPVMREFLPREEIRQPEWATELMKNYWAKE"
     gene            complement(7339..>7762)
                     /locus_tag="PLR93_01380"
     CDS             complement(7339..>7762)
                     /locus_tag="PLR93_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25635.1"
                     /translation="QGKVDLTIGLEGDNWQARSSKTTPDGSANIETQITLMNSRVIQL
                     LTQDKNLWQWAGDQLYVDLDLSEENLPAGTRLSIGTAVIEVTATPHTGCKKFSARFGL
                     DALKFISASENKSLRLRGINARIIQPGEFKTGDLVQKI"
BASE COUNT         1989 a         1735 c         1843 g         2195 t
ORIGIN      
        1 ttgttgtcat aagtcgcctt aataaagtat tgtgataatt ttcacaaata tagtttacat
       61 gaccatgtca aatcagtcaa tgaaggatgt catatgatga atatgtaaat tatctgtcaa
      121 ttaaatcgat tacatttgta gttttctgct attttccgcc cttaatgcca tttggatctc
      181 agttagagtg ggtgcctcca gctcgaaaat cgtgctaccc aaaaattcag aaagccgatg
      241 gacatcacct gattgaataa ccggcagccg tttcgatgaa gggaattttt gatacaacat
      301 ctctcgggta atgtggcggg aaatttccaa agcagggaag cgccaccaat ctgcgagaaa
      361 gccgagtgta gggaacaagc caaaggaggg tcgatctaca tgagctggga tgacaatgcc
      421 attcaacttt atgacgagtt caatgatttc ttcaatggag aattttgtgg ctgtgagtaa
      481 gagccgcggc tctgtgcgga tgtattcacc ttgctcatcc acaacaaatt gttcaccaag
      541 atgctcggct ggattcaatt catcggggag ggcaaggtca acttggtgct gccaagcttc
      601 gaggtccgcg agagaggcga agaggcaaag caagtggaca tcttcacgcg attggatttc
      661 catgccgggc aatacggtca gcccagttcc ttccgctgcc ttttggacag atcccacatt
      721 cgcgctggcg ttgtgatcgg tgatggcgat aatatcgatg tgctgttcaa gcgcctcttg
      781 cacgatgagc ggaggaatca tttccacctc agcgcaggga gagaggacgg tatgaacgtg
      841 cagatctgca cgaaatgatt tcatacttaa cgcaagttca aatcccagag cgcgccgcag
      901 acttcataac ttgcttgtgg tgtggatagt aagatgactc cctgttcatt ggctttggtg
      961 atggtggctt catcaggctg agcgttttcg gtgatgatga cggctgagac atcaagcatt
     1021 gccgcaacag cgacgatgtt catatgtgcc tgtaatgtga cccagatgtt gccattcttt
     1081 gcaccagcca taacgcatga aagcaagtcg gacgtgtagc cacccttggg agtaatggat
     1141 gaaaaatcac gcggttcggt aaggatattg agttgaagtt tttcgatgat agtttgaaga
     1201 ttcatagtta tccctttttg ctgtttaggc tttcagttgg gacgttaatg cgcatggtga
     1261 gctttgtgcc tttaccagtt gttgattcaa gctccattga gtctacacag cgttttatgt
     1321 tgaccagtcc catacctgca ccaaagccca tttcgcgaac tttatctgtc gccgtggagt
     1381 aacctgcttg ttgtgctttt tgcacatcgg gaatgccagg accatcatca gtagtgatta
     1441 ggctaatgcg atgaggttcc acttcaagtt tgagaacgcc gccattggtg gtgtggataa
     1501 ttagattcat ctctgcttca taagcagcga ttccgcagcg ccgtgccagt tgtgcatcag
     1561 cgccgaggcg aagcagggca cgtttgatat tgctggaggc attaccgcca tgcataaaat
     1621 ctccaacttt gatgttgtat cgtaaaacaa gggtagtgcg gtctgaaaca atatcgtcga
     1681 ataaatggct ggcgcgatat cgccgaactt cttcttcctt gtaatcacgt tctactgcca
     1741 atagaatacc gcgtgtgata tcccctttgg tgatgatgcc ctctaacttt tggtgttcat
     1801 ccaccaccgg caaacgcccc agccgttttt cgctgaaggt ttttatcgct tcaacgacac
     1861 tgtctgtagt tttgacggtg acaagggttg tgctcatgta ggctttaacc ggaagtgata
     1921 acccgtttgt ttgtagagct cgaacaatat cttccagact gatcacacca atcaaggtgc
     1981 catcgctgac gaccggcaag ccagagatac ggttgactct caaaagctct aatgcgctgg
     2041 aaagcggcac atcagatgtg gttgtgatga cgttcttgat catcacttcg cgcactttca
     2101 ggtcatatga aagttcctcg atgcgattga tctcgtgtgc atcgcggtct gtgagcggac
     2161 gatgtcgccc aaacttttct tgttcagtcg gttgcctagt cggatccatt gccttcgtag
     2221 ctttttaatc ctgagtcatg aagttttccg catagctcaa acatacccat tgggcttgtg
     2281 atcaacggaa tgttttcctg atttgcaata tcgatggtct cttgctgagg tctcttgccg
     2341 cggataaaaa cgatcgcgcg gaaatctgcc atttgcgcgg ttcggactac ctgtgggttg
     2401 gtcagccccg tcagcaatac ggctcgcgga cggctgtacg ccagtacatc gctcattaag
     2461 tctgcacagc aaccgccatg aatgtttaca tcagtattca gctcgggggt tagtaattca
     2521 ccttctgcaa tcacaataat gtctttcaaa ttcatattca tatataaagg gatggtcctt
     2581 tcacctcata ataaaactct ctttaaaatt attccctact tagtctgttt aggtaagttt
     2641 cttttctcat gctcggtagg tgactattga cctgttaagt gataaatttc acaaatatat
     2701 aacaaataaa aaaacgctct ttagagcgtt tttttatttg ttacttttct tcagtaggtc
     2761 tttcagaagc gtattggctc cataaagtag ctagatgttc gatttctttt ttcatgcgag
     2821 ctgcagtttt atctgagttg cggtgaactt tacagaaaat atctgggtct gcgagaaagg
     2881 caccaaggca agcctcggtg caaagcaaaa ttttcttgcc tttgtattca gcaaaaggaa
     2941 aatactgtgg gtcactatct cggaagatac gtccgcacag ggtggcgaat gcctttggtt
     3001 tcgcccgttc ttcactcatg cagtgacttc cgcaatgaag acttcaaatg gcgcaatggt
     3061 gaggttatct gctttatggt cagcagggga gcggacagca ctggtgaaca aggtatgtaa
     3121 gttctttcca ttcacatctt gatcacttga gaaattcaag cgtaagcgtt tatcggaata
     3181 attcaataca accaataccg tctgtttgtc tgactttcta atgaatgcaa agtagtccgt
     3241 tgcatcttca tgaagcggaa gataatctcc ttctaccaat gcaggtgacg ccttacggac
     3301 cttgagcaag tgcttgtagt aattcaagat cgagcttggg ttttgctcct gctcaaaaac
     3361 attgatgccc tctttataat ttgggttgat cggcagccaa gtttctacac cttctggaga
     3421 aaaaccagca tttgctttat ttgaccaatg catgggggtg cggttcttgt cgcgggtcat
     3481 ttctgctgtg cggaacatgg cttcttctgg gtccacgccc atttcggtga cgatggtatg
     3541 gtaataccag gagcccattg tgtctttaac ctgcgagata tgcggcagaa gatagtcggt
     3601 catgccgatt tcttcaccat tatagaggaa aggggtgccg cgtaaagtaa gggtaagggc
     3661 agcattaagg cgagctaatt cggcgtcgtg cagcttatcg ccataaaacg tgtaaatgcg
     3721 agaacggtcg tggttaccca atgtattgca agcccagcca cctttggcaa gttgatttag
     3781 gcgtgcaatc cgttcggttt gattttttcg aatgtgttcc ggagtaatgc ggtttacatt
     3841 tgccaagggg aagttaaaga caagatgcaa ctcatcttcg ccgtcgccca taaaatcaat
     3901 gttgtcatct tcgccaacca gcattcggtc gccgtcatat tcgttgagaa tgctacgcag
     3961 ttctttcatc aactcgtgaa cgccgggttg accccattga ttcttgaaca tctcgaccca
     4021 atatttttct ctaagcttct tatcttcttc ggtttctgca agctctgaga agcggcgcaa
     4081 ttcagcaaga gtcatgggaa cggtatgagg cgttaggttg gcatcttcat aaatggtgcc
     4141 gatggcgtca agacgatacc catccacacc aagatccagc caaaagcgga ccgcgtccca
     4201 catagcggct ttgacttctg gattgtgcca gttcaaatct ggctgttgtt tcataaagta
     4261 gtgatagtaa tactgttttc gttcgggaac ataggtccat gcatcgccat cgaagcagga
     4321 ttgccagttg ttgggcggag tatctaccca aacgtaccaa tccgctttgg ggttgtcgcg
     4381 gctggacttg gattccagga accaagggtg ttcgtcagaa gtgtgattca agacaaggtc
     4441 cagaatgacg cggatgtttc gcttgtgaga ctcttttagg aaggtcttga aatcttcgag
     4501 ggttccatat tctggggcaa cattggtgta atctgaaatg tcgtaaccgc aatcccaatt
     4561 gggggaggga aaatgcggcg aaagccaaag cgcatccacg ccgaggctga cgaggtaatc
     4621 aagtttttcg gtaataccct taaagtcacc gataccatct ccattgccat cagcaaacga
     4681 acgcggatag atttgataaa aaacagcagt ttgccaccat ttgagtgatg tcattgtaga
     4741 tgtaattcct tttgcattga gagtttgaaa gtttataggc aagaaattaa gaggttgaaa
     4801 cgtcctgctc ttccaacttc ccaattattt ttacgcagtc atgcttgaat acttcatctt
     4861 catggggtgc atcttccatt tcgctgctca gatcctgacc tggaaaatgc agaaactcat
     4921 gcattccggc tctccacttg gggcattcgg tcacatcgta gacgatgcca agatgggcga
     4981 tccacatgcg cgtcccacgt tcgcctgtat tgcgttttag ctctgcgaga gtgataacac
     5041 ggtctggaat catgagcctt tgaacgtgag cccttcggga agatagtgct tgatgtattc
     5101 aagcagggaa taaatgatga gtccacagcc gaacatttca acagattctt ctaaactggc
     5161 gaccacagcg taggtgaaat ttttctgccc gaggttggca gcaaaatatc cactaaccat
     5221 ttctccgccg attacgccgc taatatatac tgccagcgag aagaaaaaga gcaatttgaa
     5281 cttcttgtcg aggtgtaaga aaaaggtgag aaaagcaaag ccgaacagtg caatagcagc
     5341 catgccggga ataatccaag cgaaaaagaa aataccgtgc gaaccgactg cggcacgcat
     5401 cggtttgata aggcgttcat gaaatgaagc aatttcatct atcgaaagca agaaaaagat
     5461 gaaagcaaga gcattccatt gaaaacgata tttatctttg atgttgcttt tccacaagcc
     5521 aatggcaaga agtagaagtg ctggaatgaa aagcattagg gcattaatga aggtcggcac
     5581 attgctttca tagtccagat aaaaggatgt catcatgatg tcgatgaaca tttcgtgtat
     5641 gggaccgcga atatcgccaa cgccaaagaa gcgcatgtgc tgtccccaaa tgctgagtgc
     5701 cactaacaac gctacaagag agaaaaagaa aatcagaatt ttcttggggc tgaaattgat
     5761 gataggagtt tggtttgtca tagatacctg tgagcgttga ttatacgtcg aaacgctgga
     5821 cagttgtcgg cttaggaaga ggcgggaaca atgactgtgc gcacgtcact gccaaagcgt
     5881 tccaactcat cgagcgcggc gttaatggca tccgcgtcca gtggaatggt tttgctgact
     5941 acttttgatg tgtctaatac atttcgacgg gcgaggtcaa caagcaaggg aagttcttgc
     6001 aagtgatggt cgttggaccc gatgagttcc acttcattac caagcagttc tgtgtaggtt
     6061 tgcacttcca gcattttgtt cgaaaccccc acaatgacca cgcgtcccat tacccctgca
     6121 acttggagcg cctgtttcat ggtctgtgga agtccaatca tctcaatggc aacattgaca
     6181 cctttgttat aagtaaggcg tttgatctct gccactgcat ctactttttg ggcattgacg
     6241 ggaatcgcgc catgttgttt agcaaggtca agtttcgact cattgatatc gacggcatat
     6301 acttcgagag cgccaaacgc ttttgccaat tgaatagcag actgaccgag tccgcctgcg
     6361 ccgaagattg ctaccgtttc cccgccttga atgcggctct tgcggagtgc atgaaaggca
     6421 gtggcactgg cacacatcaa agttgcacct tgttcaaaag gaatttcttc tggtaagtga
     6481 acggcattgc gtgctggcac ggcaatatat tctgcgtaac cgccgttggt gtaatgtcca
     6541 agcatcaaaa ctttttcaca aaattgatcg ttgcctgttg aacagtaata gcaatcgcca
     6601 cacgaaaggt tgtagtgaag tgcaactcga tcaccaattt ttacattggt tacgtttgca
     6661 ccgatttttt caaccacgcc cgctacttca tgccctaagg taagaggtac agggcgcact
     6721 tgtgaaatgc cagcacgata atgagcatcc gaatgacaaa cccccgctgc tttgatcttt
     6781 actaaaatat cccgctcgcc aatttctgga attggaatct cttgcatttg taacggttgg
     6841 ttgacaccag taaattgaac tgctttcatg tattactcct ttgcccaata atttttcatt
     6901 aactctgtag cccattcagg ctgacgaatt tcttctcgcg gtagaaattc acgcatgaca
     6961 ggttttatat ccaagacagg agttccgtcg atggcgtcca atccctgaac atgtagttga
     7021 tgaccttctc gcttcactat tttaacgata gttgctccaa gcctattggg cctgttctta
     7081 ccccgttggc tgagaatccc tactttgggc caagccgtgt tgttgcgcgg atgacgtgac
     7141 ccggtttcga tttttgcatc tggaaccaaa tgaaaatagt aaatgacttc tgcgtgtgaa
     7201 aaactttcga tttccagcaa cgcttcttcg ggtatagatg tgtccaattc aatgacagag
     7261 acgacattac cccagttatc atcttcgatt tcaatgcgcg cattttttac agtagcaata
     7321 ggagctaatt ggatcatatt agattttttg taccaagtca ccagttttga actcaccggg
     7381 ttgaattatg cgagcattga ttccgcgcaa gcgcagcgat ttgttttcgg aggcactgat
     7441 aaattttaaa gcatccagcc cgaagcgagc agagaatttc ttgcaacctg tgtggggcgt
     7501 agccgtcact tctatgactg ctgtgcctat tgaaagccga gtccctgcag gtaggttttc
     7561 ttcactaagg tcaagatcca cataaagctg gtcacctgcc cattgccaaa gatttttatc
     7621 ttgggtcaac aattggatca cgcgtgaatt cattagagta atttgtgtct ctatgttggc
     7681 agacccatcc ggcgtagttt tgctgctgcg ggcttgccaa ttatcgcctt ccaagccaat
     7741 ggttaagtcc actttgcctt ga
//
LOCUS       DAOQFT010000029         7750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4521_length_7750_cov_7.906953, whole genome shotgun sequence.
ACCESSION   DAOQFT010000029 DAOQFT010000000
VERSION     DAOQFT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4521_length_7750_cov_7.906953"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PLR93_01385"
     CDS             <1..1274
                     /locus_tag="PLR93_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNH25636.1"
                     /translation="IQALRKKIQLTWAGAFVALGAFILFVFFSAALGSLINPISLRGQ
                     VYNGRTIRALITLFIGIAFFVSAVWMNKNEDDLRFTVQWIFAGLCLDIAWSGLQAVTF
                     YTGLLEKEMVTHWQLAFSMRELVRTNRISGLAYEPAWLAGQIATTFFPFLFAAVLTKY
                     RVTRLSWLEPALLAVTTLVLLATYSRGGLLIILGTSGLLMLLFGRDILKGMWAWFISG
                     FRGRISDLALRIGMVAVLVGIVAGAVLFLGQKNYFRRLWEINAESLSEYIVDINAGAR
                     GAYSVGALGAFSEYPLTGVGLGGSGFYIYKNLPDWALTTVPEIAKQLNPSNKLYPNPK
                     NLYVRLLAETGLFGFVLFFAFLFNVLGDMLSLLKRSEPWARFAAVAGVFAWVAIALYN
                     FTQDSLTTPNIWIVPGILVGLATHQLNKEVS"
     gene            1271..1999
                     /locus_tag="PLR93_01390"
     CDS             1271..1999
                     /locus_tag="PLR93_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNH25637.1"
                     /translation="MIVLSVGMPRAGSGWHYNLIHDLMKTTGCADAQDIRKKYGLQKI
                     LTEVNCNIGVLSPRRLAMVTVPAVLGNTFVIKAHASPSSASRFLASLGMLRITYIYRD
                     PRDAMLSAFDYGQRALAKGRPNAFSHLSDFEKSVDFMMEYVHIWEKWMKEKNVLIARY
                     EDLLTNYEVESAKLVDYLKLDANKPEVRAVIEQYRPGANDGQQGLHFYKGKIGRFRDA
                     YTSEQQNVLLEKLSPYLERMNYEK"
     gene            2078..3835
                     /locus_tag="PLR93_01395"
     CDS             2078..3835
                     /locus_tag="PLR93_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNH25638.1"
                     /translation="MKRFETPYMADWFAISLRWIMLVGLIVSLGLGQRLDASASWPLG
                     LMIVWNLGMTALAGLNIRTQYHRRLNIAMDLILTAAFFWAQGGVRGPAFWAGLLPILT
                     GSIYFELIGAVVAAILFSGFVIYTGTRVDGNITLAFLVAAILSLLGVLFGFLGRRLTD
                     HMRKNRALWVDAEEKKRAIQNERMRAIYELTSTLSTTLSYKRVLDSALDMSASALNPN
                     LDPEQTTSDPLVGAVMLFNAGKLRIGSARRFTNADMRITFDGAEGVLKKALDEGEPVT
                     FKDIGYDQELGRVIALRNCTGGYVFPLRSGFNVYGVLLFAHPEPDFFNTERTNLLDII
                     GRQAVIAIQNARLYQDLVEEKERLADVYEEARKKLARDLHDGPTQSVAAMAMRLNIAR
                     RMLTKDTKMANEELVKLEELAHRTTKEIRHMLFTLRPLILESQGLGAAIQSMADKMME
                     TFSQRVVVEIDDRAVQQLEMGKQGVIFYIIEEAVNNARKHAAAEAITVRLRQVDTGIV
                     LLEIQDNGAGFDVKAITQNYDKRSSSSLGMINLQERAELVNGLLNIDSAPGKGTNVQV
                     YIPLTEEAADRLHHARRSA"
     gene            3839..4615
                     /locus_tag="PLR93_01400"
     CDS             3839..4615
                     /locus_tag="PLR93_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH25639.1"
                     /translation="MPFVQGIHYSFQQSKETNPSRPPLIFIHGAGGSFLSFHPYLRHM
                     QGETIYALDLSGHGKSVGKGRQSIEEYADDIARFMDDLQIASAVMVGLSMGSGIALSF
                     ALKYPHKVSALVVMGGGAKLRVAQSTLENVGKPETFEATVETINRACFSTYASNELVE
                     LSKKSMLSTGPDILFSDYLACNHFDVTTQLNQIRIPTLLLCGMEDIMTPPKCSQYLRD
                     HLPNAQLHLLEKTGHMVTLEQPELVAKLLKQFLDELPPLS"
     gene            complement(4698..5294)
                     /gene="rdgB"
                     /locus_tag="PLR93_01405"
     CDS             complement(4698..5294)
                     /gene="rdgB"
                     /locus_tag="PLR93_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNH25640.1"
                     /translation="MSKLLIATNNQGKVRELQELLTGSGIEFVTPAQINLQIDVEEDG
                     STYQENAGKKAIAFAKASGLISLADDSGLEVEALGGAPGLYSARYSPKPGANDKDRRD
                     FLLSNLQDKPRPWKAHFHATIAVANPTGEVEFTDGNCYGEIIPEERGSGGFGYDPIFF
                     MPELGKTMSELDMSEKNRLSHRARAVIAAMPILKRIFA"
     gene            complement(5291..6337)
                     /gene="queG"
                     /locus_tag="PLR93_01410"
     CDS             complement(5291..6337)
                     /gene="queG"
                     /locus_tag="PLR93_01410"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNH25641.1"
                     /translation="MFNNSITLKQAIKEKARQLGFFLAGVTSSASLAHFDIFQEWLDA
                     NHHASMDYLASDRSRLRRADPKQILSECKSVLVLALPYSPISKNSSEFQIASYALGDD
                     YHEVIPPRLQEIVQFIEQQVGHPIPNRYYTDTGPVLERELAQRAGLGWIGKNSMLIHP
                     QKGSTFLLAEILLGIELEPDEPFQTDHCGTCTRCMDACPTQCILPNRTIDSNRCISYL
                     TIENKDDIPEALRPQIQNWVFGCDICQQVCPWNRFSLPADPDLKPSIPLPVLTSDLLL
                     TSSEFNQRFKRSPIKRTKRRGYLRNLAVAVGNQKNKKDLPLLHQATQDEETLVQSHAQ
                     WAIETINRNNTGNL"
     gene            complement(6327..6953)
                     /locus_tag="PLR93_01415"
     CDS             complement(6327..6953)
                     /locus_tag="PLR93_01415"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNH25642.1"
                     /translation="MKKIILPLIIVVLLSACAPATPIEPTPDVNAVRTSAASTVIAEF
                     TLTAAFFTPTPLPLPTETPTPFPTETPAVLPSIDPTLAALTPDALCDDYDFDPATIDV
                     TIPDGTPMTPGQDFVKTWKIKNTGICTWDTSYKAIFSYSNPPNQQMSAQPIPLAALVA
                     PGQEVDVSVQFTAPTTPGEYLGVWQMVNAKGIPFGQPGKQLTVKIVVQ"
     gene            complement(6955..7689)
                     /locus_tag="PLR93_01420"
     CDS             complement(6955..7689)
                     /locus_tag="PLR93_01420"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNH25643.1"
                     /translation="MNFRKFRWLMISTLAAVLVSSCNFGATPPPTQDTGAIQTQAFGQ
                     VLTQVASQATQTAAAIPPTPLPTNTPVPTNTLPPIPTSDAFNIPTNTPFVFNTQQPGL
                     TPQSGLSFTATVGVYSTTTTKNGCNDGYLVSESQPYDGKTMSANIDFDKTWDFINTGT
                     CTWDEGYTFAFVPEFSTAPLYTVPKDYIIPKNGVFTKPGETRSFKVSLRVPKDPGEYI
                     WYYKIKDDGGQYFGSLVWVKVISVRE"
BASE COUNT         1877 a         1699 c         1969 g         2205 t
ORIGIN      
        1 taattcaagc cttgcgtaaa aagattcaac ttacatgggc aggcgcattt gttgcactgg
       61 gcgcttttat tctttttgtg tttttttctg cggcgcttgg ttcactcatc aaccccattt
      121 cgctgcgcgg acaagtctat aacggtcgca ccattcgtgc cttgatcaca ctctttattg
      181 gcatcgcctt ctttgtcagt gccgtgtgga tgaataaaaa cgaagacgac ctgcgcttca
      241 ccgtccaatg gatttttgca ggtctatgtc ttgatatcgc ttggagcggt ttgcaagcgg
      301 ttacctttta taccggcttg cttgaaaaag aaatggtcac ccattggcag cttgcttttt
      361 cgatgcgcga acttgtccgc acgaatcgca tctctgggct tgcctatgaa ccggcgtggc
      421 ttgccggtca aattgcgacg acattcttcc ccttcctctt tgcggctgtt ttgacgaagt
      481 accgcgtcac acgcctatcg tggctggagc ctgcgttgct agcagttaca acattggtgc
      541 ttttggctac ctattcccgc ggcggcttgc tcatcatcct tggcacatcg ggcttgctta
      601 tgttgctctt tggtcgcgat attcttaaag gtatgtgggc ttggttcatc agcggctttc
      661 gtggacgtat ctctgacctt gcattgcgta ttggtatggt tgcggtgttg gtcggtattg
      721 tggctggtgc ggtcttgttc ctcgggcaga agaattactt ccgccgcttg tgggagatca
      781 acgccgagag tttgagcgaa tacattgtgg atattaacgc cggggcgcgc ggagcgtact
      841 cggttggggc gttgggagca ttttcagaat acccgttaac aggcgtcggc ttgggaggaa
      901 gcgggtttta catctataaa aatttgcccg attgggcgtt gaccaccgtc ccagaaatcg
      961 cgaagcagtt gaatccatct aataaactgt acccgaaccc caagaattta tatgtacgtt
     1021 tgcttgcgga aactgggttg tttggcttcg ttctcttttt tgcatttctc ttcaatgtgc
     1081 ttggagatat gctctccctg ctcaaacgga gcgagccatg ggcgcgcttt gcggcggtgg
     1141 ctggtgtatt tgcgtgggtg gctatcgccc tgtataattt cacccaggat tcgcttacca
     1201 ccccgaatat ctggatcgtg ccggggatct tggtggggct tgctacacat caattgaaca
     1261 aggaagtttc atgatcgttc tttctgttgg gatgccgcgc gctgggtcgg gctggcacta
     1321 taacctcatt cacgacctga tgaaaaccac gggctgtgcc gatgcgcagg atatccgtaa
     1381 gaagtatggg ttgcaaaaga tcctcacaga ggtaaattgc aatattggtg tgctttctcc
     1441 gcgccgtttg gcgatggtga ctgtgccagc cgtactgggc aatacctttg tgatcaaggc
     1501 gcatgcgagt ccttcatcgg cttcccgctt cttggcttca ctgggtatgc tgcgtattac
     1561 ctacatctac cgcgatccac gtgatgccat gctctctgcc ttcgactacg gtcagcgtgc
     1621 tcttgccaag ggacgtccca acgctttttc acacctttcc gattttgaga aaagcgtgga
     1681 ttttatgatg gaatatgtcc acatttggga aaagtggatg aaagagaaaa atgtgttgat
     1741 cgcgcgttac gaagacctac ttaccaacta tgaagttgaa tctgctaaac tggtggatta
     1801 cctcaagttg gatgcgaaca agcctgaggt ccgtgcggtg atcgaacaat atcgccctgg
     1861 cgcaaatgat gggcagcaag gcttgcattt ttacaagggc aagatcgggc gtttccgtga
     1921 tgcgtataca agcgagcagc aaaacgtgct tttagagaaa ttaagcccgt atttggagcg
     1981 tatgaactac gaaaagtaat atttgcacta ggtgtaatct gactgtaacg tcggggatgt
     2041 tgtcgaaaca tccctttttc gttatcatag ggcagcgatg aaacgatttg aaactcccta
     2101 catggcagac tggtttgcca tctcactgcg ctggatcatg ctagtgggtc ttatcgtttc
     2161 tcttgggctt ggacagagat tagatgcgag tgccagctgg cctctggggc tcatgattgt
     2221 ttggaacttg gggatgacgg ctctagctgg cttaaacatc cgtacacaat atcatcgccg
     2281 cctcaatatt gcgatggatt tgatccttac agcggcattc ttctgggcgc aaggtggcgt
     2341 tcggggacct gctttttggg caggattgct ccctattttg accggttcca tctactttga
     2401 gctaattggc gcagtggtgg ctgccatttt gttctcaggc tttgttatct acaccggcac
     2461 ccgggtggat gggaatatta cccttgcatt tctcgtagct gcgattctga gccttttagg
     2521 agtactcttt ggttttctag gtcgccggct aactgaccat atgcgcaaga atcgagcttt
     2581 gtgggttgat gccgaagaaa agaaacgtgc cattcaaaat gaacgtatgc gtgcgatata
     2641 cgaattgact tccacgcttt ccacgaccct tagctataaa cgtgtgcttg actcggcgct
     2701 cgatatgagc gcttctgcat tgaatcccaa tcttgaccca gaacaaacga ccagcgaccc
     2761 attagtgggc gctgttatgc tttttaatgc gggtaaatta cgcattggct ctgctcgtcg
     2821 tttcaccaac gctgatatgc gcattacttt tgatggtgca gagggcgttt tgaaaaaagc
     2881 gctcgacgaa ggggagcctg tcactttcaa agatattggt tacgatcaag aactgggacg
     2941 tgtcattgcc ttgcgaaatt gcacaggcgg ttatgttttc cctttacgaa gcggtttcaa
     3001 tgtatatggt gtgcttttgt ttgctcaccc tgaacctgat ttttttaata ccgaacggac
     3061 taatcttttg gatatcattg ggcgtcaggc agtgattgcc attcagaatg cccgccttta
     3121 tcaggacctg gtggaagaaa aagagcgcct cgcggatgtg tacgaagagg ctcgtaaaaa
     3181 gctagcaaga gacctgcacg atggacctac ccagtctgta gctgccatgg caatgcgttt
     3241 gaacatcgct cgccgtatgt taacgaaaga caccaaaatg gcgaacgaag aactcgtcaa
     3301 attggaagaa cttgcacatc gaaccaccaa agaaattcgc catatgctct ttaccctgcg
     3361 cccgttgatt cttgagtcgc aaggtttggg ggctgccatt caatccatgg cagataaaat
     3421 gatggagact ttctcacaac gtgtggtggt tgagatcgat gaccgtgctg ttcagcaatt
     3481 ggagatgggc aaacaaggcg tcattttcta tatcatcgaa gaagctgtta acaatgctcg
     3541 caaacatgct gctgccgaag cgattaccgt ccgcttgcgc caagtggata cagggattgt
     3601 gcttttagaa attcaagaca acggcgcagg ctttgacgtg aaagccatta cgcaaaatta
     3661 tgacaagcgt tccagcagca gcttgggtat gatcaacctg caagagcgtg ctgaattggt
     3721 gaacggcttg ctaaacatag actccgcgcc gggcaagggc accaacgtgc aggtttatat
     3781 tccgcttacc gaagaagctg ccgatcgctt gcaccatgct cgcaggtctg cataagccat
     3841 gccgttcgtg caaggcattc actactcgtt tcaacagtcc aaagaaacaa atcccagccg
     3901 cccaccgttg atttttatac acggtgcggg cggcagtttt ttatcctttc acccatatct
     3961 acggcatatg caaggagaga ctatctatgc gttagacttg tcagggcatg gcaagtctgt
     4021 gggcaaaggt agacagtcta ttgaagagta tgctgatgat attgcgcgtt ttatggatga
     4081 ccttcagatt gcatcagcag tcatggttgg gctttcaatg ggaagtggca ttgctttatc
     4141 ttttgcactg aaatatccgc ataaagtaag tgcattggtt gtgatgggtg gcggagcaaa
     4201 attaagagtt gcacaatcca cgcttgagaa tgtgggtaag cctgaaacct ttgaagccac
     4261 tgttgaaacc attaaccgtg cgtgttttag tacatatgct tctaatgaac ttgttgaact
     4321 ttcaaaaaag agtatgctga gcactggtcc agatatttta tttagcgatt atctcgcttg
     4381 taatcatttt gatgtcacta cccagttgaa ccaaatcaga atcccaacac tgcttctctg
     4441 tggcatggaa gatatcatga cccctccaaa atgttcacaa tacttaagag accatcttcc
     4501 taatgcccaa ctccacttgt tggagaaaac tggacatatg gtgacgcttg aacagccgga
     4561 gttagttgca aagttattga aacagttttt ggatgagctc ccgcctcttt cctaacgggt
     4621 agctgattct gcccatttag ttttttctta atctaaaaat agccagtcac ttgtggtgtg
     4681 actggctatt tattttttta tgcaaaaatt cttttcaaga ttggcattgc cgcaatcact
     4741 gccctcgccc gatgactgag cctattcttc tcactcatgt ccagttcaga cattgtcttg
     4801 ccgagttccg gcatgaaaaa gatagggtca tatccaaacc cgccactacc acgctcttca
     4861 gggatgattt ctccatagca atttccatca gtaaattcaa cttcaccagt agggttggca
     4921 accgcaatag tggcatgaaa gtgggctttc catgggcgtg gcttgtcttg gagattgctc
     4981 aaaagaaaat cccgccggtc tttatcattt gcaccaggtt tcggggaata ccgtgcggaa
     5041 tataagccag gcgcgccacc taatgcttcc acttccagcc ctgagtcatc tgcgagggag
     5101 atcaagccgc tggctttggc aaatgcgatg gctttcttgc cggcattttc ttgataggtg
     5161 gaaccgtctt cctcaacatc gatttgaagg ttaatctgtg caggagtcac gaattcaata
     5221 cccgaacccg ttaataattc ttgaagttcg cgtactttcc cttggttatt ggtggcaata
     5281 agaagtttac tcataaattt cctgtgttat ttctatttat agtttcaatt gcccattggg
     5341 catgagattg cacgagagtt tcttcatctt gagtagcttg atgcaagaga ggtaagtctt
     5401 ttttattttt ttgatttcca acagcaacag caaggtttcg caaatatcca cgtcgcttgg
     5461 tgcgtttgat tggagaccgt ttgaagcgtt gattaaactc ggaggatgtc agaagcaggt
     5521 cagaggtcaa gacaggaagc ggaatggatg gtttgaggtc agggtctgct ggcagggaga
     5581 atcgattcca cgggcagact tgctgacaga tgtcgcatcc aaaaacccaa ttttgtattt
     5641 gaggtcgcaa ggcttcagga atatcatctt tgttttcgat ggtgagataa gagatacatc
     5701 gatttgaatc aatcgtgcga tttggtaaga tacattgggt tgggcaggca tccatgcaac
     5761 gggtgcatgt gccacaatga tcagtttgaa aaggctcatc cggttcgagt tcgataccaa
     5821 gcaagatttc tgcaagtaga aaagtagaac ctttctgcgg atgaatgagc atggaattct
     5881 tcccgatcca cccgagcccg gctcgctgcg ccaattcgcg ttctaagacg ggacccgtat
     5941 ctgtgtaata gcgatttgga ataggatgcc caacttgttg ctcaataaat tgtacgatct
     6001 cttgcaaacg tggtggaatg acttcgtggt agtcatctcc aagtgcatat gatgcgattt
     6061 gaaattcaga actatttttt gagattgggg agtatggcag tgcaagaact aaaacagatt
     6121 tacattcgga caaaatttgt tttgggtctg cgcggcgtaa ccgactgcgg tctgaggcga
     6181 ggtaatccat gctggcatga tgattcgcgt ccagccattc ttgaaaaata tcaaaatgtg
     6241 caagtgatgc agaagaagta acgccagcta ggaaaaatcc cagctggcgc gctttttctt
     6301 ttatagcttg ctttagtgtg attgaattat tgaacaacga tctttacagt gagttgcttg
     6361 cccggctgtc caaatggaat gcctttcgca ttcaccattt gccagacgcc caagtattca
     6421 cctggggtgg taggcgctgt aaactgaaca gatacatcta cttcttgacc aggagcaacc
     6481 agtgccgcaa gcggaatggg ctgagcgctc atctgttgat tgggcgggtt gctatatgag
     6541 aagatcgctt tgtaacttgt atcccaagta cagatgcctg tatttttgat cttccatgtt
     6601 ttcacaaaat cttgcccggg tgtcataggc gtcccatcgg gaatggtcac atcgatggtg
     6661 gctgggtcaa aatcataatc atcgcagaga gcatctgggg tcaacgccgc caatgttggg
     6721 tcgatcgaag gaagaaccgc cggagtttct gttgggaagg gcgttggggt ttcagtgggc
     6781 agcgggagtg gtgtaggcgt gaagaaagct gcggtcaatg tgaattccgc gatcactgtg
     6841 ctggctgcgg atgtgcgaac tgcgtttacg tcgggcgttg gctcgatagg tgtggcaggt
     6901 gcacaggcag aaagcaaaac tactatgata agaggcaaaa taatcttttt cattttattc
     6961 ccttacagaa atgaccttta cccatactaa tgaaccaaaa tactgaccac catcatcttt
     7021 gatcttgtaa taccagatat attcgcctgg gtctttaggg actcttaaag aaactttgaa
     7081 tgatctagtt tcgcctggtt tagtgaaaac accattttta ggaattatat aatcctttgg
     7141 aacggtgtag agtggcgcag ttgagaattc tggcacaaat gcaaaagtat agccttcatc
     7201 ccaagtacat gtacctgtat tgatgaaatc ccaagtttta tcaaagtcaa tattcgcact
     7261 catggtttta ccatcataag gttgagactc gcttacaaga tatccatcat tgcatccatt
     7321 ctttgtggtc gtggtgctgt aaacacctac agttgcagtg aaggatagtc cactttgtgg
     7381 ggttagcccc ggttgttgcg tattgaacac gaacggcgtg tttgttggaa tattaaaagc
     7441 atctgatgta gggatcggag gtagggtatt tgtagggaca ggggtattgg ttgggagtgg
     7501 ggtaggcgga atagcagcag cggtctgcgt tgcttgactg gcaacttggg tcagaacctg
     7561 accaaatgcc tgagtttgaa tggcgccggt atcctgtgta gggggagggg tagctccaaa
     7621 attacaggag ctgacgagga cagccgcaag agttgaaatc atcagccagc gaaacttgcg
     7681 aaaattcatg gtagaacctc caaaaatggt taactttatt ataacgcaac catgtgagtg
     7741 ttttttaagt
//
LOCUS       DAOQFT010000030         7750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4522_length_7750_cov_7.143860, whole genome shotgun sequence.
ACCESSION   DAOQFT010000030 DAOQFT010000000
VERSION     DAOQFT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4522_length_7750_cov_7.143860"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2062)
                     /locus_tag="PLR93_01425"
     CDS             complement(<1..2062)
                     /locus_tag="PLR93_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNH25644.1"
                     /translation="MATDIKSIISQMTLEEKAALCTGASNWSTTPVERLGLPEMIVSD
                     GPHGVRRVADVHSMGQKSLPATCFPTASCTASTWDVDLIKQMGIALAEECIALNVDVL
                     LGPGVNMKRSPLNGRNFEYFSEDPFLAGELAANFINGVQSKGVGTSLKHYAANNQEFQ
                     RFSISAEIDERTLREIYLPAFEKAVKQAQPWTVMCSYNKVNGTFASEHHQLLTKILKD
                     EWGFEGLVVSDWGAVRNRVAALKAGLDWEMPGPQDLRVKAVVEAVRSGELNEATLDES
                     VRRILRIVFKSKETPKGNGSFDVDAHHELARKIATEGVVLLKNDGLLPLKDHQHIAVI
                     GRSAESAHFQGGGSSHINPTKVAMPYKELQSRAENAELTYAEGYPTDNSFQQGMIDDA
                     VGLAEAADVAILYIALPTFKESEGYDRKDLDLTVQQIALIKAVSKVQPNTVVVLNNGA
                     PVAMSEWIDGVPAVLEGWMMGQAGGAALADILFGRVNPSGKLAETFPLKLTDTPSHIN
                     WPGGAGKVHYGEGIFIGYRYYDTKEIPVLFPFGHGLSYTTFEYSNAKVSSKNFKDVDG
                     LTVTVDVKNTGKLAGKETVQVYVHDQKSDLMRPVKELKGFAKVDLQPGETKTVSIHLD
                     FRAFAYYHPEHKQWITEDGDFDILIGSSSADIRSHLTTTLQSTLDLPCVLDIESTMRE
                     WMADR"
     gene            complement(2076..4535)
                     /locus_tag="PLR93_01430"
     CDS             complement(2076..4535)
                     /locus_tag="PLR93_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNH25645.1"
                     /translation="MQKQSLTSLWQFRQLGSIEWLPAQVPGGVHTDLMALARIPDPFV
                     GDNEKRVAWVAESDWEYHTTFNVQADVFQRKNIWLVCDGLDTLALLTLNGKALGETNN
                     MFRQYRFDIKSLLKAEGNELTITFKSPVKFVTEKNAARPMNGVSQAIAGGPHLRKAPC
                     HFGWDWGPQLPPIGIWKDIYLEAYDSARISEVHLRQKHDNGKVTISARVEVENGNAPL
                     SATLLVTAPDGSEQAESVSFTSASGVVSVQVKNPQLWWPNGLGDQPLYQVRVLLSEGN
                     QTLDEKKYQMGLRKIELKQEPDEWGKSFTFVVNGHPFFAKGADWIPADSFPTRLTEEA
                     LEGLIRSAVATHQNMLRVWGGGFYEDERFYDLCDRYGILLWQDFIFSCSIYPLDEPEF
                     LENVRVEVVENIRRIRHRASLALWCGNNEMEQGWAEWGWQNPELDTLKAVYDKFFHHT
                     LKDWCNAEDPDNVYWPSSPSSDVPFEDPNGQIQGDAHYWEVWHRGKPFTAYRDQYPRF
                     MSEFGFQALPPLETIRTFAEESEWNMTSYIMERHQKNDSGNSLIVRQMLDTFRLPKDF
                     DALVYLSLVLQAEGIRYGVEHWRRHTNRVSGTLYWQLNDCWPVASWASLDYFGRWKAL
                     HYAARRFYAPLMLSIEDAPPKQSIFISSDLMKDWSGKVRWSLVDLDGKVLTSGEKAAS
                     VSVNGVTAVESLDFSQYLNDDLRRELAFIAELWEGDTCLARQSAFFAPTKHLKLKAPQ
                     ITSTLSAKDGVLSIEVSSKSFARLVEVSLSGADVVFSDNYFDLPANTKVTVTAPLPAG
                     WDLKKAKAAFKVRSVFDTYSN"
     gene            complement(4586..5398)
                     /locus_tag="PLR93_01435"
     CDS             complement(4586..5398)
                     /locus_tag="PLR93_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25646.1"
                     /translation="MPSEKKLYLSAKGITKTFGFGRQKTVAVDNVDLDLYEGEVVSIV
                     GESGSGKTTLSKVLLGLMNQTSGDVYFQGVKRDISTQSRKQEYWKNIQAIFQDPFSSY
                     NIFNKIDSVLLDCINMRGGKYLPREKKLEMMTEACSFVNLKFEELTNKYPFELSGGQM
                     QRLMIARIFLLQPKILLADEPTSMIDACSRATILDELMHLRKEIGMTIIFITHDIGLA
                     YYVSDSVYIMEKGKFVEGGSADEVILTPRHPYTKRLINDVPKIYEEWDLSTV"
     gene            complement(5388..6419)
                     /locus_tag="PLR93_01440"
     CDS             complement(5388..6419)
                     /locus_tag="PLR93_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25647.1"
                     /translation="MTNTMLDVRELSTKYVTRFNESVYAVDNVSLKVEEGRSLGIAGE
                     SGCGKSTLALSLMGYYFPPLHYMNGDIIINGKNISGLKPDDVRKSILGKEIAYIPQAA
                     MNALNPTRKIINFIEDVILEHEMYVTKKDIYERAKKLFETLGLPPEVLHKYPVELSGG
                     MKQRTVIAISVLLLPKVLIADEPSSALDVTSQKMVIKMLKQLMDAGFISSMIFITHEL
                     PLLYNVVDDIMVMYAGQIVEKGTSHEMVFDPIHPYSKGLMGSIIVPEKGLRDVKLTAI
                     PGVPPNLKKPPSGCRFAERCRFVRPECRVASVPLHEASNGRMYRCIIPEAELRAIYAR
                     EQEEQKNAK"
     gene            complement(6429..7679)
                     /locus_tag="PLR93_01445"
     CDS             complement(6429..7679)
                     /locus_tag="PLR93_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25648.1"
                     /translation="MKHIIQQVFRSGKFVTGFSIFSAIILIVIFYPLIITDQPLAIIG
                     QGTFFPPGIYVSVYDSMSANAYTLNLDEAAEMRIASKLNDEDRLAVKEWLVAAGIPES
                     DIDTTDTAKLLDQWFANYDSKKNIPGMTNAKRNYYIRLNNALKGLLSAEAATVMQTSP
                     QTNELEQIDTIQQTDYVNVGEVANVRVLPLGTDNFGRDVLTELVSATRVSLQIGLVAG
                     IIATIIGLTLGLLAGYIGGLVDDLIMFVTNLFIVIPPFVLLILISFSIGQEQRGAITV
                     AVVIGLTSWVWTARAVRSQVISLRNRDHVNLSKLSGHSIVHIILNDILPYIMSYVIMA
                     LILQISSGILAEAGLSILGLGPRTTEVPTLGLMMNWAMIYQAHILGNWWAYMPVLVTI
                     ALITFSMNLMNTGMDQVFNPALRD"
BASE COUNT         2006 a         1893 c         1861 g         1990 t
ORIGIN      
        1 gtcgatctgc catccactct cgcattgtag attcaatatc cagaacgcag ggcagatcta
       61 aggtcgattg aagcgtggtg gtgaggtggc tacgaatatc agctgaagaa gagccaataa
      121 ggatgtcaaa gtcgccgtct tcggtaatcc actgtttgtg ttcggggtga tagtaagcga
      181 aggcacggaa atccaaatgg atggaaacgg tcttggtctc gcccggctgg aggtccactt
      241 tggcaaagcc cttcaattct ttgacggggc gcatcagatc agacttttgg tcgtggacgt
      301 acacctgaac cgtttcttta cctgcgagtt tgcccgtgtt cttaacatcg acagtcaccg
      361 taagcccatc cacatctttg aaatttttgg aagatacttt cgcgttgcta tattcaaatg
      421 tggtgtagct caaaccgtgc ccaaatggga agagtacagg tatttctttg gtgtcgtaat
      481 aacgataacc aatgaaaatg ccttcgccat aatgaacctt gccagcgccg ccgggccaat
      541 tgatgtggga tggagtgtcg gtaagtttga gtgggaaggt ttcagcgagt ttaccgctgg
      601 ggttcacgcg cccaaataga atgtcggcaa gagccgcgcc gcccgcctgc cccatcatcc
      661 aaccttcgag cacagccgga acaccatcaa tccattcact catggcaaca ggcgcaccat
      721 tattcaacac cacaaccgta ttcggctgaa ctttggaaac agctttgatc aatgcaatct
      781 gttgaaccgt gaggtctagg tcttttcggt cgtagccttc ggactctttg aaagtgggca
      841 aagcgatgta aagtattgcc acatcagcag cctctgcgag acccactgcg tcatcgatca
      901 taccttgctg gaaggagtta tcggtggggt agccttcggc gtaggtcaac tcggcgtttt
      961 cggcgcgaga ttgaagttcc ttataaggca tggcgacttt ggtcgggttg atgtgtgagc
     1021 tgccaccgcc ttgaaaatgc gcggactctg ctgagcgccc aatgactgcg atatgttgat
     1081 gatctttcaa cggtaataga ccatcgttct tcaacagcac cacaccttct gtggcgatct
     1141 ttcgtgccag ttcatggtgg gcatccacat caaaggagcc gttccctttg ggagtttctt
     1201 tagatttaaa aacgatgcgc aaaatacggc gaacggattc gtccaatgtg gcttcgttca
     1261 actcaccaga gcgaaccgcc tcgaccacag ctttgacacg caagtcctgc ggacccggca
     1321 tttcccaatc caaacccgct ttgagagccg cgacccggtt ccgcaccgcg ccccagtcgg
     1381 atacgaccaa accttcaaag ccccattcat ctttgaggat tttggtaagc aattgatgat
     1441 gttcagaagc aaaggtgcca ttgaccttgt tatatgagca catcaccgtc cacggttgag
     1501 cttgtttgac agctttctca aacgcgggta gatagatctc acgcagggtg cgttcgtcaa
     1561 tctcggcact gatgctgaag cgctggaatt cttgattatt ggcggcgtaa tgtttgagcg
     1621 aagttcctac gcctttagat tgcacgccat tgatgaagtt agcagccaat tcgcctgcaa
     1681 ggaaggggtc ttcggagaaa tattcaaagt tacgcccatt gagcggagac cgcttcatat
     1741 ttacaccggg accaagcaag acatccacat tgagagcgat acattcttca gcgagggcaa
     1801 tgcccatttg cttgatcagg tccacatccc aggtagatgc ggtgcaggat gcggttggga
     1861 agcaggtagc aggcaaactc ttttgtccca tcgagtggac atccgcgaca cgacgcactc
     1921 catgcggacc atcggagaca atcatttcgg gcagccccaa acgctcgaca ggcgtagtag
     1981 accagttgct ggctccagtg caaagagccg ccttctcttc aagggtcatt tgagaaatga
     2041 tggatttaat atctgtagcc atagtctttt cctttttaat tggaatacgt atcaaataca
     2101 gagcggactt tgaatgccgc cttggctttt ttgagatccc aacctgcggg caaaggtgct
     2161 gttaccgtca ccttggtatt tgcaggtaga tcgaaatagt tatcgctgaa gaccacatct
     2221 gcaccgctta ggctaacttc cacaaggcgc gcgaacgact tagatgaaac ttcaatggaa
     2281 agcactccat ctttcgcact caaagtggat gtgatctgtg gagcttttaa tttgaggtgc
     2341 ttggtcggtg caaagaaagc ggattgacga gcaaggcagg tgtcaccttc ccatagctca
     2401 gcaatgaaag caagttcacg gcgcaagtca tcgttcaggt attgagagaa gtctaatgac
     2461 tcaacggctg tgactccgtt taccgaaacc gaggcagctt tttcaccaga ggtcagcacc
     2521 tttccgtcca agtccaccaa agaccagcgg accttaccgc tccaatcttt catcaaatca
     2581 ctactgatga agattgattg cttgggtggt gcatcttcaa tggaaagcat caatggcgca
     2641 tagaagcgac gggcagcgta gtgcagagcc ttccaacgcc caaaatagtc aaggcttgcc
     2701 cacgaagcga cgggccagca atcgttcaac tgccaataca aggtgccaga gacgcggttg
     2761 gtatgtctgc gccaatgctc tacaccatag cgtatccctt cggcttgcaa cacaaggctg
     2821 agatacacca acgcatcaaa gtctttggga agcctgaacg tatcaagcat ctgccgaacg
     2881 atcaacgagt tgccgctgtc attcttctgg tgacgctcca tgatgtaaga ggtcatgttc
     2941 cattcggatt cttcagcaaa ggtacgaata gtctcaagcg gtggaagcgc ctgaaaacca
     3001 aactcactca tgaagcgcgg atattgatcg cgataagccg taaatggttt gcccctgtgc
     3061 catacttccc aataatgtgc atcaccttga atttgtccgt tcgggtcttc aaagggaaca
     3121 tcagaggaag gcgaactggg ccagtacaca ttgtcggggt cttcggcatt gcaccaatct
     3181 ttcaaggtgt ggtggaagaa cttgtcgtac acggctttca aggtgtcaag ctctgggttt
     3241 tgccaacccc attcagccca gccttgttcc atttcgttgt tgccgcacca caatgccaaa
     3301 gaagcacggt gacgaatgcg gcgaatattc tccacaactt ccactcgcac gttctctaaa
     3361 aattcaggct cgtcgagcgg gtaaatgctg caggagaaaa taaagtcctg ccaaagcaaa
     3421 atgccgtagc ggtcgcacaa atcgtagaaa cgctcgtctt cataaaagcc accaccccac
     3481 acccgcagca tattttgatg cgtcgccacg gcagaacgga tcaacccttc aagcgcttct
     3541 tccgtcaaac gagttgggaa ggaatcagca ggaatccaat ctgcgccttt ggcgaagaag
     3601 ggatgtccat tgactacaaa ggtgaaggac ttgccccatt catccggttc ttgcttaagc
     3661 tcaatcttgc gcaaacccat ttggtatttc ttttcgtcca aagtttggtt gccctcactt
     3721 aagagcacac gtacttggta aaggggttgg tctcccaaac cattgggcca ccacaactgc
     3781 ggattcttca cttgcacaga aacaactccg ctggcagaag tgaaggacac gctttcggct
     3841 tgctctgaac cgtctggagc ggtaacaagt aaggtcgcag aaagaggcgc gtttccattt
     3901 tccacttcca cacgagcaga aatggtcacc ttgccattgt catgtttttg gcgaagatga
     3961 acttcagaga tacgagccga gtcataggct tcaaggtaaa tatctttcca gatgccgata
     4021 ggcggaagct gcggacccca atcccagccg aaatgacatg gagctttgcg caggtgtggt
     4081 ccaccggcga tggcttgcga aacgccgttc atggggcgtg ccgcattttt ctcggttaca
     4141 aacttgacgg gtgatttgaa cgtaatggtc agctcattgc cttcagcttt gagcaaggac
     4201 tttatatcaa aacgatattg gcggaacatg ttattggttt cgccaagcgc ttttccattg
     4261 agtgtgagca acgcaagcgt atccaaccca tcacaaacaa gccagatatt tttgcgctga
     4321 aagacatctg cctgcacatt gaaggtggtg tgatattccc agtcgctttc ggcaacccag
     4381 gcaactcgtt tttcattatc gcctacaaat gggtctggga tgcgtgcaag cgccatgaga
     4441 tccgtgtgaa ctccgcccgg aacctgggct gggagccatt ctatagaacc caactggcga
     4501 aactgccata ggcttgttag agattgtttt tgcattggca ttcttcctga atgttttctt
     4561 ctttagattt ttattgctcg tgcttttata ctgtggagag atcccattcc tcgtagatct
     4621 tcggcacatc gttaatgagg cgtttggtat aaggatgccg cggagtgaga atcacttcat
     4681 ctgccgagcc gccttccaca aatttgcctt tttccataat atagacacta tctgaaacat
     4741 agtacgccag accaatatca tgcgtgatga agatgatggt catgccgatc tctttgcgga
     4801 ggtgcatcaa ctcatccaaa atggttgcac gagagcaggc atcgatcatg gaggttggtt
     4861 catcggcaag gagaattttg ggctgtaaca aaaagatgcg ggcgatcatg aggcgttgca
     4921 tttgtccgcc ggaaagttcg aagggatatt tattggtcaa ctcttcgaac ttgaggttca
     4981 cgaagctgca tgcctcagtc atcatttcta atttcttttc acgcggcaaa tattttccgc
     5041 cgcgcatgtt gatgcagtcc agcaagaccg agtcgatctt attgaagatg ttataggaag
     5101 agaacgggtc ttggaagatg gcttgaatgt tcttccaata ttcctgtttt ctggactggg
     5161 tgctaatgtc gcgcttgacg ccttggaagt acacatcgcc cgatgtttgg ttcatcaatc
     5221 ccaatagcac tttagagagg gtggtcttgc cgctgcccga ctcacccaca atggaaacaa
     5281 cttcaccttc atacaagtcg aggtctacat tatccaccgc cactgttttt tggcgtccga
     5341 acccgaatgt tttggtgatg ccttttgcgc tcaggtagag tttcttttca cttggcattt
     5401 ttctgttcct cctgctcacg agcatagatt gcgcgcagtt cagcttctgg gatgatacag
     5461 cgatacatac gtccattgct cgcttcatgt aaaggcacac ttgcgacacg gcattccggt
     5521 cgaacaaagc gacagcgttc ggcgaagcga caacctgaag gcggcttttt gaggttaggt
     5581 ggcacgccag gaatggcggt cagtttgaca tcgcgcaaac ctttttcagg aacgatgatc
     5641 gaacccatca atccttttga ataaggatga atgggatcga agaccatttc atgcgaagtc
     5701 cccttctcca cgatctgacc ggcgtacatg accatgatgt catccactac gttataaagc
     5761 agaggcaact catgcgtaat gaaaatcatg gagctaatga aaccggcatc cattaactgt
     5821 ttgagcatct taatgaccat tttttgcgag gtcacatcga gtgctgaaga gggttcatct
     5881 gcaatcaaca ctttgggcaa aagcagtact gaaatagcga tcacagtacg ctgtttcata
     5941 ccgcctgaaa gttcaactgg gtatttatgt aaaacttcgg gaggaagacc caatgtttca
     6001 aagagttttt tcgcgcgttc gtaaatgtcc ttcttggtaa catacatctc atgttcaaga
     6061 atgacatctt caataaagtt aatgatcttg cgtgttgggt ttagagcgtt cattgccgct
     6121 tgggggatat aggcaatctc tttaccaaga atggacttgc gcacatcgtc tggcttcaaa
     6181 ccggagatat tcttcccatt aataatgatg tcaccattca tgtagtgcag aggcggaaag
     6241 taatatccca tcagactgag ggcgagggtt gattttccac agccagattc ccctgcaatc
     6301 ccaagcgaac gtccttcttc tactttaagt gacacattat caaccgcata tacactctca
     6361 ttaaagcggg taacatattt tgtagatagt tctctaacat ctaacattgt gttcgtcata
     6421 ttaatctcct agtctcgcag ggctgggttg aagacctgat ccataccggt attcatcagg
     6481 ttcatcgaga aggtaatcag cgcgatggta acgagcaccg gcatataagc ccaccaattg
     6541 ccgagaatat gtgcctgata aatcatcgcc cagttcatca tcaatcccag agtggggact
     6601 tccgtagtgc gcggtcccaa cccaaggata gaaagccccg cctctgccaa aataccagag
     6661 gagatttgca aaatgagcgc catgatcaca tatgacatga tgtatggcaa gatatcattg
     6721 agaatgatgt ggacaatgga atgcccagag agttttgaaa gattgacatg gtcgcggttt
     6781 cgcaaagaaa tgacctgcga acggacagca cgtgctgtcc acacccatga ggtaagcccg
     6841 atcactactg caacagtaat tgcgccacgt tgctcttgcc caatactaaa tgagatgagg
     6901 atcaataaca cgaacggtgg gatgacaata aaaaggttcg tgacaaacat gatgaggtca
     6961 tctaccaacc cgccaatata gcccgcgagc aagcccaagg tcagaccgat aatggtggca
     7021 attattcctg ccacaagacc aatctgcaaa gagacacggg ttgcagagac cagctcagtt
     7081 agcacatctc tgccaaagtt atctgtacca agcggtagca ctctcacatt ggcaacttcg
     7141 ccgacattca cataatctgt ctgttgaata gtatcaatct gctctagttc attggtctgc
     7201 ggactggtct gcatcaccgt cgccgcttca gcagaaagca accctttaag ggcgttgttg
     7261 aggcgaatgt agtaattacg tttagcattc gtcatgccgg ggatgttctt tttcgagtcg
     7321 taatttgcga accactgatc cagaagtttt gccgtatccg tagtatcaat gtcgctttct
     7381 ggaatgccag cagcgactaa ccattctttt acagccagac gatcttcgtc atttagttta
     7441 ctagcaatac gcatttcagc tgcttcatct aagttgagtg tgtatgcgtt cgcgctcata
     7501 ctatcgtata cacttacgta aattcctgga ggaaagaacg taccttgacc aataattgcc
     7561 aaaggctgat cggtaatgat caatggataa aagatcacaa tcagaatgat ggctgaaaaa
     7621 atagagaagc ctgtaacgaa cttacccgag cgaaaaacct gttggattat atgtttcatg
     7681 ctggtttctc cacttagtca gattgtgcag ctttgatgcg cggatcgatc aagccataca
     7741 agatctcaat
//
LOCUS       DAOQFT010000031         7745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4527_length_7745_cov_6.830689, whole genome shotgun sequence.
ACCESSION   DAOQFT010000031 DAOQFT010000000
VERSION     DAOQFT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_7745_cov_6.830689"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLR93_01450"
     CDS             <1..956
                     /locus_tag="PLR93_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNH25649.1"
                     /translation="PLPFSGVIISFNMSFIELFNTFANNLLPIMLIAAAGYILGKTLT
                     VDSRTIGRMVFYIFSPLLVFNLMVTSQLNLKQALTTVGYTVAVIAIMGLLAFIFGKIF
                     QLSRPHLLAVIITVAFGNTGNYGLPLVKFAFGDEALAVASIFYVTTTILFNTVGVVIA
                     SLGHLDLKSAILGVFKLPILYAVTIALILKGFGIAIPLPLSRTIEIAANGAIPAMIIL
                     LGLELTRIEWTQSLRATGVGVAAKLLIGPLVGLLLASLFGLQGHTRQGSVIEAAMPAA
                     VATTVVATEYKLEPSLVTAIVFFGTILSPITLTPLIVFLTR"
     gene            962..2194
                     /locus_tag="PLR93_01455"
     CDS             962..2194
                     /locus_tag="PLR93_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNH25650.1"
                     /translation="MSATRQRILQVFMVSLLVATLSLKPNQAQAQEGIVVENPNATPD
                     FGKTITFTAKIKASNPIQQASLLFRGVNEETTRVETLQVAEDGSVQFVYDASQNVFPP
                     FSWVVYWFQATLSDGKTYTSESNRVQYVDNRFPWRDVTQANVTIHWYAGDDAFGASAL
                     DSAGAGMLGMKDFIPLSLTEPIHIYIYSNVQDLGDTLMLGGREWAGGHAHADLGVVLV
                     AIAPGANQFTEMESLIPHELAHVMLYRALGDTYYKQPAWLIEGIASMVELYPNPDYAR
                     ALEIASVNDTLIPFSDMCASFPADSGSAFLAYAQSQSFVTYIRDSFGVSGLSRLISTY
                     GDGFDCDLGITKALGASLNQLDSRWRENVLGQNQVGAALRNLLPFLLLLLLVLIVPIW
                     GAVDVMRRRRKKYAAKSK"
     gene            2175..2477
                     /locus_tag="PLR93_01460"
     CDS             2175..2477
                     /locus_tag="PLR93_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNH25651.1"
                     /translation="MQPNPNEPVRVHIWVKGRVQGVGFRAHVEYSARQIGGLSGWVRN
                     VGDDTVEAIAEGQREQVERLIEAMKQGPRMSRVDEAKIDWESPTGEYNGFRVKSSA"
     gene            2481..4106
                     /locus_tag="PLR93_01465"
     CDS             2481..4106
                     /locus_tag="PLR93_01465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH25652.1"
                     /translation="MFSNPYSPYLFTSAITSFVMAMLLWRRRIAPGATPLSVLLGLTS
                     VWALASALLLESSSTLLRELWFDILSISAMMTTPMLLIFCLEYTERQNFLSKRLLLFV
                     YLFPILNAILCVTTNYHHLFYAEVDFSYQPANFLALVPGPFYWIYTVYSYGIALFIFV
                     LILTNFFQTSSTYRKQTATILLGLLFPFVGNVIYTLLLGVGKGAFDPTPMLFTVMGFF
                     LVCGLLWFQLFDVIPVARHTLVEHMQDGVIVVDVKNRIVDVNPSALKWLNWEGKSSPI
                     GKNVEELLTSWFEQFANLPIDLYVQTEVHKEGTPGLYFDLRVEPIYNRQNVLTGRLIV
                     FRDITRQKMSEKALLEAHDRLRLHLKEIELLQSELREQVIRDPLTGLFNRRYMEETLE
                     REISRAIRENISIGVCMADIDQFKSFNDEHGHKAGDLILKKLSEILTNYSRAEDVVCR
                     YGGEEFLILMPGAELDAIARRAEDWRRAFEQAKVEFDGKQLSTTLSMGIAMFPQQART
                     SDDIVKLADEAMYLSKHNGRNQVSIAQTWGEKR"
     gene            complement(4103..4810)
                     /gene="radC"
                     /locus_tag="PLR93_01470"
     CDS             complement(4103..4810)
                     /gene="radC"
                     /locus_tag="PLR93_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNH25653.1"
                     /translation="MTDSTNSKPTYRISDLHEADRPRERLAALGPQALTNAELLAILL
                     RVGVQGENAVEVGQRLLKKFNGLNGLHRAPFKELMDQHGLGEAKAAQIKAAIELGRRL
                     TLETPQERPAINSPADAAALIQYEMSALEQEHLRVILLDRRNRVLEVVEVYKGSVNSS
                     QVRVGELFKDAIRANASAIIVAHNHPSGDPTPSPDDVAVTRAIVQAGKLLDVDVLDHL
                     VIGQAKWVSLKERGLGF"
     gene            complement(4895..5980)
                     /locus_tag="PLR93_01475"
     CDS             complement(4895..5980)
                     /locus_tag="PLR93_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25654.1"
                     /translation="MKSFLRTLVLTLILTALITACGLPAGTADPDVVSTAIAQTQMAQ
                     VLAQATVNSNTLTAMPATPTPGPTVEYVTLTEEELAALIDQAVAEAIAATEQTTTNVY
                     YVTEDDTVTTEEVEYVYSYYYYADYYVDYAEELMAEYYNLYSDLAYEMIDELNAIESE
                     LNQMNDTLSSIDQSLQQISSTLEQGLELAEDTITQLETAAQNAQTNAQELKSQAQDMM
                     TVLQADQQGRLDQIGQIQPDNIPTDKLSALQTAFSFVDFANQALSDNKLTRDELTNLM
                     QLGKNAQAGFQQFGGADGVGPDVTQFAGKFDEIGGQFARGEINHGRENLHNFEGSLGN
                     RPSFGNGQGGPSGNGPGGGGGLPGPRP"
     gene            complement(6002..7315)
                     /locus_tag="PLR93_01480"
     CDS             complement(6002..7315)
                     /locus_tag="PLR93_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNH25655.1"
                     /translation="METNLVLEQPVYKTSTFEFLWNVFRNPLHALTDLAHEQGDIARV
                     KLRKRDLFLLSHPAFIEQVLVRQADNFVKGPSLQRARIILGEGLLTSEGDEHLTQRRT
                     LQPAFHRHKMEEYIPLMNENTMAHIANWQDGERADMSEGMMRLTLNIALWSFFGNAPS
                     GTVDKVSDAMKTLVRLFPLAQMPLPESARSLFPSFKEAKADLSEVTEALLKNPNSEYA
                     KRALVNILKENHKDQFTDEQVHAHALTFLLAGHETTALLLAWCWDLLAHHPMAQAILQ
                     AEVDSVLGDRLPTPEDIQKLPYTRAVVKETLRMRPPAWAMGREALHDCEIGGQFIPAG
                     STVIMSQWVTHHDSRFYYEPLHFRPERWFDLEGTSTPRYSFFPFGGGNRVCIGEHFAM
                     TEAIAILAVVARRWNISPVQRELATPKPSVTLRPEKNLNVIVTKR"
     gene            complement(7459..>7745)
                     /locus_tag="PLR93_01485"
     CDS             complement(7459..>7745)
                     /locus_tag="PLR93_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH25656.1"
                     /translation="AQGKPQLHPDIAKRLMARAPMPDDPFDSLTERERGILKLLAKGK
                     SNKEIAGELSLTEMTVKGYVSDMFVKLGVNDRTQAALMAVRYGLVLPEEL"
BASE COUNT         1872 a         1939 c         1844 g         2090 t
ORIGIN      
        1 cgcccctacc tttttcgggg gttataattt cattcaacat gtccttcatc gaactcttca
       61 acacctttgc aaacaacctg ctgcccatca tgctcatcgc tgcggctgga tacatcctcg
      121 ggaaaacgct caccgtcgac tcgcgcacca ttgggcgtat ggtcttttac atttttagcc
      181 ccttactcgt tttcaactta atggtcacca gccagttaaa cctgaagcaa gccctcacta
      241 cagtgggata cacagttgcc gtgattgcaa ttatggggct gcttgctttc atcttcggga
      301 aaatctttca acttagccgc ccacacctct tggcggtcat tatcacggtc gcatttggaa
      361 acacgggcaa ctatggcttg cccttggtta aattcgcatt tggcgatgaa gcattggctg
      421 ttgcttctat cttttatgta acgaccacca ttcttttcaa taccgttggg gtggtcattg
      481 catcgctcgg gcatttagat ttgaaatccg ctatactggg agtattcaag ctgcctattc
      541 tttacgctgt gaccattgca ttaatcctca aaggctttgg catcgccatt ccactgccgc
      601 tgtcacgcac catcgagatc gccgcaaacg gagccattcc tgccatgatc atcttgctcg
      661 gcttggagct cacccgcatc gaatggacgc aaagcctgcg cgcgactggc gtgggcgttg
      721 ctgctaagtt attgatcgga ccgttggttg ggttattgct ggcaagcctg ttcgggttgc
      781 aagggcatac ccgtcagggc agtgtcatcg aagccgccat gcccgccgct gtggcaacaa
      841 ccgttgttgc taccgaatac aaacttgaac cgtctcttgt gacagcgatt gttttcttcg
      901 gcaccatact gagtccgatt acccttacac cgttgatcgt tttccttacg aggtaatgaa
      961 catgtccgcc acacgtcaaa ggattttgca agtcttcatg gtctcacttc tcgtggcgac
     1021 cctttctctg aaaccgaacc aagcccaggc gcaagaagga atcgttgtcg agaatccaaa
     1081 tgcgaccccc gatttcggaa aaacgatcac attcacagca aagatcaaag catccaatcc
     1141 tattcaacaa gcatcgctct tgttccgcgg ggtcaacgaa gaaacgaccc gtgtagaaac
     1201 cttacaagtt gcagaagatg gctcggtaca attcgtctat gacgcttcac aaaatgtttt
     1261 ccctccattc agttgggtcg tctattggtt tcaagcaaca ttaagtgacg gcaaaactta
     1321 caccagcgaa tcaaatcgcg ttcaatatgt tgataatcga tttccatgga gagatgttac
     1381 acaagccaac gtcaccattc attggtatgc cggcgatgat gcttttggtg caagcgcatt
     1441 agatagtgcc ggtgcaggca tgttggggat gaaagatttt attccgctct cacttaccga
     1501 acccattcat atttatatct actccaacgt ccaagacctt ggcgatacgc tgatgctcgg
     1561 cgggcgcgaa tgggcaggcg gacacgctca tgcagacctt ggcgtggtat tggttgcaat
     1621 tgcgcccggc gccaatcaat tcaccgaaat ggaatctcta atcccacatg aacttgcaca
     1681 tgtcatgcta tatcgcgcgc ttggcgatac ttactacaaa caacctgcct ggctgatcga
     1741 aggcattgcc tccatggtgg agctttaccc caaccccgat tatgcgcgtg ctttggaaat
     1801 tgccagtgtg aatgatacct tgattccatt ttcggatatg tgcgcctctt tccccgccga
     1861 ttctgggtca gcatttctgg catatgcaca atctcaatcc tttgtcacct acatccgcga
     1921 ctcgttcggg gtctcgggtc tctcacgcct gattagtact tacggtgacg gcttcgactg
     1981 tgacctcggc attaccaaag ccttaggtgc ttcgctcaac caattggatt cacgttggcg
     2041 tgaaaatgtg ctcgggcaaa atcaagtagg cgcagcgtta cgaaacttat tgcccttcct
     2101 acttttactt cttcttgttt tgatcgtacc aatctgggga gccgttgatg tgatgcgccg
     2161 aaggagaaag aaatatgcag ccaaatccaa atgaaccagt ccgtgtacac atttgggtga
     2221 agggacgggt gcaaggggtt gggtttcgtg cacacgtaga atattcggcg cgccaaattg
     2281 gagggttaag tggctgggtg cgcaatgtag gggatgatac agtggaagcc atagctgaag
     2341 gtcaacgaga acaggttgaa cgtttgatcg aggcgatgaa acaaggtccg cgcatgtcac
     2401 gggtggatga ggcaaaaatc gattgggaat cgcccacagg ggagtacaat gggtttagag
     2461 taaaaagcag cgcataagtt atgttttcaa acccatactc cccctaccta tttacctcag
     2521 ccatcacctc atttgtgatg gcaatgttgc tgtggcgtcg acgcatagct ccgggggcga
     2581 ctccattatc cgtcctcttg ggtctcactt cagtttgggc gttggcatcg gctttgttgc
     2641 tagagtcgtc cagtacgtta ctcagggagt tatggttcga tatattaagc ataagcgcaa
     2701 tgatgaccac ccctatgctg cttatctttt gcttggaata cacagagcgc caaaacttct
     2761 taagtaaaag attgttactc tttgtctatt tatttccgat tcttaatgcc attctgtgtg
     2821 tcacaacgaa ttaccatcac ctgttctatg cagaggttga tttttcatac cagcctgcta
     2881 attttcttgc tttggttcca ggtccatttt attggatata caccgtgtat tcctacggga
     2941 tagcgctttt tatatttgtt cttatcctta cgaatttttt tcaaacctct tcaacttatc
     3001 gcaagcaaac agccactatt ctacttgggc tattgttccc attcgttggg aacgtgattt
     3061 acactctgct tcttggggtt ggcaaaggtg cttttgaccc cacacctatg ttgtttactg
     3121 tgatgggatt cttccttgta tgtgggttgc tctggtttca gttattcgat gtgatcccag
     3181 ttgcgcggca tacactggtt gaacacatgc aagatggcgt gattgtagtg gatgtaaaaa
     3241 atcgcatcgt agacgtgaac ccatccgcct tgaaatggct gaattgggaa ggcaaatcat
     3301 cccctattgg gaaaaatgtt gaagaacttc ttacttcgtg gtttgaacaa ttcgcaaact
     3361 tacccatcga cctttatgtt caaacagaag tacataaaga aggcactcca gggttgtatt
     3421 ttgacctgcg tgtagagcct atctacaata ggcaaaacgt tctcacgggt cggctgatcg
     3481 tgtttcggga tatcacccgc cagaaaatgt ctgaaaaggc tttattggaa gcccatgacc
     3541 gcttaagact ccatctgaaa gagattgagc ttctgcaaag tgagttgcgc gaacaagtca
     3601 tccgcgaccc gctgacgggg ttgttcaacc gtcgttacat ggaagaaaca ttggagagag
     3661 aaatttcgcg agccattcgc gagaatattt caatcggggt ttgtatggca gatattgacc
     3721 agttcaagtc tttcaatgac gagcatggtc ataaagctgg tgacttgatc ttgaaaaagc
     3781 tatcagagat attaaccaat tactcgcgcg ccgaagatgt cgtctgtcga tatggaggcg
     3841 aagagttctt aatcctcatg ccgggcgcgg agttggatgc cattgcccgc cgcgccgaag
     3901 attggcgcag agcatttgag caagcaaaag tagaatttga tgggaagcaa ctctctacca
     3961 cgttatccat gggcattgcc atgttccctc aacaagcgcg gacaagtgat gacatcgtca
     4021 agttagcaga tgaagccatg tatctttcta aacacaatgg cagaaatcaa gtaagtattg
     4081 cacaaacatg gggcgaaaaa cgctagaaac ccaaaccgcg ctctttcagt gaaacccact
     4141 ttgcctgacc aatcaccaaa tggtcgagca catccacatc caatagtttc ccggcttgta
     4201 caatggcacg tgtcacagct acatcatctg ggctgggagt gggatctccg ctcggatgat
     4261 tatgtgccac aataatagcg gaagcattgg cacggattgc atctttgaat aattccccta
     4321 ctcgcacttg tgatgaattg accgaccctt tatagacctc aacaacttcc agcactcgat
     4381 ttcttctatc caacaatatc actcgcaaat gttcctgctc caaagcggac atttcatatt
     4441 ggatcaatgc cgccgcatct gccgggctat tgatcgcggg tcgttcctga ggcgtttcaa
     4501 gagtcagcct acgccccagt tctatggcag ccttgatctg cgccgctttg gcttcaccca
     4561 acccatgttg atccatcagt tccttaaatg gtgcacgatg taacccattc aagccattaa
     4621 actttttcaa cagacgttga cccacttcta cagcattttc accctgcacg cccactcgca
     4681 acaaaatagc gagcagctct gcattcgtca gcgcctgcgg acccaatgcc gcaagccgct
     4741 cacgcggacg gtctgcctcg tgcaagtctg agatacgata ggttggtttt gagttggtgg
     4801 agtctgtcat ttcgcctccc ttgagataag ttgaccaatt ttacagccaa ttcagaaaaa
     4861 gtctgctgac atttttatca gcagactttt tcatttatgg gcgcggaccg ggcaaaccgc
     4921 cgccacctcc aggtccattt ccgctgggtc caccctgacc gttcccaaat gaagggcggt
     4981 tgcctaagga accttcaaaa ttatgcaagt tttcacgtcc atggttgatc tcgccgcgtg
     5041 caaactgccc accgatttca tcgaatttgc ctgcaaattg tgtgacgtca ggtccgacac
     5101 catctgcgcc accaaattgc tggaaaccag cttgggcgtt cttgccgagt tgcataaggt
     5161 tcgtcaattc atcacgggta agtttattat cgctgagtgc ttggtttgca aaatccacaa
     5221 aggagaaagc cgtttgcaat gcagacagtt tatcggttgg gatattgtcc ggctggatct
     5281 gaccgatctg gtccaaacga ccttgctggt cagcttgtaa aacagtcatc atatcctgtg
     5341 cttgagattt caactcttgc gcattggtct gggcattttg agcagcagtt tcaagttgag
     5401 taatggtatc ttctgccaat tccaatcctt gttccagtgt ggaagaaatc tgttggagag
     5461 attggtctat ggagctaagc gtatcattca tctgatttag ctcagattca atggcgttta
     5521 attcatcgat catttcataa gccaggtctg aatacaagtt gtagtactct gccattagtt
     5581 cctcagcata atccacatag taatcggcat agtaataata gctgtataca tactccacct
     5641 cttcagttgt gaccgtgtcg tcttctgtca cgtaatacac attggtcgtg gtctgttcgg
     5701 tggcggcgat agcttctgca acggcttggt caatgagcgc ggcaagttct tcttcagtga
     5761 gtgtcacata ctcaaccgtg ggtcccgggg ttggagtagc gggcatagct gtcaatgtat
     5821 tggaattgac tgtagcttga gcaagaactt gcgccatttg tgtttgagca attgcggtgc
     5881 tgacaacatc cgggtcggcg gtgccagcgg gtaacccaca ggcagtgata agagcagtga
     5941 gaatcagggt caatacaagg gttcgaagga aagatttcat gttatatctc caatttagtt
     6001 tctatcgttt cgtcacgatc acatttagat ttttttcagg gcgtagtgtc acactgggtt
     6061 tcggtgtggc aagttcgcgc tgaaccggtg agatattcca acgtctcgca acgacagcca
     6121 gaatggcaat ggcttcggtc atggcgaaat gctcaccaat gcagacgcga ttgcctcctc
     6181 cgaaggggaa gaaactgtaa cggggagttg aagttccttc gaggtcgaac cagcgttcgg
     6241 ggcggaagtg aagcggttcg taataaaagc gtgagtcgtg atgggtcacc cactggctca
     6301 taatcacggt cgaacccgcc gggatgaact gcccgccaat ctcgcaatca tgcagggctt
     6361 cgcgccccat cgcccaagcc ggtgggcgca ttcgcaaggt ctctttcacc accgcgcgtg
     6421 tgtatggaag tttttgaata tcttcgggcg tgggcaaacg gtcacctaaa accgagtcga
     6481 cttccgcttg caaaatagct tgcgccatcg ggtgatgcgc gagcaggtcc caacaccagg
     6541 caagcagcaa agctgtggtt tcatgcccag ccagcaagaa cgtgagcgca tgcgcgtgga
     6601 cctgctcatc ggtaaattgg tctttatgat tctctttgag gatattgacc aaagcccgct
     6661 tggcatattc agagtttgga ttcttgagca acgcctctgt gacttcactc aggtctgctt
     6721 tggcttcctt gaagcttggg aataagctgc gagcggactc tggcaaaggc atttgcgcca
     6781 gcggaaagag acgcaccaag gtcttcatcg catcgctgac tttatccaca gtgcccgaag
     6841 gtgcattgcc aaagaaagac caaagtgcaa tgttgagcgt cagacgcatc atgccttcag
     6901 acatatctgc ccgctcgcca tcttgccagt tcgcaatatg tgccatcgta ttctcgttca
     6961 tcaagggaat gtattcttcc atcttgtgac gatgaaacgc gggttgcaag gtgcgacgtt
     7021 gagtaaggtg ttcatcccct tcgctggtga gtaacccttc gcctaaaatg atgcgtgccc
     7081 tctgcaagct gggacctttc acaaagttat ctgcctgccg caccaagacc tgttcaataa
     7141 atgccgggtg gctcagtaga aagaggtcac gcttgcgcaa tttgactcgt gcaatatcac
     7201 cctgttcatg cgcgagatcg gtcaacgcat gcaacgggtt gcgaaagaca ttccataaaa
     7261 attcaaaggt ggatgttttg taaacgggtt gttcaagaac taaatttgtt tccatcggct
     7321 atctccttca agtgataacc aaaggatact tttttgaacc gattaagtcc gcccgcgaag
     7381 gtggggaacc tctcccccta tttggtgggt atataaaaaa agtgacagtc accttaaagg
     7441 tgactgtcac tttgagttct acaattcttc cggcaaaacc aacccatagc ggaccgccat
     7501 cagcgccgcc tgagttctat cgttcacccc caacttcacg aacatgtcac tgacatagcc
     7561 cttcaccgtc atctcggtca gcgataactc gccagcaatt tctttattgc tcttaccctt
     7621 tgccagcaat ttcaaaatgc cgcgctcacg ttcggtcaac gaatcaaacg ggtcatcggg
     7681 catgggagcg cgcgccatca ggcgcttcgc aatatcaggg tgcaattgcg gtttgccttg
     7741 cgccg
//
LOCUS       DAOQFT010000032         7651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4600_length_7651_cov_8.907978, whole genome shotgun sequence.
ACCESSION   DAOQFT010000032 DAOQFT010000000
VERSION     DAOQFT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4600_length_7651_cov_8.907978"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLR93_01490"
     CDS             complement(<1..365)
                     /locus_tag="PLR93_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25657.1"
                     /translation="MRIAKHFRKDQETLQRFLDVFGAGSAMLGGGNKNVQPGFFIHGS
                     TFIHEYVDGNFFRKEELLMKTLEDAGFPVNSGVIGNMKDEHAKCRQAADLLSKAASDW
                     QGGDAGARIEVGWAASEYTS"
     gene            complement(618..1172)
                     /locus_tag="PLR93_01495"
     CDS             complement(618..1172)
                     /locus_tag="PLR93_01495"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNH25658.1"
                     /translation="MNNHSQEQFKNLFLEKMETFTRNEWELLRHIMDRRHVTRNVNKE
                     FDDQLTFGQRLADNVAKFGGSWTFIIIFGLILLTWVFLNSVVLARLNDAFDPYPYILL
                     NLFLSMLASIQAPIIMMSQNRQATKDRMDAAHDYEVNLKAEMEIETLHQKLDELRDKQ
                     WAELVEMQQEQIRLLTKLLEEKSK"
     gene            complement(1165..1761)
                     /locus_tag="PLR93_01500"
     CDS             complement(1165..1761)
                     /locus_tag="PLR93_01500"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH25659.1"
                     /translation="MWRTIPTMHLEQLITHFGYPALLLGLLLEGETVLVLAAFMAHRG
                     YLDLPLVILLGGLVAFASDQFFFWMGRIQGNTFLANRPAWQPHVEKAKALLDRNSTLL
                     FLSVRFMYGLRTVLPFVLGMSKLNTKKFVLLDFIGAFLWAITFGLAGHFIGHVMTLIF
                     DDVKEHELPVMIGIALIGIGIWLYRRNNNKDKDGKDHE"
     gene            1863..4544
                     /locus_tag="PLR93_01505"
     CDS             1863..4544
                     /locus_tag="PLR93_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH25660.1"
                     /translation="MLSENSELAHQGSTEVQRARKTARSTFFTALKFMATRLLTVLLT
                     IGLGTFITIVAANGGGAIDNAEKSRINAVTLRSVDPFATSAEELTKLRADMEEKEGLN
                     LPFLPRHMYWTYKALTLNWGEVNDRVKFHPRYTTSNMNAEDSRVILMQYLPNTILLIG
                     AGFFFLFIAGIPFALHLSQNPGGRLDKLIGTLTPLSSIPGWVIGFILILVFAVGLKIL
                     PVGRMIDSIPPESSFEYVLTVIRHMVLPVTAILLSSAFTLIFSWRTYFLAYSHEDYVD
                     VGKAMGLPQKTFRRNYILRPVLPAIITSFSLSMVGFWQLITTVEFVFDWPGIGSLFVR
                     ALPHFLSERMYPGEIPIIIGTMVIFAYLLGFTVFMLDIAYLVTDPRIRVTSGGSSQRL
                     VAKRTKRKWTFPTFKRPEFSWTETKQNFSDTKAQWAENWKKFLALLSELRGYPSAIIG
                     FSIVGLLVLTSIYTVIALPYRQIGRDWFLLSVKPRSVVPKLALPSWVNFFRENDLPST
                     ILIDSRTDSVERTETVLNETTKTITLTYTFDYPYTEFPKDMVIYYYPTYQEKVPFASM
                     ILTTPDGRELELRGEMTSFEKNYEPGSVLNINKILKQNPNWKEWFSIDEHYPTPAFYL
                     LFADPNAKTAKAVPGTYILTINAVGFEKDWDLDAQLVIFGQVYGMAGTDYMRRDLMTP
                     LLWGMPIALIFGLLGSIVITVGSAIVGAAAAWYGGWVDKALQVVIEANMILPVLAVGI
                     LLYAFYQVNFWLILMIIILLNIFGSPTKTFRAAFLQVKEASYVEAARAYGATNWRIIW
                     HYLIPRITPVMVPQLIALIPSFAFLEATFAIFNVSEVMYPTWGLVIQNAMKYGAAYGS
                     QFWVLEPISLLLLTGVAFGMSSAAFDYILNPRLRTKG"
     gene            complement(4579..5172)
                     /locus_tag="PLR93_01510"
     CDS             complement(4579..5172)
                     /locus_tag="PLR93_01510"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNH25661.1"
                     /translation="MKKLILIGPVLFAVVLVTLTFVKYDFLLSLGWHPINDPTFDWPS
                     GLALGRFGWIMTATFITSGLIMTLFARRLFLDLKPGTASKLGTTLMAFAGLFLATLAF
                     TTDPTIRDTPATWHGQLHDLSFVLLGLTLFPAMVVLGFAFRTDERWKNLAVYTWGTLA
                     LAGPAFFIKGAAFYVFLFAILAWNEIAAIRLNSHAEN"
     gene            complement(5221..5619)
                     /locus_tag="PLR93_01515"
     CDS             complement(5221..5619)
                     /locus_tag="PLR93_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25662.1"
                     /translation="MQILFLIHSHMRWLILIVALVAIVKFAIGWLRGGAFKGMDRGLA
                     SGFSGLMDLQATLGLIFLIWTGLAGVGFPMNRIEHATTMIIAAVLAHLPARWKNASDT
                     VRFRNTLFCIVGSLVLVFMGVVRLRGGWTW"
     gene            5761..6534
                     /locus_tag="PLR93_01520"
     CDS             5761..6534
                     /locus_tag="PLR93_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH25663.1"
                     /translation="MTYTTILTETRGRVGIVQLNRPQAMNAFNLAMLGEVFDALEVFD
                     KDENVGAIIVTGNEKAFAAGADIKEMADQTYVQMLANEHVKIWDRIRGIRKPVIAAVS
                     GWALGGGSEFVLSCDMVIASESAKFGQPEITIGIIPGAGGTQRLARLVGKHLAMEMVI
                     NNRTLTAQEAQQFGLANRVVPVEGYLDAAVAFAEEIASRAPIAVRMAKDAVNAAFETT
                     LTEGLAVEKRNFYPLFATEDQKEGMQAFIEKRKPNWKGK"
     gene            6615..7364
                     /locus_tag="PLR93_01525"
     CDS             6615..7364
                     /locus_tag="PLR93_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNH25664.1"
                     /translation="MPASTLNLKSALSDLSLKKRLVLVHASPLVNADSLFTSLLETTS
                     GFFTPTFTPKSLLPLEYHPVHSKADVLSLAQPFTSSTPTDEAFGAFPELVRKHAKGKR
                     SIHPVFSFAGINADAILHSQTQDNLYAPIATLADEDGVILLVGMDQRVNFSIHYGEKL
                     AGRMQFIRWALTDHGVLECPNFPGDSEGFNAISLTVKPFTRQAEVDGITLQAIPVNDL
                     LKSVVNKIHEDAYSLLCGRPDCERCEAVRWG"
     gene            complement(7425..>7651)
                     /locus_tag="PLR93_01530"
     CDS             complement(7425..>7651)
                     /locus_tag="PLR93_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25665.1"
                     /translation="GLNLVRTKKVKTPLLVLGAKEDTVISNHDVEKTAQAYDTKAELF
                     PSMAHDMMLEEGWKDVANRIMEWLQEKGIE"
BASE COUNT         2035 a         1979 c         1687 g         1950 t
ORIGIN      
        1 gatgtgtatt cgctcgccgc ccaccccact tcaattcggg cgccagcatc cccgccctgc
       61 cagtcactgg cggctttgga aagcaggtcg gcggcttgcc tgcatttcgc atgttcatct
      121 ttcatatttc cgatcacgcc cgagttgacg ggaaacccgg catcttcgag agttttcata
      181 agcagttctt ccttgcgaaa gaaatttccg tctacatact catggatgaa ggtgctgcca
      241 tggatgaaaa aacccggttg aacattttta ttacctccgc caagcatggc agacccagcg
      301 ccaaatacat ccaaaaaccg ctggagagtt tcttgatctt tccgaaaatg tttcgcaata
      361 cgcatatggg tttcctgtca gtaaaacggg actttttctt tgctttaaaa ttttaccgcc
      421 ctttaataga aacaaacagc aatgaacccg tgcaattgta tgctaattta gccaactgat
      481 ctcattccaa accaagacgc aaacaacccc aaaaggacag agcgccagtc cgctggcagc
      541 cagtaagggg ctatcacgag ttttcccgat ggaaacaaaa caggtcagag ttgctctgac
      601 ctgttttact caaacgttta tttgcttttt tcttccaaca gtttcgtcaa caatcgaatc
      661 tgttcctgtt gcatttccac cagttctgcc cattgcttat cgcgcagttc atctaatttc
      721 tggtgcaatg tttcgatctc catctctgct tttaagttca cttcgtaatc atgtgctgca
      781 tccatccggt ctttcgttgc ctgccggttt tgagacatca taataatagg agcctgaatc
      841 gacgccaaca tggaaagaaa gagatttagc aaaatataag ggtatgggtc aaaagcatca
      901 ttcaacctag caagcacaac cgagttgaga aaaacccagg tcaataagat caagccaaag
      961 ataatgatga acgtccatga tccgccaaat ttggcaacat tatccgcgag gcgctgacca
     1021 aaggttagtt ggtcatcaaa ctccttgttg acgttacggg tcacgtgtct gcgatccatg
     1081 atgtggcgaa gcagctccca ttcatttctg gtaaatgttt ccattttttc taaaaacagg
     1141 tttttgaatt gttcctgaga atgattattc atggtcttta ccatccttat ctttattatt
     1201 gttacggcga tacaaccaaa tgccaattcc tatcaaagcg atcccgatca tcacgggcag
     1261 ttcatgctct ttcacatcat caaagatcaa tgtcatcaca tgcccaataa aatgcccagc
     1321 caacccaaaa gtaattgccc acagaaaagc acctataaaa tctaataaaa caaatttctt
     1381 tgtattcagt ttggacatac caagcacaaa aggtaatacg gtccttaagc catacataaa
     1441 acgcacgctg agaaaaagca gagtggaatt ccggtcaagt agagctttgg ctttttctac
     1501 atgcggctgc caggcaggtc gatttgccaa aaacgtattt ccttgaatac gccccatcca
     1561 aaagaaaaat tggtcggaag cgaaggcaac taagccaccg agcaaaataa ccaacggcaa
     1621 gtctaaatat ccgcgatgcg ccatgaaagc cgcaaggact aggactgttt caccttccag
     1681 caagagtccc aacaacaggg cgggataacc aaaatgagta atcagttgtt caagatgcat
     1741 agtcggaatt gtacgccaca taaaatcaaa atttaaaacc atactacggt ggggggagca
     1801 ttttcctagt aaatgtataa tctcaaagta gaacttgcct agaccaagat taaggaaacc
     1861 gaatgctttc tgaaaattca gagcttgccc atcagggaag tacagaggta caaagagcac
     1921 gcaaaacagc tcggagtacg ttcttcaccg ccctgaagtt catggcgaca agattgttaa
     1981 cggtccttct caccattggg ttgggcacat tcattaccat tgtggctgcc aatggtggcg
     2041 gtgccataga caacgcagaa aagagtcgca ttaatgccgt caccttgcgg agtgtggacc
     2101 ctttcgccac atctgccgaa gagcttacta aactacgagc agatatggaa gaaaaagaag
     2161 ggttaaatct cccctttctg ccgcgccaca tgtactggac gtataaagca ctgactctga
     2221 actggggaga agtaaacgac agagtgaagt ttcatccgcg ctacacaact tccaatatga
     2281 atgcagaaga tagccgcgtg atcctgatgc aataccttcc taacaccatc cttttgatcg
     2341 gtgctggctt cttctttctt tttattgcag ggattccatt tgcgctacac ctatctcaaa
     2401 accccggtgg gcggttagac aaactcatag ggacgctcac gcctctttcc tccattccgg
     2461 gctgggtcat tggtttcatt ttgattctag tcttcgctgt gggtttaaag atcctccctg
     2521 tcgggcggat gatagacagc attccacctg aatcatcctt tgaatacgtg ctcaccgtta
     2581 tcagacacat ggtcttaccg gttacagcca ttttgctttc cagcgcattc acattgatct
     2641 tttcctggcg tacctacttc ctcgcttact ctcacgaaga ttatgtagat gtaggaaaag
     2701 ccatgggtct tccgcaaaag actttccgac gaaattacat cctgcgcccg gtattgcccg
     2761 ccatcatcac cagtttctcc ctttccatgg tgggattttg gcagttgatc accaccgtag
     2821 aatttgtctt cgattggccc ggcatcggct cactattcgt acgcgccttg cctcactttt
     2881 taagtgaaag aatgtacccc ggtgaaattc ctatcatcat tggcacaatg gtcatctttg
     2941 cctacttgtt gggcttcacc gtctttatgt tggatattgc ctatctagtc acagatccac
     3001 gtatccgcgt cacatctggt ggaagctcac aaaggctggt tgccaaacgc accaagcgta
     3061 agtggacatt cccaaccttc aaacgaccag agttctcatg gacagaaaca aaacaaaatt
     3121 tctccgacac aaaagcgcaa tgggcagaga attggaagaa attccttgcg ctcttaagtg
     3181 aactacgcgg atatccctct gcgatcattg gctttagcat cgttggtctt ctggtattga
     3241 cctccatcta cactgtcatc gccttgccct atcgtcagat cggcagagat tggttcttat
     3301 taagcgtcaa accgagaagt gtggttccca aactggcttt gccaagttgg gtcaacttct
     3361 ttcgcgaaaa cgacctgcca tccactattc tcatagatag ccgcaccgat tctgtcgaac
     3421 gcacagaaac cgtactcaac gaaacgacca aaaccatcac acttacctac acctttgatt
     3481 atccttacac agaatttcca aaagatatgg tcatttatta ttacccgacc tatcaagaga
     3541 aagttccctt tgcatcgatg atcctcacca cacccgatgg gcgcgaactg gaacttcgtg
     3601 gcgagatgac gtcctttgaa aagaattacg aaccgggcag tgtgctcaat atcaataaga
     3661 ttctcaagca aaacccaaac tggaaagaat ggttctcgat cgatgagcat taccccacac
     3721 ctgctttcta tttattattt gcagacccca atgccaaaac tgccaaggca gtacccggca
     3781 catacatcct aaccatcaat gcggttggct tcgaaaaaga ctgggacctc gatgcccagt
     3841 tggtgatctt tggacaagtg tatggcatgg ctggtaccga ttacatgcgc cgtgatctga
     3901 tgactccgct tttgtggggc atgcccattg ccctcatctt tgggttgcta ggttccatcg
     3961 tcatcacggt tgggtctgct attgtaggcg cagccgccgc ttggtatggc ggctgggtag
     4021 ataaagccct gcaagtggtg atcgaagcca atatgattct gccggttctg gcagttggca
     4081 ttttgttata cgccttttac caggtcaact tttggttgat cttaatgatc atcatcttgc
     4141 taaatatttt cggcagcccc accaaaacct tccgcgctgc gtttttacaa gtcaaagaag
     4201 cttcgtacgt agaagccgcg cgtgcttatg gagcgaccaa ttggcgcatc atttggcact
     4261 acctcatccc gcgcatcaca ccggtgatgg tgccgcaact tattgctctc attcctagtt
     4321 tcgcttttct cgaagccacc ttcgccatct tcaacgtttc tgaagtaatg taccccacat
     4381 ggggtttggt cattcaaaat gcgatgaaat acggcgccgc ctacggctca caattctggg
     4441 tcttggaacc aatctctttg ctcttattga ccggtgttgc atttggaatg tctagcgcgg
     4501 catttgacta tattctcaac ccgcgcctca gaacaaaggg ataagttgat tcagtttctc
     4561 tggcttttcg ttacgctatt agttttctgc atgtgagttc aaacgaattg ccgcaatttc
     4621 attccacgcc aaaattgcga atagaaaaac atagaaagct gcgcctttga taaagaatgc
     4681 tggtcctgcc aaggcaagcg tcccccacgt atagacggca agattcttcc aacgctcatc
     4741 agtgcggaag gcgaagccga ggacaaccat cgcggggaaa agcgttagcc caagcaacac
     4801 aaacgagagg tcatgcagct gtccatgcca agtggcggga gtatcacgaa tggttggatc
     4861 ggtggtgaaa gctaaggtgg cgagaaataa accagcgaac gccatcagcg tggtgccaag
     4921 tttggaagct gtgcccggct tcaagtccag aaagaggcga cgggcgaaaa gagtcatgat
     4981 gagtccgctg gtgatgaagg tggcggtcat gatccacccg aagcgaccga gcgccagccc
     5041 actgggccag tcgaaggtgg ggtcgttgat gggatgccag cccaaactca aaagaaaatc
     5101 gtatttgacg aatgtcagcg taacaagcac aactgcaaaa agaacgggac caataagaat
     5161 taattttttc atgtttaaac aaaacagcct cgcatacgcg aggctgttcc cttttttaga
     5221 ttaccaagtc caaccgccgc gcaagcggac gacacccatg aagaccaaca ccaacgaacc
     5281 gacaatacag aacaaggtat tgcggaagcg gacggtgtct gaggcgttct tccaacgggc
     5341 gggcaaatga gcaagcactg ccgcaatgat catggtggtg gcatgttcga tgcgattcat
     5401 ggggaagcca acgcctgcca agccggtcca gatcaagaaa atcaagccga gcgtggcttg
     5461 taagtccatc agcccgctaa aaccagaagc cagaccgcgg tccatgcctt tgaaggcgcc
     5521 gccacgcagc cagccaatgg caaatttgac gatggcaacc aacgccacga tcaagatcaa
     5581 ccagcgcatg tgggaatgaa tgaggaatag aatttgcatg agagtctccg tatataaatt
     5641 tagttcagat atttgggatg aagcggatat agccgttctt acgctgccca atagggtttg
     5701 agaattatac ccagtgcagg ttgagtttat aatctcacta tctgaacttt ggaggaaccc
     5761 atgacctaca ccaccattct gaccgaaacc cgcggacgcg taggcattgt gcagttgaac
     5821 cgcccgcaag ccatgaacgc gttcaacctt gccatgcttg gcgaagtctt tgatgcattg
     5881 gaagtttttg ataaagatga aaatgttggc gcgatcatcg tcaccggcaa tgagaaagcc
     5941 ttcgctgccg gagccgatat caaagaaatg gcagatcaaa cctatgtgca aatgcttgcc
     6001 aatgaacatg taaagatttg ggatcgcatt cgcggcattc gtaaaccggt cattgcagca
     6061 gtttcgggtt gggcgctcgg cggcggaagc gaattcgtgc tttcctgcga tatggtcatc
     6121 gcgagcgagt ctgcaaagtt cggtcagcct gaaattacta ttggcattat ccccggcgca
     6181 ggcggcacac agcgactggc tcgtctcgtg ggcaaacatc tcgccatgga aatggtcatc
     6241 aacaaccgca ccctcaccgc gcaagaagct cagcaattcg gtctcgcgaa tcgtgttgtg
     6301 cctgttgaag ggtatctcga tgccgctgtt gcctttgccg aagaaattgc ttcgcgcgcc
     6361 cccatcgccg tccgcatggc gaaagatgcc gtcaatgccg catttgaaac cacgcttacc
     6421 gaaggtctag ccgtcgagaa gcgtaacttc tatccattat ttgcgaccga agatcaaaag
     6481 gaaggcatgc aagccttcat tgaaaaacgc aaaccgaatt ggaaaggtaa gtagccttgt
     6541 agttttttcg tcgagtagtc gcttgatcga tttggactac tcgacatgca accatttgat
     6601 tattcgacta acctatgccc gcctccactc ttaacctcaa atctgctctc tccgacctaa
     6661 gcctgaaaaa acgccttgtg ctggtgcacg cctccccact cgtcaatgcg gactcgttgt
     6721 ttacctccct gctggaaacc accagtggat tcttcacccc aaccttcacg cccaagtctc
     6781 tgttgccgtt agagtatcac cccgttcact ccaaagcgga cgtactttca ttagcacaac
     6841 ctttcacatc ctccacacct acagacgaag cctttggcgc ttttcctgaa ctggttcgca
     6901 aacatgccaa agggaaacgc tccattcatc ctgtcttttc ttttgcaggc attaatgcag
     6961 atgcaatcct tcattctcaa actcaggata atctttacgc acccatcgcc actttagcag
     7021 atgaagatgg agtcatcctg ctcgtcggca tggaccaacg cgtcaacttc agcattcatt
     7081 atggagagaa acttgcaggg cgcatgcaat tcatccgctg ggcgctgacc gaccatggtg
     7141 tacttgaatg cccaaacttc cccggcgatt cagaaggctt caatgccatc agtctcaccg
     7201 tcaagccctt cacccgtcaa gcagaggtgg atggcattac ccttcaagcc attccagtca
     7261 atgacctgct caagtctgtg gtcaataaaa tccacgaaga tgcctattcc ctcctatgcg
     7321 gtcgccccga ttgtgagcgc tgtgaagccg ttcggtgggg ataataaaag aattaaataa
     7381 aaagagtgga aagctaaaac ctttccactc tttttatttt cggattactc gattcctttt
     7441 tcctgcaacc attccataat cctattcgct acatccttcc agccttcttc aagcatcata
     7501 tcgtgcgcca tactgggaaa tagttccgct ttggtatcgt aggcttgggc ggtcttttct
     7561 acatcatgat tagaaattac cgtgtcctct tttgcgccaa ggacaagcaa cggtgttttc
     7621 acttttttcg tgcggaccaa gttcaacccc a
//
LOCUS       DAOQFT010000033         7469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4789_length_7469_cov_7.623280, whole genome shotgun sequence.
ACCESSION   DAOQFT010000033 DAOQFT010000000
VERSION     DAOQFT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4789_length_7469_cov_7.623280"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLR93_01535"
     CDS             complement(<1..866)
                     /locus_tag="PLR93_01535"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNH25666.1"
                     /translation="MDTISKQTYRRFLLGSQGLWPGRRFSGLSGTESALRQMQALQLD
                     PLVMVARSQDIAMYGRVLDYKPAMLYKMAYEKRKAFDYGGWLMMYPMQEFPYWQLHMQ
                     RRKEQGLRWEHFTHPPKQVVKFVLDELREKGPLGNRDIEGDAVKAWSYRGRKETSITL
                     FYLWLVGEVMISNRKGFDRLYDLRERVLPAEYDYAAPAEEAEEFFAKKHIAFSGIARE
                     PSWKTGVGDFIHRKVTAKEGAAWIERLIEKGDIARVEVEGFRENFLVLSSDQAHLSVL
                     EAGRIPKAWKHKG"
     gene            complement(948..1139)
                     /locus_tag="PLR93_01540"
     CDS             complement(948..1139)
                     /locus_tag="PLR93_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25667.1"
                     /translation="MKNTRLLLGGALIGAIIGLVAALLIGRRAEKNESETAITASEGV
                     KLGVLVFGLLRAISSLGDD"
     gene            complement(1136..1585)
                     /locus_tag="PLR93_01545"
     CDS             complement(1136..1585)
                     /locus_tag="PLR93_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25668.1"
                     /translation="MSDKLPDLAERLKSKPAHYSYMKHRNQVMRQIILPVALSALVLV
                     GIVVWVSYATFNQGGDVGRWAAISTIWVVIPMLLGGLVVLAILGGLIYGMARLLGALP
                     YYTGIAQDYIFIARGYIIRGANMVVKPINALNDWLQTAKAFFERMTP"
     gene            complement(1653..2828)
                     /gene="ftsZ"
                     /locus_tag="PLR93_01550"
     CDS             complement(1653..2828)
                     /gene="ftsZ"
                     /locus_tag="PLR93_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNH25669.1"
                     /translation="MNPTNKNVQTEAFARIKVIGVGGAGQNAVNRMMEEGIQGVEFIA
                     ANSDAQALTLSRAPIRVRLGDKITRGLGAGGDPEVGRKAAEESSDELYNVLKGADMVF
                     VTAGMGGGTGTGAAPVVSQIAKECGALTIGVVTRPFTFEGGKRSQSAESGVAKMKEHA
                     HTLISIPNDRLLQLADKKSSLQDAFRMADEVLHQGIQGISELITIPGLINLDFADVRS
                     IMSEGGAALMAVGRGSGDDRAKVAAEQAISSQLLDITIDGARGVLFNVTGGPNMTLFE
                     VNQAAAIIRETAHPDVNMIFGAVIDPNMGDEIRCTVIATGFERSGATRRVLERLPRTN
                     LSEKPVSVANTPFQTRPTESVNAPVPETRSSADVKSQPLPSINNDDLDVPTFLRNRR"
     gene            complement(2960..>3571)
                     /locus_tag="PLR93_01555"
     CDS             complement(2960..>3571)
                     /locus_tag="PLR93_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HNH25670.1"
                     /translation="TDLAIYVDGDVWHTMVLAVGGNHITQDIAHGLRLSPSQAEEVKK
                     QQGHALLSVIGPDEYFLMRPFGEDKDVRISRQDLAHIIEARVEETFNLILQEIKRSGY
                     DGLLPAGMVLTGGTSALLGINRVASEVLDMPVRVAQPENLKGLVDKLNSPAYSTSFGL
                     LQWAISMHDHQVTVSGGAKRRKPRGEGNVKFDAIKDWMKRLLP"
     assembly_gap    3572..3671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3672..3862)
                     /locus_tag="PLR93_01560"
     CDS             complement(<3672..3862)
                     /locus_tag="PLR93_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HNH25671.1"
                     /translation="MEEIVVGIDVGTTKICTLVGRVEDDKSIRILGVGIEPSEGIRKG
                     IVVDLAAASRAIKRSVEKA"
     gene            complement(3864..4931)
                     /locus_tag="PLR93_01565"
     CDS             complement(3864..4931)
                     /locus_tag="PLR93_01565"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNH25672.1"
                     /translation="MSEKRPLTRAEQVRQRRTERAAKELQQAAKQATKPIVKVSSRTP
                     TIPIMAAPKKQQDRRYNIALGMPEFHLKKPKFSWPQFSAPRMPRMPQFSANWRLGALI
                     LSVVFVVALVLAFQLPYFYVPQAFVSGNARIPSEEINGIMGVSNQNIFTVQPDELERR
                     LRLNYPEITSAQVDVYLPNYVYVTVTERQPVVMWRQNGGFTWIDATGVAFRPRGEAAA
                     LAVVDAVDAPPAGVQTSADPYSPPPFIQKEYVDAALALAPLVPAGSTLTYSAADGFSW
                     KDPRGWTVAFGTTANDVPLKIRVYQSLVDSLTQKGKIPEFISVVYPDGPYYRMKKAVT
                     VEEDQTAQTDQSTEETVVENP"
     gene            complement(4928..6046)
                     /locus_tag="PLR93_01570"
     CDS             complement(4928..6046)
                     /locus_tag="PLR93_01570"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNH25673.1"
                     /translation="MTKKLRVAVLFGGRSGEHDVSLMSARSVVSVLDPNRYDVIQVGI
                     TLDGNWLTGENALDAFEKGDTSDLDRVIPPTDPASRQSLHVLRDTKMGEKFETLAAID
                     VFFPVLHGPFGEDGTIQGLFEMADVAYVGAGVAGSSVGMDKGIFKDVMRANGIPQVPS
                     MLVLRGDIESKMDDVIKRAEELGDYPLFTKPANLGSSVGISKCNNRSDLAEGLMDAAR
                     YDRRIVIESGVKEVREVEVSVLGNEEPQASVCGEILPSREFYSYESKYIDGTSGLLIP
                     SPLPEEISNKIREYAIRAYKAIDCAGMARADFFVEKETNKIYLNELNTLPGFTSISMY
                     PKLWQASGLTYAQLVDRLIELALDRKAQRDRTEHRRTA"
     gene            complement(6049..6969)
                     /gene="murB"
                     /locus_tag="PLR93_01575"
     CDS             complement(6049..6969)
                     /gene="murB"
                     /locus_tag="PLR93_01575"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNH25674.1"
                     /translation="MDTTFAVDLLRQKFGDAVQENVTLAPYTSARIGGPADIMLSVKS
                     ADELAEAMQLIWEHNFPYYILGGGSNVLVSDQGFPGVVVLNRAKETRFETGDQPTVWC
                     ESGVIIANLAKRCASKGLAGLEWSATVPGTVGGAVYGNAGAFGGDMTHNLIWAELLTK
                     NGREKFGLEQMGYGYRTSVLKRGEAQGIVLSALLKLKNSSKEEVSVKIEQFSERRKAT
                     QPPGASMGSMFKNPEGDYAGRLIESAGLKGTRIGNAEISTVHGNFFINHGETKAEDIL
                     ALIHVVQNTVKEKFNIQLELEVELVGDWNK"
     gene            complement(6984..>7469)
                     /locus_tag="PLR93_01580"
     CDS             complement(6984..>7469)
                     /locus_tag="PLR93_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNH25675.1"
                     /translation="VMGLPMQKAAEALSQFTGTGRRFEVRGEPKGVLLVDDYAHHPTE
                     IRATLAGARARYPERRIWAVWQPHTYSRTQTLQYEFSRAFNDANEVLVTEIYASREAK
                     QEFSSAEVVASMPHSSARYSGSIEETKKYLLEHVRFNDVVIVLSAGDADQITAALLKE
                     L"
BASE COUNT         1838 a         1956 c         1785 g         1790 t
ORIGIN      
        1 cccttatgct tccacgcctt ggggatgcgc cccgcctcaa gaacagacaa gtgggcttgg
       61 tcgctgctca acacaaggaa gttttctctg aagccttcta cttctactcg agctatgtcg
      121 cctttttcaa tgaggcgttc gatccatgcg gcaccctctt ttgcagtgac tttacgatgg
      181 atgaaatcac cgacgccggt cttccatgaa ggttcgcgcg caatacctga aaaagcaata
      241 tgctttttcg caaagaattc ttcggcttct tcagcaggag cggcataatc atattccgct
      301 ggcaagacgc gttcgcgcag atcgtagaga cggtcaaagc ctttgcggtt agaaatcatc
      361 acctcaccca ccagccaaag gtaaaacaag gtgatggatg tttccttacg cccacggtag
      421 ctccatgctt taacggcatc gccttcaata tcacgattcc caagcggacc cttttcgcgc
      481 agctcgtcca acacgaactt aacgacttgt ttaggcgggt gcgtgaaatg ctcccagcgc
      541 aaaccctgtt ctttacgtcg ctgcatgtgc agttgccagt atggaaactc ctgcatgggg
      601 tacatcatca accagccgcc gtaatcgaag gcttttcgtt tttcatatgc cattttgtat
      661 agcatagcgg gtttgtaatc gaggacacgt ccatacatgg ctatatcctg actgcgagca
      721 accatcacaa gtgggtcaag ctgcaacgcc tgcatctggc gcaaagcgga ttcggtgcca
      781 gaaaggcccg agaaacggcg cccaggccaa agtccctgtg agccgagaag aaagcggcgg
      841 tatgtttgct tggagatggt atccatggtt aagggtttac attcaaaggt caagataaaa
      901 aagaccgagg caggaacctc ggtctgtggt caatcgttta tcgtctgtta atcgtcgcca
      961 agggaagaga ttgcgcgaag caagccaaat accagcacgc ccaatttgac gccttcactt
     1021 gcagtgatgg cagtttcgct ctcgttcttt tcggcgcggc gaccgatgag caaagcggca
     1081 accagcccga taatagcgcc gatgagtgcg ccgcctaaaa gtagtcttgt atttttcatg
     1141 gagtcatcct ttcaaagaag gcttttgcag tttgaagcca atcgttcaga gcgttaatgg
     1201 gtttgacgac catattcgct ccgcgaatga tatagccgcg tgcgataaaa atataatctt
     1261 gggcaatgcc agtgtaatat ggcaacgctc caagtaaacg agccatgcca tagatcaagc
     1321 cgccaaggat ggcaagtacc accaacccgc cgagcaacat ggggatcaca acccaaattg
     1381 tggagatggc agcccagcgc ccgacatcac caccttggtt gaaggtggca taactcaccc
     1441 acactacaat tccaaccaat accaaggcac tcaatgccac tggcaagatg atttgcctca
     1501 ttacctgatt acggtgcttc atgtaagagt aatgggcggg tttgcttttt agacgttcag
     1561 cgagatcggg gagtttatcg ctcataaaga tattttacca aaataaaaga ccctaaaggt
     1621 ttgaaccttt agggtctttg cttgtttagt gattatcggc gatttctcaa gaaagtcggg
     1681 acgtcaagat cgtcgttgtt gatgcttggc aaaggctgag acttcacgtc tgctgaggaa
     1741 cgggtttcag gaactggagc atttaccgat tcagtggggc gagtctggaa aggagtgttc
     1801 gccacagaga cgggtttctc gctcaggttt gtgcggggca agcgctcgag aacgcgacga
     1861 gtagcgccgc tgcgttcgaa gccggtggca atcactgtac agcggatttc gtcgcccatg
     1921 ttcgggtcga tcactgcacc gaagatcatg ttcacatcgg ggtgagccgt ttcgcggatg
     1981 attgcagccg cttggttgac ttcgaagagc gtcatgttcg gtccgccggt gacgttgaag
     2041 agcacgccac gagcaccgtc gatggtgata tcgagcaatt ggctggagat ggcttgttca
     2101 gcagccacct tggcgcggtc atcgccagag ccgcgaccta ctgccataag agccgcgccg
     2161 ccttcagaca taatcgagcg cacatcggca aagtcgaggt tgatcaagcc agggatcgtg
     2221 atcaactcgg agataccttg aataccctga tgcaacactt cgtccgccat gcggaaggca
     2281 tcttgcaggg atgatttctt atcagccagt tggagcaaac gatcattcgg aatggaaatg
     2341 agcgtgtgcg catgttcttt catcttcgca acaccagact cagcggactg tgaacgtttg
     2401 ccaccttcaa aggtgaaggg acgagtcaca acgccaatgg tgagagcgcc gcattctttg
     2461 gcgatctgtg aaacgacagg cgccgcaccg gttccggtac cgccgcccat gcctgcagtg
     2521 acgaagacca tgtctgcgcc tttgagcaca ttgtagagtt cgtctgagga ttcttctgca
     2581 gctttgcgac cgacctcagg gtcaccgcct gcgccgagac cacgcgtgat tttatcgccg
     2641 aggcgcacac gaatgggagc acgtgacaga gtcaaggctt gggcatcaga gttggcagca
     2701 ataaattcaa cgccctgaat accttcctcc atcatgcgat taacagcatt ttgaccggca
     2761 ccaccaacac caatcacttt gatgcgggcg aaagcttcgg tctgtacatt tttattagtc
     2821 ggattcattt gttcctcctg gcaaggaata tgcctaatct taaacgagaa tacgtttttt
     2881 taacagggtt aatttcgacc aaaattcaca ctttgtgaat cttttctcat gatgtcaagg
     2941 gttaaacaga atctcgtcat catggtaaca acctcttcat ccaatcttta atcgcgtcaa
     3001 atttgacgtt tccttcaccg cgcggcttgc gacgtttagc gccgccagac acagtcactt
     3061 gatggtcgtg catgctgatc gcccattgca acaacccaaa acttgttgaa taagctggtg
     3121 aattcaattt atcgaccaac ccttttaagt tctcaggttg agccacacga acgggcatat
     3181 ccaatacttc acttgcaact cgattaatgc ccaataaagc agaagtaccg ccagtcaaca
     3241 ccatgccagc agggagcaga ccatcatacc cagagcgctt gatctcttgc aagatgaggt
     3301 taaaggtttc ttccacacgc gcttcgatga tatgggctag atcttggcgg ctaatgcgaa
     3361 catctttatc ttcaccgaag ggacgcatca ggaagtattc atcgggtccg atcacagata
     3421 gcagggcgtg cccttgctgt tttttcactt cctctgcctg agaaggtgac aagcgtaatc
     3481 cgtgcgcaat atcttgagtg atgtgattgc cacccactgc aagaaccatg gtatgccaca
     3541 catcgccatc gacatagatc gcaaggtctg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn ntccgctttt tcaaccgaac gcttgatggc gcgtgaagcc gccgcaaggt
     3721 ctacaacaat ccctttgcgg attccttcag acggctcgat tccaaccccc aagatacgaa
     3781 tggacttatc gtcctccact cgcccaacga gggtgcagat tttggtcgtg cctacatcaa
     3841 tgcctactac gatctcttcc atgttacggg ttctctacta cggtttcttc agtggattgg
     3901 tcagtttgag ccgtctggtc ttcttcaaca gttacggctt tcttcatacg atagtaagga
     3961 ccatcgggat acacaacact aataaattca gggattttgc ctttttgagt caacgagtca
     4021 accagagatt gataaacacg aatcttcaac ggcacatcat tcgccgtggt gccaaaggca
     4081 acggtccagc cacgtgggtc tttccagcta aagccatcgg cggcggaata ggttagggtg
     4141 gagccagcag gcacgagcgg agcaagagcg agagcggcat ccacatattc tttttggatg
     4201 aaaggcggcg ggctataggg atccgccgat gtttgaacgc cagcaggcgg tgcatcaaca
     4261 gcgtccacaa ctgccagagc cgccgcttcg cctcgtggtc taaaggcgac gcctgttgca
     4321 tcgatccagg taaagccgcc attctgacgc cacatgacca caggctgacg ttctgttact
     4381 gtgacataga catagttggg aaggtaaaca tccacttgtg cagaagtgat ctcggggtaa
     4441 tttaagcgca agcgacgttc gagttcatcg ggctgaaccg tgaaaatgtt ttgattcgaa
     4501 acgcccataa tgccatttat ctcttcgctt ggaatacgag catttccaga gacaaacgcc
     4561 tgagggacat agaagtatgg gagttgaaaa gcgagcacca aagcaaccac gaacaccact
     4621 gaaagaatca acgcaccgag gcgccaattt gcagagaact gcggcatacg tggcatgcgc
     4681 ggagcagaaa actgcggcca ggaaaacttg ggtttcttga gatggaactc tggcatgccc
     4741 aaggcaatgt tgtaacggcg gtcttgttgt ttcttcggcg ctgccataat cggaatagtg
     4801 ggtgtgcgcg aagatacttt gacgatcggt ttggttgcct gtttggctgc ttgttgcagt
     4861 tcttttgcag cgcgttcggt gcggcgctgg cggacttgtt ccgcacgagt gaggggtcgt
     4921 ttttcgctca tgctgtcctc cggtgttcgg tacgatcacg ctgtgctttg cggtcaagcg
     4981 caagctcgat caggcggtcg accagttgag cgtaggtcaa gccgctcgcc tgccaaagct
     5041 tggggtacat actaatggaa gtaaaaccag gaagcgtgtt caattcgttt aggtaaattt
     5101 tattggtttc tttttcaaca aagaaatcgg cgcgtgccat gcctgcacaa tcaatagctt
     5161 tgtaggcacg aatggcatac tcacgaatct tgttggaaat ttcttcaggt aacggtgaag
     5221 ggattagcaa gccagaggtt ccgtcaatgt atttcgattc ataggaataa aactcgcggc
     5281 tcggaaggat ctcaccacaa acagaagctt ggggttcttc attgcccaac acactcactt
     5341 caacctcgcg cacttctttc acgccgcttt caatcacaat acggcgatcg taacgagctg
     5401 cgtccatcaa accttcagcg aggtctgaac ggttattgca tttgctgata cctacagagg
     5461 aaccgagatt ggcaggcttg gtaaacaagg gataatcgcc caattcttcg gctcttttga
     5521 tgacatcatc catcttactt tcaatatcac cgcgcagcac caacatggat ggcacctgcg
     5581 gaataccatt ggcacgcatc acatctttga agatgccttt atccatgcct acagaagagc
     5641 ccgcaacccc tgcgcccaca taagcaacat ctgccatttc gaacagccct tgaatggtgc
     5701 catcctcgcc gaagggtccg tgcagaacgg ggaagaacac atcaatcgcg gcaagagttt
     5761 caaacttctc gcccattttt gtatcacgca agacatgtaa actctggcgt gaagccgggt
     5821 ccgtaggagg gatgactcga tctaaatcgc tggtatcgcc cttttcaaaa gcgtctaatg
     5881 cattttcacc ggtcaaccaa ttgccatcca gagtaatgcc tacttgaatc acgtcatagc
     5941 gattcgggtc taacactgaa acgaccgagc gcgcagacat cagcgaaaca tcatgttcgc
     6001 ctgaacgtcc accaaataaa actgccactc gaagtttctt cgtcatcgtt atttgttcca
     6061 atcgcctaca agttcaactt ccaattccaa ctgtatgttg aatttctctt ttaccgtgtt
     6121 ctgaactaca tgaatcaaag ccagaatatc ttctgctttg gtttcaccgt gattgatgaa
     6181 aaaatttccg tgcaccgtgc tgatctcagc gttgccgatg cgcgtcccct tcaagcctgc
     6241 agattcaatc aatcgcccgg catagtcacc ttcggggttc ttgaacattg aacccatgct
     6301 ggcgcccggc ggctgtgtgg cttttcggcg ttcactgaat tgctcgattt taacagacac
     6361 ttcctctttc gaagaatttt ttaacttcaa taatgcactt aacacaatcc cttgcgcctc
     6421 gccgcgcttc aaaacactgg tgcgatatcc atagcccatt tgttcaagcc cgaacttctc
     6481 acgtccattc ttggtgagca actctgccca aataaggtta tgggtcatat cgccgccaaa
     6541 tgcccccgca ttgccataaa ccgctccgcc cactgtgcca gggactgtgg ctgaccattc
     6601 gagcccggca agacccttgg atgcgcagcg cttggcaaga tttgcaatga taactcccga
     6661 ttcacaccag accgtgggct ggtcgccagt ttcaaaacgg gtttccttcg cccgattcaa
     6721 aaccactacc ccaggaaagc cttgatcgct caccaacaca ttcgaaccgc cgccaagaat
     6781 gtaataggga aagttatgtt cccagatcaa ctgcatcgcc tctgccaact catccgcaga
     6841 tttgaccgaa agcataatgt cagcgggtcc accaatgcgc gcagacgtgt acggcgcgag
     6901 agtcacattc tcttgcaccg catcgccaaa cttctggcgc aacaagtcaa cagcaaaggt
     6961 ggtatccacc atcaacatcc taactagagt tccttcaaca gcgccgccgt gatctggtct
     7021 gcatcgcccg cagagagcac aatgaccaca tcattaaagc gcacatgttc caataaatat
     7081 tttttcgtct cttcaataga accgctatac cgagccgaag aatgcggcat ggaagcgact
     7141 acttctgcag aagaaaattc ctgtttggct tcgcgtgaag cgtagatttc cgtcaccaac
     7201 acttcattgg catcgttgaa tgcgcgtgaa aattcatatt gcaacgtttg agtccgtgaa
     7261 taagtgtgtg gctgccacac cgcccaaatt cggcgttcgg gataccgagc gcgagcccct
     7321 gctaacgtag cacggatctc agtgggatga tgggcataat catcaacaag caacacaccc
     7381 ttcggctccc cccggacctc aaagcggcgc cccgtacctg tgaattggct caacgcctca
     7441 gccgcttttt gcataggaag ccccatcac
//
LOCUS       DAOQFT010000034         7451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4810_length_7451_cov_5.985938, whole genome shotgun sequence.
ACCESSION   DAOQFT010000034 DAOQFT010000000
VERSION     DAOQFT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4810_length_7451_cov_5.985938"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLR93_01585"
     CDS             complement(<1..684)
                     /locus_tag="PLR93_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNH25676.1"
                     /translation="MSRTIHVLFLAAEADPFIKVGGLGDVSGVLPRELRALSDDELKL
                     DVRLVLPYHPAVKAENLRPIEIFSIPRGDSEVTVEAFETTFNGMPVYFIGGEPIHSSG
                     SVYSLDAKLDAEKYAFFSLAAMELPKHINWQPDVIHANDWHTALSLYVNLTKRWEAGA
                     KHIVNVLTLHNMPFMGADVRAILEQYQIQLAQTDLPEWARVLPLPLGLWASDAIVAVS
                     PGYAGEVLTE"
     gene            complement(712..1515)
                     /locus_tag="PLR93_01590"
     CDS             complement(712..1515)
                     /locus_tag="PLR93_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNH25677.1"
                     /translation="MKHHLVEPGKKIKLAEVDPQDTGKFKGGKEEGLKEIEKLNEKLQ
                     ELQELLYAEGKHKVLVVLQAMDTGGKDGAIRRVFDGVNPQGVKVASFKVPTSEEMAHD
                     YLWRVHKVVPASGELVVFNRSHYEDVLVVRVHNYVPKEVWSKRYEQINAFEKLLAENG
                     TTILKFFLHISKDEQKERLQARLDDPSKHWKFSVGDLGERKLWDEYQAAYEDALNKTS
                     TEHAPWYVVPANRKWYRDLVISTVLVDTLKGLKMNYPEPKDDLSGVVIK"
     gene            complement(1599..1673)
                     /locus_tag="PLR93_01595"
     tRNA            complement(1599..1673)
                     /locus_tag="PLR93_01595"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1639..1641),aa:His,seq:gtg)
     gene            complement(1704..2171)
                     /locus_tag="PLR93_01600"
     CDS             complement(1704..2171)
                     /locus_tag="PLR93_01600"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25678.1"
                     /translation="MPEMRPVTKDNWKELVALKVRDDQKHFVASNLYSIAESQFGETI
                     PGEGHWDMFPYGMYDEGTPVGFLMLSYNYEFARFQGFILRLMVDDAHQGKGYGKFGMN
                     WMLEKYRADGRVRVVGISYEPNNEVARTLYANLGFQETGEIVDGEALALLNLR"
     gene            complement(2164..3441)
                     /gene="hisS"
                     /locus_tag="PLR93_01605"
     CDS             complement(2164..3441)
                     /gene="hisS"
                     /locus_tag="PLR93_01605"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNH25679.1"
                     /translation="MANKIQAVKGTREFYPEQMALRNFIYSKVRSAAHAFGYQEWDAP
                     FIETIDLYAAKSGEELVKKQSFTFEDRGGDFVTLRPELTPSLARMIAAKQGELTFPVR
                     WWSFGPFWRYEQPQKGRSREFFQWNVDMLGVNSPEADAELIAVGATFLRSVGLSPERA
                     LIYVNNRRLMDSEFDALGISAEKRLDVSNLVDRRTKMEPAKWDAYALDLGLNQTQLEG
                     LKSILGNFELWKKSDELVRLFATLEALGVKEYVKFDPNIMRGLLYYTGTVFEAFDVTG
                     SLKRAIFGGGRYDNLLADVGGQPLSGVGFAMGDVVIGIILQEAGLLPEFIPSPAQVLV
                     TVFDEKLWMQSFALAAELRAAGLNVMVSPEPTKLPKQFKFADKMKMKIALVIGPDEAA
                     QGLVVVKNLVNGEQVQVKKEAVLDSIQSVLKHA"
     gene            3564..>3889
                     /locus_tag="PLR93_01610"
     CDS             3564..>3889
                     /locus_tag="PLR93_01610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH25680.1"
                     /translation="MKIDYQEPSKDLLMRIDIHEKYGSANIDVWTNDLLKPQAGMSIL
                     DVGCGAGKLSFLFDDYTKRQAKITGGDFSEELLDKARAKNKERGSNIDFQFLDFNEKF
                     KFEDNT"
     assembly_gap    3890..3989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3990..4650
                     /locus_tag="PLR93_01615"
     CDS             <3990..4650
                     /locus_tag="PLR93_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNH25681.1"
                     /translation="RVAPTGFNMNFFGKRVLFIGAHPDDIELGCGALLHHIVKNTDAS
                     VLCVTLSDNQKNPDLKKVKNEHLNAMQVLGVPSDNVVLGPFTTRVFPDSRQEILEYFL
                     KLRKEFKPDLIFTHSKQDVHQDHNTMTDEALRAFRGITVLGFDVVRSSYGFFPHFLVE
                     VTEEDVNKKIEALSQYDTYQDRYYFNAELTRSIMVRHGALAERPFAEGFDILRIVGKF
                     E"
     gene            complement(4685..5566)
                     /locus_tag="PLR93_01620"
     CDS             complement(4685..5566)
                     /locus_tag="PLR93_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25682.1"
                     /translation="MSSLSNIAIKLIPASHFSIDELTGIYNQTRVDYMVPMPMNSARL
                     MEYIEFYDVDLDRSVVAIEDGELRGVAMLGVRAERTWVTRLGVIPSTRRGGVGESLMQ
                     SLLEQSARLGIDFTMLEVIKNNVPAHRLFLKLGFYEIGELLILRRAPSKKLVEPVIAD
                     ARRLERYEALDLVGYDRGTQPWTNQSESMSHAQDISGLYLTLQDGSQGWLVYQRQKFT
                     LTHFIYKTEVGDPATMAYAFLSHLHHQYPRLDTHIENIQVNDPHLPGFQKMGYVESFR
                     RIEMWRGNPPSSIMPSK"
     gene            complement(5576..5947)
                     /locus_tag="PLR93_01625"
     CDS             complement(5576..5947)
                     /locus_tag="PLR93_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25683.1"
                     /translation="MTRILIVEDVPDNVELARKILTSRGYEVIHAINAEMGYNLAIQE
                     RPDLVLLDLGLPDYDGLTLAGQIRENPAFERLPIIAFTAWPQETAKNMAESYGCNGFI
                     SKPIIRVNEFIAQVESFLLND"
     gene            6125..7258
                     /gene="sucC"
                     /locus_tag="PLR93_01630"
     CDS             6125..7258
                     /gene="sucC"
                     /locus_tag="PLR93_01630"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNH25684.1"
                     /translation="MKLHEYQSKNIFSKFGIPIPKGRVATTAQEAKQIAEELGGRVVI
                     KSQVLVGGRGKAGGVKLAKDPAEAEQLATQILGMEIKGLPVRKVLVDEASAIDQEIYF
                     AITDDRAAKKPVLIASAAGGVDIEEVAAKTPEKIIKVNIDPLLGLREYQARDVAVAID
                     LPRDYWKDFTKIAMGLWHVYQSTDASLAEINPLVITKDKKMIALDGKMMIDDNALFRQ
                     SDLAEIRDTDEDAPAEIEARKYGLSFIKLDGNIGCMVNGAGLAMTSMDVIKLFGGDPA
                     NFLDVGGGAGAEKVAAAMRIILTDPNVKAVLFNIFGGITRCDEVAKGILVAMDEVKPK
                     VPMVVRLVGTNAEEGRRLLENANMITAETLADAAKKAVAAAKG"
     gene            7264..>7451
                     /locus_tag="PLR93_01635"
     CDS             7264..>7451
                     /locus_tag="PLR93_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNH25685.1"
                     /translation="MSILVDKNTRLLVQGITGNEGLFHTTQMAAYGTNIVGGITPGKG
                     GEWVLDGKIPVFDSVKSA"
BASE COUNT         2004 a         1913 c         1576 g         1858 t
ORIGIN      
        1 ttctgtgagc acttcccccg cataccccgg tgagacggca acaatcgcat ccgatgccca
       61 aagccctaag ggcaagggca aaacacgcgc ccattcaggc aaatcggttt gagccaattg
      121 aatttgatac tgctcaagaa tagcgcggac atccgccccc atgaaaggca tattatgcag
      181 ggtaagtaca ttgacaatat gttttgcacc ggcttcccat cgtttagtca aattgacata
      241 taaactcagt gcggtatgcc agtcgttggc atgaataaca tctggttgcc agttgatgtg
      301 cttggggagt tccatcgccg ccagtgagaa gaaagcatac ttctcagcat ccaattttgc
      361 gtctaaggaa tacaccgagc cgctggaatg gatcggttca ccgccaataa aatagaccgg
      421 cattccatta aatgtagttt caaacgcttc gacagtaact tcagaatccc cgcgcggaat
      481 ggaaaaaatt tcgatagggc gcaaattttc cgctttcacc gctgggtgat acggcaaaac
      541 caacctcaca tctaatttca attcgtcatc tgaaagagcg cgcaactctc gcggcagcac
      601 ccctgaaaca tcgcccagcc cgccaacttt gataaaaggg tcggcttcgg cagcgagaaa
      661 aagcacatgg attgttctag acatgagcgc tattttacac cctttgcaga cttacttgat
      721 cacaaccccg ctcaaatcat ccttcggctc ggggtaattc atctttaagc ctttgagcgt
      781 atccaccaac actgtggaaa tcaccaaatc gcgataccat ttgcggttgg caggcaccac
      841 ataccacggg gcatgttcgg tgctggtctt attcaatgca tcttcataag ccgcctgata
      901 ctcatcccac aatttgcgct cgccaaggtc accaacgctg aacttccaat gcttggatgg
      961 gtcatctaag cgtgcttgca ggcgttcttt ctgctcatct ttactaatat gaaggaagaa
     1021 cttcaggata gtcgtgccgt tttctgccag aagcttctca aaagcgttga tctgctcata
     1081 acgcttcgac caaacttcct ttggcacgta gttatgtaca cgcaccacta aaacatcttc
     1141 gtaatggctg cggttgaaca ccaccaattc tccactggca ggcactactt tatgcacacg
     1201 ccataaataa tcatgcgcca tttcttctga ggtcggcact ttaaagcttg ccaccttcac
     1261 cccctgcggg ttgacgccgt caaatacccg ccgaatggca ccatctttcc caccggtgtc
     1321 cattgcctgt aacaccacca agaccttatg cttcccttca gcataaagta actcctgcaa
     1381 ttcttgcagt ttttcattta atttctcaat ttctttaagt ccttcttcct taccgccctt
     1441 aaacttcccc gtatcctgcg ggtctacctc tgctaatttg atctttttac ccggttcaac
     1501 caaatgatgt ttcatgagac ctccgatatt aaaaccttta ttgcacaggt atatatcata
     1561 ttaaaacaaa aagacctaga ttaaggacca ggtctttttg gggcggatga tgggtttcga
     1621 acccacaacc ctcacagcca cagtgtgatg ctctgccatt gagctacatc cgccatataa
     1681 agcggacaaa attttaccac aattcagcgc aggttcaaga gtgccaacgc ctcaccatct
     1741 acgatctcgc cagtctcttg aaaccctaag tttgcatata atgtacgcgc cacttcattg
     1801 ttcggctcat aactaatccc cacaacccgc acacgcccgt ccgcgcgata tttttcgagc
     1861 atccagttca taccgaattt gccatacccc ttgccctgat gcgcatcgtc caccatcaac
     1921 cgcaggataa aaccctgaaa tctcgcaaac tcatagttgt agctgagcat caaaaaacca
     1981 actggcgttc cttcatcgta catcccatat ggaaacatat cccagtgccc ctcgccagga
     2041 atcgtctcgc caaattgaga ttcagcaatg gaatacaaat ttgacgccac aaaatgtttt
     2101 tggtcatccc gaactttcaa ggcgaccaat tctttccaat tgtcttttgt gacagggcgc
     2161 atctcaggca tgtttcagaa cgctttggat cgaatcaaga accgcttctt tcttgacctg
     2221 aacctgctcg ccattcacaa gatttttgac gaccaccaag ccttgcgccg cctcatctgg
     2281 accaataacc agcgcaattt tcatcttcat cttgtctgca aacttaaact gcttgggcag
     2341 ttttgtcggc tctggcgaga ccataacatt gagtcccgcc gcacgtaatt ctgccgcaag
     2401 cgcaaaagac tgcatccaca atttctcgtc aaagaccgtt accaacacct gtgccgggct
     2461 gggaatgaat tcaggcagca aaccagcctc ttgcaaaatg atgccaatga ccacatcgcc
     2521 catggcaaag cctacgccag aaagaggttg cccacccaca tcggcaagca gattgtcgta
     2581 gcgcccgccg ccaaagatgg cgcgtttgag tgaaccggtc acgtcgaagg cttcgaagac
     2641 ggtgccggtg taatagagaa gtccacgcat gatgttcggg tcaaatttga catattcttt
     2701 gacacccaat gcttccagtg tcgcaaagag acggaccaac tcatcactct tcttccacag
     2761 ctcaaaatta ccaagaatag acttcaagcc ttctagttga gtttgattca agccaaggtc
     2821 aagggcgtac gcatcccatt tggcgggttc catctttgtg cggcgatcca ccaaattaga
     2881 aacatcgagg cgtttctcgg cagaaatgcc taaagcgtcg aattcagaat ccatcaggcg
     2941 gcgattattc acatagatca gagcccgctc cgggctgagt ccgaccgagc gcaggaacgt
     3001 tgctccgacg gcgatcagct ctgcatccgc ttctggcgag ttgactccca acatatccac
     3061 attccattga aagaactcac gtgagcgccc cttttgtggc tgctcatacc gccaaaacgg
     3121 accgaacgac caccaacgca ccgggaaagt taactcccct tgttttgccg caatcatacg
     3181 tgccagcgac ggtgtaagtt cggggcgcaa tgtcacaaaa tcgccaccac ggtcttcaaa
     3241 cgtgaaagac tgcttcttaa ccaactcctc gcccgatttc gccgcataca gatcaatcgt
     3301 ctcgataaat ggcgcatccc attcctgata accaaaagca tgcgcagcag aacgaacctt
     3361 actgtagata aagtttcgta acgccatctg ctctgggtaa aattctcttg taccttttac
     3421 tgcctgaatc ttgtttgcca tgtatactcc aaatgccacg cattttaaca tgactcattc
     3481 caggattaat cgtaaataag catttcaaaa ttcacccact gaattttggt ataatcccaa
     3541 catcaacttc aaggagagat gatatgaaga tcgactatca ggaacccagc aaagaccttt
     3601 taatgcgaat tgatattcat gaaaaatatg gctctgccaa catcgatgtt tggaccaacg
     3661 acctgctcaa acctcaagca ggcatgagca ttctcgatgt aggttgcggt gccggaaagc
     3721 tcagcttttt gttcgacgat tacaccaagc gccaggcgaa gatcaccggc ggtgacttct
     3781 ctgaagaact gctcgacaaa gcgcgcgcca aaaataaaga acgcggctcg aacattgatt
     3841 ttcaatttct agactttaac gaaaagttca agtttgaaga taataccttn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgagtcgccc ctacgggttt caatatgaac
     4021 ttcttcggca aacgagtcct ctttattggc gcgcacccag acgacatcga attgggatgt
     4081 ggggcgcttt tgcatcatat cgttaagaat acagatgcaa gtgttttatg cgtcaccctt
     4141 tctgataatc aaaaaaaccc tgacctaaaa aaggtcaaga atgaacatct taacgcaatg
     4201 caagtactgg gtgtgccttc agataatgta gtattaggac ccttcaccac acgcgtcttt
     4261 cccgattcgc ggcaggaaat tcttgaatat tttctaaaac tgcgcaaaga attcaagcct
     4321 gatctaattt ttacccattc caaacaagat gtacaccaag atcacaacac aatgactgat
     4381 gaagccttgc gcgcctttcg aggtattact gtattgggtt ttgatgtggt tcgttcatct
     4441 tatggtttct tcccgcactt tctcgtggaa gtcactgaag aagatgtaaa caaaaaaatc
     4501 gaagccttat cccaatacga tacctaccaa gaccgctatt acttcaacgc cgagctcacc
     4561 cgctctatca tggtccgcca tggggcgttg gcggagcgtc cgtttgcgga ggggtttgat
     4621 attttgagga ttgtagggaa gttcgaataa tataaaaaat aaaacaggct ttcagaagcc
     4681 tgttttattt tgaaggcata atcgatgacg gaggattacc ccgccacatt tcgatgcggc
     4741 gaaaggattc aacatatccc attttctgaa agcccggtaa atgtgggtcg ttgacttgaa
     4801 tattttcaat atgcgtatcc aaccgtggat attgatgatg cagatgcgat aaaaaggcgt
     4861 atgccattgt agctgggtca cccacttcgg tcttgtaaat aaaatgcgtc agcgtaaatt
     4921 tctgccgctg gtacacgagc cagccttggc tcccgtcttg aagcgtgagg tagagaccgg
     4981 aaatatcctg cgcatggctc atcgattcgg attgattcgt ccatggttga gtcccacggt
     5041 catatcccac aagatccagt gcttcatatc gttccaatcg acgtgcatct gctattacag
     5101 gttccactag ttttttagat ggcgcacgcc taagaatcag caactcccca atttcataaa
     5161 acccaagttt caggaacaat cgatgggcag gaacgttatt tttaataact tccagcatgg
     5221 tgaagtctat gcccagtctg gcagattgct ccagcaagct ttgcatcaag gattcaccaa
     5281 cgccaccccg ccgcgtggac gggataacac ctaaacgggt gacccatgtc cgctcggcac
     5341 gaacccccaa catagctaca ccccgtaatt ctccgtcttc aattgcaaca accgaacgat
     5401 ctaaatctac atcataaaac tcaatatatt ccatgagacg cgcagagttc atgggcatgg
     5461 gaaccatata atctacacga gtttggttgt agatgccagt caattcgtca attgaaaagt
     5521 gactagcggg gattaactta atggcaatat ttgataaaga agacattctt aagggttagt
     5581 cgtttaagag gaaactctct acttgagcaa taaactcatt cacacgaata ataggtttac
     5641 taataaaacc attgcagcca tagctttctg ccatgttttt tgctgtctct tggggccatg
     5701 cagtaaaggc aatgattggc agtcgttcaa aggctgggtt ttcacgaatt tgcccagcaa
     5761 gggtcaaacc gtcataatcg ggcaacccca aatccagcaa tacaaggtct gggcgttctt
     5821 gtatggcaag gttgtatccc atttcagcat ttatggcatg aataacttca taccctctcg
     5881 aagtaaggat ctttcgagct aattccacat tatcgggcac atcttctaca attaggattc
     5941 gcgtcatagt tacctttggt aaaacttaag gtcaaagtaa tgttaccact tgcagaaaag
     6001 gattccattg caatttatcg ctagaagttc ttggcagaaa tatagtattt tacaacccgt
     6061 gtctatttcg aagtggtata atgccgatgc tttttagcaa aaatatactc tccgagggag
     6121 aagaatgaaa ctccacgaat accaatccaa aaatattttt tcgaaatttg gcatcccaat
     6181 ccccaaggga cgcgttgcga ctaccgctca agaggcgaag cagatcgccg aagagcttgg
     6241 tggtcgcgtt gtcattaaat cgcaggtgtt ggtaggcgga cgcggcaaag ctggcggtgt
     6301 aaaacttgcc aaggaccccg ccgaagccga acagctcgcc actcagattc tgggcatgga
     6361 gatcaaaggt ctccccgtgc gcaaagtctt ggtggatgaa gcctctgcca tcgaccagga
     6421 aatctacttc gcgatcaccg atgaccgcgc cgctaagaaa ccggttctta ttgcctctgc
     6481 tgccggtggt gtggacattg aagaagttgc cgccaagacc cccgaaaaaa tcattaaagt
     6541 aaacattgac cccttgcttg gcttacgcga atatcaggct cgtgacgttg ccgttgccat
     6601 tgaccttcca cgtgattatt ggaaagattt caccaaaatc gccatggggc tttggcatgt
     6661 ctatcaaagc accgacgcct ccctcgcaga aatcaaccca ttggttatca ccaaagataa
     6721 gaaaatgatc gctctcgacg gcaaaatgat gatcgacgat aatgctctgt ttcgtcaatc
     6781 agatttggct gagattcgcg ataccgatga agatgcacct gctgaaatcg aagctcgcaa
     6841 gtatggtctg tctttcatta agttagatgg caatatcggc tgtatggtca atggcgcagg
     6901 tctggcaatg accagcatgg acgtaatcaa actctttggt ggcgaccccg ccaacttcct
     6961 cgatgttgga ggcggtgctg gcgcagagaa agtcgctgct gccatgcgca tcatcctgac
     7021 tgacccgaac gtcaaagcag tgcttttcaa tatcttcggc ggcatcaccc gctgtgacga
     7081 agttgccaag ggcattctcg ttgccatgga cgaagtcaag ccgaaggtgc cgatggtcgt
     7141 tcgccttgtt ggaacgaatg cagaagaagg tcgccgtttg cttgaaaatg caaacatgat
     7201 cactgccgaa actctcgccg acgccgccaa gaaagccgtc gctgcggcaa agggataata
     7261 caaatgagca ttttagtaga taaaaatacc cgccttcttg tgcaaggcat cactggcaac
     7321 gaaggcttat tccacaccac gcagatggct gcatatggca ccaatatcgt cggtggaatc
     7381 acccccggca agggcggtga atgggtcttg gatggaaaga tccccgtctt tgattcggtc
     7441 aaatctgcca a
//
LOCUS       DAOQFT010000035         7443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4822_length_7443_cov_5.907553, whole genome shotgun sequence.
ACCESSION   DAOQFT010000035 DAOQFT010000000
VERSION     DAOQFT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4822_length_7443_cov_5.907553"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2564
                     /locus_tag="PLR93_01640"
     CDS             <1..2564
                     /locus_tag="PLR93_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNH25686.1"
                     /translation="GDSSVAVGGSVTDSAIIHGQTIVLADRFWRDLKPALPPDAIKEA
                     TAAFLAYLTDRHYYLSLKGMGVSDRVPLRLKLLDLYVPLKARMELPEGETWKRDLSLA
                     GRDIAEYEEPLHFGDPVPLLNVLKSYSGVVVLGDPGAGKTTFVKYLAVRLARGEGAKM
                     GLDDYLPILLPLAAFANALQSDDIRLDDFIADYFSGIGADLPIGPMLKEALKAGRALI
                     LLDGLDEVRDINMRNTVVERVVDFYTFHRHQGNKFVLTSRVVGYRAVRPSAEDLAECT
                     IVDFEDDEIEDFVERWTTALEKQAQGHTQVAQADAEADRRDLLDAIQHNAGVRQLAST
                     PLLLTILALMKRQGVTLPERRVQLYDQYVSTLLSTWNRARSLSGRAPGRDIDEIQTVR
                     ILAPLALWMHEVSPGVGLVGREDMRRKLEELFHERGDLSPIQAARQFLMDVREHAALL
                     LERGPGEYGFIHLTFEEYLAAVALALMGQGNSQPIIETLSRHVGEQAWREVTLLTIGY
                     LGIRQQLPKIAGEVVDALVDVQPGPPGEAVVLAGDAVLDTYPNGVPLKSRDHVIEALV
                     NTMQDGNIRPELRRHSGILLGRLGWRPGDLDRFVEVPAGEFQFGVRKEPKNIPQMYFI
                     AKYPVTNIQYARFVKEDGYQNREFWSDLGWEWRTGKFDQRTMEAIERDWLEHRPLAKR
                     STPYYWHNIDVSNPIVPVVGVCWFEAEAYCKWLSKKIVAVPEGYTIRLLTSEEWERAA
                     RGTDGREYPWGDGFIKSAANTWDSDATGSGLGGSTAVVTFAQGSSPLDVWDMSGNVWE
                     WTGSWYDEDRKYRIVRGGSWIGYQWFARSSFCNWSIPLMFNDDLGFRVAIAPKIA"
     gene            2573..2968
                     /locus_tag="PLR93_01645"
     CDS             2573..2968
                     /locus_tag="PLR93_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25687.1"
                     /translation="MAQKSKQGSHVSAGDGSIVIGGDVSGSNVSLFNNNVKLTPLFKT
                     IEKAVEKNNNLAPSDKADVTAELQEIRNELESTAPDETFLARRFRNIKRMAPEIVEVA
                     FETLKNPVGGVAEVVKRVAAKMAEDANAK"
     gene            3018..3698
                     /locus_tag="PLR93_01650"
     CDS             3018..3698
                     /locus_tag="PLR93_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25688.1"
                     /translation="MKRTFFSLLLVLTLTACFAPVDALPAPTAIASDTPQIAIPSPTI
                     LPATAIPAVDTPTPQVATSGFCQDARNLQLIKDLRTAIQNRDGELLAALVNPTTGVGV
                     RFIRNGNVITYFDNIKFIFETTYAADWGLAAGSGEPVKGSFQEIVLPSLDRAFTANAV
                     ITCNELQVGGATYTPEWPYADMDFYSVHTPGTDQYAGMDWETWAVGIVQQDGKPMLAA
                     LIHYAWEP"
     gene            3841..4260
                     /locus_tag="PLR93_01655"
     CDS             3841..4260
                     /locus_tag="PLR93_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNH25689.1"
                     /translation="MSCADEYTPQLRARGYRMTSQRLAILHVLHHTGKHLSPVEVYDQ
                     ARTELPSLTETTVYRTLEFLAENGLVRPALTSSGHLAYEIARHEHHHLVCRTCGSEME
                     VEHELLKSMYETLETESGYKLTDSHLTFFGLCPECQK"
     gene            4275..5213
                     /locus_tag="PLR93_01660"
     CDS             4275..5213
                     /locus_tag="PLR93_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNH25690.1"
                     /translation="MLYSPVPMHIPDGFLSLVVSIICWVVTALIVGAAISRTNRSLGE
                     KQIPLMGVMAAFIFAAQMINFPVAGGTSGHLLGGALAAIVLGPWAGMLVMTAVIAVQA
                     LLFQDGGLLVMGANILNMGLLTAAIGYGLYRSVAGQSKSVKLAVAGVAAWLSVMAGAL
                     LTSIELWLSGTSNLQIVIPAMLGVHALIGLGEALITVSALAFIMQTRPDLLGAGSESA
                     KGSPTWAYVGGAIALFVVLLSPFASADPDGLERVATDMGFINAGQSAPYQIIPDYTIP
                     FLGETAISTIVAGVVGVIVVAAIIILVGRGMKAKSS"
     gene            5272..6063
                     /gene="cbiQ"
                     /locus_tag="PLR93_01665"
     CDS             5272..6063
                     /gene="cbiQ"
                     /locus_tag="PLR93_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNH25691.1"
                     /translation="MHFDAFDRYHEKESFLHRLDPRVKVLVTVTFIISNALLPDAAWV
                     AFAFAWLFLLACNLLSKLGITYTLKRSIVAFPFALIAITILFSMPGKPLATFHLMMSE
                     YTITDMGLLRFLSILVRSWLSVQMAILLVATTRFPDLIHAFEHLRVPAVLTTIIAFLY
                     RYLFVLTDEVFRLLRAREARSAAVAGSRSGRGVMWRAKIAGNMAGQLFLRSYERSDRI
                     YNAMVARGYVGHLYTLNPHEMKSSDYFSTAAAVAIIFITHILGRF"
     gene            6063..6818
                     /locus_tag="PLR93_01670"
     CDS             6063..6818
                     /locus_tag="PLR93_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25692.1"
                     /translation="MPVTHITEQFSQPQSSNDVLSVNDLHFSYPDGHVALSGVSLKLY
                     EGDKVALVGPNGAGKSTLMLHLNGILGGKGEVEIAGMRLTRDNLPAIRARVGLVFQNP
                     DDQLFSPTVFEDVAFGPLHMGLPEAEVRARVDTALEAVRMTAYRDRLSHHLSVGEKKR
                     IAIATVLSMNPSLLVLDEPSAGLDPRARRTLIHLLRDLPITMLVSTHDMAMVKELFPR
                     TVVMDEGRVVADGPTSEILENETLLTAHGLEKP"
     gene            6900..7349
                     /locus_tag="PLR93_01675"
     CDS             6900..7349
                     /locus_tag="PLR93_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25693.1"
                     /translation="MIIKPDLGGRIFSGTAGAVLLILACTGLTIPFPGVLFICLGLPL
                     AVFAFGYAFGAAQTQIDEDGVSQRNFFFVPKKFTWNEIVSGKIASETYNSKDSSGWTT
                     RRTRTYALFVNKEGKKIYVNANSSGPEEWWDAMRRISKEKLGERFDG"
BASE COUNT         1770 a         1673 c         1934 g         2066 t
ORIGIN      
        1 cgggagattc aagcgttgcc gtaggcggtt ccgtcacgga tagcgccatt atccatggac
       61 agacgattgt gcttgcagac cgcttctggc gggacctcaa gcctgctctg cctcccgatg
      121 cgatcaaaga agccactgcg gcttttctcg cctatctcac agaccgacat tactatttga
      181 gcctgaaagg catgggggtc tcagaccgtg ttccactgag gctaaaactg ctggacctct
      241 atgttccact caaagcacgt atggaactcc cagaagggga gacttggaag cgtgacctaa
      301 gcctagctgg gcgtgatata gcggaatatg aagagccttt gcattttggt gaccccgtcc
      361 cattgctgaa tgttcttaag agttattctg gggtcgttgt attaggtgac cctggcgctg
      421 gtaagacgac cttcgtcaag taccttgctg tccgtttggc gcgtggggaa ggcgcaaaga
      481 tggggctaga tgactatctc ccgatcttgc tcccattggc agcatttgca aatgcactac
      541 agagtgatga catccgcttg gatgatttca ttgccgatta tttctctggc attggtgccg
      601 atcttcccat tggacccatg ctcaaagaag cactcaaagc tgggcgtgcg ctcattttgt
      661 tagacggact cgatgaagtc cgtgatatca acatgcgcaa tacagtggtt gaacgggtag
      721 tagactttta tacgttccac cgtcatcagg gcaataaatt tgtgctcacc agccgcgtag
      781 tagggtatcg cgcagtacgc ccctcggctg aagatttagc tgaatgcacc atcgttgact
      841 ttgaagatga cgagatcgaa gatttcgtag aacgatggac aactgctctt gagaaacaag
      901 cacaagggca tacccaagtg gctcaagcag acgccgaagc agaccgccgt gacctcttag
      961 atgccattca acataatgca ggtgtacgtc aacttgcttc caccccattg ttgctcacca
     1021 ttctggcgtt aatgaaacgt cagggggtga ccctgccaga acggcgcgta cagctttacg
     1081 atcaatacgt ctccactctt ctctccactt ggaatcgtgc ccgcagctta agcggacgcg
     1141 ctcccggacg tgacatcgat gagattcaaa ccgtgcgtat tctcgcgccg ttagccttgt
     1201 ggatgcatga agtcagcccg ggtgtgggat tggtagggcg tgaagatatg cgccgaaaat
     1261 tggaagaact cttccatgaa cgcggcgatc tttctcccat tcaagcggca cgtcaatttt
     1321 tgatggatgt gcgtgaacat gctgcccttc tgctcgaacg tggaccgggt gagtatggct
     1381 tcatccattt aacctttgaa gaatatctcg cagcagtggc tttggcattg atggggcaag
     1441 gcaattccca gccgattatt gaaactttat ctcgccatgt aggcgaacaa gcttggcgtg
     1501 aagtgacctt gcttactatt gggtatcttg gcatccgtca gcaattacca aagattgccg
     1561 gcgaagtcgt cgatgcattg gtagacgttc aaccgggtcc gcccggagaa gcggtggtgt
     1621 tggctggcga tgccgtattg gatacttatc ctaatggcgt accgcttaag agccgggacc
     1681 atgtcattga agccttggtc aacaccatgc aagatggcaa tatccgccca gagctgcggc
     1741 ggcattctgg gatattgctt ggtcgccttg gttggcgccc cggagacttg gaccggtttg
     1801 tggaagtgcc cgctggtgag ttccagtttg gggtccggaa agagccgaaa aatattccgc
     1861 aaatgtactt cattgcgaag tatccggtta cgaacatcca atatgcgcgt tttgtcaaag
     1921 aagatggata tcagaaccgc gaattttgga gcgacctggg ttgggaatgg cgcacaggga
     1981 agttcgatca gcgcacgatg gaagcgattg aacgtgattg gctggagcat cggccgctgg
     2041 caaaacgcag cacaccgtat tattggcata acattgatgt aagcaaccca attgtgccgg
     2101 tagtgggggt gtgttggttt gaagctgaag cgtattgcaa atggctttcg aaaaagattg
     2161 tggctgtgcc agaaggctat accatacgtt tgctcaccag cgaagaatgg gaacgtgcgg
     2221 cgcgcggtac agatggacgt gaatacccat ggggcgatgg gtttatcaag tctgctgcca
     2281 acacatggga tagcgatgca accggttcgg ggctgggtgg tagtactgct gtggttacct
     2341 ttgctcaagg tagtagtcca ttggatgttt gggatatgag cggtaacgtt tgggagtgga
     2401 caggttcttg gtacgacgaa gatagaaaat atcgcattgt gcgtggcggt tcatggattg
     2461 gttatcagtg gttcgcgcgt tcttcttttt gtaactggtc gattcctttg atgttcaatg
     2521 atgatctagg ttttcgtgtg gcaattgcac ccaagattgc ataggagtaa atatggcaca
     2581 aaaaagtaaa caaggaagtc atgtaagcgc tggcgatggc agtattgtga tcggtggaga
     2641 tgtaagcggt agcaacgttt cgcttttcaa taacaatgtg aaactcacac ctttgtttaa
     2701 gaccatcgaa aaagcggttg aaaaaaataa taatctcgcc ccttcagata aagccgatgt
     2761 gactgccgaa ttacaagaga ttcgcaacga gctggaaagc actgcccccg atgaaacttt
     2821 tcttgcccgg cgcttccgaa acatcaaacg catggcaccc gaaattgttg aagttgcctt
     2881 tgaaacattg aaaaatcctg tgggtggtgt ggcagaagtg gtcaagcggg ttgcggcaaa
     2941 gatggctgaa gacgcaaacg caaaatagta caatgaactg gcggagaccc cgccagtttt
     3001 cttttgaaag gatacccatg aaacggacgt ttttctctct gttacttgtt ctgaccctta
     3061 cggcttgctt tgcccctgtg gatgcacttc cggctccaac tgccattgct tctgatacac
     3121 cccaaatagc aattccctcc ccgactattt tgcctgccac cgcaatccct gcagtggata
     3181 ctcctacacc acaagttgct acatcaggtt tttgccaaga tgcgcgaaac ctgcaactca
     3241 ttaaagattt acgtaccgcc attcaaaacc gtgacggtga attgctcgct gccttggtca
     3301 acccaactac gggcgtaggt gtgcgtttta ttcgtaatgg aaatgtcatc acctactttg
     3361 ataacatcaa atttatcttt gaaactactt atgccgcaga ttgggggctt gctgctggta
     3421 gtggggaacc ggtgaaaggc tcttttcaag agattgtctt gccttcccta gaccgagctt
     3481 tcactgcaaa tgcggtgatt acctgtaacg aactccaagt aggtggtgca acctatactc
     3541 cagaatggcc ttatgcagat atggactttt actccgtcca cacgccgggc acagatcaat
     3601 atgccggcat ggattgggag acttgggcag taggcattgt gcaacaagat ggcaaaccca
     3661 tgcttgccgc cctcatccac tatgcgtggg agccgtagta atctaaagac agactaagaa
     3721 aacatggacg gggcaatatg tggggacatt atcccgtcca tgtgctgttt aatgtaagaa
     3781 aaccctgtct gtgatattct aagaactata ctattgcaag aagttgcaat aaggagagct
     3841 atgtcttgtg ctgatgagta caccccacaa ttacgcgccc gcgggtaccg catgacctct
     3901 caacggttgg cgattctgca cgtgcttcat cacacaggca aacacctctc gccggttgag
     3961 gtttacgatc aggctcgcac tgaacttccc agccttaccg aaaccactgt ctatcggacg
     4021 ttggaatttc tggctgagaa tggcttggta cgcccggcat tgaccagttc tgggcacctt
     4081 gcctatgaga tcgcccgaca tgagcaccat catttggttt gccgcacctg tggaagcgaa
     4141 atggaggtgg aacacgaatt acttaaatcc atgtatgaaa ccttggaaac cgaaagcggc
     4201 tataagttga ccgatagcca ccttactttt tttggtcttt gtccagagtg tcaaaaataa
     4261 aaggagagat tcacatgttg tattccccag ttccaatgca tatccccgat ggtttcttga
     4321 gcctggttgt ttcgatcatc tgttgggtcg taactgcctt aatcgtgggt gctgcgattt
     4381 cccgcacaaa tcgttcctta ggtgaaaaac agatcccgtt aatgggtgtg atggcagctt
     4441 ttatcttcgc tgcgcagatg attaacttcc cggttgcagg tgggacatct ggtcatcttt
     4501 taggcggtgc tttggcggcg atagttttag gtccatgggc aggcatgtta gtgatgacgg
     4561 ctgttattgc ggtgcaagcg ttgctatttc aagatggcgg tctgctcgta atgggtgcga
     4621 atatcctcaa tatggggctg ttaacggctg ccattggtta tggtctttac cgttctgtgg
     4681 cagggcaatc aaaatcggtc aagttggcag ttgctggggt tgccgcctgg ctttctgtca
     4741 tggcgggagc tttattaacc tccattgaac tttggcttag tggaacttcg aatttgcaga
     4801 ttgttatccc tgccatgttg ggtgtgcatg ccctaattgg gttgggtgag gcgttgatta
     4861 cggtttcagc gttggcattc attatgcaaa cccgaccaga tttgcttgga gcaggttctg
     4921 agtctgcaaa aggaagccca acttgggctt atgtgggtgg tgccattgct ttatttgtcg
     4981 tattactttc tccctttgct tcagcagacc cagatggctt ggagcgcgtt gctacagata
     5041 tgggctttat caatgcaggg caatcagcgc catatcagat tattccagat tacacaattc
     5101 cattcctagg tgaaactgcc atttctacaa ttgtagctgg tgtagtgggt gtgattgtag
     5161 ttgccgctat tattatctta gttggtcgtg gaatgaaggc gaaatcttca taagttgata
     5221 tagaatcgag gtgcgtaaat tattaattta cgcacctcgg aataggttta tatgcatttt
     5281 gatgcctttg accgttatca tgaaaaggaa agttttcttc accggctcga cccgcgtgtg
     5341 aaggttttgg taacggttac ttttattatt tcgaacgcac tattgccaga tgctgcatgg
     5401 gttgcttttg cctttgcttg gcttttctta ctggcttgta acctgctttc aaagcttgga
     5461 attacataca ccctcaagcg atctattgtc gcttttccct tcgcattgat cgctattact
     5521 atactttttt caatgccagg aaaaccgctc gctacatttc acttgatgat gtctgagtac
     5581 accatcactg atatgggtct gcttcggttt ttgagtatct tggttcgttc ttggctatcg
     5641 gtgcagatgg cgatcctttt ggtggctacc acgcgctttc cagatttgat ccatgctttt
     5701 gaacatttac gcgtcccagc cgtgttgaca acgatcattg cctttcttta tcgttatctg
     5761 tttgtgctaa cggacgaggt tttccgtctg ctgcgggcga gggaagcgcg ttcggcagca
     5821 gtagcaggct caaggtctgg cagaggcgtg atgtggcgtg cgaaaattgc cgggaatatg
     5881 gcgggtcaat tattcctacg tagttacgaa cgcagtgacc gcatctacaa tgccatggtt
     5941 gctcggggat atgtgggtca tctctatacg ttgaatccgc atgagatgaa atccagcgat
     6001 tatttttcga ccgcagctgc tgtagctatt atttttatca ctcatattct tggaaggttc
     6061 taatgcctgt tactcatatt accgaacaat tttcacaacc tcagtctagc aacgatgtgt
     6121 tgagtgtaaa cgacttacat ttttcatacc ctgatgggca tgttgccttg agtggcgtat
     6181 cccttaagtt atatgaagga gataaggtcg cattggtggg cccgaatggg gcggggaagt
     6241 ccacgttgat gctgcacttg aatggaatct taggtggtaa aggtgaagtg gaaattgcgg
     6301 gtatgcgcct cacacgtgac aatttgcctg ccatccgtgc gagagtgggt ctcgtattcc
     6361 agaacccaga tgaccaactt ttttcgccca ctgtatttga agatgtagcc tttggacctt
     6421 tgcatatggg gttgcctgaa gcggaagtcc gcgcacgagt ggacactgcc cttgaagcgg
     6481 tacggatgac agcctatcga gatcgtctgt ctcatcactt gagtgtaggc gagaagaagc
     6541 gcattgccat tgcaacagta ctttccatga atcctagtct gctggtgttg gatgaacctt
     6601 cagccgggtt agacccccgt gcgcgcagaa cgctgattca ccttttgcgc gatttgccga
     6661 tcactatgct ggtgtctacg catgatatgg cgatggtgaa agaactcttc ccacgcactg
     6721 tagtgatgga cgaggggcgg gttgttgccg atggaccaac ctcagaaatt ctggaaaatg
     6781 aaacattgct tacggctcat gggttggaaa agccataact aatcaatagg aaagatgggt
     6841 actccccatc tttttttatt tttactagaa tatgggtatt tcaatctcaa ggagttccca
     6901 tgattataaa accagacctt ggcggacgta ttttttctgg aacggctggc gccgtccttc
     6961 ttattttggc ttgtacagga ctaaccattc ctttccctgg cgtgctcttt atctgtttgg
     7021 ggttgccctt ggctgtgttc gcttttggct atgcatttgg cgctgcacaa acccagatcg
     7081 atgaagatgg tgtttcacaa agaaactttt tctttgtgcc caagaaattc acatggaatg
     7141 aaatcgtaag tggaaagatt gcatccgaaa catataactc aaaagatagc tcagggtgga
     7201 ctacccgccg tactcgaact tatgctttgt ttgtaaacaa agaaggcaaa aagatttatg
     7261 tcaatgccaa tagttcgggt cctgaagaat ggtgggatgc gatgaggcgg atatccaaag
     7321 agaagttagg ggagcgattt gacgggtaag ttgaagatat aaaaagagct tctgaatttt
     7381 cagaagctct ttttataaag tctgtattac aacccagcag cacttttcaa agcagcagct
     7441 ttg
//
LOCUS       DAOQFT010000036         7416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4848_length_7416_cov_7.017389, whole genome shotgun sequence.
ACCESSION   DAOQFT010000036 DAOQFT010000000
VERSION     DAOQFT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4848_length_7416_cov_7.017389"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLR93_01680"
     CDS             <1..622
                     /locus_tag="PLR93_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNH25694.1"
                     /translation="YPGRLLEMVNAVRPDSTLSNFGQSGWSSDALINGDQGLESQLTR
                     AVSELQDATDQGKATLALVWIGSNDLWYLYEYGGDIDDAGDASDAQRFEANIDTILSQ
                     LRGTGAQVVIALLDDQSKRPVALKGEAFVSITPDEMNRMSAHVDVYNQIIRDKAAQYG
                     ALTVDFYSTDIFTNPATLYDDGNHPNQAGYDIITQKWFDAISPLLK"
     gene            complement(663..1673)
                     /locus_tag="PLR93_01685"
     CDS             complement(663..1673)
                     /locus_tag="PLR93_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNH25695.1"
                     /translation="MKKIFPFLFLALLLSSCISVEVKEADTPVPSNFVTATLAPTKAG
                     YVPPTFTETPAITQTPTFAVTIDPNCQDTAVLVRDVTVPDGTQMKPGEKFTKTWEFIN
                     SGDCPWYGYTIKFAAGDQMNAPLSAPIPDTLIKKSVQVSVDLTAPTTNGSFTGYFTLN
                     NPTGKDVPIGIEKTFWVKITVGSGSPAPQATVSSSTQLSSGSVGANCNYSQNAGYVNQ
                     IASLINQERANAGLPALTINSLLTNAAQGHAADMACNKNISHTGSDGSSAYARILASG
                     YAPSYAEEIIYGGGGPQAAMTWWMNDQIHRDAILSAKSSEMGVGYANYSGDYIVVDFG
                     SP"
     gene            complement(1715..3913)
                     /locus_tag="PLR93_01690"
     CDS             complement(1715..3913)
                     /locus_tag="PLR93_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25696.1"
                     /translation="MESQPYIPGFKPVDVGPLSRFLPALEDGTISGWLSGQTLAGNWL
                     LDPFGFSPKLVLEAARSGYRVLVTANNPITRFLLEVFANPPPQADFVAALADLGAVKK
                     GDERLEQHLQSLYLTQCEKCEKQIYAQAFLWRKGEDVPYAKLYECPHCKDSGERITTD
                     SDKDIALQIAGTDTLHRTRLFERVVSLKDEDRVYAEEAIEHYLPRPLYVLGTILNRLD
                     SLDLNESRRRALTALLLLAFDAGNTLWGHPAERPRPKLLNTPSQFRENNLWIMLERGI
                     RLFAESGSPVVIEAWPKKIPETGGICIYEGRLKDLAHEVKREIPIAAVVASIPRPNQA
                     FWTLSALWAGWLWGSDAVEPYKVALRRRRYDWAWSATALFAMFSHLDELLADGVPFFG
                     LMPEPEAPFMTSALTAASAAGFKLESVALRTEHDPVQVLWKSGKRSASAEIEVETARN
                     AMRSYIAARGEPAGYLHLHTAGLIALAEVNALKQVNDEFDFAMRKTQSVMEQALKDSH
                     EFVHYSTGEGVDTGVWSLASGHAESLSDKIEVGIVNYLQKNPQTIFLEVEEELNKQFT
                     GLMTPSKGLIYAILNSYAEKDGGIWNLRNEDYASARRDEMKTVFSLLEEIGKRLEYKT
                     KIEEKIVIWFEAGKTVRKFYVMASALIHRALEQIDEQTVIVIPGGRAALAAYKQERDP
                     SLKERLKKHKLAKYRLLRSVLDLPILTRETFEELITSDPVEKAENQLMMF"
     gene            complement(3915..4883)
                     /locus_tag="PLR93_01695"
     CDS             complement(3915..4883)
                     /locus_tag="PLR93_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH25697.1"
                     /translation="MNFSKFNLAVVIPAYRTEKEILAVLQGIPAFIRHIIVVDDASPD
                     SSADLVTAAAKKNRRILLVRHAKNQGVGGAMVTGFKKALELGAEIVIKLDGDGQMDPQ
                     YIPALITPLITGEADYAKGNRFRDFDALRQMPIVRRIGNLGLSFLTKAATGYWNIFDP
                     TNGYFAIRAEMLAQLPLDRIDKGYYFETSMLSRLYLRDAFVQDVTIPARYRNEVSSLS
                     IRRVLFEFPYKLTRTLIKRIILKYFIFDFSMMSIYLLTGIPLLLFGLIFGITKWIQYA
                     ELGIAAPTGTVILPTLSVILAIQILLSAIEIDLNAAPRKAISKALV"
     gene            4936..>5315
                     /locus_tag="PLR93_01700"
     CDS             4936..>5315
                     /locus_tag="PLR93_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25698.1"
                     /translation="MATGERILIVESDPDIADLIGRQALKPLGYQVTVVGDAGSAIKF
                     AAQTPPDLILANINLPGLSGKDLLAALNSQNIKSPVIVIAEKGQEPDAIQAFRLGAMD
                     VMFWPLRDAEAVSIVERAMRQTQEA"
     assembly_gap    5316..5415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5416..5949
                     /locus_tag="PLR93_01705"
     CDS             <5416..5949
                     /locus_tag="PLR93_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25699.1"
                     /translation="GLPEAWAKKMNQPMDDGISSLVALSGESLFMNGSALQKFKIASL
                     GKSAGVVPIKIKNEVIGLMIVVRKGEKEFVKDSQTMLEAMADYASISLVNARLFRALE
                     QTAENARTGEKARYASLETLRKSIRTEVESAVYPLNLVLTEMPGALNPEQKKALETVK
                     TALQKLSHASENTVTSK"
     gene            5968..7416
                     /locus_tag="PLR93_01710"
     CDS             5968..7416
                     /locus_tag="PLR93_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH25700.1"
                     /translation="MSKQDLILLALDASPTLDLMVRAFHSANYEVAVVHDQSGLEKSI
                     QQAIPALMIIGEIFNGEDGFSIATNTLERFPTMPIILYAEKDKPGLAQHIIKAGISGY
                     LYPPLKLDDIVEEVKRTLIRARKLGDWLRREVKRTTASLEQKAQISEAERNKLDAIIS
                     NIQDGVLVLDETHHILLANKTIYEIFSLNGNPILGKYVKDVINSADLNALIDRARNGA
                     LKYHEINFDDGRVYNAQYTPLPRIGSAVTMQDISYLKEIDRLKNDFVHTVSHDLRSPL
                     TSVLGYMELIERVGPLNESQHEFLERLRAGVHHMTSLVNELLDLGRLESGFDTRRETV
                     NLRNVLEYSLNVFDNQIKKKNLQVTCEFADNLKSLRANPIRIRQMIDNLVGNAIKYTP
                     QNGAIKLNLAMQETQVVFRVTDSGPGIPPDEQNRVFEKFFRASNRSSEIEGSGLGLAI
                     VKSIVESHQGRVWVESKVGIGSTFIVLLPATE"
BASE COUNT         1959 a         1769 c         1737 g         1851 t
ORIGIN      
        1 atatcccggt cgcctgttag agatggtcaa tgccgttcgc ccagattcga ctttgtcaaa
       61 tttcggtcag tccggctgga gttctgatgc gctcatcaac ggtgatcagg gattggaaag
      121 tcagcttacg cgtgcggtga gtgaattgca agatgcaact gaccaaggca aagcgactct
      181 ggcattggta tggatcggca gcaacgacct ttggtatttg tacgaatacg gtggcgacat
      241 tgacgatgcg ggtgacgctt cggatgcgca acgcttcgaa gcaaatattg acaccatcct
      301 tagccaactg cgtggaacag gcgctcaagt ggtcattgct cttttagacg accaatccaa
      361 acgcccggtg gcactgaaag gcgaagcttt cgtttccatc acgccggatg aaatgaaccg
      421 catgtctgcg catgtggatg tctacaacca gatcattcgc gataaagctg cccaatatgg
      481 cgcactgact gtggacttct actcgaccga tattttcacc aaccctgcta cgctctacga
      541 cgatggcaat catcccaacc aagctgggta cgatatcatc acccaaaaat ggtttgacgc
      601 catatcgcct ctgctgaaat agagaaacaa aaagtctctt gcgtttgcaa gagacttttt
      661 gtttatgggc tgccgaaatc taccacaatg taatcgcctg aataattggc atacccaacg
      721 cccatttctg acgattttgc actaagaatt gcatcgcgat ggatttgatc gttcatccac
      781 caagtcatcg ccgcctgcgg accacctccg ccgtagataa tttcttccgc atacgatggt
      841 gcatatcctg aggcaagaat tcgagcatat gctgaagagc catctgaacc cgtatggcta
      901 atatttttat tacatgccat atccgcggcg tgaccttgtg ctgcattcgt cagcaaggaa
      961 ttaatcgtca aagcaggcaa accggcattc gcccgttctt gattgatcaa cgatgcgatt
     1021 tgattaacat aaccagcatt ttgactgtaa ttgcagtttg cgcctacact acctgaagac
     1081 aattgcgtgc tactagaaac cgtcgcctgt ggagctgggc ttccactgcc aacagtgatc
     1141 ttcacccaaa acgttttttc aataccaatt ggcacgtctt tacccgttgg gttgttgagc
     1201 gtgaagtaac ctgtaaagga tccgttcgtg gttggggcgg tcagatccac tgaaacttgg
     1261 acagatttct tgataagcgt atccggtatc ggcgcggaaa gaggcgcatt catttgatcc
     1321 cctgccgcaa atttaatggt gtatccatac cacgggcaat cgccgctgtt gatgaattcc
     1381 caagttttgg tgaatttttc accgggcttc atctgcgttc catcagggac agtcacatcg
     1441 cggacgagca cggcggtatc ctgacagttc gggtcgatgg tgactgcaaa ggtaggcgtt
     1501 tgtgtgatcg ccggagtctc ggtgaaagtt ggcggaacat accccgcctt cgtaggagcc
     1561 aaggttgccg ttacaaaatt agaaggcacg ggggtatctg cttctttcac ctcgaccgaa
     1621 atacaggaag ataaaagaag tgcgagaaaa agaaaaggaa atattttttt cattgagttt
     1681 taccaaagct attttcgttc accgatgaag tgagttagaa catcatcaat tgattttcgg
     1741 ctttttccac cgggtcactg gtgattagct cttcaaatgt ttcgcgagtc agaataggca
     1801 ggtctaacac actgcgcaat agacgatatt ttgccaattt atgtttcttc aatcgttcct
     1861 tgagtgaagg gtcgcgttct tgtttatagg ctgccaacgc tgcccgcccg ccagggataa
     1921 cgatgacggt ctgttcatca atttgttcca acgcacggtg gatcaatgcc gaagccataa
     1981 cataaaattt acgaacagtt tttccagctt caaaccaaat gactattttt tcttcaattt
     2041 tagttttgta ttccaggcgt ttcccaattt cttccaacaa tgagaaaact gttttcattt
     2101 catcacggcg agctgacgca taatcttcat tgcgcaaatt ccaaattccg ccgtctttct
     2161 ctgcatagga attcaggatg gcatagatca aacctttgga tggagtcatc aagcctgtga
     2221 actgcttatt caattcttct tcgacttcca aaaagatcgt ttgcggattt ttttgcagat
     2281 agttcacaat gccaacttcg atcttgtctg aaagcgattc agcatgtccg cttgccaagc
     2341 tccatacacc agtatcaacg ccttcgcccg tagagtaatg aacaaattca tggctatctt
     2401 tcagggcttg ttccatcaca ctttgagtct tgcgcatggc aaagtcaaat tcatcattga
     2461 cttgcttcaa tgcattgacc tccgccaacg cgatcagccc tgcagtatgc aaatgcaaat
     2521 accccgccgg ttcaccacgt gctgctatat agctacgcat tgcattacgt gccgtttcta
     2581 cttctatttc agcagatgct gaccttttgc cgcttttcca taacacttgc acggggtcat
     2641 gttcagtgcg caatgcgaca ctttctaatt tgaaccccgc cgccgatgcc gcggttaaag
     2701 ccgaagtcat aaaaggtgct tcgggctcag gcatcaaacc aaagaacggc acaccatctg
     2761 caagcaattc atccaagtga ctgaacatcg cgaacaaagc tgtggcactc catgcccagt
     2821 catatcgcct gcgtcgcaac gctactttat atggctccac cgcgtcactg ccccacaacc
     2881 agcctgccca caatgccgaa agagtccaaa acgcctgatt gggtctcgga atagaagcca
     2941 ccaccgccgc aataggaatc tctcgtttga cttcatgggc aaggtctttc aagcgacctt
     3001 catagatgca gataccgccc gtctctggaa ttttcttggg ccacgcttca atcacaacgg
     3061 gtgagcctga ttcagcaaac agacggatgc cacgctcgag catgatccac aaattgttct
     3121 cgcggaattg acttggtgtg ttcagcaact tcgggcgcgg acgttccgcc ggatgccccc
     3181 aaagcgtatt gccagcatcg aaagccagta acaacaaggc ggtcaacgca cggcgacgtg
     3241 actcgttcaa atccaaacta tccaaccgat tcaaaatggt acccaacaca tacaacggac
     3301 gcggcaaata atgctcaatg gcttcttccg catagacacg gtcttcatct ttgagcgaaa
     3361 ccacacgctc gaagagacgt gtacgatgca gagtatctgt ccctgcaatt tgcagcgcga
     3421 tgtctttatc tgagtctgta gttattcgtt cgccagagtc tttgcaatgc gggcactcat
     3481 ataattttgc atagggcacg tcctcgcctt tgcgccagag gaaagcctgt gcataaatct
     3541 gtttctcgca tttttcacat tgcgtaaggt aaagggattg tagatgttgt tcaagtcgtt
     3601 cgtccccttt tttcacggcg ccaagatcgg caagcgcagc gacaaagtcc gcttgcggtg
     3661 gcgggttggc aaagacttcc agcaaaaagc gtgtgatggg attattggct gtgacgagca
     3721 cacggtatcc gctacgggcg gcttcgagta caagcttcgg ggagaatccg aatgggtcga
     3781 gcaaccagtt cccggctaga gtctgacccg agagccagcc tgaaatggtg ccatcttcca
     3841 gcgcaggtaa aaagcgcgag agtggaccaa cgtctacggg tttgaatccg ggaatgtaag
     3901 gttgagattc catatcacac cagcgctttg ctgatggctt tacgcggagc ggcgttgagg
     3961 tcaatttcga tggcagaaag caaaatttga atggcgagaa tcaccgagag cgttggcaaa
     4021 atgaccgtgc ctgtgggcgc agcgattcct aactcggcat attgaatcca tttggtgatg
     4081 ccaaagatca acccgaaaag caacaacgga atgccggtta gtaaatagat cgacatcatc
     4141 gaaaaatcaa agatgaagta tttgaggatg atgcgtttga tgagagtgcg ggtgagctta
     4201 tatggaaatt caaacagcac acgcctgatc gaaaggcttg aaacttcatt tcggtagcgt
     4261 gcaggaatgg tcacatcttg cacaaaggca tcgcgcaggt ataagcgcga gagcatagat
     4321 gtttcaaagt aatagccttt atcaatgcgg tcgagcggga gttgcgccag catttcagca
     4381 cgaatggcga aatacccatt ggtggggtca aaaatattcc aataccccgt ggcagctttg
     4441 gtgagaaagc tcaagccaag gttgccgatg cgccgtacga ttggcatttg ccttaatgca
     4501 tcaaaatcgc gaaagcgatt gcctttggca tagtctgctt caccggtaat gagtggcgta
     4561 atcagcgcag ggatgtattg tgggtccatt tgcccatcgc catcgagttt tattacaatt
     4621 tcggcaccaa gttctaaggc tttcttaaaa cctgttacca tcgcgccacc tacaccttga
     4681 ttttttgcgt gacggacgag gaggatgcgt cgattctttt tggcggcagc agtgactagg
     4741 tccgcgctag aatcgggcga agcatcatcc actacaataa tgtggcgaat gaaagcagga
     4801 attccctgta gaaccgcgag aatttctttt tcggttcgat aggcaggtat cacaacagca
     4861 aggttgaatt tcgaaaaatt catcttcaag attgtaatcg aaaaggtgat agaatctttt
     4921 gaaggagatt tacacgtggc taccggggaa cgcatcctca ttgttgaatc agacccagat
     4981 attgccgatt tgatcgggcg gcaggcgctt aaaccactcg gatatcaagt gaccgtggtg
     5041 ggcgatgccg gctctgcgat caaattcgcg gctcagactc cgccagacct gatcctcgcc
     5101 aatatcaatt tacccggctt gagcggaaaa gacctactcg ccgcgttgaa ctctcagaac
     5161 ataaaatccc ccgtcattgt tatcgctgaa aaaggacaag aaccagacgc cattcaagct
     5221 tttcgccttg gcgccatgga cgtgatgttc tggcccctgc gcgatgcaga agccgtctcc
     5281 attgtggagc gtgccatgcg tcagacgcag gaggcnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnngggct ccctgaagct tgggcaaaga agatgaatca accgatggat
     5461 gatggcatta gttcattggt ggcgctttcc ggcgaaagtc tgttcatgaa cggctccgcg
     5521 cttcagaaat ttaagattgc atcgttaggc aagtctgctg gcgttgtccc tatcaagatc
     5581 aagaatgaag ttattgggtt gatgatcgtt gtgcgcaagg gtgaaaagga atttgtgaaa
     5641 gactcacaaa ccatgcttga agccatggca gactacgcat cgatctcatt ggtgaatgcc
     5701 cgcttgttcc gtgctcttga acaaaccgcc gaaaatgctc gcactggtga aaaagcccgt
     5761 tatgcttctt tggagacact gcgcaaatcc attcgtacag aagtagaatc tgcggtctat
     5821 ccgctcaacc tggtgttgac agaaatgccc ggcgcgctaa acccagaaca aaagaaagca
     5881 ttagaaaccg tgaagaccgc tctgcaaaaa ctttctcatg cttctgagaa tacagtcaca
     5941 tccaagtaag gatatacaaa gaaagtaatg tcaaaacaag acctgatttt gctggcattg
     6001 gatgcatcgc ccacgttgga tttgatggtg cgtgctttcc actccgcaaa ttatgaagta
     6061 gcggtagtcc acgaccagtc aggattagag aaatcaatcc aacaagccat tccagcattg
     6121 atgatcattg gggaaatttt caatggagaa gatggctttt caattgccac aaacacacta
     6181 gaacgttttc ccaccatgcc tatcattctc tatgcagaga aagataagcc tgggctggct
     6241 caacatatca tcaaagcagg cattagtgga tatttatatc cgcctctaaa actggatgat
     6301 attgttgaag aagtaaagcg cactcttatc cgcgcccgaa agctgggaga ttggcttcgc
     6361 cgagaggtaa agcgcacaac ggcttcgctg gaacaaaagg ctcaaatctc agaagcagaa
     6421 cgaaacaagt tagatgcgat catatccaat attcaagatg gtgtattagt cttggatgaa
     6481 acgcatcata ttctgttggc aaacaaaact atctacgaaa tctttagtct caacgggaat
     6541 cccatccttg gaaagtatgt caaagatgtg attaacagcg cagacttaaa cgctctgatt
     6601 gaccgtgccc gaaatggtgc gttgaaatat catgaaatca actttgacga tggacgcgta
     6661 tacaacgcac aatacactcc tctgccacgc atcggcagtg cagtgaccat gcaagatatt
     6721 tcatacttaa aagaaattga ccgtttgaag aacgattttg ttcacacagt ttctcatgat
     6781 ttgcgctccc cactgacctc tgttttggga tacatggaac taattgaacg agtgggaccg
     6841 ttgaacgagt cacagcatga gtttctggaa cgcttgcgag cgggtgtaca tcacatgacc
     6901 agcttggtta atgagttact cgaccttgga cggctcgaat caggttttga tacccgccgc
     6961 gaaacggtaa accttagaaa cgtgcttgag tactctttga acgtgttcga taaccagatc
     7021 aaaaagaaga atctccaagt tacctgtgag tttgcagata atctcaagtc actccgagcg
     7081 aatcccattc gcattcgcca gatgatcgac aacttggtag gtaatgctat caagtacaca
     7141 ccacaaaatg gtgccattaa attaaacctt gctatgcaag agacacaggt cgtctttcgc
     7201 gtcacagaca gcggtccggg catcccaccc gatgaacaaa atcgtgtatt cgagaagttc
     7261 tttcgcgcat cgaaccgatc cagcgaaatt gaaggctccg gtttggggct ggcaatcgtc
     7321 aaatccatcg tagaaagcca tcaagggcgg gtatgggtgg aatcaaaagt aggtattggt
     7381 tctacattta ttgttcttct ccctgcaact gagtaa
//
LOCUS       DAOQFT010000037         7404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4865_length_7404_cov_6.145598, whole genome shotgun sequence.
ACCESSION   DAOQFT010000037 DAOQFT010000000
VERSION     DAOQFT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4865_length_7404_cov_6.145598"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PLR93_01715"
     CDS             <1..718
                     /locus_tag="PLR93_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNH25701.1"
                     /translation="KTSPSPQRLGMQEPLMRLSVLRVINIMRISVYVDGFNFYHGLTE
                     NTKYRWCDLLKLTKQFLPNGEYGKIKLFAAHSKEYYDNLGKPERQNRYLQALKTIPGF
                     EFIPSKFSQFKKTLPLRDSPKDKPEFVHVRGYKEKGADVNLASHLISDGIKNLYDIAV
                     VFTNDSDLVEPIRVVRYDLNKKVYILLTCKNRNAWVSKELITVAKYHKLVEEDHLRVS
                     QLPDVVTRPGKPALKKPLSW"
     gene            910..1194
                     /gene="rpsF"
                     /locus_tag="PLR93_01720"
     CDS             910..1194
                     /gene="rpsF"
                     /locus_tag="PLR93_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNH25702.1"
                     /translation="MRNYELMCIIQPDLDETAMNGVLDKVKGWITEAGGSIDKAEVWG
                     RRRMAYSINKHREGQYVLLNVSMSPAATSDLERNLRYQESIMRHMLSVVG"
     gene            1231..1638
                     /locus_tag="PLR93_01725"
     CDS             1231..1638
                     /locus_tag="PLR93_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNH25703.1"
                     /translation="MSRGLNKVQIIGHLGKDPEMRYTPSGKPVTTFTVAVSRTWNSAD
                     GERHNETEWFNVVAWGTLAEICKQYLVKGQQVYVEGRLQTRRWDDKEGIKHTSVEIVA
                     NEMMMLGDRREANNQPDASPAESNGAADEEFPF"
     gene            1689..1982
                     /gene="rpsR"
                     /locus_tag="PLR93_01730"
     CDS             1689..1982
                     /gene="rpsR"
                     /locus_tag="PLR93_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNH25704.1"
                     /translation="MSDERPERPERSGGEGGGERGDRKFFAKPKFCQFCADKTLTIDY
                     KKVDLLRKYVTEEGSLRPRRQTGACAKHQRLVAAAVKQARHVALLPFANRGVD"
     gene            2088..3521
                     /locus_tag="PLR93_01735"
     CDS             2088..3521
                     /locus_tag="PLR93_01735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH25705.1"
                     /translation="MTNESGKKPAMHKKHVARLERELQQTRLILYTFFGILGVVAVLL
                     IYGWLDVNYLQLNRAVATVNDNKIVLKDFEARTRLRRQQLIDNYNQYTQYQQYFGMDM
                     SSQLNSIETQLNTPELVGTSVLDAMIDEEIIRLEAEKRGITVSEEEIDKKMEEGFNFY
                     PNGTPTPSLTPTPADLPTDPAELLQFITATPDVTATPLFTATAEFTATVESQPTAEGD
                     ATATLAPTNTAEPTVTPTATLGPTSTPEPTATPFTREAYEVLLGKTKDNITKYGFDET
                     YIREFFRNQILREKLLEVIAADVKPFEEQVRARHILVADEQTAKDLIARLQAGEDFAD
                     LARQYSSDSSSEQGGDLGWFGKGQMVAEFETAAFALQNPGDFTTEPVASQFGYHIIQL
                     MAKQERPLSASEVQNAKETALQDWLTKARDTDYTVETFDFWKQRVPSTPNFITAATES
                     AAAQKTAQAESVATLDAKNSAAATPTP"
     gene            complement(3584..4444)
                     /locus_tag="PLR93_01740"
     CDS             complement(3584..4444)
                     /locus_tag="PLR93_01740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH25706.1"
                     /translation="MHIGITAITIASLVSNFYLGWESYFSLYLIASTIIIINSVTLSL
                     RRKLIEVTALMTLFVASFIHMKTFTPLYDLNPVLVERIGLFNSGAVLFSVLFMQFHNF
                     IENENLKKKLQEMSEIDPLTGAYNRRFFNEYLDIELKRLESPSRYAPAKKTNFGIAII
                     DIDDFKMINDMYGHLVGDMVIAEVAGIIKSAIFKRDIFCRYGGEEFLVLFTNTSKSGV
                     KIAIEKIRTAIQNHVFMTHGERLNIEVTVSVGFAGFSEEANVYKLLDLADKRLYKVKG
                     AGKNGAILGE"
     gene            complement(4739..5359)
                     /locus_tag="PLR93_01745"
     CDS             complement(4739..5359)
                     /locus_tag="PLR93_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNH25707.1"
                     /translation="MINPEEAVIINSLVRQALTSMQEVMGDNGLNAVLKSCGLERFVG
                     NFPPNNLEPAIKTSQYAQLNQAIEDFYGRGGKGMLQRIGRASFQYGVREQAALLGVAG
                     VALKLMPEKQRIKFILNGMADALKKSNAQVKAWVDESGERLAYVDATCGICNSRHSDH
                     PICHLYIGSVSEAVKWATGKDYEVIETHCTAKGDEYCRFEVGNVKE"
     gene            complement(5469..6092)
                     /locus_tag="PLR93_01750"
     CDS             complement(5469..6092)
                     /locus_tag="PLR93_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25708.1"
                     /translation="MTENFIFPFEIQRSRPFHFVEETSLASARIGGTMPDGISVPLRD
                     DARYFGTFPLYDGNSRLFFSIFINGSFASFIHALNAGFQTDDRVVVIVHEKKPRSTDT
                     KYASGLSPHSIQVHDIASDLIQNDIGETVIRERHKFGGRPYSIQEPILPGSEALFAQG
                     YRQVLQIDFPNPTDGAISGSWPFGDGIFNLFWKAPFEKEPYYWYLQG"
     gene            complement(6106..6909)
                     /locus_tag="PLR93_01755"
     CDS             complement(6106..6909)
                     /locus_tag="PLR93_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25709.1"
                     /translation="MTILYKQALDLLDARSLVTSLDHDLTRLIRRELSASLGGSDDIA
                     DRVLKNILLRWNCALSAADDEVTARQKNLIVVTLPLLLHKYGIQDPSLNSKAYQAIEA
                     LNREVILSDSFYRKSSAIKELLSSPVALRSRKPARPADLTFWRTGDLVAYQIAGKFYA
                     LYIHGIVGANEAPIVEFMDMETSYRPVRDDVLGKKARGIPFKDGVLHVQHYLVYGMRD
                     MPDPANQFHLLGNMPQYKPDQTHLAPPIGMGVVTDVFRLADEVREKLFP"
     gene            complement(6977..7099)
                     /locus_tag="PLR93_01760"
     CDS             complement(6977..7099)
                     /locus_tag="PLR93_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25710.1"
                     /translation="MRGEVTFIETDNYHNKAFDPYQSIGFEVIQDVLVYRKDYS"
     gene            complement(7109..7297)
                     /locus_tag="PLR93_01765"
     CDS             complement(7109..7297)
                     /locus_tag="PLR93_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HNH25711.1"
                     /translation="MTETTQIKDGDHCEVVKGTHAGKSGTVRDIKTSKTGQVTITVVQ
                     SDGERFKTLARNVVVKKR"
BASE COUNT         2092 a         1924 c         1584 g         1804 t
ORIGIN      
        1 aaagacgtcc cccagtcctc agcggctggg catgcaggag ccgcttatgc ggctctctgt
       61 tttgagggta attaatatta tgcgaatttc agtatatgta gatggtttta atttctatca
      121 cggattgact gaaaacacaa aatatcgttg gtgtgaccta ttaaaactaa ctaaacaatt
      181 tcttcctaat ggggaatatg gaaaaattaa gttatttgct gctcactcta aagaatatta
      241 tgataattta gggaaacctg aaagacaaaa tcgatattta caggcattga aaactatacc
      301 gggctttgag tttattccca gtaaattctc gcaattcaaa aagacattac ccttaagaga
      361 ctctccaaaa gacaagccag aatttgttca tgttcgtgga tataaggaaa aaggggcgga
      421 tgttaactta gcatctcact taatttccga tggaattaag aatctatatg atattgcagt
      481 tgtatttact aacgactcag atttagttga gccaattagg gttgttcgct atgatttgaa
      541 taaaaaagtt tacatcttat tgacttgcaa aaatcgcaat gcatgggtaa gtaaagagtt
      601 gataactgta gcgaaatacc acaaacttgt agaagaagat catctaagag ttagccaatt
      661 acctgatgta gtaacaagac ccggaaagcc tgcactaaaa aagccactct cttggtagtg
      721 tagaaattct ttattaaact tttaccgttg gcttaaagtt ttcggtctgc ccccaatccc
      781 gtaaaagtga ttcctgtggt aaactttcgc cgcgaaatta ggtacgttac atttctatac
      841 gtgcctttcc gttcccggca aacccacggt ttgaacagga acaaacccat ggaaaggagg
      901 ttttcccaca tgcgtaatta tgaactgatg tgcatcattc agccagacct ggatgagacc
      961 gccatgaatg gcgtgctcga caaggtgaaa ggttggatca ctgaagctgg cggtagcatc
     1021 gacaaagctg aggtttgggg tcgccgtcgt atggcgtatt ccatcaacaa gcaccgcgaa
     1081 ggtcaatacg ttctcttgaa cgtgtccatg agccccgccg ccacatcgga ccttgaacgc
     1141 aacctgcgtt atcaagagtc cattatgcga cacatgcttt ctgtagttgg ataatccgtt
     1201 tttgcccggc attctactta ggagtgactt atgagccgag gacttaataa agtacaaatc
     1261 attggacatt tagggaaaga ccccgaaatg cgctacaccc ccagtggcaa acccgtcacc
     1321 accttcacgg tggcggtcag ccgcacctgg aacagcgcag acggcgagcg ccacaacgaa
     1381 accgaatggt tcaatgtcgt ggcgtgggga acgcttgctg agatctgcaa gcaatatctc
     1441 gtaaaaggtc agcaggtcta cgtagaagga cgcctgcaaa cccgccgctg ggatgacaaa
     1501 gaaggcatta aacataccag cgtagagatc gttgccaatg aaatgatgat gctcggcgac
     1561 cggcgcgaag cgaacaacca gccagatgcc tcccctgctg agtcaaatgg cgcagcagat
     1621 gaggaatttc cgttttagtt cttgccattc tgaacaaaag ctcagagtga catcaattgg
     1681 agtaacccat gagtgacgaa cgacccgaac gacctgaacg ctcaggcggc gaaggcggcg
     1741 gcgaacgcgg tgatcgcaaa ttctttgcga agcccaagtt ctgccagttc tgcgccgaca
     1801 aaaccctgac catcgactac aaaaaagtcg accttcttcg caagtatgta accgaagaag
     1861 gcagcctgcg cccgcgccgc caaaccggcg cctgcgccaa gcaccaacgt ctcgttgcgg
     1921 cggcggtgaa gcaagcacgc cacgtagcgt tgctgccctt cgccaatcgc ggcgtcgact
     1981 aaacatcgaa ctaacgcatc ggggcatggg caccagtcca tgccccgaat ttatatccct
     2041 gcaaaacaca aacaggggca atccctttat tacaaagcga gtatcccatg accaacgaat
     2101 cgggcaaaaa gcccgctatg cataaaaagc atgttgcccg cctcgaacgc gagctgcagc
     2161 aaacccgcct catcctttac accttcttcg gcatcctggg cgtggtcgct gtcttgctca
     2221 tctacggctg gctcgatgtg aactacctgc agttgaatcg tgcggttgcc accgtaaacg
     2281 acaacaagat cgtactcaaa gattttgaag cccgcacccg cctccgcaga caacaactga
     2341 ttgacaacta caaccaatac acccaatacc agcaatactt cggtatggac atgagctcac
     2401 agctcaacag cattgaaacc cagttgaaca cccccgaatt ggttggcacc tctgtcttag
     2461 acgccatgat cgatgaagag atcatccgcc tcgaagcaga aaaacgcggc atcaccgtca
     2521 gtgaagaaga gatcgataag aaaatggaag aaggcttcaa cttctacccc aacggcaccc
     2581 ccaccccttc tctcaccccc actccagccg acctgcccac cgaccccgca gaacttttgc
     2641 aattcatcac cgccaccccc gatgtgaccg ctacgcctct cttcacggcg acagctgagt
     2701 ttacagccac ggttgaatcc cagccgacgg cggagggcga tgcgaccgct accttggcgc
     2761 ccaccaacac tgccgaaccc accgtcaccc ccaccgccac gcttggtccc acatccaccc
     2821 cagaacctac ggctactccc ttcacccgcg aagcttatga agtgttgctt ggcaaaacca
     2881 aagacaacat taccaagtat ggtttcgatg aaacctacat tcgcgaattc ttccgcaacc
     2941 aaattttgcg cgaaaaattg ttggaagtca tcgccgcaga tgtgaagccc ttcgaagaac
     3001 aagtccgcgc gcgccacatt ctcgttgccg atgaacaaac cgccaaagac ctgatcgccc
     3061 gcctgcaagc cggtgaagac ttcgccgacc tcgcccgcca atactccagc gatagctcct
     3121 ctgaacaagg cggcgacctc ggctggtttg gcaaaggaca gatggttgct gaatttgaaa
     3181 ccgccgcttt cgctttgcag aaccccggcg atttcaccac agaacctgtc gcttcgcagt
     3241 tcggctatca catcatccaa ttgatggcga agcaagaacg cccgctcagc gccagcgaag
     3301 tgcagaacgc caaagaaacc gctctgcaag attggttgac caaggctcgc gacacagact
     3361 acaccgttga aaccttcgac ttctggaaac agcgcgttcc cagcaccccg aacttcatca
     3421 ccgctgccac cgagtctgct gcggctcaaa agaccgctca agctgagtct gttgccacac
     3481 tcgatgcaaa gaacagcgcg gctgcaacac ccaccccgta aagaattaca tcaaataaaa
     3541 aatgctccag attttctgga gcatttttta tttacagagc aagtcattcg cccaatattg
     3601 cgccattctt ccctgcccct ttcactttat acaaacgctt atctgcaagg tccagcagtt
     3661 tatagacatt ggcttcttca ctgaaacccg caaaccctac actgaccgtg acttcaatat
     3721 tcaatctctc gccatgggtc ataaacacgt ggttctgaat tgcagttcgt atcttttcga
     3781 tcgcgatctt cacgcccgat tttgacgtgt ttgtaaacaa gactaaaaac tcttcgcccc
     3841 catagcggca aaaaatatcg cgcttaaaaa tcgcggactt gatgatacct gccacttctg
     3901 cgatcaccat atcgccaacg agatgcccat acatgtcatt gatcatcttg aaatcatcaa
     3961 tgtctatgat cgcgatccca aaatttgtct tcttggcagg ggcgtatcgg gaggggcttt
     4021 ccaaacgctt gagttcgata tccaaatact cgttgaagaa acgccggtta tacgccccgg
     4081 tcagcggatc gatctcagac atttcctgaa gttttttctt gaggttctcg ttctcaataa
     4141 aattatggaa ctgcatgaac aagaccgaaa aaagaaccgc gcctgaattg aacagcccga
     4201 tccgctccac gagaaccggg ttcaggtcat agagaggcgt aaacgtcttc atatgaataa
     4261 aactggcaac gaacagcgtc ataagagcgg tcacttcaat caatttccgc ctcaaagaga
     4321 gcgttaccga attgataata atgatggtgg aagcaattag atacaggctg aaataacttt
     4381 cccatcccaa ataaaaattg gaaactaaag aagcgattgt gattgccgta atgccaatat
     4441 gcatggcaag caaatacttt ctcttttgga gaaggtaaaa attaccccca aacacaaaaa
     4501 tattgattaa ggcaaccaaa gccagatcgg tgtttttcaa ataaagttga agaacaacaa
     4561 aaaaagcatt gattaccaag cttagagaaa agaatattct taaaagagaa tattttcttt
     4621 tctcatctaa atcattcaca ttcatatcat catactcaaa ctttgttcga ttatatcccc
     4681 ccacataaaa aaccgcccct aaaaccaaga ggcggagaaa actcttacaa ttttcaaatt
     4741 attccttcac atttcctacc tcgaagcgac aatattcatc ccctttggca gtgcagtgtg
     4801 tttcgatgac ttcataatcc ttgccggttg cccatttcac cgcctcagaa acggacccga
     4861 tatacaaatg gcaaatggga tggtcactat ggcgcgagtt acagattccg caagtagcat
     4921 cgacgtatgc cagccgttca ccgctttcat ctacccaagc tttgacctgt gcattgctct
     4981 tcttgagcgc atctgccatg ccatttagaa tgaacttaat gcgctgtttt tcaggcatta
     5041 acttgagtgc cacaccggca acgcccaaca aagctgcctg ttctcgtacc ccatattgaa
     5101 aggatgccct gccaatgcgt tgcaacatgc ctttgccgcc gcgcccgtaa aaatcttcaa
     5161 tggcttgatt caactgagca tattgtgagg tcttgatcgc aggctcaagg ttgttcggag
     5221 ggaaattccc tacaaaacgt tccaatccgc aagatttcaa gacggcgttc aaaccgttat
     5281 cccccatcac ctcctgcatg gaggtcaacg cctgccgcac gagcgagttg ataatgacgg
     5341 cttcttctgg gtttatcata tgggctccta tcgttatgga tcaaagattc tttctttagc
     5401 atactcacta ttgaaagtat gtgtaatgac cttatagtac caatcttgaa cttttcaaaa
     5461 agacgttctt atccctgcaa ataccaataa tagggctcct tttcgaatgg agctttccaa
     5521 aacaaattga aaatcccatc accaaagggc caggagccgg agatcgctcc gtcagtaggg
     5581 tttggaaaat cgatctgtaa aacttgccga tagccttgcg caaacaacgc ctcacttccc
     5641 ggcaggatag gttcttgaat actgtatgga cgtccgccaa atttgtgccg ctcacgaatc
     5701 actgtttcac caatatcatt ctgaatgagg tctgaagcaa tatcatggac ttgaatggaa
     5761 tgtggactaa gcccagatgc atattttgta tcagtactgc gtggtttctt ctcatggacg
     5821 ataacaacaa ctctatcgtc agtttgaaaa ccggcattca aggcgtggat aaagcttgcg
     5881 aaagagccat taataaaaat ggaaaagaag aggcgggaat ttccatcata aagcggaaag
     5941 gttccaaaat agcgggcatc gtcacggaga gggacggata ttccgtccgg catggtaccg
     6001 ccaattcttg cagatgcgag agaagtctct tcgacaaaat ggaacggacg ggatcgttga
     6061 atttcaaatg ggaaaataaa attttcggtc atcatatgtt ccttttcaag gaaataattt
     6121 ctcgcggact tcatccgcaa gcctgaaaac atctgtcact acgcccatgc cgatgggtgg
     6181 agcgagatga gtttggtctg gtttgtattg gggcatattc ccgagcagat ggaattggtt
     6241 cgccgggtcg ggcatgtcac gcatgccata gacaagatag tgctgcacat gaagaactcc
     6301 atctttaaat gggataccgc gcgccttttt ccccaagacg tcgtccctca cagggcgata
     6361 agacgtctcc atatccatga actctacgat aggcgcttca ttcgcgccta caatgccatg
     6421 gatatacaag gcataaaatt tccccgctat ttgatacgcc accaaatctc ctgtcctcca
     6481 aaaagtgaga tctgccgggc gggcgggttt tcgtgagcga agagcaaccg gcgaggatag
     6541 gagttctttg atcgccgaag actttctata gaatgaatca ctgagaatta cttcccggtt
     6601 aagcgcctca atagcctgat aagcttttga attaagagaa gggtcttgaa ttccatattt
     6661 gtggagcagg aggggcaacg tgaccacgat caaattcttt tgcctcgcgg tcacttcatc
     6721 atcagcggca gataaagcac aattccaccg aagcagaata ttcttaagaa cccgatccgc
     6781 tatatcatca gaaccgccaa gcgaagcgga taactccctg cggatcaagc gcgtcaggtc
     6841 atggtcgagc gacgtcacca gtgagcgcgc gtcgagcaaa tccaaggctt gtttgtatag
     6901 tattgtcata cccccaagtt ttcttcaatt tcatgcaatg gtcaatcaga ctaatttcct
     6961 aactccatct tattgattag gaataatctt ttcgatacac cagcacatcc tgaatcactt
     7021 caaatccaat ggactgatag gggtcgaacg ccttattgtg ataattgtca gtttcgataa
     7081 aagtaacttc acccctcatg actttgttct atcttttctt gacaacgaca ttcctcgcaa
     7141 gggtcttaaa tcgttcccca tcggactgca ctaccgtaat agtgacttga cccgtcttac
     7201 tcgtcttaat atcgcggacg gttcccgatt tgcccgcatg cgttcctttg accacttcgc
     7261 aatgatctcc gtctttaatt tgtgtagtct ctgtcatgat actcaatcct tctgcgaata
     7321 tcattataaa tactttctca aaaatactat gtcaccctga gggagcgttc ccttcgacaa
     7381 gctcaggaca ggctttgcga ccga
//
LOCUS       DAOQFT010000038         7395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4877_length_7395_cov_7.317711, whole genome shotgun sequence.
ACCESSION   DAOQFT010000038 DAOQFT010000000
VERSION     DAOQFT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4877_length_7395_cov_7.317711"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLR93_01770"
     CDS             <1..545
                     /locus_tag="PLR93_01770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH25712.1"
                     /translation="DSIQKAGTKPNVLNLFGYTGLASLAAAAAGASVTHVDASKKSVS
                     WARENQELSGLGDRPVRWIVEDALKYVQREAKRGVKYDGVILDPPKFGRGPKGEVWEV
                     YKSLPNLLDEIRQCLSDHPLFMIVTVYAVRASAIHVAQAVEEMMKKHKGMLDSGELVT
                     REKSANRLLSQAVYARWSSK"
     gene            complement(598..1173)
                     /locus_tag="PLR93_01775"
     CDS             complement(598..1173)
                     /locus_tag="PLR93_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25713.1"
                     /translation="MSFEESNFDDNLPEDGQLPEESNNNRTFLIAAGILGGIILLSIA
                     CIVGVFLYTQNSNTQVEAQAVAASQTAEVASNFINDALTATFEASILPTATLPPTATA
                     VVNVATATSTADPNAAAAVNAGTPAAATATVGAALTQAAVAQLTIVPTTTALPQTGFV
                     EDVGVPGLAVMALAFIVVILMARRLRAVPMR"
     gene            complement(1225..2280)
                     /locus_tag="PLR93_01780"
     CDS             complement(1225..2280)
                     /locus_tag="PLR93_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25714.1"
                     /translation="MLAPVHHVIGLTTIVRERLLPISGNVIARVQQKVTASEVVAETR
                     WAREHALLDVARILGVSPTVADKLIKCKVDDKVAASAEIAVGRGLFPRSVRAPKDGRV
                     VAVGGGQVLMEIGEAKMEVRAGIPGTVVQVVPGRGVVIQSFGGLVQGIWGNGRIDSGV
                     LVNLMEGLDTVLLPNRMDVSLRGSIILGGMIKNVETLQALAELPARGLIASSIQPSLL
                     PKAREMRYPILVTDGIGSLPMNSAAYKLLSTNAKREVTLNTEPYDRYTGARPEVFIAL
                     PISAEPPMPNEVEIFAPGMQVRMRRPPSMGMIGSIVAIKPGLTVLPSGLRAQAAEVKL
                     ENDETVIVPLVNMEVVG"
     gene            complement(2284..4047)
                     /locus_tag="PLR93_01785"
     CDS             complement(2284..4047)
                     /locus_tag="PLR93_01785"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNH25715.1"
                     /translation="MPTSLVTSNSLLAIDVGAANTRAVLFDVIEGEYRFVASGVAPST
                     AEAPYKDVSEGARNAIQALQNVLKRTLLETTARSLILPSQPDGSGVDSLVVTVSAGPA
                     VRTLVVGLLKDVSLESARRLTESTYARILDTLSLSDQRKPEQQIDSILRIRPDMVVIA
                     GGTDGGASRSIQKLLEPIGLASYLMPDEKRPAVLFAGNEKLVDEVKSLVGSLATTLHF
                     SSNVRPSLEVEDIAPAELELAQMFVTIRKKQLKGLDVLDLWAGGHTLPTAYATGRMVR
                     FLSNVYGSNKGLLSVDLGASSSVIAAGFANKSVLKVYPQFGLGENLTGLLNYTTVEDI
                     LRWSTLDIPSSVILDYIHQKALYPNTIAATKEDFSIAQSVAKQALYLAMQAARKDFPK
                     EAASISPTLTPLFDPIIAGGAALNDASRPGQSLLLLLDSVQPVGISTVILDQNNLMPM
                     LGAAASQNNILPVQVLESGAFLSVGTVISPVVKTKYGAPILKAKVAYANGTEANVNLK
                     YGSLEVLPLASGEVANLTIQVSRGVDIGFGPGRGTKSLPVTGGALGVVFDGRGRPLDL
                     PSDSVRRRELIKKWNWTLGGE"
     gene            complement(4053..4760)
                     /locus_tag="PLR93_01790"
     CDS             complement(4053..4760)
                     /locus_tag="PLR93_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25716.1"
                     /translation="MPVLSLDLIAGALSFVFTLMVLSYLIGDNPLFKVAIYLFVGVAS
                     GYVAAVVFWQVLYTKLFLPIGQVLLSGAYTQGVLLIVPFLGVVFILLKAVPGMAGVSR
                     IAMAFLVGAGAAVTIAGALTGTLFPQVNATTNFFDLQAAAARNINMVEVAANGTVLLM
                     GVVTSLAYFHFGANPKADGSTKRFAPINMFAWIGRFFISVTLGAVFAGVYAAALTAFI
                     ERVSSLVFFIKTLLGML"
     gene            complement(4762..>7395)
                     /locus_tag="PLR93_01795"
     CDS             complement(4762..>7395)
                     /locus_tag="PLR93_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25717.1"
                     /translation="TQQQTQQPPKPEVPAKPAASSTDFLAGLQSQAGDDDDEEIPDWL
                     ASITGTPAPKQKPNETVEESSGTRWVETGNKSDFQQPEAEEEVPSWLTGMQQQPIPDE
                     KDELTDWFRSSPSEAPSAQPLSFEQPQQSPTFDSAFNPSASNDTPDWLKQMAANAGEK
                     DPEPASDSSFGSSDWFSQPAESNPAPQADSFAQSSDTPDWLHQMQENLDSSAQQQPSA
                     SPFAASSGDSDLPDWLNQMQAGAVPAEPAQPAEPFNLSSDTPDWLNQMRSDSTPAESA
                     PSAEPFSFSSDTPDWLSQLPADNAASQQPAADPFNASASADMPDWLSGLGSVPGGENS
                     PFVESTPAESEPFSSNSDLPSWLAPEQDAAKKTDSKPRWLQSDEEVASGEPAWLASND
                     KTLVPESEQAADETPVNDVFSDMPDWLKSAAPASTIFDDAPAFETPKAETPKQQPSSP
                     FESAPAFSEDAFPAQNDALFTEMPDWLSNAMEEPASGGMIPEPIKGGDALAPDILPSW
                     VEAMRPSDQGMSNIVNAANDATLESRGALAGLSGVLPLGAGFAPTSKPKAYSIKLNAS
                     DEQVKHAGILEQILAAETSAVSLTSEKSLGSSRVLRWSIAFVLFIVTILTSFLGTQIF
                     STPQAQPNELKLAVAVSQAIPDSAPVLVAVDYEASRAGEIEAAAAPLFDNMLLLKHPR
                     LTFVSSNESGAILAERLMNGPLAFHVQNGVSYLNLGYLSGGQMGIRAFAQNPTSVAPS
                     DVNGQPAWASPVLQNVTALNQFALIILLTDDADAARAWVEQTEDSRGAVPILVVSSAQ
                     SAPMIYPYYDSGQVAGIVSGLYGGAIFEQQYNNGRPGLARNYWDAYSIGMLLAMLLLV
                     VGGLVNLGLGVRDHNAMREGK"
BASE COUNT         1981 a         1877 c         1860 g         1677 t
ORIGIN      
        1 ctgactctat ccagaaagcg ggcaccaagc cgaatgtttt gaatctcttc ggctataccg
       61 ggcttgcatc cctcgccgcg gcggcggcgg gtgcctccgt cacccacgtg gacgcttcga
      121 agaaatccgt aagttgggcg cgtgagaatc aggaactctc cggtctgggc gatagacccg
      181 tccgctggat cgtagaagat gcgttgaagt acgtccagcg cgaagccaaa cgcggagtca
      241 aatatgatgg cgtcatcctc gacccgccca agttcgggcg cggaccgaaa ggcgaagtgt
      301 gggaagtgta caagtcgctg cccaatctgc tggatgagat ccgccagtgt ttgagcgacc
      361 atcccctctt tatgatcgtc accgtctatg ccgtgcgcgc ctccgccatt cacgtcgccc
      421 aagccgtaga agagatgatg aaaaagcata aaggcatgct cgatagcggt gaactggtaa
      481 cgcgcgagaa aagcgcaaac cgcttacttt cacaggcggt ctatgcaaga tggtcttcga
      541 aataataaaa aaacaccctg tagtacaggg tgttttttta ttttgttgat accaaactta
      601 tcgcatgggc acagcacgca atcggcgagc cattaagata acaacgataa aagccagcgc
      661 catcacagcc aaaccaggaa caccgacatc ctcaacaaaa cctgtttgag gcaaagcagt
      721 ggttgtcggc acgatggtca actgggcaac cgcagcctgc gttagtgcgg ctccaaccgt
      781 agcagtcgcc gccgctgggg tgccagcatt gacagccgct gcggcattcg ggtccgctgt
      841 agacgtagct gtagcaacat tgacaacagc agtggctgtt gggggcaaag tagcagttgg
      901 aagaatggac gcttcaaagg tagcagtcaa cgcatcattg atgaaattac tggcaacttc
      961 agcagtttga gatgcagcaa ccgcctgagc ttcaacttga gtgtttgaat tttgagtata
     1021 gagaaaaacg cctacgatac aggcaataga gagaaggata atcccaccga ggataccagc
     1081 tgcaatcagg aacgtgcggt tattattcga ctcttccggt aattgaccgt cttcgggcag
     1141 gttatcatca aaattagatt cttcaaacga catggagcac tctcctgaat taggtggtca
     1201 tagttcaacg aacgatgggg ataattatcc cacaacttcc atgtttacga gcggtacgat
     1261 tacagtctcg tcattttcca attttacttc agcagcttga gcacgcagcc cgctcggtaa
     1321 aacggtcaga ccgggcttaa tagccacaat agaaccaatc atccccatgg aaggtggacg
     1381 gcgcatgcgc acctgcatac cgggagcaaa aatttctacc tcattcggca tgggaggctc
     1441 tgcagaaatc ggcaaggcaa taaaaacttc tgggcgagca cccgtataac gatcatacgg
     1501 ctcagtattt aatgtaactt cacgtttggc atttgtgctc aacaatttat aggcggcaga
     1561 attcatcggc aaggagccaa ttccatcagt cacaaggatg gggtaacgca tctcgcgtgc
     1621 ctttggcagc aaagatggct gaatgctgga agcgatcaac ccacgggcag ggagttccgc
     1681 caaagcctgt aacgtttcaa cgttcttaat catcccgccc aaaataatcg aaccgcgcaa
     1741 gctaacatcc atacgatttg gaagtagaac ggtatctaaa ccttccatca agttgaccag
     1801 cacgcccgaa tcaatgcgac catttcccca tatgccttgg actagtccac caaaggactg
     1861 gataacaacg ccccgccccg gaacaacttg cacgacagtt cctggaatac cagctcgcac
     1921 ttccatcttg gcttcgccga tttccatcaa gacttgccca ccccctactg caaccacacg
     1981 tccatcttta ggtgcacgta cagatcgagg gaaaagtccg cgccctaccg cgatttcagc
     2041 agaagcagcc actttatcat caactttgca tttgatcagt ttatctgcaa ccgttgggga
     2101 tacgcctaag atacgagcaa catctaatag agcatgctca cgtgcccaac gtgtttctgc
     2161 caccacttca ctggcggtta ctttttgttg aactcgggca atgacattcc ctgaaatggg
     2221 caacaaacgc tcgcggacaa tggtcgtaag accaattaca tgatgtacag gtgctaacat
     2281 ggtctattcg cctcccaaag tccagttcca ctttttgatt aactctcgcc tgcgaacaga
     2341 gtcagaagga aggtcaaggg gtcttccgcg cccgtcaaag acaactccaa gtgcaccacc
     2401 tgtaacaggt aagctcttgg taccacgacc gggtccgaaa ccaatatcca cgccacgcga
     2461 gacttgaatt gtcaggtttg ccacttcacc acttgccaac ggcaggactt cgagcgagcc
     2521 gtacttcagg tttacattcg cttccgtgcc atttgcataa gccacttttg ccttcaagat
     2581 cggcgcaccg tatttggtct taaccacagg tgaaatgacc gtacctacgc ttaaaaaagc
     2641 acctgattca aggacttgca ctggcaaaat attgttttgc gatgcagcag ctcccagcat
     2701 gggcatgagg ttattttgat cgaggataac ggtagaaatg ccaacaggtt gtaccgagtc
     2761 taagagcaag agcaggctct gaccggggcg tgaagcgtcg tttagggcag cgccgccagc
     2821 aatgatgggg tcaaacaagg gagtgagagt cgggctgatg gaagcagctt cctttggaaa
     2881 atcttttcga gccgcttgca tggcaagata taaagcttgt ttcgccacag attgagcaat
     2941 ggaaaaatct tcttttgttg ccgcaatggt gtttggatac aacgcttttt gatggatgta
     3001 atcaagaatc acactagatg gaatatccaa tgtagaccag cgcaaaatat cttcaacggt
     3061 tgtgtaattc aaaagcccag tgaggttttc gccaagccca aattgtgggt ataccttcaa
     3121 gaccgattta tttgcgaaac cggcagcaat cacagaagag gatgcgccca aatctacgct
     3181 gagcaatccc ttattggagc catacacatt agagagaaag cgcaccatgc gcccggtagc
     3241 ataagcagtt ggaagcgtat gaccacccgc ccataagtcc agcacatcta accctttgag
     3301 ttgtttctta cgaatggtca caaacatttg cgccaattca agttcggctg gagcaatatc
     3361 ttccacttca agcgaaggtc ggacgtttga gctaaagtgt agcgtggttg cgagcgaccc
     3421 taccaacgat ttgacttcat caaccaactt ctcattgcct gcgaacagaa ccgccgggcg
     3481 cttttcatca ggcatcagat agctggcaag cccaatgggt tcgagcaatt tttggatgga
     3541 gcgtgaggcg cctccatccg ttccaccggc gataacaacc atgtccggac ggatgcgcaa
     3601 aatgctatcg atctgctgtt caggtttgcg ttggtcgctg aggctaaggg tgtcgaggat
     3661 acgtgcatac gtggactcgg tcagccggcg agcgctttcg agcgaaacat ctttgagcaa
     3721 gcccaccaca agcgttcgga ctgcaggacc tgcggataca gttactacaa gcgaatccac
     3781 gccggagcca tctggttggc ttggcaaaat gagactgcga gctgtcgttt cgagcaatgt
     3841 tcttttgagc acattttgaa gcgcttgaat ggcattgcga gcgccttcag aaacatcttt
     3901 atacggggct tcagccgtgc taggagccac accagaagcg acaaaacgat actcgccttc
     3961 gatcacgtcg aacaacactg cgcgggtatt cgccgcaccg acatcgatgg caagaagaga
     4021 gttactagtg accagtgaag tgggcataat acttagagca tcccaagcaa ggttttgata
     4081 aagaagacca aagacgaaac acgttcaata aaggcagtca gggctgcggc gtaaacgcca
     4141 gcaaagaccg cgcctaaggt aacgctgata aagaagcgac cgatccatgc aaacatattg
     4201 atcggggcaa aacgttttgt ggagccatct gctttggggt tcgcaccaaa atggaaatat
     4261 gccagtgagg tgacaactcc catcagcaga acggtgccat tcgcagcgac ttccaccata
     4321 ttaatgtttc gggcagcagc ggcttgaagg tcgaagaaat tagttgtggc attaacctgc
     4381 ggaaacagtg tgccggtcaa cgcccctgcg atcgtgaccg cagcgccagc tcccacaagg
     4441 aacgccatgg caattcgaga aacgcctgcc atgcccggca ctgccttcag taagataaat
     4501 acaacaccca aaaacggcac aatgagcaaa acaccttggg tgtatgcacc gcttaacaag
     4561 acctgtccaa tgggaagaaa aagcttggtg taaagcactt gccaaaacac cacagcggca
     4621 acatacccag aagcaacccc aacaaacaga taaatggcaa ctttgaaaag tggattatca
     4681 ccaatcaaat aacttaacac catgagtgta aaaacaaagc tcaaagctcc tgcaatcaaa
     4741 tcgagagaga gtactggcat cttattttcc ctccctcatg gcattatggt cacgcacacc
     4801 aagtcccaaa ttgaccaaac ctccgaccac taaaagcaac atggcgagca acatgccaat
     4861 gctgtatgca tcccaatagt ttcgcgcaag cccggggcga ccattgttgt attgttgttc
     4921 aaagatcgcg ccgccgtata gaccagagac gattcctgcc acttgcccag agtcataata
     4981 tgggtaaatc attggtgccg attgtgcgct ggacacaacc aaaattggta ccgcaccacg
     5041 tgagtcttcg gtttgttcga cccaagcacg tgcagcatca gcatcatcgg tcagtaagat
     5101 gatgagagcg aattgattta gggcggttac attctgtaaa acaggcgatg cccatgcggg
     5161 ttgaccgttg acatcagacg gcgcaacaga ggtggggttc tgcgcaaatg cacggatgcc
     5221 catctgtccg ccagagagat atcctagatt caggtaggaa acgccatttt ggacgtgaaa
     5281 agctaacggt ccgttcatga ggcgttcggc aaggatcgcc cccgactcat tgcttgaaac
     5341 aaaggtcagg cgcggatgtt tcaacaatag catattgtca aacaaaggtg ccgcagcagc
     5401 ttcaatttca cccgcacgag aagcttcata atcgacagcg accaatacag gcgcactatc
     5461 tgggatggct tgagaaactg ccaccgccaa ctttaattca ttgggctgcg cttgaggtgt
     5521 ggagaagatt tgcgtaccga ggaaactggt cagaatggtg acaatgaaaa gcacaaaagc
     5581 aattgaccag cgcaacacgc gtgaggaacc tagagatttt tcagaagtga gcgaaaccgc
     5641 agatgtttct gccgcaagga tctgttccaa gatacctgca tgcttaacct gttcatcact
     5701 cgcattcaat ttgatggaat aagcttttgg tttgctggtt ggcgcaaagc ctgcccccaa
     5761 aggcaacaca ccagaaagcc ccgccaatgc gccgcgtgat tcaagtgtgg catcgtttgc
     5821 ggcattcaca atgttagaca ttccctggtc agaaggtcgc atcgcttcca cccacgaagg
     5881 cagaatatca ggtgcgagcg catctccacc tttgatgggt tcagggatca tcccgcccga
     5941 agcgggttct tccattgcat tcgaaagcca atcaggcatt tcggtaaaga gcgcatcatt
     6001 ttgtgcaggg aaagcatctt ctgaaaatgc aggtgcagat tcaaatggag aagaaggttg
     6061 ctgtttaggg gtctctgcct tcggggtttc aaaggcagga gcatcatcaa aaattgtcga
     6121 cgcaggcgca gccgatttca accaatctgg catatcgcta aagacatcat tgaccggggt
     6181 ttcatctgca gcttgttcag attcaggaac aagagtcttg tcattagatg ccaaccaagc
     6241 gggctcccct gaagcaactt cctcgtcact ttgcaaccaa cgcggtttgg aatccgtttt
     6301 cttggctgca tcctgctcgg gagccaacca agatggtaag tcactattcg acgagaacgg
     6361 ctcagactca gccggagttg attcaacaaa cggagagttt tctccgccgg gcacagatcc
     6421 caatccgctt agccaatcgg gcatatccgc agaagctgaa gcattgaacg ggtctgcagc
     6481 aggttgctga gatgccgcat tatctgccgg aagttgactg agccaatcag gcgtatccga
     6541 agagaagcta aatggctctg ctgatggagc tgattcggca ggtgttgaat cagaccgcat
     6601 ctgattcaac caatcaggcg tatctgaaga gagattaaac ggctcagcgg gttgagcagg
     6661 ttccgcagga acagcgcctg cttgcatttg attaagccaa tccggtaaat ctgaatcacc
     6721 agatgaagct gcaaacggtg aagccgaagg ttgctgttgt gccgaagaat cgagattctc
     6781 ttgcatctgg tgcaaccaat cgggcgtatc cgaagattgg gcaaatgagt cggcttgagg
     6841 agcgggattt gattctgccg gttggctgaa ccagtctgaa gacccaaatg aagaatcgga
     6901 agctggttcc gggtcttttt ctccggcatt cgcagccatt tgttttagcc aatctggcgt
     6961 gtcgtttgat gcagaaggat taaaagcaga atcaaatgtt gggctctgtt gtggctgttc
     7021 aaaagacaat ggctgagcac ttggcgcctc tgaaggcgat gaacggaacc aatcggtcag
     7081 ttcgtctttt tcatcaggga tgggctgttg ctgcatgcct gtcaaccagc ttggaacttc
     7141 ttcttccgct tcaggttgct gaaaatcgct tttattacca gtttccaccc agcgagttcc
     7201 actagactct tctactgttt cattgggctt ttgtttaggt gcaggcgtac ctgtaatgct
     7261 ggcaagccaa tcgggaatct cttcatcgtc gtcgtcccct gcttgagatt gcagacccgc
     7321 taaaaagtcc gtgctggagg cggcaggttt ggcaggcact tctggcttgg gcggttgctg
     7381 agtctgctgt tgggt
//
LOCUS       DAOQFT010000039         7312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_4963_length_7312_cov_7.234119, whole genome shotgun sequence.
ACCESSION   DAOQFT010000039 DAOQFT010000000
VERSION     DAOQFT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4963_length_7312_cov_7.234119"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..673)
                     /locus_tag="PLR93_01800"
     CDS             complement(98..673)
                     /locus_tag="PLR93_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25718.1"
                     /translation="MANKQGSLGDRFAEITVVLVTLIALTAGWFYKSSIENASVPFEA
                     DGISAAAPKGWLQIGAEGDELLRTIDINSSGFGAVYTIRQIAITSEATSSEVASLVTL
                     DHAQNLTAFKVLNQNEIVDRNGRSAYMIDYVYVESNPDVTHAAIPSVVRGTDFVYLSS
                     DRAIVVSFQADEKNYERDLGRFYLFLESLKF"
     gene            complement(684..1541)
                     /locus_tag="PLR93_01805"
     CDS             complement(684..1541)
                     /locus_tag="PLR93_01805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNH25719.1"
                     /translation="MNESKSIHERKHVWRDEFLLVIGLCVFVGIVYALDNALHPVFTP
                     TTLLLTGVFLAIVPAFIWMIFFYLQDSAEPEPKGYVIGVFVLGGLLAASIGIPVVENL
                     FRVSHWIYTNTVATILGGILVVGFTQEFLKYAAVRYSIYHSDEYDEPTDGVIYATAAG
                     LGYATMLNINFVISNGGVDLGTGIIRMAVVALAHAAFSGITGYFLGRAKFESEQIWWM
                     PLGITLAATFNGLFNWLQGIVTRPTITLSGSTNNPWLGLALAAVVALITTGLVLTLVR
                     RNIKSLQAE"
     gene            complement(1660..2190)
                     /locus_tag="PLR93_01810"
     CDS             complement(1660..2190)
                     /locus_tag="PLR93_01810"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNH25720.1"
                     /translation="MIRPYEFGDEHELHAAIQESLPELNPWMSWATERYTLNVARDFV
                     AITRAEWSRGTLYSFAILNASTNEFLGGCGLSHFHPVYKFCNLGYWVRTSKHGNGYAG
                     CATKLVAKFAFERANAIRAEIVIAKDNHASKRVAEKVGAHYEGILLNRMTVRENIYDA
                     HMFSLLPSDFGLVAQL"
     gene            complement(2311..2967)
                     /locus_tag="PLR93_01815"
     CDS             complement(2311..2967)
                     /locus_tag="PLR93_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH25721.1"
                     /translation="MKKQRIILVDDHEVVRLGLKSLLERHPHFDVVGEAGSAREALEQ
                     TALLKPDVVVMDIRLPGTSGIEACEQIVAQFPNTKVIMLTSYAEDEMLFSAIRAGASG
                     YILKQIGSEDLIKALESVGRGEALLDPAVTQRVFQEVRRAVKEEEASAFAHLSQQEKH
                     VLLLVSEGKTNREIAKNLFLGEGTVRNYVSSILSKLNVNNRAEAAAYAVEHNLRDYIS
                     "
     gene            complement(3013..3483)
                     /locus_tag="PLR93_01820"
     CDS             complement(3013..3483)
                     /locus_tag="PLR93_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH25722.1"
                     /translation="MKELFEYREKFLARLREVTVEFCDACQAFKDPFTAVDGEWTVHQ
                     IAFHVRGIEREVYGTRVRRTLQEDNPEFKNFDPETWMADSYNRDENLETILDEFSKRM
                     NEVCDLLGDMPQEGWSRLSRHEALGKGLTLQLWAERGLAHIEEHLKALKKAQTL"
     gene            complement(3480..4067)
                     /locus_tag="PLR93_01825"
     CDS             complement(3480..4067)
                     /locus_tag="PLR93_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH25723.1"
                     /translation="MDPLKGKVVLITGAGKGIGRKLAETLAAQGAIVAANDISPINVD
                     EVVNGILAKGGQAKAYLEDVAKKVGAQMIVKSVEDEFGSLDILINHAAVEPHTPLLNM
                     DEWDWHRVLDVNLTGAFLMSQTAGRVMKEKGSGVIINLVAGAGERSKEAGAFLASKAG
                     LDELSRQLARELNPHGVKVVSASTLEEAIKALEEV"
     gene            complement(4074..4778)
                     /locus_tag="PLR93_01830"
     CDS             complement(4074..4778)
                     /locus_tag="PLR93_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25724.1"
                     /translation="MLKKIFPFLVLAVIVAAAGIYLYFTYFRGRSSARTAQVFDWFQD
                     QSSRPELMMTSGLKCGDAPFIFPTNGLIGFIWDDSFRPGHRHSGLDIFSGTEVGVTPI
                     VAAYPGYLTREADWISTVIIRVPEDPLKPNRQIWVYYTHMADPNGNSFVSSAFPQGTK
                     EVFVEAGTFLGYMGNYSGDPANPVGIHLHISVVKDDGFGNYTNELDINNTYDPSPYFG
                     MPLNANENTDVIPVCN"
     gene            4894..5277
                     /locus_tag="PLR93_01835"
     CDS             4894..5277
                     /locus_tag="PLR93_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH25725.1"
                     /translation="MTLYIQPYPFRRMVRRTVAQPQSRALSVNVREEDDAYVLYALVP
                     GLKAEDLNIQVIENVVSIEGEYKDEETSFLLSELPRGAFSRSLRMPSEIDADKVEARI
                     AEGVLTLTLPKAESAKPKKIKVNVH"
     gene            5359..6378
                     /locus_tag="PLR93_01840"
     CDS             5359..6378
                     /locus_tag="PLR93_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25726.1"
                     /translation="MRVKQTLFAILLVAILAACGGEETPDIAAPTPVPSHTPAPSATP
                     IPTLSTPLALLILPADMDQETSDLYQKTVYDLALASGFRFQVRNGITPEDLADPTLKV
                     VIALPPDPGVASYAPTAPNVQFLAVNIPNLTAGGNVSVLAPNTQVELPAFLAGYTLAM
                     MTYEYHIGMLYPEGDPNSQAALNAFSNGMRYYCGICDGIYIDPVSYPAILGVPANEDP
                     GKFGGYANVLINDNKVGGLYIYPTLATDDFLSYVGTQGVYQIGTTMPEPRPGGWLMTI
                     TPDTIKAIQTAWPQLIAGQGGQNVQSPLGIADVDTGVLTEAKLRLVQQVLDELIAGRI
                     LPSNP"
     gene            complement(6419..6844)
                     /locus_tag="PLR93_01845"
     CDS             complement(6419..6844)
                     /locus_tag="PLR93_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25727.1"
                     /translation="MTVSNTSTQNSILIISQDVDMTRVWKNLFEQKNCRVISETTARD
                     GVHAARTLSPTLIILDLDMPDKDRLDLCRDLRASTNGTLLLINASHKQVDVLEYHRAG
                     VDETVSDSISPMALLVKSLAWLARQDWIVPRKQSAQLWA"
BASE COUNT         1884 a         1862 c         1706 g         1860 t
ORIGIN      
        1 gagacgctgc cttacatgcc aggctgacga tggcaaggat ggtgaagaac atccaaagat
       61 atttattgaa gtttggtttc atgctcgcct cgctacacta aaacttgagc gattcaagga
      121 agagatagaa ccttccgaga tcgcgttcat aatttttctc atctgcctga aaagaaacca
      181 cgatcgcgcg gtcactactc aggtacacaa aatctgtgcc gcgtaccacg ctcggaatgg
      241 cagcatgagt cacatcggga tttgattcga catacacata atcgatcatg tacgcactgc
      301 gcccgtttcg atctacaatt tcgttttggt tcaacacttt gaacgcagtt aaattctgag
      361 cgtgatccaa agtgacaaga ctcgccactt cggaacttgt cgcttcggat gtaatcgcga
      421 tctgacgaat cgtgtagaca gcaccgaaac cgcttgagtt gatgtctatg gtacgaagca
      481 gttcatcgcc ctctgcaccg atttgcaacc agcctttggg cgctgcggca ctgattccat
      541 ctgcctcaaa cgggactgag gcattttcaa tgctcgattt atagaaccat cccgctgtga
      601 gtgcgatcag ggttacaaga acaacggtga tctccgcaaa gcggtcaccg agagaacctt
      661 gtttgtttgc catgtctgtt ctcctactca gcctgcagcg atttaatgtt gcggcgcacc
      721 aaggtgagca caagcccggt ggtgattaac gcaacaactg ccgcaagcgc caagcccagc
      781 caaggattat tggtcgagcc gctcaaagtg atggtcggtc tggtgacgat accttgcaac
      841 caattgaata agccgttgaa agtggcagcc agagtgatgc cgagaggcat ccaccaaatt
      901 tgttcagatt cgaacttggc acgcccgagg aagtagcccg taatgcccga gaatgccgca
      961 tgcgcaaggg caacaactgc catacgaatg atgcctgtgc ccaagtccac gccaccgttg
     1021 gagatgacaa agttgatgtt gagcatggtc gcatagccca acccggcagc tgtggcgtag
     1081 atcacaccgt cagtcggttc atcgtattcg tcggaatgat agatgctata ccgaacggca
     1141 gcatacttta gaaactcttg tgtgaagccc actacaagaa ttccgcctag aatggtggca
     1201 acggtattgg tatagatcca atgagaaacg cggaagaggt tttctacaac aggaataccg
     1261 atggacgccg ctaaaagccc gcccaatacg aagacgccga tcacatagcc tttcggttca
     1321 ggttcggcgc tatcctgcag atagaagaaa atcatccaaa taaatgcagg gacaatggca
     1381 agaaagacac ctgtaagcag caaggtggtg ggggtaaaca cggggtgtag ggcattgtcc
     1441 agtgcataga cgatgccaac gaagacacac aagccgataa cgagcaggaa ctcgtcacgc
     1501 caaacgtgtt tccgttcatg aattgatttg gattcattca tggaattctc tctccttatt
     1561 aagtgatcca tcaaataaca accccatcat agtaaaaaga tgggtgtacg tcaatagagc
     1621 tgggttacaa atgggggaca gactgagcgg agaaaatgct tacagttggg caaccagccc
     1681 aaagtctgag gggagaagcg agaacatatg cgcgtcataa atattctcgc ggacggtcat
     1741 gcgattcagc aaaatccctt cgtaatgcgc tcccactttt tctgctacac gtttgctggc
     1801 gtggttatcc tttgcaatta cgatctctgc acgaatcgca ttggcgcgtt cgaaggcaaa
     1861 ctttgctaca agttttgtgg cacaaccggc gtatccattg ccatgcttgg atgtccgcac
     1921 ccaatagccg aggttacaaa acttatagac cgggtgaaaa tggcttaagc cacaaccgcc
     1981 aagaaattcg tttgtgcttg cattcagaat ggcaaaagaa tataaggtgc cgcgtgacca
     2041 ttctgcacgc gtgatggcga caaaatcgcg cgcgacattg agcgtgtatc gttcggttgc
     2101 ccaactcatc catggattta actccggtag cgattcttga atcgcagcat gcagttcgtg
     2161 ttcatccccg aactcatagg ggcgtatcat cacaacatca tcggtcaatt ttacaaaagg
     2221 gaacatagtt ttttaggcgg gaataaaaaa agagacactt cttcacgcgg ggtgcgaaag
     2281 tgtctctttc atagtggctg agaaaacgaa ttaggaaatg taatctctca aattgtgttc
     2341 caccgcgtag gctgctgctt cagcgcggtt gttcacattt aacttcgaga gtatgctcga
     2401 aacataattg cggaccgttc cctcaccgag gaagaggttc ttggcgatct cgcgattggt
     2461 cttgccttca gagacgagta gaagcacgtg tttttcttgc tggcttaagt gtgcaaaggc
     2521 tgaagcttct tcttccttca cggcgcggcg cacttcttgg aagacgcgct gggtcacagc
     2581 agggtctagt agcgcttcac cacgtcccac tgattcgagg gctttgatca aatcttcgct
     2641 gccaatttgt ttgagaatgt agcccgaagc acccgcacga atggcagaaa agagcatttc
     2701 atcttctgca tacgaagtga gcatgatgac ttttgtgttg ggaaattgcg caacaatctg
     2761 ttcgcaagct tcaatgccag aggttcccgg caggcgaatg tccatcacca ccacatcagg
     2821 tttgaggaga gccgtctgtt ccaacgcttc acgcgccgac ccagcttcac ccacaacatc
     2881 aaaatggggg tgtctctcta aaagcgactt cagtccgagc cgcacaactt catgatcgtc
     2941 aaccaagatg attctttgtt tcttcataat aggcactatt ttaccgcagg aaattggaaa
     3001 aacgggcaaa aattataggg tttgtgcttt tttcaaagct ttcaaatgtt cttcaatgtg
     3061 tgccaacccg cgttctgccc acaactgcaa agttagaccc ttgcccaagg cttcgtgtcg
     3121 gctcaaacgt gaccaacctt cttgtggcat gtcacccaat agatcgcaga cttcattcat
     3181 gcgtttcgaa aattcatcta agatagtttc aaggttctcg tcacgattat aagaatccgc
     3241 catccatgtt tcagggtcaa aatttttgaa ctcgggattg tcttcttgca aggtgcggcg
     3301 gacgcgtgtg ccatacacct cacgttcaat gccacgcaca tgaaaagcga tctgatgcac
     3361 cgtccattcg ccatcaacgg ctgtaaatgg gtctttgaat gcctggcatg catcacaaaa
     3421 ctcaaccgtc acttcgcgca ggcgtgctaa aaatttctcg cgatattcga acagttcttt
     3481 catacttcct ccaatgcctt gatagcttct tccaaagtcg aagcagagac caccttcaca
     3541 ccatgcggat ttaattctcg tgctaactgg cgggacaatt catccaagcc ggctttgctg
     3601 gcaagaaacg ctcccgcctc tttcgacctt tcgcctgctc ctgccaccaa gttgataatc
     3661 acgccggagc ctttttcttt catcacccgc cccgccgttt gagacattag aaaagcgccc
     3721 gttagattta catccaacac gcggtgccaa tcccattcat ccatattgag caaaggggta
     3781 tgcggttcca ccgctgcatg gttgatcaat atatcaagcg acccaaattc atcttccacg
     3841 cttttgacga tcatttgcgc accgaccttt ttggcaacat cttccaaata agcctttgcc
     3901 tgccctccct ttgcgaggat tccgttcacc acctcgtcca cattaattgg ggagatgtca
     3961 ttggctgcca caattgcacc ttgagccgcc aaagtttctg ccaatttgcg accaattccc
     4021 ttgcctgctc cggtaattaa aaccactttg cctttcaatg gatccatcct ttcttaatta
     4081 cacactggta ttacatctgt gttttcattt gcattcaacg gcatcccaaa ataggggctt
     4141 gggtcgtagg tgttgttaat gtccaactcg tttgtgtaat tcccgaagcc atcatcttta
     4201 accaccgaaa tatgcaagtg gattcccact gggtttgccg ggtcaccgga ataattaccc
     4261 atatacccaa ggaatgtccc tgcttcaaca aagacttctt tggtgccttg tggaaaggca
     4321 gaagagacaa aagaatttcc atttgggtct gccatatggg tgtaatacac ccatatctgg
     4381 cgattgggct tcaacgggtc ttctggcaca cggataatta ccgtagaaat ccaatctgct
     4441 tcgcgagtga gataacctgg gtaggcagcc acaatgggtg ttacgccaac ctccgtccca
     4501 ctgaaaatgt ctaacccgct gtggcggtga ccggggcgga atgaatcgtc ccaaatgaag
     4561 ccgatcaagc cattggtggg gaagatgaaa ggagcatcac cacatttcaa gccgctggtc
     4621 atcatcagct cagggcgaga ggactggtct tggaaccagt caaatacttg agcggttcgc
     4681 gcagaagacc tgccgcgaaa ataggtgaag tacaagtaaa tccctgccgc agcgacaata
     4741 acggcaagga ccagaaacgg aaatatcttt ttcaacattc aaaagattat accgccctga
     4801 actttcttat aaaaaatcta ccaagtatta gcatttttct tacattgcag atttaatatt
     4861 gcgtcattga cataaacaaa aggagacata aacatgaccc tctatatcca accttatccg
     4921 ttccgccgta tggttcgccg taccgtcgct caacctcagt cccgcgctct tagtgttaac
     4981 gtccgcgaag aagatgatgc atatgtcctt tatgcgctcg tccccggttt gaaagctgaa
     5041 gacctgaaca ttcaggtgat cgaaaatgtt gtcagcatcg aaggcgaata taaggatgaa
     5101 gaaacatcct tccttctgag cgagcttcca cgcggagcct tcagccgcag cttgcgaatg
     5161 ccgtctgaaa ttgatgcaga caaggtcgaa gccagaatcg cagaaggcgt cctaacgctg
     5221 acactgccca aggcagaatc agccaaaccg aagaagatca aagtcaacgt tcactaatca
     5281 agccgcaggt cacgcacaat gcgtgacctg tttttttccc gttataatgt tccggacatt
     5341 ttgtaacgga gaaattcagt gcgcgtaaaa caaactcttt ttgcaattct tctcgtcgca
     5401 atcctagccg cttgtggtgg ggaagagacg ccggatatcg cagccccgac gcccgtcccg
     5461 tctcatactc cggcgccatc tgcgacgccc atcccaacct tatccacgcc gttggcattg
     5521 ctcatcctcc ccgccgatat ggaccaggag acttcggact tataccaaaa aacggtgtat
     5581 gacctggcat tggcttctgg cttccgcttc caggttcgca atggaatcac tcccgaagac
     5641 ctcgcggacc caaccctgaa agtagtcatt gctttgcctc ccgaccctgg tgtggcaagt
     5701 tatgcgccga ctgcaccgaa tgtgcaattc cttgctgtaa atatccccaa tctcactgcg
     5761 ggcggaaatg ttagcgttct tgccccgaac acccaagtag agttacctgc ttttctggca
     5821 ggctatactt tggcgatgat gacctatgaa tatcatattg gtatgctcta cccagaaggt
     5881 gacccgaact cacaggctgc cttgaacgcc ttcagcaacg gcatgcgtta ttactgtggc
     5941 atctgtgatg gtatttatat cgaccctgta agttacccag ctatcttggg tgtacctgcc
     6001 aatgaagacc ccggtaaatt tggtgggtat gccaatgtat tgatcaacga taacaaagtg
     6061 ggtggcttat acatttaccc cactcttgct acggatgatt ttctctctta tgtaggtacc
     6121 cagggtgttt atcagatcgg gacgaccatg ccagaacctc gccccggcgg ctggctaatg
     6181 accattacgc ccgatactat caaagccatt caaactgctt ggccccagtt gattgccggg
     6241 caaggcggac agaacgttca gtctccactt ggtatcgcag atgtggacac tggcgttctt
     6301 acagaagcaa agttacgtct tgtgcagcaa gtattagatg agttgatcgc aggtagaatt
     6361 cttccttcca acccatagaa ttgagaaaaa aagacccgcg aatttcgcgg gtcttttttt
     6421 atgcccaaag ttgcgccgat tgtttgcgcg gaacgatcca atcttgccgt gccagccatg
     6481 ccagtgattt gaccaataat gccattgggc tgatcgagtc cgatacggtt tcatccactc
     6541 cggcgcggtg atattcgagt acatccactt gtttgtggct ggcattgatg agcagcagtg
     6601 tgccattggt gctggcacgc aagtcgcggc aaaggtctag ccggtcttta tctggcatat
     6661 ccaagtcaag aataatcagg gtaggggaga gagtgcgtgc cgcgtgtact ccatctcgcg
     6721 ctgtggtttc gctaatgacg cggcaatttt tctgttcgaa taagttcttc caaacacggg
     6781 tcatgtctac atcttgtgag atgatcagga tactgttctg agttgaagta ttgctaactg
     6841 tcatggctac ctctctataa acactaggtc gttgaaaact ttgaaaaggg tcttaattca
     6901 cctaaatgtc ctacttgact ggatttgttc gtgcgtgata aactcgcgtt acctgaaaaa
     6961 ggaattctaa agaaaggagc gcactaccca tgttcatgat gcaattcttc atttccgcag
     7021 atgccagctt cgctggtgat agcagtagtg cgcctgttcg tcgttaggtt taactgaaac
     7081 cttcgcgacc acggcgcgtc aagcccgtgg tcgtttttct ttaaccgcct tagaacggtc
     7141 acgggagaga acctcttgtg accgtttttt gttatttagt aaagaaggaa atcatgttta
     7201 cgattgaaac ccagttgctt gaagcacagg acgtacgttt aggtcccata gaccttgaaa
     7261 cgcatcccgc tgtcgaatcc cgctggacgc acgacgccga atttatgcac tt
//
LOCUS       DAOQFT010000040         7184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5082_length_7184_cov_5.954973, whole genome shotgun sequence.
ACCESSION   DAOQFT010000040 DAOQFT010000000
VERSION     DAOQFT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5082_length_7184_cov_5.954973"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1162
                     /locus_tag="PLR93_01850"
     CDS             143..1162
                     /locus_tag="PLR93_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25728.1"
                     /translation="MNSLNRMKTKRPAWWILFLIVFVFACNLPSASTPTPDLFATLSA
                     VTPAPADSTSQPNPSTSSTAEPTGKIVFVCQIFKTQASNQICIINADGSGFRRLTTDN
                     TRQHYYPSLSPDGKSVVYAAFREPNIYEIYEMDIATGNVAQLTDRLGNLNGPEISPDG
                     SMIAFKLSTAAANQIWLMNRDGSDPHPISNAFGWDPTWYPDGTSILFASDMQGVVQLY
                     VIKLDGSELQKISDLPAIRGRSDWSPNGQFIVTYSGQPWNRDVYIMNADGSDARMLTP
                     TGGNSQGPSISPDGQWVVFTSYYDHYGEDHGCEIYIMSVNDTQQVVRRLTDNDYCDYQ
                     PRWGP"
     gene            1179..2006
                     /locus_tag="PLR93_01855"
     CDS             1179..2006
                     /locus_tag="PLR93_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH25729.1"
                     /translation="MDIKGKVVIITGASSGIGAAAALEFGREGAKVVLAARRTDKLQE
                     LVNEIRAMQNGAEAFVLQTDISKLDDIQRLVAQTVERFGRVDVLVNNAGFGRLKWLET
                     LDPLEDIQAQFDVNVLGVIQTTRQVLPIMIKQRSGSIINICSIAGLVGTPTYTIYSAT
                     KHAVHGFSEALRREVKPWGIDVSLIYPGGVRGTEFPDHAGIQRKTNASTPKFMVLTAE
                     QVGKAVVSLVHRPRRMWVLPWMWRFTIFLNRFLPGLVDRTTIKNFTIPERADELNKK"
     gene            complement(2030..3742)
                     /gene="recJ"
                     /locus_tag="PLR93_01860"
     CDS             complement(2030..3742)
                     /gene="recJ"
                     /locus_tag="PLR93_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNH25730.1"
                     /translation="MTQDKLWNISPRISPEADSALAAFPPLLRQVLFNRGFADDPSAR
                     AYLRGEPNSNTNPFQITDMQVAIERIRYALANNEPIAIYGDYDVDGVTATALLVETLK
                     KLGADVRGYIPNRFDEGYGLNNNALDELKANGVKLVITVDCGIRSPAEAAHAQTIGLD
                     LIISDHHHPDDDNLPPALAVINPKRHGDIYPDKDLAGVGIAYKIAEALLTKTDQADVV
                     NGQILNSLLDLVALGTVADLAPLVGENRVLVKKGLKQMRQTTRQGLFSLAAVADISLA
                     KVNAGNIGFGLGPRLNAAGRLKEALAAFELLTTTDVFRAGELAQQLDMQNRERQRITR
                     DMQTRAEEIALNDDPNAHLLFAAHEEFNSGVVGLAASRLTEKYYRPAIVASKGEEETR
                     GSCRSIPEFHITDALDQCADLLVRHGGHAAAAGFTVRNENIEALVSKLKAIAEEKLAG
                     SDLKPTVTADAEVDLSEIRPEVYEKGLKYLEPTGYGNREASFVARNVKVKSSRIVGSD
                     GKHLKMSLEDSKGIAHDAIGFRLGDWHKSMPPRVDVLFTYEPNEYNGRVTYQLNLKDL
                     KASR"
     gene            3818..4333
                     /locus_tag="PLR93_01865"
     CDS             3818..4333
                     /locus_tag="PLR93_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25731.1"
                     /translation="MRALLPDEKLSTVFIYTPTMLVRGDLILRENIRASIWLRTQGVP
                     NYIHLYAAQVIQLAGSPPKNYTSQETFIPTAEVIGFHLAPPAQDPLDYDATEANRKME
                     SVHVLAGSFEMKAKLRISTSADFASGLDVMNTSWLSLYEAEITNPYLPQLKLTVPMLL
                     ARPSKLTISLL"
     gene            4366..4770
                     /locus_tag="PLR93_01870"
     CDS             4366..4770
                     /locus_tag="PLR93_01870"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNH25732.1"
                     /translation="MEELTLTTPALLFPTVSLLMLAYTNRFLTLATIIRNLNDRYHDS
                     QDKNLLAQIDNLRYRVYLIRNMQIFGVLSLLMCVVSMFALFFNWTDGGVWSFGIALVL
                     MIISLGISLRELQISVGALDLLLTNLEEHETK"
     gene            4798..5634
                     /locus_tag="PLR93_01875"
     CDS             4798..5634
                     /locus_tag="PLR93_01875"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNH25733.1"
                     /translation="MDTDKLIQTMVDEIKTVSGVKAIVLGGSRARGTYTPSSDIDLGI
                     YYDPNQPMDLTALGKIATQLDDEHRTDVITGFGGWGPWINGGGWLRVQSLPVDFLYKD
                     LGRVKSYIDACLNGKVEIFYQGGHPHGFVTSIYLGEVALCKPLYDPEGIIAELKGKVT
                     PYPEALQKAILASFAWEIDFSVGIAKKSIERADVNYAAGCCFRAVMCMLQVLFALNKE
                     YWMNEKGAINITDRFAIKPADFRKRVAEVYALLEASPEALEKAIYLLKGLSEDVYGLL
                     PK"
     gene            complement(5692..7119)
                     /locus_tag="PLR93_01880"
     CDS             complement(5692..7119)
                     /locus_tag="PLR93_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNH25734.1"
                     /translation="MKTLLIPLDTNATKALYLQVADYITEAARSGQLKPGTQLPPSRT
                     LAEQNNVHRSTIINAYQELKARGVLASKQGSGTYIEKGLSENTVSIQKFKPESLPTDE
                     LIAEIRRMHWSEGMISLGYGPPSDELMPVEDFDRSRQRVLRRDGKKTANYEHPQGYYP
                     LRQAICADLVKQGIQADPDEILITNGVLEGVALVSRAMAAPGDAVLTELPQYFGNWTN
                     FSYLGLNVRGFDLLESGPDFASLEAQLQDENDRPRFVFVTADHQNPLGTCWTMPARHE
                     FLKMMNKYDVPVVEDATYRDLTYDGMPHTPLRALDPDVIYVGTFSHSLMPGLRIGFVL
                     CRGRLREHIERLKAISSGPVETLNQRALADFLATGEYERHLDRIIPVYRARRDALLNA
                     MQTYFPPELRWTKPTGGFFVWIWLPDPISAIDLFYRSVKKGISIAPANIFYPGPCPQN
                     AFRLAFSHYPEDVLTRAVMEIGKLL"
BASE COUNT         1732 a         1673 c         1883 g         1896 t
ORIGIN      
        1 cctcctgtgg gttgggtcag tcccgggtag gctgcaccga gcggcgcggt gacgcaccgc
       61 ccagagagat gattgcccaa atggcgcaag tcattggaca gaatgcggct tatagaccag
      121 cctgaagcga tttttttatt ggatgaattc tttaaatcgg atgaagacca aacgccctgc
      181 atggtggata ctattcttaa tcgttttcgt ctttgcttgc aatttaccct ccgcttctac
      241 cccaacccca gacctgtttg ccactctatc agcagttacc cccgccccag cagactctac
      301 ctctcagcct aacccatcaa cctcatccac agcggagcca actggcaaga tcgttttcgt
      361 ttgtcagata ttcaaaacac aagccagtaa tcagatttgc atcatcaatg ccgatggctc
      421 tggttttcgc cgtttgacca ccgataacac tcgtcaacat tattacccat cgctatcgcc
      481 agatggcaaa agtgttgttt atgcagcatt tcgtgaaccg aatatttacg aaatctacga
      541 aatggatatt gccactggca acgttgctca actaaccgac cgtctcggca atctcaacgg
      601 accggaaatt tcgcctgatg gttcaatgat cgcgttcaaa ctctccaccg cggcagccaa
      661 tcagatttgg ttgatgaacc gtgatgggag cgacccgcat cccatttcca atgcttttgg
      721 ctgggaccca acttggtatc cagatggaac ctccattttg tttgcctctg atatgcaagg
      781 tgtcgttcag ctctatgtga taaagctcga tggaagcgaa ctgcaaaaga tcagcgacct
      841 gcccgcgata cggggaagaa gcgattggtc accgaatgga cagttcatcg tcacatattc
      901 gggtcagcct tggaatcgcg atgtttacat catgaatgca gatggttcgg atgcccgcat
      961 gctcacacca acgggcggta actcgcaagg tccatccatt tcaccagatg ggcagtgggt
     1021 ggtgtttacc tcttattatg accattatgg cgaagatcat ggatgtgaaa tctacatcat
     1081 gtctgtcaat gacacacagc aagttgtacg ccgactaaca gacaacgatt attgtgatta
     1141 ccaaccccgc tggggacctt aagaacaaca aggacaacat ggacatcaaa ggaaaagtag
     1201 taatcatcac cggcgcttcg tcggggattg gggcagcggc tgcgcttgaa tttgggcgtg
     1261 aaggggcgaa ggttgtgtta gctgcacggc gaactgacaa gttacaagaa ttggtgaatg
     1321 agattcgtgc aatgcagaat ggcgcagaag cctttgtact tcaaaccgat atctcaaaac
     1381 tggacgacat tcaacgtttg gttgctcaaa ccgtggagag atttggtcgt gtggatgtgt
     1441 tggtgaataa tgcaggcttt ggacgtttga aatggctcga aacgctcgac ccacttgaag
     1501 atattcaagc acaatttgac gtgaatgttt tgggcgttat tcaaaccaca cgacaagttt
     1561 tgccgatcat gatcaaacag cgcagtggga gtattatcaa catctgctcc attgctggtt
     1621 tggtgggcac gccgacctat acaatttact ctgctaccaa acatgccgtg catggctttt
     1681 cagaagcttt gcggcgcgaa gtcaaacctt ggggaattga cgtctctctc atctatcccg
     1741 gcggcgtgcg tggaacagaa ttccccgacc atgccggtat tcagcgcaag accaacgctt
     1801 ccacgccaaa gttcatggta ttaacggcgg aacaagtagg caaagctgtg gtgagccttg
     1861 tgcaccgccc gcgccgaatg tgggtattgc cttggatgtg gcgttttact atttttctca
     1921 atcgtttttt gccagggcta gtggaccgta ccacgatcaa aaacttcact attccagagc
     1981 gtgctgatga actgaataaa aaataaacag ccgaaaggct gtctattttt tatcttgaag
     2041 ctttcaagtc tttcaaattc aattgatagg tgacgcgccc gttgtattca tttggctcat
     2101 atgtgaaaag cacatccacc cgtggcggca tggacttgtg ccaatcgcca aggcgaaagc
     2161 cgatcgcatc gtgagcaatg cctttggaat cttccagtga catcttcaaa tgcttgccat
     2221 cagaacccac aatgcgcgaa cttttgactt tgacattgcg tgccacaaac gacgcttcac
     2281 gattgccata cccggttggt tcgagatact tcaagccttt ctcgtacact tctgggcgta
     2341 tttcggataa gtccacttcc gcatctgccg taacggtggg cttgaggtca cttccagcga
     2401 gcttttcttc ggcgatagct tttagttttg agacgagtgc ttcaatgttt tcatttctaa
     2461 ctgtaaaccc tgccgccgcg gcatgaccac catgccgcac gagcaagtct gcgcattgat
     2521 cgagcgcatc ggtgatgtga aattctggga tggaacggca ggagccacgc gtttcttctt
     2581 cgcctttcga agcgacaatg gcgggacggt aatatttttc ggtgaggcgt gaggcagcga
     2641 gacctacaac ccctgagttg aactcttcat gagcggcaaa gagtagatgt gcatttgggt
     2701 catcattcaa tgcgatctct tcggcacggg tttgcatatc gcgcgtaatg cgttgacgtt
     2761 cacggttttg catgtctaat tgctgggcga gttcgccggc gcggaagaca tctgttgtgg
     2821 tgagtaactc aaacgccgct aacgcctctt ttaaacgacc cgccgcgttc aagcgtggac
     2881 caagcccaaa gccgatattc ccggcattta ctttcgctaa cgaaatatct gccacagccg
     2941 ccaatgagaa caaaccctgg cgagtggtct ggcgcatttg cttaagtcct tttttgacga
     3001 gtacgcgatt ttcaccaacc agcggagcga ggtctgcgac ggtgccgaga gcgacgagat
     3061 cgagcaagga gttgagaatt tgcccattga ccacatccgc ttggtctgtc ttggtgagca
     3121 aggcttctgc gatcttgtat gcaatgccta cgccggcgag gtctttatcg ggatatatat
     3181 cgccatgacg tttcgggttg atgacggcaa gcgcgggcgg caggttatcg tcgtcggggt
     3241 ggtggtggtc gctgataatg agatccagcc caatggtctg cgcatgagcc gcttcggcgg
     3301 gcgaacggat gccgcagtct acggtgatga ccagcttgac tccatttgct ttcaattcat
     3361 ccaacgcatt gttgttgaga ccgtagcctt catcaaagcg attgggaata tagccacgca
     3421 catcagcacc gagttttttg agcgtttcca ccaataatgc ggtagcagtg actccgtcaa
     3481 catcgtagtc accataaatg gcaatgggtt cgttatttgc aagcgcatag cggatgcgct
     3541 ctattgcgac ttgcatatcg gtgatttgaa aaggattggt attgctgttg ggttcgccgc
     3601 gcaaataggc acgtgcgctg gggtcatcgg caaagccgcg attgaaaagc acctgacgta
     3661 acagcggagg aaaggctgcc aaagctgaat cagcttcggg ggagatgcga ggcgagatgt
     3721 tccaaagttt gtcttgtgtc atgttaaagt gtgcggagta taaacgagac caaagtcaag
     3781 aggaagggcg cgagtataat ctgttgagga gcgacgcatg agagctttat tgcctgatga
     3841 aaaattatcg actgtgttta tttatacgcc taccatgttg gtgcgtggag atttgatctt
     3901 gcgagaaaat atccgcgcaa gtatttggtt acgcacgcag ggtgtgccaa attacattca
     3961 cttgtatgcg gcgcaggtga ttcaattggc aggttcaccg cccaagaatt acaccagcca
     4021 ggaaactttt attcctactg cggaggtgat cggttttcat cttgcgcctc ctgcgcaaga
     4081 ccctttggat tatgacgcga cggaagcaaa tcgtaaaatg gagtctgtgc acgtgttggc
     4141 tggttctttt gaaatgaaag ccaagttgcg catctctacc tccgccgatt ttgcctccgg
     4201 tttggatgtg atgaatacat cctggctttc tctgtatgaa gcagagatca caaatccgta
     4261 tttgccgcaa ttgaagttga cagtgcctat gttgttggca cgccccagta aactgacgat
     4321 tagtttgtta tagaatgtgc ggcatatgcc gcttgagtat ttactatgga agaactgacc
     4381 cttaccaccc cagcactttt gtttcctacg gtgtcgctac tgatgttggc gtacacgaat
     4441 cgttttttga cccttgccac gatcatccgt aatttgaatg accgctatca tgatagccag
     4501 gataagaacc ttctggcgca gatcgataac cttcgttatc gcgtgtatct cattcgaaat
     4561 atgcagatct ttggcgtttt gagcctgttg atgtgtgttg tcagcatgtt cgctttgttc
     4621 tttaattgga cggatggtgg tgtgtggagt tttggcatcg ctttggtgtt gatgattatc
     4681 tcgttgggaa tttctttgcg tgaattacaa atttcagtag gcgctttgga tttgctgttg
     4741 acaaatttgg aagaacacga gacgaaataa agaatgtctc tgaaagtgta ggtggttgtg
     4801 gatacggata aacttattca aaccatggtc gatgaaatca aaacggtttc aggcgtaaaa
     4861 gccattgtgt taggcggctc gcgtgccaga gggacgtata ccccttcttc tgacattgac
     4921 ttgggaattt actatgaccc aaatcagccc atggatttga ccgctttggg taagatagcg
     4981 actcagcttg acgacgaaca ccgaacggac gtcattaccg gcttcggcgg ttggggtccg
     5041 tggatcaatg gtggcgggtg gttgagagtt caatcgttgc cggtagactt tttgtataaa
     5101 gatttgggga gagttaaatc ctatattgat gcttgtttaa atggcaaagt tgaaattttt
     5161 taccaaggcg ggcatccgca tggatttgtg acctcaattt atttggggga agttgcgctt
     5221 tgcaaaccct tatatgaccc agaaggtatc attgcagaat tgaagggcaa agtcacgccg
     5281 tacccggagg cgttgcaaaa ggcgattctt gccagttttg cttgggagat cgatttttcc
     5341 gtgggaattg ccaagaaaag catcgaacga gcggacgtaa attatgcggc gggttgttgc
     5401 ttccgtgcag tgatgtgcat gttgcaggtt ttgtttgcgt tgaacaaaga atactggatg
     5461 aacgaaaaag gcgctatcaa catcacagac cgttttgcca tcaaacccgc agatttccgt
     5521 aagcgtgttg cggaagttta tgcgttatta gaagcctctc ccgaagcgct agagaaagca
     5581 atctatttgt tgaagggttt gagtgaggat gtctacgggc tgttgccgaa gtaaagttga
     5641 attcaaaaag acctcggagg tttttaaaac ctccgaggtc ttttgtttta attacaacaa
     5701 cttcccaatc tccattactg cccttgtcaa cacatcttca gggtaatgag agaatgccaa
     5761 acgaaatgca ttttgcgggc agggaccggg gtagaagatg ttcgcggggg cgatgctgat
     5821 tccttttttc acggaacgat agaacaagtc aattgcagaa atagggtcag gtaaccagat
     5881 ccacacgaag aagccgccag taggtttcgt ccagcggagt tctggcggga agtaggtttg
     5941 catcgcgttc agaagcgcat cgcgtcgtgc gcggtagacg gggataatgc gatcaagatg
     6001 acgttcatat tcacccgtag caagaaaatc tgccagcgcg cgttggttaa gcgtttcgac
     6061 gggtccgctg gagatggctt tgaggcgttc aatgtgttcg cgcagacgcc cacggcaaag
     6121 cacgaatccg atacggagac cgggcatcag cgaatgagaa aacgtcccga catagatcac
     6181 gtccggatcc agagcccgaa gcggtgtgtg gggcatgcca tcgtaagtca agtcgcgata
     6241 ggtcgcatcc tctacgacag gcacatcgta tttgttcatc atcttgagaa attcgtggcg
     6301 ggcgggcatc gtccaacaag tgccaagcgg attttgatga tcggctgtca cgaagacgaa
     6361 tcgtggtctg tcattttcat cttgaagctg cgcctcaagc gacgcaaaat cgggtccgct
     6421 ttcgagcagg tcaaagccac gcacattcaa accgaggtac gaaaaattag tccaattgcc
     6481 aaagtattgt ggcagttcag ttaacacggc atcgcccggt gccgccatgg ctcggctcac
     6541 aagtgcaacg ccttccaaga ctccatttgt gatgaggatt tcatctgggt ctgcttgaat
     6601 accctgcttg acgaggtctg cgcagatggc ttggcgcagt ggataatacc cttgcggatg
     6661 ttcgtagttg gctgtctttt ttccatcgcg cctcaacacc cgctgacgcg aacggtcaaa
     6721 gtcttccacg ggcatcagct catctgacgg cggaccatac ccaagtgaga tcatgccttc
     6781 agaccaatgc atacggcgaa tttctgcgat cagttcgtcg gtgggcaggc tttctggctt
     6841 gaacttttga atcgacacgg tgttctcgct caatcccttt tcgatgtaag tcccgcttcc
     6901 ttgtttggat gccagcacac cacgcgcttt gagttcctga taggcattga taatagttga
     6961 gcggtggacg ttattctgct cagccaaggt gcgcgaaggc ggtaactgtg tgccgggctt
     7021 taactgtccg ctgcgggcag cttcggtgat gtaatctgca acttgcaggt aaagcgcttt
     7081 ggtggcgttc gtatctaaag ggatgagcaa tgttttcacg ggttggattc taccagtacg
     7141 tatggaacgg tcactggcaa tccgcgttcg ttgatttcag gtag
//
LOCUS       DAOQFT010000041         7183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5083_length_7183_cov_6.380892, whole genome shotgun sequence.
ACCESSION   DAOQFT010000041 DAOQFT010000000
VERSION     DAOQFT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5083_length_7183_cov_6.380892"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..962)
                     /locus_tag="PLR93_01885"
     CDS             complement(123..962)
                     /locus_tag="PLR93_01885"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNH25735.1"
                     /translation="MKHQEFNWKAKDGLDIFAQVWEPDVVLPRAVVCLVHGLGEHSSR
                     YAHVAEAFSKEGYILFSFDHRGHGRSGGIRGHISSIEDFLQDIDQLLSQARTRYPNLP
                     VILYGHSLGGILVLNYSLNRKPDIKGVIATSSGLHTALEEQPVKIFMAKLLGSLMPKT
                     TLPSGLDAKAISRDETVVQTYNNDPLVHDKISLGFGKIMLGVTQWTLEHASEFPLPLL
                     LLHGKSDTIAYPSSSTEFAAALKDRATLVLWDGAYHELHNEPEKAEVFKTMLMWMDAR
                     LRG"
     gene            complement(965..1573)
                     /locus_tag="PLR93_01890"
     CDS             complement(965..1573)
                     /locus_tag="PLR93_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25736.1"
                     /translation="MIPARLSPRLMNRFVETLSKEVGADTYHAVLSNSGLSRDWQKPE
                     FFLAFDNKQTAEAYAKLQSALRTYYGRGARGILLRIGSKLWEPMLNDASFASKAQATL
                     IRGFPKPLKRKPALELLASTLGAGRGDVTVHTLDLDLLLVDHSSPTTLGQKENSPVCF
                     VTLGLIRECLYWATGDEHDIEELVCKAAGSHQCEFKITIGAS"
     gene            complement(1573..2436)
                     /locus_tag="PLR93_01895"
     CDS             complement(1573..2436)
                     /locus_tag="PLR93_01895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25737.1"
                     /translation="MINLHLHIRRAVAQDHRQLSNLLFQESNTHRHLDWRTALEWIGV
                     HNFWVLEDSGTVVAALACPEDPPDIAWIRLFSFSPHLSGPEAWSDLWNFASAEIRAIN
                     PRAEAASIVMKQWFQTLLLDSGFEPRLSIVLLELKSEDYVPVRISDAIRMRPMQEADL
                     GIVEDVDREAFGKFWHNTSDALQRAHGQSFYSSVAEDANGIIGYQISTGNPFGVHLAR
                     LGVRPEAQGKGVGSALVHDLIQRAGGLQSGRLSVNTQDDNVASLRLYSKLGFLRTGEH
                     YPVFIHSLGAS"
     gene            complement(2433..2954)
                     /locus_tag="PLR93_01900"
     CDS             complement(2433..2954)
                     /locus_tag="PLR93_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25738.1"
                     /translation="MSLEPHQLDAKGKQAFAKKNFVEAAEYFRQAAEGYTQGRAGLEA
                     AESRNNLSVALLQAGKPQEALDAALDTDKVFEGAKDVKRQAMAFGNQAAALEGLHRYD
                     EAIQKYELSAELFGSIDEGDLRAMVMKSAAAIKLKTGKITDSAFKMMGSLDVKKNPTF
                     FERIMKFLMKFIR"
     gene            complement(2960..3634)
                     /locus_tag="PLR93_01905"
     CDS             complement(2960..3634)
                     /locus_tag="PLR93_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH25739.1"
                     /translation="MTNAQITIREKKLGLLIRDARVAERRSIKECADAIGVKAGVFRA
                     YEEGRRAPSLPELEALVFFLKIPISQFWGNETISDAPEPLKPEDITKLIALRHRMIGA
                     LIRQERTNANMSIRHLSSETGIPQSRLKAYELGERTVSVPELESILVVMGSRIENFFD
                     QSGPVGEWMTTQLAMQKFMDLPNEFQEFVCQPVNRPYLELAMKLSSMSRDKLRSVAEG
                     LLDITL"
     gene            complement(3674..4993)
                     /locus_tag="PLR93_01910"
     CDS             complement(3674..4993)
                     /locus_tag="PLR93_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNH25740.1"
                     /translation="MNTPLSGAPQRLTGQDLIRLKKYLADNLLRALETEGIEASQRVT
                     YIQQNIGRVFDTTQLKFPEDLKREIFKQVLNDLLGFGPIQPLLDDPDISEIMVNGPKK
                     IFIEKKGQLTKSEITFDDDDHVLRVIDRIIMPLGRRVDFDSPTVDARLPDGSRVNAVV
                     RPVSIDGPSITIRKFRKDKLKAEDLIAFGSITKQMADFLEACVKARFNIVISGGTGSG
                     KTTLLNVVSGFIPEGERIVTIEDAAELQLQQDHVMRMETKPANSDGLHAVTIRDLVKN
                     SLRMRPDRIVVGEVRGGEALDMLQAMNTGHDGSLTTVHSNSPRDAISRLETLVLMAGM
                     ELPLKVVRTQISSAIDLIVQQTRLKDGKRKVTAITEVAGMEGDVVVLTDIFKFNQTGV
                     TQDGQVLGELQPTGIRPNFTPRLEAAGFKLGAEVFMTSKQQQQSSRR"
     gene            complement(4990..5283)
                     /locus_tag="PLR93_01915"
     CDS             complement(4990..5283)
                     /locus_tag="PLR93_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25741.1"
                     /translation="MSIEYDDKGKYYTNVIQKVPVPAMVQTTTNLIRGIVHVRHGERL
                     KDEIEGSEQFVAMTDVSVYSADGKELYTGPFLALQKGQIVWVMPLNETQKGEE"
     gene            complement(5316..6464)
                     /locus_tag="PLR93_01920"
     CDS             complement(5316..6464)
                     /locus_tag="PLR93_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25742.1"
                     /translation="MSQKKILIVDSDVASRNFIARKLQELDYETLQAGSGKEGLIYTW
                     RDHPDLVIADPALTDIKGEEFATKLRNDPRTANLPIVALSSDHNPARIKSCQDAGFND
                     YITKSGQAVPMLKDIINRLFGVTQEDMKQGGLMIVFLSAKGGTGTSSVCANTAVNITK
                     DQPEARIAVVDLVFPIGSIAQIVGYEGTDNIVTVADRNPLETDVDFLKTLPRIEPWNF
                     NLLAGSPDPELSNQLNVGRIWDIITGLKSSYDYVLIDIGRALSKITLPLIQHADLISL
                     LISTDVSTISLTKTILDFLKSKGVKPEAIYTILNRHASLEGLSKREAETILEKNVNTA
                     IPYLGTNFGFANSQHRPFTLKFPNDTASIIFHDSAREMANLAKKIRSQ"
     gene            complement(6503..>7183)
                     /locus_tag="PLR93_01925"
     CDS             complement(6503..>7183)
                     /locus_tag="PLR93_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH25743.1"
                     /translation="LIVDDEPRYLRLLEANLRTEGYEVVTAQDGLQALDVFSSNPIDL
                     VLMDVMMPRLDGFGATQRLREFTNVPIVILTARGEEQDRVRGLDLGADDYLIKPFSAT
                     ELLARVRAVLRRAHNPTDAGQVRFFTHDDLKIDFARAEVWRGEEAVSLSATEYRLLLQ
                     FAHNVGKILTSEELLTSVWGVEYKNDKEILWVSIARLRQKLEKDAHTPRHIVTRSGLG
                     YLMPPIET"
BASE COUNT         1923 a         1717 c         1625 g         1918 t
ORIGIN      
        1 cgtagttggc gtttactcat ctctttcgtc atgctagctc ctattatttt tttatcggca
       61 ttgcattttc tcatgccaaa atagatttgt ccagaaatgg gttaacaggt tcttattttc
      121 gatcagcctc gcaggcgagc gtccatccac atcagcatgg tcttgaaaac ttctgccttt
      181 tccggctcgt tatgaagttc atgataagca ccatcccaaa gaacaagggt ggcacggtct
      241 ttcagagcag cggcaaactc agtgctgctg gaaggatacg caatagtatc gctcttaccg
      301 tgtagcaata acaacggaag tgggaactcg ctagcatgct ccaaagtcca ttgagtgacc
      361 ccaagcataa tcttcccaaa cccgagagag attttgtcat gaacaagcgg gtcattattg
      421 taggtttgca caactgtttc atcacgagag atggcttttg catccaaacc actaggaagg
      481 gttgtcttgg gcatcagaga gccaagtaat tttgccataa atatctttac aggttgttct
      541 tccaaagcgg tatgaagccc agaactggta gcaatgacgc ccttaatatc cggcttgcga
      601 ttcaaactgt agttcaagac taggatacca cctaagctat gcccatacag aataacaggc
      661 aggttcgggt atcgagtcct tgcttgagat aataactgat ctatatcttg taggaagtct
      721 tcaatagaag agatatgccc acgtataccg ccagagcgac catgtccgcg gtgatcgaag
      781 gagaagagaa tgtatccttc tttgctaaat gcttcggcaa catgagcata ccgtgaacta
      841 tgttcaccca agccgtgcac taagcacacc acggcacggg gtaacaccac atctggctcc
      901 caaacctggg cgaaaatatc aagcccgtct tttgctttcc aattaaattc ttgatgtttc
      961 atgtctatga tgctccaatc gtgatcttaa attcacattg atgagacccc gccgctttac
     1021 agacaagttc ttcaatgtca tgttcatcgc cagttgccca atataaacat tcacggatca
     1081 agcccagggt tacaaaacac acaggggaat tttctttctg tcccaacgtg gtaggagaag
     1141 aatggtcaac cagaagcagg tcaagatcca aagtatgaac cgtgacatca ccgcgtcccg
     1201 caccaagggt tgaagcaagg agttcaagcg caggtttacg cttcaatggt ttgggaaacc
     1261 cgcgtatgag agttgcctga gctttagagg caaaagaagc atcgttcaac atgggttccc
     1321 aaagcttaga accgattctc aaaaggatac cgcgtgcgcc gcgcccataa taggtgcgca
     1381 aagctgattg caatttggca tatgcttctg cagtttgttt attatcgaag gcaagaaaaa
     1441 attctggctt ctgccaatct cgtgacaaac cagaatttga taaaactgca tgataggtat
     1501 cggcgcccac ttcttttgaa agagtttcta cgaagcgatt cattaggcgt ggagaaagtc
     1561 gggcaggtat cactatgacg ctcccaagga atggataaaa acagggtagt gctctcctgt
     1621 gcgaagaaag ccgagtttcg aatacaaacg caatgacgcg acattatcat cctgcgtatt
     1681 gacagatagt ctaccagact gaagcccgcc tgctctttgg atcaaatcat gtacgagggc
     1741 tgagccaact cccttgcctt gcgcctcagg gcggacccca agccttgcaa ggtgcacccc
     1801 aaacggattc cccgtactga tctgatatcc aattattcca ttggcatctt cagcgacaga
     1861 ggagtagaaa gattgaccat gtgcacgttg gagagcatct gaagtattgt gccagaattt
     1921 tccaaaggct tcacggtcta cgtcttcaac aataccaagg tctgcctctt gcatcggtcg
     1981 cattcgaata gcatcactga ttcgaacagg aacataatct tcacttttga gttcaagaag
     2041 aacaatactt aagcgtggtt caaacccact atctaaaagc agcgtttgaa accactgttt
     2101 cattacaatc gaagctgctt cagcacgcgg gttgatagcc cgaatctcag cggaggcaaa
     2161 gttccatagg tcagaccaag cttcgggtcc agaaagatgc gggctaaatg aaaatagacg
     2221 aatccatgcg atatctggcg ggtcttcagg gcaagccaag gcagccacca cagtccccga
     2281 atcttccaat acccaaaaat tatggacgcc aatccactca agggctgtgc gccaatcgag
     2341 atggcggtgt gtatttgatt cttgaaatag gagattggaa agctgacgat ggtcctgcgc
     2401 aacggcgcgc cggatatgca ggtggagatt gatcatctga taaatttcat gagaaacttc
     2461 atgatacgtt caaaaaaggt tggattcttt tttacgtcga gcgagcccat cattttgaaa
     2521 gcagaatcgg tgatcttgcc tgttttgagt ttgatggcag ccgcagattt catgaccatt
     2581 gcgcgcaggt caccttcatc aatactgcca aacaattctg cagagagttc gtatttttga
     2641 atcgcttcat catatcgatg aagaccttcg agcgccgcag cttggttacc aaacgccatg
     2701 gcttgacgtt tcacatcttt ggcaccttca aagactttat cggtatcaag agctgcatcg
     2761 agtgcttcct gaggtttgcc tgcttgcaag agtgcaacac ttaagttgtt acgagactct
     2821 gctgcttcaa gtccggcacg tccttgggtg tacccttcag ctgcctggcg aaaatattca
     2881 gcagcttcaa caaagttctt tttggcaaag gcttgtttcc ctttggcgtc gagttgatga
     2941 ggttcaagag acataattat taaagggtga tgtcgagcaa gccttctgca acagagcgaa
     3001 gtttatcgcg agacatgctg ctaagcttca tggcgagttc aagatacggg cggttcacag
     3061 gctggcaaac aaattcttga aattcattgg ggaggtccat aaatttttgc atggcaagct
     3121 gagttgtcat ccactcgccc acaggaccgc tttggtcgaa gaaattttct atacggctgc
     3181 ccataacaac aaggatgctt tccagctcgg gcacagaaac cgtacgttcg cccaattcat
     3241 atgccttcaa tcgtgactgt ggaattccag tctcggatga aagatgacga atggacatat
     3301 tcgcattcgt gcgctcctga cggatcagcg cgccgatcat gcgatggcgc aaagcaataa
     3361 gtttggtaat atcttcaggt tttaagggct cgggagcatc tgaaatagtt tcattgcccc
     3421 aaaattgcga aatgggaatc tttaaaaaga atacaagagc ttccagttca ggcaaagagg
     3481 gtgcgcgccg tccttcttca taggcacgga acacacctgc ttttacgcca atcgcatcgg
     3541 cgcactcctt gatactgcga cgttctgcca cacgggcatc tcgaatcaac aaaccaagtt
     3601 tcttttcgcg aatagtaatt tgagcgttgg tcatgttacc tcatcaaata agcccttatt
     3661 atagcagaat gcgtcaacgt cttgacgact gttgctgttg tttagacgtc ataaacacct
     3721 ctgcgcccaa cttgaaacca gccgcctcta accttggggt gaagttgggg cgaatacccg
     3781 taggctgtaa ctcgcccaaa acttgaccgt cttgcgttac accggtttgg ttaaatttga
     3841 aaatatcagt tagaacgaca acatcgcctt ccataccagc gacttcagtg attgctgtta
     3901 ccttacgttt gccgtcctta agacgcgtct gctgaacaat aaggtcaatt gccgatgaaa
     3961 tctgagtgcg aacgacctta agcggcaatt ccatacccgc cattaaaacc agtgtctcta
     4021 gacgggaaat tgcatcacgc gggctattcg aatggacagt ggtcaatgaa ccatcgtgcc
     4081 ccgtgttcat agcctgaagc atatcaagcg cttccccacc gcggacttcc cccactacga
     4141 tacggtctgg gcgcatacgc aaagagttct taacaaggtc gcggatagtc actgcgtgca
     4201 acccgtcgga gttggcgggt ttcgtctcca tacgcatgac atggtcttgt tgcagttgaa
     4261 gctcagcagc gtcttctatg gtcacaattc gctcgccttc gggaataaag ccagaaacca
     4321 cattcaacag agtggtcttc cctgaacctg ttccgccaga aatcacaata ttaaatcttg
     4381 ctttcacaca ggcttcgaga aaatctgcca tttgttttgt aatggatcca aatgcaatta
     4441 agtcctcagc cttcagttta tctttgcgaa acttacggat tgtgattgaa ggtccatcaa
     4501 tggagacagg tcgaactaca gcattgacgc gggaaccatc aggtaaacgt gcatcgaccg
     4561 taggtgagtc aaaatccacg cggcgaccca atggcataat gattcggtca ataacgcgca
     4621 atacatgatc gtcatcatca aaggtgatct cgctcttggt gagttgcccc tttttctcga
     4681 tgaaaatctt tttaggtcca ttcaccatga tttcagaaat atctgggtcg tccaaaagtg
     4741 gttggatagg accgaagccc aacaggtcat tcagaacctg cttaaaaatt tctcgtttta
     4801 ggtcttcagg gaatttgagc tgtgtagtat caaatacacg cccgatgttt tgctgaatat
     4861 atgtcactcg ctgcgatgct tcgatccctt ctgtttcgag tgcacgtaaa agattatctg
     4921 ccagatactt cttcaggcga ataaggtcct gcccagttaa ccgttgtgga gcgcctgaaa
     4981 gtggtgtgtt cattcttccc ctttttgtgt ctcattcaac ggcatgaccc aaacgatctg
     5041 ccctttttgt agagccaaga atggacctgt gtacagctct ttcccatccg cgctataaac
     5101 actgacatcc gtcatggcaa caaattgctc gctgccttct atctcatcct tcaaacgttc
     5161 accatgccgt acatgaacta ttccgcggat gagatttgtt gtggtctgta ccatggctgg
     5221 cacaggcact ttttgaataa catttgtgta gtactttcct ttatcgtcat attctataga
     5281 catgggcttt atctccctct gcccgggaaa gacacctatt ggctacggat cttttttgcg
     5341 aggtttgcca tttccctggc agaatcatga aaaattatag aggctgtatc gttagggaat
     5401 tttaatgtaa aagggcggtg ttggctgttt gcaaatccga aatttgtacc gaggtaggga
     5461 attgcagtat ttacattttt ctccaaaatc gtttcagctt ctcgtttgct tagaccttcc
     5521 aaactagcat ggcgatttaa gatggtatag attgcttctg gctttacccc tttcgatttg
     5581 agaaaatcca atatggtttt ggtcaacgag atggtgctga catctgtgct aattaacaaa
     5641 gaaatcaagt cagcatgttg aattaaaggc agtgtaatct ttgagagagc gcgcccaatg
     5701 tcaattagga cataatcata ggaagatttc agcccagtaa taatatccca aatgcgcccc
     5761 acattcagtt ggttactcag ttcagggtct ggggaacctg ccaagaggtt gaaattccaa
     5821 ggctcgattc tgggcaaagt cttaagaaaa tccacatcag tttccaatgg gtttcggtct
     5881 gcaacagtga caatattatc cgttccttcg tagcctacta tctgagcaat ggacccgata
     5941 ggaaatacca aatccaccac ggcaattcgt gcttcgggtt gatccttcgt aatattgacc
     6001 gctgtattgg cacaaactga agaagtacct gtgccgcctt tggcgctgag aaaaacgatc
     6061 atcaaaccac cctgtttcat atcttcttgt gtaaccccaa acaagcgatt gatgatgtct
     6121 ttcaacatcg gtaccgcttg tcccgatttc gtaatgtaat cgttgaagcc cgcatcctgg
     6181 caagatttga tacgagcagg gttatggtcg ctacttaaag cgacaatagg caaattagca
     6241 gttcgcgggt cgttgcgtag tttagtagca aactcttccc ccttgatatc ggtcaaagca
     6301 gggtcagcaa tcacaaggtc tgggtggtcg cgccatgtat aaatcagacc ttccttacca
     6361 gaccctgcct gtaaggtttc ataatcaagt tcttgtaatt ttcgtgcaat aaagtttcgg
     6421 cttgcgacat ctgagtctac gatcaaaatt ttcttttgag acatatcttg ttcctgagcg
     6481 caacccgttt tatgcgcaag tattacgttt caataggggg catcagatac cctagcccag
     6541 aacgggtaac aatgtgcctg ggggtgtgag catctttttc aagtttctgg cgtaaacggg
     6601 cgatgctcac ccataaaatt tccttatcat ttttatattc aacaccccaa acactggtca
     6661 gcaattcttc agaagtgaga atcttgccaa cgttatgagc gaattgaagc agtagtctat
     6721 attcagtggc agagagagaa accgcctctt caccacgcca cacttcggca cgggcaaagt
     6781 ctatcttcag gtcatcatgt gtaaagaagc gcacttgccc tgcatcggtt gggttgtgag
     6841 cgcggcgcaa tacagcacga acacgagcaa gcaactcagt tgcagagaaa ggcttgatca
     6901 ggtaatcatc tgctccaagg tctagcccac gaacacgatc ttgttcttcg ccgcgtgccg
     6961 tcagaataac aataggcaca ttggtgaatt cacgcaaacg ctgggttgca ccaaagccat
     7021 ccagcctggg catcatcaca tccatcaaca caaggtcgat ggggttggat gaaaagacat
     7081 ccagcgcttg taagccgtct tgggcggtta ctacctcata tccttctgta cgaaggtttg
     7141 cctctaacag acgaagatag cggggttcat catctacgat cag
//
LOCUS       DAOQFT010000042         7173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5096_length_7173_cov_7.684181, whole genome shotgun sequence.
ACCESSION   DAOQFT010000042 DAOQFT010000000
VERSION     DAOQFT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5096_length_7173_cov_7.684181"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..167
                     /locus_tag="PLR93_01930"
     CDS             6..167
                     /locus_tag="PLR93_01930"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNH25744.1"
                     /translation="MDSDHGTEAITRVLIDTRNTTSRWSTVGAGTNIIDASWRALADS
                     IEYGLMVAH"
     gene            254..1312
                     /gene="leuB"
                     /locus_tag="PLR93_01935"
     CDS             254..1312
                     /gene="leuB"
                     /locus_tag="PLR93_01935"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNH25745.1"
                     /translation="MNFKITLLPGDGIGPEVVGEAVRVLETIASKYKHSFSFQERLMG
                     GCSIDKYGSSLTDETLADCVASDSVLLGAVGGPKWDDPKAKDRPERGLLALRKGLGVF
                     ANLRPVKVHPALIDGSPLKPEKLNGVDILVIRELTGGLYFGFPKGRDIKDGRERAVDT
                     LEYYDYEIKRIMNLAFNLAKGRKKKVTSVDKANVLESSRLWRQIATQVGKENPDVELE
                     HVLVDTASMRLITGPAWMDVVVTENMFGDILTDEASVLAGSMGMLPSASLGESTVGLY
                     EPIHGSAPDIAGKGIANPIGTILSTAMMLRHSFKLEEEAASIEKAVDTAITDGIRSAD
                     LGGTLTTRQLADEIIKRI"
     gene            1332..2246
                     /locus_tag="PLR93_01940"
     CDS             1332..2246
                     /locus_tag="PLR93_01940"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNH25746.1"
                     /translation="MESKFIWKNGELVPFEQATLHFLTPALHYGAAVFEGIRTYNTPK
                     GPAIFRLREHVERLFDSARVLGFREFPWSFDEAIKAHKDTVRANGFKDCYVRPLIYLD
                     GGGWNLNVDYGKPSLMIAVWEWNNYLGDDALVNGIHANISSFTRHHVNVTMTKAKIAG
                     NYANSILAKTESERNGFQEAIMLDPQGFVAECTGENLFVVRDGVIYTPSTAPVLEGIT
                     RSSLATIAKDLGYEVKEAPISRDQLYIADEVFVCGTAAEVVGLSKIDYRQIGDGKTGP
                     ITRKIQETYHDAIRGKVAKYESWCDLVG"
     gene            2451..3311
                     /gene="rpsB"
                     /locus_tag="PLR93_01945"
     CDS             2451..3311
                     /gene="rpsB"
                     /locus_tag="PLR93_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNH25747.1"
                     /translation="MANISMKALLESGVHFGHRTNKWDPRMKPYIFTERNGIHILDLQ
                     QTVKLANNAYQVIRDTVANGGTVLFVGTKRQAQETVMEEAVRAGMPYVTERWMGGMLT
                     NWSTMYQRIQELERLEKLRDSGDINRLTKKEGLLIEREITRLQTRLSGVRAMKRVPDL
                     LFIVDVGREDAAVAEANHLNIPIVALVDTNCNPQNIDYVIPSNDDAIRAIKLLVSKMA
                     DAALEGKASRKDEEPEQAAEAQAEAKPKGRPARKVVETDDQVGDDALLGKSTLAKMAK
                     TDEGTETAAE"
     gene            3354..3959
                     /gene="tsf"
                     /locus_tag="PLR93_01950"
     CDS             3354..3959
                     /gene="tsf"
                     /locus_tag="PLR93_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNH25748.1"
                     /translation="MEITTEMIKQLRGATNAPMLDCRKALQEADGDFDKAVDWLREKG
                     MATAAKRADRDASNGTVELYSHGGGRVGVMVEINCETDFVARNEQFRTLAHEIALQIA
                     ASAPKYIVADEIPAAELEHEAEIARARAKEEGKPDSVLPKIVEGRIEKYKDEVCLLRQ
                     SYIRDESITIEKLILQNVAAIGENIIVRRFQRWELGESINK"
     gene            4020..4736
                     /gene="pyrH"
                     /locus_tag="PLR93_01955"
     CDS             4020..4736
                     /gene="pyrH"
                     /locus_tag="PLR93_01955"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNH25749.1"
                     /translation="MAELKYKRILLKLSGEALGGSSGFGIDVNEAESIASRIKEVYDM
                     GVDVAVVIGAGNLWRGKQGLERGMDRSTADYMGMLATVMNAMALMDALERQGVFTRVL
                     SAIEMRAIAEPYIRRRAVRHLEKRRVVIFGAGTGNPFFSTDTAAALRATEIDAQVVIK
                     ATKVDGVYDSDPKKNPDAKKFDQLSYIDVLNRRLEVMDSTAITLCMENKIPILVVNLW
                     DSKALTSALYGEKVGTLVSG"
     gene            4911..5480
                     /gene="frr"
                     /locus_tag="PLR93_01960"
     CDS             4911..5480
                     /gene="frr"
                     /locus_tag="PLR93_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNH25750.1"
                     /translation="MSTDEVKSLLKDAETRMHGAIQSLVDDLNGIRTGRASPALVERL
                     PVEYYGTPTPLQNLASISVPEPRTLTIKPFDAGSLKDIEKAIRSSDLGLNPNSDGKVI
                     HLNLPPLTEERRRDLVKHMSHRLEESRVAVRNVRRDLHNDIREFEKEKLITEDDLKRG
                     EDDLQKLTDKFIEEIARLGQNKEKEIMEV"
     gene            5524..6270
                     /locus_tag="PLR93_01965"
     CDS             5524..6270
                     /locus_tag="PLR93_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNH25751.1"
                     /translation="MTDTPLNIPLEKVPQHVAMIMDGNGRWAIQRGLPRLAGHKAGTE
                     NLRRVIRATVEFGVKYLTIYAFSTENWGRPAEEVNGLMLILQNVIDRELNELHNEGVQ
                     LRHIGRLERLDPAIQKKVLHAIDLTKNNDRLVLNVAFNYGGRDEIVNAIQNIIKDGIP
                     AEEVTDEMVNRYLFTAGVPDPDLIIRTSGELRVSNFLIWQAAYSEWYITPTFWPDFDK
                     EEYHRALATYANRDRRYGKVSSGELQESNA"
     gene            6263..7075
                     /locus_tag="PLR93_01970"
     CDS             6263..7075
                     /locus_tag="PLR93_01970"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNH25752.1"
                     /translation="MRRRLITALGLAVVGVPAILFGGFLYYLLMGTFLIGAAWEYVHL
                     FRAVKFDPNMIVTVGGVAAVTLTRMFLPQFAQPTLAIVVLLAMAVHLIAYERGRDQSA
                     LDFGVTIGGIVYIGWIGSYLLDLRNLPEGGWWFMLVMFCVWAGDSGAYSIGRAYGNHK
                     MAPRLSPKKSWEGYAASVFTGAHTGALYVWIFQTFGHHIYSITIWQGALMGLLLGALP
                     TLGDLGESMIKRQSGIKDSSDIIPGHGGFFDRIDSWLWGSVIGFYFLTWFIL"
BASE COUNT         1802 a         1782 c         1852 g         1737 t
ORIGIN      
        1 gcattctgga ttcagatcac ggcaccgaag ccattacccg tgtgttgatc gacacccgca
       61 ataccacgtc tcgctggagt acggtcggtg ctggcacgaa tatcatcgat gccagttggc
      121 gtgccttggc tgactcgatt gaatatgggt taatggtggc gcattaagta cgggcgggga
      181 gacctcgccc aagattgtta ataaagaaac tgggcgcgga gactgcgccc ctacaagaat
      241 ggaaaacata tagatgaact tcaaaatcac cttactcccc ggcgacggca tcggacctga
      301 agtggtcggt gaagccgtgc gggtgctgga gacgatagcc agcaaatata agcactcatt
      361 ttcatttcaa gaacgattga tgggtggttg ctccattgac aaatacggct catccctgac
      421 cgatgagact ctcgcagact gcgtcgcttc agactccgtt ttgctgggtg ccgtcggtgg
      481 accgaaatgg gatgacccga aagccaaaga ccgccccgag cgcggcttgc tcgcattacg
      541 caaagggctt ggcgtatttg ctaatttacg ccctgttaaa gtccacccgg cgttgatcga
      601 tggttcaccg ctcaaaccag aaaagctcaa tggcgtggat atcctcgtca ttcgtgagtt
      661 gaccggcggg ttgtactttg gcttcccaaa gggtcgtgat attaaagatg gtcgcgaacg
      721 tgcggtggat actctcgaat attacgacta cgagatcaag cgcatcatga acctcgcctt
      781 caatttagcg aagggacgca agaagaaggt cacttcggtg gataaagcca atgtgcttga
      841 atcctctcgc ttgtggcgtc aaattgcaac tcaggtgggg aaggagaatc ctgacgttga
      901 actggagcac gttttggtgg atacggcttc gatgcgcctc atcactggtc ccgcgtggat
      961 ggatgtggtc gtcaccgaga acatgttcgg tgatatcctc acggatgaag cgtctgtctt
     1021 ggcagggtcg atggggatgt tgccctctgc cagtttgggc gaatcaactg tcggcttgta
     1081 tgagccgatc cacggatccg ctccagatat tgcaggcaaa ggcatcgcaa atccgattgg
     1141 cacgattctt tctacggcga tgatgttacg ccactcgttc aagttggaag aagaagctgc
     1201 atctattgaa aaagcagttg acactgcgat cacagacggg atccgttctg cggacctcgg
     1261 cggtacatta actacccgtc agttggcgga tgaaatcatc aaacgaatat agaggagaat
     1321 gagttatggg catggaatcc aaatttattt ggaaaaatgg ggagctggtg ccgtttgaac
     1381 aggccacctt gcattttttg acacccgcgt tgcattatgg agctgccgtc tttgaaggga
     1441 ttcgtacgta taacacgccc aagggaccgg caatcttccg cttgcgtgag catgtggagc
     1501 gcctgttcga ctctgcgcgc gtgttgggct tccgtgaatt tccgtggagc ttcgacgaag
     1561 ccattaaagc gcacaaagac actgtccgcg cgaatggatt caaagattgc tatgtgcgtc
     1621 cgttgattta cttggatggc ggtggctgga acttgaatgt ggactatggc aagccatctt
     1681 tgatgatcgc ggtttgggaa tggaacaatt acctcggcga cgatgctctt gtgaacggta
     1741 ttcacgccaa tatttcttcc tttactcgtc atcatgtcaa tgtgactatg accaaagcca
     1801 agatcgctgg taattatgca aatagtattc ttgccaagac cgaatcagaa cgaaatggtt
     1861 ttcaagaagc gattatgctt gaccctcaag gctttgttgc agaatgcaca ggcgaaaacc
     1921 tttttgtagt tcgtgatggt gtgatctata ctccgtccac cgcaccggtg ttggaaggta
     1981 tcacacgttc atcacttgca acaattgcca aagaccttgg atatgaagta aaggaagctc
     2041 caatttctcg cgaccagctc tatattgcag acgaagtctt tgtgtgtggt acagccgcgg
     2101 aagttgttgg cttgagcaaa attgactacc gccaaattgg cgatggcaag accggtccta
     2161 tcacccgcaa aattcaagaa acgtatcacg atgcaattcg cggcaaggtg gcaaagtacg
     2221 aatcttggtg tgaccttgta gggtagggga acgctcatat cagcatttga cgatggacga
     2281 caagtgtcgt ccatcgtttg attcttgtga tataatcccg ccgccttgaa aaaggcataa
     2341 tctgcctggg acgggcaaaa gcgtccctga gcgggtcaac atccccgcta aactcattcc
     2401 gctccacacc accacgagat gcgtgtcgga gagcggattt taggagaaac atggctaata
     2461 tttccatgaa agccctgctt gaaagcggcg tccatttcgg tcaccgcacc aacaagtggg
     2521 atccccgtat gaagccgtac attttcactg aacgcaacgg cattcatatc ctcgaccttc
     2581 aacagaccgt caagttggca aacaatgcct atcaggtcat ccgcgatacc gtcgccaatg
     2641 gcggaaccgt gctcttcgtt ggcaccaagc gccaggcaca ggaaactgtg atggaagaag
     2701 ctgtccgcgc gggcatgccg tacgtcaccg aacgctggat gggtggtatg ctcaccaact
     2761 ggtctactat gtatcagcgt attcaggaac tcgaacgcct cgaaaaactg cgcgatagcg
     2821 gcgatatcaa ccgcctcacc aagaaggaag gcttgttgat cgaacgtgag atcacccgcc
     2881 tccagacccg cctcagcggt gttcgcgcca tgaagcgcgt gcccgacctg ctctttatcg
     2941 tcgacgtggg tcgcgaagat gcagccgtgg cagaagccaa tcacctcaac atcccgatcg
     3001 tggctttggt cgataccaac tgcaatcctc aaaacattga ctatgtcatc ccttccaatg
     3061 atgacgccat ccgcgccatc aagttgcttg tctccaagat ggcagatgct gctctcgaag
     3121 gcaaagcctc tcgcaaggac gaagaacccg aacaagctgc tgaagctcag gctgaagcca
     3181 agcccaaggg acgccctgct cgcaaagtgg ttgagaccga tgaccaggtg ggtgatgatg
     3241 ccctcttggg taagtccact ctcgccaaga tggcaaagac cgacgaaggc actgaaaccg
     3301 ctgctgaata agctgctagt tgaataacac agaaagcaac aaggatagac acgatggaaa
     3361 ttacgactga gatgatcaag caactgcgcg gcgcaaccaa cgctcccatg ttggattgcc
     3421 gcaaggcgct tcaggaagct gatggcgatt ttgataaggc tgtagactgg ctccgcgaaa
     3481 agggcatggc gaccgctgcc aagcgcgccg accgcgatgc ctccaacggc accgttgaac
     3541 tgtattccca cgggggcgga cgcgtgggcg tgatggttga aatcaactgc gaaaccgact
     3601 tcgttgctcg caatgaacag ttccgcaccc ttgcccatga aattgctctt cagatcgctg
     3661 ctagcgctcc gaaatacatt gttgcggatg aaattcccgc cgccgaactc gaacacgaag
     3721 ccgagatcgc ccgtgctcgt gccaaagaag aaggcaaacc cgatagcgtt ctccccaaga
     3781 tcgtggaagg ccgcatcgaa aagtacaaag acgaagtctg ccttttgcgc cagtcataca
     3841 tccgcgatga atcgatcacc attgaaaaac tcattctcca gaatgtggct gctattggtg
     3901 aaaatatcat cgtccgacgc ttccagcgtt gggaacttgg cgaaagcatc aacaaatagt
     3961 ttcttaaaaa gccagccttc gggttggctt tttttctaat tcgatttgga ggtatgagaa
     4021 tggctgaatt gaaatacaag agaatcctgc tcaagttaag tggcgaagcg cttggcggtt
     4081 catccggatt tggcattgat gttaacgaag cagagtcaat cgccagccgc atcaaagaag
     4141 tctacgacat gggcgtagat gtagcagttg tgatcggcgc tgggaacctt tggcgcggca
     4201 agcaagggct tgaacgcggc atggaccgct ccacagcaga ctatatgggc atgcttgcca
     4261 ccgtgatgaa tgccatggcg ttgatggatg ccctcgaacg acagggcgtg tttacccgcg
     4321 tcctctctgc cattgaaatg agagccatcg ccgaacctta tattcgccga cgcgctgttc
     4381 gccacctcga aaaacgccgt gtcgtcattt ttggcgctgg aacgggaaac cccttcttct
     4441 caaccgatac cgctgcggcg ttacgcgcta ctgaaatcga tgcgcaggtt gtcatcaaag
     4501 ccaccaaagt ggacggggtg tatgactcag acccgaagaa aaaccccgat gcgaaaaagt
     4561 ttgaccaact cagctatatt gacgttctta accgccgtct cgaagtgatg gatagcactg
     4621 ccatcactct gtgtatggaa aacaaaattc cgatccttgt ggtcaacttg tgggattcaa
     4681 aagcgcttac cagcgccctg tatggcgaaa aagtgggcac tttggtctct gggtaattct
     4741 ttttataccc aagcctctct ctttccaacg gaaggggaga ggcttttctg ttccaatagt
     4801 tgatagtctc tctatatttt tcataaacta tcaatgtttt taccatccca tagcctaaat
     4861 tcttgtttta aggtatcctt gtattgtaat tattttcttg gaggaagatc gtgagtactg
     4921 acgaagtaaa gagtttgtta aaagacgccg aaacccgcat gcacggcgcg atccaatccc
     4981 ttgttgacga cctgaatggc atacgtactg ggcgcgctag cccggcattg gtggaaagac
     5041 ttccggtcga atactatggc accccaaccc cgcttcaaaa cttagcttcc attagtgtgc
     5101 ccgaaccacg cacattgacc attaagcctt ttgatgccgg ctctctcaag gatattgaaa
     5161 aagcgattcg ctcctcagac cttgggctga atcccaactc cgatggtaag gtcattcact
     5221 tgaacctgcc gccccttaca gaagaacgcc gccgcgactt ggttaagcac atgagccacc
     5281 gtcttgaaga gtctcgggtg gcagttcgca atgtgcgccg tgatctgcat aacgatatcc
     5341 gcgaatttga gaaggaaaag cttatcactg aagacgacct caaacgcggt gaagacgacc
     5401 ttcaaaagct taccgataaa ttcatcgaag agatcgccag acttggtcaa aacaaagaaa
     5461 aagagatcat ggaagtatag ccatcccctt tgatgccttt tggcattgaa aaggtcgatc
     5521 catatgacag ataccccttt gaacattccg cttgaaaaag tcccgcaaca tgttgccatg
     5581 attatggatg gcaacggacg ttgggcaatt caacgtggac ttccccgcct cgctgggcat
     5641 aaagcaggaa ctgaaaatct gcgtcgcgtc atccgcgcaa cggtcgaatt tggtgtgaaa
     5701 tacctcacca tttacgcgtt ctccaccgaa aattgggggc gccctgctga ggaagtgaat
     5761 gggttaatgc tcatccttca aaatgtgatt gatcgggaat tgaacgaact tcataatgaa
     5821 ggtgttcaac tccgtcatat tggtcgtctt gaaaggcttg accctgccat tcaaaagaaa
     5881 gtcttgcatg cgattgattt aaccaagaat aacgaccgct tggtgctcaa tgtggcattc
     5941 aactatggcg gacgtgacga aattgtcaat gccattcaaa acattatcaa agatggcatc
     6001 cctgctgaag aagtgaccga tgaaatggtc aatcgttact tatttacagc gggcgtgccc
     6061 gacccggatt tgattatccg tacatcgggc gaactgcgcg tcagtaactt ccttatctgg
     6121 caggcagcat actcagagtg gtacatcaca ccaacgttct ggccagactt tgacaaggaa
     6181 gaatatcacc gtgcgttggc aacttatgcc aatcgcgaca gacgatacgg aaaggtatct
     6241 tcgggggaat tacaggaatc caatgcgtag acgtctcatt accgctttgg gattagccgt
     6301 agtaggagtg cccgctattt tatttggcgg gttcctttat tacttattga tggggacctt
     6361 tttgatcggc gccgcgtggg aatatgtaca tctattcagg gctgtgaaat ttgaccccaa
     6421 tatgatcgta accgttggtg gcgtggcggc tgtcacactc actcgcatgt tccttccaca
     6481 atttgcacag cccaccctag ccattgtcgt gttattggca atggctgttc acttgattgc
     6541 ttatgagcgc ggacgagacc agtccgcgct tgattttgga gtgaccattg gcggtattgt
     6601 ctacattggt tggatcggct cctatttatt agacctgcgt aatttaccag agggtggttg
     6661 gtggtttatg ttggtcatgt tctgtgtatg ggcaggcgac tctggtgctt actccattgg
     6721 gcgtgcatat ggaaaccaca aaatggcgcc gcgccttagc ccgaaaaaaa gttgggaagg
     6781 ctatgcagcc agcgtcttta caggagcaca cactggtgca ctctatgtgt ggatcttcca
     6841 aacttttgga catcatattt atagtattac gatctggcag ggagcgctta tggggctttt
     6901 actcggcgct ttgccaacgc ttggtgacct tggcgaaagc atgatcaaac gccaatcggg
     6961 catcaaagac tcaagcgaca ttatccccgg gcatggcggt ttctttgacc gcatcgactc
     7021 atggctatgg ggttccgtca tcggatttta ttttctcacc tggttcatac tctaatctct
     7081 ggaggatttc atggaagttc tcggtgcaac acctactcga aacctcgcgc tcgagcttgc
     7141 acgtgtgacc gaagccgcgg cgatgatggc agg
//
LOCUS       DAOQFT010000043         7148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5111_length_7148_cov_5.363880, whole genome shotgun sequence.
ACCESSION   DAOQFT010000043 DAOQFT010000000
VERSION     DAOQFT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5111_length_7148_cov_5.363880"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="PLR93_01975"
     CDS             complement(<1..1267)
                     /locus_tag="PLR93_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH25753.1"
                     /translation="MQITTSQTPAVPSRPASGLLSFWRSFSRSRTGLLGFWMLVIAIF
                     VAVFAPIIAPYERGASTSVQIADIYQPPSAAHWFGTDDAGQDVLTNFIYGARVSLTVG
                     FFAAFISIVIGGLFGLVAGFFGGRWENFLMRFTDIMLVIPDLPLTVVIVALTKPSLWN
                     IIFVIGILGWTTTARVVRSQTLAVKSRKFVLRARAIGASKIHILVQHILPLVLPILVV
                     QAVLAISLAILNESTLSFIGLGDPSAPSWGQMLNFAFGRGAMSVGAWWALFAPGFGIV
                     WVVLSLTLLGQGLEQVLNPRLDTHHLMPGKPVVQNEAGAKPIQQDALLEVQNLSINYV
                     TEGKVPARAVENVSFTLKAGELIGLVGESGCGKTTLMLALLKLLPSAGQIVNGKVFYS
                     GQDITAMNEDEINTVRWSGVSIVFQGAMNA"
     gene            complement(1272..2300)
                     /locus_tag="PLR93_01980"
     CDS             complement(1272..2300)
                     /locus_tag="PLR93_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25754.1"
                     /translation="MDRTRAILRKAAWSVFTIFFVIILNFFLFRILPGDPARAGIRDP
                     RLNKDAVEALRVRFGLDKPIINCFESLNPIKIGSCSVNPLDTQFFIYVKNLLQGEMGF
                     SYHTNRPVVEVLGERLWNTILLIGVGQILAILFGVAFGIFAAWRARTSIDYASVGASL
                     VAWSLPTFWLAIVLLFWGSMIGLPVAGKATPGMSSYPLLQQWGDIAKHMLLPSITYTI
                     VYMGEYTLIMRSSLLDVLSEDYILTAKAKGLSTVQILRDHALKNAMLPLVTVIAINLG
                     FTVAGVIQIESVFSWPGLGSAIFEAVVRRDFPMLQGAFLLIAVAVIIANLLADLTYNY
                     LDPRVRVE"
     gene            complement(2373..4007)
                     /locus_tag="PLR93_01985"
     CDS             complement(2373..4007)
                     /locus_tag="PLR93_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH25755.1"
                     /translation="MKQKVLYLLTLLALVLSACTGGAANQPAIARVGWAGSPDTLNPG
                     MAILTEAYTMFELVYDSMYDLNLDGSFKLSLAESATVSDDGIVWTFKIRDGIKWHDGQ
                     PLTAEDIAWTYNLYKDTPEYPYLNGYYTTYFDTIEATDNNEVVLTLTEAIPNIESQLV
                     FLYILPKHIWENVDKLEYTNTEMIGSGPFKMTEYVQNEFVHLTANKDYFGGAPKIDEV
                     IFQTFENQDALVQAIKTGQVDMITEMPNTAAESLKSEKDIELVVGAPLAPGVTDIIFN
                     QVDPENCPAEGGLCTGHPALRDRNVRLALAHATDKQNLIDVILLGLGKPGLTLIPDGL
                     GVWYNDSITDYEFDVAKANQILDDAGYIDADGDGIRDMPDGSRPLTFRLNWPSDSIPA
                     PRMAELLAEMWGKVGISLEPQAVDPDALTAQCCPAFDFDIMIWGWASDPDPSSLLYVY
                     TSEGIPTGSSETGYSNPEYDALYAKQQVELNFDARKQLVWDMQKIVHDDVVYIIPFYD
                     ANVQAYRTDRFTGWITDQAKVELSDVTSLTVIEPVK"
     gene            complement(4165..5148)
                     /locus_tag="PLR93_01990"
     CDS             complement(4165..5148)
                     /locus_tag="PLR93_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25756.1"
                     /translation="MSEPLVRVENLTKHFPIRKGMLIERVVGAVRAVDNISFTIARGE
                     TLGMVGESGCGKTTAGRTLLGLYPATSGKTIIDGHSVHEARGSELMKVRRKAQMIFQD
                     PYASLNPRWTVSSIISEPLRVHGLLSSQKARDERVKELLELVGLNARHINRFPHEFSG
                     GQRQRIGIARALASDPLFIVCDEPISALDVSIQAQVVNLLEKLQDQLGLTYLFVAHDL
                     SMVRHICDRVSVMYLGVMVELAERDELYENPQHPYTKALLSAVPVPDPRKERERKRVI
                     LSGDVPSPINPPSGCRFHPRCPIAVDRCKAEVPEWREIKPGHHVACHLVEK"
     gene            complement(5145..6140)
                     /locus_tag="PLR93_01995"
     CDS             complement(5145..6140)
                     /locus_tag="PLR93_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25757.1"
                     /translation="MKTDHKFLEVKDLKTQFNTPEGTVYAVNGVSFSLNEGETLAVVG
                     ESGCGKSVTMMSIMKLIQMPPGKIVGGEVIFRDSDLLKLSEEAMEQIRGREIAMIFQD
                     PMTSLNPVLTVGRQITETLRTHTPMTPEQCRDRAAQLLELVGIPDPVKRLDDYPHQFS
                     GGMRQRVMIAMALACTPSLLIADEPTTALDVTIQAQIVELVKDLKKSVGMAMIWITHD
                     LGVVAGMADRVLVMYAGYVVEEAPVDDLYENTLHPYTLALLQALPRADRRRDQSLKSI
                     KGSPPNLLMEPKGCPFAPRCEFAQEKCIHENPTLAEFEPNHKVACWVDVKTGALR"
     gene            complement(6147..7097)
                     /locus_tag="PLR93_02000"
     CDS             complement(6147..7097)
                     /locus_tag="PLR93_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25758.1"
                     /translation="MSTVQASNQSGTMIAEIEAPSLSLRDLTWRRFRRHKMAIVGLGV
                     LILLIIYSFGGAFFVTEKYSNFTNTKERLQPPSAEHPFGTDTVGRDLMARTIYGGQIS
                     IIIGITAAFVEVMMGILIGAIAGYFGGKIDAFLMRFTEAMLNLPQLLLLIVMAKFFSS
                     DIKNINLFGREFSGSVVVIIFIIGLTSWPYLARIVRAEFLSIKEREFILAARATGTPT
                     WEIIFKHILPNSIAPIVVSATLGVAGAISLEAYVSFLGLGVRAPTATWGNMLENAYRY
                     IESAYWMWLFPGLFIVLIVLSINFLGDGLRDALDPRSRTV"
BASE COUNT         1982 a         1806 c         1679 g         1681 t
ORIGIN      
        1 gcgcattcat agcaccttga aacacaatcg aaacaccgct ccaacgaact gtattgattt
       61 catcttcgtt catggcagtg atatcttgcc cgctataaaa aactttgccg ttcacaattt
      121 gtccggcaga cggtaatagt tttaacaacg ccaacatcag tgttgtcttg ccgcaaccgc
      181 tctcccccac caaaccgatc aactcacccg ctttcaacgt gaagctaaca ttctccactg
      241 cacgcgcagg cactttccct tcagtgacat agttgataga aagattttgg acttccagca
      301 aagcatcttg ctggataggc ttcgcccccg cctcattctg gacgacaggc ttgcccggca
      361 ttaagtgatg tgtatccagc cgtgggttga gtacctgttc caagccctgc ccaagcaaag
      421 tgagcgagag cacaacccac acaatgccaa agcccggagc aaacaacgcc caccatgcac
      481 cgacagacat cgctccgcgc ccgaaggcga agttgagcat ttgcccccaa gaaggtgcag
      541 aagggtcgcc caaaccgatg aaggacaacg tgctttcgtt caagatggca agcgaaattg
      601 caagaactgc ctgaaccacc aaaatcggca gaaccaacgg cagaatatgt tgaaccagaa
      661 tatggatttt acttgcgcca atggcacgag cacgtaaaac aaatttacgc gatttcaccg
      721 ccaaggtctg cgagcggact acacgtgcgg tcgtcgtcca gccaagaatg ccgatgacaa
      781 aaataatgtt ccagagagaa ggcttggtca acgctacgat cacaaccgtc aatggcaggt
      841 ctgggatgac gagcatgatg tctgtaaagc gcatcaggaa gttttcccaa cgcccgccga
      901 agaagccagc gaccaagccg aataagccgc caatgacaat ggaaatgaaa gcggcaaaga
      961 aacctacggt caatgagacg cgcgcgccgt agataaagtt cgtgagcaca tcttgcccgg
     1021 catcgtctgt gccgaaccag tgagctgcac tcggcggctg ataaatatcc gcgatttgta
     1081 cagaggtgga tgcgccacgt tcatatggcg caatgatagg agcaaatact gccacaaaga
     1141 ttgcaatgac cagcatccaa aaaccgagta gacccgtacg gctgcggctg aaggaacgcc
     1201 aaaaggagag caacccagat gcaggtcgag aaggaaccgc aggagtttga gaagttgtaa
     1261 tctgcattga gttactccac gcgcactcga gggtcaagat agttataggt taggtctgct
     1321 agcaagttgg cgataataac ggcaacagca atcaatagaa aggcgccttg cagcatcgga
     1381 aagtcgcggc gaactacggc ttcaaaaata gcactcccca agccgggcca agagaaaacc
     1441 gattcgatct gaataacgcc agccaccgta aagccaaggt taatcgcaat gacggtgacg
     1501 agcggaagca tggcgttctt gagggcatgg tctcgcaaaa tttgaaccgt gctcaatcct
     1561 ttggctttgg cggtcagaat gtaatcttca gaaagcacat ctaacaagct agaccgcata
     1621 atcagggtgt attcgcccat atatacgatc gtgtatgtaa tagaaggcag aagcatatgc
     1681 tttgcaatat cgccccattg ttgcaatagc ggatacgaac tcataccggg cgtggcttta
     1741 cccgcaacag gtaagccgat catactgccc cagaagagca aaacgatagc aagccaaaag
     1801 gtcggcaaac tccacgcgac caaacttgcg ccgacggaag cataatctat ggaagtgcgc
     1861 gcacgccaag cagcaaaaat accgaatgcc accccaaaaa ggatggcaag gatttgtcct
     1921 actccaatta acaaaatcgt gttccacaat cgttcgccaa ggacttctac aacgggacgg
     1981 ttcgtgtgat agctaaaccc catttcacct tgtaaaagat ttttcacata aataaagaat
     2041 tgtgtatcca gcggattaac cgagcaactc ccgatcttga tcgggttgag cgactcaaag
     2101 caattgataa ttggtttatc taacccaaag cgaacgcgca acgcttccac cgcatctttg
     2161 ttcaaacgcg ggtcgcggat tcccgctcga gcggggtcac cgggcaggat acgaaataga
     2221 aaaaaattca aaataatgac aaagaatatt gtgaaaacag accaagctgc ttttcggaga
     2281 atggcgcgag tgcgatccaa gggttttgtc tcttgaacgt tgcccaacag gttcaatcaa
     2341 cctgttgggc aacgtctttc atttcaaagt ttttacttca caggctcaat aacggtcaaa
     2401 gatgtgacat ctgagagttc cactttggct tggtcggtaa tccatccagt gaaacggtct
     2461 gtgcggtaag cttgaacatt ggcgtcgtag aatggaatga tgtacacaac gtcgtcatgg
     2521 acaatctttt gcatatccca tacaagctgt ttgcgggcgt caaagttcaa ctccacttgt
     2581 tgtttggcgt agagggcatc atattcaggg ttggagtagc ctgtttcgct ggaacctgtc
     2641 ggaatgcctt cagacgtgta aacatataac aaagagcttg ggtcagggtc agatgcccaa
     2701 ccccagatca taatgtcaaa atcgaatgca gggcagcact gggcagtcaa agcatcggga
     2761 tccacggctt gcggttcgag gctaatacca actttgcccc acatttcggc gagaagctcc
     2821 gccatgcgtg gagcggggat actgtcgctg ggccagttca agcggaaggt caaggggcgt
     2881 gagccgtctg gcatgtcacg aatcccatct ccatcggcat cgatgtaacc agcgtcatca
     2941 aggatctggt ttgctttggc aacatcgaat tcatagtctg taatagagtc gttgtaccaa
     3001 acgcccaaac catcaggaat caatgtgaga ccgggtttac caagccccaa caagatgacg
     3061 tcaatgagat tctgcttgtc tgtggcgtgt gcgagcgcaa ggcggacgtt gcggtcacgc
     3121 aaggcagggt gacccgtgca aagcccacct tctgcaggac agttttcagg gtcaacttga
     3181 ttgaaaataa tatcggttac acccggggca agcggcgcgc cgacaaccaa ttcgatatct
     3241 ttttcagatt tcaacgattc ggcagcagta ttcggcattt cagtgatcat gtccacttga
     3301 ccggttttga tcgcctgaac caacgcatct tgattttcaa aggtctggaa gatgacctcg
     3361 tcaattttcg gagcgccacc gaaatagtct ttgttggctg ttagatgcac aaattcattt
     3421 tgtacatact ccgtcatctt gaacggaccc gagccgatca tttctgtatt ggtgtattcc
     3481 agtttatcga cattctccca aatatgcttc ggaaggatat acaggaatac caactggctc
     3541 tcaatgttcg gaatggcttc agtcagggtc agaacgactt cattgttatc ggtcgcttcg
     3601 atggtgtcaa agtaggtggt gtaatacccg ttcaagtagg gatattctgg agtgtctttg
     3661 tagagattat aggtccaggc aatatcttct gctgtcaaag gttgaccatc gtgccatttg
     3721 attccatcgc ggattttgaa cgtccatacg ataccatcat ctgatacggt ggcgctctcc
     3781 gccaagctaa gtttgaacga gccgtctaaa ttaaggtcat acattgaatc gtagaccaac
     3841 tcgaacatgg tgtacgcctc ggtcaagatt gccatgccgg ggttgagcgt atctgggctg
     3901 ccagcccagc ctacacgggc aatggcgggt tgattcgctg caccgccagt acaagccgaa
     3961 agaaccaagg caagtaaggt gagcaaatac aatactttct gtttcataac ttctcctccg
     4021 atgactgatt agcttctata aaataaaatg cgccattgca gaaaacacgg caaggcgcat
     4081 taattaactg gtttctcagg gatgaggttg ggctccttca taacctgagc taaattttac
     4141 tgtagtttac agattaccga aagttcattt ctctactaaa tgacaggcaa catgatgccc
     4201 aggcttgatt tctcgccatt cgggcacctc tgctttacag cgatccacgg caatggggca
     4261 acgcgggtga aagcgacaac cagaaggcgg attgattggg ctgggcacat ctccgcttag
     4321 gatgacgcgc tttcgctccc tttctttgcg cgggtctggc acaggaacgg cggaaagcaa
     4381 agcctttgta taggggtgtt gtggattttc atatagttca tctcgttcag ccaattccac
     4441 catcacaccc aaatacatca ccgagacacg gtcacagata tggcgaacca tgctgaggtc
     4501 atgagctaca aagaggtatg tcagtccaag ttgatcttga agtttctcca acaagttaac
     4561 gacttgtgcc tgaatagata catccaaagc cgaaatcggc tcatcgcaga cgatgaacag
     4621 cgggtctgaa gccagggctc gagcgattcc aatgcgctga cgttgtccgc cagagaactc
     4681 gtgcgggaag cgattgatat gccgagcgtt caaaccgacc aattccaaaa gctcttttac
     4741 tcgttcatct ctcgcttttt ggcttgaaag caagccatgt actcgcaaag gctcactgat
     4801 gatcgaactt accgtccaac gagggttcag ggatgcgtag gggtcttgaa agatcatctg
     4861 cgctttgcgg cggactttca tcagttcact accacgggct tcatgcacac tatgcccatc
     4921 aataatggtt ttgccagaag tagcagggta taacccaagc aaagtacgcc cggcggttgt
     4981 cttaccgcag ccactttcgc caaccatgcc caaggtttca ccgcgcgcaa tggtgaatga
     5041 aatattgtcc acagctcgta cagcgccaac cacacgttcg atcaacattc cttttcgaat
     5101 tgggaaatgt ttggtcaggt tttcaactcg taccaatggt tcgctcatcg caatgctcca
     5161 gtctttacat ctacccagca ggcgacttta tggtttggtt caaattcagc taatgtaggg
     5221 ttttcatgga tgcatttttc ttgagcgaat tcacagcgtg gcgcgaaggg acatccttta
     5281 ggttccatca acagatttgg cggagagccc ttaatagatt tcaagctttg gtcgcgtcgg
     5341 cgatctgctc gtggtaaagc ttgcagtaac gccaaggtat agggatgcaa tgtattttca
     5401 tacaggtcat ccacaggagc ttcttccact acatacccgg catacatcac aagcacacga
     5461 tctgccatac ctgccacaac acctaaatca tgcgtgatcc aaatcatggc catgcccacg
     5521 cttttcttta ggtctttcac caattcaaca atctgcgcct gaatcgtcac atccaatgcc
     5581 gtggtcggtt catctgcgat caacaaactg ggcgtgcaag ccaacgccat ggcaatcatc
     5641 acacgctggc gcatgccgcc agaaaactga tgtggataat catccaaccg tttgactggg
     5701 tctggaatcc caacaagttc taataactga gcagctcgat ctcgacactg ttcaggcgtc
     5761 ataggagtat gagtgcgcag cgtttctgtg atttgacgac ccacggtcaa cacaggattt
     5821 agactggtca ttgggtcttg gaagatcatc gcaatctcac gcccgcggat ttgttccatc
     5881 gcctcttcac ttaatttgag caggtcgctg tctcgaaaga tcacttcccc accgacaatt
     5941 ttcccgggtg gcatctgaat taatttcatg atggacatca tcgtcaccga tttgccacaa
     6001 ccactttcgc ctacaacagc tagtgtctca ccttcgttca agctaaacga aacgccattc
     6061 acagcataga cagtcccttc cggtgtattg aactgcgtct tgaggtcttt tacttcaagg
     6121 aatttatggt ctgttttcat aagtcattac accgttcggc tacgaggatc aagagcatca
     6181 cgcaagccat cacccaaaaa gttgatgcta agcacgatca aaacaataaa taaaccgggg
     6241 aacagccaca tccaatacgc agactcaatg tagcgatagg cgttttcgag catattaccc
     6301 caagtcgctg ttggtgcacg aacgcctaag cctaaaaagc tgacatatgc ttcaagggag
     6361 attgcgcccg caacgcccaa ggtagcggat acgacgattg gagcaatgct attcggcaga
     6421 atatgcttga aaataatctc ccaagtaggg gttccagttg cgcgtgccgc aagaataaac
     6481 tcacgttctt tgatagacag aaattcagca cggacaatac gcgccaaata tggccaactc
     6541 gtaagtccaa tgatgaagat aattacgacc acactgccgc taaactccct tccaaagaga
     6601 ttaatatttt taatatcact ggaaaagaac tttgccatta cgatcaacaa aagcaattgc
     6661 ggcaaattca gcatggcttc ggtaaaacgc atcagaaagg catcgatctt cccaccaaaa
     6721 tagccagcaa tcgccccaat gagaattccc atcatcactt ctacaaaagc ggctgtgatc
     6781 ccgataatga tagagatctg cccgccataa attgtacgag ccataaggtc tcgtccaacg
     6841 gtgtctgttc cgaagggatg ttccgcgctc ggcggttgta gtcgttcttt ggtattggta
     6901 aagtttgaat acttctccgt aacgaaaaac gctccgccaa aagaataaat aatcaaaaga
     6961 ataagaaccc ccagcccgac gatcgccatt ttatgacggc ggaaacgtcg ccaagtaagg
     7021 tctcgcaggg ataatgaagg tgcttcgatc tcggcgatca tcgttccaga ctgattggaa
     7081 gcttgaaccg tggacataac tctctcctaa tcaaagcgaa tacgcgggtc aagccaggcg
     7141 tatacaat
//
LOCUS       DAOQFT010000044         7137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5119_length_7137_cov_6.665349, whole genome shotgun sequence.
ACCESSION   DAOQFT010000044 DAOQFT010000000
VERSION     DAOQFT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5119_length_7137_cov_6.665349"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="PLR93_02005"
     CDS             complement(<1..1883)
                     /locus_tag="PLR93_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25759.1"
                     /translation="MNNKRSLGCVLTGALIILVICGAIAGFATWRLNTRGPEILVEIK
                     RPQTTMLANVNEALPLIVSAEASDVITRLEVYADGALIAATNGEGQNAAMLAQTWTPS
                     TVGRHVLVARAFLDAEHFSDSEVVFVDALDLSALPLQVNVDDLPRGEGVTEISVGDLA
                     TASGTTLEEIVRLNPSLSSTGSIPSGTLVSIPRLAPEPGIGSPVPPPAPGAPGSPAPS
                     TSDPHFEGETHSCNQIAMRWTGSDFETGYTLYRLAPGDSSMTLVATLPVTTVSYADNS
                     ISRAGTYRYFLSPIWRGGGSGITSMLAVEIPADCAPVSSTSANLRLSLMSLTAQESFT
                     GVYCYVSVNGSRYERLPAGDGLLRPTSGNLYYDLPLQLPNRGQYTLGPTADGLVRLEG
                     ECWGRRGVESVRIGRFAGNHASSEWDGRDLTSDVAFEPIEVASLDTIPLMTGGSNFIR
                     YRIQSAASHFDLTGLYNGAIQVPLAITDPFRIENPTVPPPTNVRLVNRASGICEDLPM
                     DDPNLGTILCQEQIVRHIAWDWSASEQVPQSAVTGFMLITSIEDAISTTPAGEGYTHN
                     VNSGTARSAPVPNYSQRWRCGATVRFTVRAVTNFGMSEPSEAFEVRLPACPSARNLLI
                     RIES"
     gene            complement(1873..2106)
                     /locus_tag="PLR93_02010"
     CDS             complement(1873..2106)
                     /locus_tag="PLR93_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25760.1"
                     /translation="MNERRKAASFLIRVWQEPSQWQPPGEWRVSAHPLEGGGEVLFNS
                     ATELWRYLTHADSQIQSTPQTKKIISEGEENEQ"
     gene            complement(2119..2736)
                     /locus_tag="PLR93_02015"
     CDS             complement(2119..2736)
                     /locus_tag="PLR93_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25761.1"
                     /translation="MRNLLLAIFILALASMACNMPGSAGSPVNPPIEIPTFTATVPSV
                     VSDKPTDLPAPATSTPEQAVLQPQVNTFDPRSIADCDIFVDSDFPNTIGALPDTKKAL
                     PETDKKNCQYNFANGTLFVSIFTGMPGMEAFENVRQFDALTGATIIPYPIGEAAVFKD
                     FGDGHITLEAVINGWYVVLDARGFDEKNLALLAELLLANLAPYSP"
     gene            complement(2839..6120)
                     /locus_tag="PLR93_02020"
     CDS             complement(2839..6120)
                     /locus_tag="PLR93_02020"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH25762.1"
                     /translation="MAFSLWADEDEASSLANLRRHLYLLKKSLPKALQPSLIISTQTV
                     LFQFPANTWVDVLAFEQEPISLQEMEEHALFYTTDLAQGAETDEFIFSRREELRNRYA
                     QLLRMLAQTYFNYAEYETSMYWVRKLIAQDPWDEEALRLYMTSDFILGNRTTALSTYK
                     SFEKKLRLEMDIQPMPETMALYRDILHNHLLRPVSSTKTEGETFISRLSEIQTLGNAI
                     QAHGQYGNFVIINGTAGVGKTTLVREALRRFLNSSKAKLLWGDCQPAGEEQPYSIWQQ
                     IFNAAVPLLARQQDVAQEWLNHLLPLIPDLGLLRADLFSPTRPDATELRTAMRQTLFA
                     LSESRPLIIVIEDAHWMDVPSLEFLRELAESSLNIPVLFILTHRTDDAALPLFEIKRI
                     LRRKRNVVDISLQPFTTTETNQYLEKFLGTKTLPKELIDDLMQYSGGLPLLLHEVTQT
                     LNSSKRILSLREAFLFRLDRLSPAAREMVETAAVIGFSLTDIELRTALGWAAELYAST
                     MDALQAERFLLENPSNSADEYAFSHRLIHEIIVEQISVERISNLHIRVARALEQIHSE
                     DIGFAAEIALHYQQAGQLILAAGFWVRHASESTDLAAFDSGLAAINQAEAILTGASLD
                     AQEVHAKLALQRGVIVFYQGKSEEAFSLLEKAVEHSKLFPPLYAHALSMQAYAFHTHD
                     RNELAFQAASQAFDLSMATKDVSNAVRALNIRAVSALMLGKTRGALDDLQQALALLEQ
                     HNLSASTQTVQSLYHLGTALVFSQDYQQARAILSRAVELSKKGGLRRLEAAALTMLGQ
                     IALNCGQYKLAVEIYDRSIEVAGESYLPGMWGKFAGRGWANLRMGNLLAARTDFERGL
                     DVSIQVESQYGTILMRSYLAFTALAAGESPSASFADLEADALTQALHPVVLFNANIQS
                     QGWRLLRDSEKANSAHHRAIEAAKNSDVPSFVQSARLQKLYTQVILEENIDPTYMEEE
                     LAELAKLTLDSGEMPLQVNTFLVRAWLHSRMGNLQEALSTATQALTLARACPDLPWIG
                     ECQVLLAQLFTKLGDQNQAKICTEEVHALAKTSFAPLTVKFGGEPLALVQEHLLKSLT
                     PTHLN"
     gene            6386..>7137
                     /locus_tag="PLR93_02025"
     CDS             6386..>7137
                     /locus_tag="PLR93_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNH25763.1"
                     /translation="MSQSPTIKIYLLGSVHLERGKETLEFARQKTLLLLVYLALHPQE
                     HPRERIASIFWKDATTDEAARLSLRVTINDLRKTLGEDSLIGGRDSLQLNPDLNLWID
                     VREFQRLTDSRATPEDWQSALSLYTGDLLPQVYEDWVLEWRAEFEQACLETRLRLAQH
                     QRGQGQYVAAIETARLALEIEPVRESTHQLIILCYESLGDREAALLQYESCSKALREI
                     GVEPSPELQGIYRRLQKTPASSHIAGNLPRPLT"
BASE COUNT         1924 a         1696 c         1712 g         1805 t
ORIGIN      
        1 gactcgatcc ggatcaaaag attgcgagct gatgggcaag ccggcagccg cacctcaaag
       61 gcttcgctcg gttctgacat gccgaaattt gtaacagcac ggacggtgaa tcgcacggtc
      121 gcgccgcaac gccaacgttg tgaatagttt ggaacgggtg cagaacgcgc agtccccgag
      181 ttgacattat gggtgtagcc ctcaccagcc ggggtagttg agattgcatc ctcgatagaa
      241 gtgatgagca tgaaacccgt cactgctgat tgcggcactt gttcgctagc gctccaatcc
      301 catgcaatgt gacggacgat ctgctcctga cacaagatcg taccaagatt ggggtcgtcc
      361 attggcaggt cttcacaaat gccagatgca cggttgacca aacgtacgtt cgtgggcggc
      421 ggaacggtag ggttttctat tcgaaatgga tctgtaatcg caagcggaac ctgaattgcg
      481 ccattgtata aacccgttaa gtcaaaatgg ctggctgcag attgaatgcg atagcggata
      541 aagttggaac cacccgtcat aagaggtatg gtgtctagcg aagcaacttc aatgggttca
      601 aatgccacat cactagttaa gtcgcgtcca tcccattcac tactggcgtg attgcctgca
      661 aacctcccaa tgcgcacact ttctactccg cgcctgcccc aacattctcc ctctaaacga
      721 accaagccgt cggcagtggg accaagggta tattgtccgc ggttgggcaa ttgcagagga
      781 aggtcgtaat ataaattacc agaggtgggg cgcaacaagc catcccctgc tggaaggcgt
      841 tcataacgtg acccattgac cgagacataa caatacacgc cggtaaaact ttcctgcgca
      901 gtgagactca taagtgaaag acgcaagttt gctgatgtgc tggaaactgg tgcacaatcg
      961 gcaggaattt ccactgccag catggatgtg ataccactgc caccgccacg ccaaatggga
     1021 gaaaggaaat agcgatatgt tccggctcgg gaaatcgagt tatctgcata agaaacggtt
     1081 gttactggca aagtcgcaac aagcgtcatg gagctatcgc ctggggcaag tcgatagagg
     1141 gtatagccag tttcaaagtc actgccagtc cagcgcatcg cgatctgatt acaggaatgc
     1201 gtctcacctt caaagtgcgg gtcgctggtt gaaggtgcag gggatcccgg agcacctggg
     1261 gcaggcggcg gcacagggct accaatacca ggttcgggag caaggcgcgg aatagaaaca
     1321 agcgtacctg aaggaatact tccagtagag gaaagacttg ggttcaaccg aacaatttct
     1381 tccagcgtgg ttcccgaggc ggtggctaag tcacccactg aaatttcagt cacaccttca
     1441 ccgcgcggca ggtcatctac attcacttga agtggcaatg cactcaagtc aagcgcatcc
     1501 acaaaaacaa cttctgaatc cgaaaaatgt tcggcatcta aaaatgctcg cgctacaagc
     1561 acatgtctcc cgactgtaga gggcgtccag gtctgtgcca acatggcagc attttgcccc
     1621 tcaccattgg tggcagcaat caaagcacca tctgcataca cttcaaggcg ggtgataaca
     1681 tcgcttgctt cagcagagac aatcaacggc aacgcttcat tgacattagc aagcatggtc
     1741 gtttgtggtc ttttgatctc aaccaagatt tcaggtccgc gtgtattcaa tcgccacgta
     1801 gcaaagcctg ctatggcgcc gcaaattaca agaattatca acgctcccgt tagaacacaa
     1861 ccaagactgc gtttattgtt cattctcttc tccttctgaa ataatcttct ttgtttgagg
     1921 ggtactctgg atttgcgagt ctgcatgggt gaggtagcgc catagctcag ttgccgagtt
     1981 gaacaagacc tcaccgcctc cttccaacgg gtgagcgctt actcgccact cgccgggggg
     2041 ctgccactga ctcggttcct gccaaacccg aatcagaaaa gatgctgctt ttcgtctttc
     2101 attcatggat aagctccgtt aaggcgaata gggagccaga ttggcaagca ataattccgc
     2161 cagcagtgcc agattttttt cgtcgaagcc gcgtgcatcc aataccacat accaaccgtt
     2221 gatgactgct tcgagtgtga tatgtccatc accaaaatct ttaaatacgg cggcttcacc
     2281 gattgggtat gggattattg ttgcacctgt cagagcgtca aactgacgga cattctcaaa
     2341 ggcttccatc cccggcatgc cggtgaatat ggaaacaaag agtgtgccat ttgcaaaatt
     2401 gtattggcag ttttttttat cagtttcggg aagagctttc ttcgtatctg gaagagcgcc
     2461 aatggtgttt ggaaaatcgg agtcaacaaa aatatcgcag tcagctatac tgcgcgggtc
     2521 aaatgtgttg acttgcggtt gcaatacagc ttgctctgga gtggatgttg caggggcggg
     2581 caagtcagtt ggtttatctg acactacact gggaaccgta gctgtaaaag taggaatctc
     2641 aatgggcggg ttgacaggcg agccggctga accaggcata ttacaagcca tcgaagccag
     2701 agccaaaata aaaatagcca atagcaggtt tcgcatgctt ttctctcctt tttgccccat
     2761 atcttacaga gagagcgtcc taaaaacgtc ctctaaacaa gtgtttcccc ttaccttcaa
     2821 gagtttagta cggaaggact agttcaaatg tgtgggtgta agacttttca ataaatgctc
     2881 ttgcacaagc gctaaaggct ctcctccaaa tttaacggtt aaaggtgcaa aggaagtttt
     2941 cgccaaggca tgcacttctt ctgtacatat ctttgcctga ttttgatccc caagttttgt
     3001 gaaaagctgg gcaagtaaga cttggcactc tcctatccat ggcaggtctg ggcaggcgcg
     3061 cgccaaggtt agtgcttggg tcgcagtgga aagcgcttct tgtaaattgc ccatacgaga
     3121 atgcaaccac gcccgcacca aaaatgtgtt gacttgtaat ggcatttcac cagaatcaag
     3181 agtcagctta gctaattcag cgagttcctc ttccatgtaa gttgggtcaa tgttctcctc
     3241 aaggatgact tgcgtatata gtttttgcaa tcttgctgat tgaacaaaag aaggaacgtc
     3301 actatttttg gcagcctcga tcgcgcggtg gtgtgccgaa tttgcttttt cgctatccct
     3361 aagtaagcgc cacccctgag attggatatt ggcattaaaa agcacaacgg gatgtaaggc
     3421 ttgggtcagc gcatccgctt cgaggtccgc aaatgaagcg gaaggagatt ctccagctgc
     3481 aagtgcagtg aacgccaagt aagaccgcat caatattgtt ccatattggc tttctacttg
     3541 aatagaaaca tctaacccgc gttcaaagtc tgtgcgagct gccagtagat ttcccatccg
     3601 taaatttgcc caaccccgcc cggcaaattt accccacata ccaggcaggt acgactctcc
     3661 agcaacttca atggatcgat cgtaaatttc aacagcaagt ttgtattggc cacagttcaa
     3721 agcgatctga cccaacatag tcaacgcagc cgcttctaga cgacgcagtc cgcccttttt
     3781 tgaaagttcc actgcacggg aaagtatggc tcgtgcttgt tgataatcct gtgaaaagac
     3841 aagagcagtg ccgagatgat acaaactttg tacagtttgc gttgatgcag aaaggttgtg
     3901 ttgttccagt aaggcaagtg cttgctgaag atcatccaac gcgccacgtg tctttcccag
     3961 catcaaggca ctcactgcgc gaatgtttaa agcacgcacc gcattcgaaa catcttttgt
     4021 tgccatggaa aggtcaaatg cttgcgaagc agcttgaaat gcaagttcat tgcggtcgtg
     4081 ggtgtgaaaa gcataggctt gcatcgaaag agcatgtgca tatagtggcg ggaatagttt
     4141 tgaatgttcc actgcttttt caagtaaaga aaaagcctct tcgctttttc cttggtaaaa
     4201 cacgatcacc ccgcgttgaa gagcaagttt agcgtgcacc tcttgagcat ctaaacttgc
     4261 accagtaaga atggcttcgg cttggttgat ggcagcgaga ccagaatcaa atgcagccag
     4321 gtctgtactt tcgctagcat ggcgcaccca gaagccggca gccaagataa gctgaccggc
     4381 ttgctgataa tgaagggcaa tctctgccgc gaatccaata tcttctgaat ggatttgttc
     4441 caatgcgcgt gccacacgaa tatgtaaatt tgaaattcgc tctacagaga tctgctctac
     4501 aataatttca tgaatcaggc ggtgggaaaa agcatattcg tctgcactat ttgaaggatt
     4561 ttccaaaaga aaccgttctg cttgcaaagc atccattgtg gaggcgtaaa gttccgctgc
     4621 ccaacccaga gccgtacgca attcaatgtc ggtcagggaa aatccaatca cagcagccgt
     4681 ctctaccatt tcacgtgcag ccggtgagag acgatctaac cgaaagagaa acgcctcgcg
     4741 caaagaaagg attctcttgc ttgagttgag ggtttgggtc acctcatgca ataataaagg
     4801 cagtccacct gagtattgca tgaggtcatc tataagttct tttggaagag tctttgttcc
     4861 caaaaatttt tcaagatatt gatttgtctc cgttgttgta aatggttgca gagagatatc
     4921 tactacattt cgcttacggc gtaatatccg tttgatttca aacaaaggca atgcagcatc
     4981 atctgtgcga tgggtaagaa taaaaagaac aggaatgttt agagagcttt cagcaagctc
     5041 tcttaagaac tctaaggatg ggacatccat ccaatgtgca tcttcaatca ctatgataag
     5101 cggacggctc tctgacaagg caaataaagt ttgcctcata gcggtgcgca attcggtagc
     5161 atctggacgg gtaggggaaa acagatcggc tcttaacaag cccaagtctg ggatgagcgg
     5221 cagtaaatga tttagccact cctgagccac atcctgttga cgcgcaagca aagggactgc
     5281 ggcattaaaa atttgctgcc aaatcgagta aggttgttct tcccccgcag gctggcaatc
     5341 tccccaaagt agtttggctt ttgatgaatt taaaaatcgc cgaagcgctt cacggaccaa
     5401 agtggtcttt cccacaccgg ctgttccatt gatgatcaca aaattgccgt attgcccatg
     5461 tgcctggatc gcatttccca atgtttgtat ttcacttaac cggctaatga atgtttcacc
     5521 ttctgttttt gtggaagaaa caggacgtaa aaggtggtta tgcaaaatat ctcggtatag
     5581 tgccatggtt tctggcatgg gctggatgtc catttccagc ctcaactttt tctcaaatga
     5641 tttataagtt gaaagagcag ttgtgcgatt accaagaatg aagtctgaag tcatatacaa
     5701 ccgcagggct tcttcatccc atgggtcttg agcaatcagt tttcgcaccc aatacataga
     5761 tgtctcgtat tcggcatagt tgaagtatgt ctgtgccagc attctcaaaa gctgtgcata
     5821 tcgattacgc aattcttcgc gacgagaaaa aatgaactca tctgtttctg ccccttgtgc
     5881 aaggtctgtt gtgtaaaaca gggcgtgttc ttccatttct tgcaaagaaa tcggttcctg
     5941 ttcaaacgca agcacatcca cccaggtgtt agcagggaat tgaaataaaa ccgtttgtgt
     6001 agagatgatc aatgatggtt gtagggcttt tggaagagat tttttcagga ggtaaagatg
     6061 ccgccttaaa ttcgcaagag aactggcttc atcctcatct gcccagagtg agaatgccaa
     6121 atgtttccgc ccatgtgggg aattcgcctg aaggacaagt cgggtgaata gacatagtaa
     6181 tttatcgcgt gcaggcaggg cagatataac gccattaact tcaatcctag gcgatccaaa
     6241 taaaaaaatg cgaatcctgt atgcctgcga catgaagaaa ttataacgat tcacattaaa
     6301 agatttggaa tggcgaccct attaaggggg catggtacac tctgtaaaaa acacatctta
     6361 ataatctttg atttcctctt ttaatatgtc acagtcacct acaatcaaaa tttacttgct
     6421 tgggtcagtc caccttgaac gcgggaagga aacacttgag ttcgcgcgtc aaaaaacttt
     6481 actcttattg gtatatcttg ccctccaccc gcaagagcat ccgcgcgaac ggattgcctc
     6541 catattttgg aaagatgcta ctaccgacga agctgccagg ctttcgcttc gtgtgacgat
     6601 caacgacttg cgtaagactc tcggcgaaga tagcctaatt ggcggtcgcg actcgttgca
     6661 acttaaccct gacttaaacc tatggattga tgtacgcgag tttcaacgcc taactgactc
     6721 acgtgctacg cccgaagatt ggcaatctgc actgagttta tatactgggg acctacttcc
     6781 acaagtttat gaagattggg tattggaatg gcgggcagaa tttgaacaag cttgccttga
     6841 aacccgttta cgccttgctc aacatcaaag aggtcagggt cagtatgtgg ctgcaattga
     6901 aaccgctcgc cttgcgctag agattgaacc cgtccgtgaa tccacgcatc aattgatcat
     6961 cctctgttat gagtcacttg gagatcggga agcagcgctc ttacaatacg aatcgtgcag
     7021 taaagctcta cgagaaattg gcgtagagcc atcgccagag ttacaaggta tttatcgccg
     7081 cctgcaaaag acacctgcat cttcccatat agcaggcaac ctcccccgcc cattgac
//
LOCUS       DAOQFT010000045         7040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5206_length_7040_cov_7.333715, whole genome shotgun sequence.
ACCESSION   DAOQFT010000045 DAOQFT010000000
VERSION     DAOQFT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5206_length_7040_cov_7.333715"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLR93_02030"
     CDS             <1..273
                     /locus_tag="PLR93_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25764.1"
                     /translation="ICLVEIVYLMEKGRISPEMKSMLDEELKSGESGLVLADLNMDVV
                     DQIANVLRDDIPDLPDRVIAATALALNLPLISRDRKIVLSGLDTIW"
     gene            408..4913
                     /locus_tag="PLR93_02035"
     CDS             408..4913
                     /locus_tag="PLR93_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH25765.1"
                     /translation="MLKTIKQFFTPPVFPDEDDKTRNAQILYALFASMMGILALLMLA
                     NIFVFVAKAIVSILLFILFIWILVARALAQSGKILLASRIFVSGLWILTTFQLLLSGQ
                     LSAAALAFHVSNLSIAGVLLKKRSAITLAVMSSLVGLGMVILEITGYSLHRYFPNPPI
                     VYWITWVIAFIMTIIPLNLTVQSAGDALARAQKSERRYVTLFNKAPMMYLIIRNEGDI
                     PIISDCNFTFLKNLGYTREQVIGQPLANFYTPQSRLMLLKEGGFQSSFQNEVTTSIER
                     DFVAQDGHILHTLVRTASEIDESGQSIGVLAMYLDITERKQAEEALRESETKLQTIFA
                     RSRDAIGVSKSGVHIFVNPAYVFMFGYESAEELIDTPVINLIAPESREMIIHNIQDRD
                     KGLAVPSDYETVALRKDGSTFILDVRISTYILQGEQYTLVILRDITERKRAEEALQIK
                     EWAIRSSINALALSDLDGSLIYVNPAFLALWGYQTEQEVLGRSAIEFWQVQENADQVI
                     SALRTQGGWMGEMVATRKDGTLLDVEVVASMVADNAGKPLCMMGAFVDITDRKRAEVE
                     LTIAKEKAEENEKKLREVLDNTKVQLWAFDGTRYNYVSKEWYDFTGQTLDTPLTIDLW
                     VSAVHPDDREPSGKIWLENWERKTAHDNYFRLRRQDGVYRDFYCHAIPIFDSQNKFKY
                     FHGFNVDITERKQAEEKLRHAENRLELFFSQSLDGFFFMLLDEPIQWSDDIDKDKTLE
                     HVFAHQRITKANDAMLSLFGTTRKQFIGLTPKDLYPGDVEQRKHFWGDLFDKGHLQLL
                     TAETKSDGSIMWLEGDYVCMYDTDGRLLGHFGIVRDVTERKQAEAVTAARARILQFSL
                     THSLPDFLQNVLDECELLTSSSIGFFHFMEADQNTLSLQNWSTRTLGEFCTAVGKHLH
                     YPLSQAGVWADCARERQAIICNDYAGAINSKGMPDGHAEIKRFVSIPILRGENVVAII
                     GIGNKAVDYDREDVEIVTQLADLSWDITERKRAEDSLWESETRFRRLANNAPDIIFRY
                     NFTPEMKLAFINPAVEKITFYTPEECYADPLLMLNMAHPDDAMMMAEYLQSRQTPDKP
                     LIMRWLDKYGTIHWMESRLVPIYDEADQLVAVEGITRDITERKRAELQIEEQLKRLNA
                     MHTIDTFIKSSFDLKNTLNILLTEVATQLKVDAASILLFNKDTLTLNYSAGYGFRSIA
                     IQHTKLRYGEGFAGRLLHDRKIIRIPDLTKTESELAKALALKGDNFTSYMGVPLIAKG
                     QTLGVLEIFQRAELTPDTDWFDFLEMLADQAAIAIDNMQLFESLQRSNLDLTLAYDAT
                     IEGWSRALDLRDKETEGHTQRVTSLTIQLAQQMGVPDADILHIRRGALLHDIGKMGVS
                     DNILRKPGPLTEAEWQEMRQHPLYAYKMLAPISYLNPALDIPYCHHEKWDGTGYPRGL
                     KGEEIPLVARIFAIADVYDAITSDRPYRAAWAKEKALDFIREQSGRHFDPNVVKYFLE
                     MIKDEVK"
     gene            5148..5465
                     /locus_tag="PLR93_02040"
     CDS             5148..5465
                     /locus_tag="PLR93_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNH25766.1"
                     /translation="MDEKTSSRVKIRVPKKELADFCRRYNVQKLALFGSVLGNNFRAD
                     SDVDVLVSFQPNAKIGFITFSRMQRELAELFNRPVDLVPMDGLKPVIRDSVLSDIEEV
                     YAS"
     gene            5455..5601
                     /locus_tag="PLR93_02045"
     CDS             5455..5601
                     /locus_tag="PLR93_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25767.1"
                     /translation="MRPEKLYLTDIVEAAQAIERFVMGESYYEFEQNEMMNSAVLQKI
                     DRDR"
     gene            5591..5800
                     /locus_tag="PLR93_02050"
     CDS             5591..5800
                     /locus_tag="PLR93_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNH25768.1"
                     /translation="MIGEAASRLPKEFTARFPEIPWVDIIGFRNIAVHEYFSIRWDIA
                     WIAATEEVPVLREQVEKILREEFAE"
     gene            complement(5895..6476)
                     /locus_tag="PLR93_02055"
     CDS             complement(5895..6476)
                     /locus_tag="PLR93_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25769.1"
                     /translation="MKYLSVALIALIMAGCGPSSPEMTATADMAMAETQTAAPTSTPT
                     LTPTSTATPTSTTTPILTLTPTLPHEAPGIDPGGMPESANSLFFESTPMFTFNLGGSV
                     KVNYIVFLEIKKELPKDAILEVHFQNPADPSTAYVVVVTDLTGEKIMVESKKFPMSNF
                     ECRNYWIDVHIYADRESIYELGTHVQWTSSSFC"
BASE COUNT         1819 a         1581 c         1788 g         1852 t
ORIGIN      
        1 atctgtctgg ttgaaattgt ctacctgatg gaaaaaggac gcatttcccc tgaaatgaaa
       61 tccatgttgg acgaggaatt gaaatcaggc gaaagcggac ttgtacttgc agacctcaat
      121 atggacgtag ttgaccagat tgccaacgtc ctgcgtgatg atattcctga cttgccagat
      181 cgggttattg cggcaaccgc acttgcatta aatttaccct tgattagccg tgacagaaaa
      241 attgttttga gtggtttgga tacgatttgg taatttggcg acgcaggtga agaatatctg
      301 ggtgccgacg ggggagtgac ccctgtcgct aactactctg tgaatagata gccctgcgcg
      361 aagcacgccc aacaaaagta cccttgtttc aaaataagag gtgccctatg ctaaaaacca
      421 tcaagcaatt ctttacgccg ccagtcttcc cagatgagga tgacaagacc cgtaatgcgc
      481 agatactcta tgcgctattt gctagcatga tggggatttt ggcacttctt atgcttgcca
      541 acatttttgt atttgttgcc aaagccattg tgagtatttt gctgtttatc cttttcattt
      601 ggatactggt tgcacgcgca ctggcgcaaa gcggcaaaat cctgcttgcc agccggatat
      661 ttgtgagcgg attgtggatt ctaactacat tccaactttt actgtctgga caactatctg
      721 ctgccgctct cgcttttcat gtgtcgaact tgtccattgc tggtgtcctt ttaaagaagc
      781 gttctgccat cacactggcg gtgatgagca gcctggttgg gttggggatg gtaattcttg
      841 aaatcacagg ttattcgttg catcgttact ttcccaaccc cccaattgta tattggatca
      901 cctgggtcat tgcttttatc atgaccatta tcccgctgaa cctgaccgtc cagagcgcag
      961 gagatgcgct ggcacgcgcg cagaagagcg aacgacgcta cgtgaccctg ttcaacaaag
     1021 cgccgatgat gtacttgata attcgcaatg agggagacat acccattatt tcggattgca
     1081 attttacatt cctaaaaaac ctgggctata cgcgtgaaca agtgatcggc cagccactgg
     1141 ctaatttcta tactccccaa tcacgcttga tgctattgaa agaaggtggt tttcagagtt
     1201 cgttccaaaa tgaggtgaca acgtcgattg agcgggattt tgtggcgcaa gatgggcata
     1261 tcctccacac actggtacgg actgcttctg agattgatga gtcggggcaa tccattggtg
     1321 tgctggcgat gtatctggac ataacagaac gcaaacaggc ggaagaggcg ctgcgcgaaa
     1381 gcgaaacgaa actacagact atctttgcaa gatcgaggga tgcaatcggg gtttccaaaa
     1441 gcggggttca tatttttgtc aatccggcgt atgtattcat gttcggatat gaaagcgcgg
     1501 aggagttgat tgacactccc gttatcaatc tcatcgcgcc tgaaagccgc gagatgatca
     1561 tccataatat tcaagatcgt gataaaggtt tagcagtgcc ttctgattat gaaaccgtcg
     1621 cgctccgaaa agacggatca acttttattt tggatgtgcg tatttccacc tacatattgc
     1681 aaggcgaaca atatacacta gtcattctgc gcgacatcac cgagcgcaag cgggcagagg
     1741 aggcgttgca aatcaaagag tgggctatcc ggtcttccat taatgccctt gccctatctg
     1801 acttggatgg gagcctgata tacgtcaacc ccgcttttct tgctttgtgg ggataccaga
     1861 ccgagcagga ggttctaggc aggtcggcta tagagttctg gcaggtacaa gaaaatgcgg
     1921 atcaagtcat ctcagcatta cgaactcaag gcggctggat gggcgagatg gtagccacgc
     1981 gaaaagatgg cacattgctt gatgttgaag tggtagccag catggttgca gacaatgccg
     2041 gcaaaccgct ttgcatgatg ggtgcatttg tcgacatcac ggaccgtaag cgggcggaag
     2101 tggaattaac cattgccaaa gagaaggcag aagaaaatga aaaaaagtta cgcgaggtgt
     2161 tggacaacac caaagttcaa ttgtgggctt ttgatggaac caggtataac tacgtgagca
     2221 aggaatggta cgatttcacc gggcaaacac tagacacccc gcttaccatt gatctgtggg
     2281 tttcggcagt acatcctgat gaccgcgaac cttcagggaa aatatggttg gaaaattggg
     2341 aaaggaaaac cgcgcacgat aattattttc gattgagaag acaagatggc gtatataggg
     2401 acttttattg ccatgccata ccgattttcg atagccaaaa taaattcaaa tactttcatg
     2461 gctttaatgt ggatatcact gaacgcaaac aagcggagga gaaattgcgc catgcggaaa
     2521 atcgccttga actttttttc tcacagtctc ttgatggctt tttcttcatg cttttagacg
     2581 agccgattca atggagtgat gatatcgata aagataaaac actggagcat gttttcgctc
     2641 atcagcgcat cacaaaagca aacgatgcca tgttgtctct gtttggaaca actcgtaagc
     2701 agttcattgg gctgacaccg aaagatcttt atcccggcga tgtcgaacaa aggaagcatt
     2761 tttggggtga tctttttgat aaaggacatc ttcagctgct taccgctgag accaagtctg
     2821 atggttccat catgtggctt gaaggggatt atgtgtgcat gtatgatacc gatggacgtc
     2881 ttttggggca ttttggcatc gtacgcgatg tcaccgaacg caagcaggca gaggcggtta
     2941 ctgccgcacg cgcgcgcatc ttgcaatttt cactgacaca ttcgcttcca gatttcttgc
     3001 aaaacgtcct cgatgaatgt gaattgctca catccagttc gatcggtttt ttccatttca
     3061 tggaagccga ccagaataca ttgtcgctac aaaactggtc cacacgcact ttgggtgagt
     3121 tttgtactgc tgtaggcaaa cacctgcatt atccattaag tcaggcggga gtctgggctg
     3181 attgcgcccg tgaacgacaa gccatcattt gcaatgatta tgccggggcg ataaacagca
     3241 aaggcatgcc agacgggcat gcggagatca aacgttttgt gtcgattccc atcctgcgcg
     3301 gcgaaaacgt tgtagccatc attggcattg gtaacaaagc cgtggactat gatagggagg
     3361 atgtcgagat tgtgacccaa ttggcagacc ttagctggga tattaccgag cgcaaacggg
     3421 cagaagattc gttgtgggag agcgaaactc gtttccgacg gttggccaat aacgcccccg
     3481 atattatttt ccgttataac tttaccccag agatgaagct ggcttttatt aacccggcag
     3541 tggaaaaaat caccttctac accccggagg aatgttatgc agacccgctg ttaatgctca
     3601 atatggcgca tcccgatgat gcaatgatga tggctgagta tttgcaatcg cgccaaactc
     3661 ctgacaagcc tttgatcatg cgctggctgg acaaatacgg aacaattcac tggatggaaa
     3721 gtcgtcttgt gccgatttac gatgaagcgg atcaacttgt ggcggtggaa ggtatcaccc
     3781 gtgacattac tgaacgaaaa cgggcagaac tacagatcga ggaacaactc aagcggctga
     3841 acgccatgca caccattgat actttcatca aatccagttt tgacttgaag aacacgctca
     3901 atattttact gactgaggtt gccacccagc tcaaggtgga tgccgcctcc atacttttat
     3961 tcaacaagga tacacttacg cttaactact ccgccggcta tgggttccga tctatagcca
     4021 tacagcacac aaaacttaga tacggcgaag gctttgcggg ccgcctcctt catgacagga
     4081 aaatcatccg cattccagac ctgacgaaaa cagagagcga acttgccaaa gcccttgcac
     4141 tgaaaggtga taattttact tcttacatgg gcgtgccgct gattgccaag gggcaaaccc
     4201 tgggtgtact ggaaatcttc cagcgcgctg aactcacccc agacactgac tggtttgact
     4261 tccttgaaat gcttgcagat caggcagcca ttgcaattga caacatgcaa ttattcgaaa
     4321 gcctgcaacg ctccaatctt gaccttacct tggcttacga tgccactatc gaaggctggt
     4381 cgcgcgcatt ggacctgcgc gacaaggaga ccgaggggca tacccagcgt gttacatcct
     4441 tgaccattca actggcacag caaatgggcg ttcccgacgc cgatattctt cacatccgcc
     4501 gcggtgcatt gctccacgat attggcaaaa tgggtgtgtc ggataatatc ctgcgtaagc
     4561 ctggtccatt gaccgaggcg gaatggcagg aaatgcgcca acatcctcta tatgcgtata
     4621 aaatgctcgc ccccatctct tacttgaacc ccgcactgga tatcccctat tgtcaccacg
     4681 aaaaatggga tggcacaggg tatccgcgcg gtttgaaagg cgaagaaatt ccactagtgg
     4741 cgcgtatctt tgccattgcg gatgtgtacg atgccatcac tagtgaccgt ccttatcgtg
     4801 ccgcgtgggc aaaggaaaaa gctctggatt ttatccgtga gcaaagcggc aggcactttg
     4861 accccaatgt tgtcaagtat ttcttggaaa tgataaagga tgaagtgaaa taaactttta
     4921 tttgcaaaat agttatatgt gtttacatca aacctgattg ctcagaggaa ggtagttaca
     4981 tttcgagaat aagtctactg cgaacataaa gcgcacatgg acaggctacg gtctgccccg
     5041 cgattggctc gaccgcgagc aaggcgaagg tcacgagttc ctgcacgagg caactcaggt
     5101 caagaatatc tgggtgccga cgggggagtg acaggcataa ttaaggcatg gacgagaaaa
     5161 cttcttcccg cgtgaagatc agagttccca agaaagaatt ggctgatttc tgccgccgtt
     5221 acaacgtgca gaaattggct ttattcggtt ctgtgcttgg gaataacttc cgcgcggata
     5281 gtgatgtgga tgtgctggtg tcctttcaac ccaatgcaaa aatcgggttc atcactttct
     5341 ctcgaatgca gcgcgaactt gccgagttgt tcaatcgccc cgtcgacttg gttcccatgg
     5401 acggtttgaa gcccgtcata cgcgactctg tcctctcaga tatcgaggaa gtgtatgcgt
     5461 cctgagaaac tttacctgac ggatatcgtt gaagccgccc aagccattga gcgctttgtc
     5521 atgggcgaga gttattatga gttcgagcaa aatgagatga tgaatagcgc tgtgctccaa
     5581 aaaattgacc gtgatcggtg aagccgcatc ccgtttgccg aaagaattta ctgctcgatt
     5641 tcctgaaatc ccgtgggtgg atattatcgg ttttcgcaat atcgccgtcc atgagtattt
     5701 ctccattcgt tgggatattg catggattgc ggcgacagag gaagtccctg tgttgaggga
     5761 acaagtcgag aaaattttgc gggaagagtt tgcagaataa gaatatctgg gtgccaacgg
     5821 gggagtaggg aagagcaggg aagtagagat tgaagagtag atgacctctg aggctggcct
     5881 cagaggtctg ttttttagca aaaactggaa cttgtccatt ggacatgtgt gcctaattcg
     5941 tagatcgatt ccctgtctgc atagatgtgg acatcgatcc agtagttcct gcattcgaaa
     6001 ttggacatgg ggaatttctt ggattcgacc attatctttt cacccgttag gtctgttacg
     6061 accacaacat aggccgttga cgggtcagct gggttttgga aatggacctc gagtattgca
     6121 tccttgggta attccttttt tatctcaaga aaaacaatat aattaacctt tacagaaccg
     6181 ccaaggttga atgtaaacat gggcgtgctt tcaaagaaca aagagtttgc agattctggc
     6241 atgccacccg ggtcaatgcc gggcgcttca tgtggaagtg tgggagtcaa ggtcaatatc
     6301 ggggtcgtgg ttgacgttgg ggttgcggtt gatgtcggag tcaaagtagg cgtggatgtg
     6361 ggggctgcgg tttgagtttc cgccattgcc atatcggcag tggctgtcat ctcaggcgat
     6421 gacgggccgc acccagccat gatcaaagcg attaatgcta cggataaata tttcatggta
     6481 attctcctgt tggctattcc gtttgatgcg attgttttga ttatggcgtt atacaacctt
     6541 tccccggctt ttaactgcca ataaccgcca atttttaatt ttggcctgcg taatagaatt
     6601 ttcccctgtt ctcatccatc ttgccgtgtg gggacgcctc cagaccgggt ttgaccaggg
     6661 acaggatggg acagaatttc gaggcgcttt cgaggccttt ttttgtgaaa aatgggctgt
     6721 tttgagtgga aaaacttctt gaagttttta aaaaacattt agaagtcttt gggaaaacat
     6781 ctagaagttt ttaaaaaact tatagatgtt ttttaaaacg agtcaaggga ggtttctgat
     6841 cctctcaagg aagggtttaa ttccttccct gactcgtttc caatcctccc ctgagaggac
     6901 ttcttgttcc ccctgtttcg atttgcgtat aggcaatcgg atgttatgct tgctaggcag
     6961 cggatttggg tcggttacgg ctcgtcccgc gattggctcg accgcgagca aggcgaaggt
     7021 cacgagttcc tgcacgaggc
//
LOCUS       DAOQFT010000046         7006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5247_length_7006_cov_6.027766, whole genome shotgun sequence.
ACCESSION   DAOQFT010000046 DAOQFT010000000
VERSION     DAOQFT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5247_length_7006_cov_6.027766"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2288
                     /gene="recJ"
                     /locus_tag="PLR93_02060"
     CDS             <1..2288
                     /gene="recJ"
                     /locus_tag="PLR93_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNH25770.1"
                     /translation="MTRWLDPQPVDIPASFQDLGLPPLIAQTLLRRGISSPVEAEAFL
                     YPEKNPSSQFPNIEKAVELLEMAIRNREKICVWGDFDVDGQTSTALLVQTLRDLNADV
                     MYYVPVRGKESHGIHIESLKPIIDNGAKLLLTCDTGITAHDAIDYANSRGLTVIVTDH
                     HDLGETLPNAQAIINPKLLPEDHPLRNLAGVGVAYKLAEALLDTRTSNIDSQISNIEL
                     LDLVALGLIADVALLQNETRSLAKQGIEQLRNTSRLGLRVMAELAGAAFETLTEETIG
                     FTFAPRLNALGRLGDANPAIDLLITDDSARARVLATQIEGLNAQRRMLTKQVNDAAEA
                     QLKENPELLNHPIIILSHPNWPGGVVGIVANKLVERYHKPAILFNESEDGILRGSARS
                     IEGLHITEAITTQKDLLIGFGGHPMAAGMALHKENLPAFRKGLGKAIEKQLGDIVFEE
                     PTLQIDAWLGLTDLSLDLADSLELLAPFGAGNPQLTLATRNVMLKSAISLGKTKEHLR
                     LNVEDENGEVASFLWWGGAGEELPPKDSKFDIAYTLRATSFRGQRQVTLQFKEFRVTE
                     ERPIEVKEAKLDIRDMRLQHTLVNAQPSALIWAEGPDRAIGVNRLELTQAKELTIYTT
                     PPSSIELRKALDIVQPEVVYFFSISPAEQSPEEFLTYLAGLCKFVLNQRHGKTTISEL
                     AAASAARESAIQLGLEWLAAGGQLTVTLEENEVQLSKETQEKNPYLQMELFIALRGVL
                     SETSAYRKYFATVPDLKTVFR"
     gene            2299..2742
                     /locus_tag="PLR93_02065"
     CDS             2299..2742
                     /locus_tag="PLR93_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HNH25771.1"
                     /translation="MTNRVLFICTGNYYRSRYAEIYFNTIAQNLSIDWEADSCGLDTS
                     GGHNVGPISKFALERLKIRNISPDPLRYPRQLEEKDLQEASLIIAINRVEHQPMIERQ
                     FPAWADRITYWEIADLNITSADDALSALESNVEKLVANLATSHNQ"
     gene            complement(2723..3229)
                     /locus_tag="PLR93_02070"
     CDS             complement(2723..3229)
                     /locus_tag="PLR93_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25772.1"
                     /translation="MTDSPANLLCKACGLCCSGHLFSWVRLDANELDPIAKLGIDVIR
                     DDPRQRGFLQPCPMWNGVCTIYTIPEYPRSCKRYKCKVFRQLVDDDISFPAAMSIIQE
                     TLTLIREIEPLLPVSEIPSFRERLIAHKEELEAKKEVDNDFMQKTLTLLTRYEDLFGV
                     DDFIDYDS"
     gene            complement(3324..4478)
                     /locus_tag="PLR93_02075"
     CDS             complement(3324..4478)
                     /locus_tag="PLR93_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNH25773.1"
                     /translation="MTDLNQAIQLIRQGQKFEAQRILQEIIKTEPKNIPAWFWYVETC
                     ATTERRIQVLETCLKMNPGNLQVEQALQIFRNSQPPVPAPPKVEPPTYQSNTPEPQKN
                     DDLYGYTSFRSDSFGQTQSYEEDKSPAYDSPLYEYETFRTNSSVKEESQKKQPWEMDL
                     STYEDNSMLAKSKKVRRSYSAFDVWLTALTSQDEKSYADLLEDPEAGLERAFSWVAIA
                     GLITALTVPVQYFLNPNLGELLDIPELQRQVGNMTPNAFIIILTMISFVTAPIGAILN
                     LALSGGIQNLLARFFGGGGNFKRTAYALSAFLAPMAVVTSLVAIVPVAGGCLSFPLGI
                     YSFILNVRALKASHSLTNGAAIGVLLAPSILVIIFGCLFLFMSGTSLSSS"
     gene            complement(4550..6382)
                     /locus_tag="PLR93_02080"
     CDS             complement(4550..6382)
                     /locus_tag="PLR93_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNH25774.1"
                     /translation="MYGWHLRILRVNLTTRKVTTEDVDPQIARDYLGGRGWAIHYMYK
                     EMDPAVDPLSPENMLIFATGPLTATPAPTGNRYMVVTKSPLTGALAHSNSGGEFPTWM
                     KRTGFDMFIFEGKASEPVYVLVNEDQIEIKSAAHVWGKDTHETTDILKAETSDEARVA
                     CIGPAGENLALMAAIMNDKHRAAARSGVGAVMGSKNLKAVVAMGNKNPPLHDSEGMRA
                     ISVETSKNVGADVKKGSNMRIYGTSYVPQVTNTLGILPTRNFLQGTFEDVHNVDGDAL
                     KNQYLIRHTPCYRCPLSCGRLTEVPDGPYKGKGEGPEYETISSLGTGCGVSNLAALVK
                     ANYLCNEYGMDTITTGMTIAVAMEMYEKGYLTEETIGMPLKFGDHDAMIEMIKLMAYN
                     KGFGQDLVQGSYRLAEKYGHPELAVTTRKQEFPGYDPRGSQGMGLLYATSNKGASHME
                     GDVAYEEVFGVPIKENPLSTEGKPELVKHFQDSFALIDSSGLCVFVAIRYVFDKNRLI
                     WPQTLAAMMNLSTGAGYTPEEVMKAADRVYTLERMFLIKAGSGPEWDVLPYRMLHEPL
                     PDGPAKGKVAELDKMLPEFYEKRGWDEKGYPTQAKLMELGLPLN"
     gene            complement(6438..6677)
                     /locus_tag="PLR93_02085"
     CDS             complement(6438..6677)
                     /locus_tag="PLR93_02085"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNH25775.1"
                     /translation="MLIRVKMIANYRDALPPGHKHGVIELDVPEETTVYDVISRFDIP
                     LNDESVIVLNGLTVDMDTPLREGDMVTAFSAIAGG"
     gene            6814..>7006
                     /locus_tag="PLR93_02090"
     CDS             6814..>7006
                     /locus_tag="PLR93_02090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH25776.1"
                     /translation="MFVEEDGRLTSEFTLNEAHQGPPGHAHGGASAAILDEVMGLVVW
                     AAGHQVLAANININYRKPLP"
BASE COUNT         1899 a         1723 c         1636 g         1748 t
ORIGIN      
        1 ttatgactcg ctggctcgac cctcaacctg tagacatccc cgcctctttc caagacttgg
       61 gcttgcctcc cctcatcgcg cagactctcc tccgacgcgg aatcagctcc cccgtcgaag
      121 cggaggcgtt tctttatccc gaaaagaatc ccagttctca atttccaaat atcgaaaaag
      181 cagtcgaact ccttgaaatg gctatccgca atagagagaa aatctgcgtc tggggtgatt
      241 ttgacgtaga cgggcaaacc tccacagccc tgcttgtgca aacgttgcga gatctgaatg
      301 cagacgtgat gtattacgtg cccgtgcgcg gaaaagaaag ccacggcatt cacatcgagt
      361 cgcttaaacc aattattgat aacggcgcaa agctactgct cacttgcgat accggcatca
      421 ctgctcatga tgccattgat tacgccaact ctcgcggact aacagtcatc gttacagacc
      481 atcatgattt gggtgaaacc ctgccaaatg cacaagccat catcaacccc aagcttctgc
      541 cagaagatca tccactgcga aatttggcgg gtgttggggt tgcgtacaaa ctagctgaag
      601 cgttgctcga tactcgaaca tcgaacatcg actctcaaat atcgaatatc gaattactcg
      661 atttagtagc tcttggcttg atagccgatg ttgccttgct tcaaaacgaa actcgctcac
      721 ttgccaaaca aggcatagag caactaagaa atacaagtcg ccttggtttg cgtgtgatgg
      781 cagaactggc tggcgccgca tttgaaacac tcaccgaaga aacgattggc tttacctttg
      841 ctccacgtct caacgcactc ggtcgtttgg gtgatgccaa ccccgccatt gacttattaa
      901 ttacagatga ctctgcacgt gcgcgagtgc ttgccactca aattgaaggg ctaaacgctc
      961 aacgccgaat gctcaccaag caagtcaacg atgcggcgga ggcacaacta aaagaaaacc
     1021 ccgaactgct taatcatccc atcatcattc tttcgcatcc taactggccc ggtggtgtgg
     1081 tgggcatcgt tgccaataaa ctcgttgagc ggtatcacaa acccgcaatt cttttcaacg
     1141 agtctgaaga tggcatcttg cgcggctcgg ctcgttctat tgaagggttg cacattaccg
     1201 aagccatcac cacacagaaa gacttgctta tcggcttcgg tgggcatccc atggcagcag
     1261 gcatggcatt acacaaagaa aatctgcctg ccttccgcaa gggacttggc aaagcgattg
     1321 aaaaacaact tggggatatt gtctttgaag aacccacgct tcagatcgac gcctggctgg
     1381 gtctcaccga cttgagcctt gacctcgccg atagcttgga gctactcgct cctttcggcg
     1441 ccggcaaccc acagctgacc ctcgccacgc gcaatgtcat gctcaagtct gccatttcgc
     1501 ttgggaagac caaagaacat cttcgcctaa acgtagaaga tgaaaacggt gaggttgcca
     1561 gtttcttatg gtggggcggc gccggagaag aacttccacc caaagacagt aaattcgata
     1621 ttgcctacac tttacgtgcc acatccttcc gtgggcaacg tcaagttacg ctgcaattta
     1681 aagaatttcg agttactgaa gaacgcccta ttgaagtaaa agaagcaaag ctcgatattc
     1741 gagatatgcg tttgcaacat actttggtta acgcacaacc atccgccctc atttgggcag
     1801 aaggacccga tcgcgcaatc ggagtcaacc gtcttgaatt gactcaagct aaagaattga
     1861 ccatctatac gacgccgcct tcttctattg aactacgaaa agccttggat attgtccaac
     1921 cagaggttgt ttacttcttt agtatttccc ctgccgagca aagccctgaa gaattcctca
     1981 cgtatctagc agggttatgc aaattcgtcc ttaatcaacg ccatgggaaa accaccattt
     2041 ctgaactcgc cgccgcatcc gccgcacgag agtctgccat tcaactcggt ttggaatggc
     2101 tggcagccgg cggtcaattg accgtgactc ttgaagaaaa tgaagtccag ctttcaaaag
     2161 agacacaaga aaaaaatccg tatctacaga tggaactttt cattgcgcta cgtggcgtct
     2221 taagtgaaac ctctgcatac cgaaaatatt tcgcgacagt tccagaccta aaaactgttt
     2281 ttagataaaa aaagcaaaat gacaaatagg gttttattta tttgtaccgg caactactat
     2341 cgcagccgat atgcagaaat ttatttcaat acaatagctc aaaacttaag tattgattgg
     2401 gaagctgact catgcgggct ggatacatca ggtggacaca atgtgggtcc gatctcgaaa
     2461 tttgcgttgg aacgcctcaa aatccgcaat atctcacctg accctttgcg gtatccgcga
     2521 caattggaag aaaaagactt gcaagaagca agccttatca ttgcgatcaa tcgagtggaa
     2581 catcagccaa tgattgaaag acagttccca gcatgggctg accgtatcac gtattgggaa
     2641 atagcagact taaatatcac cagtgcggac gatgcactat ctgcccttga gagcaacgtt
     2701 gaaaaactcg tagcaaacct tgctacgagt cataatcaat aaaatcatct acaccaaaca
     2761 gatcttcgta gcgggttaac aaggtcaaag ttttttgcat aaaatcgtta tctacttctt
     2821 tcttcgcttc caattcttct ttatgtgcga tgaggcgctc tcgaaaactg gggatttctg
     2881 acacaggtaa aagcggctca atttcgcgga tcaaggtcag cgtctcttga atgatagaca
     2941 tagcagccgg gaaagaaata tcatcgtcca caagttgacg gaacactttg catttataac
     3001 gcttgcaact acgggggtat tcagggatag tgtagattgt gcagacccca ttccacatgg
     3061 ggcaaggttg caaaaatccg cgttgacgtg gatcatcacg gatcacgtca atgcccagtt
     3121 tggctatcgg atcgagttca tttgcatcga gccgaaccca cgagaaaaga tgccctgaac
     3181 agcatagtcc acaggcttta cagagaaggt ttgctggaga gtcggtcatg tatggatgat
     3241 aacaaaggta cgagaaactg aatcaactat cccttaagaa agaagcgggc gcatttggaa
     3301 gcatccgctt ctttttggat atcttaagag gacgataatg aagtccctga cataaaaaga
     3361 aataaacacc caaatataat aaccagaata gatggtgcta ataagacgcc aatggcagca
     3421 ccgtttgtca aagagtgaga agccttcaag gcgcggacgt tcagaataaa actataaatg
     3481 ccgagcggaa aagaaaggca accgcccgca accggcacaa ttgccacaag ggaagtaacc
     3541 actgccattg gtgcaaggaa tgcagataat gcatacgctg ttcgtttaaa gttgccgcca
     3601 ccaccgaaga accgcgccaa caaattttgg atgccccctg aaagtgcaag attaagaatc
     3661 gctccaatag gcgccgtgac aaaagagatc atggtcagaa taataatgaa ggcattgggc
     3721 gtcatattcc caacttgccg ttgcaattcg ggaatatcaa gaagctctcc taaattaggg
     3781 ttgagaaagt attggacggg aactgtcaaa gccgttatta acccggcaat ggcgacccaa
     3841 ctaaaagcac gttcaagacc tgcttctggg tcttccaata ggtcggcata tgatttttca
     3901 tcctgagagg ttaaagctgt caaccataca tcaaacgcag aataactacg ccgaaccttt
     3961 ttagacttgg ctaacatgga gttatcttcg taggtagaca aatccatttc ccaaggttgc
     4021 ttcttctgag actcttcttt cactgatgaa tttgtgcgaa atgtttcgta ctcatacaag
     4081 ggcgaatcat atgctggaga tttatcttct tcataagatt gtgtctgccc aaaagaatcg
     4141 gaacggaatg aggtgtaacc gtacaaatcg tcgttctttt gaggttcagg agtgttgctt
     4201 tgataagtgg gcggttcgac cttgggagga gctggtacgg gcggttgaga gtttcgaaaa
     4261 atctgtaacg cctgctctac ttgaaggtta ccagggttca tttttagaca agtttcaagg
     4321 acttgaatcc gtctttcagt ggtcgcgcag gtttccacat accaaaacca agcagggatg
     4381 ttcttcggct cggtcttgat aatttcttgc agaatacgct gcgcctcaaa tttttgcccc
     4441 tgacgaatga gttggatggc ttgatttaga tctgtcattc gaactcccaa gaaaaagata
     4501 tgcccatagc ttgtgttgac caagctatgg gcaggttaaa acaactctgc taatttagcg
     4561 gcaaacccaa ttccattaac tttgcctgag tggggtaacc tttttcatcc cagccacgtt
     4621 tttcatagaa ctctggcaac atcttgtcga gttctgctac ttttcccttg gcaggaccat
     4681 caggcaaagg ttcatgcaac atgcggtaag ggagcacatc ccattcggga ccagaccctg
     4741 ctttgatgag gaacatgcgc tcgagggtgt aaacacggtc ggcggctttc atcacttctt
     4801 caggggtata gcctgcgccg gtggaaaggt tcatcatggc agccaaggtc tgaggccaga
     4861 taaggcggtt cttgtcgaat acatagcgaa tggcaacgaa tacgcacaac cctgaggagt
     4921 cgatcaaggc aaacgaatct tggaagtgtt tgaccagttc tggttttcct tccgttgaaa
     4981 gcggattttc cttgatcggc acaccaaaga cttcttcata cgccacatcc ccttccatgt
     5041 gtgatgcgcc cttgttggag gtggcataca acaagcccat gccttgcgag ccacgtggat
     5101 catagccggg gaattcttgc ttacgagtcg tcactgccaa ctcggggtga ccgtactttt
     5161 ccgcaaggcg ataactacct tgcaccagat cctgaccaaa gcctttgtta taagccatca
     5221 gcttgatcat ctcgatcatg gcatcatggt cgccgaactt aagcggcata ccaatagttt
     5281 cttcggtgag atagcccttt tcgtacattt ccattgccac agcaattgtc ataccggtgg
     5341 tgatggtatc catgccgtat tcgttgcaca gataatttgc tttgaccaat gcggcaagat
     5401 tgctcacgcc gcaccccgta cctaaagatg aaatggtttc atattcagga ccttctccct
     5461 tgcctttata cggaccatca ggcacttcgg tgaggcgccc acatgaaagc ggacagcgat
     5521 aacaaggggt atggcgaatc aaatattgat tcttcaaggc atcgccatct acattgtgaa
     5581 catcttcaaa agtgccttgt aaaaagtttc gcgtgggcaa aatgccgagg gtgttggtca
     5641 cttgtggcac atacgaagtg ccataaatac gcatgttcga gcccttcttt acgtcggcac
     5701 caacattctt ggaggtctcc acgctgatgg cacgcatccc ttcagaatca tgcagaggcg
     5761 ggtttttatt tcccattgca actacggctt tgagattctt acttcccatc acagcaccca
     5821 cacccgaacg agccgccgca cgatgtttat cgttcatgat ggctgccatc agtgcaagat
     5881 tctcgcctgc aggtccgata catgcaactc gcgcttcatc agatgtttct gccttgagga
     5941 tgtccgtcgt ttcgtgtgta tccttgcccc acacatgagc cgcggactta atttcgattt
     6001 ggtcttcgtt caccaaaaca taaactggct ctgaggcttt gccctcaaag ataaacatat
     6061 cgaaccctgt gcgtttcatc cacgttggga attcaccacc agagttagag tgtgcaagtg
     6121 cgcccgtcag cggagatttg gttaccacca tgtatcggtt gccggtcggg gcaggcgtgg
     6181 ccgttagcgg accagtggca aagatgagca tgttctctgg cgaaagcggg tccacggcag
     6241 ggtccatttc tttgtacata taatggatcg cccatccgcg cccaccgaga tagtcgcgtg
     6301 cgatttgtgg gtctacatct tcagtggtta cttttcgagt agtgaggttt acgcgtaaga
     6361 ttctgaggtg ccagccgtac atgggaactc cttgaaaaag tttgaacgtt gaaatgtttc
     6421 aacgattaga accgagatta gccgccagcg attgcggaaa atgctgtgac catgtcaccc
     6481 tctcttaacg gggtatccat atccacggtg agaccgttca agacgatcac gctttcatca
     6541 ttaaggggaa tatcaaaccg agagataaca tcgtagaccg ttgtctcttc ggggacatcc
     6601 aactcaatga cgccatgttt atgccctgga gggagggcgt cacgatagtt ggcaatcatc
     6661 ttgacacgga tcagcataag gcgattctag cataatcagc caaagggtac agaaggtctt
     6721 tgtggttaaa ttgacgttat gagaaaaaaa gtacctaacc atggtccctg ctttgtatgc
     6781 gggagtgaaa acccgcaatc cattggaatt acgatgtttg ttgaagagga tggaagacta
     6841 acatctgaat tcaccttgaa tgaggcacat caaggtccgc cggggcatgc ccatggcgga
     6901 gcctcggcag ccatcttaga cgaggtaatg ggcttggtcg tttgggcggc ggggcaccaa
     6961 gtgctggcag caaacatcaa tatcaattat cgcaaaccgc ttccgc
//
LOCUS       DAOQFT010000047         6794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5486_length_6794_cov_6.863184, whole genome shotgun sequence.
ACCESSION   DAOQFT010000047 DAOQFT010000000
VERSION     DAOQFT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5486_length_6794_cov_6.863184"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLR93_02095"
     CDS             complement(<1..524)
                     /locus_tag="PLR93_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNH25777.1"
                     /translation="MNPASLTLAEQRELIKSKEISASDLAAACYRQIDRLNPTLNAFI
                     TVIPQGEEETPDNRHIALLQGIPIAVKDLYNTKGIRTTGGSKFFADYVPSEDAFVVDK
                     IKKAGAHIIGKTNTHEIALGVTNNNPHFGACRNPWDIARTPGGSSGGSAVAVATGMAL
                     AAIGTDTGGSIRIPA"
     gene            complement(633..2639)
                     /locus_tag="PLR93_02100"
     CDS             complement(633..2639)
                     /locus_tag="PLR93_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNH25778.1"
                     /translation="MATKGKSVKKGAKAAPKKVAKKVVAKKKAAPKPSNTFQPTKIKL
                     LRPVPSDIDIAQAGKLKPITQIAKELGIRDDELELFGPYKAKIKLEILERLKNKPNGK
                     YIDVTAITPTPLGEGKTTTTVGLSQALGAHLGKKVITAIRQPSMGPTFGIKGGAAGGG
                     YSQIIPMEDFNLHLTGDIHAITASHNLVAAALDARVMHEKEQDDEKLFNALCPPNKKG
                     QRKFSPTMLRRLEKLGIKKTNPDDLTPEERSRFARLDIDERTITWRRVVDINDRMLRE
                     VEVGRGKDEVGHAHMSGYDITVASEIMAVLALTTGIEDMRERFGKMVVATSKRGDAVT
                     VEDLGVAGAVTVLMKDAIKPNLMQTLEGTPATVHAGPFANIAHGQSSIIADKIALKLV
                     GKDGFVVTESGFGADIGMEKFFNIKCRYSGLIPHVVVMVATVRALKMHGGGPKVVAGK
                     PLAPEYTDENLELLKAGLVNLERHIQNAMKFGVKVIVAINSFATDTEAEVELIRKSAI
                     EFGAMDAIKCTHWADGGKGTKKLAEAVVKAAEQPSNFKFLYDPKLPIKEKIEIIAKEI
                     YRADGVDYSPEAEEQVERYTRLGFNDLPICMAKTHLSFTTDATKKGAPTGFRITVREV
                     RASVGAGFLYPILGDMRTMPGLPTRPSFYDVDLDMETGKVLGLF"
     gene            complement(2735..3535)
                     /locus_tag="PLR93_02105"
     CDS             complement(2735..3535)
                     /locus_tag="PLR93_02105"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNH25779.1"
                     /translation="MTNELVLLKLGGSLITDKDTPYTPRLDKLANLAKEIKTALDSTP
                     GLKLILGHGSGSFGHVAAKKHGTRDGVQTKEQWLGFTEVRLQAAELNRYVVTSLFEAG
                     IPALPFPPSASMVSDKRKVTHHNVETIRRALGVDLLPVVQGDVAFDETLGGTILSTED
                     VFTFLVDQFPTTRILLAGIEAGVWADFPARTKLVKQIQLSDYEAMRSGIKGSVSTDVT
                     GGMKAKVEEMLSLIQKKPGLTVQIFSAEEPGFLTRALQGENVGTLLTA"
     gene            3602..4447
                     /locus_tag="PLR93_02110"
     CDS             3602..4447
                     /locus_tag="PLR93_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25780.1"
                     /translation="MDIRTGVFVLALFAAVGAFLSFRGAIRNMQAARKISFYSLRKRY
                     NNTAWRLVFLAFILIGVAFWFPSGGAQVMYQVFPPSVTPSLTPTITLTPTITLTPTIT
                     LTPTLTPTPLVSDTPTLTPTPFLPAAIEALFAGPVTPNPDSIFTAIQFSTEFDGVNAV
                     DPKTVFTLPIDTIYGGFDYNNTQPGVQWTALWFRNGELVCYETEPWREEWGTGGIGGY
                     TSCSSPLDGWQAGQYEVQIFMGYDWKVVGRFVVMDSLTPSVTVTGTPDLTQIGTPTVS
                     PTATP"
     gene            4471..4962
                     /locus_tag="PLR93_02115"
     CDS             4471..4962
                     /locus_tag="PLR93_02115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH25781.1"
                     /translation="MTEKAFQEYYPEHLSHCYGCGTMNELGHQIKSYWDGEESVCHFQ
                     PKPYHTAIPGYVYGGLLASLIDCHGTGTAAAAGYRAENRSMDTEPALRYLTASLHVDY
                     LKPTPMGPVLEIRARVKEVKGRKVVVEEWIQVNGVVTVKGEVVAVQVPEALVNELLEK
                     GAN"
     gene            4986..5504
                     /locus_tag="PLR93_02120"
     CDS             4986..5504
                     /locus_tag="PLR93_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25782.1"
                     /translation="MKKVWIGILLGVVLAGCSTNVPPEMRARMMDTENVPSEWIIFNE
                     SKGEDWGGQLYTVAFAYGNESESPALEQQLIVYSDESAAITGYDEFDAYIFVEEWTVN
                     PEITFSPSTATDKFAAKCTDREIDHVMTKICFVLQQHANYVSALGVHMGGPITFDVLN
                     SVLKSIDTKLNQ"
     gene            complement(5547..5972)
                     /locus_tag="PLR93_02125"
     CDS             complement(5547..5972)
                     /locus_tag="PLR93_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HNH25783.1"
                     /translation="MSITLEQKKLVQETWEKVVPIAETAAELFYGRLFEIDPELKPLF
                     KNADMKEQKMKLLQMLGMAVKGLDNLDQLVPAVENLGRRHVGYGVKDSHYDTVGDALL
                     WTLEKGLGEDFTPEVMEAWTETYVTLATVMKNATETVAA"
     gene            complement(6123..6512)
                     /locus_tag="PLR93_02130"
     CDS             complement(6123..6512)
                     /locus_tag="PLR93_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH25784.1"
                     /translation="MKNEFVLSETFNANAAAIYNAWLSTQGHTLMTGSPAKVNGHAKG
                     DFTAWDGYIWGTFLDLEPNKRIVQLWRTGEFPDDAEDSRVEILLEETNGKTKLTLRHT
                     HMPEDQIESYKTGWLDFYFKPMKEYFG"
     gene            complement(6550..>6794)
                     /locus_tag="PLR93_02135"
     CDS             complement(6550..>6794)
                     /locus_tag="PLR93_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNH25785.1"
                     /translation="VPEADAISKSEMEPMIAQASAEAVEKGIHGQALTPFLLGRISEL
                     TKGKSLKTNLALLLNNARLAGEIANQMYTRKKEWAI"
BASE COUNT         1630 a         1591 c         1673 g         1900 t
ORIGIN      
        1 gctggaatgc gaatggaacc gcctgtatca gtgcctatag cagcaagtgc catgcctgtt
       61 gccactgcaa ctgcgctccc gcctgatgag ccgcccgggg tgcgggcaat atcccaaggg
      121 ttgcggcacg ccccaaaatg cggattgtta ttggttacac caagagcgat ctcatgggta
      181 ttggttttgc caataatatg cgctcctgct tttttgatct tatccacaac gaatgcatct
      241 tcactaggca catagtctgc gaagaattta gagccacccg ttgtgcggat gcctttggta
      301 ttgtataaat ctttaactgc aatcggaatg ccctgtaaaa gagcgatgtg tcggttatca
      361 ggcgtttctt cttcaccttg tggaatgaca gtgatgaagg cattaagcgt cgggttgaga
      421 cggtcaattt gacggtaaca agctgcggcg aggtcagaag cgctgatttc cttacttttg
      481 atcagttctc tttgttcagc taaagtcagg gaagcgggat tcataagggt gaattatatt
      541 caatttatac ttattttcaa tatagcctat cggtactgaa aaaacaggtc acgcctaacg
      601 cgtgacctgc atttttgtag gtagagattg ttctagaaca acccgaggac cttgcctgtt
      661 tccatatcca agtccacatc gtagaaagat gggcgagtgg gcaaaccggg catggtgcgc
      721 atgtcgccga ggattgggta caagaagcct gcgccaacgc tggcacgcac ttcgcggacg
      781 gtgatacgga agcccgtagg cgcacctttc ttggtggcgt cggtagtaaa ggaaagatgg
      841 gttttcgcca tgcagatcgg aaggtcgttg aacccgaggc gggtatagcg ttcgacctgt
      901 tcttcggctt caggcgagta atccacacca tcagcgcgat agatttcctt ggcgatgatc
      961 tcgatttttt ccttgatggg aagcttcgga tcatacagga acttgaagtt gctcggctgt
     1021 tctgcagctt taaccaccgc ctcagccaat ttcttagtgc ccttaccgcc atcagcccag
     1081 tgcgtacatt tgatggcatc catggcgccg aactcaatgg cagatttgcg gatgagttca
     1141 acctcagcct ctgtatctgt cgcaaaggag ttgatagcaa caataacttt gacaccgaat
     1201 ttcattgcat tctgaatatg gcgttcaagg ttaacgagac ccgccttgag caattccaga
     1261 ttttcgtcgg tgtattcagg ggcgagcggt ttacccgcca ccactttcgg accaccgcca
     1321 tgcatcttca atgcgcggac ggttgcaacc atcaccacta catgcgggat caagccagaa
     1381 taacggcatt tgatattgaa gaacttttcc ataccaatat cggcaccgaa accagattca
     1441 gtgaccacga agccatcttt gccgactaac ttgagcgcaa ttttgtcagc gatgatggaa
     1501 gattgtccgt gagcgatatt agcgaaaggt ccggcgtgaa ccgtggcagg agtcccttcg
     1561 agggtttgca ttagattggg cttgatggcg tccttcatca aaacagtgac agcgccagct
     1621 acaccaagat cttccacagt cacagcgtcg ccgcgtttgc tggtggcaac caccatcttg
     1681 ccgaagcgtt cgcgcatgtc ttcaattcca gtggtcaatg caaggacagc catgatttct
     1741 gaggcaacgg tgatgtcgta gccagacata tgtgcatgac ctacctcgtc tttaccacga
     1801 cctacttcca cttcacgcaa catgcggtca ttgatatcta ctacgcggcg ccaagtaatg
     1861 gtacgttcgt cgatgtcgag gcgagcgaag cgggagcgtt cttcaggggt gagatcatct
     1921 ggatttgttt tcttaatgcc aagtttttcc aaacggcgta acatagtggg tgagaatttg
     1981 cgctgtccct ttttgttggg cgggcagagc gcgttgaaga gcttctcatc atcttgttct
     2041 ttttcatgca tgacacgcgc atcaagagcg gcggcaacca agttatgtga ggcggtaatg
     2101 gcatgaatat cgccggtgag atggagattg aaatcttcca tcggaatgat ctgggaataa
     2161 ccaccgcccg ctgcgccgcc tttgatacca aaagtgggtc ccattgaagg ttggcggatg
     2221 gctgtaataa cctttttacc gaggtgcgca ccgagcgcct gagaaagacc gaccgtggtt
     2281 gtggttttgc cttcaccaag cggagttggc gtaatggcag ttacatcgat gtactttcca
     2341 ttgggtttgt tcttaaggcg ttcaaggatt tcaagcttaa tcttggcttt gtagggtccg
     2401 aacaattcaa gttcgtcatc gcgaatacca agctctttgg caatttgggt gatcggctta
     2461 agctttccag cttgtgcaat atcaatatcc gaaggcacag gtcgaaggag ctttatctta
     2521 gtcggttgga atgtgttgct gggcttggga gcagctttct tctttgctac gacctttttt
     2581 gcaactttct taggcgcagc ttttgcaccc ttcttaacgg actttccttt ggttgccata
     2641 gagtctcctt gaaaattata tataatttat tctatcccca attatcagac aaatttaaaa
     2701 aattgcaaga gcaaaccgtc atttttttgt catttcatgc agttagcaac gttcccacat
     2761 tttcaccttg caaggcacgt gtcaagaaac ctggctcttc tgccgagaaa atttgtacag
     2821 tcaaacccgg cttcttttgg atgagggaca gcatttcttc cactttggct ttcatcccac
     2881 cagtgacatc ggtgctcacg gaacccttga tgccagaccg catcgcttca taatccgata
     2941 gttgaatctg ctttacgagc tttgtcctcg ctgggaaatc tgcccacact ccggcttcaa
     3001 tcccagccag cagaattcga gtggtaggga attggtccac gagaaaggtg aaaacatctt
     3061 ctgtagaaag aatggtgccg cctagggttt catcaaaagc gacatctccc tgcaccacgg
     3121 gcagcaaatc cacgcccagg gcgcggcgga tggtctctac attgtgatgg gtcactttcc
     3181 gtttgtctga aaccatcgaa gcagatggcg ggaaaggtag cgcaggtatc cctgcctcaa
     3241 agagagaggt gacaacataa cgatttaact cggcggcttg aagccgaact tcagtgaagc
     3301 ccagccattg ttccttcgtt tggacgccat cgcgagtgcc atgcttcttt gcggcaacgt
     3361 gcccgaacga gccagaacca tgcccgagaa tcagtttcaa gcccggggtt gagtccagag
     3421 ccgttttgat ttccttggca aggtttgcca gtttatccag ccttggggtg tagggagtgt
     3481 ctttatcggt aatgagcgat ccgcccagtt tgaggagaac gagttcgttg gtcatagtgc
     3541 ttgttttaac acagattttt gaaattgtcg cgcactttgc tttactgggt ataattttgg
     3601 catggatatt cgtactggtg tcttcgtcct cgccctgttt gcggctgtgg gggcgtttct
     3661 ttctttccgt ggagcgatcc gcaatatgca ggcggctcgc aagatttctt tttatagctt
     3721 acgtaagcgt tacaacaata ccgcttggcg tttggttttt cttgccttta ttttgatcgg
     3781 tgttgctttt tggttcccgt ctggtggggc gcaagtgatg tatcaggtct ttccgccttc
     3841 tgtcacccca tcgttgacgc cgacgattac actgacgccg accattacgt taacgccgac
     3901 tatcacctta acgccaacac taacgcccac gccattggtc tctgatacac caacattgac
     3961 tccaactccg ttcttgccgg ctgcgattga agcgcttttt gctggacctg tcacgccgaa
     4021 ccctgattcg atatttaccg ccattcaatt ctctacagag tttgacggcg taaatgcagt
     4081 ggacccaaag actgttttta cacttcctat tgacacgatt tatggcggtt tcgattacaa
     4141 caacacgcaa cccggcgtgc agtggaccgc gctctggttt cgtaatggcg aattggtttg
     4201 ttatgagacc gaaccttggc gtgaagaatg gggtacgggc ggtattggtg ggtatacctc
     4261 ttgttcttca cctttggatg gttggcaggc agggcaatat gaagtgcaga ttttcatggg
     4321 atacgattgg aaagtcgttg ggcgttttgt ggtgatggat agcttgactc cttctgttac
     4381 ggtaactggt acgccagacc tgacgcaaat aggaacccca accgtctcgc cgacagcaac
     4441 tccataaatc tatatattac aggactacac atgacagaaa aagcatttca agaatactat
     4501 cctgaacatt tatctcattg ttatggttgt ggcacgatga atgaattggg acatcagatc
     4561 aaaagctatt gggacgggga agagtcagtg tgtcactttc agcctaaacc ttaccatacc
     4621 gccattcccg gttatgtgta tggtgggttg ctcgcttctc tcattgactg tcacggtact
     4681 ggaaccgccg ctgcggctgg ataccgtgct gagaatcgct ctatggatac agaacctgcg
     4741 cttcgttatc tcaccgcttc tttgcacgtg gactatctca agccaacgcc gatgggacca
     4801 gtgcttgaaa tccgcgcgag ggtgaaagaa gttaaggggc gtaaggtagt ggtcgaagag
     4861 tggattcagg ttaatggagt ggtcaccgtc aaaggagaag tggtggcggt acaagttcca
     4921 gaagcattgg tgaatgaatt gcttgaaaaa ggtgcaaatt aaattcaaag agtagagaat
     4981 aaaaaatgaa aaaagtatgg attggtattt tacttggggt ggtcttggct ggctgttcaa
     5041 cgaacgtccc gcctgaaatg cgtgcaagga tgatggatac agaaaatgtc ccttccgagt
     5101 ggatcatttt caatgaatcc aaaggtgaag attggggcgg tcagttgtat acagttgcat
     5161 tcgcgtatgg gaatgaatca gaatcgccgg cattggaaca gcaattgatc gtgtattcag
     5221 atgagtctgc tgcgataact gggtatgatg aatttgatgc gtatatcttc gtggaagagt
     5281 ggacggtcaa ccctgaaatc acgttttccc cgagcacagc gacagacaaa tttgcggcaa
     5341 agtgtacaga ccgtgaaatt gaccatgtga tgaccaaaat ctgttttgtg ttacagcaac
     5401 atgcgaacta tgtctctgct ttgggcgttc acatgggcgg acccattacc tttgacgtgt
     5461 tgaactcggt cttaaaatct attgatacaa aattgaacca ataaaaagaa ggtctcgcta
     5521 aaacgagacc ttctttttta gaaccactag gcggctacag tttcagtcgc attcttcatt
     5581 acagttgcta aggtgacgta ggtctccgtc caagcttcca tgacttcggg ggtaaagtct
     5641 tcgccgagcc ctttttccag cgtccacagc agggcgtccc ccacggtatc gtagtgagag
     5701 tcttttacgc catagccaac atggcggcga ccaagatttt ccaccgcagg cactaactga
     5761 tcgaggttat ccaaaccttt tacagccatg ccgagcattt gcaataactt catcttttgt
     5821 tctttcatat ctgcattctt gaacaaaggc ttaagttcag ggtcgatctc aaaaaggcga
     5881 ccgtagaata gttcagcagc agtttctgcg atcggtacga ctttttccca ggtttcttgc
     5941 acaagttttt tctgttctaa tgtgatgctc atttctaaaa tactcctcta aagttaattg
     6001 attaatacga gcgcgatttt ataagcatcg cttggaattg accatgtgag tttggtacta
     6061 aaactcagat aggacattac tcctataaaa aagagctgca ttttatgcag ctctttttta
     6121 ttttatccaa aatattcttt cattggcttg aaataaaaat caagccagcc tgttttatag
     6181 ctctctattt gatcttcagg catatgtgta tggcgtagcg tgagctttgt tttgccatta
     6241 gtttcttcca gcaagatttc tacacgagag tcttcggcat cgtcagggaa ttctccggtg
     6301 cgccaaagtt gcacgatgcg tttgttgggt tccagatcaa gaaaggtgcc ccaaatatac
     6361 ccatcccatg cggtgaaatc acccttggcg tgcccgttga cctttgccgg gcttccggtc
     6421 atcaaggtat gtccctgtgt ggacaaccag gcgttgtata tggctgcggc gttggcattg
     6481 aaggtttcag aaagaacaaa ctcatttttc atcctgcctc cggcgatcaa acgaatctac
     6541 gctatctatc taaatcgccc attccttttt tcgcgtgtac atctggtttg caatttcacc
     6601 tgctaaacgt gcattgttca ataacaatgc cagattggtc ttaagagatt taccctttgt
     6661 caattcgcta atacgaccca gcaagaaagg agtcagtgcc tgcccgtgga tgcctttctc
     6721 tacagcttca gcagatgcct gagcgatcat cggctccatt tcagactttg agatggcatc
     6781 tgcttcggga accg
//
LOCUS       DAOQFT010000048         6774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5513_length_6774_cov_6.257181, whole genome shotgun sequence.
ACCESSION   DAOQFT010000048 DAOQFT010000000
VERSION     DAOQFT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5513_length_6774_cov_6.257181"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLR93_02140"
     CDS             <1..439
                     /locus_tag="PLR93_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH25786.1"
                     /translation="PVYRYLYSRLGDAHSAEDVTSQTFITAYEALPKYREQGQFTAWL
                     FRIARSKMYDYLRKNKREVGLEAAGELLEREDALGTLIRAEELSKVRFLIKQLNEEEQ
                     DLIRLRYVADLTFVEIADLLGKREDAVKKTLYRLLARLKGQME"
     gene            442..1962
                     /locus_tag="PLR93_02145"
     CDS             442..1962
                     /locus_tag="PLR93_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HNH25787.1"
                     /translation="MNDQNPSPEFEEKVREAMHMPEADPKFAKRLRNELVGRPVKMKT
                     KIFRPAWAIAFVLALVVLAINVPRLAAALGRVFGYIPEVGLVETTNGLRMLAEPISST
                     RDGVTITITNVFAYPDRIELQYEVSGVAPEDDGWQSSDASSNPTAFCGGVNPGSTYNK
                     AGDARLRLPDGTVLERDYTGKYPKNVFAMQPVYDGALPAGVTSLTFVLDCIPQARLGV
                     VPEHWELALDLVEVPAGTVVGEPVIEVTQPVVTEEVPASDSTETVSAPKVTITLERIV
                     PTDTNTVVYFHLNMENADPSLVSIMPRSVYVIDSLGQQFRMVGSFVWQPFEHKVGSAF
                     EFVSEGKPATGPLTVVVDEMTAYYMPMYVDPPQATPEEMTFTFDAGENPQYGHKWDLN
                     KTFTIAGYDFEIVSAQASNYDDVAKIHDYIDGSQGYEYGYQFTVAADPSLELLVEMDI
                     RSDEYQCWLSDVNKVETQPLLYTQLCKDGYPKRQVSVTIREMSIALDDDVQAEWIP"
     gene            1995..2378
                     /locus_tag="PLR93_02150"
     CDS             1995..2378
                     /locus_tag="PLR93_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25788.1"
                     /translation="MLKALQENMMNKSLIPFFLPFIIVACGSKGDEVPDVLLTPPAIV
                     VNASRDNCPSIEAEVGMTIAWENVDTVPLPIKIEFLDDNGKVTDTGLSVIDPETVFST
                     QFQEAGVYHVYCTENADTYSTITVK"
     gene            complement(2464..3036)
                     /locus_tag="PLR93_02155"
     CDS             complement(2464..3036)
                     /locus_tag="PLR93_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNH25789.1"
                     /translation="MTTLAPTIYNRTFPRVSEVVRDITLILLGSWLIALFAQIEIPLP
                     FSPVPITGQTFAVLLVGALLGSKRGAAAMVAYIAQGAAGLPFFAGGASGFGILTGATA
                     GYLVGFIVAAYVVGLLAERGMERSFKTSIVPFLVGTIIIYAFGVTWLSSVVGGFGKAI
                     ELGLLPFVVGDAIKLVAAAVALPAAWKFVR"
     gene            complement(3149..4396)
                     /locus_tag="PLR93_02160"
     CDS             complement(3149..4396)
                     /locus_tag="PLR93_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNH25790.1"
                     /translation="MILDETHPLEEVMVWGEPGIEALLGQLLPQSRSLFRSYYEVPKA
                     RGEFACMSSLIESEGIKVTRAKDAVADLLKTREIPELPGSIQDLKRILHQKADSTFEH
                     YRLAKQSDLIRDGITNISPIDVYNAVKRDIDVVLEEDIAQYGEQGALRLNYLLSLSKP
                     WPMANIFYGRDQSQALTDRIILSSLRWNIRKPEVRIFKDALHHMGYGTVLADIREGTI
                     EGGDVAMFKGYCFIGVGARTNINAVKEVYRQIGGSLKEHRIELIAVVNNQHEEDSNFF
                     ISPTSEHMQVMHLDMFWIPLTQDLVMAYTTELDHREVVRVWQEKGRILTERLGTFREF
                     MSIIGVEIFEVDEAEQKNFATNLLNFGNKKVIAALSSNPRVNAELERRGFTVKYAELT
                     KLVDGYGAVHCLTAPLKRRIPNS"
     gene            complement(4462..6264)
                     /gene="argS"
                     /locus_tag="PLR93_02165"
     CDS             complement(4462..6264)
                     /gene="argS"
                     /locus_tag="PLR93_02165"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNH25791.1"
                     /translation="MFQKEQQLIEEKIKDYCAANDIALAELKWQPIPFSGEWGFATSF
                     FQTAANEAKLGKGTKAPVPQKAQEIAEQVKGQLGSVAGISHIEAVKGYLNVYFKTSEY
                     AHRVVEEVLKNGAEFGRGEAKPETVMVEYAQPNTHHSFHIGHARNTILGEVLARLVDF
                     AGYKTIRASYPGDLGLGVITVMWIYDKLYKGQEPAGIHERGQWLLKLYVEANQLLEKK
                     ENETPEQTAQREAYEAERREMYRKWDAGDEYVRELWRVTREWSLEELREVLHMLDVKM
                     DVWFYESEVDEPSKAIVEELIAKNIADDERPNGGAVIVKIDEKLGLTKEKYRTNVILR
                     RDGTTLYLTKDLALAKVKFEQYHVDRSIYVVDSRQALHLQQAFAILKLWGFKQSEKCY
                     HLGYGFVSLPEGAMSSRKGRVVLFKEVFDEAVKRVLSVNAEKSVEIAAEEREKVAAQI
                     GLGALVYSMLSVDNNKDIVFNIEEALSFDGRTGPYIQNAHVRATSILKKAGKVSEADF
                     NYDLTKQEIELIEQMSQFPQKVQQAAEEYRPLVMAMYAYDLANAFHSFYHAVPVLQSE
                     DEKVKNARLRLVAAAKQAIANALHLLDIQSPDVM"
     gene            complement(6339..6611)
                     /gene="rpsT"
                     /locus_tag="PLR93_02170"
     CDS             complement(6339..6611)
                     /gene="rpsT"
                     /locus_tag="PLR93_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNH25792.1"
                     /translation="MANIQSQIKRNRQNEKRRLRNRNVRGAARTADNAARKVLTSDTP
                     ESKQAVMQAISALDKAAEKGVLHKNTVARRKSRLMKALAALTKKSA"
BASE COUNT         1685 a         1645 c         1621 g         1823 t
ORIGIN      
        1 acctgtctat cgctacctgt acagccgcct cggtgatgcc cattctgcag aagatgttac
       61 atcgcagaca ttcatcactg catatgaagc gttgcccaag tatcgagagc aaggtcaatt
      121 cacggcgtgg ttatttcgaa tcgctcgcag caagatgtac gactacttgc gcaagaacaa
      181 gcgcgaggtc gggttggaag cggcaggcga gttgcttgag cgtgaagacg cactcggaac
      241 gttaatccgc gcagaagagt taagcaaagt ccgttttctc attaagcaat tgaacgaaga
      301 agaacaggat ttgattcgct tgcgctatgt ggctgaccta accttcgttg agatcgcaga
      361 tttgcttggc aagcgcgaag acgccgtcaa aaagactctt taccgtttgt tggctcgttt
      421 gaagggtcag atggagtgaa tatgaacgat caaaatccct ctcctgaatt tgaagagaag
      481 gttcgcgaag caatgcatat gcctgaagcg gaccctaaat ttgcaaagcg attacgcaat
      541 gaactggtag ggagaccagt aaaaatgaaa acaaaaattt ttagacccgc ttgggcgata
      601 gcattcgtgc ttgctctagt agttcttgca ataaatgttc cgagattggc tgctgcatta
      661 ggtcgtgtgt ttggatatat tcccgaagtg gggttggtgg aaacaacgaa tggtttacgt
      721 atgttagcag aaccgatttc atcaactcgt gatggcgtca cgattacgat cacaaatgta
      781 tttgcttatc ctgatcgtat tgaattgcaa tatgaagtga gtggcgttgc tcctgaagat
      841 gacggctggc agtcatctga tgcatcgtcg aacccgactg cattttgcgg cggcgtgaat
      901 cctggcagta catataacaa agcgggcgat gcgcgtttgc gtctgcccga tggcacggtg
      961 cttgaacgtg attacaccgg caaatatccg aagaatgttt ttgcgatgca gcctgtttat
     1021 gatggtgcgc ttccggcggg tgtcacttct ctcaccttcg tgctcgactg tattccgcaa
     1081 gcccgtctcg gcgttgtgcc tgaacattgg gaactcgcgc ttgatcttgt cgaagtcccg
     1141 gctggaacag tcgtgggcga gcccgtgatc gaagtgactc aacctgttgt gacagaagaa
     1201 gtgcccgcct ctgactcaac cgaaactgtc tctgctccca aagtgaccat aaccctcgaa
     1261 cggatcgtcc ctactgatac caacacagtc gtttacttcc acctaaacat ggagaatgcc
     1321 gatccttctt tggtctccat catgccgcgc agtgtttacg tcatcgactc gctcggtcaa
     1381 caattccgta tggttggttc attcgtttgg cagccttttg agcataaagt gggcagtgcc
     1441 tttgaatttg tcagcgaagg caaacctgct accggtccct tgaccgtggt ggtcgatgaa
     1501 atgaccgcct attacatgcc catgtatgtg gaccctccac aggcaacccc tgaagaaatg
     1561 acatttactt ttgatgcggg cgagaatccg caatacggtc ataaatggga tttgaacaag
     1621 actttcacca ttgccgggta cgactttgag atcgtctctg cccaagcttc gaactatgat
     1681 gatgtcgcga aaatccatga ttatatagac ggttcgcaag gatatgagta tggctaccaa
     1741 tttaccgttg ctgccgaccc atcgttggaa cttttggttg aaatggacat tcgaagtgac
     1801 gaataccaat gctggctgtc agatgtcaat aaagtggaaa ctcaaccttt gctctatacc
     1861 caactttgta aggatgggta cccgaaaaga caggtttctg tcaccatccg cgaaatgtcc
     1921 attgcgttgg atgatgatgt tcaagccgaa tggataccat aggaacttta taccattcag
     1981 gagaattcct ttcgatgtta aaagctttgc aggagaatat gatgaataaa agtttaatcc
     2041 ccttttttct gcctttcatt attgtcgcct gcggttcaaa aggtgatgaa gtgccagatg
     2101 tgctcctcac tccgcctgcc attgttgtga acgcttcgcg ggacaactgt ccttccatag
     2161 aagcagaggt aggaatgacc atagcttggg aaaatgtcga tacggtgcct ctgccgataa
     2221 agatcgaatt cttggatgac aatggaaagg ttactgacac gggattgtct gtgatcgacc
     2281 ctgaaaccgt gttttcaaca caatttcaag aagccggggt ttatcatgtc tactgtactg
     2341 aaaatgctga cacctattca acgattacgg tgaaataaaa aaactcccca tacgcctgca
     2401 agaatatatg caggtgtatg gggagtaaat aattgtctta ttctaggcgt gtgatgtttt
     2461 tagttatcgg acgaatttcc aagctgcagg caatgctaca gcagcagcga ctaacttgat
     2521 agcatcacca accacaaatg gtagcaatcc taattcgata gctttaccaa aaccgccaac
     2581 gacggagctc aaccaagtga cgccaaaggc atagatgatg atcgtgccga ccaaaaatgg
     2641 aacaatggag gtcttgaagc tacgttccat gcctcgttct gcaagcagac ctacaacata
     2701 cgctgccaca ataaagccaa cgaggtaacc agctgttgcg ccggtgagaa tgccaaatcc
     2761 gctggcgccg cctgcgaaga aaggcaaccc tgccgcgcct tgagcgatat aagcgaccat
     2821 ggcagctgca ccacgcttgg agccaagcaa ggcaccgacc agcaatacgg caaacgtctg
     2881 accagtaatt gggacaggag agaaggggag tggaatttcg atttgagcaa aaagcgcgat
     2941 caaccacgaa ccgagtaaga tgagggtgat atcgcgcacg acctcagaaa cacgaggaaa
     3001 ggtgcggttg tagatggttg gtgcaagtgt ggtcatataa aatctatctc ctaagtagaa
     3061 tgtcaatagt tataacacgc aaacccttgt gaagatttgg atagggatta atgtcattct
     3121 gtccccactg taggatgaca tgccattttt atgaatttgg tatacggcgt tttagcggtg
     3181 cggtgagaca gtggactgcg ccgtatccat cgaccagttt ggtcaactcg gcgtatttga
     3241 ccgtgaagcc acgtctttcc aactctgcat tcacgcgcgg gttggaagat aatgccgcga
     3301 tcactttttt gttgccaaag ttgagtaaat tcgtggcgaa gttcttttgt tcggcttcgt
     3361 ccacttcgaa gatttctaca ccgattatgg acataaattc acggaaagtt cctaatcgtt
     3421 ccgttaagat acgccctttt tcctgccata cacgcacaac ttcgcgatga tccagctcag
     3481 tcgtgtatgc catgaccaaa tcctgtgtaa gtggaatcca gaacatgtcc aaatgcatga
     3541 cttgcatgtg ttcacttgtc gggctgatga agaaattgct gtcttcttca tgctgattgt
     3601 tgacgacggc gatcagttcg atgcgatgct ctttcaacga gccgccgatc tggcgataca
     3661 cctctttgac cgcgtttata ttcgtccgtg cgccgacacc gataaagcaa tagcctttga
     3721 acatagcgac gtcgccgcct tcaattgtcc cttcgcgaat gtctgctaga acagttccat
     3781 agcccatatg atgtagcgcg tctttgaaaa tgcgcacctc aggttttcgg atattccagc
     3841 gtagagaaga aagaatgata cggtctgtga gggcttgcga ttggtcacgt ccgtaaaaga
     3901 tatttgccat gggccagggt ttggagaggc tcagtaaata atttaggcgt agagcacctt
     3961 gttccccata ttgcgcaatg tcttcttcga gtacaacatc aatatcccgt tttactgcgt
     4021 tgtatacatc tataggggag atgttggtga ttccgtctcg gatcaagtcg ctttgtttag
     4081 cgaggcgata gtgctcaaag gtagaatccg ctttttgatg gagaatccgt ttcaggtctt
     4141 gaatagagcc gggtaattcg ggaatttcac gcgtcttcaa taaatctgct actgcatctt
     4201 ttgctcgcgt aaccttaatc ccttcgcttt cgatcaagga agacatacaa gcaaattcac
     4261 cgcgcgcttt gggtacttca taataactgc ggaacaggct gcgcgattgc ggtaacagtt
     4321 gaccaagcaa agcctcaatg ccgggctcgc cccaaaccat gacttcttct aaaggatgtg
     4381 tttcatcaag aatcatttat accttcactt ataaaaacag actaaatcct caaggattta
     4441 gtctgttttt tgtactgatt tttacatcac atctggcgac tgaatgtcta atagatgaag
     4501 cgcattggca atggcttgtt tcgccgctgc caccaatcgc agacgggcat tcttaacctt
     4561 ctcatcttct gattgcaaga ccggcaccgc atgatagaac gaatggaagg cgttcgccag
     4621 atcgtaggca tacatcgcca tcacaagcgg acgatactcc tctgccgctt gctgcacctt
     4681 ctgcgggaac tgcgacattt gttcaatcaa ctcgatctct tgcttggtca ggtcataatt
     4741 gaagtccgct tcagaaactt tgccggcttt cttcaaaatg gaggttgctc gcacatgcgc
     4801 attctgaata tacggacccg tgcgcccatc aaaagagagc gcttcttcga tgttgaagac
     4861 aatatcttta ttgttatcca ccgaaagcat ggagtagacc aaagcgccca aaccaatttg
     4921 agccgcaact ttttcacgtt cttcagcagc gatctccacg ctcttttcgg cattgaccga
     4981 gagcacgcgc ttcacggctt catcaaagac ttccttgaaa agcaccaccc gtcccttgcg
     5041 ggaagacatg gcaccctcgg gcaggctcac gaaaccatag ccgaggtgat agcatttttc
     5101 cgattgcttg aaaccccata acttgagaat ggcaaatgcc tgttgcaaat gcaatgcctg
     5161 gcgtgaatct acaacataga tcgagcgatc tacatgatat tgttcgaact tcactttggc
     5221 taatgccaaa tctttggtca ggtaaagggt tgtaccgtcg cggcgcaaaa tgacgttcgt
     5281 gcgatacttt tctttggtca acccaagttt ctcgtcgatt ttcacgatta ctgcgccgcc
     5341 gttcggacgt tcatcgtctg cgatgttttt ggcgatcaac tcttcaacga ttgctttcga
     5401 gggttcgtcc acttcagact cgtagaacca cacatccatc tttacatcaa gcatgtgcag
     5461 cacttcgcgc agttcttcca aactccactc gcgggtcaca cgccataact cgcgcacata
     5521 ttcatcgcct gcatcccact tgcgatacat ctcgcggcgt tcggcttcgt aggcttcgcg
     5581 ctgagcggtc tgctcaggcg tttcgttctc tttcttttct aacaactgat tggcttccac
     5641 gtataacttc aagagccact gtccgcgctc gtgtatgcct gcgggttctt gtcccttata
     5701 caatttgtcg tagatccaca tcacagtgat gacgccaagt cccaagtcac cagggtatga
     5761 agcacggatc gttttgtatc ctgcaaaatc aaccaacctt gccaacactt cacccaagat
     5821 cgtattgcgc gcatgcccaa tatggaatga gtgatgcgtg ttcggctgtg catattcaac
     5881 catcaccgtt tccggctttg cttcgccacg cccaaattca gcaccgtttt tgagcacttc
     5941 ttccacaaca cgatgagcat attcagatgt cttaaagtag acgttgagat agcccttcac
     6001 cgcttcaatg tgactgatac ccgccacgct tcctaattga cctttgacct gctcggcaat
     6061 ttcctgagct ttttgcggca ctggggcttt tgtccctttg ccgagttttg cttcgttagc
     6121 agcggtttgg aagaacgaag tagcgaaacc ccattcaccc gagaagggga tgggttgcca
     6181 ctttaattct gccaatgcaa tgtcattggc ggcgcaatag tctttaattt tttcttcgat
     6241 gagttgttgt tctttttgaa acatgattgc tcagattata tcaactacag ataacaaaaa
     6301 cgggagtctt gcgactcccg ttgcgattcg agtgctaatt acgcgctctt tttggtcaac
     6361 gctgcaaggg ctttcatcaa gcggctcttg cgacgcgcga cggtgttctt gtgcagcaca
     6421 cctttttcag cagccttatc caaagcgctg atggcttgca tcacagcctg cttggattcg
     6481 ggggtgtcgc tggtgagaac tttgcgcgca gcgttatcgg cagtacgggc ggcgccacgg
     6541 acattgcggt tacgcaggcg acgcttctcg ttctgacggt ttcgtttgat ctgagattga
     6601 atgttagcca aggtcttcct ccaaaaaatt cttgccttac tttacgacgg cgacctattt
     6661 tacatggcac aggcatattt gtccagtaaa atgataagaa aaccgcgggc aatttgcccg
     6721 cggtttttgt ttatggggtg gctgttgccg gcgccgcgtt gatgggggtg gagg
//
LOCUS       DAOQFT010000049         6709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5591_length_6709_cov_8.244214, whole genome shotgun sequence.
ACCESSION   DAOQFT010000049 DAOQFT010000000
VERSION     DAOQFT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5591_length_6709_cov_8.244214"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2610
                     /locus_tag="PLR93_02175"
     CDS             <1..2610
                     /locus_tag="PLR93_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH25793.1"
                     /translation="ERKQAEEALRVSEENYRGLVESSDNAIAVFDRDGNVLYANQSSQ
                     QLWNAPELVGKTLYDLFPKDFADHHLISMKKVIDEQIIDTNELEVVIHGNKMWFHVTM
                     SPLKNSDGSVDRLLLNALNLTERKKAEEAQRRSEILFFKVFRASSIGIHLFRLSDGRS
                     YNVNDAFLEIIGYPREELIGRTAVELNLFEDIETRNRWMQALREGRSVRNQDTRIRRK
                     SGEIRDCLASLDLIDVSGEVMVLVIATDITERKRAEVQLRQSEERYRTTLDSMLEGIQ
                     IIDSDWRYTYINEAAAKQGQSSPEQLLGRTMMEAYPGIEHTELFSILRDCMTHRTVKR
                     FENKFIFPDGSSGCFELNVQPAPEGIFILSMDITERKKAEEQLRGSEERFRLMAENIE
                     EGFWITNPNSDEEIYLSPAVERIFGRSMNDLMARSSSFIESILPEDVPLVLTNMERQK
                     LGTATNMEYRIKRPDGRIRWVWDRAFPILNPNGTVRLVTGITTDITERKATDAKIQTQ
                     IKRLSALNSIDRAISSSMRMDVALDVLLIEVVAQLNVDAASILLLNETDQTLDFAAGK
                     GFNTSIEHASVPLGFELAGKVGTERKVIHVPNLSELKKGVKRKELIQEEHFLEYFGVP
                     LIVKGQLKGVLEIFNREALNPDPEWLTYLETLGGQAAIAIESAQLFEGLQRSNQELFM
                     AYDATIAGWSHAMDLRDKETEGHTQRVTELTVRLAERMGISQQQRIHIRRGALLHDIG
                     KLGVPDHILLKPDKLTDEEWHIMRMHPVYARDMLTPINYLRPSLDIPYCHHEKWDGTG
                     YPRGLKGEDIPLAARLFAIIDVWDALRSDRPYRAGWSAEKTRDYIQSESGKHFDPKVV
                     EYFLALLEELSKS"
     gene            2810..>4650
                     /locus_tag="PLR93_02180"
     CDS             2810..>4650
                     /locus_tag="PLR93_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH25794.1"
                     /translation="MTAISKAMPKNKKGETGQKRQIKTSRANSPSAHHHIMNEVNLDI
                     NNKSKTAIQRLKAENARLQADLETLMRERRTMAETSLRQSQELDLLGRVRAAIATKLE
                     LPALLHSVVESIVEICGYAYVSIYLLEGETLILQHQAGYQKVIEKINIHEGISGRVAR
                     TGQPILIEDAKGEPEFLQASKKIRSEITVPLMDNGQVTGTLSIESTSKLTDADLRIMM
                     ALGAEINVAIERSRLYEDLQKRNRTLSALQEATLTIIGKLGLPDAVQTILAQTAQLLS
                     TSHGYVYLVQPNGTLQMATGLGVYPKYIGLSLESGEGLAGKVWETATPLTVNDYIHWD
                     GRSSKFNEHSFHAVMGAPLLHNFKVIGVIGLSHLDAGRTFSKSDEDLLIQLAQLASIA
                     IDNAALYTQINEELVERKQADERLRKSDQRLQVFFNQSLDGFFFCDFEEPIPWKHAFN
                     KDETLEYIISTKPFTDVNDAMLKQYGVDRDTFLRLTTKDVFAHDLHQGRNLRRQLFDK
                     GYLHLETNERTLEGNQIWFEGDYVCLYDSLGRITGFFGIQRDITERKQAESELKRHVQ
                     NTLTMYELSQNIHTSLDLDLVYQETHQAVQKLMPCDAFLIALLDKKN"
     assembly_gap    4651..4750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4751..6493
                     /locus_tag="PLR93_02185"
     CDS             <4751..6493
                     /locus_tag="PLR93_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH25795.1"
                     /translation="MFGYDSRDEMLSIDIKKDLYFSPEERNSLFLDVGQEKVDEFRMK
                     RKDGSEIWVEDHGRYIHDENGNVIYHEGILRDITERVYASEAARKAEEKYRSIFENAV
                     EGIYQSSPDGKFHTVNPAFAAMLGYTSPQEMMLSIKDIANQVYSDAYRRQDFTKQIEE
                     KGEVNGFEYQMRRKDGRLIWVSENARLVRDTKGEILYYEGILEDITARKQNEQRIDNQ
                     LKQLNALHAIDNAINSNFDLRTTLDVILREVTAQLRADATSVLLFNKETHTLDYTAGR
                     GFISKATQHTKLSIGEGYAGRVILDRKMIHIPNLTQTENYRLAEALNLAGENITAYVG
                     SPLIAKGQVVGVLEIFQRAPLAPDPDWFDFLSMISDQAAIAIDNAQLFENLQRSNFDL
                     TLAYDATIEGWSRALDLRDRETEGHTQRVTNLTVKLARQMGVPDADILHIRRGALLHD
                     IGKMGIPDKILHKPDKLTAEEWAVMRQHTNYAYQMLTPITYLKPALDIPRCHHEKWDG
                     TGYPDGLKGEQIPIAARIFAVVDVWDALTSDRSYRDAWTKEKAIEFIRSESGKHFDPK
                     VAETFLSFILKETP"
BASE COUNT         1929 a         1654 c         1497 g         1529 t
ORIGIN      
        1 gaacgcaaac aagctgaaga agccttgcgc gtgagtgaag aaaattatcg cgggttggta
       61 gagtcttctg ataacgcgat tgccgtgttt gatcgagacg gcaatgtgct ttatgcaaac
      121 caatccagcc agcaattatg gaacgccccc gaactggtcg gcaagaccct gtacgacctc
      181 ttcccgaaag actttgcaga ccaccatctc atttccatga aaaaggtcat cgatgaacag
      241 atcatcgaca ccaatgaact ggaagtcgtg atccatggaa acaagatgtg gttccatgtc
      301 accatgagtc cgctaaaaaa ttcagacggc tctgtggata ggctgttgtt aaacgcgctc
      361 aacctcaccg agcgcaaaaa agctgaagaa gcgcaacggc ggtctgaaat cttgttcttt
      421 aaagtcttcc gtgccagttc catcggcatc catctcttcc gtctttccga tgggcgctcc
      481 tataatgtca atgatgcgtt tcttgagatc atcggttacc ctcgcgaaga attgatcggg
      541 cggactgccg tagagttaaa tctgttcgaa gatattgaga cgcgaaatag gtggatgcaa
      601 gccctacgag aaggcaggag cgtccgcaat caagatacca gaatcaggcg caaatctggc
      661 gagattcgag actgcctcgc atcactggac ttgatcgatg tcagcggcga ggtcatggtg
      721 ctggtgattg ccaccgacat taccgaacgt aaacgcgccg aagtccaatt aagacaaagc
      781 gaagaacgct accgcaccac tctagatagt atgttggaag ggattcagat catcgactcc
      841 gattggcgct acacctacat caatgaagct gccgccaagc aagggcaaag ctcgcctgaa
      901 caactcctgg ggcgcaccat gatggaagcg taccccggca ttgagcacac ggaacttttt
      961 tccattctgc gtgactgcat gacccaccgc acggtcaaac gctttgaaaa caaattcatc
     1021 ttccccgatg gttccagcgg ctgttttgaa ctcaacgtgc aacctgcccc ggaaggcatt
     1081 ttcatccttt cgatggatat cacagagcgc aaaaaagctg aagagcaatt gcgcgggtca
     1141 gaagaacgct tccgcttgat ggctgaaaac attgaagaag gtttttggat cacaaatccc
     1201 aacagcgatg aagagattta tctcagccca gcagtggaac ggatctttgg tcgcagtatg
     1261 aatgacctta tggcgagaag ttcctcgttt atagaaagca ttctgccaga agatgtgccg
     1321 ctggttctaa cgaatatgga aagacaaaaa ctgggtaccg ccaccaatat ggaataccgc
     1381 atcaaacgtc cagatggtcg catccgctgg gtctgggatc gtgccttccc gatcctaaac
     1441 ccgaatggaa cggtgcggct tgtgacgggt attaccaccg acatcacaga acgcaaggca
     1501 accgacgcca aaattcaaac gcagatcaag cgtctcagcg ccctcaactc catagaccgg
     1561 gcaatcagtt ccagcatgcg catggatgta gctctggatg ttctccttat tgaagtcgtc
     1621 gcccagctta atgtggatgc tgcttccatc cttcttttga acgaaaccga tcaaacctta
     1681 gacttcgcag caggcaaagg gtttaatact tccatcgaac atgcaagtgt gccactcggg
     1741 tttgaactgg ctggtaaagt tggcactgaa cgaaaagtaa ttcatgtgcc caatctgtct
     1801 gaattaaaaa aaggggtcaa aaggaaagag cttatccaag aagagcattt cctggaatac
     1861 tttggtgtgc ccttgatcgt aaaaggacaa ctcaaaggcg tgcttgagat attcaaccgc
     1921 gaagccttga acccagaccc tgaatggtta acctatcttg aaacactggg cgggcaagct
     1981 gccattgcta ttgaaagcgc acaactattt gaagggttgc aacgctccaa ccaggaactt
     2041 tttatggcat acgatgccac catcgcaggt tggtctcatg ccatggattt gcgtgacaaa
     2101 gaaaccgaag gtcatacgca gcgcgtcaca gaactcaccg tccgcttggc agaacgcatg
     2161 ggaatcagtc aacagcaacg gatccatata agacgcggag ccctcctcca tgacattggc
     2221 aagctgggtg tcccagatca tattctgctc aagccagata aactcaccga tgaagaatgg
     2281 cacatcatgc gcatgcaccc cgtttacgcg cgcgatatgc tcacccctat caactatctg
     2341 cgaccgtcgc ttgatatccc ttattgccat cacgaaaaat gggatggcac aggctatcca
     2401 cgcggattaa aaggtgagga tatcccgctg gcagccaggc tctttgcgat tatagatgtg
     2461 tgggatgcgc tccgctcgga tcgtccctac cgcgcgggct ggtctgccga aaagactcgc
     2521 gactacatcc agtctgaatc tggcaaacat tttgacccaa aagtcgttga atacttcctg
     2581 gcacttctgg aagaactttc aaaaagctga caactcaaca aaaaagacaa cccttgtggt
     2641 atcacaaggg ttgtcttttt ctattaggag tagatgttac ccatttttga gatttataac
     2701 attgcagcat cagcaaagag agcaatcata ttgtaatttc atctaacata gtgggtatgc
     2761 tatactgatt ttaagcccca gagacttgtg gagtagagga aataatggaa tgaccgccat
     2821 ctctaaagca atgcccaaga ataaaaaagg ggaaactggg cagaagagac aaataaaaac
     2881 gagcagagca aattcaccat ctgctcatca tcacatcatg aacgaagtaa acctcgatat
     2941 taacaacaaa tccaaaacag caattcagcg cctaaaagct gagaatgccc gtctgcaagc
     3001 agaccttgaa acgttaatgc gtgaacgccg cacgatggca gaaacgtctt tgagacaatc
     3061 tcaagaatta gatttattgg ggagagttcg tgccgccatt gctacaaaat tggaactgcc
     3121 tgcactttta cacagtgtcg tcgaatcgat tgtagaaata tgtgggtacg cttatgtcag
     3181 catttatcta ttagaaggtg aaactttaat ccttcagcat caagcagggt atcaaaaggt
     3241 catcgaaaag atcaacatcc acgaaggcat tagcggacgt gtcgctcgaa ctggtcaacc
     3301 cattcttata gaagatgcaa aaggcgagcc ggaattcctt caagcctcaa aaaaaatacg
     3361 ctctgagatc accgtgccat taatggacaa cggtcaagtg accggcacgt tgagcataga
     3421 aagtacgtcc aaactcaccg atgccgatct acggatcatg atggcattag gcgctgaaat
     3481 caatgtagcg attgagcgtt cacggttata tgaagacctg caaaaacgca accgcacttt
     3541 atctgcctta caagaagcaa cgcttactat catcggcaag ttagggctgc ccgatgcggt
     3601 tcaaaccatt ttggctcaaa ccgctcaatt gttgagcacc tcacatggct atgtttatct
     3661 ggttcagccg aatggcacac ttcagatggc aaccgggctg ggcgtgtacc caaaatacat
     3721 tggcttgagc ttggaatcag gcgaagggtt ggctggcaaa gtatgggaga cagcaactcc
     3781 actcactgta aatgattata ttcattggga tggtcgctct tccaaattta atgaacattc
     3841 cttccatgcc gtcatgggag ctccactgct gcataacttc aaagtgatcg gggtcattgg
     3901 tctttcgcat ttagatgccg ggcggacctt ctcaaaaagc gatgaagatt tattaattca
     3961 actggctcag ttggcatcga tcgccatcga taatgcggct ttatacactc agatcaacga
     4021 agaactggtg gaacgaaagc aagcggatga acgcctgcga aaaagcgacc aacgtctaca
     4081 ggtgtttttc aaccaatcgt tggatggctt cttcttctgc gattttgaag agccaattcc
     4141 gtggaagcat gcgttcaaca aagacgaaac cctcgaatac attatttcga caaagccatt
     4201 taccgatgtc aacgatgcca tgttgaaaca atatggcgtt gaccgagata ccttcctgcg
     4261 cttaacgacc aaagatgtct ttgctcacga cctacatcaa ggacgcaatc tacgcaggca
     4321 attgttcgac aaagggtatc tgcatctgga aaccaatgaa cggacattgg aaggcaacca
     4381 aatttggttc gaaggcgatt acgtatgttt atacgactct ctcgggcgta tcactggttt
     4441 ctttggcatt caaagagata tcacggagcg caaacaggct gaaagtgaat tgaagcgaca
     4501 cgttcaaaac acgctcacca tgtacgagct cagtcaaaac atccacacaa gcctagactt
     4561 ggatttggtc tatcaagaaa cgcatcaggc tgttcaaaag ctcatgcctt gtgatgcctt
     4621 cttaatcgct ttattggata aaaagaatca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn atgttcggct acgactcacg cgatgaaatg ctctccatcg atatcaaaaa
     4801 agacctctac ttcagccctg aagaacgcaa cagcctcttc cttgatgttg ggcaagaaaa
     4861 agtagatgag ttccgtatga agcgcaaaga tgggtctgag atctgggtgg aagaccacgg
     4921 acgctatatt catgatgaaa atgggaatgt tatctatcat gaaggcatct tgcgcgatat
     4981 tacagaacgc gtctatgctt cagaagccgc tcgcaaagcg gaagaaaaat atcgcagcat
     5041 ctttgaaaat gccgtagaag gtatttacca aagttcaccc gatgggaaat tccacaccgt
     5101 aaaccccgca tttgctgcca tgctaggata cacctcgccg caggaaatga tgctatccat
     5161 taaagacatt gccaatcagg tctatagcga tgcctatcgt cgccaagatt ttacgaaaca
     5221 aatcgaagaa aaaggcgagg taaatggctt cgaatatcaa atgcgccgca aagatggccg
     5281 gctgatctgg gtctcagaaa atgcccggct cgtgcgcgat acaaagggtg agattctata
     5341 ttacgaaggc attctcgaag atattaccgc tcgcaaacaa aatgaacagc gcattgacaa
     5401 tcaattaaaa caattgaacg cattgcacgc cattgacaat gccatcaatt ccaacttcga
     5461 tctgcgcacc accctcgacg tcatcttacg agaagtgaca gcacagttgc gagcagacgc
     5521 cacttctgtg ttgttattca ataaagaaac ccacacctta gattacacag ccgggcgcgg
     5581 tttcatctct aaagccaccc aacataccaa gctcagtatc ggtgaaggat atgccgggcg
     5641 cgtcattcta gaccggaaga tgatccacat tccaaacttg acccaaactg aaaattaccg
     5701 tctcgccgaa gcgttaaacc tcgctggaga aaatatcacc gcctatgtgg gttctccgct
     5761 catcgctaag ggacaagttg tcggcgtgct ggagatcttt caacgtgcgc ctctcgcccc
     5821 cgacccagat tggttcgact tcctgagcat gatctcagac caagccgcta tcgccataga
     5881 caacgctcag ctctttgaaa atctacaacg ctcaaacttc gacctcacac ttgcatacga
     5941 tgccaccatt gaaggctggt ctcgcgcctt agacctacgc gatcgcgaaa ccgaaggaca
     6001 cacccaacgc gtcaccaacc tgaccgtgaa actggcacgt caaatgggcg ttcccgatgc
     6061 cgatattttg cacattcgcc gtggtgcctt gctccacgac attggcaaaa tgggcatacc
     6121 cgataaaatc ttgcacaagc cagataaact tacagccgaa gaatgggcag ttatgcgtca
     6181 acacactaac tatgcctatc aaatgttgac acccatcacc tatctaaaac cagctctgga
     6241 tattccacgt tgccaccacg aaaaatggga cggcaccggc taccccgacg gactaaaagg
     6301 cgagcagata cccattgcag cacgaatctt cgccgtagta gatgtttggg atgcgctgac
     6361 cagtgacagg tcctatcgtg atgcgtggac aaaagaaaaa gctatcgaat tcatccgatc
     6421 agagtctggg aagcattttg atcccaaagt cgcagaaacc ttcctgtctt tcattttgaa
     6481 agaaacacct taaaccaaaa gacggcggat gaaatcatcc accgtctttt tatctacgct
     6541 gctttattta gcgcgccaac accttcacca tgtggtaata ctccatgttg accttgcgct
     6601 tattactaat gacatcgccc aaggggataa agtcaatgcc tttacccttc agtcccgtca
     6661 tcacgccatg tgtaccttcg atcaaagctt ctacagcttt cacacccat
//
LOCUS       DAOQFT010000050         6694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5611_length_6694_cov_6.202741, whole genome shotgun sequence.
ACCESSION   DAOQFT010000050 DAOQFT010000000
VERSION     DAOQFT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5611_length_6694_cov_6.202741"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="PLR93_02190"
     CDS             complement(<1..1820)
                     /locus_tag="PLR93_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH25796.1"
                     /translation="MKTIVRSLALLSIASLLLASCTLFGSSTEPAANALSEVQATQSL
                     TDLVTIELTTQYDTAAVYNTVGQTVRFKYLVKMVKNDLPDGTPANVTITGITATCPAL
                     NTINNLNDRFDPGEVLECTGDYLITQADLDKGSIANVVTANAYSVNSNTVTTTVPTVS
                     PKALTLTKTASTTTYGAANQQITFTYTVKNSGTVQLGPAQFTIADTGISNNQPFNCGN
                     ADATIAPGATLNCNATYTVTAADMNATTISHSATVSGGGVTSQPAALSLTKGSAPAAA
                     SGTTVQHTVREGEWLWQIARCYSADPNQTVSANPQVDPKNLKIGTTVTVPNVGTVGNR
                     AVHAPPELCVQLHTVQSGDTWTSIADKYGADPGLTQLSNSNNMIVGKQVKVPLYTKGL
                     NIPLSNAVATVSPTTTGMSLTVTPNTTIYTQVDQIITFNYVIKNNGSTTLGPTQFMIN
                     DVLMSPTAFKCGLENSTIAPGATLTCSNAYKTTQNDLAVANLQFSTTASGTNVPTTTS
                     VATTLTKGTAQLSLTVTPSATTYNTAGQTITFTYTIKNTGSGDLGPTQFFINDPLMNP
                     ATLNCGAANTTLAPNATVQCTNNYTITDADLGAVNIQFSS"
     gene            complement(1954..2439)
                     /locus_tag="PLR93_02195"
     CDS             complement(1954..2439)
                     /locus_tag="PLR93_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25797.1"
                     /translation="MANGQVKKIATPKAAGYFIVYKVQPTDKSLKQIAREQLADEKLW
                     EKIVLDRETANPKGFFWDEIPANSFDPRWTLLLPPSEFAAFTISVNTPIHRAPNGEFL
                     AGANITEKYFYARKTVVVSGQKVWADVTKTNPARFGNKPYWILVKDGTSKTNPPISGP
                     S"
     gene            complement(2505..3347)
                     /locus_tag="PLR93_02200"
     CDS             complement(2505..3347)
                     /locus_tag="PLR93_02200"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH25798.1"
                     /translation="MKSIVVTGVSTGIGRSISELLIQNGFHVFGSVRKQADAERLNAD
                     LGTGFTPLTFDVTDEVAVQAAANQVRDALNGETLFGLVNNAGIAVAAPILHIPIEDFR
                     KQMEINVTGQLIVTQAFVPLLRAKDHQSKAGRVVNISSVSGKNGSPFLGAYAASKHAL
                     EGLSESMRRELMLYGIDLVIVAPGPIATPIWDKAEELKAEQYQHTDFYEPGKKFQKYA
                     IANGRKGLPPEAVSKIVFKALTLSRPRVRYAVDPNPFSNWVMSILPKRTIDKILAKGL
                     GFNK"
     gene            complement(3428..3796)
                     /locus_tag="PLR93_02205"
     CDS             complement(3428..3796)
                     /locus_tag="PLR93_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25799.1"
                     /translation="MKFYYWHYGIIFCSLATAILHIMCYPYFGWLDSIVLNGFGSIAL
                     LLAYFLPIPFFRRNRQTTYWIIVSYTLITIVLWIFLGDKAFNFENTSSIGYFAKIAEM
                     FLLIFMGFDFPRKPAPARAS"
     gene            3892..4401
                     /locus_tag="PLR93_02210"
     CDS             3892..4401
                     /locus_tag="PLR93_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25800.1"
                     /translation="MAKNISLNDIHIRTDLQPGDTGGIMSMHGRLYYKEYGYTTVFES
                     YVAKGLGEFLEKYDPKRNRIWVCEHAGNMVGTLVLMDRGEAAQLRYFLLEPQYRGFGL
                     GTRLMDLFMEFFQDCGYASSYLWTTEQQLTAAKLYKRYGYQLTEEKPSTAFGVSLTEQ
                     RYDLIFSRI"
     gene            complement(4405..4830)
                     /locus_tag="PLR93_02215"
     CDS             complement(4405..4830)
                     /locus_tag="PLR93_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH25801.1"
                     /translation="MKQQLHLITLGVKDFKRSYEFYTKTLGWKPSGASTEDVAFFQTS
                     GVVFAIYPREKSAEDALTSPDGSGFSGFTLAYNTSSESEVDEIIADLKAKGVKIVKEP
                     QKAFWGGYSSYFADPDDYRWEVAFNPFFQFDEKGNLNLE"
     gene            complement(4870..5361)
                     /locus_tag="PLR93_02220"
     CDS             complement(4870..5361)
                     /locus_tag="PLR93_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNH25802.1"
                     /translation="MANHTFVKFCPRCGTPVDYQEKFGAIRPVCPQCHFIHFQDPKVA
                     AAVLIQEGSRVLLVRRVNEPHRGLWTLPAGFVNGGEDPAEAAARECLEETGLTVQVTR
                     VHDVIAGKEHERGADFIIIYAAKVISGEMNAADDADAVEWFDQDHLPPLAFAATKKVL
                     LSI"
     gene            complement(5364..6086)
                     /locus_tag="PLR93_02225"
     CDS             complement(5364..6086)
                     /locus_tag="PLR93_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25803.1"
                     /translation="MKNFYDVETLSAYLDGQLDASNAARLEARLKTDAELASALRDLR
                     AARGILKKLPARKAPRNFTLTRQMVGLKPPLPRTYPLLRFATAFAAILFLFSFTANTL
                     VPMFNFGAAAPAYGLGSGGGCDACGGGGPESAPMAAAATEAAATEAPATELFAAQEAA
                     PASTLTTDAADTAPIAETETVRIMETPSAKEPAPETANQAEVQQEPPLSPVNWTWIFL
                     GIAILGGLVLWIMQFTARNKWR"
     gene            complement(6083..>6694)
                     /locus_tag="PLR93_02230"
     CDS             complement(6083..>6694)
                     /locus_tag="PLR93_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH25804.1"
                     /translation="SMDETALIASAQQGDLNAFNTLILHYQDAVFNTAVRILGDDDMA
                     QDAAQDAFISAFQSIKSFRGGSFKAWILRTVTNACLDELRRQKRRPTTPLEPETHDGE
                     EMDSPKWLADSSMTPAQKAEADELEHAIQHCLDALPTEFRTAVVLADVQGMDYTEVAT
                     AIRAPLGTIKSRIARARLRLRECLRSFEELLPSSFRLEEENAQ"
BASE COUNT         1639 a         1481 c         1726 g         1848 t
ORIGIN      
        1 ctgctgctga actggatgtt tacagcgccc aagtctgcat cggtgatggt gtaattgttt
       61 gtacactgca cggttgcatt gggtgccagt gtggtattgg ctgcaccgca attcaatgtg
      121 gctggattca tcaatgggtc attaatgaag aattgcgtag gacctagatc tccgctgccg
      181 gtgttcttga tcgtgtaggt gaaggtgatg gtttgcccag cagtgttgta tgtggtggca
      241 ctcggtgtta ctgtaaggct gagttgagca gttccttttg tgagggtggt ggcaactgaa
      301 gttgtagttg gtacgttcgt tccagatgct gttgtactga attgaaggtt tgccactgcc
      361 aggtcgtttt gggtggtttt ataggcgttg gaacaggtaa gggtcgcgcc gggggcgatg
      421 gtcgaatttt ccaacccaca cttgaatgcc gtagggctca tcagcacatc gttgatcatg
      481 aattgggtcg gtccaagagt cgtagacccg ttattcttga tgacatagtt aaaggtgatg
      541 atctggtcta cctgtgtgta aatagtcgta ttcggggtga ccgttagcga cattcctgtg
      601 gttgttgggg aaactgtggc aaccgcattg gaaagtggaa tatttaaacc cttggtataa
      661 agtggaactt taacctgctt gccgacgatc atattgttgc tgttagagag ttgtgtgagc
      721 ccggggtctg caccatattt atcggcgatg gacgtccacg tatcgccaga ttgaacagta
      781 tggagctgaa cacaaagctc tggcggtgca tggacggcgc gattcccgac agtgccaaca
      841 ttgggaacag tgactgtagt gccgatcttc aagtttttcg ggtccacttg cgggttcgcg
      901 ctaacggtct gattgggatc tgccgaataa cagcgggcga tttgccatag ccattctccc
      961 tcgcggacgg tgtgttgcac ggttgttccc gaagcagcag cgggcgcgga acctttggtt
     1021 agtgaaagag ccgcgggttg tgaggtcaca ccgccacctg aaacggttgc agagtgagaa
     1081 atagtcgtcg cgttcatatc tgcagctgta acggtgtagg tggcgttgca gttcaaggtc
     1141 gcaccggggg cgatggttgc atctgcattg ccgcagttga acggctgatt attgctaata
     1201 cccgtatcgg caatggtgaa ttgtgccgga cctaattgaa cagttccgct attcttaacg
     1261 gtataggtaa atgtaatttg ctgatttgct gcaccataag ttgtggtgct tgccgttttc
     1321 gtaagcgtga gtgctttggg cgaaacggtt ggcactgtgg ttgtcactgt gtttgaattc
     1381 accgaatacg cattggcagt gactacgttt gcgattgaac ctttatcgag gtctgcttgg
     1441 gtaataagat aatctccggt gcattcaagc acttcgccgg ggtcgaagcg gtcattgaga
     1501 ttattgatag tgttgagcgc cgggcaagtg gctgtaatgc ctgtgatcgt aacatttgcc
     1561 ggagtgccat caggcaggtc atttttgacc attttgacga gatacttaaa gcgaacagtt
     1621 tgcccaacgg tattgtagac tgctgctgta tcgtattgag ttgtaagctc aatcgtgact
     1681 aaatctgtaa ggctttgagt tgcctgaact tcagacaatg catttgcggc gggttctgtt
     1741 gagctgccaa agagcgtgca agatgccagc agtaaactgg caatgctcaa cagggcaagc
     1801 gagcggacga tggttttcat gaccacttcc tttcgttttg taaaattgta cgaccacttt
     1861 tattatatcc tctatacatc tggattaaga atcatcccgc agggttctta agtctactct
     1921 gcgggatgat aatgcgagga ttaaagcaac aaattaactg ggaccggata taggtggatt
     1981 tgttttggat gtaccgtcct taacaaggat ccagtagggc ttgttcccga atcgtgcggg
     2041 atttgtcttg gtcacatctg cccaaacttt ttgcccactg acaacgactg tcttgcgcgc
     2101 ataaaaatat ttctcagtga tattcgcgcc agccagaaat tctccattag gcgctctatg
     2161 aataggcgtg ttgacggaga tggtaaacgc tgcaaattca gaaggcggca gcaacaaagt
     2221 ccaacgtggg tcgaaagagt tggcgggaat ctcgtcccag aaaaagcctt tgggattcgc
     2281 ggtttcacgg tctagaacga tcttctccca gagcttttca tcggcaagct gttcacgagc
     2341 aatttgtttc aagcttttgt ctgtaggctg aactttatat acaataaaat acccggctgc
     2401 ttttggagtt gctattttct taacttgtcc gtttgccatt tttctctcct ttgtttttga
     2461 accacaacta tagaataccc gaatagaagt ggatgtcaat agctttattt gttgaagcca
     2521 agtccctttg cgaggatttt atcaattgta cgtttaggta agattgacat gacccaattt
     2581 gaaaatggat tggggtctac tgcatagcgc acgcgcggac gggaaagagt caacgctttg
     2641 aatacgatct tgctcacagc ttcaggtgga agacctttgc gcccattggc gatggcgtat
     2701 ttttgaaact tcttgcccgg ctcataaaaa tcagtatgct gatattgctc ggcttttagt
     2761 tcttcggctt tatcccagat tggcgtagca atcggacccg gcgctacaat gaccaagtca
     2821 atgccgtaga gcatcagttc gcggcgcatg gattcggata agccttccaa tgcatgttta
     2881 gaggcggcat atgctcctaa aaatggactg ccattttttc ctgaaacaga cgagatatta
     2941 acaacccgcc cggctttgct ttgatgatct tttgctcgta acaaaggtac aaatgcttgg
     3001 gtgacaatca attgcccggt gacattgatc tccatttgct tgcgaaaatc ttcgatgggg
     3061 atgtgcaaaa tcggtgcggc aaccgcaatc cctgcgttgt tcaccaagcc aaacaaagtc
     3121 tcgccattca aagcgtcacg tacttgattg gcagcggctt gaacggcgac ttcatccgta
     3181 acatcaaagg tcaatggggt gaagccagtt ccgaggtccg cattgaggcg ctctgcatcc
     3241 gcctgcttgc gtacactgcc gaagacatga aatccgttct ggattaataa ctcactgatc
     3301 gaccgtccga ttcctgtcga tacccctgta acaacaatac ttttcatcaa gagatccttt
     3361 tgaacgtgga tatactgata ttttacctat aaaataaaag agacagcttt gtgctgtctc
     3421 ttttatttta tgatgctctg gcgggtgctg gctttcttgg aaagtcgaag cccatgaaga
     3481 ttagcaagaa catttcggct attttggcaa aatagccgat ggaggaagtg ttctcaaagt
     3541 tgaatgcttt atcgccgagg aagatccaaa gcacgatggt aatcagggta tagctgacaa
     3601 tgatccaata ggtggtttga cgattacgcc tgaaaaatgg gatgggtaga aaatatgcca
     3661 gcaataaagc gattgagcca aaaccattta gcacgatcga gtctagccag ccgaagtagg
     3721 ggtaacacat aatgtgcagg atggcagtag caagactgca gaagatgata ccgtaatgcc
     3781 agtaataaaa tttcattgat aatgctccac gtataaagtt ttttagtaga cgtattttat
     3841 gggagattta ttacaaaggc aagcaaatat tcaatgatat gattgcgcct catggcgaaa
     3901 aatatttcac tcaatgacat ccacatccga accgatttgc agcctggcga cacaggtgga
     3961 attatgtcga tgcatggtcg gttgtactac aaagaatatg gatacaccac tgtttttgaa
     4021 agctatgtag caaaagggct gggcgagttc ttagaaaaat atgaccctaa acgaaaccgc
     4081 atttgggttt gtgaacatgc tggaaatatg gttggaactt tagtcttaat ggatcgtggc
     4141 gaagcagcac agttgcgcta tttcttgctg gaaccacagt atcgcggatt tggcttaggt
     4201 acgcgcctaa tggacctttt tatggagttt tttcaagatt gtgggtatgc ctcgtcctat
     4261 ctgtggacga cagaacagca gttgacggct gccaaacttt ataaacgcta tggctatcaa
     4321 ctcacagagg aaaagccttc aacggctttc ggtgtttcac ttaccgaaca acggtatgac
     4381 cttatctttt ctcgtatata aaacttattc caaattcaga ttgccttttt cgtcaaattg
     4441 aaagaatggg ttaaatgcta cttcccagcg atagtcatct gggtctgcaa agtaagaact
     4501 atacccaccc caaaatgctt tttgcggttc ttttacgatc tttacccctt tggctttcag
     4561 gtctgcaata atctcgtcca cttcagactc actgctggtg ttgtatgcca aagtgaaacc
     4621 tgagaagccg cttccatcgg gagaagtcaa tgcatcttct gccgatttct cgcgggggta
     4681 aattgcaaac accacaccac tggtttgaaa gaaagctaca tcttctgtgc ttgcaccaga
     4741 aggtttccac ccaagtgtct ttgtataaaa ctcatatgat cttttaaagt cttttacgcc
     4801 caacgtgatc aaatgtaatt gttgtttcat gtgttgctcc ttttgaacaa actacatctc
     4861 caccaaacgt tagatagaaa gcaatacttt tttcgttgcg gcaaatgcca gaggcggtaa
     4921 atggtcttgg tcaaaccatt ccacggcatc ggcatcatct gccgcattca tttctccact
     4981 tatcactttt gctgcataga tgatgataaa gtctgcgccg cgctcgtgtt ccttcccggc
     5041 aataacatca tgtacgcgcg tcacttgcac ggtgagcccg gtctcttcca aacattctct
     5101 ggcagccgcc tctgccgggt cttcaccgcc gttaacgaaa ccagccggta aggtccacag
     5161 tccgcgatgt ggctcgttca cacggcgcac aagcaaaaca cggctgccct cttgtatgag
     5221 gacagccgct gctactttcg ggtcttgaaa atggatgaag tgacattgcg ggcaaaccgg
     5281 tcgtatggcg ccaaactttt cttgatagtc tacaggcgtt ccgcaacgtg ggcaaaactt
     5341 tacaaacgta tggtttgcca tccctaacgc cacttgttac gtgctgtaaa ttgcatgatc
     5401 cacaaaacca atccgccaag gattgcgatg ccaaggaaaa tccaagtcca gttgacggga
     5461 gagagaggcg gctcttgctg gacctctgcc tgattcgcag tttcaggagc tggttccttc
     5521 gctgaaggcg tttccataat gcgtacggtt tcggtttcag cgataggtgc cgtatcagct
     5581 gcatcggtcg tgagagtgga ggctggtgca gcttcttggg ctgcaaataa ctcagttgct
     5641 ggggcttctg tagcggctgc ttctgtggct gccgctgcca tcggagcaga ttcgggtcca
     5701 ccgccgccac aagcatcaca gccgccgcca gaacctaatc catatgcggg ggctgctgca
     5761 ccaaagttga acattgggac gagtgtgttt gctgtgaacg aaaataggaa gagaatggct
     5821 gcaaatgcag ttgcaaacct aagcaatgga taggtgcgcg gcagaggtgg tttgagcccc
     5881 accatttggc gggtaagggt aaagttgcga ggagctttgc gtgctggcag ctttttgaga
     5941 atgccacgag cagcgcgaag gtctcttaaa gcagaggcaa gctcggcgtc ggttttgagg
     6001 cgggcttcta gtcgcgccgc attcgaagca tcgagttgtc catctaaata tgcagagagg
     6061 gtttcaacat cgtaaaaatt tttcattgag cgttctcctc ttccagacga aatgaggagg
     6121 gcaaaagttc ctcgaaggaa cgtaaacact cacgcaacct taatcgtgcg cgtgcaattc
     6181 tgcttttgat ggtgcccaat ggcgcacgga tcgcggtagc aacttcggtg taatccatgc
     6241 cttgcacatc ggcaagcaca actgctgtgc ggaattcagt gggcaacgca tcgaggcaat
     6301 gttgaatagc atgttcaagt tcatcggctt ctgctttttg cgccggtgtc atgctcgaat
     6361 ctgctagcca tttgggagag tccatttctt caccatcgtg ggtttcaggt tcaagcggag
     6421 tcgtggggcg gcgtttctgg cggcgcaatt catcgagaca ggcattcgtc acagtgcgca
     6481 ggatccaagc tttgaacgag ccgccgcgaa atgatttgat gctttggaat gctgagatga
     6541 aagcatcttg tgcagcatct tgcgccatat catcatcccc aaggatgcgc acagctgtgt
     6601 tgaagacagc atcctgataa tgcaggatga gcgtattgaa cgcgttcagg tcgccttgtt
     6661 gggcagaggc aattaatgca gtttcatcca taga
//
LOCUS       DAOQFT010000051         6673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5630_length_6673_cov_6.443336, whole genome shotgun sequence.
ACCESSION   DAOQFT010000051 DAOQFT010000000
VERSION     DAOQFT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5630_length_6673_cov_6.443336"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1572)
                     /locus_tag="PLR93_02235"
     CDS             complement(13..1572)
                     /locus_tag="PLR93_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH25805.1"
                     /translation="MANNLIGKTISNRYVIEEMLGQGGMSAVYKGNDPNLKRVVAIKV
                     IHSHLSGNPDFVQRFEEEAAAVAQLRHPGIIQVYDFNRDDDLYYMVLEFVPGETIQDH
                     LKRLNDSGRKLSPTKAAEYMAGICDAVDYAHQRGMIHRDIKPANLMLTITGQVILMDF
                     GIAKIVGGTRHTATGAVVGTAMYMSPEQIKGEQPDRRTDIYSLGVTLFEMVSGRPPFE
                     AESAMTLMMMHINDPVPDPKKLNPDVPDALVAVIQKALAKDPNNRYQSAAQMAAALRN
                     LLSAGSSSPLATMMEDATIMNSSRGTMIEKPYAGTYVESKPIAPAPGTMNKNSAPSSM
                     YATPAPAASAPKRKNLLLLPIIAVVAIGLICLVGGVAFAAKQLFGGVVETEPPVVLAT
                     NTQPAIVAPIETEVSTETPIPATPTPSSPYVVITAIRVEGSLYAVDYDVHNFTDGLHV
                     HIFFNTVPPEQAGSPGSGPWKLTWGAYGNPPFTQYSIAARPANATQMCALVANSNHSV
                     QPNSGNCVDLP"
     gene            complement(1662..2579)
                     /locus_tag="PLR93_02240"
     CDS             complement(1662..2579)
                     /locus_tag="PLR93_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25806.1"
                     /translation="MLISLACGTSGSTASRESTVEAISAQVRATGTAAAAQSSQGTQP
                     VVETNSDSLTATAVAFAPILESLPKYGVDPSEGKIGWIHPPVSLDASGYHQFEYINYY
                     IGTLAKDFVISSDITWNTVGSISGCGFVMRSDGNTNALDQYVAVITRVASGHFVFSTM
                     SDGEVVTGRDIYARYKDKSFRWENDTTNRLTIVGRGNHFWIYTNDTLIGEVDPSAPPP
                     QPNLPPEPDKPANPSDPQAMALYEQQKIEREAIIVQMQADYAARKRAYNSAETVFERG
                     FMALVALSESGRSTNCKFDNTWLFLIDSP"
     gene            complement(2625..5822)
                     /locus_tag="PLR93_02245"
     CDS             complement(2625..5822)
                     /locus_tag="PLR93_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNH25807.1"
                     /translation="MNSFKLSLINDQSSAPSKEFELTKSEIIIGREDSVDITIPASAV
                     SRRHARLMLIGENYVLEDLGSSNGTYVNGEKLDKQHTLKSGDKIRLGQIISLVYTAPV
                     SEEDSNLTVVSIKDANKTAKHSGPAKPAHVMQTVLGEAPAIPQASAGPRQLIVSIAGG
                     STQTYPLTGSSISIGRLDTNDIVIPSQIVSSRHARLEQVKGGGYKLVVMPEAKNPVQF
                     EGRPIDGSHVLHHNDLLRIGSMDPGVMVTITYNAPSEASQQMERDIVFGEKTLIQIGR
                     DPSNDVVIPSPNVSRFHTQIEKVGQRYRVEDLRSSNGTYVNGERIEGSVWLKPNDSIR
                     IGQYRFVMGKDQLARYDDGNGLRVDVVGLNKWVRKDLNILQNIYASFSPREFIVVVGQ
                     SGGGKSTFVDAVAGYRPATPPSKVYVNDIDIYTHFDAIRNEIGFVPQKDIIHMELTIY
                     QALDYAAQLRMPADTSPEERHKRIMEVLEDLDLTHRKDVQISGLSGGQQKRVSIGVEL
                     LTKPGLFFLDEPTSGLDPGTETALMQLMRRLADQGRTIILITHATKNVMLADKVIFLA
                     RGGYLAWFGPPDEALAYFDQYRSERARRAGKIEFDEIYAILDDPSKGKAEDWANRYRS
                     SNAHTEYVLKPLAGKLSPQSVATVPQPERLPQKAANTRQVSSLRQLMILSARNIKILT
                     RDRFALGLMLATAPVVSLLDVILSFVMGREIFDFYKGDMSQVMITLFLLTIYGIMVGG
                     ISQMREIVKEQDIYRRERLVNLKIFPYVLSKVWVAALLALYQAVAYTVVHYISFRMPG
                     GVMEFIQIYITMTLATFAGMMLGLFASALAPNSSSAPLLVIILMLPQIVLAGALIPVP
                     SFVSAPFSTRWAFESLIAISGSGSDVAADVCWALPPDVRNAMSLEEKLANNCKCMGVN
                     LLKKESCNHPGIGKFYIPLVDQPLPIPPAPLPPEPVRPVEPQDQSDNIAMADFFAKLK
                     IYEAEVELYKAKVDKYQEEQVAYQTVISQLKVQVATAEAVVRTFVNGSGFAYVDKDDP
                     LAYHTKVILTWTVQSFIIVLLFVAILYLQKRKDVI"
     gene            6173..>6673
                     /locus_tag="PLR93_02250"
     CDS             6173..>6673
                     /locus_tag="PLR93_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH25808.1"
                     /translation="MMQSSSFSRKLSEENNSLVRTLRRRADLHPERLAFRFLQDDESE
                     NITINFGELDRRARAIGAWLESFNAKGERALILYPPGLDYIAAFMGCLYAGVIAVPAY
                     PPRLNRPVPRIQSIVDDSHATFALTTTSIHSNIEKRFEHAPDLKALHWLNTEQVPAGL
                     EADWRDP"
BASE COUNT         1720 a         1526 c         1537 g         1890 t
ORIGIN      
        1 cgtgagttac ttttacggca aatctacgca gtttccagag tttggctgaa cagagtgatt
       61 cgaattggca actaatgcac acatttgagt tgcgttcgcc ggtcgagcgg caatgctgta
      121 ttgagtgaag ggtgggttgc catatgcccc ccaggtcaac ttccagggac ctgatccagg
      181 cgagcctgct tgttccggtg gaacagtgtt gaaaaatata tgcacgtgca atccatcagt
      241 gaagttgtgt acatcataat ccacagcgta aagacttcct tcgactctga ttgcggtgat
      301 gactacgtag gggctcgaag gggttggtgt tgccgggatt ggagtttcag ttgagacctc
      361 tgtttcaata ggagctacga ttgcgggttg agtattggtc gcgagtacaa ccggcggctc
      421 tgtttctaca acacctccaa agagctgctt ggctgcaaaa gcaactcctc caacaagaca
      481 aattaatcca atagccacta cggcaatgat cggcagaagt aacaaatttt ttcgctttgg
      541 agcagaagcc gccggcgccg gggttgcgta catcgaactt ggcgctgaat ttttgttcat
      601 tgttccaggt gcaggagcga taggtttact ttccacatat gtgcctgcat atggtttctc
      661 tatcattgtt ccacgcgatg aattcataat ggtggcatct tccatcatcg tggcgagggg
      721 tgaagatgaa ccagcggaaa gcaaatttct tagtgcggct gccatttgag cagcgctctg
      781 ataacgattg ttcggatcct tcgcaagcgc tttttggatg actgcgacca aagcgtctgg
      841 cacatcagga ttgagctttt taggatccgg caccggatcg tttatatgca tcatcatcaa
      901 tgtcatggct gattcggctt caaaaggcgg acgtccgctg accatttcaa acaaggttac
      961 gccgagagag taaatatctg tgcgccggtc gggttgttct cctttgatct gttcaggcga
     1021 catatacatt gcggttccga caaccgcccc ggttgctgtg tgacgtgtac cgccaacgat
     1081 cttggctatg ccgaaatcca tcagaataac ttgacctgtt atggtaagca ttaagttagc
     1141 tggtttaata tcgcggtgga tcattccacg ttggtgcgca taatcgactg catcgcaaat
     1201 gcctgccatg tattcagcgg ctttggtggg ggagagtttt ctaccgctat cgttgagtct
     1261 tttcaagtga tcctgaattg tttccccggg cacaaattcc aaaaccatat aataaagatc
     1321 atcatcgcgg ttgaagtcat agacttgaat aataccggga tggcgtaatt gtgctactgc
     1381 tgcagcttcc tcttcaaaac gctggacgaa gtcagggttg cccgataaat gggagtgaat
     1441 gaccttgata gccacaacac gctttaagtt ggggtcattg cctttgtaaa ctgcagacat
     1501 cccaccttgc cccaacattt cttcgataac atatcgattg ctaatggtct ttccaattaa
     1561 gttattagcc attgctactc ctagaaaata aaaacattga cactcttaaa taatattcgt
     1621 aatactgaaa taacgctcag ctttataccc tactttaatc cttatggtga atctatgaga
     1681 aaaagccatg tgttatcgaa cttacaattg gtacttcgcc ctgattcgct taacgctacc
     1741 aatgccatga agcctcgctc gaagaccgtt tctgctgagt tgtatgctct tttacgtgcg
     1801 gcataatcag cttgcatttg tacgatgatc gcctcacgct caattttctg ctgttcgtac
     1861 aatgccatcg cttgcggatc ggatggattg gcaggcttat ccggttcagg tggaagattg
     1921 ggctggggtg gtggggcgct tgggtcaact tcaccaatca aagtgtcgtt ggtgtaaatc
     1981 caaaaatgat ttccacgccc tacgattgtc agacggttgg ttgtgtcatt ttcccatctg
     2041 aaacttttgt ctttatatct tgcataaata tccctacctg ttacaacttc gccatctgac
     2101 atggtggaaa aaacaaaatg tccacttgct acacgggtaa tgactgccac atattgatcc
     2161 aatgcatttg tattgccgtc agacctcatg acaaacccgc agcctgaaat actgccgaca
     2221 gtattccaag taatgtcaga agatattaca aagtctttcg ccagtgtacc aatataataa
     2281 tttatgtatt caaattggtg ataaccagat gcgtccagcg atactggcgg atggatccag
     2341 ccgatcttac cctctgaagg atcaacgcca tatttgggca ggctctccag aattggagca
     2401 aatgccactg ctgttgctgt caatgaatct gaattcgtct ccacaactgg ctgggtgccc
     2461 tgtgaacttt gtgctgctgc cgctgtacca gttgcacgga cttgggcact aattgcctca
     2521 acagtacttt ctcttgaggc tgtgctgccg ctggttccac aagccaacga gattagcata
     2581 aagatcaacg ggaatattat gagtctattt tttcgcatcg ggcactaaat cacatcttta
     2641 cgtttctgca gatataaaat agcaacaaac aataagacaa taatgaaact ttgcacggtc
     2701 caggtcaata taaccttggt gtgatatgcc aacgggtcat ctttatcaac atatgcgaaa
     2761 cctgatccat tgacaaaggt ccggacaacg gcttcggcag ttgcaacttg cacctttagc
     2821 tggctgatta ctgtctgata ggcgacctgt tcttcttgat acttgtcaac ctttgcttta
     2881 taaagctcaa cttcagcttc gtagattttg agtttggcaa agaaatctgc catggcaata
     2941 ttgtctgatt gatcttgtgg ctcaacaggg cgaacaggtt ccggcggtaa tggcgcgggc
     3001 gggattggca atggttggtc cacaagcggg atgtagaact tgccgatgcc aggatgatta
     3061 caagattcct ttttaagtag attgacaccc atgcatttgc aattattggc aagcttttct
     3121 tccaaagaca tcgcattgcg aacatcaggt ggtagcgccc aacaaacatc tgccgcaaca
     3181 tcagacccag accctgagat ggcgatcaga gattcaaatg cccaacgtgt tgagaagggg
     3241 gcgctcacaa aacttggaac cggaattaat gcaccagcca atacgatctg cggcagcatt
     3301 aaaattatga caagtagggg ggcagaactg gagttgggcg ccaatgctga agcaaacaac
     3361 ccaagcatca ttccagcaaa ggtggcaaga gtcatggtga tatatatctg gatgaactcc
     3421 atcactccgc ccggcattct aaatgaaata tagtggacca ccgtatatgc cactgcctga
     3481 tagagtgcca ataaagcagc gacccatacc tttgacaata catacggaaa aattttgaga
     3541 ttgaccaacc gctctcgccg ataaatatcc tgttccttaa cgatctcgcg catttgagaa
     3601 attccgccga ccataatgcc gtatatcgtt aagaggaaaa gagtgatcat gacttgagac
     3661 atgtcacctt tatagaaatc aaaaatctcc cgccccatta caaaagaaag gattacatct
     3721 aataaactta ccactggcgc agtggcaagc attaatccca gcgcaaaacg gtcacgggtc
     3781 aatattttga tattacgagc agataggatc attaactgtc ttaatgaaga gacctgtctc
     3841 gtattagctg ctttttgggg aagtctttcg ggctgtggta ccgtcgccac actttgtggt
     3901 gacaacttgc cagccaacgg ctttaagaca tattccgtgt gggcgttact tgagcgatat
     3961 cggttcgccc aatcttctgc tttgcctttg ctgggatcat ccaaaatggc atagatctca
     4021 tcgaactcga tcttgccggc tcggcgagcc cgctcagaac gatactggtc aaaataagct
     4081 agcgcttcat caggaggacc gaaccatgct aaataacctc ctcttgcgag aaagatcacc
     4141 ttatctgcca gcataacgtt ttttgtggca tgtgtaatta agatgatggt acgtccttgg
     4201 tctgccaggc gacgcatcaa ttgcattagc gctgtttctg taccggggtc caagcctgat
     4261 gtgggttcat caagaaagaa cagaccgggt ttggtcaaaa gttcaacacc aatcgagacg
     4321 cgtttttgct gcccgccgct gagtccgctg atctgaacgt ctttgcggtg ggtcaggtca
     4381 aggtcttcca aaacttccat aattcgtttg tgccgctctt ccggggaagt gtcggctggc
     4441 atgcgcaatt gagccgcata atcaagtgcc tgatagatag tcaactccat gtgaatgata
     4501 tctttttgtg gcacgaaccc aatctcattg cggatcgcgt caaaatgagt gtagatatca
     4561 atgtcattga cgtatacttt tgaaggagga gttgctggac ggtacccagc gactgcatcc
     4621 acaaatgtgg atttgcctcc gccgctctgc cctacaacaa cgatgaattc tctggggcta
     4681 aatgatgcat atatgttttg aagaatattg agatctttgc gaacccactt attcaaacca
     4741 actacatcca ctcttaagcc attcccgtca tcatatctgg caagttgatc cttgcccatc
     4801 acgaatcgat attgaccaat tcggatggaa tcgttgggct tcagccaaac cgagccttca
     4861 attcgttctc cattgacata ggttccatta gaactgcgga ggtcttctac ccgatagcgt
     4921 tgccctactt tttcgatctg ggtatggaat cgagatacat tgggggaggg gatgaccaca
     4981 tcattgctgg ggtcacgccc gatttgaatg agcgttttct cgccgaagac gatgtctcgc
     5041 tccatttgct gagatgcttc actgggcgca ttgtaggtta tagttaccat aaccccaggg
     5101 tccatactgc caattctcag cagatcattg tgatggagaa catgtgagcc gtctatcgga
     5161 cgcccttcaa attgaaccgg attttttgct tcaggcatca ccaccaactt gtacccgccg
     5221 cccttgacct gctcaagccg cgcatgccgg cttgagacaa tctgagatgg gatgacaatg
     5281 tcgttggtgt cgagtctgcc aatggatatg ctggaacctg ttaacgggta ggtttgtgtg
     5341 cttcctccgg caatactaac gattagctgg cgtggaccag cacttgcttg cggtattgct
     5401 ggtgcttcac caagcacagt ttgcatcaca tgtgcaggtt tagcaggacc ggaatgtttg
     5461 gcagttttgt tcgcgtcttt tatgctgaca acagttaggt tggaatcttc ttcagaaacg
     5521 ggagcggtat aaaccaggct gataatttgc cccaatcgaa ttttgtcacc tgattttagg
     5581 gtgtgttgct tatctagttt ttccccattg acatatgtgc cattactgct tcccagatct
     5641 tccagtacat aattttcgcc aatcaacatc agtctggcat gacggcgtga cacagcagat
     5701 gcgggtatgg tgatatccac actgtcttca cgaccaatga tgatttctga ctttgttaat
     5761 tcaaattctt tggaaggtgc actgctttga tcattgataa ggcttaattt gaaactgttc
     5821 acttgagtca aaggatgatc ctttcttgca aattttcaac acagtttctt gaatttatgg
     5881 ataagtgaga atttgtacag aattgagata attaagacaa gattatacct ttttattaaa
     5941 atgcatgtca acctaccaat gggggatacg taaaatcact gaaaatattg tgtttgtttt
     6001 cacttgcttt gctaaaaaat tggtctttta cgatggcttg aaaattttcg tttagcttga
     6061 atgttaataa tttcgcccta ctgatgggct atatagtttg gtgctataaa tgtgttacgc
     6121 ttaaatttta tgatataaat tcaaaatctt gttcttttaa tggtgaaaat acatgatgca
     6181 atcttcttct ttttcccgaa agctatccga agaaaacaat tcactggttc gtactctgcg
     6241 gaggcgggca gacttgcatc ctgaaaggct ggcgtttaga tttttacagg atgatgaatc
     6301 agaaaatatc accatcaact ttggggaact tgatcgtcgg gcacgtgcca ttggggcatg
     6361 gttagaaagc ttcaacgcaa aaggggagcg tgcacttatt ctctatccac ctggattgga
     6421 ttacatcgct gcttttatgg gctgcttata tgctggtgta atcgctgtcc ctgcataccc
     6481 gcctcgcctt aaccgtcctg taccccgtat ccaatctatc gttgacgatt cacatgcgac
     6541 ctttgcgctg actacaacgt cgatccattc gaacatcgaa aaacgatttg aacatgctcc
     6601 agacttgaaa gcgctacatt ggttaaacac cgagcaggta ccggcggggt tggaagcgga
     6661 ttggcgcgat cct
//
LOCUS       DAOQFT010000052         6616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5710_length_6616_cov_9.514251, whole genome shotgun sequence.
ACCESSION   DAOQFT010000052 DAOQFT010000000
VERSION     DAOQFT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5710_length_6616_cov_9.514251"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLR93_02255"
     CDS             <1..371
                     /locus_tag="PLR93_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH25809.1"
                     /translation="IANKNAFYVGITLLGFGTGISTIANLSLMFDLTVPEKVGLYIGA
                     WGFSNGLSRLVGLLMAGVIADVATKITGDALHGYLVVFGLEALMLFIAAIMLYRIDVG
                     VFQKKAHEPGFAEKIALASE"
     gene            399..1028
                     /locus_tag="PLR93_02260"
     CDS             399..1028
                     /locus_tag="PLR93_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH25810.1"
                     /translation="MTNDEWLSLAGAADLLGVHPSTVRLWSDKGLLPVHKTQGGHRRY
                     KRGEILLWAESNEKSKEEALEPEGMMQEVIKNVRMQISEGRLQEESWYQKLDEDARAQ
                     YRMSSRSLFQGLMIYVATNGREADSEAYAIGYEYASRARRYSLSYVDAAKAFLFFRDT
                     LVASVIKVYNEANVPAKRATEMYARMHTFTDEILISLLETYRKLETANH"
     gene            complement(1057..3582)
                     /locus_tag="PLR93_02265"
     CDS             complement(1057..3582)
                     /locus_tag="PLR93_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH25811.1"
                     /translation="MLSALLDFWKRDEETSPNIFAWQTLPPREAQTHPIPDDVPASLR
                     EALSTRGISQLYSHQQSAWTHARARRNVILATGTASGKTLAYNLPVLAKLIEDTQARA
                     IYLFPTKALTQDQQSSLESFQSSIANLKSSIYDGDTPQPQRSTIRKTARIILTNPDML
                     HTGILPHHSNWSDFFTNLKFIVIDEAHTYRGVFGSHVANVIRRLRRVAKFYGANPQFI
                     LASATIGNPKELAETLIEEPVELVDNDGSSRGERHFIIYNPPVLNPALGLRKSSILES
                     VRLANDLLKHDVQSVVFARTRRSVEILLTHLQTQIVNLKSKIENPIRGYRSGYLPTQR
                     REIEQGLRDGSVKTVVATNALELGIDIGGMGAALMVGYPGTIASARQQAGRAGRGDLP
                     AVSILVASPNPLDQFLAHHPEYFFDRSPEMALVNPNHLLILLEHLRCAMFELPFQRGE
                     GFGSISDELVDEYLEFLVSNQEAHFSNEKYFWMQDAYPAANISLRSASPQSVVLHTTA
                     DDGRPTIVGTVDGESANWMVHPGAIYMHEAQQYFVQELDLESHIAHLVPVGLDYYTEA
                     QQQSEIQITALNEQLATRGCEKGYGEIQVTTQVVGFRKIRWFTNENLGQEPLDLPPSE
                     LQTTGYWIVLSEAALKVLRDAGLWTNDPNDYGPDWQKIRLAVRKRDQFKCQVCGAAET
                     NREHDVHHKTPFRAFIQNGVLDREQANRLENLTTLCQSCHRKVEQNVRIRSGLTGLGF
                     VLGNLAPLFLMCDAGDLGVFSEPAWSAVNGQPAVVLYDLVPAGIGFSEKLFELHDELI
                     RRAHELVSECACEDGCPSCVGPGGENGYGGKAETLAILQQLLK"
     gene            complement(3588..4598)
                     /locus_tag="PLR93_02270"
     CDS             complement(3588..4598)
                     /locus_tag="PLR93_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNH25812.1"
                     /translation="MNKFLVFLFLFLGVVVVVFSFGELETILQTLQKAHLGFFLLAVL
                     IQIAWFVSSGRMYQSIFHLLGIHEDVLTLSRIAAAANFINVVAPTAGAGGVALFAAEA
                     KRRGHPAGKATVAAALFLLLDLAAFLIILSLGLIVLVRRNDLDAGEISASLILFTTAA
                     GFTSVLYIGYRSAEKLGNILAKLARGINKVVHLFRKENYLSEERAHEFAHELADGFSG
                     LTEQPSSLIRPVLWGLFEKGLLMLILMCSFLAFEVSFSAGTIIAGFAIAYLFLIVSPT
                     PSGIGIVEGIMPVALSSLNVNWSQGVVITLLYRAVTFWVPLGIGAWAFRTLHKERAEV
                     AE"
     gene            complement(4745..5437)
                     /gene="cysE"
                     /locus_tag="PLR93_02275"
     CDS             complement(4745..5437)
                     /gene="cysE"
                     /locus_tag="PLR93_02275"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNH25813.1"
                     /translation="MFKTIREDVQSVLDRDPAARNVLEVLLCYPGVHAIWYHRLSHKL
                     WKGGFKLLARWVSQFARGLTGVEIHPGAKIGKNFFIDHGMGVVIGETAEVGHNVTLYH
                     NVTLGGTSSEKVKRHPTLEDNVVVGAGAKVLGAIVIGEGSRIGANAVVVKSAPPNSVI
                     VGVPGQVLLRSHTNKQIDLNHNLLPDTIGDTVAALVAHVNELEKKMNTHFMGSPLHRS
                     EHGVWHGEDFAI"
     gene            complement(5494..6432)
                     /gene="cysK"
                     /locus_tag="PLR93_02280"
     CDS             complement(5494..6432)
                     /gene="cysK"
                     /locus_tag="PLR93_02280"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNH25814.1"
                     /translation="MKIASNVTELIGNTPLVRLNRVTEGAKAEVVAKLEFFNPAHSVK
                     DRIGFSMIDAAEKAGLINKDTVLVEPTSGNTGIALAMVAAARGYKLILTMPETMSKER
                     RMLLRAYGAELVLTPGPDGMGGAIRKAEEIVASDPKKYFLPQQFKNPANPEVHRKTTA
                     EEIWRDTDGKVDYLVAGIGTGGTITGVGEIIKERKPSFKAIAVEPDASPVLSGGAKGP
                     HPIQGIGAGFVPDILNTKIYDEIIRVKADDAFQTARRLAREEGLLVGISSGAATWAAL
                     QVANRAENAGKLIVVIIPSFGERYLSTALFADLVDN"
BASE COUNT         1705 a         1635 c         1677 g         1599 t
ORIGIN      
        1 tgatcgccaa caaaaatgcc ttttatgttg gcattaccct gctcgggttt ggcaccggaa
       61 tttccaccat cgccaacctc tccctcatgt tcgacctgac cgtccccgaa aaggttggtt
      121 tgtatattgg tgcttggggc ttctcgaacg gactctcgcg tttagttggt ctactcatgg
      181 caggcgtcat cgcagatgta gcgaccaaaa tcactggtga tgcccttcat ggctatctgg
      241 tggtctttgg gttagaggca ttgatgttgt tcatcgctgc gatcatgttg tatcgcattg
      301 atgtgggcgt tttccaaaag aaggcgcatg aacctgggtt tgctgaaaag atcgcgcttg
      361 cctcagaata gtttgaaaaa gcaggatatc ggctgaacat gacaaacgat gaatggcttt
      421 ctctcgctgg tgccgccgat ctgctggggg ttcacccgag cacggtgcga ttatggtctg
      481 ataaaggctt actccccgtt cataagactc agggtgggca tcgccgctat aagcgtggag
      541 aaattctcct ttgggcagaa tcaaatgaaa agtccaaaga agaggcgctt gaacccgaag
      601 gcatgatgca agaagtcatc aagaacgtcc gcatgcaaat ctctgaagga cgacttcaag
      661 aagaatcctg gtatcaaaaa ttggatgaag atgcccgtgc acaatatcgc atgagttcgc
      721 gttcgttgtt tcaagggttg atgatctatg ttgcgaccaa tgggcgtgaa gcagactcgg
      781 aagcctatgc tattgggtat gagtatgcct cgcgggcgcg gcgttacagc ctcagttatg
      841 tagatgctgc caaagcattt cttttctttc gagatacctt ggtagcttcg gtcatcaagg
      901 tatataacga agccaatgtt ccggcaaagc gcgccacaga aatgtacgca cgtatgcaca
      961 ctttcactga tgaaattctc atcagcttgt tggaaaccta tcgcaagctg gaaacggcaa
     1021 atcactaaaa aggacgagcg taggctcgtc ctttttttat ttcaataact gttgcaagat
     1081 cgccagcgtt tctgccttgc ccccatatcc attttcgccg ccgggtccta cgcatgatgg
     1141 gcaaccatct tcgcaggcac attcactcac caattcatgc gctcgtcgaa tcagttcatc
     1201 atgcagttca aataattttt cgctgaagcc aatccccgcc ggaacaaggt cgtacaacac
     1261 cactgctggt tgaccattca ccgcagacca agccggttca gaaaagactc ccaagtcacc
     1321 ggcatcacac atcaaaaata acggcgcgag attaccgagc acaaatccca atcctgttaa
     1381 tccgcttcga atgcgcacgt tctgttccac tttgcgatgg cagctctggc aaagcgtggt
     1441 gagattctcc aaccgattcg cctgttcacg gtctaagact ccgttttgga tgaaggcacg
     1501 gaagggagtt ttgtgatgca catcatgctc gcgattcgtc tccgccgcgc cgcacacctg
     1561 acatttgaac tggtctcgtt ttctgacggc gagtcttatc ttttgccaat cgggtccgta
     1621 gtcgttcggg tcattggtcc atagaccagc atcacgcaac actttaagcg ctgcttctga
     1681 gaggacgatc caatagccgg tcgtttgtaa ttcagaaggc ggaaggtcaa gcggttcctg
     1741 cccaagattc tcattcgtga accagcggat cttgcgaaag cctacgactt gtgtcgtgac
     1801 ttgaatctcg ccatatccct tttcacagcc gcgggtggca agttgctcat tcaatgctgt
     1861 gatctgaatt tcagattgct gttgggcttc ggtgtagtaa tccaagccaa ccggcacaag
     1921 gtgtgcgata tggctctcaa ggtcaagttc ctgcacaaag tattgttggg cttcgtgcat
     1981 atagatcgcg ccggggtgta ccatccaatt ggcagactcg ccatctacag ttcctacaat
     2041 ggtggggcga ccatcatctg cagtggtgtg cagcaccaca ctttgcgggg aggcagaacg
     2101 caaagaaata ttcgcggcgg gataggcatc ttgcatccaa aaatattttt cgttcgagaa
     2161 atgcgcttct tggttggaga ccaaaaactc aagatactca tccaccaatt catcagaaat
     2221 ggagccgaag ccttcgccac gctgaaatgg cagttcgaac atggcgcaac gtaaatgctc
     2281 cagcaaaatc aacaaatgat tcgggttgac caatgccatc tccggcgaac ggtcaaaaaa
     2341 gtattcggga tgatgcgcga gaaattgatc caacggattc ggtgaagcga caaggatcga
     2401 aaccgcaggc aggtcaccac gtccagcccg ccctgcttgc tggcgcgcac ttgcaatggt
     2461 gccggggtag ccaaccatga gggcagcccc catgccgcca atgtcaatac cgagttcgag
     2521 cgcattggtt gccacaacgg tcttgaccga gccatcacga agtccctgct cgatctcgcg
     2581 gcgttgagtt gggaggtagc cagagcgata gccgcgaatc gggttttcga ttttcgattt
     2641 cagatttacg atttgtgttt gtagatgggt tagaaggatt tccacactac ggcgtgtccg
     2701 cgcaaagacg accgactgca catcatgttt cagcaaatca ttcgccaagc gtaccgattc
     2761 aaggatgctg ctcttgcgca agcccaatgc aggatttaac acaggcgggt tatagataat
     2821 gaaatgacgc tcgccgcgag aggaaccgtc gttatccacc aattcaacgg gttcttcaat
     2881 aagcgtctcg gctaattctt ttgggttgcc aatggttgcc gaagcaagga tgaactgcgg
     2941 attcgcgcca tagaattttg ccacccgtct gagcctgcgg atgacgttgg cgacatgcga
     3001 accgaagact ccgcggtagg tgtgggcttc gtcgatgacg atgaatttga ggttggtgaa
     3061 aaagtcgctc cagttggaat gatgcggcag aatgcccgta tgcagcatgt ctggattcgt
     3121 gagaatgatg cgtgcagttt tgcgaatggt agagcgttgt ggttgaggag tgtcgccatc
     3181 atagattgat gatttgagat ttgcgattga cgattgaaaa gactcgaggc tcgattgttg
     3241 atcttgagta agggctttgg tggggaagag atagattgcg cgtgcttggg tgtcttcgat
     3301 caactttgcg aggacaggca agttgtaagc aagagtcttt ccgctggcgg tgccggtggc
     3361 gaggatcaca tttcggcggg cgcgggcatg agtccacgcg gactgttggt gggaatagag
     3421 ttgggaaatg ccgcgggtag ataaggcttc tcgaagcgag gcaggcacat cgtctggaat
     3481 gggatgcgtt tgagcttcac gtggcggaag cgtttgccag gcaaagatat tgggtgaggt
     3541 ttcttcgtcc cgcttccaaa aatccagaag agcagagagc atgaacgtta ctcggcaacc
     3601 tcagcgcgtt ctttatgcaa ggtgcgaaat gcccaagcac caattcccaa tggcacccaa
     3661 aatgtgaccg cgcgatagag cagtgtgatg accacgccct gactccagtt aacgttcaaa
     3721 gagcttaagg caaccggcat gatgccttcg acaataccaa tgccagatgg ggtaggcgag
     3781 acgataagaa agaggtatgc aatggcgaaa ccagcaatga tggtgccagc tgaaaatgaa
     3841 acttcgaagg caagaaatga gcacataaga atcaacatca acaaaccttt ttcaaacaag
     3901 ccccacaaca ctgggcgaat aaggctggag ggttgttctg ttaaccctga aaacccatcg
     3961 gcaagttcgt gtgcaaattc atgggctcgc tcttcgctaa gataattttc ttttcggaaa
     4021 agatgtacga ccttgttgat gccacgcgca agtttcgcaa gaatgttccc caatttttca
     4081 gcagagcgat acccgatgta taaaacagat gtaaagcctg cggctgttgt aaataaaatc
     4141 agagaggctg agatctctcc ggcatccaag tcatttcggc gaacaagcac gatcaagcca
     4201 agtgagagga taatcaagaa agcagctagg tctaatagga ggaacaatgc agctgcgaca
     4261 gtcgctttgc cagcaggatg cccacgccgt ttcgcttcgg cagcaaagag cgcgactcct
     4321 cccgcgccgg cagtgggtgc cacgacattg ataaaattcg ccgctgcggc aatgcggctg
     4381 agagtgagca catcttcatg aatccccaat agatgaaaga tggattgata catccgcccg
     4441 ctggaaacaa accaagcgat ctgaataaga actgccaata gaaaaaaacc aaggtgtgct
     4501 ttttggagag tctgaagaat cgtctccaac tcgccaaagc tgaacacgac cacgactaca
     4561 cccagaaaaa ggaacaagaa aaccagaaat ttattcatgt atacctatat acctacacag
     4621 acattgtaac aaaacattga ccctttctgt acgggagcaa aagattcatg acagttataa
     4681 aaatagaccg ttgccgcgtg ttttatacgc aggaacggtc tattatgttg cggaagatca
     4741 aaacctaaat ggcaaaatct tcgccgtgcc atactccatg ctcagatcga tgcaaaggag
     4801 accccataaa gtgagtattc attttctttt ccaattcgtt cacatgtgca accaaagccg
     4861 caactgtatc accgatggta tctggcaata agttgtggtt gaggtcgatc tgtttattgg
     4921 tatggcttcg caaaaggacc tgccccggaa ccccaacaat gaccgaatta ggcggcgcgg
     4981 acttaacaac aacagcattt gcaccaatcc ggctgccttc tccaataacg atcgcgccca
     5041 aaacttttgc accagcgcca acgactacat tatcttccaa tgtcgggtgg cgtttcactt
     5101 tttcagaact ggtgccgccc agagtcacgt tgtgatacaa ggtgacatta tgccctactt
     5161 cagccgtctc accgatcaca acgcccatgc cgtgatcgat aaagaagttc ttaccgatct
     5221 ttgcgccagg gtgaatctcc acaccggtca gtccgcgtgc gaattgagag acccagcgtg
     5281 caagcagttt gaaaccgcct ttccaaagct tgtgtgaaag gcggtgatac caaatggcat
     5341 gtacgcccgg gtaacacaat agcacctcga gcacattgcg ggctgcgggg tcacggtcca
     5401 gcacagattg aacatcttca cgaattgtct tgaacattga aactcacttt acgagaaaac
     5461 catcacgctc tcttaattga gcgctttaaa atgttagtta tcgaccaagt ctgcaaacaa
     5521 agcggtggac aagtaccgtt ccccaaacga agggatgatg accacgatca atttgcctgc
     5581 attttcagcg cggttggcaa cctgaagcgc agcccatgtc gctgcacctg aagaaatccc
     5641 taccagcaac ccttcttcac gtgccaagcg gcgagcggtc tgaaaagcat catctgcttt
     5701 gacacgaatg atctcatcat aaattttggt attgagaata tcgggcacaa accctgcacc
     5761 aatcccttga atcggatgcg gtcctttggc gccgccagaa agaacggggg aggcatctgg
     5821 ctctacagca atggctttga aggaaggttt gcgttctttg atgatctcgc ccacaccagt
     5881 gatggttcca cctgtgccaa tgccagccac aagataatct actttgccgt ctgtgtcgcg
     5941 ccagatctct tccgcggtgg tcttgcggtg aacttcaggg ttggcaggat tcttaaattg
     6001 ctgcggcaga aaatacttct tggggtctga ggcgacaatt tcctccgcct tgcgaatcgc
     6061 accgcccatg ccatcgggcc cgggggtgag caccaactct gctccatacg ctcgcaacaa
     6121 catgcggcgc tccttgctca tggtttcggg catggtgaga atgagtttgt atccacgtgc
     6181 tgccgcgacc attgccaagg caatgcctgt gtttccagat gtgggttcta cgagaaccgt
     6241 atctttatta ataagacctg ccttttcagc agcatcgatc atcgagaaac cgatgcggtc
     6301 ctttacgcta tgggctgggt tgaaaaactc gagctttgca accacttctg ctttggctcc
     6361 ttcggtaacg cgattcaagc gcaccaatgg agtattgccg atcaactctg tgacgttact
     6421 tgcgattttc attttaagac tcctctgtgt tttggttttt aaaaataaaa aaacggctgt
     6481 ctgcctgctg aagtaattca gcattggcat ccgatacaaa ttcaaaaaac tggtcaagcc
     6541 agttttgttg aaaatgcggg gctcaagccc accggatgcg gcagtcagcc gttgatttct
     6601 gggaaaatcg gcggtg
//
LOCUS       DAOQFT010000053         6549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5782_length_6549_cov_6.858639, whole genome shotgun sequence.
ACCESSION   DAOQFT010000053 DAOQFT010000000
VERSION     DAOQFT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5782_length_6549_cov_6.858639"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..640)
                     /locus_tag="PLR93_02285"
     CDS             complement(41..640)
                     /locus_tag="PLR93_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNH25815.1"
                     /translation="MMLFLRAFIIGISIAAPVGPIGVLCIRRTLANGRLAGFVSGMGA
                     ASADMVYGAIAAFGLTAITGLLVDNAFWFRIVGGLFLLYLGIKTLLEKPSDKPAQAAQ
                     GNLLSMYTSTFLLTITNPMTILSFAAIFAGTMLGKTTGSPLVMVAGVFAGSAAWWLTL
                     SFIVGILRDRLTQVHMAWINRISGAIIIAFGVYALFSGS"
     gene            complement(756..1700)
                     /gene="secF"
                     /locus_tag="PLR93_02290"
     CDS             complement(756..1700)
                     /gene="secF"
                     /locus_tag="PLR93_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNH25816.1"
                     /translation="MNIIKNRYLYFLISLLVIIPGLVFMGLNWANTKQGPLVLGIDFR
                     GGSLLEIQFEGTRPSSSDIAALYAEFSTEEENFKDPVIQPLGEDAYSIRSKTMTDETK
                     GKLVAEMESRFGSKITVLNFTSVSASVGAEVTRAAGVAIFMAAAAILIYIWWAFRSVE
                     HAYRYGTAAIIAMLHDVFVVLGAEAILGYFFGWEADALFLTALLTVIGFSVHDTIVVF
                     DRVRENSNLGRKIGYESMINHSIVQTLDRSLTTQLTVMFTLFALSLFGGDSIRHFTII
                     LLVGILSGTYSSIFNAAPILVVWENREWTTWFGRKASA"
     gene            complement(1710..3077)
                     /gene="secD"
                     /locus_tag="PLR93_02295"
     CDS             complement(1710..3077)
                     /gene="secD"
                     /locus_tag="PLR93_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNH25817.1"
                     /translation="MRNKNTWLIVILFLLAFSLWVNFSKTITVINPFNDNTPVVERDV
                     DIKLGLDLRGGLQALLEADVPADSTVSSADMENAKNILQNRANALGVSEIVMQTAGDR
                     RIVAEFPGVTNPEEVVSALKQTALLEFVDMGTTPVAEGTTIQTDFAAGDTTAAATPAP
                     DGTTPVIYHTVMTGTSLDTASVVRSQTGEYEISFTLKADAASVFGEYTTTHTNQYLAI
                     VLDKVVISTPVIRQPITSGQGVISGNFTSESAQTLAIQLRSGALPIPIKVVESRTVGP
                     TLGEESVRKSVLAGLIGLTVVVLFMGFYYRLPGVIADLALICYAIFSLMLFKLIPVVL
                     TLPGIAGFILSVGMAVDANVLIFERLKEELRAGRNLRHAIDLAWSRAWSSIRDSNTST
                     LITCVILFIFGSTFGASMVKGFAVTLAIGVLVSLFTAITVTRTFLHAILDNVKNTEHP
                     SWFGL"
     gene            complement(3242..3601)
                     /locus_tag="PLR93_02300"
     CDS             complement(3242..3601)
                     /locus_tag="PLR93_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25818.1"
                     /translation="MDSDNSSLIGVVGPCGSGKSTLIRGLEAHGYRCRHIAQEHSYAK
                     QMWQIITKPDILIFLECSFENSTQRRKLNWVAADHEEQLRRLSHAYEHAHIIINTNTL
                     DENGVLAQALDYLKKHL"
     gene            complement(3591..4652)
                     /locus_tag="PLR93_02305"
     CDS             complement(3591..4652)
                     /locus_tag="PLR93_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNH25819.1"
                     /translation="MHKKFHFIDKHRWRHIQAGIRDTTILLREFRTPLFWFTFAVVGG
                     GILYHYLSQRLNEPVYSLTESIYIVLIATFLQPANREFPHHILLQIFHFVMPVIGLSV
                     LAQGLADFGSLFFNRKSRNKEWEMAVASTMQNHIILVGLGHLGYRVALKLHEMGEPLV
                     VIEFNADADTVNTIKNMGIPVIHDDGTRPAVLEAANIKKSRTIIMASQNDSMNLQIAL
                     KARSLNPNIQVVIRIFDDDFAHALQEQFSFIALSATEMAAPVFASAAAGVDVTNPISV
                     EGQLLSLARLTISKNAPFADKTIGYIEDTYHLSIVLHRKNGHSEMHPTDKTAIHPSDV
                     IAILGGPEQLNKIFHDNGQ"
     gene            complement(4645..5352)
                     /locus_tag="PLR93_02310"
     CDS             complement(4645..5352)
                     /locus_tag="PLR93_02310"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH25820.1"
                     /translation="MEIQIAVAKINKYAVSESGDTLEVIERPTGGLSVVLADGQTSGR
                     GAKAVSMMVVRKVISLIAEGVRDGAAARAASDALFTERAGKVTCTLNIASVDLHSKTI
                     VLTRNNPAPMYVCRGEEIEIHNEESISLGTSRNLRPIITELNIEAGLTIIIFSDGISH
                     AGERRGTPLDVRHTLLSMLEDQDPSPQQLADTLLAEAVRLDDKRPADDISVLVLRVSP
                     RTGDDVRRMAVRLPINA"
     gene            5481..5681
                     /gene="rpmF"
                     /locus_tag="PLR93_02315"
     CDS             5481..5681
                     /gene="rpmF"
                     /locus_tag="PLR93_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNH25821.1"
                     /translation="MTPHPKRKHSKGRRDRRRSQDALSAANLVTCSNCGNKRLPHVVC
                     PNCGYFKGREVVQVKKEKKSAE"
     gene            complement(5747..>6549)
                     /locus_tag="PLR93_02320"
     CDS             complement(5747..>6549)
                     /locus_tag="PLR93_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25822.1"
                     /translation="DNRWLASASFDQTVKIWDTTTHELITEPLQHDSGVYTLAFNPNG
                     SSLYVAGFPFNIFSYSLTDTSNIPEPQQLTGHGSAVNLLASNPNYPEFIASTSDDKTL
                     LVWNVANGEHTPPVLGLQESMEAVAFRPSGDWLASATNNNTVLLWQFDAKRCAKEWDQ
                     DTCQPQIIGSPLTGSQTAVYNVIFLSDTVMISSSQGGQLIKWNLDKAYWYDVACSIVN
                     RPLSEAEHRQYVGKQLNSKLLNAITWVSERFGNDVPPAPACLNIDGNN"
BASE COUNT         1772 a         1487 c         1702 g         1588 t
ORIGIN      
        1 actaataaaa agtgccagcg atatcgctgg cactttttat ttagcttccg ctaaacaagg
       61 cgtacactcc aaaagcaatg atgattgcac ctgaaattcg gttaatccac gccatatgaa
      121 cttgggtcag ccgatctcgc agtatgccaa caataaaact aagggtcagc caccaagcag
      181 cggacccagc aaatacgcct gcaaccatga ccaaaggaga cccggtcgtc tttccgagca
      241 tggttccagc aaagattgct gcaaacgaga gaatggtcat agggtttgtg atcgtcagca
      301 gaaatgtaga tgtgtacata ctgagcaggt tgccttgcgc tgcttgagca ggcttgtcag
      361 aaggcttctc gagcaatgtc tttatgccta gataaagcaa aaacaaccca ccaacgatac
      421 gaaaccagaa ggcgttatct accaataatc ccgtaatcgc agttaatcca aaagcggcaa
      481 tggcaccgta aaccatatct gcactggcgg cacccatgcc agaaacaaaa cctgccaacc
      541 taccgttcgc taatgtgcgg cggatgcata acacgccgat gggtccgacc ggggcagcaa
      601 ttgaaattcc aataataaaa gcacgaagaa aaagcatcat gaatcaccta caagcaacgc
      661 gatatgcgtc taattactaa tttgaataaa gtaagaaatc atcctttgaa ataaaaaaac
      721 agccatcaaa cctgatggct gttttgaagc gtattttacg cgcttgcttt tcgaccgaac
      781 caggttgtcc attcgcgatt ttcccaaacg acaagaatgg gcgccgcatt gaaaatggaa
      841 gagtaggtac cgcttaagat acccaccaac aagatgatgg tgaaatgccg aatgctatcg
      901 ccaccaaaaa gcgagagggc gaaaagcgtg aacataacgg tcaactgggt ggtcagagag
      961 cggtccaagg tttggacaat ggagtgattg atcatggatt cataaccaat tttgcgacca
     1021 agattggaat tttcgcgaac tctgtcgaac accacaattg tgtcgtgaac tgaaaaaccg
     1081 attacggtga ggagcgcagt gaggaagagc gcatctgctt cccagccgaa gaaatagccc
     1141 aaaatggctt cggcaccaag cacaacgaat acatcatgca acatggcgat gattgccgct
     1201 gtgccgtagc gataggcgtg ctccacactt cggaacgccc accaaatata gatgaggatc
     1261 gcagccgctg ccatgaaaat agcaacacct gcagcacgag tcacctcagc accaacagag
     1321 gcgctaacag aggtgaagtt caacacagtg attttggacc caaaacgaga ttccatttca
     1381 gcaacaagct tgcctttggt ttcatcggtc atggtctttg agcgaatgga ataggcatct
     1441 tcaccgagcg gttgaataac ggggtctttg aagttttctt cttctgtaga gaattcggcg
     1501 taaagagcag caatgtctga agatgagggg cgagtgcctt caaactggat ctctaataag
     1561 gagccgcctc ggaaatcaat tccaagaact aacggaccct gtttcgtgtt tgcccaattt
     1621 agtcccatga agaccaaccc tgggataatc acaagcaggg agatcagaaa gtatagatag
     1681 cgattcttga ttatgttcat ggtattgtcc taaagcccaa accagctggg gtgttcggta
     1741 ttcttaacat tgtctagaat ggcatgcagg aaggtgcgtg ttacggtgat ggcagtgaag
     1801 aggctgacga gcacgccaat cgccaaggta actgcaaaac cttttaccat gcttgcgcca
     1861 aaggtgctgc cgaaaatgaa caagatgaca catgtgatga gtgtcgatgt gttggagtct
     1921 cgaatggaag accaagcacg gctccaagca agatcgatgg cgtgacgcag attgcgtcca
     1981 gcgcgtaatt cttccttcaa gcgttcaaag atcaacacgt tggcatctac tgccataccc
     2041 acagaaagaa taaagccagc gataccgggc aaggtcaaca ccacggggat caacttgaaa
     2101 agcatcaaag agaaaatggc gtaacaaatc aacgcaaggt ctgcaatgac gccggggagg
     2161 cgatagtaaa agcccatgaa gagaaccaca acggtcaacc caatgagacc tgccaacacg
     2221 ctcttacgca cactttcctc accgagggtc ggaccaactg tgcggctttc aacaaccttg
     2281 atcggaatgg gcaaggcacc agaacgaagc tgaatggcga gggtctgggc agattcggaa
     2341 gtgaagttcc cagagataac gccctgtccg cttgtgatgg gttggcgaat tacaggggta
     2401 gaaatcacaa ccttatccag cacgattgca agatattgat ttgtatgggt tgtggtgtat
     2461 tcaccaaaga cactagcggc atcagccttc aacgtgaatg aaatttcata ttcaccggtc
     2521 tggctgcgaa caacacttgc tgtatccaag ctggtaccgg tcatcacggt gtgatagatc
     2581 acaggtgtcg tgccatcggg ggcgggggta gcagcagccg ttgtatcgcc tgccgcgaag
     2641 tcggtttgaa tggtggtacc ttcagctacc ggggttgtgc ccatatcaac aaactctagc
     2701 aaagccgttt gcttcaaagc gctaaccact tcttctgggt tggttacacc cggaaattca
     2761 gccacaatac ggcggtcgcc ggcggtctgc atcacaattt cgctcacgcc aagggcgttc
     2821 gcgcggtttt gcagaatgtt cttggcgttt tccatatctg cgctgctaac cgttgaatcg
     2881 gcagggacat cggcttcgag aagcgcttgc aagccgccac gcaagtcgag accaagttta
     2941 atgtcaacat ctctttcaac tacaggggtg ttgtcgttaa agggattgat aactgtgatg
     3001 gttttgctga aattaaccca aagggagaat gccagcagaa agaggatcac aatcagccat
     3061 gtattcttat tacgcatgtg tttactccat ccacgaaaat gccagccttt ggctggcgcg
     3121 gacgacatta tactctggtt gattgatttt ttggaggaag aaggacccgc ctccagcgtg
     3181 ggaaaggctt ggtctgtgga cccaaacagg gtccacgcct gaggcggggc aaggcttggg
     3241 gctagaggtg cttctttagg tagtctaggg cttgcgcaag gactccgttt tcgtccagcg
     3301 tgttcgtgtt aataatgata tgagcgtgtt cgtaagcgtg agaaaggcgg cggagttgtt
     3361 cttcgtggtc ggcagctacc caattgagct ttcggcgctg agtggagttt tcgaaagaac
     3421 attctagaaa aatgaggata tcgggtttgg tgatgatctg ccacatctgt ttggcatagg
     3481 aatgttcttg tgcaatatgc cggcagcgat agccatgcgc ctccaacccg cgaatcaatg
     3541 tggatttgcc cgaaccgcac ggacctacga caccgattaa tgaggaattg tcactgtcca
     3601 ttatcgtgga atattttgtt caattgttct ggcccgccaa gaatggcaat tacatcgctg
     3661 gggtggatgg cagttttgtc ggtggggtgc atttctgaat gaccattttt gcgatgcaac
     3721 acaatactaa gatggtaggt atcttcaata tagccgatag ttttatctgc aaaaggagca
     3781 ttctttgaaa tggttaggcg tgccaggctt agcagttgac cttcgactga aattgggttg
     3841 gttacgtcta cgccagcggc ggcagatgca aagacaggcg ccgccatttc ggtagcgcta
     3901 agagcaataa atgagaattg ttcttgaagc gcgtgagcaa agtcatcgtc aaagatacgg
     3961 atgactacct gaatgttggg gttgagcgag cgagctttga gcgcgatctg taagttcatc
     4021 gagtcatttt ggcttgccat aataatggtg cgcgactttt tgatattcgc ggcttccagc
     4081 acggctggac gggtgccatc atcatgaata acaggaatgc ccatattttt aatcgtattg
     4141 actgtatcag cgtctgcatt gaattcgatg acgaccaaag gttcacccat ttcgtgtagc
     4201 tttagagcga cgcgatagcc aaggtgccct aaaccaacga ggatgatgtg gttttgcata
     4261 gtggatgcga ctgccatttc ccattccttg ttgcgtgatt ttctattgaa gaacaaactt
     4321 ccgaaatctg ccaacccttg tgcaagcacg cttaagccaa tgactggcat cacgaagtga
     4381 aaaatttgaa gaaggatgtg atgcggaaac tcgcggttgg caggctgtaa aaatgtagcg
     4441 ataagaacga tgtagatgga ctcggtgagg ctgtacacag gttcgttcaa acgctgtgat
     4501 aaatagtgat agagtattcc gccgccgact acggcgaagg tgaaccaaaa aagaggtgtg
     4561 cggaattcac gtaaaaggat ggtggtatca cgtatcccag cctgaatgtg acgccaacga
     4621 tgtttatcga tgaaatgaaa ttttttatgc attgatgggc aggcgcactg ccatgcggcg
     4681 gacgtcgtct cctgttcgtg gagaaacacg cagaacaagt acactgatgt catctgctgg
     4741 gcgtttatcg tccagacgga cggcttcagc cagcagagta tctgccagtt gttgtgggga
     4801 aggatcttgg tcttctagca tggagagaag ggtatgacgt acatccaaag gggtgccgcg
     4861 cctctcgccg gcatggctaa taccatctga aaagatgatg atggtcagcc cagcttcgat
     4921 gtttagttcg gtaatgatgg ggcggaggtt gcgcgatgta cccagtgaaa tgctttcttc
     4981 gttgtgtatt tcaatttcct cacctctaca tacgtacatg ggagcggggt tgttgcgggt
     5041 gagaacaatg gttttactgt gtaaatctac agaggcgata ttcagagtgc aggtcacttt
     5101 accggctcgt tcagtaaata gcgcatctga ggcggcgcgg gcggcggcac cgtcacggac
     5161 gccttcagca atgaggctga tcactttgcg gacgaccatc atggagacgg ctttggcgcc
     5221 acgcccgctg gtttgaccat ctgccaaaac gacagataac ccgccggtgg ggcgttcgat
     5281 gacttcgagc gtgtccccgc tttcggaaac cgcatatttg ttgatttttg ctacggcgat
     5341 ctggatttcc atatagagga ttttacatcg aattcgcctt gacgtcactg cctcccccat
     5401 atataatcag cgggcttcgt aataaaacca atccagacgg gcgtgcgccc gtctgatgta
     5461 tgtataaagg agaacgtttc atgaccccac atccaaagcg caaacattca aagggacgcc
     5521 gggatcgccg ccgctctcag gatgccttga gcgccgccaa cctcgttacc tgttccaact
     5581 gcgggaacaa gcgcctgccg catgtcgttt gcccgaactg cggatatttc aaaggacgcg
     5641 aagtggttca ggttaaaaaa gaaaagaaat cggctgaata aattgcaacg ggtcgtgaac
     5701 ctcacgaccc gtttgtgttt aattcagcat ctttttattc ggtcagttag ttatttccat
     5761 caatattcaa acatgcaggg gctgggggga catcatttcc aaaacgttca gaaacccagg
     5821 tgatcgcatt caataatttt gaatttagtt gcttaccaac atattggcga tgttcagctt
     5881 cgcttaaggg acgatttaca atgctacagg caacatcata ccaataggct ttgtctaagt
     5941 tccatttgat caactgtccg ccttggctgg atgagatcat gactgtatct gataaaaaga
     6001 tgacgttata gacggcggtc tgacttcccg tgagcggcga gccaataatt tgaggctggc
     6061 aggtgtcttg gtcccattct ttggcacagc gttttgcatc aaattgccag agcaagacgg
     6121 tgttattgtt cgttgcgctg gcgagccaat ctccgcttgg acggaaggcg acagcttcca
     6181 tgctttcttg caaacctaat acgggtggag tgtgttcacc attcgctaca ttccaaacta
     6241 acaacgtttt gtcatcgcta gtggaggcga tgaactcagg ataattagga ttagaggcaa
     6301 gtaaatttac ggcagagcca tgaccagtga gttgttgagg ctctggaatg ttggaagtat
     6361 cggttaggct gtaggaaaag atattgaagg ggaaaccagc cacatacaag gaagaaccgt
     6421 ttggattgaa agcaagggta tatactccag agtcgtgttg caatggttct gtgatcaatt
     6481 catgagtcgt tgtatcccat atctttacgg tttgatcaaa gctggcagag gcaagccaac
     6541 gattatctg
//
LOCUS       DAOQFT010000054         6548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5785_length_6548_cov_4.909903, whole genome shotgun sequence.
ACCESSION   DAOQFT010000054 DAOQFT010000000
VERSION     DAOQFT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_6548_cov_4.909903"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLR93_02325"
     CDS             complement(<1..758)
                     /locus_tag="PLR93_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25823.1"
                     /translation="MSRIVAGYIFSAALTVALLFSVFAWQRILTIVLLFIWFTFIILF
                     FFKNLLRVAQDPNTENILDAQRARVVLFSMILAEVPLWLRPLTLALGLDIFSYNLLLV
                     FQLFVPLGIAYAVLRHDLFGIDRALRRTLVYGTVSLLLLTLYLALTTSLTSLFADSLT
                     SKPLAPAISLLAAAILFDPTRKFIQRWLDKLLYPDRLKFESAIQSMQYLLARANRRDE
                     IIHLLNDVFPNQIGAEWGMLKLFPEPDVPPPHTFP"
     gene            complement(1208..2038)
                     /locus_tag="PLR93_02330"
     CDS             complement(1208..2038)
                     /locus_tag="PLR93_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25824.1"
                     /translation="MGIETDGPDGVVGDLPNNPGGGTGTYTVVSISGNPVYVSLTPDG
                     NYDLAFYEYLTNFGGTPHVELDNIIIGISNDSNGATYYEVFNWGNNIADTNTTANTNT
                     LPDQGPCASNVECDNRVIPLGSLYTDPATGITTGILIDVDTALSAAPGDVYYNYIVII
                     SPNLGTGDVTQVDAIVVTEIPIPTPPPGPVADLPAQPTVNEVTVPDTNPTPSLEAIIT
                     ELVTAIEDLVTSTEETVPTPSPEEINTDQVPTESISTDETIDATPVEETSVPQETATP
                     "
     gene            2088..2570
                     /locus_tag="PLR93_02335"
     CDS             2088..2570
                     /locus_tag="PLR93_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25825.1"
                     /translation="MAVAVAVVVEVVVVVDVAVVVEVAVVVDVAVVVEVAVVVDVAVV
                     VEVAVVVDVAVVVDVAVVVEVAVVVAVTVTVGVSVIVEVGVTVGVSVGSSVSVSVGVV
                     VTVLVEVAVAVVVTVEVAVGVVSVAVVVAVGVTSVGVGVTVGSKVKKGGYSISPSGVK
                     "
     gene            complement(2803..3168)
                     /locus_tag="PLR93_02340"
     CDS             complement(2803..3168)
                     /locus_tag="PLR93_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH25826.1"
                     /translation="MNAIKFTPDGGQVKVMGRYINGSEPPQVEVSIKDTGIGVDTSMK
                     DRIFEKFGQTGEVLLHSSGKTKFKGGGPGLGLAIARGIIQAHGGRIWVDSPGYNETTY
                     PGSTFTISLPAHKEKERAS"
     gene            complement(3155..4666)
                     /locus_tag="PLR93_02345"
     CDS             complement(3155..4666)
                     /locus_tag="PLR93_02345"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNH25827.1"
                     /translation="MSLRLKVSLPFLILTVVISLIGVYVVTRLVTGSMTERLTNQLLE
                     AGRVVADSFVRQEGQHIKEAQIISYTAGLAEALEAGDKETALKLAEPAFGAGTIENMI
                     LISPDGNEIVHLIRDETRVIQRVTENTGAANSPIVAPFLRSRNPADPPTRALGGNLVN
                     NELYYYTSLPVVVDNKFYGVIVVGTSINTLLPAFKHIALADIVLYGNNGQALATTLGS
                     ADKETLNFLSISNAEYQSVLSAPGQVSGINFKLAGRNYILGRAPLQIGNDRIGVLGVI
                     LASDFVVQFGSSNRTNYAIFFSLVMLLVIAIGYFVSRLIIVPLYSLVNTSRAIASGDL
                     ARRTGIRTDDEIGDLANSFDEMTRRLQERTEELEQKNEILKKMDRTKTNFIQISAHEL
                     RTPLTLIMGYSQMLEQDMKDFPELQKLAQGILEGSERMSDVVDSMLDVSRIDSNALVL
                     RKTSVRMEPIIRKVQKGFRTAFDDRNIKFDTEGLENLILRSSKGFLSSCYECH"
     gene            complement(4829..5269)
                     /locus_tag="PLR93_02350"
     CDS             complement(4829..5269)
                     /locus_tag="PLR93_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25828.1"
                     /translation="MDIIVRRIASEERETWFQMRKGIWPDTPDHYLWFDMDKILASET
                     DAVFLAFVDGTPAGMIETRLRDYGEGCSTSPVGYIEGWFVAPEFRGNEVAGLLIVAAE
                     SWAREKGCTEMASDTWLDNEISIRAHIKLGYFEVERLVHFVKKL"
     gene            complement(5296..5763)
                     /locus_tag="PLR93_02355"
     CDS             complement(5296..5763)
                     /locus_tag="PLR93_02355"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNH25829.1"
                     /translation="MTAFKSFLFFVFAPGMVAGAIPLALLQVGPKLDADFLSYLALLF
                     WVVGVGILIWCFGDFLIKGRGTPAPIDPPKELVVDGLYRYVRNPMYVGVLMVIFGHFL
                     WLGYWILLIYAIVVFTAFSAFVIYYEEPHLRKTFGAAYEDYCRRVPRWLPRFK"
     gene            complement(5763..6041)
                     /locus_tag="PLR93_02360"
     CDS             complement(5763..6041)
                     /locus_tag="PLR93_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25830.1"
                     /translation="MFSFSFLKPSLLKIGIAIALFLLSGWLWSVYVTSRIADVFPTGF
                     PLEYYVSWGPCPLNPIEPCSEFNGWFLLVDVFFWYAFGAWLVNYFKKT"
     gene            complement(6062..6295)
                     /locus_tag="PLR93_02365"
     CDS             complement(6062..6295)
                     /locus_tag="PLR93_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25831.1"
                     /translation="MKNPFGDQQIPGSYQDVKQRIYKKVSAKVNDQIRMLLQDSYEHA
                     LNEENIILSRPERKRLFSQVVAMVMEDLSGNLG"
     gene            complement(6300..>6548)
                     /locus_tag="PLR93_02370"
     CDS             complement(6300..>6548)
                     /locus_tag="PLR93_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25832.1"
                     /translation="ESGVAMEINAHPSRLDLDDAYARRAKDLGIPITINTDAHSEEDY
                     DMLHYGVAIGRRAWLEPKDVINTWTAKKLTDWLKKRGK"
BASE COUNT         1832 a         1554 c         1555 g         1607 t
ORIGIN      
        1 gggaatgtgt gcggtggtgg gacatcgggt tcgggaaaaa gttttaacat cccccactct
       61 gcgccgatct gattcgggaa gacatcatta agcaggtgaa tgatctcatc ccgccgattc
      121 gcgcgagcaa gaagatattg catggattga atcgccgact caaatttgag acggtcgggg
      181 tagagtaatt tgtcgagcca gcgttgaatg aatttacggg ttgggtcaaa taatatggct
      241 gccgccaaaa ggctgatagc cggagccagt ggttttgagg taagagaatc cgcaaaaagg
      301 gaggtgaggc ttgtggtcag tgccaggtac agggtcagga gaagaagcga aactgttcca
      361 tagacaagag tccgcctcaa ggcacggtca atgccaaaga ggtcatgccg caacacggcg
      421 taggcgattc ccaacggtac aaaaagttga aagaccagca gtaggttgta ggagaagata
      481 tccaacccca atgccagggt aagcgggcgc aaccaaagcg gaacttcggc aaggatcata
      541 ctaaacaaaa cgacccgcgc gcgttgggca tcgagaatat tttcggtgtt tggatcctgt
      601 gcaacacgca ggagattttt aaaaaagaaa aggatgataa aagtgaacca gataaaaagc
      661 aaaacaatgg tcaaaatacg ctgccatgca aagacactaa acaacaatgc aacagttaat
      721 gctgccgaaa aaatatagcc tgccacaatt ctcgacattg gcaaatcgga ccggcgtgga
      781 aaccgtacac taacatggag agccattggc aggagaatgg cagcctgggc cagacgcaga
      841 acaagatacc cgtccagaaa ggttatgaga gatcttcccg ggtgaatcgt tggcaggtca
      901 ttttgttcaa agggaacaag caaaaaaaat gcaaaaaaga tcgaaaagca agcaatcagg
      961 gtgtgacttt cctgtgggcg tgcaaaggca ggaataagtc cggtaacaag aataagcaaa
     1021 aacacaacac ccgaaaaggt cgttgctgag cgaattacag tccctcctga tgtacgcatg
     1081 acccatccaa acagagccgc gtttatgaag aatacgacca gaatgggtat aaataatgct
     1141 gcacgtttca ttacagcaat tgtaaaggaa gaaaaagaga ccgaggggtt cggtctcttt
     1201 ttgcaattta tggtgttgct gtctcttgcg gcacagaggt ttcttcaaca ggtgttgcat
     1261 ctatcgtctc atctgttgag atcgattctg ttggaacctg atccgtgttg atctcttcag
     1321 ggctgggagt tgggactgtc tcctcagtgg aggttaccaa gtcttcgatc gcggtaacca
     1381 gttctgtgat gatcgcctca agagaagggg ttgggttggt gtctggaaca gtaacctcat
     1441 tgacggttgg ctgggcgggt aaatcggcaa ccggtcccgg aggcggagta gggatgggaa
     1501 tttctgtaac cacgatcgca tcaacctggg tcacatcgcc cgtaccaaga ttgggagaga
     1561 taataacgat gtaattgtag tatacatctc ctggcgcggc gctgagagcc gtatcaacat
     1621 cgatcaaaat accagtggtg atacctgtgg cagggtcggt atataaacta cccagaggga
     1681 tcactcgatt atcacattct acattggatg cacatgggcc ctggtcaggc aatgtattcg
     1741 tattcgccgt ggtattcgtg tctgcaatat tattgcccca attaaagacc tcatagtagg
     1801 tggcgccatt tgagtcattt gaaataccaa taatgatatt gtccaattca acatgagggg
     1861 tgccgccaaa attcgtcaga tattcataaa aggccagatc ataattacca tcgggagtta
     1921 aggaaacata aacagggttt cctgaaatgc tcaccactgt ataagtccca gtgccacctc
     1981 cagggttatt tgggaggtcg cccaccacgc catctgggcc atcggtttca atgcccacag
     2041 gtggggtgat cggtgttaga ttagtatccg gggtggatgg cgttcccgtg gcagtggcag
     2101 tagccgtcgt tgttgaggta gtggttgttg ttgatgtcgc tgtcgttgtc gaggtagcag
     2161 ttgttgttga tgtcgctgtc gttgtcgagg tggcggttgt tgttgatgtc gccgtcgttg
     2221 tcgaggtggc agttgtcgtt gacgttgctg tcgttgttga cgttgctgtg gtcgttgagg
     2281 tggcagttgt tgtagcagtt actgtaacag tcggagtatc tgtaattgtt gaggtaggcg
     2341 tcaccgtagg agtgtctgtc ggctcctctg tttctgtctc tgttggagtg gttgttacag
     2401 ttctggttga agtagcggtg gcagtggttg ttactgttga agtggctgtt ggggtggtct
     2461 cagttgcggt tgtggtcgcg gtcggagtca catcggttgg ggtaggtgtg acggttggtt
     2521 caaaggttaa gaaaggcgga tattcaatat caccatccgg ggtcaagtag ctatctgccc
     2581 aggccatggg agtaagaatt tggggcagga tcggctcaag cagcgcgaat gggcgcgctg
     2641 aagaggcatc cggatccaga ttcgatccca tgccagcatt gatactccaa aatggcatca
     2701 tacgaattgc catttgtcca acaatgatga tcattaagat cccgagcggt attagaaata
     2761 tcaatatgct caggtcgcga cgttcacgtt cttgaactgt cgtcatgatg ccctctcttt
     2821 ctctttgtgg gcagggagcg aaatggtaaa agtactccca ggatatgtgg tttcattata
     2881 ccccggactg tcgacccaaa ttcgcccgcc atgtgcctgg ataattccgc gtgcaatcgc
     2941 aagccctaaa cccggaccac cgcccttaaa tttggtcttc cccgaagaat gcaaaagcac
     3001 ctccccagtc tgaccaaact tttcgaaaat acgatctttc atactcgtat ccaccccaat
     3061 tcccgtatcc ttgatcgaga cctccacttg cggaggttca gaaccattta tataccggcc
     3121 cataaccttc acctggccgc catccggcgt aaatttaatg gcattcataa caagatgata
     3181 aaaaaccttt tgacgatcgc aatattaaat tctccaaacc ttcagtatcg aatttaatat
     3241 tgcgatcgtc aaatgcagtt ctaaatcctt tttgtacctt gcgaatgatc ggttccattc
     3301 ggacacttgt cttccttaat acaagggcat tgctatcaat acgagacaca tccaacatac
     3361 tatccaccac atcagacata cgctccgaac cctcgagaat gccctgagca agtttttgca
     3421 attcagggaa atccttcata tcctgttcca gcatttgaga atagcccatg atcagggtca
     3481 acggagtgcg taactcatgg gcagaaatct gaatgaaatt cgttttcgtt ctgtccatct
     3541 tcttgagaat ttcattcttc tgttcaagtt cttcagtacg ttcctgcaaa cggcgggtca
     3601 tttcatcgaa cgaatttgcc aggtcaccaa tttcatcatc tgtacgaatg cctgttctac
     3661 gagcaaggtc cccgctcgca atggcgcgag acgtatttac caaagaatac aatggcacaa
     3721 tgatcaaacg tgatacaaaa taaccgatgg cgatcaccaa aagcataacg agcgaaaaga
     3781 agatcgcata gtttgttcga ttacttgacc caaattggac aacaaaatca gaagccagga
     3841 tgactccaag caccccaatc cggtcattcc cgatctgcaa gggggcgcgc cctaaaatat
     3901 aattacggcc tgccaattta aaatttatgc cactgacctg gcctggggca ctcaaaacac
     3961 tttgatattc tgcattagag atcgacaaaa aattcaatgt ctctttatca gccgatccaa
     4021 gtgttgtagc aagcgcctgg ccattatttc catatagaac aatatctgca agtgcaatat
     4081 gcttaaaagc gggcagcaat gtattgattg atgttccaac aacgatcact ccataaaatt
     4141 tgttatcgac aacaacgggg agagatgtgt aataataaag ttcattatta accaaatttc
     4201 cgcccaacgc acgggtaggt ggatctgctg gattcctgct tctaagaaaa ggagccacaa
     4261 tgggagaatt tgcagcacct gtattttccg tgaccctttg tatgacccga gtttcatcgc
     4321 ggattagatg gactatttca tttccatccg gtgaaatcaa aatcatattc tcaatcgtcc
     4381 ccgcaccaaa ggctggttca gccagtttta gagccgtctc tttgtctcct gcttcaaggg
     4441 cttccgccaa gccagccgta tatgaaataa tttgcgcctc ttttatatgc tgaccctcct
     4501 ggcgcacaaa actatcagcg accacccgcc cagcctccag caattgattg gttaaccttt
     4561 ccgtcattga cccggtcacc aggcgcgtga caacatagac cccgatcaat gaaatgacca
     4621 cggtcaatat aagaaaaggt aaagagacct taaggcgaag gctcatgtga aaacctgtac
     4681 aaaaacgcca caggcattat actgggatgc taatagcaat acttcgaaat gcaacataat
     4741 tataatgtca acagggttga aaaatagtga catttttccg cttctctccc cgccaaagca
     4801 tgttcagtcg tccagaccac ttccccacct acaatttttt cacaaaatgc accaacctct
     4861 caacttcaaa ataccccaat ttgatatgcg cacgaatgct aatctcattg tccaaccagg
     4921 tatccgaagc catttctgta cacccctttt ccctggccca actctccgct gctacaatga
     4981 gaagcccggc aacctcattg ccgcgaaatt ccggagcgac aaaccacccc tcaatgtaac
     5041 cgaccgggct ggtggagcaa ccctcgccgt agtcgcggag gcgggtttcg atcatcccgg
     5101 ccggagtccc atccacaaac gccagaaaaa cagcatccgt ttcactggca aggattttgt
     5161 ccatatcgaa ccacagataa tggtccggcg tatcaggcca gattcctttt cgcatttgaa
     5221 accaggtctc acgctcttcg gaggcgatgc gtcttacaat gatatccata aaattcctcg
     5281 aaacgaatac aaccctcact tgaaacgcgg aagccagcgc gggactcttc gacagtaatc
     5341 ttcataggct gcgccaaagg tcttccgaag atgcggctct tcataatata tgacgaaggc
     5401 ggaaaaagcc gtaaatacaa caatcgcata gatcaatagg atccaatatc caagccacaa
     5461 aaaatgaccg aagatcacca tcaatacacc aacatacatc gggttgcgga cataacggta
     5521 taacccgtcg actaccagtt cctttggcgg atcaatcggt gcaggcgtac cgcggccttt
     5581 gataagaaaa tccccaaagc accaaatcaa tatccctacc ccaacaaccc aaaataatag
     5641 agccaaataa gagagaaaat ctgcatccag ttttggaccc acttgcaaaa gcgccagcgg
     5701 aatggctccg gcaaccatac ctggagcaaa aacaaaaaaa agaaaagatt taaaagctgt
     5761 cattaagttt tcttgaaata attgaccagc catgcgccga aagcatacca aaagaaaaca
     5821 tcgaccaaca ggaaccagcc attaaactcc gaacaaggct caattgggtt taatgggcat
     5881 ggcccccatg agacataata ctcaagtggg aaccctgtgg gaaaaacatc ggcaattcga
     5941 gaggtaacat aaacactcca tagccagcca gacagcagga acaaggcaat ggcgattcca
     6001 attttcaaca gggaaggttt caaaaaagaa aaagagaaca ttctgaattc tcctttcaag
     6061 gtcaacccaa attcccggat aggtcttcca ttaccattgc tacgacctgg gaaaaaaggc
     6121 gttttctttc cgggcggctc aatataatat tttcttcgtt taaagcgtgt tcataggagt
     6181 cttgcagcag catccgaatc tgatcattga ctttcgctga tactttttta tagatgcgtt
     6241 gttttacatc ctgataagaa ccggggattt gttgatcgcc aaagggattt ttcatttgat
     6301 tacttccccc tcttcttcaa ccaatccgtc agcttcttcg ccgtccaggt attgatgaca
     6361 tccttcggct ccagccaggc gcggcggcca atggcgactc cgtaatggag catgtcgtaa
     6421 tcttcttcgg agtgcgcatc ggtgttgatg gtgatgggaa tgcccaggtc tttggcgcgg
     6481 cgggcgtagg catcgtcgag gtcgaggcgt gagggatggg cgttgatctc catcgccacg
     6541 ccggattc
//
LOCUS       DAOQFT010000055         6484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5870_length_6484_cov_7.090994, whole genome shotgun sequence.
ACCESSION   DAOQFT010000055 DAOQFT010000000
VERSION     DAOQFT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5870_length_6484_cov_7.090994"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="PLR93_02375"
     CDS             complement(<1..2232)
                     /locus_tag="PLR93_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH25833.1"
                     /translation="MSIGLGVGVFIAGLVIITLALLMLRLVARTHSDPQESSTSPILT
                     KSNSRDAVIVIQPGGRVEYLSAAARPYFNLREDEPFDLERLARHVRPSDDFIDLCVTP
                     GVRRVSINGKPIELSSYEVPGAYPRMLISVRGKDASAASISNNSSESDEILRVATEFG
                     QTIAASLDLDTTVRSILDHVGRLVPSDVLELKLWNEERQILTPFRFQQAASSKVITAS
                     LSRFGGLTDQLSARREPIILNDVESIHEVLSGNELNPIHSYMGIPLMAGGELVGTLEA
                     GQLSSGAFGQHDLNLLHLISGQAAIAIRNARLYEDEQRRRAELMGLANLNQALGAVRD
                     GQDVIAQLVESIAPLFDAEIVGFLLYDENKRTLEGRNPFRGLPAHFVEIYRATILADS
                     PAEAVIARQEPVITTNAMADESWRALGLADIATAASLRDIALIPLLSSGRMLGYLQVG
                     HHARGVVSFNAEELRLMSIVANQSASIIANILLVQQARTRAQRADALRRIASLAASSA
                     TLDEILKYSVLELARLFNADRGAVFLLDEARGMLILNSPSMYGVPPEIGDNFIQIFLD
                     DPDFRHTVSGGKRPFLSGHLSSERRILPAYRPLATALAMESAMVVPLVVRDRSVGELM
                     LGSKKQDHFNSSDLQAVSTAAGQLAAAIESAGLLSQTDNTLRHRVDQLSFITRVNREL
                     ISSLDIRHLLGVLREEAVRISQADCASAVLFELPSSQTEPVIQLAVGCDRVPTVTPPE
                     WIVI"
     gene            complement(2250..3155)
                     /locus_tag="PLR93_02380"
     CDS             complement(2250..3155)
                     /locus_tag="PLR93_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25834.1"
                     /translation="MGTARLLVVEDDVDIANMLKIYFTGLQYDVDVANRGRDALEKTR
                     QVLPHLIVLDIMLPDIDGYEVCRTLRTNTRTSHIPVIFLTQKDERSDKLQGLELGADD
                     YITKPFDIEELKLRVQGAIRRAERESLTDPRSGLPAGRLIEEQLRRIIREKSWALLDL
                     RVNNFEAFKDVYGFVAGDDVMRFAAMLIGEVVDELGTPNDFIGHAGGDNFIIITTNEA
                     APLIRQKIKDRFAEEVQSHYNFIDRQQGFIQAPKAEGGVDKVGFMTFSAGLVSPELQS
                     FADIREITELAAEARRQDTATATGV"
     gene            complement(3224..4312)
                     /locus_tag="PLR93_02385"
     CDS             complement(3224..4312)
                     /locus_tag="PLR93_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25835.1"
                     /translation="MDSFAFIIHPIDPKRDVSRKYPFLGKVLTEKQIDFFSTFFPPVY
                     ISEIEGITSKATGKQIKGWFLACPYTPRRMMQLPERTVYRKIIQTGRAAEELGANILG
                     LGAFTSVVGDAGVTIANSLDIPVTTGDSYTVMMAVQAIREAAKVMDIKIEDATVAVVG
                     ATGSIGRVCAELIAGEAARTLLIARDEKKLEDLRDRLKVQARSELCISTKMDVLKEAQ
                     LILTVTSAIHDVIQPEHLQPGSVVCDVARPRDVSAMVAAVRDDILVIDGGMVDVPGPV
                     NFHFNFGFPEGKAYACMAETIALALEGRFEDYTVGKDITVDRVKEITAIAEKHGFGMS
                     GFRSFEREVTEEQIEAVRRNARRGRAVK"
     gene            complement(4313..5590)
                     /locus_tag="PLR93_02390"
     CDS             complement(4313..5590)
                     /locus_tag="PLR93_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25836.1"
                     /translation="MLKIILHAPNQIMPFCEPARELRIQNTPLWLHQRNLLAPYVTRE
                     MELKQGERLQPVREPAIVYRDNLFFDEAFITEFMTQALKLNRPVRAAFRADDPAFREH
                     ALPLSTSYTPAGSLYLADLWYYPNGPVAGAEPLVIDMQAKEIGYYHVPTYMADQSGDL
                     VFQVPLRCMIAIDSWVHIFIADMVFSQFARGARFEKRLNEDLMFKLKILGKAVYEGRQ
                     VLECSELVKVGKGCVIDPSAVIHGPTIIGDNVTINAGAVIENCVIGSNVNVSQDVQLM
                     LSVVGDGAFLPFRTALFMTTVMENSMVAQNTCLQMCVVGRNTFIGAGSTFTDYNLIPA
                     PLRARDGNGNLSISNRPVLGGAVGHNCRIGSGMIIYPARTIESDVVLAASTERRVIDR
                     DITFDQSDHHKLKLAHLHQTPYHRNRKAEVESW"
     gene            complement(5659..6270)
                     /locus_tag="PLR93_02395"
     CDS             complement(5659..6270)
                     /locus_tag="PLR93_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNH25837.1"
                     /translation="MSETYHYPQKMGRIVLLGMEEVIGKGGMDAVLNFAALNDLQPSA
                     TNERTFSFETVSKLHQSLEQVYGPRGGRGLALRSGRACFKYGIKEYGSLLGLTEMAFR
                     LLPLPTKLHTGARSFADLFNKHTDQQVRVEETESQILWHIERCPLCWERKTEEPVCHL
                     AVGLLQEALYWLSGGKVFSVEETACHARGDEHCIITIQKTPLT"
     gene            complement(6282..>6484)
                     /locus_tag="PLR93_02400"
     CDS             complement(6282..>6484)
                     /locus_tag="PLR93_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH25838.1"
                     /translation="PSLHTIQAPVMGMYGDKDIVVNPNQWKPLLDGVSKARIERFHTA
                     GHFIMLDEPITFNQKLKSFLDE"
BASE COUNT         1741 a         1748 c         1478 g         1517 t
ORIGIN      
        1 gattacaatc cattcaggag gagtcactgt cggaacgcgg tcgcaaccca ctgccaattg
       61 gatgacaggc tcggtttgag aactgggcaa ttcaaagaga actgccgaag cgcaatcggc
      121 ttgggagatg cgaacagctt cttctcttaa aacacctaac aaatgacgaa tatcgagcga
      181 agaaatcaat tcgcgattta cacgtgtaat aaaggaaagt tggtctacac ggtggcgaag
      241 tgtattatct gtttgagata acaagcccgc agactcaatc gcagccgcca gctgacctgc
      301 cgctgttgaa accgcttgta agtccgatga attgaagtga tcctgtttct tgctacccag
      361 catcaactca cccacactgc ggtcgcgaac cactaacgga acgaccatgg cagattccat
      421 ggcaagtgcg gtggcgagtg gacgatatgc gggcagaata cggcgctctg agctgagatg
      481 ccccgaaagg aagggacgct taccgccaga aacggtgtga cgaaaatctg ggtcatccaa
      541 gaatatttgg atgaagttat ccccgatctc aggtggaact ccatacatgg atggagaatt
      601 aagaatcaac ataccacggg cttcatccaa caagaaaacc gcaccgcggt ctgcattgaa
      661 aaggcgcgcc aattccagaa cagaatattt tagaatttca tcaagggtgg cagaagatgc
      721 cgccaaactt gcaatacggc gcaatgcatc ggcacgctga gcacgagtcc gcgcttgctg
      781 cacgagcaga atattcgcga tgatcgaagc agattgattg gcaacgatgc tcatcaaacg
      841 caattcttca gcattgaaag aaaccacccc tcgagcgtgg tgaccaacct gaagatagcc
      901 gagcatgcgt cccgaagaaa gaagcggaat gagagcaata tcgcgcaaac ttgccgcagt
      961 tgcaatatcc gctaagccta acgcacgcca actttcgtct gccatggcat tggttgtgat
     1021 caccggctcc tgacgcgcga tgacagcttc ggcagggcta tcagccaaaa tcgtggcacg
     1081 ataaatttct acaaagtggg caggcaaacc acggaaggga tttcgtcctt ccaaggtacg
     1141 tttattttca tcatatagaa gaaaaccaac aatctccgca tcaaacagcg gagcgatgct
     1201 ttctaccaat tgcgcaatta catcctgccc atcgcggaca gccccaagcg cttgatttaa
     1261 attcgcgagc cccatcaatt cggcgcggcg tctttgctca tcttcataca gccttgcgtt
     1321 gcgaatggca atcgctgcct gccccgagat cagatgcaga agattcaagt catgctgacc
     1381 gaaagcaccg ctacttagct gaccagcttc taacgtgccc accaactccc caccagccat
     1441 gagcggaatg cccatatacg aatggatggg atttaattca ttacctgata aaacttcatg
     1501 aatagactca acatcattaa gaatgatcgg ttctctccgc gccgataact ggtcggtaag
     1561 accgccaaag cgcgaaagcg acgccgtaat cactttgctc gaagccgctt gctggaagcg
     1621 aaatggagtc aggatctggc gttcttcatt ccaaagcttc aattccaaaa catctgaagg
     1681 aaccaaacgc ccaacatggt caagaatcga gcggaccgtt gtatccaagt ctaagctagc
     1741 agcaatggtc tgaccaaact ctgttgccac acgcaagatt tcatccgatt cagatgaatt
     1801 atttgagatg cttgcagcgc tcgcatcttt cccacgcaca gaaatcaaca tgcgcggata
     1861 tgcccccggc acttcataag aagaaagctc aatcggcttc ccattgatag aaacgcgccg
     1921 tactcctgga gtcacacaca gatcaataaa atcatctgaa ggacgcacat gtcgtgcaag
     1981 acgctccaag tcaaatggtt catcttctcg taaattaaag tatggccgtg cagccgcgct
     2041 aagatattcc acccgtcccc cgggttgaat aacaatcacc gcatcgcgcg aattcgattt
     2101 tgtaaggatt ggggaagttg aactttcctg ggggtctgaa tgggtacggg caacaaggcg
     2161 tagcatcaaa agagctagtg taatgatcac cagcccggcg atgaatacac ccacgcccaa
     2221 cccaatagac atttaatact cgctaacgtt tagacaccag tggctgttgc tgtatcttgt
     2281 cttcgcgctt cagccgctag ttctgtaatt tctcggatat cggcaaagga ttgcagttca
     2341 ggcgagacaa gccccgctga aaatgtcatg aaacccactt tatcaactcc gccttctgcc
     2401 ttcggagctt gaataaagcc ctgctggcgg tcaatgaagt tatagtgact ctgaacttcc
     2461 tctgcaaaac ggtctttaat cttctggcgg atcaaaggag ccgcctcatt ggtagtgata
     2521 ataatgaagt tatcgccacc tgcatgacca ataaaatcat tcggcgtacc caactcatcc
     2581 accacttcac cgatcaacat cgcagcaaag cgcatcacat catcgcctgc tacgaaacca
     2641 tagacatctt tgaacgcttc aaaattgtta acccgcagat ccaacaatgc ccaagacttc
     2701 tcacggatga tgcggcgtaa ctgctcttcg atcaaacgcc cagccggcaa gcctgagcgc
     2761 gggtcggtga ggctctcccg ttccgcacgg cggatggcac cttgaacgcg taacttcaat
     2821 tcttcaatat cgaaaggctt ggtgatgtag tcatccgcgc ccagttccaa cccttgcagc
     2881 ttatcgctgc gttcatcttt ttgagtcagg aagatgacag ggatatggct ggtacgcgta
     2941 ttagtacgca gtgtgcggca aacctcatac ccatcaatat cgggcaacat aatgtccaat
     3001 acgatgagat gcggcaaaac ctgccgagtc ttctccagcg catcacgtcc acggttggca
     3061 acatctacat catattgcaa acccgtgaaa tagattttta gcatgttggc aatgtcgaca
     3121 tcgtcttcca ctaccaaaag gcgagcagtt cccatgtaag cctcctgctg attgtcaaat
     3181 tcgtattcga tcaaacagca ttgttccttg catttttatt gttctacttt acagcacgtc
     3241 ctcggcgggc attgcggcga acggcttcga tctgttcctc tgtcacttcg cgttcgaacg
     3301 agcggaaccc gctcattcca aacccgtgtt tctctgcgat cgctgtgatc tccttcacac
     3361 ggtctaccgt aatatcttta ccaaccgtgt aatcttcaaa acgaccttct aacgcgaggg
     3421 ctatcgtctc tgccatacag gcatatgcct tgccctcggg aaacccaaag ttaaagtgga
     3481 aatttacggg acctggcaca tccaccatgc caccgtcaat cactaatata tcatctctca
     3541 ccgcggcaac cattgcagaa acatcgcggg ggcgagctac atcacatacc acactccccg
     3601 gctgcaaatg ctccggctgg atgacatcat ggatcgcgct ggtaaccgtg aggataagct
     3661 gtgcttcttt taaaacatcc atcttggtgc tgatgcacag ctcgcttctt gcctggacct
     3721 tgagcctgtc tcgcagatcc tcaagctttt tctcatcccg ggcgatgagt aaggttcggg
     3781 cggcttcacc agctatcagc tccgcacaga cccgcccgat cgaacccgtg gcgcccacca
     3841 cagcaactgt ggcatcctcg atcttgatat ccatcacctt ggcggcttca cgaatggctt
     3901 gaaccgccat catgaccgtg taagagtctc cagtagtgac tggaatatcg agactgttgg
     3961 caatcgtcac accggcatcc cccaccaccg acgtaaacgc cccaagcccg aggatattgg
     4021 caccgagttc ttcggcggca cgacctgtct ggataatttt acgatagacc gtgcgctcag
     4081 gcaactgcat catacgcctt ggagtatacg gacatgcaag gaaccagcct ttaatctgct
     4141 ttccggttgc ctttgaggta attccctcga tttcagagat ataaaccggg gggaaaaagg
     4201 tggaaaaaaa gtcgatctgc ttctcggtga gaaccttccc taaaaacggg tattttcggc
     4261 taacgtcgcg cttgggatcg atggggtgaa tgatgaaggc gaaactatcc atttaccatg
     4321 actcaacttc tgctttgcgg ttgcgatgat agggtgtttg gtgcaggtga gccagcttca
     4381 gtttgtgatg gtcactctgg tcgaaagtaa tatcgcggtc aatcacacgt cgttcggtag
     4441 aagctgccag cactacatcg gactcgatgg tgcgtgccgg gtagataatc atccccgaac
     4501 cgatacgaca gttgtgcccc actgccccac ccaacaccgg gcggttggaa atgctcaagt
     4561 tgccattccc atctcgggcg cggagcggag cgggaatcaa gttgtaatct gtgaatgtag
     4621 agcctgcgcc gataaacgta ttacgtccca ccacgcacat ttgcaaacaa gtattttgcg
     4681 ccaccatgct attttccatc acagtggtca tgaataaagc tgtacggaat gggagaaatg
     4741 caccgtctcc cacaaccgaa agcatcaact gcacatcttg cgagacatta acattgctac
     4801 cgataacaca attttcaata acagcccccg cattaatggt gacattatct ccaataatcg
     4861 tcggtccgtg aatcacagcg cttgggtcaa tgacacagcc cttccctact tttaccaact
     4921 ctgaacattc caaaacttgt cgaccttcgt aaacggcttt accaagaatc tttagtttga
     4981 acatcaggtc ttcgttcaat ctcttttcaa agcgcgcgcc acgagcaaat tggctaaaca
     5041 ccatgtctgc aataaaaata tgcacccatg aatcaatggc gatcatacaa cgcaatggca
     5101 cctgaaaaac caggtcgccg ctttggtctg ccatgtaggt tggtacgtgg taatacccaa
     5161 tttctttcgc ttgcatgtca atgacaagcg gctctgcacc cgctacagga ccattcgggt
     5221 aataccaaag gtccgcaaga tataagctac ccgccggggt atatgatgta gacaaaggca
     5281 aagcatgttc acggaaagcc gggtcatctg cgcgaaaagc cgctcgaacc gggcggttaa
     5341 gcttcaatgc ctgcgtcatg aactccgtaa taaatgcctc atcgaagaat aagttatcac
     5401 ggtaaacaat ggcaggttca cgtaccggct ggagccgttc accctgcttt aactccattt
     5461 cgcgggtcac ataaggggcg agcaaattcc gctgatgaag ccaaagagga gtattttgaa
     5521 tccgaagttc acgcgccggt tcgcaaaaag gcataatctg gttgggcgca tgaagaataa
     5581 tcttaagcat gggcagaatt ataaatcata gcgtgcctct cggcacgcta tgtaagaaaa
     5641 caaaattgtt atgcaatttt aggttaaggg ggtcttctga atggtaataa tgcagtgttc
     5701 atccccccga gcgtgacaag ctgtctcttc cacgctaaaa accttgcccc cactcagcca
     5761 gtacaacgcc tcttgaagca aacctaccgc caaatggcaa accggttctt cggtctttct
     5821 ctcccagcac agcggacagc gctcaatatg ccaaagaatc tgggactcag tttcttctac
     5881 acgcacctgc tggtcggtat gtttattgaa aaggtctgca aaagaacggg cacctgtatg
     5941 cagtttggtc gggagcggca ataaacggaa cgccatttca gtcagcccaa gtagagagcc
     6001 gtattcttta atcccatact tgaaacaagc acgccccgaa cggagcgcca atccacgccc
     6061 accacgcgga ccataaacct gctccagcga ttgatgcaac ttgctcaccg tttcaaacga
     6121 aaacgtccgc tcattggtag cggacggctg taagtcattc aaggctgcaa agttgagaac
     6181 ggcatccatc ccgcctttgc cgatcacttc ctccatgccg agaagcacaa tccttcccat
     6241 cttttggggg taatgatagg tttcactcat tggttttgta tttactcatc caggaaactt
     6301 tttagttttt gattgaatgt aatcggctca tccaacataa taaagtgacc tgccgtatga
     6361 aagcgctcaa tccgcgcttt agacacccca tccaatagag gcttccattg attagggttc
     6421 acaacgatat ctttgtcacc atacatcccc atcacagggg cttgaatggt atgcaagctt
     6481 gggc
//
LOCUS       DAOQFT010000056         6447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5924_length_6447_cov_5.895964, whole genome shotgun sequence.
ACCESSION   DAOQFT010000056 DAOQFT010000000
VERSION     DAOQFT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_6447_cov_5.895964"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PLR93_02405"
     CDS             <1..777
                     /locus_tag="PLR93_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNH25839.1"
                     /translation="IWDFRILLPLFAVSLTLYLMAQIEWKDVKRAWIYIGILVFFIVG
                     LNGLLSGRGGPSSVTNLPSPTVFEVPIKIPFTDIGWDVPVTVSKMWFALNQMTRMLTM
                     AVLALPIPYTMDPNIYGTAFRQMGASDKVSFTMDLAFRFLPTFARDFAITMDAQRARG
                     YEMEKLQGGIAGRLRRLAPLIIPVVMQSTVTGEEVIDAMDLRAFGTKPRTWLKDLKYA
                     PRDYVLLGLGVGIFLACCILKWGFGIGEFFVPDFFLNLFV"
     gene            complement(821..1651)
                     /locus_tag="PLR93_02410"
     CDS             complement(821..1651)
                     /locus_tag="PLR93_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNH25840.1"
                     /translation="MAEKTYRQTINEWRKERNEGIRKENAWLSLAGLYWLKLGKNQFG
                     SDPKSEILLPERIAANVGHFEYNGKSVSLRANPGQKVTVNDKATDFALLQPDISESPS
                     YIQLEDIQMVVIQRGNRMGVRMWDNKSEKRASFPARTWYDIDEAFRVPATFFAYDRPK
                     MAYFPDLTGEKSEFPVEGYVTFTLDGKEHKLDINKEEDGVLFLRFWDLTANDETYPTG
                     RYLIADVEEDGKIFLDFNRAYNPPCAFTDFATCVFAPEQNHLDVKITAGETYPRDIFK
                     "
     gene            1739..2293
                     /gene="dcd"
                     /locus_tag="PLR93_02415"
     CDS             1739..2293
                     /gene="dcd"
                     /locus_tag="PLR93_02415"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNH25841.1"
                     /translation="MGLKPDHWIRKMALEHGMIEPFVDKQVRQGVISYGVSSYGYDIR
                     VANEFKIFTNVFSATVDPKNFDTNSMVDFVGDVCIVPPNSFALARTVEYFRVPRKVLT
                     VCLGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGLAQVLFFEADE
                     ECETSYADKKGKYQGQQSIVLPKL"
     gene            2332..3582
                     /locus_tag="PLR93_02420"
     CDS             2332..3582
                     /locus_tag="PLR93_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNH25842.1"
                     /translation="MANIRTEESILEELESIQRKMMQTMVRLTSIGNPQVSSINLELT
                     QLVRSLAQLKEDTKARFKRQQQRLEALAEIGGVINSSLDTKHVLDAVMDSLILLMQAK
                     RGFLMLRDETGDFQVQIARDADHQDLTENDFLYSKGIVRRVLRDGQAVLTTSAKDDPR
                     FEQQASVAAYQLLSILCAPLKVKDELIGVIYVDNSAQSGIFGNDDLELITAFSNQAAV
                     AIENAATYQSTLEGWVRALDLRDKETEGHTKRVTQLTEVLARKMEVDNDSLVHIQRGA
                     LLHDIGKMAIPDGILLKPAALTPEERAIIQKHPIYAFEMLSPIHFLHPALDIPYCHHE
                     KWDGTGYPRGLSGEEIPLAARIFAVIDVWDALVSDRPYRRGLNPADVKEKIREDAGKH
                     FDPHVVNAFLDLDDQVLSSNAKIS"
     gene            complement(3633..4475)
                     /locus_tag="PLR93_02425"
     CDS             complement(3633..4475)
                     /locus_tag="PLR93_02425"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNH25843.1"
                     /translation="MPNIYFTLFEIIIYIQFVLCLNHARKQGAANLLKLAFGVLFGVS
                     LELATIRQLHAYEYGQFLLMVLDVPLCIGVAWSCIIYAAMEFSDASSLPYFFRPVLDG
                     LLALNIDLALDAVAIRFGFWDWGQGLTTQYFGVPYANFWAWFWVVFSFSLGYRLLAKN
                     TTWVGTWLSAPMAYFIGLIGVIGTNALIVFVVPTTIRPGVIGLTLASALILVLIRRPS
                     FYLRPVHPVAFWVPFLTHIYILIAGLVSGVILAPVFLLFVGILMLAISFYLHGKTAKE
                     IFPQ"
     gene            complement(4546..>6447)
                     /locus_tag="PLR93_02430"
     CDS             complement(4546..>6447)
                     /locus_tag="PLR93_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNH25844.1"
                     /translation="LFAYQNNTRVVVSTNTINLQDQLIKKDIPDLKAALNLDVRAAVL
                     KGRSNYLCPRRFQYLRSHGPSNATEMRVLAKLIVWQLNNPSGDRNDLNLQGPLEREVW
                     SRLSAEDDNCTTEACLGRMGGTCPFYRAKQAAQNSHILIVNHALLLSDVSTGSKVLPE
                     YDYLIVDEAHHMETAVTNALSFRMTQGDLERMLKELGGSSAGLLGTILTDTHDSLRPS
                     DFGLFQQKAKRATDQAFRVEQLSKEFFSYLGEFIKAQREGQPQSNYSWQMRITPAART
                     LQGWDDLEMVWGQISETMANLLKTLDEIYKSLGELYNDGHDEVEDVMGSLGTLIRRMT
                     EAETAASGLMHNPTNDLIYWIEVNPRGERLSLNAAPLRVGPLVQKHLWYEKASVILAS
                     ATLTTHGEFTYIRNTLDAAEVDTLALGSPFDYESSTLLYVPSDMPEPNAFNYQQILDR
                     SIIATAKATGGRMLVLFTSYAALKKTSQAITGALAREDIFVFEQGEGTSPTALLESFK
                     TTDRAVLLGTRSFWEGVDVPGDSLSVVMITKLPFEVPSDPIISARSELYEDSFNEYYL
                     PEAILKFRQGFGRLIRTASDRGIVAILDRRVLTKQYGRMFLESLPQCTARQGPIANLP
                     KMAGQWLGM"
BASE COUNT         1580 a         1399 c         1765 g         1703 t
ORIGIN      
        1 atctgggatt ttcgcatcct tcttccgctc tttgccgttt cgctgaccct gtatcttatg
       61 gcacagattg aatggaagga tgtaaaacgc gcctggattt atattggtat cttggtcttc
      121 ttcattgtgg gcttgaacgg actcctttca ggacgcggtg gtccttcctc ggtgaccaac
      181 cttccttctc ctactgtttt tgaagttcct atcaaaatcc cttttactga tatcggctgg
      241 gatgtgccgg taacagtttc taaaatgtgg ttcgctttga accagatgac ccgtatgttg
      301 accatggctg tccttgctct cccgattccc tatacgatgg accccaatat ttatgggacg
      361 gctttccgcc agatgggcgc ctcggataag gtctcgttta ctatggacct tgccttccgc
      421 ttcctcccga cctttgcgcg tgattttgcc atcaccatgg atgctcaacg ggcgcgcgga
      481 tatgaaatgg agaaattgca agggggcatt gctggacgtc tgcggcgact tgcacctttg
      541 attatcccgg ttgtgatgca atctacagtg acgggcgaag aagtgatcga tgccatggat
      601 ttgcgagcat ttggcaccaa gccacgcaca tggcttaaag acctgaaata tgcgccgcgt
      661 gattatgtat tgcttggttt gggcgtcggg attttcctcg cctgttgtat tttgaaatgg
      721 ggctttggca taggtgaatt tttcgtgcca gactttttct tgaacttgtt cgtctaacct
      781 gaaaaatcaa aaccgccgct ttaagcggcg gtttttttat ttatttgaaa atatctcgcg
      841 gataggtttc acccgctgta atctttacat ccagatggtt ttgctctggg gcaaagacgc
      901 aggtggcaaa atcggtaaag gcgcaaggtg ggttataggc tctgttgaag tctaagaaaa
      961 ttttgccatc ttcttcaaca tctgcaatga gatagcgccc ggtgggatag gtctcgtcat
     1021 ttgcagtgag atcccagaag cgtaagaaga ggacaccatc ttcttcttta ttgatatcta
     1081 gtttatgttc tttgccatcc agagtgaaag tcacataccc ttcaacgggg aattcggatt
     1141 tttcgcctgt gaggtctgga aaatacgcca ttttggggcg gtcgtaggcg aaaaaggtgg
     1201 cagggacgcg aaaggcttcg tcgatgtcgt accaggtgcg ggcggggaag gaggcgcgtt
     1261 tctcgctttt gttatcccac atgcggacgc ccatgcgatt tccgcgttgg atgacgacca
     1321 tttgaatatc ttccaattgg atatagcttg ggctttcgga gatatcgggt tgaaggaggg
     1381 cgaagtctgt ggctttatcg ttgacggtca ctttttgccc ggggttggcg cgtagtgaga
     1441 cggacttgcc gttgtattca aaatgaccta cattggctgc aatgcgttcg ggaagaagga
     1501 tttcactttt agggtctgag ccgaattggt ttttgccaag ttttagccaa tataaacctg
     1561 ccagtgaaag ccaggcattt tctttgcgaa tgccttcgtt tcgctctttg cgccattcgt
     1621 tgatggtttg acgataggtt ttttctgcca tattttaaaa ttataccaaa agggttttga
     1681 gtgaacgctc tgctttcatt tttgataaaa tgaacttata tcacttaagg agcagagcat
     1741 gggtctgaaa ccagatcatt ggattcgtaa gatggcatta gagcacggga tgattgagcc
     1801 gttcgtagat aagcaggtac ggcaaggggt gatttcatac ggtgtgtctt cgtatgggta
     1861 tgatattcgg gtggcgaatg agttcaagat ctttacgaac gttttctctg ccacagtgga
     1921 ccccaagaat tttgatacca attcaatggt ggattttgta ggcgatgttt gtattgtgcc
     1981 gccaaattcg tttgcgttgg cacgtacggt ggaatatttc cgtgtgccac gcaaggtgtt
     2041 gacggtatgt ttggggaaat cgacctatgc acgttgtggc attattgtga acgtcacccc
     2101 gtttgagcca gaatgggaag ggtatgtgac gcttgaaatt tcgaacacaa cgccattacc
     2161 tgccaaaatt tacgctaatg agggtttggc gcaagtcctt ttctttgaag cggatgaaga
     2221 atgtgaaact tcatatgccg ataaaaaagg aaagtatcag ggtcagcaat ccatcgtgtt
     2281 gccgaaatta taggtttgta aatatccctg agtggatata ggagattctc catggcaaat
     2341 atccgtactg aagaaagcat cctcgaagaa ctcgaatcaa ttcaacgcaa aatgatgcag
     2401 accatggtga gactgaccag tattggaaat ccgcaggtgt cttcaattaa tcttgagttg
     2461 acgcaactgg ttcgtagctt ggcgcaactc aaagaagaca caaaagctcg cttcaagcgc
     2521 caacaacagc gactggaagc cttggctgaa atcggtggcg tgatcaattc atctcttgat
     2581 acgaaacatg tgttggatgc agtgatggat tctctaatcc ttctcatgca agccaagcgt
     2641 gggtttctta tgctgcgcga tgaaactgga gattttcagg tgcagatcgc gcgcgatgca
     2701 gaccatcagg acctaaccga gaatgacttc ttgtacagca aaggtattgt gcgccgtgtg
     2761 ctgagagatg gtcaggcggt gttgacgacg agtgcaaaag atgacccgcg ttttgagcaa
     2821 caagcgagcg tggcggcata tcagcttcta tccattttgt gtgcgccatt gaaggtgaaa
     2881 gatgagttga ttggcgtgat ctatgtggat aacagtgcgc aatctggcat cttcggtaat
     2941 gatgatctgg aattgatcac agcgttttca aatcaggcgg cagtggcaat tgagaatgct
     3001 gcaacatatc agtccacgct ggagggctgg gttcgggcgc tggacttgcg cgataaggaa
     3061 accgaagggc ataccaaacg tgtcactcag ttgactgagg tgcttgcccg caaaatggaa
     3121 gtggataacg atagccttgt ccatattcag cgcggcgccc ttttgcatga tattggcaag
     3181 atggcaattc cagatgggat tttgctcaaa cccgcggctc tgactccgga ggagcgggcg
     3241 attatccaga agcacccgat ctacgccttt gaaatgctca gcccgattca cttcctgcac
     3301 cctgcattgg acattcctta ttgtcaccat gaaaaatggg atggtacggg gtatccgcga
     3361 gggttgagtg gggaagagat tccgctcgcg gcacggattt ttgccgtgat cgatgtgtgg
     3421 gatgcgttgg tgtcagaccg cccataccgc agagggctga acccggcaga tgtgaaagaa
     3481 aaaatccgcg aggatgcagg caagcatttt gacccacacg tggtgaatgc attccttgat
     3541 ttggatgatc aagtgttgag ttctaacgcg aaaatcagtt aataaaaaga ggcagtcaat
     3601 tttcagtgac tgcctctttt tattttaatt atttactggg gaaatatttc tttggctgtt
     3661 ttgccatgta gataaaaaga aattgctagc atgaggatgc caacgaacaa gagaaacacg
     3721 ggagcgagaa tcacgccaga taccagccct gcaatgagaa tgtagatatg ggtaaggaag
     3781 ggaacccaaa atgcaacggg gtggaccggg cgaagataaa agcttgggcg gcgaatcagc
     3841 accaggatta aggcggaggc gagagtcaat ccgatcacac cggggcgaat cgtggtggga
     3901 acgacaaata cgatcagggc atttgtcccg ataactccta ttagcccaat gaagtatgcc
     3961 attggggcag aaagccaggt tccgacccac gttgtatttt ttgcgagtag acgatagccc
     4021 agcgagaagg aaaagacgac ccagaaccac gcccagaagt ttgcataggg tacgccgaag
     4081 tattgtgtgg tgagcccctg accccagtcc cagaacccga agcggatggc taccgcgtcg
     4141 agcgcgaggt caatgttgag ggcgaggaga ccatctaata ccgggcggaa gaagtagggc
     4201 agactactgg cgtctgaaaa ctccatggca gcgtagatga tgcaactcca tgccacgcca
     4261 atgcaaagag gcacatctaa gaccatgagc aagaattggc cgtattcata agcatgcagt
     4321 tgcctaatgg tggcgagttc gagggagact ccaaatagaa cgccaaacgc aagtttgagt
     4381 aaatttgcag cgccctgttt gcgagcgtga ttaaggcaga ggacgaattg aatatagatg
     4441 ataatttcga agagggtgaa gtagatattg ggcatggctt ggaccttgag ttattaaagt
     4501 gacagtcatc tttaagatga ctgtcacgta ttgatatttc ttattttaca ttcccagcca
     4561 ctgacctgcc atctttggta gattggcaat tggtccttgc cgggctgtac attggggcag
     4621 agattccaag aacattctgc catattgttt tgtgagcaca cgacggtcga gaatggcgac
     4681 aatgccgcga tctgatgcgg tgcggatgag gcggccaaat ccctgccgga atttgagaat
     4741 ggcttcaggc aggtaatatt cgttgaagga atcttcgtac agttctgagc gtgccgaaat
     4801 aatggggtct gacggcactt caaagggtaa tttggtgatc atcaccacag agagcgaatc
     4861 accgggcacg tccacgcctt cccagaaaga gcgagttccc aagagcacgg cacggtctgt
     4921 agttttgaaa gactcgagta aggctgtggg cgaagtccct tcgccttgtt caaagacaaa
     4981 aatatcttct cttgccagag cgcctgtaat ggcttgcgag gttttcttca aagcagcata
     5041 ggatgtaaac agcacaagca ttcgtccgcc cgtggctttg gcagtggcga tgatggaacg
     5101 gtcgagaatt tgctggtaat tgaacgcatt tggctcgggc atgtcactag gcacatagag
     5161 caaggtgctg gactcatagt caaaagggga gccgagcgcg agcgtatcca cttcggctgc
     5221 gtcgagtgtg ttgcggatgt aggtaaactc gccatgagtg gtgagagttg ccgaagcgag
     5281 aatcacactg gctttttcgt accagagatg tttttgtact aaaggtccta cccgaagcgg
     5341 ggcggcgttt aaggaaaggc gttctccacg cgggttgact tcgatccagt agatcaagtc
     5401 gttggtgggg ttgtgcatca agccagaagc tgcagtttcc gcttcggtca tgcggcggat
     5461 gagcgtgcca aggctgccca tcacatcttc cacttcatca tgaccatcgt tgtataactc
     5521 gccgagagat ttgtaaatct catccagcgt tttgagcaag tttgccatcg tctcgctaat
     5581 ctgcccccaa accatttcga gatcgtccca gccttgcagg gtccgcgcgg cgggtgtaat
     5641 gcgcatttgc caagagtaat tgctttgcgg ctgtccttca cgctgggctt ttatgaactc
     5701 gccaagatat gaaaagaatt ctttggaaag ttgttccacg cgaaaagctt ggtccgttgc
     5761 acgttttgcc ttttgctgga ataaaccaaa atcggatggg cgcaacgaat catgggtatc
     5821 ggtcagaatg gtgccgagta acccagctga tgagccgccc aattctttca gcatgcgttc
     5881 caagtcacct tgggtcatgc ggaaggaaag cgcattggtc acagccgttt ccatgtgatg
     5941 cgcttcgtcc acgatcaggt agtcatattc gggcaatacc ttgctgccgg ttgaaacatc
     6001 tgaaagaagc aatgcatggt tgacaatgag gatgtgtgaa ttttgcgcgg cttgttttgc
     6061 gcgatagaac gggcaggttc cgcccattct gccgagacag gcttcggtgg tgcagttgtc
     6121 atcttctgca gaaaggcggc tccacacttc acgttcgagt ggtccttgta aattcaagtc
     6181 gtttcggtca ccgctggggt tattcaactg ccagacgatg agttttgcca gcacccgcat
     6241 ttcggtggcg ttgctgggtc catgtgagcg caggtattga aagcggcgcg ggcaaagata
     6301 gttcgagcgc cctttcaata cggcggcacg aacatccaaa ttcaaagcag ctttgagatc
     6361 tggaatatct ttcttgatga gttggtcttg caggttgatc gtgtttgtgg atacaacgac
     6421 acgcgtgttg ttctgatagg cgaatag
//
LOCUS       DAOQFT010000057         6392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_5996_length_6392_cov_7.172795, whole genome shotgun sequence.
ACCESSION   DAOQFT010000057 DAOQFT010000000
VERSION     DAOQFT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5996_length_6392_cov_7.172795"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..909
                     /locus_tag="PLR93_02435"
     CDS             277..909
                     /locus_tag="PLR93_02435"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH25845.1"
                     /translation="MQQRSEETRSKIVFSAVKLFSAKGYNTASVDDICADAGISKGAF
                     YHHFKTKQDLFLALLDGWLETIDNAIEASKDKTVPETFMQITEAFPYIFATANEGLPM
                     FLEFWLQASRDEKIWQASVKPYRRYHKYFTDLIKKGVDEGSFVEVDPDLASRMIISTA
                     MGLLLQSLMDPKGAKWEKVARDSTTMLVASMLKNKNTIQTTNQSNQKRKP"
     gene            906..1766
                     /locus_tag="PLR93_02440"
     CDS             906..1766
                     /locus_tag="PLR93_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNH25846.1"
                     /translation="MKIVTDCAADMSNEELKQLGIVQAPLFIQFPEGEVNSADISADE
                     FYNRLEAMRPQIPTTAQPSSGIFAELYRKLAQIDKNILSVHISSGLSGTINSARDGGE
                     QVKGEANVNFWDTLTLSGGERFQVMAAALADKAGWAMDKVQERLTKIREKTEVIYTLD
                     TLEYLARGGRIGRVQAMAGALLNLKPVIRVDTDGKYSTVAKGRSIGKSMSIIADHLVE
                     KYGNAPVWVTVLHGRFAEKADQLAEEFKQKLNVAKMEVQRISPVLGVHTGPGIVGAAV
                     VPMELMGDLV"
     gene            complement(1824..2951)
                     /locus_tag="PLR93_02445"
     CDS             complement(1824..2951)
                     /locus_tag="PLR93_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH25847.1"
                     /translation="MQTTLDPERQKQAKEYARIRRRLWLVDTLLNAAYAIAWLVFGWS
                     ISLREWLATITTNEWLLVALYVAIFGGIFSVFTLPLGYYSGFILPHRFDQSNQSLKDW
                     VIDRIKGLAIGAPLGLLMLELLYLALRLTGSLWWLYAAGGLLLFNVILSNLAPVLIMP
                     LFNKYVPLGDEHKDLEERLLNLAKRANTKVKGVFKFDMSKRTKAANAALTGMGNTRRI
                     ILGDTLINEFTPDEIETVLAHELGHHVHKDIPFLIISGTLLTTIGLFIASQTLNYSVT
                     FFSFNSVADVAAFPALSLILGAYGLLTQPLENYLSRWRENMADDYALVSTGKHEAFAS
                     AFTRLANQNLGEIDPEKWVVFMFYSHPPLGERIAKARKFTA"
     gene            3010..3618
                     /locus_tag="PLR93_02450"
     CDS             3010..3618
                     /locus_tag="PLR93_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HNH25848.1"
                     /translation="MNFISTLGFAGTIIDLLRAAPQLVRLLRERKSFGVSVDTSGTSC
                     VVSLGWTTYGIITNQIFVALASGAIASFFFIITVAALRLGRSAREFRVTPLWFIVLFA
                     AVTFFGKTGLGLMLSISALISNVPQIRVAYKEENLSGLSLGTWLLTLSGGLIWLIYGI
                     LQKDLTIMVSTIFQSVTSAIIIGLKLFKQRTENTTATRFISD"
     gene            3649..4467
                     /locus_tag="PLR93_02455"
     CDS             3649..4467
                     /locus_tag="PLR93_02455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25849.1"
                     /translation="MTAFPPNAYELIRPLLLNMDIHLIGRSILAKQTPAQIFVDNHEH
                     PKALFAQAGHRYILAGDSERDSFNLGIQKLFTNVIFPREIAAGHEGFALYYDSSTWEK
                     RIDTLLSGKKTIHADREYYVCKKLKHKWQDFLPEGFEIKTVDAALLADSNLKHLETLK
                     EEMTSERPSVEDFLAKSFGVCAIYQGELAGWCLSEYNANGRCEIGIETISDYRKRGLA
                     TALTLAFLEHAFSHGITEIGWHCFKRNEASAKTALKAGFEKVCDYQSFIVLLKE"
     gene            complement(4537..5106)
                     /locus_tag="PLR93_02460"
     CDS             complement(4537..5106)
                     /locus_tag="PLR93_02460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNH25850.1"
                     /translation="MKTALLVIDIQKDYFPGGKFPLVEPLAAAEKAYGLLQCFRENNE
                     KHVHIQHISLKPDATFFVRGTEGSDIHDAVAHFEGEPIVYKHYPNSFRETTLFDMLKG
                     WGIERVVITGMMTHMCVDATVRAAADFGFQVWVAEDACATRDLEHNGTVVPADHVHRA
                     FLAAFKSYGQVMMSEQVIARLAAEKANAR"
     gene            complement(5121..>6392)
                     /locus_tag="PLR93_02465"
     CDS             complement(5121..>6392)
                     /locus_tag="PLR93_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH25851.1"
                     /translation="AAGPLMNMLLAFLCVAAYILTAMLFLPFSKDPQTYIWIQTFQNL
                     FFSLAIVNLILVVFNLIPVYPLDGGNIFHAAMEWLFGKTNADRITLVVGIPFLLVLIV
                     FGVISRDYVLLAFCVLIALSISTLNHSLLRNINLGITYLFKRAGYYYLKGDYERAAQL
                     YTAEIEKQPDDINNYLARAGCYLAMAQRERALADVDRVLKLHPNHFLALELRGEILML
                     NKDYDAAFAHFKQVQHINANWAVPYFDLASLHMERGELQPAIENFNKAVVLQPRMPLF
                     YIIRSLAHFKAGDLASAHHDQDVAVGLSPEEALVMVDVNLIVYEDHWDWAKDYYERIL
                     TKNPRNAFALQGFAEACLVNQKWDEAVTFFSRAIEQNPREARLYVGRGKAYVELEKLQ
                     EAKVDFEKAVASSEKLHLKRQAENFLRTLDI"
BASE COUNT         1840 a         1559 c         1429 g         1564 t
ORIGIN      
        1 gcaggtgcaa tgaccatcgc ccgttggcgt gtgatcggca tcgtccttgg aaccttggta
       61 acaggcgcta ttgttctggg catgttgtac gatttactgg caccattatt cttataatcg
      121 ttcaactcaa catagcggac agaacctttg ggctctgtcc gcttttcttt taaacatgag
      181 ttccctcatc ttgacagtgc aaagtttcag aggtaaaaac aaaccgacca gtcggtttta
      241 ttccttttcc agcacccgac tcgtctcaag gacaagatgc aacaacgcag tgaagaaacc
      301 cgttcgaaga tcgttttctc agccgtcaaa ctgttttcag ccaaaggcta caacaccgcc
      361 agtgtagacg atatctgcgc ggatgctggc attagcaaag gcgcgttcta tcatcacttc
      421 aagaccaagc aagacctttt ccttgccctg cttgatggct ggctggaaac catcgataac
      481 gccatcgaag cctcgaagga caaaactgtc cctgagacct tcatgcagat cacagaagca
      541 ttcccctata tcttcgcaac ggcaaacgaa ggcttgccca tgttccttga gttctggtta
      601 caagccagcc gcgatgaaaa gatatggcaa gcaagcgtga agccttatcg ccgctatcac
      661 aaatacttca ctgacctcat caaaaaaggc gtggacgaag gctcattcgt cgaagtggac
      721 cctgacttag cctcacgcat gatcatttca accgcaatgg gattgttgct acaaagcctc
      781 atggacccca aaggtgcaaa atgggaaaag gtcgcacgtg acagcaccac catgctggtc
      841 gctagcatgt tgaaaaacaa aaatactatt caaacaacga atcaatctaa ccaaaagagg
      901 aaaccatgaa aatcgtaaca gactgcgcag cagatatgtc caatgaggaa ttgaaacagt
      961 taggaattgt acaagccccc ctgtttatcc aattccctga aggcgaagta aactcagcag
     1021 atatttctgc cgatgagttc tacaatcgtt tggaggcaat gcgcccacaa attccgacta
     1081 cagcacagcc atccagcggt atcttcgccg agttgtaccg caaactggca caaatagata
     1141 aaaatattct atcggtgcat atctcttctg gactgagcgg cacgatcaac tcggcacgag
     1201 atggtggcga acaagtgaaa ggcgaagcaa acgtcaactt ttgggatacg ctcaccctct
     1261 ctggtggaga gcgctttcaa gtgatggcag cggcattggc agacaaagcg ggttgggcaa
     1321 tggacaaagt tcaggagcgc ctgaccaaaa tccgcgagaa gaccgaagtg atctacactc
     1381 tcgatacatt ggaatatctc gctcgtggtg gtcgcatcgg acgtgtgcaa gccatggctg
     1441 gagccttact caatctcaag cctgtcatcc gtgtggatac agacggaaaa tatagcacgg
     1501 ttgcaaaggg acgttccatt ggcaaatcca tgtccattat cgcagaccat ctcgttgaga
     1561 aatatggaaa tgctccagtt tgggttacgg ttttgcacgg tcgcttcgct gagaaagcag
     1621 atcaactcgc cgaagaattc aaacaaaaat tgaatgtcgc caaaatggaa gtacagcgca
     1681 tctcgcctgt attgggcgtg catacaggtc ccggcattgt cggtgcggca gtcgtgccga
     1741 tggaattgat gggagatctg gtgtaagaaa agtattggaa gacaagaaat aaataggtca
     1801 cgctttaagc gtgacctata cttttaagcc gtaaactttc tagctttcgc aatccgctcg
     1861 cctaatggcg gatgggagta gaacatgaag accacccatt tctcagggtc gatctcgcca
     1921 aggttttggt ttgccaaacg agtgaaggca gaagcaaagg cttcatgttt gcccgtggac
     1981 actaaggcat aatcatctgc catgttttca cgccagcgag aaagataatt ttcgagaggt
     2041 tgagtcaaca agccatatgc gccaagaatt agagagagtg cagggaaagc agccacatct
     2101 gcgacagaat taaaggagaa aaaggtcaca gaatagttta aggtttgaga ggcaatgaac
     2161 agaccaatgg tcgtaagaag cgtaccggag ataatcaaga agggaatatc tttatggacg
     2221 tgatgcccca actcgtgtgc aagaaccgtc tcgatctcat ccggtgtaaa ttcgttgatg
     2281 agggtatcgc caagaatgat acgacgcgta ttgcccatac cggtcaatgc ggcattggca
     2341 gctttggtac gtttggacat atcgaacttg aagacacctt ttactttggt attggcgcgt
     2401 tttgcaagat tgagcagacg ttcttcaagg tctttatgtt catcaccaag tggaacatat
     2461 ttattgaaca acggcatgat gagcaccggc gcgaggttgg aaagaatgac attaaagagt
     2521 agcaatccac cagcagcata taaccaccag agactcccag tgaggcgcaa tgcaagataa
     2581 agtagttcga gcatcaacaa cccaagcggc gcaccaatgg caagtccctt gatgcggtcg
     2641 ataacccaat ctttgagcga ttgattggat tggtcaaaac gatgtgggag aataaagcca
     2701 ctgtaataac caagcggcag agtaaagaca gagaatatgc cgccaaagat ggcaacatat
     2761 aatgcgacta acaaccattc attggttgtg atcgtggcga gccactcgcg cagagaaatg
     2821 ctccaaccga atacaagcca ggcaatggca taggcggcat tcaatagtgt atctaccagc
     2881 caaaggcggc gacgaatacg tgcatattct tttgcttgtt tttgtcgttc ggggtcaagg
     2941 gtcgtttgca taggcggaat tataatatgc gctcactatc gctagtaaaa cttccctatt
     3001 aggacaaaaa tgaatttcat ctcaactctt ggatttgcag gcaccatcat tgaccttctg
     3061 cgcgccgcgc cgcaattggt acggcttttg cgtgaacgga aatctttcgg cgtctcagtg
     3121 gacacttctg gcacgagctg tgtcgtcagc cttgggtgga ctacttacgg catcatcaca
     3181 aatcaaattt ttgtcgcact tgcatctggc gccatagcca gcttcttttt tattatcaca
     3241 gtggctgcct tgcgccttgg gcgaagcgcg cgtgaatttc gcgtgacccc gctttggttt
     3301 attgtcctct tcgcggcagt aacttttttt ggaaaaaccg gtctggggct aatgctttcg
     3361 atcagcgcat tgatttcgaa cgtcccacaa atacgagtag cgtacaaaga agaaaacctt
     3421 tctgggcttt cgctgggcac atggttgctg actctctcag gtgggttaat ctggctgatc
     3481 tatggaattc tgcaaaaaga cctcacgatt atggtttcca ctatctttca gtcggttacc
     3541 agcgcgatca ttattggttt gaagctcttc aaacaacgaa cagaaaatac caccgccaca
     3601 cggttcattt cagattaact aaaaagacaa aagattggaa actcaacttt gaccgcattc
     3661 cctcccaacg cctacgaact catccgcccg ctgctgctca acatggatat tcacctcatc
     3721 ggtcgttcaa ttctcgccaa acaaacacca gcccagatct ttgtggacaa tcacgaacac
     3781 cccaaagcct tgtttgccca agcaggtcac agatatattc tcgccggtga ctccgaaaga
     3841 gatagcttca atctcggaat tcaaaaatta tttacaaacg tcatttttcc acgcgagatc
     3901 gcagcagggc atgaaggctt tgcgctttac tatgattcat ctacatggga gaaaagaatt
     3961 gatactctgc tttcaggaaa aaagacaatc catgcagatc gcgaatacta tgtctgcaaa
     4021 aagttaaagc acaaatggca agattttcta ccagaagggt ttgaaataaa aactgtagac
     4081 gcagcacttc ttgcagattc caacctaaaa cacctcgaga cgctcaaaga agagatgacc
     4141 tcagaacgcc ccagcgttga agatttcctc gctaaaagtt ttggggtatg tgccatctac
     4201 caaggcgaat tggcaggttg gtgcctctct gaatacaatg ccaatggtcg ctgcgagatc
     4261 ggcatcgaaa ccatcagtga ttatcgcaag cgcgggctcg ctacggcact cactctcgct
     4321 tttctcgaac atgccttctc tcatggcata accgaaatcg gctggcattg tttcaagcgc
     4381 aacgaagcct ctgcgaaaac agcgctcaaa gccggcttcg aaaaagtctg tgattaccag
     4441 tcgtttatcg tcttactcaa agaataggat aaaagaagct gtcacaaaaa acgtgacagc
     4501 ttcttcgctt ctttcttagc cgataattga ctctacttac ctagcattgg ctttctctgc
     4561 agccaatctc gcgatcacct gctccgacat catcacctgc ccatacgact tgaacgccgc
     4621 caaaaacgcc ctatggacat ggtccgcggg gacaacagtg ccattatgtt ccaaatcgcg
     4681 cgtggcacaa gcatcttctg ccacccaaac ctgaaaccca aaatcagccg ccgcacggac
     4741 agttgcatcc acgcacatat gggtcatcat ccctgtgatg actacacgct ctattcccca
     4801 tcccttaagc atatcgaaca aagtcgtctc ccggaacgaa ttggggtaat gtttgtagac
     4861 gataggttcg ccttcaaaat gcgccaccgc atcatgaata tctgagcctt ctgttccacg
     4921 gacaaagaat gtcgcatcgg gcttgagcga aatatgttga atatgaacat gcttttcgtt
     4981 gttttcgcga aaacattgta gcagtccata tgctttttct gcagccgcaa gcggttctac
     5041 gagcgggaat ttcccgccgg ggaaataatc tttttgtata tcaatcacaa gtaaagctgt
     5101 tttcataaca catccttttc ctatatgtcc aaggttctta agaaattttc tgcctgtctt
     5161 ttcaagtgca atttttccga cgatgcaaca gctttttcaa aatctacttt tgcctcttgg
     5221 agtttttcta attctacata tgcttttccc cgccccacat aaagccgtgc ctctcgtgga
     5281 ttctgctcta ttgcacgcga gaaaaaggtc acagcctcat cccatttttg gttgacgagg
     5341 caagcttcgg cgaagccctg aagcgcaaag gcattccgcg gattttttgt caagatacgc
     5401 tcatagtaat ccttcgccca atcccaatgg tcttcataca caatcaagtt gacatccacc
     5461 atcactaagg cttcttctgg cgaaagtccc accgccacat cttggtcatg atgcgcagac
     5521 gcgaggtcgc ccgccttaaa atgcgccagt gaacggatga tatagaacaa aggcatgcgc
     5581 ggctgcaaga caacagcctt attaaaattc tcaattgcag gctgtaattc accacgttcc
     5641 atgtggagtg aagcaaggtc aaagtaagga actgcccaat tcgcgtttat gtgttgcacc
     5701 tgcttgaagt gtgcaaacgc agcatcgtaa tctttattga gcatcagaat ctcaccacgc
     5761 aattcaagtg cgagaaaatg attcggatgt aatttgagca cacggtctac atcggcaagt
     5821 gctcgctcac gctgagccat ggcaagataa cacccagctc ttgccaaata attattaata
     5881 tcgtcaggct gtttttcgat ctcagctgta taaagttgcg cggcacgttc gtaatcaccc
     5941 ttcaaataat aatagccagc tcgcttaaag agataggtaa ttccaagatt gatattcctg
     6001 agcaaggaat gattcaaggt actgatcgaa agtgcgatca gcacacaaaa agcaagcaag
     6061 acataatcac gagagataac gccgaagaca atcaatacca gcaaaaatgg aatacccact
     6121 acaagcgtga tgcggtctgc attggtttta ccaaataacc attccatggc agcgtgaaaa
     6181 atattaccgc catccaacgg gtaaactggg ataaggttga acactaccaa aatcaaattg
     6241 acaattgcaa gcgaaaagaa aaggttctga aaagtttgta tccaaatata agtttgcggg
     6301 tctttcgaaa acggcaaaaa aagcatggct gtaagaatat atgccgccac acacaaaaac
     6361 gccaacagca tattcatcaa aggtccggct gc
//
LOCUS       DAOQFT010000058         6391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6001_length_6391_cov_6.017677, whole genome shotgun sequence.
ACCESSION   DAOQFT010000058 DAOQFT010000000
VERSION     DAOQFT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6001_length_6391_cov_6.017677"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLR93_02470"
     CDS             complement(<1..375)
                     /locus_tag="PLR93_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25852.1"
                     /translation="MNFGSHRPYRRGTNLWLLTLFLAVSCAEPAPASTPDPTLVPLQV
                     NYQPTPTPFQPQPGTSPDPYLAISTPQDAVPTYTPVPTKYVPPQDVSVPVDVVPAAQP
                     GVTLYNPLTGLPVSDPTSCNIVH"
     gene            445..1989
                     /locus_tag="PLR93_02475"
     CDS             445..1989
                     /locus_tag="PLR93_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25853.1"
                     /translation="MLILVTCLLLFITALVLVILRATQPNARYAWLVAAGGATLAFVS
                     VFVWLAQIPFQLTLPVWKPESLFSTPILFSADGVSWALAISIAALTLAVIFTVVTRSV
                     VFNSLSWAGILVLGGMGILAVTANNPLTLLLMWALLDMTELGTQLSSVSGEKNNERVV
                     ISFATRMIGIGLLLWAYIESFTGGGMVVFQTMPPDTGVYLVIAAGLRLGVLPLHLPYA
                     ADSTLRRGFGTALRLIGAASTLSILGHIQILPTNLTPILMILTSVAAIYGGWTWLRAP
                     DELNGRPYWMIGMASLAILSALSGNATGAIAWGSALVLVGGALFLFSIQDIRLSRSLL
                     IGGWALSSLPLSLTASTWASSFNISTIFSLAAQVLLTAGYIRHALRRNGTETLDDQPG
                     WAKVVYPAGILLLLVTQILLGFIGWDGAFKFGNLIFSLLASILALGLVGGSRRFRIFN
                     PVRAHWVTTAGSGVNGLYQWLWSLYRGLAQVAQAITNALEGEGGIMWTLLFLILFISI
                     IVQGIQ"
     gene            1990..2613
                     /locus_tag="PLR93_02480"
     CDS             1990..2613
                     /locus_tag="PLR93_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25854.1"
                     /translation="MVSDIVSWISVALTLVTATTILVNRDWRVSLGALALQYLAAFWL
                     VTRHLPFAMGSVKLITGWMVVAILGMTRLSLTNLDQNEQDAFYPRGAAFRITLMGIVT
                     LVALGSTPRIEAAIPGLGMPVIAGGLLLIGAGVAHLGVTNDLLRVALGLLTVLTGFEI
                     IYAAVESAILVTGLLAVVNLGLGVMGSYLLMAGSIPLISPEEEEELL"
     gene            2610..4007
                     /locus_tag="PLR93_02485"
     CDS             2610..4007
                     /locus_tag="PLR93_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNH25855.1"
                     /translation="MSAPILWIAVPFVLGVLILFVLSERYAAYVGGTISTILAAIAFL
                     IPIDTALLLGNVSLKISPAIEFFGRSFELSTADGRLLTLIYGLAALWFFGTEASGTAH
                     RFVSLGLMITALLTASIAVEPFLYAALLLEMAAMLVVPLLVPLYQRPGRGVTRFIIYQ
                     TLAMPFILFSGWMLAGVEASPGDLGTIVQSGVMVTMGFAFLFAVFPLYNWIPQLIEET
                     NPYAVGFLLWALPTFTTIFALGFLDRYTWLRNTPQISNAILFSGVIMTATGGIFGALQ
                     RHLGRIFGYAAIAETGLLLIALGLRSSEVVNIAFLILIPRGLELSVWALALSIIKRKA
                     YPLKFSDVQGLARKYPVATSALIIAHLSVAGFPLLAGFPSRLALWQELSLQSLSASLW
                     VFLGLLGLLVASVRTLATLVMASDDAPWELNETWVQTFMLGIGVLGIFLLGLFPQILQ
                     PFLSSLPALFEHLIQ"
     gene            4051..5241
                     /locus_tag="PLR93_02490"
     CDS             4051..5241
                     /locus_tag="PLR93_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25856.1"
                     /translation="MEFEQIVKRLEFLDKQQRETKETLTSLKESLASFETTVNAVSKQ
                     IKTLSKQVTDITPAAKRVDQFETMVGKQRNELVKMIEDVDKAHTRSERDTTKQFQSDI
                     NELSKTLTQLKVSTDLTEIKKQLKERGNEIQRALANIADFKTVVEDTKRSNEDVKLSI
                     KAADEARKNDLKRIADLQGELTALRKRIDENREKSTIQGDTIRNIETRITELLSSELE
                     RKQAQAVFLDQQRVAQVDRERAWKDWQDKFVSFQKEAETLDQQIQRLDETLRNAKKAQ
                     DTYMELNSKLERRINEVTEMQRLAEERLRQEWISFKADDQKRWTGYSLSSEESFRDIR
                     KEVSKYEGSISTLNEVSQVLQDQLHQTTDASEKQLQEMMNVVHEWMTSYQRIMGHGKK
                     PVKK"
     gene            5320..>6391
                     /gene="selB"
                     /locus_tag="PLR93_02495"
     CDS             5320..>6391
                     /gene="selB"
                     /locus_tag="PLR93_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNH25857.1"
                     /translation="MRVIGTAGHVDHGKSTLIETLTGTHPDRLKEEKAREMTIELGFG
                     WLTLPNGEELGIVDVPGHRDFIENMLSGIGGIDAVLLVIAADEGIMPQTKEHLAILDL
                     LQIPAGLIVLTKTDLASDSGWLDLVEADIRHAVSETVLKEAPILRVSARAGTGLKELT
                     HALEALLLEKPARPDLNRPRLPIDRVFTMSGFGTVVTGTLRDGHLSVGDEVEILPSGL
                     QGRIRGLQTHKKKEESAVPGSRTAVNVSGVDVEQIRRGDVLAHPGQYQSTRRIDVRFK
                     ALPDISKPLTHGDEVKFFVGASETVAIARLLGTEELKAGEEGWLQLELRDPIVALRGD
                     RYILRRPSPGETLGGGAVVDHLP"
BASE COUNT         1530 a         1511 c         1593 g         1757 t
ORIGIN      
        1 gtggacgatg ttgcaggaag tagggtctga tactgggagc ccggtcaacg ggttgtagag
       61 ggtcacaccg ggctgtgcag caggcacgac atctacaggg acggatacat cttgaggcgg
      121 aacatatttg gttgggacag gtgtatatgt agggacggcg tcttgcgggg tgctgatggc
      181 aagataaggg tctggagatg tgcccggctg aggttgaaaa ggtgtcggcg taggttgata
      241 attaacttga agaggaacaa gcgtcgggtc gggggttgag gcgggggcag gctctgcaca
      301 agaaacggcg aggaagaggg tcaaaagcca gagatttgtt ccacgccgat atggtcggtg
      361 acttccaaaa ttcatggcag ggattgtacc aaaccacaag gtatcagcgc gacaatcata
      421 atactgttac aattatgttt tcccatgttg atcttggtta cctgtctttt gctttttatt
      481 acagcacttg tgctggtgat tttacgcgct acccaaccca atgcacgcta tgcctggttg
      541 gtggcagccg gtggtgccac acttgcgttc gtgagtgtat ttgtatggct tgctcaaatt
      601 ccctttcaac taacattgcc ggtctggaaa cctgagagtt tattttctac acccattctt
      661 tttagtgctg atggggtctc ttgggcgtta gcgatcagca tcgcggcgtt gactcttgcg
      721 gtgatcttca cggtcgtgac acgctcagtc gtcttcaact cgctttcatg ggctgggatt
      781 cttgtgctag gtggcatggg tattttggca gtaaccgcca ataacccact gacgttactc
      841 ttaatgtggg ctctcctcga catgacagag ttaggcacac aattaagctc agtgagcggc
      901 gagaaaaata atgaacgtgt cgtgatctcg ttcgcaacac gtatgatcgg tatcggtcta
      961 ttattgtggg catatattga gagttttacg ggcggcggaa tggttgtttt tcaaacaatg
     1021 ccacctgata cgggtgttta tctggtcatt gcggcggggt taaggcttgg tgtattgcca
     1081 ttacacctgc cctacgctgc tgattcaact ttacgccgcg ggtttggaac cgccttacgt
     1141 ttgatcggcg cagcatctac ccttagtatt ctcgggcata ttcaaatcct tccaaccaac
     1201 ttgaccccga tattgatgat ccttacttcg gtggctgcca tttatggtgg ttggacttgg
     1261 ctacgcgcgc ccgatgaatt aaacggtcgc ccatattgga tgatcggcat ggcttcgctt
     1321 gcgattctct cagcgttaag cgggaatgcc acaggtgcaa ttgcatgggg cagtgcgctt
     1381 gtgcttgtag gcggcgcgct ctttcttttt tccattcaag atatacgtct aagtcgttcc
     1441 ttactcattg gcggatgggc gctttcctct ctgcctctct cgctcacagc cagcacatgg
     1501 gcgagcagtt ttaacatctc gactattttt tcattagcgg cacaggtatt acttaccgct
     1561 gggtatattc ggcatgcctt gcgcaggaat ggcaccgaga ctttggacga tcaacccggt
     1621 tgggcaaagg tcgtttaccc agctggcatt ctcctgcttc ttgtgactca aattctgctt
     1681 ggatttatcg gctgggatgg tgctttcaaa ttcggaaatt tgatcttctc cttgctcgcc
     1741 tccatccttg ctttgggctt ggtcggtgga agcagacggt ttcgcatctt caacccggtc
     1801 cgtgcgcatt gggtcactac tgctggctca ggggtcaatg gactttatca atggctctgg
     1861 tcgctctatc gtgggcttgc ccaagtagca caagccatta ccaatgccct cgaaggggaa
     1921 ggcggcatca tgtggacgtt gctcttctta attctattta tctcaatcat cgtacagggc
     1981 attcaataaa tggtctctga tattgtttct tggatatcgg tcgcattaac ccttgtcaca
     2041 gccacaacga ttctcgtgaa tcgtgattgg cgtgtcagct tgggtgcgct tgctcttcaa
     2101 tatctcgccg cattctggtt agtgactcgt cacttgcctt tcgcgatggg ttcggtcaaa
     2161 ttgattaccg gctggatggt ggtagctatt ctcggcatga cgcgtctcag cttgaccaat
     2221 cttgatcaaa atgaacaaga tgctttttat ccgcgcggtg cagcgtttcg tatcacgttg
     2281 atgggaattg tcacgctcgt agcattagga tccacaccac gcattgaagc cgccattccg
     2341 gggcttggta tgcctgtcat cgcaggcgga ttactactga tcggtgcagg ggtggcgcac
     2401 cttggagtca ctaacgatct attacgtgtg gcgctcggtc tactgactgt gctcactggg
     2461 tttgagatta tttatgcagc cgtggaaagt gccattcttg ttacgggtct tttggcagtt
     2521 gtgaatttag ggttgggcgt gatgggctcc tatttattga tggcaggttc tattccactt
     2581 atttcccccg aagaagagga agaactgcta tgagtgcccc cattctatgg attgccgttc
     2641 cttttgtgct cggtgtatta atcctattcg tattaagcga acgctatgct gcatatgtcg
     2701 gtgggacgat atcaactatt ctggcagcga ttgccttttt aattccaatt gataccgccc
     2761 ttctcttggg aaatgtctcc cttaagattt ctccggctat tgagtttttt ggtcgcagtt
     2821 ttgagctctc cacagcagat ggacggttat taacgctgat ctatggcttg gctgctctct
     2881 ggtttttcgg caccgaagct tctggtacgg cacatcgttt tgtctctttg ggattaatga
     2941 tcactgcatt gctaactgct tctattgcag tagagccatt cttgtatgcc gcactcttgt
     3001 tggagatggc agccatgtta gtagtaccac tgctcgttcc gctgtaccag cgaccaggtc
     3061 gtggtgtgac ccgcttcatt atttaccaga cgcttgccat gccgttcatc ctcttttctg
     3121 gctggatgct tgcaggcgtt gaagcgagtc caggagactt ggggacgatt gttcaatctg
     3181 gtgtgatggt gacgatgggc ttcgcctttc tctttgccgt ctttccactc tacaattgga
     3241 ttccacaact gatagaagaa accaatccgt atgcagttgg ttttctactt tgggcgttgc
     3301 ctacatttac aaccatcttt gctttaggct ttttagaccg ctacacgtgg cttcgcaata
     3361 caccacaaat ttcaaacgct attcttttct caggtgtcat catgaccgct actggcggca
     3421 tcttcggcgc gttgcaacgt catctcgggc gcatctttgg gtatgccgct attgcagaga
     3481 cagggctatt attgattgca ctcggcttac gctcttctga ggttgtcaat atagccttcc
     3541 tcatcctcat ccctcgcgga cttgagttaa gcgtttgggc attggcacta tccatcatca
     3601 aacgaaaagc ctatccgctt aagttcagtg acgttcaagg attagcgcga aagtatccag
     3661 tagcaacgag cgcattgata attgcacatc tttctgtggc ggggtttccg cttttggcag
     3721 gctttccctc gcggttggcg ttatggcaag agctatcgct tcaatcgcta agtgcttcat
     3781 tatgggtctt tcttggctta ttaggattgt tggttgcctc ggtccgcaca ttggcaacac
     3841 tcgttatggc aagcgacgat gcgccgtggg aactaaatga aacttgggtt cagaccttta
     3901 tgctaggtat cggtgttctt ggtattttcc tgctgggact ctttccccag atcctgcaac
     3961 ctttcctttc tagtttgcct gccttgttcg aacaccttat tcaataatcc cgttttgcaa
     4021 aagggtataa tttaacgaaa cggagtcctc atggaatttg aacagatcgt aaaaagactt
     4081 gaatttttag ataaacaaca acgcgagact aaggaaaccc ttacctcgtt aaaagaaagc
     4141 cttgcctcct ttgaaactac agtaaatgcc gtttcaaagc agattaaaac cctgagcaag
     4201 caggtcacag acattacccc tgccgccaag cgtgtagatc aatttgagac catggttggt
     4261 aaacagcgta atgagctggt caaaatgatc gaagatgtgg acaaagccca cactcgttca
     4321 gagcgcgata ccacaaaaca gtttcaatct gacataaacg agctcagcaa aacattgact
     4381 cagctaaagg tcagcaccga tctcacagaa atcaagaaac agctcaaaga gcgcggtaat
     4441 gaaattcaac gcgccttggc aaatattgca gacttcaaaa cagttgtaga agatacaaag
     4501 cgctcgaatg aagatgtgaa gttatccatc aaagcggcag atgaagcccg caaaaatgac
     4561 ctcaaacgca tcgcggacct gcaaggtgag ctcactgcat tacgcaaacg tatagatgaa
     4621 aatcgcgaaa agtccacgat tcaaggcgat accattcgca acattgaaac gcgcattacc
     4681 gaattacttt cttcagaact ggaacgcaag caagcccaag ctgtgttctt agatcaacaa
     4741 cgcgtagccc aagtggaccg tgaacgtgcc tggaaagatt ggcaggacaa attcgtttcc
     4801 ttccaaaaag aagccgaaac tttggatcaa caaattcaac gtttagatga aaccttgcgc
     4861 aatgccaaaa aagcgcagga tacgtacatg gaactcaact ccaagttaga acgccgcatc
     4921 aacgaagtga ccgaaatgca gcgtctcgcc gaagaacgtc tgcgtcagga atggatcagc
     4981 ttcaaagccg atgaccaaaa gcgttggact gggtacagct tatcttccga agagtccttc
     5041 cgcgacattc gcaaggaagt gagcaaatac gaaggcagta tttcaactct caatgaagta
     5101 tcgcaagtgc ttcaagatca actacaccaa accaccgatg cttctgaaaa acaattacaa
     5161 gagatgatga acgtcgttca cgaatggatg acttcatacc aacgcattat ggggcatggt
     5221 aaaaagccag tcaagaaata aaagagcttt ccaaagtgga ggtcgcaaga tttccacttt
     5281 tttattttcg ttaaatcaaa ttgtggttaa aattaaatta tgagagtcat aggcacagca
     5341 gggcacgttg atcacggaaa gtccacgctc atcgaaacgt tgacgggcac gcatccagac
     5401 cgcctcaaag aagagaaagc ccgcgaaatg accatcgagc ttggctttgg ctggcttact
     5461 ctgccaaatg gggaagagct cggcattgtg gatgtacccg gtcatcgtga cttcattgaa
     5521 aacatgctct ccggcattgg cggcatcgat gcggttctgc ttgtcatcgc tgcagatgaa
     5581 ggcatcatgc cgcagacgaa agaacacctt gcaattttag acttattgca gattcccgct
     5641 gggttgatcg tcttgaccaa aaccgacctc gcctcagact caggttggct tgacctcgtt
     5701 gaagcagaca ttcgccacgc tgtaagtgag accgttctca aagaagctcc tattttgcgc
     5761 gtctctgctc gtgcagggac aggtttaaaa gaactcactc acgcccttga agccttgtta
     5821 ttagaaaaac ccgcccgccc agacctgaac cgtccacgtt tgccaattga ccgtgtcttc
     5881 accatgagcg ggtttggtac agtcgtcacc ggcacattga gagatgggca tctttctgtt
     5941 ggcgatgaag tagaaattct tcccagtggg ttgcaaggac gtattcgcgg gttacaaacg
     6001 cataagaaaa aagaagaaag cgctgtacca ggttcacgaa ccgctgtgaa tgtttctggt
     6061 gtagatgtag aacagattcg ccgaggagat gttcttgccc atccaggtca ataccagtca
     6121 acgcgccgaa ttgatgtacg ctttaaggcg cttcctgata ttagcaagcc acttactcat
     6181 ggagatgaag ttaaattttt tgtgggggca agtgaaaccg ttgcaatagc ccgtttatta
     6241 ggcacagaag aactcaaggc gggtgaagag ggttggctac aacttgaatt acgagacccc
     6301 attgttgctt tgcgcggtga ccgatacatc cttcgcagac cctcacccgg tgaaacactt
     6361 ggcggtggcg ccgtagttga ccatctaccc a
//
LOCUS       DAOQFT010000059         6376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6027_length_6376_cov_5.626325, whole genome shotgun sequence.
ACCESSION   DAOQFT010000059 DAOQFT010000000
VERSION     DAOQFT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6027_length_6376_cov_5.626325"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLR93_02500"
     CDS             complement(<1..773)
                     /locus_tag="PLR93_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNH25858.1"
                     /translation="MLSVTEARERILSHFKINSTETVSLIDAANRILGIDILADGDYP
                     PFDNSAVDGFALRAEDTSASPVTLNVVADIPAGSTPKVTLQAGQAARIMTGAQMPLGA
                     NCVIMVEDTDFNVKSAGTAAPKTVTFSKQMKSGENVRLHGTDIHAGDVVLTKGRTLKP
                     QDLGLLATLGHAHVTVHKKARVALLSSGDELLDVSAPLEPGKIRDSNSYALGAAVSSF
                     GAEVIRLGVARDTRESVTQLLDQAANENADLILSSAGVSV"
     gene            complement(844..>1495)
                     /locus_tag="PLR93_02505"
     CDS             complement(844..>1495)
                     /locus_tag="PLR93_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNH25859.1"
                     /translation="TLERELQRLGIPEVNFEKLARDLGFKIPDEMFIGIGTGDLSMSK
                     VIKQFAEDEEIKDILEANTPAAPQKPTDAVEVLGLKGMLASMAKCCNPMPGDQIVGYI
                     TRGRGATIHRLDCPNILQLQDRERLLKVGWGHVEQTFPVPVKIKAYDRQGLMSDISNL
                     LSDENININNVSVNVNRSIADLRLIIEVKDLTQLSRILTRIENLPNVMEAVRNNPG"
     assembly_gap    1496..1595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1596..3212)
                     /locus_tag="PLR93_02510"
     CDS             complement(<1596..3212)
                     /locus_tag="PLR93_02510"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNH25860.1"
                     /translation="MKSAVVEIPIEKLMEQLPESYTVADRELVMRAYTFAEEAHREQK
                     RNSGEPYINHCIAVASILTELKVPADVIAAGLLHDTVEDTPVTLADIRREFGDVVRLL
                     VDGVTKLSHLPRVSRGGEKAENAESEEEPPVQPALLGRKEDMVPETLRKTFLAMGEDV
                     RVVLIKLADRLHNMRTLGHMPEHKRRRIAAETLDIFAPLANRLGIWQLKWELEDLSFR
                     YVQPEKYKEIVESLAERRPDRERQLEEIKGNLIKLLEKNNIKAEVSGRPKHIYSIFKK
                     MQKKDKSFGMLYDVRAVRLIVPDVPSCYAALGVIHTTWRPVPGEFDDYIAAPKDNHYQ
                     SLHTAVMYDDKRPLEIQIRTQEMHMNAEYGIAAHWRYKEGGKHDKQFEERIKDLRKMM
                     EWGGEVNDATEFVESMRSDVFRDRVYIFTPRGDAIDLPAGSTPIDFAYTVHTDIGHRC
                     RGARVNGKLVPLDTELKTGDQVEILTAKRGGPSRDWLNNSLGLVKTARAKSKIRLWFK
                     KLEDEQNLATGRTTLERELQRLGIPEVNFEK"
     gene            complement(3336..3788)
                     /locus_tag="PLR93_02515"
     CDS             complement(3336..3788)
                     /locus_tag="PLR93_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH25861.1"
                     /translation="MISTEILSQFSLFDGLPESLLKEIAGISNEVSVKKDDFVFREGE
                     KADKLYFLLKGSIVLRVKLTSRPESITVSFVSSPYRSFGWSGVVAPHHYTSSAEADED
                     SSLLIIPAEPFMTLLEKNPEHGFTVMKRIAEVIADRLRNSHQALLKTI"
     gene            3902..4378
                     /locus_tag="PLR93_02520"
     CDS             3902..4378
                     /locus_tag="PLR93_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HNH25862.1"
                     /translation="MSVTNNVTRFLDSRKIKYIAYELPAEKLGAVEAAQLLNVPQEQV
                     FKSIVTKREKGKPVLTVIPGPRVVDLKLLAGFLGEKKMHLPTEREAEQLTGLQAGGIS
                     PLALINKGFQVVIDASAQNFETIYISGGQRGLNIQLGAKDLAGLVNAKFGDVSREG"
     gene            complement(4428..4889)
                     /locus_tag="PLR93_02525"
     CDS             complement(4428..4889)
                     /locus_tag="PLR93_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25863.1"
                     /translation="MRRLVITLFLFLFLVSACGAPSPDDPVSNDTVTVHTPAAQETSN
                     YIPSPADGSLTRGEVSLEAKELASLESYPVQFMLQLKGELPTPCHQLRIATNPPDKDH
                     KVLVDVYSVVNPAEICIQVLVPFEIAFPLGSYPEGKYSLWVNGEMVAEFQA"
     gene            complement(4936..5391)
                     /locus_tag="PLR93_02530"
     CDS             complement(4936..5391)
                     /locus_tag="PLR93_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25864.1"
                     /translation="MRYLNFFLIAVLLSACSTVPTAEPTSTAVPPYRITPDENPYEPK
                     LEDLGRQIDEVTITSVNLAELYDYSPPRTAIGLIGYMPSVCDELRVEISPPDENFNVF
                     IQVYSLVNPDVQCDNVFQQFEARILLGTYSPGRYYVWVNDAPVGDFVSY"
     gene            complement(5481..6158)
                     /locus_tag="PLR93_02535"
     CDS             complement(5481..6158)
                     /locus_tag="PLR93_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25865.1"
                     /translation="MFNLFKKKEPKQSDPFLDMRTQFMGNVNFNEWPKGDSDAQPWSL
                     FVEARKAYSANNHAEAEKYLRQVTETPDLEPRHYLQAWLFLRHFLKVQPPAEIAKKVY
                     GVMVEVGTPTGVMAVVGYTDHKARTLHSSGGGTVWERPNASMDDKVDALIKAGENAVQ
                     AIPLVVVDIVPAPPKQADHVLICIATPSGIYHGLGTGDFMWKDPNAGPIVNTGGILLQ
                     ALEALKK"
BASE COUNT         1552 a         1490 c         1539 g         1695 t
ORIGIN      
        1 acactcactc cggctgagga gaggataagg tccgcgtttt catttgcggc ttgatccaaa
       61 agctgggtca cagattcgcg ggtatctctt gcgacaccca gccgaatgac ttctgcgccg
      121 aagctgctga ctgcggcacc caaggcatat gaattagagt cacggatctt gcctggctcc
      181 agcggagcgc tcacatccag caactcatct ccagaagaaa gcaatgccac ccgcgctttt
      241 ttgtggactg ttacatgtgc gtgcccaagc gtggcgagca gccctaagtc ttgtggcttg
      301 agagtgcgac cttttgtcag cacaacatct ccagcgtgga tgtctgtgcc gtgtaggcga
      361 acgttctctc ccgatttcat ctgtttggaa aaggtgactg tttttggcgc ggcggttcct
      421 gcacttttga cgttgaaatc tgtgtcttcc accataatga cacagtttgc accgagtggc
      481 atttgggctc cggtcatgat gcgtgcggct tgacctgctt gcagagtcac tttcggcgtg
      541 gatccagctg gaatgtcggc aaccacattc aaggtcacgg gagaggcgga ggtgtcttcc
      601 gcgcgcagag caaatccatc cactgccgaa ttgtcgaacg gcggatagtc tccatctgca
      661 agaatgtcta ttcccaagat gcgatttgcc gcatctataa gagaaacggt ttctgttgag
      721 ttgatcttga agtgggaaag gatgcgctcg cgcgcttcag taacggaaag catatcgaag
      781 cgccgcgatt atacccccat ctctttttca caccttattt cctgatgaga aaagcgagaa
      841 tggttagcct gggttattgc gtacggcttc catgacattc ggtaggtttt caatgcgcgt
      901 cagaatacga ctcagttgtg tgaggtcttt gacttcgatg atgagacgta aatcggcaat
      961 gctgcggttg acattgacgg agacgttgtt gatgttgatg ttttcatcag aaagcaaatt
     1021 ggaaatatcg ctcatcagac cctgacggtc atatgccttg atcttgactg gtacagggaa
     1081 ggtttgctca acatgccccc agccgacttt gagcaagcgt tcgcggtctt gtaattgaag
     1141 aatattcggg caatcaaggc ggtgaatggt tgcgccacgt ccgcgagtga tgtagcccac
     1201 gatctggtca ccgggcatgg ggttacaaca tttcgccata gacgccagca tgcctttcaa
     1261 gccgagaact tcaacggcat ctgtgggctt ttgtggagcg gcaggcgtgt tggcttcaag
     1321 aatatctttg atttcttcat cttcagcaaa ctgtttgatg accttactca tcgaaagatc
     1381 acctgtacca atcccaataa acatttcatc tggaattttg aagccaagat cgcgagctaa
     1441 cttttcaaaa ttgacttctg gaatgccaag cctttgtaac tcgcgttcaa gcgtgnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctttt caaaattgac ttctggaatg
     1621 ccaagccttt gtaactcgcg ttcaagcgtg gtacgacccg tggcaagatt ttgctcatct
     1681 tctagcttct taaaccacaa gcggatcttg gatttagcgc gggcggtttt taccaaaccc
     1741 aaactgttgt tcaaccaatc gcggctggga cctccgcgtt tggcggtgag aatttctact
     1801 tgatcgccgg ttttcagctc tgtatccaat ggcacaagtt tgccattcac gcgagcgcca
     1861 cggcagcgat gaccaatatc ggtatggact gtgtaggcaa aatcgatcgg ggtagaacca
     1921 gccgggaggt caattgcatc accacgcgga gtaaaaatgt acacgcgatc acggaataca
     1981 tcactgcgca tcgattcgac gaattctgtc gcatcgttta cttctccgcc ccattccatc
     2041 atcttacgca agtctttgat gcgttcttcg aattgcttat cgtgcttgcc gccttctttg
     2101 tatcgccagt gagccgcgat accgtactcg gcgttcatgt gcatttcctg agtgcggatc
     2161 tgaatctcaa gcgggcgctt atcgtcatac atcacagccg tatgcagtga ttgatagtga
     2221 ttgtcttttg gtgcggcaat gtaatcatca aactcaccgg gcacggggcg ccaggtggtg
     2281 tggataaccc ctaaagcagc ataacaagaa gggacatctg gcacgataag gcgcacagcc
     2341 cgcacatcat ataacatgcc gaaagattta tctttcttct gcatcttctt gaaaatggaa
     2401 tagatgtgct tggggcgtcc actcacttcg gctttgatgt tgttcttttc caacagcttg
     2461 attaggttgc ccttgatctc ttccaattgg cgttcgcggt ctgggcggcg ctcagcgagt
     2521 gactcgacga tctctttata tttttcaggc tgaacataac gaaaacttaa gtcttccaat
     2581 tcccacttta attgccaaat acccagtcga ttcgcgagcg gggcaaaaat gtccaaggtc
     2641 tctgcggcaa tgcggcgacg tttatgttct ggcatgtgac ccagcgtacg catgttatgc
     2701 aaacggtctg ccagtttaat cagcaccacg cgcacatctt cgcccatggc aagaaatgtc
     2761 ttgcgaaggg tttctggcac catatcttct ttgcgcccta ataaggctgg ttgaacgggt
     2821 ggttcttctt cgctctcggc gttttctgct ttttcaccac cacgcgaaac acgcggcaaa
     2881 tgagataact tggtcacgcc atcaacaagt aaacgcacca catccccaaa ctcgcggcga
     2941 atatctgcga gagttacagg tgtatcttct acggtgtcat gtaacaaacc cgccgcaata
     3001 acatcagcag gcacttttaa ttcggtcaaa atacttgcca cggcaatgca gtgattgata
     3061 tatggctccc ccgaattgcg cttctgttcg cgatgcgctt cttctgcaaa cgtataagct
     3121 cgcatcacaa gttcccggtc tgcaacagta taagactcgg ggagttgttc cattaatttt
     3181 tcgataggga tttctacgac tgccgatttc atggctgaat tttactacgc ccgcccctct
     3241 ctcggacttt gatatgcctc tgtttcctga tggctttccc tccgctgtgg cttgttataa
     3301 aaaccgctgg ctttttcaaa ccagcggttc gttttctata tggtcttcaa caatgcctga
     3361 tggctatttc gcaaacgatc tgcgatcact tcggcaatac gcttcataac tgtaaagcca
     3421 tgctcagggt tcttttccag caaggtcatg aagggttctg cagggataat aagcaggctt
     3481 gagtcttcgt ctgcttcggc gctggaggtg taatgatgcg gcgcaaccac accagaccaa
     3541 ccgaaacttc ggtacggtga agagacaaaa gacacggtga tgctttctgg gcgagaggtc
     3601 aatttgacgc gcaagacgat gctgcccttg agtaggaaat aaagcttatc ggctttttct
     3661 ccttcacgaa atacaaaatc atctttcttt actgaaactt cattgctgat gcccgcgatc
     3721 tctttgagca gagattctgg gagcccatcg aacaggctga actgggaaag aatttccgtg
     3781 ctgatcatgc aatagcctcc aatcttcagg gtgaaatagg gaaaatgggg tcgatcagtt
     3841 tcgagtttag aacgaagcgc agcaccttta tgtttataat attctacagg aaattgtcaa
     3901 tatgtcagtc acaaataatg tcacaagatt tttggattcc aggaaaatta aatatattgc
     3961 ttatgagctt ccggctgaaa aattaggcgc agtggaagcg gctcaacttt tgaatgtgcc
     4021 gcaggaacaa gttttcaagt ccatcgttac aaaacgcgag aaaggtaagc cggtattgac
     4081 ggtgatcccc ggtccgcggg tggtggattt gaaattgttg gcggggtttc tcggcgaaaa
     4141 gaaaatgcat ttgcctaccg aacgcgaagc cgagcagttg accggtctac aagcgggcgg
     4201 aatctcgccg ttggcgctta tcaacaaagg ctttcaggtg gtgatcgatg cttcggctca
     4261 aaattttgaa accatctata tttcaggcgg tcaacgtggg ctcaatattc agttgggcgc
     4321 aaaggatttg gcagggctgg tcaacgcaaa atttggcgat gtaagccgag agggataaat
     4381 aaaaatcaaa agaggaaaga agctgaagcg cttctttcct ctttttgtta cgcttgaaat
     4441 tcagctacca tttctccatt gacccataaa gaatatttcc cttcagggta actccctaat
     4501 ggaaaagcga tctcaaatgg gacgagaact tgaatgcaga tctccgccgg atttacaaca
     4561 gaatacacat ctaccaaaac tttgtggtct ttatcgggtg ggttggttgc aatgcgtaat
     4621 tgatggcagg gtgtgggtag ttcacctttt aattgcaaca tgaattgaac ggggtaactt
     4681 tccaacgagg caagctcttt tgcttcgaga gagacttctc cacgtgtcaa agagccatct
     4741 gccgggcttg ggatgtagtt gctggtttcc tgtgccgccg gtgtatggac ggtgactgta
     4801 tcgttgctaa cggggtcatc tggcgatggg gctccacatg cagatacaag aaataggaat
     4861 agaaacaatg taataaccaa acgtctcatt tttaattctc ctgcctgatt aacgaaaaaa
     4921 tgtatcgaat ggttcctaat aggaaacaaa atcacccaca ggggcatcat ttacccaaac
     4981 gtagtacctg ccgggggaat atgtgccaag cagaatccgc gcctcaaatt gttgaaacac
     5041 attgtcgcac tgcacatctg ggttgaccaa gctatatacc tggataaaga cattgaagtt
     5101 ttcatccggc gggctgatct ccactcgcag ttcatcgcaa acgctgggca tgtaaccgat
     5161 cagcccaatg gcggtccgcg gcgggctata gtcatagagc tctgcaagat ttacagaagt
     5221 aatggtgact tcatcaatct gcctgccaag gtcttctaat ttgggctcat aaggattttc
     5281 atcgggtgtg atgcgataag ggggaactgc cgttgaggtc ggctcagcag tagggacggt
     5341 gctacaagca ctgagcaaga ccgcaatcag gaagaaatta agataacgca taccgctgtg
     5401 attctccgac gcagataata tcacggtctt gcgatacaaa aaactcactc gggtagggtt
     5461 tgagtgagtt ttttgtaggg ttattttttt agcgcttcca aggcttgcaa aagaattccg
     5521 cccgtattta cgataggacc cgcattcggg tctttccaca tgaaatctcc ggtgccaagt
     5581 ccgtggtaaa ttccgctagg agtggcaata cagatcaaga catggtccgc ttgtttgggt
     5641 ggggcaggga caatatccac cacaacgagc ggaatggctt gcacagcatt ttcacctgct
     5701 ttgataaggg catcgacctt gtcatccatc gaagcgttgg gacgttccca aaccgtgccg
     5761 ccaccagaag agtgtaaggt gcgcgccttg tggtctgtat atcccacaac cgccatcacg
     5821 cctgtgggag tgccgacttc caccatgaca ccatatactt ttttggctat ttcggcaggg
     5881 ggttgcacct tcaagaagtg acgcaagaat aaccaagcct gcaaatagtg acgtggttca
     5941 aggtctgggg tttcggtaac ttggcgcaga tatttttctg cttctgcgtg attgtttgcc
     6001 gaatatgctt ttcgtgcctc aacgaacaaa gaccaaggct gtgcgtcaga gtcgcctttg
     6061 ggccattcat tgaaattaac atttcccata aactgggtac gcatatccaa aaatgggtca
     6121 gactgtttgg gttctttctt tttaaataag ttaaacataa tgtctccaaa aatgatcttg
     6181 ctggagtata tgcatttctc aatgctggta aataggacat ttggttataa aacgctttat
     6241 tctgccaacg ctttctctac tgccgcaatc gcatggatct ttgtggttgg gaaaagcggt
     6301 gctgaactat cgccttctgt aataagcaat tcgatttcgg tgcatcccaa aataatccct
     6361 tcagcgcctt ttgcca
//
LOCUS       DAOQFT010000060         6373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6035_length_6373_cov_6.962805, whole genome shotgun sequence.
ACCESSION   DAOQFT010000060 DAOQFT010000000
VERSION     DAOQFT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6035_length_6373_cov_6.962805"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..983)
                     /gene="fmt"
                     /locus_tag="PLR93_02540"
     CDS             complement(66..983)
                     /gene="fmt"
                     /locus_tag="PLR93_02540"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNH25866.1"
                     /translation="MSIRVVFMGSPDFALPSLRALVDRYNVVGVVTQPDRASGRGREL
                     KAPPVKLLAQDLNIPVMQPQKLREPEAMQQLQAWNPDLIVVAAFGQILRKDVLDLPKY
                     GCVNVHASLLPRWRGAAPINASVLAGDEETGVTIMKMDVGLDTGPMLSMEKIRIKPDD
                     TAGALFEALSTLGAKLLIETLPGYINGTVLPQPQPEEGATYAPMLKKEDGRLDFNQPA
                     VELERRIRAMNPWPGAWFEWNGNILKVSRGAVSEAKGKEVGSRLIVEGRPAVQCADGV
                     LILEEVQPPSKKVMPGKAFLSGTRNWESS"
     gene            1145..1450
                     /locus_tag="PLR93_02545"
     CDS             1145..1450
                     /locus_tag="PLR93_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25867.1"
                     /translation="MSQAPMMEVSSDDKLWAALAYAFSPLIPIILMFMEDKKNRPFIK
                     AHNMQALILGIITVVTSGFCIGILVWIYALYCAYQAYQGLEVKIPVLSDFVKNQGWA"
     gene            1565..2002
                     /locus_tag="PLR93_02550"
     CDS             1565..2002
                     /locus_tag="PLR93_02550"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNH25868.1"
                     /translation="MNEQIPQQPLAPLSPAEERQWAMFAHLGVLTNLFSGFLGPIVPL
                     AIYMIYKDRSRYVAYQSLQALIFQLIWWVGGGLLTGIVWAITGTLSAFIIGLICIPFA
                     CIISTMPLVALGYGTYAGIQCNQGQDFKYWLIGDWLRTTLTDN"
     gene            complement(2039..>3338)
                     /locus_tag="PLR93_02555"
     CDS             complement(2039..>3338)
                     /locus_tag="PLR93_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNH25869.1"
                     /translation="STGFNPETGATQEAVLPDSPAEVSAPDVALPSWDGASRINILFI
                     GLDERDLAANEGPPRTDSMIVFTIDPVSKTAGMLSIPRDMWVNIPGFGYSRINTAYPS
                     GEGAQLPGGGPELAKKTVSQFLGVPIHYYAQVDFNVFTKLVDELIAIGGCIYVEPTEK
                     MRLDPIGSGLDKIIITPGGARGLCEGWKVLAYARDRHTSGGDADRARRQQEVVLALKE
                     IIFTPENFPKFFSAAPRMYSELSYGIKTDMAFEDALKLAVLGKDISIESIKTGVIDPQ
                     QGMAIFDNTTLNGEAASILKPVMDKIRVLRDEIFTSTGPTSPLAVPNGYTDPNLFAQA
                     MQQEEARVRVMDGTFTPGLDQRAGAVFQSYGMNVTEVAPSPEAYGQTIVIVYGPKLYT
                     IKWLQATFGISDNQIRFSPDPNQTVDIEIRIGSDIAGTIP"
     assembly_gap    3339..3438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3439..3672)
                     /locus_tag="PLR93_02560"
     CDS             complement(<3439..3672)
                     /locus_tag="PLR93_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25870.1"
                     /translation="MDRFEFLKNIKRPTVSQIIFWVVTIALSITAFIVANNFTQCWTF
                     TKLPGIPPAKCGVTTDSTGFNPETGATQEAVLPD"
     gene            complement(3685..4248)
                     /locus_tag="PLR93_02565"
     CDS             complement(3685..4248)
                     /locus_tag="PLR93_02565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH25871.1"
                     /translation="MKDLLAELTSGDDERAEQAIPAIVDAGAAAIPALLELTYSENTD
                     TRWWAVRALAASPHTQTSDLISTLSDSSPEVRAAAALALSQHPGEEAVSALAQNLRDS
                     DPLAAGLAANALVKIGKAAVPSLIEAAKDGEQGIRILALRALSELRDHRAIPVMMQCI
                     QEDSAVLGYWAQMGLERLGLDMVYIKP"
     gene            complement(4251..4469)
                     /locus_tag="PLR93_02570"
     CDS             complement(4251..4469)
                     /locus_tag="PLR93_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25872.1"
                     /translation="MVSTELLEILRCPSCVREKDGLLKLHKDAWLICQDCGRKYPIVD
                     DIPVMLIEEGDKWVNTAQDALPVPPPAK"
     gene            complement(4524..>6373)
                     /locus_tag="PLR93_02575"
     CDS             complement(4524..>6373)
                     /locus_tag="PLR93_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNH25873.1"
                     /translation="LPKQLLAVLSGGFVLFIGIVLVWTVGYQLAYAGRIFPGVTVAGV
                     DLSGLSPSEAAVKLSQTLSYPNTGKVLFRDGERVWVASPAELGMVFDPTASANAAYEH
                     GRRGGLFASLSGQISARGLGMDIPPVVLFDQRVAYAYLQNIASQIDQPVAEAVLRVDG
                     TNVIAEPGRVGRALNLDATLIYLGSQLQSFRDGEVSLVIQETSPLLLDVTPQADVARA
                     MLSQSFTMTLPNVGSGDPGPWTFDVPVLANMLVFNKVDAGGVPQLQVGLDTNALRKSL
                     SELKVVVDREPQDARFIFNESKGQLEAISASTIGRAVDVEASILAINGALQRGEHSVN
                     LVVTEKQPAVTDQATGADLGIVQLVATQTTYFYGSSEARIQNIVTAAAQYDGLLVAPG
                     EVFSMGSVLGDVSLENGYAEALIIYGGRTIKGVGGGVCQVSTTLFRTVFFAGYPVVER
                     YSHAYRVPYYEMDQSGSVDGNLVGMDATVYFPLVDFKFKNDTPYWMLMETNVNVAART
                     ITWKIYSTSDGRSVTWETTGAQNTVPAPETVFEENVDLKTNELKQVDYAAEGADVTVT
                     RTVWRNGAVYFTDQIQTHYEPWAAVCQYGPGTEDPRRQARKSEVCGFSS"
BASE COUNT         1626 a         1600 c         1413 g         1634 t
ORIGIN      
        1 ttccaattga acatacatcc tccaaagatt aatttggaag ttataacccc atacctgtca
       61 atttgctacg acgattccca attccgtgtg ccggataaaa acgccttccc cggcataacc
      121 ttcttgctag gcggttggac ttcctccaaa attaagactc catccgcaca ctgtactgcg
      181 ggtcttcctt cgacgataag cctgctcccc acttctttgc cttttgcttc gctgacagca
      241 cctctactta ctttgagtat gttcccgttc cactcaaacc atgctccggg ccatgggttc
      301 atcgcacgaa ttcttcgctc caattccaca gctggttgat taaagtctaa tcgtccatct
      361 tcttttttga gcatcggggc ataggttgca ccttcttcgg gttgaggttg tggcaagaca
      421 gtgccattta tgtacccagg cagggtctca ataagaagtt tcgctcccag cgtagataaa
      481 gcctcgaaga gtgcgcccgc tgtatcgtct ggcttgatgc gaattttttc catcgaaagc
      541 atgggacctg tatccaaccc cacatccatt ttcataatgg taacgcccgt ctcttcgtct
      601 ccagccagca cagatgcatt gatcggtgcc gctccacgcc aacgtggcaa cagtgaagca
      661 tgcacattga cacagccgta tttgggcagg tctaatacat cttttcttaa gatttgacca
      721 aaagctgcaa ccacaatcaa gtcaggattc caagcttgca actgttgcat cgcttcaggc
      781 tcacgtaatt tttgaggctg catgacgggg atattgaggt cttgcgctaa aagcttaaca
      841 ggcggcgcct tcagttcacg tccgcgccca gaggcacgat ctggctgggt aaccacccca
      901 acaacattat agcgatcaac aagagcgcgg aggctcggaa gagcaaaatc gggcgaaccc
      961 atgaaaacaa cacgaataga catatgcaga ttttaccgct ggtctcaata attaaggaga
     1021 attttcagca aaacgggctc ttcgtaacgt atcagtacca ttgggtgaat tagtttggtt
     1081 gcacgttgtg tagattggtt gtaaaatact taaaattcaa acaacttatt cggaggaata
     1141 aaccatgtct caagcaccta tgatggaagt atcgagtgat gataagctgt gggcagctct
     1201 tgcttacgcc ttctcccccc ttatcccgat cattttaatg ttcatggaag ataagaagaa
     1261 tcgtccattc atcaaagctc acaatatgca ggctttgatc cttggcatta tcaccgttgt
     1321 cacttccggt ttctgcattg gtattttggt ctggatctac gctctgtatt gcgcgtacca
     1381 ggcataccaa ggcttggaag ttaagatccc tgttcttagc gatttcgtca agaatcaggg
     1441 ctgggcataa tcagtctcgc tctacaaaag acccgcgttt tgcacgcggg tcttttattt
     1501 tgctactttg cctctcctat acacgtatta attcaaagag tttgtatact attaggagaa
     1561 tatgatgaac gaacaaatac ctcaacaacc acttgccccg ctcagccctg cagaagagcg
     1621 ccaatgggcg atgttcgccc acttaggagt gttgacaaat ctattttcag gttttttagg
     1681 tcctattgtg ccattagcaa tttatatgat ctataaagat cgttcccgct atgttgcata
     1741 tcaatcgctt caagcgttga ttttccaact catttggtgg gtcggtggcg gtttattgac
     1801 aggtattgtg tgggcaatta ccggcacttt gagcgccttt atcatcgggc taatctgtat
     1861 tccgttcgcc tgtatcatca gcactatgcc attggtcgcc ctcgggtatg gaacctacgc
     1921 agggattcaa tgcaatcagg gtcaagattt caagtattgg cttatcggag attggctccg
     1981 caccacatta acggataact aataaaaaat ccccttcaaa agaaggggat tttttatatt
     2041 acgggattgt ccctgcaata tcagaaccta ttcggatctc aatatctaca gtctggttcg
     2101 ggtctgggct gaagcgaatc tgattatctg aaattccaaa ggtcgcttgc agccatttga
     2161 tcgtatatag ctttggtcca tatacaatca ctatcgtttg tccatacgct tcaggggatg
     2221 gagccacttc ggtgacgttc atgccatagg attggaatac cgctccggca cgttggtcaa
     2281 gcccgggtgt aaaggtgcca tccataacac ggacgcgggc ttcttcttgc tgcatagctt
     2341 gagcaaaaag attcgggtct gtatatccat tcggaaccgc caatggactg gtaggacctg
     2401 tagaagtaaa aatctcatca cgcaaaacgc gaatcttatc catcacaggt ttgagaatgc
     2461 tggcagcttc cccattgaga gttgtgttat caaagatcgc cataccttgt tgagggtcaa
     2521 taacacctgt ttttatgctt tcaatagaaa tatctttgcc aagcacagca agcttcaaag
     2581 catcttcaaa tgccatatct gtcttgatac cgtatgaaag ttcactatac atgcgcggag
     2641 cggcgctgaa aaacttaggg aagttttcag gagtaaaaat aatttcctta agcgccaaga
     2701 ccacttcctg ctgacggcgc gcacggtcag cgtcaccacc ggaagtgtga cggtcacgcg
     2761 catatgcaag caccttccag ccttcgcaca acccacgtgc accgcctggt gtaattatga
     2821 tcttatccaa tccagagcca atcgggtcaa gcctcatttt ttcagtaggt tcaacataaa
     2881 tacaaccacc aatagcaatc aattcatcta ctagcttagt aaaaacgttg aaatcaactt
     2941 gggcatagta atggatcggc acgcccaaga attgagagac tgttttcttg gcaagttctg
     3001 gtccgccacc cggtaattga gcgccttctc cgctggggta agccgtattg atacgactat
     3061 acccaaagcc aggaatattc acccacatat cacgcggaat ggataacatc cctgcggtct
     3121 tactcacagg gtcgatggta aagacaatca tcgagtctgt acgtggcgga ccttcatttg
     3181 ctgcaagatc gcgctcgtcc aaaccgatga acaagatatt gatgcggctt gcaccatccc
     3241 atgagggcaa tgcaacatca ggggcagaaa cttcagccgg agaatcagga agaacagctt
     3301 cttgagtagc gcccgtttca gggttgaaac cggtgctann nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnat caggaagaac agcttcttga gtagcgcccg tttcagggtt
     3481 gaaaccggtg ctatccgtgg tcacaccaca ttttgcaggt ggaatgcccg gcaacttcgt
     3541 aaacgtccaa cactgggtga agttatttgc cacaataaac gcggtgatcg aaagagcaat
     3601 agtcaccacc cagaaaataa tctgggaaac cgttgggcgt tttatatttt ttagaaattc
     3661 aaatctatcc attcactcat tacctcaagg cttaatatat accatatcca agcctagtcg
     3721 ttctaaaccc atctgtgccc aataccccaa cacagctgag tcttcttgaa tacattgcat
     3781 catgacaggg atagcacgat gatcgcgcag ttctgagagt gctcgcaatg ccaaaatacg
     3841 gatgccctgc tccccatctt ttgccgcctc aataagactt ggtacagcag cttttccgat
     3901 cttgactaaa gcattcgccg caagccccgc tgcaagtggg tcactatctc tcaggttttg
     3961 agccaacgct gaaacagctt cttcgcccgg gtgctgagac aatgccaacg cggcagcggc
     4021 acgtacctca gggctggaat cacttagggt ggaaatcagg tctgaggtct gggtgtgagg
     4081 cgaagcggcg agggcacgca ctgcccacca acgtgtgtct gtgttttcag agtaggtcaa
     4141 ttccaataag gctggaattg ccgccgctcc tgcatctaca atagcgggaa tggcttgttc
     4201 tgcgcgttca tcgtctccgc ttgtaagctc agcgagcagg tctttcattt ttattttgca
     4261 ggcggcggga cgggcaacgc atcttgtgcg gtgttcaccc atttatcgcc ttcttcaatc
     4321 aacatcacgg ggatgtcatc cacaatggga tattttcgtc cgcaatcctg gcagatgagc
     4381 caggcatctt tgtggagttt cagcaaaccg tctttttcgc gcacgcagct ggggcagcgc
     4441 aagatttcga gtaattcagt actgaccatt gtttctcctt gttgggaaaa gattgtacca
     4501 tgtaggtttt gacaagattt tatttatgaa ctaaagccac aaacttcgct cttccttgct
     4561 tgtcttcgcg gatcttctgt gccgggacca tattgacaaa ccgcagccca cggctcatag
     4621 tgagtttgaa tttggtcggt gaaatatact gccccattac gccataccgt gcgtgtgaca
     4681 gttacatcag caccttcagc ggcgtaatct acttgtttaa gttcgtttgt ctttaaatct
     4741 acattttctt caaagaccgt ttctggggca gggaccgtat tttgtgctcc cgttgtttcc
     4801 caagtgaccg aacgtccatc tgatgtggaa tagattttcc acgtaatggt gcgcgcagct
     4861 acattaacat tggtttccat caacatccag taaggcgtat cgtttttgaa cttaaagtcg
     4921 accaagggga aatagactgt ggcgtccatg ccaaccaagt ttccgtccac tgagccgctt
     4981 tgatccattt cataataagg cacacgatag gcgtgagaat aacgttccac cactgggtac
     5041 cctgcaaaga acacggtgcg gaacaaggtg gtgctcacct gacacacacc gcctcccaca
     5101 cctttgatgg tacgaccgcc ataaataatg agtgcttcgg cataaccatt ttccaagctg
     5161 acatcgccaa gcacactgcc catggaaaat acttcaccag gagcaacgag cagaccatca
     5221 tactgagcag ctgcggtgac aatattttga atgcgcgctt cgctggaccc gtaaaaataa
     5281 gtggtttggg tggcaacgag ctgaacaatg ccaaggtctg cgccagtggc ttgatctgtc
     5341 actgcaggct gtttttctgt aacaaccaaa ttaacgctgt gttctccgcg ttgtaacgca
     5401 ccgttaattg ccagaatact agcctccacg tccacagcac gtccaatcgt ggaagcagag
     5461 atcgcttcaa gttgtccctt tgactcgttg aagataaagc gtgcatcttg aggctcacgg
     5521 tctacaacga ccttcagttc actcaacgat ttgcgtaaag cgttcgtatc caaaccaact
     5581 tgtaattgcg gcacaccgcc tgcatccact ttgttgaaga ccaacatatt tgccaaaaca
     5641 ggcacatcaa aagtccatgg accggggtca ccagaaccga catttggtaa tgtcatcgta
     5701 aatgactggc taagcatggc acgcgccaca tctgcttgag gcgtaacatc caacaaaagt
     5761 ggggatgttt cttgaatcac gagagaaact tctccatctc gaaaactttg caactgcgaa
     5821 ccaagataga tcaaggttgc atcgaggttg agtgcgcgtc ccacacgccc cggctctgcg
     5881 atcacattag ttccatctac tcgcagaaca gcttcagcta caggctggtc gatttgagag
     5941 gcgatgtttt gcaagtatgc atatgcaacc cgttggtcaa agagcacaac cggtggaata
     6001 tccatgccaa gtccgcgcgc gctgatttgt cctgatagtg aagcaaacaa cccgccccga
     6061 cgtccatgtt catatgcagc attggcactg gcagttgggt cgaacaccat gcctaactct
     6121 gcaggcgatg ccacccacac gcgctcacca tctctgaaaa gaacttttcc cgtattcggg
     6181 taagaaagtg tctggctcaa ctttacagca gcctcactgg gggaaagacc cgaaaggtcc
     6241 acaccagcaa ccgtcactcc agggaaaatg cgtccggcgt aggcaagttg atatccaact
     6301 gtccacacaa gaacgatgcc aataaaaagc acgaacccac cgctcaacac ggcaagcaac
     6361 tgcttgggca aat
//
LOCUS       DAOQFT010000061         6334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6088_length_6334_cov_8.279503, whole genome shotgun sequence.
ACCESSION   DAOQFT010000061 DAOQFT010000000
VERSION     DAOQFT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6088_length_6334_cov_8.279503"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..423
                     /locus_tag="PLR93_02580"
     CDS             49..423
                     /locus_tag="PLR93_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25874.1"
                     /translation="MSTCALSVSDDKKFIIMKITGQVNSQDMYQHTLEAHQLGRSLHL
                     RAFLVDLTDAINTQSVVEKYDFVYKKVQEDDELLHFVRMAMWVKKGDHSHDFIETLAN
                     NTGEQAKIFHDQQAAIRFLLEL"
     gene            complement(503..1087)
                     /locus_tag="PLR93_02585"
     CDS             complement(503..1087)
                     /locus_tag="PLR93_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25875.1"
                     /translation="MNKNLRILSVLGVQVLIVAALLFGAQKLTEYQNANVPPKTYVYK
                     NIYFDYPGNWELKLEESDQGGELTVTPAKGSPEEKMFGQFYIMFLPDIAFDENWKQTL
                     ADEASCSGKENTQWHQMIEGDDFSGFECVWKDPERAFPSWEFFLYNEGEQTAISILAL
                     PVNDTATEALQSPETVQQVFPDLLALAKSLRISK"
     gene            complement(1084..2298)
                     /locus_tag="PLR93_02590"
     CDS             complement(1084..2298)
                     /locus_tag="PLR93_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNH25876.1"
                     /translation="MTKTVAIIGGGPAGLMAAEVLSQNADLQIDVYDAMPSLGRKFLM
                     AGKSGLNLTHSEPFEKFVSRYSRKEVEAWLHAFTPDNLRTWAKELGVDTFVGTSGRVF
                     PVGMKASPLLRAWIQRLSASGVTFHLRHKLSGLPADTSLHFETPNGNVTVKADAVILA
                     LGGGSWRKLGSDGAWVEWLSQAGVKVEPLRPANCGFDVAWSSVFKEKFDGQPVKSVIV
                     SFGAFHQQGEFIITQEGVEGSLVYAASALLRDAIEASGTATMTLDLAPDRTEAQLVER
                     LSKPRGSRSMASHLEKAVGIKGVKAGLLREFVPKEEFTNEVRLAFYIKNLPVPLVATR
                     PLDEAISSAGGVRFEMLDQGLMLKHHPGIFCAGEMIDWEAPTGGYLLTACFASGRWAG
                     AGVKKWLSGDME"
     gene            complement(2354..2521)
                     /locus_tag="PLR93_02595"
     CDS             complement(2354..2521)
                     /locus_tag="PLR93_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25877.1"
                     /translation="MILNRTTRNILIAISAGVATGIFLNMLSMSLPSLIVGVVVATGV
                     TFALVHSRKDS"
     gene            complement(2527..3588)
                     /locus_tag="PLR93_02600"
     CDS             complement(2527..3588)
                     /locus_tag="PLR93_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH25878.1"
                     /translation="MQRRSKGVVQLEWVCPNCDTRNPGPVKNCQNCGAPQPDNVQFQR
                     AADEKLITDEKVVQAAKAGADIHCGFCGTRNPATAETCSQCGADLKEGKARQAGQVMQ
                     AAPSTPKVVTCSNCGSENSGSAKTCTNCGAPIKLPQAKAPAPIAASAQAGKVAAQANK
                     KPVNWLVAGGIGAVLVMCCIAVLMLFVFPSRSVTGTVSGVSWQTSVPVQEVQPVNYSN
                     ERGNPPSDAYNVSCRTDSQEVCEDKTIDKGNGYAEVVTECHTVKDQYCSYTVDEWTTI
                     QTYDLNGTDLNPEYSSPNISSDQRLGNSSTQLQVYFSTSDGELTYSPNSVTEFQQYQP
                     GSQWTLKLNAVGSVLSVER"
     gene            complement(3712..5727)
                     /locus_tag="PLR93_02605"
     CDS             complement(3712..5727)
                     /locus_tag="PLR93_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH25879.1"
                     /translation="MKKVFLFVSLLVITSLSCKALSPKPTEQTTPPTLSPASGDTQSD
                     TAAKLQELGGVTCEDAPDLTCVTIQVPLNHYDSANTETIDVVFGVAPATGERYGMFVQ
                     AFPGGPGGEGISSSYMGYFDPTVLEHYDMVYYDQRGLGLSSPLSCPKAYAADFVEYLT
                     TPDTAGLEGLDTPEEQKTAIDDSRAYAQNCIAEMGIDPAKLAFYGTDQVAEDIDSFRA
                     LLGDEKLWIYGVSYGTAVAQTYATEHPDRVAGMILDGTINLTLDGVQGSLAQEKAFDK
                     VLVAVLNACDEDEVCHADMGGENALAVYDKFAAQVSKDPIEYEYPLPNGEKVKGVFTF
                     NALEFTTAYQMYSLTTRMIFLRALAAANQGDIIPMLRLMYENATIDPATFEYIGDPTF
                     SDTMFLSVLCTDDTFFTGTQEERIQQSIEAGQASNGTVPRLDGSVYTGIDCAFWPSSP
                     KEEVTREPLKLDGVPVLVLNATLDPATPFEEGKFVAENLADGYHIYVEGGVHSIFGYG
                     YACPDQYVTDLLVDGKVPDQREIQCTDWENPVYANYSPNLPASANDFADALDMMIYLD
                     ENFYYLPEVYLNDWSEEKSIGCTYGGTYTFAPSEKGEDHTYENCQMMPGVTVNGTGVF
                     DYNAGVFSMAVNISGDKTGELNYSYDFNTGTTSVNGTFEGEPVDLSQ"
     gene            complement(5747..>6334)
                     /locus_tag="PLR93_02610"
     CDS             complement(5747..>6334)
                     /locus_tag="PLR93_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNH25880.1"
                     /translation="DLLRKGLKRVMVIDYDAHHGNGTQAAFLHEERLGFVSTHQWGIY
                     PGTGWIDDAPHANQRIVNVPLPAYAGDKTFVRIADEVFAPMVRAFKPEMLLISAGFDS
                     HWNDPITSLGLSTRGFYDVSKKLVEMAEEHCGGKIVFVLEGGYDPRNVANGVGAVFDA
                     LTSRPLGNEAGDASPYKEPDFESRLKEIRQKHDLI"
BASE COUNT         1553 a         1618 c         1510 g         1653 t
ORIGIN      
        1 aatataaaat acaatggaag taggtcttaa tagggatagg agaccactat gagcacttgt
       61 gcgctttctg tttcagatga taaaaaattc atcatcatga aaattaccgg gcaggtcaat
      121 agtcaggaca tgtaccagca tacattggag gcgcatcaat tggggcgttc ccttcatctg
      181 cgcgcctttc tcgtcgacct gaccgatgcc atcaataccc aatccgtagt cgaaaaatat
      241 gattttgtct ataaaaaagt tcaggaggat gacgagttgc ttcatttcgt tcgtatggcg
      301 atgtgggtga aaaagggcga ccattcccat gattttatcg aaacccttgc caacaacaca
      361 ggggagcaag ccaaaatatt tcacgaccaa caagcggcta tccgcttttt acttgaactg
      421 tagaacgtgc tccatccatc aaaaaggaca tccctcgagc catatcgagg gatgtccttt
      481 tgatagacaa gacaaaaatt gactacttag aaatgcgtaa actttttgca agcgccagca
      541 ggtctgggaa tacttgctgg acagtttcag ggctttgtag agcttcggtt gccgtatcgt
      601 tgaccggcaa ggctagaata ctgatggcgg tttgttcgcc ttcgttgtag aggaaaaact
      661 cccaactggg gaaggctctt tcggggtctt tccacacaca ctcaaagccc gagaaatcat
      721 ccccttcgat catctgatgc cactgagtgt tttctttccc agaacaactg gcttcgtctg
      781 ccagagtttg cttccagttt tcatcaaagg caatatcggg cagaaacata atatagaact
      841 gcccaaacat tttttcttcc ggcgagccct tcgcgggtgt gacggtcagt tcgccgcctt
      901 ggtcgctttc ttccaacttg agttcccaat tgccggggta atcaaaatag atattcttat
      961 acacataggt cttgggtggg acgtttgcat tctgatactc agtcaacttt tgtgcgccga
     1021 acaataatgc cgcaacgatc aacacttgca ccccaagcac gctcaaaatt ctaagattct
     1081 tattcattcc atgtctccag ataaccattt ttttacgcca gcacctgccc aacgtccgct
     1141 tgcaaaacaa gcagtcagca aataaccgcc cgtgggagct tcccaatcta tcatttcacc
     1201 ggcacagaaa atgccggggt gatgttttag catcaagcct tgatccaaca tctcaaatct
     1261 cacgccgcca gcagaactaa tcgcctcatc caacggacga gtcgcaacca gtggcacagg
     1321 caggttcttg atatagaaag ccagccgtac ttcatttgta aattcttctt tgggtacgaa
     1381 ctcacgcaac aacccggctt tgaccccttt tatgcctacg gctttttcca aatggcttgc
     1441 catcgagcgc gacccacgtg gttttgataa ccgctctacc aactgagctt ctgttcggtc
     1501 tggtgcaagg tctagagtca ttgtggctgt accgcttgct tcaatcgcat cacgtaacaa
     1561 tgctgaagcg gcgtatacca aacttccctc cacgccttct tgtgtgatga taaattcgcc
     1621 ctgctgatga aacgccccaa acgatacgat cacagacttg actggctgcc cgtcaaactt
     1681 ttctttgaat accgaactcc atgccacatc aaacccgcaa ttggctggac gtaacggctc
     1741 gaccttaacc ccggcttgac tcaaccattc gacccatgcc ccgtccgagc cgagtttgcg
     1801 ccaactgccg ccgcccaaag caaggatgac cgcatcagct ttcacagtga cattgccatt
     1861 gggtgtttca aaatgtagag acgtatcggc tggcaacccg ctcaatttgt ggcgcaggtg
     1921 aaaggtcacg ccagaggcgc tgagtctttg aatccacgcc cgcagtagtg ggctggcttt
     1981 catcccaacc ggaaagactc gccctgaagt tcccacaaac gtatctacac cgagttcttt
     2041 tgcccaggta cgcaaattat cgggcgtgaa ggcatgcagc caagcttcaa cttctttgcg
     2101 gctgtagcgc gaaacgaatt tttcaaaagg ttcagagtgg gtaaggttta gcccgctttt
     2161 gcctgccatt aagaatttgc gccccagcga aggcatggca tcatatacat ctatttgaag
     2221 gtcagcgttt tgtgataaga cctctgctgc cattaagcca gccggtccgc cgccgatgat
     2281 tgctacggtt ttcgtcatgg ggttcattat aaagagaaac tcccgaaagg gagtttctct
     2341 ttatatataa cgattaacta tctttgcgag aatgcacgag cgcaaatgtc acacccgtgg
     2401 caacgacaac gcccacgata agcgaaggca gcgacatgga tagcatgtta aggaagatgc
     2461 cggttgcaac cccggctgaa attgcgatca agatatttcg ggtcgttcta ttgagaatca
     2521 tagggcttat cgttctacgc tgagcacact gcccacggca tttaacttca atgtccactg
     2581 actgccgggt tgatattgtt gaaactcagt gaccgagttg ggcgaatagg tcaactcgcc
     2641 atcgctggtg ctgaaatata cctgtaactg ggtagagcta ttcccgagac gttgatcgct
     2701 ggaaatgttg gggctggaat attcagggtt caggtctgtt ccgttcaggt cataggtctg
     2761 aatggttgtc cactcgtcga cggtgtaact gcaatattgg tcttttacgg tgtgacattc
     2821 tgtcactact tcagcgtagc cattgccttt gtcgatggtc ttgtcttcgc agacttcttg
     2881 actatcggtt cggcaggaaa cgttatacgc atcagagggg ggatttccgc gttcgtttga
     2941 ataattgacc ggttgaactt cttgcactgg aaccgatgtt tgccaagaga caccagagac
     3001 tgtgcccgtg acagaacgag aggggaacac gaacaacatc aaaacagcga tacagcacat
     3061 caccaaaacg gcgccaatgc cgcctgccac caaccagttg actggttttt tatttgcctg
     3121 cgcagcaacc ttgcccgctt gtgcagatgc cgcgatgggg gcgggcgctt tggcttgtgg
     3181 caatttgatg ggcgcaccac agttcgtaca ggttttggca ctgcctgagt tttcgcttcc
     3241 gcaattcgaa caggtgacaa ctttcggcgt ggaaggagcc gcttgcatca cttgcccagc
     3301 ctggcgggct ttgccttcct tcaaatccgc gccgcattga gaacaagtct ctgctgtggc
     3361 ggggttgcgc gtcccacaga atccgcagtg gatgtctgcg cccgctttgg cagcttgcac
     3421 caccttttca tcggtgatca atttttcatc agcggctcgc tggaattgga cattatcagg
     3481 ttggggcgcg ccgcagttct ggcagttctt gacgggtccc gggttgcgcg tgtcgcagtt
     3541 tgggcatacc cattccaact gcacaacgcc tttacttctt ctttgcatgg tctactcctt
     3601 atcaatcatt gcgtatgtgc caagtgtaca acaatctgtg catggattga atggcatcat
     3661 taaaaacacc ttcgagggta tcgaaggtgt ttaggcatcg caatcaacgt tctattgcga
     3721 taagtctacc ggttctcctt cgaacgtgcc gttcacagat gtggttcctg tattgaaatc
     3781 atacgagtaa ttgagctctc cggttttatc tccgctgatg ttgactgcca tgctgaaaac
     3841 gccggcgttg taatcaaaaa cgcctgtgcc gttgacggta acgcccggca tcatctggca
     3901 attctcatag gtatggtctt cgcctttttc actgggtgca aaggtgtacg tgccgccata
     3961 ggtgcagccg atggattttt cttcagacca atcgttgaga tacacttcgg gtaaataata
     4021 gaagttctca tcgagataga tcatcatatc caatgcgtct gcaaagtcat ttgcacttgc
     4081 gggtaagttg ggagagtagt ttgcgtacac ggggttttcc cagtctgtac attggatctc
     4141 gcgttggtct ggaactttcc cgtctactaa aaggtcggtc acatattggt cggggcaggc
     4201 ataaccatac ccgaagatgg agtgaacgcc gccttccaca taaatgtgat agccatctgc
     4261 taagttctca gccacaaact taccttcttc gaacggagta gctgggtcga gagttgcatt
     4321 caaaaccaaa acaggcacgc catctaactt caacggctca cgtgtaactt cttccttggg
     4381 cgaactaggc cagaaggcac agtcgatgcc ggtgtagacc gagccgtcca aacgtggaac
     4441 ggttccgttc gacgcctgac ccgcttcaat cgattgttga atgcgttcct cttgggtgcc
     4501 ggtgaagaag gtatcgtcag tacatagcac gctgaggaac atcgtatcgg agaaagttgg
     4561 gtcgccgatg tattcaaagg ttgccgggtc tatggtcgca ttttcgtaca tcaagcgcaa
     4621 catggggatg atgtcgcctt ggtttgcggc agccaatgct cttaagaaaa tcatacgggt
     4681 tgtaagagag tacatctgat acgccgtggt gaattcaaga gcattgaagg tgaacacacc
     4741 ttttactttt tcaccgtttg gtaacggata ttcatattca atcgggtctt ttgagacttg
     4801 ggctgcaaac ttatcgtaca cagccaacgc attctcgccg cccatatctg catgacaaac
     4861 ttcgtcttca tcacaggcat tcaagactgc taccaatact ttgtcgaagg ctttctcttg
     4921 tgcaagtgaa ccctgcactc catccaatgt caagttaatg gtgccatcca gaatcatgcc
     4981 tgccacgcgg tctgggtgtt cggtggcata ggtttgagcg actgccgtgc catagcttac
     5041 gccatagatc caaagctttt catcacccaa caaagcacgg aatgaatcaa tatcttccgc
     5101 tacctggtcg gtgccataaa aagctaactt ggcagggtca atgcccattt ctgcgataca
     5161 gttttgagca taggcacgcg aatcatcaat ggcggttttc tgttcttcgg gtgtatccaa
     5221 accttccaac ccggcagtat cgggcgtggt gaggtactca acaaaatctg cggcatatgc
     5281 cttcgggcat gagagcgggc tagaaagccc aagcccacgc tggtcataat agaccatatc
     5341 gtaatgttca agaaccgttg ggtcgaaata acccatatac gatgaactaa tgccttcacc
     5401 gccaggtccg cctgggaagg cttgtacgaa catcccatag cgttcacctg tggcaggtgc
     5461 cacaccaaag accacgtcaa tggtttctgt gttggcagaa tcataatgat tgagtggaac
     5521 ctgaatggtc acacacgtga ggtcaggtgc atcttcacaa gtcacaccgc ctaattcctg
     5581 aagcttggcg gcagtatcac tttgagtatc tccacttgca gggctgagtg tgggaggagt
     5641 ggtctgttct gtgggcttcg gtgataaagc tttacaactt aaagacgtga ttacgagtaa
     5701 cgatacgaac aaaaaaactt tcttcatata aatttccttt tgatgtttag ataaggtcgt
     5761 gcttttggcg gatctctttc aagcgtgact cgaaatccgg ttctttatag ggagatgcat
     5821 cgcccgcctc attccctaag ggacggctgg tcagcgcatc gaaaactgcg cctacgccgt
     5881 tcgcgacgtt tcgcggatca tacccacctt ccaatacaaa aacgattttt cccccacaat
     5941 gttcttctgc catttccacc aattttttcg acacgtcata aaagccacgt gtcgacaatc
     6001 ccaatgaagt gatggggtca ttccaatgcg agtcaaaccc tgctgagatc aatagcatct
     6061 cgggcttgaa ggcacgcacc atcggcgcaa agacttcgtc tgcgatgcgc acaaaggtct
     6121 tgtcacctgc ataagccggc aacggcacat tgacaatgcg ttggttggcg tgaggcgcat
     6181 cgtctatcca gccggtgccg gggtaaattc cccattgatg cgtggagaca aatcccagcc
     6241 gttcttcgtg caaaaatgct gcttgtgtcc cattgccatg atgtgcgtca taatcgatca
     6301 ccatcacacg cttcaagcct tttctcagca aatc
//
LOCUS       DAOQFT010000062         6289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6159_length_6289_cov_8.090472, whole genome shotgun sequence.
ACCESSION   DAOQFT010000062 DAOQFT010000000
VERSION     DAOQFT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6159_length_6289_cov_8.090472"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLR93_02615"
     CDS             complement(<1..364)
                     /locus_tag="PLR93_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucC family protein"
                     /protein_id="HNH25881.1"
                     /translation="MFIKRLQLALIHTAVAMTLVPINSTLNRVMIFDLGVSKTLFTLL
                     AIFPYLLSPIQVAIGSFSDRHPILGYRRAPYIFAGLLLCVIGVAVSPQIAILISNNFT
                     VGVAAGILAFGLWGMGYNL"
     gene            complement(504..1043)
                     /locus_tag="PLR93_02620"
     CDS             complement(504..1043)
                     /locus_tag="PLR93_02620"
                     /inference="COORDINATES: protein motif:HMM:NF017987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HNH25882.1"
                     /translation="MFQTNISLSPEMTANERNQAIREAYNQDRSQMTLMLAHQLRIPE
                     VDILRALEGDTARELDFARWEEIIRAFEALGEVTVIVSNGSTTIESFGQFGGFSNAQG
                     FLNVRSKSLDMHIRGWELASVFAFRKPSHLDKHESLSFQFFDKRGNSAFKVFLNFGGK
                     DPAPELVEAYNELIGRFSK"
     gene            1207..1908
                     /locus_tag="PLR93_02625"
     CDS             1207..1908
                     /locus_tag="PLR93_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNH25883.1"
                     /translation="MSVSLAMQRYAAEIYRLQQDHEQVPLSLLSSHVDASAQAISNMV
                     KRLQKLGYLVHEPYRGVRLTETGEKIAMPSLRRHRLTEVFLVKVMKYDWATAHELSDT
                     FEKGVNDEIENRMDELAGRPTRCPHGEPIPSKDGFMPVVKDMPLIDVPSGSDCVISRV
                     RTHDLEKLNYIGELGLTPGKQFHLFSCAPFKGPLRLQMKPHDHLIGYELAASLWVEVT
                     LKGEGNKVPTSLASK"
     gene            complement(1957..>2556)
                     /locus_tag="PLR93_02630"
     CDS             complement(1957..>2556)
                     /locus_tag="PLR93_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNH25884.1"
                     /translation="VHLATPHGVVLQQPAFSKYYLHLGEISSYPPGYKENAGIFCHTN
                     PWIMIAEAKVGRGDKALDYYLRINPSAREGIGDLHRCEPYVYAQMIAGKDAATHGEAK
                     NSWLTGTAAWNYVAITQWILGIRPTYEGLQVSPVVPVGWKSFEAVREFRSVRYNIKVE
                     RSGAGNQVKLEVDGKAIAGNIIPLAANGTKEVRVRVILS"
     assembly_gap    2557..2656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2657..4225)
                     /locus_tag="PLR93_02635"
     CDS             complement(<2657..4225)
                     /locus_tag="PLR93_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNH25885.1"
                     /translation="MRYGHFDDTNREYVIDTPETPLPWINYLGSESYFGLISNTAGGY
                     SFYKDARLRRLTRYRYNNSPLDMGGRYIYLRDNNAADSARVWSPSWMPARTPLDFYEC
                     RHGMGYTIIRSKFNGIESQTRYFVPMGENLEIWELTLTNHRDSAADISIFSAIEFNLW
                     DAMDDSSNFQRNYSIGQVEVVDDIIYHKTEYRERRDHFAYFACSEKLSGFDTQRESFL
                     GSYRGWDNPKAVEAGQATNSIAHGWQPIGSHHVKVQLKAGEQKKIIFVLGYHENPKDA
                     KFDPPESQIINKRTVLPIISKYLKTAEVDKAFEGLRSHWTNLLNKFQVNTPDEHTDRM
                     VNIWNAYQTMITFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHLIPERARERILDLA
                     ATQLETGGAYHQYQPLTKRGNNDVGSGFNDDPLWLVLAVAAYVKETGDFSILNEPVQY
                     DNKPGSEEPLYGHLVRSIQYTLDRLGPNQLPLIGRADWNDCLNLNCFSDSPGQSFQTT
                     TNKDGKVAESVFIAG"
     assembly_gap    4309..4408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4409..5571)
                     /gene="xylB"
                     /locus_tag="PLR93_02640"
     CDS             complement(<4409..5571)
                     /gene="xylB"
                     /locus_tag="PLR93_02640"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNH25886.1"
                     /translation="MNYFLGIDTSTTSSKALLIDSNGVVIAVAAASHTLQTPKPLWSE
                     QNPREWWDAVAASIKSVLEKAGVSGERVVAVGLTGQMHGLVLLDENGSVLRPAILWND
                     QRTQSQCDEIHQVIGKEKFIQITGNVALTGFTAPKILWVKENEPSVYARAKHVLLPKD
                     YIRFCLTGEYAMDKADGAGTVLFDLKVRDWSDEVLAALGIPREWMPRTFEGTAFTGKV
                     TEEAARLTGLKAGTPVAAGGGDQAAGAVGVGAVEPGIVGLTVGTSGVVFATTPSALIE
                     PEGRLHAFCHAVPGMWHFMGVMLSAAGSLQWYRDTLAPNVSFDELVKEAESAPAGSEG
                     LLFLPYLSGERTPYPDPLARGSFVGLTLRHTRAHMTRAVLEGVAFGLKDSFTL"
     gene            complement(5577..>6289)
                     /locus_tag="PLR93_02645"
     CDS             complement(5577..>6289)
                     /locus_tag="PLR93_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25887.1"
                     /translation="YHFYGGHRLWHAPEVPSRTYLPDDSPVDITQIENTLHIVQEVEA
                     KTGLQKSFDIQMQGDCASLSITHRLTNKGLWSATTALWAITQFKPGGLGILPQTVENT
                     GVLPNRRLTFWPYTDPSEPAVHLGKNYILINANMQGAFKVGFSNPRGWQAYWWNGVLF
                     VKRAKYEADAEYFDYGSSSESYCNHEILELETLSPITTIEPGAEATHVETWEMYQGIE
                     RPQNEQDVQALVERLELE"
BASE COUNT         1495 a         1500 c         1546 g         1548 t
ORIGIN      
        1 agaggttata gcccatgccc cataaaccaa aggcgagaat gccggcagcc accccaactg
       61 taaagttgtt gctaattagg atggcgatct gcggtgagac ggcaacccca atcacacaca
      121 aaagcagacc agcgaaaatg tatggcgcgc ggcgataccc tagaatgggg tgacggtctg
      181 agaaggagcc aatggcaact tgaatggggg aaagcaaata gggaaagatg gcaagcagtg
      241 taaaaagggt ttttgagacg cctagatcga agatcatgac ccggttgagg gtgctgttga
      301 tgggcacaag cgtcatggca acagcggtat ggatgagggc aagttgaagt cttttgatga
      361 acatagcatt tcctgaggtt gaggattaaa caaaaggaag ggcgatttgg gcgccctcac
      421 ctcttggttg atattgtagg ctcaagtttc cttaggagta tatagattta tatagaattt
      481 cactagagtt ttctgaggaa gatttacttt gaaaaacgcc cgatcaattc gttataagct
      541 tcgaccaact caggagcagg gtctttgccg ccaaagttga gaaagacttt gaaggcagag
      601 ttgccgcgtt tatcgaagaa ttggaaggaa agcgattcgt gcttgtcgag gtggctcggt
      661 ttgcggaaag cgaagaccga tgccagctcc caaccgcgaa tgtgcatatc cagtgatttg
      721 ctgcggacgt tgagaaagcc ttgagcattg gaaaaaccgc cgaactgacc gaacgattcg
      781 atggtggtcg agccgttcga gacgatgacg gtcacctcgc ccaacgcctc aaatgcgcgg
      841 atgatctcct cccagcgcgc aaaatcaagc tcacgggcgg tgtcgccttc gagcgctcgc
      901 agaatgtcca cttcggggat gcgtaactgg tgggcaagca tgagtgtcat ttgagagcgg
      961 tcttggttat acgcttcacg aatcgcttgg tttcgctcgt tggcggtcat ttcggggctg
     1021 aggctgatat tggtttggaa catgaaatat tctcctaaaa tgtagtgttg accatacttt
     1081 gtttgataag tggcgcgtag tataatcacg gcggtacgaa taatctagat aattgttata
     1141 aaatttatct atatccagtt gaagccagtt ttcaacccaa ttgaagattt tggagtgttt
     1201 taaagaatgt ctgtaagttt ggctatgcag cgttatgccg cggagatcta ccgcctgcaa
     1261 caagaccatg agcaggtgcc gctttcgctt ttaagttcgc atgtggatgc ttctgcacaa
     1321 gccatttcca acatggtcaa gcgtttgcaa aagctgggtt atcttgtaca tgaaccctat
     1381 cgcggagtcc gccttactga aactggcgaa aagatcgcca tgccttccct gcgtaggcat
     1441 cgcctcactg aggtctttct cgtcaaagtg atgaagtacg actgggcaac tgcccacgaa
     1501 ctttctgaca ccttcgaaaa aggcgtcaac gacgagatcg aaaaccgcat ggatgaactt
     1561 gccgggcgcc ccacacgttg cccgcatggc gagccgattc cttccaaaga tggcttcatg
     1621 cctgttgtca aagatatgcc attgatcgac gtcccctctg gttctgattg cgtcattagc
     1681 cgcgtccgca cccatgacct tgaaaaatta aattacattg gcgaattagg gcttaccccc
     1741 ggcaagcaat ttcacttgtt tagttgcgcc ccgttcaaag gtccgttacg attgcaaatg
     1801 aaaccgcatg accatctcat cggttacgaa ctcgccgctt ccctctgggt ggaagtgacc
     1861 ttgaagggcg aaggaaataa agtgccaaca agcctggcgt ctaaataaaa aaatacatcc
     1921 cgatttcggg atgtattttt tttatcaaga aaatcactag ctcaatatca cacgcacacg
     1981 aacttctttg gttccattcg cagccaaggg gatgatattt cctgcgatgg cttttccatc
     2041 cacttccagc tttacctgat tcccagcacc gcttctttcg accttgatgt tgtagcgtac
     2101 gcttctaaat tctctcaccg cttcgaacga tttccaccca accggaacaa ccggcgagac
     2161 ttgcaaccct tcatacgtcg ggcggattcc aagaatccat tgcgtgatgg cgacataatt
     2221 ccaagcggca gtgccagtga gccaggaatt ctttgcctcg ccgtgagtcg cagcatcttt
     2281 gcccgcgatc atttgagcat agacatacgg ctcacagcga tgcaaatccc ctatgccttc
     2341 ccgcgccgat gggttgatgc gtaggtaata gtcaagcgct ttatctccgc gcccaacttt
     2401 tgcctctgcg atcatgatcc atggattggt atggcagaaa atacccgcgt tctccttgta
     2461 gcccggcggg taggaagaaa tctcacccaa gtgcaaataa tatttgctga aggctggctg
     2521 ttgtaatacc actccgtgcg gtgtcgccaa atggacnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnaccg gcaataaaaa cactctctgc cactttgccg tctttgttgg
     2701 ttgtggtttg gaaggattga ccaggtgaat cagagaagca gttgaggttc aggcagtcgt
     2761 tccaatctgc acgcccgatc aacggcagtt gattgggtcc gagcctgtcg agtgtgtatt
     2821 gaatcgagcg gactaaatgt ccgtaaagcg gctcttcact gccgggcttg ttatcatact
     2881 gaacgggttc gttgagaatg ctaaagtcgc cggtttcttt gacatatgcc gcaaccgcca
     2941 acaccaacca caatgggtcg tcgttgaagc ccgagccgac atcattgttg ccgcgtttgg
     3001 tcagtggttg atattgatga taggcgccgc ccgtttcgag ttgggtggct gccagatcca
     3061 aaatacgttc acgggcgcgt tcagggatga ggtgaacgaa accgagcaaa tcttgatttg
     3121 agtcacggaa gcccatgcca cgcccaatgc cgctttcaaa gtatgaagcc gagcggctca
     3181 tgttgaaggt gatcatggtt tggtaggcat tccaaatatt gaccatgcgg tctgtatgtt
     3241 catcgggagt gttcacttga aacttattga gcaaattcgt ccaatgagag cgtaaccctt
     3301 caaaggcttt atcgacttca gcggttttta gatacttgga aatgatcggc aagacggtgc
     3361 gtttgttgat gatctgtgat tcaggcgggt cgaacttggc gtctttgggg ttttcgtgat
     3421 acccaagcac aaagatgatc ttcttctgtt cgccagcctt taattgcact ttgacatggt
     3481 gtgacccaat gggctgccag ccatgcgcaa ttgaattcgt agcttgacct gcttccacgg
     3541 ctttggggtt atcccatccg cgataggagc cgaggaaaga ttcacgttga gtatcaaagc
     3601 cgctcagttt ttcggagcag gcaaaatagg caaagtggtc gcggcgttca cgatattcgg
     3661 tcttgtgata aatgatatcg tccaccactt ctacttgacc gatggagtaa ttgcgctgga
     3721 agttgctcga gtcatccatg gcgtcccata gattgaattc gatggcagag aagatggaga
     3781 tatcagcagc ggaatcgcga tggttggtga gagtcagctc ccaaatttcg aggttctcgc
     3841 ccatgggcac gaagtatctt gtttgagatt cgatgccgtt gaacttggaa cggatgatgg
     3901 tatagcccat gccatgacgg cattcgtaga aatctaacgg cgttcgcgcg ggcatccagc
     3961 ttggcgacca gacccgagcg gagtctgcgg cgttgttatc cctcaaatag atgtaacgcc
     4021 cgcccatatc caatggggaa ttgttgtagc gatagcgagt caagcggcgc aggcgtgcat
     4081 ctttataaaa ggaatatccg ccagcggtgt tggagatgag accgaaatag gattcactgc
     4141 caaggtagtt gatccagggc agtggggtct caggagtatc aattacatat tcgcgatttg
     4201 tgtcatcaaa atgtccgtaa cgcatataac ctctttaatt tgtttggctt attttttcaa
     4261 agctgggttt caacgccggg tatagctcac gataaagcgg atacgaccnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat caaggtgaag ctatctttca aaccaaatgc
     4441 cacgccttct aacacggcac gagtcatatg tgcgcgcgtg tgacgaagcg ttagcccaac
     4501 gaaggaaccg cgtgcgagtg ggtctgggta aggtgtgcgt tcaccgctga gataaggaag
     4561 gaacaacaaa ccttccgaac cagcgggcgc actctcggct tctttcacca attcatcaaa
     4621 gcttacattg ggcgcgagag tgtcgcgata ccactgcaaa cttcccgccg cagaaagcat
     4681 cacacccata aaatgccaca tacccggcac agcatgacag aacgcatgca agcgaccttc
     4741 aggttcgatc aatgcggaag gcgttgttgc aaagaccaca ccgctggtgc caaccgtcag
     4801 accaacaatg cccggttcta ccgcccccac acccacagcg ccggctgcct ggtcaccacc
     4861 gcctgcggcg acaggtgtgc ctgctttcaa accagtgaga cgtgctgctt cttcagtcac
     4921 tttgcctgtg aacgctgtgc cttcaaacgt gcgcggcatc cattcgcgcg gaatgcccaa
     4981 tgccgccaaa acttcatcgg accagtcgcg tactttcaag tcaaacaaaa cggtgccagc
     5041 gccatcggct ttgtccatgg cgtattcgcc tgtcaaacaa aagcgaatgt aatccttggg
     5101 caacaacaca tgttttgctc ttgcatacac actgggctcg ttctctttca cccataaaat
     5161 ttttggtgct gtaaaaccgg tcaacgctac attgccggtg atttgaataa atttttcctt
     5221 gccgatcact tgatgaattt catcgcactg actttgcgtg cgttggtcat tccacaagat
     5281 ggcaggacgc aacacactcc cgttttcatc caagaggact aagccatgca tttgacccgt
     5341 cagcccgaca gcgacgactc tttctccgct cacgcctgcc ttttccaaca cagacttgat
     5401 actcgctgca actgcatccc accactcgcg cgggttttgc tccgaccata acggcttggg
     5461 tgtttgcaac gtatgcgatg ccgcagcgac cgcaatcacc acgccatttg agtcgatcaa
     5521 taatgccttg ctagatgtgg tggacgtgtc aattccgaga aaatagttca taagctttac
     5581 tccaattcca ggcgttctac caaggcttga acatcctgtt cattttgtgg gcgttcaata
     5641 ccttggtaca tttcccatgt ctctacatga gtagcttctg cgccgggttc tatggttgtg
     5701 atgggagata aggtctcaag ctcaaggatc tcatggttgc aataactttc gcttgaacta
     5761 ccatagtcaa aatactcggc atctgcctca tatttggcgc gttttacgaa caaaactcca
     5821 ttccaccaat aggcttgcca accgcgcggg ttcgagaagc caactttgaa cgctccctgc
     5881 atgttggcgt tgatcagtat atagttcttt ccgaggtgca cagccggttc agatggatcg
     5941 gtatagggcc agaaggtgag cctgcggttt ggcaatacac ctgtattttc aaccgtttgc
     6001 ggcaagatgc caagtccgcc cggcttgaat tgcgtgattg cccataaggc ggtggttgca
     6061 gaccaaagtc ctttgttggt taaacgatgt gtgatgctga gagatgcgca atccccctgc
     6121 atttgaatat cgaatgattt ttgtaaaccg gtttttgcct ctacctcttg aacgatgtgt
     6181 aatgtgtttt cgatctgtgt gatatctact ggcgagtcat cgggtaagta ggtgcggctt
     6241 ggcacttcgg gggcatgcca taggcgatgc cccccataaa agtgataaa
//
LOCUS       DAOQFT010000063         6222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6253_length_6222_cov_6.898006, whole genome shotgun sequence.
ACCESSION   DAOQFT010000063 DAOQFT010000000
VERSION     DAOQFT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6253_length_6222_cov_6.898006"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLR93_02650"
     CDS             complement(<1..452)
                     /locus_tag="PLR93_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25888.1"
                     /translation="MAWEPKIMRKVRIRNSQKKGVLGKLLTAVAEAGGNVGSINLLTE
                     TSRHVVRDITITAEDEKSLAAVLAAMETNEGTKILEVRDEVLELHQKGKIAIRSRYPI
                     ESLTTLRRVYTPGVAEVCNRIAKDPSQARLYTSISHMVAIVTDGTAVLG"
     gene            complement(546..1262)
                     /locus_tag="PLR93_02655"
     CDS             complement(546..1262)
                     /locus_tag="PLR93_02655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNH25889.1"
                     /translation="MPNKNIKAVLFDLDGTLRLNQPASRDVFAECVQGMGLVPSEEDQ
                     TRAERWEHLYFASSLEIQEDSKIFKDDRKAFWVNFTKRRLVALGVHKPQAEELASHVS
                     DCMGMNYKPQPYLPDSIHELLKHLQRTGYVLGVVSNREEPFHSELEELGLKEYFHFSL
                     AGGEVQSFKPHPRIFERALELSGTAASETMYVGDNYFADVVGALRSGLLPVLFDPTGL
                     FPDAECDVIRSYAEFYKLLM"
     gene            1418..2497
                     /locus_tag="PLR93_02660"
     CDS             1418..2497
                     /locus_tag="PLR93_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNH25890.1"
                     /translation="MSFEFILRIIGMIVLAVSGGFLGYDLSRFTPEDAVRTVLVFSLV
                     GALTGLVLTPYFTTRPARAIRSLLGRMTAESLFAGLTGLIVGLLIAALLAFPLSMLPQ
                     PFSQILPFIGVLIFSYFGVALFVMRQGDIMGLLSALSGRGEGGSGSSWSNLNRNILLD
                     TSVIIDGRVSDIAKTGFLPGTLLIPRFVLNELQYIADSPDGMRRQRGRRGMEVLAELQ
                     KLPNILVRISDINVDGVREVDDKLVVLGKQLKSPILTNDYNLNRVAELQGVTVLNINE
                     LANAVKSVVLPGELLRINIMQEGKEHGQGVGYMDDGTMVVVENGRDFIGEYMEVNITK
                     VLQTAAGRMIFGRVDEENSKKSKNK"
     gene            2646..4034
                     /gene="cysS"
                     /locus_tag="PLR93_02665"
     CDS             2646..4034
                     /gene="cysS"
                     /locus_tag="PLR93_02665"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNH25891.1"
                     /translation="MLKIYNTLTRKTEEFQTLEPNKVKMYVCGVTVYNDAHVGHAMSA
                     IVFDIVRRYLEYRGYAVKHVMNYTDVDDKIINRANQLGEDPLKLSARYIADYAADLKS
                     LNVLPATSNPQVSTTMPLIIQFIEGLIQKGHAYAAENGDVYFRVTSDDDYGRLSGRKL
                     EDMQAGARIEVGEQKEHPMDFALWKAAKPGEIFWESPWGKGRPGWHIECSAMNLAELG
                     EQIDIHGGGNDLIFPHHENEIAQSESYTGKEFSRYWIHNGMLQLGGEKMSKSLGNIIS
                     IKEFLSKRSSDVMRMLVLNGTYRAPLMFNDETLDAAERNVERLKSALKPASPSASGLS
                     ADTLSVSVESSKTNFTEAMDNDFNTAGAVAALFELSKSINTARDNGATADQLKPAQET
                     FKQLAGVLGLKLEEKKGSSEDEAQVEALLAERTQARKDKNWKRSDDIRDQLKAMGVTI
                     EDSKDGTTWKWS"
     gene            4163..4384
                     /locus_tag="PLR93_02670"
     CDS             4163..4384
                     /locus_tag="PLR93_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNH25892.1"
                     /translation="MRRMFGFMIGIFTGALVGSVIALLLAPESGENLRGQLRNRGQNF
                     LNDVRHSADARRIELRNHLESLRSSREEF"
     gene            complement(4381..5547)
                     /locus_tag="PLR93_02675"
     CDS             complement(4381..5547)
                     /locus_tag="PLR93_02675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH25893.1"
                     /translation="MRLLFVTDARSPISKNWIRHFIERGDEVFIASTFEWDVEGIPSE
                     RTEFTPVAFSSAKKRTSAPSTASSRTLSLRTKIRQWLGPVTIPAAAFKLRAFIREVQP
                     DIVHAMRIPYEGMLTAEALQGLKARPTFLASIWGNDFTLHAPSTPLIRYYTRKVMNSV
                     DGLHADVERDVKLAHKWGLSAEKPTLVTPGNGGIRSDVFYPPKELVKSPIIINPRGVR
                     PYVRNDSFFKAIPLVLAKYPDAKFLCVGMQNEPQAMRWMQEYGIENSVELLPAFLHEK
                     MGEVFRGAQIVVSPSIHDGTPNTLIEAMACGCFPIAGDLESIREWISHGQNGLLFDSN
                     NPQSIADAMLLGLEREDLRTEAAGLNKKIISARAEFSTNMNKVEKFYSVLRGTN"
     gene            5612..5986
                     /locus_tag="PLR93_02680"
     CDS             5612..5986
                     /locus_tag="PLR93_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH25894.1"
                     /translation="MSAGRILIVEDNMDTYELVRFILEKNGFETFLAMNGRDGVNAAT
                     KQRPDLIIMDLSMPEMDGWTAMRLIKSDPAISSIPMIALTAHALPSDRKRAFDAGCDE
                     YITKPMDLIDLLDTVKSWIQKV"
     gene            6071..>6222
                     /locus_tag="PLR93_02685"
     CDS             6071..>6222
                     /locus_tag="PLR93_02685"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNH25895.1"
                     /translation="MKQICVVGVGYVGLVTAACFADLGNRVVALDVNEERVANLKKGI
                     MPIYEPG"
BASE COUNT         1635 a         1578 c         1432 g         1577 t
ORIGIN      
        1 cctagcacgg ctgtcccatc ggtaacaatc gccaccatgt ggctaatgct cgtatacagc
       61 cgagcttgcg atgggtcttt ggcaattcga ttacaaacct ctgcaacgcc aggggtatac
      121 acgcggcgca gagtggtcaa tgattcaatc gggtatcgcg agcggatggc gatcttgcct
      181 ttttggtgca actccaacac ctcatcacgc acttcgagaa tctttgtccc ctcgttggtt
      241 tccatcgccg ccaagactgc agctagagat ttttcatcct ctgcggtgat ggtaatgtcc
      301 cgaacaacat gccgggaggt ttccgtcaac aggttgatac ttccgacatt tccacccgcc
      361 tcagcgacag ccgttaataa tttacccaac accccttttt tctgtgaatt tcgaatgcgc
      421 accttgcgca tgatcttcgg ttcccacgcc atggttatct cctttttggt tatgctcatt
      481 ttaacgcgaa taccgcgaag tcgcttcgcg gtattggaac agacaacttt ataaagaaat
      541 cgagtttaca tcaataattt gtaaaactca gcatatgagc ggatcacgtc acattcagca
      601 tcagggaaga gtccggttgg gtcgaagagc acaggcaaaa gtccagaacg aagcgcaccg
      661 accacatctg caaaatagtt atcgcctaca tacatcgttt cgctggcagc tgtccctgag
      721 agttccaatg cgcgttcaaa gatacgggga tgcggcttga aagattggac ttctccacct
      781 gccagtgaaa aatggaaata ttctttcaat cctaattctt caagttcaga gtgaaatggt
      841 tcttcgcggt tcgataccac accgagcaca taacctgttc tttgaagatg cttcaacaac
      901 tcgtggatac tgtcaggcag ataaggttgc ggtttgtaat tcatgcccat gcaatcagaa
      961 acatgcgaag caagttcttc ggcttgtggt ttatgaacgc caagcgcaac caaacgccgc
     1021 ttggtaaagt tcacccaaaa tgctttacgg tcatccttaa agattttaga gtcttcttga
     1081 atctcaagcg agcttgcaaa atacaaatgt tcccaacgtt cggctcgggt ttgatcttct
     1141 tcagatggga ctagtcccat cccttggaca cattccgcaa acacatctcg tgatgctggt
     1201 tgattgaggc gcagggttcc atcgaggtcg aaaagaacag ctttgatgtt tttatttggc
     1261 attattagca gagtctcatg tagtttttga aaatcagagg ggaaatctaa aaggattata
     1321 tcaattttat taaccattct tactcgacaa ttgacggagg atgccagcca tgctatgatg
     1381 acatggttat tattcttttt tttattcagg agttatgatg agttttgagt ttattcttcg
     1441 tattattggc atgatcgtct tagctgtttc gggtggtttt ttgggatatg acctttcgcg
     1501 gttcactcct gaagatgcag ttcgcactgt gcttgttttc agcttggttg gagctttaac
     1561 tggtttggta cttaccccgt actttacaac ccgccctgca cgcgccatcc gatctctgtt
     1621 gggacgtatg actgcagaaa gcctatttgc tggcttgaca ggactcattg ttggtttgtt
     1681 gattgccgcg ttgttggcat ttccgctttc catgttgccc caacccttca gccagatcct
     1741 tcctttcatt ggcgtattga tcttctcgta tttcggtgta gctctctttg tgatgcgtca
     1801 gggagatatt atgggattgc tgagcgccct aagcggacgt ggcgaaggcg gaagcggatc
     1861 ttcatggagc aacctcaacc gcaatatttt gctcgatacc agcgtcatca ttgatggacg
     1921 tgtttctgat attgccaaga ccggttttct gcccggcaca ttgcttattc cgcgcttcgt
     1981 gctgaacgaa ctgcaatata tcgcagactc acctgatggc atgcgccgtc aacgtggtcg
     2041 ccgtggcatg gaagtacttg ctgaattgca gaaactgcct aacatccttg tgcgcatctc
     2101 agatatcaat gtagatggcg tccgtgaagt ggacgacaaa ttggttgtac tcggcaagca
     2161 actcaagagt cccattctca ccaacgatta caacctcaat cgcgtcgctg aattacaagg
     2221 tgtgaccgtc ctcaatatca acgagcttgc caatgcggtc aaatcggtag tgttgcctgg
     2281 cgaactcctg cgcatcaata ttatgcagga aggcaaagaa cacggacagg gcgtaggcta
     2341 catggacgat ggtacgatgg tcgtcgtcga aaatggacgt gatttcattg gcgaatacat
     2401 ggaagtgaac atcaccaagg tccttcagac cgcagccggg cgtatgatct tcgggcgtgt
     2461 agacgaagag aatagcaaaa aatctaaaaa caagtaataa gcaacaaggg acgagtagtc
     2521 agtgaacagg acgtgtgaag cgtcctgttt ttttgtccca atgcggtaaa atacaaccgt
     2581 cttaaaaatt cctttgtgat ctcaagccct cgaataaggg catggcactt ggtggtaaaa
     2641 caaacatgtt gaaaatttac aacactctca cccgcaaaac cgaagaattc caaaccctcg
     2701 aaccgaataa agtcaaaatg tatgtttgtg gcgtcacggt ctataacgac gctcatgttg
     2761 ggcatgccat gtctgccatc gtgttcgata tcgtccgccg ctacctcgaa tatcgcggat
     2821 acgccgtcaa acacgtgatg aactacaccg acgtagacga caaaatcatc aaccgcgcca
     2881 accaacttgg tgaagaccct ctcaaacttt cggcgcgtta tattgctgat tatgccgccg
     2941 acctcaagag tctcaatgtt ctgcctgcca cgtccaatcc gcaggtcagc accaccatgc
     3001 cgctcatcat ccaattcatc gaagggctga tccaaaaagg tcacgcctac gccgcagaaa
     3061 atggcgatgt ctatttccgc gtcaccagcg acgatgatta cggtcgcctc tcgggtcgca
     3121 aacttgaaga catgcaagcc ggcgcacgca tcgaagttgg cgaacaaaaa gaacacccga
     3181 tggatttcgc gctctggaaa gccgccaagc ccggcgaaat tttctgggaa agcccatggg
     3241 gcaagggccg ccccggttgg catatcgaat gctctgctat gaacctcgcc gaacttggcg
     3301 aacagatcga catccacggc ggcggaaacg acctcatctt cccccaccac gaaaacgaaa
     3361 ttgcccaaag cgaaagctac accggcaaag aattctcgcg ctattggatc cacaacggca
     3421 tgctgcaact cggcggcgag aaaatgtcca agtctttggg caatatcatc agcatcaaag
     3481 aattcctctc caagcgttct tccgatgtca tgcgcatgtt ggtcttgaac ggaacctatc
     3541 gcgcccctct gatgttcaac gacgaaacgc tggatgccgc tgaacgcaac gtcgaacgcc
     3601 tcaaatccgc gctcaagcct gcctccccct ccgccagcgg actctccgct gataccttgt
     3661 ccgtttcggt cgaatcctca aaaaccaact tcaccgaagc catggacaat gacttcaaca
     3721 ccgccggagc cgtcgctgca ttattcgaac tctccaagtc catcaacacc gcccgcgata
     3781 acggcgcgac cgccgatcaa ctcaaacccg cgcaagagac tttcaaacaa ttggctggcg
     3841 tactcggttt aaaactcgaa gagaaaaaag gctccagcga agacgaagct caagtggaag
     3901 ccctcctcgc cgaacgcacc caagcccgca aagacaaaaa ctggaagcgc tccgacgata
     3961 tccgcgacca gctaaaagcg atgggtgtca ccatcgaaga cagcaaagac ggcactacct
     4021 ggaagtggag ctagtttcgt agtgtgacag tcaatcagtt gaatagtaaa acttccgaga
     4081 aatgtttctc ggaagttttt atttgtaaca actttcagat gtataattca attatcaaaa
     4141 cgaattttca aggagaaatt ccatgcgtag aatgtttggc tttatgatcg gcatcttcac
     4201 cggcgcgtta gtcggctctg tcattgcact tcttctcgcc cctgaatccg gtgaaaacct
     4261 gcgcggacaa ctccgtaacc gcgggcaaaa cttcctcaac gatgtgcgtc actcggcaga
     4321 tgcccgccgc attgaattac gcaatcactt agagtctctt cgctcctcaa gagaagaatt
     4381 ctagttcgta cctcttaata cagaatagaa cttctccact ttattcatat ttgtactaaa
     4441 ctctgcacgt gcagagataa ttttcttatt cagacctgcg gcttcggtgc gcaggtcttc
     4501 tctttctaga cctaataaca ttgcatcagc tatcgattgc gggttgtttg agtcaaacaa
     4561 caaaccgttt tgcccatgtg aaatccactc acgaatggat tccaggtcgc ctgctatggg
     4621 gaaacatcca cacgccatgg cttcgatcag cgtgtttggc gtcccatcgt gaatactggg
     4681 ggaaaccacg atctgtgcac cacgaaacac ctctcccatc ttttcatgca agaaggcagg
     4741 caacagctcc acagaatttt caattccata ctcttgcatc cacctcatgg cttgtggctc
     4801 attttgcata cctacacata agaacttcgc atcaggatat tttgccagca ccaagggtat
     4861 cgccttaaaa aacgaatcat tccgcacata gggtcttact ccacgagggt tgatgatgat
     4921 tgggcttttc accaactctt ttggcggata aaaaacatcg cttctaattc cgccgttacc
     4981 gggtgtcacc aacgtaggtt tctctgcact caagccccat ttatgtgcca gttttacatc
     5041 ccgctctaca tctgcatgca aaccatctac agaattcatc accttgcgag tgtaatacct
     5101 aatcagtggt gtagatgggg catgcagggt aaagtcattg ccccaaatgg aagcgagaaa
     5161 tgtagggcga gccttcagcc cttgtagggc ttcggcggtc aacatacctt catacggaat
     5221 gcgcatggca tgaacaatat ctggctggac ttctctaatg aaagcccgca atttgaacgc
     5281 ggcagcggga atggtaacag gaccaagcca ctgtcgaatt ttggtgcgca ggctcagggt
     5341 ccgggaagag gcggtggagg gagcagaggt acgtttttta gcagagctaa atgccacagg
     5401 ggtgaattca gtccgttcgg aggggatacc ctccacatcc cattcaaagg tagaggcgat
     5461 aaaaacctcg tctccgcgct cgataaaatg acgaatccag ttttttgaga tgggggaacg
     5521 ggcatctgtg acaaatagca ggcgcataag gtgattatac attgtaaaaa tattgggcta
     5581 tcgcaaccta ctttgtagta tatttaagcg catgagcgca ggacgcattc ttattgttga
     5641 agataatatg gatacctacg agctggtacg ttttattctg gagaagaacg gttttgaaac
     5701 cttccttgct atgaatggac gagacggcgt caatgctgcc actaaacaac gcccagactt
     5761 gatcattatg gacctttcca tgcctgaaat ggatggttgg accgccatgc ggttgatcaa
     5821 aagcgaccct gccatatctt ccattcctat gatcgcccta actgctcatg ccctgcccag
     5881 tgaccgcaaa cgtgcgttcg atgcaggctg cgatgaatac atcaccaaac ccatggacct
     5941 gatcgatttg ttggataccg ttaagagttg gattcaaaaa gtataaatca ggctagggaa
     6001 aataaatggc ttatagcaat atacaaagtc tcatattaaa gagccaaatc ttcaaaaaag
     6061 gaaatacacc atgaaacaaa tttgtgtcgt cggcgtgggc tacgtcggtc tcgtgaccgc
     6121 tgcctgtttt gccgatttag gtaaccgcgt ggttgccttg gatgtcaatg aagaacgcgt
     6181 tgccaatctc aaaaagggca ttatgcccat ttacgaaccc gg
//
LOCUS       DAOQFT010000064         6179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6321_length_6179_cov_5.572012, whole genome shotgun sequence.
ACCESSION   DAOQFT010000064 DAOQFT010000000
VERSION     DAOQFT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6321_length_6179_cov_5.572012"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="PLR93_02690"
     CDS             complement(<1..1800)
                     /locus_tag="PLR93_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH25896.1"
                     /translation="MNTTLEIINAPLDALILYQYKNGMKKLRLLLGLWLATSLVACST
                     LKNFPTIPPGWTVTPSTSPTPEATFTPTITPTPLPVARVSEGDKAFFNGDFDAALLHY
                     QVAIQDSPDPLVRAAAEWGQAKIYYAQGRYNETLTSLQTLISEYPQAPQHPQAYFLQG
                     FVYYKLENYPAAADSWQTYLVLRPSVLDAYVQELRGDALFNAKDFAGALASYTTAITA
                     PSLGDDTTLDLKVASVQTQLGNYEEAITLYDGIIGRATSDYTKAQALYEAGTVNLTQG
                     QTAEANERFRYAIENYPLSYYSYLSLVALLDAGGTVSDLDRGLVDYFSGQYAVGIAAF
                     DRYLQSNPADNDGTAYYYRALCKNALGQYDEAIADYQNFISNFSAHPRWGDAWGEKAF
                     IEWAQKGDYTTGAQTLLEFINLAPTSPQAAEYLMSAARIYERDAQYDKAIETWSRVVI
                     EYPGSQQASYAAFLSGIVEYRRGNHQASLDLFKRSLALSVSVEDKSRALLWIGKANQI
                     LGNQGPAEDAWREGQNLNPGGYYSERARDLLVGQAPFATGSAQNYQVDYARERADADT
                     WMRLTFNLGNDVDLSNLGSLASDARIIRGKEYWD"
     gene            1857..3008
                     /locus_tag="PLR93_02695"
     CDS             1857..3008
                     /locus_tag="PLR93_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNH25897.1"
                     /translation="MNTHHKIHRWFRLILSSVLLIAVTACAAGSAPASTDELVTANGF
                     VCPEPSPRVEFESDTINIFTWTEYVPADMIDCFGMVYDITVNVDYFSSNEELYSKVSL
                     GEDVNPYDVIHPSDYMIAVLMREGLLQELDPARLPNLANLDSGLVTAYGEATSYVVPY
                     QMGTQAIIYNSDTVETPPTSWADLWNPEYEGRIVSVDDSRVVIGAALLTLGYDVNDTD
                     PDHLEEAKQKLLELMPNIRVFDSDSPKTPLVAGDVDLGIVWNGEAFLTASEAPQFKYV
                     FPEEGSIIFYDGMGIPPSATHADAAYAWFNYMLQGDVNWLTLVDYPYTNPNKAALDYA
                     KENQPDIYEAYISSSITNTPADEFAKGHDVEDIGEALLIYDEIWTEIKQ"
     assembly_gap    3142..3241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3242..3892
                     /locus_tag="PLR93_02700"
     CDS             <3242..3892
                     /locus_tag="PLR93_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH25898.1"
                     /translation="FATTDHASAQIASFYPGAMGYSATQSLKELDKKPDLVIVSPSSP
                     DAMMKFPAEARELGIPYLYDPSQQVLRLEGPELARDMEGAYFLFCNDYEFGLISKKTG
                     WSLDQILNHVKVLVITRGKDGADLYSDGTSVHIPTVPEDEIVDPTGVGDAFRGGFLAG
                     YSHGFDWKLCGEIGSLSAVYCLEQRGTQNHSYTREDFVKRFRKHFDDGGRLDVLLK"
     gene            4089..5420
                     /gene="ahcY"
                     /locus_tag="PLR93_02705"
     CDS             4089..5420
                     /gene="ahcY"
                     /locus_tag="PLR93_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNH25899.1"
                     /translation="MSNYDIKDLQLAEGGRRRIDWAEREMPVLRSIRERFKKEKPLKG
                     MRLSCCLHVTTETANLMITLQEGGADIVLTASNPLSTQDDVAAALVNNYEIPVFAIKG
                     EDKVTYFKHIRAALEHKPHMTQDDGADLVSSLFFIEMGRFEKLEPSLASWAKGLSEEE
                     RKQMLKNVIGGTEETTTGVIRLKAMEADGVLKFPVIAVNDALTKHMFDNRYGTGQSTI
                     DGIIRATNVLLAGKTFVVAGYGWCGRGLASRARGMGAHVIVTETDPMKALEAVMDGFQ
                     VMSMLDAAKIGDIFCTVTGDINVLDKHHFEVMKDGALVSNSGHFNVEINIPALAAMSK
                     GEPNLVRPFVEQYTTKDGRKINILGEGRLINLASAEGHPASVMDMSFANQALSAEYMA
                     KNADKLEKKVYSVPAEIDNEIARLKLEAMGVKIDKLTDEQLHYLNSWEEGT"
     gene            complement(5484..6014)
                     /locus_tag="PLR93_02710"
     CDS             complement(5484..6014)
                     /locus_tag="PLR93_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25900.1"
                     /translation="MPNDANQRALDFANAVTQTVNYRDSNQSIQEKIRDDHFVSKLGE
                     EAVRILFQSRECLVEGPDYGVYEARRKSWAADLKINGLDVAVKTQRRSAANRYGLSWT
                     FQDSPERHDPILDMPDAWVCLVVFEDLKKEPECLVYPLRKIKQLIFEAPRLSKLVGKK
                     QAVYLETLIKNGVIEK"
BASE COUNT         1514 a         1417 c         1490 g         1658 t
ORIGIN      
        1 atcccaatat tctttgccac ggataatgcg tgcgtccgaa gccaacgaac cgaggttgct
       61 caagtctaca tcgttgccaa ggttgaatgt taaccgcatc caggtatcgg catcggctcg
      121 ctctctggcg tagtccactt ggtaattttg ggcgctgcct gttgcaaatg gagcttgccc
      181 taccaatagg tctcgcgcac gttcactgta atagccgcca gggtttaggt tttgtccttc
      241 acgccacgcg tcttcagctg gaccttgatt tcctaaaatt tgattggctt tgccgatcca
      301 taacagggca cggcttttat cttcaacaga aactgaaagt gccaggctgc gtttaaaaag
      361 gtctaacgat gcttgatgat tgccgcgccg gtattccacg atgccagata aaaacgctgc
      421 atatgaagct tgttgcgaac cggggtattc aatgacaaca cgagaccatg tttcaatcgc
      481 tttatcatac tgggcatctc gttcgtaaat gcgtgcggca ctcataagat actcagctgc
      541 ttgtggggaa gtaggggcga ggttgataaa ctccaagagc gtttgagcgc cggtggtgta
      601 atctcctttt tgtgcccatt cgataaacgc cttttcaccc catgcatctc cccagcgtgg
      661 atgcgcagag aaattcgaaa tgaagttctg ataatctgct atcgcttcat cgtactgccc
      721 caatgcgttt ttacaaagcg cacggtaata atatgccgta ccgtcattat ctgcagggtt
      781 actttgtaag tagcgatcga atgcggcgat gcctactgcg tattgacctg aaaaataatc
      841 tacgagccca cggtctaggt cactgactgt tccaccggca tcaagcaaag cgacaaggct
      901 aagataggaa taataggaaa gtggataatt ttctatagcg tatcggaaac gttcattcgc
      961 ctcggcggtc tgaccttgag tgagattcac cgtcccagct tcgtataacg cttgagcctt
     1021 ggtgtagtca ctggtggctc gtccaatgat tccgtcgtat aacgtaattg cttcttcata
     1081 attgccgagt tgtgtttgta cggatgcgac ttttaagtcc aaggtggtgt catcgccgag
     1141 agatggggcg gtgattgcag tggtgtaaga cgccaacgct ccggcaaaat cttttgcatt
     1201 gaagagagca tcgcctcgta gttcttgtac gtaggcatct aatacgcttg ggcgcaagac
     1261 taaataagtt tgccaggaat cagccgccgc agggtagttt tcaagcttgt aatagacaaa
     1321 gccttgtaga aaataagctt gtgggtgttg aggagcttga ggatattcac taataagggt
     1381 ttgaagggat gtgagggttt cgttgtagcg cccttgagcg taatatattt tggcttgacc
     1441 ccattctgct gcggcacgca ccaaggggtc tggtgaatct tgtatggcaa cttgatagtg
     1501 taaaagggct gcgtcaaaat caccgttgaa gaaggcttta tccccttcgc tgactcgggc
     1561 aacgggtaaa ggcgtaggcg taatggtggg agtaaaggtc gcttcgggag tgggggatgt
     1621 gctgggagta accgtccagc cgggggggat ggtagggaaa tttttgagcg tagaacaagc
     1681 tacgagagat gtggcaagcc aaagtccgag aaggaggcgc agttttttca tcccgttttt
     1741 atactggtaa aggatgagtg cgtcaagcgg ggcatttatg atttctaatg ttgtattcac
     1801 gtttagcttg cactataatc tctttgttac attattttat aaccctaagg agaattatga
     1861 acacacatca caaaatccat cgttggtttc ggttgatttt gtcgagcgtg ctgctgatcg
     1921 ccgtgacagc ctgcgctgct gggtctgccc ccgcctcgac ggatgaattg gtcactgcca
     1981 atggctttgt ttgcccagag ccgagtccac gcgtggaatt tgaaagtgat acgattaata
     2041 tattcacttg gacggaatat gtgccagccg atatgatcga ttgttttggc atggtgtatg
     2101 acattaccgt taacgttgat tatttttctt caaatgaaga gctgtattcg aaagtttcgc
     2161 ttggcgaaga tgtaaaccct tatgatgtaa ttcaccccag cgattacatg atcgccgttt
     2221 tgatgcgtga agggctactg caagaactgg accctgcgcg tttgcctaat ttggcaaacc
     2281 ttgactcggg tcttgtaacg gcttatggcg aagcgactag ttatgttgtg ccgtatcaga
     2341 tggggacaca ggcgattatc tataacagtg acactgtgga aactccgcca acttcttggg
     2401 ctgacctttg gaaccctgaa tatgaaggtc gcatcgtgtc tgtggatgac agccgagtgg
     2461 tgattggtgc cgctttgttg actttgggtt acgatgtcaa cgataccgac ccagaccatt
     2521 tagaagaagc taaacagaag ttgttggaac ttatgccgaa cattcgtgtg ttcgatagcg
     2581 atagccccaa aactccactg gtggcaggcg atgtagattt gggaattgtt tggaatggtg
     2641 aagcgttcct gacggcttct gaagctccgc agttcaaata cgttttccca gaagaagggt
     2701 cgatcatctt ttatgatggt atggggattc cgccttcagc gactcatgcc gatgcagcat
     2761 atgcctggtt caactatatg ttacagggcg atgtcaattg gctgaccttg gtcgattatc
     2821 cgtacaccaa ccccaacaaa gctgcgttag attatgccaa agaaaaccag ccagacattt
     2881 atgaagctta tatctcttca tccatcacca acaccccggc agatgagttt gcaaaagggc
     2941 atgatgttga ggatataggc gaagccttgc tgatatacga cgaaatttgg acggaaatta
     3001 agcaataaag taggcgaatt gcacgggtta tttttgtaag ctcaaaaact ttacattcat
     3061 aaaaccccgt gataaacttt tgcccgttcg atatcttatg gcgcactcaa ccgagtgcgc
     3121 catttactgc gagaaggaaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nttcgcgaca acggatcatg cctccgctca gattgcttcg ttctaccccg gagccatggg
     3301 ctactctgcg actcaatcgc tcaaagagct tgataaaaaa ccagacctcg ttattgtctc
     3361 tcccagttcg ccagatgcga tgatgaaatt ccctgccgag gcacgtgaat tgggtattcc
     3421 atatttgtat gaccccagcc agcaagtctt gcgccttgaa ggaccagaac ttgctcgcga
     3481 tatggaaggg gcatatttct tgttctgtaa tgactacgaa tttggcttga tctccaagaa
     3541 gaccggctgg agcctcgacc agatcctcaa ccatgttaaa gtcttggtga ttacgcgcgg
     3601 caaagacggc gcagacctct actcagatgg cacttcggtt cacattccca ccgtgcctga
     3661 agatgaaatt gtagacccca caggtgtggg ggatgccttc cgtggcgggt tcctcgccgg
     3721 gtattctcac ggctttgatt ggaagttgtg tggtgagatc ggctcccttt ctgcggtcta
     3781 ctgccttgaa caacgcggca cccagaacca ttcttataca cgcgaagatt tcgtcaaacg
     3841 tttccgcaag catttcgatg atggcggcag actggacgtc ttattaaagt aaaatctatt
     3901 tagatttgtt ggttaagctg gcggtgttcg cagaatgttg ccgtacatac gcattgctcc
     3961 gcttgaaagc aatgtcaact gttgagacca acctaaatta aattattagt ccgtgatttg
     4021 aaccctttta tgcggacaca aatgggtttc caaatcacat tccttgtata aagacttaag
     4081 gagtatttat gagcaactac gatattaaag atcttcaatt agcagagggc ggacgccgtc
     4141 gcattgattg ggcagaacgc gaaatgcctg tgctgcgttc catccgcgaa cgcttcaaga
     4201 aggaaaagcc tctcaagggt atgcgtctat cttgctgttt gcatgtgacc accgaaactg
     4261 caaacctgat gatcaccctt caggaaggcg gcgcggacat cgtcctcact gcctccaatc
     4321 cgctttccac tcaagatgat gtggctgcgg cgttggtcaa caactatgaa atccctgtct
     4381 ttgctatcaa gggcgaagat aaggtcactt acttcaagca catccgcgct gcgttggaac
     4441 acaaacccca catgacccag gacgatggcg ccgacctcgt ttcctccctt ttcttcatcg
     4501 aaatgggacg tttcgagaaa ctcgaacctt ctctcgcttc ttgggcgaag ggtttgagcg
     4561 aagaagaacg caagcagatg ctcaagaacg ttatcggtgg tactgaagaa accaccaccg
     4621 gcgtgatccg cttgaaggcg atggaagccg atggcgtgct caagttcccg gtcattgctg
     4681 tgaatgatgc tctcaccaag cacatgttcg ataaccgcta tggaaccggt cagtccacca
     4741 ttgacggtat catccgcgcc accaacgttt tgttggctgg taagaccttt gttgtcgctg
     4801 gctatggctg gtgtggacgt ggtcttgctt cccgcgctcg tggtatgggt gctcatgtga
     4861 tcgtcaccga aaccgacccg atgaaggcgc tcgaagcagt catggacggc ttccaggtta
     4921 tgtctatgct ggatgcagca aagattggcg atatcttctg caccgttacc ggtgatatca
     4981 atgtgcttga taagcatcac tttgaagtga tgaaagacgg cgctttggtt tccaactctg
     5041 gtcacttcaa tgtggaaatt aacatccctg ctttggctgc catgagcaag ggcgagccca
     5101 accttgtccg cccgtttgtg gaacagtaca ccaccaaaga cggtcgcaag atcaacatcc
     5161 tcggcgaagg tcgcttgatc aacctcgctt ctgctgaagg tcaccctgct tccgtaatgg
     5221 atatgtcctt cgcgaatcag gctctctctg ctgaatacat ggcgaagaat gccgataagc
     5281 tcgagaagaa ggtgtattct gttcctgccg agatcgacaa cgagattgct cgcctcaagc
     5341 tcgaagcgat gggcgtcaag atcgacaaac tcaccgacga acagctccac tacctcaact
     5401 cttgggaaga aggcacctaa gtcgatctac aatcatcgat tttggattaa tgattacagt
     5461 cccacgaatt tcgtgggact gttttatttt tcaattacac cgtttttgat aagtgtttct
     5521 aaataaacgg cttgtttttt tccaacaagc tttgagagcc gcggcgcttc aaaaatcagt
     5581 tgctttatct tcctcagtgg atataccaga cactctggtt ctttttttag atcttcaaaa
     5641 actactaaac atacccatgc atctggcata tctaaaattg gatcatgccg ttcaggcgag
     5701 tcttgaaatg tccatgaaag tccatagcga ttcgcagcgg agcgacgttg ggttttgacc
     5761 gccacatcca atccgttgat tttcaagtct gccgcccagg atttacgcct cgcctcatac
     5821 acaccgtaat ctggtccttc caccaagcat tccctactct gaaagagaat ccgcactgct
     5881 tcttcgccaa gtttagagac aaaatgatca tctcgtattt tttcttgaat cgattgattg
     5941 ctatccctat aattgacagt ttgtgtgaca gcgttggcaa aatctaatgc tcgctgattg
     6001 gcatcatttg gcacaagaat agtttgcgga acatgaaatg ttgacatcgt ttctgattat
     6061 acaaaataaa aagccttaca gaaaaacttc tgcaaggctt tttaatactt atcaactttc
     6121 tatgggcagg cgaatattac atctttaatt gcagccttgc ctgcagagtt gaaggcttc
//
LOCUS       DAOQFT010000065         6178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6323_length_6178_cov_5.191246, whole genome shotgun sequence.
ACCESSION   DAOQFT010000065 DAOQFT010000000
VERSION     DAOQFT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6323_length_6178_cov_5.191246"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLR93_02715"
     CDS             <1..937
                     /locus_tag="PLR93_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH25901.1"
                     /translation="KGIGVAMVMQGTAIPYLDMGGASIKMNDDGSFNLLVGATDLGTG
                     SDTVIAQMAAEVLGVPIEDMITYSSDTDFTPFDKGAYASSTTYISGTAAVKAANIVAE
                     RNRIRAATMLEVADPNSIQLKDRHAHAPNGKSVSLAEIALDTLHKNNQEQIMGVASYV
                     SPVSPPPFAAQFAEVTVDTETGQVTVDRLVMAVDSGVIVNPLTASGQIEGGMTQALGY
                     AVCEEMRYDDKGVAYERDLDRYHIFRADEMPELETIFVETFEPSHPFGVKAVAEIPMD
                     GVAPAVGNAILDAIGVNVDVNPTTPERVWKAIKRQ"
     gene            1069..>1529
                     /locus_tag="PLR93_02720"
     CDS             1069..>1529
                     /locus_tag="PLR93_02720"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNH25902.1"
                     /translation="MTSSELAHIIPYLASFLISIFISGYAWTKRQAQGALGFELYMVG
                     QTVWIAGFILEMISKELGDKIFWDQVQWFCTTLIFVAIPIFAIQFTEHRIYFPRRYFF
                     LSLIVPMGFSFLLATNSIHHLIYNNPSLVASTPFSELHYDFTFVIYLYGLYG"
     assembly_gap    1530..1629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1630..2595
                     /locus_tag="PLR93_02725"
     CDS             <1630..2595
                     /locus_tag="PLR93_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNH25903.1"
                     /translation="IARDRVFEALADPVVILDKNNIVIDINAAMLTLMDKREHEVIGN
                     PAKDVFNDFPIPIKLYTHVSYARTEAVFEISGKQIHYDLTIWPLYNTNKEMVGRVYVS
                     HDITALKELEAELRKLNVQLEDRVRDRTRELAEAYESMLEGLALALELRDKETEGHSR
                     RVTENAIKLAQKMGIAGSALEDIRSGAILHDIGKISIPDEILHKPGKLTLDERAVVEK
                     HPETAFKLLSRIPFLSQAIEIPYCHHEKWDGTGYPRKLAGEDIPLSARIFAVADVWDA
                     LSYDRPYNKAWTREQIIEYFNEQTGKHFDPAITKIFLSMVEKGEI"
     gene            2637..3980
                     /locus_tag="PLR93_02730"
     CDS             2637..3980
                     /locus_tag="PLR93_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNH25904.1"
                     /translation="MPDQIYVVGHINPDTDSIASAMGYAWLLRERDGLNTIAARAGAL
                     NAQTSWVLKTLGLESPFLLTDASPRFDSVMQRLDSLRPNAQLGSAWTLASRTGGLAPV
                     VNEDGTPYGIISGMSLFKYFSNILGPRPGDTTVREMMSAECKDAADTSVPKYQANAHI
                     RDSLNRILRDEIDDYWVVDESGRYLGIARQRNLLNPPRLKIILVDHNEPRQSIAALEE
                     AELLEILDHHRLGNPYTHQPIRFTVDVVGSTSTLVTEQTAEAGLSAPPAIAGALLSGL
                     LSDTLILTSPTTTDRDRVAAERLARWAFVGGSPLQGETIETYGKAVLSAGAGLSNRKP
                     EEVVSTDIKPFEGGGFKFAVAQAEVTDSMQLSEHLAPLTKALDDLRDKRGLDFALLLV
                     TDVVRGTSRLIVTSSAPPILNDLPYPPLPDGTRDAPGVVSRKKQLLPAVLGLLER"
     gene            4041..4916
                     /locus_tag="PLR93_02735"
     CDS             4041..4916
                     /locus_tag="PLR93_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH25905.1"
                     /translation="MPFPYLDETLLLNAETRKDLPGSFITLPSGVTHYEEGGDPKGQP
                     VVLVHGFSVPYFIFEPTFVFLCQQGFRVLRYDLLGRGFSDRPTTRYNASLFVQQIKEL
                     LDALNYKNICLAGLSMGGAVTSSFIDAYPDYVSKYVLIDPSGARAVSLSPFLKAVRMP
                     GFGELAIGLFGSESMLKGIASDLFSPELVREFQEKYKVQMQYQGFKRAILSTMRNGML
                     DSFIETYKRVGKLKKPTLLFWGKQDTTTPFEHSADICAVMPHAEFYAFDSCGHIPHYE
                     RASEVNRILLEFLRK"
     gene            4968..5462
                     /locus_tag="PLR93_02740"
     CDS             4968..5462
                     /locus_tag="PLR93_02740"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH25906.1"
                     /translation="MNKEYIQTLYRYNQWSNARILDTAAKLSPEQFLATSEHPHRYLR
                     GVLTHTLFAEWIWHKRWTGESPTTRLKPEDFPTFEALRTRWAEEESQLMQFVAQVTDE
                     KLNAPFTYLATDGTKYENILWESMAHVVNHGMQHRSEAAAILTELGVSPGDLDFILFT
                     RKKL"
     gene            5459..>6178
                     /locus_tag="PLR93_02745"
     CDS             5459..>6178
                     /locus_tag="PLR93_02745"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNH25907.1"
                     /translation="MTFNRAKNFLFRNRHNLAVFVLLNLACVACIVFVAARVVYTDSS
                     RHTGLIWNLFLAWIPFILAYFAHALSWKKVWVYLAIPFVAFLWLLFFPNAPYMLTDLQ
                     DLARGTGYEAPLWYDVIIVVWCSWTGTLLGVISLYLMQDIIARTFHRWLGWVFVFAIS
                     GLSSFGIYIGRFVRLNSWDILQNPAETAMEILGIVIDPSKRLAAFTLAYTFFFLFIYL
                     LLYSFSHMLQEQVIRAQSSPEQ"
BASE COUNT         1556 a         1494 c         1459 g         1569 t
ORIGIN      
        1 caaagggatc ggcgtggcga tggtcatgca agggacagct attccttacc tcgatatggg
       61 cggtgcatcc atcaagatga atgatgacgg ttcgttcaat cttttggttg gtgcaacaga
      121 cttgggcacc ggttcagaca cggtcattgc acaaatggcg gcagaagtat tgggtgtgcc
      181 catcgaagac atgatcacgt attcatcgga cacggatttc actccgttcg ataagggcgc
      241 atatgcttcg tctacgactt atatttccgg cacggcggcg gtgaaggcag cgaacatcgt
      301 ggcggagagg aatcgcatcc gcgcggcgac gatgttggaa gttgcagacc cgaactccat
      361 tcaactaaaa gaccgtcatg ctcatgcgcc gaacggaaag tctgtttcgt tggctgaaat
      421 cgcattggat accctgcaca aaaataatca agaacagatt atgggcgtgg caagttatgt
      481 ttcgcccgtt tcacctccgc cgtttgcagc acaatttgcc gaagtgaccg tggatacgga
      541 aacgggtcag gtcaccgtgg atcgtttggt gatggcagtg gactcaggtg tgatcgtgaa
      601 tccgcttacg gcttcgggac agatcgaagg cggtatgacg caggcacttg gatacgccgt
      661 ctgtgaagag atgcggtatg atgataaagg cgtcgcttat gaacgcgatt tggatcgata
      721 tcacatcttc cgtgccgatg agatgccgga gttggaaacc attttcgtcg agacctttga
      781 gccgagccat ccgttcggag tgaaagcagt ggcggaaatt ccgatggatg gagtagctcc
      841 tgccgttggg aatgccatct tggatgccat cggtgtcaat gtggacgtga accccaccac
      901 gccagagaga gtctggaagg cgattaagcg tcagtaagaa gaaacaagca caaacgatag
      961 aatgaggcgt gaggcagtat ttgtccgcgc ctcattttct gtaaaattac aaaaataatt
     1021 ttgtctcgta tatacaaaga cagcgagata taatcaacgc cacaatacat gaccagttca
     1081 gaacttgctc acatcattcc ctatcttgct tccttcttga tatccatatt catatcaggt
     1141 tatgcatgga ccaaaaggca ggcgcaaggg gctttagggt ttgaacttta catggtgggg
     1201 caaacagtat ggatcgctgg tttcattctt gaaatgataa gcaaggaact aggggataaa
     1261 attttttggg atcaagttca atggttttgt acaacgttga tttttgttgc tatcccaatc
     1321 tttgcgattc aattcacaga gcaccgcatt tatttcccta gaagatattt cttcctatcg
     1381 ctcattgtgc cgatgggttt ctcgtttctg ttagcaacaa atagcatcca ccatttgata
     1441 tacaacaatc cgtcgcttgt agcaagtacg cctttttcgg agcttcatta cgattttaca
     1501 tttgtgattt acctgtatgg gctttatggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnna tcgccagaga cagggtcttt gaagcattgg cagaccctgt ggttattctc
     1681 gataaaaata atattgttat tgatatcaac gccgccatgc ttaccttgat ggacaaaagg
     1741 gaacatgaag ttatcggaaa ccctgccaaa gacgttttca acgattttcc gattcctatc
     1801 aagctgtaca ctcatgtctc atatgcgagg acagaagctg ttttcgagat tagcggcaag
     1861 caaatccatt acgaccttac gatctggcct ttatacaaca ccaataagga aatggtcggg
     1921 cgagtttacg tttcgcatga tattacggca ctgaaagaat tggaagctga gttacgaaag
     1981 ttaaatgtgc agttggaaga ccgtgtccgc gaccggacgc gcgaattggc agaagcgtac
     2041 gaatccatgt tggaggggct cgcgctggcg ctcgaattaa gagacaaaga aactgaaggt
     2101 cactccaggc gtgtaacaga aaacgccatt aagcttgcac aaaaaatggg tatcgcaggt
     2161 agcgcgcttg aggacattcg cagtggcgcc attctgcacg atattggcaa gatcagcatt
     2221 ccagacgaaa ttttgcacaa acccgggaag ttgaccttgg atgaaagagc cgtagttgaa
     2281 aaacacccag aaacggcatt caagctttta tcgcgtattc cttttttaag tcaggcaatc
     2341 gaaattccat actgccacca tgaaaaatgg gatgggacgg ggtatccgcg taaactagcg
     2401 ggcgaagata ttccactttc ggcacgtatc tttgctgttg ccgatgtttg ggatgccctg
     2461 agctacgacc gcccttacaa caaagcctgg actcgtgaac agatcattga atatttcaac
     2521 gagcaaaccg gcaaacattt cgaccctgcg atcacaaaaa tatttctctc catggttgaa
     2581 aaaggcgaga tataatccgt gccgcaaaat aaaatacgta aagaaagatg taacaaatgc
     2641 cagatcagat atatgtagta ggacacatca accccgatac cgactcgatc gcctctgcca
     2701 tgggatatgc ctggttactg cgcgaacgtg acgggcttaa caccatagcc gcccgcgccg
     2761 gagccttaaa cgcacaaact tcatgggtat tgaaaacact cgggctggaa tctccatttt
     2821 tattgaccga tgcttcaccc cgctttgact ccgttatgca aaggctcgat agtctacgtc
     2881 ccaacgccca actcggcagc gcatggactc tcgccagccg cacgggcggg cttgccccgg
     2941 ttgtcaatga agatggcacc ccgtatggca tcatcagcgg catgagcttg ttcaaatatt
     3001 tcagcaatat cctcggtccg cgccccggcg atacaactgt gcgcgaaatg atgtcagctg
     3061 aatgtaaaga tgctgcagat accagtgtgc caaaatatca agccaacgcc cacatccgcg
     3121 attcgctcaa ccgcattctg cgcgatgaga tcgacgatta ttgggtcgta gatgaaagcg
     3181 gaagatatct cggcattgcc cgtcaacgca atttgctgaa tccgccgcgc ctcaagatca
     3241 tcctcgtgga tcacaatgaa ccgcgccagt ctattgctgc ccttgaagaa gccgaacttc
     3301 tggaaattct cgatcaccat cgcctcggca acccctatac ccatcagccc attcgcttca
     3361 cagtggatgt ggtcgggtcc acttcgactc tcgtcaccga acagacggct gaagcgggct
     3421 tgtctgcccc gcctgccatt gcgggtgcgc ttctttctgg acttttgtcg gatacgctca
     3481 tcctaacctc tcccaccacg acagaccgtg atcgcgtcgc cgcagaacgg ttggcacgct
     3541 gggcgtttgt tggaggcagc cccctacaag gcgagaccat cgaaacctat ggaaaagccg
     3601 ttcttagtgc aggtgctggg ctttccaacc gcaagccaga agaagtcgtc agcacagata
     3661 ttaagccatt tgaaggcggc ggattcaaat ttgcagtggc tcaagccgaa gtgaccgaca
     3721 gcatgcaact ttcagaacac cttgcaccac tcaccaaagc cctcgatgac ctacgcgaca
     3781 aacgcggact cgactttgcg ctgttactgg tcactgatgt ggtgcgtgga accagtcgct
     3841 tgatcgttac atcaagtgcg cctcccattc tcaatgacct tccctacccg cccttacccg
     3901 acggcactcg cgatgcaccc ggagtggttt cgagaaagaa acagttgttg ccagccgtat
     3961 taggtttatt agaacgataa gtaaatgtcc atatagggcg ggttttgaac ccgccttatt
     4021 ttgtataatt ttgaaaaacc atgcccttcc catacctcga cgaaaccctt ttactgaatg
     4081 ccgaaacccg taaagacttg ccgggctcgt tcatcaccct gcctagcgga gtaacacact
     4141 acgaagaagg cggggatcca aagggtcagc cggtggtctt ggtgcatgga ttttctgtcc
     4201 cttactttat tttcgaacca acttttgtat ttctttgtca gcaaggcttt cgtgtcttgc
     4261 gctatgactt gcttgggcgt ggattttcag atcgtccaac cacaagatac aacgcatccc
     4321 tgtttgtcca acaaatcaaa gaattgttgg atgcactgaa ctacaaaaat atctgtcttg
     4381 cagggctatc tatgggtgga gcggtcacct cttcgtttat tgatgcgtac cctgattatg
     4441 tttcaaaata cgttctcatt gacccatctg gcgcaagggc agtttcgctc tcgccttttc
     4501 tcaaagcggt gagaatgccc gggtttggcg aactggctat cggtctgttc ggaagcgaaa
     4561 gtatgcttaa aggcattgca tcagacttgt tcagtccaga acttgtgcgt gaattccaag
     4621 aaaaatataa agtccaaatg caatatcaag ggttcaagcg tgccattctt tctacgatgc
     4681 gcaatggcat gttggactct tttattgaga cctacaaacg agttggcaaa ctcaaaaaac
     4741 cgactcttct tttttgggga aaacaagata caacaactcc gtttgagcat agtgccgata
     4801 tatgcgcagt gatgccgcat gcagaattct atgcctttga cagttgtggg cacattcctc
     4861 actacgagcg cgcaagcgag gtaaacagaa ttctgttaga atttttgagg aagtaattcc
     4921 ctcaaaacaa atacatagtg attgggggat ggattaaagg agcaaccgtg aacaaagaat
     4981 atattcaaac gttgtaccgc tataaccagt ggtcgaatgc gcggattctc gacaccgccg
     5041 ccaaactcag ccctgagcaa tttttagcaa cttcagaaca tccacaccgc tatttacgcg
     5101 gtgtccttac acacaccctc tttgccgaat ggatttggca caaacgctgg acaggcgaat
     5161 cacccactac gcgcttgaag ccggaagact tcccaacctt cgaagcattg cgtacacgtt
     5221 gggcagagga agaaagccaa ttaatgcaat tcgttgcgca agtaacggac gaaaaactga
     5281 acgcgccttt tacctattta gccaccgatg gcacaaaata tgaaaatatt ctgtgggagt
     5341 cgatggcaca tgtggtcaat catggcatgc aacatcgcag tgaagccgcc gccattctca
     5401 ccgaacttgg ggtttctccc ggcgatttag atttcatcct attcacgaga aagaaactat
     5461 gaccttcaat cgcgccaaga attttctttt tcggaatcgt cataacctag ctgtgttcgt
     5521 cttgcttaac ctggcatgcg tggcttgtat tgtttttgta gcagcgcgtg tggtgtatac
     5581 cgattcgtcg cgtcacacgg gtttgatttg gaatctcttc ctggcatgga ttccgttcat
     5641 ccttgcgtat tttgctcatg ctctttcgtg gaaaaaagtt tgggtatatc tggcaattcc
     5701 atttgtggct tttctgtggc tattgttctt ccccaacgct ccatacatgt tgaccgacct
     5761 gcaagacctc gctcgcggca cgggttatga agctccgctt tggtacgatg tcatcatcgt
     5821 cgtctggtgc tcgtggacag gaactttgct gggcgtcatc tcgctctatt tgatgcaaga
     5881 cattatcgcc cgcaccttcc accgctggct gggatgggtc ttcgtctttg caatttcagg
     5941 gctgagcagt tttggcattt acatcggtcg cttcgtgcgc ctgaattcat gggacatttt
     6001 gcaaaacccc gccgaaaccg ccatggaaat tctcggcatc gtcattgacc caagcaagcg
     6061 tctcgccgcc tttacgttgg catatacctt tttcttctta ttcatttacc tgttgttgta
     6121 ttcatttagt cacatgttac aagaacaggt gatccgtgct caaagctctc cggagcaa
//
LOCUS       DAOQFT010000066         6093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6442_length_6093_cov_7.412388, whole genome shotgun sequence.
ACCESSION   DAOQFT010000066 DAOQFT010000000
VERSION     DAOQFT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_6093_cov_7.412388"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..304
                     /locus_tag="PLR93_02750"
     CDS             26..304
                     /locus_tag="PLR93_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25908.1"
                     /translation="MSKLSPRMLLLAALVLILVAGAFTTYRAVSANSERNSMGMYVLS
                     GGLVNQLKPQQPEGGTTDQNQVAPFPGSSGEGGHGGCESENNINPSDL"
     gene            396..2294
                     /gene="dnaG"
                     /locus_tag="PLR93_02755"
     CDS             396..2294
                     /gene="dnaG"
                     /locus_tag="PLR93_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNH25909.1"
                     /translation="MSTIDEIKSKIDIVDLVSEAGVKLRKAGRNMTGFCPFHDNKHTP
                     AFVIWPESGTWRCFGACNEGGDIFKFVMKREGIDFKEALNKLALRAGVEVQQIQRETP
                     QQREAYDHLRTLLEDAVVYYRSHLFANPEILNYLRQKRGLTDATIETFGLGYAPLGYD
                     NLLKHFSGKYMPQDLVDAGMLSERDSGGLFDRFRHRIMIPIRDENGKMAGFGARIVDP
                     NDNPKFLNSPETAIFTKGRLLYGFDRARKPIRAADQAVIVEGYLDVIALHQAGYENVV
                     SPMGTALTEDQLRLLKRSTRRIVLALDPDVAGQKAVLRGLEAARSAMDKEGELTFDAH
                     GLLRNEARLQADLRVATMPDDLDPDEIVARNKEEWATLIANAKPIITHVMEALSAGQN
                     LNDPKIKNQIAEQVLPLIDDMGTPMERDTYRQALARMLRVDERTLMAAQPKGAVMRRK
                     PRVTEPSRRTESVVVAAVNPRKNIEFYCLSVLIRKPDLVYRLDRKLEEFGLSPLATED
                     FEYTDHQLLFNVLRQAMGQDEKDHAQYVFSQIPEDLAPLVNELLAQTEKLESSDDKLL
                     EDLLARFLDLRRFHAMSSVTQLKFMQDDEQQQGGENIKVYIEQTMRFTRLLNGLDQAK
                     LKLSKRQA"
     gene            2465..2659
                     /locus_tag="PLR93_02760"
                     /pseudo
     CDS             2465..2659
                     /locus_tag="PLR93_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            3128..3937
                     /locus_tag="PLR93_02765"
                     /pseudo
     CDS             3128..3937
                     /locus_tag="PLR93_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            4139..4495
                     /gene="ndhC"
                     /locus_tag="PLR93_02770"
     CDS             4139..4495
                     /gene="ndhC"
                     /locus_tag="PLR93_02770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNH25910.1"
                     /translation="MLLEYIAIGVMILVATLIAFIAIGLGELFGPKKKSAGKDTPYES
                     GMNPYGEGTRRMPVRFYLIAVLFILFDIEVVFFLPWAIAFRKLGLFGLIEMVVFITVL
                     LIGYVYAWKKGALEWE"
     gene            4486..4980
                     /locus_tag="PLR93_02775"
     CDS             4486..4980
                     /locus_tag="PLR93_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNH25911.1"
                     /translation="MGVEQKLGNMGVVTTTLENVVNWSRTNAMWPMLFGLACCAIEMM
                     AAQAAHYDMSRFGMELMRASPRQSDLMIVAGRVSKKMGPVLRRLYDQMPEPKWVIAMG
                     DCASCGGVFNNYAIYQGVDEVVPVDVYVAGCPPRPEALIHGVMTLYEKVNAMKLAEKP
                     AESK"
     gene            5031..5543
                     /locus_tag="PLR93_02780"
     CDS             5031..5543
                     /locus_tag="PLR93_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNH25912.1"
                     /translation="MEKNLEPIVKDLQTKFGATYEEFRGEVQCMVKAENIVDVLKTLR
                     DEHSFELLSVLNAIDYYPQTSPRFHVVYQLNSLSKNLRVQVRVPVNDDQPKVPTVTGV
                     FKTGMWREREVFDMFGIEFEGHPDPRRILMPEGTEGHPLRRDFPLGYEEPQFTFNLEE
                     IDLRKPYAKE"
     gene            5547..>6093
                     /locus_tag="PLR93_02785"
     CDS             5547..>6093
                     /locus_tag="PLR93_02785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNH25913.1"
                     /translation="MTQLQEVSVEQFKHLVSERALTGETMLLNMGPQHPSTHGVLRLL
                     LELDGETVVNCIPDIGYLHTGIEKNMEAKTYQKAEVMTDRLDYMNPMGNNLSYVMAVE
                     KLVDLDVPPRAQALRVILTELQRISSHLVLIGTWGLDLAAMSMFLYCFREREQILDIF
                     ELVSGQRMMTTFFRPGGLWRDV"
BASE COUNT         1530 a         1437 c         1564 g         1562 t
ORIGIN      
        1 tatttacacg taaggagaaa atcgtatgtc taagttatcc cctagaatgc ttttactcgc
       61 agccttggtt ctgattcttg tcgctggagc gttcaccacg tatagagcgg tttcagcgaa
      121 ctctgaacgc aacagcatgg gcatgtatgt gctcagcgga gggttggtca accaattgaa
      181 accgcaacaa cccgaaggtg gaacgacaga ccagaatcag gttgcccctt tccctggcag
      241 cagcggagag ggtggtcatg gtgggtgtga gtctgagaac aatatcaacc ccagcgactt
      301 gtaaaatgga ttttgtaaaa ataaggcagc gaaatggctg ccttatttta tttatcgttt
      361 gcacatccta ccaatcgcgc ctataattca cgtgcatgtc caccattgat gagatcaagt
      421 ccaagataga tatcgttgac ctcgtatctg aagcgggggt gaagttacga aaagccggtc
      481 gcaatatgac cggtttttgt ccgtttcatg ataacaagca cacgcctgcg tttgtgatct
      541 ggcccgaatc aggtacatgg cgctgttttg gggcatgcaa tgaaggcggg gatatcttca
      601 agttcgtgat gaagcgtgaa ggcatcgact ttaaagaagc cttgaataaa cttgcgcttc
      661 gagccggtgt ggaagttcaa cagatccaac gtgagacgcc gcaacaacgc gaagcgtacg
      721 atcatttgcg gactctgttg gaagatgccg ttgtttatta tcgcagtcat ttatttgcaa
      781 accctgagat attgaattat ctgcgccaaa aacgtgggtt gacggacgct actattgaga
      841 cctttggctt ggggtatgcc cctctaggct atgataatct gctcaaacat ttttcgggca
      901 agtatatgcc gcaggatctt gtagatgcag gcatgctctc tgaaagagat agcggcggat
      961 tgtttgatcg ttttcgtcat cgcattatga ttcccatccg cgatgagaac ggcaagatgg
     1021 caggctttgg cgcacgcatc gtagacccga acgataaccc caaatttttg aattcacctg
     1081 aaacagccat ctttaccaaa gggcgtttgt tgtatggctt tgaccgcgct cgtaagccca
     1141 tccgcgcggc agaccaagct gtgatcgtcg aagggtatct cgatgtgatc gccttgcatc
     1201 aggcgggtta tgagaatgtc gtctcgccga tgggcacagc actgaccgaa gaccagttgc
     1261 gcttgctcaa gcgttctacc cgcagaattg tccttgcgct tgacccagat gtggcaggtc
     1321 agaaagccgt gttgcgcggg cttgaagcgg ctcgctctgc catggataaa gaaggggagc
     1381 tgacctttga tgcacatggc ttgctccgca acgaagcccg cctccaagcg gacttgcgtg
     1441 tagcgaccat gcccgatgat ctcgaccccg atgagattgt ggcacgtaac aaagaagaat
     1501 gggctacatt gatcgcaaat gccaagccta tcatcacgca tgtgatggaa gccctttctg
     1561 ctgggcagaa tttgaacgac cccaagatta agaatcagat cgccgaacaa gtgttaccgc
     1621 tgatcgatga tatgggtacg cccatggaac gcgatacgta tcggcaggcg ttggcgagaa
     1681 tgttacgggt tgacgaacgc accttgatgg ctgcccagcc caaaggcgcg gtgatgaggc
     1741 gcaagccacg tgtcacggaa cccagccgca gaacagagtc tgttgtggtt gcggcagtga
     1801 atccaagaaa aaatattgag ttctattgtt tgagcgtttt gattcgcaag ccggatctgg
     1861 tctatcgttt agaccgtaag ttggaagagt ttggcttgag cccattggca acagaagatt
     1921 tcgagtatac tgaccatcag ttacttttca atgtattgag gcaggcgatg ggacaggacg
     1981 aaaaagatca tgctcagtat gtgttctcgc aaattcctga agatcttgct ccccttgtca
     2041 atgaattgct tgcacaaaca gaaaaacttg aatcatcaga cgataaattg ttggaagatc
     2101 tcttagcacg ctttttagac ctgcgacgtt tccatgccat gagcagcgtc acgcagttga
     2161 agttcatgca agatgatgaa caacaacaag gcggagagaa catcaaggtc tatattgaac
     2221 aaacaatgcg ctttacccgt ttactaaacg gtttggatca ggcgaagtta aaattgtcga
     2281 aacgacaggc gtaattcaac ccgctttgta ggagacaggt tccatgaaac cgaaacgaac
     2341 gtctgccagc aaaacaacga agaaaaaggg catggaaaaa gaaaagaaaa aggctgtgaa
     2401 gcaaacatcg cccgaaaagc cccgcaaggg tcttcgttcg ggtggctcca taggggatga
     2461 atccatgcca gagtttgtca cggatgattt tgccgagccc gatgacacgc tctttgtgcc
     2521 acaagaagaa gaacttgatg aatctataaa tatacatgcg catttaactg gcgagccggg
     2581 cgaagaccca gtgcgtttgt atttgcgcga gatcggtcag gtcaaattat tggcggcaga
     2641 tagcgagttt cgccttgcca ctctgatcga aggcgaccgc cttatttcca ctttgaataa
     2701 actcaaacaa cgcaaagggg tttcgcaggc ggcgtccact tatcattccg tgctttctga
     2761 agcgcttact tcttgggatc gattggtgga ggataccgag cgccttgaac atgacttgcc
     2821 caacttggcg ctcttgctgg cagaggctca atcgcttcat gccggatggg aatttgactc
     2881 tccatcctat ctgcgtgcct acctcgacaa cggtcagtgg gggagtgacc acctatggga
     2941 taacctggcc cgtcatgctt atagtgtttt cctaagcctg tatctactac catatcccta
     3001 tgcggaatgg ttgttgtcgc atgtgcgtgt gcatcaaggc tttcccgctc agcggacctt
     3061 gttccgtaat ttgccatccg atgaagagtt gatttctgaa atggaatccg tccatttgcg
     3121 cgccatcgat gctcaacagg cattggtccg cgctaatttg cgtttggtag tgagcgttgc
     3181 caaacgttat ctggggcgtg gcattccgtt attagacttg atccaagaag gcaatatggg
     3241 gcttctgcgc gccatcggca agtttgaccc acggcgcgga ttcaaattca gcacctatgc
     3301 cacttggtgg attcgtcagt ccattaaccg ttccatcgcc gaacaagcgc gtaccattcg
     3361 cattcccgta catctctttg agtctatttc gcgcatcttg cgcgcccaac gtcacctcac
     3421 gcaggttttg ggtcgtgaac ccacgaacga agatatcgct ttggaagttg gctatctctc
     3481 tgctgtggat gtgcaatcca ttttgcgggc gcatgccgag agtaagcctg tccacccgga
     3541 attgcaacgc aagttagatg tcgcttcgca aaaggtcact cgtgctttgc gctctgccga
     3601 agaaaccatc tcgcttgaag gtccggtggg ggatgacgaa tccagcgctt tgggcgattt
     3661 cattgaagat gaagacgcgc tttcgcccat ggacgctgcc gcccgcgaaa tgctgcgtga
     3721 gcaaattacg cacgccttgg aatctctttc agaccgtgag cgcgacgtct tggaattgcg
     3781 ttttggactt aaagatggaa aagaacatac cttggaagag gtcagtcgct tctttgatgt
     3841 gacccgtgaa cgggtgcgcc aaattgaagc caaagcactc cgcaaacttc ggcacccaag
     3901 caagagccga gaattaagag attatttagg tgattaataa accaaagtga cagtttccat
     3961 aacgagactg tcactttttt gttttcttgg agtgttttct atgaattcga ttacattttg
     4021 cccaacatga caaaaatcat catttttgtg taatgtagca caagcgctgt gcttaaaaaa
     4081 acctttgtga tatactttcg cgaactgtgc aagatttcac acatccatag aggtgtttat
     4141 gctgctggaa tatattgcca ttggggtcat gatcttagtg gccacgttga tcgcttttat
     4201 tgcgatcggt ctaggtgagc tttttggtcc aaaaaagaaa tctgcgggga aagatacgcc
     4261 ctatgaatcg ggcatgaatc catatggtga aggaacgcgt cgtatgccgg tgcgtttcta
     4321 tttaatcgcc gtattattca ttctgtttga tattgaagtt gtattctttt taccttgggc
     4381 gatcgcattc cgcaagctcg gtctcttcgg cttgatcgag atggtggttt tcattacggt
     4441 ccttcttatc ggttacgtct atgcttggaa aaaaggagct ttggaatggg agtagaacaa
     4501 aaactcggaa atatgggcgt ggtaaccacc acgcttgaaa atgttgtgaa ctggtcacgc
     4561 accaacgcca tgtggcccat gttgttcggt ttggcttgct gtgccattga gatgatggct
     4621 gctcaagccg cccactatga tatgagccgc ttcggtatgg aactcatgcg tgccagccct
     4681 cgtcagtctg atttgatgat cgtggctggg cgtgtctcca aaaagatggg acccgtcctt
     4741 cgccgtttgt acgaccagat gcccgaaccc aagtgggtga tcgccatggg cgattgcgct
     4801 tcttgtggcg gcgttttcaa taactatgcc atctatcaag gtgtggatga agttgtgcct
     4861 gtggacgtgt atgtggctgg ctgccctccg cgccccgaag cattgatcca cggcgtgatg
     4921 accttgtatg aaaaagtcaa cgcgatgaag ctggcagaaa aacccgcaga atcgaaataa
     4981 aaaaaacaga cctgacagat ttcaagacct gtcaggtctt aggtgtattt atggaaaaaa
     5041 acctcgaacc tattgtaaaa gatctgcaga cgaaattcgg cgcaacgtac gaagagttcc
     5101 gtggtgaagt gcaatgtatg gtgaaagccg agaatatcgt tgacgtgtta aagaccttgc
     5161 gcgacgaaca ttccttcgaa ttactctcag tgcttaatgc cattgattac tacccacaaa
     5221 cctcacctcg ttttcatgtt gtgtaccagc tcaattcact ctcaaaaaat ttacgggtgc
     5281 aggtgcgagt ccctgtcaac gatgaccagc ccaaagtccc gaccgtgacc ggcgtgttca
     5341 agaccggcat gtggcgcgaa cgtgaagtct tcgatatgtt cggcatcgag ttcgaaggtc
     5401 atccagaccc gcgccgcatt ctgatgcccg aaggcaccga gggacacccg ctgcgcagag
     5461 acttcccgct tggctacgaa gaaccgcaat tcactttcaa cttggaagag atcgaccttc
     5521 gcaagccata tgcaaaggaa taatttatga cacaactaca agaagtgagt gtagagcaat
     5581 ttaaacatct ggtttcagag cgcgcgctga ccggcgagac catgctcttg aacatgggtc
     5641 cgcaacatcc atccacgcat ggtgtgttgc gtttgttgtt ggaactcgac ggcgaaacag
     5701 tggtgaactg tattcctgat atcggctatt tgcataccgg tattgaaaag aatatggaag
     5761 ccaagaccta ccaaaaagcg gaagtgatga ccgaccgcct tgattatatg aaccccatgg
     5821 gaaataacct ctcctatgtg atggctgtcg aaaagttggt agacctggat gtgccgccgc
     5881 gtgctcaagc tttgcgcgtt atccttacag agcttcaacg tatttcctct cacctcgtgt
     5941 tgatcggtac atggggcttg gacttggcag ccatgtccat gttcttatat tgcttccgcg
     6001 aacgtgaaca gatcctcgat atttttgaac tcgtctctgg tcaacgcatg atgaccacat
     6061 tcttccgccc cggcggtttg tggcgcgatg tac
//
LOCUS       DAOQFT010000067         6086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6455_length_6086_cov_6.069474, whole genome shotgun sequence.
ACCESSION   DAOQFT010000067 DAOQFT010000000
VERSION     DAOQFT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6455_length_6086_cov_6.069474"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..524)
                     /gene="smpB"
                     /locus_tag="PLR93_02790"
     CDS             complement(63..524)
                     /gene="smpB"
                     /locus_tag="PLR93_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNH25914.1"
                     /translation="MTDNIKIIANNRKAGFEYTLIETFEAGISLQGSEIKSIRAGQIS
                     IQEAYVDVENGQEAWLLEAHIAPYEQAGKYFNHEPKRKRRLLMHKKEIRELWNNVRIK
                     GMTIVPTKVYLKDGRAKVEIALAKGKKAYDKRATIAKRDEARDKERAMRVR"
     gene            complement(597..1850)
                     /locus_tag="PLR93_02795"
     CDS             complement(597..1850)
                     /locus_tag="PLR93_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH25915.1"
                     /translation="MRFNLFKQSDIPEKYRSNFLHLYFDIAWFGVLSGTAVNFLNVYA
                     TRLGASASQVGLLTAASAVVNLFLAIPAGHWISKRHTGMAVFWSSVLFRIGYFMWIPL
                     PWFLSNQGQIWALIILTFLMAIPLTPLGVGFNALFAEAVPDRYRARVAGTRNVTFAIA
                     YMLTSLVAGYILKNTEFPGGYQVIFVIGAFGAAMSSYHIYHVKPMQDDTPAAPSAPVP
                     ASAPKPDSPRGMTSALRLDIWKSPFKNVLLALFIFHFAHYLSNPLYPIFNVRVLNLND
                     SHLGNGTALYYLSVLLASTQLGRIVHRFGNKRITGWGVAGMASYPLMLAFAHTVWQFY
                     VLSFLGGFLFALVNGSYANYMLERIPADDRPSHLAWYTLMFNFAILTSSLIGPAVAEV
                     IDLVPAMIAFGIARILAGLFLLKWG"
     gene            complement(1917..2168)
                     /locus_tag="PLR93_02800"
     CDS             complement(1917..2168)
                     /locus_tag="PLR93_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNH25916.1"
                     /translation="MSQPNITVYGADWCGDCRRSKRFLTEKNIPFTWVDVDQDQAGEA
                     LVLEKNNGKRIIPTILFEDGTFLAEPSDEALADKLGLPV"
     gene            2300..3052
                     /locus_tag="PLR93_02805"
     CDS             2300..3052
                     /locus_tag="PLR93_02805"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNH25917.1"
                     /translation="MRFKDKVAIITGGTSGIGLAVAELLVREGAQVALFARDTKRGQE
                     TIQTLGQNAIFIAGDVGVAADCQHCVEKTIEAFGRLDILFNNAGIIHVNRNVVTTLED
                     EWDATLNSNLKGTYLMSKYAIPYMEKQGGAIVNNASIFGLVGGGGVAAYCAAKGGVIN
                     LTRAMAIDHAAQNIRVNCVCPGSVDTPLLEKEMDDLGGADLQRPKFASRHPMNRIATP
                     EEVANAVAYLASDAASFVTGTALPVDGGRAAW"
     gene            complement(3054..4271)
                     /locus_tag="PLR93_02810"
     CDS             complement(3054..4271)
                     /locus_tag="PLR93_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH25918.1"
                     /translation="MTEPKITLYGAYWCPDCRRSKKFLGEQFVPFKWIDIEQDKEAEA
                     LVLQKNNGKRIIPTIVFEDGSFLVEPTNAELAKKLGLKTEAKKTYYDLIIIGGGPAGL
                     TAAIYASREGADVLLIERSGLGGQAGITVGLDNFPGFPEGISGQEFSDRVTQQAKRFG
                     VEILQAVDVERLEEENGYHEVYTSDGKHYHSRAVLLATGASYRRLEVPGEDDYIGAGI
                     HFCATCDGPFYKGAKEIVVIGGGNAAVEEGLHLTNFAEKVTLLARGDKLTASQIAIDK
                     VTEPGSKVDVRYNVSVEGFDGQDSKLKSIKTRNKVSGQVEELHPAAAFVFIGQQPNTE
                     FVKSYVQTDPSGFILTGHDLGHDQDALPFETSVRGIFAAGDARHGSVKQVASAVGEGA
                     AVSISIREFLRKG"
     gene            4425..4928
                     /locus_tag="PLR93_02815"
     CDS             4425..4928
                     /locus_tag="PLR93_02815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25919.1"
                     /translation="MNAHQNVAGRDNIFIAIAPDGDQIKDITARAGVFSEEEVTCVAE
                     IWDDYIENGVESCGYNFIVYREGTQVLGFASYGYRDLTDGVYDLFWIAVDANTRRGGV
                     GRKLLSATEEEVRNLGGRMVIAETSGKALYESTRKFYIGTGYQAEATIKDFYTKGDDL
                     VIYVKRL"
     gene            5001..>6086
                     /locus_tag="PLR93_02820"
     CDS             5001..>6086
                     /locus_tag="PLR93_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH25920.1"
                     /translation="MATLKKFFLLDRSITFLNHGSFGATPKPVFDEYQRWQRELEHQP
                     VEFLGRRFAGLMRTAREALADYLGTSAGNLIFAQNVTIALNVVARSLELGAGDEVLST
                     HHEYGAMDRTWRFLSKERGFTYINQVVDISSPDAFVDSFWRGVTSNTKVIFLSHITSP
                     TATIFPIEKLIQRARAQGILTIIDGAHAPGQIPLHLDALGADFYGGNLHKWLCAPKGA
                     GFLYARPEVQHLLKPLVVSWGYEAEAPGDSQFIDYNEWWGTRDVSAFLAVPKAIQFQQ
                     DQNWAEVRAACHRLAVETWQRIHDLTGETPLHSDPEPWFAQMAVATLPADSDLVSLKS
                     RLYDEYRIEIPLIAWNEKKLIRLSVQGY"
BASE COUNT         1520 a         1461 c         1558 g         1547 t
ORIGIN      
        1 gcatagcttc ttctttcttc tttcacctct cctccctata taaagaggaa agatgaaaga
       61 ggtcatctta cccgcatggc acgttcttta tcacgcgctt catctcgttt agcaatggtg
      121 gcgcgtttgt cgtaggcttt cttgcctttt gccagcgcga tctcgacctt ggcacgcccg
      181 tctttgagat agaccttggt tgggacgatg gtcatgcctt tgatgcggac gttgttccaa
      241 agctcgcgga tctctttttt gtgcataagt aaacggcgtt tgcgcttcgg ttcgtggttg
      301 aagtatttac ccgcttgttc ataaggcgca atgtgtgctt cgagcagcca ggcttcctgt
      361 ccattttcca catccacata ggcttcttga atgctgatct gcccggcacg aatggatttg
      421 atctcagatc cttgcaggga aatgcccgct tcgaaggttt cgatgagagt gtattcaaaa
      481 ccggctttac ggttgttggc gatgattttg atgttatctg tcacaagatg attttagcat
      541 ggaaaattgt tggggaataa gccacagaac ccgcagagat tttgagggct agttgattag
      601 ccccatttga gcaggaacaa cccagcgagg atgcgggcaa tgccaaaggc gatcatggca
      661 gggacaaggt caatgacctc tgccacagcc ggaccgatca acgagctggt gaggatggca
      721 aagttgaaca tgagggtata ccacgccaaa tgcgaagggc ggtcatcggc ggggatgcgc
      781 tcaagcatgt agttggcata tgaaccattg accaaggcaa acagaaagcc gccaaggaat
      841 gaaaggacgt aaaactgcca aacggtgtgc gcgaatgcca acatcagtgg gtacgaagcc
      901 atacccgcca cgccccagcc tgtgatgcgt ttgttcccaa agcgatggac gatgcgccct
      961 aattgagtgg atgccagcaa tacagataga tagtagagcg cggtcccatt gccgaggtgg
     1021 ctgtcattga ggttgagcac acgcacgttg aaaatcggat acagcggatt tgacaggtag
     1081 tgtgcaaagt gaaagatgaa caatgccagc aagacattct tgaatggcga tttccaaatg
     1141 tccaggcgca gggcggaggt catgccgcgg ggagagtctg gtttgggggc agaggcaggc
     1201 acgggcgcgg aaggagccgc aggcgtatcg tcttgcatcg gtttgacgtg gtagatgtga
     1261 taactgctca tcgccgcgcc aaaggctccg atcacaaaga tgacttgata cccacctggg
     1321 aattcggtgt tcttgagaat gtaaccagca acgagggagg tgagcatgta ggcgatggcg
     1381 aaggtcacat tgcgagtgcc tgctacgcgg gcgcgataac ggtctggcac ggcttcggca
     1441 aagagtgcgt taaatcccac gcctaatggc gtaagtggaa ttgccatgag aaaggtcaaa
     1501 atgatgagcg cccaaatttg cccttggttg ctaagaaacc aaggcagggg aatccacatg
     1561 aaatagccaa tgcgaaagag caccgaagac caaaagaccg ccataccggt gtgacgtttg
     1621 ctgatccaat gcccagcagg aatagcaagg aaaagattga cgacggcaga ggctgctgta
     1681 agcaagccga cttgcgaagc gctcgccccc aagcgcgtgg catacacatt gagaaagtta
     1741 acggcggtgc cgcttagcac gccgaaccag gcaatatcga aatagagatg aagaaagttt
     1801 gatctgtatt tttcaggaat atctgattgt ttaaataaat tgaaacgcat acatgaaatt
     1861 atactcacgt atgcgtttcg agattttgca gcttatttga ttttcaggtt gttttgttag
     1921 acgggtagac caagtttatc tgccagcgct tcgtcagatg gttcagcaag aaaggtgcca
     1981 tcttcaaaaa ggatagttgg aatgatgcgt ttgccgttgt tcttttccaa aacaagcgct
     2041 tctcctgctt ggtcttggtc gacatcgacc catgtgaagg ggatattttt ttcggtcaaa
     2101 aagcgtttag agcgccgaca gtcgccgcac cagtctgcgc catagacggt gatattgggt
     2161 tgtgacatga attcctccgg tgatgaaaac tgaaatgagt gtacttccgc taccgtcgcg
     2221 ctgtcagtgc tattaattac attttgggag gaataaaatt acaatgtctt cattcaagca
     2281 tacttctgga gaaaaaaaca tgagattcaa agacaaagtt gcgatcatta caggtggcac
     2341 atcgggtatt ggtttggcag tggcggagct tctagtgaga gaaggcgctc aggtagcctt
     2401 atttgcacgc gacaccaaac gtgggcaaga aaccatacaa acgctggggc agaacgcaat
     2461 ttttattgca ggcgatgttg gcgttgctgc ggactgccaa cattgtgtag agaaaactat
     2521 cgaagccttt ggtcgtttgg atatcctgtt caacaatgca ggcattattc atgtcaaccg
     2581 caatgtggtg accactttgg aagatgaatg ggatgcgacc ttgaattcca atttgaaagg
     2641 cacatatctc atgtcgaagt atgccattcc atatatggag aaacaaggcg gcgcgattgt
     2701 caacaatgct tcgatctttg gcttggtggg tggcggcgga gtcgctgctt attgtgccgc
     2761 gaaaggtggt gtgattaacc taacacgtgc catggcaatt gaccatgccg ctcagaacat
     2821 ccgcgtcaat tgcgtttgcc cgggcagtgt ggatacgcct ttgcttgaaa aagaaatgga
     2881 tgatttgggt ggcgcggacc tgcaacgccc caagttcgct tcacgccacc cgatgaatcg
     2941 gatcgctacg cccgaagaag ttgccaacgc tgtggcgtat ctcgcctcag acgctgcatc
     3001 ttttgtaaca ggcacagccc tgcctgtgga tggcggcagg gctgcctggt aaattaacct
     3061 ttgcgcaaaa actcgcggat cgagatcgaa accgccgcgc cttcgcccac tgccgacgcc
     3121 acttgtttaa cactaccatg ccgcgcatcg cctgccgcaa agatgccacg aacactggtt
     3181 tcaaatggca aagcatcttg atcgtgcccc aagtcgtgac ccgtcagaat aaatccgctt
     3241 gggtcggtct gcacatagct tttcacaaac tcagtatttg gttgttgacc gataaacaca
     3301 aacgctgccg ctggatgcaa ctcttccacc tgaccggaca ccttattacg ggtcttaatg
     3361 gactttagtt ttgaatcctg tccatcaaac ccttctacgc tgacgttgta tcgcacatcc
     3421 acttttgagc cgggttcggt cactttatca atcgcgatct ggctggcagt cagtttatct
     3481 ccgcgtgcca agagggtcac tttctcggca aagttggtca gatgcaaacc ttcttccacg
     3541 gcggcatttc caccaccgat aaccacgatt tcttttgcac ctttataaaa cggaccatca
     3601 caagtggcac agaaatgaat acccgcccca atgtaatcat cctcaccggg cacttccagt
     3661 ctgcgatatg atgcgcccgt agctaacaag acagcacgtg aatgatagtg cttgccgtct
     3721 gatgtatata cttcgtgata gccattttct tcttccagcc gttccacatc tacggcttgc
     3781 agaatttcta cgccgaagcg tttagcttgt tgcgtgacac gatctgagaa ttcttgtccg
     3841 ctaatgcctt caggaaagcc gggaaagtta tctaacccaa ccgtaatccc cgcttgcccg
     3901 cccaaaccag agcgttcgat cagcaacaca tctgcacctt cacgtgaggc gtagatcgcg
     3961 gcggtcaacc cggcaggtcc gccaccaatg atgatcaggt cataataggt tttctttgct
     4021 tcggttttca atcccagctt tttggcaagc tcggcgttgg tcggctcgac tagaaatgac
     4081 ccatcttcaa acacaatggt tgggatgatg cgcttcccgt tgttcttttg aagcacgagg
     4141 gcttcggctt ctttgtcctg ctcaatatcg atccacttaa aagggacaaa ttgttcgccc
     4201 aaaaatttct tcgagcggcg gcagtctggg caccaatagg caccatacag ggtgatcttg
     4261 ggttcggtca tttcttcctc cggtaaattg atttgctgta ctggtgtgat gtacttgcat
     4321 ttctttacta ggacaatctt cctactctag atgataaaat tctaaaaaaa cggcttcaaa
     4381 caaagcctac acctaaaatt caaaacagga aggcatttca acccatgaat gcacatcaaa
     4441 acgttgccgg gcgagacaat attttcatcg ctattgcgcc cgatggagac caaatcaaag
     4501 acatcaccgc ccgagcaggt gtttttagtg aagaagaagt cacctgtgta gcagagattt
     4561 gggacgatta tattgaaaac ggcgtagaaa gctgtggcta taacttcatc gtctatcgtg
     4621 aagggactca ggtgctgggc tttgcttctt atggctaccg cgacctgacg gatggtgtat
     4681 acgatttgtt ctggatcgca gtggatgcca acacccgtcg tggtggagtg gggcgcaagt
     4741 tattaagtgc tacagaagaa gaagtgcgca acctgggcgg gcgcatggtg attgctgaaa
     4801 cctcaggcaa agccctctat gagtcgactc gtaagtttta tattggaacg ggctaccaag
     4861 ccgaagcgac cattaaggac ttttatacca aaggtgacga cctcgttatt tatgtgaaac
     4921 ggctttagga tttatagcac tctcaaacgc cgtccgcata aactatgtgg acggcgtttt
     4981 atttcccgta taatttctat atggctactc tcaagaaatt ctttttgctc gatcgctcta
     5041 taacattctt aaatcacggc tcatttggtg ccaccccaaa accggtattt gatgaatatc
     5101 aacgttggca aagagagttg gagcatcagc cggttgaatt tcttggcagg cgttttgccg
     5161 ggctcatgcg cactgcacgt gaagcactag cggattatct aggcacaagc gctggtaact
     5221 tgatcttcgc acaaaatgtg accattgcct tgaacgtcgt tgcacggtca ttggagctgg
     5281 gtgcgggcga tgaagttctc tcaacccatc atgaatacgg ggccatggat cgcacctggc
     5341 gtttcctttc aaaagaacgc ggctttacgt atatcaatca agtagtcgat atctcttcgc
     5401 ctgatgcctt tgtcgattcg ttttggcgag gcgtaacttc taacactaag gtcatttttc
     5461 taagccatat tacttcgcca accgcaacca tcttcccgat agagaaatta attcaacgcg
     5521 cgcgtgcgca aggcattctc actatcatcg atggcgctca cgcaccgggt caaattccgc
     5581 ttcatttgga tgcgcttggt gcagacttct atggtggaaa tttgcataaa tggctttgtg
     5641 ccccgaaagg tgctggcttt ttatatgccc gcccagaggt tcaacatctg ctcaagccct
     5701 tggtggtttc gtggggctac gaagccgagg cgcccggtga ttcgcaattt attgattaca
     5761 acgaatggtg gggcactcgt gatgtctcag cgttccttgc tgtcccgaag gcaattcaat
     5821 ttcaacagga ccaaaactgg gcggaggtaa gagccgcgtg tcatcgcctg gcagtggaga
     5881 cgtggcagcg catccatgac ctgacgggag aaaccccgct tcattccgac cccgaacctt
     5941 ggtttgccca aatggcagtt gccactctgc cagcggattc tgacctcgtg tcccttaagt
     6001 cacgcttgta tgatgaatat cgaatagaga ttccgctcat tgcgtggaat gagaagaaat
     6061 taatccgctt gtctgtgcag ggatat
//
LOCUS       DAOQFT010000068         6020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6557_length_6020_cov_6.140989, whole genome shotgun sequence.
ACCESSION   DAOQFT010000068 DAOQFT010000000
VERSION     DAOQFT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6557_length_6020_cov_6.140989"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..597)
                     /locus_tag="PLR93_02825"
     CDS             complement(76..597)
                     /locus_tag="PLR93_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25921.1"
                     /translation="MSDFFEKVSSQVDPFKKLVSFIPGFSGYIERQNRRDADKLLRET
                     VARRFEEQWTRASSLQVDMVNNGMLSYVDDMEKASLALRTFIDKISTAARGYSGMFDA
                     VKINEKELEAIYQFDAAFFDLADQVKSGLDNVEASMGDEAALPAAIRNITSLSRLAVE
                     TFERRSEIVTGSR"
     gene            778..2196
                     /locus_tag="PLR93_02830"
     CDS             778..2196
                     /locus_tag="PLR93_02830"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNH25922.1"
                     /translation="MSDHVKQLLDLVENSISFDGTTVRVLDAAKFRKNVGKLVERSAL
                     ASDSSAGLARFLVRAAALELGVYAASIHDLYMARGRGDAPMTWSTPAFNLRALSHHAA
                     RAMFRAANKINAGAFIFELARSEMNYTDQRPSEYATNILGAAVAEGYTGPVFIQGDHF
                     QVSAKKYKADAAAELKAVRDLSIEAMAAGFFNIDVDTSTLVDISLPTVQEQQTLNSKL
                     SAELSAFIRENEPAGVTISIGGEIGEVGTENSTEPELRAYMDAYNTNLQKLAPGKPGL
                     SKISVLTGTSHGGTVLPDGSIAEVSIAFDVLRDLSRVARKDYGMGGTVQHGASTVPEE
                     NFNKFVQNEAIEVHLATNFATMFFDNIPADFKKEIYAWLDVNAAGDRKPGMTDEQFYY
                     KVRKNAIGPFKAKSYALPAESLDKLGKAWEAQFDKLFNLLGLKDTKNQTEKFIKPVKV
                     APVLADYVKDAVAAEDVSDLSD"
     gene            2241..2696
                     /locus_tag="PLR93_02835"
     CDS             2241..2696
                     /locus_tag="PLR93_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH25923.1"
                     /translation="MLIPRTAIFVRRGDEYLLLKGAPTKRLWAGKYNGLGGHVDRGED
                     VLSSARRELMEEAGISADLWLAGTVIVDAGEIGILLFVVVGENPQGEVKGSEEGTPEW
                     VQKEAVSQLPVVEDLPVLLERIHSMKKGDPPFAARSFYENDKLNVVFGE"
     gene            complement(2726..3511)
                     /locus_tag="PLR93_02840"
     CDS             complement(2726..3511)
                     /locus_tag="PLR93_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25924.1"
                     /translation="MRQTHQLVITILVLVLLTACGGNEQSSNISLPTVLPPAGQTTSD
                     IVSQLCDNPLFPVKQNASWSYFSSGGPNGDFAYTDTITSLTPDGFVLTSQFPALSLTQ
                     NWICAPEGYIAQQLGGGTTASVSMQNMITDFKTIQVSGVSLPRTLTPGMQWKYDLSMQ
                     GAVAMPGEQTQSPGTFALTMQELGTETITVPAGMFDATKIQATFTANIDVDFEGSTIR
                     YTVNGTSLLWYAPGVGFAKSIENIDFSGTSFTSTTELQSYNIP"
     gene            complement(3517..4683)
                     /locus_tag="PLR93_02845"
     CDS             complement(3517..4683)
                     /locus_tag="PLR93_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH25925.1"
                     /translation="MTTLRTPARIIPRAFPGIKPEEVQEIIVNSQIKSYPAGTILCQE
                     DAIEHTFYMILEGDFEVTKTINNKEKRLLKTLIAGDFFGEMALIHNAPRAATVQSLTP
                     AVVLELDKQGFDRVLKHSPSVATAMVAEISNRLRANDQLAIEDLRLRAREIADAYQKL
                     AEQDMARNEFLTSIAHELRTPLMVASGYLHTLKKGVLTGEQLQSTIEIVSRNVDQIVN
                     LTNDLLFLQEQDLVLRDFQALNLYHVVEGVVEKYQAKAKTRSINIKVRGSANIPSVQG
                     DEPSLERAIAGLVDNAIKFSPYGGTVEIRFADLGNYVSVSVVDYGIGIDPQIRPRIFD
                     RFFHLEKSNDEIYDGLGIGLSITRQVIQQHRGMLDVDSQPGKGSTFTISLPKWK"
     gene            complement(4750..4980)
                     /locus_tag="PLR93_02850"
     CDS             complement(4750..4980)
                     /locus_tag="PLR93_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25926.1"
                     /translation="MNDLEKIIIEEVSTLDDMRLIDVIGFIRYLKAEKPIRQEWIAGW
                     YESAIKSMHEREEELRITPAELEKQLRKKQSQ"
     gene            5181..>6020
                     /locus_tag="PLR93_02855"
     CDS             5181..>6020
                     /locus_tag="PLR93_02855"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH25927.1"
                     /translation="MEKPIERPSILQTYTRSFEPLKNANLRMYLGGQAISLLGTWMQS
                     TAQSWVVWELTQSESALGIVVMLNSLPLFLLAPWAGGFADRFNRRAILMVTQATAMLL
                     AFALAFLVQTKLVQIWHIYVFSFVLGTIAALDFPAQQAFIGDLSGMKQVRQAVTLNIM
                     GLQLARMLGPAAAGFLLKAVGSAAAFWINGASFLVVLFSIFMVKSHQQEIHRRENGRG
                     QFREVLDYVRAQPRMVDLLIFASLVTFFGLSIMNILPAVADDVLKGDSQTFGLLMGAS
                     GAGA"
BASE COUNT         1452 a         1383 c         1487 g         1698 t
ORIGIN      
        1 aatggatgtt gcgtttatat agttgtgcca catatgaata tccgtagggg cggggtgacc
       61 cgcccctaca taaacttaac gactgcctgt cacgatttca gagcggcgtt caaaggtttc
      121 cactgcaagg cgtgagagac tggtgatgtt acgaatggcg gcgggcaggg cggcttcatc
      181 gcccatgctg gcttctacat tatcaagccc gctcttaacc tggtctgcca gatcaaagaa
      241 ggcagcatca aattgataaa tagcttcgag ttctttttcg ttgatcttca cagcatcgaa
      301 catgccagag taaccgcgcg cagcggtgct gatcttatcg ataaaagtac gcaacgccag
      361 tgatgctttt tccatatcgt caacataaga gagcatccca ttgttgacca tgtccacttg
      421 caggcttgaa gcgcgtgtcc actgttcttc aaaacggcgc gctacggttt cgcgaaggag
      481 tttatctgca tcgcgacgat tttgtctttc gatgtaacca ctaaaaccgg gaatgaaaga
      541 caccaacttc ttaaacgggt cgacctgact actaactttt tcaaaaaaat cgctcatgtg
      601 agcctcctgc caaaatatac gtactcggtc aagttttgtt tcacactgaa aaggctgccg
      661 gcaacaaggg gctaaaatga catttatccg gttttattca ggcgcgtcac tgagggtaat
      721 tatatcgttt aattccagtt ataatcaagt tatcaatttt tatttaggag aaatagtatg
      781 tctgaccatg taaaacaatt actcgacctt gtcgagaata gcatttcgtt tgacggaact
      841 acggtccgtg ttttggatgc agctaaattt aggaagaatg ttggcaagtt ggtggaacgt
      901 tctgcgctcg cttcagactc ctccgcgggc ttggctcgtt ttctggtgcg tgctgcggcg
      961 ctcgaactgg gcgtctatgc tgcatcgatc catgacctct atatggctcg cggacgtggc
     1021 gatgccccga tgacttggtc tacgccagcg ttcaacttgc gcgctttgtc tcaccatgcg
     1081 gcgcgtgcca tgttccgcgc agcgaataaa atcaacgctg gtgctttcat ctttgaattg
     1141 gctcgctctg aaatgaatta caccgaccag cgcccgtcgg aatatgcaac caacattctg
     1201 ggcgcggctg tagcagaagg atataccggt ccggtattca ttcaaggtga ccatttccaa
     1261 gtctctgcca agaaatacaa ggctgatgca gctgctgaat tgaaagccgt tcgcgacctt
     1321 tccattgaag caatggcagc tggcttcttc aacattgacg tggatacctc tactcttgta
     1381 gatatcagcc tcccgaccgt tcaggaacag cagaccctta acagcaaact ttctgcagaa
     1441 ctttctgcct tcatccggga aaatgagcct gcaggcgtga ccatctccat tggtggtgaa
     1501 atcggtgaag tgggaaccga aaattccacc gaacctgaac tccgcgctta tatggatgct
     1561 tacaatacca atcttcagaa attggctcct ggcaagcccg gcttgagcaa aatcagtgtg
     1621 ttgaccggta cttctcacgg tggtacggtt ttgcccgacg gctcaatcgc tgaagtcagc
     1681 attgcatttg atgtgttgcg cgatcttagc cgtgttgccc gcaaagatta tggcatgggc
     1741 ggtacggttc aacacggtgc ttcgaccgtt ccagaagaaa actttaacaa gtttgttcag
     1801 aacgaagcga ttgaagttca ccttgcgacc aattttgcga ccatgttctt tgataacatc
     1861 cccgctgatt tcaaaaagga aatttatgct tggttggatg tcaatgctgc tggtgaccgc
     1921 aaacctggta tgacggatga gcagttctat tacaaggttc gtaagaatgc tattggaccg
     1981 ttcaaagcaa agtcttacgc tttgcctgca gagtctttgg ataagttggg aaaggcttgg
     2041 gaagctcagt ttgataagct tttcaacttg cttgggctga aagacaccaa gaaccaaacc
     2101 gagaagttca tcaagccggt gaaggttgct cccgttcttg cagattatgt taaagatgcg
     2161 gtggccgcgg aagacgttag cgatctctca gactaaaaaa gatgcctgtc tctgaacaag
     2221 gcgtaacgaa agatagatat atgctcattc cccgcacggc gattttcgtc cggcgcgggg
     2281 atgagtattt attgctcaaa ggtgcgccca caaaacgatt gtgggctggc aaatataatg
     2341 gcttaggtgg gcatgtagat cgaggagagg atgtactttc ttcggcaaga cgcgagttga
     2401 tggaagaagc tggaatttct gccgaccttt ggctggctgg gacagtgatt gtcgatgcgg
     2461 gagaaattgg tattttgctt tttgttgttg taggggagaa ccctcaaggc gaagtgaaag
     2521 gttcagaaga aggcacacca gaatgggtcc agaaagaggc ggtgagtcaa cttcctgtcg
     2581 tggaggatct gccggtcttg cttgagcgca tccattcaat gaaaaaggga gacccgcctt
     2641 ttgcggcgcg gtctttttat gagaacgata aattgaacgt ggtctttgga gaatgaaaac
     2701 gccgccttat taggcggcgt ttgttttacg gaatgttata actttgcagc tcggtggtgg
     2761 atgtaaatga tgtgccgctg aaatcgatgt tttcaatgga ttttgcgaag cccacgcccg
     2821 gtgcatacca taataaactt gtgccgttga ccgtgtaccg gatggtactt ccttcaaaat
     2881 caacatcgat gttggcagtg aaggtggctt gaatttttgt cgcatcaaac atacctgctg
     2941 ggacagttat ggtttcggta ccgagttctt gcatagtgag cgcgaatgtg cccggggatt
     3001 gagtttgttc accaggcatg gcgactgcgc cttgcatgga aaggtcgtat ttccattgca
     3061 taccgggggt gagtgtgcgt ggcaggctga ctccagagac ttggattgtc ttgaaatcgg
     3121 tgatcatgtt ttgcatggag acgctggctg ttgtgccccc gccaagttgt tgagcaatat
     3181 agccttctgg ggcacaaatc caattttgcg ttagagacag agccgggaat tgtgaggtga
     3241 gtacaaagcc atctggcgta agactggtga tggtgtcggt gtaagcgaaa tcaccattgg
     3301 gtccgccgct gctgaaatag gaccaggatg cattttgttt gacaggaaaa agtggattat
     3361 cacaaagctg acttactata tcggaagtcg tctgcccggc gggtggtagg acggttggta
     3421 acgaaatatt tgaagattgc tcattgcccc cgcatgcagt gagtagaacc agaactagga
     3481 tagtgatgac aagttgatga gtctggcgca tggaatttat ttccatttcg gaagagagat
     3541 tgtgaacgtg cttccttttc ccggctggct gtccacgtct aacatgccgc ggtgttgttg
     3601 aatgacctga cgagtgatcg aaagtccaat ccccaaacca tcgtaaattt cgtcgttact
     3661 tttttcaaga tgaaagaaac ggtcaaaaat acgtggacgg atctgcgggt caatgccgat
     3721 gccataatcc accacgctta cactgacata attgcctaag tctgcaaaac gaatttctac
     3781 tgtgccgcca tatgggctga acttaatggc attatcgact aaccctgcaa ttgcgcgctc
     3841 tagagaaggc tcatcccctt gaaccgatgg gatattggcg cttccacgta ctttgatatt
     3901 tatgctgcga gtctttgctt tggcttgata cttttcaaca acaccctcta caacatgata
     3961 caagttaagt gcttggaaat cacgtagcac aaggtcttgt tcttgcaaga aaagcaaatc
     4021 attggttaag ttcacgatct gatccacgtt gcggctgacg atttcaatag tcgactgcaa
     4081 ctgctcgcca gtgagaaccc ctttttttag agtatgtaaa tatccactgg caaccatcaa
     4141 cggagtccgc agttcgtggg cgatgctggt taggaattca ttacgcgcca tatcttgctc
     4201 agcaagtttt tgatatgcat ccgcgatctc acgagcgcgt aaacgtaaat cttcgatagc
     4261 gagctggtcg ttcgctctta accgattgct gatctctgcc accattgcgg tggctacgct
     4321 gggactgtgt tttaagactc ggtcgaagcc ttgcttatca agttccagca ccacagccgg
     4381 cgttagtgat tgaaccgtgg cggcacgtgg tgcgttatgg attaacgcca tctcgccgaa
     4441 gaagtctcct gcaatcagag tcttaagcag tcttttttct ttattattaa tggtctttgt
     4501 aacttcaaaa tccccttcga ggatcatata aaaagtatgt tcaatcgcat cttcctgaca
     4561 tagaatcgta ccagccgggt aactcttaat ctggctgttg acgataatct cttgcacttc
     4621 ttccggtttg atgccgggaa aagctcgtgg aatgattctg gcaggggtgc gaagtgtggt
     4681 catgcttttg gatatggatc ctacattgat tttagcacgg gcagattatc tgttattcac
     4741 ccattaggac tattgacttt gcttttttct taactgtttt tcaagttctg cgggagtaat
     4801 ccttaattct tcttcgcgct cgtgcataga cttaattgca ctttcatacc agcctgcaat
     4861 ccactcctgc cgaatgggct tctctgcctt gagatagcgg ataaacccga tcacatcgat
     4921 tagacgcata tcatccaagg tggaaacctc ttcaatgata attttttcta ggtcgttcat
     4981 aatagctcct acactttgaa tacaaacagt cattggagaa atatatagga tttcctcgac
     5041 attgttttga aaggaaatcg gtttatagtt aaggggattt cccaaattgt actttgtttt
     5101 attaatgatt ggtaagaatt cagcttggga tttcatccga attcttgtct aaaatctctt
     5161 caatccctta ggagccttcc ttggaaaaac caattgaacg tccttccatt cttcaaactt
     5221 acactcgtag tttcgagccg cttaagaatg ccaatcttcg catgtacttg ggtgggcaag
     5281 cgatttctct gcttggtacg tggatgcaat ctacagcaca aagttgggtg gtttgggagt
     5341 tgactcaatc tgaaagcgcg cttggcatcg tggtcatgct caatagtttg cctctcttct
     5401 tgcttgcgcc atgggcgggt ggctttgcag accgtttcaa tcgccgcgcg attctcatgg
     5461 tcactcaagc caccgccatg cttctagctt ttgccttagc gtttcttgtg caaaccaagc
     5521 ttgttcaaat ctggcatatt tacgtctttt catttgtcct tggcaccatc gccgcgttag
     5581 acttccccgc ccaacaagca tttatcggcg atctctctgg tatgaaacaa gttcgccaag
     5641 cagtgacgct caatatcatg ggcttacaac ttgcgcgtat gttgggtccc gccgcggctg
     5701 gctttttgct gaaagccgtt ggttctgccg cagccttctg gatcaatggc gcgagcttcc
     5761 tcgtggtctt gtttagtatt ttcatggtca agtctcacca gcaggaaatt caccgccgcg
     5821 aaaacggacg tggacaattc cgcgaagtgt tggattacgt ccgcgcacag ccgcgcatgg
     5881 tggatttatt gatcttcgcc tctctcgtga cctttttcgg tctctctatc atgaacattc
     5941 tccccgccgt agccgatgac gtcctcaaag gcgactcgca aacatttggt ctcttaatgg
     6001 gtgcatcagg cgcgggcgca
//
LOCUS       DAOQFT010000069         5964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6664_length_5964_cov_9.522593, whole genome shotgun sequence.
ACCESSION   DAOQFT010000069 DAOQFT010000000
VERSION     DAOQFT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6664_length_5964_cov_9.522593"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1520)
                     /locus_tag="PLR93_02860"
     CDS             complement(165..1520)
                     /locus_tag="PLR93_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25928.1"
                     /translation="MPTQNTIPTVVISNFAEAHIDFLGRSSDPEERDDLINGEDYHGD
                     RTILWADDPKLVIVNYPIAHAEFNVKRLGYSRTSHLAPHEPSHYLCLDILREAHLLEG
                     ILQYAAPNGIVQLIPYATTKEFLQLVEVLRREHHLQVLTPESPDADHFWLRDYIDTKT
                     GFRTLASFWMTDASRFLPFGMICETLKHAAIAAHWFCSRGEACVVKTDTGESGIGTTV
                     ISPEDHLSVEGIFEKLHSQPFYSNELIVVEKYIPSANRISPSAEVKVPKLGEGEPQIT
                     YISNQLFLGFGDFCGIQIDRSIYSEPWCADLKRCTLELAHNLQNMGYVGHFDLDCIVS
                     DDNEVYLLEINARRTGGTHVHEFGKYTFGDDYVSKVSLISFEASNSGTITDAMELLEV
                     IKDFLYPMPGDEPLGMVVTITKPLFKNRFGCIVVAPTSERALQLQAQVDEHIQRYCQS
                     K"
     gene            complement(1529..2614)
                     /locus_tag="PLR93_02865"
     CDS             complement(1529..2614)
                     /locus_tag="PLR93_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25929.1"
                     /translation="MNEAKKSRVFFISLRIKLLIGFTLLFTVVFAVAFYWFYQFSTDL
                     AMQRITDDLVNTIQGATKLIDGDEFQALYTEATPREDGYTDDLRYWAQAKLLWSVKQI
                     EPRAGIYTYIKGTEPNELIFVTSGGALNNPPTGAKFMEYWTTDNVGPNLSGLEELTLQ
                     NTPEGQKNNGCIYGTPGCKVEMYSDDFGSWVSAFAPIKNSKGEVVGAIGVDFKADYVR
                     EVQAAILKKVYIAFAITYISLFFLVFFISSAFTRPILTLTKAAEKIGEGDYKGGLVFL
                     SNVGETSKFPDEIETLDKVFQSMIDKVYTREQSLRKQVEELRIIIDDSKRKKQVDEIV
                     ESEFFQDLKTKAQKIRDNQARKKSDEA"
     gene            complement(2611..3333)
                     /locus_tag="PLR93_02870"
     CDS             complement(2611..3333)
                     /locus_tag="PLR93_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25930.1"
                     /translation="MFEIPELGFEIQEIVNGNTRYLDELIELYLQLFPGYGRYVAVMR
                     RRANRPNDVVPPFLEHQWLALVNGRPAAMTVFKYNYKRNCGLGLDLGVHPDYRNFSYS
                     GYTSLARLMIELRHKQLLMDAKLLGKPLPLGSLVEVESPKVVARFKEYGMLLLPVKYY
                     EPPAPEEALEIISRHELEKTGFNPMHLGIYPIDRDAYNPKDVDQISMCVKAYLMDHYG
                     LSETHWAVQESLQSISIEESAK"
     gene            complement(3333..4679)
                     /locus_tag="PLR93_02875"
     CDS             complement(3333..4679)
                     /locus_tag="PLR93_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNH25931.1"
                     /translation="MNERITNWFGTQHVKLVLALWLLLAINMMVMELSDVVATAGFVS
                     NIGSDSLPWLWLVMTLLTLVGASGYSVLVDRYSRLNLITWLLGVMGVFYLFLMFLFRA
                     QIPDTITFPLLSIITDQQYYILPLAFWALANDVFSVTEGKRVFPLIASGAVVGSLAGN
                     SLAGLSAKLLEQLSAGTEAIFVLAALLLFVSVVVLRIAFFRHHVRARQSKDEDTSLMK
                     SMQVGVEYFTKVAMLKMVAIIMFLSGMALALLEFYFLRSIETTTSGNSLEFQQFLGYF
                     KTAQTIGLLTFQWLITRRLLEKVALKNSFMVLPVFLLSASGIALGLVGVWGVAIGRFF
                     ARMIQRGWDEPTRKSLQGLIPDERRGRVALFMDSVFYNLATIVSSMWMALLLWLMHSA
                     LITELTFQYMALGTALFAALGAMLASFNFNRVYEKSLLDWRFARSKRKSVLDNIEF"
     gene            5078..5839
                     /locus_tag="PLR93_02880"
     CDS             5078..5839
                     /locus_tag="PLR93_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNH25932.1"
                     /translation="MTDEPIKSLARNKIAMLIDGDNAQAGLLSQMLVEAGRYGQVTVR
                     RIYGDWTTQSMNSWKDTLNFHAFQPIQQFRYTIGKNATDSAMIIDAMDILHSDVVDGF
                     CLVSSDSDYTRLATRIRETGVFVMGIGEKKTPKPFVNACDVFVYTENLTSEKKTSPQK
                     NRNVDKTKKAKDEKEELDPMPILSQAFEMAVQQDGWASLANMGNALYQVDPGFDPRTY
                     GHKQLSKMISKLKDRFEMRATEKDGPIIFYVRMKE"
BASE COUNT         1783 a         1473 c         1266 g         1442 t
ORIGIN      
        1 caccaagttc acaatggacg gttcgtcgtc aacgataagt atctttgtca tgatgctatt
       61 ctctcgcggt catttttatt catcataaag gcattgtaaa gatttggtaa agaaaaagcc
      121 acagtgaaca aggtctttca ctgtggcttt ttacaaaaat tttcctattt cgattggcaa
      181 taccgctgaa tatgctcatc tacttgagcc tgaagctgta gcgcgcgctc agatgtaggc
      241 gcaaccacga tacacccaaa tcgatttttg aataatggtt tggtaatggt gactaccatt
      301 cccagcggct cgtctccggg catcgggtag agaaaatcct tgattacctc gagcaattcc
      361 atggcatctg taatcgtacc agagttggaa gcctcaaaac tgatgagaga gaccttactg
      421 acataatcat caccaaaagt atattttcca aattcatgca catgtgtgcc cccagtgcgg
      481 cgggcattga tctcgagcaa atacacttca ttgtcgtcgc ttacgatgca atccaggtca
      541 aaatgaccga catatcccat attttgcagg ttatgtgcca attcaagggt gcaacgtttc
      601 aagtctgcgc accagggttc gctataaatg ctgcgatcaa tctgaatacc gcaaaaatcc
      661 ccaaatccta aaaacaactg attggatata taggtaattt gaggttcacc ctccccaagt
      721 ttgggcacct ttacttcagc cgagggagat attcgatttg ccgaggggat atatttctca
      781 acaacgatca attcgttgga atagaaaggt tgggaatgca gcttttcaaa aataccctca
      841 acagataaat ggtcttcagg agaaatgacc gtcgtaccga taccgctttc cccagtatcg
      901 gtctttacca cgcaagcttc tccacggctg caaaaccaat gcgcggcaat tgcggcgtgc
      961 ttcaaagttt cgcaaatcat gccaaatggc aggaatctac tggcgtccgt catccaaaaa
     1021 gaggccagag tacgaaagcc ggttttggtg tcgatataat cacgcagcca aaaatgatca
     1081 gcatcagggc tttcaggggt taatacctgc aagtggtgtt ccctgcgcaa aacttccacc
     1141 aactgaagaa attccttcgt ggtggcatat gggataagtt gaacaattcc atttggggcc
     1201 gcatactgca ggataccttc aagaagatgt gcttcacgta aaatatcaag gcacagataa
     1261 tgcgaaggtt catgaggagc cagatggctc gtacgtgaat agcccagccg ttttacgttg
     1321 aactctgcat gggcgatcgg ataattgaca attaccaact ttgggtcatc agcccaaaga
     1381 atggtgcgat cgccatggta atcctcacca ttgatcaggt cgtccctctc ttcagggtcg
     1441 ctgctgcgtc cgagaaaatc aatgtgcgcc tcggcaaaat tactgatcac tactgtcgga
     1501 atggtattct gggttggcat ggaacacact aagcttcgtc gctctttttc ctcgcttgat
     1561 tatcacgaat tttctgggcc ttcgtcttga ggtcctggaa gaattcggat tctacaattt
     1621 catccacttg tttctttctc ttactgtcat caatgatgat cctcaattct tcgacctgtt
     1681 tacgcaggct ttgttcacgg gtatagacct tgtcaatcat cgactggaac accttatcaa
     1741 gggtctcaat ctcatctggg aatttggaag tttctcccac attggaaagg aaaaccaaac
     1801 ctcccttgta atcgccttcg ccgatcttct ctgccgcctt ggtaagtgta agaattggac
     1861 gggtaaatgc gctggaaata aaaaagacaa ggaagaacaa ggaaatatag gtgatggcaa
     1921 aggcgatata taccttcttc aagatcgctg cctgcacttc acggacataa tcagctttga
     1981 aatccactcc aatggcacct actacttcac ccttggagtt tttaatcgga gcaaacgccg
     2041 atacccagct tccaaaatcg tctgaataca tttccacctt gcatccaggt gtgccataga
     2101 tacagccgtt gttcttttgg ccttctggcg tattttggag ggtgagttct tccaagccag
     2161 aaagattggg accaacatta tcagtggtcc aatactccat gaatttggca cctgttggag
     2221 ggttattcaa tgcgccgccg cttgtcacga agatcagttc gttgggttcg gttcctttga
     2281 tatacgtgta gatcccagca cggggctcaa tctgcttgac ggaccacaac agtttagctt
     2341 gcgcccagta gcgaaggtca tcagtatatc catcctcacg aggggtcgct tcagtataaa
     2401 gagcttgaaa ctcgtcacca tcaatcaact tagtggcacc ttgaatcgta ttcacaagat
     2461 catctgtgat acgttgcata gccagatctg tggaaaattg ataaaaccaa tagaaggcaa
     2521 cagcaaacac aacagtaaac aataaggtaa aacctatgag cagcttaatc cgtaagctga
     2581 taaaaaacac cctgcttttc ttagcttcgt tcattttgca ctctcctcta tcgaaattga
     2641 ttgaagggat tcctgcacgg cccaatgcgt ttcggataat ccataatgat ccattagata
     2701 agctttgaca cacattgaaa tttgatcaac atctttcgga ttgtaagcgt cccggtcaat
     2761 aggataaatt cccaagtgca tcggattgaa acctgtcttt tcaagctcgt gacggctaat
     2821 aatctcaagg gcttcttctg gagcaggcgg ttcataatac ttgactggca acaacaacat
     2881 gccatactcc ttaaatcgag ccacaacctt cggcgactca acctccacca gtgaccccaa
     2941 aggcaggggc tttccgagca gttttgcatc cattaaaagc tgcttgtgcc gcaattcaat
     3001 cataagcctg gcaagactag tataacctga ataggaaaag tttctataat caggatgcac
     3061 ccccaagtcc aggccgaggc cgcaattcct cttatagttg tatttaaaca cagtcatcgc
     3121 ggcagggcgt ccattgacca acgccagcca ctgatgctct aaaaatggcg gcacaacatc
     3181 attcgggcgg tttgcccgtc gtcgcattac ggcaacgtat cgcccgtacc cgggaaacag
     3241 ttgcagatac aactcgatca gttcatcaag gtaacgagta ttgccattga caatttcctg
     3301 gatctcaaat ccaagctctg gaatctcgaa catcaaaact ctatattatc caaaacgctt
     3361 tttctcttgg agcgtgcaaa acgccaatcc aacaggcttt tttcatacac acggttgaaa
     3421 ttgaaactcg ccaacatggc acccaaagcc gcgaacaggg ctgtgccaag ggccatatac
     3481 tgaaacgtta attccgtgat caacgcagaa tgcatcagcc atagtaagag tgccatccac
     3541 atgctgctaa cgatggtggc aaggttatag aaaacagaat ccatgaaaag ggccacacga
     3601 ccgcggcgct catctgggat caacccctgt aacgacttgc gggtaggttc atcccaacca
     3661 cgttggatca tgcgcgcaaa gaaacgtcca atcgcaacac cccacacccc caccaagcca
     3721 agagcaattc cgcttgcact gagaagaaaa acaggtaaaa ccatgaaaga gtttttcaac
     3781 gcaacttttt ccagcaaacg tcgggtaatc agccattgaa aggttaacaa cccgatggtt
     3841 tgagcagttt tgaagtatcc aagaaactgc tggaattcaa gggaattgcc gctcgtagtt
     3901 gtttcaatgg aacggagaaa ataaaactca agcagcgcca aagccatacc gctcaagaac
     3961 atgataatcg ccaccatttt aagcatggca acttttgtaa aatattcaac acccacctgc
     4021 atacttttca tcaggctggt gtcttcatct ttggactggc gcgcacggac atggtgacgg
     4081 aaaaacgcaa ttcgcaagac gacaaccgaa acaaacaaca aaagtgcagc caggacaaag
     4141 atcgcttcag tcccagctga aagctgctcc aacaatttag ctgacaaacc tgcgagagag
     4201 tttcctgcca aactgccaac cactgcgcca gatgcaatca gaggaaagac tctcttccct
     4261 tcagtaaccg agaaaacatc attggcgagc gcccaaaacg caagcggcaa aatatagtat
     4321 tgctggtcag taattatgga aagtaaagga aaggtgatcg tatcgggtat ctgggcgcgg
     4381 aacaggaaca tcaaaaacag atagaacacc cccatcactc caagcaacca ggtgatcaaa
     4441 ttcaaccttg aatatcgatc cacgagaacc gaatatccgc tggcacccac tagcgtcaaa
     4501 agagtcatca caagccacag ccacggcaaa gaatcggacc caatgttact gacgaaacct
     4561 gccgttgcaa caacatcaga taattccatc accatcatgt taatggcaag caaaagccac
     4621 aaagccagaa ccagtttaac atgctgcgtg ccaaaccagt tggttattct ctcattcaca
     4681 aaaccctcgt cggttaacaa ttttttggat ttgaagcaag ctccaatctc tttttttcaa
     4741 gattcaatga atggatttct ggcgccgtag acaaagcaac ggggacgccc ccgatcttcc
     4801 aaattccatt gaagaacgaa ccctctacaa cttgaaaatg gagcaaagct ctaaaatatt
     4861 ctttcctttt ggggaaaact aacgcgagta tacccgatta tgagaaaggc tccaattctg
     4921 caaaaacgcc tgaatttagt gtaataaatc aggaaaagcc atattacgat ccgaaccttg
     4981 gagtttcttt tttagaccca tacctcttac gctgggtaat cattaagaca agcaaatgtt
     5041 atacaagtga agcctcgtga aagtgtataa taaccttatg accgacgaac ccatcaaatc
     5101 actcgctcga aacaaaattg ccatgctgat cgacggcgac aacgcccagg cggggctgct
     5161 ttcccagatg ctggtggagg caggacgcta cggccaggtg accgtccgcc gcatttatgg
     5221 ggattggacg acccaaagca tgaattcgtg gaaagacacg ctcaattttc atgctttcca
     5281 acctattcaa caattccgct acaccattgg aaagaatgca accgacagcg caatgatcat
     5341 tgacgcgatg gatatcctgc attcggatgt ggtggatggc ttctgtctgg tctccagcga
     5401 cagcgattac acgcggcttg ccacccgtat ccgcgaaaca ggcgttttcg taatgggcat
     5461 cggtgaaaag aagacaccca aacccttcgt caatgcttgt gatgtattcg tctacacgga
     5521 aaacctgacc tcagaaaaga agacctcccc acaaaagaat agaaacgtgg ataaaaccaa
     5581 aaaagccaag gatgaaaagg aagaactgga cccaatgccc attctgagtc aggcttttga
     5641 aatggccgta caacaagatg ggtgggcctc tctggcaaac atgggcaatg cgctctatca
     5701 agtagacccg ggcttcgacc cacgtaccta cggtcacaag caactatcaa agatgatcag
     5761 taaattaaaa gaccgcttcg aaatgcgcgc aactgaaaaa gacggtccga tcatctttta
     5821 cgtacggatg aaggaataaa agcaaaatga cctgtgtcat gcaggtcatt ttgcttttag
     5881 acacaaggtt ttctcgcaaa acccaaaaca gccaaaatgg acagcgacac aaatcaattc
     5941 agccagcgcg aaaaagaagt cata
//
LOCUS       DAOQFT010000070         5928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6741_length_5928_cov_8.789205, whole genome shotgun sequence.
ACCESSION   DAOQFT010000070 DAOQFT010000000
VERSION     DAOQFT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6741_length_5928_cov_8.789205"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..613
                     /locus_tag="PLR93_02885"
     CDS             68..613
                     /locus_tag="PLR93_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH25933.1"
                     /translation="MAGIAVNVAVIQNGKILLTQRDDFETWILPSGGVEDGESLAQAA
                     LRETKEETGLDVALTGLVGTYTRISNMPPIHAVLFTATPIGGTITCQPGETIAVDWFS
                     FDQLPEPLSVGHKRRINDAVSGKKGFVVSQEIIFSSMPDKMTIRDLIELRDKSGFSRQ
                     DFYIKMMEDAILKETVEVMGF"
     gene            769..1785
                     /locus_tag="PLR93_02890"
     CDS             769..1785
                     /locus_tag="PLR93_02890"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNH25934.1"
                     /translation="MYNFLRPLLFRLNPERAHALTLHTLSFAGRFLPSRKVLEGLYQA
                     HSKPVTVFGLTFKNPIGLAAGYDKDGIAIRGLAALGFGHVEVGTVTPKPQPGNPSPRV
                     FRLVEDDAVINRMGFPSRGMEYLEQQLSGGANWNTVLGVNLGKNKETPNEEAVSDYLA
                     LLKKFAAYANYLTINVSSPNTVGLRSLQGREALEGLLTQLHQQRLAEQEKCKKRLPIL
                     VKLAPDLSDAELSDALEAILTTNMDGVIVTNTTLSREGLKSSYREETGGLSGSPLTVK
                     SEAVLRQTLKFVNGKIPVVSAGGVMNSEDAKRRLDMGAALVQVYTGLVYRGPGLVKEI
                     VRAL"
     gene            complement(1782..3845)
                     /locus_tag="PLR93_02895"
     CDS             complement(1782..3845)
                     /locus_tag="PLR93_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing serine/threonine
                     protein kinase"
                     /protein_id="HNH25935.1"
                     /translation="MAIPEKIGRYEIKEELGRGGFATVYRAYDPRFEREVAIKFLPSE
                     LMHSDPQFRMRFQREAKIIAQLEHPSIVPVYDVGAENEQPYFVMRYMSGGSLSERIKA
                     RTYTIEEAVKIIENLAPGLDEAHAKNIVHRDLKPANILFTEKNVPLIADFGIAKFTQG
                     DSTVDNMTGSAIIGTPAYMAPEQASGEAVDGRVDIYALGVILYEMVTGKQPYTADTPL
                     GLAIKHVTEPVPRILEANPNLPVWMEKVISTAMAKNPDDRFYSAVELVETIKAFLRGE
                     NPQINANVTKKMSPYNSTATFKRTKTAEPKRRNWVVPALVSVGFLAVAGVCAFALVSS
                     GMLQSLLEPVVTPTIAATSTISPATEVTETSEAITATVEVPPTENVPTLPLMGGADKI
                     AFIKNNDIWVMNVDGSELKQHTNDGLPKFNLQWMPDGKNLLYMTGKTVKTVNIDTEVE
                     EVLMNFVSAEYFEGFSVSPNGKQAAISINRELFIVPFEIETLRAATRKSALLEMKGCF
                     YNDLGIKELQWSDDSTKLAVEFIANSNGSFADAIRVYDVQDCEKISNSKIDEFPAGRF
                     QFSGDLSSFDWDGDLLFFLTSNTRNGGFGDLALYNIFTHKAEKAAPYESNCCYRDAAF
                     SPDGTQVIFAFQDIRLAGNSPINLYIIPADSISTPRKLEPILPMDFFTKRDDAPQPVM
                     RAAQP"
     gene            complement(3871..4500)
                     /locus_tag="PLR93_02900"
     CDS             complement(3871..4500)
                     /locus_tag="PLR93_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25936.1"
                     /translation="MSKLTPKIILEGTRLTFKTDIAFALNEHPRIVGPRKYKYHSPLV
                     SGEWCAFTNFPWGRGLINFEPQEEALAMETYDTWVKLFELQKYYSWIVDRFHLSTRMY
                     QLMTYQKDYDFRWLEERLLPLNFRLVLMTRSPESFAAARAERLKVSGNPSQYDDLNLF
                     VEEQKMIRKLFNDSILPKIEIDISDNNISAAVEHIADWMESTGGLYMAE"
     gene            complement(4497..5372)
                     /locus_tag="PLR93_02905"
     CDS             complement(4497..5372)
                     /locus_tag="PLR93_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH25937.1"
                     /translation="MMKKLAFLSLLLVSSLACATIEKALTSGATPTPIVIPTIAPSQT
                     AVPIATDIIRKEESDIFCPSNVTEAVDAYNKGLTYENAGDTASAEKAYRDAIALDPNY
                     CDAMDNLALLVRKNGNSDEAIALYENSIKVAPDNQVAHLGLANTYMELEEYDKALAEY
                     NAILAINPENPEGYYGAGRVYFSKEDFKSAIPQFKQAEELYKAEGSSYLIDAQVYLGF
                     SYALIDEFETGRDYLEMVYPQMQDNGYVNYFLGYCYYYGTSIRDEKLAKQYLLRARDL
                     GIDLGSDLETFVNTP"
     gene            5515..5880
                     /locus_tag="PLR93_02910"
     CDS             5515..5880
                     /locus_tag="PLR93_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25938.1"
                     /translation="MDDKITIIEGPPPVFEHVNDGWAMGLNESPNLSIPALTRLRTFN
                     GPALVQRCYNAWHNKTSIHLHYRNETGLEQTAPILAARNVETPEGHVLLLWVYLDSEK
                     VEYETDDASDDDLDDYPGE"
BASE COUNT         1523 a         1319 c         1491 g         1595 t
ORIGIN      
        1 tgccaagcga attcaaaaga tgatggatat gttgaaacag ggcaaagaaa attaagaggt
       61 acaaaacatg gccggtattg ccgtaaatgt cgcggtaatt caaaatggaa aaattctctt
      121 aacacaacgt gatgattttg agacttggat attacccagt ggtggggtcg aagatgggga
      181 aagccttgcg caagctgcgc tccgtgagac aaaagaagaa actgggctcg atgtagctct
      241 tacagggtta gttggaacct atacaagaat aagtaatatg ccgcccattc atgctgtttt
      301 gtttacagct acacctattg gagggacaat tacctgccag cccggtgaga cgattgcagt
      361 ggattggttt tcttttgacc aactccctga acctctttct gttggacata aaagaagaat
      421 aaatgatgcc gtttctggaa aaaaagggtt tgttgtttct caagaaatta tattttcttc
      481 catgccagat aaaatgacga taagagattt gattgaactt cgtgataaat cgggcttttc
      541 acgacaagat ttttatataa aaatgatgga agatgcaata ttgaaagaga cagttgaagt
      601 tatgggattc tgaggaattc agaaaatgat taagctttat tcaaaaatat ttttagcctt
      661 gattacattg ttgtctttgg cttgtaatct tttatctatt ccgcaaaaga cggaaactgt
      721 aaacaccgaa cctgttaaga acgaacagac tcctaacatt ctataactat gtataacttt
      781 cttcgtccac ttctctttcg cctcaaccca gaacgcgcac atgcgttgac cctgcatact
      841 ttaagcttcg ctggaagatt tcttccttca cgtaaagtgc ttgaggggtt gtaccaagcc
      901 cattccaaac cagtcactgt gtttgggttg acgttcaaga atccgattgg gttggcggca
      961 ggatacgata aagatggcat cgccattcgc ggacttgctg ctctagggtt tggtcacgta
     1021 gaggtgggca cggtcacccc caagccgcaa ccgggaaacc ccagcccgcg ggtgtttcgt
     1081 ttggtggaag atgatgcggt catcaaccgc atgggcttcc cttcacgtgg gatggaatat
     1141 ttggaacaac agctttctgg tggcgcaaat tggaatacgg ttcttggagt taaccttggc
     1201 aagaacaaag agactcccaa cgaagaagcg gtctctgatt atttggcatt gttgaaaaag
     1261 tttgccgcat atgccaatta tctgacgatt aacgtcagct cgccaaatac ggtgggactt
     1321 cgcagcttgc agggacgtga agccttggag gggttgctca cgcaattaca tcaacagcgt
     1381 ttggcggaac aagaaaagtg taagaagcgt ttgcccattc ttgtgaagtt ggcgccagac
     1441 ctttctgatg ctgaattgag cgatgccttg gaagctatcc tcaccacgaa catggatggt
     1501 gtgatcgtca cgaacaccac attgagtcgt gagggattaa agtcaagtta tcgggaagaa
     1561 acaggcgggt tgagtggcag tccgcttacg gttaagagcg aggcggttct tcgtcagacc
     1621 ttgaagtttg tgaatgggaa aatcccggtg gtgagtgcgg gcggagttat gaattctgag
     1681 gatgcaaaac gccgcctcga tatgggggcg gcgctggtgc aggtctatac ggggttggtc
     1741 tatcgcggac ccggtttggt gaaagagatc gttcgcgctc tttagggttg tgcagcgcgc
     1801 atgacaggtt gtggggcatc gtctcgtttg gtgaagaaat ccatgggaag gatgggctcg
     1861 agtttgcgcg gcgtggaaat tgagtcagcg ggaatgatat agagattgat tggactattg
     1921 cccgccagac gaatatcttg aaaggcaaag atcacttggg tgccatcggg gctgaaggca
     1981 gcatcgcgat agcaacaatt gctttcatac ggcgcggctt tttcggcttt gtgggtgaag
     2041 atgttgtata acgccagatc tccgaacccg ccattgcgtg tgttgctggt gaggaagaag
     2101 agcaggtcac catcccaatc gaaactggag aggtcgcctg agaattggaa acgcccagcg
     2161 gggaattcat cgattttgga gttgctaatc ttttcgcaat cttgcacatc atacacacga
     2221 atggcatcgg caaaactacc gttggaattc gcaatgaact caactgccag cttggtgctg
     2281 tcatcagacc attgaagttc tttgataccg aggtcgttgt aaaaacaacc tttcatttca
     2341 agtaaagcgc ttttgcgagt tgcggcacgc agtgtttcaa tctcgaatgg cacaatgaat
     2401 agttcacggt tgatgctaat ggctgcttgt ttgccattgg gcgaaacact gaaaccttcg
     2461 aagtattctg ctgaaacaaa gttcatcaat acttcttcta cttctgtatc aatgttcacc
     2521 gtcttcaccg ttttaccggt catataaaga aggttcttgc catctggcat ccattgcaaa
     2581 ttaaactttg gcaagccatc gttggtatgt tgttttaatt cactgccatc tacattcatc
     2641 acccaaatat cattgttctt gatgaatgca atcttatcgg ctccgcccat caaaggcagg
     2701 gtcggcacat tttcggttgg gggcacttca acagttgctg tgatggcttc ggatgtttca
     2761 gtcacttcag tagcaggtga gattgtagat gtggcagcaa tggtcggagt aacaaccggc
     2821 tctaacaaag actgaagcat gccgctgcta acgagggcaa aagcacatac gccggcaact
     2881 gcgagaaacc caactgacac caaggcaggg acgacccaat tgcggcgttt gggttctgcg
     2941 gttttggttc gtttgaaagt cgccgtgctg ttataaggcg acattttctt ggtcacattg
     3001 gcattaatct gcggattctc tccgcgtaga aatgccttga tggtttcgac caattccact
     3061 gcagaataga agcggtcatc tgggtttttc gccatcgccg tagagatgac cttttccatc
     3121 cagaccggta gattcgggtt ggcttccaaa atacgcggca caggttcagt gacgtgtttg
     3181 attgccagtc caagtggagt gtcggcagtg tagggctgtt tgccagtgac catttcatac
     3241 aagattacgc ccaaggcata aatatccaca cgtccatcca cagcttcgcc agaggcttgc
     3301 tcgggtgcca tgtacgctgg ggtgccaatg atggcagagc cggtcatgtt atcgaccgtg
     3361 gagtcacctt gagtgaattt tgcaatgccg aaatcggcaa tcaatgggac gttcttttcg
     3421 gtaaaaagaa tattggctgg cttcaggtcg cgatggacaa tgttcttggc atgcgcttca
     3481 tccaaccctg gcgcgagatt ttcaatgatc ttgacggctt cttcaatggt gtaggtccgc
     3541 gctttgatgc gttcagagag tgaccctccg ctcatataac gcattacaaa ataaggctgt
     3601 tcattttcag cacctacatc gtagaccggc acaatcgagg gatgttccag ttgggcgatg
     3661 atcttcgcct cacgctggaa acgcatgcgg aattgtgggt ccgaatgcat cagttcagat
     3721 ggcagaaact tgatcgccac ctcgcgttcg aagcgcgggt cgtacgcccg atagaccgtg
     3781 gcaaacccac cacgcccgag ttcctctttg atttcgtaac gcccgatctt ctctggaata
     3841 gccatgcgag caagtatacc gatgaagtct ttattctgcc atgtacaacc cgcccgtgga
     3901 ttccatccaa tcggcgatgt gttctacggc agccgaaatg ttgttatctg agatatcgat
     3961 ctcgattttg ggcaggatcg aatcattgaa caacttgcgg atcatcttct gctcttccac
     4021 aaagagattc aagtcatcat attgagaggg gtttccagag accttcaagc gttcggctcg
     4081 agctgcggca aaagattcgg gtgagcgcgt catcaagacg aggcgaaaat tgagcgggag
     4141 gagacgttct tccagccagc ggaagtcata atctttttga tacgtcatga gttgatacat
     4201 gcgtgtggaa agatgaaaac ggtccacgat ccatgaatag tatttttgca attcaaagag
     4261 tttgacccag gtatcgtagg tttccattgc caatgcttct tcttgtggct cgaagttaat
     4321 gagtccgcgc ccccagggaa aattggtaaa agcgcaccat tctccagaaa ccagcggcga
     4381 gtgatactta tacttgcgcg gaccgacaat gcgcggatgt tcgttgagtg caaacgcaat
     4441 atcggttttg aaggtcaggc gtgtcccttc gaggatgatc ttcggtgtga gtttgctcat
     4501 ggtgtgttga cgaaagtctc caagtctgat ccaaggtcta tgccgaggtc gcgggcacgc
     4561 agcaggtatt gttttgccaa cttctcgtca cgaatggatg tgccataata ataacagtag
     4621 ccaaggaagt agttaacata gccgttatct tgcatttgcg ggtaaaccat ttcaagataa
     4681 tcgcgcccgg tttcgaattc atcgatcaat gcgtatgaaa aaccgagata aacctgtgca
     4741 tcgataagat agctggaccc ttcggcttta taaagctctt ctgcttgttt aaactgagga
     4801 atagcagact tgaaatcttc tttggagaaa tacaccctgc ctgccccgta atagccttct
     4861 gggttttctg ggttgatcgc caagatcgca ttgtattcag ccaatgcttt atcgtattct
     4921 tcaagttcca tgtaggtgtt tgccaaccct aagtgcgcta cctgattatc gggtgctact
     4981 ttgatggagt tttcatacaa agcaatcgcc tcatctgaat ttccattttt gcgtaccaaa
     5041 agagccaggt tatccatggc atcgcaataa ttcgggtcca atgctatggc atctcgatag
     5101 gctttttctg ccgaggcagt atcgcctgcg ttttcatagg tcaacccttt gttgtaggcg
     5161 tccacagctt ctgtcacatt ggatggacaa aaaatatcag attcttcttt tcgaatgata
     5221 tcggtggcaa tgggcaccgc agtttgggat ggggcaatgg tagggatcac aatgggtgtg
     5281 ggcgttgcgc cggaagtcaa cgctttttca atcgtggcac atgccagcga acttaccaaa
     5341 agaagagata aaaaagccaa ttttttcatc atttgcctcc agtttgtatg tgaaaattat
     5401 ctcattcaat gcacccagaa cctatagtct atttgtcctg atttcgattc tgataaattt
     5461 ctgaaacgat tcgacaaccc agttgtggta tattcccgcc aaggtaaatt tattatggac
     5521 gacaaaatta cgatcataga aggtccgccc cccgtttttg agcacgtcaa cgacggatgg
     5581 gcaatgggct tgaacgaaag cccgaatctc tccatccccg cgcttacgcg tctgcgcacg
     5641 tttaatggtc ccgccttggt gcagcgctgt tataacgcgt ggcataacaa aacttccatc
     5701 cacttgcatt accgcaacga aacgggcttg gaacaaaccg ctcccattct tgcagcccgc
     5761 aatgtggaaa cccccgaagg gcacgtcttg ttgttgtggg tctatctcga tagcgaaaag
     5821 gtcgaatacg aaaccgacga cgccagcgac gacgatttag acgattaccc cggcgaataa
     5881 agttgattac aatcagtgac agtcatcaag aaggtgactg tcactttt
//
LOCUS       DAOQFT010000071         5926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6748_length_5926_cov_6.749957, whole genome shotgun sequence.
ACCESSION   DAOQFT010000071 DAOQFT010000000
VERSION     DAOQFT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6748_length_5926_cov_6.749957"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLR93_02915"
     CDS             <1..390
                     /locus_tag="PLR93_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNH25939.1"
                     /translation="LRFELSAYQTRLTDLISSLLADHQASVSALAARLKYVSPERRIQ
                     AEYQHIDELSRRAVSALTHRIQLQSARVDGISKRLGALNPAGILSRGYAIITREADGK
                     VVSQVLQAQGDMTVRVSDGEFTVKRNS"
     gene            426..671
                     /gene="xseB"
                     /locus_tag="PLR93_02920"
     CDS             426..671
                     /gene="xseB"
                     /locus_tag="PLR93_02920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNH25940.1"
                     /translation="MAKAKPTQKPVEELTYEEALAELEQTVELLEGEQNPLDDAMKLF
                     ERGQALAARCGVLLEAAQLKVQKLVGETTLDFEEEDE"
     gene            668..1120
                     /locus_tag="PLR93_02925"
     CDS             668..1120
                     /locus_tag="PLR93_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNH25941.1"
                     /translation="MNLLAIFQNKVLIAVLLAWLLAQAMKIPTEYLRSRRWMWAMFFA
                     AGGMPSSHTALMVSGTLAVGLYYGFDNPIFGVAVAATMIIAHDAAGVRRQAGKHAERI
                     NVLFDELLRGHTWSENDLKEVIGHTPLEVVGGIIFGMLVAIVQWMVWK"
     gene            complement(1182..2765)
                     /gene="murJ"
                     /locus_tag="PLR93_02930"
     CDS             complement(1182..2765)
                     /gene="murJ"
                     /locus_tag="PLR93_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNH25942.1"
                     /translation="MTETKSNANRQIARAAGTVMFAFVLSNIMGLVRQILVSKTFGTN
                     QSLDAFYAAATFPDLLFSLLAGGALASAFVPTFTGFLANEKKEDAWRLASAVTNLVIL
                     LLSLVSGLAALFAPGIVRHVLFVLKPELDPSLQSLTVELLRIILIAPTIFGVSGLLMS
                     ILNTHQKFWLPALAPVFNWVGWILGIIFFVPSMGIYGLAWGYVIGAMLHLLVQVPGLL
                     KLDRVRYSFTLGIGNPAIKEVARLMAPRLLGVAAVQINFVINAMIASGQGEGSLSAIN
                     VARMLMTMPLFVIAQAIATAALPTFSAQVARGEMSEMRSSLAATLRGVVLLSLPATVG
                     LILLRQPVIVLLFQRGEFDATSTQMVSWALLWYTAGLVGHAVVEILSRAYYALHDTKT
                     PVIVGTIAMGLNALFSFGFAWLFSQAGWMPHGGLALANSFATALEAVALFIIMRKRLD
                     GIEGGSILRGVSASAIAAIAMGLAVVAWMAIGANLSLWVSTPVGIAIGGLVYFGALFF
                     LRVPELNALVGAVMRRIRR"
     gene            2790..3908
                     /locus_tag="PLR93_02935"
     CDS             2790..3908
                     /locus_tag="PLR93_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNH25943.1"
                     /translation="MKKTSTSLFLLVILSLACSSTTLTPDVAPQTPPTVDSTDPASAT
                     LTEIPLPVGYGAKNSWIEIYFTDPSNSLSSQKTGGIDQPLADAIDSAKLTVDVAIYSL
                     SLNSIRDALLRAQDRGVQVRMVMESENLDRSDPQRLLDAGIPILGDRREGLMHNKFTV
                     IDGSEVWVGGMNYTDSGTYEDNNVLMRIRSTKIAENYTKEFEEMFVDDKFGDNVLAET
                     PNPRVTMDGTPVDTYFSPDDGVQSILLDILNEAQSSIYFMAFSFTSDELGDAILQRAE
                     SGVTVAGVMDEEQVQSNQGTEYDAFRRAGLDVYIDGNPGQMHHKIMIVDESIVIVGSY
                     NFTNSAETRNDENLMVIYDKDIAAQFLNEFQRVYGQAK"
     gene            4032..4646
                     /locus_tag="PLR93_02940"
     CDS             4032..4646
                     /locus_tag="PLR93_02940"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNH25944.1"
                     /translation="MKKQSYIFLVLVALIAVFVSACGGAAAATDTSAGSDKPRASLVE
                     FTGVIEAIDGNQWTVNGQVITVDPTVLRDGPFVVGDTVKVEAEVQSDGSVVITRVEPP
                     VVAVGTDDISNDNSNANINDDNSNASLGNANGNLNDNTSLGSGIGNSNGNSNESLSNG
                     NTNDDDHGGNSNGDDDDDNGNSNGNSNGDDDDDHGGNSNGSDDH"
     gene            4725..>5926
                     /locus_tag="PLR93_02945"
     CDS             4725..>5926
                     /locus_tag="PLR93_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25945.1"
                     /translation="MQKTLRTSLLLFIAFFIGMAVIQFSTPNLPDNDGFYHIKMAWLM
                     RTEGLTPEFIWLPKSILNQNDFYDHHFLYHVALIPFTFGDLRVGAKLASVIFSALAFL
                     AVWVLFDRQKIPFAWLWALALLGISDAFLFRMSIPRAQSLSLGILALGLLWMFEGKYK
                     HLAVLSFFYVWTYNAFPLMIALAGLYTLAILLTEKRLDLRPVLWTSFGIFAGLVLNPY
                     FPKNLTFTYHHFLSKLQLGESIKVGNEWYPYDTAQVLSNSLLALVALFSGSMALGFSG
                     RKMTVQTVTAFLIALFFGIMMFNARRFVEYFPPFALIFSAFAWAPLFDDDGQPTQSWM
                     QKIPAGILALAILAGMVKSIPAVQHQLGTSKPYDLYAGASFWLLQNSERGELIFQTDW
                     DDFPRLFY"
BASE COUNT         1546 a         1444 c         1462 g         1474 t
ORIGIN      
        1 cttcgctttg aactttctgc ctaccaaacc cgcctcacag acttaatctc tagtctgctc
       61 gctgaccatc aagcttccgt ctccgccctt gccgcgcggc tcaagtatgt ctctcccgaa
      121 cggcgcatcc aagctgaata tcagcacatc gacgaactct cacgtcgggc tgtttctgcg
      181 ctgactcacc gcatccaatt gcagtctgcc cgcgtagatg gaatatccaa acggctggga
      241 gctttgaatc ctgccggcat cctctcgcgt ggatatgcta tcatcacccg cgaggcagat
      301 ggcaaagtcg ttagccaggt cttgcaagcg caaggcgata tgaccgtccg tgtgagcgat
      361 ggcgaattta cagtaaagcg caattcgtag tctacggatt acacgaatct ctttttggag
      421 caatcatggc aaaagcaaaa cccacccaaa aacctgtcga agagctaacg tatgaagaag
      481 ctctcgccga acttgagcag accgtggaac tgttggaagg ggagcagaac ccattggacg
      541 atgcaatgaa gctattcgaa cgcgggcaag ctcttgccgc ccgctgtggt gttttgctgg
      601 aagccgctca acttaaagta caaaaactag tcggagagac aactttagat tttgaagagg
      661 aagatgaatg aatcttttag ctatttttca gaacaaagta ttgatcgctg tgttgttggc
      721 ttggttgttg gcgcaagcga tgaaaatccc cacagaatat ctgcgctccc gccgttggat
      781 gtgggcaatg ttctttgccg caggtggaat gccatcttcc cataccgctc tgatggtatc
      841 tggcacactg gcagtgggtc tgtattacgg ctttgacaac ccgatcttcg gtgtggctgt
      901 ggctgcaacc atgatcattg cgcacgatgc tgctggtgtc cgtaggcaag caggcaaaca
      961 tgccgagcgc atcaatgtgc tatttgatga gttactacgt ggacatacat ggagcgaaaa
     1021 cgacctcaag gaagttatcg gtcatacgcc attggaagtg gtgggcggca ttatctttgg
     1081 catgttggtt gccattgtgc aatggatggt ctggaagtaa aaataaaatt cctccttgaa
     1141 aattttcatt cctctttatc cagataaaga ggaatgattt tttaacgccg aattcgacgc
     1201 atcaccgcgc caacaagtgc attcaattca gggacacgca aaaagaacaa tgctccgaaa
     1261 taaactaacc cgccaatggc aatgccaacc ggagtggaga cccataaact aaggttggca
     1321 ccgattgcca tccatgccac aacggcaaga cccatggcaa tggcggcaat ggcagaggca
     1381 ctcacaccgc gcagaatgga gccgccttca ataccatcca aacgtttgcg catgataatg
     1441 aagagagcca cagcttcaag cgccgttgcg aaggaatttg ccaacgccag accgccgtgg
     1501 ggcatccaac cggcttgaga gaaaagccag gcaaagccaa aactgaataa agcattcaac
     1561 cccattgcaa tcgtcccaac tatgacggga gtctttgtat cgtgcaaagc gtaataggca
     1621 cgcgagagaa tctctacaac ggcatgaccc accaaacccg ccgtgtacca aagcaacgcc
     1681 cacgaaacca tttgggtcga ggtcgcatcg aactcgccgc gctgaaagag caaaacaatc
     1741 accggctggc gtaacagaat caacccaacg gtggcaggca atgaaagcaa tacaacaccg
     1801 cgcagggttg cggctaacga agaacgcatt tcgctcatct caccgcgagc cacttgagcc
     1861 gagaaggtgg gtaaggcggc agttgcaatc gcctgagcaa tgacgaacag cggcatggtc
     1921 atcaacatgc gggcgacatt gatggcagac aggcttcctt ctccttgacc tgatgcgatc
     1981 atggcattga tcacaaaatt gatctgaacc gctgccacac ctaataaacg cggcgccatg
     2041 agtcttgcta cttctttaat agcggggtta ccaattccta atgtgaagga atatcgcaca
     2101 cgatctaact tcaataaccc tggcacttgc accaataaat gaagcattgc accgataacg
     2161 tatccccatg ccaaaccgta aatgcccatc gaagggacga aaaagataat gccgagaatc
     2221 cagccaaccc agttaaagac cggtgcgagc gcaggcagcc aaaatttctg atgtgtgttc
     2281 aggatgctca tcaacaatcc gctcacgccg aagatggttg gcgcgatgag aatgatgcga
     2341 agcagttcca ctgtcagcga ttggagggaa gggtcaagct cgggcttgag aacgaataga
     2401 acatggcgaa caattcccgg cgcgaagaga gccgcgagtc cgcttacgag gctgagtaac
     2461 agaatcacca gattcgtcac ggcagaggcg agccgccagg catcctcttt tttttcattc
     2521 gcaagaaaac cagtaaaagt tggcacgaag gctgaagcca atgccccgcc tgctaacaaa
     2581 ctaaaaagca ggtcagggaa agtggctgcg gcatagaagg catcgaggct ttgattggta
     2641 ccgaaagttt tcgaaacgag gatctgacgc accagcccca taatgttgct cagcacaaaa
     2701 gcgaacatca ccgtccctgc ggcgcgtgcg atctggcgat tggcgtttga tttggtttct
     2761 gtcatggcgg caagattata atgtttttca tgaagaaaac atcgacaagc ctctttctcc
     2821 ttgtgatcct ttcactggcg tgcagttcta ctactctcac gccagacgtt gcgcctcaaa
     2881 ccccgccgac tgttgactct acagacccgg catctgccac actgacggag attcccttac
     2941 cagttgggta tggcgccaaa aattcgtgga tcgaaattta tttcaccgac ccatcgaatt
     3001 cattatcgtc tcaaaagact ggcggcattg accaaccctt ggcggatgcc atcgactctg
     3061 ccaagttgac ggtagatgtt gcgatttata gtttgagtct gaatagcatt cgtgatgcgt
     3121 tgttgcgtgc gcaagatcgt ggcgtgcaag tgcgcatggt gatggaaagc gagaacctcg
     3181 accgttctga cccgcaacgt ttgcttgatg cgggcatacc tattttggga gatcgccgcg
     3241 aaggcttgat gcacaataag tttacggtca ttgatggttc tgaggtatgg gtggggggca
     3301 tgaactatac cgactcagga acatacgaag ataacaacgt gctgatgcgc atccgctcga
     3361 caaagatcgc tgaaaattac accaaagaat tcgaagaaat gttcgtggat gataaatttg
     3421 gcgataatgt ccttgccgaa acgcccaatc cgcgtgtgac gatggatggt acgccagtgg
     3481 atacgtattt ctcgcccgat gatggtgtgc aatctatttt gctcgatatt ctcaatgaag
     3541 cacaatccag catttacttt atggcgtttt cttttacatc tgatgaatta ggcgatgcca
     3601 tccttcagcg cgcagaatcc ggcgtcactg tagcaggggt gatggacgag gaacaagtcc
     3661 agtcgaatca aggaaccgag tacgatgcct tccgtcgagc cgggttggat gtgtatatcg
     3721 acggcaaccc cgggcagatg catcacaaaa tcatgatcgt ggacgaaagc atcgtgattg
     3781 tcggttcgta caattttacg aatagcgccg aaactagaaa cgatgagaat ttgatggtga
     3841 tctacgataa agacattgcg gctcagttct taaatgaatt tcagcgagtg tatggtcagg
     3901 caaaatagac tttaacctgt attttattct ttacggctcg tccctaaaat tgcacagggg
     3961 cgagccgtaa cttttatgta ccctctacga ctatctgggt gtagccaaat aaaaaaagaa
     4021 gaggtgtaaa catgaaaaag caatcttata tcttcctggt tctggtagcg ttgatcgcag
     4081 tttttgtcag tgcctgtggt ggggcagccg ccgccaccga tacatcggcg ggttcagaca
     4141 agccgcgtgc gtcactagtt gaatttaccg gcgtgatcga agccatagat ggcaaccagt
     4201 ggaccgtcaa cggacaggtc atcaccgtgg accccacagt tctgcgcgat ggtcctttcg
     4261 tagtgggtga taccgtcaaa gtagaagctg aagtacagtc tgatggttct gtggtgatca
     4321 cccgtgtgga acccccggtt gtggctgtag gtaccgatga tatttccaat gacaattcca
     4381 atgcgaatat caacgacgat aacagcaatg ccagccttgg aaatgccaac ggtaacttaa
     4441 acgacaatac cagccttggt agcggcatcg gaaactccaa tggcaattcg aacgagagtc
     4501 tgagcaatgg caatacaaat gatgatgacc atggcggtaa ctcaaacggc gatgatgacg
     4561 atgataatgg caactcgaat ggcaacagca atggcgacga tgatgacgac catggcggca
     4621 attcgaacgg tagtgatgac cattagtttt cactagccaa ggaatagaaa agtcgcccgg
     4681 gagaaacctg ccgggcgact tggcatctta ctaaaaagac ctctatgcaa aaaacacttc
     4741 gaacttcact gcttttattc attgcttttt ttattggcat ggcggttatt caattttcca
     4801 cgccaaacct gccagataac gatggcttct accatatcaa aatggcttgg ctcatgcgca
     4861 ccgaaggctt gactcccgag tttatctggc ttcctaaaag catcttaaac cagaacgatt
     4921 tttacgatca ccattttctc tatcatgtag ctcttatccc ttttacattt ggtgatctgc
     4981 gcgtgggtgc caaacttgca agtgttatat tttctgcgct cgcttttctt gctgtgtggg
     5041 tattgtttga tcgccagaaa attccttttg catggttgtg ggcgctggct ttgttgggca
     5101 tttcagatgc ttttttgttt cgcatgtcca ttccgcgggc gcaatccctt tcactcggta
     5161 tcttggcact cggtctgtta tggatgtttg aaggcaaata taaacatctg gctgtgcttt
     5221 ccttttttta tgtttggacg tacaacgctt tcccattgat gattgcttta gctggtttgt
     5281 acacccttgc tattcttttg acggaaaaac ggctcgacct ccgcccggtc ttgtggacca
     5341 gcttcggcat tttcgccggg ttggtgctca acccctactt tcccaagaac ctgaccttta
     5401 cctatcatca tttcttgtct aagttgcagt taggtgaatc gatcaaggtt ggcaatgagt
     5461 ggtatccata cgatacagcc caagtattga gcaattcctt attggcactg gtcgccttgt
     5521 ttagtggaag tatggcgcta gggttttcag gtcgcaaaat gacggtacaa acagtaactg
     5581 catttttgat tgctttattc ttcggaatca tgatgttcaa tgctcgtcgc tttgtggagt
     5641 acttcccgcc atttgccttg atattttctg cattcgcttg ggcgccgctc tttgatgatg
     5701 atggtcaacc gactcaaagc tggatgcaaa agattccagc cgggattctg gctctcgcaa
     5761 tccttgctgg catggtgaaa tcgattcctg cggtacagca ccaattggga acatccaaac
     5821 cctatgacct gtatgcaggc gcatcttttt ggctgttaca gaattcagaa agaggagaac
     5881 tgatctttca aaccgattgg gatgattttc cgcgcttgtt ctatca
//
LOCUS       DAOQFT010000072         5893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6795_length_5893_cov_5.601747, whole genome shotgun sequence.
ACCESSION   DAOQFT010000072 DAOQFT010000000
VERSION     DAOQFT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6795_length_5893_cov_5.601747"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLR93_02950"
     CDS             <1..278
                     /locus_tag="PLR93_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25946.1"
                     /translation="DGVIAAHISNRHLDLQPVFWQLAQEYGLKMVRIANEAGAEEEFP
                     SEWLLLTSNEDILNIPQIHERALGYEGYSTSIPIWTDDYSNLFQILR"
     gene            complement(341..982)
                     /locus_tag="PLR93_02955"
     CDS             complement(341..982)
                     /locus_tag="PLR93_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNH25947.1"
                     /translation="MLKKLREPVNSLTHWGGAVLALIGLIALLIVGWSTPAKVISLVV
                     YGISLIFMFSASATYHMVQVKDKALEIFRKIDHSAIYALIAGTYTPFCINAFEGFWKW
                     GMLSIIWSLVVVGIVVKVFYIRAPRWLNAGIYVIMGWFSVSAAGQMLAALPAWVLTWL
                     IIGGVIYTLGAIVYITKIFNFKPGVFGFHEVWHIFVLLAAAAHFVAVMGVALY"
     gene            complement(985..1476)
                     /locus_tag="PLR93_02960"
     CDS             complement(985..1476)
                     /locus_tag="PLR93_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25948.1"
                     /translation="MEQKTENKSVNRIGLLFLILGGVFAFISCLILVAGITLDLFGTE
                     TRGKMTNISYDAKRSDNPFTAQVTFTTANGEEKSFISWEDRFYFELDERIQRSGSTAP
                     FEGVKVRYLEAFPALAKVVFVYHAEYVNQIVWLFWSGVALMIGIISRRNKPITIDLSQ
                     RSK"
     gene            complement(1534..1668)
                     /locus_tag="PLR93_02965"
     CDS             complement(1534..1668)
                     /locus_tag="PLR93_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25949.1"
                     /translation="MSKQYKKKRSTTPAWVDSLIVILEILGAIGTVIAGIYSLLTWVF
                     "
     gene            1730..2371
                     /locus_tag="PLR93_02970"
     CDS             1730..2371
                     /locus_tag="PLR93_02970"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNH25950.1"
                     /translation="MHIPKYYREEDQDKIVEFLKANSFPAIVSSDGEKLLATHLPVEV
                     IENADGSLTVLGHMSRANPQWKSFGEQEVLLIFQGAHTYISPRWYNHVNVPTWNYINV
                     HVYGKVRMLEGEELHSLLSKLVSKHETGTGYSMEGLPADFVEKEMRGVAGFALDVTRL
                     DAASKLSQNRDDESYANIISHLEARGDDISAEIAHEMRDRRPSLTNPPQTDKI"
     gene            2423..2941
                     /locus_tag="PLR93_02975"
     CDS             2423..2941
                     /locus_tag="PLR93_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25951.1"
                     /translation="MTETNPSSEQNPQVTNLSQVDVETVNAQLVRMHQSSAGLVTSED
                     VDMTMSAVAQAKVTNLKLTDSGIAALHADTANVQNSVVGVAQTTELTLNGVSGAVVAG
                     SVEFGNAYAGVVAGREVRAERIESLILLSPNVQGNVTTVMDTRGAIIAGLVSGLFAGI
                     MLLLGRMLFGKK"
     gene            3045..3689
                     /locus_tag="PLR93_02980"
     CDS             3045..3689
                     /locus_tag="PLR93_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNH25952.1"
                     /translation="MEKVVLKATKRDVIGKQVKAMRREGKLPAVIYGRHLEPISIVLD
                     AHTAGLALSKVSASSLVTIDVEGKEYPALVRERQRDFIKGVLTHVDFLAVDMNEKIRT
                     SVGLAFVGVSGAVKDYNGILVHNLERLEVECLPGDLPERINVDISLLKQIGDIFRVKD
                     LAISEKVRILADEEEPIAVVTITKDEASAQSAAAGTGEATSPELSVERGKKDEE"
     gene            complement(3755..4570)
                     /locus_tag="PLR93_02985"
     CDS             complement(3755..4570)
                     /locus_tag="PLR93_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH25953.1"
                     /translation="MKVQNKVMVVTGGGNGMGRELVLNLLAKGARVAAIDINETALKE
                     TAQLAKEYKENIATFVLNITDKDAVDALPAKVIAKFGKVDGIINNAGIIQPFVRLVDL
                     DYSAIQRVLDVNLYGTIYMVKAFLPHLKERPEAHITNVSSMGGFLPVPGQTVYGASKA
                     AVKLLTEGLYAELLNTNVKVTLVYPGAIGTNIAANSGITVPNMPEADSKEASNFKQLA
                     PDKAAEIIVNAIENNRYSVLVGSDAKFMDFIYRLNPQNAAKFIYNQMKALLPS"
     gene            complement(4792..5391)
                     /locus_tag="PLR93_02990"
     CDS             complement(4792..5391)
                     /locus_tag="PLR93_02990"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNH25954.1"
                     /translation="MTEITEEYIWNKLNTTDRSRVETDGYAEIPIHPDVQLKCAAVLV
                     PLVFIQNEWHVLYTRRTDRVESHKGQVSFPGGACDEGETTPEETALREADEEIGLNPK
                     DVKVIGKLSRMVTISKFKVTPVVGIIPFPYAFKTSGAEVARVFTMPLLWLANRNNYWE
                     FSMPGSGRTLLAYHPYDGELLWGATARMTVNFLSLLDLL"
     gene            complement(5393..>5893)
                     /locus_tag="PLR93_02995"
     CDS             complement(5393..>5893)
                     /locus_tag="PLR93_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25955.1"
                     /translation="PAVPPALRNAHRLMESGNYAEAALAFYDLARKAEERFPERAPIL
                     YTEAGRAAVLNGDAKKGVTHFRSALTLLSTQQRYRRLQNLGERIVAELRERGLNAEAD
                     EVASVLRNNAQVSAGKEAPAPQKRAVLPTHCPSCGAAVRPDEVEWLNDVTAECDYCGS
                     PVRAES"
BASE COUNT         1528 a         1410 c         1437 g         1518 t
ORIGIN      
        1 ccgatggcgt gatagcggct catatctcaa accgtcattt agacttgcaa ccggtctttt
       61 ggcaattggc tcaggaatat ggcttgaaga tggtccgcat tgctaatgaa gctggcgctg
      121 aagaggaatt cccttctgaa tggctgctct taacgtcaaa tgaagatatt ctgaatattc
      181 ctcaaattca tgagcgtgcg ctagggtatg aaggctattc cacgtccatt ccaatatgga
      241 ctgatgatta cagtaactta tttcagattt tgaggtaaca aaaaagcgga cacttcagtg
      301 tccgcttttt tgttaggctg cagtgacggc aactactaaa ttaatagagc gccacaccca
      361 tcacagccac aaaatgtgca gccgcagcga gtagaacgaa aatatgccaa acttcatgaa
      421 agccaaatac gcctggtttg aagttgaaga tcttggtgat atagacgata gcgcccaagg
      481 tgtagatcac gccgccgatg atgagccagg tcaacaccca ggcaggaagc gccgcaagca
      541 tttgtccagc cgcagaaacg ctgaaccagc ccatgatgac ataaatccct gcatttagcc
      601 agcgtggcgc gcggatataa aagaccttga cgacaatgcc gaccaccacc aatgaccaga
      661 taatggagag catgccccac ttccagaatc cttcaaacgc gttaatgcaa aaaggggtat
      721 aggtccctgc gattagggca tagattgcag aatggtcgat cttgcggaag atttccaacg
      781 ccttatcttt gacctgtacc atatggtacg tggcactggc agagaacatg aagatcaggc
      841 tgatgccata gacgaccaat gagatgacct tggcaggggt actccaccca acgatgagca
      901 atgcgatcaa gccgatcaag gcaaggaccg caccgcccca gtgggtgagg ctattgacag
      961 gttcacgtaa ttttttgagc atagttattt actcctttga ctgagatcaa ttgtaattgg
     1021 cttattacga cgggaaatga tcccgatcat caacgcaaca ccagaccaga aaagccaaac
     1081 gatctggttc acatattcgg catggtacac aaagactacc tttgctagag cagggaaggc
     1141 ttcaagataa cgaactttta cgccctcaaa tggtgccgta ctaccgcttc tctgaatgcg
     1201 ctcatccagc tcaaaataga aacggtcttc ccaagagata aaagattttt cctcgccgtt
     1261 tgctgtcgtg aatgtgactt gcgctgtaaa ggggttatca gaccttttgg catcgtatga
     1321 tatgttggtc attttccctc tggtttcggt tccgaaaaga tcaagagtaa ttccagcaac
     1381 caatatgagg caactaataa aagcaaaaac acctccgaga attaggaata gtaaaccaat
     1441 tcggttaacg cttttatttt ctgtcttttg ttccacgagg gtctcctacc tctaaaattt
     1501 tatgaccaag gttcagatta taaccaaggt ttatcaaaac acccacgtaa gcaggctata
     1561 aatgcctgcg atcacagtgc caatagctcc aaggatttct aaaataacaa tgaggctgtc
     1621 tacccaagct ggagtagtag accgtttctt tttatactgt ttcgacatat cgcaaaattc
     1681 tacctgagcc tcttaagaaa tattgttata ctttccttag aggtttaaca tgcatatccc
     1741 aaaatattat cgtgaagaag accaagataa gattgtggaa tttctaaaag ccaatagttt
     1801 tccggcgatt gtctcgagcg atggtgaaaa attacttgcc acgcatttac cggtggaagt
     1861 tatagaaaat gccgatgggt ctctgaccgt tctggggcat atgtctcgcg ccaacccgca
     1921 gtggaagtct tttggcgagc aggaagtttt gcttattttt cagggtgcgc acacttatat
     1981 ttctccgcgc tggtataacc atgtcaatgt gccaacctgg aattacatca atgtgcatgt
     2041 gtatggaaag gtccgcatgt tggaagggga ggaattacat tccctgctta gcaagttggt
     2101 ctctaagcac gaaaccggca caggctatag catggaaggc ttgcccgccg acttcgttga
     2161 aaaagagatg cgtggggtgg caggttttgc tttggatgta acccgcctcg acgccgcctc
     2221 gaaactgagc caaaaccgcg acgatgagtc ctatgcaaac attatcagcc acctcgaagc
     2281 ccgcggagat gatatttcag ccgagatcgc gcacgaaatg cgggataggc gcccttcctt
     2341 gacgaacccc cctcaaacgg ataaaatctg agccgttatt caacctgcgc ctccgtgagg
     2401 aggcgtattt tctgccaaaa tcatgaccga gacaaatcct tcttctgagc aaaacccgca
     2461 ggtgaccaac ctctcccaag tggacgtgga aacggtcaac gcgcagttgg ttcgcatgca
     2521 ccaaagctcg gctggtttgg tcacttcaga agatgttgat atgaccatga gtgcggtagc
     2581 gcaggcgaag gtcaccaact taaagttgac cgactctggc atagcggctc tgcatgccga
     2641 cactgctaat gttcaaaatt cagtagtagg agtcgctcaa acgactgaat taaccttgaa
     2701 tggcgtatca ggcgcggtcg tggcaggaag cgtcgagttt ggcaacgcct atgctggcgt
     2761 tgtggcagga cgtgaagtcc gtgctgagcg cattgagtcg ctaatcctat tatcgcccaa
     2821 tgtgcagggg aatgtcacta ccgtaatgga tactcgcggt gcgattattg ccggcttggt
     2881 aagcggtttg tttgcaggca ttatgctttt gctcgggcgc atgttgttcg gcaaaaaata
     2941 attcggatgt tcagcgggaa tgacccgctt tacagaaatt cattttgggg cgaaggtaac
     3001 acagcggagt tgctgtgtga gaggcgccca aggagttagg taagatggaa aaagtagtat
     3061 tgaaagcaac caaacgtgat gtgatcggca agcaggtgaa agccatgcgc cgcgaaggga
     3121 aactccctgc ggtgatttat ggtcgtcatc tcgaaccgat ttccattgtt ttggatgcac
     3181 acaccgccgg cttggctttg tcgaaggttt ctgcttccag cctcgtgacg attgatgtgg
     3241 aaggcaagga ataccctgct ctcgtccgcg aacgccagcg tgactttatc aaaggtgttc
     3301 tcacccatgt agacttcctc gccgtggaca tgaacgaaaa gatccgcacc agcgtgggac
     3361 tcgctttcgt gggtgtttct ggcgccgtga aagattacaa cggtatcctc gtccacaact
     3421 tggaacgcct cgaagtggaa tgtttaccag gtgatcttcc tgaacgcatc aatgtagata
     3481 tctctctgct caagcagatc ggcgatatct tccgcgtgaa agacctcgcg atctccgaaa
     3541 aagttcgcat cttggcggat gaagaagaac ccattgccgt ggtgaccatc accaaggatg
     3601 aggcttctgc ccagtctgct gctgccggca ccggcgaagc gacgtctccc gagctcagcg
     3661 tggaacgtgg caagaaagac gaagaataat ttcttctgta ctagcaataa aaaagtccgc
     3721 catcgcaaga tggcggactt tttttaccaa tgtattacga aggtagcaag gctttcattt
     3781 ggttatagat aaacttggct gcattttgcg gattcagtct gtagatgaaa tccatgaact
     3841 ttgcatctga gcctacgaga acgctgtagc gattattctc gatggcattc acgatgattt
     3901 ctgccgcttt gtctggcgct aactgcttaa agttactggc ttctttactg tccgcttctg
     3961 gcatgttcgg tactgtgatg cccgaattcg cagcgatgtt tgtaccaatg gcgcccgggt
     4021 acaccaaggt cacttttacg ttggtgttca gcaattctgc atataaacct tcggttagca
     4081 acttaactgc agccttgctc gcgccataaa ccgtttgccc agggaccggc aagaacccgc
     4141 ccatgctgga aacgttggtg atgtgagctt cagggcgctc cttcaaatgc ggcagaaaag
     4201 ctttgaccat gtagatggtg ccgtacaggt ttacatccaa tacacgttga atggcggaat
     4261 agtctaagtc aacgagtcgc acgaaaggct gaataatgcc cgcgttgttg atgatgccat
     4321 ctacttttcc gaatttggca atcactttgg caggcaacgc atcgacagcg tctttatctg
     4381 taatgttcaa gacgaatgtc gctatatttt ctttgtactc tttcgccaat tgagcggttt
     4441 ctttcaaagc agtttcgttg atatcgatgg cagccacacg tgcgcctttt gctaaaaggt
     4501 tcaacaccaa ctcacgtccc ataccgtttc cgccgcccgt tactaccata actttatttt
     4561 gtactttcat gttgatctct cctttatcaa tccttgggtt gctttcatct ccataagggg
     4621 aggggcaccg agattgttgt ttgatcgttc tgatcgtctg tattgtaggt ctgagggaga
     4681 gattttatta gtgaaattaa tcacaaataa agaggattat tgtgatattt ttcacaatat
     4741 agagggtgga aattgtccta aatctaactt caagttatat atctggagaa actacagcaa
     4801 atctaagaga gacaagaagt tgacagtcat gcgtgctgtg gcgccccaga gaagttcacc
     4861 gtcatatggg tgataggcga gcaaagtccg cccagagccg ggcatggaaa attcccaata
     4921 gttattgcgg ttggcgagcc aaagcaaagg catggtaaag acccgcgcca cttctgcacc
     4981 agaggtctta aacgcataag gaaaaggaat gatcccaacc acaggggtca ctttgaattt
     5041 actgattgtc accatgcggc tgagtttgcc aatcactttt acatctttag gattcaaccc
     5101 gatctcttca tcggcttcgc gtagagcggt ctcttcgggc gtggtttcgc cttcatcgca
     5161 agcaccgccg ggaaaagaga cttgcccttt gtgcgattca acccggtctg tgcggcgggt
     5221 atatagcaca tgccattcat tttggatgaa taccaacgga accagaaccg ccgcgcattt
     5281 caactgcaca tctggatgaa tcggaatctc ggcatacccg tccgtttcca cgcgcgaacg
     5341 gtcggtcgta ttgagcttat tccaaatgta ttcttctgta atctcggtca tactaagatt
     5401 cagcgcgcac cgggctgccg cagtaatcac attctgcggt cacatcattc agccattcga
     5461 cttcatccgg gcggactgcc gcaccacacg atggacaatg ggttggcaag accgctcgct
     5521 tttgcggtgc tggcgcttct ttccccgccg aaacttgggc attgttcctg agaacggatg
     5581 ctacttcgtc agcttcggca ttcaagccgc gttcacgtaa ttccgctaca atgcgctcgc
     5641 caaggttctg caaacggcga taacgttgtt gtgtgcttag taaggtcagc gcactacgaa
     5701 aatgggtaac gccttttttt gcgtcgccat tcaaaaccgc agcacgaccc gcttctgtat
     5761 aaaggatcgg tgctcgttca gggaagcgtt cttcagcttt tctggcgagg tcataaaaag
     5821 caagtgcagc ttctgcataa ttccctgatt ccattaagcg atgggcattt cgtaaggcag
     5881 gtggcaccgc cgg
//
LOCUS       DAOQFT010000073         5857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6850_length_5857_cov_5.752499, whole genome shotgun sequence.
ACCESSION   DAOQFT010000073 DAOQFT010000000
VERSION     DAOQFT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6850_length_5857_cov_5.752499"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1518
                     /locus_tag="PLR93_03000"
     CDS             157..1518
                     /locus_tag="PLR93_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH25956.1"
                     /translation="MAYDYEDALDYSSHWMEWIQKNEFPTPEQAHKIIEESTTNFAEY
                     YNRNWLEYRKSVTESGDWAAVEWGGSGAVFKDVLGREYLDFLGGYGMMDLGWSHPEVV
                     NTVKAQLERSPMPSQELLDPLRGVLGRLLASITPGDLKYSWFGASGTEANEAAMKIAK
                     LYTGKTAFIVGVKAFHGKTMGSLSLMGKSDYRAPMGAMYSGQVYHVPFGDADAVEKQL
                     DICEKVGIGVAAVMFEPIQGESGAIVPPDDFWPRIRAATKKHGVLLIADEVQTGLGRT
                     GKLWGVEHWNVVPDILTVAKSLGGGVMPISAVTTTEEIFHPMMYPNPFMHTTTTGGGA
                     LACSAAISAIHVTLREKLWEQAAEKGAYLMPKLENFVVQYPSIFEKVTGKGLLIGMHF
                     KNPEIGYKVASGLFKRGVLVAGTLTSAQTVRIEPPLVVTYPQMDALLDRLDEVLKEIN
                     KSM"
     gene            1591..2706
                     /locus_tag="PLR93_03005"
     CDS             1591..2706
                     /locus_tag="PLR93_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH25957.1"
                     /translation="MTSEFAVEMRDVVKKFITPEGNELNAVDHVTMQIKNGEFFSMLG
                     SSGCGKTTSLRMIAGFEWPTEGEVYIEGKPMGHTPPFQRKVNTVFQSYALFQHMTVFQ
                     NIAFGLEMEGAKEEEIKKRVGRALEMVQLTGMERRRPKQLSGGQQQRVAVARALVKTP
                     DVLLLDEPLGALDLKLRKEMQLELKALQQQLGITFIYVTHDQEEALTMSDRIAVMSKG
                     RVQQLATPVEIYERPVNKFVADFIGESNFLDGKIKSISKDEAVVFVPALNTELKGMPM
                     SKDFVNGEEVAVSVRPEKIRIVENESKGDGLFKAKVLTSVYIGSDTHVYVDMEGIRLK
                     VMEQNRISRLDPSSFYSKDQVVTLLFMPENTLVLKKD"
     gene            2709..3632
                     /locus_tag="PLR93_03010"
     CDS             2709..3632
                     /locus_tag="PLR93_03010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25958.1"
                     /translation="MLQRLRENKSMQGTFLALPTTLWLFVLLIIPLFLTLVVSFGQRS
                     PDGDVIYSFGLHNYIRLIGYSTDCPDGQSACFDPLYVNILWRSLTLAFNTTAIVIMLA
                     YPLSYFIARSHPNRRNMFLFMVLIPLWTNFVIRVYAWMMLLRKEGAINIIIGWVANLL
                     HIPFTPLEMLYTPGAVLVGMVYEFLPFMILPIYTSLEKIDNSLYEAAADLGANAFRTF
                     MRVTLPLSMPGVVAGTILVFIPVMGTFIVSDILGGRQVILVGNLIQRQFLDARDPTFG
                     SAASLILMVLTLIVTYFYTRKFGFGEEIIAA"
     gene            3673..4572
                     /locus_tag="PLR93_03015"
     CDS             3673..4572
                     /locus_tag="PLR93_03015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25959.1"
                     /translation="MNPYRRSPGVIAATVFVYFFLYAPIVMLILYSFNSSRANVKFEG
                     FVTSFSDRVKMDGSTVVSSPCGPFHWYCDLAKNDDVIEAAGNTLSIAFTATIVSTIIG
                     TMAALALQRYDFKLKPFSQLALYIPIVIPEIVMGIGILTLFSQAFGWLNNALNLTGDA
                     RLALGIGTVTVSHIAFMVPFVTLVVQARLQGFDKSYEEAAMDLGANEWITFRRVTFPM
                     ILPGVLSGALLAFTLSLDDFVITFFTNGPGSTTLPIYVYGLLRRIITPQVNALSTVWI
                     LTVFIAVVVLQWIQSREQKPSHS"
     gene            4628..5788
                     /locus_tag="PLR93_03020"
     CDS             4628..5788
                     /locus_tag="PLR93_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNH25960.1"
                     /translation="MLRKSLFNILAVLAILSFALTACGGGGGDAEAPAAGDGPQVTSS
                     GFECPEPNPRLDVTSTELNIFVWTEYFPQDMLDCFELVYGITLNRDEYSSNEEMYAKV
                     AAGGTAYDLAQPTDYIVPLMIRQELVQELDHSKLPLLSNFDPNWMDQPFDPGNKYSLP
                     YLAGTDAIVVNTATVENVPTSWADLWNPEYAGRMVFLDDSRAVIGLTLVTLGYDPNTT
                     DPAQLEEAKNKLAELVPNIKLFDSDSPKTALIAGDVDLGMTWTGEAFIANQENPDIQY
                     VYPEEGPVLWQDNWVMLKDASHADAAYAWMSYIMQGDVFWLTIRDFQYTNPNAAAIEY
                     AKNNQPEVYDAYTNSPITNPPAEAIAKGFGIEDVGDATPLYDNIWVEIKGGN"
BASE COUNT         1408 a         1373 c         1494 g         1582 t
ORIGIN      
        1 agcggttgga aagattcatt ctttggcgac atgcacgggc agggcatgga tgcagtggag
       61 ttcttcacac aaaagaagat tgtagtggaa cgctggccca aagagtggac gagaaagttc
      121 taaatctata aatcaaggat acggtaacaa acaatcatgg catacgatta cgaagacgct
      181 ttggattatt catctcactg gatggaatgg attcagaaaa atgaattccc aaccccggag
      241 caggctcaca agattattga agagtctaca accaattttg ccgagtatta caaccgcaat
      301 tggcttgagt accgtaaatc ggtgactgag tcgggcgatt gggcggcagt agaatggggc
      361 ggttcgggcg cggtattcaa agatgtactt gggcgtgaat atcttgattt ccttggcggc
      421 tatggcatga tggatttggg atggagtcat cctgaggtgg tgaacacggt caaggcgcag
      481 ttggagcgtt cgcccatgcc ttcgcaggaa ttgcttgacc ccttgcgggg tgtcttgggg
      541 cgtttgttgg cttcgatcac accgggcgat ctaaaataca gttggtttgg tgccagcggc
      601 accgaagcca atgaagcggc gatgaagatc gcaaagctgt atacaggcaa aacggcattc
      661 atcgtaggtg tgaaagcctt ccacggtaag accatgggtt cgctttcatt gatgggcaag
      721 tcagactatc gtgcgcctat gggcgccatg tatagcggac aggtgtatca cgttccgttc
      781 ggggatgcag atgccgttga aaagcaattg gatatctgtg agaaggttgg cattggcgtc
      841 gcggcagtga tgtttgagcc gattcaaggc gagtcgggtg ccatcgttcc gcccgatgat
      901 ttctggcccc gcatccgtgc tgcgacgaag aagcatggcg tcttgttgat cgcggatgaa
      961 gtgcaaacgg gtctgggtcg gaccgggaaa ctgtggggcg ttgagcattg gaatgtcgtc
     1021 cctgatattt tgaccgtggc aaagtcattg ggtggtgggg ttatgcctat cagcgctgtg
     1081 actactacgg aagaaatctt ccacccgatg atgtatccga atccgttcat gcatactacg
     1141 acaacgggcg gcggtgccct cgcatgttcg gcagcgatct cagctatcca tgtcactctg
     1201 cgcgaaaagc tttgggagca ggcagctgaa aaaggtgcct acctgatgcc aaaattggaa
     1261 aattttgttg tacaataccc aagcattttt gaaaaagtga caggcaaagg cttgttgatt
     1321 ggtatgcact tcaaaaatcc tgaaatagga tataaagtgg cttctggtct tttcaagcgt
     1381 ggtgtcttgg ttgccggaac gctgaccagc gctcaaaccg ttcgcattga accacctttg
     1441 gtcgtaacct acccacaaat ggatgctttg ttagatcgct tggatgaagt gctgaaagaa
     1501 atcaataaat ctatgtaatt catacttccg aaagatcggg aaacactccc cgaatttcgg
     1561 aagtctatta tttcatgacg gagaagatag atgaccagtg aatttgctgt cgagatgcgt
     1621 gatgttgtaa agaagttcat aacgccagaa gggaatgaat tgaacgccgt cgatcatgtc
     1681 acgatgcaga tcaagaacgg tgaattcttc tcaatgcttg gttcctcagg ctgtggcaaa
     1741 actacgtccc tgcgtatgat cgcaggcttt gaatggccca cagaagggga ggtctatatt
     1801 gaaggcaagc ccatggggca tacgccacct ttccaacgca aagtgaatac tgtctttcaa
     1861 agttatgcct tgttccagca tatgacggtg tttcagaata ttgcttttgg tctcgagatg
     1921 gaaggcgcca aagaggaaga gatcaagaaa cgcgtcggac gtgcattgga gatggttcag
     1981 ctcacaggta tggaacgccg ccgccccaag cagctttctg gtggtcagca acaacgtgta
     2041 gcagttgcgc gtgctttggt taagacgcca gatgtgcttc tgctcgatga accgctcggc
     2101 gcattggact taaaactccg caaagaaatg cagcttgaac ttaaagcttt gcaacagcaa
     2161 cttggcatca cattcattta cgttacacac gaccaagaag aagctctgac catgtctgac
     2221 cgtattgcag tcatgagcaa aggacgtgtt cagcagcttg ccacaccagt tgaaatttac
     2281 gaacgcccgg tcaataaatt cgtggcagac tttatcggcg agtcaaactt cctagatggc
     2341 aagatcaagt ccatctcgaa agatgaggcg gtggtttttg tcccggcgct caatacggag
     2401 ttgaagggca tgcccatgtc gaaggacttc gtcaatggag aggaagttgc tgtttcggtt
     2461 cgcccagaaa agattcgtat cgtagagaat gaatccaagg gtgatggtct cttcaaggca
     2521 aaggtactta cttctgtgta cattggctct gatacccatg tgtatgtgga catggaaggc
     2581 attcgtttga aggtgatgga acaaaaccgc atttcacgcc ttgacccatc ctcgttctat
     2641 tcgaaggatc aagtggttac gttattgttc atgcctgaga atacgcttgt cttgaaaaag
     2701 gattagccat gttacagcgc ttacgtgaaa acaaatcgat gcagggaacc ttccttgcgc
     2761 ttcccaccac tttgtggctg ttcgtgcttc tgatcattcc tttgtttctc acattggtgg
     2821 tcagcttcgg tcaacgcagc ccagatggcg atgttatcta ttcctttgga ctccataact
     2881 atattcgttt gatcggttac agcaccgact gtccagatgg gcagtctgct tgttttgacc
     2941 cactgtacgt caacattttg tggcgttcat tgacgttggc tttcaacacc actgccatcg
     3001 taattatgct ggcgtatccg ctttcgtatt tcattgctcg ttcacacccg aaccgcagga
     3061 atatgtttct ttttatggtg ttgattcctc tatggacgaa cttcgtgatc cgcgtttatg
     3121 cttggatgat gttgcttcgc aaggaaggcg ctattaacat cattatcgga tgggttgcga
     3181 acttgctgca cattccattc acacctttgg aaatgttata cacgccgggc gcggtcttgg
     3241 taggcatggt gtatgagttt ttgccgttca tgatcctgcc gatctacacc tcgcttgaga
     3301 agatcgataa ctcattgtat gaagctgccg cagaccttgg cgccaatgcc ttccgaactt
     3361 tcatgcgtgt taccttgccg ctttccatgc cgggcgtggt ggctggaact atcttggtat
     3421 tcatcccggt gatggggaca ttcattgtgt ctgatatctt gggcggacgc caagttattc
     3481 tggtggggaa cctgattcag cgtcaattct tggatgcgcg tgacccaacc ttcggttcgg
     3541 cggcttcgct tattttgatg gtgctaaccc tgatcgttac atacttctac acccgtaaat
     3601 ttggatttgg agaggagatc attgcagcat gatcccacac actacaaacc tttttattaa
     3661 ggaggaaatg cgatgaatcc gtatcgccgt tcccctgggg taatcgccgc cacggtcttt
     3721 gtgtatttct ttctgtacgc accgattgtg atgctgatct tatattcctt taactcttca
     3781 cgcgcgaatg tgaaattcga aggctttgtc acatccttca gcgaccgagt gaaaatggat
     3841 ggcagtactg tcgtgtctag cccatgtgga ccctttcact ggtattgtga ccttgcaaaa
     3901 aatgatgatg tgatcgaagc cgcaggcaat acgttgagca tcgcatttac cgctacgatt
     3961 gtttctacca tcattggtac gatggcggct cttgcgttgc aacgttacga tttcaaactc
     4021 aaaccctttt cgcaactggc tttatatatc cctatcgtga tccctgagat cgttatgggg
     4081 attggtattc tgacactgtt tagtcaggct tttggatggc taaacaatgc tctcaatttg
     4141 acaggtgacg ctcgcctggc attgggcatt ggcacggtga ctgttagtca tatcgcgttc
     4201 atggtgccgt ttgtaacgct cgttgtgcaa gctcgcttgc aaggctttga caagtcttac
     4261 gaagaagccg ccatggattt gggcgctaac gaatggatca cattccggcg tgtaaccttc
     4321 ccaatgattt taccgggtgt gctctcgggt gctttgcttg ccttcaccct ttctttggat
     4381 gactttgtaa tcaccttctt cacgaatgga cctggctcta cgaccttgcc gatctacgta
     4441 tatggtctgt tacggcgcat tattacgccg caagtgaatg cgctttcaac agtttggatt
     4501 ttgaccgttt ttattgcggt tgtagtcttg caatggattc aaagccgcga gcagaaaccc
     4561 tctcattcat aatgggtata tcgtgcgtga gacccgcacg ttgctaacta tataaaggag
     4621 aagaaacatg ttacgaaaat cgctattcaa tattcttgcc gttctggcga tcctcagctt
     4681 tgcgctgacc gcctgtggtg gaggcggtgg agatgcggaa gcgcccgccg ccggggatgg
     4741 tcctcaagtg acctcttctg ggtttgaatg cccggaacca aatccgcgtt tggacgttac
     4801 ctccaccgag ttgaatatct tcgtgtggac ggagtacttc ccacaagata tgttggactg
     4861 ttttgaactg gtatatggca tcacattgaa ccgcgacgaa tactcctcca atgaggaaat
     4921 gtacgcgaag gttgcagctg gtggtaccgc ctacgatctg gctcagccaa ccgattacat
     4981 cgtgccgctc atgatccgtc aggaattggt tcaggaactg gaccactcca aacttccact
     5041 tcttagcaac tttgacccga actggatgga tcagcccttc gaccccggta acaagtactc
     5101 actgccatat cttgctggca cagacgccat tgttgtgaac actgcaactg tcgaaaatgt
     5161 accgacctcc tgggcagacc tgtggaatcc agagtatgcc ggacgcatgg tcttcttgga
     5221 tgactctcgt gctgtgatcg gtctcacctt ggtaaccctt ggctatgacc ccaacacaac
     5281 cgaccccgct caattggaag aagccaagaa taagttggca gaacttgttc cgaatatcaa
     5341 gctcttcgat agcgatagcc ccaaaaccgc cctcatcgct ggcgatgttg acctcggcat
     5401 gacctggact ggtgaagcct ttattgccaa ccaggaaaat cctgatattc aatacgtcta
     5461 ccctgaagaa ggtcctgttc tttggcagga taactgggtc atgttgaagg acgcttctca
     5521 tgccgatgcc gcctatgcat ggatgagcta catcatgcaa ggcgatgtgt tctggctcac
     5581 gattcgtgac ttccagtaca ccaacccgaa tgctgctgcc attgaatatg caaaaaacaa
     5641 tcaacccgaa gtctatgatg catataccaa ctccccgatc accaacccgc ctgctgaagc
     5701 cattgccaag ggctttggta tcgaagacgt gggcgacgcc accccgttgt acgataatat
     5761 ctgggtagag atcaaaggcg gcaattagcc tttcttcctg aaaattcaaa aaacgccgtc
     5821 aacatactgt tgacggcgtt ttttgctgcc tagaaat
//
LOCUS       DAOQFT010000074         5849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6867_length_5849_cov_6.983259, whole genome shotgun sequence.
ACCESSION   DAOQFT010000074 DAOQFT010000000
VERSION     DAOQFT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6867_length_5849_cov_6.983259"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PLR93_03025"
     CDS             <1..1432
                     /locus_tag="PLR93_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNH25961.1"
                     /translation="PAPTDIHMWHNYINATSIDEVVRILAEKRESARVVAGGTDLVLE
                     LERGVRKGVDTLIDVTRIPQLDQITIDEDDVIHLGALVTHNDCAASKLIRTRACPLAR
                     ASWEVGAPQIRNRGTVAGNLITGSPANDTITPLMALGAKVTLVSDRKTRVVPLKKFYL
                     GVRKTVMEPDEMLVDISFPALTKTQRGTFIKLALRRAQAISLVNAAIILDLKADTVKS
                     ASITLGAVTPIITHAPKAEKFLIGKKLNDKNIAAAAELAMKSAKPIDDVRGSANYRRE
                     MARVITQRGLTSIRNGKEQTGMPKKPILLVSEKTTQHGSNIAGSFPENKIETIINGKK
                     YTFTSGHDKTLLRLLREEGMLIGTKEGCAEGECGACTIYLDGQAVMSCLVPAPRAHGA
                     EITTIEGLANGEKLNPVQEKFIEHNAVQCGYCIPGFIMSGAKLLEEKPNPTRNEIEQA
                     ITGNLCRCTGYYKIVKAIEEASNSSS"
     gene            1529..1864
                     /locus_tag="PLR93_03030"
     CDS             1529..1864
                     /locus_tag="PLR93_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25962.1"
                     /translation="MMIVLPAISMAAGYELVNLALASGYPVPYQLLGTPRFPDLFYKS
                     SGMMTILSPIIAIRNFYAYAVAAIFIMVLLSGIMSLVYAIVYRMIGPSRYSPLDAPPA
                     NVKAKAYKR"
     gene            1866..4055
                     /locus_tag="PLR93_03035"
     CDS             1866..4055
                     /locus_tag="PLR93_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNH25963.1"
                     /translation="MTKKIVGTSIPRIDALGKVIGETEYSGDRHLPGMLYMKTLMAER
                     PHARVREIRIDKALAAPGVVAIYTAKDVPINEYGLQIPDQPVLCGPGSSKPYTDIVRF
                     VGDQVAAIVAETESQAQAAMKLIEVDYEDRPPLTDPVIAMQPDAPLLHPDRGDTNVCV
                     HYRIRKGNVDDAFAKADVIVEGEYQTPVQEHAYLQPEAGLAYIDEDGLIVVECAGQWT
                     HADRKSIAHALDVPEEKVRVIYPAIGGAFGGREDLSVQIVLALAAMKLNRPIKIIWSR
                     RESMIGHGKRHAVLLRAKWGATKDGKVVAVQNEIIGDGGAYMYTTNKVLGNATITCTG
                     PYSVDNVKTDVYGVYTNNVPGAAFRGFGAPQALFMAESQMNKLAEKLGMDPVEFRLKN
                     ALRDGDTLDVGTPAPTPVNIVECIEAARDKYQWKKQGKSKTKKQGAIIKGRGFATGFK
                     NVGFSFGYKENCWAKVEIHGNGSIERVVLHHAGAEVGQGFHTVMTQMAAHVIGIPTKL
                     VEFRASDSATQGNPGSASASRLTFMAGNSVKGAAEAALAKWEAEERPAIAEFSYFAPP
                     TTPMDKETGYSMPNFQYAYVAQAVEVEVDTETGHVRVKRIISADDVGTAINPDLVVAQ
                     IEGAVVQAHGYAMMEEFKTKNGRVLTDQFSTYLLPTIWDIPEKVESVLVEIPDPNGPW
                     GARGVGELPYLPVAAAIAAAIHDATGVWISEFPFTPERVLRALGKIK"
     gene            4103..4894
                     /locus_tag="PLR93_03040"
     CDS             4103..4894
                     /locus_tag="PLR93_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNH25964.1"
                     /translation="MNSIFQALAELEKNKQPAALCTVVKSEGSTPRHVGSKMLVYPDG
                     TFIGTVGGGDLEHRVLDEAWMALSDGQSRHLHYTMSDPSRGDPGVCGGQVEVFVEPIL
                     PPATLVVIGAGHVGKAVVHLAKWLGFHVVVSDDRAEFCTPEATPGADAYYSVEMGKLA
                     ETLKITNRTYLVVTSRGSSVDALGLPSLLESEAAYIGVIGSKRRWATTVKALKENGVK
                     EELIAKVHSPMGLELQAETPEEIAVSIMAEVLLVRDKGTGKSMRA"
     gene            4896..5489
                     /locus_tag="PLR93_03045"
     CDS             4896..5489
                     /locus_tag="PLR93_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH25965.1"
                     /translation="MTLPSNQRMFHGGIRAAALADQLGARFNSRQRRVSVHSQGDTAL
                     VQIGSKHGTPLTVHIADTEGGVLVTMSRERNWLDKAADTSEMMERAANSPLSLLAMIP
                     DIVGEMSQENIAPKVWDAINDLCSLTRSLAGEKNAPRNPKVCTFCGTANDPELELCVA
                     CGGSLPTDLPRVCPKCSRGHTSDALFCQACGTRLVEG"
     gene            5605..>5849
                     /locus_tag="PLR93_03050"
     CDS             5605..>5849
                     /locus_tag="PLR93_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH25966.1"
                     /translation="MARIFDVIEYANEMNDEIVHRFPEEGIGDFRIGSQVIVRESQNA
                     VFFRDGNALDVFKAGRHTIATANIPMLIDFIGKAFNDR"
BASE COUNT         1603 a         1448 c         1438 g         1360 t
ORIGIN      
        1 ccccgcccct acggatattc atatgtggca caactatata aacgcaacat ccattgacga
       61 agtcgttcgt atccttgctg agaagcgtga aagtgcccgc gtcgtcgcag gtggaacaga
      121 ccttgttctt gaacttgaac gtggcgttcg caaaggcgtt gatacactca tcgacgttac
      181 tcgcattccc caactagatc aaatcacaat tgatgaagat gatgttattc atcttggtgc
      241 actggtcacg cataatgact gtgcggcatc caaactcatc cgcaccagag cctgtccgct
      301 ggcacgtgcg tcttgggagg tcggcgctcc gcaaattcgt aatcgcggaa ccgtagcagg
      361 caacctcatc accggctctc ccgcaaacga taccattact cctctcatgg cgcttggtgc
      421 caaagttaca ttggtctcag atcgaaaaac ccgagttgtt ccactcaaga agttttatct
      481 cggtgtacgc aaaaccgtta tggaacctga tgaaatgctg gtggatattt ccttcccggc
      541 attgaccaaa actcagcgtg ggacgttcat caaattggca ctgcgccgcg ctcaagccat
      601 ctccctcgtc aacgccgcga tcattctgga cctcaaagcg gataccgtta aatctgcctc
      661 gatcacactg ggagccgtta cacccatcat tacccatgca ccaaaagctg aaaaattcct
      721 cattgggaaa aaactcaacg acaaaaatat agctgccgct gcagaactag cgatgaagtc
      781 tgccaagccg attgatgacg tgcgcggctc tgctaactat cgccgcgaaa tggcacgtgt
      841 catcacccaa cgtgggctga catccattcg caacggcaaa gaacaaacag gtatgcccaa
      901 aaagccaatc ttactggtga gcgaaaaaac aactcaacat ggctctaaca ttgcaggttc
      961 tttccctgaa aataagatcg aaacgattat caatggcaag aaatacacgt tcacaagcgg
     1021 gcacgacaag accctgttac gtttgctacg cgaagaagga atgttgatcg gcaccaaaga
     1081 aggctgcgcc gagggcgaat gcggggcatg tactatctat ttagacgggc aagccgtcat
     1141 gtcttgcctt gtccctgccc cacgggcaca tggcgcagaa attaccacca tcgaaggttt
     1201 ggcaaatgga gagaaactaa atccggttca agaaaagttt attgagcaca atgccgttca
     1261 atgcgggtac tgcatccccg gctttatcat gtctggtgcg aaacttcttg aagaaaaacc
     1321 aaacccaact cgcaacgaga tcgaacaagc cattaccggc aatctctgcc gttgtaccgg
     1381 atattacaaa atagtaaaag ccattgaaga agcaagtaat tcatcgtctt aagaggtatg
     1441 aatgagtaaa tacggtggtt cgtccagaaa agtaataaaa gaaagaaaca aacagccaca
     1501 ttatctttgg cgaggtattg gctgtcttat gatgatcgtc ctccctgcca tttccatggc
     1561 agccgggtat gaactcgtca acctcgctct tgcaagcggg tatccagttc cgtatcaact
     1621 gcttggcact ccaagatttc cggacctttt ttacaaatcc agcggcatga tgaccattct
     1681 cagccccatc attgctattc ggaattttta tgcctacgct gtagccgcta ttttcattat
     1741 ggttctgcta agcggtatta tgtcgctcgt gtatgctatt gtgtaccgca tgattggtcc
     1801 atcacgctat agccctctcg atgctcctcc agcaaacgtc aaagcaaaag cctataaaag
     1861 ataaaatgac aaaaaaaatc gttggaacct ccatccctcg tattgatgca ctcggtaagg
     1921 tgatcggcga aactgaatac tccggtgacc gtcacctgcc gggtatgctt tacatgaaaa
     1981 ccctcatggc agagcgccct catgcccgcg tgcgggaaat tcgcattgat aaagcccttg
     2041 ctgcgccggg cgttgttgcc atatacacag caaaagatgt tccaatcaac gagtatgggc
     2101 tccaaatccc agaccagccc gtcctttgcg gacctggttc cagcaagccc tacacagata
     2161 tcgtccgctt tgtaggagat caagtcgcag ccatcgtagc agaaacagag tctcaagctc
     2221 aagcagccat gaaactcata gaggtggatt atgaagaccg accgcctctt acggacccgg
     2281 tcattgctat gcagcccgac gcccctctcc tccacccaga ccggggtgac accaatgtat
     2341 gtgtccatta ccgaatccgc aaagggaatg tggacgatgc ctttgcgaaa gccgatgtga
     2401 tcgttgaagg cgagtatcaa actccggtgc aagaacatgc ctatctccaa cctgaagcgg
     2461 gattggcata catagacgaa gatgggctca ttgtcgtcga gtgtgctggt cagtggacac
     2521 acgctgaccg taaatccatt gcacatgcat tggatgtgcc tgaggaaaaa gtccgcgtaa
     2581 tttaccctgc gatcggcggc gcatttggag ggcgtgaaga cctttcagtg caaatcgtgc
     2641 ttgcccttgc agccatgaag ctaaatcgcc caataaaaat catctggtca cgccgcgagt
     2701 cgatgatcgg gcatggaaag cgtcatgccg ttttgttgcg cgccaaatgg ggcgcaacaa
     2761 aagacggcaa agttgttgct gttcaaaatg aaattatcgg ggatggtggt gcatatatgt
     2821 acaccaccaa taaagtctta ggaaacgcca ccattacctg tacgggtcct tactctgttg
     2881 ataacgtaaa aaccgatgtt tacggtgtgt atacaaacaa cgtccctgga gcggcttttc
     2941 gcgggtttgg cgcgcctcaa gcgttattca tggcagagag ccaaatgaat aaacttgccg
     3001 agaaacttgg tatggacccg gttgagttcc gtttgaagaa tgcattacgc gatggcgata
     3061 cgctagatgt cggcactcca gcaccaaccc cggtaaatat tgtagagtgt atcgaagcgg
     3121 ctcgtgataa ataccaatgg aagaaacaag ggaaaagcaa aacaaagaag cagggcgcta
     3181 ttatcaaagg gcgcggtttt gcaaccggct tcaagaatgt aggtttctct tttggttata
     3241 aagagaattg ttgggcgaag gttgaaattc atggcaatgg aagcattgag cgtgttgtac
     3301 tacatcatgc tggtgccgaa gttggtcagg gtttccatac tgtcatgacc caaatggcag
     3361 cacacgtaat cggcatacca accaagcttg ttgaatttcg cgcctccgac tcagcgacac
     3421 aaggcaaccc cggctcggct tcagcttcac gcctcacttt tatggcaggg aattctgtga
     3481 aaggagcggc agaggctgcc ctagcaaaat gggaagcgga agaacgcccc gccattgcag
     3541 aattttcata ctttgcaccg cccaccactc ccatggataa agaaaccggc tattcgatgc
     3601 cgaacttcca atatgcgtat gtggcacagg ctgtggaagt ggaagtagat accgaaactg
     3661 gtcatgtgcg tgtcaaacgc atcatttctg cagatgatgt tggtactgct atcaatcccg
     3721 atctcgtggt ggcacaaatc gaaggagcgg ttgttcaagc acatggctat gccatgatgg
     3781 aagaattcaa gaccaaaaat ggacgtgttc taacagatca gttcagtacc tatctattgc
     3841 ctacaatttg ggatattcct gaaaaagttg aatctgtcct agttgagatt ccagacccga
     3901 acggaccttg gggtgcacgt ggtgttggag aattacctta tttgccagtt gccgccgcta
     3961 ttgccgctgc catccatgat gcaacagggg tctggattag tgaatttcca ttcaccccag
     4021 aacgagtgtt acgggcatta gggaaaataa aataaaaaac aaaggactga caccaaaacc
     4081 tgtcagtcct ttattggatt tcatgaattc aattttccaa gctttagccg aacttgaaaa
     4141 gaataagcaa cctgccgctc tttgtactgt ggtcaagagt gaagggtcta cgccgcgcca
     4201 tgtgggcagt aaaatgctgg tttacccaga tggtacattt attggcacag taggcggcgg
     4261 agacttggaa catcgcgtcc tcgatgaagc ttggatggcg ttaagcgatg gacaatcgcg
     4321 ccatttacat tacaccatgt ctgatccttc acgcggcgac ccaggagtat gtggaggtca
     4381 ggtggaggta tttgtggaac cgattcttcc ccccgccacc ttggtagtga tcggtgcagg
     4441 gcatgttggc aaagccgttg tccaccttgc gaagtggctg ggcttccatg tcgttgtcag
     4501 cgatgaccgt gccgagtttt gcaccccaga ggctaccccc ggtgcagatg catattactc
     4561 tgtcgaaatg gggaaattgg cagagacact caaaatcacg aatcgaactt atttggttgt
     4621 gaccagccgc gggtccagtg tagatgcgtt gggtctgcca tccctacttg aaagtgaagc
     4681 agcctatatc ggtgtcatcg ggtcaaaacg caggtgggca accacggtca aggcattgaa
     4741 agaaaacggt gtgaaggaag aattgatcgc caaagtccat tcacccatgg gcttggaatt
     4801 acaggcagaa acgccggaag agatcgctgt tagtatcatg gctgaagtgt tacttgttcg
     4861 agacaaaggc actggcaaat caatgagggc ataagatgac attaccatcc aatcaacgca
     4921 tgttccatgg aggaatacgt gcggcagcat tagcagacca actcggtgca cgtttcaaca
     4981 gcagacaaag acgcgttagt gtacattcac aaggcgatac ggctttggta caaatcggat
     5041 ccaagcatgg aactccgctt acggttcata ttgctgatac agaaggcggt gtattagtga
     5101 ccatgagtcg cgaacgaaac tggctcgaca aagccgcaga taccagtgaa atgatggaac
     5161 gggcggcgaa cagcccgctt tcgcttttag caatgattcc cgatattgtt ggcgaaatga
     5221 gccaagaaaa tatcgcccca aaggtttggg atgcaatcaa cgatctatgt tcgctcaccc
     5281 gctcacttgc aggcgaaaag aacgctccac gcaacccaaa ggtatgtact ttctgcggaa
     5341 ccgccaacga ccccgaactt gagttgtgcg tggcatgcgg cggctccctc cccaccgact
     5401 tgcctcgggt atgcccaaaa tgcagccggg gacatacatc agatgcccta ttctgccaag
     5461 catgtggaac acgcctcgta gaagggtaat tctgcaacga aacccgcaat ttcgcgtaca
     5521 taagcacata taggaaaaac ggttcttgac cgaaaaatca gatggactat aatcaaaacg
     5581 ttatttactc aaaggagaga aaccatggct agaatttttg acgtcattga atatgcaaat
     5641 gaaatgaacg acgagatcgt acaccgtttc ccagaagagg gaattggtga tttccgcatc
     5701 ggctcgcagg tgattgtgcg cgaaagccag aatgctgttt tcttccgcga cggaaacgct
     5761 ctggacgtgt tcaaggctgg tcgccatacc attgcgactg ccaacatccc gatgttgatc
     5821 gacttcattg gcaaggcgtt taatgaccg
//
LOCUS       DAOQFT010000075         5801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6951_length_5801_cov_7.267838, whole genome shotgun sequence.
ACCESSION   DAOQFT010000075 DAOQFT010000000
VERSION     DAOQFT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6951_length_5801_cov_7.267838"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLR93_03055"
     CDS             complement(<1..242)
                     /locus_tag="PLR93_03055"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNH25967.1"
                     /translation="MTETFLIIGLGNPGREYKDTRHNIGFMMIDHLAETIGARGMKVQ
                     SKAIVISGLHQERKLILAKPQTYMNLSGQSVQGLLH"
     gene            complement(257..886)
                     /locus_tag="PLR93_03060"
     CDS             complement(257..886)
                     /locus_tag="PLR93_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25968.1"
                     /translation="MLNLPIAMLKLFLILFLIFQGNTPSITSPQTGETLRGQVQIQGL
                     MDIPNFASAELAFTFDASASDPAASWFPIQTFPQPIQNPTLATWDTTAVTDGEYALRL
                     RIFLLDGTSQDVIVNTLKIRNNEPLPTPTITPTEAVFEFQPLDQTPGVSNEQNTPTPV
                     VLYSTATPLPANPASLTTSSIVTIFWQSAVLVVILFAFFSLLLRLRKNT"
     gene            1036..1410
                     /locus_tag="PLR93_03065"
     CDS             1036..1410
                     /locus_tag="PLR93_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNH25969.1"
                     /translation="MAPSRAEQLVERLRNMQAAAPDIEASAIVSVDGLIMASALQQGV
                     EEDRVSAMSAAMLSLGERISGELGRGGLEQVYIKGDKGSIVLTAIGDEAVLTAMARQE
                     AKLGMVFLEMRRAAEDLVKLVG"
     gene            1556..3166
                     /locus_tag="PLR93_03070"
     CDS             1556..3166
                     /locus_tag="PLR93_03070"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNH25970.1"
                     /translation="MATKYLFFTGGVVSSVGKGVTAAATGLLLKERGFKVAVQKLDPY
                     INVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFIDIRLSRVSNFTTGQVYAETIAK
                     ERRGDYLGGTIQVIPHITNEIKRRIGLAAKETGAEIMILEVGGTVGDIESQPFLEALR
                     QLRNEVGRENCLFIHVTWLPVIGATGEIKTKPTQHSVAALRSIGISPNVIIARADQDV
                     DQGLCDKIALFCDVEKDAVIPMKTASVLYEVPLLLDQLGVGELILNKLGLQATRKPDM
                     RPWKKLVDEVKKEKPTVNVALVGKYVELQDAYMSVREALKHAALANGVEISISWVHSA
                     DLEKDKGWDVVKEADAVLVPGGFGSRGVEGKVLAARYARENKVPYLGLCLGMQVMCIE
                     FARDVLELEDANSSEFDRSSPHPVIDLMIDQRSITDMGGTMRLGLYPCLLQPGTKAAN
                     AYSVESVDERHRHRWEFNNHYREDFEKHGMVFSGLSPDGRLVEIVELKDHPYMVASQF
                     HPEFLSRPMSPHPLFVGLLKAAKEKAGK"
     gene            complement(3224..3922)
                     /locus_tag="PLR93_03075"
     CDS             complement(3224..3922)
                     /locus_tag="PLR93_03075"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNH25971.1"
                     /translation="MKLTYKIFLLFLMSTLFLVACGGNDTAATPELSGTGAVETLVAS
                     FFATQTAMYTPPPTNTNTSTPTNTALPTPTTPSNTPPPLATWTPVIVYASSTANLALT
                     PSVTGTPPTATQNTGALGFGCNNLAIVRDVNYPSGTTVKAGEVITKTWKVQNTGSCDW
                     QYNYALVIISDEYFNSGWSRLGKDVAPGSWSEISVTLGMPNHDGTFTGSWRLADAGGN
                     AFGATLSVSIVVVE"
     gene            complement(3992..4504)
                     /locus_tag="PLR93_03080"
     CDS             complement(3992..4504)
                     /locus_tag="PLR93_03080"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNH25972.1"
                     /translation="MPENSQHGANIMPQEEHKHKEPIKDKLIPFLAYSKLNKTPVREF
                     AARLKAEGWIDPWFDEEDILPGQIWQNSVVTGVRQSHAVIIFLSKVAIASAGFYQKEI
                     KLALDTASEKPEGTIFIIPIRLNDCEVPEMLSKYQYIDYFGGLEQKEKVYSSLILSLK
                     LRAENLGIKV"
     gene            complement(4517..5197)
                     /locus_tag="PLR93_03085"
     CDS             complement(4517..5197)
                     /locus_tag="PLR93_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH25973.1"
                     /translation="MTEVTFFDPRDKLHFAFRATLFRKVIIFSLGSLFKLIMDLKVEG
                     AENLPSDGAVVLVANHVTNFDVFPMQLSIPRPIFFMAKSGLFKFPILDVALRDLGAFP
                     VYRGEKDEWALRHAARVLEQGQTLGMFPEGTRSKGKGLGVAKTGSARLALDANCPIVP
                     MAVIGTDKFFKRFPFRTKVIIKLLPPMLPKPGETPLALMDRVMFSLAKELPTEMRGVY
                     SEVPKGFE"
     gene            5317..>5801
                     /gene="eno"
                     /locus_tag="PLR93_03090"
     CDS             5317..>5801
                     /gene="eno"
                     /locus_tag="PLR93_03090"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNH25974.1"
                     /translation="MDTTIESISALEVLDSRGNPTVEVEVLLMDGSWGRAAVPSGAST
                     GEHEALEMRDGDKKRYLGKGVANAVSNVNGVIAESLFGWDASDQRGIDAEMIAMDGTA
                     NKSKLGANAILGVSLAVAKAAANSFGMPLYRYLGGVYAHVLPVPMMNIMNGGAHTSWQ
                     ST"
BASE COUNT         1562 a         1274 c         1508 g         1457 t
ORIGIN      
        1 aaatgcaaaa gtccctgcac cgattgccct gaaagattca tataggtctg aggcttggca
       61 aggatcaatt tgcgttcctg atgcaaacca gaaatgacga tggctttgga ttgaaccttc
      121 attccgcgcg cgccaattgt ctcggcaaga tggtcgatca tcataaagcc gatattgtgg
      181 cgggtgtctt tatattcacg ccccgggttt ccgagaccaa taatgagaaa agtttctgtc
      241 atgagaaaaa tccaagctag gtgttttttc gaagtcgaag aaggagggag aaaaaagcaa
      301 acaggatcac aaccaatacg gcgctttgcc aaaaaatagt aacgatggat gaagttgtca
      361 acgaggctgg gttagcgggc aaaggagttg cagtggaata taaaacgaca ggtgttgggg
      421 tattttgttc gttggatact ccaggagttt ggtcgagcgg ttgaaattca aagacggctt
      481 ctgttggggt aatggtcggt gtgggaagcg gttcattatt gcgaattttc agggtgttga
      541 caatgacatc ttgagaggta ccgtctagga ggaagatgcg taatcgcaaa gcatattccc
      601 catcggtgac ggcggttgta tcccaagtgg cgagggtggg attctgaatc ggctggggaa
      661 aggtttggat gggaaaccag cttgcggcgg ggtctgaggc ggaagcgtca aacgtaaaag
      721 ccaactctgc cgaggcaaag ttgggaatat ccatcagccc ttgaatttgg acttgtccac
      781 gcagggtttc gcccgtttgt ggggatgtga ttgaaggtgt atttccctga aaaatgagga
      841 aaagaatcaa aaacagcttg agcatggcaa tcggtaagtt taacatgaag agaaggcaag
      901 gtcaaatttt gcctattttt ctgtaattgt gagattgaaa atgaattgaa atgtgggggc
      961 aattctcgaa tgaagatttt tctggtaaac tctagccgtt catagaacgt aaacgtttat
     1021 taggagatct aagttatggc accatcccgc gctgaacaac tcgtggaacg acttcgcaac
     1081 atgcaagccg ccgccccaga cattgaagct tctgccattg tttcggtgga tggcttaatc
     1141 atggcatctg ccctgcaaca gggtgtggaa gaagatcgtg tatccgcgat gtcggcggca
     1201 atgttgagcc ttggcgaacg catctctggt gagttgggtc gcggtggttt ggaacaggtc
     1261 tatatcaaag gtgataaagg ttccatcgta ttaaccgcta tcggcgacga agccgtgctc
     1321 actgctatgg cacgtcaaga agcgaagctc ggtatggtct tcctcgaaat gaggcgagcc
     1381 gctgaagatc tggtgaagtt agtcggatga gacgtaaaag tcactccact tcaaaataag
     1441 gtaaccccga atggggaggg cataacgccc tccccattct ctttgtatat accccctcat
     1501 tcgcctttcc tgtctgtgca acagggtggg ctgtaaaacc aaggagaaga aaaggatggc
     1561 aacaaagtat ttatttttta cgggcggtgt ggtttcgtcg gtgggcaaag gcgtgactgc
     1621 ggcagcaacc ggtttgctgc ttaaggaacg cggcttcaaa gtggcggttc aaaaactgga
     1681 cccgtacatc aacgtggacc caggcacgat gtcgccgtac caacatggtg aagtgtatgt
     1741 gctcgatgat ggcgcagaaa cagaccttga tctcggtcac tacgaacgat tcattgacat
     1801 ccgcttgagc cgagtcagca acttcacgac cgggcaggtc tatgctgaga ccattgccaa
     1861 ggagcgtcgt ggtgactatc tcggcgggac gattcaggtc attccgcaca tcacgaacga
     1921 gatcaaacgt cgtattgggt tggcagcgaa agaaaccggt gccgaaatca tgattctcga
     1981 agtaggcggt acggttgggg atattgagtc tcagccgttt cttgaagcgc ttcgccaatt
     2041 aagaaatgag gttgggcgtg aaaactgcct tttcattcac gtcacttggc tgccagtgat
     2101 cggcgcgacc ggtgaaatca agaccaaacc gactcaacac tctgtggcgg ctctacgctc
     2161 cattggtatt tcgccaaatg tcatcatcgc ccgcgccgac caagatgttg accaaggctt
     2221 atgcgacaaa attgccttgt tctgtgatgt agaaaaagat gcggttattc caatgaagac
     2281 cgccagtgtc ctttatgaag tgcccctgct tttagaccaa cttggtgtgg gcgaactcat
     2341 tctcaataaa ctgggtctac aagctacacg taagccggat atgcgcccat ggaagaaatt
     2401 ggtggatgag gttaagaaag aaaagccaac ggtcaatgtc gcattggttg gcaaatacgt
     2461 tgaattgcaa gatgcctata tgtcggttcg tgaggcgtta aaacatgccg ccctcgcaaa
     2521 tggcgtagag atttccatta gctgggttca ttcagcagac cttgaaaagg ataaaggctg
     2581 ggacgtggtt aaagaagcgg acgctgtact cgtccccggc gggtttggtt cgcgcggcgt
     2641 agaaggcaaa gtgcttgcag cacggtacgc ccgcgaaaat aaagtccctt atcttgggtt
     2701 gtgtctcgga atgcaggtta tgtgtatcga atttgcacgt gatgtgctcg aacttgaaga
     2761 tgccaactcc tcagaatttg accgtagttc tccgcacccg gtcattgatt tgatgatcga
     2821 ccagcgttcc attactgata tgggcggaac catgcgcctc ggactttacc catgcctgct
     2881 ccaaccaggg acgaaagccg ccaacgccta tagcgtcgaa agtgtggacg aacgccatcg
     2941 tcaccgctgg gagttcaaca atcattaccg agaagacttt gaaaaacacg gcatggtgtt
     3001 ctctggactc tcccccgatg gtcgcttagt tgaaattgtt gaattgaaag atcatcctta
     3061 catggtcgcg agtcagttcc accctgaatt cttgtcccgc ccaatgagcc cacatccgct
     3121 atttgtggga ttactaaaag cagcaaaaga aaaagccggc aagtaattac taaaacaccc
     3181 tccgaaattt ggagggtgtt ttagtaaaga aaattatttt cttttattcc actacgacaa
     3241 ttgaaacact caatgtcgca ccaaaagcgt ttccgcctgc atctgccaaa cgccacgaac
     3301 cggtgaaagt gccgtcatga ttaggcatcc ccaatgtgac cgatatttct gaccaactgc
     3361 ctggagctac atccttccca aggcggctcc aacctgagtt gaagtattca tcgctaataa
     3421 tcactaaagc gtagttatat tgccaatcac atgagcctgt gttctgaacc ttccaggttt
     3481 tggtaataac ttcgcctgcc ttgactgttg taccgcttgg atagttcaca tcgcgaacaa
     3541 tagctaaatt attgcagcca aaacccaaag cccctgtatt ttgagttgcg gtgggaggcg
     3601 tccccgtcac ggatggagtc aaggcaaggt tggcagtaga actggcatac acaataaccg
     3661 gcgtccatgt ggcaagcgga ggcggagtat tcgacggtgt tgtaggtgtt ggtaaggcag
     3721 tgtttgttgg agttgaggta ttcgtattgg ttggtggcgg ggtgtacatg gcagtttgtg
     3781 tagcaaagaa gctagccact aatgtttcca ctgctccagt tccagagagt tctggggtag
     3841 ccgccgtatc attcccacca caggcaacta aaaaaagtgt ggacattaaa aacagtaaaa
     3901 atatcttata tgtgagtttc attgagataa cctttctatg taatgattat aaaatgaaac
     3961 ttcaattcgg atttagaacc acagaatgga tttaaacctt gatccccaaa ttttcagctc
     4021 tcaattttaa agaaaggatc aaactggaat acactttttc tttttgttcc aagccaccga
     4081 aataatcaat atattggtat ttactcaaca tttcagggac ttcgcagtca tttaggcgaa
     4141 taggaataat gaaaatcgta ccctctggct tttctgaagc ggtatcaagg gctaacttga
     4201 tctctttttg gtaaaaacca gccgaagcaa ttgcaacctt tgaaaggaaa ataataacag
     4261 catgggactg tctcaccccg gtgacaacgg aattttgcca aatttgcccc ggcaaaatat
     4321 cttcttcgtc aaaccaaggg tcaatccagc cctcggcttt gagacgggct gcaaattcac
     4381 gaacaggggt tttattgagc ttggaatacg caagaaatgg aatgagtttg tctttgattg
     4441 gttctttgtg tttatgttct tcttgcggca tgatgttggc tccgtgttgt gagttttcag
     4501 gcattatatc gcagcgttat tcaaatccct tcggcacctc ggaatagact ccgcgcattt
     4561 cagtgggcaa ttctttagca agcgaaaaca taactcgatc catcaatgca agtggggttt
     4621 caccaggttt tggcaacatg ggcggcaaga gtttgattat tactttagta cggaacggga
     4681 atcgtttgaa aaacttgtcg gtgccgatca cagccatggg aacgatggga cagtttgcgt
     4741 caagcgcgag gcgggcagag ccggttttgg cgacgccaag tccctttcct ttggaacgtg
     4801 tcccttcggg gaacatacct aaagtctgcc cttgttctaa aacacgggca gcatgccgca
     4861 gtgcccattc atctttctcg ccgcgataaa cggggaaagc gcctaagtca cgcagggcaa
     4921 catcaaggat ggggaatttg aacaaaccag atttcgccat gaaaaagatg gggcgcggaa
     4981 tgctcaactg catggggaat acatcaaaat ttgtgacgtg attcgcaacc aaaacaacgg
     5041 caccatctga gggcagattt tcggcgcctt ctaccttgag gtccataatg agcttaaaaa
     5101 ggctgccaag tgaaaaaatg atgacttttc gaaatagagt cgctcgaaag gcaaaatgga
     5161 gtttatcgcg agggtcaaaa aaggtgactt ctgtcaagat ttcatctctc aaatttggga
     5221 ttatactaag gcgaaaatta aaaatcaggg gcgacctgtc ttgggatatt aacaccatca
     5281 agcaaaaatc gtcgccttca tttaggagca aattaaatgg atacaacaat tgaaagcatc
     5341 tccgcgctag aagttcttga ttcacgcgga aaccccactg tagaagtgga agttttattg
     5401 atggacggct catggggacg cgctgcggtt ccctccggtg cctcaaccgg tgagcacgaa
     5461 gcgctcgaaa tgcgcgatgg cgataagaaa cgctatctcg gcaaaggcgt agccaatgct
     5521 gtctcaaacg tcaatggtgt gatcgccgaa tccttgttcg ggtgggatgc atctgaccag
     5581 agaggcatcg atgctgaaat gatcgccatg gatgggactg caaacaagtc taaactcggc
     5641 gcgaatgcaa tcctcggtgt gagtttggcg gttgccaagg cagcggcaaa ttccttcgga
     5701 atgcctcttt atcgctacct cggtggcgtg tatgcgcacg tcctgccggt gcctatgatg
     5761 aacatcatga atggtggtgc acacacaagc tggcaaagca c
//
LOCUS       DAOQFT010000076         5793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_6969_length_5793_cov_5.735622, whole genome shotgun sequence.
ACCESSION   DAOQFT010000076 DAOQFT010000000
VERSION     DAOQFT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_5793_cov_5.735622"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLR93_03095"
     CDS             <1..468
                     /locus_tag="PLR93_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNH25975.1"
                     /translation="LAEKQTQIIIQFKEPPTTMFPMQTMKPNMLVLYLQPDEGVHLRF
                     EAKAPDTVNETRSVDMEFHYDDAFGKSSIPESYERLLLDALQGDASLFTRADEVETAW
                     SLIDPILQTWETHQTPPLAVYKPGSWGPPEGYDLLARDGRRWLNEEASMMLEK"
     gene            663..1784
                     /locus_tag="PLR93_03100"
     CDS             663..1784
                     /locus_tag="PLR93_03100"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH25976.1"
                     /translation="MKSPKLIITILLALALTACGNASAATPESTPIPVVEADDTIIAE
                     GKLEPITYTEISLNTSGLVSELPVEEGDRVEAGDVLAVVRSENAQSLEDAQAKATQEL
                     TEAYKEYRDAQSRLDDFDVPARWNGMTPTQAIASSLEKLDVARTEFEPYKHLSEKLIK
                     YEEPKPDFSEMTQEEMEFYEASLENHKNDDRPLNGEARAKKKALDEAWELYRISIRWL
                     ELESNYQNAKVRLANAQADFDALQDTDFSLDTAALRAILANAELRAPHSGVITDLNLK
                     VGEFATDAAPVATIADTSKWLVKTSDLTEIDVVNLEEGQPVTVKLDAIPGKEFKGNVL
                     SIGQNYSENQGDVVYEVTILLSEFDPSMRWGMTAVVTFE"
     gene            1830..2972
                     /locus_tag="PLR93_03105"
     CDS             1830..2972
                     /locus_tag="PLR93_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH25977.1"
                     /translation="MAISFFKTKAEDSDSAERPIIDIRSVNKFYKTAVGDYHALSNVD
                     LQIQAGEFVSIIGKSGSGKTTLLNMITGIDRPSNGEVWVNGTGVHTLNENKMARWRGK
                     NLGVVFQFFQLLPMISVIENIMLPMDFCRMYSPAERRKRAMDLLELVELADHAHKLPT
                     ALSGGQQQRVAIARALANDPPVIIADEPTGNLDSKTAESVFVLFNDLVAKGKTIIIVT
                     HDSAMAKRTHRTALIADGEIVNEYVAKAMPTLSHTQLLQATKSTTPMTFEAGAMILSE
                     GTNADTFYIVSHGTVEVILPRPNQSDVVAVQLGPGKVFGEMEFFHEKKHRASIRASEA
                     GPVEVLAIGYDKLTELLSQSDVTREALREMAGKHNGENLSWRGVSS"
     gene            2969..5362
                     /locus_tag="PLR93_03110"
     CDS             2969..5362
                     /locus_tag="PLR93_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH25978.1"
                     /translation="MIASRWKKVWSDFWSNRGRTFLTIATIAVGTFAVGFNSNLGLYM
                     NESMDGDFLSANPSEAEIYASGLNDDLVKKAREVPGVEAVEGYSSVQGQLIRPDGTKI
                     DISFTAAETPNALTLNFLKPAMGETSIPSYGKKEVILDASASSLGYTAGDTIVIEFDN
                     GKRRELTFTGYMHAVTGFPYNLAQQVNAYVTPDTLEWLGGNINQYGRLSISVSEKQTD
                     ADHVTAVAQAVADELKRAGSTVYFVSVYQPGHHFAYSITQGVFFVLGVLGYLTVLLSG
                     FLIVNTITALMTQHTRQIGIMKATGGSAWQIFFMYLVLIMSFGIGALLLAVPLANIAA
                     QTIGSGMASWLNFYPTDYKGYSITLIQQVIVAILVPLLAAILPIYNSVRITVREALSD
                     YGLGGNVKTKEETVGKNSVLVPRPIRISLRNTFRRKTRLGLTLFTLVLGGAVFIAVYN
                     LWASFDKTIEDIQGYFLADINISFDKGYRYDKVASIATSIPGVEGAEGWLEWTGTLEM
                     NDDEAGTQILFVAPPSNSKLIDPIITSGRWLKPGDENAIVIGNHLLQIFPDLKVGDWL
                     NIDIDGKKTKWQIVGTYSITGNVSPPLLYVNYEYLSVLVGEPEMAYSLRIVTSQHDSA
                     TQNRINDQIQAAFEERGIQISSTQLGADFIRDQKAQTDVLVYFMLVMASLIAFVGGLG
                     LMGTMSINVLERTREIGVMRAIGASNGDIQAIVITEGMVIGILSWAISIFISIPITNV
                     LCYGVGMAILTAPMPAIYGMTGILAWLIFTLVLGTLASALPARRASKLTVRDTLAYE"
     gene            5479..>5793
                     /locus_tag="PLR93_03115"
     CDS             5479..>5793
                     /locus_tag="PLR93_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25979.1"
                     /translation="MKTKKWLWWTLTILLTLVVLTGVAIAGFRLGAMHSATLTAEGTS
                     AFAFHHGHDFSGNMTHEGFGMMRGSERGHGFGGRGGFGFFGPLFGLIQLAVFGGLVWL
                     AFS"
BASE COUNT         1560 a         1387 c         1386 g         1460 t
ORIGIN      
        1 ctcgccgaaa agcaaaccca gatcatcatc caatttaaag agccgcctac caccatgttc
       61 cccatgcaaa ccatgaagcc gaacatgttg gtgctttact tgcaacccga tgaaggcgtc
      121 cacttgcgtt ttgaagccaa ggcgcctgat acggtcaatg aaacccgctc cgtagatatg
      181 gaattccact atgacgatgc ttttggcaag tcgtccattc ctgaatccta tgagcgcctt
      241 ttgctcgatg ctctgcaagg cgacgcgtct ctctttaccc gcgcagatga agtggaaaca
      301 gcttggtcgc tgattgaccc cattctccag acgtgggaga cgcatcagac tccgccgttg
      361 gctgtttaca aacccggcag ttggggtcca cccgaaggct acgacctgct cgctcgggat
      421 ggtcggcgct ggctcaatga agaagctagc atgatgttgg aaaaataaaa cagttctcaa
      481 gggtgggaaa aagaatagaa aagtaatacg gaaagagcac aattctttta gattgtgctc
      541 tttttttgtg caaccaaact tcccatggcg tcgtaagtaa acacgaaaag ttcaccgaaa
      601 tttgaaactt ttctttatat atcctgtctt aacaaataat caatccttca aaggaagcgg
      661 acatgaaatc gcctaaactt atcatcacga tcttacttgc actagctctc acagcctgcg
      721 gaaatgcctc agcagcaacg ccagaaagca ctcccattcc cgtcgttgaa gcagacgata
      781 ccattatcgc cgaagggaaa ctggagccta ttacctatac agaaatctcc ctaaacacga
      841 gcggactggt cagcgaatta cccgtggaag aaggagatcg tgtggaagca ggtgatgtgc
      901 ttgctgttgt gcgttctgaa aatgcgcaat ctctcgaaga tgctcaagca aaagctaccc
      961 aagaactgac cgaagcctac aaagaatatc gcgacgccca aagccgcttg gatgattttg
     1021 atgtccctgc acgctggaat ggtatgacac ctacccaagc gattgcttcc tctctcgaaa
     1081 agttggatgt tgcccgtaca gaatttgagc cgtacaagca tctttcagag aaattaatca
     1141 agtatgaaga acccaaaccc gatttctctg aaatgactca ggaagagatg gagttctacg
     1201 aagccagcct tgaaaatcat aaaaacgatg accgtccctt gaatggcgaa gcgcgcgcaa
     1261 agaaaaaggc actcgatgaa gcttgggagt tataccgcat ctctattcgc tggctggaat
     1321 tggaaagcaa ctatcaaaat gcaaaggttc ggcttgccaa tgctcaagca gactttgacg
     1381 ctctgcaaga tacagatttc tcactcgata ccgccgcctt acgtgccatt cttgcaaacg
     1441 ctgaattacg tgcccctcat tcaggtgtca ttacagactt gaatctcaaa gtcggtgaat
     1501 ttgcgactga tgcagcaccc gtagcaacta ttgcagatac gtccaaatgg ctcgtcaaaa
     1561 ccagtgacct gaccgagatc gatgtggtca accttgaaga aggacagccc gtcaccgtta
     1621 agttggatgc cattcccggc aaagaattca agggcaatgt actctccatc ggtcagaatt
     1681 actctgaaaa tcagggcgac gtggtctatg aagtcaccat tctcctaagt gagttcgacc
     1741 cgtctatgcg ctggggcatg accgctgtcg ttaccttcga ataaagtagt caacatggca
     1801 tatagatgtc gtgttgaagg aaattactaa tggctatatc cttcttcaaa acgaaagcgg
     1861 aagacagcga cagcgcagaa cgcccgatca ttgatattcg cagtgtgaac aagttctaca
     1921 aaaccgctgt tggcgattat cacgcgctca gcaatgtaga tctgcaaatt caggcaggcg
     1981 agttcgtcag cattatcggc aaatcgggca gcggtaagac aactctattg aacatgatca
     2041 ccggcattga tcgcccatca aatggcgaag tatgggtcaa tggcacaggt gtgcatacgc
     2101 tcaatgaaaa taaaatggca cgttggcgtg gaaagaatct gggcgttgta ttccagttct
     2161 ttcaactttt gccgatgatc tctgtgatcg aaaacattat gttgcccatg gatttctgcc
     2221 gcatgtactc ccctgccgaa cgccgcaaac gcgccatgga cttgcttgaa ttggtggaac
     2281 tggcagacca cgcacataaa ctccccaccg cgctttcagg tggacaacag caacgtgtag
     2341 cgatagcgcg tgcgcttgct aatgatccgc ccgtgattat tgcagatgaa ccaacaggca
     2401 accttgactc aaaaacagcc gagtctgttt ttgtgctatt caatgacctt gtcgcaaaag
     2461 gcaaaaccat tatcatcgtc acacatgata gcgccatggc aaaacgcacc catcgcaccg
     2521 cactcatcgc agacggcgaa attgtcaacg agtacgtggc aaaggcaatg cccacacttt
     2581 ctcatactca acttttacag gcaacaaaat ctaccactcc catgaccttc gaagcaggtg
     2641 ccatgatcct ttctgaaggg acaaatgcag atacgttcta catcgtctca catggaacgg
     2701 tggaagttat tttgccgcgc ccgaatcaat cggatgtcgt ggcggttcaa ctcggacctg
     2761 gaaaagtgtt cggcgaaatg gaatttttcc acgagaagaa gcaccgtgct tccatccgcg
     2821 cctcggaagc gggacctgta gaggttcttg ctatcgggta tgacaaactc acagaattgc
     2881 tgagtcagtc tgacgttacg cgtgaagcgc tgagagagat ggcaggcaaa cacaatggcg
     2941 agaatctatc ctggcgggga gtctcatcat gattgccagt cgttggaaaa aagtctggtc
     3001 tgatttttgg agcaaccgcg ggcggacctt cttaaccatt gccacaattg ctgttgggac
     3061 gtttgctgta gggttcaata gcaatcttgg cttgtatatg aatgaaagta tggacggaga
     3121 ttttctgtct gccaaccctt ctgaggcaga gatttacgct tcgggcttaa atgatgactt
     3181 agtcaagaag gcgcgcgaag tacccggtgt tgaagcagtg gaagggtatt ctagtgtaca
     3241 ggggcaactt attcgccctg atggaacgaa aatagatatc tctttcactg cggcagaaac
     3301 ccccaatgcg ttgacattaa attttctcaa acctgcaatg ggagaaacga gtattcccag
     3361 ttatggcaaa aaggaagtca tccttgatgc tagcgcttcg tctctgggat atacagcggg
     3421 agatacgatt gtcatcgagt tcgataatgg caaacgccgc gaactaacct ttactgggta
     3481 tatgcatgcc gtgacgggtt tcccctacaa cttagcgcaa caagtaaatg catacgttac
     3541 gccagacaca ttagagtggt taggcggaaa cataaatcaa tatggaagac tctcgatcag
     3601 tgtttctgaa aagcagacag acgcagacca tgtcactgct gtagctcagg cagttgctga
     3661 tgagcttaag cgagcagggt ctacggtgta ttttgtaagt gtgtatcaac cggggcatca
     3721 ctttgcctac agcattacac aaggtgtgtt cttcgtgttg ggcgttttgg gatatctcac
     3781 cgtattgctt agcggatttt tgatcgtcaa caccatcact gcattgatga ctcaacacac
     3841 acgtcaaatc ggcatcatga aagcaaccgg cggaagtgcc tggcagattt ttttcatgta
     3901 tttggtgctc atcatgagtt ttggcattgg agcgctcttg ctggctgttc cgcttgccaa
     3961 tattgctgcc caaaccatcg gaagtggaat ggcatcttgg ttgaactttt acccaacaga
     4021 ctataagggc tattccatta cattgattca acaagttatc gttgcaatct tggttccgtt
     4081 actagccgcc atcctgccaa tctataacag cgtcagaatt acagttcgtg aagcattgag
     4141 cgattatgga ttaggcggta acgtgaagac aaaagaagag actgtaggca aaaattcggt
     4201 attggtccca cgcccgatcc gcatttcctt gcgcaataca tttcgaagaa aaacccgcct
     4261 aggtctaacc ctcttcacac tagtacttgg cggggctgta ttcattgctg tttacaactt
     4321 gtgggcatcc tttgataaaa caatcgaaga tattcaaggg tactttctgg ctgacatcaa
     4381 catttcgttt gataaaggct atcgatacga taaggtcgcc agcattgcca caagcattcc
     4441 tggagtagaa ggtgcagagg gatggctgga atggaccggc acccttgaaa tgaatgacga
     4501 cgaagccgga actcaaatat tatttgtggc gcctccttct aactcgaaat tgatcgaccc
     4561 cattatcact tctggtcgct ggttgaaacc cggtgatgag aatgcgattg tgatcggcaa
     4621 tcatctcttg caaatctttc cagacctgaa agttggtgat tggctcaaca ttgatattga
     4681 cggtaaaaag acaaaatggc aaattgtcgg cacttatagc atcactggca acgtcagccc
     4741 gccattgtta tatgtgaatt atgagtactt aagcgttttg gtcggcgaac ctgaaatggc
     4801 atactccctc cgtatcgtca caagtcaaca tgactctgcc actcaaaacc gtatcaatga
     4861 ccagattcaa gcggcattcg aagaacgcgg tattcagatc agctccacgc aattaggcgc
     4921 agatttcatc cgcgaccaga aagcacagac agacgttctc gtatatttca tgctggtcat
     4981 ggcatcattg atcgctttcg tcggtggttt aggcttgatg ggcaccatga gcatcaatgt
     5041 gttggaacgc actcgtgaaa tcggtgtgat gcgggcgatt ggcgcaagca acggcgatat
     5101 tcaggcgatt gtcatcacag aaggaatggt aatcggcatc ctgagttggg caattagcat
     5161 cttcatttca attcccatca caaatgtctt gtgctatggg gttggcatgg caattctcac
     5221 cgctcccatg cctgccattt acggcatgac cggcatcctt gcatggctga tcttcactct
     5281 tgtgttgggc actcttgcca gtgcgttacc tgcacgccgc gcctcaaagt tgactgtgag
     5341 agatacgctg gcttacgaat aaagtctata ggtacgttat ttacaatctc tgcgaacaca
     5401 caactttcac acatttcctc cattgctttg taatacaggt gtgaaaaaat agagtcaagt
     5461 ttacaaatgg aggtaaacat gaaaacaaaa aaatggcttt ggtggactct caccatcctt
     5521 ctgactcttg tggtgttgac aggcgttgca attgcaggct tccgcttggg tgctatgcat
     5581 agcgccacgc tcaccgcaga aggaacttcc gctttcgcgt tccatcacgg acatgatttc
     5641 agcggcaata tgacacacga aggcttcggc atgatgcgcg gctctgaacg tggacatggt
     5701 ttcggcggac gtggcgggtt tggctttttc ggtccgctgt ttgggctgat tcaactcgcg
     5761 gtgttcggcg gcttggtttg gttggcattt agc
//
LOCUS       DAOQFT010000077         5776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7000_length_5776_cov_6.479986, whole genome shotgun sequence.
ACCESSION   DAOQFT010000077 DAOQFT010000000
VERSION     DAOQFT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7000_length_5776_cov_6.479986"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLR93_03120"
     CDS             <1..240
                     /locus_tag="PLR93_03120"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH25980.1"
                     /translation="PAPTAATGGAIKVRTVAEAVSLRKQPVVSDATLIKRVPLGTEFT
                     ITEPGGEAKIGKNDQWIKVKDASSEGYVAAWFVAR"
     gene            258..1568
                     /locus_tag="PLR93_03125"
     CDS             258..1568
                     /locus_tag="PLR93_03125"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HNH25981.1"
                     /translation="MENQTQEPNPILQIAWTRFAQMDAYSSRRTKNHLNFRRWLGILS
                     ILATLFAIIVSLFPSDNPSLIVVVFKFLLIMTPILSSGLAVYGSKFLSSGDWLVTRAG
                     AEEILKEIYTYRTVLKNSRTRDAWLENRLAEIQRSVFRGLNGELTIPPFEGYLPPHYN
                     QSDPYSDPGFSDLIGDEYFQYRLESQLGWHVRKVNKFQRERVRLQIFIILSGVFGSIL
                     AVILPVWTALAASFTAVFLGWQELRNLDSVVRNYSKVVMELTLLYDHWKNLKPGQKTP
                     SEFYKMVRSTEDILWSQNVEYIKAMQEALKESDLEEEASLVNRVIKEARESDRRLKQS
                     MEDAVVEMTTEKLHEAEGKLSETFEEALNTLAKEASSEIVQAELAAMREAIQEFAENA
                     WQGLSTSLKEIADEYKDIDVGTDTPRDVLNSLLARYPKTSEVKG"
     gene            1597..3912
                     /locus_tag="PLR93_03130"
     CDS             1597..3912
                     /locus_tag="PLR93_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNH25982.1"
                     /translation="MTGSTETAQKAETTNTPATPAQAQPAVPGTENKGVPIIGPAGEP
                     AKNVDDSTVAYKEVDKSSLVVEVRPHAFIVMPFGKKKGGDGSLYDFNAIYKQLIKPAL
                     EEAGFEPFRADEETASGDILTDMFQELLLADLVLCDLSIDNANAFYELGIRHAFRKRG
                     VMHIQAGRAYMPFDIFNVRTLPYHVTPDGVPDAAFLKTDIQAIARMAKDTWASDRDAI
                     HSPIFNLLQGLKEPERKSLRTPLATGFWREYNEWKQRVTVAQRQKRIGDILLLTEEIS
                     NPLIKEEAIGEAGNALANMGRNELALVQYRKGLEVNSGNLDFRRKEAFHLNRLGRVDE
                     AIVKIESLLTDFPNDSEAISYLGRIYKEMWADSWKQIPDKNKRLKAAFDAYHWLIKAI
                     DTYLKGYRIDLNNHYPGVNALTLSSIAIHLADKFDDKKDPDPDITRIRDEFVELKGAL
                     IFALEKQVSADNADYWTLISLAELRALTADNTATVVRSYRKALTASRRNMFFLQASLS
                     QLEILKALDMRTTFVEAGISILDEEIARISGDKQVSAAKGNNKNGRAPEGQVFLFAGY
                     MIDYPGKNKKTFPANRERDIREDIEKLLTKFNAGPGDIAILAGLSAGSEILFAEVCAE
                     KGIHVNVHLPLPESRYIREFVSIGGEQWVDRFYKICSHPDVDEFYQLEQVGSPKKNGD
                     PYERNNRWALYSSLVRGISKTTLIAVWNNVGGRAKDRDEYLTQHMVDLMRETGGKIEA
                     INTTKYLFEGTAVPANATKPPAPKKSKSSKK"
     gene            complement(3936..4406)
                     /locus_tag="PLR93_03135"
     CDS             complement(3936..4406)
                     /locus_tag="PLR93_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25983.1"
                     /translation="MTEPILLTSESPILSELNFKPYRSSVVRKVKPFLPAQNEPQSID
                     VQTPWGATLTAKHGDMLLSELDAPNDVWPVNGEIFSETYMMIERGYCVKSAVTMLVPM
                     IDVTKGDEEQHVTIETMEGPQTVRAGDFYLARGVKGEIWSIPKEKVASIMKPVE"
     gene            4650..>5776
                     /locus_tag="PLR93_03140"
     CDS             4650..>5776
                     /locus_tag="PLR93_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25984.1"
                     /translation="MTDANENQTDKKLEASGNPIPVPRETKAAVSTDNNQVMDSSATS
                     NPALPAIKAPGDSSSKAESVPSGTEPKAPKQPRVSDDRDYDIDGSNKDKSLHPEVKPH
                     VFVVMPFGNKKGADGHPFDFNAIYKTLIRPAIEDAGFEPFRADEETASGDILTDMFQE
                     LLLADMVICDLSIDNANAFYELGIRHALRKRGVVHIQAGRAYMPFDVFNVRTLPYHIT
                     SEGLPDPAYIRSDIKAIARLLKDTWASDRELVHSPIYNLLTGLKEPSRKSLQTPLATG
                     FWREYEEWKDRVTIAQRNKHIGDILLLTEEIRNPLIREDAVAEVGGALARLGRNELAL
                     KQYREGIDVNGENLEFRREEAFHLNRVGRVNEAIVKLESIL"
BASE COUNT         1617 a         1435 c         1286 g         1438 t
ORIGIN      
        1 cctgccccta ctgcagcaac tggcggcgcg attaaagtcc gcaccgtggc agaagccgtc
       61 tctttgcgca aacagcccgt cgtcagtgat gcaactctca tcaagcgtgt gccgcttggt
      121 actgagttca ccatcaccga acccggtggc gaagccaaaa ttggcaagaa cgatcagtgg
      181 atcaaggtca aggatgcgag cagtgaaggg tatgtcgcag cttggttcgt agctcgttag
      241 gcataaagag gatactcatg gaaaaccaaa ctcaagaacc caatcccatc ctccagattg
      301 cgtggacccg ttttgcgcag atggatgcct attcgtcgcg tcgcaccaag aatcacttga
      361 acttccgccg ttggctcggc attctcagta ttctggcgac cttgttcgcg atcattgttt
      421 cactattccc ttcagacaac ccatcgctaa ttgttgtggt cttcaaattc ctgttgatta
      481 tgacccccat cctttcttcc gggttagcgg tctatggaag caaattcctc tccagcggag
      541 attggctggt cacacgtgct ggtgctgaag aaattctcaa agaaatctat acctatcgca
      601 ccgttctaaa aaattcacgc actcgcgatg cctggttgga aaatcgcttg gctgaaattc
      661 aacgttcggt ctttcgcggt ttgaacggcg aattgaccat cccgcccttt gaaggctatt
      721 taccgcctca ctacaaccaa tcagacccct atagcgaccc cggttttagc gacctaatag
      781 gcgatgaata ttttcaatat cgccttgaat ctcagcttgg ttggcatgtc agaaaagtca
      841 acaagttcca gagagaacgt gtgcgcttac aaatctttat catcctctcc ggtgttttcg
      901 gctccatttt ggctgtcatt ctcccagttt ggacggcact cgccgcttcg ttcaccgctg
      961 ttttcctcgg ctggcaggaa cttcgtaatc tagactcagt tgtccgcaat tacagcaagg
     1021 ttgtcatgga gttaactctc ctctacgatc attggaagaa cctcaaaccc ggtcaaaaga
     1081 ctccttcaga attctacaag atggttcgtt ctacagaaga tatcctctgg agccagaacg
     1141 tggaatacat taaagccatg caagaggcgc tcaaagagtc tgacctcgaa gaagaagcca
     1201 gccttgtcaa ccgcgttatt aaagaagcaa gagaatccga ccgccgcttg aaacagtcta
     1261 tggaagatgc agtggtagaa atgaccactg aaaagctgca tgaagctgaa ggtaagttaa
     1321 gcgagacctt cgaagaagct ttgaacacgt tggcgaaaga agcctcttct gaaatcgttc
     1381 aagcagagct tgctgcaatg cgcgaagcta ttcaagaatt tgctgaaaac gcatggcagg
     1441 gactttctac ctccttgaaa gaaattgcag acgagtacaa ggatatcgat gtcggtaccg
     1501 atactccgcg tgatgttctc aacagcttgc ttgcccgtta cccgaagacc agcgaagtca
     1561 agggatagag tcatatccgc ttcataggag attacaatga ccggctcaac agaaactgca
     1621 cagaaagctg aaacgaccaa tacaccagca accccggcac aagcacaacc tgccgtgcct
     1681 ggtactgaaa ataaaggtgt ccccattatt ggtcccgctg gagaacctgc gaaaaacgta
     1741 gacgactcta ccgtggcata taaagaagtg gacaaatcca gcctcgttgt tgaagttcgt
     1801 ccgcatgcat tcattgttat gccttttgga aagaaaaaag gcggcgacgg ctctctttat
     1861 gactttaacg ccatttacaa gcaactgatt aagcctgcgt tggaagaagc cggcttcgaa
     1921 ccttttagag cggatgaaga aaccgcctcg ggcgacatcc tcaccgatat gttccaagag
     1981 ttgctgcttg ccgatctagt gctgtgtgac ttaagtattg ataacgccaa tgctttttat
     2041 gaactaggca tccgccatgc gttccgtaaa cgcggcgtga tgcacattca agctggtcgc
     2101 gcctacatgc cgttcgatat cttcaatgtg cgcacccttc cctatcatgt cacccccgat
     2161 ggcgtgcccg acgccgcatt cctcaagaca gacattcaag ccattgcccg tatggcaaaa
     2221 gacacctggg cttctgaccg agatgcaatt cacagcccaa ttttcaactt gctccaaggg
     2281 ctgaaagaac cggaacgaaa gtcgcttcgc actccgctgg caactggatt ctggcgcgaa
     2341 tacaacgaat ggaaacaacg tgtaaccgtc gcacagcgcc aaaaacgcat cggcgacatc
     2401 cttttactca cagaagagat cagcaaccca ctaatcaagg aagaggcgat tggtgaagct
     2461 ggtaatgccc ttgcaaatat gggtcgcaac gagttggcac tcgttcagta tcgcaaaggc
     2521 ttggaagtca actctggcaa tctcgatttc cgtcgtaaag aagctttcca cctcaaccgc
     2581 cttggtcgtg tggatgaagc catcgtcaag attgaaagtc tgcttaccga ttttccaaat
     2641 gatagcgaag ccatctcata ccttgggcgc atctataaag aaatgtgggc agattcttgg
     2701 aaacaaattc ccgataagaa caaacgcctt aaagccgctt tcgacgccta tcactggctt
     2761 atcaaagcga tcgatacata tctcaaaggc tatcgtatcg acttgaataa tcactacccc
     2821 ggcgtcaatg cattgactct ttcttcaatc gccattcacc tcgctgacaa gttcgatgat
     2881 aagaaagacc ctgaccccga tatcacccgc atccgcgatg agtttgttga gctaaaaggc
     2941 gctcttattt tcgcactgga aaaacaagtg agtgctgaca atgcagatta ctggactctg
     3001 atctcattag cagaattacg cgcccttaca gcagataata cagctaccgt agtgcgttca
     3061 taccgcaaag cgctcacggc atcgcgtcgg aatatgttct tcttgcaggc atcgctttcc
     3121 cagctagaaa ttctgaaagc cttggatatg cgtacaacct tcgtagaagc tggcattagc
     3181 attttagacg aagaaattgc ccgcatctct ggcgataagc aagtctccgc tgctaagggg
     3241 aacaacaaaa acgggcgcgc gcctgaaggg caagtcttcc tttttgctgg ctacatgatt
     3301 gattatcccg gcaaaaacaa gaagaccttc cctgcgaata gagaaagaga tattcgtgaa
     3361 gatattgaaa agttattaac caagttcaat gcaggtcctg gcgatattgc tattttggct
     3421 gggttatcag caggtagtga aattctattt gcagaagttt gtgcagaaaa aggcattcat
     3481 gtcaatgttc atttgccgct cccagagtca agatacatcc gcgaatttgt ttcaatcggt
     3541 ggcgaacaat gggtagaccg tttctacaag atctgcagtc atcctgatgt agatgagttt
     3601 tatcagcttg aacaggttgg ctcgcccaag aaaaatggtg acccgtacga acgcaataat
     3661 cgttgggcgc tttactcttc cctcgtgcgt ggcatttcca aaacgacact tattgctgtt
     3721 tggaacaacg ttggcggacg cgcaaaagac cgcgatgagt atctgaccca acatatggta
     3781 gatctcatgc gcgaaactgg tggcaagatc gaagcaatca acacgaccaa atatcttttc
     3841 gagggtacag cagttccagc gaatgcaaca aaaccacctg ccccaaaaaa atcgaaatct
     3901 tccaagaaat aaaagcaaaa atgaaacccg tcgagctact cgacgggttt cattatggat
     3961 gctacttttt ccttcgggat agaccaaatt tcacctttta ccccacgtgc taaatagaaa
     4021 tcgcctgctc gaaccgtttg tggtccttcc atggtttcaa tcgttacgtg ttgctcttca
     4081 tcaccctttg tgacatcgat catcggaaca agcatagtca ctgcgctctt cacgcaataa
     4141 ccacgttcaa tcatcatgta cgtttcagag aaaatctctc catttacagg ccatacatca
     4201 tttggagcat ctaactcact aagaagcata tcgccgtgtt tagcagttaa ggtggctccc
     4261 caaggggttt ggacatctat cgactgcggt tcattttgag ctggtagaaa gggttttact
     4321 tttcgtacaa cagagctacg atagggttta aaatttagct cacttaaaat tgggctttca
     4381 gaagtcaata atatgggttc cgtcatttgc tctccatagt ttttcggtgt caaccgtcgg
     4441 aaagacatca tatcgctttt tgctgtaaaa ctcactccta ctttaatact agcatctaaa
     4501 cgaaattgcc ttcactttgc agaagagatt ataccaaagc aggtttccac atctatctgc
     4561 tcaaaaaagc catatccttg atacaattta ggggtattga taaaaccgca aatcttggaa
     4621 cctgacttta cctactagag gaacctcaca tgacggatgc aaacgaaaac caaacagata
     4681 aaaagttaga ggcaagtgga aatcccatcc ctgtccctcg tgaaaccaag gctgccgttt
     4741 ctacagacaa caaccaagtc atggattctt ctgcaacttc aaatccagcc ttgccggcga
     4801 taaaagcgcc cggtgattct tcgtctaagg cagaatcggt tccatcaggg acagagccaa
     4861 aagcgccgaa acaaccccga gtttcggatg atcgggatta tgacatagat ggttccaaca
     4921 aagacaagtc gctgcatcct gaagtcaaac cgcatgtttt tgttgttatg ccatttggta
     4981 acaaaaaggg agccgatggt catccgttcg atttcaatgc aatttacaag accttaatca
     5041 gacctgccat cgaagatgcc ggcttcgagc cttttcgagc agatgaagaa accgcctcag
     5101 gcgacatcct gaccgacatg ttccaagagt tgcttttggc agatatggtc atttgcgatc
     5161 taagcatcga caacgccaat gctttttatg aattaggaat tcgtcatgcg ttacgcaaac
     5221 gtggtgtggt tcatattcaa gccgggcgcg cttacatgcc atttgatgtg ttcaacgtac
     5281 gtactttgcc ctatcacatc acatcagaag gtctgccaga tccggcatat attcgcagtg
     5341 acattaaggc aattgcgcgc cttctcaaag atacctgggc ttcagaccga gaactagtac
     5401 atagcccaat ctacaacctg ctcacagggc ttaaggaacc aagccgcaaa agtttgcaaa
     5461 cccccttagc aactggcttt tggcgcgaat atgaggaatg gaaagacaga gtcaccattg
     5521 ctcaacgcaa taaacacatt ggtgatattt tgctcttaac tgaagaaatc agaaacccgc
     5581 tcattcgcga agatgcagtt gctgaagtag gcggcgcact tgcccggttg ggacgtaacg
     5641 agttagctct aaaacaatac cgcgaaggga ttgacgtcaa cggggaaaat cttgaatttc
     5701 gccgggaaga agcttttcac cttaaccgtg taggaagggt gaatgaagcc atcgtaaaac
     5761 ttgaaagtat tttgaa
//
LOCUS       DAOQFT010000078         5702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7121_length_5702_cov_6.954666, whole genome shotgun sequence.
ACCESSION   DAOQFT010000078 DAOQFT010000000
VERSION     DAOQFT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7121_length_5702_cov_6.954666"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..2072
                     /locus_tag="PLR93_03145"
     CDS             120..2072
                     /locus_tag="PLR93_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNH25985.1"
                     /translation="MIELNQLELEASKSLVRILPRVEQNLKRFITKDPQAWAQFTTRL
                     QKNFPALLGFYADVYSYRYDFFYHLEDLVMSMARSWFERPQDLRELDDAREQNPHWFQ
                     SNQMLGGVCYVDLFANNLDGIRKKIPYFKELGLTYIHLMPLFKAPGGENDGGYAVSSY
                     REVHPPLGTMKQLSSLARDFREAGISLVVDLVFNHTSDEHIWAERAKAGDEEYMDFYR
                     IFPSRELPNRYEQNLREIFPEEHSGAFSPFIINGKEHWVWTTFHSYQWDLNYDNPAVF
                     NRMAEEMLFLANQGVEVLRLDAVAFIWKQLGTNCENLPQAHTLIKAFNAVARITAPAL
                     IFKSEAIVHPDDVVKYISLEESQISYNPLLMALLWNSLATRKVNLLSQALAHRFKLPQ
                     GTAWVNYVRVHDDIGWTFSDEDAAILGVNGHDHRQFLNEFYRGRFPGSFARGLPFQEN
                     PITGDCRISGTCASLAGLEKALHEEGEKEVELAIRRILLIHGVIITVGGIPLIYLGDE
                     IGTLNDYTYRDDPAHERDSRWVHRPRADWERYKKRNNLHSLEGHIFHGFRSMIEMRKA
                     NDVFAGGDLEIIPTDNSHVLGFMRTHAGKRAVVFANFSETTQTLPPRIIEQYSITSLK
                     KLHGHNHVSAKKSLELDPLDFVVFGS"
     gene            2315..5053
                     /locus_tag="PLR93_03150"
     CDS             2315..5053
                     /locus_tag="PLR93_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNH25986.1"
                     /translation="METLVTPGFDPAHDVLHYGRQPLDSIFAPKSVAVVGATETVGSV
                     GRTIVWNLMSSSFGGTIYPVNPKRPSVLGIKAYPDLKSIPEKLDLIVVVTPAPTVPDI
                     IKEAVDLGVPSAIIISAGFKETGPAGVELERQVMEHARRGNMRIIGPNCLGVMSPTTG
                     LNATFASTIARRGSVGFISQSGALCTAILDWSLTENVGFSSFVSIGSMLDVDWSDLIY
                     YLGDDPHTKSIVIYMETIGNARAFLSAAREVAMTKPIIVIKPGRTEGAAKAAASHTGS
                     LTGSDEVLEVAFRRSGVLRVNSIAELFYMAEVLGKQPRPKGPRLTIITNAGGPGVLAT
                     DALISNGGELTTISPETMEEFNKLLPAAWSHNNPVDILGDASPERYAKALEIAAKDEN
                     SDGILVILTPQAMTDPTETADVLKPYAQGLGKPVLATWMGGKEVAPGEAILNKVNIPT
                     FPYPDTAARVFDYMAHYSENLHSLYETPMSMDDGGDGIDRERAAKLIEDVRKSGRTIL
                     TEYESKQLLACYGIPTVDTRIAKSKEEAVKAADAIGYPVVLKLHSETITHKTDVGGVQ
                     LNLADAKDVKKAYESIEKSVTEKAGAEHFLGVTVQPMAKVDGYELILGSSIDPQFGPV
                     LLFGLGGQLVEVFKDRALGLPPLNTTLARHMMEKTRIYTALKGVRGRNPVDLAALERL
                     LVRFSQLITENRWIQELDINPLMASPEKLIALDARVLVYGKDVTQAQLPKMAIRAYPM
                     KYVSSWTAKDNTSVTIRPILPEDEKLLVRFHADLSDRSVFLRYLQPMMFQERVVHERL
                     SRICHCDYDREIALVAEKKVDGESIIMGVVRLSKLHANSEGRLSILVADPYQGTGLGG
                     EMVRRMIDVAKQEHLEKISAILTDDNQVMGQLFKKLGFTIEATAENPKLMNALLML"
     gene            complement(5100..>5702)
                     /locus_tag="PLR93_03155"
     CDS             complement(5100..>5702)
                     /locus_tag="PLR93_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH25987.1"
                     /translation="KYFGPHAGILYGKRELLEKLVAYKVRPATNELPGKFETGTQNHE
                     GIAGILGAIEYFEWVGKEFGGEYADELKQQGYSGRRLTLKQAMSAIHAYEFELSRVTL
                     STLEAIPGIHIFGNTDPRRLDERIATFSFRIGDMNPQTIAEKLAAENIYVWNGNYYAI
                     NVSERLGVEEKGGMVRVGPVHYNTVEEINKLGDVLKKIAG"
BASE COUNT         1403 a         1490 c         1415 g         1394 t
ORIGIN      
        1 gttcaatcgc ctgccatcct tgaaaacatc gaatcctata tcgttaatcc cggtttgggc
       61 aatcgctcgg gtatgttggg cgccattgca cttgctgaac aatccttatt aagagcatca
      121 tgatcgaact taatcaactc gaacttgaag ccagtaaatc gctcgtgcgc attttgccgc
      181 gtgtagagca aaacctgaaa cggttcatca ccaaagaccc gcaggcgtgg gcgcaattca
      241 caacccgctt gcaaaagaat ttcccggcat tgttgggttt ttatgccgat gtctatagct
      301 accgttatga tttcttttat cacctcgaag accttgtgat gagcatggcg cgttcatggt
      361 tcgaacgtcc acaagatttg cgcgagctgg atgatgcgcg tgagcaaaac ccgcattggt
      421 tccaatcaaa tcagatgctc ggcggcgttt gctacgtgga cctctttgcc aacaacctcg
      481 atggcattcg caaaaagatt ccttacttca aagaacttgg tcttacctac attcatctca
      541 tgccgctctt caaagcgccc ggaggcgaga acgatggcgg ctatgccgtc agcagctatc
      601 gtgaggtaca tccgccgctc ggaacgatga agcagctttc atcgctggcg cgtgattttc
      661 gtgaagcggg catcagcctt gtcgttgacc tggtattcaa ccatacctcc gatgaacaca
      721 tctgggcaga acgcgccaaa gccggcgatg aagaatacat ggacttctac cgcatctttc
      781 ctagccgtga gttgcccaac cgctacgagc agaatctcag ggagattttt cccgaagagc
      841 attcgggcgc gttctctcca ttcatcatca acggcaaaga acattgggtg tggaccactt
      901 tccactccta tcaatgggac ttgaactatg acaacccggc ggtctttaac cgcatggcag
      961 aagagatgct cttcctcgcc aatcaaggcg tggaagtgtt gcgccttgat gcagtggctt
     1021 tcatttggaa acaactcggc accaattgcg aaaacctgcc gcaagcacat accctgatca
     1081 aggctttcaa tgcagtggca cgaatcactg cgcccgcctt gatcttcaaa tctgaagcga
     1141 ttgtgcatcc agatgatgtt gtgaaataca tctcgctcga agaaagccag atttcttaca
     1201 acccgcttct gatggcatta ttgtggaact cgcttgccac gcgaaaagta aacttgcttt
     1261 cgcaagcctt ggcacatcgc ttcaagcttc cgcaagggac ggcttgggtg aattacgtcc
     1321 gtgtgcatga tgatatcggc tggaccttct ccgatgaaga tgccgccatc ctcggcgtga
     1381 acggacatga ccatcggcaa tttcttaatg agttctatcg cgggcgcttc cccggtagtt
     1441 ttgcacgcgg cttgcccttc caagaaaatc ccatcacagg cgattgccgc attagcggaa
     1501 cttgcgcctc cttggctggt ttggaaaaag ctctccatga agaaggcgaa aaagaagtgg
     1561 aacttgccat ccgccgcatc ttgttgattc acggtgtcat catcacggtg ggtggtattc
     1621 cgctcattta cctcggtgat gagattggca ccttgaatga ttacacctat cgtgacgacc
     1681 ccgctcacga aagagacagt cgttgggtgc accgtccgcg ggcagattgg gaacgataca
     1741 agaagcgcaa caacctgcat tcattggaag gacatatctt tcatggcttc cgttccatga
     1801 tcgagatgcg caaagcgaac gatgtttttg ccggtggcga cttggaaatc atcccgaccg
     1861 ataattcaca tgtgcttggc tttatgcgca ctcatgctgg taaacgggcg gtggtctttg
     1921 ccaacttctc tgaaaccacc cagactctcc ctccgcgcat cattgagcaa tactccatca
     1981 ccagcttgaa aaaactgcac gggcataacc atgtctctgc aaagaaaagt ctggaactcg
     2041 acccgttgga ttttgtggtg tttggctcct gatgtttttt tgatggaact tgcaagaaac
     2101 tgacggaatc gttagaaagc ggatgtcttt ttaggaagac atccgctttt ttgttgggaa
     2161 aaaatcaaga ataaggaagt taatctataa gaaacgtgaa attgagcacc cgcttctagt
     2221 ggtatatatg tctttgtatc atcctgtctt caaaactgat tgttacgatg aaaaaacatc
     2281 gtaacaacgt accctttaaa gaaagaagta aattatggaa acccttgtca ctcccggatt
     2341 cgaccccgct catgatgttc tacattatgg gcgccaaccc ctagactcta tctttgcccc
     2401 aaaaagcgtt gccgttgtcg gtgctactga aacggttggc agcgtggggc ggaccattgt
     2461 ttggaactta atgagcagct cctttggcgg caccatttac cccgtcaacc cgaagcgccc
     2521 aagcgtcctt ggcatcaaag cctacccaga tttgaaatcc attcctgaaa agttagacct
     2581 tatcgttgtc gtcacaccgg cgccgactgt gcccgatatc atcaaagaag cagtggatct
     2641 tggtgtgcca agcgccatca tcatttcggc tggattcaaa gagacgggtc cggcaggggt
     2701 ggaacttgag cgccaggtga tggaacatgc ccgccgtgga aacatgcgca tcatcggacc
     2761 gaactgcctc ggtgtaatga gcccaactac tggcttgaac gcgaccttcg ccagcaccat
     2821 tgcacgacgt ggaagcgtgg gcttcatcag ccagagtggc gcgctttgta cggctatctt
     2881 ggattggagc ctcacggaaa acgtgggctt tagttccttc gtctccatcg gctccatgct
     2941 cgatgtggac tggagcgacc ttatttacta tctcggcgat gacccacaca ccaagagcat
     3001 cgtcatttat atggaaacga ttggtaatgc acgtgctttc ctttctgcgg cgcgtgaagt
     3061 tgccatgacc aaacccatca tcgtcatcaa accggggcgc accgaaggtg cagccaaagc
     3121 agcggcttct cacaccggtt cattgaccgg tagtgacgaa gtgcttgaag ttgctttccg
     3181 tcgcagtggt gtgttgcgcg taaatagcat tgccgaattg ttctacatgg cagaagtgct
     3241 cggcaaacaa ccgcgcccga aaggtccacg ccttacgatc attaccaatg ctggcggacc
     3301 cggcgtgctc gccaccgatg cactcatttc gaatggcggt gaacttacca ctatctcacc
     3361 agagacgatg gaagaattca acaaactgct tcccgccgcg tggagccata acaacccggt
     3421 cgatatcctt ggcgatgcca gccctgaacg ttacgccaaa gcacttgaga tcgcagccaa
     3481 agacgaaaac agcgacggca ttttagtcat cctcaccccg caagccatga ccgaccctac
     3541 cgaaacggca gacgtcttga agccatacgc acaaggcttg ggaaaacccg tcctcgctac
     3601 ttggatgggc ggcaaagaag tggcaccggg tgaagctatt ctcaacaaag tgaacatccc
     3661 caccttccct tatcccgata cggcggcgcg cgtcttcgat tacatggcgc attattctga
     3721 gaacctgcac agcttgtatg aaacacctat gtctatggat gatggtggcg atgggatcga
     3781 ccgtgaacgt gctgcaaaac tcattgagga tgttcgcaaa tctggtcgca ccattttgac
     3841 agaatacgaa tcaaaacaac ttttggcttg ttacggcatc ccgacggtgg atactcgcat
     3901 cgcgaagagc aaagaagaag ccgtcaaagc cgcagatgcc attggctacc cggtggtgct
     3961 caagctccat tcagagacca tcacacacaa aaccgatgtg ggcggcgttc aactaaacct
     4021 tgcagatgcc aaggatgtga agaaagccta cgaaagcatt gaaaaatctg tgaccgaaaa
     4081 agcaggcgcg gaacatttcc tcggtgtgac ggttcaaccg atggcaaagg ttgatgggta
     4141 cgaactcatt ctcggcagca gcattgaccc gcagtttggt cccgtgttgt tgttcgggtt
     4201 gggcggtcaa ttggtggaag tcttcaaaga ccgtgccttg ggattgcctc cgctcaatac
     4261 aaccctcgca cgtcacatga tggagaagac ccgcatctac accgcattaa aaggtgtgcg
     4321 cggacgcaac cccgtagacc ttgccgcctt ggaacgcttg ctcgtccgct tctcacagtt
     4381 gatcaccgag aaccgctgga tccaagaatt ggatatcaac ccgctcatgg catcacctga
     4441 aaagctcatc gctttagatg cgcgtgtgct tgtgtatggc aaagatgtga cgcaagcgca
     4501 attgcccaag atggcgatcc gtgcgtaccc gatgaagtat gtcagttcgt ggacggcgaa
     4561 agataatacc agtgtcacca tccgcccgat tctgccggaa gatgaaaagc ttctcgtgcg
     4621 cttccatgct gacctttcgg atcgcagcgt attcctgcgt tatctccagc cgatgatgtt
     4681 ccaggaacgt gttgtgcatg aacgcctctc tcgcatttgt cactgcgact atgaccgtga
     4741 gattgctttg gtggctgaaa agaaagtgga tggcgaaagc atcatcatgg gtgtggtgcg
     4801 tttgagcaaa ctacatgcca atagcgaagg tcgtttgagc attctcgttg ccgaccccta
     4861 tcaaggaacc ggcttgggcg gcgaaatggt gcgccgtatg atcgatgttg ccaaacagga
     4921 acaccttgaa aagatcagcg ccattctaac ggacgataat caggtgatgg gtcagctctt
     4981 caagaagttg ggcttcacca ttgaagcgac tgctgaaaac cccaagttga tgaacgctct
     5041 gctgatgctg taatatcttt aaataaaaac tcccagtcga atgactggga gttttttgtt
     5101 tagcctgcaa ttttctttaa cacatcgcct agtttgttga tttcttccac agtattgtag
     5161 tggacgggtc ccacacgcac catgccgcct ttttcttcca cccccaaccg ttcagagacg
     5221 ttaatcgcgt agtagttgcc attccacaca taaatatttt cggcggcgag tttctcagca
     5281 atggtctgcg gattcatgtc gccgatgcgg aaggagaagg tcgcgatacg ttcgtccaac
     5341 cggcgcggat cggtattgcc gaaaatatga attcccggaa tcgcttcgag tgtggaaagc
     5401 gtcactcggc tgagttcaaa ttcataagca tggatggcac tcatggcttg cttgagagtt
     5461 aggcgacgtc cgctatagcc ttgttgcttc aactcgtcag cgtattcacc accgaattct
     5521 ttgccaaccc attcaaagta ttcaatggca ccgagaatgc ctgcaatgcc ttcgtggttt
     5581 tgggtgccgg tctcaaattt gccgggcaat tcgttggtgg ctgggcgcac tttgtaggca
     5641 acgagttttt caagcaattc gcgcttgccg tacaaaatcc ctgcatgtgg accgaagtat
     5701 tt
//
LOCUS       DAOQFT010000079         5687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7142_length_5687_cov_6.281250, whole genome shotgun sequence.
ACCESSION   DAOQFT010000079 DAOQFT010000000
VERSION     DAOQFT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_5687_cov_6.281250"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLR93_03160"
     CDS             complement(<1..984)
                     /locus_tag="PLR93_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25988.1"
                     /translation="MINGWPETLLAGLQTINQILTAGIAITAFSLFLYSLSFNLRDRV
                     ARSFAIILLCVVIVFTAESLQDNSVTIKALDLLMRLQWFGLAFLPAAYLHLSDALLVT
                     AGRPSRGRRRFAVRGMYIISALFLVLLAFGYLLGPIVPDGKPAPYLQRTAWTEVFTIF
                     YVTSMVWAGVNFYRAYTLMLTRSGRRRMIYLMAGATAPALGSYPYLLFGYSLASQHQF
                     LFWTAATVINALVGALVIVMAYAVAFFGVSWPDRVIKSRLFKWIMRGPVVASATLALM
                     TVVRRGGELLGTPYSAFVPFTVVASILLMEHAITFAAPLWERGLFFGRDRNE"
     gene            complement(1033..1572)
                     /locus_tag="PLR93_03165"
     CDS             complement(1033..1572)
                     /locus_tag="PLR93_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH25989.1"
                     /translation="MSELQIRPTVATDLTRLMGIDHSMTSEAVWQLEVRRSTGQVAVT
                     FREVRLPREIQVPYPHNHFALADDWVKRSMMYTAIKDAEPVGYISLLERGTASTVWVL
                     DLAIDAKQRRRGVAAALLASAQAWAESRSHRRLILEVQSKNVPAIRLAQKSGFEFCGY
                     NDQYYLNKDVALYFAKVLK"
     gene            complement(1589..2266)
                     /locus_tag="PLR93_03170"
     CDS             complement(1589..2266)
                     /locus_tag="PLR93_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH25990.1"
                     /translation="MVDLFKFLAEYEVLIYIVLAIGALFSFRWLFRSWREWQVAVFSL
                     EREFSARRFGRSALVSAMIVIFFCVEFFMASFIIPGLPADFFVATPTLDLISTPTGTL
                     SPELMTQFANQPQVAQPNAAGCTPGQIELTFPEAGTEIKGIIELTGTVDIPNFGFYKY
                     EIAPSGSDTWATIAAGRTVVKASSLGRWDTTALTPGDYQLRLVVIDNEGNSLTPCVVP
                     VQVIPNQ"
     gene            complement(2291..3127)
                     /locus_tag="PLR93_03175"
     CDS             complement(2291..3127)
                     /locus_tag="PLR93_03175"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNH25991.1"
                     /translation="MQKFISLAQIDEIVNVIKKKISIQPVIGMILGSGLNGLADSVQN
                     PVHIPYSDLPNFPTSTVHGHVGRFVIGELEGKPVIVMQGRIHYYEGYTMGEVTLPVRV
                     MHRLGVHSLFVTNAAGGVNENFVPGDVMLITDQLNLMGMSGLNPLMGPNLDEIGPRFP
                     DMSQPYDREYCDLARKVAKQSNLTLREGVYAGLSGPSFESPADLRFLRLAGADAVGMS
                     TVPEVIIARHGGMRVLGLSGVSNKANLDGSTITTHEEVIEAGRVITPKIETIVRGVLR
                     GI"
     gene            3359..>5687
                     /locus_tag="PLR93_03180"
     CDS             3359..>5687
                     /locus_tag="PLR93_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH25992.1"
                     /translation="MALIPSHFGMTHQILSIIPLGVLSFSCFITALAIQIHCVWGLIH
                     KRETLIGIITITLLFIVSFFLIQSGQYISAAAMILAGIYQTYIILPIDKKRKQVDFTN
                     LSIATITFATGIILTVTSSQDDAKLLLATSFLILAFVAGTFIVIPSFKYGQWLNKTQV
                     IPWLILVFFYILPTGEGNLVVPAAVVGTLLFSRFIPWDSLSMPANDILSRRVIVIAFV
                     VEIANLLFLGALLFAIEPEFKNNYSAIFITRGLTLAFFILSSILIYIGVMTVIMTTNG
                     LTQELNRIGFEDEENGELLEDSESPWTSRLEKYIQPYIMTSEGIRGRIQADKINALTR
                     QSAFDKKRNAQLTLLLELSQQLENQLDPPVSAQLAVNTLERALNCSLVLLFIHEPEKK
                     EFMLLAEAGRQTHLVPKEYRQDASVGVLGRALRQRKTQVVNNIQVDPDYIPFKNENNL
                     SAAVIPLIYNGHIHGAIALSNEKMNTLGSLEIGMAETVAGELVRAWERSGYHERLKEL
                     IQTGSQLSSVPDPETAVQEVALISKEIVQAKFTYIYIQLGQERNFIQHASAGQAPQLL
                     TSLREASISAELINLALHSSQPFRIRDIRRYDKTSKLVLDNNSLRSLLVIPIRWHYVN
                     IGAIFAFGKQKEVVFTENDESLAELISIQAGGAFESTWLQQELRASLRITSLLYRLSN
                     QIIQSENIEDAASDIAQTAHKLAKNLVTGIVLFNTTGEVIAEVLVNDDGKAVNPEHPM
                     SLIQDAMKSGQMIYVSPDESTIRTCLPIQSPIRGYG"
BASE COUNT         1643 a         1398 c         1251 g         1395 t
ORIGIN      
        1 ttcattgcgg tcacgcccaa agaacaagcc ccgttcccaa agcggagcag caaaggtgat
       61 ggcatgttcc attaaaagaa tagatgctac aaccgtaaaa gggacaaagg cactataagg
      121 agttccgagc agttccccac cacgccgtac gacagtcatc aatgccagtg tagcagaggc
      181 aacgacaggt ccacgcatga tccacttaaa gaggcggctc ttaatcacac ggtctggcca
      241 tgaaacgcca aagaacgcca ctgcatacgc catcacgatc accaaagcac caaccaaagc
      301 gttgattact gttgcagcgg tccaaaagag gaactgatgt tgtgatgcga gagaatatcc
      361 aaacaaaaga tacgggtatg agcccaaagc cggggcggtt gcacccgcca tcaggtagat
      421 catgcggcgt ctgcccgaac gggttagcat caatgtataa gcacggtaaa aattcacacc
      481 cgcccagacc atcgaagtca cataaaagat cgtgaaaacc tctgtccacg ctgtgcgttg
      541 aaggtaagga gcaggtttcc catctggcac gataggtccg agtaaatatc cgaatgcgag
      601 caaaacgagg aaaagcgcag aaatgatgta catgccgcgc acagcaaaac gacgccttcc
      661 acgagaaggg cgcccagctg taacaagcaa agcatctgaa agatgcaagt aagccgccgg
      721 caaaaacgcc agcccaaacc attgcaaacg catcaacagg tcaagcgcct taatagttac
      781 tgaattatct tgtagagact ctgcggtaaa aacgatcacc acacacaaca aaatgatcgc
      841 aaatgaacgt gccaccctgt ctcgcaggtt aaatgaaagc gaatagagaa acaacgagaa
      901 tgccgtaata gcgatccccg ccgtcagaat ctgattgatc gtttgaagcc ccgccagtag
      961 tgtttctggc caaccgttaa tcatgcaacc cttccaataa aaatcgtgca acgggcggta
     1021 aaaccgctcg ccttacttta ataccttcgc aaagtatagt gctacgtcct tgttaaggta
     1081 atactggtca ttatacccgc agaactcaaa tccagatttc tgcgccagcc ggatcgctgg
     1141 gacatttttt gactgcactt cgagaatcag gcgacgatgc gatcgcgact ccgcccatgc
     1201 ctgagcagac gccaacaaag ctgcagccac gccgcggcga cgttgcttcg catcaattgc
     1261 cagatccagc acccacacgg ttgaagcagt cccccgctcc aacaagctaa tatatccgac
     1321 cggctcggca tccttgatcg ctgtatacat catggagcgc tttacccaat catctgccaa
     1381 ggcaaaatga ttatgcgggt atggcacctg aatttcacgc ggtagacgaa cctcacgaaa
     1441 tgtaactgcc acctgccccg tactacgacg aacttccaac tgccaaactg cttcgctggt
     1501 catggaatgg tcaattccca tcaaacgggt tagatcagta gcaacagttg ggcgaatttg
     1561 taactcagac atgaattgaa attacctttt attgatttgg tataacctgc acaggcacga
     1621 cacaaggtgt aagcgaatta ccttcattat cgataacgac caatctcaat tgatagtcgc
     1681 caggggtgag cgctgtggtg tcccaacgcc caagagaact tgctttcaca acggtacgtc
     1741 ctgccgcaat cgtcgcccac gtgtcactgc cacttggagc gatctcatat ttataaaatc
     1801 caaaattggg tatgtcgaca gtacctgtca gttcgatgat gcctttaatt tcagtaccag
     1861 cctcagggaa tgtcaactcg atttgcccag gggtacaccc ggcagcattg ggttgcgcca
     1921 cttgcggctg atttgcaaat tgggtcatca gttcgggcga aagcgtaccg gttggtgtgg
     1981 aaatgaggtc cagagtcggt gtagcaacaa agaaatcagc ggggagacct gggatgatga
     2041 acgatgccat gaagaactct acacagaaaa aaatcacgat catcgctgag accaaagcag
     2101 aacgcccaaa acgccgagct gaaaactcgc gttccaaact aaagacagcc acctgccact
     2161 cacgccatga acgaaaaagc cagcgaaagg aaaataatgc tccaatcgcc aagacaatat
     2221 aaatcaatac ctcgtattca gcgaggaact taaagagatc taccatctaa caaccttgcc
     2281 aagttcgcgt ttatattccg cgaagaacgc cccgtacgat ggtttcgatc ttgggtgtga
     2341 tcacacgccc agcttcaatc acctcttcgt gagttgtaat agtggaccca tctaaattag
     2401 ctttgttact tacgcctgaa agccctaaga cgcgcattcc gccatgccga gcaataatca
     2461 cttctggtac tgtggacatt cctacagcat ccgcaccggc aaggcgtaag aaccgcaggt
     2521 ctgctggtga ttcaaaggaa ggtccactca aaccagcgta gaccccttca cgcaaggtta
     2581 ggttactttg ctttgccact ttacgggcaa ggtcacaata ttcacggtca taaggctggc
     2641 tcatatctgg gaagcgcggt ccaatctcgt cgaggttggg tcccatcaag gggttcaaac
     2701 cagacatccc catcaggttt aactggtcag tgatcaacat cacatcacct ggtacgaaat
     2761 tctcgttcac accgccagcc gcattggtta caaataaact atgaactcca agtcgatgca
     2821 tgacgcgcac aggcaacgtt acttcgccca tggtgtagcc ttcataataa tgaattcgtc
     2881 cttgcatgac gataacgggt ttgccttcta gttcaccgat cacaaagcga ccaacatgtc
     2941 cgtggacggt tgaggtgggg aagtttggta ggtcactgta tggaatgtga accggattct
     3001 ggacagaatc agccagcccg ttaagccctg agccgagaat catcccgatc actggctgaa
     3061 tggaaatctt cttttttatg acattaacga tctcgtcgat ttgagcaaga gagataaatt
     3121 tctgcattta catttacctt atatcgtcag gattacaaac ggctcagccc cataaagacc
     3181 agtttgcgaa atgattataa cagaaatgac cctgcgggct tttcagccct aaaatgcaca
     3241 attgaagtga actagataaa atttgtttct tatgctatcc tctagcgaca tgaagcgccc
     3301 taacgagaaa aatttcaaga tatttgtttc cttactgcta gaaggcgttg gggcaggaat
     3361 ggctctcata ccaagtcatt ttgggatgac ccaccaaatc ctctcaatca tccctttggg
     3421 agttctaagt ttttcctgct ttattactgc tcttgcaatt caaattcact gtgtgtgggg
     3481 gctgatccat aaacgtgaaa ctctgattgg cataattact atcacgctgc tttttattgt
     3541 cagctttttt cttatccaaa gcggacaata catttcagca gcagccatga tccttgcggg
     3601 catttatcag acctatatca ttttacccat cgataaaaag cgaaaacagg ttgatttcac
     3661 aaacctatcc attgcgacaa taacatttgc aacaggcatc attttaactg tcacaagttc
     3721 tcaagatgat gcaaagcttt tgttggcgac ttcgttcttg atcttagctt ttgtagcagg
     3781 gacgtttatc gttatcccgt cgttcaaata tgggcaatgg ctgaataaaa cgcaggtcat
     3841 tccctggctc atccttgtgt tcttttacat ccttccaaca ggcgaaggga atttggtcgt
     3901 gccggcagct gtggtaggta ccttattgtt ttctcggttt atcccttggg atagcctcag
     3961 tatgcctgcc aatgacattt taagtcgacg cgtgatcgtg atcgccttcg tagtagaaat
     4021 cgccaatttg ttatttttgg gtgcgttact atttgcaatt gagcctgaat ttaagaataa
     4081 ctattctgcc atctttatca cacgcggctt gactctggca ttttttatct tgtcctcgat
     4141 tttgatctac attggggtaa tgaccgtgat catgaccacc aatggattaa ctcaagagtt
     4201 gaacagaatt ggttttgaag atgaagaaaa cggcgaactc cttgaagact cagaaagccc
     4261 atggaccagt cgcctagaaa aatacatcca gccatatatt atgacttcag agggcatccg
     4321 tgggcgcatt caagcggaca agatcaatgc ccttactcgt caatctgcat ttgataaaaa
     4381 acgaaatgca caactaactt tgttattgga attaagccaa cagctcgaaa atcagctcga
     4441 cccgcctgtg tcagcacagc ttgcggtcaa cacgcttgaa cgagctttaa attgtagttt
     4501 ggtcttgttg tttatacacg agcctgaaaa aaaggaattc atgcttttgg cagaagcggg
     4561 cagacaaaca catctcgttc ccaaagaata tcggcaagat gcatcggtag gagtattggg
     4621 tagagcatta cgccaaagaa aaacacaggt tgttaataat attcaagtgg atccagatta
     4681 cattccgttt aagaatgaga ataacttatc agcagcagtt atccctctta tctataacgg
     4741 tcatatccac ggggcaatag ccttaagcaa tgaaaaaatg aacacgcttg gcagtcttga
     4801 gatcggcatg gcagaaaccg ttgctggcga attagtacga gcgtgggagc gttctgggta
     4861 tcatgaacgt ctcaaagagc ttattcaaac tggtagtcag ctttcttccg tcccagaccc
     4921 agaaacagcc gttcaagaag ttgccttgat ctcaaaagag atcgtacagg caaagtttac
     4981 gtatatctac attcaacttg ggcaagaacg aaatttcatt caacatgcct cagcagggca
     5041 agcgccacaa ttgctgacct cgcttcgaga ggcaagtatt tctgccgagt tgatcaattt
     5101 agccttgcat tcttctcagc ctttcagaat cagagatatc cgccgttacg acaagacctc
     5161 taaactggtc ttagacaaca atagcttacg tagtttattg gtaattccca tacgctggca
     5221 ttacgtcaat atcggtgcaa tttttgcctt tggcaagcaa aaagaagtgg tctttacaga
     5281 aaatgacgag tccctggcag aattaatctc catccaagca ggtggcgcct tcgaaagtac
     5341 ttggctacaa caggaattac gtgcatcact acgtatcacc tccttgctct atcgcttgag
     5401 caatcagatc atccaatcag aaaacataga agatgccgct tcagatattg cgcagaccgc
     5461 tcataaatta gccaaaaact tggtgacggg tatcgtgctc ttcaatacaa caggagaggt
     5521 cattgcagag gtattggtca atgacgatgg caaggctgtc aacccagaac atcccatgag
     5581 tctgattcaa gatgcgatga aatcaggtca gatgatctat gtctcacctg atgaatcgac
     5641 gatccgcacc tgcttgccca ttcaatctcc cattcgtgga tatggtg
//
LOCUS       DAOQFT010000080         5687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7143_length_5687_cov_5.243963, whole genome shotgun sequence.
ACCESSION   DAOQFT010000080 DAOQFT010000000
VERSION     DAOQFT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7143_length_5687_cov_5.243963"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1248)
                     /locus_tag="PLR93_03185"
     CDS             complement(205..1248)
                     /locus_tag="PLR93_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNH25993.1"
                     /translation="MSLPKIDEKYFTTFLVDLLNIPSPTGFASAAIDFVEKELSKYKQ
                     LELSRTRKGALVAKWKVESDLPPVALTAHVDTLGAVVKEIKGNGRLRLSRIGGIQWPT
                     IETEGVWVFTSKGEKIRGSVLIDVASGHIHSGPDLPRDEKHLEVRLDVKTTSEAETRA
                     LGINIGDCVAFDTRTEVTNGFIRSRFLDDKACVANLVAAIKSMVESGQSPARTVYFHI
                     SNYEEVGHGAAAGIPSEVAELITVDMAVVGPGQESDEFHTTLCIKDSGGPYHEGLNKK
                     MRGIAEKHGIPYVTDVYPFYGSDGEAFWRAGGDVAMSLIGPGIDASHNYERAHMDGLN
                     ATTNWIMAYLLEH"
     gene            complement(1251..1670)
                     /locus_tag="PLR93_03190"
     CDS             complement(1251..1670)
                     /locus_tag="PLR93_03190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNH25994.1"
                     /translation="MTDYNFFHPTEVRYGDLDPQGHVNNAKYLTYFEQARVYYFMKLG
                     LFSKDQSFMEIGVIVADIHITYHAPTHYGDNIKAGAKVTKIGNKSMTVEQCIVNADTG
                     KVMASGEVVLVTFDYEGLKTIPVPDDWKKKLSEYEGL"
     gene            complement(1812..2519)
                     /locus_tag="PLR93_03195"
     CDS             complement(1812..2519)
                     /locus_tag="PLR93_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH25995.1"
                     /translation="MLRMVELKKDDTRNLLSDIRLFEGLDPAQLDWVAHRAHRRVFPT
                     GKNVMLIEQPGEAVYVILHGTVKVYTEQGGRDAILSILGSGDLIGEMSLIDSVGRSAS
                     VVTLEDSLMLWMDKTTFFYMLDNFPPVARNLVKILSARVRLSDQMIQSLATLDVNGRV
                     ARQLLAFAERYGRDMDGGVKIRIALTQSDIADLVGASRKRVNQAMVFFKEQGLATTDT
                     DGHIIIQDKAGLAKYCD"
     gene            complement(2503..>3161)
                     /locus_tag="PLR93_03200"
     CDS             complement(2503..>3161)
                     /locus_tag="PLR93_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH25996.1"
                     /translation="GTAETVKHVLEHNQSSDTFPISLRDMASNKDIQSLRETQVIVWD
                     EDGVRVQESSPSPSPEAVVIRIHKSYAHPGSVYHYRIEYQGKSIVYATDTEGYVGTDR
                     KLVNFTRNADVLIHDAQYSDDHYYGCLAGFPSTQGYGHSTATMAGQVAASAETGQLIL
                     FHHDPAYADDWVAAQERAAKNIFPDSLAAFEGMTLALTPQPLSQRARGVKEVKYVTHG
                     "
     assembly_gap    3162..3261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3262..3538
                     /locus_tag="PLR93_03205"
     CDS             <3262..3538
                     /locus_tag="PLR93_03205"
                     /inference="COORDINATES: protein motif:HMM:NF017879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNH25997.1"
                     /translation="GFSWSIVYGRKGIILCMLPDLQMNDHLRLRKPHPCGSYEWTVVR
                     LGADIGLECKGCQHRVMLTRRELTKRMKTNLSQQEREKQRDSEENNT"
     gene            3562..4884
                     /locus_tag="PLR93_03210"
     CDS             3562..4884
                     /locus_tag="PLR93_03210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH25998.1"
                     /translation="MDPQNTDLPIGSLRIGLFTDTYAPQVNGVSVSLQMISEGLKKRG
                     HQVTIFAPKFPGYKDNEPNVMRLPSLKYLNNPPIYVAVLGTPRSTWKLTRKHFDVLHA
                     HSPASVGLLAYLTASTKRLPLIYTYHTSITDYTHYIKFIGNTGIIKYAASWFSKASTD
                     LGDQIIVPSPKFYRLLLNQKVKQPISIIPNGIDLSMFKVAKNPGALRKRLGLGADVPI
                     MLTVGRMDPEKRLDFIVEAFDLIAERVPNAHLVFAGDGGARKHVEEKANSTRAKDRIH
                     FLGMVNRADLPDILHDANVFLSASTTEVHPISVIEAIASGLPFVAVQDEAFEGMLEEG
                     QNGYTVPLDVKKYADVLADLLPDRERLQRFSAHSRELSEKYSIEAQVKALEKLYMEAI
                     LQNWRGTLFKRIIPRQLNEIPQRISKEINEFPQKITKEINKILPGKKD"
     gene            4983..5456
                     /gene="tsaE"
                     /locus_tag="PLR93_03215"
     CDS             4983..5456
                     /gene="tsaE"
                     /locus_tag="PLR93_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNH25999.1"
                     /translation="MLEFFSRSPEQTRRIGMRLGNLLQAGDIICLQGDLGAGKTTFTQ
                     GLAQGWGSLDSVSSPTFILVNQYRRADSAQLFHLDAYRLETVPEAEELDLDSMLAEGA
                     LIIEWPERLGDLIPAEHLHIRLEHLSEEHRQMNFHAHGKRYDHLLDSIRQSVVGG"
     gene            5458..>5687
                     /gene="tsaB"
                     /locus_tag="PLR93_03220"
     CDS             5458..>5687
                     /gene="tsaB"
                     /locus_tag="PLR93_03220"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNH26000.1"
                     /translation="MLLAVDTSTALVGLALYDGDQVLGEMTWTTKQHHTTELSPALSG
                     LLKRCGVTMASVNALGVAIGPGSFTSLRVGLS"
BASE COUNT         1359 a         1483 c         1340 g         1405 t
ORIGIN      
        1 tcgcggatga agaacacgat cacattgctc ttaccgcgga aatttcccag cgaaatagac
       61 tgcccattgg tggctgtcaa cttgaagtct ggtgcgagcg agccgatctt ggcttcaata
      121 gagtcgaggg tcatatccga ttcctatttt gatttctact gataaagatg gtatctaacg
      181 ccaactcttt acgttagcgt ttggttaatg ttccaacaaa tacgccatga tccagttcgt
      241 ggtcgcgttg agcccatcca tgtgagcgcg ttcgtagttg tgagatgcgt cgatgcccgg
      301 gccaatcaac gacatggcaa catcaccgcc tgcgcgccag aaggcttcac catccgagcc
      361 gtaaaatgga tacacatcgg tcacatacgg aatgccatgt ttctcggcaa tgccgcgcat
      421 ttttttattc aagccttcgt gatacggtcc gccgctgtct ttgatgcaca aagtagtatg
      481 aaactcgtcc gactcctgtc cgggtcccac cactgccata tccactgtga tgagctccgc
      541 cacttcactt gggatgcccg ccgccgcgcc gtgccccacc tcttcatagt ttgagatgtg
      601 aaagtacacg gttcgtgccg gactttgacc tgattccacc atcgacttga tcgccgcgac
      661 aagattcgcc acacaagcct tgtcatcgag aaagcgcgag cggatgaatc cattggtcac
      721 ttcggtacgc gtatcgaacg ccacacaatc gccgatgttg attcccaatg cgcgagtctc
      781 ggcttcggat gtcgtcttta catcgaggcg cacttcgaga tgcttttcat cgcgcggcaa
      841 atccggtccg ctgtggatgt gaccagacgc cacgtcgatc agcaccgagc cgcgaatctt
      901 ttcgcctttg gatgtgaaca cccacacgcc ttcggtttca atggtcggcc actgaatccc
      961 accaatgcga ctcaaacgta aacgcccatt acctttgatc tctttgacaa ccgcgccaag
     1021 cgtgtccaca tgggcggtca acgcaacggg aggtaaatca ctttctactt tccacttggc
     1081 aactaatgct cccttacggg ttcggctcaa ttccaactgt ttgtatttgg atagctcttt
     1141 ttcaacaaaa tcaatcgccg cgctggcaaa cccagtcggc gaagggatgt tgagcaggtc
     1201 aacaaggaaa gttgtgaaat atttttcgtc aatttttggg agagacatgt ttacaaccct
     1261 tcatattctg ataacttctt cttccaatca tccggcacag gaatggtctt caaaccttcg
     1321 taatcgaaag tgaccagcac cacttcgccg cttgccatca ctttgcccgt gtctgcattc
     1381 acaatgcact gctctaccgt catagacttg ttgccgatct tggtgacctt tgcgcctgct
     1441 ttgatattgt caccgtaatg cgtgggggcg tgataggtaa tgtgaatatc tgccacgatg
     1501 acgccaattt ccataaagga ttggtctttg ctgaacaaac caagcttcat gaaatagtag
     1561 acacgcgcct gctcgaaata cgtcaggtat tttgcgttgt tgacatgtcc ctgcgggtcc
     1621 agatctccgt agcggacttc ggttgggtgg aagaagttgt aatcggtcat gaagcgatta
     1681 tagtttggta attctgagtt gggtagtttt tagcctgttc gtcgaatggt cagatggttg
     1741 catggctaaa aaatagccgc agagcggaac gctccacggc tatttgacta agagactatt
     1801 cgactactca actaatcaca atacttcgcc aaccctgctt tatcttgaat gatgatgtgc
     1861 ccatctgtat cggtggtggc gagtccctgc tctttgaaga ataccatggc ttggttcacc
     1921 cgtttgcgcg aggcgcccac caggtcagca atgtcacttt gggtgagcgc aatgcggatc
     1981 ttcacgccgc catccatatc gcgcccgtag cgttcagcga acgccaacag ttgacgcgcc
     2041 acccgtccgt tcacatctaa tgtggcaagc gattggatca tctggtcaga aaggcgcact
     2101 cgtgccgaaa ggattttcac caaattgcgc gctacgggcg gaaagttatc gagcatgtaa
     2161 aagaacgtgg ttttatccat ccacaacatg agcgagtctt cgagggtcac cacactggca
     2221 gagcgcccta cgctgtcgat caaactcatt tcgccgatta gatctccgct gcccaaaatt
     2281 gacaaaatcg catcccgtcc gccttgttcg gtgtagacct tcacggtccc atgcaggatg
     2341 acatacaccg cttcgcctgg ctgttcgatc agcatcacat tcttgccagt cggaaacaca
     2401 cgccgatgtg cgcggtgagc cacccagtct aattgtgcgg ggtccagccc ttcaaacaaa
     2461 cgaatgtctg aaagcagatt gcgggtgtcg tctttcttga gttcaaccat gcgtaacata
     2521 ttttacttcc tttactcccc tcgccctttg ggagaggggc tggggggtga gggcaagtgt
     2581 cattccctca aacgccgcta gtgaatcagg aaaaatattt ttcgcagcac gttcttgcgc
     2641 cgccacccaa tcgtcagcat aggctgggtc gtgatggaac aggatcaact gtcccgtttc
     2701 ggcagaggcg gcaacttgcc ctgccatcgt tgcggtcgag tgaccatatc cctgcgtcga
     2761 aggaaatcca gcgaggcatc cgtaataatg atcgtctgaa tattgcgcat catgaatgag
     2821 cacgtcggca ttgcgcgtga agttcaccaa cttgcggtct gtgcctacat atccttcggt
     2881 gtcagtggcg tacacaatgg acttgccttg atattcgatg cgatagtgat acacgctgcc
     2941 gggatgcgca tacgatttat gaatgcggat gaccaccgcc tcaggactcg gacttgggct
     3001 tgattcttgg accctaactc catcctcgtc ccaaacaatg acctgcgtct cacgcagcga
     3061 ttgaatatct ttgttcgaag ccatgtcacg caatgagatc gggaatgtgt ctgaagactg
     3121 attgtgctcc agcacatgtt tcaccgtctc agccgtgccg cnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn ncggtttttc atggtctatc gtctatggtc gtaaaggtat
     3301 aatcctttgt atgcttccag acctgcaaat gaacgatcac ctacgtttgc gcaaaccaca
     3361 tccctgcggc tcttacgaat ggaccgtggt gcggctcggc gcagatatcg gtttggaatg
     3421 caaaggctgc cagcatcgtg tcatgctcac ccgccgcgaa ctgacaaaac gcatgaaaac
     3481 gaatttgtct caacaggaac gggaaaaaca aagagactca gaagaaaata acacgtagat
     3541 aaatacaaac aaggagcata catggaccca cagaatacag atctccccat tggttcactt
     3601 cgcattggct tatttaccga tacctacgct ccacaagtaa atggtgtttc ggtctcgttg
     3661 cagatgatct cagaaggctt gaagaagcgc ggacatcaag tcaccatctt tgcgccaaaa
     3721 ttccccggct ataaagacaa tgaaccgaat gtgatgcgcc tgccttcgct gaaatatttg
     3781 aacaatccgc ctatttacgt ggcggtattg ggaactccgc gctctacgtg gaagttaacg
     3841 cgcaagcatt tcgacgtgct gcatgctcac agcccggcaa gtgtggggct actggcgtat
     3901 ctcacagctt ccacaaagcg actgccattg atctacacgt atcacacgtc tattactgat
     3961 tacactcatt acatcaagtt catcgggaat acaggcatta tcaaatatgc ggcatcatgg
     4021 ttcagtaagg catctacaga cttgggggat cagatcatcg tcccttctcc gaagttctat
     4081 cgtttacttt tgaatcaaaa agtgaaacaa cctatcagta ttattccaaa tggaattgac
     4141 ctgagcatgt ttaaggtcgc gaaaaacccc ggcgcattga gaaagagact ggggttaggt
     4201 gccgatgtgc cgattatgct caccgttggg cgcatggacc cggagaagcg tcttgatttc
     4261 atcgttgaag cttttgacct gattgcagaa cgtgtgccaa acgctcacct cgtctttgct
     4321 ggcgatggcg gcgcccgcaa gcatgtggaa gaaaaagcaa attccacccg tgccaaagac
     4381 cgtatccatt tccttggtat ggtcaaccgt gctgatctgc ccgatatttt gcacgatgcc
     4441 aatgtgttcc tctcggcatc caccaccgaa gttcacccca tctcggtcat tgaagcaatt
     4501 gcctctggtt tgccattcgt cgccgtgcag gacgaagcct ttgaaggcat gctcgaagaa
     4561 ggtcaaaacg gctatacggt tccgctcgat gtgaagaaat atgccgatgt acttgccgac
     4621 ttgctcccag accgtgaacg gttacagcgt ttcagcgcac actctcgcga actgagcgaa
     4681 aagtattcca tcgaagcaca ggtcaaagct ttggaaaaac tgtacatgga agccatcctt
     4741 caaaattggc gcggaactct cttcaaacgc ataattccca gacagttaaa cgaaatccct
     4801 caaaggattt cgaaagagat caacgaattt ccgcaaaaga tcacgaagga aattaacaag
     4861 atcctgccag ggaaaaaaga ctaaataatt acgggttacg tatccacaat gcgtaacccg
     4921 taatttgtaa ctggcaaggc ggggggtata attccaaccc gatgtccatc cttgatgccc
     4981 acatgctgga atttttcagc agaagccccg aacaaacacg acgaattggt atgcgccttg
     5041 gcaatttgct tcaggcgggc gatattattt gtttacaagg tgaccttggt gcgggcaaga
     5101 ccaccttcac ccaggggttg gcgcaaggtt ggggctcgct cgattctgtt tcaagcccca
     5161 cgtttattct ggtcaatcaa tatcgacgtg ccgacagtgc ccaacttttt cacctcgatg
     5221 cgtatcggct cgaaaccgtg cccgaggctg aggaactcga cctcgactct atgctcgctg
     5281 aaggggcact catcatcgag tggcccgaac gcttgggcga tcttatcccc gccgagcatt
     5341 tgcacatccg gcttgaacat ctttccgaag aacaccgcca aatgaatttc cacgcgcacg
     5401 gcaaacgcta cgatcatctc ttagactcca ttcgtcaatc tgtggtggga ggttaatatg
     5461 cttctagcag tagatacctc tacagcattg gttggattgg ctttatatga cggtgaccaa
     5521 gtcctcggcg agatgacgtg gacaaccaaa cagcatcaca ctaccgagct ctccccggct
     5581 ctttctggac ttttgaaacg ctgcggcgtg accatggctt ctgtcaacgc tttgggcgtc
     5641 gctatcggac ctgggtcatt cacgagtttg agagttgggt tgtcttt
//
LOCUS       DAOQFT010000081         5678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7154_length_5678_cov_5.683977, whole genome shotgun sequence.
ACCESSION   DAOQFT010000081 DAOQFT010000000
VERSION     DAOQFT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7154_length_5678_cov_5.683977"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1625
                     /locus_tag="PLR93_03225"
     CDS             261..1625
                     /locus_tag="PLR93_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNH26001.1"
                     /translation="MFKSLSFLKDRTFLNEMLVIALPISFQQLINASLNMIDVIMVGQ
                     LGETSIAALGLSNQVFFVFILLLFGLTSGMAIFTAQFWGKQEVDPIRKVLGMSLAITS
                     AIALLFTLAATLIPRTVLGLYTHDKEVIEIGASYLSIVGFSYIPVAIATSYIATLRSI
                     QLVKLTVIATISALIFKTILGYILIFGVFGLPALGVRGAAIGTASGWTLELILLIILV
                     YAQKTPLAANPLTFFSFDFSFFGRVLKTTLPALANEMFWSLGITTYNAIYARIGTDSI
                     AAINVNATIEELGFVVFMGLGNACAVMVGNRIGAGKKEEAFETVRRVVILALLSAWTV
                     GLIVLFLRDIVVNLYDLSPSGEYNVRMLMLMMACTLWIRMFNFSTFIGALRAGGDTRF
                     ALLMELCSIWLIGVPAAYIGAFVLHLPVYFVYLMVALEEVAKAFVSGWRVYSRKWIHD
                     LVNV"
     gene            complement(1662..2318)
                     /locus_tag="PLR93_03230"
     CDS             complement(1662..2318)
                     /locus_tag="PLR93_03230"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH26002.1"
                     /translation="MINQIEQNILNLLQHLYDELGWFGVTIIMIVENSTGIAPSEVVL
                     ALAGWMLIDAHSLPFSTVFLGGLFAAFGSVMGASILYWAARLGGRPVVERVTKFFRID
                     LRHMQRVERLAEKHGTRFIFFGRLIPGIRMLVAIPAGLTRMPYLTFAIATFLGAYLWC
                     TVFISVGYFLGHEWHLISNIVKQIGPYAFGIAVFFTGIFFIWRRWQRQKLETVKLEIE
                     "
     gene            complement(2401..2775)
                     /locus_tag="PLR93_03235"
     CDS             complement(2401..2775)
                     /locus_tag="PLR93_03235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNH26003.1"
                     /translation="MKNIEQFKKQQYINLETFRKNGGGVKTPVWFAQDGETLRIWTQA
                     DAGKTKRIRRDGKVRIAPSTASGEPIGEWIDANATVLNTQDEINYTKDLFRKKYGVMF
                     NVFGFLGKVRGSSFVTIKVNLN"
     gene            complement(2772..3566)
                     /locus_tag="PLR93_03240"
     CDS             complement(2772..3566)
                     /locus_tag="PLR93_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNH26004.1"
                     /translation="MTYFGFLLRFLVIPILIFLVITWWDNKNNRPTFGFNNGKAVWVA
                     ILIHIVLAVVYTTPWDNYLVATGVWYYNPNLVTGIVIGYVPIEEYTFFVLETILAGLW
                     WWFLARRIPEPTNTFKPNKTGRIISFSVLFIFWVIFTYLFFFGDVELTYLSIILFWAF
                     PAIFPQMLYGADILWHYRKLVFITIFVPGLYLSLMDIIALTDTTWSISKEQTTGILFF
                     NILPLEEVLFFFITNVLIGFGMTLLLSKLGTQRFEDWKSNNYKGLP"
     gene            complement(3632..4897)
                     /locus_tag="PLR93_03245"
     CDS             complement(3632..4897)
                     /locus_tag="PLR93_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNH26005.1"
                     /translation="MAQQLSPETGRATLKALFHERSPLGPLKVMAKHVGRFFQIPIPG
                     FSPYVVFGAEAARKVLITDRKKLLWRNTDPVTDLLRRGVLVTDGEEHDHYRSLMETPF
                     HPSQLSKYSEMMIQQVDRVISTWQDGQIVDMLVEGRKIALLIIMQTLFNKDAWNDIPK
                     IWKPILKTIQYVSPGMWILWRKIPRLGYGKSLKVIDDYMYAIIDERRKEERKTNDFLQ
                     HLIDAGLSDDIIRDQMLTMLIAGHDTSTALFAWTFALLGQHPEIHANVVQEVDRAEGK
                     YQLLDNVIKEALRLYPPIHIGNRRVAEEMEFAEGKVPAGERMFYSIYLTHRDPEVWEN
                     AEQFCPERFAHGRKTPPMSYIPFGGGPRACIGAAFGQAEARIVLAHLLKNFTFEFTGH
                     TIHAHMGATLEPRPGVKMKVKRRTVASVQ"
     gene            complement(4900..>5678)
                     /locus_tag="PLR93_03250"
     CDS             complement(4900..>5678)
                     /locus_tag="PLR93_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH26006.1"
                     /translation="GTGKPVFILLHGFGASLFSWHEVTTPLAEYGTVIAYDRPAFGLT
                     ERPLEWEGESPYSQDSQVELVIGLMDALGIEKATLVGNSAGGTVSMLTALKYPERIES
                     LILVDPAVYAGGGAPAWIRPLLGTPQMRHVGPLISRQLQAQGVEFIKTAWHDPSKIPQ
                     STLEGYQKPLQVENWDKALWELTLASRESGLAERVSEFNLPILVITGDDDRIVPTEQS
                     LRLAEELPNAELAVIPQCGHLPHEECPDDFMQAVKGFLGK"
BASE COUNT         1428 a         1310 c         1342 g         1598 t
ORIGIN      
        1 tggctggaag cggcgccttt tttgggcaag ctactacctt gattgctgtt ttatttctgg
       61 gcggtgttca acttatctcg ctcggtattc ttggcgagta tgtcggtcgt ctctacgacg
      121 aagccaaagg tagacctctc tatatcgttc gtgacgcgcc tgaagaatag tattcttaga
      181 tcgttgattg aaaggtcgtt gacaattatt gtcaacgacc tttttatccg tttatgtaaa
      241 aatatagtta ataggcagct atgttcaaat ctctttcctt tttgaaagac cgtacatttc
      301 taaacgagat gttggtcatt gcattgccaa tttcgtttca acaacttatc aatgcttcgc
      361 ttaatatgat cgacgtcatc atggtggggc agttgggcga aacatccatt gctgcacttg
      421 ggttgtcgaa ccaggtcttc ttcgtattta tattgctgct ttttggtctt actagcggta
      481 tggcaatttt tactgctcaa ttctggggca agcaagaagt agacccaatt cgcaaagtgt
      541 tgggcatgag tttggcaatc acatctgcca ttgctttgct gtttacgctt gccgctacac
      601 tgattccacg tactgttctt ggtctttata cacacgataa ggaagtgatt gaaatcggtg
      661 cttcatatct cagtattgtc ggtttctcct acattcctgt ggcaattgct acttcatata
      721 tcgcaacgtt gcgcagcatt caactcgtca agctgacagt gattgccact atctccgctt
      781 tgatctttaa aaccatcctt ggatacattt tgatttttgg cgtttttgga ttgcccgccc
      841 tcggggttcg tggagccgcc attggcaccg cctctggctg gaccttggaa ttgatcctac
      901 tcattatcct tgtctacgct cagaagactc ctctcgctgc caacccgttg acctttttct
      961 ctttcgactt ttctttcttt gggcgcgttc taaagaccac cttgccggca cttgccaatg
     1021 aaatgttctg gtcgttgggc attacaactt ataacgctat ctacgcccgc atcggcacag
     1081 actctattgc cgctatcaat gtcaatgcga cgattgaaga gcttggtttc gtagtattca
     1141 tggggttggg gaatgcctgt gccgtcatgg ttgggaaccg tattggtgct gggaagaaag
     1201 aagaagcctt tgaaactgtt cgacgagtag tgatccttgc tcttctttct gcatggacgg
     1261 tcggtctaat cgttttgttc ttgcgtgata ttgttgtgaa cttatacgac ctttcaccta
     1321 gcggcgaata taacgtccgc atgctgatgt tgatgatggc atgtactttg tggatacgta
     1381 tgttcaactt ctcgacgttt atcggcgcct tgcgggctgg cggtgatacc cgctttgcac
     1441 ttctaatgga attatgctct atctggctta ttggcgtccc ggcagcatac attggggcat
     1501 ttgttctgca tttaccagtc tattttgttt atctaatggt tgcattggaa gaagtcgcga
     1561 aagcttttgt gagcggatgg cgtgtctatt cgcgaaaatg gatacatgac cttgtgaatg
     1621 tctaaaaaag aggtgctctc aaccgagagc acctcttttt gttattcaat ttcaagcttt
     1681 actgtttcta gtttttgtct ttgccatcgc cgccagataa agaaaatacc tgtgaaaaat
     1741 acggcaatgc caaaggcata aggtcctatc tgtttgacga tattgctgat aagatgccac
     1801 tcatgcccaa ggaagtaacc aacactgatg aatactgtac accaaaggta tgcacccaag
     1861 aaagtagcta ttgcaaacgt gaggtagggc atacgcgtca gcccggcagg aatggcaacc
     1921 aacattcgaa tgcctggaat tagcctgcca aagaaaataa accgtgtgcc atgcttttcg
     1981 gcaaggcgct caactcgttg catgtggcgc aggtcgatgc gaaaaaattt agtaacccgc
     2041 tctactacag ggcgtccgcc gaggcgtgct gcccagtata agatcgatgc tcccattaca
     2101 cttccgaatg cggcgaaaag ccctccaaga aacacggtag aaaaaggcaa actatgtgca
     2161 tcaatcaaca tccagccagc aagggcaagc acgacctcac taggggcgat gccagttgag
     2221 ttttcaacaa tcatgatgat ggtgacgcca aaccaaccta gctcatcata tagatgttga
     2281 agtaaattta agatattctg ctctatttga ttgatcattc tattcttgtc tctagatcta
     2341 aactggcttc tatcttacat aaaaaagatg agccatggtt taatcctaat gagagaaaaa
     2401 ttagttaaga tttactttga tcgtaacgaa gcttgagccg cggacttttc ctaaaaagcc
     2461 aaatacattg aacattactc cgtacttttt gcgaaaaagg tcttttgtgt agtttatttc
     2521 atcctgtgta ttaagaactg tagcattggc atcaatccat tcgccaatag gttcgcctga
     2581 agctgtggat ggtgcgatgc gcactttgcc gtcccgacgg atgcgcttgg ttttgccggc
     2641 atccgcttgg gtccagatac gaagcgtttc tccatcctgt gcgaaccaaa ctggagtttt
     2701 gactcctccg ccatttttac gaaacgtctc aaggtttatg tattgttgtt ttttgaattg
     2761 ttcaatgttc ttcatggtag acctttgtag ttgtttgatt tccaatcctc aaagcgctgg
     2821 gtgccaagtt ttgaaagaag caatgtcatg ccaaagccaa tgaggacgtt ggtgatgaaa
     2881 aagaataaaa cttcttctaa tggcagaata ttaaagaaga gaatacctgt ggtctgttcc
     2941 ttcgaaatag accaggtggt gtctgtgaga gcgataatat ccattagaga aagatataag
     3001 cctgggacaa atatcgtgat gaaaaccaac ttgcggtaat gccagaggat atcggcgcca
     3061 tacagcattt gtgggaagat ggcaggaaac gcccagaaca gaataattga gagataggtc
     3121 aattccacat cgccgaagaa gaacagatag gtgaagatga cccagaaaat aaacagaaca
     3181 ctgaatgaga tgatgcgccc ggttttgtta ggcttgaaag tgttcgtcgg ttcagggatg
     3241 cgacgtgcaa ggaaccacca ccataatccg gcgaggatgg tttctagcac aaagaaggtg
     3301 tactcttcaa taggtacgta accaatgaca atgcctgtca caagattggg gttgtaatac
     3361 caaacgccgg tggcaactag atagttatcc catggcgtgg tatacaccac ggcaaggacg
     3421 atatgaatca gaatcgcgac ccatacggct ttaccattgt taaaaccaaa tgtggggcga
     3481 ttgtttttat tatcccacca tgtgatgacg agaaaaataa ggatgggaat gacgagaaag
     3541 cgtaagagaa aaccaaagta tgtcattgta gtttgttagt cacttagtct aatagtttga
     3601 tagtcagata gtcgtttgta taagtcatac ttcactgcac actggctaca gtccttctct
     3661 tcactttcat ctttacgccc ggtctgggtt ccagtgtggc acccatgtgc gcgtgaatag
     3721 tatgaccagt gaattcgaat gtgaagttct tgagcaagtg agcgaggacg atccgcgcct
     3781 ctgcctgacc gaatgccgcg ccaatgcaag cccgtggacc gccgccaaac ggaatgtaac
     3841 tcataggagg agttttccgt ccatgggcga aacgctcggg gcaaaattgc tcagcatttt
     3901 cccagacctc ggggtcgcga tgggtgaggt aaatggagta gaacatacgc tccccagcag
     3961 ggaccttgcc ttctgcgaat tccatttctt ctgccacacg acggttacca atatgaatag
     4021 gcgggtacag tcgcagtgct tctttgatga cgttatctaa tagttgatat ttgccttctg
     4081 cacggtcaac ttcttgcacc acatttgcgt ggatttcagg atgctgccca agcaaggcaa
     4141 aggtccatgc aaataaggcg gtggaggtat catgcccggc aataagcatg gtcagcattt
     4201 ggtcgcggat gatgtcatca ctcaacccgg catcgatcaa atgttgcaga aaatcatttg
     4261 tttttctttc ctctttcctt ctttcatcga tgatcgcata catataatca tcgatcacct
     4321 tcaacgactt cccgtaccca agacgcggaa tcttccgcca taaaatccac attcccggtg
     4381 agacgtattg aatggttttg aggattggtt tccagatctt cggaatgtca ttccaggcat
     4441 ctttgttgaa caaggtctgc atgatgatga gcagagcaat ctttcgccct tccactaaca
     4501 tatccactat ttgaccatct tgccaggtgg atataactcg atccacttgt tgaatcatca
     4561 tctcggaata tttcgataac tgtgagggat ggaatggcgt ttccatcaga cttcgatagt
     4621 ggtcatgctc ttcgccgtcg gtcaccagta cgccgcgccg cagcaggtcg gtcactgggt
     4681 cggtgttgcg ccatagcaac ttcttccgat cagtgattaa gaccttgcgt gccgcttccg
     4741 ctccgaagac gacatatgga ctgaacccgg ggatgggaat ctgaaagaag cgccccacat
     4801 gctttgccat cactttcaat ggacctaatg gcgagcgttc gtggaagagc gctttcaaag
     4861 tggcgcgacc ggtttcagga gaaagttgct gtgccatgat tactttccaa gaaagccttt
     4921 cactgcttgc atgaaatcgt ctgggcattc ttcgtgcggc aaatgcccgc attgagggat
     4981 gacagccagt tctgcattgg gtagctcttc tgcaagtcgt aaagactgtt ccgttggaac
     5041 gatgcgatca tcatcgccag tgataaccag gatgggcaaa ttgaactcgc taacccgttc
     5101 tgccaatccg ctttcgcggc tggcgagtgt caactcccat aaggctttgt cccagttttc
     5161 cacttgtaac ggtttttgat acccctcaag cgtggactgt ggaatcttac ttgggtcatg
     5221 ccaggcagtc ttgatgaatt ccacgccttg cgcctgaagt tgacgtgaga tcaacggacc
     5281 gacatgacgc atctgtggtg tgcccagcag tggacgaatc catgcgggcg cgcctccacc
     5341 tgcgtacacg gctgggtcaa ccagaatgag gctttcaatt cgctcggggt atttcaacgc
     5401 cgtcaacata gagacggttc cacccgcaga attcccaacc aaggtggctt tttcaattcc
     5461 aagggcatcc atcaaaccga tgacgagttc cacttgcgaa tcctgactgt atgggctttc
     5521 accttcccat tccaacggac gttcagtgag accaaaggca gggcggtcat atgcaatgac
     5581 tgtgccatac tctgcgaggg gagttgtcac ttcatgccac gagaacaaac tcgcgccaaa
     5641 gccatgtagc aaaatgaaaa caggcttgcc tgttccat
//
LOCUS       DAOQFT010000082         5676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7159_length_5676_cov_5.926170, whole genome shotgun sequence.
ACCESSION   DAOQFT010000082 DAOQFT010000000
VERSION     DAOQFT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_5676_cov_5.926170"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLR93_03255"
     CDS             <1..529
                     /locus_tag="PLR93_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNH26007.1"
                     /translation="HIKKHPEAVMKGLETITKSTVKFIEAVHEIGADGIFYAIQHAQG
                     RILELDEYKNIGLPYDQQVLKPAEEFWCNLLHLHGKDVYFSLLRLMNFQIVNWHDRET
                     YPSLSEAKSLYRGVVCGGVRQETLYSGTPAQVKEEAANAIQQTKGKRFILGTGCVVYY
                     QSAHENIMAAIESTR"
     gene            526..1080
                     /gene="rsmD"
                     /locus_tag="PLR93_03260"
     CDS             526..1080
                     /gene="rsmD"
                     /locus_tag="PLR93_03260"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNH26008.1"
                     /translation="MSLRVISGTAKGRKLKAVPGDTTRPVMDRVKESLFNILAGDVID
                     SNWWDLFAGTGAIGIEALSRGAAFVKFTDMNRMPIETIKDNVETCKFTAQADIRRGDA
                     FTLLAGTADQQFEYIYIAPPQYKKMWSEALNLVDDNMEWLTEDGTVIVQIDPTEYEEI
                     ELHNLVEGEQRKYGGTLLIFYDRK"
     gene            1092..1433
                     /locus_tag="PLR93_03265"
     CDS             1092..1433
                     /locus_tag="PLR93_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNH26009.1"
                     /translation="MILTVLQYIACVLTILVGAYSLFAPKNAGKFTGLTPEGGRGITE
                     IRVVLGMFFVALGAYPILFPSDASFHMLGFAYVLVGIARLISIFVDKSSEQSNWISVA
                     SEFILGAILLF"
     gene            complement(1434..1673)
                     /locus_tag="PLR93_03270"
     CDS             complement(1434..1673)
                     /locus_tag="PLR93_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26010.1"
                     /translation="MTDKQSRNETHGCIVCGKLHQMLVVYNADGTFFDLKVMSSDGKK
                     VVHSKRPLVACEKHTKEEVESAVARVYGTQQEDDE"
     gene            complement(1730..2422)
                     /locus_tag="PLR93_03275"
     CDS             complement(1730..2422)
                     /locus_tag="PLR93_03275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH26011.1"
                     /translation="MAKKKLAKKNNPQTASSQKTIQYVAGGVILLVVAFFVWQYFPKS
                     APKQDAAICEQFADIPVADQYDSAPPMKVDAAKKYFATVEMENGGQFKMELYPDKAPI
                     TVNSFVFLSCKGFYNGVTFHRVLEGFMAQGGDPTGTGAGGPGYQFVNEDSDLVFDKAG
                     VVAMANAGRDTNGSQFFITFAPADFLNGGYTIFGQVTEGMDVVNAITRRDPQQNPTFE
                     GDKIISVLISEE"
     gene            2504..3367
                     /locus_tag="PLR93_03280"
     CDS             2504..3367
                     /locus_tag="PLR93_03280"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HNH26012.1"
                     /translation="MRSNSFSCMLAKMQSYFKRQIAFPQDHGSWVFIFSPLMVGLFAG
                     KTFQHATIYLITAALSAFLIRQPITILVKAISGRRSAADLPAARFWLLVYGIIAAAAL
                     IGLILSGYSYLIYLAVPGAPVFAWHLWLVSKRAERKQLGVEVLATGVLALVAPAAFWV
                     GIGKYDPHGWWLWLFTWLQSAASIVYAYLRLSQRDMSKEQAQATSRAAWWEMGSRALL
                     YTSFNLLLSAGLGISALVPLFIFVPFLVQWLETLWGITHPSVGWKPIQIGMRQLVVSV
                     LWTALFIVFWR"
     gene            3370..3603
                     /locus_tag="PLR93_03285"
     CDS             3370..3603
                     /locus_tag="PLR93_03285"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNH26013.1"
                     /translation="MDELKYDLLTELTNRMQADLLESFLEANGIDVELFQESVGHSIY
                     PVTIDLLGRVQVFVPKDKIEQAKILLEEFNAEE"
     gene            3607..3951
                     /locus_tag="PLR93_03290"
     CDS             3607..3951
                     /locus_tag="PLR93_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNH26014.1"
                     /translation="MKNTVLLCTKFGMGQTDIASLNQKLISKYLSLLLEGNQLPAAIC
                     FYTDGVKLVTGISPVLEQLSQLEQKGVRLIICSTCLDEMGISRDDVKVGIVGGMPDII
                     EAQTKADKVITL"
     gene            3965..5113
                     /locus_tag="PLR93_03295"
     CDS             3965..5113
                     /locus_tag="PLR93_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH26015.1"
                     /translation="MNFDLTEEQKTWKKIVHDFAEKELRPKAREVDEKEEFNWEAFRK
                     AGPVGLASMSIPEEYGGSNVDMISNAIAFEELGWGCGSTALAFTAHNGLGTAPIALYG
                     SEELKKKWLPLVGNGKNKLGALALTEPGAGSDIQGGVTTRAEKDGDHWVINGAKMWNT
                     NASIAEYIITLVRTDPKGGSKSLSMILVPTDSKGLSIAPAEKKMGLRGSPTHAVTFDN
                     VRVPLGNLIGPEGRGLQQTLSTLDAGRISIGAISLGLARAGMEAAIQYSKERKAFNQS
                     LADFQAIQFKIANMEMEIELARTYIYKAAWLKDEGRPYSKEAAIAKLYATEMAERVLY
                     DAIQIHGGYGYSREYNVERMYRDARLMTIGEGTSEIQRLVISRHVLAG"
     gene            complement(5147..>5676)
                     /locus_tag="PLR93_03300"
     CDS             complement(5147..>5676)
                     /locus_tag="PLR93_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH26016.1"
                     /translation="AIAPIYARVTYSKKEMMREVASLPVQGRLLDVGGGTGRVSSAIR
                     EYVDEVIIADVSFGMLARAPRPSLQPVCGASETLPFADNFFERVIIVDALHHVANHAH
                     TAHEMFRVLKPGGVLVIEEPDIRTFGVKLIALAEKLLLMRSHFLAPDGIMKLFDIGGK
                     ETKSVDGTAWVIIKK"
BASE COUNT         1556 a         1282 c         1351 g         1487 t
ORIGIN      
        1 acacatcaaa aaacatcccg aagccgtgat gaagggattg gaaaccatta caaaaagcac
       61 ggtcaagttt atagaagctg tacatgaaat tggtgcagat ggcattttct acgcgattca
      121 acatgcgcaa ggacggattc tggaattgga tgaatacaag aacattggct tgccttatga
      181 ccagcaagta ttaaaacccg ctgaagaatt ttggtgcaac ttactccatt tgcatggcaa
      241 ggatgtgtat ttctctttgt tgcgcctaat gaattttcag atcgttaact ggcacgaccg
      301 cgaaacatac ccatcgttga gtgaggcgaa gagcctatac cgtggcgtgg tatgcggagg
      361 cgtccgccag gagacgctct actcgggcac cccggctcag gtcaaggaag aggcggcgaa
      421 tgctattcag cagaccaaag gcaaacgctt tatcctcggc acgggctgcg tagtctacta
      481 tcaatcagcg catgaaaata ttatggctgc cattgagagc acacgatgag cctaagagtg
      541 atttcgggaa ctgccaaagg cagaaagtta aaagcggtgc cgggagatac cacgcgtcca
      601 gtgatggatc gtgtcaaaga gtcgctattc aatattttgg caggtgatgt aattgactca
      661 aactggtggg acttgttcgc aggcacaggc gcaatcggta tcgaagcgtt gagccgcggg
      721 gctgcattcg taaagtttac agatatgaat cgcatgccca ttgaaactat caaagataat
      781 gtagagactt gcaaattcac agcacaagca gacattcgcc gtggcgatgc atttacgctg
      841 ctagccggga cagccgatca acaatttgaa tatatttata ttgcgccgcc gcaatataaa
      901 aagatgtggt cggaagcgtt gaatttggtc gatgacaaca tggaatggct gaccgaagat
      961 ggaaccgtca ttgtgcagat cgacccgacc gaatatgaag aaatcgaatt gcacaatctc
     1021 gtggaagggg aacaaagaaa atatggcgga acgttgttga tcttctacga tagaaaataa
     1081 aaggagcagc catgattttg acagtccttc aatatatcgc ttgtgtgttg accattttgg
     1141 tgggcgctta ctctttattt gccccaaaga acgccggaaa atttacaggg cttaccccag
     1201 aaggcggacg tggcattact gagattcgcg ttgtgcttgg aatgttcttt gtagcattag
     1261 gagcatatcc catcctcttc ccctctgatg cgtcgtttca tatgctcgga ttcgcatacg
     1321 tgctggttgg gattgcaaga ttgatttcaa ttttcgttga taaatctagt gagcaatcaa
     1381 attggatcag tgttgcaagt gaatttatcc ttggtgcgat tcttttgttt tagctactcg
     1441 tcatcttctt gttgtgtacc atagactcgt gcaacagcag attcaacttc ttcttttgtg
     1501 tgtttttcgc aagccaccaa cggacgcttg gaatgcacaa ctttctttcc atctgagctc
     1561 attactttga ggtcaaaaaa ggttccatct gcattgtaga caacaagcat ttgatgtagc
     1621 tttccacaaa cgatacagcc gtgtgtttca ttacgagatt gtttatcggt catcatcact
     1681 cctaacgaaa agagacagtc accctgaaag tgactgtctc ttttttgaat tattcctctg
     1741 aaatcaatac agagataatc ttatcgcctt caaaggttgg gttttgttgc gggtcacgtc
     1801 gggtaatcgc attgaccacg tccatgcctt cggtcacttg accaaaaata gtatagccgc
     1861 cattcaagaa atctgccggg gcaaaggtga taaagaactg gctgccgttc gtatcacgcc
     1921 cagcatttgc catcgccacc acaccagctt tatcaaagac caagtcgcta tcttcattca
     1981 caaactgata gccaggtccg cccgcaccgg tgccggtagg gtcaccgcct tgtgccatga
     2041 atccttcgag cacacggtgg aaggtcacgc cgttgtaaaa gcctttgcaa gacaagaaaa
     2101 caaagctatt gacagtgatc ggcgctttat cggggtaaag ttccattttg aactgaccgc
     2161 cattttccat ttcaacagtt gcaaaatatt ttttggcagc atccaccttc atgggcggag
     2221 ctgagtcata ctggtctgct acaggaatat cagcaaactg ttcacaaatc gcggcatctt
     2281 gtttaggggc actcttcgga aaatattgcc aaacaaaaaa tgcaaccaca agcaaaatga
     2341 caccgccagc aacatactgg atggtctttt gtgaagatgc ggtttgtgga ttatttttct
     2401 tagccaactt cttttttgcc atacgaattt ctctcctaaa aaattttctc aattataaca
     2461 accttgacct tctttcctta ataaacacat gagagatggc accttgcgtt cgaattcttt
     2521 ttcctgtatg cttgccaaaa tgcaatcgta cttcaaacgg caaatcgctt ttccgcaaga
     2581 tcacggctca tgggttttca ttttcagccc gttaatggtc ggtctttttg ctggcaaaac
     2641 ctttcaacac gcaacgattt acctcatcac tgcagcgtta tctgcattcc tgattcgaca
     2701 accaatcacg attctcgtca aagccatcag tggacggcgc tccgctgcgg atcttcccgc
     2761 cgcccgtttc tggttgctgg tctatggcat catcgccgcc gcagccttga tcggacttat
     2821 cctctccggc tacagttatc ttatctactt ggctgtgcca ggggcaccgg tctttgcatg
     2881 gcatttgtgg ctagtaagta aacgcgctga acgaaaacaa ctgggtgtag aagtgctagc
     2941 aactggagta ttggcactgg tcgcacccgc tgccttttgg gttggcattg gcaaatacga
     3001 cccccatggt tggtggcttt ggttatttac atggctgcaa tcagctgcca gcattgtgta
     3061 tgcttatctc cggctgagtc aaagggatat gtcgaaggaa caggcacagg cgacgagtcg
     3121 agccgcgtgg tgggagatgg gaagccgcgc attgttatat acatccttca acctgcttct
     3181 ctcggcgggc ttggggatat ccgctcttgt gccgctgttc atcttcgtcc catttttggt
     3241 gcaatggctg gaaacgcttt ggggaatcac ccatccatct gtgggttgga agcctatcca
     3301 aattggtatg cgccaattag ttgtgagcgt tctatggact gcattattta tagtgttctg
     3361 gaggtaaaaa tggacgagct aaaatatgac ctattaaccg aactcacaaa tcgcatgcaa
     3421 gcagatttac tggaaagttt tctcgaagcg aatggcattg atgtggagtt atttcaagaa
     3481 tctgtggggc actccatcta tccggtcaca atcgacttat tagggcgcgt gcaggttttt
     3541 gtacccaaag acaaaataga gcaggcaaaa atactgcttg aagaattcaa tgctgaggaa
     3601 taagaaatga aaaacaccgt tctactttgt acaaaatttg gaatggggca aacggatatt
     3661 gcctctctaa atcagaaatt gattagcaag tatttgagcc tgttactgga agggaatcaa
     3721 cttcctgctg ccatttgttt ttatacagat ggggttaagc tggtaaccgg tatttctccg
     3781 gtactggagc aactttctca attggaacaa aaaggggttc gccttattat ttgctctacc
     3841 tgtcttgatg aaatgggaat ttctcgtgac gatgtaaaag taggaattgt aggcgggatg
     3901 cccgatataa ttgaagcaca aacaaaagcg gataaagtca ttacgctata aattaggagt
     3961 tattatgaat ttcgatctaa ctgaagaaca aaaaacgtgg aaaaagatcg tccatgactt
     4021 tgctgaaaag gagttacgcc ccaaagcacg tgaagtggat gaaaaagaag aatttaattg
     4081 ggaagcgttc cgcaaggctg gacctgttgg tttggcaagt atgagcatcc ccgaagaata
     4141 tggcggctcc aatgtagaca tgatctcaaa tgccattgcc tttgaagaat taggctgggg
     4201 ttgtggctca acggcgcttg ctttcacggc tcacaatggc ttagggacag ctcctattgc
     4261 tttgtatggt agtgaagaac taaagaaaaa atggctgcct ttggtaggca acggaaagaa
     4321 taaacttggc gccttagctc tcaccgaacc tggtgctggt tctgatattc aaggtggggt
     4381 caccactcgc gccgagaaag atggcgatca ctgggtgatt aatggcgcaa agatgtggaa
     4441 caccaatgcc agtatcgccg aatacatcat caccctcgtc cgcaccgacc cgaaaggcgg
     4501 ttcaaaatcg ttgagcatga tcctcgtgcc aaccgactct aaaggcttga gcattgcacc
     4561 tgccgaaaag aaaatgggct tgcgcggctc ccccacccat gcagtgacct tcgacaacgt
     4621 gcgcgttccg cttgggaact taattggacc ggaaggcaga ggcttacaac agactctttc
     4681 cactttggat gcaggacgca tcagcattgg tgcaatttca cttgggttgg cgcgcgctgg
     4741 catggaagct gcaattcaat attccaaaga acgtaaagcg ttcaaccagt ctctggctga
     4801 ttttcaagcc atccaattca agatcgcaaa catggaaatg gaaattgaac tagcgcggac
     4861 gtacatttac aaagccgcct ggctcaagga cgaaggacgt ccgtatagca aggaagctgc
     4921 aattgccaag ttgtatgcta ctgaaatggc agaacgagtg ctttacgatg ccattcaaat
     4981 ccatggtggg tatgggtata gccgtgaata caacgtggag cgaatgtatc gcgacgcacg
     5041 tctcatgacc attggcgaag ggaccagtga aattcagagg ctggtcattt caaggcatgt
     5101 gttagctgga taataaaaaa gcggaggctt ccatgcctcc gcttttttat tttttgatga
     5161 tgacccaagc tgttccatcc accgacttgg tttcttttcc cccaatatca aataatttca
     5221 taattccatc tggtgcaagg aagtgactgc gcatgagcag cagtttctct gctaaagcaa
     5281 ttaacttcac cccaaaggta cgtatatccg gttcttcgat gacgagcacg ccaccaggct
     5341 ttagcacacg aaacatttca tgagcagtat gagcatgatt cgccacatga tgcaaagcat
     5401 ccactatgat gacccgttca aaaaagttat cagcgaaggg taatgtttca gaagcaccac
     5461 acacaggctg aagggaaggt cgaggcgcac gggctaacat accaaatgaa acatcggcaa
     5521 taatgacctc atccacatac tcacgaatgg cagaagaaac acgtccggtg ccaccgccta
     5581 catccagcaa acgtccttga actggaagag aagcaacttc gcgcatcatt tcttttttag
     5641 agtatgtgac acgcgcataa atcggagcaa tggcag
//
LOCUS       DAOQFT010000083         5660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7182_length_5660_cov_6.463515, whole genome shotgun sequence.
ACCESSION   DAOQFT010000083 DAOQFT010000000
VERSION     DAOQFT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7182_length_5660_cov_6.463515"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PLR93_03305"
     CDS             <1..1208
                     /locus_tag="PLR93_03305"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNH26017.1"
                     /translation="DLMKVLLVGVGGVGEAIAAIAKPREWMELMVLADYNVKRAEEVQ
                     KKLGDPKRFPVEFIDASNQKNIEDLAKKHQVDLIMNSVDPIFNQQIFDAAYNAGVKYM
                     DMAMTLSEPHPTDPFNKPGVKLGDYQFEKAKEWEKKGLLALVGFGVEPGMADVFARYA
                     ADHLFDEIEEVGIRDGANITIEGYDFAPNFSIWTTIEECLNPPVIWQNGDWHTTAPFS
                     EPEVFDFPEIGPVEVVNVEHEEVLLVPRWVKAKRVTFKYGLGNEFIEVLKTLHKLGLD
                     NKNKINVKGVQVAPRDVVAACLPDPAHLGDKMKGKTCAGTWVKGTKDGKKKEVYLYQV
                     ADNEECMKTWGCQAVVAQTAFTTVIAMDLLEHGAWKGTGVLGPEAFEPTVFMEKMADY
                     GFPYGIKNM"
     gene            1427..3334
                     /locus_tag="PLR93_03310"
     CDS             1427..3334
                     /locus_tag="PLR93_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNH26018.1"
                     /translation="MPNGYNGKILHVDLTKGSLTVEEPNEAFYRKYLGGSAMGMHYIL
                     RDMPKGADPLGPENVLTLFTGVTTGAAISGQSRLNANAKSPISGGIGDSQSGGFFPAE
                     LKFAGFDGIVVKGKSPKPVYLCIIDGKFELRDASHLLGKLSGEVDDIIHKEVDPKAEI
                     LQHGIGAENGVLYSSLVSMSNRHNGRTGMGLVMASKNLKAVVVRGTGKIKLANQKALT
                     ELNRIGPKAIPENGDMDGLAKFGTAVVVLFNNTIGTLPTRNYNEGQFEGCEPISGEKM
                     AETVLKERDTCYACVVKCKRVVEIKDGPYKVDPRYGGPEYETLGTFGSYCGVDDLAAV
                     SLANQICNEYAVDTIAAGATIAFAMECYEKGIITKEQTAGLDLKFGNAEAMIAALNML
                     VKGEGEFGKTLGLGSERAAAKWGNGADECLITVKGAEAPAHMPQAKRSLALIYAVNPF
                     GADHQSSEHDPYYEEGVGDFNLDRLKQIGLGSPQPAYSLTEEKVRFAYETEVFYSMLD
                     SAELCQFVYGPTWTVYDGEQTAQMISSVTGWDITLNELMEVGRRRLDLFRVFNAREGL
                     ARNADKLPKKFFKELKGTGPTAGFALTHEEVESALDTYYKMAGWTNNGIPTRETLKKH
                     DVEWAAEYLPA"
     gene            3422..4702
                     /locus_tag="PLR93_03315"
     CDS             3422..4702
                     /locus_tag="PLR93_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH26019.1"
                     /translation="MTNQLYQDNFSHVTPAWSRIFNFVADRAEGSYIYTDDGKKLLDF
                     TSGIGVTNTGHCHPKVVEAIREQAGLFLHAQANIVIHKPMLQLIEELRKVVPPSIDSF
                     YFANSGAEALENAVKIAKAVTGRQNIIVFNGSFHGRTHATMALTTSKTIYRTGFAPLP
                     AGIYVAPFPYAFNLKMTEEQASQYALEQLEYLLASQTAPKETAAILIESVLGEGGYVV
                     PPASFMKGLREICDKHGIMLIFDEVQSGFGRTGKWFALEHFGVVPDIITAAKGIASGM
                     PLSGVFSRTEIMKKVDTGSIGGTYGGNAVACAAGVATIRAMREEKMLENATERGIQLM
                     TGLHKLQEEYPQIGDVRGKGLMVGTEFIVDGSQAKAKPLVKEIIHKAEEKGLLLLSCG
                     TYDNILRWIPPLNVSSEQINAGLKIFGEAVKGSV"
     gene            complement(4699..>5660)
                     /locus_tag="PLR93_03320"
     CDS             complement(4699..>5660)
                     /locus_tag="PLR93_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH26020.1"
                     /translation="AVDAILAEMELEKFNFTTVFKNWSTEDFQNSQPSDDYVFEYVFK
                     SEAGRMLIHFTARDLSYITVEFLYDHDQQSSEEWVLAMNHKLRSKFGAPKKPQFKVLM
                     HQDGMFGMEEVSTNDFKSIDIAELYNDDFVEVDKAISTFMGKQESGIILLHGEPGTGK
                     TTYIKHLICKFIDREFIFIQNDFVSDLLKPNFIAFLLQNKNAVLIIEDAEKVVVPREH
                     SSEASVVSTILQLTDGLFSDFLNIKIICTFNTDIERIDKALLRKGRMIAKYNFSALSP
                     AKTAALAQKLGHEHVEGSLTLADIFGYDKLDFKEAAKRKIGFV"
BASE COUNT         1460 a         1197 c         1537 g         1466 t
ORIGIN      
        1 cagatctcat gaaagtattg ctagttggag tgggtggagt tggggaagcc atagccgcca
       61 ttgccaagcc gcgcgagtgg atggagttga tggtgctggc agattacaac gtcaaacgag
      121 cagaagaagt gcaaaagaag ctgggagacc ctaaacgctt cccggtcgaa ttcattgatg
      181 ctagcaacca gaaaaatatc gaagacttgg caaagaaaca ccaagtggat ctcatcatga
      241 attcagtaga cccgatcttc aaccaacaga tcttcgatgc cgcctacaat gctggcgtga
      301 aatatatgga catggcgatg accctttccg agccgcaccc aaccgacccg ttcaataaac
      361 cgggggttaa gttgggcgat tatcaatttg agaaagccaa agagtgggag aagaaaggtt
      421 tgttggcgct tgttgggttt ggcgtggaac ctggcatggc agatgtcttt gctcgctatg
      481 cagctgacca tctctttgac gagatcgaag aagtgggcat tcgcgatggc gctaatatca
      541 ccattgaagg ctatgatttt gcgccgaatt tttcaatttg gacaactatt gaagaatgtt
      601 tgaatccgcc cgtcatctgg caaaacggag attggcatac cacggctccg ttctctgagc
      661 ctgaagtgtt cgacttccct gaaattggac ccgttgaagt cgtcaacgtg gagcatgagg
      721 aagtattgct cgtgccgcgc tgggtgaagg caaagcgtgt gaccttcaag tatgggttgg
      781 ggaatgaatt tattgaagtg ttgaaaaccc ttcataaact tggcttggat aataagaaca
      841 agatcaatgt gaaaggcgtg caagttgcgc cgcgagatgt ggtggcggct tgtttaccag
      901 acccagctca cttgggtgac aagatgaaag gtaagacatg tgccggaacc tgggtaaaag
      961 gcacaaaaga tgggaagaag aaagaagtat atctgtatca ggttgcggat aacgaagaat
     1021 gcatgaagac ttgggggtgc caggcagtgg tggctcaaac ggcatttacc actgtgattg
     1081 ctatggattt gctggaacat ggcgcttgga agggcacagg tgtgctcgga cctgaggcgt
     1141 ttgagccgac tgtcttcatg gaaaaaatgg cagattacgg cttcccatac ggcattaaga
     1201 atatgtagtt tataaaaggg cgactcttta cgggacgccc ttttgcttcg ggtgtgaaag
     1261 gttcgagaaa gcggtatccg ctgtcagtta ggaaggaagc gcggcgggga gtgtaggaaa
     1321 aaacttaatc gaagcggtta agctgtcgct ttcggaaact gcctgttgtg agaagtcaga
     1381 cccgcttgtt ataattacca tcatcaattc ttacggagga gtaaccatgc caaacggtta
     1441 taacggaaag attttgcacg ttgatctgac gaagggctct ctgacggtgg aggagccgaa
     1501 tgaggctttt taccgcaagt atttaggcgg gagcgcaatg ggaatgcact acatcttgcg
     1561 cgatatgccg aagggcgctg acccgcttgg acctgaaaat gttttgacct tgtttaccgg
     1621 tgttacaact ggggcggcaa tctcaggtca gagccgtttg aatgcaaatg ccaagtcgcc
     1681 gatcagtggc ggtattggtg attcgcagag cggtgggttc ttcccggctg agttgaagtt
     1741 tgccggtttt gatggcattg ttgtgaaggg gaagtcaccc aagcctgtat atctctgcat
     1801 tattgatgga aaatttgaat tacgcgacgc ctcgcatttg ctgggcaagc tttcgggcga
     1861 agtggacgat atcatccaca aagaagtgga cccgaaggct gagatccttc agcatgggat
     1921 tggcgctgaa aatggggtac tgtattcatc cctcgtttct atgtctaatc gtcataatgg
     1981 gcgtacgggc atgggtttgg tgatggcatc taagaatctc aaggctgtgg tggtgcgagg
     2041 taccggcaag attaaacttg ccaatcaaaa ggctcttact gagttaaatc gtatcggtcc
     2101 gaaagccatt cctgaaaatg gtgatatgga cgggcttgcg aaattcggta cggcagtggt
     2161 ggtattgttc aataacacca ttggtacatt gccaacccgt aactataacg aaggtcagtt
     2221 cgaaggttgt gaaccgatca gcggcgaaaa aatggctgag acggtcttga aggaacgtga
     2281 tacttgctat gcatgcgtgg tcaaatgcaa acgcgtggtg gagatcaaag acggtcctta
     2341 taaggttgat ccgcgctatg gtggtcctga gtacgaaact cttggcacgt ttggttcata
     2401 ctgcggtgtg gatgatcttg cggcggtatc tcttgccaac cagatttgta atgaatatgc
     2461 cgtagataca attgcggctg gtgcaaccat cgcttttgcg atggagtgct atgaaaaagg
     2521 cattatcacc aaagaacaga ccgctggtct tgatttgaaa tttggtaatg ccgaagcaat
     2581 gatcgctgct ttgaatatgc tggtgaaggg tgagggcgaa ttcggcaaaa cccttggttt
     2641 gggctcagaa cgtgcagcag ccaagtgggg aaatggtgca gatgaatgcc tcatcacggt
     2701 caaaggcgct gaagctcctg cacacatgcc gcaagccaaa cgctctctcg cgctcattta
     2761 tgctgtcaat cccttcggcg cagatcacca atcctctgaa cacgacccat actatgaaga
     2821 aggtgtaggt gatttcaacc tcgaccgcct caaacagatt ggtttgggtt cgccccaacc
     2881 tgcatactca cttacagagg aaaaagtccg cttcgcatac gagactgaag tcttttactc
     2941 gatgctcgac tcagcagaac tctgccagtt tgtgtacggt ccgacatgga cggtctatga
     3001 tggcgaacaa actgcacaga tgatcagctc ggtaacgggt tgggatatta ccctaaacga
     3061 attgatggaa gtcgggcgcc gacgtttgga tctcttccgt gtcttcaatg ctcgtgaagg
     3121 tttggctcgc aatgctgata aattgcctaa gaaattcttc aaggaattga aggggacagg
     3181 tcccacagcg ggcttcgctc tgactcatga agaagttgaa tctgctctcg atacctatta
     3241 caagatggca ggttggacga ataacggcat tcctacccgc gaaactttga agaagcatga
     3301 tgtagagtgg gcagcggaat accttccggc ataatgtaaa tacaatctga tcactgagag
     3361 cacagagtcc aagaagaaat tcagggaaaa ctctgtgctc tttctggtca ataaggaaat
     3421 catgaccaat caactctatc aagataattt ttctcatgta acccctgcct ggagccgcat
     3481 tttcaatttc gtagcggacc gtgctgaggg ttcatatatt tataccgatg acggcaagaa
     3541 acttctggat ttcaccagtg gaattggcgt cacaaatacg gggcattgtc accccaaagt
     3601 ggtggaggcg attcgcgagc aggctggctt gttcttgcat gctcaggcaa acatcgtcat
     3661 ccacaaaccg atgttgcaac tgattgaaga actgcgtaaa gtagttccgc cttcgatcga
     3721 tagtttctac tttgccaact ctggcgcaga ggcgttggaa aatgccgtga agattgccaa
     3781 ggctgtcacg ggtaggcaaa acattattgt cttcaacggc tcctttcatg ggcgcacgca
     3841 tgccactatg gcattgacca cctctaagac aatctatcgc accggctttg cgcctttgcc
     3901 tgctggcatt tacgtagcgc cgttcccata tgctttcaac ctgaagatga ccgaagaaca
     3961 agccagtcaa tatgcattag agcaattgga atatttgcta gcttctcaaa cggctcctaa
     4021 agagaccgcc gctatcttga tcgagtctgt attgggtgag ggcgggtatg tggtgccgcc
     4081 tgcctccttc atgaagggat tgcgtgagat ctgcgacaag cacggcatta tgctgatttt
     4141 cgatgaagtg caatctggtt ttggtcgcac gggcaagtgg tttgcgttgg aacactttgg
     4201 tgttgttcca gatattatca ctgctgcaaa agggattgcc tctggaatgc cgctttcggg
     4261 cgtctttagt cgcaccgaga tcatgaagaa agtggatact ggctccattg gtgggaccta
     4321 tggtgggaac gcagttgcct gcgcggctgg cgttgctacc atccgcgcga tgcgtgaaga
     4381 gaaaatgtta gagaacgcca cagaacgcgg aatccaattg atgaccgggc ttcataaatt
     4441 gcaggaagaa tatccgcaaa ttggtgatgt gcgcggtaaa ggcttgatgg ttggtaccga
     4501 atttattgtg gatggcagtc aggcgaaagc caagccgttg gtcaaagaga tcatccataa
     4561 agcagaagag aagggtttgc tccttctctc ctgtggtacc tacgataata ttttgcgttg
     4621 gattccgcca ttgaatgtct cttctgaaca gatcaacgct ggtttgaaga tttttggcga
     4681 ggctgtaaaa ggctctgttt agacgaaacc gatcttgcgt ttggcggctt ctttgaaatc
     4741 gagtttgtca tagccgaaaa tgtctgcaag ggtgaggctg ccttccacat gttcgtgacc
     4801 tagtttttgg gcgagggcag ccgtctttgc aggggagagc gcagagaagt tgtacttggc
     4861 aatcatacgc cctttgcgta agagagcttt gtcaatgcgc tcgatatcgg tgttgaaggt
     4921 gcagatgatc ttgatgttga gaaagtcgct gaagagaccg tcggtcaatt gcaaaatggt
     4981 tgagacgacg gatgcttcag aggagtgttc tcgcggcacg acaactttct ctgcatcttc
     5041 aatgatgaga actgcattct tgttttgcag tagaaaagct atgaaatttg gtttcaacaa
     5101 gtcgctgacg aaatcatttt gaataaagat gaattcgcgg tcgataaact tgcagatcag
     5161 gtgtttgatg taagtggtct tgccggttcc gggctcacca tgcaaaagga tgatgccaga
     5221 ctcttgcttg cccatgaatg tggaaatggc tttatccact tcgacaaagt catcattgta
     5281 gagttcggct atgtcaatag atttgaaatc atttgtgctc acttcttcca tgccaaacat
     5341 tccgtcctga tgcataagga ctttgaattg tggctttttg ggtgctccaa actttgagcg
     5401 taatttgtgg ttcatcgcca gcacccattc ttcactactt tgttggtcat ggtcgtaaag
     5461 aaattctact gtgatgtacg acaggtcgcg agccgtaaag tgaatgagca tgcgcccagc
     5521 ttcacttttg aaaacatact cgaacacata gtcgtcgctg ggttgtgaat tctgaaaatc
     5581 ttcagtagac caattcttga acacggtggt gaagttgaat ttttccaact ccatttcggc
     5641 aaggatggca tccacggcat
//
LOCUS       DAOQFT010000084         5578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7338_length_5578_cov_5.040014, whole genome shotgun sequence.
ACCESSION   DAOQFT010000084 DAOQFT010000000
VERSION     DAOQFT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7338_length_5578_cov_5.040014"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /gene="typA"
                     /locus_tag="PLR93_03325"
     CDS             <1..1684
                     /gene="typA"
                     /locus_tag="PLR93_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNH26021.1"
                     /translation="LAKNTAISLVDPATNQPIKINIVDTPGHADFGGEVERVMNMVDG
                     VLLLVDAAEGPMPQTRFVLKKALEMGHKAIVVINKVDRKDAEPDRVLNETFDLFIELG
                     ASEEQADFPVIYAIGIQQRAGLTPDLGPDLQPLFDTILKHVPAPKVDVDAPLQMLITT
                     LGYDDYRGVTAVGCVFAGKIKAGQKLARIKLDGTILPETARYLYTFKGLNKVDVEESI
                     AGDIISLAGLEDIAIGETLADPTNPIALPSIKVEEPTVRMTFGVNTSPFSGKEGKWGT
                     SRKLRERLNEELRTNVSLRVADTDSAENFLVSGRGELHLGILIETMRREGYEFQVSRP
                     EVIYHKADDGVLLEPYEEVHIETSNETVGPVVEMLGARRGRMMDMQDSGQGTTRMVYI
                     VPTRGLLGFRYQFLTSTRGAGVMNTIFHSYDELAGAMNTRQSGSLVAWEPGTTTAYAL
                     KSAEERGTLFVGPGVDVYEGMVVGENARAADISVNVCKKKHLTNMRASRADMEIRLTP
                     PRQMSLDEAIEYLADDELLEVTPATFRIRKRILNTEERGKQTKKAKEQFGEE"
     gene            complement(1731..2432)
                     /locus_tag="PLR93_03330"
     CDS             complement(1731..2432)
                     /locus_tag="PLR93_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH26022.1"
                     /translation="MKRSVYLFLLTSLLLTSCSMSSSDAPSPEVATAAAMTVQAAIEG
                     NQTPLASPTSAGQSTPESTGNPMASFEDVTNCRVGPGVNYERVTQIQPGFAVEIVGYY
                     PPNYWIVQTDKGPCWVAGEFVTPSGSVSAVPTVTAPPTPTGGAPENVSLQKWDVACDY
                     ATNEAKVTIAWKDEDDEIGYRVVRNDVVIAELPANTTEFKETITLISGQTAGYSIISF
                     NATGSTQSQTISLGC"
     gene            complement(2457..3620)
                     /locus_tag="PLR93_03335"
     CDS             complement(2457..3620)
                     /locus_tag="PLR93_03335"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNH26023.1"
                     /translation="MPFYHKLGKIPHKRHTQFKKPDGKLYREELMGLEGFSSLQSILY
                     HHFLPPRVKKTADLGSAKPEYVDYGAIRHRAFKTAPTPLGADPVSGRIPLLGNNDVIL
                     GVSRAPKGKMNFFYRNSQAYETWWVHDGSGTIYSQFGKLPFRKGDYIVIPFGTTWQME
                     LDEETKFFTIENPSQIEPPKRYRNHYGQLLEHAPYCERDIRIPMELETHTERGEFEVR
                     VKVRDHISQHILDYHPHDVIGWDGYLYPWIFNIEDFEPITGRIHQPPPVHQTFEGWNF
                     VVCSFVPRLFDYHPEGIPAPYNHSNIQSDEVIYYAEGNFMSRKGIDRSDISLHPFGLP
                     HGPQPGMTEASIGKTETKELAVMVDTFHPLHLTKQALEMEKPYMETWLDGDGE"
     gene            complement(3630..4457)
                     /locus_tag="PLR93_03340"
     CDS             complement(3630..4457)
                     /locus_tag="PLR93_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26024.1"
                     /translation="MKLHMLVTGISLVVLVGCAPTATATESPSPTPLAVEPSATNPPT
                     ETETVSPSAFEAENISTFNELGIAIGFNYPEGFSQSISTSFNTTYSPVAPYDLPYPQN
                     AQIIFTDYSGGDGFTINGLRVFLADEVNALETGAVESVNAVLEGEGDHHVDFPRLAGA
                     GSIIDAQVLPLAFQNGNGYRYLVAKSFSADPISSTKLTYMYQGVTNDGKFFVSFIMLV
                     DASFLSEYVGQTLTTPEEFETYFQDVNNLVETSSGDQFTPSLNTLDELISSIVILEK"
     gene            complement(4471..4860)
                     /locus_tag="PLR93_03345"
     CDS             complement(4471..4860)
                     /locus_tag="PLR93_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH26025.1"
                     /translation="MTETVITVRPQGTHLSKQQLPNFEGISAKTAGSKHLCMHLVVIP
                     PGGKAVAHYHDGYETVIYIIEGKAETRYGHNLEHSTINEAGDFLFIPPNLPHQPVNLS
                     DTEQVIAVVARNDPNEQESVVVYEVKE"
     gene            complement(4869..>5578)
                     /locus_tag="PLR93_03350"
     CDS             complement(4869..>5578)
                     /locus_tag="PLR93_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26026.1"
                     /translation="PEATGISVSFQNVQFVIPEGLASGASSEVIPAADEATAGPWGVA
                     PEYIEFVLMDYSPRDEFKPPVIRIYPAQEYAAVNSWAQGSLAKLQAILASPSMSMTND
                     NLPTIPFNGAAAQQYAAQAKIVSFANGSGVRMTSQYAQFPGPILKDGSYYHYEGLTND
                     GKYMVAIMVPVTLPIQATAENPSADGVVYPSDISDTIALDAYFQGVTDLLNAATPDSF
                     QPNLNVLDALIQSIQIK"
BASE COUNT         1391 a         1266 c         1409 g         1512 t
ORIGIN      
        1 tctcgcgaag aacactgcta ttagcttagt agaccctgct actaatcaac cgatcaagat
       61 taacatcgta gacactcccg ggcatgccga ttttggcggg gaagtggaac gcgtgatgaa
      121 catggtggac ggtgtgcttt tgctcgttga tgctgccgaa ggtcccatgc cgcagacgcg
      181 ctttgtgttg aaaaaagcgc ttgagatggg tcacaaagcc attgtggtca tcaataaagt
      241 cgatcgcaaa gatgctgaac cagaccgtgt tctgaatgaa acctttgact tgttcattga
      301 acttggcgct tctgaagagc aagcggactt ccctgttatc tacgctattg gcattcaaca
      361 acgtgcgggt cttaccccgg accttggacc ggacctccaa cccttgtttg atactattct
      421 caaacacgtc cctgcaccga aagtggatgt ggatgcgcca ttacagatgc tcattaccac
      481 tctcggttat gatgattacc gcggcgtgac ggcggttggg tgtgtctttg ctggcaagat
      541 caaggctggg cagaagctgg cacgcatcaa attagacggt actattttgc ctgaaacggc
      601 tcgctattta tacacattca aaggcttgaa taaagttgat gtagaagaat cgattgcagg
      661 cgatattatt tcccttgctg gtttggaaga tatcgcgatt ggtgaaacct tagcagaccc
      721 caccaaccct attgctttgc cttccatcaa agttgaagaa ccaacagttc gtatgacctt
      781 cggtgtgaat acttcaccgt tctctggtaa agaaggcaaa tggggcacct ctcgcaaact
      841 gcgtgaacgc ttgaatgaag aattgcgcac caatgtttcc ttgcgcgttg cagatacaga
      901 ctcggctgaa aacttcctcg tctctggacg cggtgagttg cacttgggta ttcttattga
      961 aactatgcgc cgtgaaggct atgaatttca ggtctctcgc ccagaagtaa tctatcacaa
     1021 agcagatgac ggtgttttgc ttgaaccgta tgaagaagtt catattgaga ccagcaatga
     1081 aaccgtcgga cccgtagtgg aaatgctcgg tgcaagacgc ggcagaatga tggatatgca
     1141 ggattcaggt cagggaacga cccgtatggt gtacatcgtg ccgacccgcg gtttgctcgg
     1201 cttccgctat caattcctga cgtccactcg tggtgcaggt gtcatgaaca ccattttcca
     1261 tagctatgat gaacttgccg gagctatgaa tactcgtcaa agtggttcgt tggtagcatg
     1321 ggagcctggc acgaccacgg cttatgcatt gaagtctgct gaagaacgcg gcaccttgtt
     1381 cgttggaccg ggcgttgatg tttacgaagg catggtggtg ggcgaaaatg cccgcgccgc
     1441 agatatctct gtcaatgtct gtaaaaagaa gcatttgacg aatatgcgcg cctcacgtgc
     1501 ggatatggaa attcgcctca ctccgccccg acaaatgtct ctcgacgaag cgattgaata
     1561 cctcgctgac gacgaattgc tcgaagtgac gcctgctaca ttccgcattc gcaagcgcat
     1621 tttgaacacc gaagagcgcg gcaagcaaac gaagaaggca aaagaacagt tcggcgaaga
     1681 ataaaaataa aagagccatg caatttgcat ggctcttttt gattcacaaa ctaacagcct
     1741 agtgagattg tctgactttg cgtagaccct gttgcattga aggagataat gctataccca
     1801 gctgtttgac ctgagattag cgtgatggtc tctttaaact ctgttgtatt tgcgggtaac
     1861 tcagcgatca caacatcatt gcgaacgacg cgatagccaa tttcatcatc ttcatctttc
     1921 cacgcaatcg ttactttggc ttcatttgta gcgtaatcac aagcgacatc ccatttttgc
     1981 aagctgacat tttctggtgc tccaccggtg ggtgttggtg gtgccgtaac cgttggaacc
     2041 gcgctaacac tccctgatgg ggtgacgaat tcgccagcta cccaacaagg tcctttgtct
     2101 gtttgaacga tccagtaatt aggcgggtaa tagcccacga tttctaccgc aaaccctggc
     2161 tgaatctggg tgacacgctc gtaatttaca ccgggtccca cacggcaatt ggtgacatct
     2221 tcaaagcttg ccatagggtt tcccgtgctt tctggtgtgg actgtcctgc gctggttggg
     2281 ctggcaagcg gggtttgatt accttcaata gcggcttgaa ctgtcatagc agcggcagtt
     2341 gctacttctg gggaaggggc atcagaacta gacatgctac aagatgtgag gagcaaactg
     2401 gttaggagga ataagtaaac tgatcgtttc attgtattct ccgatgtaaa ttatctttat
     2461 tcgccgtcac catctaacca ggtttccata tacggctttt ccatttcgag agcttgtttg
     2521 gtcaaatgaa gcggatggaa agtatcgacc attacggcga gctctttcgt ctctgtcttt
     2581 ccaatgctgg cttctgtcat accgggttgt ggaccatgcg gcaacccaaa tggatgtaaa
     2641 ctgatatcag accggtctat gcctttgcga gacatgaaat tgccttctgc gtagtagatg
     2701 acttcgtcag attggatgtt ggaatgattg tatggcgcag gaatgccttc ggggtgataa
     2761 tcaaagagac gtggcacaaa agaacataca acgaaattcc agccttcaaa ggtttggtga
     2821 actggcgggg gttgatgtat acgcccggta ataggctcga aatcttcaat gttgaaaatc
     2881 caagggtata agtatccatc ccagccaatg acatcatgag gatgatagtc taggatatgt
     2941 tgcgagatgt ggtcgcgtac tttgacacgc acttcaaact caccacgttc ggtgtgagtt
     3001 tctaactcca tggggatgcg gatatcacgc tcacagtaag gggcatgttc gagcagttga
     3061 ccgtagtgat tgcggtagcg cttgggcggc tcgatctggc tagggttttc gatggtgaag
     3121 aactttgttt cttcgtctaa ttccatttgc caagttgtgc caaatggaat cacgatgtaa
     3181 tcgcctttgc gaaaaggtaa tttgccaaat tgggaatata tggtgccgct accatcatga
     3241 acccaccaag tttcataagc ttgtgagttg cggtagaaaa agttcatttt tcctttagga
     3301 gcccgtgaga ctcccaagat aacatcgtta tttccgagca gaggaattct tccagaaact
     3361 ggatctgccc cgagcggagt gggagccgtt ttgaacgcac gatggcggat cgctccgtaa
     3421 tccacatatt caggtttggc agacccaaga tccgctgtct tttttacgcg tgggggcaga
     3481 aagtgatgat agaggatcga ttgcagcgat gaaaatcctt cgagccccat cagctcttcg
     3541 cgataaagtt ttccatctgg ttttttgaat tgtgtgtgac gtttgtgcgg aattttccca
     3601 agtttgtgat agaacggcat gtcttctcct tatttttcta aaatgacaat tgaagaaatc
     3661 aattcgtcca gcgtgttaag tgaaggagta aattggtcgc cgctggaggt ttctacaagg
     3721 ttattcacat cttgaaaata ggtttcaaac tcttcgggtg ttgtaagagt ttgcccaaca
     3781 tattcactaa gaaaagatgc atcaactagc ataatgaacg acacaaaaaa ctttccatca
     3841 ttcgtaacac cctgatacat gtaggttaac ttcgtgctgc taatggggtc tgctgagaaa
     3901 gactttgcca caagatagcg atagccattg ccattttgga atgccagagg gagcacttgc
     3961 gcatcgataa tgctgcccgc acccgccaag cgcggaaagt caacatggtg atctccttca
     4021 ccttcaagga ccgcattcac actctccacg gctccggttt ccaaagcgtt aacttcatct
     4081 gccagaaaaa cacgcagtcc atttatagtg aaaccatctc caccagaata atcggtgaag
     4141 atgatctgag cattttgagg atatggcagg tcgtaaggag ctaccggcga ataggttgtg
     4201 ttgaagctcg tgctgatgct ttgtgagaag ccttcaggat aattaaaacc gattgcgatg
     4261 cctaactcat taaacgtgga aatattttcc gcctcaaacg cagacggtga aactgtttca
     4321 gtttctgtag gtgggttcgt cgcagatggc tccacagcta gaggtgtcgg gcttgggctt
     4381 tcggttgccg ttgctgtcgg tgcgcaaccg accagaacta ccaatgaaat gcctgtaaca
     4441 agcatatgaa gtttcatttt cacctgcttt ctattctttt acttcatata ctacaacact
     4501 ttcctgctca tttgggtcgt ttctagcgac cacagctatg acttgttctg tatcgctaag
     4561 gttaacgggt tgatgcggta aatttggcgg gatgaaaaga aagtcgcccg cttcgttaat
     4621 tgtggagtgc tcaaggttat gcccatagcg cgtctcggct ttcccttcga tgatgtagat
     4681 cacagtctcg tagccatcat gatagtgagc taccgctttc ccacctggcg ggatgacgac
     4741 aaggtgcatg caaagatgtt tggaccctgc cgtcttcgca gagatgcctt caaaatttgg
     4801 taactgttgt ttgctgaggt gagtcccttg tgggcgaacg gtgatcactg tttcggtcat
     4861 gatgctcctt atttgatttg gatggattga atgagcgcgt ctaaaacgtt taaattcggc
     4921 tgaaatgaat ctggggtagc cgcatttagc aaatcagtga ccccttggaa gtatgcgtcc
     4981 agcgcgatgg tatctgaaat atcgctgggg taaacaacac catcggcaga ggggttttct
     5041 gcagtagcct gtatgggcaa tgtgacgggc accatgattg ctaccatata cttgccatca
     5101 ttggtgagac cttcataatg atagtagctg ccatccttca aaatgggtcc tggaaactga
     5161 gcatactgtg aggtcatgcg gacgccgctc ccattggcaa acgacacgat ttttgcttga
     5221 gccgcatatt gttgagcggc tgcgccattg aaaggaatgg tgggaaggtt atcgtttgtc
     5281 atcgacatgg atgggcttgc caagattgcc tgtagttttg ccaaactacc ttgcgcccat
     5341 gaatttacgg ctgcatattc ctgtgccggg taaatgcgaa tgactggtgg tttgaattca
     5401 tcacgagggg aatagtccat taggacaaat tcaatatatt ctggtgccac gccccagggt
     5461 ccggcagtgg cttcgtctgc ggcagggata acttcactgg atgccccgct tgccaaccct
     5521 tctggaatca caaactgcac attttggaat gacacagaga tgccagtggc ttctggct
//
LOCUS       DAOQFT010000085         5536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7419_length_5536_cov_6.156541, whole genome shotgun sequence.
ACCESSION   DAOQFT010000085 DAOQFT010000000
VERSION     DAOQFT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7419_length_5536_cov_6.156541"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..709)
                     /locus_tag="PLR93_03355"
     CDS             complement(83..709)
                     /locus_tag="PLR93_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26027.1"
                     /translation="MFDLRKRLIAFLFIMAFGMWMAFYQPTQNLVVAQQPTGSIPTVT
                     GTPPGMIVRVNIDQNFEIVPVHSGPNSTIYPVVGVILMGQEIPAVGISEDQDWIQIYY
                     PGVPGSTAWVFGPYVTIIKTGRLPVIPAPNTPTPVSTPTINPTLVAAFVDPVTPTRLP
                     TYTPPPEFVVPTFLPDTGSENRVPVGLFIIALGFIGGFGTLISFLRGR"
     gene            complement(718..1380)
                     /locus_tag="PLR93_03360"
     CDS             complement(718..1380)
                     /locus_tag="PLR93_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH26028.1"
                     /translation="MNSIIIAFAILLSKLYEMTKYKFHPLVLTLTFLLLVVWIFNASF
                     PTNVHAQQPTDTPAPATGLFVTVVTNEPQINVRMGPSATVYPVIGVLLTGSTVPALGR
                     SKGGDWVQIEFASGPNGKGWVYSPLVEVSPAGSTLIVVDAPPTPVQPATSTIDPTLAA
                     QFIIEPTSTHLPTFTPAPTLIKVQFEPSASLSYSIPWASIIIALAVLGVIGFLLSLVI
                     RR"
     gene            1379..1963
                     /locus_tag="PLR93_03365"
     CDS             1379..1963
                     /locus_tag="PLR93_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH26029.1"
                     /translation="MERKIFHGNIKPVDIAQALLAEFNQGNMRAQILGQSDKMVVQVG
                     TRPDAMSGGQTAMTVTIQTLEEGIMVELGQQAWLGVAASLGATALAAIRNPFSLLGRL
                     DDIAQDIENLQISERVWAVISKAARAAGASAELSENLKRTTCEYCHVANPVGQPSCSA
                     CGAPLGNVQPTTCRHCGFVVRPNEKTCPNCQKPV"
     gene            1976..2800
                     /locus_tag="PLR93_03370"
     CDS             1976..2800
                     /locus_tag="PLR93_03370"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HNH26030.1"
                     /translation="MNLREIEARLQTLIEVDLLKVLPGKRVEDVIVLKLATALQQNAI
                     QQDGTTVLPDVYTLILHPETAKAWEDEPFLTVLLNSVATVAQEAGFKFTIPPIITVIR
                     DENIPLNDVEIKTSHRIETMAETNATPLDTGSLEATGEKLPENSFLIVEGVKIYPLNL
                     PVVNIGRRLDNQLVIDDPRVSRNHAQLRAIKGRFVVFDLNSTGGTFVNGQRTSQSVLY
                     PGDVISLAGVALIFGQDNPPPRPDLKDTNPYKMTSDRPTAVFNKPDHSTAEFKKKK"
     gene            2797..3276
                     /locus_tag="PLR93_03375"
     CDS             2797..3276
                     /locus_tag="PLR93_03375"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNH26031.1"
                     /translation="MIGSVVLALRILTALAMYGFLGWVLVFLYREVQRQGIVLANRRV
                     PGISIMVTPKGGAGTLRYFHQAEITIGRDPGCDIPLPEDTISARHAHLTYHHNQWWLE
                     DLASTNGTRLNEMRITTPTVITSGDEIQCGGSNLSVSLSENVIIEPTIRLKNKPDSQ"
     gene            3273..>5536
                     /locus_tag="PLR93_03380"
     CDS             3273..>5536
                     /locus_tag="PLR93_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNH26032.1"
                     /translation="MNNQVQSRLLQWSALFLFLQSIILTLAPAVRERTWDVDYRLSHW
                     FGFLIWCIFTYIAHRTLNHYLPEKDPYIFPAATLLSGWGLLTVWRLDESFGIRQAAWF
                     ATSIIVLILAVRYFRSLSFLRNYKYVFLIGGLLITALTLIFGTNPMGAGPRLWLGLGG
                     VYFQPSEPLKILLVIYLSAYLAERSNIRLSSLPLLIPTLLVTGVALLLLVVQRDLGTA
                     SIFIFLFTIFIFIVTGKRRILIGTGSFLLIALLIGYFLIDIIHVRVVGWLNPWDDPTG
                     NSYQIIQSLLAVANGGTLGRGLGIGSPLLVPVAISDFIFAAIAEETGLFGTLGLIATI
                     WLILARGLTIALRAPDNFRRYLSAGIVAYLGVQSLLIIGGNLRLLPLTGVTLPFVSYG
                     GSSLLTSFIALYLLLSISSVEDDEPAALSNPAPYSILAGIFALGFAACALTSSWWGLV
                     RNADLLDRTDNARRAIADRYVPRGKILDNNNQPINVSTGESGSFIRTYLYPELAPITG
                     YTHPVYGQAGLEANLDDYLRGLQGNPTSLILWNQLIYGTPPAGLDVRLSLDLNLQSTA
                     DALLGKHRGAIILMNAESGEILVMASHPTYDPNKLDEEGDSLSQNAAAPLLNRATQGM
                     YPVGDALLPLLKANFGEATVTDNELLSFYDQLGLFRSPLITLPVAESQPTEKVQDLRA
                     SPLQVVLATAALSNHGVIPAPRIATAVNTLEQGWVVLPALDKPRELFSAEIADEAVHP
                     FVQQGNAYWSSTGQG"
BASE COUNT         1406 a         1350 c         1348 g         1432 t
ORIGIN      
        1 gcggttcttg cagctgcgca aattaatcta agatcgttaa atcaaaagga caggtcatag
       61 acctgtcctt ttttgttcct ttttatcgac cacgtaaaaa tgagatcaag gtgccaaacc
      121 caccaataaa cccgagggca atgatgaata agccaacagg cacacggttt tcggaacctg
      181 tatctggcag gaatgttggg acgacgaatt ctggcggcgg tgtgtaagtt ggtaaacgcg
      241 ttggggtgac ggggtcaaca aaagcagcaa ccagagtagg gttaattgtg ggtgttgaaa
      301 caggcgtcgg cgtatttgga gccggaatga ctggtagtcg tcctgttttg atgatggtga
      361 cgtatggtcc aaaaacccag gcggttgagc cgggtacgcc gggataataa atttgaatcc
      421 agtcttgatc ttcagaaatg cctacggctg gaatctcttg ccccataaga ataaccccaa
      481 caacggggta gatcgttgag ttcggacctg aatgaacagg tacaatctcg aaattctggt
      541 caatgttcac acgtacgatc atgccaggag gtgtaccggt tacggttgga attgaacctg
      601 tgggctgttg tgcaacgaca agattttgag ttggctggta gaacgccatc cacatcccaa
      661 atgccattat aaatagaaag gcgatcaaac gttttctaag gtcgaacatg gtcaatttca
      721 acgacggatc accaaagaaa gtaaaaagcc aatcacaccc aagactgcca atgcaataat
      781 gatcgaagcc cacggaatgc tatagctcaa gctggcagat ggttcaaatt gaactttgat
      841 gagggttggt gcaggggtaa acgtcggcag atgtgtgctg gttggctcaa taatgaattg
      901 cgcggcaaga gtcgggtcaa tagtggatgt ggcaggttgg actggcgttg gcggagcgtc
      961 tacaacaatg agggtgctac ctgctggcga aacctcaacc agcggggaat atacccagcc
     1021 ttttccattg ggtccggatg caaattcgat ctgaacccag tcgccacctt ttgaacgccc
     1081 caaagctggg actgttgagc cggttaataa cacaccaatc acgggataca cggttgcgct
     1141 gggacccatg cgaacattga tctgcggttc attggttaca acggtaacaa agagaccggt
     1201 agcaggggct ggagtatccg tcggttgttg agcatggaca ttagtcggga aagaggcgtt
     1261 gaatatccaa acaaccaata aaagaaaggt taacgtcagt acgaggggat gaaacttata
     1321 ttttgtcatt tcgtaaagtt tagaaaggag aatcgcaaag gcgattataa tcgagttcat
     1381 ggagcgaaaa atctttcacg ggaatatcaa gccggtggat attgcacagg ctttgctggc
     1441 agagttcaat caggggaata tgcgtgcgca gatcttgggt cagagtgaca agatggttgt
     1501 gcaggtgggc acgcgcccag atgctatgtc tggcgggcag accgcgatga ctgtaacgat
     1561 ccaaacactt gaagaaggca tcatggtgga gttgggtcaa caagcttggc ttggagtggc
     1621 tgccagcttg ggtgccactg cgcttgccgc tattcgaaat ccgttcagtc tattagggcg
     1681 cttggacgac attgctcaag acattgaaaa tttacaaatc agcgaacgtg tttgggcggt
     1741 tatctccaag gcggctcgtg cggctggtgc atctgccgaa ctctctgaga atttgaaacg
     1801 cactacttgt gaatactgcc atgttgccaa cccagttggt cagccttcct gcagcgcttg
     1861 tggagctccg cttgggaacg tgcaacccac tacgtgccgt cactgtggtt ttgttgtaag
     1921 acctaatgag aaaacctgcc cgaactgtca gaaacctgta taactaaaaa ccaaaatgaa
     1981 tttacgagaa atcgaagcac gtctccaaac cttgatcgaa gtagaccttt taaaggtttt
     2041 gccgggcaaa cgcgtggaag atgtgattgt cttaaaactc gcaacagcgc ttcaacaaaa
     2101 tgcgatccag caagatggaa cgactgtttt accagatgtc tacacgctca tattgcaccc
     2161 tgaaaccgca aaagcctggg aagatgagcc atttcttaca gttctattaa attctgtggc
     2221 aactgtagcc caagaagccg gatttaagtt tacaataccc cctatcatta ccgtcataag
     2281 ggatgaaaac atccctttaa acgatgtaga gatcaaaaca tctcaccgga tagaaacgat
     2341 ggcagaaaca aacgcaaccc ccctcgatac tggcagcttg gaagcgactg gcgaaaagtt
     2401 acccgaaaat tcattcttaa ttgtagaagg cgtgaagata tatccgctaa atcttcccgt
     2461 ggtcaatatt ggacgccgac tcgataatca acttgtgatt gatgatccgc gtgtctctcg
     2521 caaccatgcc caactccgcg cgattaaggg acgtttcgtt gtattcgacc tcaactccac
     2581 cggcggcacc tttgttaacg gtcaacgcac cagccaaagc gtactctacc cgggcgatgt
     2641 aatctcacta gcaggtgtgg ctctcatttt cggacaagac aatccgccac cgcgcccaga
     2701 cctcaaagat actaaccctt acaaaatgac gtctgaccgt ccaaccgccg tattcaataa
     2761 acccgatcat tccacagctg aattcaagaa gaagaaatga ttggttctgt tgttctggcg
     2821 ctgaggattc tgacagcgct tgcaatgtac ggctttcttg gctgggtgct tgtattcttg
     2881 tatcgtgaag ttcaacgcca gggaattgtg ctggcaaatc ggcgcgtccc cggcattagt
     2941 attatggtga cacccaaagg cggggcggga acgctgcgat actttcatca agccgaaatc
     3001 accattgggc gcgaccccgg ctgtgatatc cccttacctg aagatacaat ttctgcacgg
     3061 catgcacacc tcacctacca tcataatcag tggtggttag aagaccttgc ctccacaaat
     3121 gggacacggc taaatgagat gcgtatcact actccaacgg taatcacctc tggagatgaa
     3181 attcaatgcg ggggttcgaa tttatctgta agcctttctg aaaacgtcat catcgagccg
     3241 acaattcggt taaaaaacaa acctgattcc caatgaataa ccaagtacag agccgtctgc
     3301 tgcaatggtc ggcacttttt ctatttctac aatccatcat cttaacgctt gcgcccgctg
     3361 tgcgtgaacg aacatgggat gtagattatc ggctttccca ttggttcggg tttttgattt
     3421 ggtgtatctt cacttatatt gcccatcgca cacttaatca ttatctccct gaaaaagatc
     3481 cttacatctt cccggcagct acattactaa gcggctgggg tctactaact gtatggcggt
     3541 tagatgaaag ctttggcatt cgtcaggcag cttggtttgc aaccagcatc attgtattaa
     3601 ttctcgccgt tcgttatttt cgaagccttt cattcttaag aaactataaa tatgtgttct
     3661 tgatcggtgg gctgcttatc acagcgctca cactgatctt tggcaccaac cccatgggag
     3721 cgggtccgcg cttgtggctt gggctgggcg gggtatattt tcaaccatcc gagccgttaa
     3781 aaattcttct cgtcatttac ctttctgcat atcttgcaga acgaagtaac atccgcctgt
     3841 cctctttgcc attactgatc cctactttgc ttgtcactgg cgtagctctt ttattattgg
     3901 tggtacaacg cgacctcggc accgcttcta tttttatctt cctcttcacc atttttattt
     3961 ttatcgtaac aggcaaaaga agaatactga tcggcacagg ctctttttta ctaattgctc
     4021 ttctaatcgg ttattttctg atcgatatca ttcacgttcg ggtagtgggc tggctcaacc
     4081 catgggatga ccccaccggc aattcgtatc aaatcattca atctctttta gccgtggcaa
     4141 acggcggcac attgggaaga ggacttggaa ttggaagtcc gctacttgtg ccggtggcga
     4201 tttccgattt tatttttgcc gccatcgcag aagaaacggg tctattcggg accttgggac
     4261 tgatcgccac catctggttg attctcgctc gcggcttgac gattgccttg cgggcgccag
     4321 ataactttcg gcggtattta tcggctggga ttgtggcata cctcggtgtg caaagcctgc
     4381 tgatcattgg tggaaattta cgtctgcttc cgcttacggg ggtgactctg cccttcgttt
     4441 cctatggcgg ctcttcttta ttaacttctt tcattgcctt atatttatta ctgtctatca
     4501 gtagcgttga agatgatgaa cccgcggcgc tttcgaaccc ggctccatat tccatccttg
     4561 caggcatctt cgcccttggc ttcgccgctt gtgctctcac aagctcatgg tggggactcg
     4621 tacgaaatgc tgacttactt gaccgcactg ataacgcccg ccgcgccatt gcagaccgat
     4681 acgttccgcg cggcaaaatt ttagataaca ataatcaacc cattaacgtt tctacgggtg
     4741 aaagcggctc tttcattcgc acctatttgt accctgagct tgcgccaatc acgggatata
     4801 cccaccccgt ctatgggcaa gctggtttgg aagccaacct tgatgattat ctacgcgggc
     4861 tacaaggcaa cccaaccagc ctcatcctct ggaatcaact catttatggc acaccgccag
     4921 ccggattgga tgtgcgttta agcctggacc tcaatttgca atctacggca gatgctttgt
     4981 taggaaaaca tcgaggcgcc atcattctca tgaatgcaga gagcggcgag attctggtca
     5041 tggcatcgca tcctacgtat gaccccaaca aacttgatga agagggagac tccctttcac
     5101 agaacgctgc cgcgccgtta ctgaatcgcg ccacccaagg tatgtaccca gttggagatg
     5161 cacttctgcc gctcttaaag gcaaacttcg gcgaagcaac cgttaccgat aacgagttgc
     5221 tctctttcta cgatcaactc ggactcttcc gttccccatt aattacctta cccgtggcag
     5281 aaagccagcc cactgaaaaa gttcaagatt tgcgtgccag ccccttgcag gttgtattgg
     5341 cgacagccgc cttgagcaac catggggtca tccctgcgcc acgcattgcc acggcagtaa
     5401 acactcttga acagggatgg gtggtgttac cagcgttgga taagccccga gagttattct
     5461 ctgctgaaat tgcggatgag gcggttcatc cttttgttca gcaagggaat gcctattgga
     5521 gcagtaccgg gcaggg
//
LOCUS       DAOQFT010000086         5501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7495_length_5501_cov_6.875689, whole genome shotgun sequence.
ACCESSION   DAOQFT010000086 DAOQFT010000000
VERSION     DAOQFT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7495_length_5501_cov_6.875689"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1130
                     /locus_tag="PLR93_03385"
     CDS             180..1130
                     /locus_tag="PLR93_03385"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH26033.1"
                     /translation="MILITGATGFIGRALVRQLSSIGYPLRALIRPSAKTPRLPKGVP
                     VEVAVVSLADTRGLRAAMRDVETVFHLASAENQGARGSLLITDIQGTQNLVEAAADAG
                     VDRIVYLSHLGAARASGYPAFKAKGIAEESIRAGKVPYTIIRTSLVYGPEDHFTNNLA
                     RLLRISPGVFPVPNGGRVVVQPVWVEDLVTCMLWALQNESTVNQVYEVGGNEFFTLQQ
                     IIEIIMDVTRRRRFLVPLSPITLRAMTVFLESVVPGFPLSSLFLDYFAVNRTTAVDAM
                     PRHFGLMPARFSYRIDYLIYKRWYQRLWSAVTERLSPQRR"
     gene            1132..2010
                     /locus_tag="PLR93_03390"
     CDS             1132..2010
                     /locus_tag="PLR93_03390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26034.1"
                     /translation="MSLPNIRLLETPDEMKLVEDLQRQVWPGSETDVVPSHVLITVVH
                     NGGLVLGAFEEDQLIGFVFGFPGLERTADGPRPKHCSHQMGIHPKYRDSGIGFELKRA
                     QWQMVRHQGLDHITWTFDPLLSRNAHLNISKLGAVCSTYRESEYGELRDGLNAGLPSD
                     RFQVDWWINSRRVEKRLSKRPRPTLKLAHVARSGLRPFYTPQFSDAGLMLAPEHVPPF
                     EDRLLLVEIPTNFMQIKEKDFPLARDWRFFSRELFETAFAKNYIVTDFVFDANEGSPR
                     GMYVLTHGDSTLDEFE"
     gene            complement(2102..2866)
                     /locus_tag="PLR93_03395"
     CDS             complement(2102..2866)
                     /locus_tag="PLR93_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26035.1"
                     /translation="MNRSLNIQLLPLLKKMSLRRGAIFGGLILCALLAFEVFNFGTTS
                     FALRDVLGDLQFAGMYWSSVLAFAFCAIDFAGIARIFTPEQGRDEPAEVWYLFGAWLL
                     AAAFNAMLTWWGVSVAIRNHNALGGGLVGQETMMTVVPIFVACMVWLIRVLLIGTFSV
                     AGERLFSMADIYDRNTAYRNVPASYSTPQPRTVQPAPRQEVNPPVNNYPRPVPKPQPR
                     PNPMPAYGANRPEPTYHPVGMSAMSRNENSNPSVRR"
     gene            complement(3040..3723)
                     /locus_tag="PLR93_03400"
     CDS             complement(3040..3723)
                     /locus_tag="PLR93_03400"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNH26036.1"
                     /translation="MSPTYLMYMIPAFILMALTSWYVKSAYAKWSRVAAGSRFSGYEA
                     TRRLIASSGLQGVKIQGTPGEMTDHYDPRDKTVYLSNGVANSASVAALAIAAHELGHA
                     QQDAEEYFPLKFRGVLVPLVNIGSNLGWILIMIGLFLQLTQLAWLGILVFSGGALFAL
                     VTLPVEFDASARARRMLADSGIIQTDAEMRGVSNVLNAAALTYVAGLVTAVLQLLYYV
                     SLVSGRSRD"
     gene            complement(3759..5027)
                     /locus_tag="PLR93_03405"
     CDS             complement(3759..5027)
                     /locus_tag="PLR93_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNH26037.1"
                     /translation="MNKTLKAVLLILAVAVVGLGSFAGGFITGHLLPFNNSSAFSFGE
                     APTAEPAPTASPEQQSSTPEDMQTLFAPFWEAWNLVHQNYVDQPVDDVALMRGAISGM
                     MQALGDQHSSYMDPEDFKQANQSLEGSYEGIGAYVDTTSDYLTITSPIPGSPAEEAGL
                     LPGDKIVAIDGEDMTGIDGELVRQKVLGPAGSTVVLTIMRDGEDKPLDFSIVRAQITI
                     ASATGKMLDGDIAYFQVTTFGSNTMPELEAALAEVMPQKPKAIILDLRNNGGGYLQTA
                     VEVASQFMGEGVVLYEQYGDGTRNTFEVIPGGKATDTSIPMIVLINEGSASASEIVAG
                     ALQDTGRAKLLGTVSYGKGSVQNWIPLSNDNGAVRITIAKWLTPNERTIHKIGLTPDY
                     PVDLTPEDRQADKDPQLDEAVRLLLEMLAK"
     gene            complement(5129..5365)
                     /locus_tag="PLR93_03410"
     CDS             complement(5129..5365)
                     /locus_tag="PLR93_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26038.1"
                     /translation="MEMSKTAIAALIFILMIVGVNMVMYGIVRGVMRGGKDDPLLKMM
                     KAMNPAQQKKENEMEELRRTVDELNKGKTGGDSE"
BASE COUNT         1309 a         1358 c         1471 g         1363 t
ORIGIN      
        1 ttggtttctt caccatgcgc aagaagcctg aaactgctgc ggctcccgtc agcaacgatc
       61 agactcctcc tgctgcgtaa attttttcag aagattcaga aaacaggcat tcgctcgagt
      121 gcctgttttt gttttgtaga ctttttcgcg ggatgtaatc tgcctgtata atccaagcca
      181 tgattctcat cactggcgcc accggattca ttggacgcgc cctcgtacgc caactgtcat
      241 ctatcggata ccccctccgt gctcttattc gtccttctgc caagacgcca cgcctgccca
      301 aaggcgtgcc tgttgaggtg gcggtggttt cgcttgcaga tacgcgcggt ctgcgtgctg
      361 ccatgcgcga cgtggaaacg gtctttcact tagccagcgc tgaaaaccaa ggggctcgcg
      421 gaagtttgct catcaccgat attcaaggga cacaaaacct cgttgaagcc gctgccgatg
      481 ccggcgtgga ccgtatcgtc tatcttagtc atctaggggc ggcgcgtgca tcggggtacc
      541 ctgccttcaa agccaaaggg attgccgaag aatccattcg cgcgggcaaa gtgccatata
      601 ccattatccg tacttcattg gtctatggac ctgaagatca cttcaccaac aatttagcgc
      661 gcttgcttcg gatttcaccg ggagttttcc ccgtccccaa tggcggacga gtggtggtgc
      721 agcccgtatg ggtagaagac cttgtcactt gtatgctctg ggcgttgcaa aatgaaagta
      781 cggtcaatca ggtatacgaa gtaggtggca acgaattttt taccctgcaa cagatcattg
      841 aaattatcat ggatgttaca aggcgacgac gttttcttgt accactttcg ccgatcacct
      901 tgcgtgccat gacggtgttt ctcgaaagtg tggtgccggg ctttccactt tcttcgctct
      961 ttcttgatta tttcgccgtc aatcgcacca cggcagttga tgcgatgccg cgtcactttg
     1021 gtttgatgcc tgctcgtttc tcgtaccgta ttgattactt gatctacaaa cgttggtacc
     1081 aacgcttatg gagcgcggtc accgaacgat tatccccaca gagaagataa catgtccctt
     1141 cccaatattc gcctgcttga aactcccgat gaaatgaaat tggttgaaga cctgcaacgg
     1201 caggtttggc ccggctctga gacagacgtc gtcccaagcc atgtgttgat caccgttgtc
     1261 cataatggcg gtttggtgct gggtgctttc gaagaggacc aattgattgg ctttgtcttt
     1321 ggttttcctg ggctcgaacg cacggcagat ggtccgcgcc ctaagcattg ttcgcatcaa
     1381 atgggcattc atccgaaata tcgcgatagt ggcattggct ttgaactgaa acgtgctcaa
     1441 tggcaaatgg ttcggcatca gggtctcgac cacattacat ggaccttcga cccgttgttg
     1501 agccgcaacg cgcacctcaa catttccaag cttggcgcag tgtgcagcac ttatcgtgaa
     1561 tctgaatatg gtgagttgcg tgatggacta aacgccgggc tgccttcaga ccgttttcaa
     1621 gtggattggt ggatcaattc gcgccgtgta gaaaagcgac tcagcaaacg tccacgcccg
     1681 actctcaaac tggctcatgt tgcccgcagc ggactccgcc ccttttacac gcctcaattc
     1741 tccgacgcag gcttgatgct tgcacccgag catgtgccac cctttgagga tcggctcttg
     1801 ttggtggaga ttcccaccaa ttttatgcag atcaaagaaa aagactttcc tctggcaaga
     1861 gattggcgct ttttcagccg tgaactgttt gaaactgcgt ttgccaaaaa ttacatcgtt
     1921 actgattttg tctttgatgc caatgaaggc agtccgcgcg ggatgtatgt tctaacgcat
     1981 ggtgatagta cgttggatga gtttgaataa atttgttctt aacgcatgag acgccctttc
     2041 ggacgtctca gcggttggat ggggaagagg atttcgagaa accttatctc acagacaagg
     2101 actatctcct tacagaggga ttgctgttct cgttgcggct catggcagac atgcccacag
     2161 gatgataggt tggttcggga cgattggcgc cataagcagg cattgggttt ggacggggct
     2221 gcggcttcgg aacgggtcgc gggtaattgt ttacgggtgg attcacttcc tggcgtggag
     2281 caggctgaac ggtccggggt tgaggcgtgg agtaggaagc aggaacgttg cggtatgccg
     2341 tgttgcggtc ataaatatcc gccattgaaa agaggcgttc tccagcaaca gagaaggttc
     2401 cgatcaacag cacgcggatg agccaaacca tgcaggcaac gaagatgggg acaacggtca
     2461 tcatggtttc ttgaccgacc aaaccaccgc ctaaggcgtt atggtttcga atggcaaccg
     2521 aaacacccca ccaggtgagc atggcattga aagcagccgc gagcagccac gcgccaaaga
     2581 gataccaaac ttcggcgggt tcatcgcgac cctgttcggg ggtaaagata cgagcgatac
     2641 cggcgaagtc gatggcgcaa aaggcaaacg ccaaaacaga agaccaatac atccccgcaa
     2701 attgtagatc accgagaaca tcgcgcaagg caaatgaagt ggtgccaaag ttgaacactt
     2761 caaaagcgag gagtgcgcac aagatcagcc cgccaaagat cgcgccgcgt cttagcgaca
     2821 ttttcttaag caagggcaga agttggatat tgagggaacg gttcatggga attctccttt
     2881 ttggaaccaa aaacgggtca agttatctga ggttaaatat agaacacttg ttctaatttg
     2941 tcaagttaac gttggattgc aggagaattg catgaatgga tatcccccaa aaaaatatcg
     3001 gggcggcttg caagccgccc cgattgatat tgcataaact taatcgcggc tgcgtccgct
     3061 gacgagtgag acgtaataca gcagctgtaa aacggcggtg accaaacctg ccacataggt
     3121 caatgcggcg gcgtttagaa cattgctaac accacgcatt tcggcgtcag tttggataat
     3181 gccgctgtcg gctaacatgc gtcgggcgcg ggcagaggcg tcgaactcca cgggcaaggt
     3241 gacgagtgca aaaagggcac cgcctgagaa gaccaaaatc ccgagccatg cgagctgtgt
     3301 caattgcagg aacaagccga tcatgatgag gatccagccg agattggagc cgatattgac
     3361 aagcggtacc agcacaccgc ggaacttgag cggaaaatat tcttctgcat cttgctgggc
     3421 atgaccaagc tcatgtgcgg caattgccaa agccgctaca gaagcactgt tcgcgacgcc
     3481 attcgaaaga tacacggtct tatcgcgcgg gtcgtagtgg tcggtcattt cgccgggagt
     3541 gccttggatt ttcacgcctt gcaaaccgct ggaggcgatt aggcgacgtg tggcttcgta
     3601 cccgctgaag cggctgcctg ctgcgactcg gctccatttt gcatatgctg atttgacata
     3661 ccatgatgta agcgccatca aaatgaaggc ggggatcatg tacattaagt aagtgggact
     3721 caagaaaaac attcagttct cctttctagt tgattaagct atttggcgag catctccaag
     3781 aggaggcgta cagcttcatc caattgtggg tctttatctg cctgacggtc ttcgggtgtc
     3841 aggtctacag ggtaatctgg ggtaagccca attttatgga tggtacgttc attcggagtg
     3901 agccattttg cgatggtaat gcggacggct ccattgtcat ttgaaagcgg aatccagttt
     3961 tgtacggagc cttttccata ggaaacggtt ccaagcaact tggcgcgacc ggtatcttgc
     4021 aatgcgccag ctacaatctc agaagccgaa gcagaccctt cgttgatcaa tacgatcatg
     4081 gggatgctgg tatcggtcgc tttgccgcca gggatcactt caaaggtgtt gcgagtgcca
     4141 tctccatatt gttcgtagag cacaacgcct tcgcccataa attgcgaggc aacttccacg
     4201 gcggtttgta aataaccacc accattgtta cgcaggtcga gaatgatagc tttcggtttc
     4261 tgcggcataa cttcagcgag ggcagcttcc aactcgggca tggtgtttga gccgaaggtc
     4321 gtcacttgga agtaagcgat atcaccatcg agcattttgc cagtggcaga ggcaatggtg
     4381 atctgggcgc ggacgatgga gaagtctagc ggtttgtctt cgccatcacg cataatcgtc
     4441 agcaccacgg tggaaccggc aggtccgagc actttctggc ggacgagttc cccgtcgata
     4501 ccggtcatat cttcgccatc aatggcaacg atcttatcgc cgggtaataa acctgcttct
     4561 tcagccggag agccgggaat ggggctggtg atggtcaaat aatcactggt tgtatccaca
     4621 taggcgccaa tgccttcata ggaaccttcc aagctttgat tggcttgctt gaaatcttca
     4681 gggtccatgt aggacgaatg ttggtcgccc aatgcctgca tcatgccgct gatggcaccg
     4741 cgcatgagag caacatcgtc tacgggttgg tctacataat tttggtggac gaggttccac
     4801 gcttcccaga agggagcgaa gagagtttgc atatcttcag gggttgaaga ctgttgttct
     4861 ggcgatgccg tgggggcagg ctctgcggtg ggagcttcgc caaagctgaa ggctgaactg
     4921 ttgttaaaag gcaaaaggtg cccggtaata aagccaccgg caaaggaccc aagcccaacg
     4981 acggcaacag ccaaaataag cagtactgct tttaaagttt tattcacaag atcctccaat
     5041 agctagcata cgtagaaaga taccaattgg ttattaaaga ttgctgaatg aattattcga
     5101 tttgcgtaag aatcgaacgg ttaacctttt actcagaatc cccgcctgtt ttgcccttgt
     5161 tcaattcatc gactgtgcgg cgtaattctt ccatttcgtt ctcttttttc tgctgggcgg
     5221 ggttcatggc tttcatcatt ttgagcaggg ggtcgtcttt gccgccacgc atcaccccgc
     5281 ggacaatgcc atacatgacc atattaaccc ctacgatcat gaggataaag atcaaagcgg
     5341 caatggcagt tttactcatt tccatggtga ctctccaaaa tggggatgaa ctggttccag
     5401 tctgtgagtt gcgcatcggc gctgccagcg gggaactctt cgttatatag aatactgaac
     5461 aagcccgctt ctttcgcggc gcgggtggtg cgatggatgt c
//
LOCUS       DAOQFT010000087         5483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7530_length_5483_cov_6.507185, whole genome shotgun sequence.
ACCESSION   DAOQFT010000087 DAOQFT010000000
VERSION     DAOQFT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7530_length_5483_cov_6.507185"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLR93_03415"
     CDS             complement(<1..229)
                     /locus_tag="PLR93_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNH26039.1"
                     /translation="MEEQKTREESTNVNNRSVDDLGLTPRATEGLKRAGILNIGQLVE
                     KFAEGDAALLAINGFGQSALTAAKKKLRAMDI"
     gene            complement(239..442)
                     /gene="rpmE"
                     /locus_tag="PLR93_03420"
                     /pseudo
     CDS             complement(239..442)
                     /gene="rpmE"
                     /locus_tag="PLR93_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            complement(442..702)
                     /gene="rpmA"
                     /locus_tag="PLR93_03425"
     CDS             complement(442..702)
                     /gene="rpmA"
                     /locus_tag="PLR93_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNH26040.1"
                     /translation="MAHKVGGGSSRNGRDSNSQRLGVKRYAGQFVLSGNVLVRQRGTR
                     IKPGLNVMVGKDDTLFATADGVVMFDVYRGKKRVNIVPAEVE"
     gene            complement(723..1031)
                     /gene="rplU"
                     /locus_tag="PLR93_03430"
     CDS             complement(723..1031)
                     /gene="rplU"
                     /locus_tag="PLR93_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNH26041.1"
                     /translation="MKFAIVESGGKQYRAVEGTTIDVDRLALELGTKFDFERVLLVAD
                     GDNVSVGTPTLSVKVSATVVDHVKGPKTLAFKYRPKKRIRVRGGNRAHYTRLKIESIG
                     "
     gene            complement(1281..1757)
                     /locus_tag="PLR93_03435"
     CDS             complement(1281..1757)
                     /locus_tag="PLR93_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26042.1"
                     /translation="MPTPIKPAVKPGSAIAKPGTVSYVKFSDKLTASVTDIAKIIEQH
                     KEMIDSIQEVAIELTSSIGSLHALTVKYARTANQILDVLLPILKNLPIIPKNVMQLLV
                     NLESLTQKIIDNEKVTAKTITDVNSGLKTGDANKLKAHAGQLKEVTKTITAIIPKA"
     gene            1906..2751
                     /locus_tag="PLR93_03440"
     CDS             1906..2751
                     /locus_tag="PLR93_03440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNH26043.1"
                     /translation="MSDADVRWERIRNILLLAVLVIIGLLIGSAIFPQPVIGVIRLED
                     AIYTYSAQNTIKQIQYAIDHSEVKAVVFVIDSPGGTVVDTESIYMELTRLREKKPVVT
                     VVNGMSASGGYYLSANTDYIFAKPTSLVGNIGVIGYLPPAPFVIEDVITTGPYKLWGA
                     PRDSDMRQVEMIKQGFFDAVRFGRGDKLTAEENVTFSGQIWAGIDALKLGIIDEFGTE
                     SDGAKKAAELAKVANYEIVDLAAPAGIDMSGGFFFMASADGVTLPYPKEAGMYLLYIP
                     PLPVK"
     gene            2764..>3080
                     /locus_tag="PLR93_03445"
     CDS             2764..>3080
                     /locus_tag="PLR93_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26044.1"
                     /translation="MKRQTIYIALAALILPILLRGLWFYRGLPTQRPEIATPDYASFE
                     RPAVSVSTPDLENVKRLGGSVLIDAAHTNQFTMTEIDSFIEAIRVRGGTVKTITDATM
                     LET"
     assembly_gap    3081..3180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3181..4373
                     /locus_tag="PLR93_03450"
     CDS             <3181..4373
                     /locus_tag="PLR93_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26045.1"
                     /translation="VRGGTVKTITDATMLETELKTSSAYITVSPSSAFAESEVQALKK
                     FADRGGKILAFAEPTRYFLGFDYVSGNPIPYGDVSAVNSLLKSWDISVNNDYLYNTQK
                     NEGNFRNVLFEDFGKSELTFGLNEVALYGSRSVETDSGLILLQGSETNLSSMDDAHHP
                     HTGGAALSEDGSVAVFGDFTFMASPYNTYTDNATLIQNMADFALAGQQQQSLDVFPYL
                     FKQTTVKVYVSSEIEKTSTMVSALGSLQSTMAALNYKLEFVDSVPTSGDAILVGTFDA
                     SDEFDTYLKKSDVEIDADLLSTVAFGELSSSGNGLLLFNSNKSGNTLVLLAGSSDDVI
                     SLLSVMGYGGLSSCLTSEQVAVCSVGSGDYYYDDTSSDVSTDGTTTDSSTEPTPEATS
                     TPAG"
     gene            4382..5053
                     /locus_tag="PLR93_03455"
     CDS             4382..5053
                     /locus_tag="PLR93_03455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNH26046.1"
                     /translation="MSELIQLGEVKGHFAKPEGEGPFPALIVIQEWWGLDAQTKSIAD
                     RFAKEGYLAFAPDLYHGELAQLGDGDTAMKLVQKYGPTAAPQLATVFDALKARPECNG
                     HVGSVGFCFGGRMSLALSTMRSVDAVCTFYGGGMQTIFDQLREKLKAPVLGLFGDADV
                     SIPAGTVEEFDKLLDDVGVEHQVIMYPNSGHAFFRDSDPNVYKPEASKDAWERVKAFF
                     AKNLN"
     gene            5135..>5483
                     /locus_tag="PLR93_03460"
     CDS             5135..>5483
                     /locus_tag="PLR93_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26047.1"
                     /translation="MPNLSLLQRQIIESPLNARLFVSGVTGTGKTTAGVERIRFLLDQ
                     GIPADTILLLTPQRTLQEPYLDLLESPNRMAGGEVTPATIGGLARRMCDLFWSLTAEE
                     AGFHNPDQPPIFLT"
BASE COUNT         1338 a         1269 c         1379 g         1397 t
ORIGIN      
        1 taatgtccat tgcgcggagc tttttcttgg cagccgtcaa agcagactga ccaaaaccat
       61 tgatcgcgag aagagcggca tcgccttcag cgaatttttc aacaagctga ccaatgttca
      121 aaatgccagc gcgcttgaga ccttcggtag cacgcggggt gagaccgaga tcatcgacag
      181 agcggttgtt gacattggtg gattcttcgc gggtcttctg ttcttccaca taggactggc
      241 gagcagaaag cttcttatag aagcggtcta cctgaccttc gacatcgagg atcttagcag
      301 attcaccggt gtagaaagga tggcaattgg agcacacgtc cacgcgtaat tctttcttgg
      361 tggaagtagt aatccaagtg ttgccgcagg aagcacaagt gaccttggca tcgtacactg
      421 taggatgaat atccgctctc attattccac ctcagccgga acgatgttca cgcgcttctt
      481 gccgcgatag acatcgaaca tcaccacacc gtcggcagtg gcgaacaaag tatcatcttt
      541 accgaccatc acgttcaaac ccggcttgat gcgggttccg cgctggcgca ccaacacgtt
      601 gccggaaagc acaaactgac cggcgtagcg cttaacaccc aagcgctggg aattgctatc
      661 tctaccgttg cggcttgaac caccgcctac cttatgagcc attgtctacc tacctttatt
      721 tcttaaccga tggattcgat cttcaaacgg gtgtaatggg cgcgattgcc gccacgcaca
      781 cggatgcgtt tcttcgggcg atacttgaat gcgagggtct tgggtccctt aacatggtcc
      841 accacagtgg cagaaacttt cacactgagg gttggtgtac ccacggagac gttgtctcca
      901 tcagccacga gcaaaacacg ctcgaagtca aactttgtgc ccaattcaag ggcgaggcga
      961 tccacatcga ttgtggtgcc ttcgacggca cgatattgct tgccgccgct ttcaacgatt
     1021 gcaaatttca ttttctattt tctccagtca ttcacttcgc cgagaaagtt cgcggggatc
     1081 ttgcaccctt cgcctacttc tggggaagct ggggttgtaa acaaacaact accccggaat
     1141 ctgacctccg gggtggaagc ggacggtatt atacatgcag agtctgggac ttgtcaacga
     1201 gatttgggcg agggagaact gccaaggcgg ggtgtcctgc cccgttgggc gccgcaggtt
     1261 taggtccaga aagaggcggg ctaggctttg gggataatag ccgtgatggt cttggtcact
     1321 tccttcaatt gacccgcatg cgccttcaat ttattggcat cgcctgtttt caagccagag
     1381 ttgacatcgg taatggtctt ggcggtgacc ttttcattgt cgatgatctt ttgggtcagg
     1441 ctttcaaggt tgaccaacaa ctgcatcaca ttcttgggga tgatgggcag gtttttgaga
     1501 atagggagca acacatcgag aatctgattc gcggtgcggg catatttgac ggtgagagca
     1561 tgcagggagc cgatggaaga ggtcaattcg atggcaactt cttgaatcga gtcgatcatt
     1621 tctttgtgtt gttcaatgat ctttgcaatg tctgtgaccg aggcggttaa tttatctgaa
     1681 aacttgacat aggatacggt gcccggtttt gcaatggcgc ttccaggttt tactgcaggt
     1741 ttgattgggg ttggcatgag aaaatacctc ctgaaaaaat ttgtgctgca catgcagcaa
     1801 gatatgccaa atatactgta tatcgtttcg caaaacaacc ttgttgacaa agcatgtttt
     1861 ctcgtatatc ctgaaagcga attccaactt atagaggaga aacccatgag cgatgcggat
     1921 gtacgttggg aacgaattcg gaacatactg ctattggcag tccttgtaat catagggttg
     1981 ctaataggct cggcaatctt ccctcaacca gtcattgggg tgatacggct ggaagatgcg
     2041 atttatacct attcagcaca aaacactatc aaacagatcc aatacgcgat tgaccattca
     2101 gaagtaaagg cggttgtatt tgtgatcgat agccccggtg ggacggtggt ggatacagag
     2161 tccatttata tggaactgac ccgtttgcgc gaaaagaagc cggtggtgac agtggtcaat
     2221 gggatgtctg ccagcggtgg gtattatctt tctgctaata cagactatat cttcgccaag
     2281 cctacgtcat tggttgggaa tattggtgtg atcggctact tgccccctgc cccatttgtg
     2341 attgaagatg tcattacgac gggaccgtat aagttatggg gtgcgccgcg tgattcggat
     2401 atgcgtcagg tggagatgat taagcaaggc ttctttgatg cggtccgctt tgggcgggga
     2461 gataagctga cagcggaaga aaatgtcacg tttagcggac agatctgggc ggggatcgat
     2521 gcgctcaagt tgggcatcat tgacgaattt gggacggaga gcgatggcgc caagaaagcc
     2581 gcggaattgg caaaagtcgc caactatgaa attgtagatt tggctgctcc ggctggtatt
     2641 gatatgtctg gcggcttttt cttcatggca agtgccgacg gcgtgacatt gccgtatcca
     2701 aaggaagcgg gcatgtatct tctttatatt ccgcctcttc cggttaaata ataggaggat
     2761 gaaatgaaac gccaaactat ttatattgcg ctcgctgcct taatcctccc cattctcttg
     2821 cgtggactat ggttttaccg tgggcttcct acacagcgac ctgaaattgc tacgccagat
     2881 tatgcatctt ttgaaagacc ggctgtctct gtgagtacgc ctgacttgga aaatgtgaag
     2941 cgacttggtg gcagtgtttt gatcgatgct gcgcatacga atcaatttac gatgaccgaa
     3001 attgactcgt tcatagaagc gatccgcgtg cgtggaggga cggtcaaaac gatcaccgat
     3061 gcgaccatgc tggaaactga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 gcgtgcgtgg agggacggtc aaaacgatca ccgatgcgac catgctggaa actgagctaa
     3241 agacgtcaag cgcgtacata accgtctcac catcttcggc atttgctgaa agcgaagtgc
     3301 aggcactcaa aaagtttgca gaccgcggcg ggaagattct ggctttcgca gagccgacgc
     3361 gctattttct tggtttcgac tatgtctctg gaaacccaat cccatatgga gatgtaagtg
     3421 ccgtaaattc cctgctaaag agttgggata tttctgttaa taatgattat ctctataaca
     3481 cccaaaagaa tgaaggaaac ttccgcaatg tgctgtttga agattttggg aaaagtgaac
     3541 taaccttcgg cttgaacgaa gtggcgttat atggttcgcg ctcggttgaa acggattcag
     3601 ggttgattct gcttcaaggg tcagagacca atctctcgtc gatggatgat gcgcatcatc
     3661 cacacacggg cggagctgca ttgagcgaag atggaagtgt agccgtcttt ggcgacttta
     3721 ctttcatggc atctccatat aatacgtata ccgataacgc gactctcatt caaaacatgg
     3781 cagactttgc acttgcaggt cagcaacaac aaagtttaga tgttttcccc tatctcttca
     3841 agcaaacgac cgtgaaggtg tatgtctcat ctgagatcga aaagacttct accatggttt
     3901 cagctttggg aagtttgcaa tcaacaatgg cggctttgaa ctacaaactt gaatttgtag
     3961 attcggtccc gacctctggt gacgctattt tggttggaac attcgatgcg tccgatgagt
     4021 tcgataccta cttgaagaag tcggatgttg aaattgacgc cgaccttctc agcaccgttg
     4081 catttggaga attaagcagt tctggaaatg gtttgctgct gttcaactcc aacaagagcg
     4141 gaaatacgct tgttcttttg gctggctcgt cagacgatgt catttcttta ttaagtgtta
     4201 tgggctatgg cggcttaagt tcgtgcctta ccagtgaaca agtagccgta tgcagtgttg
     4261 gcagcggtga ttattattac gacgatacga gttctgatgt atctaccgat ggaacgacaa
     4321 cagatagttc cactgaaccc actccagaag caacatcaac tccagcaggg tagaggtatc
     4381 tatgagcgaa ttaattcaac taggtgaagt aaaaggacat tttgcaaaac cagaaggtga
     4441 aggtcccttc ccagcattga tcgtcattca agaatggtgg ggcttagatg cccaaaccaa
     4501 atcaattgca gaccgctttg ccaaagaagg gtatctcgcg tttgcgccag acctttatca
     4561 cggcgaactt gcccaactcg gcgatggcga taccgccatg aaactcgtgc agaagtatgg
     4621 tccaactgct gcgccgcaat tagcaactgt attcgacgct ctcaaagcac gccccgaatg
     4681 taacggacat gttggtagtg tgggtttctg tttcggcggt cgcatgtcgc tggcgctgag
     4741 cacaatgcgc tccgtagatg ccgtttgtac tttctatggc ggcggtatgc aaaccatctt
     4801 cgaccaactg cgtgaaaaat taaaagcccc tgtgttgggt ctctttggcg atgcagatgt
     4861 ttccattcca gccggtaccg tggaagagtt cgataaactt ctcgatgatg tgggcgtgga
     4921 acatcaggtc atcatgtatc ccaattcggg tcatgctttc ttccgtgata gcgacccgaa
     4981 cgtctataaa cccgaagcct caaaagatgc ctgggaacga gtcaaagcat tctttgcaaa
     5041 gaatttgaat taagaaaaga agacgagagg accgcaggca agtgtctgcg gtcttttcat
     5101 tgacaaaacc tccgcgatac tttacattcg cttcatgccc aaccttagcc tgcttcagcg
     5161 tcagatcatt gaatcccctc tgaatgcacg tctcttcgtc tctggcgtaa cgggcacagg
     5221 caaaaccacc gcaggcgttg aacggattcg cttcttgctc gatcaaggca ttccagccga
     5281 taccattctg ctcctaactc cacagcggac tcttcaagaa ccatatcttg acttgcttga
     5341 aagcccaaac cgaatggcag gcggtgaagt aacacctgcc accatcggcg gtcttgcacg
     5401 ccgcatgtgc gacttgttct ggtcactcac tgcagaagaa gctggctttc acaacccaga
     5461 ccagcccccc atcttcctta cat
//
LOCUS       DAOQFT010000088         5479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7536_length_5479_cov_6.174594, whole genome shotgun sequence.
ACCESSION   DAOQFT010000088 DAOQFT010000000
VERSION     DAOQFT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7536_length_5479_cov_6.174594"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLR93_03465"
     CDS             complement(<1..383)
                     /locus_tag="PLR93_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HNH26048.1"
                     /translation="MARQKYYVVWKGRKTGIFTSWAECEKQVKGVVGAQFKAFESEAE
                     ADAAYLAKYEDYIGKVSTSGRWREASIKPLLPSVCVDAACSGAPGTLEYRGVNTETGE
                     EIFHSGPYADGTNNVGEFLAIVHALT"
     gene            672..1511
                     /locus_tag="PLR93_03470"
     CDS             672..1511
                     /locus_tag="PLR93_03470"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNH26049.1"
                     /translation="MSYAHPEYLVETDWVAEHLNDPNIRIIESDEDPLLYAIGHIPGA
                     AQVDWFSTLQHPLRRDFLTKEQFEQVASKLGITNDTTVIFYGDKSNWFACYALWLFQY
                     YGHQNVKIVNGGRAKWEKEGRPLVKEVPTFTATTYKAKEADKSIRAFRDDVFKQVDSK
                     KPLVDVRSPKEYTGELLHMPNYPQEGATRGGHIPGAVSIPWAQAVNEADSTFKTAEEL
                     KSLYEGKNIKSDGEIIAYCRIGERSSLTWFVLKYLLGYPTVKNYDGSWTEWGNLVDAP
                     IEK"
     gene            1575..2006
                     /locus_tag="PLR93_03475"
     CDS             1575..2006
                     /locus_tag="PLR93_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNH26050.1"
                     /translation="MTLPNKLQEIVDDFASMSREEKLETLIAYAESMPELPERFHAER
                     EKMEAVPECMTPVFIAAEKDSNNGIVFHLDIPKQSPTVRGLASILTNGLNGCSLAEII
                     SVPAEFYLPMNLQEAVSQQRINGFMGVLAHMKQVAVRVGSG"
     gene            complement(2056..2460)
                     /locus_tag="PLR93_03480"
     CDS             complement(2056..2460)
                     /locus_tag="PLR93_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH26051.1"
                     /translation="MNNLVRDWMSSPVVVVDPDSSVSHAMTLMRRRNIHSVVVDVSEK
                     NPSYGIVTTTDVRDKIVVAGRNPAETTVREIMSGPIVTGRAEWTLAECSRLMQERKIR
                     HLPIADEHGTLIGLISATDIFMAVEEQGWIEG"
     gene            complement(2572..3765)
                     /locus_tag="PLR93_03485"
     CDS             complement(2572..3765)
                     /locus_tag="PLR93_03485"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26052.1"
                     /translation="MKRFLSFLFILTALVSACAPAVATTAAPEASEPAATGTEEPQAL
                     SQAVLNTPLPAVAEAPIIESPAILNIQMLDEVNGWALTEEKVVRTNDGGVTWYDVTPK
                     NLKDAGYLVNTDFLDENTAWVQFPDMNKYPNGGTLYHTTNGGLTWESFSTPFSGGYIQ
                     FVDANNGWMLSDLGVGAGSMAVSVFQTSDAGKTWNRTYTNDPNLADASDTLPLGGIKN
                     LILPLNAKTAWIGGVVYAPGETYLFRSDDNGKTWVNINLILPQEAAGSELSVIGMVFL
                     SPKTGLLALRVATETPKTIVYVTQDGGNTWTQSEVSFEGYGLLETPSANEMVFYVNDQ
                     FYVTKDAGVTVQEIIPDVKFGDAIMDMSFANAQTGWIVASDASGNYTLYKTTDSGSTW
                     TVLSQ"
     gene            complement(3790..4998)
                     /locus_tag="PLR93_03490"
     CDS             complement(3790..4998)
                     /locus_tag="PLR93_03490"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNH26053.1"
                     /translation="MSTPVIVDAIRTPIGALGGILSSVRPDDMAAHVIKSILERNQID
                     PAQIEEVFMGCANQAGEDNRNVARMAALLAGLPVEVGGVTVNRLCASGLNAINMASRA
                     IKAGEGDVYIAGGVESMSRAPYSLPKAEAGFSFGNLTAYDTALGWRYPNPKMKAMHGT
                     DSMGETAENIAAERPHITREKQDAFAMRSHQRAIAAIDSGRFKQEIVPVTIPQKKGDP
                     KVVDTDERPRRDTTMESLGKLKAAFRDGGTVTAGNSSGLNDGASALLIMSEEKAKALN
                     LKPLARIVTSASAGVPPRVMGIGPVPATKKAVERAGLKMQDIGLMELNEAFAVQSLAV
                     IEDLGIDPELVNVNGGAIAIGHPLGCSGARLMTTLVHEMKNRGNVKYAVATLCVGVGQ
                     GEATVIEYLG"
     gene            complement(5058..>5479)
                     /locus_tag="PLR93_03495"
     CDS             complement(5058..>5479)
                     /locus_tag="PLR93_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH26054.1"
                     /translation="PGFSLWQVSSMWQRQINAGLKPFDLTHAQFVLLASLMWLAGEDK
                     PLTQVDLANHAKMDVMMVSNVLRTLEDKGFVTRKPHPTDTRAKALSITGQGRKLATQT
                     IGVVEKIDHDFFNSLGTDVKNFNKHLQALIITNRSTD"
BASE COUNT         1412 a         1448 c         1248 g         1371 t
ORIGIN      
        1 gtcaatgcat ggacaatcgc caaaaactcc ccaacattat tcgtcccatc ggcatatgga
       61 cccgaatgaa agatctcttc gcctgtttca gtatttacac cgcgatattc caacgtgccg
      121 ggcgccccac tgcatgcggc atccacacaa acagacggca acaaaggttt gatgcttgcc
      181 tcacgccagc gtccactggt agataccttc ccaatatagt cttcgtactt ggctaaatag
      241 gctgcgtctg cttcggcttc gctttcaaac gctttgaatt gcgcacccac cacgcccttc
      301 acctgttttt cacattccgc ccaagacgtg aagatgccgg tcttgcgtcc tttccatacg
      361 acgtaatatt tctgacgcgc catgcgtcaa ttttacatca attcagtttt gaatatttgt
      421 cttgacaaac gacatcaatc acgtaaaata cgcgctcaat gaaaagtact tcgcaatttt
      481 gctcctgtct gtggcggggt tcgtaaccac cgcctgagac aatatccgcc caagtaagca
      541 gtcaactctc aaggcagtgg aacggctccc gcgtgaaatg atgtccgcgc aggatggttc
      601 gctgtccttt ttgttcggtt ggctgctttt tattttgcat atacttcttg cttagaaaag
      661 gaaactttat catgtcgtac gctcaccccg aataccttgt agaaacagac tgggtcgctg
      721 aacatctaaa tgaccccaac attcgtatta tcgaatcgga cgaagatccc ttgctgtatg
      781 ccataggtca cattcccggc gccgcccaag tagactggtt cagcacgctt caacacccac
      841 tgcgccgcga ctttctcacc aaagaacaat ttgaacaggt tgcatccaag cttggcatca
      901 ccaatgatac aactgtcatc ttttatggcg ataagtcgaa ttggtttgcc tgctatgcgc
      961 tctggctctt ccaatactat ggtcaccaga acgtaaagat cgtaaacggc ggacgtgcga
     1021 aatgggaaaa agaaggtcgc ccactcgtga aggaagttcc cacattcact gcaactacct
     1081 acaaagccaa agaagccgat aagtccattc gcgccttccg cgacgatgtc ttcaagcagg
     1141 ttgattcaaa gaaacccctg gtagacgtcc gctcgccgaa ggaatatacg ggcgaattat
     1201 tgcacatgcc caactaccca caagaaggcg ccacccgcgg cggacacatc cccggagcgg
     1261 taagcattcc gtgggcacag gctgtcaacg aagccgattc cacctttaag accgccgaag
     1321 aattgaagtc cctttacgaa ggcaaaaaca tcaaatccga tggtgaaatc atcgcctatt
     1381 gtcgtattgg ggaacgctct tcgttgactt ggttcgtatt aaagtatttg ctgggttatc
     1441 ccacggtcaa gaactacgac ggttcctgga ctgaatgggg caacctcgta gatgcaccaa
     1501 ttgaaaaata atagtattgt agaggcgagg tttatatcac agagtggtat aacctcgccc
     1561 ttacccggaa cattatgacc ctacccaata aacttcaaga aattgtagat gactttgcca
     1621 gcatgagccg cgaagaaaag ctggagacgc tgatcgcata cgccgaatcc atgccagagt
     1681 tgccagagcg cttccatgca gaacgcgaaa aaatggaagc ggtgccagaa tgcatgacgc
     1741 ccgttttcat tgccgccgag aaagacagca acaacggcat cgtcttccac ttggatattc
     1801 cgaaacaatc tcccaccgtg cgcggactgg catcgattct gaccaatggc ttaaatggct
     1861 gctcgcttgc agagattatt tctgtgcctg ccgagtttta cctgcctatg aatctacaag
     1921 aagccgtatc ccaacagcgc attaatggtt tcatgggtgt actggcacac atgaaacaag
     1981 ttgctgtgcg ggttgggtca gggtaaattt tacaagtaac aaaaaacagt ccctttacgg
     2041 gactgttttt attttctagc cttcaatcca accttgttct tccactgcca taaaaatatc
     2101 ggtggcagag atcaatccga tcaaggtgcc atgttcatct gcaattggca agtggcgaat
     2161 ttttctttct tgcatcagtc gagagcattc tgccagcgtc cattcggcac gcccggtaac
     2221 aatgggacca gacataatct cgcgcacagt ggtctctgct gggttacgcc cggcaaccac
     2281 gatcttatcg cgcacatctg ttgttgttac aatgccatat gaagggttct tctcgctaac
     2341 atccacaact acgctatgaa tattgcgacg acgcatgagc gtcatggcat gtgaaacgct
     2401 cgaatcaggg tctacaacaa caaccgggct ggacatccaa tcacgaacta agttattcat
     2461 aattctctct cctttataga tattttacag tatgggtgta actatcaggt tactcacagc
     2521 gtaaccaaag ggtcgagcaa caaaatcaaa cgtcggcggg gaaagaagcg tttactgact
     2581 tagaaccgtc cacgttgagc cgctatccgt tgtcttatac aaagtatagt ttccagaagc
     2641 atcagaagca acgatccaac cagtttgagc attcgcaaaa ctcatatcca tgatggcgtc
     2701 gccgaacttc acatcgggaa taatttcttg gacagtaaca cccgcatctt tcgtgacgta
     2761 gaactggtca ttgacataaa agaccatttc attcgctgac ggcgtttcca ataatccata
     2821 accttcaaac gaaacttcag attgcgtcca cgtgttaccg ccatcttggg tgacgtatac
     2881 aatggtcttg ggtgtttctg tggcaacccg caacgcaagc agacctgtct tgggcgagag
     2941 aaagaccatt ccgatcacgc tcagttcact gcctgctgct tcttgcggaa ggataaggtt
     3001 gatattgacc caggttttgc cattatcgtc actgcggaaa agataggttt caccgggggc
     3061 atagaccacg ccaccgatcc aagctgtttt tgcattgagc ggcaagatga gattcttaat
     3121 accacccaac ggaagcgtat cacttgcatc agcaagattg gggtcattgg tgtaagtgcg
     3181 attccaagtt ttgccagcat cactggtctg gaaaacagaa accgccatag aaccagcacc
     3241 aacaccaagg tcagatagca tccatccgtt gtttgcatca acaaattgaa tataaccgcc
     3301 gctgaatggc gtagaaaagc tttcccatgt taagccgcca ttggtggtgt gatagagcgt
     3361 accaccattt ggatacttat tcatatcggg gaattgcacc caagccgtat tctcatccag
     3421 aaaatcggtg ttgacgagat agccggcatc cttcaagttt ttcggggtga cgtcatacca
     3481 ggtcacaccg ccatcgttgg tgcggacaac tttttcttcc gtgagtgccc atccgttcac
     3541 ttcatccaac atttggatgt taagaatagc aggagactca atgatgggcg cttcggcaac
     3601 agcaggcaaa ggtgtattga gcactgcttg ggataaagct tgaggttctt ctgtgcctgt
     3661 cgccgcaggt tctgaagcct ccggagccgc cgttgttgct acggcaggag cacaagcact
     3721 caccagcgcc gtaagaataa ataagaaaga gagaaatcgt ttcatggttc tcctttggct
     3781 atcaataaat tatccaagat attcgatcac ggtcgcttcg ccctgcccca cacccacgca
     3841 aagagtcgcc acagcgtatt tgacattacc gcgatttttc atctcatgca caagcgtggt
     3901 catgaggcgc gcacctgaac acccaagcgg atgcccaatg gcaatcgcgc cgccgttgac
     3961 gttgaccagt tcggggtcta tgcccaagtc ttcgatcacc gccagagatt gaacagcaaa
     4021 ggcttcgttc aattccatca gtccaatgtc ttgcattttt agaccggcgc gctctactgc
     4081 tttcttcgtg gcaggcacag gaccgatacc catcacacgc ggaggcacac ctgccgaagc
     4141 agaagtcaca atgcgggcaa gcggcttgag gttgagagcc ttcgcctttt cttcactcat
     4201 gatcaacagc gccgatgcgc catcatttaa tccagacgaa tttcctgcgg tcacagttcc
     4261 cccgtcgcga aatgcggctt tgagcttacc aaggctttcc atcgtcgtgt cgcggcgcgg
     4321 acgttcatct gtgtctacaa ctttcgggtc gccttttttc tggggaatag taacaggcac
     4381 aatttcttgt ttgaagcgcc ctgagtcaat cgcggcaatg gcacgttgat ggcttcgcat
     4441 cgcaaaggca tcctgttttt cgcgtgtgat gtgtggacgt tccgcagcaa tattctctgc
     4501 ggtctcaccc atcgagtcag ttccgtgcat cgccttcatt ttgggatttg ggtaacgcca
     4561 gcctagagcc gtgtcatatg cagtgagatt gccgaaggaa aaccccgcct cggctttggg
     4621 aagtgaatac ggtgcacggc tcatagactc cacgccacct gcaatgtaca catccccttc
     4681 accagctttg atggctcgcg acgccatgtt gatggcgttc aaacctgaag cacacaagcg
     4741 gttgacggtc acaccaccca cttccacggg cagaccagca agtaatgccg ccatacgtgc
     4801 tacattacga ttatcttcgc cagcctggtt cgcacacccc ataaagacct cttcgatctg
     4861 tgcagggtca atttgatttc gttcaagaat ggatttgatc acatgtgccg ccatatcgtc
     4921 tgggcgcaca gatgaaagaa tgccgcccaa cgcgccgatc ggcgtgcgga tggcatcaac
     4981 aattacaggt gtggacatgc gtccctccag agtttgaatt cataaattga ttctaacacc
     5041 agagagagca gttcttgtta atctgtagaa cggtttgtaa taatcaacgc ttgcagatgt
     5101 ttattgaaat tcttaacatc tgttcccaag ctgttgaaaa aatcatggtc aattttctca
     5161 accacgccaa tagtttgagt agccaactta cgtccctgtc cggtaatgga caatgctttt
     5221 gctcgggtat cagtcgggtg cggtttacga gtaacgaaac ctttgtcttc cagcgttcgt
     5281 agcacgttag acaccatcat cacatccatt ttggcatgat tggcaagatc cacctgtgta
     5341 agaggtttat cttcacctgc caaccacatc aaagatgcca gtaaaacaaa ttgcgcgtgc
     5401 gtgaggtcaa aaggcttcag ccctgcattg atctgtctct gccacatgct ggaaacctgc
     5461 caaagcgaaa accctgggc
//
LOCUS       DAOQFT010000089         5464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7575_length_5464_cov_5.863930, whole genome shotgun sequence.
ACCESSION   DAOQFT010000089 DAOQFT010000000
VERSION     DAOQFT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7575_length_5464_cov_5.863930"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..734
                     /locus_tag="PLR93_03500"
     CDS             33..734
                     /locus_tag="PLR93_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26055.1"
                     /translation="MKHRIFQKRFTWACVAFLLIGCSPATTIVPESTATPYSTFTPID
                     TPTNTLTPTPYPSATIVKIPTWDPNQPTFTPFAQFSNIFPTGTSSKPGPGFASVTYSP
                     TKIYWGGCTPNSVTIIAKVDDVEEVTSVLIFVRVKDLTEEDYTPWTNGDVMLNRGQGE
                     FTTVLYGSKIKGHDHYLRSWVYFQLVATNIEGEEIGRTKIYDKAFDMYPCPCLTPLTG
                     CPIATPKPPSPTPKK"
     gene            738..1421
                     /locus_tag="PLR93_03505"
     CDS             738..1421
                     /locus_tag="PLR93_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26056.1"
                     /translation="MRKIFLLTLLLIFSLACSLGTFAPQPTATPAPTQTDTATPLPTP
                     TPVTPTLTFTSTPTLISNTAIPTIETTSTELSTPTATQALATPYTVMPPVNIKGFISI
                     TISLTEVFKAKGCEPSVVRVTAQVANPNDVSFVLLFSRFKSLKAERTGKWTTVQMIPL
                     GAGTYIFDLSSALMKDDAYFENAWIEYQVVSTNQAGTELGRTDIFKERLKMVECIFTA
                     TPTSATVKP"
     gene            1422..2159
                     /locus_tag="PLR93_03510"
     CDS             1422..2159
                     /locus_tag="PLR93_03510"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNH26057.1"
                     /translation="MNFPADFVSFLTSATRVVVLTGAGVSQESGLRTFRDAQTGLWAR
                     YKPEDLASPEAFSRDPKLVWDWYAWRREAIKGVRPNPGHYALVEMEKRIPQFTLITQN
                     VDGLHRFAGNQNVLELHGNIQKVRCSECRTFTEEWGDDMEGVPTCSVCGGLLRPDVVW
                     FGETLPRAELEAAVTASRAAQVYFSIGTSGVVQPAASLAHAARNNGSVVIEINAEPTP
                     LTPKVDFAFHGKSGEILPALIKTVWGN"
     gene            complement(2156..2560)
                     /locus_tag="PLR93_03515"
     CDS             complement(2156..2560)
                     /locus_tag="PLR93_03515"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH26058.1"
                     /translation="MNTQNILELRVAITTKDYERLVKFYCLGLGIEPAAVWNNDGGKA
                     LMFNMGNATMEIFDERQAEVVDDLEVGERVSGQIRFALQVPDLNAAMERMLANGAILV
                     HEPVLTPWGDYNVRLQDPDGMQITLFQAKENG"
     gene            complement(2579..>5464)
                     /locus_tag="PLR93_03520"
     CDS             complement(2579..>5464)
                     /locus_tag="PLR93_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26059.1"
                     /translation="IRRRYDFDFTPAATTTSSAPQPQAIAALATPQATPPVTTSTPPE
                     PRPSLLQTLTSEASIKIYLYLGAFFVIAAAAILGAVVPELRLPILIIGTFIFGGLSVV
                     IKKRLPQPSFALFIVFSFLLPITANSLEESLRQAFNLTADFTDAYWATIFIVMAIIWI
                     GSTWLYESRLFSLTAFGSLILASVRIGDLFEADAVYYTLLMGLTALVGLGGTWLLKKW
                     RDEKFAQPLFLASQLLQALNLATSLGIFGVNTFQSSGNNILNLITLATWITGTLFYIV
                     SEWLYPFFAFPWLAAATFIPMPWLIASAFDLESLGSSIVLFVWGAILAVASEPIIRSE
                     KLKKYGLPILLAAMPSLALGIFTGFIYETWLGLTLALGTAILFTSLHILQNRWWLWTL
                     ALLNFVAAYFAFFQLEAIQKIDIFFGYQLLGITILYLLPDLFLKKDWMLNLQWRLPLR
                     IFGAAFLFITSIVLFTQDKSGHIAINYFILALFCTAYALAYRNAYLAYLPAAYLALTT
                     VYALDAFNVDAWLPALTGLSVIYYLAGVFLRKQEKWGNMFRNSGMALGTILALVALSA
                     TKETGGWYALIIGLLFISETHLRHNGWFEIGVPIHFSIGVFLILHDFKVEQIPYQLLA
                     HSLVWLLSDLLTHLTFKDPRPLRVIVRLIGGTLVLVNFAFLFAETDSWIPVIGFGIYT
                     LLFLTISLLYRQPTLLYTFTLTLPLFVAFLFRAFEITQWIHPVIAVAVAYYVLGTTLR
                     VKNRATGWDQSLLYSGLGVGVIVSIGAPILGGLDASIPVAIAATLWAFEAFIKRNAWL
                     AFPANALYLLAYFIILFELNVNEPQFFSVGTALFGLLQHYLLTRAESKSGTFIMGMFS
                     QFVLLGTTYIEMVNKNELGYFFLLFVQSLVILVYGLVIRSRSLTLFPIGFVALGVITV
                     VYSTLKDLGTIFVIGCTGIVLLMLGVGAVLLRERIAKLGEKLSDWKA"
BASE COUNT         1602 a         1246 c         1264 g         1352 t
ORIGIN      
        1 ttacacatta gatttcatag tattatagcg ggatgaaaca tcgtattttt caaaagagat
       61 tcacttgggc ttgtgtggca tttttgttaa ttggctgttc tccagcaacg actatcgtcc
      121 cagaatctac agctacgcct tatagcactt tcactccaat tgatacccct acaaatacgc
      181 ttacccctac tccttaccct tcagcgacca ttgtaaaaat tccaacttgg gatccaaatc
      241 agccaacctt tacaccgttc gctcagtttt ctaatatctt cccaacagga acatcttcga
      301 aaccaggtcc tggcttcgct tccgttactt attcaccaac aaagatttac tggggaggtt
      361 gcactccaaa ttcggtaaca attatagcga aagtagatga cgtagaagaa gtgaccagcg
      421 tgcttatttt cgtccgcgtg aaagatttaa cggaagaaga ttacacccca tggaccaatg
      481 gcgatgtgat gttgaaccgc gggcaaggtg aatttacaac cgtgctttat ggtagcaaaa
      541 tcaaaggtca cgatcactac ttgcgctcat gggtatactt tcaattagtg gccacaaata
      601 ttgaaggtga agagattggg cgtaccaaga tttacgataa agcatttgat atgtatccat
      661 gcccgtgcct tacgccactt actggctgcc caatcgccac accaaaacca ccatctccaa
      721 ctcctaaaaa ataaaccatg agaaaaatat ttttacttac gcttcttctt attttctccc
      781 ttgcttgttc attagggacg ttcgctccgc aaccaactgc aaccccagcg ccaacgcaga
      841 cggacaccgc aacgcctctt cctacgccaa cgcctgtaac gccaactctg acgtttacct
      901 caactccaac actcatcagc aatacagcta tcccaacaat tgaaaccact tcaaccgagc
      961 tttcaactcc aactgccaca caggcattgg cgacacctta tacagtgatg cctccagtaa
     1021 atattaaagg ctttatatct attacgattt cactgaccga ggtttttaag gcaaaaggat
     1081 gtgagccttc tgttgttcgc gttactgctc aagtagcaaa cccaaacgat gtctcatttg
     1141 ttctcttgtt ctcccgtttt aagagtctga aagcagagcg caccggtaaa tggacaactg
     1201 ttcaaatgat cccattgggt gcaggtacat atatattcga tctttcttct gcgcttatga
     1261 aagatgatgc ttatttcgaa aacgcatgga tcgaatatca agtcgtctct acgaatcaag
     1321 ccggcacaga attagggcgc actgatattt tcaaagaacg cctcaagatg gtggaatgta
     1381 tctttactgc cacccccaca tctgccactg taaaaccgta gatgaatttc cctgcagatt
     1441 ttgtcagctt tttgacttct gccacacgag tcgtcgtact cactggagcc ggagtctccc
     1501 aagaaagcgg actccgcacc tttcgcgacg ctcaaaccgg actttgggct cgatacaaac
     1561 cagaagattt agcctccccc gaagcttttt cccgcgaccc gaagctagtt tgggattggt
     1621 acgcttggcg tcgtgaggca attaagggcg tccgcccaaa ccccggacat tatgcgctag
     1681 tagaaatgga aaagcggatt ccccaattca ccctcatcac ccaaaatgtg gatgggttgc
     1741 atcgctttgc tgggaaccaa aatgtattgg agttacacgg taatattcaa aaggtgcgtt
     1801 gttccgaatg ccgaacgttt acagaagagt ggggggatga tatggaaggc gttccaacct
     1861 gctcagtctg tggcgggctt cttcgaccag atgttgtttg gtttggagag accctgccaa
     1921 gagcagaact tgaagcggca gtcactgctt cacgcgctgc acaagtttat ttttcgatcg
     1981 gcacatcagg tgtcgttcag cctgctgcgt ctttagcaca tgcagcccgc aataatggtt
     2041 ctgtagtcat agagatcaac gccgagccaa ccccgcttac acccaaagtt gattttgctt
     2101 ttcatggtaa gtctggcgaa attttgcctg cgcttattaa gacggtttgg ggcaattagc
     2161 cgttctcttt tgcctgaaat agcgtgattt gcataccatc cgggtcttgc aaccggacat
     2221 tatagtcacc ccatggtgtc aacacaggtt catggacgag gatcgcacca ttggcgagca
     2281 tccgttccat ggcggcgttt aagtctggta cttgcagtgc aaatcgaatt tgcccgctca
     2341 ctcgttcacc aacttcgagg tcatcaacca cttcggcttg acgctcatca aaaatttcca
     2401 tggtagcgtt tcccatatta aacatgagag ctttgccgcc gtcgttgttc catacagcgg
     2461 caggttcaat tcccagtccg aggcaataga acttcacgag tctttcataa tcttttgtag
     2521 taatagcaac tcgaagttct aaaatatttt gtgtattcat ttaatagtcc tcttgaaatc
     2581 aagctttcca gtcgctcaac ttttcgccta gtttcgcaat tcgttcgcgt aacagaacgg
     2641 cgccaacccc gagcataagc aggacaatgc ctgtacaacc aattacaaaa atcgttccaa
     2701 ggtcttttag tgtgctatat acaactgtaa tcacacctaa agctacaaac ccaattggaa
     2761 ataaggttag actgcgtgaa cgaatgacaa gaccataaac aagaattaca agcgactgaa
     2821 caaatagcag gaagaaatag ccaagttcat ttttgttcac catttctata taggttgttc
     2881 cgagtagaac gaattgagag aacatgccca taatgaatgt gccagatttg ctctctgcgc
     2941 gtgttagtaa ataatgttgg agtaaaccaa atagagcggt tccgactgag aagaactgcg
     3001 gctcattcac attcaactca aagaggatga tgaaatatgc cagcagatat aaagcatttg
     3061 ctgggaatgc cagccaggca ttgcgtttga tgaaggcttc gaatgcccaa agtgtagctg
     3121 caatggcaac tggaatcgac gcatccaacc cgccgagaat gggtgctcct atcgaaacaa
     3181 taacgcctac gccgagaccg ctatatagca gggactgatc ccagcctgta gcacggtttt
     3241 ttactctaag ggttgttcca agcacataat aggctactgc caccgcaatg acggggtgaa
     3301 tccattgggt gatttcgaaa gctcgaaata ggaaagcgac aaaaagtggc agtgtcaaag
     3361 tgaaggtgta gagtaaagtt ggttggcgat agagcaggct tatggtcagg aagagaagcg
     3421 tgtaaatccc aaatccgatg acgggaatcc aagagtcagt ttcagcgaag aggaaagcaa
     3481 aattgactag cacgagagtc ccgccaatca agcgaacgat aactctcaag ggtctggggt
     3541 ctttgaatgt taagtgagtg agtaaatctg aaaggagcca aacaagagaa tgtgccaaca
     3601 actgataggg gatttgttca accttgaaat catgcaggat gagaaacacg ccaatgctga
     3661 agtgaattgg aacgccaatt tcaaaccaac cattgtgccg caaatgggtt tcagaaataa
     3721 agagtagccc aataatcagt gcataccaac cgccagtttc ttttgtggcg cttaacgcta
     3781 caagcgccaa gatggttcca agagccatgc cgctattgcg gaacatgttg ccccattttt
     3841 cttgtttgcg aaggaagacg ccagctagat aataaatgac ggacagaccc gtcaatgcag
     3901 gcaaccatgc atcgacgttg aaagcatcta atgcgtatac ggtggttaac gctaaatacg
     3961 cggcgggtag atacgcaaga taggcattgc gataagcaag cgcatatgct gtgcagaaca
     4021 acgcaagaat aaagtaattg atagcaatat gcccgctttt atcttgggta aatagcacaa
     4081 tgctggtgat gaagagaaat gctgcaccaa aaatccgtaa cggcaaacgc cattgcaagt
     4141 tgagcatcca atcttttttt aggaataaat ctgggaggag ataaagaatg gtaatgccta
     4201 ataactgata gccaaagaag atatctatct tttgaatcgc ctcaagttgg aagaacgcaa
     4261 aatatgcagc cacaaaattg agcaacgcta atgtccagag ccaccagcga ttttgaagga
     4321 tgtggagact ggtaaagaga atggctgttc cgagtgccaa agttaaacca agccaggttt
     4381 catagatgaa gccagtgaat attcccaatg ccaagcttgg catggctgcc aatagaatag
     4441 gcagaccgta ctttttgagc ttctccgaac gaatgatcgg ctcgcttgca actgctaaaa
     4501 tggcacccca aacaaacagc acaatggatg agccgagact ttcaaggtca aatgcactgg
     4561 cgattagcca cggcatgggg ataaaggttg ctgcagcaag ccatggaaaa gcgaagaaag
     4621 gataaagcca ttcagagact atgtaaaaaa gggtacctgt tatccatgtt gcaagtgtga
     4681 ttagatttag gatattgttg ccggatgatt ggaacgtgtt tactccgaaa atgccaagag
     4741 acgtggctag atttagagct tggagaagtt gggatgcaag gaatagaggc tgggcgaatt
     4801 tttcatccct ccattttttg agaagccagg tgccacctag acctacgaga gccgttaacc
     4861 ccataagaag ggtgtagtac acagcatccg cttcgaatag gtcaccgatg cggacacttg
     4921 ccagaatcag agacccaaat gctgtgagtg agaacaagcg agattcgtac aaccaagtgc
     4981 taccaatcca aataatagcc atcacaatga aaattgtcgc ccaataagcg tcggtgaaat
     5041 ccgctgttaa gttgaaagct tgacgtaggc tctcttcaag gctattagcg gtgatgggta
     5101 ataggaagga gaaaacaatg aataaagcaa agcttggttg cggaagtctc tttttaatga
     5161 cgacggagag accgccgaaa ataaaggtgc caattatcag aattggcaaa cgtaattctg
     5221 ggaccactgc gccaaggatg gcagcggcgg caatgacaaa aaacgctcca agatatagat
     5281 aaatcttgat gctggcttca ctagtaagag tctgtaacag agatgggcga ggctcaggtg
     5341 gcgtggatgt ggttactggt ggagtcgctt gcggggtggc aagggctgct atcgcctgag
     5401 gttgaggcgc agaggatgta gttgtcgctg caggagtaaa gtcaaagtcg tatcggcggc
     5461 ggat
//
LOCUS       DAOQFT010000090         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7585_length_5459_cov_6.512768, whole genome shotgun sequence.
ACCESSION   DAOQFT010000090 DAOQFT010000000
VERSION     DAOQFT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7585_length_5459_cov_6.512768"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLR93_03525"
     CDS             complement(<1..340)
                     /locus_tag="PLR93_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH26060.1"
                     /translation="MTQHRITDDLEALLDVLPANIRHAVEKANNSDKLLEIVIDLGRI
                     PTARFVDGEIPLLNTEITRAEIDHITERIGTFDADNRAGMERTLHRISAIKNRRSAIV
                     GLTCRVGRAVY"
     gene            complement(680..1570)
                     /locus_tag="PLR93_03530"
     CDS             complement(680..1570)
                     /locus_tag="PLR93_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26061.1"
                     /translation="MKEPRVYKTSPILLVFIGLLFLFFIGILLFAIGPNSVGFMIPVG
                     VFSLFLFGAAFISMASKTIVSDDEIAVQGLLGTKSINWANVGRVSGWGYAMKLHSRDD
                     DVTLSISPRLPGYQEVIEFVGSKRPDLFSVQEYGSMKRGITQYLAVLLGLLMMGGVAV
                     AYIVAIMSNEQNITSSSFMPLLIIAVFALIMIVTTLSGPHSINLEGNSVNLKYLFSEK
                     SFQSNEIANVQLSFTQSRNGRHYFIALNLLSRKTIRISGLGMSLPIAYLVLKNWHLGR
                     STGSGAQTNIAPNWSDNTWR"
     gene            1681..2994
                     /locus_tag="PLR93_03535"
     CDS             1681..2994
                     /locus_tag="PLR93_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNH26062.1"
                     /translation="MSIPEEPNLPSFSPQDETPAPPPPIRRRRATRRSHIPTDAEGQA
                     ELIASLARRAYPSIELFVFSLVCGAILGLGYLLDSQALLLLGVLVAPLMTPWVGFLLS
                     ILSGSLRFMFETLMALLISIIFVFLGGLLTGLAALPFMPRTFDNVFLHSRLWIPELIV
                     FALGAIILVISFARSEDKPFLPSVLIAYSFYLPISAAGFGIGSGLPNVWLQGLLVFLV
                     HFAMVSIIGLITLFVLRLRPSAGGVVLSVVTLVIFGGVIAFVAGSGSPSRNDAVPAST
                     PTNVITSAVPSLTPSLVAVPNTNTTPTVKSATSTPTVSSFGSITPSPVPLTLEITLPP
                     TDTPTITLTIQPTPTYGKVQANEGGGANLRDAPGGTYVMTLLNGTIVETFSEFQVLNN
                     ITWVKVYAQVNGQRIEGWLLESVISYATPAPNFQASPTPQGTPTP"
     gene            3067..4515
                     /locus_tag="PLR93_03540"
     CDS             3067..4515
                     /locus_tag="PLR93_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNH26063.1"
                     /translation="MTIHFGTDGWRAVISDTFTFSNLRMVSQAIADAVASEHWDKSEN
                     VDEKKIVIGYDTRFLSDRFAGEVGRVLAANGFSVLLAQSDSPTPAISYAVKNNNAIAG
                     VMITASHNAPRYNGVKLKGAFGGSALPEQCRRVEVYINDNEQQARGPNLMDFKKARDA
                     GLIQRFNPLPAYFDHLRKLINTDIIADNPQRFVVDAMYGSGRGVIKSFLQGTGCEIHE
                     IRGEMNPGFGGIHPEPIAKNLGALSSAVSSGLGNFGVVTDGDADRIGAMDERGNFVDP
                     HKIMALSLKYLVEKRGMTGAVVRTVSTTRMLDRLAKRYGVKIYETPVGFNHIADIMMN
                     EDVLIGGEESGGISFKGHIPEGDGPIMGLLIVEMLAAYGKSLGELVDDLLADVGPAFY
                     ERVDLRLSRPVAKAEMTEFLTKNAPAEIGGATVAEISQRDGVKYIMSDDSWLLVRPSG
                     TEPVLRVYAEGRTQEMVKALLGYGKTVAESVV"
     gene            complement(4556..>5459)
                     /locus_tag="PLR93_03545"
     CDS             complement(4556..>5459)
                     /locus_tag="PLR93_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26064.1"
                     /translation="MSISQALEVAIGLIFVYYVLGSIVSLVTQWINESFETRGKSVER
                     HLMKIVGDKHVDEFVKLPQLQALRPIRYKNMFSFLTSSTEPKKLEKIPAATLVDSYFD
                     FVGLTSQEFKADELKDFINAFPESEGKQAMLKWVSQGVTNIEDLRKRTTTYFSGVMDQ
                     AASTFKSNARSFVIMLSIFLTLLLGTDSIQLAQTLWANASVRAIAVAQAQAVVQQEGS
                     NANVDDLLQQLLDLQIVKIGWWQTDLPQTTDIGTWGGFIALKFLGLGLTAVAVSQGSS
                     FWYDLLKKIVSPSKGGGGGDEAKG"
BASE COUNT         1422 a         1399 c         1240 g         1398 t
ORIGIN      
        1 catatacggc acgacccaca cggcaagtta aaccgacaat ggcgctgcga cgattcttaa
       61 tcgcagagat gcgatgaagc gtgcgctcca tgcctgcgcg gttatcggca tcgaaggtac
      121 cgatgcgctc ggtgatatgg tcgatctcag cgcgggtgat ctcagtattg agcagcggaa
      181 tctcaccatc cacaaaacgc gcggtgggaa tgcgcccaag gtcaatgacg atctcaagca
      241 gtttgtcgct gttatttgcc ttctcaaccg catggcggat attcgcaggc agaacatcca
      301 acagagcttc gaggtcgtct gtgattcgat gttgagtcat aatgttaagt ttccagaaac
      361 gccaagggtc aagtggcgtg aaaagtcaaa gggaaaagaa tatatcggct cgcgcaaaac
      421 aaaagacgcg gcttgtctta agggtgaaag agggggaagt tgaaaaggat catgatggtg
      481 tacctgcctt cctggggcgt cacgcgcaga gtttcgcgtg tcaggcatcc tgtgccgtta
      541 tgtagtggca aaggctatta tagcaggtac gcgattaatg gtttgttagc aatctatatt
      601 aatttcatca atttgtctga cgaattaaat ccgttcagca tcgcaaattg tctagtctgc
      661 gatgctgaac agttctaccc taacgccacg tattgtcaga ccagttcggg gcaatattcg
      721 tttgagcacc tgaacccgta gaacgcccaa gatgccaatt cttcagcacc agatacgcaa
      781 ttggcaaact cataccaagc ccagaaatgc gaatggtctt ccggctcaat aaattcaatg
      841 caatgaaata atgcctgcca tttcggcttt gtgtgaaaga aagctgcaca tttgcaatct
      901 cattagactg aaaacttttt tcgctaaaca ggtacttcaa attcacagag tttccttcta
      961 gattgataga atgcggtcca gagagggtgg tgacaatcat aatcaatgca aacacagcaa
     1021 taatcaaaag cggcataaat gaagaggagg ttatattctg ctcgttactc atgatcgcaa
     1081 ctatgtaggc gaccgcaacc ccacccatca taagaagccc caataagacc gccagatatt
     1141 gggtgatacc gcgtttcata ctgccatatt cttgcacact aaaaaggtct ggtcgcttcg
     1201 aaccgacgaa ctcgatcacc tcttgatacc ccggcaaacg cgggctaatg gataaggtca
     1261 cgtcatcgtc cctactatga agtttcatgg catagcccca gcccgaaacc ctgccaacat
     1321 tcgcccaatt aatactcttt gtgcccaaca atccctgcac ggcgatctca tcatccgaga
     1381 caatcgtctt ggatgccatt gagataaatg ccgcaccaaa taaaaacaaa gagaaaacac
     1441 ctactggaat cataaaccca acggaatttg gtccgattgc gaaaagtagt attccaataa
     1501 agaataaaaa caaaagaccg atgaacacca aaaggattgg tgaggtctta tacacacgtg
     1561 gttctttcat aataaggctc ctatttcagt tctgaatgac aaggtcccaa ctatacgatg
     1621 aagatattgt aatactccat cacctctctt tcctacccgt tattcctgta taattgccat
     1681 atgtccatcc cagaagaacc caacctgccg tccttctcgc cgcaggatga aaccccggct
     1741 ccacccccac ccatccgccg cagacgcgcc acgcgccgtt ctcacatccc taccgatgcg
     1801 gaaggtcagg cagaactcat tgcgtcgctt gcacgccgcg cctacccctc cattgaactc
     1861 ttcgtgttct ctttggtatg tggcgcaatc cttgggcttg gctacttact cgactctcaa
     1921 gctctgctct tacttggcgt tcttgttgct ccactcatga cgccttgggt gggctttctc
     1981 ttgtccattc tttctgggtc tttgcgtttc atgttcgaaa cgctcatggc attgctcatc
     2041 agcatcatct ttgtttttct tgggggcttg cttactgggt tggcagcact gcccttcatg
     2101 ccccgtacct tcgataatgt ctttttacac tcgcgcctgt ggattcctga attaatcgtc
     2161 ttcgccctcg gcgcaatcat ccttgtcata tcattcgcac gctctgaaga taaacctttc
     2221 ttaccaagtg tgctaattgc ttattcgttt tacttaccga ttagcgccgc agggtttggc
     2281 attggctcag gtctgccaaa tgtgtggttg caaggcttgc ttgtgtttct tgtccacttt
     2341 gccatggtca gcatcattgg tcttatcacg ctctttgttt tgaggctgcg tccatctgca
     2401 ggtggagttg ttttgagcgt ggtaacgctg gtcatcttcg gcggagtcat cgcctttgtt
     2461 gcaggatcag gctccccgtc aagaaatgat gcggttcctg cctccacacc aaccaatgtg
     2521 attactagcg cggttccttc tctaacacca agtttggttg ctgtccccaa tacaaacacc
     2581 acaccgacag ttaaatctgc cacgagcaca cccactgtct catcttttgg cagcattacg
     2641 cccagccctg tgccactcac cctagaaatc actttgccgc ccacagacac gcccacgatc
     2701 acacttacca ttcaaccaac ccctacatac ggaaaagttc aggcgaatga aggcggtggt
     2761 gcaaacttac gtgacgcccc aggcggcacc tatgtaatga ccttgctcaa tgggacgatt
     2821 gtagaaacat tctctgaatt ccaagtgttg aataacatta cttgggtgaa agtatatgcc
     2881 caagtaaacg gacagcgcat tgaaggttgg ctgttagagt ctgtcatttc ttatgcgacc
     2941 ccagcaccga acttccaggc atcgcccacg ccgcaaggta cacctacccc ataaattcat
     3001 gttcttaagg tgacaggcta ttacgacgtc aacacctttt catatcactt tacttcagga
     3061 gactcaatga ccattcattt tggcaccgac ggctggcgcg ccgtcatctc agatacattt
     3121 actttcagta atttgcgtat ggtttcgcaa gccatcgcag atgcagttgc ttcagaacac
     3181 tgggataaat ctgaaaacgt ggatgagaaa aagatcgtaa ttggttacga cactcgtttt
     3241 ctttcagatc gttttgctgg cgaagtaggg cgtgtactcg ccgcaaatgg atttagcgtt
     3301 ttgctcgcac agtcagattc ccccacgcca gcaatttcat atgctgtcaa aaacaacaat
     3361 gcgattgcgg gggtgatgat cacagcatca cacaatgcac cacgctacaa cggcgtgaaa
     3421 ttaaagggcg cttttggtgg ttcggcactc cccgaacaat gccgccgcgt ggaagtgtac
     3481 atcaatgaca atgaacaaca agcccgcgga cccaacttaa tggatttcaa aaaggcgcgt
     3541 gacgccgggc taattcaacg cttcaacccg ctccctgctt atttcgacca cctccgcaaa
     3601 ctgatcaaca ctgacatcat tgcagacaat ccgcaacgct ttgtagtgga tgccatgtac
     3661 ggatctggtc gcggggtgat caaatctttc ttgcaaggta cgggatgtga aatccacgaa
     3721 attcgcgggg aaatgaatcc tggtttcggt ggaattcacc cagaacccat tgccaagaat
     3781 cttggtgcgc tctctagcgc ggtcagctcg gggttgggca actttggcgt ggttacagat
     3841 ggtgatgcag accgcatcgg tgccatggat gaacgcggca actttgttga cccgcataaa
     3901 atcatggcgc tttcgctcaa gtatttggtc gagaaacgcg gcatgaccgg tgccgtggtc
     3961 cgcactgttt cgaccacacg catgttagac cgcctcgcca agcgttatgg ggtaaaaatc
     4021 tacgaaacgc ccgtcggttt caatcacatt gcagatatta tgatgaatga agatgttctc
     4081 atcggcggcg aggaatctgg cggcatttct ttcaagggac acatccccga aggggatggt
     4141 cctatcatgg gcttgcttat tgttgaaatg cttgccgcat acgggaagtc tcttggtgaa
     4201 ctggtagatg acctgcttgc agatgtggga cccgcctttt atgaacgtgt tgacctgcgt
     4261 ttgagccgcc cggttgccaa agccgaaatg actgagttcc tcaccaagaa tgccccagct
     4321 gaaatcggtg gtgcaacagt agctgagatc agtcaacgtg atggtgtgaa atacatcatg
     4381 tcagatgact cttggctctt ggtccgccca tcaggcacag aacccgttct acgtgtgtat
     4441 gccgaagggc gcacacaaga aatggtcaaa gccctgctcg ggtacggaaa gaccgtcgct
     4501 gagagtgtgg tgtaaacaat aaaaaagagg cacctgaaca ggtgcctctt ttttattatc
     4561 ctttcgcttc gtccccaccc ccaccacctt tggatggaga gacaatcttc ttgagtaaat
     4621 cataccagaa ggacgagcct tgcgaaacag ccacagcggt caaaccaagc ccgagaaatt
     4681 tcaaagcaat aaatccgccc catgtgccaa tatccgtggt ttgaggaagg tctgtttgcc
     4741 accagccgat ctttacgatt tgtagatcta acaattgttg gagaaggtca tccacattgg
     4801 cattgctgcc ttcttgttgc accacagctt gtgcctgtgc caccgcaata gcacggacac
     4861 tcgcatttgc ccacaaggtt tgtgcaagct gaatgctatc tgtgccaagc aacaaggtca
     4921 agaaaataga caacataatg acaaaactgc gagcgtttga cttaaaggtt gaagccgcct
     4981 gatccatcac accagagaag taggtagtcg tacgctttcg caggtcttca atattggtca
     5041 caccctggct tacccacttc aacatcgcct gtttgccttc tgattcaggg aaagcattga
     5101 taaagtcttt taactcatcg gctttaaatt cttgagaagt gagcccaaca aaatcgaaat
     5161 aagaatcgac caaagtcgca gcagggatct tctctagttt tttaggttct gtagaagatg
     5221 taaggaagct aaacatattc ttataacgaa tagggcgtaa tgcctgcaac tgcggaagtt
     5281 ttacaaattc atccacatgt ttatcgccga caatcttcat taggtgtcgt tccactgatt
     5341 taccacgtgt ttcgaacgat tcattgatcc attgtgtgac gagagaaacg atcgaaccta
     5401 acacgtaata aacaaagatc aagccaatgg ctacttcaag cgcttgtgaa attgacata
//
LOCUS       DAOQFT010000091         5446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7624_length_5446_cov_4.148952, whole genome shotgun sequence.
ACCESSION   DAOQFT010000091 DAOQFT010000000
VERSION     DAOQFT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7624_length_5446_cov_4.148952"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLR93_03550"
     CDS             complement(<1..383)
                     /locus_tag="PLR93_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26065.1"
                     /translation="MTDTARATFGQMNTHLSESLDGVEIMKGSAQEEAEVDRFVTNAR
                     KVRDAFVQQGDLEARYIAMLLLGLAFAGGLTHALFLLRAGLINVGAVIGYFGMLQLLG
                     FPTFTSTFAYSQISLGMSSARRRACR"
     gene            complement(380..991)
                     /locus_tag="PLR93_03555"
     CDS             complement(380..991)
                     /locus_tag="PLR93_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNH26066.1"
                     /translation="MTSLDIPAEFTLHRKVKYDHSSPVRWIFSHLSPYWWIMIMLVTG
                     AIGNAVLAVVVPVLTGDAFNAMLAPQPDTSVLLPLAITIGVSQVIRGVLQLGRNFGAE
                     LLAQKMERDIRDELYLSLLGKSMTFHNLQPVGDTMARATNDVREVNYMFSPGVNLVVG
                     SFMFILMPLFFGPRYHPSLVLTPLIFIAIYLWRWYAILRCSHR"
     gene            complement(1295..2236)
                     /locus_tag="PLR93_03560"
     CDS             complement(1295..2236)
                     /locus_tag="PLR93_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNH26067.1"
                     /translation="MTLQLPESFTNLIRSVFEQKGEQLLADLPMLIDESSQRWGITAI
                     EPVPNLSYNFVAYAKRGSEDVILKIGVLNRELLSEMNTLKFFNGEGICQLLESDEEKG
                     MFLLERLKPGKMLSELEDDDERTHIAVEVMQKTCRPVDMESDSLLSDVRQRSAGLQRF
                     IPLTDWLDELKTIRPRFNGGTGPIPARLLERVEDSLPDLFAREGNFLLHGDFHHFNIL
                     SSERGWLVIDPKGVIGPMGYEIGPFMLNPWNMSMDWSRFKVQAARRVDIFHERLGWER
                     EKIIAWALAHSILSACWDLDLDPGYAIEAAEIFAGLK"
     gene            complement(2265..2972)
                     /locus_tag="PLR93_03565"
     CDS             complement(2265..2972)
                     /locus_tag="PLR93_03565"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNH26068.1"
                     /translation="MSTRQFRQLAGLLFIVGTVLINIPYTLLIINFDYPDILRQPTAE
                     ILTRFQAGGNALIYTWLAFAWVGLPMLFGVIMLKQILKDENAPFLELTTTLGVMGFIV
                     QVIGLLRWVFVVPVLARLYTAPGTDSATQAAVSAVFTAVHQYGGVILGEHMGQLLTIL
                     WMSMLSSIIYRSKMFSKWVAVLGWVASAVYLLAQTELLATAIPTFPVVDWAGLVGSLL
                     WLAWMVVVGVYLVKFKE"
     gene            complement(2983..3561)
                     /locus_tag="PLR93_03570"
     CDS             complement(2983..3561)
                     /locus_tag="PLR93_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH26069.1"
                     /translation="MSTREKILDTALTLFNDQGTAAVSTNHIAEAAGISPGNLYYHFR
                     NKEEIIRALFQRLFDTWDNGLPFPHDHQPTLADLKTIIETNYQIIWQYRFAYRELVAL
                     LRQDTELRKNFLAVRQRGFNGFHELIHTFSDAGIIHLPDDPKAIHELQEIVWLISEFW
                     LTNLEVNGRSVNKGEMQNGVKLMLRVLQPYLV"
     gene            3735..4268
                     /locus_tag="PLR93_03575"
     CDS             3735..4268
                     /locus_tag="PLR93_03575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNH26070.1"
                     /translation="MQDFSPIQTKRLSLRPFQADDLDFSFQHFSDPQVAKYLLDEPPV
                     TELSQAQEIVQFYLDSAGKPYNRWVIVSNADGQRIGTCGFHKWNKPHQHAEIGYDLSP
                     KAWGQGYMQEALSAAIQFGFMQMKLHRIEALVYPENSRSLHLLHKLGFKQEGILRDYF
                     YLDGKFYDHVILSLLKT"
     gene            complement(4281..5198)
                     /locus_tag="PLR93_03580"
     CDS             complement(4281..5198)
                     /locus_tag="PLR93_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26071.1"
                     /translation="MLPNIKLLSLSFVFVLMSCQVTPNKPTQLELTKTFTPASVPTVT
                     PVFTNTPVAETVVFEENMSPNGEWTGVVSVTTRDTQKNVNFIVVNNINNQKWTLEDIN
                     FLEPDNPLNGFKYPYVFMWSKDGRYMYYSHLSMGGDGCFGHFKPGGSDLIKFDLLLGI
                     FVVIKEDSATWMALSPDEEKLAFVDMYGGNVSVLDIQKGITASFPLPDLEDAQGYYND
                     TSDIYWSPEGDSLVYAYYIGVCDFIYPASYIIQVFPESNYQKILIENSEESYEPVKWD
                     IEDKILLEDYDNKQWWLDVTTKEISPVNN"
     gene            complement(5230..>5446)
                     /locus_tag="PLR93_03585"
     CDS             complement(5230..>5446)
                     /locus_tag="PLR93_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26072.1"
                     /translation="SIREVLNVGNAVFCAAGNWELVIGNWRSEIESLLVDEPRRLALG
                     KQARLDVEQLTWAKREEQIMKAVQKNS"
BASE COUNT         1474 a         1304 c         1298 g         1370 t
ORIGIN      
        1 cggcatgcgc gtcggcgggc agaagacatt cccaatgaaa tttgggaata tgcaaatgtg
       61 gatgtaaagg tggggaagcc aagcagctgc aacatgccaa aatagccaat gaccgcacca
      121 acattgatca acccggcgcg taacaggaag agggcatggg tcagcccgcc tgcaaaggca
      181 agcccaagca gcaacatggc gatataacgc gcttcaagat cgccttgttg tacgaatgca
      241 tcgcgtacct tgcgggcatt ggttacaaaa cgatcgactt cggcttcctc ctgtgctgaa
      301 cctttcatga tctcgacacc atcaagcgac tcggagaggt gcgtattcat ttgcccgaac
      361 gtggcgcggg cagtatcggt caccggtgag agcaccttaa gatagcgtac caacgccaca
      421 aataaattgc gatgaatatc aacggtgtca acaccaacga tgggtgatag cgcggaccga
      481 agaacaaagg catcaaaata aacatgaatg agcccaccac caggtttacg cctgggctga
      541 acatgtaatt cacctcgcgc acatcattgg ttgcgcgcgc catggtgtcg cccactggct
      601 gcaagttgtg aaaggtcatg ctcttgccaa gcagggaaag atatagctca tcacgaatat
      661 cgcgttccat tttttgggca agcagctcgg cgccaaagtt acgccccagc tgcaatacgc
      721 cgcggatcac ctgcgaaacg ccgatggtaa tggcaagcgg cagcagcact gaagtatccg
      781 gctgtggtgc aagcatggca ttgaaggcat ctcctgtaag cacaggtacc acaacagcca
      841 gaaccgcatt accaatcgcg ccagtgacca gcatgatcat gatccaccaa tagggagaaa
      901 ggtgcgaaaa gatccaacgc actggcgaag aatggtcgta cttgactttt ctatgtaagg
      961 taaattcggc tggaatatca agggaggtca tttgataatt gtatcacccc cgtttcgtaa
     1021 catttaattc agacaagtgt tatccatatt aaccggcggt cctgccggaa tttagcgata
     1081 ataggagcaa tgaaaatgaa cttaccgtct tccctggcaa gttggtgtat ttggctattc
     1141 tttctttggt atggcttatc tgttttcata cctgccttga acaaggacct gtttagaaaa
     1201 atatccgggg tccttgcact gggtgtgttt gtgttcaatg tgttgggatt gtaattttta
     1261 gctggccgtg ccgcctccca aacgggaggc ggtgtcattt aagtcctgcg aaaatttctg
     1321 ccgcttcaat ggcatagccc gggtcaaggt caagatccca gcaggcggaa aggatcgaat
     1381 gagccaatgc ccatgcgatg atcttctcgc gctcccaacc gagtcgttca tggaaaatat
     1441 ccacccgcct cgccgcctga accttgaacc tgctccagtc catcgacatg ttccacgggt
     1501 tgagcatgaa cggaccgatc tcatagccca ttggcccaat aactcccttg gggtcgatca
     1561 ccagccagcc gcgttcggac gagagaatgt tgaaatgatg aaaatcgccg tggagtagaa
     1621 agttgccttc acgtgcaaat agatctggca aagagtcttc gactcgctcc aagagtcggg
     1681 ctgggatggg tcccgtaccg ccattgaaac gcgggcggat ggtctttaac tcgtcgagcc
     1741 aatcggtgag tggaatgaac ctttggagtc cggcagatcg ctgccggaca tcagacagca
     1801 agctgtccga ctccatatcc acaggccgac aggtcttttg cattacttcg actgcaatgt
     1861 gcgtgcgctc gtcgtcatct tccaattcgg aaagcatttt ccccggtttg aggcgttcga
     1921 gcaggaacat ccctttttct tcatcagatt ccagcaactg gcagatgccc tctccattga
     1981 agaacttcaa tgtgttcatc tcactaagca attcccggtt gagtactcca attttgagga
     2041 tgacatcctc gctgccgcgc ttggcatagg ccacaaagtt gtaagaaagg ttgggaacag
     2101 gctcaatagc cgttatgccc cagcgttggg aggattcgtc gatcaacatg gggagatcgg
     2161 cgagaagttg ttcgcctttt tgttcaaaga cggagcggat gaggttggtg aaggattcag
     2221 ggagttgcaa cgtcatagta ggaggtgaca gccactttga ggttctattc tttgaatttc
     2281 accaaatata caccgacaac caccatccat gcgagccaga gcaaactgcc aacgagaccc
     2341 gcccaatcca caacggggaa ggtggggatg gcagtggcca gtagctccgt ttgtgcgagc
     2401 agataaaccg cagaagcaac ccagccaagc acggcgaccc atttgctgaa catctttgat
     2461 cgataaatga tgctgctgag catggacatc caaaggatgg tgaggagctg tcccatatgt
     2521 tcgccgagaa tgactccgcc gtattgatgg actgcggtga acacagcgga gactgcggcc
     2581 tgggtcgctg aatcagttcc cggcgcggta tagaggcggg cgaggacggg cacaacgaag
     2641 acccagcgca ggagtccgat cacctggacg atgaagccca tcacaccgag agttgttgtc
     2701 agttccaaga agggagcatt ctcatctttg agaatctgtt taagcatgat gacgccaaag
     2761 agcatgggca gacccaccca agcgaaggcc agccaggtat agatgagtgc gttgccgcct
     2821 gcttgaaagc gggtgagaat ttcagcggtg ggttggcgca ggatgtcggg gtaatcgaag
     2881 ttgatgatga gcagggtgta ggggatgttg atcagcaccg taccgacaat gaagagcaga
     2941 ccagcaagtt gacgaaattg tcttgttgac atttgaattc ctttaaacaa gataaggttg
     3001 taatactcga agcatgagtt tcacgccgtt ttgcatttcg cccttattca cactgcgccc
     3061 attgacctca agattggtga gccagaattc gctaattaac cagacgatct cttgaagttc
     3121 atggatggct ttcggatcat cgggtaaatg gataatgcca gcatcagaaa acgtatggat
     3181 cagttcgtga aatccattaa atccacgctg gcggactgcg agaaagttct tacgtagttc
     3241 cgtgtcctga cgcagaaggg caaccaattc gcgataggca aagcgatatt gccagatgat
     3301 ctggtaattg gtttcaatga ttgtttttag atcggctagt gttggctgat gatcgtgtgg
     3361 gaacggcaaa ccgttgtccc atgtgtcaaa taaacgctgg aataatgcgc ggatgatctc
     3421 ttccttgttg cggaagtgat aatagaggtt gcccgggctg atgcccgccg cctcggcaat
     3481 gtgattggtg ctgacggcag cggttccctg gtcgttgaag agggttaggg cggtgtcgag
     3541 aattttttca cgggtggaca tgcttgctcc tagagtattt gctctattag tttagagtaa
     3601 atactctagt ttgtcaaggg cgaaaaatgc agatttgtct gctgatcatt cttgttatat
     3661 gattatggtt gcgaatgaat cttcaacctg gttccccaca acgatcttac ggaactgacg
     3721 aaggaataat atccatgcaa gacttttcac ccatccaaac caaacgactg tctctgcgcc
     3781 cgtttcaagc agatgacctt gacttcagct ttcaacattt cagtgatccg caagtggcaa
     3841 aatatctatt ggatgagcca cccgtcacag aactgtcaca ggcgcaggaa atcgtgcagt
     3901 tctatttgga ctctgcgggc aaaccgtaca atcgctgggt aatcgtctcc aatgcagacg
     3961 gtcaacgcat tggcacctgc ggattccaca aatggaacaa gccccaccag cacgctgaga
     4021 tcggctacga cctcagcccg aaggcgtggg gacaaggcta tatgcaagag gcgctatccg
     4081 cggcgattca atttgggttt atgcaaatga aacttcatcg tattgaagcc ttggtctacc
     4141 ccgaaaattc acgttccctt cacttgctgc ataaattagg cttcaaacag gaaggcatct
     4201 tgcgcgatta tttttatctc gatggcaagt tttacgacca tgtgatattg tctcttctaa
     4261 aaacatagaa aaactcaaac ttaattattt acaggagaga tctctttggt agtgacatcc
     4321 agccaccact gtttgttatc ataatcttct aagaggatct tatcctcaat atcccatttt
     4381 acaggctcgt aactttcttc gctattttcg attagaattt tttgataatt cgattctgga
     4441 aatacttgaa tgatataaga tgccggataa ataaaatcac ataccccgat gtagtaagca
     4501 taaacaagcg aatctccttc aggcgaccaa tatatatcgc ttgtatcatt ataataacct
     4561 tgagcatctt ctaaatcagg cagagggaaa gaggcagtaa ttcctttttg aatatcaagc
     4621 actgaaacat taccaccata catatcaacg aaggctaatt tttcttcgtc aggggataaa
     4681 gccatccacg ttgcactatc ttcttttatc actacaaaaa tacctagaag taagtcaaat
     4741 tttattaaat cagacccacc cggcttaaag tgtccaaaac aaccatctcc tcccattgac
     4801 aagtgtgaat agtacatata acgcccatct tttgaccaca taaaaacata cgggtactta
     4861 aaaccattca atggattatc tggctctaaa aagttgatat cttcaagtgt ccatttttgg
     4921 ttattaatat tattcacgac aataaaatta acattcttct gtgtgtcacg tgtagtaaca
     4981 cttacaacgc ctgtccattc tccatttggg gacatattct cttcaaagac aactgtttca
     5041 gcaactggtg tatttgtaaa aacgggagtt acagtaggaa ccgatgcagg tgtaaaagtc
     5101 ttcgttaact caagttgagt tggcttattt ggagtcacct ggcaactcat caagacaaaa
     5161 acaaaagaaa gacttaagag ttttatattt ggcaacattt ttattcactc cctttctgaa
     5221 tgcctgcgct taagaatttt tctgaaccgc tttcattatt tgctcttcac gcttggccca
     5281 tgtcaactgt tccacatcca agcgggcttg ttttcccaac gccaagcgac gcggctcatc
     5341 cacaagcagc gactcaatct ctgatctcca attgccaatt accaattccc aattccctgc
     5401 cgcacaaaac accgcattgc ccacattcaa cacttcccga atcgaa
//
LOCUS       DAOQFT010000092         5431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7654_length_5431_cov_7.498698, whole genome shotgun sequence.
ACCESSION   DAOQFT010000092 DAOQFT010000000
VERSION     DAOQFT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7654_length_5431_cov_7.498698"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="PLR93_03590"
     CDS             <1..1703
                     /locus_tag="PLR93_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNH26073.1"
                     /translation="PRFFVINKMDRENADFQKTYDAITEFVEAHGKRVVQVHIPIGEK
                     QNFKGVVDVIGMKSYMGDGKTIAEIPAELKEAADKAHFAMVEDAAEGEDQLMEKYLEN
                     GSLSDEEMVRGLEDVVYAGQFVPVFCAAGAHEIGTIALLNDIIDLIPSPAQAVKRMAQ
                     GKDGAEELKATDQGPLAAYVWKTTADPFVGKMTYFRVLSGSLQADAHVWNQNKGADER
                     MSGMHFQRGKETIAAKSIHAGDLAVVSKLTVTATGDTFCDKGHPLTVEKPAYPAALYR
                     VAVAPKTQADAAKISTVLTRLSEEDMTLTWQNDPVTHETVLQGMGDQHIDVAVHRAQN
                     KFQVGIVTHEPKVPYREGITRKAQAQYRHKKQSGGAGQFGEVHLRIEPYMEGDFDFVD
                     ALVGMNLSRSYLPPIEKSIRATMERGAFAGYPMSNVRVIVFDGKEHEVDSKPMAFEIA
                     GREAFKLAVHDAGPVLFEPIMTVRVTCPDANMGDVMSDLNTRRGRVQGTESERGNTTI
                     IAHVPMSEMLKYTTQIRSITGGRGYFTMEFDHYDTVPTHIAGPIIEAHKKEMEAKKEE
                     "
     gene            complement(1764..2339)
                     /locus_tag="PLR93_03595"
     CDS             complement(1764..2339)
                     /locus_tag="PLR93_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26074.1"
                     /translation="MIKRLLPAIPFIVLIACSPDISDPGVQAAVIHSLTATVWTPTPI
                     TPSPTPEPNTAKVVELLNDAMVGADPLMETIAAKYSVIDAQVIVDTNTHQAVSLQVYV
                     DCQWIYTDGCTPENTFVILVRAFSVNNKIMGKIQEQIPPTIQTLEVLTFEQMTKTSLI
                     HIAWKDICDYTEGRINGNQLGSRMIRFAVSP"
     gene            2514..2786
                     /locus_tag="PLR93_03600"
     CDS             2514..2786
                     /locus_tag="PLR93_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNH26075.1"
                     /translation="MPIVKFPPLMKFYVENQAEFEMQAKTVAELLENILVRYPILKTH
                     LFDSQGELRRHFNIFVNGVHVRELNGMETPLKEEDKVILMASAAGG"
     gene            3048..3998
                     /locus_tag="PLR93_03605"
     CDS             3048..3998
                     /locus_tag="PLR93_03605"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNH26076.1"
                     /translation="MTLLDFRLTESNTIPLDSLAYHVGNTPLLPLRRVTSNLSPRVRV
                     FAKAEWFNPGGSVKDRPALNIIQNAILNGNLSGGKRLLDSTSGNMGISYATFGAALGI
                     PVTLAVPASASAERISILKALGAELVLTDPLEGSDGAILKAREMVEEDPDKYWYANQY
                     NNEANWQAHYVSTGPEIIRQTNGQVSHFVAGLGTSGTFTGTSRYLLEQMPDVKTIAFQ
                     PDAPFHGLEGLKHMPSAIKPGIYDEALAGTPLEVKTEAAHEMVIRLAREEGLFVGISS
                     AAAAVAAIQVASELDEGVVVTVFPDAGYKYLSDKTLWEKA"
     gene            3999..4412
                     /locus_tag="PLR93_03610"
     CDS             3999..4412
                     /locus_tag="PLR93_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNH26077.1"
                     /translation="MSLKLTNEQVSEIERHIQAAYPGEGAGFLLGVDGEVKGVLPLDN
                     AREEEARYNRFLLTPEDYLKAEMKAMELGVDLIGVFHSHPDCPNVPSEYDREWAQPFF
                     SYIISRVDQGKTVSHRSWRLQEDRSKYDEEQIEVL"
     gene            4494..4730
                     /locus_tag="PLR93_03615"
     CDS             4494..4730
                     /locus_tag="PLR93_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNH26078.1"
                     /translation="MDVIKEDQVLDCTGLLCPMPIVKTKKAIDVLESGQVLKMIATDA
                     GSPPDIAAWSRQTGNELLLSTEEGGKFVFFLKKA"
     gene            4740..5264
                     /locus_tag="PLR93_03620"
     CDS             4740..5264
                     /locus_tag="PLR93_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNH26079.1"
                     /translation="MAKSDPNAPKRLALIASKGTLDWAYPPFILSTAAAAMGWEVGVF
                     FTFYGLTLLKPELTAAVSPLGNPAMPMKMPFGPENFQNISWPMPNLLMANVPGFESLA
                     TNLMKQTFKNKGVASIEELRDMAVELDVRLIGCQMTMDVFGFKKEDFIPQAEIGGAAT
                     FLEYAADADVQLFI"
BASE COUNT         1430 a         1175 c         1430 g         1396 t
ORIGIN      
        1 tgccgcgttt ctttgtgatc aacaaaatgg atcgtgaaaa tgcggatttc caaaaaacat
       61 acgatgccat cactgaattt gtggaagcgc atggaaaacg cgttgttcaa gttcacattc
      121 ccatcggcga gaagcaaaat ttcaaaggtg tggtcgatgt catcggcatg aaatcgtata
      181 tgggggatgg gaagaccatc gccgaaatcc ctgctgagtt aaaagaagct gcggataagg
      241 ctcacttcgc tatggtggaa gatgccgccg aaggtgaaga ccaattgatg gaaaaatatc
      301 tcgagaacgg ctctctctca gacgaggaaa tggtgcgtgg tctcgaagat gtggtctacg
      361 ccggacaatt tgtaccggtc ttttgtgcag cgggtgcgca tgaaattggc accattgctt
      421 tgctcaatga catcattgat ttgatcccat cacccgctca ggctgtgaag cgtatggcac
      481 aaggcaaaga tggcgctgaa gaattgaaag ccacagacca aggtccattg gctgcttatg
      541 tttggaagac caccgccgac ccctttgtcg gaaagatgac ctatttccgc gtgctttctg
      601 gctcgctaca agcagatgcg catgtctgga atcaaaacaa gggcgcagat gagcgcatgt
      661 ctggtatgca cttccagcgc ggaaaagaaa cgattgctgc caagagcatt catgcgggtg
      721 atcttgcggt cgtttccaag ttgaccgtca ctgcgactgg cgatactttt tgtgataagg
      781 ggcatccact cacagtggaa aaacctgctt atcctgcagc cttgtatcgc gtggcagtag
      841 ctcccaagac ccaagccgat gcggcaaaga tttctaccgt gctcacccgc ttgagcgaag
      901 aagacatgac cctcacttgg caaaacgacc ctgtgactca tgagaccgtc ttgcaaggga
      961 tgggggatca gcacatcgat gtggcagtac atcgcgctca aaataaattc caagtgggca
     1021 tcgttacgca tgagccgaag gtcccatatc gcgaagggat tacgagaaaa gcccaagcgc
     1081 aatatcgcca caagaaacaa tcaggtggtg caggtcaatt cggtgaagta catcttcgca
     1141 ttgaaccgta catggaaggt gattttgatt ttgtagacgc cttggttggt atgaacctct
     1201 cgcgttctta cttaccgccc attgaaaaga gcatccgcgc tacgatggaa cgaggagcct
     1261 ttgctggtta ccccatgagc aatgtgcgtg tgatcgtttt cgacggtaag gaacatgaag
     1321 tagactcgaa gccaatggca tttgaaattg ctggacgtga agccttcaag ctggctgtgc
     1381 atgatgctgg accggtattg tttgagccaa tcatgaccgt gcgtgtgacc tgtccggatg
     1441 cgaatatggg cgatgtgatg agtgacctca acacccgccg tgggcgtgta caaggcaccg
     1501 aatcagagcg tggcaacacc accattattg cgcacgtgcc gatgtctgaa atgctcaagt
     1561 acactacaca gatccgctct atcacaggcg ggcgtgggta tttcaccatg gagttcgacc
     1621 attacgatac ggtgccgacc cacattgcag gtccgatcat cgaagcccat aagaaagaaa
     1681 tggaagccaa gaaggaagaa tagaaaagaa agattgaagt atgaagagtt ccgggttttg
     1741 cccggaactc tttttgtttg gaattaggga gagactgcga aacggatcat acgagacccc
     1801 aactgatttc cgttgatgcg accttcggtg taatcacaga tatctttcca cgcaatgtgg
     1861 atgaggctgg ttttcgtcat ttgctcaaat gtcagaactt ctagcgtttg aattgtggga
     1921 ggaatttgtt cttggatttt ccccataatt ttgttgttca ctgagaaagc acgcaccaag
     1981 ataacaaacg tattttcggg tgtgcaacca tctgtataga tccattgaca gtccacatag
     2041 acttgtaatg agactgcttg gtgcgtattg gtatcgacaa taacttgagc atcaataaca
     2101 ctgtattttg ctgcgatcgt ttccatgagc gggtcagcac caaccatggc gtcatttaaa
     2161 agctctacca ctttggctgt gttcggttca ggggtagggg atggagtgat tggtgtagga
     2221 gtccatacgg ttgccgtcaa cgaatggata acggcggctt gaacgcccgg gtctgatata
     2281 tctggcgagc aggcaataag gacaataaag gggatggctg gtaacaaacg tttgatcata
     2341 gaacctcctt tgtgcaggtt ttacacccgt gcccacatca acgtcaattc cgtttgttat
     2401 acgatgcacc tacgctcact caacgatgct ctcatatata taatttattt ttaaatgtaa
     2461 atatgcagac ctgcctctta catagtgaaa agaacttcgt gtagaataca aatatgccaa
     2521 ttgttaaatt ccctccactc atgaagtttt atgtagagaa tcaagctgag tttgaaatgc
     2581 aagcaaaaac cgtggctgag ttattggaga atattcttgt ccgatatccc atactcaaga
     2641 ctcacctttt tgactctcaa ggggaacttc gccggcattt caatattttt gtgaatggcg
     2701 ttcatgtgcg agagttaaat ggaatggaaa ccccattgaa agaagaggat aaggttattt
     2761 tgatggcatc tgctgctggg ggatgaattc ccaaattatc ttgacaaaat cggtaagatt
     2821 catagaatag gcgcaatcta ggtaacagaa aaatatgaaa cgtacagtat attgcttgtg
     2881 tcttgggcgg ggctcgtagc cgccgcctga gttaatatcc gcagtcaacg gaatcagacg
     2941 gtagagcgac ccccgcgtgc atcgaatttg catcgtgtgt ggcgtttgtt cgccgtcttt
     3001 ttgtttatcc gttggctgtt tttattttaa tcagagaaga gaagaaaatg actttattag
     3061 actttcgatt aaccgaatcc aatacgattc cgttagacag ccttgcttat catgttggga
     3121 atacgccgct tcttccgctt cgccgcgtga cctcgaacct gtctccgcgt gtgcgagtgt
     3181 ttgccaaggc tgaatggttc aaccccggcg gttcggtcaa agatcgccct gcattgaaca
     3241 ttattcagaa cgccatctta aatggaaatt taagcggcgg caaacgtttg ctagattcca
     3301 cttctggcaa tatgggtatt tcgtatgcga cctttggtgc cgcattggga attccagtca
     3361 cattggcggt gcccgccagc gccagcgccg agcggatttc catcctcaaa gccttgggcg
     3421 cagagttggt tcttaccgac ccgctcgaag gttcagatgg agcaattctc aaagcacgtg
     3481 aaatggtgga agaagatcca gataaatatt ggtatgccaa tcaatataac aacgaagcga
     3541 attggcaggc acattatgtg agcacgggtc cagaaatcat tcgccaaacg aatggacaag
     3601 tctctcactt tgtagcaggt ttggggacct ctggcacgtt cacgggtaca agccgttatc
     3661 tcttggagca gatgcctgac gttaagacga ttgctttcca accggatgca cccttccacg
     3721 gtttggaagg gctcaaacat atgcctagtg cgatcaagcc tgggatttat gatgaggcgc
     3781 tcgcgggcac tccgcttgag gttaagacag aggcggcgca tgagatggtg attcggcttg
     3841 cgcgtgaaga aggtctgttc gtgggaatct catctgcagc ggcagcagtg gctgcgattc
     3901 aagttgccag tgaactggat gaaggtgtag tggtgacggt cttcccggat gctgggtata
     3961 aatatttgag cgataaaaca ctttgggaga aagcgtaaat gagtttgaag ttgacaaatg
     4021 aacaggtaag cgaaattgaa agacatattc aagcggcata cccgggggag ggcgctggct
     4081 ttttgctggg cgttgacgga gaagtgaaag gggttttgcc tttggataat gcacgtgaag
     4141 aagaagcgcg ctataaccgt tttttactta cacccgaaga ttatttgaaa gcggaaatga
     4201 aagcgatgga gttgggtgtg gatttgatcg gtgtgtttca ttcgcacccc gattgtccga
     4261 acgtcccttc ggagtatgac cgtgaatggg cacaaccatt tttctcttac atcatatccc
     4321 gagtggatca agggaagacg gtcagtcatc gctcgtggcg tttgcaggaa gatcgttcaa
     4381 aatacgatga agaacaaata gaagtcctgt aggggcgacc cgacagagtt tattgggcac
     4441 cttgctttca ctgggtgggg cgcccttaca atcatatgag gagaattttt aacatggacg
     4501 tcattaagga agaccaagtt ttagattgca cgggattatt atgcccgatg ccaattgtga
     4561 aaacaaagaa agcgattgat gtattggaat ctggacaggt tttgaagatg atcgctactg
     4621 atgcaggctc gccgccagat attgctgcat ggagccgcca gaccgggaat gaattgttgc
     4681 tttcgactga agaaggtggc aagttcgttt tctttttgaa gaaagcgtag ggtaaagaga
     4741 tggcaaaatc agacccgaat gctccgaaac gactggcatt gatcgccagc aaaggcacct
     4801 tagactgggc atacccgcct tttattcttt ccacggctgc agctgccatg ggttgggaag
     4861 tgggtgtgtt cttcaccttt tacggattaa ccctgctcaa gcctgaactg actgccgcgg
     4921 tctctccgct tggtaaccct gccatgccga tgaaaatgcc gtttggtcca gaaaactttc
     4981 agaatatttc atggcccatg cccaatttgc tcatggcaaa tgtgccgggt tttgaaagct
     5041 tggcgaccaa tttgatgaag cagaccttta agaataaagg tgtcgcttcc attgaagagt
     5101 tgcgtgatat ggcggtggaa ctggatgtgc gtttgatcgg ttgccagatg acgatggatg
     5161 ttttcggctt caaaaaggaa gatttcatcc cgcaggctga aattggtggg gcggcaactt
     5221 tccttgaata tgcagccgat gcggatgtac aattgtttat atagagcttt ttctgtcatt
     5281 gcctgtaccg tgcgcaggca cgcgtgcgag ggcgtttttg ctctttgcac aaagcaatcc
     5341 cgttactcat aattggattg cttcgccgcc taaaagcaag agcggcggct cgcaatgacg
     5401 ggtaaatgaa aaaaataaaa cgagagagaa a
//
LOCUS       DAOQFT010000093         5401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7725_length_5401_cov_6.482791, whole genome shotgun sequence.
ACCESSION   DAOQFT010000093 DAOQFT010000000
VERSION     DAOQFT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7725_length_5401_cov_6.482791"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLR93_03625"
     CDS             complement(<1..675)
                     /locus_tag="PLR93_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26080.1"
                     /translation="MTIESDSKPVRKTLGLTGITINAMALTAPGAFLWLLYQVQASAK
                     FDGVADIWPGVLLALLSAALTALSFGELARKYPEAGFRSAYHFADRFFARHKNSKHKS
                     VARLGKFAIGWAAHLYYWIYPGVLIAFMGILADYLLRYFGYQPTVFGDALLAASFAAF
                     AGFLALRGITGTTTTSVILNTIQLLTLLTFGILAITFRLITPAAFAPTEWAYPSALAV
                     LIPHSFD"
     gene            complement(665..907)
                     /locus_tag="PLR93_03630"
     CDS             complement(665..907)
                     /locus_tag="PLR93_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26081.1"
                     /translation="MHETTSNISPPELIYLGMGLMIARILLDGTNTRGFTWLQWLQFL
                     VNGARIVVLWPLVLFIEKFEAWLKSGSSDDEVKHDH"
     gene            complement(1013..2557)
                     /locus_tag="PLR93_03635"
     CDS             complement(1013..2557)
                     /locus_tag="PLR93_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNH26082.1"
                     /translation="MKALNKRKLKFVLPLLVMLLAFVLVTPAFAQDGNPPTTEQVDDI
                     ALGVNMLWMLLGGFLVFFMQAGFALVETGFTRNKNVVHTMMMNMMVFCIGAVGYWLTG
                     FAFQFGGVNAAYPEVSTVGAIAGEWAHSPITLGDWSGLLATPLLRFGQFGLLGGSGFA
                     LAGVEANAGILAFFLFQMVFMDTAATIPTGSMAERLKFSGFVVMGLWVSMFIYPLVAG
                     WIWGGGWLQNLGRIAGLGNGAVDFAGSGPVHMIGGCVGLAGAIVLGPRIGKFNKDGSA
                     NSIPGHNITIGVLGTIILFFGWFGFNPGSSLGFLGGFRNLAVIAAINTLLAGAAGGVS
                     AMTYMWLFGPTKKPDAGMSVNGILAGLVAITAPCAFVAPWAAVVIGTIAGVLVIWAST
                     ALEKLKIDDPVGAVPVHMVNGAWGVLAVGIFANGNPDTAAWNGMESAVTGLIYGGTTQ
                     VIAQLAEVVSIFVVVVGLSWVFFKAMYALKLMRVSREDELAGLDLPEMGSLAYPVDWE
                     APADAV"
     gene            complement(2581..3051)
                     /locus_tag="PLR93_03640"
     CDS             complement(2581..3051)
                     /locus_tag="PLR93_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26083.1"
                     /translation="MYALSIVGYIMIALLLMNMMAKARPQIKEPMPGKALKSLPMGLA
                     SRFRLQRSSIMALLTGIVFGVVTGWLSLSTAGIVIAVALATIFLPMQYTFTTKGIALG
                     QGMFYPWSDFSGFTSKGSTLQFERSSVFGGLTVSIASTEVDTVLKYAERYIETR"
     gene            complement(3075..4379)
                     /locus_tag="PLR93_03645"
     CDS             complement(3075..4379)
                     /locus_tag="PLR93_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNH26084.1"
                     /translation="MIFTIIIFLLAYIAIASEKFPRHWVALLGGSLLILFGVLTPSEA
                     LSYISWETLGLVAGMFTLVSILVNAGFFTWLAMTSLRKVNYHPVPLFIILILLAAFLA
                     AFMDSITVMLFLSALTLQLCTLLKIDPVPLVIAEVCAANTGGAATLVGDPPNVILGTT
                     LGFSFADFATNTGPIAIVGALILMAYAYIKNRKSLKEAHIALTPEMIDQIDELHNEPL
                     HVHLTRVGLTGFMMAVILLIFHIPLSTLTGLPINAAVSALIPAGIALIALRDDQRKKV
                     IISVDGESILFFAGLFMLIGGLEKVHLFETLAESLALATASNPNSLIMALHWGPGLAS
                     GILDNVPLALAMTFVLKDLAKIPGTPALSMMVWSLAMGVDVGGNLTPIGASANVVAYS
                     YIEHNHGNIGWKRWLAIAVPPTLIVMIVTSIILLYKYQIGWY"
     gene            complement(4610..>5401)
                     /locus_tag="PLR93_03650"
     CDS             complement(4610..>5401)
                     /locus_tag="PLR93_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26085.1"
                     /translation="GHGGMGGNRETWRLPMQDVETDAVPRTNLHFTSSSVPGNGLGAY
                     NADPVHILVEGGAQDGAAKGISGGRVVIMKGYNHNGILIDGSVGKSLAYGGTGGVVVV
                     QGNADSRACVRLSGADVIIGGEINKPLNDSLGFMGARANVKGFLCEYMTAGRVLVLGD
                     PGPWMCAGMTGGVLYLRLQPHLHFDYPAIQRRLAKGAKVSVQAVDDADFQNLQHLLTI
                     YAEELRQNHQADEADNALGLLIDWKNTFIRVVPEGLQEDQRFATE"
BASE COUNT         1527 a         1327 c         1367 g         1180 t
ORIGIN      
        1 gtcaaagctg tggggtatga gaacggctaa ggcggaggga taggcccatt ctgtgggggc
       61 aaatgcggcg ggggtgatga ggcggaatgt aatcgcaaga atgccgaatg tgagtagagt
      121 caataactga atggtgttca agatcacgct ggtggtggtg gtgcctgtaa ttccgcgtaa
      181 tgcgaggaaa ccagcaaagg cagcgaacga agcggctaag agtgcatctc cgaatacagt
      241 aggttggtac ccaaagtaac gcagtaaata atcggcaaga atgcccatga aggcgattag
      301 aacgccgggg tagatccaat aataaagatg cgctgcccaa ccaattgcga attttcccaa
      361 acgtgctaca gacttatgtt tggaattttt atgacgcgca aaaaaacggt ctgcaaaatg
      421 gtaagcgctt cgaaaccccg cttcaggata ttttctagca agctctccaa atgaaagggc
      481 tgtgagtgct gctgaaagta atgccaatag aaccccgggc caaatatctg caacaccatc
      541 aaacttggca gaggcttgta cttggtagag cagccataag aaagcgccgg gcgcagtcaa
      601 cgccatggca ttgatcgtaa tccctgttaa cccaagtgtt ttacgaactg gcttggaatc
      661 ggactcaatg gtcatgcttt acctcgtcgt ctgatgaacc tgatttcaac caggcttcga
      721 atttttcaat gaacaatacc aggggccaga gcacgacgat acgtgctccg tttaccagaa
      781 actgaagcca ttgcagccag gtgaatccgc gtgtgttggt gccgtctaac aagatgcgcg
      841 cgatcataag ccccatgcca aggtatatta attcaggtgg agagatattg gatgttgtct
      901 catgcatgac aatgctgtac tctatcttga aattggtatc gagactccgg aaggcggcga
      961 tgtaattacc cttccgaagt ctcgatgttg aaattactgg tgagtataga gactatactg
     1021 cgtcagcagg agcttcccag tctacagggt aggcgagaga gcccatttca ggaaggtcga
     1081 gacctgccaa ttcgtcttcg cgagaaacac gcatcaactt gagggcgtac atggctttga
     1141 agaataccca tgagagaccc acaacgacca caaagatgct gaccacttca gccagctggg
     1201 ctatgacctg agttgtacca ccatagatta aaccggttac ggcgctttcc atgccgttcc
     1261 atgcagcggt atcggggttg ccattggcga aaatgccgac ggcgaggacg ccccaagcgc
     1321 cattgaccat gtggacagga actgcaccga cggggtcatc gatctttaat ttctcaagag
     1381 cggtggacgc ccaaattacc aacacacctg caatggtacc aatcacaaca gctgcccaag
     1441 gagcaacaaa tgcacaagga gctgtgatgg caaccaagcc agccaaaata ccattgacgg
     1501 acatgccggc atctggtttc ttggtgggac caaacagcca catataagtc attgcagaaa
     1561 cgccaccagc ggcaccagcc aacagcgtgt tgatggcggc gataacggct aagttgcgga
     1621 agccgcccaa aaatccaagg gaagagccgg ggttgaaacc aaaccaaccg aagaacaaga
     1681 taatggttcc aagcacgcca atggtgatgt tatgtcccgg gatcgaattg gcggagccgt
     1741 ctttgttgaa cttaccaata cgcggaccca gcacaattgc gccagctaaa ccgacacaac
     1801 caccgatcat gtggacagga cccgaaccgg caaagtccac ggcgccattg cccaaaccag
     1861 cgatacgacc taagttctgg agccaaccac caccccagat ccaaccagca accaaaggat
     1921 aaatgaacat acttacccag agacccatta ccacaaaacc agagaacttg aggcgttcag
     1981 ccattgagcc ggtggggatg gtagcggctg tatccatgaa gaccatctgg aataagaaga
     2041 aggcgagaat accagcgttc gcttctacac cagccaatgc aaaaccactt ccaccgagaa
     2101 gaccaaactg accaaagcgt aacaaggggg ttgctaaaag cccgctccaa tcgccaaggg
     2161 tgatcgggga gtgtgcccat tcaccagcga tagcgccaac agtggatact tctgggtagg
     2221 cagcattcac accaccgaac tggaaggcaa agccggttaa ccagtagcca acagcaccaa
     2281 tacagaagac catcatgttc atcatcatgg tgtggacgac gttcttgtta cgggtgaagc
     2341 cggtttccac cagagcgaac ccagcctgca tgaagaagac caagaaaccg ccaagcagca
     2401 tccaaagcat gttgacaccc agggcaatat catccacttg ttcagtggtg ggtgggtttc
     2461 cgtcctgtgc aaaagcaggt gttacaagca caaaagctaa taacatgacc aacaaaggca
     2521 aaacaaactt tagctttctt ttattcaatg ctttcatttt cattctcctc ttttgagttt
     2581 ttatcttgtt tcaatatagc gttcggcata cttcaaaaca gtatccacct cggtggaggc
     2641 gatggatact gtaagacctc caaaaacgga agagcgttca aattgtagag ttgacccttt
     2701 gctcgtaaac cctgaaaaat cactccacgg gtagaacatt ccttgcccaa gcgcaattcc
     2761 ctttgttgtg aaggtgtatt gcatcggtaa aaagatggtg gcaagggcaa ctgcgatgac
     2821 gatccctgca gtgctgagcg ataaccaacc cgtaacgacc ccgaaaacta tccctgtcaa
     2881 caacgccatg atgcttgatc gctgcaaacg aaaccgggat gccagcccca tgggtaggct
     2941 tttcaatgct tttcctggca tgggttcttt gatctgtgga cgggcttttg ccatcatgtt
     3001 catcaacaac aaggcgatca tgatgtaacc aacaattgaa agtgcgtaca tgatttcttc
     3061 tctccttgaa aaaattagta ccaaccgatt tgatatttat agagcaagat gatcgatgtg
     3121 acgatcatga cgattaacgt aggcggtaca gcaattgcca accagcgctt ccaaccgata
     3181 tttccgtgat tgtgttcgat gtaggagtaa gcaacgacat tggcggatgc tccgattgga
     3241 gttaaattac cgcctacatc aacacccatc gccaatgacc aaaccatcat ggaaagtgca
     3301 ggtgtaccgg ggatttttgc taggtctttg agcacaaagg tcattgccaa agctagagga
     3361 acattgtcta gaatgccact ggcaagacct ggtccccagt gcaatgccat gatcaaggaa
     3421 tttgggttgg atgcagttgc cagagccaat gactcagcaa gagtttcgaa gagatggact
     3481 ttctccagcc cgccaatgag cataaagaga ccggcaaaga atagaatgct ttcaccgtct
     3541 acgctgataa tgactttctt gcgctggtca tctcttaagg cgatcagtgc gatgccagca
     3601 gggatcaaag ccgatactgc cgcattgatg ggtagcccgg ttagcgtgct taggggaatg
     3661 tggaagatca agaggatgac tgccatcata aaaccggtta gcccgacacg agtcaggtga
     3721 acatgaagcg gttcattatg caattcgtcg atctggtcga tcatctctgg tgtgagcgcg
     3781 atgtgagctt ctttcaacga tttgcgattt ttgatatagg catatgccat gaggatcagc
     3841 gcgccgacaa tggcgattgg accggtattt gtggcgaaat cagcaaaact gaaccccaat
     3901 gtagttccta gaataacatt aggaggatcg ccgaccaatg tggctgcgcc gccagtgttg
     3961 gcagcacaga cttctgcgat cacaagtggt acggggtcaa tcttcaacaa agtgcagagt
     4021 tgtaatgtca atgcagaaag gaacagcatt actgtaatgc tatccatgaa ggctgccagg
     4081 aatgcagcca aaaggatcaa aataatgaat aagggcacag ggtggtaatt gactttgcga
     4141 agagatgtca tggctaacca agtaaaaaag ccagcgttaa ccaaaatgga gacgagggta
     4201 aacatcccag caaccaaacc tagagtttcc cagctgatgt atgaaagagc ttctgatgga
     4261 gtgaggacac caaacaagat tagaagtgaa ccacccaaaa gggcaaccca atggcgtgga
     4321 aacttctcac ttgcgatcgc gatatatgcc aacaaaaaga tgatgatggt gaagatcaaa
     4381 ggggcatctc tcctcaaaag atgaataacg ttttatgtaa tgcgccttat tgtatggaat
     4441 tgaacctgta tggctattac cctaatgggt attcatttca tgttgtgctc aaacaactat
     4501 agatcacatt gaaaagatga cccaaatgga gcacgtttaa atgacaactg cgccatgaaa
     4561 caatttctca tggcgcagtt gatttaattt cacccgtttg ggtacgttgt tattcggtag
     4621 cgaatctctg atcttcctgt aaaccttccg gcacgacacg gataaaggta ttcttccaat
     4681 cgatcaacag acccagtgca ttatcggctt cgtcggcttg atggttttga cgtaactctt
     4741 cagcatagat agtgagcagg tgttgcaagt tctggaagtc ggcatcatcc accgcttgca
     4801 cagagacttt cgcgcccttg gcgaggcgac gttgaatggc ggggtagtca aaatgcaaat
     4861 gcggttgcaa gcgcaggtac aagacgccgc cagtcatgcc tgcacacatc cacggaccgg
     4921 ggtcaccgag cacgagcacg cgcccagcgg tcatgtattc gcacaggaag cctttgacgt
     4981 tggcgcgcgc gcccatgaag ccgaggctgt cattgagcgg tttgttgatc tcaccgccga
     5041 tgatgacatc ggcaccggag aggcgcacac aggcacggga gtccgcgttg ccttgcacga
     5101 ccaccacgcc gcccgtaccg ccataagcga gactcttacc aaccgagcca tcgatcagaa
     5161 tgccattgtg gttgtagcct ttcatgatga caacacgtcc gccggagatg ccttttgccg
     5221 cgccatcctg tgcgccgcct tcgacgagga tgtgaaccgg gtcagcgttg taggcaccga
     5281 gcccattgcc aggcacggaa ctgctggtga aatgcaaatt ggtgcgcgga accgcatcgg
     5341 tctcaacatc ttgcatgggc aagcgccagg tctcgcggtt tccgcccatg ccgccgtgac
     5401 c
//
LOCUS       DAOQFT010000094         5387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7761_length_5387_cov_5.589647, whole genome shotgun sequence.
ACCESSION   DAOQFT010000094 DAOQFT010000000
VERSION     DAOQFT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7761_length_5387_cov_5.589647"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="PLR93_03655"
     CDS             <1..1294
                     /locus_tag="PLR93_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH26086.1"
                     /translation="ADLIYYSCYQMEKNNGYIAEMGRDPLVGYESRNVSVLRPYGVWL
                     VVSPFNFPHALTGGPSGAALAAGNTLVIKPATDTAWIVRLLVDCFRDAGFPEGVVNFV
                     TGPGSTLGQALVDSPEVDGVTFTGSFDVGMKMYRDYANRNYVRPMILELGGKNPAIVS
                     RNADLETAAVGIVRSAFGLQGQKCSAASRVLVEEPVYDELVARLKDLTDKLVLGDPTN
                     RATYNGPVINKSSYQDFKDFTEEISQGGGKFLTGGHVRVDGDYGKGFFCESTFVTDLP
                     FEHRLWKHEMFLPITTIGKVQNLDQAMGIANDVNYGLTAGFYGNPAETEWFFDNIEAG
                     VTYANRPQGASTGAWPGFQPFGGWKGSGSSGKNAGGHYYLPLYMHEQIHTSVKVAKAA
                     PAKKAAAKKTVAKKVATKKVAAKKTVKKAVKRIAKKKK"
     gene            1348..1740
                     /locus_tag="PLR93_03660"
     CDS             1348..1740
                     /locus_tag="PLR93_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26087.1"
                     /translation="MRVIKQSFQIIHYLIFMVGILFISACSNELNTPTPVATENVPTF
                     NVQIPDKALLKNYLVVSLETEPNTTCELTYIAPKGQKLHMIETADENGICEWRWKLEE
                     SDGRGHGRLIFTINGVSETHFIDIRPGF"
     gene            1811..2884
                     /locus_tag="PLR93_03665"
     CDS             1811..2884
                     /locus_tag="PLR93_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26088.1"
                     /translation="MEQELKLRDYPIIGWLVGLGGLGWGGYTFFNTLPAFNLNAALVG
                     FGGLLFLVLSYSLVVTADKTTGLLILDYRSIFHHSTKEIPLSQISNIRVDRSRTRSSK
                     GGSSTTYRVEAILSSGEVVPFRSYHTGNLFKHQKWANELRAFLNISEGFDETPQGFFK
                     AAQQAGAMIAQRQQEALTGSNAAMRNTDGVNWQLQSFGIGATPGTRWYSPDFKTQGSF
                     LFLAQKIAGQASTGFLASLGKTLFKQSISLYGFDVSDTPNIEKADTYASLSAALDTHF
                     SAFTSNQTEAQQILNPWTQQPLADWGVKYPLQQFQSNARFSQLVVMFNPKGIYIATLG
                     TFPEDKLEEITRLGVELVKAHGS"
     gene            2990..>3200
                     /locus_tag="PLR93_03670"
     CDS             2990..>3200
                     /locus_tag="PLR93_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNH26089.1"
                     /translation="MYTPKPLKWDDALSTGNQTLDEQHKYLIDTLNSLGDAIIEGHGI
                     DAIARILGRLRFYAGWHFGREEECFE"
     assembly_gap    3201..3300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3301..3576
                     /locus_tag="PLR93_03675"
     CDS             <3301..3576
                     /locus_tag="PLR93_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNH26090.1"
                     /translation="RLRFYAGWHFGREEECFESYHCPAAEKNKKAHAVFIEKFDKYQD
                     KFHESGGSLDLALKVHEEISDWIVNHIMIVDGELYPCIHNHPKPLKK"
     gene            complement(3618..3890)
                     /locus_tag="PLR93_03680"
     CDS             complement(3618..3890)
                     /locus_tag="PLR93_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26091.1"
                     /translation="MQTDPASLDPEERLNKFYALLSAGFGVLSLCAGLIPMAGTALGI
                     AGIVFGLLGRRSENKRLAVVGIILSIIGVLTAIIYSILLYRAKYGA"
     gene            complement(3893..>5387)
                     /gene="uvrB"
                     /locus_tag="PLR93_03685"
     CDS             complement(3893..>5387)
                     /gene="uvrB"
                     /locus_tag="PLR93_03685"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNH26092.1"
                     /translation="DMELRPGTFRVRGDTLEIIPAYEDKKGYKITFFGDEVERIMQFS
                     PLTGEIYEEPQEISIYPAKQFLTDQERLKESIKNIETELEERLALYKSEGKFLEAQRI
                     EQRTRYDLEMLKEVGYCSGIENYSRHLDGRPAGSHPWTMIDFLPSDYLLILDESHMTV
                     PQIRGMYNGDRARKETLVEYGFRLPSALDNRPLKFDEFEQVMGSTIYTSATPADYEMK
                     HAEQVVEQIIRPTGLIDPEVIVRPVKGQVDDLVGEIKQRVERGERILVTTLTKRMAED
                     LTTYMKELGIKVNYLHSEVETLERVGILRDLRLGNFDVLVGINLLREGLDLPEVSLVA
                     ILDADKEGFLRSDTALIQTIGRAARNVNGRVIMYADRMTDSMKRAIDETNRRRAKQVK
                     YNEEKGIVPFSITKAIHDLTEEMSAKAVGEMKGEYKVKNKNEMPRNELRQVMHEMEKQ
                     MKDAAKNLEFEKAAALRDELYELKSLLAEDESLKPWERIKLLSGEEE"
BASE COUNT         1433 a         1373 c         1152 g         1329 t
ORIGIN      
        1 tgccgacctt atttattatt cctgctacca aatggaaaag aacaacggct acattgcaga
       61 aatgggacgc gacccgttgg tggggtacga atctcgtaac gtgtctgtat tacgccctta
      121 tggtgtgtgg ctcgtggtat caccgttcaa cttcccgcac gcgctcacag gcggaccttc
      181 gggtgctgct ctggctgcgg gcaacaccct tgtcatcaaa cctgcaactg atacggcttg
      241 gattgtgcgc ttgctagtag attgcttccg tgatgcaggc ttccctgaag gtgtggtcaa
      301 ctttgtgacg ggtcccggct cgactctggg tcaggcactg gtcgacagcc ccgaagtcga
      361 cggcgtcacc tttaccggtt cttttgatgt cggtatgaag atgtaccgtg attacgccaa
      421 ccgcaattat gtgcgcccaa tgatcctcga gctgggcggt aagaatcctg ccatcgtttc
      481 ccgcaacgca gaccttgaaa ctgcggctgt ggggattgtc cgctcggcat ttggcttgca
      541 gggacagaaa tgctcggcgg cgtctcgcgt gttggtggaa gaacccgtct atgatgaact
      601 ggttgcccgc ctcaaagacc tgaccgataa actcgtgctc ggtgacccaa ccaaccgcgc
      661 cacctataac ggacctgtga tcaacaagag ttcgtatcaa gacttcaaag atttcactga
      721 agagatcagt caaggcggcg gcaaattcct tacaggtggg cacgtccgcg tagacggtga
      781 ttacggcaag ggcttcttct gcgaatccac attcgtgacc gacctgcctt ttgaacatcg
      841 cctgtggaaa catgaaatgt tcctacctat caccaccatc ggcaaggtac aaaacctcga
      901 ccaagccatg ggcatcgcca acgatgtcaa ctatggtctg accgccggtt tctacggcaa
      961 cccagccgaa actgaatggt tcttcgacaa tatcgaagct ggcgtgacct acgccaaccg
     1021 cccacaaggt gcctccacag gtgcctggcc tggcttccaa ccctttggtg gctggaaagg
     1081 ctctggctca tctggtaaga acgcaggcgg tcactactac ctgccgctct acatgcatga
     1141 gcagattcac acttcagtaa aagttgcgaa agcggctccc gcaaagaagg cagcagcgaa
     1201 gaaaactgta gcaaagaaag tggctacgaa aaaagtagct gcaaagaaaa cggtcaagaa
     1261 agccgtaaag aggatagcta agaaaaagaa ataatctgaa attagcctct cgagtacgct
     1321 cgggaggctt tttgatagaa ttctcctatg agagtcataa aacaaagttt tcaaatcatt
     1381 cactatctta tttttatggt tggtatattg ttcatctccg cttgtagcaa tgaacttaac
     1441 acaccgactc ctgtagccac agagaatgtg ccgaccttta acgtccaaat cccagataaa
     1501 gctctcttaa aaaattatct cgttgtcagt ttggaaacag agccaaacac aacctgtgag
     1561 ttgacctaca ttgcccccaa aggacaaaaa cttcacatga ttgaaaccgc tgatgaaaac
     1621 ggaatctgtg aatggcgctg gaagcttgaa gaaagcgatg gcagagggca tggacgtctg
     1681 attttcacca tcaacggcgt cagcgaaacc cactttatcg acatccgccc ggggttttag
     1741 ataggagctt tgtctggttt gtatccattg aaaatttctt ataattaaat aaaaacagag
     1801 gagcaacata atggaacagg aactcaagct tagagattat ccgatcattg gttggctggt
     1861 agggcttggc ggactcggtt ggggtggcta tacctttttc aatacattac ctgctttcaa
     1921 cctgaatgcc gccctagtgg ggtttggagg gctacttttc ctagtcttaa gttactcact
     1981 cgttgtcact gccgataaga ccaccggctt actcatcctc gactatcgct ctatttttca
     2041 tcattccact aaagaaattc cgctcagtca gattagtaat atcagagtag accgttctcg
     2101 cacacgcagc agcaaaggcg gatcaagcac aacctatcgt gttgaagcga ttctatcaag
     2161 tggtgaagtc gttccattcc gttcgtatca caccggaaat ttattcaagc atcaaaaatg
     2221 ggcaaatgaa ttgcgggcat ttctcaatat ttccgaaggg ttcgacgaaa ctccgcaagg
     2281 ctttttcaag gcagcacaac aggctggagc catgatcgca cagcgtcaac aagaggcgct
     2341 cacaggctcc aatgccgcca tgcgcaacac agacggtgtc aactggcagt tacagtcctt
     2401 tggcataggg gcaacgcccg gcacacgttg gtactcgcct gacttcaaaa ctcaaggtag
     2461 ttttctcttt ttagcacaaa aaattgccgg gcaggcaagt acgggcttcc ttgcctctct
     2521 cggaaaaacc cttttcaaac agtccatttc gctttatgga ttcgacgtct cagacacacc
     2581 aaacatcgaa aaagcggata cctacgcctc tctctccgcc gcgctggata ctcacttctc
     2641 agcctttacc agcaaccaaa ctgaagcgca acaaattctc aacccgtgga cccagcaacc
     2701 gttggcagac tggggcgtga aatatccgtt acagcaattc caatccaatg caagattcag
     2761 tcaactcgta gtgatgttca accccaaagg catctacatc gctacactag gaaccttccc
     2821 agaagacaag cttgaagaaa ttacccgtct tggcgtggaa ttggtcaagg cacatgggag
     2881 ttaaaaatag gacaatcgtc ctccgaattt cattacaatg atacgaagtc tccagagaga
     2941 ccccggaata taatttgaat ctaaatcagt tgtctttctg gagaaaaaaa tgtacacacc
     3001 caaaccccta aaatgggatg atgcactcag tactggcaac caaaccctcg acgagcaaca
     3061 caagtacctg atcgataccc taaatagcct tggcgatgca attattgagg ggcacggaat
     3121 tgacgccatt gccagaattc taggcaggtt gcgcttctat gctggctggc attttgggcg
     3181 cgaagaagaa tgctttgaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 aggttgcgct tctatgctgg ctggcatttt gggcgcgaag aagaatgctt tgaaagctat
     3361 cactgcccgg cagccgaaaa gaacaaaaaa gcacatgccg tgtttatcga aaaattcgat
     3421 aaatatcaag acaaatttca cgaaagtggt ggctcgcttg atttagcgct aaaagtccat
     3481 gaagaaattt cagactggat cgtaaatcac atcatgatcg tggacggcga gttatatcct
     3541 tgcattcata atcaccccaa gccgctcaaa aaataatata aaagcgccgc agaattctgc
     3601 ggcgcttttc gttttaatca agcaccatat ttggctcgat acaaaagaat tgaataaatg
     3661 atggcagtca gcaccccaat aattgataag ataattccaa ccactgccaa ccgtttattt
     3721 tcagaccgcc tgcctaataa accaaagaca ataccggcaa ttcccaacgc cgtccctgcc
     3781 atgggaatta atccggcaca taaactcaat accccaaaac ctgccgaaag cagggcatag
     3841 aacttattga gtcgctcttc cgggtcaaga gaagcagggt ctgtttgcat ggctattctt
     3901 cttcaccaga tagtaatttg attcgttccc aaggcttcag gctttcatcc tctgccaata
     3961 agcttttcaa ctcatacagc tcatcgcgca gagccgccgc cttctcaaac tcaagatttt
     4021 tcgcagcatc tttcatctgc ttttccatct catgcattac ctggcgcaac tcgttgcgcg
     4081 gcatttcatt cttattctta accttatact cgcccttcat ttcgcccacc gccttggcag
     4141 acatttcctc ggtcagatca tgaattgcct tggtaatact aaacggcaca ataccctttt
     4201 cttcgttata tttcacctgc ttggcacggc gacgattggt ctcgtcaatg gcgcgtttca
     4261 tcgaatcggt cattcggtca gcgtacatga tgacacgtcc attcacatta cgcgccgcac
     4321 gcccaatcgt ttggatcaat gcagtatcag agcgcaagaa accttctttg tccgcatcga
     4381 gaattgccac cagcgacact tcaggcaagt ccaacccttc gcgcaaaagg ttgataccca
     4441 ccaacacatc aaaattacca aggcgtaaat ctcgcaagat ccctacgcgc tccagcgttt
     4501 ccacttcaga gtgcaaataa ttaaccttga tgcccaactc tttcatgtaa gtagttaggt
     4561 cttctgccat gcgtttggta agggtagtga cgaggatacg ctcgcctctt tctacccgct
     4621 gcttgatctc tccgaccaag tcatctacct gccccttgac cgggcgcaca atcacttcag
     4681 ggtctatcaa ccctgttggg cggataatct gctcaacgac ttgctcggca tgcttcattt
     4741 cataatcagc tggcgtcgca gatgtgtaaa tcgtggagcc catcacctgc tcaaattcat
     4801 cgaacttgag cggacgatta tccaatgcag atggtaaacg gaaaccatat tccaccaatg
     4861 tctctttacg agcgcggtca ccgttataca tgccgcgtat ttgcggcaca gtcatatggc
     4921 tctcatctag gatcaacaaa taatcgctgg gcaaaaagtc aatcatcgtc catggatgtg
     4981 aaccggcagg gcgtccatcc aaatggcgag aataattctc aatccctgaa caatagccga
     5041 cttctttgag catttccaag tcatatcgag tccgttgctc aatgcgttgt gcttcaagaa
     5101 actttccttc gcttttatac aaagccaagc gttcttccaa ctcagtttca atattcttaa
     5161 tcgactcttt caggcgttct tgatcggtga gaaattgctt tgccgggtaa atcgaaattt
     5221 cttgcggttc ttcataaatt tcaccggtca acgggctaaa ttgcataata cgttctactt
     5281 catccccaaa gaaagtgatt ttgtaacctt ttttatcttc atatgcagga atgatctcca
     5341 acgtatcacc acggacacgg aaagtaccag ggcgcaattc catgtca
//
LOCUS       DAOQFT010000095         5381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7769_length_5381_cov_7.350732, whole genome shotgun sequence.
ACCESSION   DAOQFT010000095 DAOQFT010000000
VERSION     DAOQFT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7769_length_5381_cov_7.350732"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLR93_03690"
     CDS             complement(<1..709)
                     /locus_tag="PLR93_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH26093.1"
                     /translation="MTESALLNDRYQLDELLGSGGMANVYRARDVVLDRPVAIKVLRK
                     EYSTNENFQKQFWLEARSAANLSHPNIVTVHDFGAADDLLYIVMELVPGKDLKQLVRE
                     RGRFPYQQGIPLVIQACAGVGYAHRAGLVHCDIKPHNMLVSKDMRLKVTDFGIARALA
                     TIKPGERNDVVWGSPLYFAPEQAAGEAPTPASDVYSLGVVLYEILTGTTPFTASTADE
                     LARLHISARPLTISEYVP"
     gene            882..1325
                     /locus_tag="PLR93_03695"
     CDS             882..1325
                     /locus_tag="PLR93_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26094.1"
                     /translation="MLGEPILVMLVEDNADHAELVIRTMESHHIPTRIQHVTDGESAL
                     DYLLRRNNFSDPETSPRPHMILLDLRLPRVDGLEVLRIIKEEEDLKSIPVVILTTSEA
                     EKDVMHAYEHYVNSYLVKPVGFEDFSSLMKDLGIYWLGWNKQLRA"
     gene            1332..2714
                     /locus_tag="PLR93_03700"
     CDS             1332..2714
                     /locus_tag="PLR93_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH26095.1"
                     /translation="MSNDIIHILLVEDEDPHAELIQRAFEDQGSEFQIHRVRSLTEAR
                     EHRRLNEPKLIIADWRLPDGESLELLPNHRDPLATPIILMTSYGNERIAVEALKSGAL
                     DYVVKSPESMLDMPHLARKALDQWKSRADRIHMQEALTESEAHFRLLAENASDMITRI
                     NTNGQMFYISPACKTILGYQPEEMLGVKVFDLINPEDQPQIRELFSQKPEDSPYTITY
                     RAKHKSGHYVWLETSARAIIDHSTDEVIEIQAASRDITERKRAEEALQLAHNQLRDAY
                     ERTIEGWVRALDLRDRETEGHTQRVTDITIRVAQQLGFSEEELSHIKRGALLHDMGKI
                     AIPDEILQKPGPLNETEWERMRQHPMYAYEMLSPIAYLNPALDIPFYHHERWNGSGYP
                     HGLKGEKIPLSARLFAIVDVWDALRSDRPYRKAIPREQVIEYLSENANILFDPKLVEV
                     FLKFVKENIP"
     gene            2756..2854
                     /gene="ffs"
                     /locus_tag="PLR93_03705"
     ncRNA           2756..2854
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLR93_03705"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2900..3256
                     /locus_tag="PLR93_03710"
     CDS             2900..3256
                     /locus_tag="PLR93_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26096.1"
                     /translation="MSSSKIITILIALAIGTGLGLVYGWVLSPVEYTDVTPDVLREDY
                     RVDYVLMTAEAYQTDFDADAAARRIAQLGSQAPAEIVSSALTYAQKNGFTQDEQNALQ
                     TLLTAMQTYQPDGSQP"
     gene            3253..4149
                     /locus_tag="PLR93_03715"
     CDS             3253..4149
                     /locus_tag="PLR93_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26097.1"
                     /translation="MKQFLQNSILHIVLAVLIGFGLGLAYSWLLAPVTYVDAHPALLR
                     ADFKDQYRIIIAASYASNKDLPRAKARLDLLGDVDPVSELSAQAQRMLAAGESFDEAR
                     PLAQLATDLQQGYVSEPFTPTPFPTFNTPNAQFTSTPTESGDQPEVSEEATFTPAAPT
                     LLFEQTPITPIVQSTLTQRPTFTPTAGPGAPFALVNQETVCDPGMSRVMQFMLLNSKR
                     EQVPGAEIIVTWSQGEDRFFTGFKPELGNGYADFLMESNTTYNIRVVTGGAFVADVRA
                     PDCTDPNGATYSGGLVLTFQQP"
     gene            4213..4734
                     /locus_tag="PLR93_03720"
     CDS             4213..4734
                     /locus_tag="PLR93_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26098.1"
                     /translation="MNTSLPEQKPGLVNAIAWMTLASGIINLFWGFVASATALGTIVG
                     VICLPITILPTILGIFEIIYAAKLLSAQPQPVQPSNSIAVFEILTFLMGNVFSMVVGI
                     LSLIFYNDLTVKAYFARINAGNAVAPEPVIVSTPAQIEEPQPEILPEPIVEIIPEEPI
                     QTAKPKKPRKTSK"
     gene            4997..>5381
                     /locus_tag="PLR93_03725"
     CDS             4997..>5381
                     /locus_tag="PLR93_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26099.1"
                     /translation="MKSHRFFFGFMALLVAVSLACGGSGSPTANPTLPPVPTNPPAQQ
                     QDQGQQQQDQGQVPSDGTILTYTDQNNLWAFDLPGDWQHEYKDLGDTLYTDATAYLDT
                     FTSPDGSAYIDSLVMFANEGVTVNNS"
BASE COUNT         1431 a         1378 c         1228 g         1344 t
ORIGIN      
        1 ctggcacgta ttcgctaata gtcagcggac gtgcggagat gtgcaaacgt gccagttcat
       61 cggcagtgga ggcggtgaaa ggggtggttc ctgtcagaat ttcatagagc acaacgccta
      121 acgagtacac gtctgaagct ggggttggcg cctcgcctgc ggcttgctcg ggcgcaaagt
      181 agagcggaga gccccatacg acatcgtttc gttcaccagg tttgatggtt gccaacgcac
      241 gtgcgatacc aaaatctgtg actttgaggc gcatatcttt ggaaacaagc atattatgcg
      301 gcttgatatc gcagtgaacc aagccagcgc ggtgcgcata ccctacccca gcacaagctt
      361 ggataacgag cggaatcccc tgttgataag ggaaacgtcc gcgctctcgt acgagttgct
      421 tgaggtcttt gccaggaaca agttccatga cgatgtagag caaatcatcg gcagcgccaa
      481 aatcatgaac ggtgacaata ttggggtgcg aaagattcgc cgcggaacgc gcttccagcc
      541 agaattgttt ttgaaaattc tcgttggtgg aatattcttt gcgtaagacc ttaatcgcaa
      601 caggacggtc tagcaccaca tcgcgcgcgc ggtacacgtt cgccatgccg ccagagccga
      661 gaagttcgtc aagttgatat cggtcgttta gtaatgcact ttcggtcatt gtggtgagat
      721 tataccgtga gttctacata gaggcacgga gatagaaaat cgatattttg gggagtttat
      781 atgtttagtt gtcttaacag gtctgttaaa taaagttgtc ataacgattt tcacccgtta
      841 ttcgtaaatc gtttacaata tgactgttta ggagatccac catgctaggc gaaccaatcc
      901 ttgtcatgct cgttgaagat aatgctgacc atgcagaact tgtcatccga accatggaga
      961 gtcatcacat tcctacacgg attcaacatg taaccgatgg cgaatcagca ttggattacc
     1021 tcctgcggcg caacaacttt agtgaccccg aaaccagccc tcgcccacat atgatccttt
     1081 tagacttacg cttaccccgt gttgatgggt tggaagtact gcgaatcatc aaagaagaag
     1141 aagaccttaa gagcattcct gttgttattc tgacaacgtc tgaagctgaa aaagacgtta
     1201 tgcacgcgta tgaacattat gtcaacagct atctagtaaa gccggttggg tttgaagatt
     1261 tcagcagctt aatgaaagac cttggcattt actggttggg ttggaataaa caactacgcg
     1321 cttaatccct catgtcgaat gacatcattc atattctttt agttgaagac gaagatccgc
     1381 atgcggagct gattcagcgc gcttttgaag accaggggtc tgagtttcag attcaccgtg
     1441 tgcgttctct tacagaagcc cgcgaacata gacgcttgaa cgaacccaaa cttatcatcg
     1501 cagattggcg gctcccagat ggggaaagct tggaattgct ccccaatcac cgcgacccgc
     1561 tggctactcc catcattcta atgacaagtt atggcaatga acgcatcgcg gtagaggcgt
     1621 taaagtctgg tgcgttagat tatgtggtga agtcccctga atcaatgctg gatatgcctc
     1681 acttggcacg aaaagcactg gaccaatgga agtctcgggc agaccgcatt cacatgcagg
     1741 aagcccttac agagagtgaa gctcactttc gcttgctggc tgagaacgcc agtgacatga
     1801 tcactcgcat caatacaaac gggcaaatgt tttatatttc accggcttgt aaaaccattc
     1861 ttggatatca gcctgaagaa atgttagggg tcaaagtatt cgaccttatc aaccccgaag
     1921 atcaacctca gatcagagag cttttttctc aaaaacccga agattcccca tacactatca
     1981 cctatcgtgc caaacacaaa agcggacatt acgtttggtt ggaaacctcc gcacgtgcca
     2041 tcatcgatca cagcacagat gaagtcattg aaattcaagc tgcatcacgt gacattacag
     2101 aacgcaagcg agcagaagaa gcactgcaac tggcacacaa tcaacttcga gatgcctacg
     2161 aacgcaccat tgaaggttgg gtccgcgcat tagacttgcg tgaccgcgaa acagaaggtc
     2221 acacgcagcg cgttaccgat attacgattc gggttgcaca acagcttggg ttcagcgaag
     2281 aagaactctc ccatattaaa cgaggcgcat tgctccacga tatgggaaag atcgccatcc
     2341 ccgatgaaat tcttcaaaag cctggtccgc tcaatgagac ggaatgggaa cgcatgcggc
     2401 agcaccccat gtatgcgtat gagatgctct ctcccattgc atacttaaac cccgccttag
     2461 atattccctt ctatcatcac gaacgctgga acggaagcgg ttatcctcat ggattaaaag
     2521 gtgaaaaaat tccgctatcc gcccgtctct tcgccattgt agatgtatgg gatgcgctcc
     2581 gcagtgaccg cccgtatcgc aaagcgatcc cccgcgaaca agtcattgaa tatctcagtg
     2641 agaacgccaa tattctgttc gaccccaaac tagtagaagt tttcttgaaa ttcgtaaaag
     2701 aaaacattcc gtgatttgac acccccctca tcatcgggta tccttgcgcc tgtccgggca
     2761 ggcccggcgc ggcgaagcct ttcgaacccc gccaggatcg aaagatagca acggtaagga
     2821 agagtcttcg ggtgccgccg gtcgcctgct cggatatttt tatagtgggt caaaattctc
     2881 aaagcagtat aatttccaca tgtcttcatc taaaattata acgatcctca ttgcacttgc
     2941 cataggcact gggcttggac ttgtgtatgg ctgggtgctc agcccagtgg aatatacaga
     3001 tgtaacgcca gatgttctgc gcgaagatta ccgtgtagat tatgtgctca tgaccgccga
     3061 agcttatcaa accgattttg atgcagatgc tgcagcacgc cgcattgctc aacttggcag
     3121 ccaagctcct gctgagatcg tttcttctgc attaacctat gctcaaaaga acggctttac
     3181 gcaagatgaa caaaatgctt tgcaaaccct tctaactgcc atgcagacgt atcaaccgga
     3241 tggaagccaa ccatgaagca atttctgcaa aattccattc tgcacattgt gctcgcggtt
     3301 cttatcgggt ttgggcttgg tctggcgtac tcgtggcttc tcgcccctgt cacctacgtg
     3361 gatgcgcatc cggcgttatt gcgcgcagat ttcaaagatc aataccgcat catcatcgcc
     3421 gcatcctatg cttcgaacaa agacttgcca cgcgccaaag cccggctgga cttactcgga
     3481 gatgtagacc cggtcagtga attgagtgcc caagctcagc gtatgctggc agcaggcgaa
     3541 tcatttgatg aggcacgtcc gcttgcgcaa ttagcaacag atcttcaaca agggtatgtg
     3601 agcgagccgt ttacgcctac acctttcccc acttttaaca caccgaacgc ccagttcaca
     3661 tccacgccca ctgaaagcgg agaccaaccc gaggtcagtg aagaggcgac gtttacacca
     3721 gcagcgccta cccttctctt tgaacagact cctatcacgc ctattgttca atccacattg
     3781 actcagcgcc ctaccttcac accaacagcc ggacccggcg ctccgtttgc attggtcaat
     3841 caagaaacag tttgcgaccc ggggatgtct agagtgatgc aattcatgct cttgaattca
     3901 aaacgagagc aagtgcctgg cgccgaaatt attgtgacgt ggagccaggg cgaagaccgt
     3961 ttctttactg gcttcaaacc cgagcttgga aatgggtatg cagatttctt aatggaatct
     4021 aatactacgt acaacattcg ggttgtaacc ggtggcgcat ttgtggcaga tgtgcgcgca
     4081 ccagattgca cagaccccaa tggtgcaacc tattcgggcg ggcttgtgct tacattccag
     4141 caaccttaat gatttgcaac caatcgtgtg ttcaatcgtt atattcaaca agcaaatctt
     4201 aaggagcaat acatgaatac ctctcttcct gaacaaaagc ccgggcttgt taatgccatt
     4261 gcatggatga cgcttgccag tggcatcatc aatctttttt gggggtttgt tgcatctgca
     4321 actgcacttg gcacaatcgt tggtgtcatt tgtttaccga tcactatcct tcctacgatc
     4381 ttaggaattt tcgagatcat ttacgctgcc aaattattaa gtgctcaacc ccagcctgtt
     4441 caaccttcaa actccattgc cgtttttgag attttaacgt tcttaatggg aaatgttttc
     4501 tcaatggtgg tgggtattct ctctctgatc ttctacaacg atctcacggt taaggcttac
     4561 tttgctcgta tcaacgcagg aaatgctgtg gcgccagagc cggtcattgt ttcaacacct
     4621 gctcaaattg aagaaccgca gcccgaaatt ctccctgaac caattgtaga aatcattcct
     4681 gaagagccaa ttcaaactgc aaagcccaaa aagccacgaa aaacgtcaaa ataaatttat
     4741 cttgaaatta gattcacagc atggaacttt ttcacagcct ttgtcgttta taggcagtca
     4801 aaaaaatcaa gttccatgct gtgatttctt ctagcatcgg attccctgct ttttcgtagc
     4861 atttctaatc cgaattccag atgtatgcaa cgaatggcag ggcgtgtaaa atgggactaa
     4921 ggtacccctc aaaaaaataa ggtcataggg tatccttgcg tccatcatca ataaagatag
     4981 aggagaatac aaaattatga aatcacatcg tttctttttt ggctttatgg cgttgttagt
     5041 agccgtcagc ctggcatgtg gtggttccgg ttcccctacc gctaacccca ccttgccccc
     5101 cgtccccacc aatccccccg cccagcagca agatcaaggt cagcaacaac aggatcaggg
     5161 acaagtgccc agcgacggca ccatcctcac ctatactgac cagaacaatc tgtgggcatt
     5221 tgacctcccc ggtgactggc agcatgaata caaagacctt ggcgacaccc tttacaccga
     5281 tgcaaccgcc tacttggata ccttcacctc ccccgatggt tccgcataca tcgacagcct
     5341 cgtcatgttc gcaaacgaag gtgtgaccgt gaacaactcc a
//
LOCUS       DAOQFT010000096         5338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7866_length_5338_cov_6.070982, whole genome shotgun sequence.
ACCESSION   DAOQFT010000096 DAOQFT010000000
VERSION     DAOQFT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7866_length_5338_cov_6.070982"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2125)
                     /locus_tag="PLR93_03730"
     CDS             complement(<1..2125)
                     /locus_tag="PLR93_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH26100.1"
                     /translation="MSTTVMQPGLPGKSRSKVVTKPATDFYGGIGWVIGLLLTLFVSA
                     PVIWFLTQNASRADRADILAYGLAAAWWLNPLSLGGLFYVFSNIRMWRFWTLPALFVW
                     LLINAMVSASIASDAAREWVLNALFIGGLVILGLLFLYIQLRVFVWAASGNASRFFFG
                     IIGWALLNGLVVWALVNPDVLNIVLVALGFIFRVVFAIGFAIIQFVAIFWFMSQTRVE
                     IIRPGDPKQITFDDYKGQPNLLKMVRQWIYLLSDREQFQKMGGQFITGLLLYGEPGTG
                     KTLLAKAMAGEAGIAFISIEGSGFRGMFWGVDVLRMVQFVGRARKLAREYGACIAYID
                     EIDAVGMSRGSVMGGGQAMGGMMGMGAGSGALTRLLYEMDGIGTLTPWEKLRARVYQM
                     LRRKAPVRNWHVMFMGSTNRPDVLDPALVRPGRFDRKIAVGLPDRGGRREIVKYYLSK
                     IKHDETVDIEALVSDTSWATPARIMSAVTKDAVRLALFDGRDKISQRDIELAFQEQAI
                     GLENPIEEMEEDQRRQVAYHEAGHAVAQYHLRPDERIVRVSIIRRADALGYVLPVSNF
                     DVYAMPLRRFVADIMVSMAGHVATKVFFGEYWTGATGDFQNVRARLWQLAHYGYFGPP
                     ITMDSAMGAEGGYNGRGKIVEKFWQQLEDQTEQLLRTHRIEVEAVAQALLERGDLTGR
                     ECVEIIRQASGEGQADKTETDKMLSA"
     gene            2298..4094
                     /locus_tag="PLR93_03735"
     CDS             2298..4094
                     /locus_tag="PLR93_03735"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNH26101.1"
                     /translation="MQPTPLLYPWLLSVTLTFLTGLYAFRYRRHVAAIPFILMCFFAS
                     FWALCYIFELGATSLELKTWGVKLGYIGILGLPLSWTAFALAYSGHTSWITKRNIMLA
                     LIFPIITLIVIFTNQYHHWFFTDFGLQTDSASGLILIYDPLGWWFWLHAVYTYGILIF
                     GSYFLVREYWEKREVYRSQIIINMTAILLPWISNGIVLAGLLPVRIDITSVMFSISIL
                     IFGWGFLRYQLLDILPVAHRAVFESISDSVIVLDPELRIIELNPAAVEMFKLNIGEVL
                     GKSFQSVFRPWVQLNDKALQTHGYHREIVLENDGEPVRWLHLYVSALRNGASTTEGHI
                     ITLRDVTSIKENESALAIARDEAMQANSFKSQLLANVSHELRTPLGIILGYTDLIVRR
                     SYGDLTEKQVNILGRIKDSTQYLDGLVSELLDQAQLDSGKLQLSTRPFEPREVLGSVC
                     NQLSILAEAKNLEFHYVISENMPISIVGDSQRLKQILVNLISNAIKFTEVGRITVNIA
                     VLPSNTEWIMQVIDTGPGIPEESLGTVFEPFKQLANAHKSLRKGYGLGLSIARQLIRL
                     MGGNISLESALGKGTTFTITLPLVVETEDSDE"
     gene            4087..4470
                     /locus_tag="PLR93_03740"
     CDS             4087..4470
                     /locus_tag="PLR93_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26102.1"
                     /translation="MNSFPNAIVVEDDPYLLDIYADTLRGMNIQVETYQDGMSALKGL
                     KNATPNLIILDLNLPKLSGIEIFHALRNQPQTAETWVLIITANPAQAAELSKNEIESQ
                     NLLILTKPISVDQLDQLAQRIIFHS"
     gene            4500..>5338
                     /locus_tag="PLR93_03745"
     CDS             4500..>5338
                     /locus_tag="PLR93_03745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH26103.1"
                     /translation="MDSTFATLQPLLDQAISLRLTASSRTLNSSSHEEAFRLFNGFYE
                     GFPDLVLDVYGETLVIHDYSEAPNSVFIQELTSYIRTSLKWLRAGILKSRHGTSQKEK
                     RGVLIFGTEPNRKIKEHGIWYSIDLTMNRDASFYLDTVNLRKWLIENSRGKSVLNTFA
                     YTGSLGVAALAGEAAKVIQTDLNRQFLNLAKESCTLNGFPVEKPNFIAQDFFSVIARF
                     KAGKQAFDCVIIDPPFFSSTSRGKVDMEHESERLINKVRPLIQDGGTLIAINNAVFLS
                     GKE"
BASE COUNT         1447 a         1414 c         1162 g         1315 t
ORIGIN      
        1 gtgcactgag cattttatct gtctcagtct tatcagcctg accttcacca gaagcctgac
       61 ggataatttc aacacattcg cgcccggtca ggtcaccgcg ctcgagcaaa gcttgggcaa
      121 cggcttctac ttcaatgcga tgggtgcgca agagttgctc ggtctggtct tccaattgct
      181 gccagaactt ctccacaatc ttgccacgcc cgttataacc gccctctgcg cccatggcac
      241 tatccatggt aattggcgga ccaaaataac cataatgtgc caactgccac aaccttgcgc
      301 ggacgttttg gaagtcgcct gtggcaccag tccagtattc gccaaagaag actttcgtag
      361 ctacgtgccc agccatagag accataatat cggctacaaa gcgtcgcagc ggcatggcat
      421 acacgtcaaa atttgagacg ggcaacacat accctaatgc atcggcacgg cggataatac
      481 ttacgcgaac aatgcgttca tcggggcgta agtgatattg cgctacagca tgcccagctt
      541 catggtatgc cacttgccta cgctggtctt cttccatttc ttcaatgggg ttttcaagac
      601 caatcgcttg ttcctggaag gctaattcaa tatcacgttg agagatttta tcgcgcccat
      661 caaacaatgc caaacggact gcatcctttg tcactgcaga catgatacga gcgggagttg
      721 cccaagacgt gtcactgacc aacgcttcaa tgtctacggt ttcatcgtgt ttgatcttgc
      781 tcaagtagta cttcacgatc tcacggcgac caccacggtc tggcagacct acagcaatct
      841 tgcggtcaaa acgaccgggg cgcaccaatg ccgggtcaag cacatcgggg cggttggtag
      901 agcccatgaa catcacatgc caattgcgga caggtgcttt acggcgcaac atttgataga
      961 cacgcgcacg cagtttttcc cacggcgtca acgtgccaat cccgtccatt tcgtagagga
     1021 ggcgggtcaa cgcgccagag ccggcgccca tacccatcat gccacccatc gcctgcccgc
     1081 cacccatcac acttccacga gacataccca cggcatcaat ttcatcgata taggcgatac
     1141 aagcgccata ttcacgtgca agcttacgag cacgcccaac gaattgaacc atgcggagga
     1201 cgtccacacc ccagaacatg ccacggaaac cagagccttc aatggagata aaagcgatac
     1261 ccgcttcccc tgccatggct ttggctagca aggttttacc cgtaccaggt tcaccataga
     1321 gcaacaagcc ggtgatgaat tgtccgccca ttttttggaa ttgttcacgg tcagacaata
     1381 gataaatcca ctggcggacc atcttaagca ggttcggctg acctttgtaa tcatcaaagg
     1441 taatctgctt cgggtcgccg gggcgaatga tctccacacg tgtttgagac atgaaccaga
     1501 agatcgccac aaattggatg atggcgaagc caatggcaaa tacaacgcgg aaaataaagc
     1561 ccaaggcaac aagaacaata ttcagcacat cagggttaac caatgcccaa acaaccaagc
     1621 cattaagtaa agcccaacca ataatgccga agaaaaagcg gctggcattg ccagatgccg
     1681 cccaaacaaa tacgcgtaat tgaatgtata aaaacagcaa acctagaatg actaaaccgc
     1741 caatgaaaag ggcgttcaag acccattcac gcgccgcatc agaagcaatg gacgcagaca
     1801 ccatggcatt gatcaatagc caaacgaaaa gagccgggag cgtccaaaag cgccacatac
     1861 ggatgttgct aaaaacatag aacaacccgc cgagcgatag cggattaagc caccaggcgg
     1921 ctgccaagcc ataggcgagg atatctgccc ggtcggcgcg cgaggcgttc tgcgtcaaaa
     1981 accaaatgac tggagctgaa acgaaaagag tgagcaaaag cccaatgacc catccaattc
     2041 ccccataaaa atcagtggca ggcttggtta ccactttgga acgtgactta ccgggtaagc
     2101 ccggttgcat aacggttgtg gacatgagac ctctgagcga aagaagattg gggcaagttt
     2161 atcttgaaaa cgtgataaag aaatgagaag agctggctat tagttcttgg atgcacaaaa
     2221 agggtgattc ttacgctttc gcctaattat gttcccgcaa ttcttttacc ccggtatcaa
     2281 gttgtataat tgttcaaatg cagcccacac ctctacttta tccttggcta ctgtctgtaa
     2341 cgcttacttt cctaacagga ctatatgctt ttcgttaccg caggcatgta gctgctattc
     2401 cgttcatttt gatgtgcttc ttcgcatcat tttgggcgct ttgttatatc tttgaattag
     2461 gcgctacaag cttggaatta aaaacatggg gtgtgaaatt ggggtatatc ggcattcttg
     2521 gtcttccact ttcatggacg gcttttgcac tggcgtattc tggtcacaca agctggatca
     2581 ctaaacgcaa catcatgctg gcgttgattt ttcccattat cacgctcatt gtgatcttta
     2641 ccaatcaata ccatcactgg ttcttcacag attttgggtt acaaaccgat agcgcttctg
     2701 gattaattct catctatgat cctttaggtt ggtggttttg gttgcatgcc gtttacacct
     2761 atggtatttt gatcttcggc tcttatttcc ttgttcgcga atattgggaa aaacgcgaag
     2821 tgtatcgctc tcagatcatt atcaacatga ctgcaattct tctgccgtgg atatcaaatg
     2881 gtattgtgct tgctggacta cttccagttc gcatcgacat tacctcggtc atgttctcca
     2941 tctcaatctt gatcttcggg tggggctttc tgcgctatca actgctggat attctgcctg
     3001 ttgcccaccg cgccgtcttt gaaagtatct ccgactcggt cattgtcctc gacccagaat
     3061 tacgcattat tgagcttaat cccgccgccg tggagatgtt caaattaaac attggcgagg
     3121 tgcttgggaa atcttttcaa tcggtcttca gaccttgggt gcaattaaat gataaagccc
     3181 tgcaaaccca tgggtatcat cgcgagatcg tattggaaaa tgatggcgag cctgtacgtt
     3241 ggttacacct ttatgtaagc gctttgcgca atggtgcatc gaccactgaa gggcatatca
     3301 tcaccttacg tgatgtcacc agtatcaaag aaaatgaatc tgcgttagcc attgcccgtg
     3361 atgaagccat gcaggcaaat agcttcaaat ctcaattact tgccaacgta agccacgaac
     3421 tgcggactcc gctcggtatc atcctcgggt acacagacct gatcgtacgt agatcctacg
     3481 gcgatttgac cgaaaaacaa gtcaacattc tggggcgcat taaagacagc acccaatatt
     3541 tagatggact ggtttctgaa cttctcgacc aagcccaact tgacagcgga aaattacagc
     3601 tctccacacg tccgtttgag ccgcgtgaag tgcttggatc ggtttgcaat caactaagca
     3661 ttctggctga ggcgaaaaac cttgagtttc attatgtcat ctcagagaac atgcccattt
     3721 ccattgtcgg agatagtcaa cggctaaagc aaatccttgt caatctcatc agcaatgcaa
     3781 tcaagttcac agaagtcggc aggattacgg tcaacatcgc agtattgccc tccaacacag
     3841 aatggatcat gcaagtcata gacacaggcc caggcatccc agaagagtcg cttggcactg
     3901 ttttcgagcc attcaagcaa ttagcaaatg cccataaatc cctgcgcaag ggttatggct
     3961 tggggctttc aatagccagg caacttatcc gactcatggg cgggaatatc tcgcttgaaa
     4021 gcgcccttgg caaaggcact accttcacca tcaccctccc tcttgtagtt gaaacagaag
     4081 atagcgatga atagttttcc caatgcaatc gtcgtagaag atgacccata cctcttggat
     4141 atctatgccg atactttacg cggcatgaac attcaggttg agacctatca agatggcatg
     4201 agcgccttga aaggactcaa aaatgctacg cccaacctca tcattctcga tctcaatctt
     4261 ccgaaacttt ctggcatcga aatctttcat gcgcttcgca accaacccca aaccgctgaa
     4321 acatgggtct tgatcatcac cgccaacccc gctcaagccg ccgaactttc taagaacgaa
     4381 attgaaagcc aaaatctgct catcctcaca aagccgatca gcgtagacca gctcgaccaa
     4441 cttgctcagc gcattatttt tcacagctaa actcaccaca agccggatac aatcagtcaa
     4501 tggactcaac tttcgccact ctgcaaccat tgctagacca agccatttcc ctgcgactta
     4561 ccgcctcttc ccggaccctg aacagctcca gccacgaaga agcgtttcgc ctcttcaacg
     4621 ggttctacga aggcttccca gaccttgtgt tggatgttta tggtgagacc ctagttattc
     4681 acgattattc agaagctcca aattctgttt ttattcaaga acttacaagc tatattcgca
     4741 cttctctaaa gtggctacgc gctggaattc ttaaatcccg ccatggtaca agtcaaaaag
     4801 aaaaacgcgg tgtgctcatc tttggcactg agccgaaccg caagatcaaa gaacacggca
     4861 tctggtactc cattgactta accatgaacc gcgatgcaag cttctatctt gataccgtca
     4921 acttacggaa atggctgatt gaaaactcac gcgggaaatc tgtattaaac acctttgctt
     4981 acacaggcag tctaggggtt gctgctctcg caggcgaagc tgcaaaagtg atccagacgg
     5041 atcttaatcg tcaattcttg aatcttgcaa aagaatcctg cacattgaac ggctttcctg
     5101 tcgaaaagcc taacttcatt gcacaagact tcttctcggt tattgcccgt ttcaaagctg
     5161 ggaagcaagc cttcgattgc gtcatcattg acccgccttt cttctcttcc acatcacgcg
     5221 gaaaagtgga catggagcat gaaagcgaac ggctcatcaa caaagtccgc ccgttgattc
     5281 aagatggcgg cactctcatt gccataaaca atgcagtttt tctaagcggc aaagaata
//
LOCUS       DAOQFT010000097         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7921_length_5315_cov_8.691255, whole genome shotgun sequence.
ACCESSION   DAOQFT010000097 DAOQFT010000000
VERSION     DAOQFT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7921_length_5315_cov_8.691255"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLR93_03750"
     CDS             <1..492
                     /locus_tag="PLR93_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNH26104.1"
                     /translation="GRERADAVRAIVNAGIPVMGHLGLTPQSVNQLGGFRAQGKTASA
                     AKRLLEDAQILEEAGAFSLVLESVPARLAEYISKHISIPTIGIGAGTGCDGQVLVTHD
                     LLGLFERFTPKFVRQYAKLHETMNKAFTEYLNDVNTKQFPAAEHTVEMTDEEWAAFQK
                     EVK"
     gene            489..1385
                     /locus_tag="PLR93_03755"
     CDS             489..1385
                     /locus_tag="PLR93_03755"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNH26105.1"
                     /translation="MSENILLIGTGALSTLFAARLSEAGHHVSMLGTWKDGIQALNQN
                     GARVSESNGKEHAFQVHATDEPSEVRNVKHAIVLVKSWQTKRVAEKLKGILSPDGIVL
                     TLQNGLGNRETLARDLGAERVALGVTTTGATLLGPGHVKVGGEGILSLEQNQALGPLE
                     AALRSSNFNLQIVQNAQSLVWGKLIINAAINPLTALLQVPNGELLNHPWARKAMNALA
                     REAAEVAQAEQVNLPFNNPIEAVEEVARKTAQNLSSMFQDVRRGAPTEIDAICGAVTK
                     RGEMHGISTPYNRSCWQLVKAM"
     gene            1533..2435
                     /locus_tag="PLR93_03760"
     CDS             1533..2435
                     /locus_tag="PLR93_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH26106.1"
                     /translation="MRKLFLLLLMGAFLSFPISASAQSDVSLSFATVQLWPEYDDPSM
                     LVIVDFQVAAATSLPATLTFRIPAEANLIAVASDTGNGQFLDHPYTGPNPEGEYQTFS
                     MVLEQNTPYRFEYYQPLSYNGDTRIFSYLWDSEYAVENFQYLFLEPLDVTDIALDPKY
                     TAKETFNGLNYYDGAPVQLAAGEQYSMKVSYTKTTDTLVSQAQSVQIAEPVNEDTPGR
                     VSLANSLPYIIGGLGIIMIAGGLMYYFQWGRHSAGGGKHRRRSRTNTESDATSIYCPQ
                     CGARAKPSDRFCRTCGARLRHDEA"
     gene            2490..3950
                     /locus_tag="PLR93_03765"
     CDS             2490..3950
                     /locus_tag="PLR93_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNH26107.1"
                     /translation="MENLSVTVIYFFYGLAFFSMGLLVAMEGGRSTDARLRMALRPLA
                     GFGLVHAAHEWLEMFRAMGHLNAFFTGIYPLLALSILAFSFLSLAAFGAYLVLGSEST
                     WRVSLIIPLGLEAIWVYGLLIFKSKYSLDEIYTVADVWTRYSIAIPAGLLAAAGLVVQ
                     QRAFRRAGLVSFGRDSLWASVAFIWYSLVGQLFTEPSPLPPSTFLNSALFLNLFGFSI
                     QLLRAITAMFASLFVTRFLRAFQVESDSKIAGLQAAQLHESQQREKLRAQLFRSIVGA
                     QESERQRIARDLHDETGQSLTAIGMGLRGLANETDSKKRLVTLTQLQSLTSDSIRELQ
                     RIISDLRPAHLDDLGLSATLRWYASRVHELTSLHVRVDIEGDEPALDDAVKITIFRIM
                     QEALNNVIKHAQATTVNVKIHYLQKEVRIHVFDNGVGFEMKAVQNRIGRVSLGLAGME
                     ERAALLGGGVTVSSRPGYGTEVEAVVPLHLIDQEEK"
     gene            3947..4597
                     /locus_tag="PLR93_03770"
     CDS             3947..4597
                     /locus_tag="PLR93_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26108.1"
                     /translation="MTIRLLLVDDHAVVRTGLKMLLNGQEDLEIVGEAGSAAEALTET
                     ERVQPTVILMDIGLPDKTGIEATREIKAKFANVNIVALTIHEDEEYFFQMLNAGASGY
                     VPKRAAPEELITAIRAAANGEVYLYPSMAKLLVRDYLNIDRLPEEKIDLGGLTDREHE
                     VFTMLAEGASNQEIADALVISPKTVERHRENIMRKLNLHSRSELVRYAIRKGIIKA"
     gene            5002..>5315
                     /locus_tag="PLR93_03775"
     CDS             5002..>5315
                     /locus_tag="PLR93_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26109.1"
                     /translation="MTTNSILVFILGVAVRLGLPMLVTTLVVIGLRRLDVRWQKEAEI
                     ERNLLVRDETPCWKEQGLSMDEIKARATESGKPCWQIHRLSNGYMREACLGCEIFINA
                     PTP"
BASE COUNT         1335 a         1267 c         1365 g         1348 t
ORIGIN      
        1 gggcgtgaac gagctgatgc agtgcgcgcc attgtcaatg caggcattcc tgtgatgggt
       61 cacctcgggc ttacgccgca atcggtgaat caacttggtg ggttccgtgc acagggcaaa
      121 acagcgtctg cggctaaacg tctacttgaa gatgcacaga ttcttgaaga agctggggcg
      181 ttcagcctcg tgctcgagtc tgtgcccgca agattggcgg aatatatttc aaaacacatt
      241 tccattccaa ccattggtat tggcgcaggg acaggctgtg atggacaagt gcttgtcacc
      301 cacgacttac tgggtctatt tgaacgcttt acaccgaaat ttgttcgcca atacgcaaag
      361 ttgcacgaaa caatgaacaa ggctttcacc gaatatctca acgatgtgaa caccaaacaa
      421 tttccggctg ccgaacatac cgttgaaatg acagatgaag aatgggctgc ttttcaaaag
      481 gaagtgaaat gagcgagaat attttactta ttggtacagg cgcgctctct acattgtttg
      541 cagcccgttt gagcgaagct gggcatcatg tatccatgtt gggcacatgg aaagatggca
      601 ttcaagctct taatcaaaac ggcgctcgtg tatcagaatc aaacggaaaa gaacacgcct
      661 tccaagtcca tgctaccgat gaacctagtg aagttcgaaa tgttaaacac gccattgtct
      721 tggtcaaatc ttggcagaca aaacgtgtgg cagaaaaact taaggggatt ctctcacccg
      781 atggaattgt gttgaccctg caaaacgggc ttggaaatcg cgaaacgctc gcacgggacc
      841 ttggggctga gcgcgttgcc ctaggcgtta ccaccactgg agccaccctg cttggacctg
      901 ggcacgtaaa agttggcggt gagggcatcc tctcactgga acagaatcag gctctcggtc
      961 cgcttgaggc ggctcttcgg tcatccaact ttaatttgca gatcgttcaa aatgcgcaat
     1021 ccttggtgtg gggcaaactc atcatcaatg cggctattaa cccgttgact gcgttgttgc
     1081 aagtgccaaa cggtgagttg ttgaatcatc cttgggcacg caaggcgatg aatgctctgg
     1141 cgcgtgaagc cgcggaagtg gcacaagcgg aacaggtgaa tttgccattc aacaacccga
     1201 tcgaagcggt ggaagaagtg gctcgcaaaa cggcgcaaaa tctttcatca atgtttcaag
     1261 atgtacggcg cggtgctccg acagagatcg atgccatttg tggtgcggtg accaaacgcg
     1321 gagaaatgca cggaattagc acgccataca atcgttcttg ttggcaattg gtaaaagcaa
     1381 tgtaattaag tacgggcggc gcatgcgccg cccgtaccga tataaaaagg gtgacattta
     1441 tctatattct gcatgatttc gtttctgccc tcaaaaaatg cgacatggta agatacatat
     1501 agaagtaaaa catttatcgt taggaggcgt tcatgcgcaa actattctta ctccttctta
     1561 tgggagcctt cctctcgttc ccgatttccg catctgcaca aagcgatgtg tcgctttcat
     1621 ttgccactgt gcaattatgg cccgaatatg atgaccccag catgctggtg atcgtagatt
     1681 ttcaagtagc ggcagctaca agtttgcctg ctacactcac cttccgtatt cctgcagaag
     1741 ccaacctcat cgctgtcgct tcagatacag gcaacggaca attcctcgat cacccataca
     1801 caggacccaa ccccgaaggt gaatatcaaa cctttagtat ggttcttgag caaaataccc
     1861 cctatcgttt tgaatactat caaccgcttt catacaacgg ggatactcgc attttctctt
     1921 atctttggga tagcgaatat gcggttgaga attttcaata ccttttcctt gaacctttag
     1981 atgtcaccga tattgcgctc gacccgaagt acactgccaa agagacgttc aacggattga
     2041 actattatga cggtgcgcct gttcaacttg cggcgggtga acaatattcg atgaaagtaa
     2101 gttacaccaa aactacagat actttagtct cgcaggcaca gtcggtgcaa atcgcagaac
     2161 cggtgaacga agatacgccg ggtcgcgttt cacttgccaa tagtttgcct tacatcattg
     2221 gcggacttgg catcattatg atcgcaggcg gcttgatgta ttacttccaa tggggacgcc
     2281 actctgcagg cggtggtaaa catcggcgcc gttcacgaac gaatacagaa agcgatgcca
     2341 ccagcatcta ctgtccacaa tgtggggcac gtgccaagcc tagtgacagg ttctgtagaa
     2401 cctgtggagc aaggctaagg cacgacgaag cataacgagg agagacccta aaggcgaaca
     2461 aagcgccttt ggggtctttt tatgtaagca tggaaaatct gtctgttaca gtcatctatt
     2521 tcttttatgg gcttgccttt ttcagcatgg gcttgttggt tgccatggaa ggagggcgct
     2581 ctacagacgc gcgcttgcgt atggcgttac gtccgctggc tggctttggt cttgtacatg
     2641 ccgcccatga atggctagaa atgttcagag ccatggggca tttgaatgca ttttttacag
     2701 gcatttatcc actgctggca ttatccattt tggctttttc gtttttatca ctagcagcat
     2761 tcggcgctta tctcgtcctt ggcagcgaat cgacttggcg tgtgagtctc atcattccac
     2821 tggggctcga agccatctgg gtttatggct tgcttatttt caaaagtaaa tattcgctag
     2881 acgagattta caccgtcgcc gatgtgtgga cgcgatactc cattgccatt ccagcagggt
     2941 tgctggcagc ggctgggttg gtggtgcaac aacgtgcgtt ccgcagagcc ggtttggtaa
     3001 gcttcgggcg cgatagttta tgggcatcgg tggcgttcat ttggtatagc ttagtaggtc
     3061 aattatttac agagccaagc ccattaccac cttcaacttt cttaaactcg gcactgttct
     3121 taaatctatt cgggttttcc attcagttac tacgcgcaat tactgccatg ttcgcttcct
     3181 tgttcgtaac gcgttttttg cgcgcctttc aagtggaaag cgacagcaag atcgcaggac
     3241 tacaagccgc acaattacac gagtcacaac aacgcgaaaa actgcgcgct caactgtttc
     3301 gcagcatcgt gggtgcccaa gagtctgaac gtcaacgcat tgcgcgcgat ctgcacgatg
     3361 aaaccggtca gtccttaact gccattggca tggggttgcg cggtcttgcg aatgagactg
     3421 actcaaaaaa gcgcctcgtc actctgaccc aactccaatc tcttacctca gactccatac
     3481 gtgaattgca acgcatcatt tcggacctca ggcctgcgca cctcgatgac ctgggccttt
     3541 ctgccacgtt gagatggtac gcttcgcgcg tccacgagtt gacatccctg catgtacgcg
     3601 tggacatcga aggcgatgag cccgctctcg acgacgcagt gaaaatcact atttttcgca
     3661 ttatgcaaga agcgttgaat aacgtcatca aacatgctca agcgacaacg gtaaacgtca
     3721 agatccatta tttgcaaaag gaagttcgca ttcatgtgtt tgataatggc gtggggtttg
     3781 aaatgaaagc cgtacagaac cgcattggac gtgtctcttt aggcttggca ggcatggaag
     3841 aacgtgctgc gttactcggt ggcggtgtga ccgtctcttc acgcccaggc tatgggacgg
     3901 aagttgaggc ggttgttccg ttacatttga tagatcagga ggagaaatga cgatccgatt
     3961 gttattggtg gatgaccatg ccgttgtccg cacagggttg aagatgttgc tcaacgggca
     4021 ggaagacctt gagattgttg gggaagcggg gtctgcggca gaggcattaa ccgaaaccga
     4081 gcgcgttcaa ccgacggtca ttctgatgga tattggtttg ccagataaaa ccggcatcga
     4141 agccactcgt gagatcaagg caaagtttgc gaatgtgaat atcgttgcgc tcaccattca
     4201 cgaggatgaa gaatatttct tccaaatgtt gaatgctggt gcctctggct atgtacccaa
     4261 acgcgccgca ccagaagagc tcatcaccgc cattcgggca gcagcgaacg gggaggtgta
     4321 tctttacccg tctatggcga agctcttggt gcgcgactat ttgaacattg accgtttgcc
     4381 agaagagaaa attgacctag gcgggctgac cgaccgcgag catgaagtct ttacgatgtt
     4441 ggcagaaggg gcgagcaatc aagaaattgc cgatgcgctt gtgatcagcc caaagacggt
     4501 ggagcggcat cgcgagaata tcatgcgcaa actaaatctc cactcccgtt cagagttagt
     4561 gagatatgcc attcggaagg gaatcatcaa ggcatagata acaaaatcca taaatcacgc
     4621 atcccgtaat atgggatgcg tttttattta agttcgcgtg gtgggtgtca agcgagtaag
     4681 tttatccact ttacacacat ggttttttcg atggggcaga taccctacag cttgaataac
     4741 gcttggggtg ttcgccacag gcaaagggtc ccaaaagatg cggagggtac tcatccaaga
     4801 atggggttgg tgacgaattt actttccttt aaggaagcaa tatcctatag gcagatttga
     4861 gaaaggagaa tgtcatgaac ggttctgagt ttttcatcct gtactttatc gcccgcgttg
     4921 ccatcccatt tggagcgctt ctgctgctgg gcgagttgtt acgccgcaaa gaatccaact
     4981 actggctcaa gtgaggtcga catgactacg aattcaattc tcgtttttat cctgggtgtg
     5041 gctgtgcgat tgggtttacc catgttggta accaccttgg tcgtgatcgg tttacgccgt
     5101 ctggatgtgc gctggcagaa ggaagcggag atcgaacgca accttctggt acgcgatgag
     5161 acaccctgct ggaaggaaca aggactttcg atggacgaga tcaaagctcg cgctactgaa
     5221 agcggaaaac cctgctggca gatccaccgt ctttcaaatg gctatatgcg tgaagcctgc
     5281 ctcggttgcg aaatctttat caatgcacct acacc
//
LOCUS       DAOQFT010000098         5302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7943_length_5302_cov_8.194969, whole genome shotgun sequence.
ACCESSION   DAOQFT010000098 DAOQFT010000000
VERSION     DAOQFT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7943_length_5302_cov_8.194969"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLR93_03780"
     CDS             complement(<1..314)
                     /locus_tag="PLR93_03780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNH26110.1"
                     /translation="MDWTMWVEWVIKGFVLCLILLTGFAYLTLYERRALARIQVRVGP
                     NRAGPQGLLQPIADAVKLIFKEELTPGHVYKLVFILAPVLTVVPSLIIAAVIPLGTSF
                     SL"
     gene            complement(317..2791)
                     /gene="nuoG"
                     /locus_tag="PLR93_03785"
     CDS             complement(317..2791)
                     /gene="nuoG"
                     /locus_tag="PLR93_03785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNH26111.1"
                     /translation="MSKQVTLTINGKQVTAPEGLSVADAAKLAGIDIPVFCHHPKLEP
                     VGMCRMCLVEIGRPVRDRATGQFVMENGQPKIQFMPKLETACTNKVEDGMVVLTESAK
                     ATDGQRGTVEFLLTSHPLDCPICDKGGECPLQNLTMAHGPGKSRFNYNEKQHLEKMVP
                     LGELIYLDRERCIQCARCIRFQSDVAGEAVLGFDERGRYTQIISLSEPGFDSVFSGNT
                     TDICPVGALTTVDFRFGARPWELKAESSICTQCPVGCNTTLNTRREAKADGEVVVKRV
                     MPRQNEEVNEIWLCDKGRFAYHYTEGKERLTKPMVRKDEKLARASWDNAIKTAAENFV
                     ANKKDFVVLASGRLSNEDLFNLKSLADTAGGEAILYSDMAGGELTQLVGVGAGTNLGK
                     MGKGDAILVIASDLYEEAPIWWLRVKQAADRGATLIVANPRQTKLDRFAKYTIRYAYG
                     DEIKTIHDFEKKSKISDEFAKAKNAVVFYGSEGLGVAGTSALASACADLLKETNHIGN
                     ANNGLIGVWQRANDQGAFEVGFRPVADLEKALKGKAVYIVGADPVGDDPNLAKALKSA
                     KFVAVQELRETATTEIADVVFPAQAFTERDGTVVSGERRVQRFYAALAPKGDSKPDYA
                     ITSMLAKQMGVILQGTSASVVFDILANSVEAFNELTYEALAQVKEQWPLVGRNDVYYG
                     GTTYENTKGLGVQLVPVAQAGVSVKIQKVRKEAALRPKEKELLAVPANKLYDLGVTVQ
                     TAEFLDQRIGETKVAMHPTTAKKFEVEDGQAVKVSFEGVQAEAVARLDETIATGVVLI
                     PRSMGIAIHEPVVARVQAKTASGKAK"
     gene            complement(2798..4045)
                     /gene="nuoF"
                     /locus_tag="PLR93_03790"
     CDS             complement(2798..4045)
                     /gene="nuoF"
                     /locus_tag="PLR93_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNH26112.1"
                     /translation="MAYVLLRHRDIPDIEKMSVYKKNGGFSALKNAITKMQPNDVIDV
                     VKNSGLRGRGGAGFPTGMKWSFIDKNSWPHYIVANADESEPGTFKDREIMEKNPFQFL
                     EGLALGAYAVGANAAYIYLRGEFWQVAAWLDEKIAEMEEAGYLGENLFGKDYSLKIYT
                     HLGAGAYICGEETALLESLEGKRGQPRVRPPFPPSVGLYGKPTIINNVETFTNVPMIL
                     ANGADWYKSMGTADSAGVKIFSLSGRVRKPGNYELPFGTTFRQLIYEHGMGIQEGRPI
                     KAIMPAGASSSVIVVDEKVLDTPLDYASVRTFGADLGSASVIVLDDTVDMDWVINKTI
                     HFFKHESCGKCTTCREGNHWMRNLTDRIHSGKGSGDDVKLLLNVAKQMQGKCLCALGE
                     FSTMAVVTSIERFPDDFKKAVKA"
     gene            complement(4048..4542)
                     /locus_tag="PLR93_03795"
     CDS             complement(4048..4542)
                     /locus_tag="PLR93_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNH26113.1"
                     /translation="MSLEKKYSKEVKQILAKYPPEHKRSAVMPLLYLAQREEGYVNKE
                     SMKDIASILDITETEVASIIGFYSLYHDKKAGKYRMQVCNDLPCALRGSDEFLNKLCE
                     NLGVKVGETTEDGLITIEAVTCLASCDRAPMFQTQGPDGIKYHDYMTIDRTMELIEAL
                     KGGK"
     gene            complement(4539..>5302)
                     /locus_tag="PLR93_03800"
     CDS             complement(4539..>5302)
                     /locus_tag="PLR93_03800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNH26114.1"
                     /translation="LDIFELVSGQRMMTTFFRPGGLWRDVPVEFEKAVRDFIKIFPKR
                     IDEYEKLLTKNPLFVDRMVDIGYLSKETALSYGVTGPNLRASGVNWDLRKARPYMGYE
                     QYDFDVPVLTEGDTYARYLVRIEEFRQSLRIIEQALDKLPMGPVRSENRKFVPPPRSE
                     IGVSMEALIHHFKLWTEGFHAPDASIYSAVESPRGELGVLLAGDGGPKPRRVHMRTPS
                     FDNLSVIPEIVKGHLVADLVAILASIDIVLGDIDR"
BASE COUNT         1327 a         1423 c         1271 g         1281 t
ORIGIN      
        1 aaaaggctga atgaagtacc cagcggaata actgccgcaa taatgagcga aggtaccacc
       61 gtcaacacag gcgcaaggat aaaaaccaac ttatacacat gccccggcgt caattcttct
      121 ttgaagatca acttcacggc atctgcgatc ggttgcaaca aaccttgcgg acccgcacgg
      181 ttcggaccaa cacgcacctg aatacgagcc agcgcgcggc gttcatacaa cgtcaaatag
      241 gcaaaccccg tcaaaaggat caaacaaagc acgaagcctt tgatgaccca ttcaacccac
      301 attgtccaat ccataattac ttcgccttcc cagatgcagt cttcgcttgc actcgcgcca
      361 ccacaggttc atggatcgca atgcccatgc tgcgtggaat aagcaccaca cccgtcgcaa
      421 tagtttcgtc gagcctggct accgcttctg cctgaacacc ctcaaagcta accttcacag
      481 cctgcccatc ttccacttcg aacttcttcg ctgtggttgg gtgcattgca actttcgtct
      541 cgccaatgcg ctgatcaaga aactctgcgg tttggacagt cacaccgagg tcatacaatt
      601 tattggcagg cacagccagc aattcttttt ctttgggacg aagagccgct tctttgcgga
      661 ccttttgaat tttcactgaa accccagcct gagccaccgg gacgagttga acacccaagc
      721 ccttcgtgtt ctcataagtc gtgccgccgt aatacacatc attacgaccc acgagcggcc
      781 attgttcctt gacctgagcc aaggcttcat aagtcaattc attgaaagct tccacagaat
      841 tcgcaagaat gtcgaacact actgaagcag aagttccctg aagaatcacg cccatctgct
      901 tagcaagcat agaggtgatg gcatagtctg gtttgctatc acctttggga gcaagcgccg
      961 catagaagcg ttgtacacgg cgttcaccag agacaaccgt accatcacgt tctgtaaatg
     1021 cctgcgccgg gaagaccaca tctgcaattt cagttgtagc agtttcacgt aactcctgca
     1081 cggctacgaa tttggcgctc ttcaacgctt tggcaaggtt ggggtcatcc cccaccgggt
     1141 ctgctccaac aatgtaaacg gctttgccct tcaatgcctt ttcaaggtca gccaccggac
     1201 ggaagccaac ttcgaacgca ccctgatcat tggcacgctg ccaaacaccg atcaaaccat
     1261 tgttggcatt gccgatatga ttggtttctt tcaacaggtc tgcgcaagct gaagcgagtg
     1321 cagaggttcc tgccacaccg agaccttcac tgccatagaa gacgaccgca ttctttgcct
     1381 tcgcaaattc gtctgagatc ttgctcttct tttcaaagtc atgaatggtc ttgatttcat
     1441 cgccatacgc atagcggatg gtgtacttag caaaacggtc gagcttggtc tggcgagggt
     1501 tggcaacgat caaagtcgcg ccgcgatccg cagcttgctt cacacgcagc caccaaatgg
     1561 gagcttcttc atataagtca gaagcgatta cgagaatagc atcgcctttg cccattttgc
     1621 cgagattggt gccagcacca acgcccacaa gctgggtcaa ttcgccgccc gccatatcag
     1681 agtacaaaat ggcttcgccg ccagccgtat cggcaagaga cttgaggttg aacaggtctt
     1741 cattcgaaag acgacctgaa gccaacacca caaagtcttt cttattcgca acaaagttct
     1801 ctgctgcggt cttgatggcg ttatcccatg aagcacgggc aagcttttca tctttgcgaa
     1861 ccatcggctt ggtaaggcgt tctttgcctt cggtatagtg atatgcaaag cgtcccttat
     1921 cgcacagcca gatttcgttc acttcttcgt tctggcgcgg catgacacgt ttcaccacca
     1981 cttccccatc agctttggct tcacgccggg tattgagagt ggtattgcat cccactgggc
     2041 attgtgtaca aatagaagac tctgccttaa gttcccacgg gcgagcacca aagcggaagt
     2101 ccactgtggt caacgcaccc acgggacaga tatcggttgt attccctgag aaaaccgagt
     2161 cgaaaccggg ttcagagaga gaaatgattt gagtgtagcg tccgcgttcg tcgaagccaa
     2221 gaaccgcctc gcctgcaaca tcgctttgga agcggataca acgcgcacat tggatgcagc
     2281 gttcgcggtc aagatagatc aactcaccga gcggaaccat cttttcgagg tgctgtttct
     2341 cgttgtagtt gaagcggctc tttcccggac catgtgccat ggtcaaattc tgcagcggac
     2401 attccccacc cttgtcacag atcgggcagt ccaacggatg ggatgtcaac aggaattcaa
     2461 ctgtaccgcg ttgaccatct gttgccttgg cagattctgt aagaaccacc atgccgtctt
     2521 ccactttgtt ggtacaagca gtttcaagct tgggcatgaa ttggatcttg ggttgtccat
     2581 tttccatgac gaactgaccg gtggcacggt ctcgcacagg gcgaccgatt tccaccaggc
     2641 acatacggca catgccaacc ggctccagct tgggatggtg gcagaagacc gggatatcga
     2701 tccctgctaa ttttgccgcg tctgcaacgc taagtccttc tggcgcggta acttgttttc
     2761 cattgatcgt gagagtaact tgtttgctca ttatctgcta cgcctttacc gctttcttga
     2821 aatcgtctgg gaagcgttca atactggtca ccacagccat agtggagaat tcacccaacg
     2881 cgcataaaca tttgccttgc atttgcttgg caacattcaa gagtaattta acatcatcac
     2941 cagatccctt accgctgtga atgcggtccg tcaggttacg catccagtga ttgccttcac
     3001 ggcaggtggt gcactttcca caagattcat gtttgaagaa gtggatggtc ttattgatga
     3061 cccaatccat atcgactgta tcgtcgagca caatgaccga ggcggaacca aggtctgccc
     3121 cgaaggtacg aaccgaggcg tagtccagag gcgtgtcgag gactttttcg tccacaacaa
     3181 ttacagagga agaagcgccc gcaggcataa tcgccttgat ggggcgtcct tcttgaatgc
     3241 ccatgccatg ttcgtagatc aactggcgga aggtggtgcc gaagggcaat tcatagttgc
     3301 cgggcttacg cacacgacct gaaagtgaaa agatcttcac accggcgcta tcggcagtgc
     3361 ccatgctctt gtaccagtct gcgccgttgg cgagaatcat tgggacattg gtgaaagtct
     3421 caacgttatt gataatggtt ggcttgccat acagacccac agaaggaggg aatggcggac
     3481 gcacacgggg ttgtccgcgt ttgccttcaa gggattcgag caaagcagtc tcttcgccac
     3541 agatgtaagc accagcgccc aagtgggtat aaatttttag agaataatct ttgccgaaga
     3601 ggttttcacc aagataaccg gcttcttcca tctccgcgat cttttcatcg agccatgcgg
     3661 ctacctgcca gaactcaccg cgcaaataaa tataggcggc atttgcgccg actgcataag
     3721 cacccagcgc cagaccttcc aaaaactgga agggattctt ttccatgatc tcgcggtctt
     3781 tgaaggtacc cggctcagac tcatctgcat ttgccacaat gtagtgcggc caagagttct
     3841 tatcaatgaa ggaccatttc atgccagtcg ggaagcccgc accgccacgt ccgcgcagac
     3901 cggagttctt gaccacatcg atgacatcat tcggctgcat cttggtgatg gcgttcttga
     3961 gagcagagaa accaccattc ttcttataga cactcatttt ttcgatatca gggatatcgc
     4021 gatggcgtaa aaggacgtaa gccatattta cttacctccc ttcaaagctt cgatcaattc
     4081 catagtacga tcaatggtca tatagtcgtg atatttgatg ccatccggtc cctgagtttg
     4141 gaacatgggg gcacggtcgc aagaggcgag acaagtcaca gcttcaatgg tgatgagacc
     4201 atcttcagtg gtctccccca ctttaacgcc gaggttctcg cacaatttat tcagaaactc
     4261 atctgagccg cgcaatgcgc aaggtaaatc gttgcaaact tgcatacgat atttccctgc
     4321 ttttttatcg tgatacaaag aatagaagcc gataatggaa gcgacttctg tctcggtaat
     4381 atcaagaatg gaggcgatgt ctttcatcga ctctttgttg acgtagcctt cctcgcgctg
     4441 agcaaggtaa agaagcggca tcaccgcaga gcgtttgtgc tctggcgggt atttggcaag
     4501 aatttgttta acttccttcg agtatttctt ttcaagactc atcggtcaat atctccgaga
     4561 acgatgtcaa tggaagcgag gatcgcaacc agatcggcaa ccaaatgtcc cttgacaatt
     4621 tcagggatga cggaaaggtt gtcgaatgat ggagtgcgca tgtggacgcg gcgcggcttg
     4681 ggtccgccat cgcctgccaa caacacaccg agttcaccgc gtggggattc cactgcgcta
     4741 taaatagaag catctggagc atggaaccct tcagtccaca acttgaagtg gtggatgagc
     4801 gcttccatgc ttacgccgat ctctgaacgg ggaggaggaa cgaacttgcg gttctccgat
     4861 cggacgggtc ccatcggtaa tttatccaac gcctgttcaa taatgcgcaa agactgacgg
     4921 aattcttcga tacgcaccaa gtaacgagcg taggtatcgc cttcggtcaa aaccggtaca
     4981 tcgaaatcat attgttcata gcccatgtaa gggcgggctt tgcgaaggtc ccagttgacg
     5041 cctgaggctc gcaagttcgg acccgttacc ccgtaggaaa gagccgtttc cttgctcagg
     5101 taaccaatat ccaccatacg atccacaaac agaggattct tcgtcaaaag tttttcatat
     5161 tcatcaatgc gcttaggaaa aatcttgatg aaatcgcgca cggctttttc aaattcaaca
     5221 ggcacatcgc gccacaaacc gccggggcgg aagaatgtgg tcatcatgcg ctgaccagag
     5281 acgagttcaa aaatatcgag ga
//
LOCUS       DAOQFT010000099         5293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7963_length_5293_cov_7.917144, whole genome shotgun sequence.
ACCESSION   DAOQFT010000099 DAOQFT010000000
VERSION     DAOQFT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7963_length_5293_cov_7.917144"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLR93_03805"
     CDS             <1..455
                     /locus_tag="PLR93_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH26115.1"
                     /translation="DFLLFLGIVFHLGISVRYMGKAELFNNPFGWFFYWCGGVPVDRK
                     KSVGLVDQMVEVCNQSDKFILVIAPEGTRYQVNEWKRGFYHIAKSANIPIVTARVDGI
                     QKIAHVGQIFQPGDDIEADMKAIQESFIGMTGINPRLKTKKKYITLAN"
     gene            456..911
                     /locus_tag="PLR93_03810"
     CDS             456..911
                     /locus_tag="PLR93_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNH26116.1"
                     /translation="MIIDRIENIALYVSLHPRFKQAFDYLSQIDIHAISVGRHDVDGD
                     AIYALVQEYTTKQTGEGKWEAHRRYIDLQYVVQGAEGMGYANIHHLQQGKYVPEKDFL
                     PLFGEGDQVPLRSGNFVLLFPEDAHMPNMALGEPAPARKIVLKIAVEAG"
     gene            complement(938..2542)
                     /locus_tag="PLR93_03815"
     CDS             complement(938..2542)
                     /locus_tag="PLR93_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26117.1"
                     /translation="MKEFFTQRIKRGLVRRLTLLKVASVAREVARKEPQPNGAPIVFF
                     KASTGIDDLSWNSGFHLLTTWALRLQGIPVAYFACNSGLSKCVLGTNRDNPHKEMPCK
                     SCVMQSKALYTGVEGHKSKVGWFDFQRDPELVSRISSLSVSELSTFHFQDLPLGELCL
                     PALRWILRIHHLNDDENTRYLLREYILSAWNVAQKFSKFLDETSPRLVVVFNGQFYPE
                     ATARYLAKKRGLRVITHEVGLEPATAYFTEGEATAYPIHIPDEFELSEAQNARLDAYL
                     AKRFKGDFTMAGVKFWADMKGLDEAFLQKAAQFKQIVPIFTNVIFDTSQPHSNTVFED
                     MFAWLDTTLEVIKEHPETLFIIRAHPDELRLRKASRETVGGWVESTRVTSLPNVVFVP
                     PNETLSSYELIAKSKFVMIYNSTIGLEASIMGAAVLCAGKARFTQYPTVFFPQTVEDV
                     RRKMKEFLTAETIKVPVEFKRNARRFLYYQLYRTSLPLGQFLEPSVRVTQTKLKTFDL
                     AELQKSKSMQAILDGLLNNGDFLLKE"
     gene            complement(2613..3467)
                     /locus_tag="PLR93_03820"
     CDS             complement(2613..3467)
                     /locus_tag="PLR93_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNH26118.1"
                     /translation="MGIKQKLRRALRPYAKTAQAIWHWKRFSFNDAPPIFANSKPKSG
                     SHLLLQVMNGFTQIMPYKYVDADPVRTITYKGRRKTNEEILADLKHTPKGVIGWGYVD
                     ATPENASFLTSAGRVNYFIYRDPRDMLVSQVFFATDMHEEHGMHNYYNSLPDFGARLN
                     VAITGIDRDGLKMVSVKQRYEGVFQWLEQKNTMCLRFEDLINNRDATLLKMLDEVEKT
                     GYKIPTPREKALPILVEAIQPKKSHTFRSGKTGGWAQHFTAEHKKLFKDVAGDLLVRL
                     GYEKNNDW"
     gene            complement(3516..4397)
                     /locus_tag="PLR93_03825"
     CDS             complement(3516..4397)
                     /locus_tag="PLR93_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNH26119.1"
                     /translation="MAREINFGKRKLGDGHPAYIIAEIGLNHNGDLDIAKKIIDAAVH
                     AGADAVKFQKRTPEVCTPPEQQKQMRETPWGYITYLDYRYKVEFNEEQYREIDRYCKE
                     KGIDWMVSVWDEPSVDFMEKFETPAYKVPSASLTDHNLLKYVRQTGKPVIISTGMSTM
                     EQIHKGVNAVGEDNLVIMHCTSTYPCEPEELNLKMIETLRKEFPNNPIGYSGHETGLV
                     PSAVAVALGACSVERHITLDRAMWGSDHAASVEPGGFEKLVKYIRVTEAGLGDGVKRV
                     YESEKAPMKKLRRVVNE"
     gene            complement(4400..>5293)
                     /locus_tag="PLR93_03830"
     CDS             complement(4400..>5293)
                     /locus_tag="PLR93_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNH26120.1"
                     /translation="PIRPKGMVDDAVNILLNHADADCVRGVVPAGQNPFKMWRFNSEE
                     KPLGQLLEVPGIAEPYNAPRQILPSVYWQTGHIDVIRTSTIVNKKSLTGNVIYPLVID
                     PKYTVDIDTLPDWAKYEAVIYSGLEVVSPGKSRRTMPKTIKLIVTDFDGVLSDGRVWV
                     DENGKETVAANRSDSMRIRQIRELGMDVIILSSEVNPVVSMRAKKMKVEAIHGMGLDE
                     KGEVLKKYLAEKHIDPAQVVYLGNDFNDLPCFEVAGWSVAVADAYPEVVRAADHVLNT
                     NGGFGALRELCDLILKEYKEN"
BASE COUNT         1355 a         1281 c         1251 g         1406 t
ORIGIN      
        1 gggatttttt gctgttcctt ggcattgtct ttcaccttgg cattagtgtg aggtatatgg
       61 gcaaagccga gttgttcaac aatccttttg gatggttctt ttattggtgt ggcggtgtgc
      121 ctgtggacag aaaaaagtct gttgggctgg tcgatcaaat ggtggaagtt tgcaaccaat
      181 ctgataaatt tattttggtc atcgcccccg aagggacgcg ttatcaggtc aacgaatgga
      241 agcgtgggtt ttatcatatc gccaagagcg ccaacattcc cattgtgacg gcaagggtgg
      301 atggcattca aaagattgca catgtgggtc agatatttca gcctggtgat gatattgaag
      361 cagatatgaa agcgattcag gaatctttca ttggcatgac gggcatcaat ccacgcttaa
      421 agacaaagaa aaaatatatt acattggcaa attaaatgat catagaccgc attgaaaaca
      481 ttgccctata tgtttcgctt catccgcgtt tcaagcaggc atttgattac ttgagccaga
      541 tcgatattca tgcgatatcc gttgggcggc atgatgtaga tggtgacgcc atctatgctt
      601 tggttcagga atacacgacc aaacaaacag gggaaggaaa atgggaagct catcgccgct
      661 atatcgactt gcaatatgtg gtgcaaggcg cagaaggcat gggctacgct aatatccatc
      721 atctacaaca agggaaatat gtacctgaga aggactttct acctttattt ggcgaaggtg
      781 atcaagtgcc gttaagaagt ggaaactttg tattgctatt ccccgaagat gctcatatgc
      841 ccaatatggc attaggtgag cctgcacctg ctagaaagat tgttttgaaa attgcagtag
      901 aggcggggta ataccgcttc gtggcacaaa ccccgcccta ctcttttagt aagaaatccc
      961 cgttattcaa caagccatct aagattgcct gcatagactt agatttctgt agctcagcca
     1021 aatcaaacgt ctttaacttc gtttgagtca cacgcaccga cggctcaaga aattgaccta
     1081 agggtaaaga ggttcgatat aactgataat acaagaagcg acgtgcgttg cgtttgaatt
     1141 cgacagggac tttgatcgtc tcagccgtca aaaactcttt catctttctt cgtacatcct
     1201 ctactgtttg cgggaagaat acggtgggat attgagtaaa acgcgcctta ccagcacaca
     1261 agaccgcagc ccccataatg gacgcttcca agccaatggt cgagttatag atcatgacaa
     1321 atttagattt cgcgattaat tcataggaac ttagcgtttc gtttggcgga acaaaaacaa
     1381 cattcggcaa actggtgact cgggtagatt ccacccatcc gcccactgtt tcacggctgg
     1441 ctttgcgaag acgtagctca tcggggtgag cgcggataat gaacaaggtc tctgggtgtt
     1501 ctttgatgac ctccagagtc gtatctaacc aagcaaacat atcttcaaag accgtgttgg
     1561 aatgcggctg gctggtatca aagataacat tagtaaagat gggcacgatt tgtttgaact
     1621 gagccgcttt ttgaaggaag gcttcgtcca gtcctttcat atcagcccag aatttgacgc
     1681 ccgccatcgt aaagtcacct ttgaagcgtt tggcaagata agcatcgagc cgtgcatttt
     1741 gcgcctcgct tagctcaaat tcatcgggga tatgaatcgg gtaggctgtc gcttcgcctt
     1801 cggtgaaata ggcagtagca ggctccaagc ccacctcatg ggtgatgacg cgcagtccgc
     1861 gttttttggc gagataccgt gccgtcgctt cggggtagaa ctgtccattg aagaccacca
     1921 ccaaacgtgg acttgtctca tcgagaaatt tcgagaactt ttgcgccaca ttccatgcgg
     1981 acaaaatgta ttcacgcaaa agataacgcg tgttttcgtc gtcattcaag tgatgaatac
     2041 gaaggatcca gcgcagggcg ggcaagcaca attcaccgag aggaaggtct tgaaaatgga
     2101 aagtggaaag ttcagagaca gaaagtgatg agatacgaga aacgagttca ggatcacgct
     2161 gaaaatcaaa ccaaccgact tttgacttgt gaccttcgac tcctgtataa agcgccttgc
     2221 tctgcatcac acaagattta caaggcattt ctttatgggg attatcgcgg ttcgtaccca
     2281 atacgcattt gctcagccct gagttacaag caaaatatgc cacagggatg ccctgcaagc
     2341 gcaaagccca agtggtcaac aaatggaacc cactgttcca tgagaggtca tcaatgccag
     2401 tggaggcttt gaagaataca attggtgcgc catttggttg cggctcttta cgagccacct
     2461 ctcgcgcaac ggatgcaact ttgaggaggg tgagtctgcg gacgagtccg cgtttgatgc
     2521 gttgagtgaa gaattctttc atagtcgtgc aaattgtatc agagagcagt acataaaaaa
     2581 tgggactgaa tttctccagt cccatgcata tactaccagt cgttgttctt ttcgtaccct
     2641 aacctgacca gcaaatctcc tgctacatcc ttgaaaagct tcttatgttc tgcggtgaag
     2701 tgctgagccc aaccgccggt tttgcccgag cggaaggtgt gacttttctt tggctgaatg
     2761 gcttccacca aaataggtaa tgctttttca cgcggagtgg ggatcttgta gccggtcttt
     2821 tccacttcat cgagcatttt caacaaggtc gcatcgcggt tgttgatgag gtcttcaaaa
     2881 cgaagacaca tcgtattttt ttgctccaac cactggaaga cgccttcata cctctgcttc
     2941 acgctaacca tcttcagccc gtcacggtca atgccggtga tggcaacatt caaacgtgcg
     3001 ccaaaatcag gcagggaatt gtagtaattg tgcatgccat gttcttcatg catatcggtc
     3061 gcgaagaaga cttgtgagac gagcatatct ctcgggtcgc gataaataaa atagttcacg
     3121 cgcccggctg aggtcaagaa agaggcgttt tctggggtgg catccacata gccccaaccg
     3181 atcactccct ttggggtgtg cttcagatcc gcaagaattt cttcgtttgt ttttcttctg
     3241 cctttgtagg tgatcgttcg cacggggtcg gcatccacat acttgtaagg catgatctgc
     3301 gtgaagccat tcatcacctg caaaagcaaa tgcgaaccgc tcttgggctt tgagttggca
     3361 aagatgggcg gagcgtcgtt aaacgaaaac cgcttccaat gccagatggc ttgggcggtc
     3421 tttgcatacg ggcggagtgc gcggcggagt ttttgtttaa ttcccataat ttaataaata
     3481 taggggcgag atttacctcg cccctacaaa attctttatt cgtttaccac acggcgaagt
     3541 ttcttcattg gagctttttc agattcatac acacgcttca cgccatcacc aagcccggct
     3601 tcggtgacac gaatgtattt gaccagcttc tcaaaaccac cgggttccac agaggcggca
     3661 tggtcactgc cccacatggc gcggtctagg gtgatgtggc gctccacgga gcaggcgccc
     3721 agcgccaccg ccacggcaga aggcacaagc ccggtttcat gcccagaata cccgattggg
     3781 ttattgggga attccttgcg cagggtttcg atcattttga gatttaattc ttccggctca
     3841 cacggatagg tgctggtgca gtgcatgatg acgaggttat cttcaccgac agcatttaca
     3901 cccttgtgaa tttgctccat tgtggacatg cccgtggaaa tgataaccgg cttgccagtt
     3961 tggcgcacat atttgagaag gttatggtct gttaaagacg cggagggaac tttataagcc
     4021 ggggtttcaa acttttccat gaaatccaca gagggctcat cccatacaga gaccatccaa
     4081 tcaatgcctt tttctttgca atagcggtcg atctcgcgat attgttcttc gttgaattcc
     4141 actttgtagc ggtaatccaa ataagtgata tagccccagg gcgtctcacg catttgtttc
     4201 tgttgttcag gtggtgtaca aacttcaggc gtgcgtttct ggaatttcac cgcatccgcc
     4261 ccggcatgca cggcagcatc gataattttc ttggcaatat ccaaatcacc gttatggttg
     4321 agcccgatct cagcaataat atatgcgggg tgtccatcgc ctaatttacg ttttccaaaa
     4381 tttatttcac gagccattct taattctcct tatactcttt caaaattaag tcacataatt
     4441 cgcgcagcgc cccaaagccg ccgttggtat tcaacacatg gtcagcggca cgcaccactt
     4501 cagggtatgc atctgccacg gcaaccgacc agcccgcaac ttcaaaacaa ggcaggtcat
     4561 tgaaatcatt tcccaaatac accacttgcg ccgggtcaat atgtttctcg gcaagatatt
     4621 ttttcaacac ctcacctttt tcatccaaac ccatgccgtg gatcgcctcc accttcattt
     4681 tcttggcacg catactcaca accggattca cttcagaaga caggatgatg acatccatgc
     4741 ccagctcacg tatctgacgt atccgcatcg aatcggaccg gtttgctgcc actgtttcct
     4801 taccattttc atccacccaa actcgtccat cgctcaacac gccatcgaag tcagtgacga
     4861 tgagtttaat agtctttggc atcgtccgac gtgatttccc cggcgagacc acttccagcc
     4921 cgctataaat caccgcttca tactttgccc aatcgggcag agtatcaata tccactgtat
     4981 atttggggtc gatcaccagc ggatagataa cattaccggt tagagacttt ttatttacga
     5041 tggtcgaagt acgaatcaca tcaatgtgac ccgtctgcca atacactgac ggcagtattt
     5101 ggcgcggagc attataaggt tcagcaatgc cgggtacttc cagcagttga ccgagcggtt
     5161 tttcttcgct gttgaagcgc cacatcttga aagggttttg ccctgcaggc acaaccccgc
     5221 gcacacaatc ggcatcggca tggttcaaga gaatattaac cgcatcatcc accattcctt
     5281 tcgggcggat ggg
//
LOCUS       DAOQFT010000100         5283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_7990_length_5283_cov_4.183053, whole genome shotgun sequence.
ACCESSION   DAOQFT010000100 DAOQFT010000000
VERSION     DAOQFT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7990_length_5283_cov_4.183053"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PLR93_03835"
     CDS             <1..1161
                     /locus_tag="PLR93_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH26121.1"
                     /translation="LLDAKEIGKGSGSGNAGHIVPSHIIPLAAPGVVTSALKWMLDPT
                     HSPFGMKVSLDPNYLMWLLRFTLACSQANVLRNIDPLNELGQLSAKNFAQIIAEEKFD
                     CAYQEMGLLHLFKTEKAFHEGQHEGEFMLKHGIPVSVYDKNKIHEVEPAAVDDVIGGV
                     HFTGDAHLNPMVFLKLLKERVKSMGAELMDGTQVTGFETANGKVTKIKTTAGDFEAEQ
                     IVLAAGALTPFVAKDLKLNIPIQPARGYSLTMTANKIMPSHALILGERRIAVSPMAGL
                     LRFTGRLEVGNYSMEPNPVWIQRIENSAREYLRLDEKLDVKETWAGLRPTTPDGVPII
                     GKSPKHSNLVLATGHAMLGLSLGPGTGLVVSQLLNGQGPAFSLSAMRLERFG"
     gene            1267..2241
                     /locus_tag="PLR93_03840"
     CDS             1267..2241
                     /locus_tag="PLR93_03840"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNH26122.1"
                     /translation="MKRMMILLFGMCLLSACGGAEPPLEPTRTPEPTQIPTLEPTMTA
                     IPEPQFEVETFAGDMKDPYGIAVNSLGNVYVSDAGNNRVLVFDATGNLISNWDKQGSG
                     DGEFKTMGFGGLTVDANNNVFVVDNGNHRIQKFDKDGNFIAQWGSEGTEDGQFVRAIG
                     IAVDAEGNVYVTDDGNPYVQKFDNDGNFLMKFGGAGEADGRFKHATGIAVDAQGNIFV
                     ADYEAKRMQKFNASGGFLTSWKMGEDIAISGIPEGIAVDPDGNVYVTDYNLGRVQVFD
                     NDGNFLWAIGSKKLSENPFKRPTAIAFGNGVMYVVNQSGANVSVMELP"
     gene            2439..3317
                     /locus_tag="PLR93_03845"
     CDS             2439..3317
                     /locus_tag="PLR93_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH26123.1"
                     /translation="MEFIYHFFDKLIIDLKNLFYVVALFLFQYFLAWLFFIPFSILFF
                     ISPEEWGQNESLVVAHEIGTLFMITGSVAIVSKIVREMELKDFGLERNFQALKDFFAG
                     LTITLIVLGMGFLFLLVIGMVNVTGFAWSHMPISSLLLYLLATFIIFFLVGWNEELLS
                     RGFHLQIIESKFGKFIAVLVSSVIFSYMHRFNIGIDLSSHVFIFFAGSMLAYAYLKTR
                     SLWLPIGLHAGWDFFVVVGFYGVPLNDIKIFNLIDISISRGPFSGPFLYVIQYIELAL
                     IVYLANWYSRQARNTI"
     gene            3342..4508
                     /locus_tag="PLR93_03850"
     CDS             3342..4508
                     /locus_tag="PLR93_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26124.1"
                     /translation="MGSSFFIILVSVFVDMLGYGIMLPLLPFFVQAQDGGAAIAGGLM
                     SMYSTIQLVSGPILGALSDRYGRKPILLLCLLGTATSYLLLGLANSLTIIFVAVFIDG
                     LTGSNLTLAHAYVADTTTAENRAMGINYSQLAFGLGIMAGPILGGLLSGYGLSVPALV
                     ASFLAFANTAFGFFFLPESLSPERRETKPISQVFSWAGQFTSIFRQKNIQHFLVVLFL
                     LNLAFAGLQTNFPLFSNARFGWTPAQNSYFYLYVGLCGVIVQGILFARLQARFGESKL
                     IPAGLLFVAIGLAGMAFVPDAWMIFPAVAVVALGTGVSIPSLTALVSLRVPENEQGRL
                     MGGNQTLLALTNIFGPTIAGISFDVIAISAPYWLGSLFSLFALSVAIIALRKKR"
     gene            complement(4505..>5283)
                     /locus_tag="PLR93_03855"
     CDS             complement(4505..>5283)
                     /locus_tag="PLR93_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH26125.1"
                     /translation="NWLSHWAVTQATWLSWRSEINHARHTDFGLAPAHWLDPSLQADS
                     TQPLTLNAFSEIVVPRPKDWNAKQLVTGYWRIKEETSERDDEFEGIQRFIDSAKNPIA
                     IGFGSPGTQGYLPMLEEALHLANAQAVLTLPAKRHSNVKSNNIFPIEYISHDWLYKRV
                     KVAIHHGGAGTTSASLHAGIPTVALPLAIDQFFWGERIQKLGVGVSIAQRKLNAENLA
                     QGIIEALNNSSMQSKAKSIHEKLNKEDGVQAATKELQKII"
BASE COUNT         1227 a         1157 c         1360 g         1539 t
ORIGIN      
        1 ctgctcgacg cgaaagagat cggcaagggc agcggctcgg gcaatgccgg gcatattgtg
       61 ccgagtcata tcattccgct cgccgcgccc ggcgtggtga cctctgccct caaatggatg
      121 ctcgacccca cacacagtcc gtttgggatg aaggtcagcc tcgacccgaa ttatttaatg
      181 tggctgttgc gtttcactct agcgtgtagc caagccaatg ttttgcggaa tatcgatcca
      241 ctaaatgaac ttggacagtt gagcgcgaag aattttgcac agatcatcgc cgaagaaaaa
      301 tttgattgtg cttatcaaga gatggggctg cttcatctct tcaagaccga gaaagccttt
      361 catgaaggac agcacgaagg cgagttcatg ctcaagcatg gaatccctgt cagcgtgtat
      421 gacaaaaata aaattcacga agtcgaaccc gccgcagtgg acgatgtcat tggcggtgtg
      481 catttcacgg gagacgcgca tctcaacccg atggtgtttc tcaaattgct gaaggaacgc
      541 gtcaagtcta tgggcgcgga gttgatggat ggcacacaag tcactgggtt tgaaactgcg
      601 aatggaaaag tgaccaaaat caaaaccacg gctggagatt ttgaagccga gcaaattgtc
      661 ctcgctgcgg gcgcattgac tccattcgtt gccaaagatt tgaagttgaa cattcccatc
      721 caaccggcac gcggatacag cctgaccatg actgcaaata aaatcatgcc gagccatgcg
      781 ttgatcttgg gcgaaagaag aatcgccgtc tcgccgatgg ctgggctgct gcgtttcacg
      841 ggtcgtctgg aagtaggcaa ttacagcatg gagccgaacc cggtgtggat tcagcgcatc
      901 gaaaactctg cgcgagaata tttgcgcctc gatgaaaaac tggatgtgaa agaaacctgg
      961 gcgggtttgc gaccaacgac gcctgatggt gtgccaatca ttggcaagtc accaaagcat
     1021 agcaatcttg tgctggcaac gggtcatgcc atgctgggtc tctcgttagg accgggaacc
     1081 ggcttggttg taagtcaact gctcaatgga cagggaccag ctttcagttt aagtgcgatg
     1141 cggttggagc ggtttgggta aattctgtta atgcgtcaaa agtagcgggt gaatccattc
     1201 tttagtatgg ggattcatta gttctgcgcg tctataatcc gcaaaacttt ataaggagga
     1261 acgtgcatga aaagaatgat gatcctactt tttgggatgt gcttgttgag cgcttgtggt
     1321 ggtgcggaac cacccctcga accgacccgc acgcctgagc cgacccaaat tcccaccctg
     1381 gagccaacca tgaccgccat ccctgaaccg caatttgaag ttgaaacatt tgctggtgat
     1441 atgaaagatc cctatgggat cgctgtgaat tctcttggga atgtatatgt gagtgacgcg
     1501 gggaataatc gagttttggt attcgatgcg accgggaatt tgatctcaaa ctgggataag
     1561 caaggcagcg gtgatgggga attcaagacg atggggtttg gcggcctgac cgtcgatgcg
     1621 aacaacaatg tctttgtggt ggataacggt aatcatcgca ttcaaaagtt tgataaagac
     1681 ggaaatttta ttgctcagtg gggcagtgaa gggacggagg atggtcagtt cgtgcgggcg
     1741 attggcattg cagtggatgc ggaggggaat gtgtatgtga ctgacgatgg caatccgtat
     1801 gtgcagaagt tcgataacga tggtaatttt ctgatgaagt tcggcggcgc tggcgaggcg
     1861 gatggacgat tcaaacatgc gaccgggatc gccgtggatg cacagggaaa tatcttcgtt
     1921 gcagactatg aagcgaaacg catgcagaag ttcaacgcaa gcggcggctt tttaaccagc
     1981 tggaagatgg gtgaagatat tgcaatctcc ggcattcccg aagggatcgc tgtggatcca
     2041 gatgggaatg tctacgtgac cgattacaac ctgggacgtg ttcaggtatt cgacaatgac
     2101 gggaactttc tgtgggctat cggtagtaag aagctgtctg agaatccctt caaacgcccg
     2161 accgcgattg cgtttggcaa tggtgtgatg tatgtagtca atcagtctgg cgcgaatgtt
     2221 tcggtgatgg aactgccgta gcaaaaaatc aataacaact tttgaattca agtgtttttg
     2281 ttgcagggtg gatatttcga gcccgtgtta aattaaataa tcacgtataa tctgcgggac
     2341 gttataaacg tcccgcagat taaattaacc attaaatgca aaagcagttt atagtctgga
     2401 caaaaatagt aatcattcca aatcgggaga catttatcat ggaattcatc tatcattttt
     2461 ttgataagct gattattgat ttgaagaacc ttttttatgt agtggctttg tttttatttc
     2521 aatatttttt agcttggttg tttttcattc cttttagtat tctgtttttt atttcacccg
     2581 aagagtgggg acaaaatgaa agcttggttg tcgcccatga aattggtaca ttattcatga
     2641 ttactggctc tgtcgcaatt gtctcaaaga ttgtaagaga gatggaattg aaagattttg
     2701 gactggagag aaattttcag gctttgaagg atttttttgc aggtttaaca attacgttaa
     2761 ttgttttagg aatggggttt cttttcttac ttgtaatagg tatggtaaat gttactgggt
     2821 ttgcttggtc acatatgcca atctcctccc tgcttttata tttgttggcc acgtttatca
     2881 ttttttttct tgtcggctgg aatgaagaac ttctatcgcg cgggtttcat ttgcaaatca
     2941 ttgaaagtaa atttggtaaa tttattgcag tcctggtttc ctctgttata ttttcttaca
     3001 tgcataggtt taatatagga atcgatctgt caagccatgt gtttatattc tttgctggtt
     3061 cgatgcttgc ttatgcatac ctaaaaacca gaagcctttg gcttccaatt ggacttcatg
     3121 ctggctggga cttctttgtt gtggttgggt tttatggggt tccgttgaat gatatcaaga
     3181 tatttaattt gatagatata tccatttcta gagggccatt ttctggtcca ttcttatatg
     3241 tgattcaata tattgagttg gctctgattg tgtatcttgc aaattggtac tcccggcagg
     3301 ctagaaatac aatttaaccc atccaatcaa aggtgaacag attgggctct tcttttttta
     3361 tcatccttgt ttccgttttt gtcgatatgc tcggctacgg cattatgctg cccttgctgc
     3421 ctttctttgt gcaggcacag gatggaggtg ctgccattgc aggcggtttg atgtcgatgt
     3481 actcgaccat tcaactcgtc tcaggtccca tcctcggcgc gctctccgac cgttacgggc
     3541 gcaaacccat tttgctgctc tgtctcctcg gcacggcaac atcctatctt ttgcttggct
     3601 tggcaaattc actgaccatt atttttgtcg ctgtttttat tgatggtctc accggctcaa
     3661 acttaaccct tgcgcatgcc tacgttgcag atactacgac ggctgaaaac cgagccatgg
     3721 gcatcaacta ttctcaactc gcttttggat tgggcatcat ggcggggccc atcctcggcg
     3781 gactcttgag tggatacgga ctcagcgtcc cagcgctcgt cgcttccttc ttagcctttg
     3841 ccaataccgc cttcggattc tttttcctcc ccgaatcctt atcccccgaa cgccgcgaga
     3901 ccaaaccgat ctctcaagtc tttagttggg ctggtcaatt cacaagcatc ttccgccaga
     3961 agaacatcca acattttctc gtcgttcttt tcctgctcaa cctcgccttc gccggtttgc
     4021 agacgaattt tccgcttttc tccaatgccc gcttcggttg gacacctgcc cagaatagtt
     4081 atttttattt gtatgtcggc ttgtgtgggg tgatcgtgca gggaatttta ttcgccagat
     4141 tgcaggcgcg ttttggcgag agtaaactga ttcctgccgg attgctcttt gtggcgatcg
     4201 gcttggcagg tatggcattt gtacccgatg catggatgat tttccccgct gtggctgtcg
     4261 ttgcgttggg gacgggtgtc agcatcccat ctctgacggc gctggtctcg ctgcgcgttc
     4321 ctgaaaatga gcaaggacgt ctgatgggag gtaatcaaac cttgctggcg ctgacgaata
     4381 tttttggtcc aacgattgca gggatttcgt tcgacgtcat tgccatctcc gcgccgtatt
     4441 ggttggggag tttgttttct ctttttgctt tgagtgtggc aattattgct ctacgaaaga
     4501 aacgctaaat tattttctgc aattcttttg ttgctgcctg aaccccatct tccttattta
     4561 acttttcatg aatagacttg gctttcgatt gcatggaact attattcaat gcttcgataa
     4621 ttccttgggc tagattctct gcattgagct tcctctgcgc aatggacacg cccacaccca
     4681 gtttttgaat ccgctcgccc cagaagaatt ggtcaatggc gaggggcaat gcgacggtgg
     4741 gaatacccgc atgcaaactc gcggacgtgg tccctgcccc gccgtggtgg atggcgactt
     4801 tgacgcgttt gtacagccaa tcgtgtgaaa tgtattcgat ggggaagatg ttattggatt
     4861 tcacgttact gtgccgcttt gcagggagag tcaacacggc ttgagcgttt gcgagatgca
     4921 aggcttcttc aagcataggt aaatatccct gcgtgccagg gctgccgaat ccgatagcga
     4981 tgggattctt tgctgaatcg ataaaacgtt gaatgccttc aaactcgtca tctcgttcac
     5041 tcgtttcttc ttttattcgc cagtaaccag tgaccagttg ttttgcattc caatctttgg
     5101 ggcgtggcac gacaatttca ctgaaggcgt tgagggtgag cggttgggta gagtccgctt
     5161 gcagtgaagg gtcaagccaa tgggcaggag caagcccaaa gtccgtgtga cgcgcgtggt
     5221 ttatttccga gcgccacgaa agccaggtgg cttgagtcac agcccaatgc gagagccagt
     5281 tcc
//
LOCUS       DAOQFT010000101         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8068_length_5253_cov_6.396883, whole genome shotgun sequence.
ACCESSION   DAOQFT010000101 DAOQFT010000000
VERSION     DAOQFT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8068_length_5253_cov_6.396883"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..429
                     /locus_tag="PLR93_03860"
     CDS             127..429
                     /locus_tag="PLR93_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNH26126.1"
                     /translation="MTTDPTFRMTVQDIFFIKGRGTVVTGRVESGIITVGDEIRIQGK
                     SANKTAIVTGVEVLRKVTTQAKAGDNIGLLFKDITKEDVQVGDILTGSDLDFTWKP"
     gene            466..1077
                     /locus_tag="PLR93_03865"
     CDS             466..1077
                     /locus_tag="PLR93_03865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNH26127.1"
                     /translation="MKEEIHITFLRHGRSRADDENVHEGRYDSPLTDVGRSQVQARVQ
                     DFLSRQFHFDAAISSPLQRAHETATIIGQALSLPIETDADWMEMDNGPLAGMPWDEAE
                     KQYPKPAFRNPYEPFCGSGESDWEIYNRGAKAVEKIIRRGPGSYLVVAHGGILNSALR
                     TIIGTQPSINQQGITFGFGDTGYARFSYRPAQHLWYLLEFKME"
     gene            1106..1642
                     /locus_tag="PLR93_03870"
     CDS             1106..1642
                     /locus_tag="PLR93_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26128.1"
                     /translation="MPTTSLTHRLAKREDLTALKALMDAAISENQKAFLDESQIASSR
                     TIMGLDTQLIDDGTYFIVEIDGQLAGCGGWSRRATLYGGDQTPGRSAALLDPAKDPAR
                     IRAMYTHPNHVRKGIGKLIITLCEEAAKAEGFTKMELVATLSGEPLYRTCGFEACENI
                     VDDRGGVGVPLLRMRKSL"
     gene            1720..3702
                     /locus_tag="PLR93_03875"
     CDS             1720..3702
                     /locus_tag="PLR93_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26129.1"
                     /translation="MQFRLSPIARQTLLIAVITIIAYGLLLPLTGFYWDDWPFAWIAK
                     FLGPAEFNPAFMPFRPFLGPIFYFTTTVIPLNPLAWQIFALVIRFLIGVTAWWTFTQI
                     FPQRKTLAFFAALLMLVFPGYSQHWVALTHINQELIPFLFYLLSFGYTFKALHTKKKT
                     DTVIALLLQLCGIFPTEYFFGVEGIRFFLLFFVFQGAILERFTKTLKVWLPYLLIWFL
                     NAAWLFYYYKFGPYESYEVAAAQSPNLFYFLTQALDALWKAGFYIWAQVLVLTASTLH
                     APASLLTLGLVAISFILLAWVLIRSTQAEATDKSFAISLLTIGFFGILLGRLPSLAAN
                     LPLTLQSSYDRFMISIMLGSVLFVLGLLELFIKDHRTRVFVLSALIALGIGQQFFNAN
                     IFRRDWQKQGEIYWQMAWRMPALAENTVLLTHQMPIDYETDLSFTAPINWMYAPNYTR
                     ANLPYMLLYTEKRLGGSTLPSLDKSTSITYPYRTVQFDSTLSNAIVIYMPQNGCLRVM
                     DPSRGHAEIYDNIADEFTQAIAISDPSRILVKSTPPAEPMFFPEPAKDWCYYFAMVEL
                     YNQWNEYQVSAEFADEALAAKFQPEDPREWIVFIEAYAMNGELDKAIDASNSALKMDK
                     KTLKAICTTWEQVQARGPERGAEGFESARLSLGCNP"
     gene            3770..4534
                     /locus_tag="PLR93_03880"
     CDS             3770..4534
                     /locus_tag="PLR93_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH26130.1"
                     /translation="MSNKRRSVLALSIFMITAVMLSACTQSLSSAPAETPTLLPTGLF
                     VSPIASVENPMEMIEQFAKQTEAAQTSAAGGGTPGTPGTPQAVSTITTGTPGTPAPAV
                     ASVTPSAPTVSVSTPTNANAAAPTAAVPTSSSSSAATAPSLSGNTYVLQDGEWPYCIA
                     RRYNVDPDAMLQASGLTSPDIYYAGLKLVLPQNTTFPGARVLIAHPASYTVASGDETV
                     YSIACKYGDVDPNALASANGISVSSKLTVGQVLSIP"
     gene            4608..5138
                     /locus_tag="PLR93_03885"
     CDS             4608..5138
                     /locus_tag="PLR93_03885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNH26131.1"
                     /translation="MPSFELIRSEPLMQGRAFKIRRDYLKTPDGHETKYDIVEHGGSV
                     VIVPFDGDGNLLFVRQYRHAAGMDMLELPAGTRDGDEPFEECAAREIREETGMEAGKL
                     IHIGSFYLAPGYSTEYMGVFLATDLKHNPLEADEDEFLSVEKIPVKDALKMAEKGEMP
                     DAKSLAALLMARAYLE"
BASE COUNT         1346 a         1453 c         1130 g         1324 t
ORIGIN      
        1 aaattgccgc aaaacttgaa cgagaaggac atgtaaaaca ctggcaagca gaagcccaag
       61 cctttctaga aaatcgaaat cgcttcgcta aaaatctgtc tcagcccgat caataaagga
      121 ttccccatga ccacagaccc tacttttcgc atgaccgtac aagatatctt ctttataaaa
      181 ggacgcggaa ccgttgtaac tggtcgcgtt gaaagcggaa taatcacggt cggagatgaa
      241 attcgaatcc aagggaaaag cgccaacaaa acggctatag taacgggggt agaagtcctt
      301 cgcaaagtca caacacaggc aaaagctggt gacaatatcg gactactgtt caaagacatc
      361 acgaaagaag atgttcaagt tggggatatc ctaacagggt ctgatttgga tttcacctgg
      421 aagccataaa cgaccttatc cgatgttgtt attttgggag tactcatgaa agaagaaatc
      481 catatcacct tcctgcgcca cgggcggtct cgcgccgatg acgaaaacgt tcacgaaggc
      541 cggtacgact cacctctcac tgatgtaggg cgttcccaag tccaagcccg cgtgcaagat
      601 tttctctccc gacaatttca ttttgatgcg gctatttcaa gtccgttgca acgagcacat
      661 gaaaccgcca cgatcattgg gcaagcgtta agtctcccca ttgaaacgga tgccgattgg
      721 atggaaatgg ataatggtcc cttagctggc atgccttggg acgaagccga aaaacaatat
      781 cccaaacctg catttcgaaa tccatatgaa ccgttttgcg gttcaggaga aagcgactgg
      841 gagatttaca atcgcggagc caaagctgta gaaaaaatca ttcggcgcgg acccggaagt
      901 tatttagtcg tcgcccatgg tggcattctc aattctgctt tacggacaat tatcggcaca
      961 caaccatcta tcaaccaaca gggcatcaca tttggttttg gtgatactgg gtacgcccgc
     1021 tttagttatc gtcctgctca acatctatgg tatctcctcg aattcaaaat ggaataaaca
     1081 cactgaaatt aaaaaaggaa gccccatgcc cacaacctct ctaacccaca gacttgccaa
     1141 gcgagaagac ctgacagccc tcaaagcctt aatggacgcc gctatctcag aaaatcaaaa
     1201 agcctttctc gatgaaagtc agatcgcttc gagccgtacc attatggggt tggatacgca
     1261 actcatcgat gacggcacct acttcatcgt cgaaatcgat ggtcaactgg caggctgcgg
     1321 cggatggagt cgacgtgcca ccctgtatgg tggtgaccaa accccagggc gcagtgccgc
     1381 cctgctcgac cccgccaaag acccggctcg tatccgcgcc atgtacacgc atcctaacca
     1441 cgtccgcaaa ggaatcggga aactcatcat caccctatgt gaagaagccg ccaaagcaga
     1501 aggcttcaca aaaatggaat tggtcgccac cttgtctggc gaaccactgt atcgcacctg
     1561 cggattcgaa gcttgcgaaa atatcgtcga cgatcgcggt ggtgtgggcg ttccgctctt
     1621 gcgaatgcga aaatcgctgt aaaaattttg tattccttat ctctacatct cacatggcaa
     1681 gaattaagac tcaacctcgc catcccttat aattaaggga tgcaatttcg cctctccccc
     1741 atagcacgtc aaaccttgct cattgcggtt atcaccatca tcgcctatgg gttgctctta
     1801 ccgctcactg gcttctactg ggacgactgg cccttcgcat ggatcgccaa atttcttggt
     1861 cctgccgagt tcaacccggc gttcatgccc tttcgcccgt ttttaggtcc tatcttttat
     1921 ttcactacta ctgtcattcc gcttaacccg ttggcatggc aaatctttgc gttggttatc
     1981 cgctttctca tcggtgtcac cgcctggtgg accttcactc aaatcttccc acaacgtaaa
     2041 acacttgcat tcttcgcggc tttgctcatg ctggttttcc ccggttatag ccaacattgg
     2101 gtggcgctca cacacatcaa ccaagagctc atcccctttc tgttttatct actttctttc
     2161 ggctacacct tcaaagccct gcataccaag aaaaagacag atacggtcat cgcattactt
     2221 ttacaactat gcggcatctt ccccaccgaa tatttcttcg gcgtcgaagg tattcgcttc
     2281 tttttgctct tcttcgtttt tcaaggcgcc atcctcgaac gcttcacgaa aacactcaaa
     2341 gtctggctcc cctatttact tatctggttt ctcaatgccg cctggctttt ctattactat
     2401 aaattcggtc cctatgaatc ctacgaagtt gccgccgcgc aatcgcctaa tctgttttac
     2461 tttctaacac aagcgctgga tgctctctgg aaagccgggt tctacatttg ggctcaagtc
     2521 ctcgtcttaa ccgcttccac cctacacgcg cccgcctctc ttctgacctt gggcttggtc
     2581 gccatctcat tcattttgct cgcctgggtc ttaatccgct ccacccaagc cgaagcgaca
     2641 gataaatcct ttgccatctc cttactaaca attggcttct tcggtattct cttagggcgc
     2701 cttccctctc ttgcagccaa tcttccccta actctgcaat cctcctacga ccgtttcatg
     2761 atctccatca tgctcggttc ggtcttattc gtgttaggtc tgctagagtt attcatcaaa
     2821 gaccatcgca cacgtgtctt tgtcctttct gccctcatcg ccctcggtat tgggcagcaa
     2881 ttcttcaacg ccaacatctt ccgcagagat tggcaaaagc aaggagaaat ctactggcaa
     2941 atggcatggc gcatgcccgc attggcagag aataccgttc tcctcaccca ccaaatgccc
     3001 atcgattacg aaaccgacct ctccttcacc gcgcccatca actggatgta tgcgccgaac
     3061 tatacccgtg ccaatcttcc ttatatgttg ctttacacag aaaagcgttt gggcggttct
     3121 accttaccgt cattagataa atctacatcc atcacctacc catatcgaac tgtacaattt
     3181 gatagcacac tctcgaatgc catagtgatt tatatgccgc agaatggctg tctacgcgtc
     3241 atggacccaa gccgtggtca cgccgaaatt tacgacaaca tcgccgatga attcacccaa
     3301 gccattgcaa tctcagaccc atcgcgcatc ttggtcaagt ccacgccgcc agccgagccg
     3361 atgttcttcc ctgagccagc caaagactgg tgctattact tcgccatggt ggaactttac
     3421 aaccaatgga acgaatacca agtctctgcc gagtttgcag atgaagcgct ggcagccaag
     3481 ttccaacctg aagacccacg cgagtggatc gtcttcattg aagcgtatgc catgaacggc
     3541 gaactggata aagccattga tgcatctaac tctgctctta agatggataa gaaaacgctc
     3601 aaagcgatat gcacaacttg ggagcaggtg caggcacgag gtccagaaag aggcgcggag
     3661 gggtttgaat ccgctcgatt gtcacttggt tgcaatccat aacgctggct tgacgcttta
     3721 aacttcatgt gctaccatct ttttttagta aatagaacga ggagaatcta tgtctaataa
     3781 acgccgcagt gtgctggcat tgtcgatctt tatgatcaca gctgttatgc tctccgcctg
     3841 cacacaatca ctttcgtctg ctcccgcaga aacaccaact ctcttaccaa cagggttgtt
     3901 tgtttcgccg attgcttctg ttgaaaaccc aatggaaatg atcgagcagt tcgccaagca
     3961 gaccgaagct gctcaaacat ccgccgctgg cggtggcacc ccgggcactc ccggaacccc
     4021 acaagcagtt tctacaatca ccacaggcac ccccggaaca cccgcaccag ccgtcgcttc
     4081 tgttacccct tcggctccta cggtgagtgt ttccaccccc accaatgcaa atgcagcggc
     4141 tcctactgcc gcagtgccaa cttcttcatc tagctctgct gcaacagcac ccagcctttc
     4201 tggcaacacc tatgttctgc aagatggtga atggccttac tgtattgccc gccgttacaa
     4261 cgtggaccct gacgccatgc ttcaggcaag tggtcttacc agcccagata tttactatgc
     4321 tggtttgaag ttggtccttc cgcagaacac caccttcccc ggtgctcgcg ttttgatcgc
     4381 gcacccggct tcgtacacag tcgcttcggg tgatgaaaca gtgtacagca tcgcttgtaa
     4441 atatggtgat gtagacccga atgcactcgc gagtgcgaat ggaatttctg tctcttccaa
     4501 gttgacggtt gggcaggttc tgagcattcc gtaatttcaa aaagacaaaa gcccaggtca
     4561 acagcctggg ctttttttat agaaactatt cttgaaagga aatcactatg ccatcatttg
     4621 aactcattcg ttctgaacct ctcatgcagg gacgtgcctt caagattcgc cgtgattatt
     4681 tgaaaacgcc agatggtcac gaaaccaaat acgatattgt agaacatggc ggctcggtgg
     4741 tgatcgtgcc atttgacggg gatgggaacc tgctcttcgt gcgacaatac cgtcacgcgg
     4801 ctggtatgga tatgcttgaa ctgcccgctg gtacgcgcga tggtgatgaa ccatttgaag
     4861 aatgtgccgc acgtgagatc cgcgaagaga caggcatgga agctggcaag ttgattcaca
     4921 tcggttcgtt ctacctcgca ccgggctact ccactgaata catgggcgtc ttcttagcaa
     4981 cagatttgaa acacaatcca ctagaagccg atgaagatga attcctaagc gtagaaaaaa
     5041 ttcccgtcaa agatgcattg aaaatggcag agaaaggcga aatgccagat gcaaaatcat
     5101 tggctgcatt gctaatggca agagcttatc ttgaataaac cgtttgaaaa ataacaatcg
     5161 ctcaaaaaac gtcaaaacac ctcgcaaacg tcgaggtgtt ttatttccac caatcctatc
     5221 caatatatga catcaggcag ggggatttaa gtc
//
LOCUS       DAOQFT010000102         5219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8167_length_5219_cov_8.104376, whole genome shotgun sequence.
ACCESSION   DAOQFT010000102 DAOQFT010000000
VERSION     DAOQFT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8167_length_5219_cov_8.104376"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLR93_03890"
     CDS             <1..446
                     /locus_tag="PLR93_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26132.1"
                     /translation="TKIPAIYVTHDQEEAFTIADRILVLHEGTIIRDAAPHEIWREPK
                     SAFVAKLLGIGNVIDGEVVADKKVKTAFGVFALDCKKQVGEKAQLLITIEEERGEKIQ
                     LTVEDVLFKQDQFQVTARGGITIHMKEAPKVGDIINIKVQVKCLS"
     gene            443..871
                     /locus_tag="PLR93_03895"
     CDS             443..871
                     /locus_tag="PLR93_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNH26133.1"
                     /translation="MKILKKNLADEVTWQYEGEELSRDKNSITIEALFNRDDMPFQEI
                     VLKRNDRFVETFYSDKWYNIFAIYDRDDGKLKGWYCNITKPAIIEIDSVSYVDLALDL
                     WVSANGKQTVLDEDELEELKLGEALKENVYAGLRELQAFF"
     gene            complement(916..1068)
                     /locus_tag="PLR93_03900"
     CDS             complement(916..1068)
                     /locus_tag="PLR93_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26134.1"
                     /translation="MSEEMPVAPSETPAPNNNNRNIIIGVVAVVVLCCCCVLGSGIGY
                     YVWNNF"
     gene            complement(1115..1687)
                     /locus_tag="PLR93_03905"
     CDS             complement(1115..1687)
                     /locus_tag="PLR93_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNH26135.1"
                     /translation="MELLLGIFTAFGLSASAGLNAYIPLLVVGVIAHYAPNTLNLASP
                     FDLLANPWILILLGVLVIIEMVADKVPAVNHINDLIQTIIRPVAGAIAFAASANVITD
                     VNPVLALACGLLVAGSVHTVKAAAVRPAVTATTAGAGNIPVSIAEDVIATITSILAIL
                     IPLIAGTLFIIFLVLVLWWWASRKQKAQSA"
     gene            complement(1747..2595)
                     /locus_tag="PLR93_03910"
     CDS             complement(1747..2595)
                     /locus_tag="PLR93_03910"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNH26136.1"
                     /translation="MQTNPQPSKLETIASWVVPIAAIAAVIAWFIIAPEGALGKLDAI
                     GYAVCHRIDVRSFHIGDRQLPLCARCTGEFYAAGLTLLFQMVVSGKRSKLPSRGVMAV
                     LFGFFVAFGVDGTNSYLYLLKETGRNSGFLAQIPNLYVPNNVLRLFTGSGMGIALAAM
                     LYPVINQTLWRTLDDRPAIEWKQLGILVAGIIGINLLVLTDSPFVLYPIAYISALGTL
                     SLLVIVFGLLWLIIMKQDNSFEHPRQLWLAFASGLTLALLLILSIDLMRLNFTGTWSG
                     FPGLSG"
     gene            2686..3771
                     /locus_tag="PLR93_03915"
     CDS             2686..3771
                     /locus_tag="PLR93_03915"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNH26137.1"
                     /translation="MKRIGILTGGGDAPGLNAVIRAAVKTAINEYQCEVIGIRDGYDG
                     FLDTQNGIVPLGMEEVRGILPRGGTILGAANRGNPYARKVIRDGKEVTIDVSDEIVKG
                     IKELGLDALLVLGGDGTLHIAHELAQKGVPVIGVPKTIDNDIGGTEITFGFDTALNIA
                     TEALDRLHTTAESHHRVMVVELMGRDAGFITLNAGISGGADVILIPEIPFKFENIMKK
                     IQSRSGQGRKFSLIAVSEGAKPIGGMQVYSRKGDEVYVPRLGGIGQVVAEFIEQQGFE
                     SRVTVLGHVQRGGTPTAFDRGLATRYGAAAVRLAASGRFDRMVALQCGEITSLPLAEA
                     VAVPKRVDPQGDAVITAKNIGISFGDE"
     gene            complement(3807..4931)
                     /locus_tag="PLR93_03920"
     CDS             complement(3807..4931)
                     /locus_tag="PLR93_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNH26138.1"
                     /translation="MADIRVQKYAKILVEHSARVKPGDRILLEGTTAAEPLLRELYIQ
                     ILEKGGHPHLMMALPGTMPFSQDELYLTYANDAQLDFVPTFYKVAYEQFEGRIRIHSA
                     TNTKGTTNIDPAKSARRNKATGVITDFQFRRGATDEFKWCTTLYPTEAYAQDAGMSLM
                     EYEDFVFGAVHANEADPVAFWNSVKAKQQAAADFMKGKNQVILRGPNVDLTLSVKGRT
                     FINSYGTHNMPDGEIYTGPVEDSVNGWVKFTYPANYQGTSVEGAELTFSNGKVTQANA
                     TKNLDFLLKTLDSDDRSRYLGEFAIGTNFDIQKFTGNILFDEKIGGSFHMALGNGYPE
                     TGSKNKSVIHWDMICDMRTDSEILVDGELFYKDGQFVFNK"
     gene            5009..>5219
                     /locus_tag="PLR93_03925"
     CDS             5009..>5219
                     /locus_tag="PLR93_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26139.1"
                     /translation="MKQLLQNIKDGKTIVEDVPIPTPREGQALVKVSASLVSAGTERM
                     VVEFAEKSYLGKARSRPDLVKQTLDK"
BASE COUNT         1340 a         1133 c         1471 g         1275 t
ORIGIN      
        1 agaccaaaat ccctgccatc tacgtcaccc acgaccagga agaagccttc accatcgcag
       61 accgcatcct tgttttgcac gaaggcacca tcattcgcga cgctgctccg cacgagattt
      121 ggcgcgagcc caagtctgct tttgtggcga agctgctggg aatcgggaac gttattgatg
      181 gggaagtggt tgcggataaa aaagttaaaa cagcgtttgg cgtttttgca cttgactgta
      241 aaaaacaagt tggagaaaaa gctcaattgc tgattaccat tgaggaagaa aggggagaga
      301 agattcaact cacagtggag gatgtgctat ttaagcagga ccaattccag gtcacggcac
      361 gaggtggaat tactatccac atgaaagaag cgccaaaagt tggcgatatc atcaatatca
      421 aggttcaggt aaaatgcctg tcatgaaaat cctgaaaaag aatctggctg atgaagtgac
      481 ttggcagtat gagggcgagg agttgagtcg tgataagaat tcgatcacga ttgaagcgtt
      541 gtttaatcgt gatgatatgc cgtttcaaga gatcgtacta aaacgcaacg accgctttgt
      601 ggaaactttt tattctgata aatggtacaa catttttgcc atttacgacc gtgatgatgg
      661 caagctgaaa ggctggtact gtaatattac caagcccgct attattgaaa ttgactcggt
      721 ttcatacgtt gaccttgcgc ttgatctttg ggtctctgca aatggaaaac agaccgtttt
      781 ggatgaagat gaattggaag aattgaagtt gggcgaggca ttgaaagaaa atgtatatgc
      841 cgggttgcgt gaattgcaag cgttttttta atcaaaaaaa tcccctgcca tgagcagggg
      901 attttttgtt ggaaattaga aattattcca gacgtagtaa ccgataccgg agccaagcac
      961 acagcagcag cacaacacga caacagcaac gacaccgatg atgatattgc gattgttatt
     1021 gttaggcgct ggggtttcgg atggggcgac gggcatttct tcagacatga tttctctcct
     1081 tttagaaagt tatttttttg gattataaac gatgtcaggc agactgtgcc ttttgtttgc
     1141 ggcttgccca ccaccacagg acaagcacta ggaagataat gaacaaagtt ccagcaatta
     1201 gcggaatgag gatcgctaga atagaagtga tggtggcaat cacatcttca gcgatcgaaa
     1261 ctgggatatt gcccgccccc gctgttgtgg ctgtgaccgc tgggcgcacg gctgctgcct
     1321 ttacggtgtg aacactgcca gctaccagca aaccgcaggc aagtgctagc acggggttga
     1381 catcggtgat gacattggca ctggcggcga aggcgatggc acccgcgacc gggcggatga
     1441 tggtttggat caggtcgttg atgtgattga ccgcgggtac tttatctgcc accatttcaa
     1501 tgatgaccaa cacgccgagt aggatgagga tccacgggtt ggcgagcagg tcgaaagggg
     1561 aggcgaggtt gagggtgttg ggagcgtaat gagcgatgac acccaccacc aaaagcggaa
     1621 tgtaggcatt caacccggcg ctggctgaaa gaccaaaggc agtaaaaatt ccaagtaaaa
     1681 gttccatttc ttctccttat caaagatcgt acaacttgat tatacgattt ttgagtatat
     1741 tcgttgttag cctgatagac cggggaaccc gctccatgtg cctgtgaagt tgaggcgcat
     1801 taggtcaatg ctcaagatga gcaacaatgc cagggtcaag ccggaggcga acgccagcca
     1861 aagctgacgt ggatgttcga aggagttgtc ttgcttcatg atgatgagcc atagcaaccc
     1921 gaagacgatc accaacagcg aaagagtgcc caaggcgctg atataagcaa tggggtacaa
     1981 cacgaaggga ctatcggtaa gaacaagtaa gttgatgccg atgatgcccg ctaccagaat
     2041 cccgagttgt ttccattcga tggcggggcg gtcatccaag gtgcgccaga gggtttgatt
     2101 gataaccggg tagagcatgg cagcgagggc gatgcccatg ccgctgcccg taaagaggcg
     2161 caagacatta ttgggcacat atagatttgg aatttgcgcc agaaaaccag agtttctgcc
     2221 ggtttctttt aacaagtaca ggtaagaatt ggtgccatcc acgccaaagg cgacgaagaa
     2281 accaaagagc accgccatga cgccgcgcga agggagcttg ctgcgttttc cacttacgac
     2341 catttggaaa agcagggtaa gcccggcggc atagaattca ccggtacagc gggcacatag
     2401 ggggagttga cggtcgccaa tgtggaagga acgtacgtca atgcgatgac agacagcata
     2461 gccaatggca tcgagcttac ccaaggcgcc ttcaggcgcg ataataaacc aggcgattac
     2521 ggctgcaatt gcggcaatgg gaacgaccca actggcaata gtttcaagtt ttgagggttg
     2581 tggatttgtt tgcattttga gattataccc agcctgatgt gaaaatctct cttttgagga
     2641 atatttaata ttttgcgtta tcatcttcca tatagaggtg acatcatgaa acgaattgga
     2701 atccttacag gcggcggcga tgcgccgggg ttgaatgcgg tcatccgtgc ggcggtaaag
     2761 acggcgatca atgaatatca gtgcgaggta atcggcatcc gtgatgggta tgacggtttt
     2821 ctcgatacac agaatggaat tgtgcctttg ggcatggaag aggtgcgcgg aatccttccg
     2881 cgtggcggga cgattctcgg cgcggcgaat cgtggtaacc cgtatgctcg caaggtaatt
     2941 agagatggca aagaagtcac gattgatgtg tcggatgaaa tcgtgaaagg cattaaagaa
     3001 ttgggcttgg acgcattgct tgtgcttggt ggagacggca ctttgcatat tgcacatgaa
     3061 ctggctcaaa aaggagtgcc ggtgatcggt gtgcccaaga cgattgataa cgacattggc
     3121 ggaacagaga tcacctttgg gtttgatact gctttgaata tcgctactga agcattggat
     3181 cggttgcata ctacggcaga gtcgcatcac cgcgtgatgg tggtggagtt gatggggcgc
     3241 gatgccgggt ttatcacgtt gaatgcaggt atttcaggcg gggcagatgt gattttgatt
     3301 cctgagatcc cattcaagtt tgagaacatc atgaaaaaga ttcagtctcg cagtgggcag
     3361 ggacggaagt tcagcttgat cgcggtttct gaaggcgcga agcctatcgg tggcatgcaa
     3421 gtctattcgc gcaaagggga tgaggtgtat gtgccgcgct tgggaggaat cgggcaggtg
     3481 gttgccgaat tcattgaaca acaaggcttc gaatcacgcg tgaccgtact tgggcacgtc
     3541 caacgcggtg ggacgcctac cgccttcgac cgtgggcttg cgacccgtta tggcgcggcg
     3601 gctgtccgct tggcggcgag tggcagattc gaccgaatgg tggcattgca atgcggcgag
     3661 atcacaagcc tcccgttggc tgaggcggtt gctgttccca aacgagtgga cccgcaaggg
     3721 gatgccgtca ttacagcaaa aaatatcggc atctcatttg gtgatgaata aaaaaaggtc
     3781 tcgcaaaaag cgagaccttt ttttatttac ttgttgaaca caaactgacc gtctttgtag
     3841 aacaactcgc catctaccag aatttcagag tcggtgcgca tgtcgcagat catatcccag
     3901 tgaatcacgc tcttattctt ggagcctgtt tctgggtagc cattgccaag cgccatgtgg
     3961 aacgagccgc cgattttttc atcgaacaaa atattgccgg tgaatttttg aatgtcgaag
     4021 ttggtgccga tggcaaactc gccgagatag cgtgaacggt catcactatc gagagttttg
     4081 agtaggaagt ctaaattttt ggtggcattg gcttgggtca ctttgccgtt cgagaaggtc
     4141 aactcggcgc cttcgacgct ggtgccttga tagttggcag gataggtgaa cttcacccag
     4201 ccgttgaccg agtcttccac tggaccggta tagatttcgc catcgggcat gttatgcgtg
     4261 ccataggaat tgataaaggt acgacctttg acggagagcg ttaagtccac gttcggtccg
     4321 cgcaggatga cttgattttt gcctttcatg aaatctgctg cggcttgttg tttggcttta
     4381 acgctgttcc aaaacgcaac cgggtctgct tcattggcat ggacggcacc gaaaacaaaa
     4441 tcttcgtact ccatcaaact catgccggca tcttgagcgt aggcttcggt ggggtagagg
     4501 gtggtgcacc atttgaattc gtcggttgcc ccgcggcgga attgaaagtc ggtgataacg
     4561 ccagtagctt tgttacggcg ggcgctcttg gcggggtcga tgtttgtcgt tccctttgtg
     4621 ttggtcgccg aatgaatgcg gatgcgccct tcaaattgtt cataggctac tttataaaat
     4681 gtgggtacga aatccaactg ggcgtcgttg gcataggtca ggtagagttc atcctggctg
     4741 aagggcatgg tgccgggcaa cgccatcatc aaatgcggat gcccgccttt ttccagaatt
     4801 tgaatgtaca gttctcgtaa cagaggttca gcggcggttg tgccttcgag taagatgcgg
     4861 tcaccgggtt ttacacgggc ggaatgttcc accagaattt ttgcgtactt ttgaacgcga
     4921 atgtctgcca agataggctc ctaaatgtca gaatggcgag attatatcac ctccaaaaat
     4981 ttgacccaga ttgggtatac ttttgcccat gaagcaactt ttacaaaaca tcaaagatgg
     5041 caagaccatt gtcgaagacg ttcccattcc cactccgcgt gaagggcagg cgcttgttaa
     5101 agtttcagcc agcctggttt cggcaggcac cgagcgcatg gtggtggagt tcgcagaaaa
     5161 aagttatctc ggcaaggcgc gttcccgccc tgacctcgtc aaacaaacgc tcgataaag
//
LOCUS       DAOQFT010000103         5218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8169_length_5218_cov_8.101879, whole genome shotgun sequence.
ACCESSION   DAOQFT010000103 DAOQFT010000000
VERSION     DAOQFT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8169_length_5218_cov_8.101879"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..605
                     /locus_tag="PLR93_03930"
     CDS             45..605
                     /locus_tag="PLR93_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNH26140.1"
                     /translation="MNSILVEKKFALGQSLQIVQGDITTEEVDAIVNAANEQLAHGGG
                     VAWAISKKGGPKIQAESDAWVRKHGQVSHAHPAWTSGGALPAKYVIHAVGPVWINGRD
                     DEDQKLQAAVTGSLQVADELKCESISMPAISTGIFGFPKDRAAGIIFETIGDYFDKHE
                     GSTVNVVRIVLFDQSTIDAFLKQWAE"
     gene            602..1372
                     /locus_tag="PLR93_03935"
     CDS             602..1372
                     /locus_tag="PLR93_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNH26141.1"
                     /translation="MITSNQNSKIKLVRALLGRAKERREAGAFVAEGVRLVEEAVKAN
                     WTCRFALYDETLSERGKSQVESLRSRGVDVEEVSASVMKSISETEAPQGILAVLEQSQ
                     LPIPDSRNFILIPDQIRDPGNLGTLLRSAAASGVQAVLIPPETTDAFAPKVLRSGMGA
                     HFRLPIHAMTWEEIEKTTNGLQVYVADMDGQSCWDVDLSKPIALIVGNEAEGASESAR
                     KLANGKISIPMSGETESLNAGVAGSVLMFEILRQRSNV"
     gene            1412..2629
                     /locus_tag="PLR93_03940"
     CDS             1412..2629
                     /locus_tag="PLR93_03940"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNH26142.1"
                     /translation="MKIRSITYFCNPGYPLKENILREAGDFLSKAKSAYEAAGYEVQT
                     VRVATVSFAKLLGEKHIDGLPKYAEQFTQVMKQNGVVYAALGPALPELPRSYQVIPDA
                     IKASEGVFFSGVMADAQTIHMAQVKACAEIIVKSAPLDPNGFANLHFTALANVGANAP
                     FFPGAYHTSDQPAFGIAVECADMAVDAFAKAQSIEEGRQWLVSEMTKHGQAITNIANG
                     LKIEFKGIDFSLAPFPDDAHSLGKAVENMGVPKIGLHGSLAAAAILTDAIERADFPHI
                     GFNGFMQPILEDSILAKRAAEGALTVKDALLYSAVCGTGLDTVPLAGDVTADELTPLL
                     LDLCALALRLNKPLTARLMPVPGKKAGDATTFDFAFFANGRVMALESQPLSLPFNSNE
                     SFSLKPRAERNRP"
     gene            2719..>3815
                     /locus_tag="PLR93_03945"
     CDS             2719..>3815
                     /locus_tag="PLR93_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH26143.1"
                     /translation="MKRNILIIGGLVFVAIVAFFVYKAVAPSKPVNVQLLALNDFHGN
                     LLPPSGSSGQVDGVDAGGVEYLATHIDTLRGENPNTVVVSAGDLVGASPLLSALFHDE
                     PTIEAFNLIGLDFNAVGNHEFDEGWAELKRLQEGGCHPVDGCLDGDDFSGAEFKFLAA
                     NVINKETGEPIFPAYEVREFEGVKIAFIGMTLKGTPSIVTPSGVADLEFLDEAETVNA
                     LIPELQAQGIETIVVLVHEGARQNGDAPTANTCQDIDGPIVDIVSRFDDAVDVVISGH
                     THQAYNCLLDGKLLTSAMSFGRLVTQIHLTIDPKTGDVTTMEANNLVVTRDVEKASLL
                     TALIERYNKVAEPLANHEVGTITADILREPN"
     assembly_gap    3816..3915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4121..4678)
                     /locus_tag="PLR93_03950"
     CDS             complement(4121..4678)
                     /locus_tag="PLR93_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH26144.1"
                     /translation="MSEELVAFFKVLSDANRLKIIGLLAHQPYSVEELAALLGLKPST
                     VSHHLSKLSKVGLVSAKTESYYNVYQLDQKALEEKSRSLFSQDKMTASVADVDVDAYD
                     RKVIGDYTRKDGSLKTIPAQRKKLDAVLRHIVKAFKVGKRYSEKQVNEILSTYHDDTA
                     SLRRELVGAKLMQREGGGGEYWRDE"
     gene            complement(4680..5045)
                     /locus_tag="PLR93_03955"
     CDS             complement(4680..5045)
                     /locus_tag="PLR93_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNH26145.1"
                     /translation="MKTRKELNREYAERVKPAGIYQVKNTVSGKMFLGSSLNLEGPLN
                     RHRFMLKIGSHTNKALQNDWNQLGADAFAFEILEEVKMKDDPNFNLHDELTLLEMIWL
                     EKLQPVENGYNLNARIREA"
BASE COUNT         1307 a         1172 c         1312 g         1327 t
ORIGIN      
        1 tcgcgatccg cccctacaat ttttaacggg ggtaaaattg attcatgaat tccatcctag
       61 tagaaaagaa atttgcattg ggtcaatcac tacagattgt gcaaggtgac atcaccacag
      121 aagaagtgga cgccattgtc aatgcggcga atgaacagct cgcccacggc ggcggagtgg
      181 cttgggcaat ttcaaagaag ggtggaccaa aaattcaagc agaaagtgat gcctgggtcc
      241 gcaaacacgg acaggtctca cacgcgcatc ctgcctggac ctcgggcggg gcgctgcctg
      301 cgaagtatgt gatccacgct gtgggtccgg tgtggatcaa tggcagagat gacgaagatc
      361 aaaaattaca agctgctgtg acaggttctt tgcaagtggc agatgaatta aaatgtgagt
      421 cgatctctat gccagcaatt tctacgggca ttttcgggtt tccgaaagac cgagcggctg
      481 gaattatttt cgaaacaata ggggattatt ttgataaaca cgaaggttca actgtaaatg
      541 ttgtgcgtat cgttctattt gaccaatcca caattgatgc ttttctaaaa caatgggctg
      601 aatgatcaca tccaaccaga attcaaaaat aaaacttgtg cgcgccttgt tggggcgtgc
      661 caaagagcgc cgtgaggctg gggcatttgt cgctgaaggg gtgcggctgg tggaagaggc
      721 agtgaaagcg aattggacat gtcgctttgc cttgtatgat gagaccttga gcgaaagagg
      781 caagtctcag gtcgaaagtt taaggtcgcg cggcgtggac gtggaagaag tctcagccag
      841 tgtgatgaag tccattagcg agacggaagc gccgcagggt atccttgctg tgctcgaaca
      901 atcccaactc ccaattcccg attcccgaaa tttcatcctc atcccagacc agatccgcga
      961 cccggggaat ttgggaacgt tgttacggtc tgcagcggct tcgggtgtgc aagccgtttt
     1021 gattccgcct gaaacgacgg atgcttttgc accaaaagtt ctgcgatctg gtatgggagc
     1081 gcatttcagg ctgcccattc atgcaatgac ttgggaagag attgagaaaa ctacaaatgg
     1141 cttgcaagtt tacgtcgccg atatggatgg acaatcttgc tgggatgtgg acttatccaa
     1201 gccgattgct ttgatcgttg gcaacgaagc agaaggggca agcgagtccg cgcggaagtt
     1261 ggcgaatggg aaaattagca ttccaatgtc cggcgagact gagtctctga atgcgggagt
     1321 agcggggtcg gtgttgatgt ttgagatatt aaggcaacgt tccaacgttt gaacgtttat
     1381 aggttttcaa tcttctactg tatgaggaaa aatgaaaata cgatccatta cctatttttg
     1441 taatcctggt tatcccttga aagaaaatat cctgcgtgaa gcgggagatt ttttatctaa
     1501 agccaagtct gcgtatgagg cggctgggta tgaagtacaa accgtgcgtg tggcgacggt
     1561 ttcgtttgcg aaactgctgg gtgaaaaaca tatcgatggt ctgccgaaat atgccgagca
     1621 gtttacacag gtgatgaaac aaaacggcgt cgtgtatgca gcgctgggac ctgctctgcc
     1681 agaattgcca cgcagctatc aagtcattcc agatgcgata aaagcaagtg aaggcgtttt
     1741 cttcagcggc gtaatggcag atgcgcagac gatccacatg gcacaagtca aggcatgtgc
     1801 agagatcatc gtcaagtctg caccgttaga cccgaatgga tttgccaacc tgcatttcac
     1861 ggcactcgca aatgtcggtg cgaatgcgcc attcttccca ggcgcgtatc atacgtctga
     1921 ccaacctgcg tttggaattg ccgtcgaatg tgccgatatg gcagtggatg cctttgcaaa
     1981 ggctcaatcc attgaagaag ggcgtcaatg gctcgtttca gaaatgacca aacatggtca
     2041 agccattacg aacatcgcaa acggtttaaa aatcgaattc aaaggcatcg acttctcgct
     2101 tgcacccttc cctgatgatg cacattcgct gggcaaagcc gtagagaata tgggcgtgcc
     2161 gaagatcggt ttacatggtt cgcttgcagc agcagcaatt ctcaccgatg ccatcgagcg
     2221 agcagacttt ccgcatattg ggttcaatgg tttcatgcaa cccattctcg aagattctat
     2281 tttggcaaag cgtgccgccg aaggtgcgct gaccgtgaag gatgctttgc tttattccgc
     2341 cgtatgcggc acaggtttgg atactgttcc gttggctggg gatgtaacag cggacgaact
     2401 cacgcctctt ctcttggacc tgtgtgcttt ggcgcttcga ttgaacaaac ccctcaccgc
     2461 cagactgatg cccgtcccag gtaaaaaagc gggagatgca accacctttg acttcgcttt
     2521 cttcgccaac ggacgtgtga tggcgttaga gtcacagccg ctgagcttgc ctttcaatag
     2581 caatgagtca ttttcgctga agccaagggc agagagaaac cgtccataaa gaaaaggctg
     2641 aagaggggct gccagttttc ggttaagaat gaagcgggtt ttcatcctgt ttcaaatagt
     2701 tcaagaggaa aataatcaat gaaaagaaat atcctaatta tcggtggatt ggtttttgtc
     2761 gcaatcgttg cgttttttgt atataaagcc gtggcaccat ctaagcctgt aaatgtgcaa
     2821 ttgctggcat tgaatgactt tcatggaaat ttgttgccgc cttctggctc ttcaggtcag
     2881 gtggacggtg tggatgcagg cggcgtagag tatctggcaa cgcatatcga tacgttgcgc
     2941 ggtgaaaacc caaatacggt tgtggtttct gctggtgatc tggtgggagc cagcccgctg
     3001 ctttctgcgc tctttcacga tgaaccgacc attgaagcct ttaatttgat cgggttggat
     3061 ttcaatgccg ttggcaacca tgagtttgac gaaggctggg cggagttaaa acgtttgcaa
     3121 gagggtggtt gtcaccctgt ggatggatgt ttggatggcg atgatttttc aggagccgag
     3181 tttaaattcc ttgcggcgaa tgttatcaac aaagagactg gcgagccgat ctttccagcc
     3241 tatgaggtgc gcgagttcga aggcgtaaaa atcgctttca ttggcatgac gctcaaagga
     3301 acgccaagca tcgtcacgcc ttctggcgtt gccgatttgg aatttctgga tgaggctgaa
     3361 acggtgaatg cgcttatccc tgaattgcaa gcgcaaggga ttgagaccat tgtagttttg
     3421 gtgcatgagg gcgcacgcca aaatggagat gctcccactg caaatacctg tcaggatatt
     3481 gacggaccga tcgtggatat tgtcagccgc tttgatgatg ccgtggatgt agtcatcagc
     3541 ggacatacgc atcaggcata taactgttta ctggatggca aactgcttac tagcgccatg
     3601 tcgtttgggc ggctcgtgac gcaaattcat ttgaccattg accccaagac cggtgatgta
     3661 accaccatgg aagccaataa tttggtcgtg acccgcgatg tggaaaaggc gtcattacta
     3721 actgcattga tcgaacgcta taacaaagtt gcagaaccgc ttgccaatca tgaggtgggc
     3781 actatcaccg cagatattct gcgcgaaccc aatgannnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnacggt caactcgtat ttagcagatg gcggtgaccg cttcaaagtg
     3961 ttgacagaag gaacagatcg cctcgtaggt gtaatggacc ttgatgcttt ggaagcctac
     4021 atgctccaag cttcaccagt agaacccggg gcacaagatc gaattaaggt tattccataa
     4081 acacgaataa aaagagactc tggcaagcca gagtctcttt ttattcgtct cgccaatact
     4141 ctccgccacc gccctcacgc tgcatgagct ttgctcccac cagttcacgg cgtaaagaag
     4201 ctgtgtcatc atgataggtg cttagaatct cattgacctg cttttcgcta taccgtttgc
     4261 caactttgaa agcttttacg atgtgacgca gaaccgcatc caacttctta cgctgagcag
     4321 ggatggtttt caagctacca tctttccgag tgtaatcacc gattacttta cgatcataag
     4381 catccacgtc cacatcggca acagaggcgg tcattttgtc ttgagaaaac aggctgcgag
     4441 atttttcttc cagcgccttc tggtcaagtt gataaacgtt gtaataactt tccgtctttg
     4501 cgctcaccaa gccaaccttt gaaagcttgg acaagtgatg cgagacagta gaaggtttca
     4561 gccccaataa agcagcgagt tcttccacac tatacggctg atgcgcaagc agtccaatga
     4621 tcttgaggcg attcgcatcc gaaaggactt tgaaaaaggc gaccaattct tcgctcatat
     4681 cacgcctccc ggattctggc attcaggttg tagccgtttt caacaggctg aagcttctca
     4741 agccagatca tttccagcag agttaactca tcgtgaaggt taaaattcgg gtcgtctttc
     4801 atctttactt cttcaaggat ttcaaaggca aaggcatctg cacccaattg attccaatca
     4861 ttttgaagag ccttattggt atggcttccg atcttaagca tgaagcgatg gcggttcaac
     4921 ggaccttcaa ggtttaggct gctgccaaga aacatcttac cgcttactgt atttttcacc
     4981 tgataaattc cagccggctt aacacgttct gcatattcgc gattgagttc tttacgggtt
     5041 ttcatttgat cgtccatgtt gagtcatctg tcaacttaag gcgttttcgt gaaagatcaa
     5101 ctctccaata cttcgagcca tcactttcac ggtccataag ccctgcatcc accaaatccc
     5161 gccgcagacc ggcagtatct tcattgaatt gtttgatgat gatgttaact tctcgttc
//
LOCUS       DAOQFT010000104         5213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8188_length_5213_cov_6.517255, whole genome shotgun sequence.
ACCESSION   DAOQFT010000104 DAOQFT010000000
VERSION     DAOQFT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8188_length_5213_cov_6.517255"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PLR93_03960"
     CDS             <1..1406
                     /locus_tag="PLR93_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNH26146.1"
                     /translation="NGRWLTTSDAEVRQNPAIGDITIPDSADRQRLSFTFDVMTSGQT
                     TVYLPPQPIWINHSTILLSSDISNKTGEDAILDIMAVRASPPFQDGDLYRVSSLLADP
                     TAEELRLSGDAYPDWVKEKYLQLPENFSPRIQALAQQITAPYNTPYDKTVAVTNYLRR
                     EIEYTGVIYLPNEDVDPLEYVLFDAKRAFCNYYATAQVLMLRSVGIPARLAVGYAQGQ
                     PNIQKSLYAVREKDLHAWPEVYFPEYGWIEFEPTTNQQPIERPEAREDLAQIAPFANN
                     SNQPLFKPDVEFTPDQEPNVPDGGSASARSWLDWLASLLPWLGGVFFILLGIVLKRRF
                     APQVTVASVMKRAIDRSGWNAPRWLRRWLAFATQPAIQRHFHNINISLRLLKRPQPVH
                     VTAAERAQTLKGLLPSASASIDSLLNELQAELFTPNGGNEAIARRAAWDILSKTVQSK
                     LKIIILGYNYEGIKETS"
     gene            1413..3125
                     /locus_tag="PLR93_03965"
     CDS             1413..3125
                     /locus_tag="PLR93_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH26147.1"
                     /translation="MIENSTRPRTVHDQLSALAVLAGKIRAELGNAKLKLPKESLEQL
                     HYLQSTLETVGNKMEVFQREHGNMLALVETGQVINSSLELDEVLRIVMDNIVRLTKAE
                     RGFLMLRNEGGEMINRVARNWEMESINPSEKNVSRSVVQRVIDTGESVVTTNAQEDQR
                     FVGQESIVAFNLRSILCVPLKVKNDLIGVIYADNRIRAGIFAEAEKDLLEAFANQAAI
                     AIDNARLFSSLKGTLEEVTALKNLMDNVFASIASGVITADVQDTITLANRAAETILGS
                     PTQDIIGHHLKDALASVSGELAPHLMEVRKTDKPIVDLEISHNLPKRGNVDWRLNLSP
                     LKDAGQKTQGVAIVLDDLTERKKLEAQRRLFERMVSPAVIEQLDPNGLQLGGKRTEIT
                     ALFADIRGFTTYSEHLAPERLVSILNLYLAAMADAVLAQEGTVDKFMGDAIMAWFNAP
                     IPQPDHTLRAVKTALAIRESIENLYKELSSDSHLAFGVGIHFGDAVLGLIGTEKRLEY
                     TAISDTVNTAKRLQENSARNQIIISQDAYERVKNDVNVKPISEITVKGRSKPIEVFEV
                     LGLK"
     gene            complement(3174..4262)
                     /locus_tag="PLR93_03970"
     CDS             complement(3174..4262)
                     /locus_tag="PLR93_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH26148.1"
                     /translation="MTLAKKVLDLAIQIQQIASPTFAEGPRGEFIRDLFIQEKLDDVS
                     MDSLGNVYARLPGKQKKARALIVSAHLDTVFPAGTKLQIKEEAGKVFGPGIGDNSIGV
                     AALFGILWGIRERKIELKHDVWFVANVGEEGLGDLRGMRGVVERFGNDELGYLVLEGL
                     ALGHVYHRAIGVRRYRITAKTRGGHSWSDYGQPSAVHELAAMVTQLTQVRLPREPRTT
                     MNVGTIHGGTGVNVLAAEAKCELDLRSEDPVVLAKIVSQVEDIILHWNHEGVKIQAEI
                     IGERPAGQVASDHPLVKLAVESLNAQGMEAILTTGSTDANVPLSKGIPALVMGITTGG
                     GAHTMGEYIDTEPIERGMESLIRFVELS"
     gene            complement(4305..>5213)
                     /locus_tag="PLR93_03975"
     CDS             complement(4305..>5213)
                     /locus_tag="PLR93_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH26149.1"
                     /translation="AMSGLAVWDTSISLIARVNREYPQEWISDFQTRGLDVDGIYVDP
                     ESDRVDIRSFIAYTDTNERSITNAVSHFARREMTFPKSLLGYHPQDPTIKDLRETDPL
                     SPAARFVPKEYRNIGFVHLCPFDFTSQSQLVNLFKGGSNQMVSLDPAPGYMKPKFWRE
                     LRVVLQGVTAFLPSEDELRSLFWGESHDLWEMAQRISEHGPQIIVIKRGPQGQLVYDA
                     ASKRRYEVPAYTARIMDPTGVGDAFCGGFLAGYQRTNDPLQAALHGNVSASLKIEGVG
                     VYATLDAMPGLADARLHALKEMAREI"
BASE COUNT         1395 a         1325 c         1183 g         1310 t
ORIGIN      
        1 aaaacggacg ttggctaaca accagtgatg cagaagttcg tcagaaccct gccattggtg
       61 atattactat ccctgatagc gcagaccgtc agcggctttc tttcaccttt gacgtgatga
      121 cctctgggca gacgactgtt tatcttccac cgcagcctat atggattaat cacagcacga
      181 ttctgctttc ttcggatatt tcgaataaaa ctggagaaga tgccattctt gacattatgg
      241 cagttcgcgc ctctccccca tttcaagatg gggacttgta ccgtgtcagt tccttgcttg
      301 cagacccaac agcagaagaa ctgcgtttaa gtggagatgc atatccggat tgggtcaaag
      361 agaaatatct tcaactgcct gaaaacttct cgccacgcat tcaggcactc gctcagcaaa
      421 tcacagctcc ctataacacc ccttacgaca aaacagtcgc tgttacaaac tatctgcgca
      481 gggaaattga atacactggt gtgatttacc ttcccaatga agatgttgac ccgcttgagt
      541 atgtattgtt tgatgctaag agagctttct gcaattacta tgccacggct caggttctca
      601 tgctacgcag tgttgggatc cctgcccgtt tggcggtggg atatgcccaa gggcagccca
      661 atattcaaaa aagtttatat gcagttcgtg agaaagacct gcacgcttgg cccgaagttt
      721 atttccctga gtatggttgg attgaatttg aacccaccac aaatcaacaa ccgatcgaaa
      781 gaccagaagc tcgcgaagat ttagctcaaa ttgctccatt cgcaaacaac tcaaaccagc
      841 cactatttaa accagatgtg gaattcacgc cagaccaaga accaaatgtg ccagacggag
      901 ggtcagcttc agcgaggtct tggctagatt ggttagcatc gctcttgccg tggttgggag
      961 gcgtgttctt tatcctcctt ggaattgttc tcaaacgacg ctttgctcca caggtgaccg
     1021 ttgcttctgt gatgaaacga gcaatagacc gtagcggctg gaacgcgcca cgttggctgc
     1081 gtcgttggct agcgttcgct acccagcctg cgatccaaag gcattttcat aatatcaata
     1141 tcagccttcg attattgaaa cgcccacagc ccgtgcatgt gactgcggca gaacgggcac
     1201 agaccctaaa ggggttgctg cctagtgcgt ctgcatcaat cgacagcctt ttgaacgagc
     1261 ttcaagctga gttgttcacc cctaacggtg ggaatgaggc aattgcccgc cgtgcagcat
     1321 gggatatcct ttctaaaacc gtacaaagta aactaaaaat tatcatcttg ggatacaatt
     1381 acgagggtat caaggaaacc tcgtaacacc ccatgattga gaactctact cgccctcgca
     1441 ccgttcacga tcaattatct gcacttgccg tgcttgccgg taagattcgc gcggaacttg
     1501 gcaacgcgaa attgaagttg ccgaaggaat cgttagaaca actacattac ttgcagtcca
     1561 ctttggaaac ggttggcaac aaaatggaag ttttccagcg tgagcacggc aatatgttgg
     1621 cgcttgtgga aaccggtcag gtcatcaact catctctaga gttggatgaa gtgctacgca
     1681 tcgtaatgga taatattgtg cgcctcacca aagcggagcg cggttttctc atgttgcgaa
     1741 acgaaggcgg ggaaatgatc aaccgcgtgg ctcgcaactg ggaaatggaa tccattaacc
     1801 cttctgaaaa aaatgtcagc cgttcggttg ttcagcgtgt aattgataca ggcgagtcgg
     1861 ttgtcactac caatgcacaa gaagatcaac gctttgttgg gcaggaaagt atcgttgcct
     1921 tcaatctacg ttcgattttg tgcgtacccc ttaaagtaaa aaatgacctc atcggcgtta
     1981 tttatgccga taacagaatc cgtgcaggga ttttcgcaga agctgaaaaa gatttgttag
     2041 aagcgtttgc caaccaagct gccattgcca ttgacaacgc acgcttgttc tcgtctctca
     2101 aaggtacgct tgaagaagtc acggctttga aaaacctaat ggacaacgtc tttgcttcca
     2161 ttgccagtgg cgtcatcaca gcagatgtgc aagatacgat cactcttgcc aaccgagcag
     2221 cagaaactat cctcggctcc cccactcaag acattattgg tcatcatctt aaagatgcgc
     2281 ttgcttcggt ttcaggtgaa ttggctccgc atttgatgga agttcgcaag accgacaaac
     2341 ctatcgttga ccttgaaatc agtcacaacc tgcccaaacg cgggaatgta gactggcgct
     2401 tgaatctctc tccgctaaaa gatgcgggac agaaaactca aggcgtagca atcgttttag
     2461 acgacctcac agaacgaaag aagttagagg cgcaacgtcg cctgtttgaa cggatggtct
     2521 ctccagccgt catcgaacaa cttgacccga acggtttaca actcggcggt aaacgcactg
     2581 aaatcaccgc tttgtttgca gatattcgtg ggttcacgac ctatagtgaa cacctcgcac
     2641 cagaacgttt ggtgagcatt ctcaatttat atctcgctgc catggcagat gccgttctcg
     2701 cacaagaagg caccgtagac aagttcatgg gtgatgctat tatggcttgg ttcaatgctc
     2761 ccatcccaca gccagaccat acattgcgtg cggtaaaaac tgcgcttgcc attcgcgaat
     2821 cgattgagaa tttgtataaa gaactctcca gtgactcaca tcttgccttc ggcgtaggca
     2881 ttcactttgg agatgcagtt ctgggtttga ttggtacaga aaagcgcctt gagtacacag
     2941 ctatcagcga cacagtcaac actgccaaac gcttacaaga aaattctgcc cgcaaccaaa
     3001 ttatcattag tcaagatgcg tacgagcgtg taaaaaatga tgtaaacgta aaaccgatct
     3061 cagagatcac ggttaaggga cgttcaaaac cgatagaagt ttttgaagtg ctcgggttga
     3121 aataaaacaa aaggcttcca aatggaagcc tttttgattc gaaataatgg cacttaagat
     3181 aattctacaa accgaatcaa actttccatt cctcgctcaa tcggttctgt atcaatatac
     3241 tcgcccatcg tgtgtgcccc accaccagtt gtaattccca tcacaagggc gggaattcct
     3301 ttgctcaatg ggacattggc atctgtagag cctgtagtca gaatagcttc cattccttgt
     3361 gcatttaaac tttctaccgc taacttgacc aaaggatgat ctgaggcaac ttgtcctgca
     3421 ggtcgttctc caataatttc tgcttgaatc ttcacgcctt catgattcca atgcaaaata
     3481 atatcttcca cttgcgagac gatcttcgcc aatacaaccg ggtcttcgga gcgcaaatct
     3541 aattcgcact tggcttctgc agccaacaca ttcacacctg tcccgccgtg aatggtgcca
     3601 acgttcattg tagtgcgtgg ttctcgcggt aaacgaacct gtgttaactg agtgaccatc
     3661 gccgccagtt catgaacagc cgagggttga ccatagtctg accaggaatg tccaccgcgg
     3721 gtctttgctg tgatgcgata gcgcctcact ccgatcgcac ggtgatacac atgcccaagc
     3781 gccaagcctt ccaaaacaag ataaccaagt tcatcgttgc caaagcgctc caccacgcca
     3841 cgcataccgc gcaggtcgcc gagcccctcc tcccccacat tcgccacaaa ccacacatca
     3901 tgcttcagct caatcttgcg ttcgcggatt ccccaaagaa tgccgaacaa cgccgccacc
     3961 ccgatggaat tatccccaat gccgggacca aaaaccttgc ccgcttcttc cttaatctgc
     4021 aacttggttc ccgccgggaa caccgtgtct aagtgggcag atacgatcaa cgcccgagcc
     4081 tttttctgtt tccccggcaa ccgtgcatag acgttcccca acgagtccat ggacacgtcg
     4141 tcaagtttct cttgtatgaa caagtcacgt ataaactcgc cacgcggacc ttccgcaaag
     4201 gtaggagatg caatttgttg aatttgaata gcgaggtcga ggactttttt ggctagtgtc
     4261 atgatggctg caatcgtaaa tcgtcagtca caaatcgtca attgttagat ttctcttgcc
     4321 atttctttca aggcatgcaa acgggcatct gccaaaccgg gcatggcatc tagcgtagcg
     4381 taaacgccaa ccccttctat ttttagtgaa gcagagacat ttccatgcaa agccgcttgc
     4441 aaggggtcgt tcgtgcgctg gtatccagcg aggaatccgc cacagaaagc gtcaccaact
     4501 ccggtcgggt ccatgatgcg ggcagtatag gctggcactt cataacgtct tttcgaggcg
     4561 gcatcatata caagctgacc ttgaggtcca cgcttgataa caatgatctg aggtccatgc
     4621 tcactgatac gttgtgccat ttcccaaagg tcatggcttt ctccccaaaa cagcgagcgc
     4681 aattcatctt cagatggaag gaaggcagtg acgccttgca acacaacgcg tagttctcgc
     4741 caaaattttg gtttcatata accgggggca gggtctaaac taaccatctg atttgagccg
     4801 cccttgaaaa gattaaccaa ttggctctgg ctggtaaagt cgaaaggaca aagatgtaca
     4861 aagccgatat tgcgatattc cttcggaaca aatcgtgctg caggagaaag tggatcggtc
     4921 tcgcgtaagt cttttatagt tgggtcttga gggtgatacc caagcaggga tttgggaaag
     4981 gtcatttcgc gacgagcaaa atgtgaaacg gcattggtaa tgctgcgttc attggtatcg
     5041 gtatatgcaa taaaactacg gatatcaaca cggtcgcttt cagggtctac ataaatacca
     5101 tcaacatcaa gacctcgagt ttgaaaatca cttatccatt cttgcggata ttctcgattt
     5161 acccttgcaa ttaatgaaat agatgtatcc catacagcca agccgctcat ggc
//
LOCUS       DAOQFT010000105         5195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8249_length_5195_cov_6.103113, whole genome shotgun sequence.
ACCESSION   DAOQFT010000105 DAOQFT010000000
VERSION     DAOQFT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8249_length_5195_cov_6.103113"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLR93_03980"
     CDS             complement(<1..826)
                     /locus_tag="PLR93_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH26150.1"
                     /translation="MSPESSPSKTKVCPTCGTRLSENAVRCLVCGTEFGAQPAPKAVK
                     KTEKSVQAASMPQLTLSLPAAIGSLLAVIAVAAGLTFFLVPKDPETFSNVETSTPTET
                     PTATIPATATLPATETPTPTLQPPFEYTVSAADGSCSQIAFNFNVSVQSIIITNNLPS
                     SCPISVGQKLMIPYPTPTVPPPATNTPPPDVATQQACETVQITVQENDTLSSIAANYA
                     VSMDAIKEFNGLTTDNVFLGQVVQIPLCERAPDPNKPTPTATLPPPYPAPNLLLPAD"
     gene            complement(890..2182)
                     /locus_tag="PLR93_03985"
     CDS             complement(890..2182)
                     /locus_tag="PLR93_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNH26151.1"
                     /translation="MSISSEIITIIILIVINGVLAMSEAALVASRKARLQQQASEGNK
                     ASALAVKLIEDPNIFLSAVQIGITLIGVLSGAVGGATIAEKLAVFFEKIPYIGPYSAS
                     VSLGLVVLVITVLTIWLGELVPKRLGINSPERIAQIVAGPMLFISRVFSPLIKLMSNA
                     TNFVLRIFGVNPSVEPPITEEELQLLIDQGTQAGVFEEAEQDMVEGIFSLGDTRVYSV
                     MTPRTEIVWLDVSDSTEEILEKMSGSPYSRFPVRQDSLETIVGIVKSRDLLVSILSQK
                     QINLKELAKPAHFIPETMLASRALEVLKKNNAEMLLVVDEFGGVQGLLTINDILEEIV
                     GVMEGDEPQATQRQDGSWLLDGMLEVDEFKEIFNLNELPHETEYETLSGFIMTSLGRL
                     PQTADHFEWNNLRFEVVDMDGRRVDKVLVTAKQKTGTD"
     gene            complement(2374..2520)
                     /locus_tag="PLR93_03990"
     CDS             complement(2374..2520)
                     /locus_tag="PLR93_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26152.1"
                     /translation="MFRVGVLELGVTCAFVALTIIIPLLIARGYAQINKRLKNIENTI
                     AKKK"
     gene            complement(2522..3580)
                     /gene="selD"
                     /locus_tag="PLR93_03995"
     CDS             complement(2522..3580)
                     /gene="selD"
                     /locus_tag="PLR93_03995"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3518..3520),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNH26153.1"
                     /translation="MSIPQSGREVRLTTLSACAGUASKLSADALAQVLRPVKDIFDPA
                     AYPDLMVGLESPDDAAIWRLSDDQAIVVTTDFFTPVVDTPYEYGAIAAANSLSDVYAM
                     GGRPFLALNIAALPDHLPAEISGEILRGGAEKAREAGVVIAGGHTVKDKEPKYGLVVI
                     GFVHPQKVISKGGLKAGDVLVLTKPLGAGVTTTALKQEKADEAHVLEAIEWMASLNKN
                     AGMLASEFGVRGGTDITGFGFLGHASEMAEASHVTLHFDYSKIPFLSGARGYAERGIF
                     PGGAFDNKSHFEALVKFSAEISEPAQMLLFDPQTSGGLLFGLAKEKLSAFEARAKEIN
                     QPYWVIGEAREGEGIEVG"
     gene            complement(3638..4651)
                     /locus_tag="PLR93_04000"
     CDS             complement(3638..4651)
                     /locus_tag="PLR93_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNH26154.1"
                     /translation="MNISSLLQGIASFAWIGFIGILVTISVRASRKQPVGGLSTAAII
                     LVVTAVLLTTVGAGIVFIEPDELGIVVTVLGSGGIRPDPLDSGLHWIVPFAERVERYP
                     KTNQTYTMTSVADEGQVAGDDSIQVRTKDGQQVYIDASVIYAVDPIKVVQLYSTWRFS
                     YEDQFVRTQARGVIRDVASQYGVEEIVSSKRAEMEQSIDDQLVAIFAENNLIMRDFVL
                     RNIRFSDEYAAAVEQKQIAEQQAQQAKFVVEQKKQEAEQARQVAQGSADAAVIAAKGE
                     AEALLIQAQAQAEANQVIAQSLTPELVQYQYVQKLSPNVQTIFIPSGNQFILPLPSSP
                     QGQ"
     gene            4724..>5195
                     /locus_tag="PLR93_04005"
     CDS             4724..>5195
                     /locus_tag="PLR93_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26155.1"
                     /translation="MKTGTGKIEELFLDGSARISCPPELIPAPGQYLLAHAAGSDAPL
                     PVPLFSSLSSSQNGFRFAPPIPSSWQPGQLLHLRGPIGHGFSLPSSARKIALVGLEDT
                     PARLLGLLSQALKQSAEVVLVSNSSVEDLPEVVEIQPVKGLLDVLRWADFVAMDV"
BASE COUNT         1287 a         1314 c         1252 g         1342 t
ORIGIN      
        1 cgtccgcagg caggagcagg ttcggggcgg ggtaaggagg cggaagcgtc gcagtggggg
       61 taggtttatt gggatccggt gcgcgttcgc agagcggaat ctgaactact tgcccaagga
      121 agacgttatc ggttgttaga ccgttgaact ctttgatggc atccatggaa acggcataat
      181 ttgcagcgat gctagaaaga gtatcgtttt cttggacggt gatttgtacg gtttcgcagg
      241 cttgttgggt tgcaacatct ggcggcggtg tgttggtggc tgggggcgga accgttgggg
      301 tggggtacgg aatcatcaat ttttgcccca ccgaaattgg acaactggaa ggtaagttgt
      361 tggtaatgat aatgctttgt acagatacgt tgaagttgaa cgcgatctgt gaacaagaac
      421 catctgctgc cgaaaccgtg tattcaaagg gaggctgcag agtgggcgtg ggtgtttctg
      481 tagcaggtaa ggttgcggtt gcagggatgg ttgcggtggg ggtttctgtg ggtgtggagg
      541 tctctacatt ggaaaaagtt tccgggtctt taggaacgag gaagaatgta agacctgctg
      601 caactgcgat cacagccaga agtgagccaa tggctgcggg aaggctgagc gtaagctgtg
      661 gcatgctggc agcttgaacg gatttttccg ttttcttcac tgctttgggt gcaggctgtg
      721 cgccaaattc cgttccgcat accaaacagc ggacggcatt ctcacttagg cgtgttccac
      781 aggtaggaca gacctttgtt ttgctgggag atgattcagg actcatacgg gcgaaaatta
      841 taccatcgaa caaatctacg atttttaggc gatttagcta cgaacgggct tagtctgtgc
      901 ctgttttttg ctttgccgtg actaaaactt tatcgacacg gcgcccatcc atatctacca
      961 cttcaaagcg gaggttgttc cactcgaagt ggtctgcggt ttgtggcagc cgaccgagcg
     1021 aagtcataat gaatccgctc agggtttcat actcggtttc gtgcggtaat tcgtttagat
     1081 tgaaaatctc tttgaattcg tccacttcaa gcatgccatc taataaccac gaaccatctt
     1141 gacgttgtgt ggcttgcggt tcgtcacctt ccatcacgcc gacaatttct tcgaggatgt
     1201 cattgatggt cagcaaacct tgtacgccgc caaattcatc cacgacgagt agcatttcag
     1261 cgttattctt ttttagcacc tctaaggcac gtgaagccaa catggtttca gggatgaagt
     1321 gcgcaggctt ggcaagttct ttcaggttga tctgcttttg agaaagaatg gacaccaaca
     1381 aatctcgtga tttaacaatg ccaacaatcg tctctaatga gtcttggcgt accggaaagc
     1441 gtgagtatgg actgccgctc atcttttcga gaatctcttc cgtggagtcg cttacatcca
     1501 accaaacgat ttcagtacgt ggagtcatca ccgaatagac gcgggtatcg ccaaggctga
     1561 aaatgccttc gaccatatct tgttccgcct cttcaaaaac gccggcttgt gtgccttggt
     1621 caatcagcaa ttgaagttct tcttctgtaa tgggtggttc gaccgaagga ttcacgccaa
     1681 atatccgcaa gacaaaattc gtagcattgc tcattaattt gatcaatggt gagaacaccc
     1741 gcgaaatgaa gagcatcggt ccagccacga tctgagcgat ccgttctggg ctgttaatgc
     1801 caagtcgctt cggaacgagt tcgcccagcc aaatggtgag cacggtgata acaagtacca
     1861 caagccctaa tgagactgat gcgctatagg gtccgatata tggaattttc tcaaagaaga
     1921 cagccaactt ctcggcaatg gtcgcaccac cgaccgcacc agaaagcacg ccgatcaagg
     1981 tgatgccaat ttgcactgcc gataaaaata tattggggtc ttctattaac ttaacagcca
     2041 atgccgaagc tttattgcct tcacttgcct gttgttgcaa gcgcgccttg cgtgaggcaa
     2101 ccaatgccgc ttcagacatt gctaaaacgc cattgatgac gatcaagatt atgatggtaa
     2161 tgatttcaga actaatgctc attgcttcct atgggactct agaatttcgt ttgatactgc
     2221 ttaaaaagat acaagccgcg cataaagaac ttcgcggcgt ggagatttcc accaagctaa
     2281 ttgaaacgga cagggggaac tacggtctgg ttctatggga atgtcggaca tgatgtttcg
     2341 tgctaagtaa gtcgccctta ttgtacctga aagttacttc ttttttgcga tagtattttc
     2401 aatattcttc aaacgtttgt tgatttgtgc atatccacgg gcaatcaata acggaataat
     2461 gattgtcaac gctacaaatg cacaagtgac acccaactcc agcacgccaa ctcggaacat
     2521 actaaccgac ctcgatgcct tcgccttcgc gtgcttcgcc aattacccaa tacggttggt
     2581 ttatttcctt tgcccgcgct tcaaaagcgg acagtttttc ttttgccaaa ccaaacaata
     2641 atccgccgct ggtttgcggg tcgaacaaaa gcatttgagc aggttcgctt atctcagcag
     2701 agaacttcac cagagcttca aaatgacttt tgttatcaaa cgctccacca gggaaaattc
     2761 cacgctcagc ataaccacgt gcgccactca aaaatgggat tttcgaataa tcaaaatgta
     2821 atgtcacatg agacgcttcc gccatctctg aggcatgacc cagaaaaccg aatccggtga
     2881 tatccgtccc gccgcgaacg ccaaattcag aagcaagcat ccccgcattt ttgttcaaac
     2941 tcgccatcca ttcaatggct tcgagaacat gcgcctcatc agctttttct tgttttagcg
     3001 ccgtcgtggt gactccagcg ccgagcggtt tggtaagcac aagtacatcg ccggctttca
     3061 agccaccctt gctaatcact ttttgtggat gcacaaaccc gatcaccacc aagccgtatt
     3121 ttggttcttt atctttgacc gtatgcccac ctgcaataac gactcccgct tcacgggctt
     3181 tctcggcacc accacgcaaa atttcgcctg aaatctccgc tggtaggtga tctggcaatg
     3241 ccgcaatatt caacgccaag aacggacgtc cgcccatggc atagacatct gagaggctat
     3301 ttgctgcggc aatcgcgccg tattcataag gagtgtctac aacgggagtg aagaaatctg
     3361 tcgttaccac aatcgcttga tcgtcactta aacgccaaat tgccgcatca tccggcgact
     3421 caagacccac catcaagtcg ggatatgcag cagggtcaaa gatatctttg acagggcgca
     3481 gaacctgcgc cagcgcatca gcgcttagtt tagacgctca accagcacaa gctgaaagag
     3541 tggtgaggcg cacctcacgt ccgctttgtg gaatggacat atgctccttt atagattaca
     3601 gacctgacgg gaaccgtcag gtctgtagtg tactcgatta ctgcccttgg ggcgaactgg
     3661 gcaacggcag aataaattga ttgccgcttg ggatgaagat cgtctgaacg ttcggcgaaa
     3721 gcttctgcac gtactgatat tgaaccagtt ccggcgtgag tgattgagcg atgacttggt
     3781 tcgcctctgc ctgcgcctga gcctgaatca acaacgcctc agcctcacct ttcgctgcga
     3841 tcacggcggc atctgcagaa ccttgcgcca cctgacgagc ctgttcagct tcctgcttct
     3901 tctgctccac tacaaatttt gcttgttgtg cctgttgctc agcgatttgc ttctgctcaa
     3961 ccgctgcggc gtattcatca ctgaagcgga tgttgcgcaa aacaaaatcg cgcataatca
     4021 ggttgttttc agcaaagata gccactaatt gatcatcaat cgattgttcc atttcagcgc
     4081 gcttcgagct gacgatttct tctacgccat attgtgatgc cacatcacgg atgacgccac
     4141 gtgcctgagt gcgcacaaat tggtcttcat agctaaaccg ccaagtggaa tacaactgca
     4201 ccactttgat cgggtctaca gcgtaaatga cggaagcgtc aatataaacc tgctgaccat
     4261 ctttcgtgcg gacctgaata gagtcatccc cggcaacttg cccctcatcg gctactgacg
     4321 tcattgtata cgtctggttt gttttggggt accgctctac gcgttcagca aaaggcacaa
     4381 tccaatgcaa ccccgaatca agcggatctg ggcgaatacc gccagagcct agcaccgtca
     4441 caacgatacc gagttcatct ggttcaatga acacaattcc cgcacccaca gtggtcaaca
     4501 aaaccgctgt aaccaccaaa ataatcgcag ccgtgcttaa cccaccaacc ggctgttttc
     4561 tactagcgcg cactgagatc gtaaccaaaa ttccaataaa accgatccat gcgaaagaag
     4621 caataccttg cagcaaactt gatatattca tggttcctcc aattgatctc gcaaaattat
     4681 aactggcttg tcatggctac cgaagcatgt taatattttt caaatgaaaa ctggcacagg
     4741 aaagatcgaa gaactttttt tggatggctc cgcccggatc tcatgtccgc ccgagctgat
     4801 tcctgcccct gggcagtacc ttctcgccca tgcagccggc tcagatgcac ccttaccggt
     4861 tcctcttttc tcaagccttt cctcatctca aaacggattc cgcttcgcgc cgccaatacc
     4921 ttcttcctgg cagccaggtc agttactaca tctacgcgga ccgatcgggc atggtttcag
     4981 cctgccgtct tcggcaagga aaatagcgct tgttggcttg gaggataccc ctgcacgatt
     5041 gctgggactg ctttcgcaag cgttgaaaca aagcgccgaa gttgtattgg tctccaactc
     5101 gtctgttgaa gatttacccg aggtggtaga aattcagcct gttaaagggc tccttgacgt
     5161 gcttcgctgg gcagactttg ttgccatgga tgtaa
//
LOCUS       DAOQFT010000106         5171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8305_length_5171_cov_5.407936, whole genome shotgun sequence.
ACCESSION   DAOQFT010000106 DAOQFT010000000
VERSION     DAOQFT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8305_length_5171_cov_5.407936"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLR93_04010"
     CDS             <1..845
                     /locus_tag="PLR93_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26156.1"
                     /translation="HKLKVGESGIVGYVAKTGVPRIALDVGQDAVFFNNPDLPNTRSE
                     VALPLKVRENIIGVLDVQSERPGAFSEDDTNIFNILADQVAITLENTRLFEQTQQALD
                     EVRSAYQRNLQEGWKVFGQEEGIIGYQQSITGGKKLAKPVHLDEIQQAMHRGQVQEFH
                     PDGKTGEPTLVVPIKLRDQIIGVMHIKAPSKDRLWTQDETNLVEAVSERLSLALENAR
                     LIQESQRQVIKEQTITEITSRIGSSINLDNVLLTAVEELGRNIPGSEVTIKLKNENKN
                     DGSE"
     gene            861..3329
                     /locus_tag="PLR93_04015"
     CDS             861..3329
                     /locus_tag="PLR93_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNH26157.1"
                     /translation="MKQRFSIQDLLARTPLGNLNLRGKLTAGNMIITLLVIAVMGIYV
                     YFRIQTASQQLITSVDENARNRAEEDLVNTNREQATFLDSFFVNMTNNTSIATAAIRN
                     ILDDSQLANSTYWDANTQLIRLENGSFDNQNAEITSIFIPTNVNLNNALAIKLNLLKH
                     SELIFPPMLKGNPDIVAIYFGGESKETIYYPNIDLANIVPADFDVTGRGWYLAAKPSN
                     NPKGGVVWSAPYEDAALNGLVITTSAPVVDKIGRFQGVTAIDVKITRIIDLVANVKIG
                     DTGYAFLIDDSNRLISLPETGYNDFNITDENAKLSIIMDPAALSSVTPEFTDVMEKIK
                     SQEQGIFSIVLNGAEKHITFHNIPEVNYKLVYVIPSEELFSSRQLISEQISNETRSTI
                     NFSVLLIAIVFLVAVGTSFAINNSFTAPLQPLNEAASEIIKGNFDASVEVKSRDELET
                     LATTLNTMTDTVKDLVASLEKRVYERTIELQQANQEGEKRGKQYEAIAKVAQAISTQK
                     NLQELLPQITNVISEQFGFYHVGIFLNDALNKYAVLVAANSAGGKRMLKRGHQLKVGA
                     QGIVGYTTGMKRPRIALNVGDDSVFFNNPDLPDTRSEVALPLMETGNILGALDVQSTE
                     PNAFSEKDLEVLSVLAELVSIAIENAKLYEKMDRSLAEAEAASRQFFRENWNRLAEEY
                     RIAGYRYTAGGATSLSIAQEDVASSIPSDRRQVQVPIIMRGQEIGELSVVIPKDENIK
                     ADQMDLIKAVADRVAVIAENARLFDETTRRAERERLVTDISTKIRSTNDPQAMIETAI
                     NELREALKVSRIEIVPQKKNITDR"
     gene            3358..4143
                     /locus_tag="PLR93_04020"
     CDS             3358..4143
                     /locus_tag="PLR93_04020"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNH26158.1"
                     /translation="MTKTIAVSNEKGGVAKTTTVLSLGAALAELGNRVLLIDLDAQAN
                     LTLALGLEPGETDLTSSNVMLDNEPILKVCRKTEIPDLDLIPSSSRIETSEQFLPMRT
                     NYTTILQRAIQSAPLPYDYIVLDCPPALGAITRNALAAANLLLIPTQAEYFSAYALRN
                     MMGLIRRTREMDNPNLSYRILVTMLDRRNRTHRNIHEQLRATFGSGVFNTVIELDTKL
                     RESPIAGQPITHYKSAARGSTQYRVLAQELMEYAKETENHQAS"
     gene            4109..>5171
                     /locus_tag="PLR93_04025"
     CDS             4109..>5171
                     /locus_tag="PLR93_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26159.1"
                     /translation="MPKKPKITKRLDDLFKDVKPEENAKGRATKPKSQKEAPPPLPAD
                     PKPAQPAQAASTPVKTTRRQTGTLALPSKFIGRDTNAEAKSSYATTIQLGEEEWATLR
                     LVDNQSERSFSTDEQLLIRQVTDQLSLALENARLFQETQRRAQELEIVNKVVSAASGS
                     LDLQSTLQSVADNLVSLLDAQNVGIALINDEKTHLVLAAEAFSTLESSTGIGLLIPIE
                     GNLSTEEVLRTRKPLFIRDVDNNPLLVDSVREALQQRSTKNLLILPLNSEQEVIGTIG
                     VAFFDANRVIAQDELRILETIVAQIAAAVQKNRLFTQVEQSETNFRALFSAMEDVVLV
                     INREGRYMQIAPTNPSLLVR"
BASE COUNT         1537 a         1228 c         1121 g         1285 t
ORIGIN      
        1 gacacaagct caaagtcggt gagagcggta tcgtggggta tgtcgctaaa actggcgttc
       61 cacgcatcgc cctagatgta ggtcaagatg ctgtcttctt caacaatcca gatcttccta
      121 atacacgttc tgaagtggcg cttcctctca aggtgcgtga aaatattatt ggtgttctag
      181 atgtgcaaag cgaaagacct ggcgcattca gcgaagatga cacgaatatt ttcaacattc
      241 tggcagacca agtggcaatt acacttgaaa acacacgcct ctttgaacaa actcagcaag
      301 cactcgacga agtgcgatcc gcatatcaac gcaaccttca agaaggttgg aaagtttttg
      361 gtcaagagga aggcattatt ggatatcaac aatccataac cggaggcaaa aaattagcca
      421 agccggttca cttggatgaa atccaacaag ccatgcatcg cggacaggtc caagagtttc
      481 atcctgatgg gaaaacaggc gaacctacct tggttgtgcc tatcaagctt cgtgaccaaa
      541 tcattggcgt gatgcatatc aaagcgccat caaaagatcg tttatggacg caagatgaaa
      601 ccaatcttgt tgaagctgtt tccgaacgtc tctcgcttgc gcttgaaaat gcacgcctga
      661 ttcaagaatc acaacgtcag gtcattaaag agcaaacaat cactgaaatt acgagccgta
      721 ttggttcatc catcaattta gataatgtac ttctaaccgc ggtggaagaa ttaggacgta
      781 acattcccgg ctcagaggta accatcaaat tgaaaaacga aaacaagaat gatggcagtg
      841 aataaggaaa aaaacaagcc atgaagcaaa gattttcaat tcaggacttg cttgctagaa
      901 ctccgcttgg caacctcaat ttacgaggaa agctaactgc gggaaatatg attattaccc
      961 tccttgtcat tgcggttatg ggtatatatg tatattttcg tatccagaca gccagtcagc
     1021 aattgatcac cagtgtggat gaaaatgcgc gcaaccgtgc agaggaagat ttagtcaata
     1081 ccaaccgcga acaggcaacc ttccttgata gcttcttcgt caacatgaca aacaatacat
     1141 ccattgctac ggctgcaatt agaaatatat tggatgattc tcaattagca aatagcacct
     1201 actgggatgc aaacactcag ttgatacgtt tggaaaacgg cagttttgac aatcaaaatg
     1261 cagaaataac ttctatattt attcctacca acgttaactt aaacaatgca ctggcaatca
     1321 aattaaatct tctaaagcat tcagaattga ttttcccgcc catgttgaag ggcaatcctg
     1381 atattgttgc tatctatttt ggtggtgaaa gtaaagaaac catctattat cctaatattg
     1441 acctggcaaa tatcgtccct gccgatttcg atgtgaccgg acgcggttgg tatcttgccg
     1501 ccaagccaag taacaacccc aaaggcggag ttgtttggtc tgccccatat gaagatgccg
     1561 cactaaatgg attggtcatt actactagtg cgcctgtggt cgataaaatc ggtaggtttc
     1621 aaggcgtaac tgccattgat gtaaaaatca ctcgtattat tgaccttgtt gccaatgtga
     1681 agatcggtga cacagggtat gcattcttaa ttgatgatag caatcgcctc atttctctac
     1741 ccgaaactgg ctacaatgac ttcaacatta ctgacgaaaa tgccaaactc agcattatta
     1801 tggaccccgc tgctctatct tctgtgactc cagaatttac tgatgtcatg gaaaagatca
     1861 aatcgcaaga gcagggcatt ttctccattg tgctgaatgg agccgagaaa cacatcactt
     1921 tccataatat cccggaagtc aattacaaac tggtttacgt cattccgtct gaggaactgt
     1981 tctcaagtcg tcaactcatt tctgaacaga tatcgaatga aactcgttca acgatcaatt
     2041 ttagtgtttt gttgattgcc atcgttttct tggtcgctgt cggcacatcg ttcgccatca
     2101 ataatagttt tacagcgccg ttacagccac taaacgaagc tgccagtgag attatcaaag
     2161 ggaactttga tgccagcgta gaagtaaaat cacgggatga gttggaaacc ctagccacaa
     2221 ccctcaacac catgaccgat accgtaaagg acttggtcgc ttctcttgaa aaaagagtgt
     2281 atgagagaac catagaactt cagcaagcca atcaagaagg cgaaaaacgt gggaagcaat
     2341 atgaagctat cgctaaagtt gcgcaggcga tcagtaccca aaagaacctg caggaattat
     2401 tgccgcaaat taccaacgtg atcagcgagc aatttggttt ttaccatgtg ggcatcttct
     2461 tgaatgatgc acttaataaa tatgccgttt tagtggcagc caacagtgct ggcggaaaac
     2521 gcatgttgaa acgcggacat caattgaaag ttggcgcaca aggcattgta gggtacacca
     2581 cagggatgaa aagacctcgt attgccctta acgtaggtga tgactcggtc ttcttcaata
     2641 acccagacct tcctgataca cgctctgaag tggcattgcc gcttatggaa actggcaata
     2701 tcctcggcgc gctagatgtg caaagcacag agccgaacgc cttctctgaa aaagacctcg
     2761 aagtgcttag tgttctggca gaattggtaa gtatcgccat tgaaaacgca aaactctatg
     2821 aaaaaatgga ccgatctctt gcagaagcag aagcggcttc acgccagttc ttccgcgaaa
     2881 attggaatcg ccttgcagaa gagtatcgta ttgcaggtta tagatatact gcaggtggag
     2941 ccacatccct ctctatcgct caagaggatg tcgccagttc cataccatct gaccgaagac
     3001 aggttcaagt ccccatcatc atgcgtggac aagaaatagg cgagctttct gtggtcattc
     3061 caaaagatga gaacatcaaa gctgaccaaa tggacctcat caaagctgta gcagaccgtg
     3121 tagccgtgat cgcagaaaat gcccgcttgt tcgatgaaac cacacgtcgc gcagaacgcg
     3181 aacgcctggt aacagatatt tcaacaaaga tccgcagtac gaacgatccg caagccatga
     3241 tcgaaactgc catcaatgaa ttacgtgagg cgctgaaggt ctcacgtatt gagattgttc
     3301 cgcaaaagaa aaatattaca gaccgttagc ggtgttttag gaaatgagga atacaacatg
     3361 acgaagacca tagccgtatc taatgaaaaa ggaggagttg ccaaaacaac aaccgtgctc
     3421 tcccttggtg cagcattggc agaacttggc aaccgcgtcc ttctcatcga tttggatgcg
     3481 caagcaaacc tcactctggc tcttggttta gagcccggag agaccgatct cacctcaagc
     3541 aacgtcatgc ttgataacga acctattctc aaggtctgtc gaaaaacaga aattccagac
     3601 ctcgatctta tcccgtccag ttcacgcatt gagacctcag aacaattttt gccaatgcgc
     3661 acaaattaca ccaccattct tcaacgtgct attcagtcgg caccattgcc ttatgattac
     3721 atcgtgttag attgtccacc cgcattgggc gccatcacac gcaatgcgct ggctgctgcc
     3781 aatttattgc ttatccctac ccaagcagaa tatttctctg cttatgcttt gcgcaatatg
     3841 atggggctta tccgccgcac gcgcgaaatg gataacccca atctctcata tcgtatcctt
     3901 gttaccatgc tagaccgacg taaccgcacg catcgcaaca tccatgaaca actccgtgcc
     3961 acatttggtt cgggggtctt caacaccgtt atcgagttag atactaaact tcgagaaagc
     4021 ccaatagctg gacagcctat tacacattac aagtctgctg cccgtggttc tacccaatat
     4081 cgtgtcctcg cccaggaatt aatggaatat gccaaagaaa ccgaaaatca ccaagcgtct
     4141 tgatgatctc ttcaaggatg taaaacctga agagaatgcc aagggacggg caaccaagcc
     4201 caagtcacag aaagaagcgc caccgcctct tcctgcggac ccgaagcctg cgcaacccgc
     4261 ccaagctgca tctacaccgg taaagacaac ccgtcgccaa acgggtacgc ttgctttgcc
     4321 ttcaaaattc attgggcgtg atacaaatgc cgaagcgaag tcttcgtatg ctacaacgat
     4381 tcaacttgga gaagaagaat gggcaactct tcgccttgta gataatcagt ctgaacgctc
     4441 attttcaact gatgagcaat tgcttattcg gcaagtgact gaccagttat cgctggcgtt
     4501 ggaaaatgca cgtctgttcc aagagacaca gcgccgcgca caagagcttg aaatcgttaa
     4561 caaggttgtg tctgcggcat ctggttcgct tgacctgcaa agtaccttgc aaagtgttgc
     4621 cgataacctt gtaagcttgt tggatgcaca aaatgttggt attgcgctaa tcaacgatga
     4681 aaagacacat cttgtccttg cggcagaagc attctcaacc ctagagagtt caactggaat
     4741 tggactgtta attccaattg agggcaatct cagcacagaa gaagtcttac gcactcgtaa
     4801 accattattt atcagggacg tagataacaa tccgctccta gtggactctg ttcgagaagc
     4861 acttcaacaa cggagcacca aaaatttact gatcttgccc ttgaattctg agcaagaagt
     4921 gataggcacc attggcgttg catttttcga tgccaaccgt gttattgctc aagatgaact
     4981 tcgcattctt gagaccattg ttgcccagat cgctgcggct gtacaaaaga acaggttgtt
     5041 tactcaagta gagcaatctg aaacgaactt ccgcgcgcta ttctcggcaa tggaagatgt
     5101 ggtgttggtt atcaaccgtg aagggcgtta tatgcaaatt gcccctacca acccatctct
     5161 cttggttcgc a
//
LOCUS       DAOQFT010000107         5150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8354_length_5150_cov_5.321099, whole genome shotgun sequence.
ACCESSION   DAOQFT010000107 DAOQFT010000000
VERSION     DAOQFT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8354_length_5150_cov_5.321099"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLR93_04030"
     CDS             complement(<1..741)
                     /locus_tag="PLR93_04030"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNH26160.1"
                     /translation="MKIGIIGALDEELIPIRALSSHLEKIERGNRTYWHGDMHGHQVY
                     LTRCDPGKVNAVIATQQLLDFFEPDVLFNMGSSGALAPNLEVGDLVIATDAIQHDFDL
                     TGWGMKPGEILFDVVTKPETGQLGFTSRHAFATDPKLTELAFQSAQSVSLDEIGGHAP
                     KVYKGRVVSGDQFISSVEKAGQLLSTHNALAVDMEAAAIAHTCEINKVPFLCVRAMSD
                     KADHSANVSFTDFLVAATDNYGKVFDKLI"
     gene            complement(771..1601)
                     /locus_tag="PLR93_04035"
     CDS             complement(771..1601)
                     /locus_tag="PLR93_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26161.1"
                     /translation="MDYLKFRSLWGVEEPLESVLPKIKALGYAGVECPLPEPEKSKSF
                     KSLLSETGLKYIPLIFTSGNTVNDHIQTFQAQIDLALSFDPYLINAHSGRDSWTPIER
                     NQFFTEALKFEQGIKARVSHETHRGRILFNPWVTQDVMKDFPDINLCCDFSHWVCVSE
                     RLLLNEEEAYIKTAAEHCLHIHARVGYEEGPQVPDPRALEYQTHLDAHERWWQMVWDA
                     QSRRGFSATTLTAEYGPPLYLHTIPHTNQPVADLWEVCEWQAQHTAEHYANWKAKLVK
                     "
     gene            1775..2587
                     /locus_tag="PLR93_04040"
     CDS             1775..2587
                     /locus_tag="PLR93_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26162.1"
                     /translation="MKKINADLLSLIVIAAFSFMFATAIHEHGGHALACVLQGGHISE
                     MGAFYVDCTRTSLSEFGNRVVAVAGPLVSFLTGLIALGYFYRNQRTNSSLTFFLWHFA
                     TVNLMMASGYFLFSGVAGIGDLGTGESGAFQGVQPEWLIRMGLSVLGLVFYYAVIRIS
                     IKMMDHFIGGEGRERVGRAQMLSVIAYLVGGGIAILIGLFNPYGIVIVLVSSFASSMG
                     GTSGMAWMMQLLDRSKDTGMPAFAMPRNWSWIFVGVGFILLYAIIFGRTLYL"
     gene            2633..3811
                     /locus_tag="PLR93_04045"
     CDS             2633..3811
                     /locus_tag="PLR93_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNH26163.1"
                     /translation="MKQNSMQHAWQQWAWVWHAIAYIMMALNLTIALGNSLRRGSFQT
                     TAWLTILLAVWYLPFMALPARQSINTPWKLQLYFIIGWVIWMALIYFHNPAMMLAALF
                     YPQVFIRLPFRWAMVSAVLFTVSTFYLGFILPATPDALPTLYVVGVLLVSSQAILGGF
                     INSLISQSSQRYRLLNELEETRFNLLKAERETAILSERQRLSREIHDTLAQDFTSIIM
                     SLNAAKLTENSTAQVYLEQAEQTARDGLNDARRIVWALRLEELENNSFTEAIERLTAR
                     WSAETAIRVNSRFTGNPKPMRAEHEAALLRVTQEALHNIKKHAHAQEVNITLSYMPDL
                     FALDIADNGIGFEQRNLKGFGLKTMRERIEEIGGSLVIESQFGAGTTIAVSLPLEEQG
                     "
     gene            3808..4434
                     /locus_tag="PLR93_04050"
     CDS             3808..4434
                     /locus_tag="PLR93_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26164.1"
                     /translation="MTIRIMIVDDHAIVRSGLKAILASQQDFELVADVGTGDAAVSAS
                     ATLRPDVILMDLQMPVMDGVTAISLIHKKQPDIRMLVLTTYDTDADIVRAVEAGAIGY
                     LLKDALPDELFRAIRSAARGEVIFAPSVAAKLAKRLTNTLDRSLSNREIEVLILAARG
                     DSNKEIASKLHITEATVKSHFVHIFNKLSVTDRTAAVTLALEKKLIRL"
     gene            complement(4435..5130)
                     /locus_tag="PLR93_04055"
     CDS             complement(4435..5130)
                     /locus_tag="PLR93_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNH26165.1"
                     /translation="MSSQKPALDPMISSPDSNEYFNSISHLIGAILSISALAVLVSLA
                     AIDGKPAHVVGFAIYGTSLFLSFLFSCLLHFFLLFNKYYRVFGILDHNAIYILIAGTY
                     TPFCLTIFSGATGWVLFGVIWSLAVFFITLKSIFFAKIPVWLSQASFLLMGWIVVFLI
                     KPAYTQLGMDAIILMIAGGLSYTVGAISFAIGKPNPFPPYFGNHEIWHIYVLAGNTFM
                     FLVMLWHILPYPK"
BASE COUNT         1287 a         1102 c         1335 g         1426 t
ORIGIN      
        1 tatcaactta tcaaagacct tgccgtagtt atcggtggcg gcgacaagga aatcagtaaa
       61 gctgacattg gcggagtgat cggctttatc ggacatggca cggacgcaca ggaaaggtac
      121 tttgttgatc tcgcaggtgt gcgcgatggc ggcggcttcc atatctaccg cgagggcgtt
      181 gtgggtggag aggagttgac cggctttttc aacactgctg atgaactggt ctccgctgac
      241 aacgcgacct ttgtagactt tgggggcgtg tccgccaatt tcatccagtg aaacggattg
      301 ggcggattga aatgccaact cggtaagttt gggatcggtg gcaaaagcgt ggcgagacgt
      361 gaacccgagt tgccccgttt ctggtttagt gacgacatca aaaaggattt cgccgggttt
      421 cataccccag ccggtcaggt cgaagtcatg ttgaatggcg tcggtagcaa tgacaaggtc
      481 gccgacttca aggtttgggg cgagcgcgcc ggaactgccc atgttgaaga gtacatccgg
      541 ttcaaagaaa tcgagcagtt gctgagtagc gatcactgcg ttgaccttgc ctgggtcaca
      601 gcgggtgagg tagacctgat gtccgtgcat atcgccgtgc cagtaggtgc ggttgccgcg
      661 ttcgatcttt tcaagatggc tggagagtgc acggatgggg ataagctctt cgtctaatgc
      721 gccaatgata ccgattttca tgggaacgga tccttttgag ttaattttga ttattttact
      781 agctttgctt tccagtttgc gtaatgctca gccgtgtgtt gggcttgcca ttcgcaaacc
      841 tcccatagat cagctacagg ttgatttgta tgtggaatgg tgtgaagata aaggggcggt
      901 ccgtactcgg cggtgagggt tgtggcacta aatccgcggc gcgattgtgc atcccagacc
      961 atttgccacc aacgttcgtg cgcatccaag tgggtttgat actctagcgc acgcggatcg
     1021 ggcacctgcg gtccttcttc atacccgacc cgtgcatgga tatgcagaca atgctccgcc
     1081 gcggttttga tgtaagcttc ttcttcgttt agcagcagcc gttcgctgac acaaacccaa
     1141 tggctgaagt cacagcagag attaatgtca gggaaatctt tcatgacatc ctgtgtgacc
     1201 cacggattga acaagattcg tccgcgatgg gtttcgtggg atacccgggc ttttatccct
     1261 tgctcaaact tcagggcttc ggtgaaaaat tggtttcgtt ctattggagt ccaagaatca
     1321 cgtccgctgt gtgcgttgat gagatatggg tcgaaggata acgccaagtc gatctgtgct
     1381 tggaaggttt ggatatggtc gtttacggta tttccgctgg tgaaaatgag cggaatgtac
     1441 ttgagacctg tctctgaaag caggcttttg aatgatttcg atttttccgg ctcaggcaac
     1501 ggacattcca cacctgcata gcccaacgct ttgatcttcg gtaaaacact ttccagcggt
     1561 tcttcaacgc cccagaggga acgaaatttg aggtagtcca tgtgcgcctt tacttatata
     1621 gaagatttat taagaagttg gtaaaaccat atagttattt tacttgttct tttgtgctat
     1681 ccatcgaaag atggatagca aggtcaatct ctagagagat gtgttctaaa agcgatgagc
     1741 ctatactgca gctattcttt ttacaggagc ttgtatgaaa aaaataaatg ctgatttact
     1801 gtctctgatc gttattgctg cattctcatt tatgtttgca acagccatcc atgaacatgg
     1861 cgggcatgca ctggcatgcg tattgcaagg cggtcatatc tctgaaatgg gtgcttttta
     1921 tgtggattgt acgcgaacat ccttatctga atttgggaac cgtgtagtgg ctgtggctgg
     1981 acctttggtt agttttctaa ctgggttgat tgcattaggg tatttctatc gtaatcagcg
     2041 aacaaattct tcattgacct tttttctttg gcattttgcc acagtcaact taatgatggc
     2101 atctgggtat tttcttttct ctggtgtagc aggcatcggt gacttgggaa caggtgaaag
     2161 tggtgcattt caaggggtgc agccagaatg gttgatacgc atggggctgt ctgttttagg
     2221 gctggttttt tattatgctg tcatacgaat ctcaataaag atgatggatc atttcattgg
     2281 tggcgaaggt agagagcgag tagggcgcgc tcaaatgctc tccgtaattg catacctagt
     2341 ggggggtggt attgctattt tgatcggtct tttcaaccca tatggaatcg tcattgtatt
     2401 ggtctcatcc tttgcttcca gtatgggggg cacctctggt atggcatgga tgatgcagtt
     2461 gcttgaccgt tcaaaagaca caggcatgcc cgcgtttgca atgccgcgta attggtcgtg
     2521 gatttttgta ggagtgggat ttatcctttt atatgccatt atttttgggc gtaccttgta
     2581 tttataaagc ctgtgtacga aataactttt gctaatctct tataatgcca gtatgaaaca
     2641 aaattccatg cagcatgctt ggcaacaatg ggcttgggtt tggcatgcca ttgcctatat
     2701 catgatggca ttaaatctca ccatcgcatt agggaattcc ttgcgccgag gctcattcca
     2761 aactacagca tggcttacta ttttattggc ggtatggtac ctgccattca tggcgcttcc
     2821 tgcccggcaa tcgatcaaca ccccttggaa gctacagttg tatttcatca ttggctgggt
     2881 tatttggatg gcactaatct acttccataa cccggctatg atgttggcgg ctttgtttta
     2941 cccacaagtt tttatccgcc tgccatttcg ctgggcaatg gtcagtgctg tgttgttcac
     3001 ggtaagcacg ttttatctcg ggttcattct gcctgccaca cctgatgctt tgccaaccct
     3061 gtatgttgtt ggggttttat tggtttcttc gcaagctatt cttggcggct tcattaactc
     3121 cttgatctca caaagcagtc aacggtatcg tctgctcaat gagcttgagg agacgcgctt
     3181 taacctccta aaggctgaac gtgaaactgc aatcttgagc gagcgccaga gattatcacg
     3241 tgaaattcac gatacgcttg ctcaagactt caccagtatc attatgagtc tcaacgctgc
     3301 taagttaact gaaaacagca cggcacaggt gtatttagag caagccgaac aaaccgcacg
     3361 cgatggctta aacgatgcgc gtcgtattgt gtgggcatta cgcttggaag agcttgagaa
     3421 taattcattt acagaagcga tagaaagatt aacagctcgc tggtctgctg aaacagccat
     3481 ccgcgtaaac tcaaggttta ctggtaatcc caagcccatg agagcggagc atgaggcggc
     3541 gcttttgcgc gtcacgcaag aagcattgca taacattaag aaacatgccc atgcgcaaga
     3601 agtcaatatc acgctttcgt atatgccaga tttattcgcg ttggatattg ccgataatgg
     3661 catcgggttt gaacaaagaa atcttaaggg ctttggtctt aaaaccatgc gtgaacggat
     3721 agaagaaata ggtggctctc ttgtgatcga aagtcaattc ggtgcgggaa caacaattgc
     3781 cgtctctttg cctcttgagg agcaaggatg accatacgca ttatgattgt ggatgaccat
     3841 gcgattgtgc ggagtggatt gaaagcgatc ctcgcctcac aacaagactt tgagttggtc
     3901 gctgacgttg ggaccggaga cgccgctgtt agtgcttctg ccactcttcg ccccgatgtg
     3961 attttgatgg atttgcaaat gccagttatg gatggggtga ctgcaatttc gcttattcac
     4021 aaaaaacagc cagatattcg tatgcttgtt cttacaacat atgataccga tgccgatatt
     4081 gtacgggctg ttgaagctgg ggcgataggg tatctattga aagatgcatt acctgacgaa
     4141 ttgtttcggg caattcgttc tgccgcgcgt ggcgaggtga tttttgctcc cagtgttgcc
     4201 gctaaattgg cgaaacgctt aacgaatacg cttgataggt ctcttagtaa tcgcgagatt
     4261 gaagtgctta tactggcggc ccgcggtgac tccaataaag aaattgccag caaactgcat
     4321 atcacagaag ctacagttaa atcgcatttt gtgcatatct ttaacaaact cagcgtcaca
     4381 gaccgtactg cggctgtaac gctggcactt gaaaagaaat tgattcggtt gtagttattt
     4441 ggggtagggc agtatatgcc atagcatcac aagaaacata aacgtgttcc cagccaagac
     4501 gtagatgtgc cagatctcat gattcccaaa atacggtggg aaaggattgg gcttaccaat
     4561 ggcaaagctg atggcaccca cggtgtagct caacccgccc gcaatcataa ggataatcgc
     4621 atccatgccg agttgagtat aggcaggctt tattagaaag accacgatcc aacccatgag
     4681 caagaatgaa gcctgcgaaa gccagaccgg gatcttggcg aagaaaatag atttgagcgt
     4741 gatgaagaaa accgccaagc tccaaatgac gccaaagagc acccaacctg tcgcaccgct
     4801 gaaaatggtg agacagaaag gcgtatacgt gcctgcaatc aaaatataaa tggcattatg
     4861 gtcaaggatg ccaaagacgc ggtaatactt gttgaacagt aaaaagaaat gcagtaaaca
     4921 actaaatagg aacgatagaa ataaactcgt gccatagatg gcaaatccca ccacatgggc
     4981 gggcttgcca tctatggctg ccagtgaaac taatactgcc agtgccgaga tcgaaaggat
     5041 tgcaccgatg agatgactga tggagttgaa atactcgttg ctatctggcg acgagatcat
     5101 ggggtcgaga gcgggttttt gtgaagacat gggcgcgagt ataacttaac
//
LOCUS       DAOQFT010000108         5147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8363_length_5147_cov_7.054988, whole genome shotgun sequence.
ACCESSION   DAOQFT010000108 DAOQFT010000000
VERSION     DAOQFT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8363_length_5147_cov_7.054988"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..758
                     /locus_tag="PLR93_04060"
     CDS             87..758
                     /locus_tag="PLR93_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNH26166.1"
                     /translation="MLRDRISPNISEFLRYLASHPEADDKLPSLNELSRELNISLASL
                     REQLEVARALGFVEVRQKTGTRRLTYSFTPAIRQSLGYALALNDEHFRKFAEMRNHLE
                     AAYWYEAVKQLTDEDKKELNAIITRAWEKLQGTHVQVPHEEHRKLHLTIYKNLDNPFV
                     IGVLEAYWEAYEAVGLNVFTGGYEYLKEVWQYHQRMVESICSGKFEVGYEALVRHTDL
                     LYHRP"
     gene            833..2779
                     /locus_tag="PLR93_04065"
     CDS             833..2779
                     /locus_tag="PLR93_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNH26167.1"
                     /translation="MNKIVNDFSITVGTKNGSGSSTANNTILRSIFKMGIPVSGKNLF
                     PSNIQGLPTWYTIRVNKDGFIARKETSDIVIAMNPDSFAKDLASVTPGGAFFYADDIK
                     QPITRVDIAIYPMPVKTIVKNDPNVPTDFRELVGNMVYVGVLAQVIGMDMEAIRAALT
                     FHFKGKQKPIDMNLNAVKAGAEWAAANLEKKDPFYLEHMNKTEGLIMSDGNTAGAIGS
                     IFGGVQFAGWYPITPASSLAEALNDYLPVLRKREDGKHTYAVVQAEDELAALGMAIGA
                     GWSGLRSMTSTSGPGLSLMTEFAGLAYYAEVPVVIWDVQRIGPSTGLPTRTSQGDLSF
                     TAFIGHGDSHSVILLPGDVSECFEFGWKSFDIAERLQTPIFVLSDLDMGMNQWMSKPF
                     EYPEVPMDRGKVLWEKDLEEIKGNWGRYLDKDGDAIAYRTIPGNKHPLSSYFTRGTGH
                     DEYAKYTEDSGMYMKNMERLARKQQTAKEFVPAPVLHAKKGATVGILAFGSTENAIFE
                     AQAQLEDENGIKSELLRLRAIPFTKEVDAFIKKYDQIFVVEMNRDGQLLQLLYTEYPQ
                     HAMKFKSVAYHDGLPAAAKWVREGILAKYEKASSQKPAAIKKAAVKKTVAKAKAAPAK
                     KVAVKKSVSKKPAKSKSAGKAKRK"
     gene            2812..3867
                     /locus_tag="PLR93_04070"
     CDS             2812..3867
                     /locus_tag="PLR93_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNH26168.1"
                     /translation="MSETLPMAGAPANVNSVGLSKNDYRGAPSTLCQGCGHNSISNQI
                     ISAMYEMNVLPEDVIKFSGIGCSSKSPTYFMNRSFGFNSLHGRMPSIATGALFGDAHM
                     KGIGVSGDGDSASIGMGQFKHAMRRNVNMVYIVENNGVYGLTKGQFSATAEKGLQLKK
                     QGENPYMPVDICMEGLISNASFVARSFAGNPKQVKELLKAAFAHKGIAVLDIISPCVT
                     FNNQENAYHSYTWGKDHEEPLHEISYIAPRSEIMLEREMNEGEIREVAMHDGSTVILK
                     NLEKGYDPTNRMLALQALEEAQQNNWLLTGLIYVNPSKPALTDIYNLVDTPLNRLTET
                     DLRPDRAMIDKINALMF"
     gene            3930..4334
                     /gene="arfB"
                     /locus_tag="PLR93_04075"
     CDS             3930..4334
                     /gene="arfB"
                     /locus_tag="PLR93_04075"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNH26169.1"
                     /translation="MKNLENEISLEYIRASGPGGQNVNKVATAVQLRFDVVNSPSLGS
                     DLKSRLIQLAGKRVNAEGVLILEAKRFRTQEANREDAIAKLYEMIRKASEKPKTRHKT
                     KPTKASKEERMKEKKKRGAVKKLRQTKARFEE"
     gene            4402..>5147
                     /locus_tag="PLR93_04080"
     CDS             4402..>5147
                     /locus_tag="PLR93_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNH26170.1"
                     /translation="MHKHHTFAGLISGLTAASIWGGMYVVSKVVLEVIPPFTLLLIRL
                     IMGAAALGIVIYFRNRKSSTSTPVTKDIFWKSFLVGFVGYGISLGFQFVGTKLSTASN
                     GSLVTSATPAFVLLFAPFLLGEKPTTRRIIALVLSTFGVLAVIDPRTAELSPSLFWGN
                     MSLLAAALTWALYSVLVRKVSQTQDLLTSSTIMLLGGIPSSLAFSLWEINTQGVGEIT
                     LGIIGGLLFLGFISTALAMFLWNYAFAELPA"
BASE COUNT         1367 a         1202 c         1235 g         1343 t
ORIGIN      
        1 cttgaattaa ctaaactctt aaccaaaaat ccttgaccga aggctttcac gggcgtaaaa
       61 tacgattaat cggattaatc aattttatgc tacgtgaccg tatttcaccg aacatttccg
      121 aatttcttcg ttatctggcg tctcaccctg aagcagatga caagctcccc tctttaaacg
      181 aacttagccg agagttgaat attagccttg catcactacg cgaacaacta gaagttgctc
      241 gcgcgcttgg ctttgtcgaa gttcgtcaaa agactggaac gcggcgattg acttattcct
      301 tcactccagc aatccgccag agtttgggat atgccttggc actgaatgat gagcattttc
      361 ggaaatttgc agaaatgcgt aatcatcttg aagctgctta ttggtatgaa gcggttaagc
      421 aattgacgga tgaagataaa aaagaactca acgccatcat tacacgcgct tgggagaaac
      481 ttcaaggcac gcatgtgcaa gttccccatg aagaacaccg taagttacac cttaccattt
      541 acaaaaactt ggataaccct tttgtgatcg gtgtattaga agcgtattgg gaagcctatg
      601 aagcagtggg tttaaatgtg ttcacgggcg ggtatgaata tttgaaagaa gtgtggcaat
      661 accatcagcg tatggtcgag tcaatttgta gtggcaagtt tgaggtcggg tatgaggcgc
      721 ttgtacgtca tactgacctt ttgtatcatc gtccctaggt cattttcgtc gataggcaca
      781 gatgaggcta taaccgtctc tgttcatcga cctaattttt atgaggagtt ccatgaataa
      841 gatagtgaac gatttttcga tcaccgttgg tacgaagaac ggttccggca gttccactgc
      901 caataacacc attttgagat ccatctttaa gatgggcatc cccgtctctg gcaagaacct
      961 gttcccttcc aatatccaag gtctgcctac gtggtacacc attcgtgtga ataaggatgg
     1021 tttcatcgcc cgtaaggaaa ccagtgacat tgtcattgcg atgaaccccg attcgttcgc
     1081 aaaggacctc gcatctgtta cgccaggtgg ggcttttttc tacgcggacg atatcaagca
     1141 acccatcacc cgagtggaca ttgccattta cccaatgccg gttaagacaa tcgtcaagaa
     1201 cgacccgaat gtacctaccg attttcgtga attggttggc aacatggtgt atgtaggcgt
     1261 gcttgcacag gttatcggca tggatatgga agccatccgt gctgcgttga ccttccattt
     1321 caagggcaag caaaagccaa ttgatatgaa cctcaatgca gttaaagctg gtgctgaatg
     1381 ggctgctgca aacctcgaaa agaaagatcc cttctatctt gagcacatga ataagactga
     1441 aggcttgatc atgtcagatg gcaatacagc tggagcgatt ggctctatct ttggtggtgt
     1501 tcaatttgct ggttggtacc ccatcacgcc tgcttcttct ttagcagaag ctttaaatga
     1561 ttacttgcct gtgcttcgta aacgcgaaga tggcaagcac acttatgctg ttgttcaggc
     1621 agaagatgaa cttgcagcgc tcggtatggc aattggcgca ggttggtctg gtctgcgttc
     1681 gatgacttca acttctggac cgggtctttc tctcatgacc gagtttgcag ggttggcata
     1741 ctatgctgaa gttccagttg tgatttggga cgtccaacgt atcggtccca gcaccgggtt
     1801 gcccactcgc acctcgcaag gtgacttgag cttcaccgcc tttattggtc atggtgactc
     1861 acattctgtt attcttttgc caggtgacgt atctgaatgt tttgaatttg gctggaaatc
     1921 ttttgatatt gcagaacgcc tccaaacccc gatcttcgtt ttgagcgatc tcgatatggg
     1981 tatgaaccag tggatgagta agcccttcga atatcccgag gtcccgatgg atcgcggcaa
     2041 agtgctttgg gaaaaagacc tcgaggaaat caaaggcaat tggggtcgtt acctcgataa
     2101 ggatggcgat gccattgcat atcgcaccat tccgggcaat aagcacccgc tcagctcata
     2161 cttcacccgt ggaaccggtc acgatgaata tgccaagtat acagaagact ctggtatgta
     2221 catgaagaac atggaacgcc tcgcccgcaa acagcaaacc gccaaggaat ttgtccctgc
     2281 accggtgttg catgcaaaga aaggcgcgac ggtcggtatc ctcgcatttg gttctaccga
     2341 aaatgcgatc ttcgaagcac aggcacagct tgaggatgag aacggtatca agtctgagtt
     2401 gttgcgcttg cgcgccattc ctttcaccaa agaagtggat gccttcatta agaaatatga
     2461 ccagatcttt gtggtcgaaa tgaaccgcga tggacaattg ctccagttgc tgtacaccga
     2521 atatccgcaa catgcaatga agtttaagtc tgtggcatat cacgatggtt tgcccgcagc
     2581 agcaaaatgg gttcgcgaag gaattttggc aaagtacgag aaagccagca gccagaaacc
     2641 tgccgccatc aaaaaggctg cagtgaagaa aactgttgca aaggcaaaag cagctccggc
     2701 gaagaaagtg gcggtgaaaa aatccgtgag caaaaaacct gctaaatcaa agtccgctgg
     2761 caaggcgaag cggaagtagt tatcggtgaa tttaaaaagg agtaaataac aatgagcgaa
     2821 actcttccca tggctggcgc ccctgcaaat gtaaatagcg ttggcttgag caaaaatgat
     2881 tatcgtggcg cgcccagcac cttgtgccag ggctgtggtc acaactcgat ctctaatcag
     2941 atcatttcgg cgatgtatga aatgaacgtg ttgcctgaag atgtgatcaa gttctcgggt
     3001 attggttgtt cttccaaatc accaacctac ttcatgaacc gcagcttcgg tttcaactcc
     3061 ctgcacgggc gtatgccttc cattgcgaca ggcgcgttgt tcggtgatgc acacatgaaa
     3121 ggcattggcg tttctggcga tggcgacagc gccagcattg gtatggggca gttcaaacat
     3181 gccatgcgcc gcaatgtgaa catggtgtac attgtggaaa acaatggtgt gtatggtctc
     3241 accaaaggtc agttctctgc gaccgctgaa aaagggttgc aattgaagaa gcaaggtgaa
     3301 aacccataca tgccggttga tatctgcatg gaaggtctca tctcaaatgc aagctttgtg
     3361 gctcgttctt ttgcgggtaa ccccaaacaa gtgaaagaac ttctcaaggc ggcttttgcg
     3421 cacaaaggga ttgccgttct cgatatcatt agcccttgtg ttaccttcaa caatcaggaa
     3481 aatgcatatc actcctacac ttggggtaaa gaccacgaag aacctttgca tgaaatttct
     3541 tatatcgcgc cgcgtagtga aatcatgctc gaaagagaaa tgaacgaagg cgaaatccgc
     3601 gaagttgcca tgcacgatgg ttccaccgtc atcctcaaga atcttgaaaa gggctatgac
     3661 cccaccaatc gcatgctggc attgcaagct cttgaagaag ctcagcagaa taattggttg
     3721 ctcaccggtt tgatctacgt caacccatcc aaacctgccc tcacggatat ctacaatctt
     3781 gtagatactc ctctcaaccg cctgaccgaa accgatctgc gcccagaccg cgccatgatc
     3841 gacaagatca atgccttgat gttctagttt gctagcactt caacccctct ctgaaaagag
     3901 aggggttttt attttataat tttggcagaa tgaaaaatct agaaaacgaa atctcgctgg
     3961 aatatatccg tgcatcgggt ccgggaggac aaaacgtcaa taaagttgcg acggcggtac
     4021 aactgcgctt tgatgtggtg aattccccat cactcggctc agacttaaag agcaggctga
     4081 ttcagcttgc tgggaaacga gtcaacgctg agggcgtttt gattcttgaa gcaaagcgct
     4141 tccgcactca agaagcgaac cgcgaagacg ctatcgcaaa gctatacgaa atgattcgaa
     4201 aagctagcga aaagcccaaa acacgtcaca agaccaagcc caccaaagcc tcaaaagagg
     4261 aaagaatgaa agagaagaaa aagcgaggcg cggtaaagaa attacgtcaa accaaggcgc
     4321 gttttgaaga atagattcta tacatgccct gccctctaaa aagtgcaatt ttcaagtgtg
     4381 ggtattatat aattgagggc tatgcataaa catcacacat tcgcaggttt gatttctggg
     4441 ctcactgccg cctcaatttg gggcggcatg tacgttgtca gtaaggtggt tttggaggta
     4501 attcccccat tcaccctgct tcttatccgt cttattatgg gggcagcagc gcttggaatt
     4561 gtcatttatt ttcggaaccg aaaatcttca acctcaacgc cggttacaaa agatattttt
     4621 tggaagagct ttctagttgg gtttgtaggc tatggcattt cgctgggctt tcaatttgta
     4681 gggacgaagc tttccaccgc atcgaacggg tctttggtta cctccgcaac gccagctttt
     4741 gttttgctct ttgccccatt cctgcttggt gaaaaaccta caaccagacg catcattgcg
     4801 cttgtacttt ccacatttgg tgtgcttgcc gtcattgacc cgcgcacagc ggaactttcg
     4861 ccatcccttt tctggggcaa tatgagtcta ttagcagcgg cgttgacatg ggcactgtat
     4921 tcggtcttgg tccgtaaagt ttcgcaaacg caagaccttc tcacctctag cacgatcatg
     4981 ttgcttggcg gaatcccttc cagcttggca ttctcgcttt gggaaatcaa tacgcaaggt
     5041 gttggcgaga tcacacttgg catcattggc ggattgttat tccttgggtt tatctcaact
     5101 gcgcttgcca tgttcttatg gaattacgcc ttcgccgaat tgcctgc
//
LOCUS       DAOQFT010000109         5144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8370_length_5144_cov_9.211043, whole genome shotgun sequence.
ACCESSION   DAOQFT010000109 DAOQFT010000000
VERSION     DAOQFT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8370_length_5144_cov_9.211043"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLR93_04085"
     CDS             <1..597
                     /locus_tag="PLR93_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNH26171.1"
                     /translation="DPVRVITNHSSGKQGYALAQAALDAGAEVKLVTTPTALTPPVGA
                     DVVHVKSVQDMLDALLQESADALIMAAAGADFRPVNVAKDKLKKRDGIPQIQLEAAPD
                     ILKTLASQGVEKKRFQVTVGFAAESRDLLANASEKLKSKGLDFIAANDISANDAGFAV
                     ETNRITLLFANGTQETLPLMSKSEVAEKIIEHIARLLE"
     gene            599..1330
                     /locus_tag="PLR93_04090"
     CDS             599..1330
                     /locus_tag="PLR93_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNH26172.1"
                     /translation="MRILVMSDIHANYTALEAVLKDAGSVDETWCLGDLVGYGPDPNA
                     VVEEVQEIKNLTCLMGNHDVAVVGRMSLETFNGDAKRSLMYHEKVLTASNIDFIKSLP
                     SKTKVRGEASMAHGSPRDPLWEYILNSMTASLNFEHFDTPWCFVGHTHIQSSFSQVDA
                     SGRVLVEQTRPDVTLQLQPKLILNPGSVGQPRDRDPRAAYAIYDTEARTWTPKRVMYN
                     IAEVQERIRAAGLPEKHAVRIGEGW"
     gene            1468..2448
                     /locus_tag="PLR93_04095"
     CDS             1468..2448
                     /locus_tag="PLR93_04095"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNH26173.1"
                     /translation="MKRLTPYSLILIAMIVLGACASAPRAVELSGGSAPASEPYYDQS
                     SVMPAPTMMYATDGIIAEEAQVSGEGSGNANAAQVERIVIQNADIAVVVSDVELRMDE
                     IQKMAVDLGGFVVSSNLYQSYTSDYTPVPEASLTIRVPAENLDEALEKIKKDAVEVQN
                     ETRSGQDVTAEYVDLKSRLKTYQAAEEELTQLMDNAQNSDEVVNIFNQLMYYREQIEI
                     IQGQIKYYEEAAALSAISIRLIAEETTKPVTIGKWEPKGVALEAIQDLLDFWKGFVDF
                     MIRFVIFTLPMLITIGIPFYLAFIGVRWVFRKMRGNKKKVEPQVKEEEKN"
     gene            2508..3218
                     /gene="rsmG"
                     /locus_tag="PLR93_04100"
     CDS             2508..3218
                     /gene="rsmG"
                     /locus_tag="PLR93_04100"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNH26174.1"
                     /translation="MDSLIQDAKTLFNVHLTARQVVALVNYEKELMEWNQKFNLTAIR
                     DVESIRTKHFLDSYSCVLAWKANPPQRLIDVGTGAGFPGIPLKIIYPTMQVTLVESVG
                     KKAMFCQHVIQTLGLEGIEVLHSRAEDVGQNPAHRETYDWAVARAVANLNILSEYLLP
                     LVKLGGTMLAQKGETGPVEAQAAEKAMKILGGKLKQLIPVNLPGVVDDRYLVLTDKVA
                     ATPPKYPRKAGIPMKTPL"
     gene            complement(3226..3591)
                     /locus_tag="PLR93_04105"
     CDS             complement(3226..3591)
                     /locus_tag="PLR93_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26175.1"
                     /translation="MEWHIEYIPEIALLYVKTKGILTNEKANQMVAEIALAMKEHQCL
                     CQIVDHRETVFDFRTLDYYERPKVNQQLGMSKQWRVAMIFKQLSEETLFMETVFRNRG
                     FEFRQFDDLDKAKEWVLKK"
     gene            complement(3691..4023)
                     /locus_tag="PLR93_04110"
     CDS             complement(3691..4023)
                     /locus_tag="PLR93_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26176.1"
                     /translation="MKLSLKHYGIILGALLAAYFHLILFPDIGFTLNGLGYLGLLGAY
                     FLPIAFFQQKHKMVWWGLVGYTILTIVLWAIMGNKQFVAGTTSAIGYYAKAAEVFLLA
                     CLWADKPQ"
     gene            4227..4652
                     /gene="ssb"
                     /locus_tag="PLR93_04115"
     CDS             4227..4652
                     /gene="ssb"
                     /locus_tag="PLR93_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNH26177.1"
                     /translation="MYHTIIIVGNVGKDPEMRYTPSGQAVTSFSVATNRQYTAGNGEQ
                     VKETIWFRVSTWGKTAEICNQYVKKGSKVLIEGRLTPDKATGGPRIWSKQDGTAGASF
                     EVTAQTVRFLSSRDDGASGPIAGGGMDMAEIPPEDDIPF"
     gene            4751..5089
                     /locus_tag="PLR93_04120"
     CDS             4751..5089
                     /locus_tag="PLR93_04120"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNH26178.1"
                     /translation="MTNPQTPPKQPTGPILELPEGVEPEYVNLARIAHSPSDIVIDFA
                     HLLPGEPRAKIRSRVVMTPLSAKLLLKALAENIARYEAAYGEIHVPVNSSLADNLFRP
                     FQQPPEPPKE"
BASE COUNT         1422 a         1297 c         1215 g         1210 t
ORIGIN      
        1 gaccccgtac gcgtcatcac caatcattcc tctggcaagc aaggctatgc ccttgcccaa
       61 gctgcattgg atgctggcgc tgaggtaaag ctagtcacga ccccaacggc attaactccg
      121 cctgtgggtg cagatgttgt ccatgtgaaa agcgtgcaag atatgctcga tgctcttctg
      181 caagaatctg ctgatgcgct catcatggct gctgctggag cagatttccg cccggtgaat
      241 gtggcgaagg ataagctcaa aaaacgcgac ggtattccgc agatccagct tgaggcggca
      301 ccagacatcc tgaaaacgct ggcaagccaa ggcgtagaaa agaagcgatt tcaagtcaca
      361 gtaggattcg cagcagagag tcgcgacttg ctggcgaatg catccgaaaa gctgaaatcc
      421 aaaggcttgg actttattgc cgcgaatgac atctctgcaa acgatgccgg ttttgcagtg
      481 gagaccaatc gaattacgtt gttgtttgca aatggaacac aggaaacatt gccactcatg
      541 agcaaaagtg aagtggctga aaaaatcatc gaacacattg cgaggctgtt ggaataatat
      601 gcgtattttg gtcatgtcgg atattcatgc aaactatacg gcgttggaag ctgtattaaa
      661 agatgcgggg tctgtggatg aaacgtggtg ccttggtgat cttgttggat atggaccgga
      721 cccaaacgcg gtggtggagg aagtgcagga gatcaagaac ctgacttgcc tgatgggcaa
      781 tcatgatgtg gcggttgttg ggcgcatgtc gttagagaca ttcaacggcg atgccaagcg
      841 ttcgttgatg tatcacgaga aagtgcttac cgccagcaat attgatttca ttaagtctct
      901 gccgagcaaa accaaggtgc gcggagaagc gtctatggcg catggcagtc cgcgcgaccc
      961 gttgtgggaa tacatcctca actcgatgac cgcgagtttg aacttcgaac atttcgatac
     1021 cccttggtgc tttgtggggc atacccacat ccaaagtagt ttttctcaag tagatgcttc
     1081 aggacgtgtg ctagttgagc agaccagacc cgatgtgacg cttcaacttc agccgaaact
     1141 gattctgaat cccggcagtg ttggtcaacc ccgagaccgt gacccgcgtg ccgcttacgc
     1201 catttatgac accgaagccc gcacttggac gccaaaacgt gtgatgtaca acatcgccga
     1261 agtgcaagag cgcatccgcg cggcgggatt gccagaaaaa catgccgtga gaattggcga
     1321 aggttggtaa cgcctcccac aaaagtcctc cctgaagttc gggagggctt tttctttata
     1381 ggaagttgta caatacgtgg gaacaattta ttttagcgta gcgtcctttg aagagagaaa
     1441 ttaccaaagg aaaagaagga gaaagaaatg aaacgcctaa cgccctatag tcttattttg
     1501 attgcaatga tcgttcttgg agcttgtgcc agtgctccac gcgccgtgga actttctggc
     1561 gggtctgccc cagcttcgga gccctattat gaccaatcct ctgtaatgcc cgcaccgacc
     1621 atgatgtatg ctactgatgg cattatcgct gaagaagccc aagtaagtgg agaaggcagc
     1681 gggaacgcca atgccgccca agtggaacgt attgtcattc agaatgcaga tattgcagtt
     1741 gtggtctcag atgtggaact gcgtatggat gagatccaaa aaatggcagt cgatcttggc
     1801 ggattcgtag tgtcttccaa tttgtatcag agctatacca gtgattacac cccggtgcct
     1861 gaagccagcc tgaccatccg cgtgcctgct gaaaatctgg atgaagcgct ggaaaagatc
     1921 aagaaagatg cggtggaagt tcagaacgaa acccgctctg ggcaagatgt caccgctgaa
     1981 tacgtggact taaagtcccg cctcaagacc tatcaagccg ctgaagaaga actgacccaa
     2041 ttgatggaca atgcacaaaa ctcagatgag gtcgtcaata tcttcaatca gttaatgtac
     2101 taccgcgaac agatcgagat cattcagggt cagatcaagt attacgaaga agctgccgct
     2161 ctctctgcca ttagcattcg tctcatcgcc gaagagacca ccaaaccagt aacgattggc
     2221 aaatgggaac cgaagggtgt tgcgctcgaa gccatacaag atctgctcga tttctggaaa
     2281 ggcttcgtcg atttcatgat ccgcttcgtg atcttcacct tgcccatgtt gatcaccatc
     2341 ggtattccgt tctacctggc attcatcggc gtccgctggg tcttccgtaa gatgcgtggc
     2401 aacaagaaaa aagttgaacc gcaggtgaag gaagaagaaa agaattaaaa cgtcgtaaca
     2461 cgagaggacg gtgtaccgcc gtcctctttg ttttataatc ttcgcatatg gactctctga
     2521 ttcaagacgc aaaaacatta ttcaacgtcc acctcacggc gcgacaggtg gtggcgctcg
     2581 tcaattacga aaaagaattg atggagtgga accaaaaatt caaccttact gccattcggg
     2641 atgtagagtc tattcgcacc aagcattttc tggattcata ttcctgtgtg ctggcgtgga
     2701 aagccaaccc gccgcaacgc ctcatcgacg tgggcacggg cgctggtttc cccggcattc
     2761 cgcttaagat tatttaccct accatgcaag tcaccttggt tgaatcagtc ggcaagaaag
     2821 ccatgttctg tcagcatgtg attcaaacgc tgggcttgga aggcatcgaa gtcttgcatt
     2881 ctcgtgccga agatgtaggt caaaaccccg ctcatcgtga aacctacgac tgggctgtgg
     2941 cacgggctgt ggcgaatctc aacatcttga gcgaatatct tctgccgctt gtcaaactcg
     3001 gcgggacgat gcttgcccaa aaaggcgaga ccggacccgt tgaagcacag gctgccgaga
     3061 aagcgatgaa aattttaggt ggaaaattaa agcaattgat ccccgtcaac ttgcccggtg
     3121 tagtagacga tcgttatctg gttctaacag ataaagtcgc tgctacacca ccgaagtatc
     3181 cccgcaaggc aggcatcccc atgaagacgc ctttatagct tgcgtctatt ttttcaaaac
     3241 ccactctttg gctttatcca aatcatcgaa ctgacgaaac tcaaatccac gattccgaaa
     3301 aacagtttcc ataaacaagg tttcttcaga gagttgtttg aatatcatgg cgacacgcca
     3361 ctgtttagac atccccaact gttggtttac ttttgggcgt tcataataat caagggttct
     3421 gaaatcaaaa accgtctcac gatgatccac gatctgacag agacattgat gttctttcat
     3481 tgcaagcgca atctcagcaa ccatctgatt tgctttttca tttgtaagta tccccttggt
     3541 ctttacatat aaaagggcga tctcggggat atattcaata tgccattcca tacggctacc
     3601 gcctaaaaag aagcatggta tgacaataaa gtcataccat gttatgaact actacaatag
     3661 cggcggattt taccagataa cgcaaaaaga ttactggggt ttatctgccc aaagacacgc
     3721 taaaaggaaa acctcagccg ccttggcata gtatccaatg gcactggtcg tccctgcaac
     3781 gaattgttta tttcccataa ttgcccatag cacgatggtg agaatggtat aaccgaccaa
     3841 accccaccaa accattttat gtttctgctg gaagaaggca atcggtagaa aataagctcc
     3901 gagcagcccc agataaccta atccattcag ggtgaagcca atatcaggga aaaggataag
     3961 gtggaagtac gccgccaaca atgcccctag aatgatcccg tagtgcttaa gacttagttt
     4021 catgctttaa ctccttgggt gattggaatt acgacgaata cttaacgcat ggacacccca
     4081 cctcagcctg ttcttacatg cgtgtatttt cacgataaaa cccaaattga ttaacaaata
     4141 aagcaagaaa gagtacaata caaatgtttc aattgtttcc cgtcgaaatt cggcgggcac
     4201 ctttaaccca agaggagaat ccccccatgt atcacactat tattatcgta ggtaacgtcg
     4261 gtaaagaccc cgaaatgcgt tacaccccat cgggtcaggc tgtgacctcg ttctcggtcg
     4321 ctaccaatcg acagtacact gccggaaatg gcgaacaggt caaagaaacc atttggttcc
     4381 gtgtttccac ctggggcaag accgcagaaa tctgcaatca gtacgtcaag aaaggctcca
     4441 aggttttgat cgaaggacgc ctcactccag acaaagccac gggtggtcca cgcatttgga
     4501 gcaaacaaga cggcaccgcc ggtgcatcct ttgaagtcac cgcacaaacc gttcgcttcc
     4561 tctcctcacg cgatgatggc gccagcggac ccatcgcagg cggcggcatg gacatggcgg
     4621 aaattccccc cgaagacgat attccattct aatcacctaa ccctcattac cacagagccc
     4681 acaacctata cagagaacgc aagacctttc tccgtaaacc cgtgatctct gtggtaaaaa
     4741 atttaatctc atgaccaatc cccaaactcc cccaaaacaa ccaaccggtc ccatccttga
     4801 acttcctgaa ggtgtggaac ctgaatatgt gaacctcgca cgcatcgctc actccccatc
     4861 tgatattgtg attgactttg cgcatttact gcccggcgaa ccacgcgcca agatccgctc
     4921 gcgagtagtg atgaccccgc tcagtgccaa gcttctattg aaagcattgg cagaaaacat
     4981 cgcacgctac gaagccgcat atggcgaaat ccatgtgcct gtcaactctt ctctggcaga
     5041 taatctcttc cgcccgttcc aacagccgcc tgaaccgcct aaagaataac catgcacaaa
     5101 cttgaatcca tcgccgatca aattcgcaag aattttgatg ctcg
//
LOCUS       DAOQFT010000110         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8399_length_5134_cov_6.014964, whole genome shotgun sequence.
ACCESSION   DAOQFT010000110 DAOQFT010000000
VERSION     DAOQFT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8399_length_5134_cov_6.014964"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..746)
                     /locus_tag="PLR93_04125"
     CDS             complement(120..746)
                     /locus_tag="PLR93_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26179.1"
                     /translation="MLIHPSPYERITALTDIFLGILAAWGAAQLFAFADFRHQIWAWT
                     FCLLAIASFLGAIAHGFAMSTKTNDRLWMPINVALGLTLGLFVVGALFDLSGESVARQ
                     ALPVMIALGFGFFLITILKSGTFMTFIAYEALAMLFALGIYGYLFFTSNLLGVGWMVL
                     GVLVTIIAAVVQATGKAGKSIFWYFDNNGVFHLIQMIGMVLLYFGLNT"
     gene            complement(740..1657)
                     /locus_tag="PLR93_04130"
     CDS             complement(740..1657)
                     /locus_tag="PLR93_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNH26180.1"
                     /translation="MDSTPIPSSTPQPTATSAPVPNFPVIGYLPDYRELNPAWASSLT
                     DIIYFSAEPRADGSLDTSRLTEETWQALASLKDENGIRVHISIGGWGRDGAFVEMSAD
                     PETRKTFIANLLNFLQERQLDGADFDWEFPQNDTEFEQYIALLTETKAALSPHGLLVS
                     VALPAESSFPLGEFAVVDRVHLMSYDRGERHSTFEQSVADVEAFLEAGIPPEKLILGM
                     PFYGRGMSSPSDTYSYEQIRSTYAPAPDVDEVGDIYFNGLNTIRHKVCYGIAQNLGGF
                     MVWELGLDTVDESSLLQQIYRLAQGAESC"
     gene            1760..2323
                     /locus_tag="PLR93_04135"
     CDS             1760..2323
                     /locus_tag="PLR93_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26181.1"
                     /translation="MLKPTDLILVCLMPTPRDMEIARLLGWYRIPLKSAPKVVSVDYL
                     AFYQPTSFGERGGQVEYIAQVRGHELTTRGELLKDEADHPRAKEEYFKLQLGNLEKLK
                     EPVRTDKWKRLTFLYSTGEYLLNAKTLNDLVVEGEERNMLWKNLRERAENDQLYQVDL
                     PEADIPPEVLMALLGIKDLAGGYDAGQ"
     gene            2409..4079
                     /locus_tag="PLR93_04140"
     CDS             2409..4079
                     /locus_tag="PLR93_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNH26182.1"
                     /translation="MPADLMVVSPSSSLGEAIRRSLEDTKFYRVTTVNNKAAAIVKAN
                     EIGMPMAMLDLALGEEWVAEIGASLRTIRPSINLVILCEEAGSPPAFDSLRPWIMVRK
                     PFKISDFMNAISQPQSAQPAKSGAPAPAMPMSSTAQFMPWLNDPNKAAQHLTRLTLES
                     SAQAALITKKNDLWAYAGGLSQNAAKEVAQTVTRNWDGQKGSDLLRFIRLESTKAEHM
                     LYATRLTSDAVLALVFDAETPFSTIRSQASQLLSDFGSEKAEPKNQPAVSYAVSDDDS
                     KDEDLNIPPISNILADIPAPNPEPLTTRDFNLPRKTPAQPQPQEAFDPNQTRVSDALS
                     NASVFSRDPSPAVRRDSVPQPMAQTQKSEAHHDTQIDFDEVQVTAPSQPKARPETPVA
                     RPRPGENDATRESPSTEMARKLTAEPISAGLYHLTYACLLVPRFSSHYLTGDLSDRLG
                     EWLPNICIAFGWRLEFLAVRPEYLQWVVNVQPNTSPGHLMRIMRQQTSEKIFGDFPRM
                     KRENPSGDFWAPGYLIMGGSQPHPPQLVRDYIRQTRQRQGLEDLPPMK"
     gene            4186..>5134
                     /locus_tag="PLR93_04145"
     CDS             4186..>5134
                     /locus_tag="PLR93_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNH26183.1"
                     /translation="MPPTLYLIDGHALAYRMYFALTAGGGSQRWQTSKGEPTAGIYGF
                     ARELVRVLEQEKPEYLAVAFDVGKTFRDQIFPEYKATREKMPDDLRSQIARIREMVDA
                     FNIPRLEMDGYEADDVLGSVAKLAAEKGLGVKIITGDRDLLQLVNERTAVYLAGDDQT
                     YITDADVIKKLGVRPNQVVDYKAIVGDTSDNIPGVKGVGEKTAVALLEKFNTLDAIYA
                     NLDQVENRWRGKFETNKETAYMSRDLARIETDLKIKFDFEHAKVAPFDGSKLEAFFKE
                     MEFRTLVTKVPAISGGVASAPAETKAPTVKTGKTKAGQMT"
BASE COUNT         1375 a         1365 c         1281 g         1113 t
ORIGIN      
        1 aattgatgag ttgatcgtcg agttcaataa agaagttggg ttgtcgtaaa agaaaagtag
       61 gggcgcggtc tccgcgccca atacgttttc cacaataatg gggcggggta accccgcccc
      121 tacgtgttta atccaaaata caacaaaacc atcccgatca tctgaatcaa gtgaaagacc
      181 ccgttattat cgaaatacca gaagatactc ttgccagctt ttccagtcgc ctgcaccacg
      241 gcagcgatga tcgtcaccaa caccccaagc accatccacc ctacacccag caaattagat
      301 gtaaaaaata aatagccgta tatgcccaat gcaaagagca tagcaagcgc ttcataggcg
      361 atgaaggtca taaaagttcc ggactttaag atagtaatga ggaaaaagcc gaagcctagc
      421 gcgatcatca caggcaacgc ttggcgagcc acgctttcac cacttaggtc aaacaatgcc
      481 cccacaacaa acaagcctaa ggtcaaccct agcgctacat taatcggcat ccataaccgg
      541 tcatttgttt tggttgacat agcaaatccg tgggcgatgg caccgagaaa agaggcgatt
      601 gccagcaaac aaaaggtcca tgcccaaatt tgatggcgga aatcggcgaa ggcaaaaagt
      661 tgagccgcac cccatgctgc cagaatccct aaaaagatat cggtcaatgc agtgatacgt
      721 tcatacgggg aaggatgaat taacatgact ctgctccttg tgccaagcga tatatctgtt
      781 ggagtaatga actctcatcc actgtatcta gccctaactc ccaaaccatg aatccaccca
      841 agttttgcgc gatgccataa caaactttgt gccgaatggt gttcaagccg ttgaaataaa
      901 tatcccccac ttcatccacg tctggtgcag gcgcataagt agaccgtatc tgttcataag
      961 aataggtatc agatggcgaa gacattccac gcccatagaa aggcatgcct aagatcaact
     1021 tctcaggtgg aatacctgct tctaaaaaag cctccacatc tgccacggat tgctcaaagg
     1081 tggagtgtcg ttcgccgcgg tcataagaca taagatgcac acgatcaaca accgcaaact
     1141 ctccaagtgg gaaactcgac tcagctggga gggcaacact caccaacaag ccatgcgggg
     1201 aaagagcagc tttagtttcg gttaataagg ctatgtactg ttcaaactct gtgtcgtttt
     1261 gtggaaattc ccaatcgaag tcggcaccgt caagctgacg ctcttgtaga aagttcaaca
     1321 agttagcaat aaaggtttta cgagtttcag ggtcggcgct catttctaca aaggcaccat
     1381 ctcgccccca gccgccaatg gagatatgta cgcgaatccc gttttcatcc tttaacgatg
     1441 ccaatgcctg ccatgtctct tctgttaagc gtgaagtatc cagtgagcca tcggcgcggg
     1501 gctcggcaga gaagtagatg atatcggtca aggaagatgc ccatgcgggg ttcagctcgc
     1561 gataatctgg caaatagccg atgacgggga aatttggcac aggcgctgag gtggcggtcg
     1621 gttggggcgt ggacgagggt atgggagttg aatccacttc gggcgcggga agaggcgtag
     1681 cccctcctcc acaagcaact agtagagaac atgatagaag gaggaatgag agtcgcatgg
     1741 tgaacattat aattaagcca tgctcaaacc tacggacttg atccttgttt gcctgatgcc
     1801 tacaccgcgc gatatggaaa ttgcgcgttt gctgggatgg tatcgcatcc cattgaagag
     1861 cgcgccgaaa gttgtcagtg tggattatct tgcgttttac caacccactt cgttcggcga
     1921 aagaggcggg caagttgaat atatcgctca agttcgtggg catgagttga caaccagagg
     1981 cgaactgctc aaggatgaag ctgatcaccc aagagcaaaa gaagagtatt tcaagttgca
     2041 gttgggaaat ttggagaagt tgaaagagcc ggttcgaacc gataaatgga aacggctgac
     2101 cttcttgtat tccacaggcg aatatttgct caacgccaaa accttgaatg acttggtggt
     2161 agagggcgaa gagcggaaca tgctttggaa gaacctgcgc gagcgagccg aaaatgacca
     2221 gctttatcaa gtggatttgc ccgaggcaga tattccgcca gaggtgctga tggcgctctt
     2281 gggcatcaaa gacctggcag gcggctacga cgcggggcag taagttgtcc agaagagtta
     2341 ctaaaatgat aagaaaccac ccaaaggttt ccttgcatgc gtataacaga agtgatacac
     2401 tttaaccgat gcctgctgat ttaatggttg tttccccttc tagtagcctc ggtgaagcca
     2461 ttcgccgttc gctggaagac accaagttct accgagtcac taccgtcaac aacaaggctg
     2521 cagcgatcgt caaggcaaat gagatcggaa tgccgatggc gatgctcgac cttgcacttg
     2581 gcgaagagtg ggttgcagaa attggcgcat ctttacgcac cattcgcccc agcatcaacc
     2641 ttgttatcct ctgcgaagaa gcaggctccc cgcctgcctt tgattcatta cgtccatgga
     2701 tcatggttcg caaaccgttc aaaatctctg attttatgaa tgccatcagt caacctcaat
     2761 ctgctcaacc agcaaagtct ggcgctccgg ctccagccat gccgatgtcg agcacggcgc
     2821 agttcatgcc gtggctgaat gaccccaaca aagcggctca acaccttacc cgtctcacct
     2881 tggaatcttc ggcgcaagcg gcgctgatca ccaagaaaaa tgacttgtgg gcatatgcag
     2941 gcggtctctc tcaaaacgcc gccaaagaag ttgcgcaaac cgtcacccgc aattgggacg
     3001 gacaaaaagg ttctgacctt ctgcgcttta ttcgactcga aagcaccaaa gcggagcaca
     3061 tgctctacgc cacgcgcctc acttcagacg ccgtgctggc tcttgttttc gacgccgaaa
     3121 ctccctttag caccatccgc tctcaggcaa gccaattgct gagcgatttt ggcagtgaga
     3181 aagcggagcc caaaaatcaa cccgccgtct cctatgctgt cagcgatgat gatagcaagg
     3241 atgaagactt aaacatcccc cccatctcga acattctcgc ggatattccg gctccgaacc
     3301 ctgagccact taccacacgc gatttcaatt taccgcgcaa gacccctgcg caaccccaac
     3361 cgcaagaagc attcgacccg aatcaaaccc gcgtttcgga tgcgctttcc aacgcttcgg
     3421 tctttagtcg cgacccatcg cctgctgttc gcagagattc ggttccgcag cccatggcac
     3481 agactcaaaa gtctgaagcc catcacgata cgcaaatcga tttcgatgaa gtgcaagtga
     3541 cggctccttc tcagcccaag gcgcgacctg aaacaccggt cgcacgtcca cgccccggcg
     3601 agaatgacgc cactcgcgaa agcccctcta ccgagatggc gcgcaaactg accgcagagc
     3661 cgatctcggc tggcttgtat cacctgacct acgcttgttt gttggtgccg cgcttctcct
     3721 cccattacct gaccggcgat ctctcagacc gccttggcga atggctgcct aacatttgca
     3781 tcgctttcgg ctggcgcttg gagttcttag ccgtgcgccc ggaatatttg caatgggttg
     3841 tgaacgtgca gccgaataca tcgcccggtc acttgatgcg cattatgcgc cagcagactt
     3901 cagaaaaaat ctttggcgat ttcccgcgca tgaagcgtga aaacccctct ggtgatttct
     3961 gggcaccagg ctacctcatc atgggcggtt cacaaccgca cccgccacaa ctggtgcgcg
     4021 attacattcg ccaaacccgt cagcgccaag gtctggaaga cctgccacct atgaagtagg
     4081 tttctgaaac aaaagttcta aataaggaaa cgcagagtat aattaactct gcgttttttc
     4141 ttttagacac tattccaatt ggtcactaca cgactggata ctgacatgcc ccctaccctt
     4201 tatctcattg atggacacgc tctcgcctac cgcatgtact tcgccctgac agcaggcgga
     4261 ggcagccaac gttggcagac ttcgaaaggt gaaccgacag ctggtattta cggctttgcg
     4321 cgtgaactgg tgcgcgtgtt ggagcaggaa aaacctgaat accttgccgt tgctttcgac
     4381 gtaggcaaaa ccttccgcga ccagatcttc cctgaatata aagcgacccg cgaaaagatg
     4441 cccgatgatt tgcgttcgca gatcgcgcgc attcgtgaga tggtggatgc gttcaatatt
     4501 ccgcgcctcg aaatggacgg ctacgaagcc gatgatgtgc tgggcagtgt ggcaaagctc
     4561 gcggcggaaa aaggtctcgg cgtgaaaatc attaccggag acagagacct gctacagctt
     4621 gtcaatgagc ggacggcggt gtatctggca ggtgacgacc aaacctacat caccgatgcc
     4681 gatgtcatca aaaagctggg cgtccgcccg aatcaagtag tggactataa agccatcgtc
     4741 ggcgatacgt cggacaatat tcccggcgta aagggcgtgg gcgaaaagac agcggttgcc
     4801 ttgctggaaa agttcaacac gctggatgcg atctatgcca atctcgacca ggtggagaat
     4861 cgctggcgcg gcaagtttga gacgaacaaa gaaacggcgt atatgagccg tgacctggcg
     4921 cgcattgaaa cagatctaaa aatcaaattc gattttgaac atgccaaggt ggcgccgttc
     4981 gacggctcga aactggaagc cttcttcaaa gaaatggaat tcagaacact ggtgaccaaa
     5041 gtgcctgcca ttagtggtgg cgtggcttct gccccagcgg agactaaagc tcccaccgtg
     5101 aaaaccggca agaccaaagc cgggcagatg acga
//
LOCUS       DAOQFT010000111         5122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8432_length_5122_cov_6.286165, whole genome shotgun sequence.
ACCESSION   DAOQFT010000111 DAOQFT010000000
VERSION     DAOQFT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8432_length_5122_cov_6.286165"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLR93_04150"
     CDS             <1..780
                     /locus_tag="PLR93_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNH26184.1"
                     /translation="HLDIAIPAAEAGCAILLEKPIAGSMERVDVLQKTVKKSGSKVLV
                     AFQFRFHPGMVKTRELIENGEIGRVVSASVHFGEYLPAWHPWEDYRQGYAARADMGGG
                     VVATQCHSLDYLPWLVGKKVESVWGFADKLSDLEVTADDTAKIGLRFEGGAVGSLHLD
                     YNQQPPEHEFRIIGTNGTIKWNLVDGAARIYRTEKKDWDVYPLPEGWERNVMFQQQTK
                     HFVDIVNGKAQPSCTLEDGVQVQRIIASVHESQETGKLIKL"
     gene            1019..2746
                     /locus_tag="PLR93_04155"
     CDS             1019..2746
                     /locus_tag="PLR93_04155"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HNH26185.1"
                     /translation="MAKANLITPYGGKLVDLVVKGAERDALIARAGHLPSIKITMRNL
                     CDLELIATGGFSPLTTFMGKADYERVLKEMRLADGTLFPLPITLTADPKELPTVGEEL
                     ALRNANFDLIAVMRLDEVYHWDANTEAALAYGSTDTKHPMVSEMGRWGKVCISGPLQV
                     INLPKYYDFVNLRHTPAQVREMLEAMGNDNVVAFQTRNPLHRIHEELTKRAAAQVNGS
                     LIVHPVVGMTKPGDVDHYTRVRTYKALVDNHYDQKSTMLSLLPLAMRMAGPKEALLHA
                     IIRRNHGANHFIVGRDHAGPGNDSTGKPFYGPYDAQELMTKHEAELGVKMVPFEMLVY
                     LPDEERYVEEKDVPKGAKVANISGTQVRDDYLAKGKLLPEWFTRPETAEILREMYPPR
                     HKQGFGIWFTGLSGSGKSATTAVLTTLLLERGRETAILDGDVVRTHLSKGLGFSKEDR
                     DTNILRIGFVAGEIVHAGGIVICAAISPYRATREEARKMVGENFIEVYMDTPVEVCEQ
                     RDVKGLYAKARQAMADGKPMGFTGVDDPYEPPVKPEITLKGYESTPEDNARAIIKYLE
                     EQGFLLPVK"
     gene            2795..4345
                     /locus_tag="PLR93_04160"
     CDS             2795..4345
                     /locus_tag="PLR93_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNH26186.1"
                     /translation="MTKSHSASFLILGLLALSGTFLILYATPQGMGLFDDSIAYIAGA
                     RSIVAGQGYREAWLASNQPVTHFPPGFSTVLALVGISGLDPLRGTRLVNAVVFGANIF
                     LLGLIGWRMTKSKLAGMALSALLLLSGELFRVHTTAMSEPLYIFFTLAAFLAFSQTFV
                     SEEKQTNKFLLLASALTAFAYLTRYAGLALFATFLVSLFLLHPTWKQRLTSAGIFLAG
                     FVPFALAWAVRNRLLTDNATNRKLVYHPITPENIQLGISNFASFILPIEEWRKVLIKV
                     PNLVAVILIVLILALLGWVVLKGSQKFFNPASPTPEVLSFVSTLYVFGYLASILSSMT
                     LFDASTKFQLRILAPVYVSLLIMLVYFGYWLWQKQKIALILLSLVAFSFSVSSFLSTF
                     TQLRKGGQGYASFQWFDSEAMKFMQNLPAETRIYSNETAAVYLYTDHPGYVLPDLIDP
                     VTGLPRGRFDEGVKALQEDVLSGNAVLALFDVDSQDEDVQSTYQILANGLYLAFDKQG
                     DKIYTAYP"
     gene            4380..>5122
                     /locus_tag="PLR93_04165"
     CDS             4380..>5122
                     /locus_tag="PLR93_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH26187.1"
                     /translation="MTIFDKFSLKDRVAVVTGGGGQLGFEFCKTLAEAGAAVVAADLN
                     MDFAGKTATRLTEAGYAAMAFPLDVTRPESTRELVAETVKQFGRLDILVNSAALDPKF
                     DPTAAEKGIAPGAFEDYPLADWQAALNVNLTGMFLTTQACVKQMVSQGKKGSVINICS
                     TYGLNGPDQRIYIKDGKRVAYKPVYYTTTKAGVMGFTKYLAAYYAETEIRVNALTPGG
                     VYNNHEEYFVKNYSAKTILGRMAKKDEMNG"
BASE COUNT         1193 a         1272 c         1304 g         1353 t
ORIGIN      
        1 catttggata ttgccattcc tgccgcggag gcgggctgtg ctattttgct ggagaaaccc
       61 attgctggct caatggagcg cgtggatgtt ttgcaaaaga ctgtgaaaaa aagcggctcg
      121 aaggtgttgg tggcgtttca gttccgcttt cacccgggaa tggttaagac ccgtgaactg
      181 attgaaaacg gtgagattgg gcgggttgtc tcggcaagcg tgcatttcgg tgagtatttg
      241 ccagcttggc acccgtggga agattatcgt cagggatatg cggcgcgggc tgatatgggt
      301 ggaggcgtgg ttgcaacaca atgtcactca ttggattatt tgccgtggtt ggttgggaag
      361 aaggtggagt ctgtttgggg ctttgcagac aaacttagcg accttgaagt gactgcggat
      421 gacacagcca agatcgggct gcgatttgaa gggggcgcag taggaagcct tcacctagac
      481 tataaccaac agccgccaga gcatgaattc cgcattattg gcacaaatgg gaccattaag
      541 tggaatttgg tggatggagc ggcgagaatt taccgcacag agaaaaaaga ttgggatgtg
      601 tacccgctac cggaggggtg ggagcggaac gtcatgttcc aacagcaaac gaaacatttc
      661 gtggatatag tcaacggcaa ggcacagcct tcttgtacac ttgaagatgg ggtccaagtg
      721 cagaggataa ttgcttcggt gcatgagtct caagagacag ggaagttgat aaagttataa
      781 gtgagaaagc ggcagagaac ctgtcgcttt tttgttggat tcgctttcag ggatgggaaa
      841 gtttggaagg tgtggcaggc tcgaggtcca gaaagaggcg gtgggtttcc aaaattcacc
      901 cactgaattt tggagtataa ttggcgcttg tgcaaactgg tatgggcgat ggatttccat
      961 gcatccccat actttatgcc cacgggcatt atctaaaaca aatggagtaa caatcacaat
     1021 ggctaaagca aatcttatta ccccttatgg tggaaaatta gtggacttgg tcgtgaaggg
     1081 tgccgaacgc gatgcattaa ttgcccgtgc tgggcatctg ccttccatca agatcaccat
     1141 gcgtaacttg tgcgaccttg aattgatcgc tacgggtggt ttttcgcctt tgacgacttt
     1201 catgggcaaa gcggactacg aacgtgtgct caaagaaatg cgccttgcag atggaacgct
     1261 ctttccgctg ccgattacat tgactgctga cccgaaagaa ttgccgaccg tgggtgaaga
     1321 actcgcgctt cgcaatgcaa actttgactt gatcgctgtg atgcgcctcg atgaggtcta
     1381 tcactgggat gcgaataccg aagcggcttt ggcatacggc tcgaccgata ccaagcaccc
     1441 gatggtttct gaaatgggac gttggggaaa agtgtgtatc tctggtcctt tgcaggtgat
     1501 caaccttccc aagtattatg actttgtaaa tctgcgccac accccggctc aagtgcgtga
     1561 aatgcttgaa gcaatgggta acgataatgt ggtcgctttc caaacccgca atcctttaca
     1621 ccgcatccac gaagaattga ccaaacgcgc tgcggctcag gttaacggtt ctttgatcgt
     1681 tcaccctgtg gtgggtatga ccaagcccgg cgatgtggat cactacaccc gcgtccgcac
     1741 gtacaaggca ttggttgaca atcactacga tcagaagagc accatgctta gcttgttgcc
     1801 gctcgccatg cgcatggcgg gtccaaagga agcgttactg catgccatta tccgccgcaa
     1861 tcatggcgcg aatcacttca tcgttgggcg cgatcatgcg ggccctggca atgactcgac
     1921 gggcaaaccg ttctacggtc cttacgatgc gcaggaattg atgaccaaac atgaagcgga
     1981 actcggcgtg aagatggttc cgttcgaaat gcttgtgtat cttcccgacg aagagcgcta
     2041 tgtggaagag aaggatgttc ccaagggtgc caaagttgcc aacatctctg gcacacaggt
     2101 gcgtgatgat tatcttgcca agggcaaact actccccgag tggttcaccc gccctgaaac
     2161 cgctgaaatt ttgcgcgaaa tgtatcctcc gcgccacaag cagggcttcg gcatttggtt
     2221 cactggtttg agcggctctg gcaaatctgc caccaccgct gtcctcacca ccctgttgct
     2281 cgaacgcgga cgtgagaccg ccatccttga tggggatgta gttcgtaccc atctgtccaa
     2341 gggacttggt tttagcaagg aagaccgcga taccaacatt ctccgcattg gctttgtggc
     2401 tggcgagatc gtgcacgccg gtggcatcgt catctgcgcc gctatcagtc cgtaccgcgc
     2461 cacccgcgaa gaagctcgca agatggtcgg tgaaaacttc atcgaagttt acatggacac
     2521 tcccgttgaa gtttgtgaac agcgcgatgt aaaaggtttg tatgccaagg ctcgtcaggc
     2581 gatggcagat ggcaaaccga tgggcttcac gggcgttgat gatccgtatg aaccgcctgt
     2641 caagcccgag atcacactca aaggctatga atccacgccc gaagacaatg cgcgtgccat
     2701 catcaagtat ttggaagaac aaggcttcct gcttccggtg aagtaattgc tattcaggtg
     2761 acagtcacca ttatggtgac tgtcacctcg ttgtatgacc aaatcccact ccgcttcatt
     2821 cttaatcctc ggtctgcttg ctctttcagg gacctttctc atcctgtatg cgactccaca
     2881 aggcatggga ctcttcgatg attccattgc ttatattgcc ggtgcgagaa gcattgtagc
     2941 gggacaaggc tatcgtgaag cgtggcttgc ttctaatcaa ccagttacac acttccctcc
     3001 aggtttttca actgttttag ccctcgtcgg catcagcgga cttgaccctt tgcgtggcac
     3061 acgcttggtc aatgctgttg tgtttggcgc aaatatcttt ttgctcgggc tcattggctg
     3121 gcgcatgacc aagtcgaaat tggctgggat ggctttgtct gctttgcttt tgttaagcgg
     3181 ggaacttttc cgcgttcata ccacggcaat gagcgagccg ctgtatatct tttttacact
     3241 ggcggcattt ctagccttct ctcaaacgtt tgtctctgaa gagaaacaga caaacaaatt
     3301 tttgttactg gcgtctgctc ttaccgcttt tgcttaccta acccggtatg ccggtcttgc
     3361 cctctttgcg acctttctcg tttcactctt ccttcttcat cccacttgga agcaacgcct
     3421 aactagcgca ggtattttcc tcgcaggctt tgttcctttt gcgcttgctt gggcggttcg
     3481 taatcgctta ctgaccgaca atgccaccaa ccgcaagctg gtctatcacc cgatcactcc
     3541 tgaaaatatt cagcttggca tttctaattt cgcctctttt attttgccca tcgaagaatg
     3601 gcgtaaggtt ctgatcaaag ttccaaatct ggtcgctgtt attcttattg ttcttatttt
     3661 ggctttattg ggttgggttg tgctaaaagg gtcgcaaaaa ttttttaacc cggcttctcc
     3721 aacccctgaa gttctttcct ttgtcagcac gctttatgtt tttggctatc ttgcatccat
     3781 cctttcttcg atgacattgt tcgatgccag caccaaattc caactccgca tccttgcgcc
     3841 agtgtatgtc agcctgctca ttatgttggt ctattttggg tactggcttt ggcaaaaaca
     3901 aaaaatagcc ctcattctct tgtcactggt cgccttctct ttttctgttt ctagtttttt
     3961 gagtaccttc acccaactcc gcaaaggcgg gcagggatat gcttccttcc agtggttcga
     4021 ctcagaagcg atgaagttca tgcaaaatct tcctgcagag acgcgcatct attcgaatga
     4081 aacggcggct gtatatcttt atacggacca ccctgggtac gtcttgcccg atctcattga
     4141 cccggtcacc ggtttgccgc gtgggcgttt tgacgaagga gtcaaagcat tgcaagaaga
     4201 tgttttatct ggaaatgccg tgctggcgct gttcgatgtt gattcgcagg atgaggatgt
     4261 acaatcaacg tatcaaattc tcgccaatgg tttgtatctc gcttttgaca aacagggcga
     4321 taaaatctat accgcatatc cgtaaggcga ttctatccct tacacaaaaa ggaattcaga
     4381 tgactatttt cgataaattc agtttgaaag accgtgtggc tgttgtcact gggggcggtg
     4441 gacaacttgg ttttgaattt tgcaaaaccc ttgctgaagc cggagccgct gtggttgcgg
     4501 cagacctcaa tatggatttt gctgggaaaa ccgcgacccg cctcaccgag gctgggtacg
     4561 ccgccatggc atttcccctc gatgtgaccc gccccgaatc gacccgcgag ttggtcgctg
     4621 aaaccgtcaa gcagtttggt cgccttgata ttctcgtcaa ctccgcagct cttgacccaa
     4681 aatttgaccc cactgccgct gaaaaaggca tcgcccccgg cgcctttgaa gattatccac
     4741 ttgcagactg gcaagccgcc ctcaacgtca acctgactgg catgttcctc actacccaag
     4801 cctgtgtgaa acagatggtg tcgcaaggta aaaagggcag cgttatcaac atctgctcta
     4861 cctacggctt gaacggacct gaccaacgta tttacatcaa agacggcaaa cgggtagcct
     4921 ataaacccgt ctattacacc accaccaaag ccggtgtgat gggttttacc aagtaccttg
     4981 ccgcttacta tgctgagacg gagatccgcg tcaatgcatt aacccccggc ggtgtttaca
     5041 acaaccacga agaatatttc gtcaagaatt attctgccaa aactatcctt ggacgcatgg
     5101 caaagaaaga cgaaatgaat gg
//
LOCUS       DAOQFT010000112         5075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8536_length_5075_cov_7.174303, whole genome shotgun sequence.
ACCESSION   DAOQFT010000112 DAOQFT010000000
VERSION     DAOQFT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8536_length_5075_cov_7.174303"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PLR93_04170"
     CDS             complement(<1..1397)
                     /locus_tag="PLR93_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26188.1"
                     /translation="MKKISHLLLVLLILLSIPARSVYADVAPPETVPGSNLVPGGEST
                     QVRMVAETVTLTISQDPADEKRAMAETRAVFTMRNLGTAEESMAVRFPLSFFDGASDG
                     FFNYPELPSIAVKVDGKTVPTRREMQPFLASQSNSSDRDEIPWSVFDVTFPPNQDVTI
                     EVVYNVDGFGDYPYEVFKYVLETGAGWNGTIGSAEIILRLPYEANTYNVWTEGVEGYG
                     DATPGGVFSGNEVHWKYTELEPTFENNLQFVIVTPSAWGKVLKEDENVTQNSEDGEAW
                     GRLAKAYKELILMRHGNLRTDPAGQSMFELSRSAYETCLGILPNDPLWLYGSAELLWA
                     HYYFDFYMMGQPDTQNLLATTLTRLNSALEIDPNLQVAKDKLEEISYSIPEAVQKNED
                     GSFALIGLTATPAPLVWKVNVTPTIPPVPTPTATFTMGMIPPNTPEPTASNPICGGAF
                     LLPALFGLVLSVKRKR"
     gene            1552..2373
                     /locus_tag="PLR93_04175"
     CDS             1552..2373
                     /locus_tag="PLR93_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNH26189.1"
                     /translation="MQLTDTHCHLDYHKYNTDRADVIQRAKDAGLVKVLVPGLHHRSS
                     QEAVKLAESNTMVYAAVGFHPTDLDEFSEKAFLQVKELAKHPKVVAIGEIGIDYYWVK
                     EPEKRAVQREVLKQHLAFAQEVNKPVVLHMREQDDAWFGEASVDLLEILSEWQNGLSG
                     ELREKPGVLHSFNGTLETAQKALSLNFYLGVTGPVTYPSAEKKREVISQLPLDRLLIE
                     TDGPYLTPAPHRGKRNEPAYVVHIADKIAEIHSKSPAEIAQITTANAKRLFAWGD"
     gene            2375..3340
                     /locus_tag="PLR93_04180"
     CDS             2375..3340
                     /locus_tag="PLR93_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH26190.1"
                     /translation="MNSVTFLSPVTEEIKLIEDRMRIQADDSHPDLRAALKHLLAAGG
                     KRIRPTLGMLVGNMLGAPLEKLINLGAAVELLHTATLVHDDLIDGSLLRRGTPTLNAR
                     WSPPATVLTGDFLFARAAKLAADTDYLPLMKLFSETLAIIVNGELTQMFTSRGLINRD
                     NYYKRIYAKTASLFEMTSRSAAMVSPASDDSIEAMRDFGYQIGMAFQIVDDILDFNGD
                     QNAVGKPLGSDLLNGLVTLPAIYFAEINPDDPDVMSLPQGGWTNNENMTRLVEKIRAS
                     SCTKKAMVEAEQHVDRALVCLESFEPCAERDALENLAKYIVDRKV"
     gene            3407..3916
                     /locus_tag="PLR93_04185"
     CDS             3407..3916
                     /locus_tag="PLR93_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNH26191.1"
                     /translation="MTNPRKPFRFNVGFIIHEEVGYNHDFPFEFEKVTLNGDLELRNL
                     EGNVNVGKTPQGLIVQGKFTAETTVTCSRCLTDVVQDLEWEFTEMYAFDQRSITESGL
                     LLPEDAQLDVESLLREYALLEVPINPICKPDCLGLCIECGQNLNEKDCGHRPEEPDSP
                     FAKLKDLLK"
     gene            3924..>5075
                     /locus_tag="PLR93_04190"
     CDS             3924..>5075
                     /locus_tag="PLR93_04190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNH26192.1"
                     /translation="MPPAYAGGFHFSMKLSIPKPIRILITILVLVVLIFIRIRINLGF
                     DVPNSNFSFFWLAGRMILDGQNPYDETQYLAGHDANSMDWRPNNIFPYPLPLALFCIP
                     LGFFSMQTAYVIWQIVTLLLIALTIYLLLQHWQEESQQNLLLPIFFFLLFLGPVYLST
                     HAGTISILPVIVLVGAILLVEKEQSLWAGVVLALTMLKPPQGLTILILAGVWFLARRD
                     WKAIIGVALGGLAILVVGLIQDPQWIQKFLGAGDAVMARTLGVHSNVWAFAYLACKGA
                     EPCSNLLGGALSLSLLGGASLLLWKNQAKWSAWEAMNLIIPIGFVSTIYLWAYDQLPY
                     IIPIVWIVATLAQKQRSILLAFFFLILLDLASIFALVQQAFTEKDLWSLG"
BASE COUNT         1285 a         1293 c         1203 g         1294 t
ORIGIN      
        1 cgtttacgtt ttacactcaa taccaaccca aataatgccg gcaggagaaa tgctccccca
       61 caaataggat tggaagccgt aggctcaggg gtgttgggag gaatcatgcc catagtgaat
      121 gtagccgtag gtgtagggac aggcggtatg gttggggtga cattaacctt ccacacaagc
      181 ggagcgggtg tagcggtgag accaatcaaa gcaaaagacc cgtcttcatt tttctgaacc
      241 gcctctggga tagagtagct gatttcttca agtttgtctt tcgccacttg cagattgggg
      301 tcaatctcta aggctgaatt gaggcgtgtc aacgtcgttg ctaagaggtt ttgagtatcg
      361 ggctgcccca tcatataaaa gtcgaagtaa taatgcgccc acaacaactc agcagacccg
      421 tataaccaaa gtggatcatt tggcaagata ccaagacagg tttcataggc gctacggctt
      481 agctcaaaca tactctgccc tgcagggtct gtgcgcaagt tgccatgcct cattagaata
      541 agttctttat atgcttttgc aagcctgccc caagcttcac catcttctga attctgggtg
      601 acgttctcat cttctttcag cacctttccc caagcagatg gagtcacaat cacgaattga
      661 agattatttt caaatgtggg ttctaattct gtgtacttcc aatggacttc attcccgcta
      721 aacacgccac cgggtgtggc atcgccatag ccttccacgc cttctgtcca tacattgtag
      781 gtatttgctt catagggaag gcgcagaatg atctcggcac tgccaatagt gccattccaa
      841 ccggcgcccg tttcaagaac atatttgaag acttcataag gataatcgcc aaagccatcc
      901 acattgtata caacctcgat agtgacatct tgattgggcg gaaaggtcac atcgaaaaca
      961 gaccaaggaa tttcatcgcg gtctgaagag tttgattggc tggctaaaaa gggttgcatc
     1021 tcacgccgag ttggcacagt tttcccatcc acctttacgg caatagaggg aagttcagga
     1081 tagttgaaaa agccatccga tgcaccatcg aaaaaagaaa gcggaaaacg cactgccatg
     1141 ctctcttctg ccgttcctaa attgcgcatg gtgaaaacgg ctcgtgtttc agccatagct
     1201 cttttttcgt ccgccgggtc ttgagagata gtgagtgtga ccgtctctgc taccatgcgg
     1261 acttgggttg attcgccgcc gggcacaagg ttcgagccag ggacagtctc cgggggagcc
     1321 acgtcggcat aaacagagcg agcgggaata gacagcaaaa tcaggagaac gagcaagaga
     1381 tgcgatatct ttttcatagg gaacctatat tgtttcctac tatacaagaa tgaatcccat
     1441 tggtcaattc ggcaaaagta ccagacgaat gtcaccaata cctcacttat tttgtaaaaa
     1501 tcatccattt aacattttgc cctaaaaaca gactgatata atgccaacgc tatgcaattg
     1561 accgacaccc actgtcacct tgattatcat aaatacaaca cagaccgcgc agatgtaatc
     1621 caacgtgcaa aagatgcagg gttggtaaag gttctggtac caggactcca tcacaggtcc
     1681 agccaagaag cggtgaagtt ggcagagtcc aacacaatgg tgtatgcggc agtcggcttc
     1741 catcctacag atttggacga gttttctgaa aaagcgttct tacaagtgaa ggaattggca
     1801 aagcacccca aagtggttgc cattggcgag atcggcattg actactattg ggtcaaagag
     1861 ccagaaaaac gcgccgttca acgcgaagtt ctcaaacaac atttagcctt tgcgcaagaa
     1921 gtgaacaaac ccgtggttct tcacatgcgc gaacaagacg acgcctggtt tggcgaagcc
     1981 tctgtggatt tgttagagat cttgagcgag tggcaaaacg ggctaagcgg agaattgcgc
     2041 gaaaaacccg gcgttttgca ctcgttcaat ggcacgttag agaccgccca aaaggcgctg
     2101 tcactgaatt tttacctcgg tgttacgggt ccagtgacat acccaagcgc agagaaaaaa
     2161 cgcgaggtga tttctcaact tccgctcgat cggctgctca ttgaaacaga cggtccttac
     2221 cttacacctg ccccacacag gggtaaaaga aacgagcccg cctacgtcgt tcatattgct
     2281 gataaaatag cagaaatcca ttccaaaagc cctgcggaga tcgcccaaat taccactgcc
     2341 aatgccaaac ggctcttcgc atggggagat tgacttgaac tcggtaacct ttctttcacc
     2401 cgttacagaa gaaataaaac tcattgaaga tcgcatgcgg attcaggcag atgatagcca
     2461 ccctgacctg cgtgccgctt tgaaacactt attggcagcc ggagggaagc gcatccgccc
     2521 gaccttgggc atgcttgttg gcaatatgct gggtgcccca ctggaaaagc tcatcaacct
     2581 cggcgccgcc gtagagcttt tgcacactgc caccttagtc cacgatgatt tgatcgacgg
     2641 ctcgttactt cgccgcggca cgcctaccct gaacgcacgc tggtctccac ctgcaaccgt
     2701 gctcaccggc gactttctct ttgcccgcgc cgccaagctc gccgcagata cagactactt
     2761 acctctgatg aagttgtttt ctgaaacctt ggcgatcatc gtcaatggcg aattgacgca
     2821 aatgttcaca tcacgtgggc tcatcaaccg cgataactac tacaagcgca tttacgccaa
     2881 aaccgcctcc ctcttcgaaa tgacctcccg ctctgctgca atggtcagcc cggcaagcga
     2941 tgactcaatt gaagccatgc gcgatttcgg ctatcagatc gggatggcat tccagatcgt
     3001 ggatgatatt ttggacttca atggcgatca gaatgctgtg ggcaaaccgc tcggttcaga
     3061 cctactgaat ggcttggtca ccctcccagc catttacttc gctgaaatca accccgacga
     3121 cccagatgtg atgtcgctgc cccaaggcgg ttggaccaat aacgaaaaca tgacccgcct
     3181 cgtggaaaag atccgcgcca gcagctgcac caagaaagcc atggttgaag ccgaacaaca
     3241 tgtagatcgg gcgctcgtct gccttgaaag ctttgaaccg tgtgccgaac gcgatgcctt
     3301 ggaaaacttg gcaaagtata ttgttgatag aaaagtctaa gaatcaggaa gcctgattta
     3361 acagaccctg atttactagt ttcttattcc taatccctta tttcccatga ccaaccctcg
     3421 caaacccttt cgctttaacg tcggcttcat tattcatgaa gaagtcggct ataaccacga
     3481 ctttccgttc gagttcgaga aggtcactct caacggtgac ttggaactcc gcaacctgga
     3541 aggcaatgtc aacgttggca aaacccctca gggattgatc gttcaaggca aattcaccgc
     3601 tgaaaccacc gtcacttgtt cacgttgtct caccgacgtt gtacaagacc ttgaatggga
     3661 attcaccgaa atgtacgcct tcgaccaacg gtctattaca gagtctggtt tgctgttgcc
     3721 cgaagatgct caactagatg tagagagcct tctgcgcgag tatgcgcttt tagaagtgcc
     3781 catcaacccg atttgcaaac ccgattgtct cggtctgtgc attgaatgcg gtcaaaacct
     3841 caacgagaaa gattgcggtc accgccccga agaacctgat tcgccttttg caaaattgaa
     3901 ggacttgctt aagtaaaacc tgcatgccac ctgcttacgc gggtggtttt catttttcta
     3961 tgaaactttc cattcctaag cctattcgca tcctcatcac aatcctcgtt ctggtagttt
     4021 tgatcttcat ccgcattcgg attaacctcg ggttcgatgt ccctaactcc aatttttctt
     4081 tcttctggct ggcagggcgg atgattttgg atggacaaaa cccatacgac gagactcaat
     4141 atctcgcagg gcacgatgct aacagcatgg attggcgccc aaataatatt ttcccttatc
     4201 ccctaccctt ggctttattc tgtattccgc tcggcttctt ttccatgcag acggcgtacg
     4261 tcatttggca gattgttacg cttttgctga ttgccctgac catttacctt ctgttgcaac
     4321 actggcaaga ggaaagtcag cagaatttat tgctgcctat atttttcttc ttactctttt
     4381 taggtccggt ctacctgagc actcatgcag ggacgatcag catcctacct gtgatagtgt
     4441 tggtaggtgc aattctctta gtggaaaaag aacaatcttt atgggcgggt gtcgtccttg
     4501 cgttgacaat gctaaaaccg cctcaagggc ttaccatttt gatccttgcc ggggtatggt
     4561 tccttgcacg gcgtgactgg aaagcaatca tcggcgtggc gcttggcggg cttgcgattc
     4621 tcgtggtggg tttgatccaa gacccgcagt ggattcaaaa attcctcggc gcaggcgatg
     4681 ccgttatggc gcggactctc ggagtgcact cgaacgtttg ggcgtttgcc tatctcgcct
     4741 gcaagggagc ggagccatgc tcaaatctat tgggcggggc attgagtttg agtctgcttg
     4801 gaggggcgag tttgctcctt tggaagaatc aagccaaatg gtcggcatgg gaagcgatga
     4861 acttaatcat cccgattggg tttgtgagca caatttatct ctgggcgtat gaccagcttc
     4921 catacatcat cccaattgtt tggatcgtgg ctacattggc gcaaaagcag cgtagtattt
     4981 tgctagcatt cttctttttg attttgctgg atctggcttc gatctttgcg ctcgttcagc
     5041 aagcttttac cgaaaaggac ttgtggagcc taggc
//
LOCUS       DAOQFT010000113         5051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8601_length_5051_cov_6.046237, whole genome shotgun sequence.
ACCESSION   DAOQFT010000113 DAOQFT010000000
VERSION     DAOQFT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8601_length_5051_cov_6.046237"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..843
                     /locus_tag="PLR93_04195"
     CDS             16..843
                     /locus_tag="PLR93_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNH26193.1"
                     /translation="MTNMEEQSANNPFLIDSLNHPLAGPIRKLLTREGRRELNQILID
                     DEENILQAIEAGVEIDSLYFAGEETVSETLRKNLTAGATIHEVAKRTTKKLFENDKIS
                     RIFAIAYTPQPVGLEALKSIKKDVVVLEDLSISGNIGNITRTSLALGVGGILLLNMDP
                     VDLYDRRLIRASRGYLFSLPMITASTADFLDYCKKNNESLMVMDMHADKTVDDIAATP
                     HRLLLAFGSEKDGCSAEIENAATMKVRIPISDKVESLNVSASAGITLYSRSKFNQAN"
     gene            935..1351
                     /locus_tag="PLR93_04200"
     CDS             935..1351
                     /locus_tag="PLR93_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNH26194.1"
                     /translation="MKLATLPALVFLSLAMFVFNACSNSGTTLTGEWKLISYGDATNI
                     TPAIANAETSITFNNGKFGGTVGCNSFGADYTSEGSSIHIGSVVSTMMFCEETSAQES
                     AVLGIVSDKTLNVSMNGDRLTLTSEDGKSVIVLEKK"
     gene            complement(1384..2169)
                     /locus_tag="PLR93_04205"
     CDS             complement(1384..2169)
                     /locus_tag="PLR93_04205"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNH26195.1"
                     /translation="MQTLKLLSAFFKVNVQMSLAYRADTIVNILLNLMWLGWELLSLN
                     IIFNNTDTLGGWGMGELIALLGVFRLVNTMMIALIWPNTEKFNQSIRDGSMDYTILQP
                     VNSMFLVTFSRITVWRIWDLILAAVLIVVGVNMSAETTTAAQILTFILLAISGMIVIY
                     SLWIVLIALTFWFVKFDNNVTILQALMDSGRYPATVYPAWLRLIVTFIIPIAVATTVP
                     LQALRGELTIVQIFIFLGVSVASFLIASQVWKSGLKQYSGASS"
     gene            complement(2171..2968)
                     /locus_tag="PLR93_04210"
     CDS             complement(2171..2968)
                     /locus_tag="PLR93_04210"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNH26196.1"
                     /translation="MFLIFKRLWQVNWAEQWQYRANLLMYLLYWLVSPIIYLAVWTSI
                     AKVKGDVNGFTANDFITYYMVLLICDQFTSNIIIHTFAYKIQDGSLSGELTKPIHPML
                     TGALTNNVSFKVLSMMGLAPIWFVLFLLFRPDFSSVTFNNVLLALPVLIVAFFIGYLL
                     SATITSLAFWTTRVYSVHEFYFAFILLFSGQFVPLPLMPKLVQDIAQYLPFQLLLYFP
                     IQLILGKLPTEQIVMGYIMAGVWLVLSILIFNLVWRNGVKRYSAVGA"
     gene            complement(2983..3960)
                     /locus_tag="PLR93_04215"
     CDS             complement(2983..3960)
                     /locus_tag="PLR93_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH26197.1"
                     /translation="MSPTIQVQHLSKTYQVPEREGGFGAAVRSFFKRKYKEVKAVQQV
                     DFTIEQGEVVGFLGPNGAGKTTTLKMLSGLLHPTGGSANVLGFTPWELKPEYLRSMTL
                     VMGQRNRLSWDIPAADSFLLNQAIYRIPDDEYKETYKELDELLELEPLMKKPVRNLSL
                     GERMKCEIAAGLLHRPKVLFLDEPTIGLDITGQGRIREFLREYNKRTGATILLTSHYM
                     ADVTALCERIIIIHHGQLKYDGGINDLSKKIAPFKLIGVMLADADSHDLSKYGEVVQN
                     EEDAEKRYIQVKAEEVTRVTSRLLADLPIHDITIADPPIEDVIEQAFNA"
     gene            complement(4018..4659)
                     /locus_tag="PLR93_04220"
     CDS             complement(4018..4659)
                     /locus_tag="PLR93_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNH26198.1"
                     /translation="MEHKPDTIKVSASHKEEIFATHADLHVVVKGSSLVSGNEAMKKA
                     KEVNQLLEALKQAGVKEEAVTLQGVNVSTSTGAVLKSSTASYRLKIRCEDLAKFAEIL
                     DVIASQKNTSLTQIEWKYDEDVTREALLLMTLEKAKSKAAKVAQTMGVKLLGVYDLIE
                     NIYDEETAVPYQALAKPAFRSAPTEQEPSLGMDVQHSKTIHVNVDIWYRVSEF"
     gene            complement(4683..>5051)
                     /locus_tag="PLR93_04225"
     CDS             complement(4683..>5051)
                     /locus_tag="PLR93_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26199.1"
                     /translation="PLNRNIDGMSVKERLPSTFIHGLLGIGFAIANLLVWKWVILVGA
                     IWYSVVLLSAIRNWWIAYFFNIHQGEITPEIYEQHYSRNVRILPRFNRNPVIPDVQHI
                     LIHVSVLLAVIASWSSFFAA"
BASE COUNT         1381 a         1267 c         1188 g         1215 t
ORIGIN      
        1 cgttagggcg ttatcatgac caatatggaa gaacaatcag ccaacaaccc ttttcttatt
       61 gattcgctca atcacccgct ggcgggacct attcgcaaac tactcacccg cgaagggcgc
      121 cgggagctca atcagatact cattgacgat gaagagaata ttctgcaagc tatcgaagcc
      181 ggagtggaga tcgactcgct ttactttgca ggcgaggaaa ctgtctctga aacactgcgc
      241 aagaatctca cagccggggc gaccattcac gaagtagcca aacgcaccac aaaaaagctc
      301 tttgaaaatg acaaaatttc gcgcatcttt gcgattgcct acacgccaca acctgtcggg
      361 ttggaggcgt tgaaatccat caaaaaagat gtggttgtgc tcgaagattt gagcatctct
      421 ggcaatatcg gcaatatcac ccgtacttcg ctggcactcg gcgtaggcgg aattctcctg
      481 ctcaacatgg accccgtgga tctgtacgac cgccgtctca tccgcgcgag ccgggggtat
      541 ctcttctccc tgcccatgat caccgcatcc actgcagact ttttggacta ttgcaaaaag
      601 aataacgagt cgctcatggt catggacatg cacgccgaca aaacagtgga tgatattgca
      661 gccactcccc accggctctt gcttgccttt ggcagtgaaa aagatggctg ttccgcggag
      721 atcgaaaatg ccgctacgat gaaggtgcgc atccccatca gtgacaaagt ggagtcgctc
      781 aacgtttcgg cttcagcggg aatcacactc tacagccgct caaaattcaa tcaagccaat
      841 tagtccaaaa gaaccgagga aaacctcggt tctttttttg cgggaacttt ttctccatcg
      901 gggcgtcaaa aatatatatc aaggagaaga aattatgaaa ctcgccacct tacccgctct
      961 tgtcttttta tctcttgcca tgttcgtgtt caacgcctgc tcgaacagtg gcaccaccct
     1021 tacaggtgaa tggaaactca tctcatatgg ggatgctacg aacattacgc cagccattgc
     1081 aaatgcagag acatccatca cttttaacaa tgggaagttc ggcggcactg taggatgtaa
     1141 ttccttcggc gcggactata cgtcagaagg aagcagtatt cacattggct cggtcgtttc
     1201 gaccatgatg ttctgcgaag aaacctctgc tcaggaaagt gctgtacttg ggatcgtctc
     1261 tgataaaact ctgaatgtat ccatgaacgg cgatcgcttg actcttacaa gcgaagatgg
     1321 aaaatccgtg atcgtgctgg aaaagaaata aaaaaacggc gaccgtctgg tcgccgtttt
     1381 catttaactg cttgctccac tatattgttt gagccctgat ttccaaactt gggaggcaat
     1441 caagaaactc gcaacactta cccctagaaa gataaagatc tgcacgatgg tcaactcgcc
     1501 gcgcaaggct tggagtggaa cggttgtggc tacagcaatg ggaatgataa aggtgacgat
     1561 gagacgcagc catgccgggt aaacggttgc cgggtagcga cccgaatcca tgagggcttg
     1621 caggatggtg acgttgttgt cgaatttgac aaaccagaac gtgagggcaa tgaggacgat
     1681 ccaaaggcta tagatgacga tcatgcctga gatggcgagc aagataaagg tcaggatctg
     1741 agccgcggtt gttgtctcag cggacatatt tacgcccacc acgatcagta ccgccgcaag
     1801 aattaaatcc caaattcgcc aaacggtgat gcgcgaaaat gtgacgagga acatgctgtt
     1861 gaccggttgc aggatggtgt aatccatcga gccatcacga atggattggt tgaatttttc
     1921 ggtgttgggc cagatgagcg cgatcatcat cgtattgaca aggcgaaaga ctcccagcag
     1981 agcgatgagt tcacccatgc cccaaccgcc aagagtatcg gtattgttga agatgatgtt
     2041 gagagagagc aattcccagc cgagccacat taggttgagc aaaatgttga cgatcgtatc
     2101 agcacgatat gccaaagaca tttgcacatt cactttgaag aaagccgaaa gtaatttgag
     2161 ggtttgcata ctaggctccc actgcggagt aacgcttcac gccattgcgc cacaccaagt
     2221 tgaagatgag gatggacaag accaaccaca cacccgccat gatgtacccc atcacgatct
     2281 gttccgttgg cagtttgcca aggatcaact ggattggaaa atacaaaagc aactggaacg
     2341 gcaaatattg agcgatatct tgaaccaact tcggcatgag cggcaacggc acgaactgac
     2401 cggagaacaa gaggatgaac gcgaagtaaa attcatgcac agaataaacc cgcgtcgtcc
     2461 agaaagcgag tgacgtgatc gttgctgaaa gcagatagcc aataaagaac gctacaatca
     2521 acaccggcag ggcgagtaaa acattattga aagtgacact gctgaaatct gggcggaaca
     2581 acaagaacag cacgaaccag atcggtgcca gtcccatcat gctgagtacc ttgaaagaga
     2641 cattattggt cagtgcgccc gtcagcatgg ggtgaatggg cttggtcaac tcgccagaaa
     2701 gcgagccatc ttggatttta taagcaaaag tatggatgat aatgttgctg gtgaattggt
     2761 cgcaaatgag caagaccatg tagtaagtaa tgaagtcatt cgcagtaaac ccgttgacat
     2821 cccctttcac cttcgcaata ctcgtccaca cggcaaggta aataatggga gaaaccaacc
     2881 aatacagtag gtacatcaac aagttggcgc ggtattgcca ttgttccgcc cagttcactt
     2941 gccagagtcg tttgaaaatt agaaacataa aatactccaa tgctatgcgt tgaacgcctg
     3001 ctcgatcaca tcctcgatcg gtggatcagc gatggtgata tcgtggatgg gcagatctgc
     3061 caacagccgc gacgtgaccc gcgtcacttc ctccgccttg acctggatgt agcgcttctc
     3121 ggcatcttct tcgttctgaa ccacttcccc atacttgctc aggtcatgcg aatcggcatc
     3181 ggcaagcatc acaccgatca acttgaaagg tgcgatcttc ttcgagaggt cgttgatgcc
     3241 gccatcatat ttcaactgtc cgtggtgaat gatgatgatg cgctcacaaa gggctgtcac
     3301 atccgccatg tagtgactgg tcagcaagat ggtcgcgccg gtgcgcttgt tgtactcgcg
     3361 caaaaactcg cggatgcgtc cctgcccggt gatatccaag ccaatggtcg gttcatcgag
     3421 gaacaaaact ttcgggcggt gtaaaagtcc cgccgcgatc tcgcacttca tgcgttcacc
     3481 caaagacaag ttgcgaaccg gcttcttcat caacggttcg agttcaagca attcatctaa
     3541 ttctttgtat gtttctttat attcatcatc ggggatacga taaatggctt ggttgagcag
     3601 gaacgaatcc gctgcaggga tgtcccatga caggcggttg cgttgtccca tcaccaaggt
     3661 catcgagcgc agatattcag gcttgagctc ccaaggcgtg aatcccaaca cattggcact
     3721 tccgcctgtc ggatgcagca accccgaaag catcttcaag gttgtagttt tgcccgcacc
     3781 gttcggtccg agaaagccaa ccacctcgcc ttgctcaatg gtgaagtcca cttgttgaac
     3841 ggctttcacc tctttatatt tgcgtttgaa aaagctacga acagccgcgc caaatccacc
     3901 ttcgcgctcc ggcacctgat acgttttact taaatgttga acttgaatgg tcggggacat
     3961 aaaaaatctc tcctgagaag ggagagattt tatcattttt agaacaaaag tgctagacta
     4021 aaattcagag actcgatacc aaatatccac gttgacatgg atggtcttcg agtgttgcac
     4081 atccatgccg aggcttggtt cttgctccgt gggtgcagaa cgaaatgcag gctttgccag
     4141 agcctgatag ggcaccgctg tttcttcatc atatatgttt tcgatcaagt catacacgcc
     4201 caataacttc acccccatcg tctgcgcgac cttggcggct ttgcttttgg ctttttccaa
     4261 agtcattagc aacaacgcct cacgcgtcac atcttcgtca tatttccatt ctatctgtgt
     4321 gagagacgta ttcttctgtg acgcgatcac atcgagaatt tctgcaaact tcgccaaatc
     4381 ttcacagcgg atttttaaac gataggatgc tgtcgatgat ttcaacaccg cacccgtgga
     4441 tgtggacaca ttcacacctt gaagtgtgac cgcctcttcc ttaactccag cctgcttcag
     4501 cgcttcgagc agttgattca cttccttggc tttcttcatc gcctcgttgc cgctgaccaa
     4561 tgaagagcct ttcaccacca catgcagatc cgcatgggtg gcaaatattt cttctttatg
     4621 agatgcagag actttgatgg tgtcgggttt gtgttccata tactattttc atcctctgct
     4681 ttttaagcag caaaaaacga actccacgaa gcaataacag ccaacagcac agaaacatgg
     4741 atcagtatat gctgcacatc aggaattacg ggattcctat taaaacgcgg caggatgcgt
     4801 acattgcgcg aataatgttg ctcataaatt tcaggcgtta tttctccctg atggatatta
     4861 aagaaataag caatccacca gttgcgaatg gcagacaaca aaacaactga ataccaaatt
     4921 gcgccgacca agatgaccca cttccacacc agaaggttgg caatcgcaaa ccctattccc
     4981 aacaaaccat gaatgaacgt acttggaagt ctctctttga cactcattcc atcaatgttg
     5041 cggttcaaag g
//
LOCUS       DAOQFT010000114         5035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8667_length_5035_cov_7.593574, whole genome shotgun sequence.
ACCESSION   DAOQFT010000114 DAOQFT010000000
VERSION     DAOQFT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8667_length_5035_cov_7.593574"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PLR93_04230"
     CDS             complement(<1..566)
                     /locus_tag="PLR93_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNH26200.1"
                     /translation="MAAKQLVFSEDARRKLKNGMNVVANAVSATLGPKGRNVAIDRKF
                     GSPTITHDGVSVAKEIELEDPFENMGAQLLKEAAQKTNDIAGDGTTTSTVLAHAIVNE
                     GLKALEAGYNPMLLKRGIELATETIVAELKKNSTKIDTKEEIASVATNSAADEEVGQL
                     IADVMDKVGKDGVITVEESKTMQFETEFV"
     gene            complement(602..904)
                     /gene="groES"
                     /locus_tag="PLR93_04235"
     CDS             complement(602..904)
                     /gene="groES"
                     /locus_tag="PLR93_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNH26201.1"
                     /translation="MAKNNVSFKPLGSRVLIETIEQEEITASGIILPETAKEKPQQGK
                     VLAAGPGDRNDKGERVAMDVKTGDVVLFAKYSGTEVKMDGKKLLILRESDILGIVG"
     gene            complement(1154..2578)
                     /locus_tag="PLR93_04240"
     CDS             complement(1154..2578)
                     /locus_tag="PLR93_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH26202.1"
                     /translation="MNIYNKRSLFRRRDESSVYRMFFVVIFILGGIWLIRSVQQGDVK
                     PLFLPTPTPTRFATSYTMEGDANFTAGQLNAAIEAYQEAATIDPSNADVWAQLSRVQT
                     YSTALIVQREDLLKRLDEAIVSAEKAIEVNPDSSNAHAVYAFALDWKASYTSDSRERQ
                     TLLQDAEQHATRAILLDNTNVLAQAFYAEILVDQQKWTQAQQFIDQALAADPTLMDVH
                     RVHAYVLESLGEYALAIEAYDKAIAIAPNMTFLYLRAGAGYRRLAFESANDEVQRQLF
                     EKSLEYFAQTARINSQLGVKDPAPYISIAKTYSQMGEFFIAIRNIQKAIEFEPANAVL
                     YGELGVLYHKNRNYETGILALGCAINGCTGPESCDGRGGCGPNDVEAEVKPLALDGST
                     VYYYDILASELAALSTPKNNKCPQALEVAAIIENSEYISDPNIATDMAVVRTICSFSP
                     ETTNTGSEPTPTAESATPEVTPTP"
     gene            complement(2575..3831)
                     /locus_tag="PLR93_04245"
     CDS             complement(2575..3831)
                     /locus_tag="PLR93_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH26203.1"
                     /translation="MNKRRTRPNWFRLALLSLLVLGAAYVNRFVVTNVDPLGVPSPTP
                     TISAETLSDQGDELFKQGKLVPAIDAYNQAIVSQPDNPALHITLARTLAFAGKYKEAQ
                     TAAEDALLLNPNNSMAHAVRAWALNFQGQYAEAESSITRALELDPNNALAHAYYVEIL
                     VDSYYSGLGAFDGIEKAIEESKFAQALAPDLLETHRARGYILEATGNYEEAIREYDQA
                     ISINPNISDLYLATGRNYRILGIYDKAVDAFTRADALNPEDPNPDLLLSRTYATIGEY
                     GKAMQFAESAVANNPADTNLRGNYGVMLYRNAYWEDAIRELSYVVKGGLSEDGHEMEV
                     INLVPDAPRIAEYYFTYGLALARTDNCGEALQISQLIISRVPADELAVNNANEIVNNC
                     TENLNAPPATATEEPVATEVDATPTP"
     gene            complement(4037..>5035)
                     /locus_tag="PLR93_04250"
     CDS             complement(4037..>5035)
                     /locus_tag="PLR93_04250"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNH26204.1"
                     /translation="CQTYYWRVAARNGDTIGSYSPTRSFVADTTGTCGGGGGGGTPPD
                     SASISGIVWHDICSIPDGPLPDPLSAGCVEDGAGSARANGIREEGEPGIAGVQVDLYY
                     GDCDFAGASDASVLTDADGRYIFDGLTSFGNFCVAVNPSTSPNDAVLLPGEWTYPDLI
                     TPDGVAYQTFGGIESGVHVDIIDFGWDYQFLPAFNDPTPTPVAGNLTPNIPNFVDDSG
                     FNTPLPQFVFDKNAFCRKGPNINYSDVTAISAGDTVQIQGISSDRLWYHIFWAKFNVN
                     CWVAGSTGHVVGDVQNIPVMTPPPTVVPSSVPSSVPSNNNKNNTPTPVPSVTPVKP"
BASE COUNT         1303 a         1255 c         1160 g         1317 t
ORIGIN      
        1 acgaattcgg tttcgaactg catcgtcttg gattcttcaa cggtgatgac gccgtcttta
       61 ccgaccttgt ccatcacgtc ggcgatgagc tgaccgactt cttcgtctgc cgcagagttg
      121 gtggcgacag aagcaatttc ttctttggta tcgatcttgg tggagttctt cttgagttcg
      181 gcaacgatgg tctcggtggc gagttcaatg ccgcgcttga ggagcatggg gttgtaacca
      241 gcttcaagag ccttcaaacc ttcgttcacg atcgcgtgag cgagaacagt ggaggtagtg
      301 gtaccgtcac cggcaatatc attggtcttc tgggcggctt ccttgagaag ctgagcaccc
      361 atattttcaa acggatcttc gagttcgatt tccttggcca cagacacgcc atcgtgggtg
      421 atggtggggg aaccaaactt gcgatcgatg gctacattgc gtcccttagg accaagagta
      481 gcggataccg catttgcaac gacattcatg ccgttcttta gcttgcggcg ggcgtcttcg
      541 gaaaatacca gttgtttagc tgccataatt cacttcctta tatctagaaa tttgtttggg
      601 attagccgac gatgccgaga atatcgctct cgcgcagaat gagcaatttc ttgccatcca
      661 tcttcacttc ggtaccagaa tacttggcga acaaaactac atcaccagtc tttacatcca
      721 tggcgacgcg ctcgcccttg tcgttgcggt caccaggacc agcagccaag accttgccct
      781 gctgcggttt ttccttcgcg gtttcgggga ggatgatccc gcttgcggtg atttcctcct
      841 gttcaatggt ctcgatcagc acgcggctgc cgaggggttt gaacgaaaca ttgttctttg
      901 ccatttgatg cctcctgtaa ggttgatact tttgattttt taggataatc acagaattag
      961 cactcaatac aactgagtgc taaagtctat cttacacagt taaaaagtca acgtcaaggg
     1021 gatatataaa aattcttaca tttctctgat gacgcttcgc ccgcctcttt ctggacccgc
     1081 tttctgcctc gcctacccga gccttatccc cgctgtaccc tggctctggc aggcgctagg
     1141 accctgtcgt caatcaaggc gtgggcgtca cctcaggggt ggctgattca gccgtcggcg
     1201 ttggttcaga gccggtgttt gtcgtctcag gagagaacga acagatcgtt cgaaccacag
     1261 ccatatccgt agcaatgttc gggtctgaaa tatattcaga gttttcgata attgccgcaa
     1321 cctctagcgc ctgagggcat ttattatttt tcggggtact caacgccgcc aactcagaag
     1381 caaggatatc gtagtaataa accgtactac catctaatgc caatggcttc acttcagctt
     1441 ccacgtcgtt gggaccacag ccaccgcgcc catcacaaga ctcaggtccc gtacaaccat
     1501 taatcgcgca ccctagggcg agaataccgg tttcatagtt acggttcttg tgatacaaca
     1561 cgcccaactc gccatacaaa acggcattag caggctcaaa ctcaattgcc ttttggatat
     1621 tacgaatggc aatgaagaac tcacccattt gcgaataggt ttttgcaatg gagatatagg
     1681 gcgctgggtc tttcacaccc aactgcgaat tgatacgagc cgtctgtgca aaatattcaa
     1741 gcgacttctc aaacaactgt ctttgcactt cgtcattagc gctttcaaaa gcaaggcgcc
     1801 gataccctgc ccccgcccgc aggtaaagaa acgtcatatt tggcgcaatc gcgattgctt
     1861 tatcatatgc ttcaatcgcg agcgcatatt cgcccaaaga ttccaacaca taggcatgca
     1921 cacggtgcac atccatcaat gtggggtcag ctgccaatgc ctgatcgatg aactgttgtg
     1981 cctgtgtcca tttttgctga tcgacaagga tttctgcata gaaagcctgt gccaacacat
     2041 tcgtgttatc aaggaggatg gcacgtgtag cgtgctgttc tgcatcttga agcaacgtct
     2101 gtctctcacg gctatctgat gtataagaag ctttccaatc tagtgcaaat gcatataccg
     2161 catgcgcatt gctactatct gggttcacct caatcgcttt ttctgctgaa acaatcgctt
     2221 cgtccaaccg ttttaacaag tcttcgcgtt gaacaatcaa tgcagtagag tatgtttgca
     2281 cacgcgaaag ctgtgcccaa acatcagcat tacttgggtc gattgtggca gcttcttgat
     2341 aagcttcaat ggcagcattt aactgccctg ccgtaaagtt tgcatcgcct tccatggtgt
     2401 aggaagtagc gaaacgggtg ggagttggcg tgggcaaaaa gagcggcttt acatcgccct
     2461 gctgcaccga gcggattagc caaattccgc cgagaatgaa gatgacgaca aagaacatgc
     2521 gataaacact agactcatcg cgtcgcctaa ataaactacg tttgttataa atattcatgg
     2581 ggttggggta gcatctactt cagtagctac aggctcttct gtcgctgtag caggaggagc
     2641 attcaagttt tcggtgcaat tattgacgat ttcgttggca ttgttcacag caagttcatc
     2701 tgctggaaca cgcgaaatga ttaactgcga aatttgtaat gcctcgccac agttatctgt
     2761 acgagcaagc gccaaaccat aagtgaaata atactcggcg atacgcggcg catctggcac
     2821 aaggttgatc acttccattt cgtgaccatc ttccgaaagt ccacccttga ccacatatga
     2881 aagttcacga atagcatctt cccaataggc attacgatac agcatcacgc cataatttcc
     2941 gcgcaagttg gtatctgcag ggttattcgc cactgctgat tcagcgaatt gcatggcttt
     3001 accgtattca ccgatcgtgg cataggtgcg ggagagtaac aggtccggat tggggtcttc
     3061 agggttcaat gcatcggcac gcgtaaaagc atccactgcc ttgtcgtaaa ttccgagaat
     3121 acgataattt cttcctgtag caaggtagag gtccgaaata tttgggttga tagaaatcgc
     3181 ctgatcatat tcacggatag cttcttcgta atttcccgtc gcctccagaa tatatccacg
     3241 agcacgatgg gtttcaagca aatcaggcgc aagtgcctgc gcaaacttgg attcttcaat
     3301 cgctttctct atgccatcaa aggcgcccaa accactgtaa tatgaatcga ccaaaatctc
     3361 tacataatag gcatgcgcca aggcattatt agggtcaagc tctaaagcac gggtaatact
     3421 agattctgct tcagcgtatt gtccttgaaa attcagtgcc cacgcacgca cggcatgtgc
     3481 cattgaatta ttagggttga gtaataacgc atcttctgca gcagtttgag cttctttata
     3541 tttgccagca aatgccaaag tccgcgccaa ggtgatgtga agcgcagggt tatctggctg
     3601 agatacgatc gcctggttat atgcatcaat cgcagggaca agtttgccct gcttgaaaag
     3661 ttcatcccct tgatcgctca atgtttcggc tgaaatagtc ggcgttggag aaggtacgcc
     3721 taagggatca acattcgtca ccacaaaacg gttgacatag gctgcaccaa gcaccaacag
     3781 gctaagcaat gcaaggcgga accagtttgg gcgggtccgc cttttgttca tactccactt
     3841 acttcctcgt aaatacataa aatcatatta ccatccggca gaaagcaacg tcaagcaggg
     3901 gttttcgctt ctaatttgct tcttagcaaa aatcacattt ctgccatgtt tttgacggaa
     3961 aatatgcatt ctcaaaataa acggacctgt gaagttggct tcacaggtcc gtttattaat
     4021 actaagaaat ttttgcttat ggctttaccg gggtcacact tggcaccggt gtgggggtat
     4081 tattcttgtt gttattagac ggaacagacg aaggcacgct ggaaggcacc acagtaggcg
     4141 gtggagtcat aaccggaata ttctgtacgt caccgaccac atgccctgta gagcctgcca
     4201 cccagcagtt cacattaaac ttcgcccaga aaatgtgata ccaaagtcta tcggagctga
     4261 tgccttgaat ctgcacagta tcaccagcag aaatagcagt gacatcgcta tagttaatat
     4321 ttggtccttt tctacagaaa gcatttttat cgaaaacaaa ttgtggcagt ggggtattaa
     4381 aaccactatc atctacaaaa ttgggaatgt ttggcgttag atttcctgca actggagtag
     4441 gcgttgggtc attaaaggca ggcaggaatt gataatccca accaaaatca ataatatcca
     4501 catgcacacc agactctatt ccgccaaagg tttgatatgc tacaccgtcc ggcgtaatca
     4561 gatcaggata agtccactcg ccagggagta ggacagcatc gttaggcgac gtgctcgggt
     4621 taactgcaac acaaaaattt ccaaatgaag tgagaccatc aaatatatat cttccatctg
     4681 catctgtcag aacgcttgca tcacttgctc cagcaaaatc gcaatcgcca taatataaat
     4741 cgacttgtac ccctgcaatg ccaggttcgc cttcttcgcg gattccattc gcacgagcag
     4801 aaccagctcc atcttccaca cacccagctg aaagcgggtc aggaagcggt ccatcaggta
     4861 tggaacaaat atcatgccaa acaattccgc tgatagaagc tgagtcaggt ggggttccgc
     4921 caccaccgcc accaccgcaa gtaccagtgg tatcagcgac aaaactacgc gttggcgaat
     4981 acgagccaat agtatcgccg tttcttgcgg caacacgcca gtaataggtt tgaca
//
LOCUS       DAOQFT010000115         5019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8700_length_5019_cov_6.006648, whole genome shotgun sequence.
ACCESSION   DAOQFT010000115 DAOQFT010000000
VERSION     DAOQFT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8700_length_5019_cov_6.006648"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLR93_04255"
     CDS             complement(<1..590)
                     /locus_tag="PLR93_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH26205.1"
                     /translation="MTKIYTLVNQKGGVGKTTSTINLAAYLAKLGQRVLVVDLDPQAN
                     ATSCLGVDKLLVEGSTYDALLGEDDIFPFILLNERLQLSLLPSSPSLAGAEVELVDEL
                     ARESRLRKALLKVASRYDYVLIDCPPSLGLLTVNGLMAAIDGVVVPVQCEYLALEGLG
                     QLTETIERVRAALFPDLKVRGVILTMFDNRTNLSADV"
     gene            complement(587..1429)
                     /locus_tag="PLR93_04260"
     CDS             complement(587..1429)
                     /locus_tag="PLR93_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH26206.1"
                     /translation="MSDLRPVDYLLIGHVAVDLTPSGKQLGGTVSYAALTAKAMGLQV
                     GIVTSAGEDAPLNLLDGVQIFNLPTEQSTIFENVKTEHGRKQTLHHQATKISFEHVPQ
                     IWRDAAIVHLAPIAQEVDPALVGQFPASWVGVTPQGWMRGWDEKGSVFATAWENSEQV
                     LGQVGGVVMSLEDINHDLELGEWLAHHARLLCLTEGEEGAVLHWHGDRRRYRPIKVEE
                     VDATGAGDIFAAGFFSRLHQTKDPWEAARFATHLAAYSVTRVGLQGIPTHDEIEHCKM
                     EVLS"
     gene            complement(1540..2403)
                     /gene="jag"
                     /locus_tag="PLR93_04265"
     CDS             complement(1540..2403)
                     /gene="jag"
                     /locus_tag="PLR93_04265"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNH26207.1"
                     /translation="MSERTTLEIIAPTVEEAIQQGLDQLGLTADAVSVEVLDSGSKGL
                     FGLGGRQVRVRISVNPPPMPEIPAARPEPKPAPAKKAESKPKAKEPKEPKPRAEKKQA
                     EPKTEAKPEPKPEPKPQKPAPQRSTINVENDAVLNTAEEVVSKLIYHMGMTAQVSAHY
                     DESDSADNRTIQVDVRGEDLSALIGRRAETLASFQHVASLIVGKQTQQWVQVIIDVEG
                     YRARREKQIRQLANRMADQVTKTGRKVTMEPMPSSERRVVHIELRGHPAVKTESTGEE
                     PYRKVVILPKE"
     gene            complement(2413..3231)
                     /locus_tag="PLR93_04270"
     CDS             complement(2413..3231)
                     /locus_tag="PLR93_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNH26208.1"
                     /translation="MWESFIIQPMVNLLLWIYDILGHGPQMFGLAIILFTIVIRLITW
                     PLNASQVKGAQAMQELNSDKEWQDIQKKYAKDREKLAQEQMRIYKERGINPFASCLPT
                     LIQFPIIIGLYQSIIRAMAATPLDMLNLARTIYPFQHVSNIIPLNSTFLWMNLGRPEG
                     IPLPFDVSFLPNGFPLLALVVAITTYVQTKLTMPTTPPNPNDQASAQTAAMSSSMSIY
                     MPLMLGWFALTFASGISIYFITSNTLGIIQYAVTGRANWSNLLPGGNKSTPTKK"
     gene            complement(3244..4317)
                     /locus_tag="PLR93_04275"
     CDS             complement(3244..4317)
                     /locus_tag="PLR93_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH26209.1"
                     /translation="MKKINLILLLVLGAVLLSACTGGRASIVNTWAGLAADGERAYLS
                     SGSHVYAVDVQTGKEVWRYPAETDSNIIFYANPVLTNDGQLLVGSEGNNHELVSINPE
                     TGKDNWAKPFSVAKGKWVASPLVFNDIVYAPNSDGFLYILDLNGETSADPIELGGSLW
                     STPVTDGTYIYVTSLDHHLHIVDPASNSIVSTIDLGGASPSSPVVGEGGVYVGSFASN
                     IEFVTSNGDHEVLATATNWIWGTPALDNGTLYYADLDGVLYSLDVNSGKQNWTDVKPD
                     GPVIANPVVVGDAIYFVTEAGTFLALDRDAKTIWEKEVGGKIYTTPVVSGDMVLVAPY
                     KAEFALAAYDAEGKQAWTFTPEK"
     gene            complement(4381..4692)
                     /gene="yidD"
                     /locus_tag="PLR93_04280"
     CDS             complement(4381..4692)
                     /gene="yidD"
                     /locus_tag="PLR93_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNH26210.1"
                     /translation="MNPDTEEQHEHFVLHDYSHEPRLRDMPRTLGNIPRIVVLSIIRL
                     YQMAISPGLPANTCRFYPSCSHYGYQAVYKHGAFKGSFMAAWRVLRCNPFNPGGYDPV
                     P"
     gene            complement(4682..>5019)
                     /gene="rnpA"
                     /locus_tag="PLR93_04285"
     CDS             complement(4682..>5019)
                     /gene="rnpA"
                     /locus_tag="PLR93_04285"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNH26211.1"
                     /translation="KRVRRTGKSYAHPLVVLVTQASETKEHLKVGIAAGKTTGTAVSR
                     NRAKRLLREAMRDLIPSITLRLAQENASGWDLILIARPGLVTATLADTRSALTNLLKR
                     AKLLPVDES"
BASE COUNT         1235 a         1305 c         1282 g         1197 t
ORIGIN      
        1 acatcggcag aaagattggt gcggttgtcg aacattgtca gaatcacgcc gcgcactttg
       61 aggtccggga aaagagccgc gcgcacgcgt tcaatcgtct cggtcaactg acctaaacct
      121 tcaagcgcaa gatattcgca ttgcaccggt accaccacgc catcaattgc tgccatcaag
      181 ccattcacgg tcagcagtcc caaagaaggc gggcaatcaa tcaacacata atcataacgg
      241 cttgccacct tcaacaacgc cttgcgcaaa cgagactcgc gtgcaagctc atccaccagt
      301 tctacttcgg cgccagccaa agaaggcgaa gaaggcaaca gcgacaactg caaccgctca
      361 ttcaataaga taaacggaaa aatatcatcc tcgccgagca gcgcatcata agtactgcct
      421 tctacgagaa gtttatccac gcccaaacaa gaggttgcgt ttgcctgcgg gtcgaggtct
      481 accaccaaca cacgttgccc cagcttcgcc aaatacgccg ctaggttgat cgtggatgtg
      541 gtcttcccca caccgccttt ttgattcacc agagtataga tcttcgtcaa gacaaaacct
      601 ccatcttgca atgttcaatt tcatcatgag tgggaatgcc ctgcaaaccc acacgtgtta
      661 cagaatatgc cgccaaatga gtcgcaaaac gtgctgcttc ccacgggtct tttgtttgat
      721 gtaaccgcga aaaaaatcca gccgcaaaaa tatcgcctgc ccccgtcgca tccacctctt
      781 ccactttgat gggacgatac cgtctgcggt cgccatgcca atgcaacacc gcgccctctt
      841 ccccttccgt caaacacagc agacgggcgt gatgcgccag ccattcaccg agttccaaat
      901 catggttgat gtcctcaagc gacatcacca cgcctcccac ctgaccgaga acctgttcgc
      961 tgttttccca agccgtagcg aacaccgaac ctttttcgtc ccagccacgc atccagccct
     1021 gcggcgtgac tcccacccac gaggctggga attgccccac caaagccggg tccacttcct
     1081 gtgcaatcgg cgcgaggtgg acaatcgccg catctcgcca gatctgtggg acatgctcaa
     1141 acgaaatctt cgtcgcttga tgatgcaagg tttgtttgcg tccgtgctcg gttttaacgt
     1201 tctcaaaaat cgtgctttgt tcggttggga ggttgaatat ctgaacccca tctaaaaggt
     1261 ttaagggagc atcctccccc gctgaggtga caatgccaac ctgcaagccc atcgcctttg
     1321 ccgtcaacgc cgcgtatgaa accgtcccac cgagttgttt gcccgatgga gttaaatcga
     1381 cagccacatg accaataaga agatagtcca ctgggcgaag gtcagacata ggtgaattat
     1441 accgtagtga aatctttcct ctttcttctt tcttgaaaga agaaacaaaa agaccgtaga
     1501 ttttcaccta cggtctttta tctatcagta aatgtcgaac tattctttcg gcagaatgac
     1561 cactttgcga tagggttctt cgccggtgct ctcggtcttc acggcaggat gtccgcgcaa
     1621 ttcgatgtga accacacggc gctcactaga aggcatcggc tccatggtca ccttgcgtcc
     1681 ggtcttggtc acctggtcag ccatgcggtt ggcaagctgg cgaatctgct tttcacggcg
     1741 agcgcggtag ccttccacgt cgatgatgac ctgtacccat tgctgggtct gcttgccaac
     1801 gatcaacgat gcgacgtgtt ggaaggaagc caaggtttcg gcgcggcgtc cgatcagggc
     1861 gctaaggtct tcaccacgga cgtccacttg aatggtgcgg ttatcggcag agtctgattc
     1921 atcgtaatga gcagagacct gtgcagtcat gcccatgtga tagatcagct tggataccac
     1981 ttcttccgcc gtattcaaca ccgcgtcatt ctcaacatta atcgttgagc gttgtggagc
     2041 aggcttttgc ggcttcggtt caggtttcgg ttcaggcttg gcttctgttt tgggttctgc
     2101 ctgtttcttt tcagctcgtg gcttgggctc ttttggctct ttcgctttcg gcttggactc
     2161 agcttttttc gctggagcag gcttcggctc aggtcgagcc gcggggattt ccggcatggg
     2221 cggagggttc acagaaatgc gaacacgcac ttgacgtcca ccgagtccaa ataatccttt
     2281 ggagccggag tctagaactt ctacagaaac cgcatccgcc gtgagcccga gttggtcgag
     2341 cccttgctgg atggcttctt caacagttgg agcgataatt tctaacgtag tccgctcact
     2401 catggtgcct ccctatttct ttgtaggtgt agacttgttt ccaccgggca acaaattgct
     2461 ccagttggct cgacccgtta ccgcatactg aatgatgccc aacgtattgc tggtaataaa
     2521 gtagatcgaa atgcctgagg caaatgtaag cgcaaaccaa cccaacatga gcggcatata
     2581 aatggacatg ctgctgctca tggcagcagt ttgtgcagaa gcctggtcat tggggttggg
     2641 cggtgtagta ggcatggtca gtttggtttg aacataggta gtaattgcca caaccaacgc
     2701 caagagcggg aaaccgttcg ggaggaagga cacatcgaac ggaagaggaa tcccttcggg
     2761 gcgaccgagg ttcatccaca agaatgtact gttaagcggg atgatgttcg aaacatgctg
     2821 gaaagggtaa atggtgcgcg ccaaattgag catgtcgagc ggggtggccg ccatagcgcg
     2881 gatgatgctc tgatacaagc cgatgatgat cggaaattgg atcaaggtcg gcaaacagga
     2941 agcgaaagga ttaatgccgc gttctttgta aatacgcatt tgttcctgcg ccagcttttc
     3001 gcggtctttg gcatacttct tttgaatgtc ttgccattcc ttgtcgctgt ttaattcctg
     3061 catggcttgg gcgcccttta cctgtgaggc attgaggggc caagtaatga gcctgatcac
     3121 gatggtgaat aaaatgatgg caagaccaaa catttgcggt ccatgaccta aaatgtcata
     3181 gatccacaaa agcaggttaa ccatcggttg gatgataaat gattcccaca tgggctaggt
     3241 tccttatttc tcgggggtaa acgtccaggc ttgtttgcct tcagcgtcat aagccgctaa
     3301 tgcaaattct gccttatacg gcgcaacgag gaccatgtcg ccggaaacaa caggagtggt
     3361 atagattttg ccgcccactt ctttttccca aatggtcttt gcgtcacgat ccaaagcgag
     3421 gaaagtaccc gcctcagtta cgaaataaat ggcatcgcca acaactaccg ggttcgctat
     3481 cacaggacca tctggcttga catcggtcca gttctgtttg ccagaattca catcgaggga
     3541 gtaaagaaca ccgtcgaggt cggcatagta caaagtgccg ttatcgagtg cgggagttcc
     3601 ccagatccaa ttcgtagcag ttgccaaaac ttcatggtcg ccattggaag tgacaaactc
     3661 aatgttcgaa gcaaatgagc ctacatatac tccaccctca cccacaacgg ggctgctggg
     3721 agaagcacca ccaaggtcaa tggtgctgac gatggaattg cttgcaggat ccacaatgtg
     3781 aaggtgatga tctaacgaag tgacgtaaat gtaagtacca tctgtcacgg gcgtggacca
     3841 aagtgagccg cccaattcga tggggtcggc agaagtctca ccgttcaagt cgaggatgta
     3901 gagaaagccg tcggaattgg gagcgtagac gatatcgttg aaaacaagcg gagatgccac
     3961 ccacttccct ttagcaacag agaagggttt tgcccagtta tctttgccag tttcggggtt
     4021 gatgctcacc agttcgtgat tgtttccttc acttcccacc aacaattgac catcgttggt
     4081 aagtactgga tttgcataaa agatgatatt gctatcggtt tctgcggggt aacgccaaac
     4141 ttctttgccg gtctgcacgt ccacagcata gacgtgggag ccagaagaaa gataagcacg
     4201 ttcgccgtcg gcggcaagac ccgcccatgt gttaacaatg gaggcgcgtc cgccggtgca
     4261 ggcgctcaac aaaacagcac ccagcacgag caagagtata agatttatct ttttcatatt
     4321 taaattggat ttactcctaa aaacggcaaa ggcaggacct ttgccgtgaa agatgcacta
     4381 ttacgggacg gggtcgtacc cgccggggtt aaagggattg caacgcaaaa cccgccaagc
     4441 tgccatgaaa gagcctttga atgcaccatg cttatatacg gcttgatacc cataatggga
     4501 gcacgatggg taaaagcggc aggtgttggc aggcaagccc ggtgagatag ccatttgata
     4561 taatcgaatt atagacaaga ccacaatacg cggaatattc ccaagcgtac gtggcatatc
     4621 gcgcagccgc ggttcatggg aataatcatg caatacaaaa tgttcgtgtt gttcttcggt
     4681 atcaggattc atcgacaggg agaagcttgg cgcgtttgag gaggttggtc aacgcggagc
     4741 gggtatctgc cagggttgca gtaacaagcc cgggtctggc aatcaggatc aagtcccagc
     4801 cagaggcgtt ctcttgagcg agtcgcaggg tgatggaagg gatcaagtcc cgcatcgctt
     4861 cacgcaagag tcgttttgcg cgattccgac tgacagcggt gccggtcgtt ttccctgctg
     4921 caatgccaac ttttaaatgt tctttggttt cactggcttg agttactaac acaacaagcg
     4981 gatgggcata ggacttgcct gttcgccgca cccgcttga
//
LOCUS       DAOQFT010000116         4983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8791_length_4983_cov_8.157062, whole genome shotgun sequence.
ACCESSION   DAOQFT010000116 DAOQFT010000000
VERSION     DAOQFT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8791_length_4983_cov_8.157062"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLR93_04290"
     CDS             <1..198
                     /locus_tag="PLR93_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26212.1"
                     /translation="RWTTKARAYEKETGQARPLYFVDANGDPVSLRPGNTWFIVATPY
                     SYFSSEDGVWNFRFVPPEGVK"
     gene            211..885
                     /locus_tag="PLR93_04295"
     CDS             211..885
                     /locus_tag="PLR93_04295"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNH26213.1"
                     /translation="MHPYVRSIKTLFEANANPVNAAPMKKYMRDQFEYLGIKSPQVKS
                     LFSGYVKQNGLPPIEDLSVIVSELWNLPQREFQYLAINLLDKMEKKLPEDFIATLEHL
                     ITHKSWWDTVDLIAGHSVGVMFKRYPKVKAQYLKKWRKSENIWLRRTTLLFQLGYKKE
                     TDFGLMCELIQENLGSDEFFINKAIGWALRQYAWTNPSAVKKFVKATKELSPLSRKEA
                     LKNIGA"
     gene            complement(882..1295)
                     /locus_tag="PLR93_04300"
     CDS             complement(882..1295)
                     /locus_tag="PLR93_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNH26214.1"
                     /translation="MTEQSLYELLGGEEGVRTLVDRFYDLMDSSPEAKDVRALHAKSL
                     KQAREKLFMFLSGWSGGPPLYIEKFGHPRLRMRHMPFVIGTRERDQWLWCMNRAVDAG
                     NYHSAVVEHLKQRFSEIADFMRNQDETEQGPLLQL"
     gene            complement(1360..4239)
                     /locus_tag="PLR93_04305"
     CDS             complement(1360..4239)
                     /locus_tag="PLR93_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNH26215.1"
                     /translation="MSDVFISYSRRDKVFTQKLYEALRDSGREIWADWDSIPAASDWF
                     AEIKDGVEKANTIVFVLSPEWLKSNECRKEYDHAVAMGKRLLPILYQVVDPKEVPSEL
                     AKINWVYMRDTDDFGQAFQTLSAAMDTDLEWVKTHTRIQIRAIEWDKKSRDHSFLLRG
                     NDLTDGEQFIAQGAQKSPEPTVLHGEYVLASRKDATRRQRITLAGVTVALVVSVALGI
                     VAFFQRQAAVAARAEAVVERDRAVAAEKIAEDERDRADINAEVAYSRELAAASLNSLN
                     HDPELSLLLGLQSVAITDKTGMPIQPMSEDALRRSVQTSRIRKTWLAHSGSVLDIAIS
                     PDGTKIASASDDLTVKIWDLSGKLLLTIPDFSEEVDSVVFSPDGSQILTSSGQEAVAW
                     DAVTGEKLVEYKGHVSYVVQAVYSPDGKFIATASSDATAIMWDALSGESLVVLGGHET
                     ELSDIAYSPDGEMIASTDYNGAMYVWSTSSKSLLYMLSEGEGSVILDVEFSPDGKYIL
                     ISDNYFQTIVLVAQTGKVISAQNVHTDFVTNAVVSPDGAFMASASTDNTVVVSSITAS
                     AVLFGQSISADPDYIDISKVMFRFTDSTDINTMIYTPDGKYLITGNDEGQIKIWDANP
                     ISDYEVSGFRPHTARIDGMEFSPDGEHYVTVARDGKAFIWSLTDQSSIELVGHQNVIQ
                     DVAYSSDGMQIITGGFDGHAIIWDAETGDKLLTLSKDDQLVTGVNFSPDGKLAVGSLG
                     DNTVVVWDAVSGDEVRVLQSKDMYAYINELAFSPDGKTLAVSSDAGFVVLWDVATWNQ
                     IDVLAGHTDWVKGLDYSSDGTHMVTASYDTTAIIWDVATRTPVLKLEGHSQGVNDVTY
                     SPDDTLVATASVDGTAKLWNANTGELLVTYQTSADQILSVAFSADGKYLATGSETGVV
                     RIYYVYAEDALELAKSRITRTFTEEECLKDLHEEACPANLPQPQQ"
     gene            4407..>4983
                     /locus_tag="PLR93_04310"
     CDS             4407..>4983
                     /locus_tag="PLR93_04310"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNH26216.1"
                     /translation="MSKENEAVILSAARTPIGKFQGALSGIPAPKLGAIAVEEAVKRA
                     GIDPSEVEEVFMGNVVQAGNGQAPARQAAIYGGIPATVSATAINKVCGSGLKAAMMSA
                     QAIRAGDAELFIAGGFESMSRAPYLVSGRMGELKFGNSQMTDALLNDGLWDPFENWGM
                     GNAAEFIADEYEVTRAAMDEFALRSHLKAVEA"
BASE COUNT         1385 a         1245 c         1069 g         1284 t
ORIGIN      
        1 cgctggacga ccaaagcgcg tgcctatgaa aaagaaaccg ggcaagcccg tccgttatat
       61 tttgtagatg caaacggcga cccagtctca ctcagacctg gaaatacgtg gtttattgtt
      121 gctaccccat attcatactt ttcatctgaa gatggggttt ggaattttcg cttcgtgccg
      181 ccagaaggcg taaagtagaa agaggataat atgcatccct atgtacgttc aataaaaaca
      241 ttatttgagg caaatgccaa ccccgtcaat gccgcgccga tgaagaagta catgcgtgac
      301 caatttgagt acttgggaat aaaaagcccg caagtgaaga gcttgttttc cgggtacgtc
      361 aagcagaatg gtttgccgcc catagaagat ttaagcgtca tcgtatccga attgtggaat
      421 ctccctcagc gtgaatttca gtatcttgcc atcaacctgc tcgataaaat ggaaaagaaa
      481 ctacctgaag atttcattgc cacccttgaa cacctcatca cacacaaatc ctggtgggat
      541 acggttgatc tcattgcggg gcacagtgtt ggtgtaatgt ttaagcgcta ccccaaggtg
      601 aaggcacagt atttgaaaaa gtggcgcaaa tctgagaaca tctggctcag acgcaccact
      661 ctgttgtttc aactgggcta taaaaaggaa acagactttg gactaatgtg cgagctaatt
      721 caagagaacc ttggctctga tgagttcttc atcaacaaag ccatcggttg ggcattacgc
      781 caatatgcgt ggaccaatcc cagcgctgtg aaaaaattcg tgaaagccac aaaggaatta
      841 agtccactta gccgaaaaga agcgctgaaa aatattggcg cttaaagctg caaaagggga
      901 ccttgttcag tttcatcctg attgcgcatg aagtctgcaa tttcggagaa gcgctgtttg
      961 agatgctcaa ccacagctga gtgataattc cctgcgtcca cggcgcgatt catacaccat
     1021 aaccactggt cacgttcccg cgtaccaatg acaaacggca tgtgccgcat ccgtaaacga
     1081 ggatgcccaa atttctcgat atatagcgga ggtccgcctg accagccaga gagaaacata
     1141 aataacttct cgcgagcttg cttcaagctt ttggcatgca aagcacgtac atcttttgct
     1201 tccggcgaag aatccatgag gtcatagaag cggtcaacca aggtgcgcac accctcttca
     1261 ccgccgagta attcataaag ggattgttca gtcatagcgg cattgtaatc gaaagaaata
     1321 aaaaccgcct tctatctact agaaggcggt ttactttagt tactgttgtg gctgagggag
     1381 attcgccggg caagcttctt cgtgcaaatc tttcaaacat tcttcttctg taaatgtacg
     1441 cgtgatcctg cttttggcga gttcaagggc atcttcagca tatacatagt agatacgcac
     1501 cacgcccgtc tcgctgccag ttgccaaata ttttccatca gcgctaaatg ccacacttaa
     1561 gatttggtcc gccgaggttt gatatgtgac caataactcc ccagtatttg cgttccaaag
     1621 cttggcggtt ccatctacac ttgcagtagc caccagcgta tcatctgggc tgtatgtcac
     1681 atcattcact ccctgggaat gtccctccaa tttcaaaaca ggggtccgcg ttgcaacatc
     1741 ccaaataatc gccgtggtgt cataacttgc cgtaaccata tgcgtaccat cagaactgta
     1801 atccaagccc ttgacccaat cggtatgccc agccaataca tcaatttgat tccatgtagc
     1861 aacatcccat agaacaacaa atccggcatc gctacttaca gcaagagttt taccgtctgg
     1921 gctgaaagcc aattcgttga tatatgcata catatctttc gactgcaaaa cacgtacttc
     1981 gtcaccagat acagcatccc aaaccactac tgtattatca cccaatgagc ctacagccaa
     2041 tttaccatca gggctaaaat taaccccagt aaccaactga tcgtccttag aaagtgttag
     2101 taatttatca cctgtttcag catcccaaat aatggcatga ccatcaaaac cgccagtgat
     2161 tatctgcatt ccgtcagagc tataggctac atcttgaatg acgttctggt gtccgaccaa
     2221 ttcaatggac gattgatccg tcaatgacca gataaacgcc ttcccatctc gagctacagt
     2281 gacataatgt tcaccatctg ggctaaactc catgccatca atacgtgctg tatgtggacg
     2341 gaatccgctc acctcataat cacttattgg gtttgcatcc catattttta tttgcccttc
     2401 atcatttcct gtaatgaggt actttccatc aggggtatag atcatagtat taatatctgt
     2461 agaatctgta aacctgaaca tgaccttact aatatctata taatctgggt cggcagaaat
     2521 actttgtcca aacaatactg cgcttgcagt tattgagctt acaaccacgg tattgtctgt
     2581 agaggcactt gccataaatg ctccatccgg gctaaccaca gcattggtca caaagtcggt
     2641 atgaacattc tgcgctgaga ttaccttgcc agtttgggca acaagaacaa tcgtttgaaa
     2701 ataattatca gaaataagaa tgtacttacc atctgggcta aattctacat caaggataac
     2761 agaaccttcc ccttctgaaa gcatatacaa taaactcttc gaactggtgc tccaaacata
     2821 catagctccg ttatagtctg tcgaggcaat catttcgcca tcagggctat atgcgatatc
     2881 agacaattct gtttcatgcc caccgagcac taccaagctt tctccagata aagcatccca
     2941 cataattgca gttgcatctg aactagcggt ggcaataaat ttcccgtctg ggctatagac
     3001 cgcttgtaca acataggata catgaccttt atattcgacc aatttttcgc ctgtgacagc
     3061 atcccatgca actgcttcct gtccgcttga ggtcagaatc tgtgaaccat ctggactaaa
     3121 cacaacgcta tcgacttctt ctgaaaaatc agggatggtc aataataatt taccagacaa
     3181 gtcccagatc ttaactgtta gatcatcact ggcagatgca atcttagttc catcagggct
     3241 aattgcaata tcgagaactg aacctgaatg tgccaaccag gtttttcgaa tccgtgatgt
     3301 ttgcacagaa cgtctcaggg catcttcact cataggttga attggcatcc ccgttttatc
     3361 tgtaatagca acagattgca aacctaacaa cagactgagt tcagggtcat ggttcaatga
     3421 attcaaagaa gcagcggcta gctcacgaga atatgcaact tctgcattaa tgtccgcacg
     3481 atctcgttca tcttccgcaa tcttttcggc agccacagca cgatctcttt ccaccacagc
     3541 ttctgcacgc gcagcaacag ccgcctgccg ctggaaaaat gccacaatgc ccaaagcaac
     3601 cgaaacaacc agtgcaaccg ttacaccagc caaagttatc cgctgacggc gagtggcgtc
     3661 ctttcggctg gcaagcacat attcaccgtg gagaacagta ggttccggtg atttctgtgc
     3721 cccttgagca ataaattgct ctccatccgt taggtcattt ccacgcaata agaatgaatg
     3781 gtcacgactt ttcttgtccc attcaatcgc acggatttga atacgagtat gggtttttac
     3841 ccattccaag tctgtatcca tggcggcaga aagggtttgg aatgcctgac caaaatcatc
     3901 tgtatcacgc atgtagaccc agttaatctt cgccagctca gacggaactt cctttgggtc
     3961 tactacttga tataggatag gcaataatcg tttccccatt gccacggcat gatcatattc
     4021 tttgcggcat tcgttcgatt tcaaccattc tgggcttaac acaaaaacaa tcgtgtttgc
     4081 tttttccacc ccatctttga tctcagcaaa ccaatccgat gccgctggga tcgagtccca
     4141 gtccgcccaa atctcacgac cagagtctct tagtgcttca tatagttttt gagtgaatac
     4201 tttgtcgcgc cttgaataag aaataaatac atctgacatg tcgctctcct gatcctatat
     4261 atccataaga aatgtgtcga ttatatagta ggctttgtta tggaaagacc tacaagtttg
     4321 aaagcagaga atgaaagaac ttaacagaat ttctgcccta tggcggctct cgcgtataat
     4381 acgaatatca ccccacagga ggtacgatgt ccaaagaaaa tgaagcggtt atattgagcg
     4441 cagcccgcac accgatcgga aaattccaag gtgcgttgag cggtattcca gcgccgaaac
     4501 tcggtgcaat tgccgtggaa gaagcggtta aacgcgcagg catcgacccc agcgaagtgg
     4561 aagaagtttt catgggcaat gtggtgcagg ctggcaatgg tcaggcaccg gcacgacaag
     4621 cggcaattta cggcggcatc cctgcaaccg tcagtgcgac tgccattaac aaggtctgcg
     4681 gctcgggctt gaaagcggcg atgatgtctg cccaagccat tcgggcgggt gatgcggagc
     4741 tcttcatcgc cggtgggttt gagtcgatga gtcgcgctcc gtatctcgtc agcgggcgca
     4801 tgggcgagtt gaaattcggc aactcccaaa tgaccgatgc cctgctcaac gacggtctgt
     4861 gggatccgtt tgaaaattgg ggcatgggca acgccgcaga atttatcgcg gatgaatatg
     4921 aagtgactcg cgccgcgatg gatgaattcg cgcttcgctc tcacctcaaa gctgtagagg
     4981 ctc
//
LOCUS       DAOQFT010000117         4981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8795_length_4981_cov_7.548112, whole genome shotgun sequence.
ACCESSION   DAOQFT010000117 DAOQFT010000000
VERSION     DAOQFT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8795_length_4981_cov_7.548112"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..227
                     /locus_tag="PLR93_04315"
     CDS             18..227
                     /locus_tag="PLR93_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNH26217.1"
                     /translation="MAERIIGTVKWFNGTKGYGFIARDGGPDVFVHFSAIRGEGFKNL
                     QEGQKVEFTIEKGPKGLQAADVVVL"
     gene            complement(316..1068)
                     /locus_tag="PLR93_04320"
     CDS             complement(316..1068)
                     /locus_tag="PLR93_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26218.1"
                     /translation="MKRDLYFSKPLLNAAGSLGFTPDPRNGISLDSFGAFVTNPLSLR
                     PRLPTTHPALVEFPGGFLMHTGLPNPGFHATLKKYSAKWNRANLPIIVHLMADRPEET
                     QRMVRELENVENVMAVQLGFAPLLSDDIILMNLEMCLGEVPLIFALPHEQVLTLGRQL
                     VDGGASAISIASPRGALYDKNGNLITGRMYGQSLFPRALDMVRSAVMSGIPIIGSGGV
                     WTEMDVESMLIAGAIAVETDAQLWVPKEAPSL"
     gene            1079..2263
                     /locus_tag="PLR93_04325"
     CDS             1079..2263
                     /locus_tag="PLR93_04325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNH26219.1"
                     /translation="MQPLQNIRVLDLSRVLAGPYCTMVLGDLGAEVIKVEPPEGDETR
                     GWGPPFAGGESAYYLCVNRNKRGMVVNLKTEEGRDVLQKLAMQSDVVVENFRPGTLKK
                     FGLDFETLHELNPKLIYCSISGFGQTGPLKDMPGYDFMIQAMGGIMSVTGEPEGEPMK
                     VGVAVADLFAGQNAIISILAALQARTFTGEGQYIDIALFDSELGWLANVASNFLISGK
                     NPKRYGNAHANIVPYQSFQASDGWFVVAVGNDKQFESFCNVIENPELSSDERFSTNSA
                     RVQNREVLIPLLKPIFMQKTVNEWLALIGDQFPCGPINTFDQVFSMPHVKEREMLIEM
                     EHPTIGALPLVGSPLKMGGTPVSYRLPPPLMGQHTHEILKEVLEFSDEAVQKLMNDGC
                     IK"
     gene            2281..2793
                     /locus_tag="PLR93_04330"
     CDS             2281..2793
                     /locus_tag="PLR93_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26220.1"
                     /translation="MEVIYSVSPLKAFSGSFLFLAFFFVLGGFGALSAIFSRRDRAGK
                     RIALGCASAIILLAGTGGAVATFMTYTGGQTTALVHVDKKRIVERNCDNASNTCIDYV
                     IEASDGQKYYTFGLAKDTWDVVQDQACYEFTYYPAKSLFGEYLQQDNPYADMYETTSN
                     IVEIKTAQCP"
     gene            complement(2801..3373)
                     /locus_tag="PLR93_04335"
     CDS             complement(2801..3373)
                     /locus_tag="PLR93_04335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH26221.1"
                     /translation="MKLSAPEFNAMQSLPRKLGQRYFEMPLFKRMGLNLQNKDVLEIG
                     CGSGYGAYLLSTLNPKSYIGLDVMEEQVALAKKQYPQFQFILQGAEDLSVFADASKDV
                     VIIFGVLHHIPDWRKAIDEIIRVLKPGGSLFLEEPRGVDIKVFDAFFRWGHPNTDFGL
                     NALDAHLTQRGLTIETKQWTPLLTMYHIKK"
     gene            complement(3416..4726)
                     /locus_tag="PLR93_04340"
     CDS             complement(3416..4726)
                     /locus_tag="PLR93_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH26222.1"
                     /translation="MESQFFVAVIGAGPAGLFGARELANSGVRVALFNRDIKAGGLAE
                     YGIYLEKHTMKEGLRKQFRQALDMPNIDYYGNVAIGTKGDFTLDEIRALGFDAVLVAA
                     GAQGTKSLGLPGEELEGVYHAKDVVYGYNKLPPFSQRKFRYGKRAAIIGAGNVMVDIA
                     RYLINHQGVEEVTAVVRRGPAEVNFTKEEMKHLVAYLDMDEFEKELARVRPALDAIGQ
                     DPQIGRQKVLDALAKADPKVKEAKFKFDFLASPSGMLGENGLLTQLEVEENILTERDG
                     KTSAKGTGKKHTLDVETVIFAIGDQVDANFGLPTEWGEFSKTKEPKFPIEGTSYESTT
                     EGIFVGGWSRKASEGLVGYARRDGTNAAKAVMQYLQTKQPSNASPEAVAAKVKALQKP
                     IVYKEDIKRLEAAEAEEAKKRELEWFKYGSNEEMLQAMGLIETA"
BASE COUNT         1232 a         1175 c         1276 g         1298 t
ORIGIN      
        1 ttgctcagga ggcaaatatg gctgaacgta tcatcggcac ggttaagtgg ttcaacggaa
       61 ccaagggtta tggtttcatc gctcgcgatg gcggacccga cgtcttcgtc cacttttccg
      121 ctattcgcgg cgaaggcttc aagaacctgc aagaaggcca gaaggtggaa ttcaccatcg
      181 agaagggccc caaaggttta caggccgcgg atgtagtggt tctctaattc tgcaagtaat
      241 taattgaacc taatgaaaga gcttcgtcgt gagacggagc tcttttttgt taccttattg
      301 aaaaaaatta ggaggctaga gagaaggcgc ctctttcggg acccataact gggcatctgt
      361 ttcgacggca attgcccccg ctataagcat ggactcgaca tccatctccg tccacacacc
      421 gccagaacca atgatgggga ttccactcat caccgccgag cggaccatat ccaatgcccg
      481 cggaaacagc gactgaccat acattctgcc ggtgatgaga ttgccattct tgtcatacaa
      541 cgcgccgcgc ggagaggcaa tactgatggc actcgcacca ccatccacta gttgacgtcc
      601 caaggttaat acttgctcat ggggtaaggc aaaaataagc ggaacttcgc ccaagcacat
      661 ttctaaattc atcaagataa tatcgtctga taaaagtggc gcaaaaccga gttgaaccgc
      721 catcacattt tcaacatttt ccagttcgcg caccatgcgt tgagtttctt cggggcggtc
      781 tgccattaag tggacgatga tgggcaggtt ggcacgattc catttggcgg aatatttttt
      841 gagcgtggca tgaaagccag ggttgggcag cccagtgtgc atgaggaatc cgccggggaa
      901 ttcgaccaaa gccggatggg tagtcggcag gcgcgggcgg agagagagcg gattcgtcac
      961 aaatgccccg aatgaatcca atgagattcc attgcgtggg tcgggtgtga atcccaatga
     1021 tcccgccgca ttgagaagag gtttgctaaa atacagatcg cgtttcatag gagatgatat
     1081 gcagccctta cagaatatcc gcgtgctcga cctaagccgt gtgttggctg gaccttattg
     1141 tacgatggtt cttggtgact tgggagcgga ggtcattaag gtggagccac cagaagggga
     1201 tgaaacgcgt ggctggggtc cgccatttgc gggtggagag agcgcttatt atttatgtgt
     1261 aaaccgcaat aagcgtggca tggtggtgaa tttgaaaacg gaagaagggc gggatgttct
     1321 gcaaaaactt gccatgcaaa gcgatgtagt agtggaaaat tttcgccccg gtacattgaa
     1381 gaaatttggc ttggattttg aaactttgca tgaattgaac ccgaagctga tttattgctc
     1441 aatctccggc ttcgggcaga cgggtccgtt gaaagatatg ccggggtatg attttatgat
     1501 ccaagccatg ggcggaatca tgagcgtcac tggcgaacca gagggtgagc ccatgaaagt
     1561 gggcgtggct gtggcagatt tattcgccgg gcaaaatgcg atcatctcga tcttggcagc
     1621 attacaagcg cggacgttta ccggcgaagg tcaatatatt gacattgcct tgtttgattc
     1681 tgaattgggt tggcttgcaa atgttgcaag caattttctg atttcgggga agaaccccaa
     1741 gcgttatggc aatgcacatg ccaatattgt gccgtatcaa agttttcaag ccagcgatgg
     1801 ttggtttgtg gtggcagttg ggaatgacaa gcagttcgag tctttttgca acgtgattga
     1861 aaaccctgag ctttcgagcg atgaaaggtt ttctacgaat tcggcgcgtg tgcaaaatcg
     1921 cgaagtatta attcctttgc tcaaacctat cttcatgcag aaaaccgtga acgaatggct
     1981 tgctctcatt ggtgaccaat tcccatgtgg acccatcaat acttttgacc aggtgttttc
     2041 gatgccgcat gtgaaagaac gcgagatgtt gatagagatg gagcatccca ccattggggc
     2101 gttgccactg gtcggttcgc cgcttaagat gggtggtacg ccggtctcat accgcctccc
     2161 gccgccgctg atgggtcagc acacccatga aattttgaaa gaagtgcttg agttttccga
     2221 tgaagcggtg cagaagttga tgaatgacgg gtgtatcaaa taactttatt ggagttttac
     2281 atggaagtca tttattctgt atccccgttg aaggcatttt ctggaagctt tctcttttta
     2341 gcatttttct ttgtgttggg cgggtttggt gcgctctctg ccatctttag cagacgtgac
     2401 cgagcgggga agcgtattgc cttgggctgt gcttcagcaa tcattctgtt ggctgggacg
     2461 ggcggagcag tagctacgtt tatgacttat accggtgggc aaacaaccgc tcttgtgcac
     2521 gtggataaaa agcggatcgt agaacgtaat tgtgataacg catcaaatac ctgcattgat
     2581 tatgtcattg aagccagtga cgggcagaag tattacactt ttggtttggc gaaagatact
     2641 tgggatgtag tccaagatca ggcttgttat gaattcactt attacccggc aaaatcattg
     2701 ttcggtgagt atttgcaaca agataatccc tatgccgata tgtacgagac aacctcaaat
     2761 atcgtggaaa taaaaacggc tcagtgcccc tgatgagaac ctatttcttg atgtgataca
     2821 tcgtcaacag cggagtccac tgtttggttt caatggtcag cccgcgttga gtcagatgcg
     2881 cgtctagggc gttgagtccg aaatcggtgt tggggtgtcc ccagcggaag aaagcgtcga
     2941 agactttgat atccacaccg cgcggttctt cgagaaaaag gctgccgccg ggtttgagta
     3001 cgcggataat ttcgtcaatc gctttgcgcc aatcagggat gtgatgcagc acaccgaaga
     3061 taatgacgac atctttgctt gcatctgcaa agacactgag gtcttctgcg ccttgaagaa
     3121 tgaattgaaa ttggggatat tgttttttcg caagcgccac ttgttcttcc atcacatcca
     3181 acccgatata gcttttgggg ttgagagtag aaagcagata ggcaccatag ccagagccac
     3241 agccaatttc aagcacatct ttattttgca ggttgagtcc catgcgtttg aagaggggca
     3301 tttcaaaata acgttgaccc agcttgcggg gcagggactg catagcgttg aattcagggg
     3361 cagatagctt cataaaaaaa tctccgagtt catgaagaac tcggagattg atttcttacg
     3421 cggtttcgat cagacccatg gcttgcagca tttcttcgtt gctgccgtac ttgaaccatt
     3481 caagttcgcg cttcttggct tcttcggctt cagccgcttc gagccgtttg atatcttctt
     3541 tgtacacgat cggcttttgt agcgctttga ccttggctgc caccgcttcc gggctggcat
     3601 tgctgggctg tttggtctgt agatattgca tcacggcttt ggcggcattg gtgccatcgc
     3661 ggcgagcata cccaaccaaa ccttcgcttg ccttacgaga ccatccgccc acgaagatgc
     3721 cttcggtcgt ggattcgtag gaagtgcctt cgatggggaa cttcggctct ttagttttgg
     3781 agaactcgcc ccattcggtc ggcagaccaa aatttgcatc tacttggtca ccgatggcaa
     3841 agatgacagt ttcaacgtca agtgtatgct ttttgccagt gcccttggcg ctggtttttc
     3901 cgtctcgttc agtgaggatg ttttcttcta cttcaagctg ggtaagcaaa ccattctcgc
     3961 ccaacatgcc agaaggggag gcgaggaagt cgaacttgaa cttcgcttct ttcaccttcg
     4021 ggtctgcttt ggcaagcgca tccaaaacct tttgacgccc aatctgcggg tcctgaccga
     4081 tcgcatcgag agccgggcgc acacgagcca attccttctc gaactcgtcc atgtcgagat
     4141 aagcaacgag gtgcttcatt tcttccttcg tgaaattcac ctcagccgga ccgcgacgaa
     4201 ccacggcggt cacttcttct acaccttggt ggttgatgag ataacgagca atgtccacca
     4261 tcacgttacc agcgccgata atggcggcac gctttccata gcggaacttg cgctggctga
     4321 acggtggcaa cttgttatag ccataaacca cgtctttggc gtggtacacg ccttcaagct
     4381 cttcgccggg caagcccaaa gacttggtgc cctgcgcacc cgcagcaacc aacaccgcgt
     4441 caaaaccgag cgcgcgaatt tcatccaatg tgaaatcacc ctttgtgccg atggctacat
     4501 tgccataata gtcaatgttc ggcatgtcga gcgcctgacg gaactgcttg cgcagccctt
     4561 ctttcatggt gtgtttttca aggtaaatgc cgtactcagc cagaccacca gctttgatat
     4621 cacgattgaa aagagcaacg cgcactccgc tatttgctaa ttcacgtgcc ccgaaaagtc
     4681 ctgcgggtcc cgcgccaatc acggcgacaa aaaattgact ctccacgttc ttcctccgaa
     4741 ataaaaaaaa ttacatttta gacaaaaact atcaggtttt accacaaaca tcacagaatt
     4801 tacagacaaa aaaccgttca attctgttat cacagtcgaa tgctacctgc aaaaagttac
     4861 ttttcagatg ttaattccgc attagagatg tttgtgcttg ctgtaagaaa cccatcccat
     4921 cttcataaac atctatgcta atattaatcc acgtactaaa aaagaggcag tatgacagac
     4981 g
//
LOCUS       DAOQFT010000118         4970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8828_length_4970_cov_6.882604, whole genome shotgun sequence.
ACCESSION   DAOQFT010000118 DAOQFT010000000
VERSION     DAOQFT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8828_length_4970_cov_6.882604"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLR93_04345"
     CDS             <1..412
                     /locus_tag="PLR93_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26223.1"
                     /translation="NVAANLANNAVDYAVRSNVPSRLVYQVKTHAEGNVLGWIVPSRG
                     TGWFDFLPYSTRIQILNLPFAARAYAPSYEFKIGNLYGPTVLKLQKQRDLLVDKYTLE
                     KAGELTDQQERWAIPALTLVAMFERTRVKQMADW"
     gene            complement(471..1952)
                     /locus_tag="PLR93_04350"
     CDS             complement(471..1952)
                     /locus_tag="PLR93_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26224.1"
                     /translation="MSPRNSNLFLFALIFSSAVLALLRFDSLQIGTSYDDAKYIILAE
                     SLASGQGYELINFPRPQIERNFPPGWSLVLDPFTLVFPKNYDALKFVSLAFWLASILL
                     VYKLFSKRLNSPYLEVLTALVALNPLLVGTSVTLMSETAYLFFSLLALLAFDQVGEKK
                     WGWLVLAAVLAFYTQQVRTIGIALTVSLLVILVLSKRYKEMGLVAALFIVGFTLQSWL
                     NLRNGGTVISSGYEAQVLNGSMIEKVGQVWSNASGYLNQVLAGSLIPIFGTRLDTTFG
                     WLFILLNIAILFVLVLGMFTTKFKFEWMHIYFVIYTAGILAFWNPQVGSVKARFLIPV
                     LPFLYFYFLSGLKWLARENSRLVIGVACGIALVLLARNLQDWRSPVREQMTDLSIGTS
                     WVSENAPADAIVMVNEPVPAYVHVKHKTINFPKNDQDLEQYIKKQDIEYIIIAPLLQS
                     PKSTELSKDIQAIQSQIESQPNAFQVVFESKENNVTVYQYTGQ"
     gene            complement(1955..2494)
                     /locus_tag="PLR93_04355"
     CDS             complement(1955..2494)
                     /locus_tag="PLR93_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH26225.1"
                     /translation="MDHHDHVNLLRPASLDKGGSWADLGAGSGAFTFALRELIGPMAT
                     IYAIDKDRNSLNSLKSEYRVRFASDENLHLQVEDFSRTSNLPPLDGILAANSLHYFKN
                     RVKVLQHIKSFLKPNGKLIVIEYNVDSGNLWVPYPFSFGTFGKLAAQAGFAEPQLLAK
                     NPSSFLREFYSALTFKAEA"
     gene            2633..3874
                     /locus_tag="PLR93_04360"
     CDS             2633..3874
                     /locus_tag="PLR93_04360"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26226.1"
                     /translation="MENSTYHDPYAPLRFRDYRLLLSGRFISAFANEMLSFAIGWELW
                     LRTHSAFALGMVGLVQVVPVILLSLPAGHVADQYNRRRIVIISEVLIALCVIALGWLS
                     YTEGSLVLIYIALLGIGIARAFNEPASSTLTPETVPPELFSQAATWGSGIWHFASITG
                     PALAGLLVGFFHSVTFIYVYAALSAITYCVLISLIKGRQLPLAEKSATLDSVREGLRF
                     MWNTKVILAAITLDMFAVLFGGAVALLPIYATDILHVGAQGLGIMRAAPSLGALAMAF
                     LIAHLPPMKNAGKTLLFAVTGFGLATIVFGLSTNFFLSVTMLMLLGGFDNISVVIRST
                     LMLTQVPDEMRGRTSAVNNIFIGVSNELGAFESGFVAALVGPVITVVGGGIGTILVVL
                     AIAGIFPAMLKLKSLDVPKPS"
     gene            3934..>4970
                     /locus_tag="PLR93_04365"
     CDS             3934..>4970
                     /locus_tag="PLR93_04365"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH26227.1"
                     /translation="MMNNKQLAAVFTLIANLLEIKGEIIYKTLAYRKASESLDTLGRE
                     ASEYWKEGKLADIPGVGKAIAEKIDELLNTGKLEFLEKLKKEVPESLAGWLQVPSLGP
                     KKIAMIWKALNITTLAELEAAAKAGKLKDLPGMGAKSETAILEGIASLARRSGRLPLG
                     KAYPLAQEIIKVLKGVKGVVDVQPAGSLRRMRSTVGDLDILVAAKDSAAVMEAFVNLK
                     GVERVLGKGDTKASIEFFDGVRAQVWVHPPEKFGTALQYATGSKDHNVKLRQIALDKG
                     LSLSEHALTKVKGKGEILCATEEEVYKTLGLQYVPPEMREDRGEVEAAKANKLPKLIQ
                     VKDIKADLHMHS"
BASE COUNT         1272 a         1116 c         1280 g         1302 t
ORIGIN      
        1 taatgttgct gccaacttag ccaacaatgc agtcgattat gcagttcgct cgaatgtccc
       61 gtctcggctt gtgtatcagg ttaaaaccca tgctgaaggc aatgtgctgg ggtggatcgt
      121 cccttcgcgt ggtactggct ggtttgactt tttaccctat tccacccgta ttcaaattct
      181 taatctgcct ttcgctgctc gtgcttatgc tccttcttat gaattcaaga ttggtaattt
      241 gtacggacct acggtgttga aactacagaa acaacgtgac ttgctcgttg ataaatatac
      301 ccttgaaaaa gctggtgaac tgacagacca acaagaacgc tgggcgatcc ctgccttgac
      361 cttggtggcg atgttcgagc gcactcgcgt caagcagatg gcagattggt aattggcaaa
      421 tcagtaggtt ggtattgaaa aaagcttccg gcgttatcgc cggaagcttt ttattgccca
      481 gtgtattgat ataccgttac gttgttttct ttactttcaa aaactacttg aaaggcattc
      541 ggctggcttt cgatttggga ttgtatcgct tgaatatctt tacttaattc tgtgcttttt
      601 ggcgattgca atagcggagc aataatgatg tactcaatgt cttgtttttt tatatattgc
      661 tccaagtctt gatcgttctt tggaaagtta atagtcttgt gctttacatg cacataggct
      721 ggcacaggct cattcaccat cacgattgca tccgctggcg cattttcaga aacccaactg
      781 gttccaatag aaaggtctgt catttgctcg cggacggggg atcgccagtc ttgcaaattg
      841 cgggcgagca aaacaagtgc aatgccacaa gcaactccaa tgacaaggcg cgagttttct
      901 ctggcgagcc atttaagtcc acttaagaag taaaaatata aaaatggcaa aacgggaatc
      961 aagaagcgtg ctttcacact tcccacttgc gggttccaaa acgccaaaat gccagccgtg
     1021 taaatgacaa aataaatatg catccactcg aacttgaatt tggtggtgaa catgcccaac
     1081 acaagcacga acaagattgc gatatttaat agaatgaaca gccaaccgaa tgtcgtatcc
     1141 agccgcgtgc cgaagatcgg aattaatgac ccggcaagaa cttgattcaa atagccggat
     1201 gcatttgacc agacttgccc aactttctcg atcattgacc cgttcaacac ctgcgcctca
     1261 tatcctgatg agataacagt cccgccattg cggaggttga gccaactctg gagagtgaat
     1321 ccaactataa agagcgcggc aactagtccc atttctttgt agcgcttgga cagcactaag
     1381 atgacaagca acgagacggt gagggctatc ccaattgtgc ggacttgctg tgtgtaaaat
     1441 gcgagaactg cagcaaggac aagccaaccc catttttttt caccgacttg gtcaaaggcg
     1501 agcaatgcca gtagagagaa aaataaatag gctgtctcag acatcaatgt gactgatgtt
     1561 cctaccaaaa gcggattcaa cgcgaccagt gctgtgagaa cttcaaggta tggagaattc
     1621 agccgttttg agaataactt atagaccaaa agaatggacg ccagccaaaa cgcaagcgaa
     1681 acaaacttga gcgcatcata attttttggg aaaaccaagg tgaaggggtc cagcactaat
     1741 gaccagcccg gcgggaaatt ccgctcgatc tgtgggcgtg gaaagttgat gagttcatag
     1801 ccttgtccac tggcgaggct ttcggcgagg atgatgtact tggcatcgtc gtaagaggtg
     1861 ccgatctgaa gcgagtcgaa acgtaaaagt gcgagtacgg cgctgctgaa aatcaatgcg
     1921 aacaaaaata gatttgaatt tcttggtgac atgctcatgc ctctgccttg aacgtcagcg
     1981 cggaatagaa ctcgcggaga aaacttgaag ggtttttcgc aagcaactgt ggttcggcga
     2041 agcctgcctg tgctgctaat tttccaaacg ttccaaatga gaatgggtag gggacccaaa
     2101 gattgcccga gtccacgttg tattctatga cgataagttt gccattcggc ttgagaaaag
     2161 acttgatgtg ctgtaaaacc ttaaccctat ttttaaaata atggagagag ttagcagcaa
     2221 ggatgccgtc aagagggggg agattggatg ttcgcgaaaa gtcttctact tgcaggtgca
     2281 gattttcatc gcttgcaaag cgtacacgat attcgctttt taatgagttc aggctgttac
     2341 gatctttgtc tatggcgtag atcgtagcca taggaccgat cagttcacgc aaggcaaagg
     2401 taaaagcgcc agaacccgct ccgaggtccg cccatgagcc gcctttatcc aaagatgctg
     2461 ggcgcaataa gttgacgtgg tcatgatgat ccataggtga atcgtatcat acccatgatc
     2521 ctgagggtcg tcatgaaatt taagacttcc cttttcacat cttatgaaat ggactaagtc
     2581 tttaagtcta cggatggtat catacacacc atctccgaaa ataggatttg aaatggaaaa
     2641 ctctacatac cacgatccct atgcgccgct gcgttttaga gattatcggt tgctgctcag
     2701 tgggcgtttt atcagcgcat ttgccaatga gatgctgtcc tttgccattg ggtgggaact
     2761 ctggctgcgt acccatagtg cctttgcctt gggcatggtg gggttggtgc aggtggtgcc
     2821 ggtgattctg ttatctctgc cagccgggca tgtggcagat caatacaatc gtcgtcgcat
     2881 tgtcatcatt agtgaagttt tgattgcctt atgtgtgatc gctttgggct ggctttccta
     2941 tactgaaggg tcgttggtgt tgatctacat agccttattg gggatcggga ttgccagggc
     3001 atttaacgaa ccggcttctt caaccctgac gccagagacg gtgccaccag agttgttttc
     3061 acaagctgcc acatggggca gtggaatctg gcacttcgcc tcgatcaccg gaccggcact
     3121 cgctggctta cttgtggggt ttttccactc tgtgaccttt atctatgttt acgcggcgct
     3181 atcggctata acgtactgcg tgctcatttc gttgatcaaa ggcaggcaat tgccgttggc
     3241 agaaaaatct gccacgctgg attctgtccg tgaaggtttg cgctttatgt ggaacacgaa
     3301 agtgatcctg gctgcgatca cactggatat gttcgccgtt ctctttggtg gagccgtagc
     3361 gttactgccg atctatgcta cggatattct gcatgttggc gcacaaggat tgggtatcat
     3421 gcgcgctgca ccatcgttgg gtgccctggc gatggcattt ctcattgccc atttgccgcc
     3481 tatgaagaat gcaggcaaga ccttactatt cgctgtgact gggtttggct tggcgaccat
     3541 tgtctttggt ctttcgacca acttttttct ctcggttacc atgcttatgc tgcttggcgg
     3601 ctttgataac attagtgttg tgatacgcag caccttgatg ttgacccagg tgcccgatga
     3661 aatgcgcggg cgcacctccg cagtgaataa tatcttcatc ggcgtatcga atgaactggg
     3721 cgcttttgag tcgggctttg tcgcggcatt agtaggacct gtcattacag tagtaggcgg
     3781 aggtattggg accattctgg tggtgctggc aattgccggc atcttcccgg cgatgctaaa
     3841 actaaaatcg ttggatgtgc caaagccatc ataagcctgt acaaaggtat tgatatccat
     3901 gttgacttat aattgaccta ttagaggtgt ctcatgatga ataacaaaca attagccgct
     3961 gtctttactc tgatcgccaa tttactggag atcaaaggcg agatcattta taagacgctt
     4021 gcctatcgca aagcatcaga gagtctggat acgctcgggc gtgaagccag tgagtattgg
     4081 aaagagggaa aattagccga catccccggt gtgggcaaag ccatcgccga aaaaattgac
     4141 gagttgctca acacgggcaa acttgagttt cttgaaaaac tgaaaaaaga agtgccagag
     4201 agtttggcgg gttggttgca agtcccttcg ctgggaccca agaagatcgc catgatctgg
     4261 aaagcgttga acatcaccac gcttgctgaa ctcgaagcgg cggcgaaagc gggcaaacta
     4321 aaagacttgc ccggcatggg agccaaatca gaaaccgcga ttctcgaagg cattgcatcg
     4381 ttggcgcgcc gttcgggtcg tttgccgttg ggcaaggcat atccgctggc gcaggagatc
     4441 atcaaggttc tcaaaggcgt aaaaggcgtt gtcgatgtgc aacccgctgg cagtttaaga
     4501 aggatgcgtt cgacggttgg cgatctggat atcctcgttg cagctaaaga ttcagcggct
     4561 gtgatggaag ccttcgtcaa cctcaaaggt gtggaacgtg tgttgggcaa aggtgatacc
     4621 aaagccagca ttgaattttt tgacggcgtg cgtgctcagg tctgggttca cccgccagag
     4681 aagtttggta cggcgctgca atatgccacg ggctcaaaag accataacgt caaactgcgt
     4741 cagatcgcgc tggataaggg actgtcgctt tcagaacatg cgctcactaa agttaaaggt
     4801 aagggagaaa ttctttgcgc taccgaagaa gaagtgtata agacgctcgg cttgcaatac
     4861 gtcccgccgg agatgcgtga agatcgcggc gaagtggaag cggcgaaggc gaataaactt
     4921 cccaagttga ttcaggtcaa agacatcaaa gccgatctgc atatgcattc
//
LOCUS       DAOQFT010000119         4952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8871_length_4952_cov_6.712477, whole genome shotgun sequence.
ACCESSION   DAOQFT010000119 DAOQFT010000000
VERSION     DAOQFT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8871_length_4952_cov_6.712477"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLR93_04370"
     CDS             <1..490
                     /locus_tag="PLR93_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNH26228.1"
                     /translation="DGETWQILTTPSGRGTDPSGNSYGWGYTGVSGDWIEEKVDLSDY
                     AGKKVFVRFEYITDAAVNGEGFMVDDVKVDAAGYSSDFEADDGGWVAEGFVRVQNVLP
                     QTFKLALIKSGDDNVTMIPVNADQTAEIPVSLKSGETAYLVVTGTTRFTRELATYQIE
                     IR"
     gene            616..3741
                     /locus_tag="PLR93_04375"
     CDS             616..3741
                     /locus_tag="PLR93_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH26229.1"
                     /translation="MMTQSGNPSGKRKTIAVLGAQLSRVWGSEFMAGVLDAAHAVDFN
                     VVSFVGGKPSALQSHDGHSYGLYDLINPEQIDGVLLSADIAHGLSRDALQKFVEFLAP
                     LPVASFAVPSKGVPAVIADNDSGMRAIVRHLIEDHNYQRIAFVRGPVGQVEADQRYHA
                     FLDELKAHNIRFDQHLELEGDYSTESGRAAVRTLLDERGMAVQAIVASNDRMAFGVLE
                     ALQQRGITVPDKVAVTGFDDVSESQSMGVPLTTVRQSFYDAGAKTFELLLKRINGEEV
                     DDINLLPSSIVVRWSCGCLPESIHRAVVLPREVAHTGRLENKRVKAINALLAAAGVPE
                     TTPDRDVYVENFGVAWDTFLYSLRETDKSDAFLRSIQGMVEILQKHGYDFYTWQNVIS
                     TFRKYALGGISSTTTTLRAENLFQQARMLAGELSQRAQAYNRLQYVKQEELLNNFSFS
                     MASAVSFDDIGDAIARHFPILGIERWYVMYYGETDDAPGSISSPPPQSYRLLMQYDDK
                     RFTMPDDRSRLATGRLVPRGKTPEKRRYDAVVMPLSLATNRFGFMWAEMGPDDWDIYV
                     RVRNLLSSALLRTMLVNQREQAKREVDQLYAAEQARRHGAETLARSMRQLSSLKNVEQ
                     LPEQILEQLSQALPYDRGVLFMEDVNGEPQIQAHRGMPTNADMASFRLGMNGNDFYTT
                     VSRKGETLVVSDINLVENWSQPEWLARDRSWMGVPLYLKDNVIGLLMVSRADRSFTHD
                     DGLLATTFASQATVALENARLYHEVTGINQMMERMVSERVAELNETYKTLEKHDKNKS
                     AFIQVAAHELRTPLTVVKGFLGMLQNDATVRSNSMLSDAVEGVLRGTNRLHQIVNSML
                     DVVRLENQVITPHLEAVSLGLILRLIHKDYVDDLATRNLTFTLDDSVKTIPPMLADSE
                     LVKKALDQVIVNAIKFTPDGGSIYISAWVVEDPKRGKMAEIAIKDSGIGIDSEHHKVI
                     FEKLYQMGEVQLHSSSRTNFKGGGAGLGLAIASGIVRALQGTIWVESPGKDEEKYPGS
                     TFFIRLPLLKE"
     gene            complement(3778..4680)
                     /locus_tag="PLR93_04380"
     CDS             complement(3778..4680)
                     /locus_tag="PLR93_04380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH26230.1"
                     /translation="MTNTPPVCNYEGSDYQQSFWDQGGRAYEDQSEAIALRRMLPKSG
                     KLMLEVGAGAGRNTPRYAGFDRIVVMDYSTTQLAQAQERLGRSDKYIYVAADVYRLPF
                     RNGLFDGATMIRVLHHMADAPKALGQIQGTLQPGATFILEFANKLNLKAILRYWLGKQ
                     TWSPFTLEPVEFVKLNFDFHPKAIRQWLGNLGFNIEKTLTVSHFRLGLLKRTIPSKLL
                     AAVDGLFQPTGALFQLTPSVFVKAVLPGASSADIPLNVIDLFKCPECNSGGLQEKTDH
                     VFCPSCKAKWSVKDGIYDFREKIS"
     gene            complement(4770..>4952)
                     /locus_tag="PLR93_04385"
     CDS             complement(4770..>4952)
                     /locus_tag="PLR93_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNH26231.1"
                     /translation="YKLAEGCDALMIITEWNEFKQLDLDKVKGLLKSPVIYDGRNIYE
                     PGRMKEMGFTYRAIGR"
BASE COUNT         1216 a         1129 c         1293 g         1314 t
ORIGIN      
        1 agacggcgag acttggcaaa ttctcactac accttctggt agaggcacag acccgagcgg
       61 caattcgtat ggctgggggt acactggtgt ttctggcgat tggatcgaag agaaggttga
      121 cctttctgat tacgctggta agaaagtctt tgtccgcttt gagtacatta ccgatgctgc
      181 ggtcaatgga gaaggcttca tggtagatga tgtcaaagtc gatgcagcag gctactcgtc
      241 tgattttgag gcggatgatg gcggttgggt ggcggaggga ttcgtgcgcg tccagaatgt
      301 cctcccgcaa actttcaagc tggcgctcat caaatctggc gacgacaatg tgactatgat
      361 cccggtcaac gcagaccaga cggcagagat cccggtttcg ttgaaatctg gtgaaacggc
      421 ttacttggtt gtgaccggaa cgacccgctt cacacgtgag ctggcgacgt atcagatcga
      481 gatccgctaa gcttttctga aatttctaca ttgacagccg ccataaaagc ggctgtcaat
      541 ttttttaagg cggagattgt cttgcaaaat tgcaattttt gtctcgggca tcctgccgta
      601 taattttgca attccatgat gacccaaagc ggaaatcctt ctggcaagcg aaagacgatc
      661 gccgtcctcg gtgcgcaact cagccgagtg tggggcagtg agtttatggc gggcgtgttg
      721 gacgccgctc atgcagtgga tttcaatgtc gtttcttttg taggcgggaa accttcggcg
      781 cttcaatctc atgatggaca ttcttacggt ttatacgacc tcattaatcc cgaacagata
      841 gacggtgttt tgctctctgc agatattgcc cacgggcttt cacgagatgc acttcaaaag
      901 tttgttgaat ttctcgcgcc tttgccggtt gcttcattcg ctgtgccaag caagggcgtt
      961 cctgctgtga tcgcggataa tgacagcggt atgcgtgcga tcgtacggca cttgatcgaa
     1021 gaccataatt atcaacgcat tgcctttgtc cgcggaccgg ttggtcaggt ggaagcagac
     1081 cagcgctatc acgcattcct tgatgagttg aaagctcata acattcgttt cgaccaacat
     1141 ttggaattag aaggcgatta ttccaccgaa agtggccgtg ccgcagtgcg caccttgctt
     1201 gatgaacgtg gcatggcagt gcaagccatt gtcgcttcga atgaccgaat ggcattcggc
     1261 gtgttggaag cgttacaaca gcgcggtatt acagtgcctg ataaggttgc ggttacgggt
     1321 ttcgatgatg tcagtgagtc acaatctatg ggggtgcctt taaccaccgt ccgccaatcg
     1381 ttttatgatg cgggtgcaaa gacatttgaa cttctcctga aacgcatcaa tggcgaagaa
     1441 gtggatgata ttaatttgtt gccatccagc attgtagtgc gttggtcttg tggttgttta
     1501 ccagagagta ttcatcgtgc agttgtgttg ccacgtgaag tagcgcacac aggccggctt
     1561 gaaaacaaac gtgtgaaagc catcaatgct ttgcttgcag cagcaggcgt gccggaaact
     1621 acacctgatc gcgatgtcta tgttgaaaat tttggtgtgg catgggacac cttcctttac
     1681 agtttgcgcg aaaccgataa gagtgatgcg ttcttacggt caattcaagg catggttgaa
     1741 atccttcaga agcatggtta tgacttctat acctggcaaa acgtgatctc aactttccgt
     1801 aaatatgcgt taggtggcat ttcaagcaca accactacat tgcgtgctga aaatcttttc
     1861 cagcaggcac gtatgttggc gggcgagttg tcgcagcggg ctcaggcata taaccggctt
     1921 caatatgtaa agcaggaaga actgcttaat aactttagtt tttccatggc atcggctgtt
     1981 tcgttcgatg atatcggcga tgcgattgct agacatttcc ccattctcgg tattgagcgc
     2041 tggtatgtga tgtattatgg cgagacggac gatgcgccgg gttcgatttc ttcaccgccg
     2101 ccgcaaagtt atcgtttgct catgcaatat gacgataaac gcttcaccat gccggatgat
     2161 cggtctcgac ttgcaacagg tcgccttgtg ccacgcggaa agactcctga aaaacgtcgt
     2221 tacgatgccg tagtcatgcc gctctctttg gcaacaaatc gcttcgggtt tatgtgggca
     2281 gaaatgggac ccgatgattg ggatatttac gtccgcgtgc gaaacttgct ttcaagcgcg
     2341 ctcttgcgta cgatgttggt taatcaacgt gagcaagcca aacgtgaagt ggatcaactt
     2401 tatgcggcgg aacaggcacg gcgtcatggt gccgagacgt tggcacgttc gatgcgtcag
     2461 ttgtcttcac taaaaaatgt tgaacagtta ccagaacaga ttctagaaca attaagccaa
     2521 gcattgccgt atgatcgtgg cgtgctgttt atggaagatg taaatggtga gccgcagatt
     2581 caagcccatc gcggtatgcc aaccaatgcg gatatggcat ctttccgctt gggaatgaat
     2641 ggtaatgatt tttacacgac cgtctcacgc aaaggcgaga ctcttgtggt gagcgatatc
     2701 aacttggttg aaaattggtc tcagcctgaa tggcttgccc gtgatcgttc ttggatgggt
     2761 gtgcctttat atctaaaaga taatgtcatt gggctcttga tggtcagccg agcggaccgg
     2821 tcatttactc acgatgacgg tttgcttgcg acgacatttg cttcacaagc aaccgttgct
     2881 ttggaaaacg cccgtttgta tcacgaagtg actggcatta atcaaatgat ggaacgcatg
     2941 gtttccgagc gtgttgcaga actgaatgaa acctataaaa cacttgagaa gcacgataag
     3001 aacaaatctg cgttcattca ggtggcagca catgaactgc gcacacctct gaccgtggtg
     3061 aagggttttc ttgggatgtt gcaaaacgat gcaaccgtcc gttcgaattc aatgctttcc
     3121 gatgccgtag aaggggtctt gcgcggcacg aaccgtttgc atcagatcgt aaacagcatg
     3181 ttggatgtgg tgcgccttga gaatcaggtc atcacgccac atttagaagc ggtcagtttg
     3241 gggctgatct tacgcctcat tcacaaagat tatgtggatg atctcgcgac tcgtaacttg
     3301 acctttacat tggacgactc tgtaaaaacg atccctccaa tgttagcgga ctctgaactt
     3361 gtgaagaagg cactggatca agttatcgtc aatgctatca aatttacacc cgatggcggc
     3421 tcgatttata tctcagcttg ggtagtggaa gatcccaaac gcggcaagat ggcagagatc
     3481 gccatcaaag actctggtat tggtattgac tccgaacacc ataaagttat ttttgaaaag
     3541 ttgtatcaaa tgggcgaggt gcaattacat tcgtctagcc gaaccaattt caagggcgga
     3601 ggtgctgggt tgggacttgc cattgcctct ggtattgtcc gtgccttgca gggaacgatt
     3661 tgggtggaaa gccccggcaa agatgaagag aaataccccg gcagcacctt cttcatccgc
     3721 ttgccgttgt tgaaggaata acaaaaaacg cgctcaatgt agagcgcgtt tttttttcta
     3781 acttatcttt tctctaaaat cataaatccc atccttgaca gaccacttag ccttgcaaga
     3841 aggacaaaat acatggtctg ttttctcttg caaccctcca ctgttacatt cggggcattt
     3901 gaagaggtca atgacattta acggaatatc tgcactggag gcaccgggca aaactgcttt
     3961 tacgaatacg cttggcgtga gttggaagag cgcacctgtt ggctgaaaga gaccatccac
     4021 agcggcgagc agtttagaag gaatagttcg tttgagcaaa ccaagccgaa aatgtgagac
     4081 ggttaaggtc ttttcgatat tgaaaccaag atttcccaac cactgacgaa tagccttggg
     4141 gtgaaagtca aagttcaatt tgacgaattc caccggctcc agcgtgaacg gactccacgt
     4201 ttgcttgccg agccaatagc gcagaatcgc cttgaggttg agcttgttcg caaactcaag
     4261 gataaaagtt gccccgggtt gtagtgttcc ctgaatctgc cccaacgctt tgggcgcatc
     4321 tgccatgtga tggagcacgc ggatcatggt tgcgccatcg aatagtccat tacggaaggg
     4381 gaggcggtag acatctgcag cgacgtaaat gtatttgtcg gagcgaccca gcctctcttg
     4441 cgcctgagcg agctgagtcg tggagtaatc catcaccaca atccggtcaa agcccgcata
     4501 gcgaggggtg ttgcgtccgg caccggcgcc tacctccagc atcagcttgc cgcttttggg
     4561 caacatgcgt cttaatgcaa tggcttcaga ttggtcttcg taagcgcgcc cgccttgatc
     4621 ccaaaaggat tgctgatagt cggagccttc atagttacaa acaggaggag tattggtcat
     4681 aattctgttc tttttgtgtt taaaaaattg gtagtcgagc cgtcagattg tacccgacta
     4741 ctcgactacc aaacaaatga actgaaaaat tagcgtccga tggctcggta ggtgaagccc
     4801 atttctttca tacgaccggg ctcgtagatg ttacgaccat cgtaaatgac cggggatttt
     4861 agtaagccct tcactttgtc gaggtcaagt tgtttgaact cattccattc ggtgatgatc
     4921 atcaacgcat cgcagccttc ggcaagtttg ta
//
LOCUS       DAOQFT010000120         4950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8875_length_4950_cov_4.658631, whole genome shotgun sequence.
ACCESSION   DAOQFT010000120 DAOQFT010000000
VERSION     DAOQFT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8875_length_4950_cov_4.658631"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="PLR93_04390"
     CDS             <1..1259
                     /locus_tag="PLR93_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26232.1"
                     /translation="TAGAEIKYDAATYPYFMGADGKAYPNWTPRLLKAAYNYQVSMKD
                     PGAFAHGNKYIVQLLFDSITDLGGDTSKLARTDAGHFAGNTMPFRDWDLTEDGQPNYM
                     VPFGCVKCHTAQGLPTFIKDGGTTVVTSSGTTSTTGVQSMPSSNGFMCSTCHNEEAWP
                     ERYAVASVVFPSGKTVSFGGKDADGNWVADDANLCISCHMGRESTSSVNNALKGKDPD
                     TVDAKIRFKNIHYFAAGATIFGNDAQGAYQYEGKEYLGQNMHADEEGKMNKCQDCHDV
                     HALEPKVESCETCHDTTDPTTIRESEVDYDGDGDVAEGIKGEVDTLAEALYAQLQTYA
                     AANGGAIEYNPAAYPYFFGADGKSYATWTPRLLRAAFNYQYSQKDPGVYVHNPKYIIQ
                     ILIDSIEDLGGTVSTYTRPEVPAPAQ"
     gene            1460..2620
                     /locus_tag="PLR93_04395"
     CDS             1460..2620
                     /locus_tag="PLR93_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNH26233.1"
                     /translation="MNFRKSLRPHHLWTVSLLFGIGFFLFALIALTTPAQAAPSHQDD
                     KPSNDFCLACHQEEGIKKTLGSESLPVTINPTRFGLSVHGEEGIACVDCHQDISDYPH
                     PDVKVKNTRDFTLEFLDTCKECHEDQYNKAHDSVHQRAFDEGNKDAAVCMDCHNPHTQ
                     TRLTGKTSGEITPSARVEIPETCAQCHSTIYDQYKTSIHGNALTEEGNTDVPTCIDCH
                     GVHNIQDPTTVTFRNSTPFLCANCHTDAKIMDQYGISTNVLNSYVADFHGTTVKLFEE
                     EFPGQPTNKPVCTDCHGVHNISKVDDPDTGIALKDNLLEKCQRCHPDATSNFPDAWMS
                     HYEPSPENFPLVYYVNLFYKFFIPAVLGGMILFIITDIYRRIINRVKGVKHS"
     gene            2617..3477
                     /locus_tag="PLR93_04400"
     CDS             2617..3477
                     /locus_tag="PLR93_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNH26234.1"
                     /translation="MSQSSANLYMRFPVARRIEHWVMMLSFTTLGLTGLAQKFSDHPV
                     AISILKALGGIESLRTIHHIAAIVMMFGTAWHILVMGYSVFVLRDQMSMLPSIQDAKD
                     GIQALLYNIGIAKTYPQMGRYTFEEKMEYWAFVWGAAVMGITGFLMWNPITATQYLPG
                     QFVPAAKAAHGGEAVLAVLAIIIWHMYGVHIKRFNKAMFTGTQTEAEMLHEHPLELAD
                     MKAGIADRRPDDATLRKRKMIYYPIAAIVTLGMLAGIFGFINSEETALTTITPLTESE
                     IYSPQQTPQP"
     gene            3537..>4950
                     /locus_tag="PLR93_04405"
     CDS             3537..>4950
                     /locus_tag="PLR93_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNH26235.1"
                     /translation="MKLRQRIAKFFHPDPGSPFWVLVLPYAVLILLFIGVTFGGIHTW
                     EYTNSPEFCGTACHTMPPQDTVYKESPHANVTCEECHIGRASFLDQAMRKTQGIKETY
                     YEVFKLYEYPIRAKALRPSLDTCEKCHRPETFSDDSLRKIDHFASDRENTASSVYLIM
                     KTGGGDAREGNARGIHWHITSKVLYYSDDELSQTIPYVRVYNEDGTFTEYTDIESGFD
                     PTTIDESQLKQMDCVTCHNRVTHNFKEPSKSVDESMSSGLIDPSIPFIRQKAVEALST
                     EYATRDEAMKAIAGIEDEYKRNLFDVYSQNGEKIQQAIVEIQAIYDRTVFHDQKIDWK
                     TYPNNLGHIDAPGCFRCHDGKHLNAENEAVRLECNVCHAVPVTANADDFVTRIEISRG
                     PEPETHLNPNWISMHNQTLGPSCTNCHNTKDPGGTSNTSFCSNSACHGNVFTYAGFDA
                     PALREIIQSQLPPPEPALEVP"
BASE COUNT         1321 a         1408 c         1076 g         1145 t
ORIGIN      
        1 gcactgctgg tgctgagatc aagtatgatg cagccaccta cccatacttc atgggcgccg
       61 atggcaaagc ctaccccaac tggacacccc gcttgctcaa agctgcttac aactaccagg
      121 tttccatgaa ggatcctggt gcgtttgctc acggcaataa atacatcgtt cagttgctct
      181 tcgattcgat caccgacctc ggtggcgata ccagcaaact cgcccgcact gatgctggcc
      241 actttgctgg caacaccatg cccttccgcg attgggattt gaccgaagat ggtcagccta
      301 actacatggt accctttggc tgtgtgaaat gccataccgc ccagggtctg cccaccttca
      361 tcaaagatgg cggcactact gtagtaacct ccagtggaac aacctcaacc actggcgttc
      421 aatccatgcc ttccagcaat ggctttatgt gctccacatg ccataacgaa gaagcgtggc
      481 cagaacgtta tgctgttgct agtgttgtct tccccagtgg caagaccgtg tccttcggcg
      541 gtaaagatgc tgatggcaac tgggtcgctg acgatgccaa tttgtgcatt tcctgccata
      601 tgggccgtga atccacctca agcgtaaata acgccctcaa aggcaaggat ccggacacag
      661 ttgacgccaa gatccgtttc aagaacatcc actactttgc agctggtgct accatcttcg
      721 gcaacgatgc tcaaggtgcg tatcagtacg aaggtaagga atatcttggc cagaatatgc
      781 atgcggacga agaaggcaag atgaacaagt gccaggactg tcacgatgtt cacgccctcg
      841 aacccaaggt tgagtcctgc gaaacctgcc acgacaccac tgaccccacc accattcgcg
      901 aaagtgaagt ggattatgat ggtgacggcg atgtggccga aggcatcaag ggtgaagttg
      961 atacactcgc agaagctctc tatgcccaac ttcaaaccta tgctgcggca aacggtggcg
     1021 caatcgaata caaccctgcc gcctacccgt atttctttgg tgctgacgga aaatcctatg
     1081 caacatggac accccgcctg ctccgcgcgg ccttcaacta tcaatatagc cagaaggacc
     1141 caggtgttta cgtacataat ccgaaataca tcatccaaat cttgatcgac tcgatcgagg
     1201 atcttggtgg taccgtgagc acttacacac gcccggaagt tccagctccc gctcagtaag
     1261 acaatagttt ccagcacgaa aaaggatggc ggacagaaat gtccgtcatc ttttttaatc
     1321 ccttattaat cacaccaaaa cagtgatatt cagcactata tccctgtgat gacctctcgt
     1381 acaatatggg gcaatcgccc catgacgcat tttattcagg cgtataatcg gacaaaattt
     1441 cctttaatag gagaatgtga tgaattttag aaaatcgctc cgcccgcatc atctttggac
     1501 cgtgagtctg ctctttggaa ttgggttttt tcttttcgcc ctcatcgctc tcacaacccc
     1561 cgcccaggct gctccatccc accaagacga taaaccttca aatgatttct gtcttgcctg
     1621 ccatcaagaa gaggggatca agaaaacatt gggcagcgaa tcactccccg tcaccattaa
     1681 ccccactcga tttgggctat cagttcacgg tgaagaggga atcgcctgcg ttgattgtca
     1741 ccaggacatc agcgattatc cccatcctga cgtcaaagta aaaaataccc gcgatttcac
     1801 gttggaattc cttgatacct gcaaagaatg tcacgaagat caatacaaca aggcccacga
     1861 cagcgtacat caacgtgctt ttgatgaagg caacaaagat gcagctgtct gtatggattg
     1921 tcataacccg catactcaaa cccgcctgac cggcaaaacc agcggggaaa tcaccccttc
     1981 tgctcgtgtg gaaatccccg aaacctgcgc acagtgccac agcaccattt acgatcaata
     2041 caaaacaagc attcatggta acgcattgac agaggaaggc aacaccgatg tgccaacctg
     2101 tattgattgt catggcgtgc ataacattca agaccccacc actgttacgt tccgaaacag
     2161 cacccccttc ctgtgtgcca actgccacac tgatgccaag atcatggatc aatatggcat
     2221 ctctacgaac gtgcttaatt cctatgtggc agatttccac ggcaccaccg tcaaactctt
     2281 cgaagaggaa ttccccggcc agcccaccaa caaacccgtc tgcaccgact gccacggtgt
     2341 acataacatc tcgaaagtgg acgatcctga tactggcatc gccctgaaag acaatcttct
     2401 tgaaaaatgc cagcgctgtc atccagatgc cacctcaaac ttccccgatg cctggatgag
     2461 ccattatgaa cccagcccgg agaatttccc gcttgtttac tacgtcaacc ttttctataa
     2521 attcttcatt cccgccgtat tgggaggaat gatcctcttc atcattacag atatttaccg
     2581 acgtatcata aatcgtgtca aaggagtcaa gcactcatga gccaatcctc tgccaatctc
     2641 tacatgcgct tccctgttgc gcgccgaatc gaacactggg tgatgatgct ttcattcacc
     2701 accctaggac tcacagggct cgctcaaaag ttttccgacc atccagtagc catttcgatc
     2761 ctgaaggcac ttggcggcat cgaaagcctt cgcaccatcc accacatcgc cgccattgtg
     2821 atgatgtttg gaactgcctg gcacatcctc gtcatgggat atagtgtttt tgtgttgcga
     2881 gaccagatgt ccatgctccc ctccattcaa gatgccaagg atggaattca agcgctgctt
     2941 tataacattg gcatcgccaa aacctatccg caaatgggtc gctacacatt cgaagaaaaa
     3001 atggaatatt gggcctttgt atggggtgcg gcagttatgg gcatcacagg attcctgatg
     3061 tggaatccga tcacagccac tcaatatctt cccggtcaat tcgtgcctgc tgcaaaagcc
     3121 gcacacggcg gcgaagcagt ccttgcggta ttggccatca tcatctggca catgtatggt
     3181 gtccatatca agcgtttcaa caaggccatg ttcaccggaa cccaaaccga agcagagatg
     3241 cttcatgaac acccgcttga acttgccgac atgaaagctg gcatcgctga tagacgtcct
     3301 gatgatgcga ctctacgcaa acgcaagatg atctactacc cgatcgctgc catcgtgaca
     3361 ctgggaatgc tcgctggaat ctttggcttt atcaactccg aagaaactgc acttaccacc
     3421 atcactccgc tgacagagtc tgaaatatac tcgccgcaac aaacacctca accgtaatca
     3481 gatggaccct gcagctttta ttaaaggtct gcagggtctg aatttttgga ggaacaatga
     3541 aattgcgcca aaggattgca aaattttttc atcccgaccc gggctcacct ttctgggtgc
     3601 tcgttctccc ctatgctgtt ttgatcctgc tatttatcgg ggtcacgttt ggcggcattc
     3661 acacctggga atacaccaac tcgcctgagt tctgtggcac agcctgccac accatgccgc
     3721 cgcaagatac agtttacaaa gaatcaccac atgcaaacgt tacctgtgag gaatgccata
     3781 tcggccgcgc atccttcctt gaccaggcca tgcgcaaaac tcagggtatc aaagaaacct
     3841 attatgaggt gttcaaactt tatgaatacc ccatccgcgc caaggctttg cgcccctctc
     3901 tcgatacctg cgagaaatgc catcgccctg aaaccttctc cgatgacagc ctgcgtaaaa
     3961 ttgatcactt cgccagtgac cgtgaaaata ctgcttcaag cgtgtacctg atcatgaaga
     4021 ctggcggcgg tgatgcccgt gagggaaatg cccgtggaat tcattggcat atcaccagca
     4081 aggttctgta ttactccgat gatgagctaa gccagaccat tccatatgta cgtgtgtata
     4141 acgaagatgg caccttcact gaatacactg acatcgaatc aggttttgac ccaaccacca
     4201 ttgatgagag ccaactcaag caaatggatt gcgtgacctg tcataatcgt gtcacccaca
     4261 atttcaagga accctccaaa tctgtggatg aatcaatgtc cagcggtttg atcgacccgt
     4321 ccattccctt catccgccag aaggcggttg aagcactctc cacagaatac gccacacgag
     4381 atgaggcaat gaaagccatt gctggcattg aagacgaata caaacgcaat ctattcgatg
     4441 tgtacagcca gaatggcgaa aagattcaac aagccattgt tgaaattcaa gccatttatg
     4501 accgcaccgt cttccatgac caaaagatcg actggaaaac ctacccaaac aacctcggcc
     4561 atattgatgc accgggctgt ttccgctgcc acgatggcaa gcatctgaat gcggaaaatg
     4621 aggctgtacg ccttgaatgt aatgtatgcc atgccgttcc agtgaccgcc aatgccgatg
     4681 actttgtcac ccgcattgaa atcagccgtg gaccagagcc cgaaacccac ctcaacccga
     4741 actggatcag catgcataac cagactcttg gcccgtcatg cacaaactgc cacaacacaa
     4801 aagacccggg cggcacaagc aacacatcat tctgttccaa cagcgcctgc catgggaatg
     4861 tgttcacgta tgccggtttc gatgcacccg ccctgcgtga aatcattcag agccaattgc
     4921 ccccgccaga acccgcattg gaagtacctg
//
LOCUS       DAOQFT010000121         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_8995_length_4905_cov_5.301856, whole genome shotgun sequence.
ACCESSION   DAOQFT010000121 DAOQFT010000000
VERSION     DAOQFT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8995_length_4905_cov_5.301856"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1816)
                     /gene="lepA"
                     /locus_tag="PLR93_04410"
     CDS             complement(17..1816)
                     /gene="lepA"
                     /locus_tag="PLR93_04410"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNH26236.1"
                     /translation="MTDHIRNFCIIAHVDHGKSTLADRLLQLTGTISERDMTEQTLDS
                     MDLEREKGVTIKASAVRMFYSATDGKRYELNLIDTPGHVDFGYEVSRALKACEGAILV
                     VDATQGIEAQTLANLYQALDADLEIVPVINKIDLPSARPDEVAEDVGSLLGVDPEAVL
                     RVSAKEGLNMDQVLEAIVQRVPPPKDVDNEPLRALVFDAHYDSYKGVVAYVRVFEGRI
                     KANEVLRMFSTKNDLRPVEIGIFSPGMKPVESIGSGEVGYVATGFKTVHECRVGDTLI
                     SASAPAAEPLPGYFLPKPMVFAGIYPVEADDYTELRESLEKLQLNDASLTYQPETSQA
                     LGFGFRAGFLGLFHMEIIQERLEREYLLDVLFTAPSVEYEVLTISDEVVKVDSPAELP
                     DPSKVIEVREPWMNIEIITPTDYYGPIMDLVTKRRGIFKQQEHPAPHRVQLDFEIPLS
                     EIIVDFFDHLKSRTKGYASLDYQFLEYRADNLQKLEILVNGDTVDALAAIVHEKDAYH
                     KGQRLITKLKDLIPRQLYDVAIQAASGGRIISRANVKATRKDVLAKCYGGDISRKKKL
                     LEKQKRGKKRLKMVGNVEIPQDAFMAVLKLEDE"
     gene            2028..2726
                     /locus_tag="PLR93_04415"
     CDS             2028..2726
                     /locus_tag="PLR93_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26237.1"
                     /translation="MKNQTFPLYLILYVVGAVLGLALTFFATWADLEAAYYGFDRTGG
                     QRLSSLSCPILMTRTETSKISIKFTNSTERKISPSIKVDLSSPINTISTIEEVQLAVG
                     ESKRISWEIGPENIDLGRFVFARVWTYASYPIKDKESTCGVFVLPLPGSGVFYTWIMV
                     FGCLFGVGYGLMKMRQVKGPGRSGPYERYSFLAIVTVLGLIVSILGWWLAGVLVIVLA
                     LLVSVISMGYSFKL"
     gene            2797..4773
                     /locus_tag="PLR93_04420"
     CDS             2797..4773
                     /locus_tag="PLR93_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH26238.1"
                     /translation="MQILGTICVGGLGVLILVLLTGIRFIPNNRVGIIEKRFGFKPLK
                     SGLIALNGETGFQPQVLRGGLHYLMPVQYAVHMAPLVTITQGKIGYVFARDGLPLEPA
                     QVLASNVTANDFQDVMSFLKNGGQKGPQRLILREGTYAFNLMQFVVITEERVYSLPLS
                     RDDTAVIVRMAEVIKERDGFRPVIIKDNDDKIGIVTVHDGPSLPQGEIIAPVVGDNPE
                     KADEYHNKFQDADRFLKANGMRGRQLQVLVEGTYYVNRLFATVEMIQKTIIDVGNVGV
                     VVSYTGEQGADLSGSEYRHGELVTRGQRGVWSEALLPGKYPFNTYAGKVIPVPTTNFI
                     LKWIKNEVGSHNFDENLSEVALITKDAFEPTLPLSVVVHIDYKKAPLVIQRFGDIKKL
                     VEQTLDPMVAAYFKNVAQTRTLIQLLQERSAIQQQSSLEMKTKFEHYNLELEEVLIGT
                     PSSPAGDKQIETILTQLRQRQVAEEQVETYSQQEKAAVKERELREAESRAAMQGKMTE
                     AELSITIQSNQGKAEYERSIQQAQQIRALAEAEAEKVARIGIAQALATEEQVRAYGGP
                     QFQVTQQVLNRFAEAIQQSGVDVVPRVVVGGGNGEKGGSTQSNIMEGLLTMLLSERFG
                     VSADAGVTQRSPDVEAMREKILKDMQKKDDKKKE"
BASE COUNT         1208 a         1204 c         1304 g         1189 t
ORIGIN      
        1 cgacattgcg taataattat tcgtcttcca atttcaagac ggccatgaag gcgtcctgcg
       61 ggatttccac atttcccacc atcttcaaac gcttcttgcc tcgtttttgt ttctcaagca
      121 attttttctt gcgcgaaata tcgccgccat aacactttgc cagcacatcc ttgcgggtgg
      181 ccttaacatt ggcgcggctg atgatgcgcc ctccagaagc ggcttgaatg gcaacatcat
      241 acaattgacg cggaattaaa tccttgagtt tggtgatcaa ccgctggcct ttatgatacg
      301 catccttctc atggacaatg gccgccaatg cgtcgaccgt atctccattg acgaggatct
      361 ccagcttctg caaattatcg gcgcgatatt caaggaactg ataatcaagc gaagcatagc
      421 ccttggtgcg tgactttaag tgatcaaaga aatccacgat gatctcggaa agcggaattt
      481 caaaatccaa ctgcacgcgg tgcggggcgg gatgttcctg ctgtttgaag ataccacgcc
      541 gtttggttac gaggtccata atcgggccat aataatcggt gggggtgatg atctcgatgt
      601 tcatccacgg ctcgcgtact tcgatcacct tgctgggatc gggcagttcg gcgggagagt
      661 ctacttttac gacttcgtct gaaatcgtca gcacttcata ttccaccgaa ggcgctgtga
      721 acaacacatc cagcaaatat tcgcgctcga ggcgttcctg aatgatctcc atatggaata
      781 gtcccaggaa tccggcacgg aacccgaacc ccaatgcctg cgaggtttcg ggttgatatg
      841 ttaatgatgc atcgttaagt tgcagttttt ccagtgattc acgcagctcg gtgtaatcgt
      901 ccgcttcgac gggataaata cccgcaaaga ccattggctt gggcaaaaaa tatccgggta
      961 agggttctgc cgcgggggca gaagcagata tcagcgtatc gccgacgcgg cattcatgaa
     1021 cggttttgaa tccggtggcg acgtatccca cttccccgga gccgatactt tccacgggtt
     1081 tcatgcccgg cgaaaaaatg ccaatctcca ccggtcgcaa atcgtttttg gtggagaaca
     1141 tgcgcagaac ctcattcgct ttaatgcgcc cttcaaaaac gcgcacatag gcaaccaccc
     1201 ctttatacga atcataatga gcatcgaaga caagcgcgcg caagggttca ttgtccacat
     1261 cttttggagg gggaacacgc tgaacgatcg cctcaaggac ttgatccatg ttaaggcctt
     1321 ccttcgcgga gacgcgcaaa accgcctccg gatcgacccc gagcaggctg ccaacatcct
     1381 cggccacttc atcgggacgg gcggaaggca gatcgatctt gttgatgaca ggcacgatct
     1441 ccaggtcggc atccagagct tgatagaggt tcgccaatgt ttgtgcttca atcccctggg
     1501 tggcatccac caccaggatg gcgccttcac aggctttcaa agcacggctg acttcataac
     1561 caaagtccac atggccgggg gtatcaataa gattcaattc gtaacgtttg ccgtctgtgg
     1621 cagaatagaa catacgcaca gccgaggcct tgatggtgac gcctttctcg cgttcaaggt
     1681 ccatcgagtc gagcgtttgt tcggtcatgt cgcgctcgga gatggtgccg gtcagttgca
     1741 gaagccggtc agccagcgtg gatttaccat ggtcaacatg ggcaatgatg cagaaatttc
     1801 gaatatggtc ggtcatgctc tttcagtgat tctcttccaa aaagtacgta tagggcgttt
     1861 aagcgaacga agtataaccg attaattcag cgcgtccttc catgccgaat tcaaggtaca
     1921 aatcccattc cccatccatt aatgggagaa aaacagttct gctgtattgg attgaatcat
     1981 tcgagagagg atacaatttt tgcacaatcc attcaagaga agtagttatg aaaaaccaga
     2041 cattcccctt atatctcatt ttatatgttg ttggcgccgt tcttgggctg gcactgacct
     2101 tttttgccac ctgggccgac ctcgaggctg cctactatgg ttttgaccgt accggcggcc
     2161 agcgtttgag ttccctgagc tgtccgattt tgatgacccg cacggagacc tcaaaaattt
     2221 cgatcaagtt tacgaactca accgaaagaa agatatcccc atccataaaa gtagacctta
     2281 gttctcccat taataccatt tcgaccattg aggaagtaca actcgcagtt ggggaaagca
     2341 aaagaataag ctgggagatc gggccagaga atatcgatct gggcaggttt gtctttgccc
     2401 gtgtttggac atatgcctct taccccataa aggataagga gagcacttgt ggtgtgtttg
     2461 tccttccgct gccgggcagt ggagttttct atacatggat catggtcttt gggtgtctgt
     2521 tcggggtggg ctatggcctg atgaagatga ggcaggtcaa agggccgggg cgaagcgggc
     2581 cgtatgaacg ctatagcttt ctggccattg tcacagttct ggggctgatc gtcagcattt
     2641 tgggatggtg gctggcgggg gttttggtga tcgttctggc gcttttggtc tcggtgatct
     2701 ccatggggta ttcgttcaaa ctgtgacttc ggtacgggct tacaaggccg aaacctggtt
     2761 gtacaatgga cgtatattcc atacgaagga gcaaccatgc agattcttgg aacaatttgt
     2821 gtaggtgggt tgggagtgct gattctggtt ctgttgacgg gtattcgttt catccccaac
     2881 aaccgggttg gcattatcga aaaacgattt gggtttaaac ccttaaaatc gggcctcatt
     2941 gccctaaacg gtgagactgg ttttcaaccg caggtgttac gcggtggttt gcattatcta
     3001 atgccggttc agtatgccgt gcatatggcg ccattggtaa cgatcaccca ggggaagatc
     3061 ggatatgtgt tcgcccgcga cggtctgcca ttggaacctg cacaggtctt ggcatccaat
     3121 gtgactgcca atgattttca ggatgtcatg agcttcttga agaacggcgg acagaaaggt
     3181 ccacagcgtt taattttgcg cgaaggtacc tatgccttca acctgatgca gttcgtggtg
     3241 atcactgaag agcgtgtgta ctcactgccg ctcagccgtg acgataccgc cgtcatcgta
     3301 cgcatggcag aagtgatcaa agaacgtgat gggttccgcc cggtcattat caaggataac
     3361 gatgacaaga tcgggattgt gaccgtgcat gacggtccgt ctttgccgca gggcgagatc
     3421 attgctccag tcgtaggtga taaccctgaa aaagcagatg aataccataa caaattccaa
     3481 gatgctgacc gcttcttgaa agcgaacggt atgcgaggtc gccagttgca ggtgttggtc
     3541 gaaggcacct attatgtcaa ccgcttgttt gcaaccgttg agatgatcca aaagaccatc
     3601 attgacgtgg gcaatgtggg tgtggttgtt tcctatacag gcgagcaagg ggcggacctc
     3661 tccggctcgg aataccgaca tggtgaattg gttacccgcg gacagcgcgg tgtttggagt
     3721 gaagcactct tgccgggtaa gtatccgttc aacacgtatg caggcaaggt tatccctgtg
     3781 ccgaccacca acttcatttt gaagtggatc aagaacgaag tcggctcaca caactttgat
     3841 gagaacttga gcgaagtggc gctcatcacc aaggatgcgt ttgagcccac acttccactt
     3901 tctgtggtgg ttcatatcga ctataagaaa gctccattag tcattcaacg cttcggcgac
     3961 attaagaaac tggtagagca aaccctcgac ccgatggtgg cagcatactt caagaacgtt
     4021 gcgcagactc gcactcttat tcaactcttg caggaacgca gcgccatcca gcagcaatcc
     4081 agcctggaga tgaagaccaa gtttgagcac tataatcttg aactggaaga agtgctgatc
     4141 ggaacgccat cctcccctgc tggtgataag caaatcgaaa ccatcctgac ccagttgcgt
     4201 caacgccagg tggcagagga acaggttgag acctactcgc aacaagagaa ggctgcggtc
     4261 aaagaacgtg aactgcgtga agccgaatcg cgtgctgcca tgcagggcaa aatgaccgaa
     4321 gcggagttga gcattactat ccagtcgaac cagggtaaag cggagtacga gcgctcgatc
     4381 cagcaggcgc agcagattcg cgctttagca gaagcggaag ccgagaaggt cgcccgcatc
     4441 ggtatcgcgc aagcgctcgc taccgaagaa caagtgcgtg cgtatggcgg tccgcagttc
     4501 caggtgaccc agcaagtgct caaccgcttt gcagaagcca ttcaacaatc aggcgtggac
     4561 gtggttccac gtgtggtggt gggtggcggc aatggtgaga agggcggcag tacgcagtcc
     4621 aacatcatgg aaggcttgct gaccatgctg ctctccgaac gctttggtgt ctctgcggac
     4681 gcaggagtca cgcaacgctc acccgatgtg gaagcgatgc gcgaaaagat cctgaaagac
     4741 atgcagaaga aagacgataa gaagaaagag taagttgaat caaaagaggc tggcgataac
     4801 tcgccagcct cttttttatt acaagtcatc tcaaaaatac agtgtcaccc tgagggagcg
     4861 ttcttcgcga ccgaagggtc tcttacttta ggcgtagaga tcctt
//
LOCUS       DAOQFT010000122         4893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9030_length_4893_cov_5.257751, whole genome shotgun sequence.
ACCESSION   DAOQFT010000122 DAOQFT010000000
VERSION     DAOQFT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9030_length_4893_cov_5.257751"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLR93_04425"
     CDS             complement(<1..526)
                     /locus_tag="PLR93_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNH26239.1"
                     /translation="MEISQITVWLALLAGLASFLSPCVFSLVPAYVGYLGGIAAGSGT
                     PGEKPNRWLTFSHGVAFVLGFSIVFVLLGVAASFAGGLLYDLRYWLAKIGGVVVIIFG
                     LHMIGVFHIPFLAYDTRVQKAPDPKLGYFSSALMGVFFSAGWSPCVGPVLGAILTLAI
                     NGGSISQGATLLTFY"
     gene            complement(536..1702)
                     /locus_tag="PLR93_04430"
     CDS             complement(536..1702)
                     /locus_tag="PLR93_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNH26240.1"
                     /translation="MRKYLSRRDFIKLAGLGVGAMAFTRLSPSDVDYRWEIFSQPKRL
                     PQFPGSAIIGRVCEPDIDLRSSPTNNPNLNNSLSKLNADTLVEWNREVIGNVIGGLPN
                     QRYVETPQGYVYASVVQPTKNIPNTPVTELPNGQGFWAEVTVPYVELAHEGTVASPWL
                     QDHITYNFPPRLYYGQVVWIDQIRTTNGYPEYRWNEDAAGRGYGYGGGYGEFYWADGA
                     GLKILTDADISTINPEVDPNEKTISIDLDYQTLSCYEGAREVYFCRISSGKKYDPVTG
                     QVTDAYATPAGSLLTYWKIISKNMTAGNEGSGYSTPAVPWCTFIASGGVAIHGAFWHN
                     AFGERRSHGCVNVTPEDSKWIFRWTMPYVSLAAGEERRELPDHGTTVTSTETQF"
     gene            complement(1786..2463)
                     /locus_tag="PLR93_04435"
     CDS             complement(1786..2463)
                     /locus_tag="PLR93_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26241.1"
                     /translation="MQTQTNETIEFNGWTMRYRSAETSHPRLLVMIHGLTGDENSMWV
                     FARRFSPNYWIIAPRAPHPANPQGFSWRANPLSIDEARANQTNRPRLDALKEAAEGLI
                     RLIEEYAASVKVEAGIFDAIGFSQGAAMVNVLGMLYPNRVRKMGILAGFAPHGLDEYA
                     SQRVLAGKQVFVAHGTQDDMVPIERARESMKLLEQAGASITYCEDEVGHKLSSNCLRA
                     LEQYLTD"
     gene            complement(2435..2674)
                     /locus_tag="PLR93_04440"
     CDS             complement(2435..2674)
                     /locus_tag="PLR93_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26242.1"
                     /translation="MTRDLRKYMRDTNTRIVVGAILMLFIIGLGLIWLIYGFGAAVTG
                     FLCIAAAFIPIGLVFLFLFGMDWFVKRANPDERND"
     gene            complement(2677..3555)
                     /locus_tag="PLR93_04445"
     CDS             complement(2677..3555)
                     /locus_tag="PLR93_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH26243.1"
                     /translation="MEITLALGGGGAKGNAHIGVLRRLEKEGYKIRSVAGTSFGGMVA
                     ALYAIGYSPREIQAIFESVDQESLYGRASQDGPSLLGMAGVRQLLERVIGEKTFKDTR
                     IPCAVTAVDINTGGEVVLNEGSIKNAVLATIALPGIFPVQKINDWELVDGGVLNPVPV
                     SVARLLSPDLPVVAVVLNDPIDKPVRGYNLPLPSIVPRTIAERLARISLAQSLDIFLR
                     SVDVSSRYVSHLRLQVDKPEIIVRPDVHHIELLDQVVVEDVAALGEQALEQVLPDLKR
                     KTTWAARLGRRVFGAN"
     gene            complement(3658..4671)
                     /locus_tag="PLR93_04450"
     CDS             complement(3658..4671)
                     /locus_tag="PLR93_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNH26244.1"
                     /translation="MRKRISFLFALIILLVLSITPKQTVEAKPFANITSPSQLIDAVN
                     SLRVANGLSPLPVHQALMQSAQSQADYMAATGNVTHSRPSGTYTQQLLALGFPLAGDL
                     SAGGFRSENILSTYGPLVWNGVPSAWQDAQHMNTMLSGNFTHIGAGVSQSGDMYYYTV
                     DTAAATSSGQQQDSAATVLTSVPGGSDAGVSQFMVPVTLNTPRPDGNVYHKVQYGQSL
                     WSIAIAYGTTIKNIQALNNLGEASTVYQGQELLVVTGATQAVAPTLDATATTQATATL
                     APTSTNVPTSTPELVLLTPTEEAPVEANTKPASSRVLIVVLIVAAFVGAGMAVWLIRD
                     PGN"
     gene            complement(4680..>4893)
                     /locus_tag="PLR93_04455"
     CDS             complement(4680..>4893)
                     /locus_tag="PLR93_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNH26245.1"
                     /translation="PEPWFAQMAVATLPADSDLVALKSRLYDEHRIEIPLIAWNEKKL
                     IRLSVQGYNSRRDMDKLLSALNTLLR"
BASE COUNT         1350 a         1289 c         1152 g         1102 t
ORIGIN      
        1 aatagaaagt aagaagcgtg gcgccctgag agatcgagcc gccgttaatg gcgagagtca
       61 aaatcgcacc gaggacaggt cctacacagg gagaccatcc ggcagagaag aatacgccca
      121 tcaatgcgga cgaaaaatat ccgagcttgg ggtctggtgc tttttgaaca cgggtgtcat
      181 acgcaaggaa cggaatgtga aagacaccga tcatgtgcaa accgaagatg atgacgacca
      241 cgccaccgat ctttgccaac caatagcgta aatcgtataa caaaccgcct gcaaaagaag
      301 ccgctacacc gagcaacacg aacacgatac tgaaccctag tacgaaagca actccatgtg
      361 aaaaggtaag ccaacgattg ggcttttcac ccggagttcc actgcctgct gcaatgccgc
      421 caaggtatcc cacataggct gggacaagag aaaatacaca aggggaaagg aaagaagcca
      481 acccggcaag caaagcaagc caaacagtaa tttgggaaat ttccatggac gcctcttaga
      541 attgggtttc ggtagaagtg actgtggtgc cgtggtctgg cagttcgcgg cgttcttcgc
      601 cagcggcaag agaaacgtat ggcatcgtcc aacggaaaat ccacttggaa tcttcaggcg
      661 ttacattgac acagccatgc gagcgacgtt caccaaaggc gttatgccag aaggcaccat
      721 gaatggcgac cccgcccgaa gcgataaaag tacaccaagg cactgccggg gtggaatagc
      781 cagaaccttc attgcctgcc gtcatgttct tggagataat tttccaatag gtcagcaagg
      841 agccagccgg ggtggcatat gcatcagtga cctgccctgt gacggggtca tatttcttgc
      901 cagaagaaat gcggcagaag tacacctcac gtgcaccttc ataacaggaa agagtttgat
      961 agtccaagtc gatggagatg gtcttttcgt tcgggtccac ttcggggtta atggtggaaa
     1021 tatcagcatc ggtgagaatc ttcaacccag caccatctgc ccaataaaac tcaccatacc
     1081 caccgccgta gccatagcca cgaccagcag catcttcatt ccagcgatat tcagggtaac
     1141 cattagtagt acgaatctgg tcgatccaga ccacttgccc ataatacaaa cgcggcggga
     1201 agttatacgt aatatggtct tgcaaccaag gcgaagcgac agtcccttcg tgagccaact
     1261 caacataagg aacagttact tctgcccaga agccttgtcc attcgggagt tcggtgacag
     1321 gcgtattagg aatgttcttg gtcggctgaa caacagaggc atagacatag ccctgcggcg
     1381 tttccacata gcgttggttt ggcaacccac cgatgacatt gcctatcact tctcgattcc
     1441 actctacgag agtgtcggca ttcaacttac tcagtgaatt attcaaattc gggttattgg
     1501 ttgggctgct gcgcaaatca atatcgggtt cacacacacg tccaatgatg gcactgcccg
     1561 ggaactgtgg taaacgcttg ggctggctaa agatctccca ccgataatca acatcactcg
     1621 gagatagacg cgtaaatgcc atggcaccca cgcccaaacc agccaacttt ataaaatcgc
     1681 gtcgcgataa atatttcctc atagtttcac ctttatatag aagagattac gtcaacaagc
     1741 atacctgcaa ataaaaacag cgtcaacagc gcggtgagaa acagattaat ctgtaaggta
     1801 ctgctccagc gcccgcaaac aattcgaact caatttatgc cctacttcat cttcacaata
     1861 tgtgatcgaa gcccccgctt gctccagcaa tttcatcgac tcacgcgccc gctcaatcgg
     1921 caccatatcg tcttgtgtgc catgcgccac aaaaacctgc ttacccgcca aaaccctttg
     1981 gctcgcatat tcgtccaagc catgaggggc aaacccagcc aaaatgccca tttttcgaac
     2041 acggttcgga tacaacatcc ccaacacatt gaccattgcc gcaccctgac taaacccaat
     2101 cgcatcgaag atgcccgcct ccaccttaac cgaagctgca tactcctcaa taagccttat
     2161 caacccttca gccgcttcct tcagcgcatc caaccttggg cggtttgttt gattcgcacg
     2221 cgcttcgtca atgctaagcg gatttgcacg ccatgaaaaa ccctgcgggt tagcagggtg
     2281 tggcgcacgt ggagcgatga tccaataatt tggagaaaaa cggcgcgcaa aaacccacat
     2341 ggagttttcg tcgccggtca aaccatggat catcaccaac aaacgcggat gcgaggtttc
     2401 ggctgagcga taacgcatcg tccaaccgtt aaattcaatc gtttcgttcg tctgggtttg
     2461 cacgttttac gaaccaatcc attccaaata aaaataaaaa gaccaaacca atcgggatga
     2521 atgccgcggc aatacagaga aaacctgtca ctgctgcgcc aaagccgtag atcaaccaga
     2581 tcagccccag cccgatgata aacaacatca gaatcgcgcc gacgacgatc cgcgtattgg
     2641 tgtcacgcat gtacttgcgc aggtcacgag tcattgttaa tttgctccaa acacacgccg
     2701 acccaagcgg gcagcccatg tggttttgcg cttcaggtct ggcaatactt gctctaatgc
     2761 ctgctcgcct aaggcagcca catcttccac aacaacctga tctaacaatt caatatggtg
     2821 cacatccggg cggacaatga tctcgggctt atccacttgc aagcgcaaat gcgaaacata
     2881 gcggctggaa acatctacag agcgcaagaa gatatccaag gactgggcta acgaaatcct
     2941 tgcgagccgt tcagcaatcg tacgtggaac aatgctgggc agcggcaggt tatatccgcg
     3001 cacgggttta tcaatggggt cgttcaacac aacggcaacc accggcaggt ctggcgataa
     3061 gagccgagcc acagacaccg gcaccggatt caagacgccg ccatccacta gttcccaatc
     3121 gttgattttc tgcaccggga agatacccgg caaagcgatt gtggcgagta cagcattttt
     3181 gatactgcct tcattaagta ccacctcacc gcccgtgttg atatccacgg cagtgacggc
     3241 acaaggaatg cgagtgtctt tgaaggtttt ctcaccgatc acacgttcta acagttgacg
     3301 aaccccagcc attcccaata gagacggacc atcttgcgag gcacgcccat aaaggctttc
     3361 ctggtctact gattcaaaga ttgcttgaat ttctcgcgga gagtagccaa tagcatataa
     3421 agcagcgacc attccaccaa aactagtccc tgcgacagag cgaattttat atccttcttt
     3481 ttcaagacga cgtaacacgc caatgtgggc attgcccttg gcgcccccac cgcctaatgc
     3541 caacgtaatt tccattttct tcgatgcctt tcaatattca attaacggac aaatcaaacg
     3601 cgagggatta tacccttaca aaaaaacaac atagtttcaa cacattttct gagaaactta
     3661 attccccggg tcgcgaatca gccaaactgc cattcccgcc cctacaaatg ccgccacaat
     3721 caaaacgaca atcaacactc ttgaagaggc tggctttgta tttgcttcaa cgggtgcttc
     3781 ttctgtaggg gtaagcagta ccagttctgg ggttgaggta ggaacgttgg ttgaagttgg
     3841 agccaaggtt gccgtggctt gtgtcgtagc tgtggcatcc aaagttggag ccactgcttg
     3901 agtggcgccc gtaactacga gcaactcttg cccttgatat acggttgagg cttcgccaag
     3961 gttgtttagc gcttgaatgt tcttgatggt tgtgccatag gcaatggcaa tgctccacaa
     4021 cgattgtcca tactgcacct tatgatacac atttccgtcg ggacgaggcg tattcaatgt
     4081 gaccggcacc atgaactggc tgacacctgc atcactgccg cccggcacgc tggtgagaac
     4141 cgttgcagca ctatcttgtt gttgcccaga gctggttgca gcggcggtat ccacagtgta
     4201 ataatacata tcgcctgatt gtgaaacgcc agcgccaatg tgtgtaaagt tgcccgaaag
     4261 catggtgttc atatgttgag catcttgcca ggcgctcgga acaccattcc atactaaagg
     4321 tccgtaggta cttaagatat tttcggagcg aaagccgccc gcacttagat cgccggctaa
     4381 cggaaagccg agcgcaagca attgctgggt gtaggtgccg ctggggcgag agtgtgttac
     4441 attgcctgta gctgccatgt aatctgcttg cgattgagca gattgcatca atgcctgatg
     4501 gacaggcaag ggtgaaagcc catttgcgac ccgcaaacta ttgaccgcat cgatcaattg
     4561 ggatggggat gtaatatttg caaatggttt tgcttcaaca gtttgttttg gggtaatgct
     4621 caatacaagc agaataatca aggcgaataa aaacgaaatc cgttttctca tatatgcttt
     4681 catcttaaca acgtattcaa tgcagataat aatttgtcca tatctcgacg agaattatat
     4741 ccctgcacag acaaacggat taatttcttc tcattccacg cgatgagcgg aatttcaata
     4801 cgatgctcat catacaagcg cgatttgagg gcaacgaggt cagagtccgc tggcagagtg
     4861 gcaactgcca tttgggcaaa ccaaggttcg ggg
//
LOCUS       DAOQFT010000123         4881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9058_length_4881_cov_7.197265, whole genome shotgun sequence.
ACCESSION   DAOQFT010000123 DAOQFT010000000
VERSION     DAOQFT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9058_length_4881_cov_7.197265"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLR93_04460"
     CDS             <1..291
                     /locus_tag="PLR93_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNH26246.1"
                     /translation="SLLAIGSGPVLSVLIMLLLAVLLSICSTVDSFVALGFMGTFSFG
                     AVLSFLVFGPMVDIKSTIMYLQVFKRRAVAYIIGIPFLMSLLAGLIFNYFVK"
     gene            324..1073
                     /locus_tag="PLR93_04465"
     CDS             324..1073
                     /locus_tag="PLR93_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HNH26247.1"
                     /translation="MPQRMFRSYQGLLLFGLFIFFVFKAVNGQLTWYINSRFVPLTIA
                     GMIFLGILAQTVFSENRRSRKHEEEHPEHHHEHDHAPSSVTLWVMLVPLLIGILIPAR
                     PLDSSAFSTKGFNTSTPLVRSDSSASIFETESQERDILQWLKLFTFQLDVTKFIGQEA
                     SVVGFVYFDDKLEQNQFFVSRFVVSCCAADAFAIAMPVRWEKSAELQQDSWVVVRGTI
                     DSIDIEGVRTPMIIASAVEATDIPAQPYIYP"
     gene            1070..2431
                     /locus_tag="PLR93_04470"
     CDS             1070..2431
                     /locus_tag="PLR93_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26248.1"
                     /translation="MKTHLSIDTLAVSVMIALTIIIGIVILIGEGAGIQVRVDLPEDG
                     VVGPFEAVTFTFPEKISAEIASDLISLEPIHEGYLSAVDDYSVRFIPLKPYEQGVTYT
                     FKVSPGEINATTREVKKTQSWQFHVREPRVAYVVSEAGQSSIWSMTKLGSNVKRLTDE
                     TIKVISFDVAQSGDFIVFSSANQNGGIDLWRVSREGGDASMLLDCGLDRCTTPVIAPG
                     DKRIAYSREAAGPNPDLPFGSPRIWVVDLESGADQAIYEDQSILGYNPSWSPDANRLA
                     SFDGLADFIHMIDFKTGDQFLFPSTTGGPVTWSPDSNQFLYTTFTQTEEGGRTQIKLA
                     DLTTNETITLIGEKDTYDYAYYSVAWSPLAERAVLSLRADEEKPTQVLWVFDPNLLDG
                     IIVANDPEYTYNSPQWDPWGNALLFQQFKLKGQYRPEIGLWQEGTIQSEVITEGILPQ
                     WLP"
     gene            2505..>3687
                     /locus_tag="PLR93_04475"
     CDS             2505..>3687
                     /locus_tag="PLR93_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26249.1"
                     /translation="MKKQLFKFTAMFMIAFLLLPLRTAKAHPADVYAHTITVNLTQTQ
                     MTIKWQIKPGPLLVNFIWNEMDSNQDGTVSEDEAQTWSASRLDSFLVSLDGKSLPLTV
                     DSINIPSSMQAFQAGEEFIIYNLSADLSQSTNNTQRIIIENGMEAAKSINWFYLTAEE
                     GTAFLFPAQRSHILTIDYIHNPASIDDQSRLLTTWDSGAPALPFGQKKDVVTETAEQV
                     VPELSQRSPQEILLDLVRSKELSLTFYLFALLIALALGALHALTPGHGKTVVAAYLVG
                     SRGTSWHAVVLGTVVTLTHTGSVFLLGILTLAASKYFLPTAVIPFLEILSGALILGLG
                     LYLLWQRFVYWRNSRKPKEPQPKHISLTPKSSAPKPVGSIKVQTMKPGMHHHGDGKMH
                     SH"
     assembly_gap    3688..3787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3788..>4881
                     /locus_tag="PLR93_04480"
     CDS             <3788..>4881
                     /locus_tag="PLR93_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26250.1"
                     /translation="QTMKPGMHHHGDGKMHSHDVPETITWRSLIALGVSGGLVPCPDA
                     IAILLVAIAINRLLLGLALILSFSLGLAVVLIVIGLLMVNSRRLFDRMGFLDRFAPVL
                     PVVSAVIVVILGAALTWGAAVRAKNNVGLALPVQTSVREARILYLRENESRIKQLFIT
                     DYTSDTSSLISEETQSVDDYIVSPDATQAAYITQKDSESSQLWLVDLKTDERKKITDC
                     VQAICSGMVWSPDGSRIIYENMSVNGNGLPTLWWVDLATAEAQPVFQDSNLPGSNPRW
                     SPNSQWLSYATPEGIRLYNLENGETRIIPNVLGAAAMWAPNSKSILLRDVVIKHDQFV
                     TQLFIYGMESEVLVNISPSEGFENTLAAWSP"
BASE COUNT         1143 a         1295 c         1135 g         1208 t
ORIGIN      
        1 tccctgcttg ccattggaag tggaccggtt ctttcggtct tgatcatgct tctgcttgcc
       61 gttctgcttt ccatttgttc gacagtggat tcgttcgtag cgctcggctt tatgggcaca
      121 ttcagctttg gggctgtgct ttcattttta gtgtttggtc ccatggtgga tatcaaaagc
      181 accatcatgt atctgcaagt tttcaaacgt cgcgccgtgg catacatcat tggaattccc
      241 ttcctcatga gtttgctcgc cgggttgatt tttaattatt ttgtgaaata ggacaagcgt
      301 ccatcatcgc ttttggagaa cttatgcctc aacgaatgtt ccgttcgtat caaggattac
      361 tacttttcgg gttgttcatc ttctttgtgt ttaaagccgt caatggacag ttgacgtggt
      421 acatcaactc tcgcttcgtt ccgctgacca ttgccggcat gatcttcctc ggcattctcg
      481 cgcaaaccgt cttcagtgaa aaccgtcgct cgcgcaaaca tgaagaagaa catcctgaac
      541 atcatcacga acatgaccac gcgccatcct cggtcacctt gtgggtgatg ttggtgccgc
      601 tgttgatcgg tattctcatc cctgcccgcc cattggattc atctgcattt tccaccaagg
      661 gcttcaacac ctctacccct ctggtcaggt cagattcatc cgccagcatt ttcgagaccg
      721 aatcgcaaga acgcgacatc ttgcaatggc tcaaactctt tacatttcaa cttgacgtca
      781 ctaagtttat tggacaagaa gcctctgtag ttgggttcgt ttatttcgat gacaaactag
      841 agcaaaacca attcttcgtc agtcgcttcg ttgtctcctg ctgtgcagca gatgctttcg
      901 ctatcgccat gcccgtccgc tgggagaagt ctgcagagtt gcagcaagac tcctgggtgg
      961 tagtcagagg tactatcgat tcgattgata tcgaaggggt acgcactcct atgattatcg
     1021 ctagtgctgt tgaagcaaca gatattcccg ctcagcctta tatctaccca tgaaaactca
     1081 tctttcgatt gatacccttg ccgttagtgt gatgattgcg cttacaatca ttattggcat
     1141 tgtcatttta ataggtgaag gcgctggtat ccaagttcgc gtggacttac cagaagatgg
     1201 tgtcgtcggt cctttcgaag cagtgacttt tactttccca gaaaaaatct ctgcagagat
     1261 tgcatcagac ctaatctctc ttgaaccgat ccacgaaggg tatttatcag ctgtggatga
     1321 ctacagcgta cgtttcattc cacttaaacc ctatgaacaa ggtgtaactt acaccttcaa
     1381 ggttagcccc ggtgagatca acgctaccac acgcgaagtg aaaaaaacac aaagttggca
     1441 gtttcatgtg cgtgagccgc gagtggcata tgtagtttca gaggcagggc agagcagtat
     1501 ctggtcaatg accaagctcg ggagcaatgt aaagcgtctc acagatgaaa cgatcaaggt
     1561 catctcgttc gacgtcgctc aaagtggaga cttcatcgtc tttagttctg caaatcaaaa
     1621 cggcggcatt gacttgtggc gagtgagccg tgaaggcggc gacgcatcca tgctgctcga
     1681 ttgcggactc gaccgttgca cgacgccggt cattgcgccg ggggataaac gcattgccta
     1741 ttcacgtgaa gcggcaggac ccaaccccga tttgccgttt ggttcaccgc gcatctgggt
     1801 tgtggatttg gaaagcggtg cagaccaagc tatctatgaa gaccaaagta ttttgggcta
     1861 caacccatct tggtcgccgg atgcaaaccg cctcgcctct ttcgacgggc tggcagattt
     1921 catccacatg atcgacttta aaacgggaga ccaattcctc ttcccctcca ccactggcgg
     1981 acctgtaaca tggtcgcctg attcgaatca atttctctac accaccttca cccaaactga
     2041 agaaggcggg cgcactcaaa tcaaactcgc agacctgacc accaacgaaa ccataactct
     2101 catcggcgaa aaagacacct atgattatgc ctattattcc gtagcatggt caccgcttgc
     2161 agagcgcgcc gtcctcagct tgcgtgccga tgaagaaaag cccacccaag tgttatgggt
     2221 cttcgaccca aatttgctcg atggcattat cgtcgccaat gacccagagt acacctataa
     2281 ctctccgcaa tgggatcctt ggggcaatgc cttgctcttt cagcagttca aattaaaagg
     2341 gcagtacaga ccagaaatcg gtctttggca agaaggcacc attcaatctg aggtcattac
     2401 cgaaggtatc ttgccgcagt ggttgccgta atctcacacg tcagtgcgag ggcaaaaggc
     2461 aaggtcgccc tcgcattgac ggttatttat taattggagt ctcattgaaa aaacaactct
     2521 tcaaatttac ggcgatgttc atgatcgctt ttcttttatt gccccttcgc actgccaaag
     2581 cgcacccagc agatgtgtac gctcacacca tcacggtcaa cctcacccaa acccagatga
     2641 cgatcaaatg gcagatcaag ccaggtccac tgcttgtcaa ctttatttgg aatgagatgg
     2701 actcaaatca ggatggcacc gtcagcgaag acgaagccca aacctggagc gcctcacgcc
     2761 tcgactcgtt cctggtcagc ctcgatggaa aatctctccc gctgacagtt gattccatca
     2821 acattccatc gtccatgcag gcgtttcaag caggcgaaga attcatcatc tacaacctct
     2881 ctgcagattt aagccagagc accaacaaca cgcaacgcat catcatcgaa aacggcatgg
     2941 aagcggcgaa atctatcaat tggttttatc tcaccgccga agaaggcacc gcctttctct
     3001 tccccgctca acgcagtcac attctgacca ttgactacat ccacaaccct gccagcatcg
     3061 acgaccagag ccgcctgctc accacatggg acagtggcgc ccccgccctg cctttcggac
     3121 agaagaaaga tgtcgtcacc gaaactgccg aacaagtcgt ccctgaactt tcgcagcgtt
     3181 cgccacaaga aattctgctt gaccttgtgc gcagcaaaga actctcgctg accttttatc
     3241 tatttgcctt acttatcgca cttgccctcg gcgccctcca tgcgctgact cccggtcacg
     3301 gcaagacggt ggtcgcggcg tatctggtcg ggtcgcgtgg cacatcatgg catgccgtag
     3361 tgctgggaac agtggtcacg ctgacgcata cgggctcggt gtttttattg ggcattctta
     3421 cattggctgc ctccaagtat tttctgccaa ccgcggttat tccatttctt gaaatccttt
     3481 cgggcgcgtt gattcttggg ttgggtctgt acttgctttg gcaacgcttc gtctattggc
     3541 gcaattcaag aaagccaaaa gaaccacagc ccaagcacat ttcgctgacg ccaaaatcat
     3601 ccgccccaaa accggtgggt tccatcaaag tgcagaccat gaaacccggc atgcaccatc
     3661 acggcgacgg aaagatgcac tcgcatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnncag accatgaaac ccggcatgca ccatcacggc gacggaaaga tgcactcgca
     3841 tgatgtccct gaaaccatca cctggcgttc actcatcgcc ctcggcgtca gcggcggact
     3901 cgtcccctgc ccggatgcga ttgcgatact actcgttgca attgcgatca accgcctctt
     3961 acttggtttg gcgttgatcc tgtcctttag cctaggtctg gctgtggtct tgatcgtcat
     4021 cggcttgttg atggtcaaca gccgccgtct cttcgaccgc atgggcttct tggatcgctt
     4081 cgcacccgta ttgcccgtcg tttcagcagt gatcgtggtc attctcggcg cggcgctgac
     4141 gtggggcgcg gcggttcgag cgaagaacaa tgttgggttg gcgctgcccg ttcaaacgtc
     4201 ggttcgtgag gcgcggattt tatatctgcg tgagaatgaa agccgcatca agcaattgtt
     4261 catcaccgac tacacaagcg acacgtcgag cttgatctca gaagaaaccc aaagcgtgga
     4321 tgattacatc gtttcacccg atgcaactca ggcagcctac atcacgcaga aagatagcga
     4381 gtcgagccag ctttggttgg tggatctaaa aacagacgaa cgcaaaaaaa taaccgattg
     4441 tgtacaagcg atttgttctg gcatggtatg gtcgccagat ggcagccgaa tcatttatga
     4501 aaacatgagc gtgaacggta atggcttgcc aaccttgtgg tgggtagacc ttgcgaccgc
     4561 tgaagcacaa cctgtgttcc aagattcaaa cttgccgggc agtaacccgc gctggtcacc
     4621 gaacagccaa tggttgagct atgccacacc agagggaatc cgtttgtata acttggaaaa
     4681 tggcgagacg cgcatcattc cgaatgtact cggggcagca gcgatgtggg cgccgaacag
     4741 caaatcgatt cttttgcgcg atgttgttat caaacacgat cagttcgtga cccagttatt
     4801 tatatatggc atggagtctg aagtcttggt caacatcagc ccaagcgaag gctttgaaaa
     4861 tacactcgcg gcatggtcac c
//
LOCUS       DAOQFT010000124         4879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9065_length_4879_cov_5.372927, whole genome shotgun sequence.
ACCESSION   DAOQFT010000124 DAOQFT010000000
VERSION     DAOQFT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9065_length_4879_cov_5.372927"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="PLR93_04485"
     CDS             <1..1754
                     /locus_tag="PLR93_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH26251.1"
                     /translation="LLMLLVLTGLNLVIPRIIQNVVDDGLLRGDTIYLVRSALLLFGL
                     GVVSALLGLGNRYASEWVAAHVGYDLRNRMYDHIQYLPFAYHDHAQSGQLISRCIEDV
                     RAIEKFAGSSIPELMRFVLLTLGILGVMLTTNSKLAIISLLPMIPLIVMTGWFGTKIG
                     KLFFEVDQAVGEVSNRLQENVTGVQVVRAFAREDYEIKRFESANMDVYHKWLHVINEW
                     AKIMPTTNWLITIGTILILYFGGQMVISGELTVGAVVAFNAYLLMMAEPAQALTGLVN
                     AGGEAAAGAQRVFEVLDTEPEIRSKKEALVLSELKGEVEFHNVSLKYQDENTRSLANI
                     NVKVERNKIVALIGPTGSGKTSLVNLIPRFYDVTDGMVLVDGQDVRDVDLTSLRRQIG
                     IVLQTSLLFSDTIRANIAYGRPDAKQEEIVAAAKAAQAHEFIEGFTKGYDTIVGERGV
                     TLSGGQRQRVAIARALLMNPRILILDDSLSSVDTQTEKLIQAALDVLMEGRTTFVIAH
                     RLSTVRRADMILVMDKGRIVEQGTHDELIKAGGLYKEIHDLQLVEREDVITAVQPIFG
                     EKPLHENTIYSGKSNSG"
     gene            1759..3555
                     /locus_tag="PLR93_04490"
     CDS             1759..3555
                     /locus_tag="PLR93_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH26252.1"
                     /translation="MTTKIIPPEFITKSEEQLDKGYDPKVASGLMKFVKPYYGQTIFA
                     LILMVIVTAASVSGPYFVKRAIDEGITAKDPVALRNITLTFLVVSVIQLGLNYWRVRI
                     MARVGQHVLYDVRTAMFAHLQKLSLSFYNRYSVGRVITRVINDVGTLREFITWAVLAI
                     VRNVLGIVGTLVAMIALNFQLSLLTFIVLPIMVWATIAFRKAARQNYRKVRAAVSWGN
                     SVLAENVNGVRVVQAFSRQQHNYNTFSGYVNRYYLETSLDAVRVASLFTPVIDLLGAL
                     ATALVIYVGGTAVLGESITAGVLIAFVLYIDRFFEPIRDLSRRFDTLQSTMAGGERIL
                     ELLHTPIEVRDEEHANEMGPITGTVRFDNVSFHYSDDPVLVLDSIQLDVKPGETVALV
                     GETGAGKTTIVKLLARFHDPTSGGVLIDGVDLRTVTQGSLRAQMGMVLQDPFLFNGTV
                     KENILFGRLDAKDDEVIAAAKAVGAHDFIMNLKNGYDTSVEEGGLTLSVGQRQLISFA
                     RALLANPRILILDEATSSVDTQTEHVIQTALATLLQGRTSFVIAHRLSTITNADKIVV
                     IDGGKITEQGKHSELIALQGRYYELYKTGFQE"
     gene            3559..4248
                     /locus_tag="PLR93_04495"
     CDS             3559..4248
                     /locus_tag="PLR93_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH26253.1"
                     /translation="MTEPTNEFLYKMSKHMRDGLADVQKKINDLYKIEELEEYVDIPD
                     SPIEHLKAAVMPLVIQVNDFMDYAETVTGRMELDLQDVDVHALLEGVLMMADQLVEMK
                     GGITLEEDIPDNLPTIEGDPTRLSQVLLNYIHNAVKFTEKGTVTVRVARETDKLLFEV
                     SDTGIGIAEENQEKVFEPFETILEDKDDPRLGFGIGLKIVEHIVDLHDGETWFESKKG
                     AGSSFFFTVPL"
     gene            complement(4290..4820)
                     /locus_tag="PLR93_04500"
     CDS             complement(4290..4820)
                     /locus_tag="PLR93_04500"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26254.1"
                     /translation="MIHPHSIRKPQVCVLGSAEPGSTAYDLAGEAGAFLARHGVTLVS
                     GCGSPATRFAAESAIKAGGLVLSIIPSDEMPASDWPGTVVLPSGIGDARNLIMALAGD
                     ACLVIGGRAGTISEVSLAWLHKRPLLPLVGHGGWSNNLPSNPPDERKNSEILPWSSVS
                     ELEHQLQHLGLLLMNK"
BASE COUNT         1225 a          968 c         1320 g         1366 t
ORIGIN      
        1 gcctgcttat gttgttggtt ctcaccggat tgaacctggt tattccgcgt attatccaaa
       61 atgtagtaga tgatggactt ttgcgtggcg atacgattta tctggtccgg tctgcgcttc
      121 tcttgttcgg acttggagtt gtttcagcgc tgctagggtt gggcaaccgt tatgcttctg
      181 agtgggtggc agcgcatgtt ggttacgatc tgcgcaaccg catgtatgac catattcaat
      241 atttgccctt tgcctatcat gaccatgctc aatctggaca attgatttcg cgttgtattg
      301 aagatgtgcg tgcgattgaa aaatttgcag ggtcttccat ccctgaattg atgcgttttg
      361 ttttgcttac gctggggatt ttaggggtga tgcttacgac aaatagcaaa ttggcgataa
      421 tctctctctt gcccatgatt ccgctaatcg taatgacagg ctggtttgga acaaagatcg
      481 ggaagctgtt ttttgaagtc gatcaggcgg taggagaggt ctctaatcgc ttgcaggaaa
      541 atgtgaccgg ggtacaggtc gtccgtgctt ttgcacgaga ggattatgaa atcaagcgtt
      601 ttgagtctgc caacatggat gtgtaccata aatggctgca tgtgattaat gagtgggcaa
      661 aaattatgcc aacgacaaac tggttgatca caattggaac gattttgatc ctgtacttcg
      721 gcgggcagat ggtgatttct ggcgaattaa ctgtcggtgc ggtggtggca ttcaacgcat
      781 atttacttat gatggcagaa ccagctcaag cgttgaccgg tttggttaac gcaggcggag
      841 aggcggcggc gggtgctcaa cgggtattcg aagttttgga tacagaacca gagatcaggt
      901 ccaagaaaga ggcgttggtt ttatccgaat tgaaaggcga ggtggaattt cataatgtct
      961 cgttgaaata tcaggatgag aatacgcgct cattggcgaa tatcaacgtg aaagtggaac
     1021 ggaataaaat agtggcgtta attggtccga ccggctctgg caagacatca cttgtaaatt
     1081 tgattccgcg tttttatgat gtgactgatg gtatggtgtt ggtggacgga caggatgtgc
     1141 gcgatgtaga tttgacttct cttcgcaggc agattgggat tgtgcttcaa acctctttgt
     1201 tgttttcaga cacgattcgc gcaaacattg cttacggaag accagatgca aagcaagaag
     1261 aaattgttgc ggcggcaaaa gctgctcagg cgcatgagtt tattgaaggc tttacgaagg
     1321 gatatgacac gattgttggc gaacgcggtg tgactctttc aggtgggcag cgtcagcgtg
     1381 tggcaattgc gcgtgcgttg ttgatgaacc cacgaatttt gatcttggat gattcgcttt
     1441 caagtgtgga tacacaaact gaaaaattga tccaagctgc attagatgta ttaatggagg
     1501 gtcgtacaac gttcgtgatt gcgcaccgtc tgagtacggt gcggcgggca gatatgattc
     1561 ttgtaatgga taaggggcgt atcgtggagc aaggcaccca cgatgagtta ataaaagcgg
     1621 gcgggcttta caaagaaatt cacgacttgc aattggtgga acgcgaagat gttattacgg
     1681 cagttcaacc tatttttggc gaaaagccat tgcatgagaa tactatctac tctggaaaat
     1741 caaattcggg ataatgccat gaccacaaaa ataattcctc ctgaatttat taccaaatct
     1801 gaagagcaac tcgataaagg ttacgaccct aaagttgcca gcgggttgat gaagtttgtc
     1861 aaaccgtatt atgggcagac gatttttgct ttgattttga tggtgatcgt taccgcggct
     1921 tcggtttcgg gcccatattt tgtgaagcgg gctattgatg aaggtattac agccaaggac
     1981 cctgttgcct tacgaaatat cacactcaca ttccttgttg tttcggttat acaactaggg
     2041 ctcaactatt ggcgcgtacg cattatggct cgtgtagggc agcatgtttt gtatgatgtg
     2101 cgcacagcca tgtttgccca tttgcagaag ctttcgttga gtttctataa tcgctatagc
     2161 gttgggcgtg ttatcacgcg tgtgatcaat gacgtgggaa ctttgcgtga gtttattact
     2221 tgggcggttc ttgcgatcgt acgtaatgta cttggcatcg ttggtacatt ggtggcaatg
     2281 atcgctttga attttcaact ttcattattg acctttatcg tcttgccgat catggtatgg
     2341 gcaacgattg cctttcgtaa agcggcgcgg caaaactatc gcaaggtgcg cgccgcggtt
     2401 tcgtggggca actcggtgtt ggcagaaaat gtcaacggag tgcgagtggt gcaagcattt
     2461 tcgcgccaac agcataacta caacaccttt agtgggtatg tcaatcgcta ttacctggaa
     2521 accagtctcg atgcggttcg cgtcgcttcg cttttcacac ccgtgatcga tttgcttggg
     2581 gcattggcaa ctgcattggt tatttatgtg ggcggaacgg cggtacttgg cgaatcgatt
     2641 acggctggcg tgttgattgc atttgttcta tatattgacc gcttctttga acctattcgt
     2701 gatttgagcc gccgctttga tacgcttcaa tccacgatgg caggtgggga acgcatactt
     2761 gagttactcc atacgccgat tgaggttcga gatgaagaac atgccaacga aatgggaccc
     2821 attaccggca cagtgcgatt cgataacgtc tcgttccatt attcggatga cccggttcta
     2881 gttcttgatt caattcaatt ggatgtaaag cctggtgaga cggttgccct tgtgggggag
     2941 accggtgctg gcaagacgac catcgttaag ttgttggcac gcttccacga cccgacctcg
     3001 ggcggggtgc tcattgacgg cgtggattta cggacggtga ctcaaggctc gcttcgcgct
     3061 caaatgggta tggtgttgca agatccgttc ctgttcaatg gtacggtgaa agaaaatatt
     3121 ttatttggtc gcttggacgc gaaagatgac gaggtaattg ctgctgcgaa agcggtgggc
     3181 gcccatgact tcatcatgaa tttgaaaaac gggtacgaca cgtcagttga agaaggcggt
     3241 ttgaccttga gtgtgggaca gcggcaatta atttcatttg cgcgtgcctt gttggcgaat
     3301 ccgcgtatcc ttattttgga cgaagcgact tcttctgttg acacgcagac cgagcatgtt
     3361 attcaaactg ccttagcaac tctcttgcaa ggacggactt cgttcgtgat tgcacatcgt
     3421 ttgtccacaa ttacaaatgc agataaaatt gtggtgatcg atggtggaaa aatcaccgaa
     3481 caagggaaac attcagaatt aattgcctta caaggcagat attacgaact ctataagacg
     3541 ggttttcagg agtaaaccat gacagaacca acgaacgaat ttctttataa aatgtccaag
     3601 catatgcgtg atgggcttgc cgatgtacaa aagaagatca atgacttgta caagattgaa
     3661 gagttggaag agtacgtgga tattccagat tctcccattg aacatctcaa ggcggcagta
     3721 atgcctctgg tgattcaggt caatgatttt atggattatg ccgagacggt cactgggcgc
     3781 atggagttgg acctacaaga tgtagacgtg catgcgttgc tcgaaggtgt gctgatgatg
     3841 gcagatcaat tagttgaaat gaaaggcggc attacgttgg aagaagatat tccagataat
     3901 cttccaacga ttgaaggtga ccctactcgg ctttcgcagg tgttattgaa ttacattcat
     3961 aatgctgtga agtttacaga gaaaggcaca gtgaccgtcc gcgtggcacg agagacagat
     4021 aaattacttt ttgaagtcag cgatacggga attggcattg ctgaagagaa tcaagagaaa
     4081 gtatttgaac ccttcgagac tattcttgaa gataaggatg atccacgcct tgggtttggg
     4141 atcggattga aaatcgttga acacattgtt gatctgcacg atggtgaaac ttggtttgaa
     4201 agtaaaaaag gtgcagggag ttccttcttt tttacagtgc cactataaat aaaaagagca
     4261 actccttgca gttgctcttt tgttatgaat tacttattca tcagtagaag acctaagtgc
     4321 tgaagttgat gctcaagttc tgaaacggaa ctccatggca agatttcaga atttttccgt
     4381 tcatctggcg ggttggatgg caaattgttg gaccagccgc catgccctac aagaggcaat
     4441 aagggacgtt tgtgtagcca agccaaagag acttcggata tggttccggc tcgcccgccg
     4501 atgaccaggc aggcgtcgcc ggcgagtgcc attatcaagt ttcgagcatc gccaatgcct
     4561 gatggtaaaa cgacagttcc gggccagtct gaagcgggca tttcatccga tggaataatg
     4621 ctaagcacca gaccgcctgc tttgatggcg ctttctgcag cgaagcgtgt ggctgggctg
     4681 ccacatccgc taacgagagt cacaccgtgg cgagcaagga aagcgccggc ttctccggca
     4741 aggtcatagg cagtggaacc tggctcggcg ctgcctaaca cgcaaacttg cggcttacga
     4801 atggaatggg gatgtatcat atctgatcct ttaaagtagt gtgtgattaa tgagatgtat
     4861 acctgtaatt gaggttttg
//
LOCUS       DAOQFT010000125         4875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9080_length_4875_cov_5.891909, whole genome shotgun sequence.
ACCESSION   DAOQFT010000125 DAOQFT010000000
VERSION     DAOQFT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9080_length_4875_cov_5.891909"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PLR93_04505"
     CDS             <1..1292
                     /locus_tag="PLR93_04505"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNH26255.1"
                     /translation="HPKSYELYQRARKSLHGGVPMLWMIRWAGSFPVFVKEAKGAHFT
                     DVDGNNYIDFCLGDTGAMTGHAPEATVKAITEQIQKGITLMLPYEDVIWVGEELQRRF
                     KLPYWQFALTATDANRFALRMARLITGRPKVLVFNYCYHGSVDETFITLDEEGTPISR
                     PNNMGPQIDPRETSKVIEFNDIAALETALSARDVAVVLAEPAMTNIGIIHPDPGYHEA
                     LRALTRKYGTYLIIDETHTICAGPGGYTASYGLEPDFITLGKPLAGGVPAAIYGFTEE
                     VSKAFSAKLAMDDADVGGIGGTLAGNALSIAAMKATLQNVLTDEFYAKATTLQEQFTA
                     GVEGVIEEFRLPWIVKRLGNRSEYWFRPTPPKNGGEAHAAVDPELDRYMHLYALNRGI
                     LMTPFHNMALISPETTQDDVDHHTKVFREAVKSLFGE"
     gene            1295..3025
                     /locus_tag="PLR93_04510"
     CDS             1295..3025
                     /locus_tag="PLR93_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNH26256.1"
                     /translation="MTDYKSQIWRINTRTQELKREPVPETWQRLGGRGLIARIMVDEV
                     DATCDPLGPGNKLIFTPGLLVGHILSSTDRISVGGKSPLTGGIKEANAGGRTGYHMAF
                     MGIHALILEDMPEKDGYWVLHLSLNDGAKWERADELVGLGVYETAPKLIEKYGDKVAI
                     SLIGPGGEMRMKSAGIQNLDKDKIPSRIAARGGLGAVMGSKGLKAIVFDHIGGQKPPI
                     ADVEAFKVAQKDYTQSVMNHPQSVTYREYGTQAMPQMTQNFAALPTHNFSRGTFDDVE
                     KISGETMREFILNRGKPSDPSHACMAGCTIKCSNVFGGEDGKIIVSPLEYETVGLMGT
                     NLDINSLDSIGRMNWQVNDLGLDTIEVGGALGVAAEAGIMRWGSEEDGLKLIDEIRKG
                     TELGRVLGDGAVAVGKKYGVERVPAVKGQSMSAYEPRSIKGTGVTYATTPQGADHTCG
                     LTIRAQVNHLDPTQQRDASLNAQLNMAGYDTIGACIFAGFGYAATPDGVVKRLLKARY
                     GWEDVPDNVLQALGKETIKLEREFNKRAGFTKEDDRLPKWMMEEALPENGSVFDVSED
                     VLDHIFDGIE"
     gene            complement(3094..4671)
                     /locus_tag="PLR93_04515"
     CDS             complement(3094..4671)
                     /locus_tag="PLR93_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH26257.1"
                     /translation="MEREKLAFFNPATNTKFGEVVVNTPEEVQQAVREMRQAFPEWSR
                     KSIQERADILHKLQHVLIEQRDEISNVLNMDCGKSRQDGLLELFVAIDMLVQYRHNAK
                     KWLRRQRVSSGLYIFKRCYIEHRPHGVTAIISPWNYPFTLSLPPMVSALLAGNTVVLK
                     ASEVTAATGVLIEKLIKSVPELAPFVRVVHGDGRVGAALVASKPDYIFMTGSTATGKK
                     IMQTAAETLTPVALELGGKDAMIVLEDAELEAAARWGAWGAFFNTGQTCMSVERVYVV
                     ESKYDEFVNLAVLQAQELKSGYSTELNSPFFMGPVTDQRQVKIIDGHLQDALDKGAKI
                     LTGGKRDGMFYDPIVLVNVDHSMKIMRDETFGPVMPIVKVKNEEEAIHLANDNAYGLG
                     ASIWSNDISHAKRVADRVEAGSIIINDSIAQFGVPMLPFGGTKDSGFGRTHGKEGLLQ
                     FTRPYSFAIGGAPVKMDVATVLREMGHYELATMIMGVLFGTTLKQKVETFLQFFRRDK
                     KQAPPAGETSSNTAAAD"
BASE COUNT         1221 a         1130 c         1243 g         1281 t
ORIGIN      
        1 cccaccccaa atcgtatgag ctgtatcaac gggcgcgcaa atctctgcat ggtggtgtgc
       61 ccatgttgtg gatgattcgc tgggcagggt cttttccggt gtttgtcaaa gaagccaaag
      121 gggcacattt tacagacgtg gatggcaaca actatatcga cttttgtctc ggcgatacgg
      181 gcgccatgac aggtcacgct cccgaagcga cggttaaagc cattactgag caaattcaaa
      241 agggcattac cctaatgttg ccctatgaag atgtcatttg ggtaggtgag gaacttcaac
      301 gccgcttcaa gttgccgtat tggcaatttg ctcttactgc tacagatgcg aatcgctttg
      361 cgcttcgcat ggcacgcctt atcacagggc gccccaaggt tttagttttc aactattgct
      421 atcacggttc tgtcgatgaa accttcatca cgcttgatga agaaggaacg cccatctctc
      481 gcccaaacaa tatgggtcca cagatcgacc cgcgcgaaac tagcaaggtc attgaattca
      541 atgatattgc cgccctcgaa acggcgctct ccgctcgcga tgtggcagtt gtacttgccg
      601 aacctgccat gacgaatatt ggcatcatcc accctgaccc cggctatcat gaagctttgc
      661 gtgcattgac gcgcaaatac gggacctatc tcatcattga cgagacgcac accatttgtg
      721 ctggtcccgg cggatacacc gcttcttacg gtcttgagcc tgatttcatc accctcggca
      781 aacccctcgc tggaggcgtt cccgccgcga tctatggttt tacagaggaa gtctcgaaag
      841 cattttctgc taaacttgcc atggacgatg ccgatgtcgg tggtattggt ggcacattgg
      901 caggtaatgc tctgtccatt gcggcgatga aggcaacact tcagaatgtg ctcactgatg
      961 agttctatgc caaagcgacg acgttacagg aacagtttac agcgggagtc gaaggcgtga
     1021 ttgaagaatt caggcttccg tggattgtga agcgtttagg aaatcgctct gaatactggt
     1081 tccgtcccac acctccaaag aacggcggcg aagctcatgc tgccgtagat ccagaattgg
     1141 atcgttacat gcacttgtat gcgctaaatc gcggtatatt gatgacgccc tttcataaca
     1201 tggcgctcat ctcacctgaa accactcaag atgatgtgga tcatcataca aaagttttta
     1261 gagaagctgt gaagagtttg ttcggagagt gataatgact gactataaat ctcaaatctg
     1321 gcgtattaat actcgtactc aagaattgaa gcgtgaacct gtccctgaaa cctggcaacg
     1381 cctcggcggg cgcggactca ttgcacgcat catggtggat gaagtggatg caacgtgtga
     1441 cccacttgga cccggcaata agttgatttt tacacccggc ttgttagtag ggcatatcct
     1501 ttcctccaca gaccgtattt cggtaggcgg taagtctccg ctgacgggcg gaatcaagga
     1561 agccaatgcg ggcgggcgca cgggctatca catggcgttt atgggtatcc atgcactcat
     1621 tcttgaagat atgccggaaa aagatggcta ctgggttttg cacttgtctt tgaacgatgg
     1681 cgcaaaatgg gagcgagccg atgagcttgt tggcttgggt gtgtatgaaa ctgctccgaa
     1741 actgatcgaa aaatatggcg ataaagttgc catttcactc atcggtcctg gtggcgagat
     1801 gcgtatgaag tcagcaggga tacagaatct agacaaagat aaaatccctt cgcgtattgc
     1861 tgcgcgtggc gggttaggtg cggtgatggg ctcgaagggt ttgaaagcta ttgtgtttga
     1921 ccacataggc ggacaaaaac ctccaattgc tgatgtagaa gcattcaagg ttgctcagaa
     1981 agactacacc caatcggtca tgaaccatcc gcagtctgtg acataccgcg agtatggtac
     2041 gcaagccatg ccgcagatga cccagaattt tgcagcgttg ccaactcata atttctcgcg
     2101 tggcactttt gacgatgtag aaaagattag cggcgaaacc atgcgcgagt ttattttgaa
     2161 tcgtggcaaa ccctccgacc cctctcatgc ttgtatggcg gggtgtacga tcaagtgctc
     2221 aaacgtgttt ggcggtgaag atggcaagat cattgtttcg ccgttggaat atgagacggt
     2281 tggtttgatg ggcaccaacc ttgatatcaa ttcgctcgac tccattggac gcatgaattg
     2341 gcaagttaat gaccttggtt tggataccat cgaagtaggt ggagcattag gggtcgctgc
     2401 cgaagcggga attatgcgct ggggcagtga agaagacggc ttgaaattga ttgatgaaat
     2461 tcgaaaaggc acagaacttg gacgtgtcct tggcgacggg gcggtagcag ttggaaagaa
     2521 atatggagtt gaacgtgttc ctgcggtcaa aggtcagtct atgtccgcat atgaaccgcg
     2581 ttccattaaa gggaccggtg tgacttatgc gaccactccg caaggtgcag accatacttg
     2641 tgggttaacg atccgtgctc aggttaatca ccttgacccc actcagcaaa gggatgcgtc
     2701 tctcaacgcg cagttgaaca tggcgggcta cgataccatc ggtgcatgta tttttgcagg
     2761 cttcggctat gccgcaaccc ctgatggggt agtgaaacgt ctattgaagg cgcgctatgg
     2821 ttgggaagat gtgcccgata acgtgttgca agctcttggc aaggagacta ttaaactaga
     2881 gcgcgagttc aacaaacgcg cagggtttac caaagaagat gaccgtttac ccaaatggat
     2941 gatggaagaa gctttgccag aaaatggttc tgtctttgat gtgagcgaag atgtgcttga
     3001 tcatattttt gatggaattg aatagaagaa cataattggt ctgcaagatt gagaataaaa
     3061 actgccctgt aggaattcta cagggcagtt tgattaatca gccgcagctg tattggaact
     3121 ggtctcgccg gctggcgggg cttgtttttt gtccctgcgg aagaattgta aaaaggtctc
     3181 tactttttgt ttgagggtgg tgccaaatag aactcccata atcatggttg ctaactcata
     3241 gtgtcccatt tcgcgcaaaa cggttgcaac atccattttg acgggcgcac cgccaatagc
     3301 aaagctatat gggcgagtaa attgtaataa gccttctttg ccgtgtgtgc gaccaaaacc
     3361 tgagtccttt gtgccgccaa aaggcagcat gggaacacca aattgagcga tactatcatt
     3421 gatgatgatc gatcccgcct caactcgatc cgcaactcgt ttggcatggc tgatatcgtt
     3481 gctccaaatg gaagcgccta aaccataagc attgtcattt gcgaggtgta tggcttcttc
     3541 ttcgttcttc actttaacta tgggcataac cggaccaaat gtttcatcgc gcatgatctt
     3601 catgctgtgg tctacattga caagcacaat cgggtcatag aacatcccat cacgtttgcc
     3661 gcctgtaaga attttggcgc ctttatccaa cgcatcttgc aaatgtccat caatgatttt
     3721 aacttggcgc tggtcagtga cgggtcccat aaagaatggg ctattgagtt cggtggaata
     3781 accgctcttt agctcttgtg cctgcagcac tgccagattt acaaactcgt catattttga
     3841 ctcgaccaca tagacacgtt ctacagacat gcacgtctga ccggtattga agaaggctcc
     3901 ccaggctccc caacgagcag cggcttctag ctctgcatct tcaagcacga tcatagcgtc
     3961 tttcccgcca agttccaatg ctactggggt aagtgtctct gccgcagttt gcataatctt
     4021 cttgccggtg gctgtggagc cagtcataaa aatgtagtct ggcttgctgg caaccaaggc
     4081 tgcacccact ctgccgtcac catgaacaac acgtacaaat ggcgctaact ctggtacgct
     4141 cttaatgagc ttttcgatca atacgcctgt agcagcagtc acttcagagg cttttagaac
     4201 tacagtattc ccagcaagta aggctgagac catgggtggt agagataatg taaatgggta
     4261 gttccatggt gagataatgg cggtcacacc gtgggggcga tgttcaatgt aacagcgttt
     4321 gaaaatgtaa agcccagagg aaacccgttg acggcggagc cattttttag cattgtgtcg
     4381 atattggaca agcatgtcta tggcaacgaa taactcaagc aacccgtcct gacggctttt
     4441 gccacagtcc atgttgagca cattgctgat ttcgtcacgt tgctcgatta gcacatgttg
     4501 caacttgtga agaatgtccg cccgttcttg gatgctttta cggctccatt ctgggaatgc
     4561 ctgccgcatt tcgcgaacgg cttgttgcac ttcttcagga gtgtttacga caacctcacc
     4621 aaactttgtg ttggtggcag gattgaaaaa tgctagcttt tcgcgttcca tgaaaggttc
     4681 cttggcattt gaaattgttt ttcttttcca attataacca gttgtttatg gaaattggct
     4741 ccaaaaagtg agaaaagagt ctgttttctc ctctttatta tgcctgagtt tgcaatcttt
     4801 ttttcatcag tagccagcct gtgacgccga ataagagcga aacaaagagc ccttcatttg
     4861 taggcgtaaa gaccg
//
LOCUS       DAOQFT010000126         4839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9179_length_4839_cov_6.673495, whole genome shotgun sequence.
ACCESSION   DAOQFT010000126 DAOQFT010000000
VERSION     DAOQFT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9179_length_4839_cov_6.673495"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="PLR93_04520"
     CDS             complement(<1..2261)
                     /locus_tag="PLR93_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNH26258.1"
                     /translation="MTEIFISYCRRNKGFTEQFIQGLAEQNYPADKIWIDWNNIPPSS
                     KWEDEIRKGIEACNAVVFILSPDWILSNECRKELEIAIEYNKRLFPIIHQNIDPQSSP
                     AALASLNWIFFREADDFKGAMQKLMAAINTDLDWVKQHTVLLKRANEWNSKKQDGSYL
                     LRGSELQGAELWLSKSSDNKQPHPTPLQSEYIFASRQDASRRQRNTLIGVSAALVVSI
                     LLAIAAIVGGVEAVRKSQQALASQLAAQSITLVDTQPDLSLLLSLESNYIGDTLGDAD
                     PASLGSLVSSINSVPQLDTYLRAHAGEIREVTFSSNGRWVAATGNAINDQGVVTVWDL
                     ASKENPIADQQFIGGTQRILGLAFSPDSKRIAAAGDEKKIFIWDPQTCCETVKEFEID
                     NKARSLKFVTLNGKEYIAIASGQQITFWDSSTGKKDEALTLQISTTDETVRILSLAVA
                     PTNDMVAAGSEDGNITVWELKTKEVKFQACSFGDPETNNEEICKVNGDGETDIRGLAF
                     NANGTLLASGSSDHRVWLWNPETGDLLARSAVSTEGGHINTVTSVTFNSKTGTIASVS
                     WDNTVRIWEPIQNEDKTWGLELVESLTGHSNSIWAAAYSPDGIHLATGSSDKTLILWN
                     VRQASQLGVPFKKLEGDAWALAVSPDKKHFAAGDTFGGIHVWDFDGNTLSNEQKIPNE
                     GGVLALAYSNDSKWLASSGYSDNTIRVWDAATGKELWSLENAHDQEIWALAFSPDNRW
                     LASASFDQTVKIW"
     gene            2464..3405
                     /gene="fabD"
                     /locus_tag="PLR93_04525"
     CDS             2464..3405
                     /gene="fabD"
                     /locus_tag="PLR93_04525"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNH26259.1"
                     /translation="MKLNLNNTAFVFPGQGSQTVGMGKSLAEEQTIAKEVFAEADSIL
                     GFALSDLMWNGPDVELNDTVNTQPALFIHSIASFRTFTRLYPDLVPAALAGHSLGELS
                     ALTASGALSFEDGLRLVRKRGELMKRAGELAPGSMAAILGVDIPTLDKVCAEASVTDE
                     LVQVANDNCPGQVVISGSKAAVERAMAGAKAAGAKRALPLAVSIAAHSPLMASIQDEW
                     NQAVDEASMHHPKTPVIGNVHASVLKTAEEARADIKAQMQSRVKWTDSVKTISNMGVT
                     AFVEVGTGSVLGGLVKRIVSDAVSYPLGNPQDFSALE"
     gene            3489..4715
                     /locus_tag="PLR93_04530"
     CDS             3489..4715
                     /locus_tag="PLR93_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNH26260.1"
                     /translation="MNKKLAALLIFIFLTACAPLDIETPYTPVTQIPFYASDTPAPFI
                     VPVTETPQIIFPVTETPTLEQPVFVTDTPFPVAKWWEVYFTEPLTINDLNNPAGSIEE
                     KLINFINAAQVSIHIASFEFNLPRVADALIAAKARGVDVRWVTDDENGLEIDGNANRG
                     QFTRLMAAGVEVKDDAGRTALMHNKFWLFDRQITWTGSTNITVNGIYKQNNNVIVIHS
                     PEVTEIYEREWEELWTAQLGPRAPSTKNNQWAILDGTPLQILFSAEDDAVDSLIAILN
                     DAQVSIRFLAFSFTDYPMAQAMIARAQAGVDVQGIFETFGSNSTRAELKTLWCAGLPV
                     RQDGNSSFLHDKVIIVDNSIVVTGSLNFSSSADEENEENVVILDNPEIAALYLQEYQK
                     LWDQAMSVPTGAFTCE"
BASE COUNT         1223 a         1096 c         1146 g         1374 t
ORIGIN      
        1 tcccatatct ttacggtttg atcaaagctg gcagaggcaa gccaacgatt atctgggctg
       61 aaggcgagcg cccaaatttc ctgatcatgg gcattttcaa ggctccataa ttctttgcct
      121 gttgcggcat cccaaacgcg gatggtgttg tctgagtagc ctgacgatgc aagccatttg
      181 ctatcgttac tgtaagctag tgctaatacg ccaccttcgt ttgggatttt ttgctcattg
      241 cttaaggtgt ttccatcaaa atcccataca tggatgccgc cgaatgtatc tccagcagcg
      301 aaatgttttt tatctggcga gactgccaat gcccaggcat caccttccaa ttttttgaat
      361 ggaaccccga gttgacttgc ctgtcttaca ttccaaagga ttagggtttt atctgaactt
      421 ccggtggcaa gatgaatgcc atctgggctg tatgctgctg cccaaatcga attggaatga
      481 cctgttagag attctacaag ttcaagcccc caagttttat cttcattctg aattggttcc
      541 caaatacgaa cagtattgtc ccaacttaca gaagcaatgg ttcctgtttt cgagttgaag
      601 gttactgagg taaccgtgtt gatgtgacca ccttcggtgc tgaccgcaga gcgagctaaa
      661 aggtcaccgg tttctggatt ccagagccag acacgatggt cggagcttcc tgatgcaagt
      721 aaggttccat ttgcattgaa cgccaacccg cggatatccg tttcaccgtc tccgtttact
      781 ttacaaattt cctcgttgtt ggtttcaggg tcgccgaaac tacaggcttg gaatttaact
      841 tcttttgtct ttagctccca aactgtgata ttcccgtctt cacttccagc tgctaccata
      901 tcgtttgttg gagcaacggc aaggctgaga atacgaacgg tttcatcggt agtagagatt
      961 tgtagagtaa gggcttcatc ttttttacct gtggatgagt cccagaatgt gatttgttga
     1021 ccagaagcaa tggcgatgta ttcttttccg tttagggtaa caaactttaa agaacgtgct
     1081 ttgttgtcaa tttcaaattc ttttacggtt tcacagcaag tttgtggatc ccagataaag
     1141 atttttttct catctccagc cgctgcaatg cgtttgctgt ctgggctgaa agcaagacct
     1201 aaaatacgtt gtgtgccacc aataaattgt tgatctgcga tcgggttttc ttttgaagcc
     1261 agatcccaaa cagtgacaac gccttggtcg ttgatcgcat ttcccgttgc agcaacccat
     1321 cttccattcg aactgaacgt cacttcacga atctcacctg catgtgcccg caaataagta
     1381 tccaactgtg ggacgctgtt gatgctggaa accaaacttc ctaaacttgc cgggtctgca
     1441 tcgccaaggg tgtcaccaat gtaatttgac tcaaggctca gaagtaaaga aaggtcaggt
     1501 tgtgtgtcta ccaaagtgat ggattgagcg gcaagctgac tcgccagtgc ttgctgactt
     1561 ttacgcaccg cctccactcc gccgacgatg gcggcaattg ccaataaaat agaaacgacc
     1621 aatgctgcgc tgacgccaat caaggtattg cgttggcggc ggcttgcatc ttggcggctg
     1681 gcaaagatgt attcactttg aagtggcgtt ggatgtggtt gtttattgtc agaactttta
     1741 gaaagccata actctgcccc ttgtaattca ctaccgcgta acagataact gccatcttgt
     1801 ttttttgaat tccattcgtt ggctcgcttc aaaagaacag tatgttgttt gacccaatcc
     1861 agatcagtat tgatcgctgc cattaatttt tgcatggcac ccttgaagtc atcggcttcg
     1921 cggaagaaaa tccaatttaa ggatgccaat gcggcaggag aactttgcgg gtcaatattt
     1981 tggtgaatga tggggaagag ccgcttgttg tattcaatcg caatctctaa ctctttgcgg
     2041 cactcgttgg aaagaatcca atcagggcta agaatgaaga ccacagcatt acatgcttca
     2101 atgccttttc gaatctcatc ctcccattta gaggaaggtg gaatattgtt ccaatcgatc
     2161 cagattttgt ctgcggggta attctgttct gcaagcccct gtataaattg ctcggtaaat
     2221 cctttgttgc ggcgacaata tgagatgaaa atttctgtca tgatctggct ccttgattca
     2281 catgatctga aaagcacttt ttgcccatca aacctgcttt tggcggcata gaaaacttgc
     2341 aatgattttg caattttact cctgcccatg cgtgaccata tttgcaattt tgtgtttaat
     2401 agccatgcct aacaatgctg taaatagctg aaatatattg atttttagcc acaaggagac
     2461 ggaatgaaat taaatttgaa caacacagcc tttgtcttcc ccggacaagg ctctcaaacg
     2521 gtagggatgg gaaaatccct tgccgaagaa caaacgatag ccaaagaagt ctttgccgaa
     2581 gcggattcga tcctcggctt tgcactctca gacctgatgt ggaatggacc agacgttgaa
     2641 ttaaatgata cggtcaacac gcaacctgca ttatttattc attctattgc ttcttttcgg
     2701 acattcaccc gcctctaccc ggaccttgta cctgctgcac ttgccgggca ttcacttggc
     2761 gagttatcgg ctctgactgc atctggagcc ttatcttttg aagacgggtt acgcctcgtg
     2821 cgcaaacgcg gtgagttgat gaagcgtgca ggtgaactgg ctcccggtag catggctgcc
     2881 atcctcggtg tagacattcc cacacttgat aaagtttgtg ccgaagccag tgtcacagat
     2941 gagttggtac aagttgcaaa tgataattgc cctgggcaag tggtgatctc tggctccaaa
     3001 gcagcagtgg agcgcgccat ggcaggtgcg aaagctgctg gtgcaaaacg tgccttgcca
     3061 ttggcagtgt cgattgcggc tcactccccg ttgatggcgt ctattcaaga tgaatggaat
     3121 caggctgtag atgaagccag tatgcaccac ccgaagacgc cagtgattgg gaacgtccac
     3181 gcttctgtgc ttaaaacagc agaagaagct cgagccgata tcaaagcgca gatgcaatct
     3241 cgcgtgaaat ggacggatag tgtaaaaacc atcagtaaca tgggcgtgac tgcttttgtt
     3301 gaggtaggga caggctctgt attgggtggt ttggtcaagc gtattgtgag tgatgcagtc
     3361 agctatccgt tagggaatcc gcaagatttt tcagcattag aataagtttt ttacggttaa
     3421 gtcactcaga gtgacagtcg ctttccggta caatacagcg actgtcactt ttgattcagg
     3481 aacccataat gaacaagaaa ctagctgctc tcctgatttt tatcttcctt accgcttgtg
     3541 ctcctctcga tattgaaacc ccatatacac cggtcacaca gattcctttt tatgccagtg
     3601 atacgcctgc gccttttatt gttccggtaa ctgagactcc gcagatcatt tttcccgtca
     3661 cagagacgcc tactcttgaa caacctgtct ttgtaaccga tactcctttc cccgttgcaa
     3721 aatggtggga agtgtatttc accgaaccat tgacgattaa tgacctcaat aaccccgccg
     3781 ggtctataga agaaaaattg attaacttta tcaatgccgc tcaagttagc attcacattg
     3841 cttcgtttga attcaacttg ccacgtgtag cggatgcttt gatcgccgcc aaagctcgcg
     3901 gtgtagacgt gcgttgggtg acagacgatg aaaacgggtt ggaaatagat ggcaatgcga
     3961 atcgggggca gttcactcgt ttgatggctg ccggagtcga agtcaaggat gatgcggggc
     4021 gtactgcctt gatgcataac aaattctggc tgttcgaccg tcaaatcaca tggacaggct
     4081 ccacgaatat caccgtcaat ggaatttaca agcaaaacaa taatgtgatt gtgatccact
     4141 cgcccgaagt gacggagatt tatgagcgcg aatgggaaga attgtggacg gcacaactgg
     4201 gtccgcgggc accttctacg aaaaataatc agtgggcgat cttagatggc actccattac
     4261 agattctttt ctctgctgaa gatgacgctg tggatagcct gattgccatt ttgaacgatg
     4321 cccaagtgag cattcgtttt cttgccttta gctttaccga ttaccccatg gcacaagcca
     4381 tgatcgcacg cgcccaagcc ggggtagatg tgcagggaat ctttgagacc tttggcagta
     4441 atagcactcg tgccgagtta aagacgcttt ggtgtgcagg cttgccggtg cggcaggatg
     4501 gtaatagcag tttcttgcat gacaaagtta ttattgttga taacagtatt gtggtgaccg
     4561 gttcgctaaa tttttcatca agcgcagatg aagagaatga agagaatgtt gtgattttag
     4621 ataaccctga aattgccgcc ttgtatttgc aggaatatca aaaactatgg gatcaagcaa
     4681 tgagtgtacc gacaggcgct tttacttgcg aataaaggat atagggtaaa agttaaataa
     4741 aaaacgcctc ggaaatttac ttccgaggcg ttttttatta tttgtacatg gttgctgcta
     4801 caggcttggc aagcgcgccg ctcttggcat tgcgcatgg
//
LOCUS       DAOQFT010000127         4813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9260_length_4813_cov_5.014922, whole genome shotgun sequence.
ACCESSION   DAOQFT010000127 DAOQFT010000000
VERSION     DAOQFT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9260_length_4813_cov_5.014922"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLR93_04535"
     CDS             <1..406
                     /locus_tag="PLR93_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNH26261.1"
                     /translation="SIFRSADGFGFDHVYLGGITPTPENEAVIKTSLGAEEYLPWSYH
                     KDAVKLIHGLKKDGWWIAALEEGEGSIQLGKQKSTKAKKSVLILGSEVTGVDPELLAL
                     CDQIISIPMRGQKRSLNVAIAFSVAAYALSNA"
     gene            418..1083
                     /locus_tag="PLR93_04540"
     CDS             418..1083
                     /locus_tag="PLR93_04540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH26262.1"
                     /translation="MFSKKSIFMMIAVATLVFSSFACKALAGTPQTSAEPAQGNTSTC
                     SLFDSIPAAGQYSSAPDMLINANGNYTATLTLAKGEEIVIELYASEAPLTVNNFIFLA
                     CKGFYNNTTFHRVLQDFMAQGGDPTGTGMGGPGYEFVNEYSDLTFDKPGVVAMANAGP
                     DTNGSQFFITFGPTEWLNGGYTIFGQVVQGMDAVMSITLRDPDANPSFTGDVLQSVTI
                     SGQ"
     gene            complement(1118..1996)
                     /locus_tag="PLR93_04545"
     CDS             complement(1118..1996)
                     /locus_tag="PLR93_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH26263.1"
                     /translation="MKRSFARFTLLFFFLTTACYQELQTDASLPVVTSVATSGFTPLP
                     PTITVTPTSIFSPAQALAAGPINIIAIGDDFTQGEGDTLGRGYTGLLIELASQIRPGS
                     TIVNFGRTGWDSNDLLNGKDEFSGQLDLAVSEVKSAASQRRAAVVLVWVGGNDLWELY
                     SGTTEVTIEMEEQDALRFAQNMDTIVFELRNAKAEVIIAKLDDQSQRPAKTRGEVYPD
                     ITNDELLRMSMQVQRYNESISAIATKYNALTVDFFNSEIFTQSTTLAADGYHPNPTGY
                     ELISQAWYKALIKILP"
     gene            complement(2016..2663)
                     /locus_tag="PLR93_04550"
     CDS             complement(2016..2663)
                     /locus_tag="PLR93_04550"
                     /inference="COORDINATES: protein motif:HMM:NF022152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HNH26264.1"
                     /translation="MDSSFIYELIGYAASLLVAFSLTMKSLQKLRIVNMIGAVVFIVY
                     GLLIGAIPVAFLNTLILGVNVYNLWQMWQQKDYFTLMQVRADSAYLKKFLEFYRTEIS
                     EFVPTYQFKPADDQIVVFILRNMVPVGVVIVKPEGEDARIFLDFVIPGYRDFRAGKFL
                     FEESTEFYRQLGIQRLSTAPGSKRHETYLHRMGFKLEEDGRYYYELPIRIIREHI"
     gene            2808..3002
                     /locus_tag="PLR93_04555"
     CDS             2808..3002
                     /locus_tag="PLR93_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNH26265.1"
                     /translation="MNVQRQALRRSRHDRIIAGVCGGLGEFFGISPFWFRLAMFIAFI
                     PGGVPGILIYFIAMLIIPSE"
     gene            complement(3057..3620)
                     /locus_tag="PLR93_04560"
     CDS             complement(3057..3620)
                     /locus_tag="PLR93_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH26266.1"
                     /translation="MQYKKLLQIFVLLAVVVSAFGFTSGASAWYCTSYVTVQWGDTLS
                     GIASLCGTTVDAIRAANPGLGWWVYAGQVLYIPTGSTPSYPAPSYGGTYVVQWGDTMA
                     KIAGYYGVTVNDLLAANPQVWSPSLIYAGQVLNIPAAPAYHTVSYGETLRMIAGYYGT
                     TVYSLQLLNPTIYDPNYIYPGQSIRVR"
     gene            complement(3784..4092)
                     /locus_tag="PLR93_04565"
     CDS             complement(3784..4092)
                     /locus_tag="PLR93_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26267.1"
                     /translation="MTNQWYSLGLGDGMLAAAPSEEIQVYFAKVFSAMGMPPEMAVFT
                     RHESEGRLHCEVVAYFSPKASEVAQAFDAEPCPKPVRAGLGLLAGSETAWSNLFDEAQ
                     "
     gene            4266..4727
                     /locus_tag="PLR93_04570"
     CDS             4266..4727
                     /locus_tag="PLR93_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26268.1"
                     /translation="MPFAYRLDKNIKIFFLVTYGHCTFSDIFSPSLDPETKPENRPRV
                     KFILDNMLGDLEIDMEGMKFFIKAMNELKSTGFQLEPTAFITNKRGLEIFIKSLDLLV
                     DNDVTTHMACSSLGEALIWLDEVENAQAIQRIRDELLETLRLQYGDHPQLV"
BASE COUNT         1363 a         1134 c         1085 g         1231 t
ORIGIN      
        1 ctcgattttt cgcagcgcag atgggttcgg gttcgaccat gtgtatctgg gtggaatcac
       61 acccacacca gaaaatgaag cggtcataaa aacgtctttg ggtgcggaag agtatcttcc
      121 ctggtcgtat cacaaggatg cagtcaaatt aatccatggg ttgaaaaaag acggctggtg
      181 gattgctgcg cttgaagaag gcgaaggatc tattcagctt ggaaaacaaa agagcacaaa
      241 agctaaaaag tctgtattga tccttgggag tgaagtgacc ggggtagacc ccgaattact
      301 tgcgctctgt gaccaaatca tatccatccc catgcgcgga caaaaacgct cgctaaatgt
      361 tgccattgct tttagcgtag cagcctacgc attatccaac gcctaaagga gaatttcatg
      421 ttttcaaaaa aatcaatttt tatgatgatt gccgttgcaa ctctggtctt ttccagtttt
      481 gcttgcaagg cattagcagg cacaccccaa acttcagctg aacctgcaca gggaaacacc
      541 tccacgtgca gcttgtttga tagtattcca gcagcagggc agtattcatc agcaccagac
      601 atgcttatca atgccaatgg gaactataca gccactctca ccctcgcaaa gggcgaagaa
      661 attgtgattg agctttatgc cagcgaagcg ccgctcactg taaacaattt tatctttctt
      721 gcctgcaaag gtttttacaa caacacgacc ttccaccgcg tcttgcaaga tttcatggca
      781 caaggcggtg acccaactgg caccggcatg ggtggacccg ggtatgaatt tgtgaatgaa
      841 tacagtgatt tgacctttga taaacccggt gtggttgcca tggcgaatgc aggaccggat
      901 acaaatggca gtcagttttt tattacgttt ggtcctacag aatggctaaa tggcgggtac
      961 acaatctttg ggcaagttgt tcaaggtatg gatgcagtca tgtccatcac attgcgtgac
     1021 ccagatgcaa acccatcctt cactggagat gttcttcaaa gcgtaactat ctcaggacaa
     1081 taatcaaaac ggccgcccaa ttggcggctg ttttctttta gggcaaaatc tttatgagcg
     1141 ccttatacca agcttgagag atcaattcat aacccgtagg gttaggatga tacccatctg
     1201 cggcgagcgt tgtagattgg gtaaaaatct cgctattgaa aaaatctacg gtaagagcat
     1261 tgtattttgt ggcgatagca gagatacttt cgttataacg ctgcacttgc atggacatgc
     1321 gaagtaattc atcatttgta atatctggat acacttcacc gcgggtcttt gccggacgtt
     1381 gtgattggtc atccagcttg gcaatgatga cctccgcctt cgcatttcgc aactcaaaga
     1441 cgattgtgtc catattttgg gcgaaacgga gcgcatcttg ttcttccatt tcaatggtga
     1501 cttcagttgt acctgaatac aattcccaaa ggtcattacc gcccacccaa actagaacta
     1561 cagcggctct acgctgtgaa gccgcagact tgacctcact cacagctaaa tctaactgcc
     1621 cagaaaattc atctttgccg ttgagcaggt cattcgagtc ccaaccggtg cgcccaaagt
     1681 tgacaattgt agagcccggg cgtatttggc tggctaactc tattaatagt ccggtgtatc
     1741 cgcgcccgag cgtatcccct tcgccttggg tgaagtcatc gccgatggca atgatgttga
     1801 tcggaccagc agccaaggct tgagcgggag aaaaaatcga agtgggcgtc accgttatgg
     1861 tcggaggaag aggcgtaaat ccactggttg caacggaagt cactacgggg agtgatgcat
     1921 ctgtttgaag ctcttgatag caggctgttg tcaaaaagaa aaaaagcagg gtaaaccgcg
     1981 caaacgaacg cttcattgat tgcctcgatg aaagatcaaa tatgttctcg aataatgcgg
     2041 atgggaagtt cgtagtaata ccggccgtct tcttccagtt taaaccccat gcggtgcagg
     2101 tatgtttcat gccgtttgct gcccggcgcc gtggacaggc gctgaattcc caattgacga
     2161 taaaactcgg tgctttcttc aaataggaat ttgcctgcgc gaaaatcgcg atagccggga
     2221 atgacaaaat ctaaaaagat gcgagcatct tcgccttcag gcttcacaat cacaacaccc
     2281 acaggcacca tattgcgcaa aataaaaacc acaatttggt catcggcagg tttgaattga
     2341 taggtaggaa caaattcaga aatctcagtg cggtagaact caaggaactt tttcaggtaa
     2401 gcactatctg cgcggacttg catgagtgtg aaataatctt tttgctgcca catctgccac
     2461 aggttataga cattaacccc caagattaag gtattgagaa atgccactgg aatggctccg
     2521 atcaacaagc catacacaat gaaaacaact gcgccgatca tgttcacaat acgcagcttt
     2581 tgcagagatt tcattgtcag tgaaaatgcc accaacaaag atgcggcata cccgatgagt
     2641 tcgtaaataa aagatgagtc catttttctt cctgcgttga attttgaagc ggggcattat
     2701 acactggata attttttcaa tttcaagcca gtctggacaa ctactgtaat tttatctgaa
     2761 accataggga aaattgtgcg tatatctcta cataaaaagg agaaaaaatg aacgtacaaa
     2821 gacaggcttt aagacgctca agacatgacc gcatcatcgc cggggtatgt ggcgggcttg
     2881 gcgaattctt tggcattagc cccttctggt ttcgcctcgc catgtttatc gcttttattc
     2941 caggtggcgt gcccgggata ttgatctact ttattgcgat gcttatcatc ccatctgaat
     3001 aatgtagttt aaacaaaaag aagtccgcac catagcggac ttctttttgt ttgtttttat
     3061 cgtactcgaa tggactgacc ggggtaaata taattcgggt cgtagatcgt ggggttcagc
     3121 aattgcaggc tgtaaaccgt tgtgccgtaa tatccggcaa tcatacgcaa tgtctcacca
     3181 taggagacag tatggtaagc aggtgccgcc gggatgttaa gcacttgccc agcatagatg
     3241 aggcttgggc tccaaacttg cgggttggca gccagcaagt cgttgacggt cacgccataa
     3301 taaccagcaa tctttgccat agtgtcaccc cattgcacta cataggttcc accatacgaa
     3361 ggcgcagggt acgatggtgt agacccggtt gggatgtaga ggacctgccc ggcgtacacc
     3421 caccagccca aaccagggtt ggcagcgcga attgcatcca ccgtggtgcc gcagagagag
     3481 gcaattccgc tcagggtgtc accccactgc acagtcacat acgaagtgca ataccaagca
     3541 gaggcgccgc tggtaaagcc gaaggcagaa acaacaactg ccagcaaaac aaatatttgt
     3601 aaaagtttct tatattgcat atcatcctcc attgatggtt gaaaacaaaa acgactcttc
     3661 ttaaattata tagattccac gcgcgaattc aatcacacga atgtacttat gaatctaata
     3721 acgctaaaga gtcctctttt gttcctctag agattccctc tgcaatgaaa ttagaatgga
     3781 gttttattgc gcttcgtcaa acaaattgga ccaagccgtc tcactgcctg ccaagagtcc
     3841 cagcccggct cttactggtt ttgggcaggg ttcggcatcg aatgcctgag ccacctctga
     3901 ggcttttggc gaaaaataag ccaccacttc acaatgcaac cgtccttcag attcgtggcg
     3961 agtaaaaacc gccatttcag gcggcattcc catcgccgaa aaaactttcg caaaataaac
     4021 ttgaatttct tctgatggag cggctgccaa catgccgtcg cctaacccaa gggaatacca
     4081 ttgatttgtc atattggagt tttccatttt gaatactggc acaaataatt cagactaaaa
     4141 atgtcgcatt gatatgtaag tctaacactc tatactagaa tattacaaaa taaattgtca
     4201 tcagcgcctg ttatacttga aacaagttat caaccaacac cccaaccacg aagtggacat
     4261 ttgttatgcc ctttgcatat cgccttgata aaaatatcaa aattttcttc cttgtcacct
     4321 atggtcactg tacattcagc gacatcttca gcccatcact agatccagaa accaaacccg
     4381 aaaatcgtcc cagagtcaaa ttcattctcg ataatatgct aggcgacctt gaaatagaca
     4441 tggaaggcat gaaatttttc atcaaagcga tgaatgagtt gaaaagcaca ggctttcaac
     4501 tggaacccac tgcgttcata accaataaaa gagggcttga aattttcatt aaatcacttg
     4561 atctgcttgt agataatgat gtgacaaccc atatggcatg ctcttcgctt ggtgaagcgc
     4621 taatctggct ggacgaagtt gaaaatgccc aagccattca acgcatccga gatgagttgc
     4681 ttgaaacgtt acgtttgcaa tatggtgatc atccgcaatt ggtgtaaaag ctaaactgcc
     4741 cccgcaggga ctcgactcgc gccacccgcg cccgcaaaca caaccgtagg ggcgactcgc
     4801 cagggactga ctc
//
LOCUS       DAOQFT010000128         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9564_length_4710_cov_5.906982, whole genome shotgun sequence.
ACCESSION   DAOQFT010000128 DAOQFT010000000
VERSION     DAOQFT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9564_length_4710_cov_5.906982"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLR93_04575"
     CDS             complement(<1..424)
                     /locus_tag="PLR93_04575"
                     /inference="COORDINATES: protein motif:HMM:NF027503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNH26269.1"
                     /translation="MAKKKEMEGEVYYPSEQVVAQARLKDWDALAEKAEKDLEGFWAA
                     EASELEWYKKWDKVLDDSKAPFYKWFVGARTNIVHNAVDRHLKTHRKNQLALIWESED
                     GKSERTFSYFAMNREVSRMANIIKAMGIVKGDRVTIYMG"
     gene            complement(505..2442)
                     /locus_tag="PLR93_04580"
     CDS             complement(505..2442)
                     /locus_tag="PLR93_04580"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNH26270.1"
                     /translation="MLSLRLNGDTDMNLISPIVNRWIQDGKEDPDGFWEKAAKELYWF
                     RVWDRVFEWNYPNFRWFVGAQTNLSYNALDVHVKQGWGGHTALIYMNERDERRLFTYA
                     KLLHDVEKIAAALRGMGISKGDRITIYMPTCPEAIMLMLATVRIGAIHSVVFAGFGEK
                     ALGDRIQASGSRLVFTTDITYRKGKDVNLKSIVDGALAQVDNSVEHLVVLKRGSQDVP
                     MNTPLDITWEEFLEHSKGQPSNCVPMEANEPAFILATSGTTAKPKLAIHTHGGYQVHI
                     ASMARWVFGLKPEDVWWATSDIGWIVGHSYIVYAPLIIGCTTIAFEGALDHPSSETNW
                     KIAIEEFSVSGVFTSPTAVRLLMRYGDEPLGRVNHDRVERIFCAGEVLNAPAWDWLQN
                     KMLKNRIPVIDHMWQTETGGPVFGNPYGLGMLPIKPGSATIPLPGIQVEVMNMDGQPC
                     APNEKGIMVIKRPFPGLTPALWGEPERYGNDYWNKIPNVYYTGDSAHMDADGYVWFAG
                     RADEIIKIAGHRIGTIEVETAFLKHPAVAESGVIGRPDEIRGEVISAFVLLKNGFQAS
                     EALKKELLETVRHELGPVAVIGELNFVNMLPKTRSGKIMRRVLKAVTLDKDPGDITTI
                     EDEGSVTEAREAWQEMKNALS"
     gene            complement(2475..3896)
                     /locus_tag="PLR93_04585"
     CDS             complement(2475..3896)
                     /locus_tag="PLR93_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26271.1"
                     /translation="MSQQSVTASADQEEAQSLPRIIFASAAGTMIEWYDFYIFGSLAT
                     VIASKFYQTGTDIGDIIAWLATFAIGFIVRPFGAIVFGRVGDLIGRKYTFLVTMTLMG
                     LCTFAVGLLPTIEVLGSLAGVLLITLRILQGLALGGEYGGAATYVAEHSPHGRRGFYT
                     SWIQITATLGLFMSLGVILATRKIVGEDAFKEFGWRIPFLISILLVAVSLWIRYSLRE
                     SPMYTKLKKSGGISKNPLKESFGNPYNLGFVGLALFGATMGQGVVWYTGQFYALFYLQ
                     KIFGVDLVDSNMIIGGALLFATPFFVFFGNLSDKIGRKNIMLTGMVLAVVTWLPIYQT
                     MAKYSPFIDFSSAADAVNTPNPAYSPFMLGVLIFIQVLYVTMVYGPIAAFLVELFPTK
                     IRYTSMSLPYHIGNGVFGGLVPIIGLELITKTGNNFAGLWYPTAIAAICAVICYFKIP
                     ETYKVDIESDASVAAIGAKVSGD"
     gene            complement(4331..>4710)
                     /locus_tag="PLR93_04590"
     CDS             complement(4331..>4710)
                     /locus_tag="PLR93_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH26272.1"
                     /translation="HAKSLAYSEGAELILLTVAANPAMDYLFSDPGIAETAVQEQVEK
                     SKAYISTIEKELSTAGFKVSTLMRVGSVAEVILSVADELQVDVIAMSTHGRTGAARWL
                     LGSVAERVVHNSNVPVLLIRVKE"
BASE COUNT         1250 a         1175 c         1084 g         1201 t
ORIGIN      
        1 gacccatata aatagtgaca cggtcgcctt tgacaatgcc catggctttg atgatgtttg
       61 ccatgcgtga aacttcgcgg ttcatggcga agtatgagaa ggtgcgttca gacttcccgt
      121 cttccgattc ccaaatcaga gccagttggt tcttgcgatg ggttttaaga tgtcggtcta
      181 cagcattgtg gacgatgttt gtgcgcgcac cgacaaacca tttgtagaac ggcgcttttg
      241 aatcatccaa aactttgtcc cactttttat accattccaa ttcagaggct tctgccgccc
      301 aaaagccttc gaggtctttt tcagcttttt ctgccagcgc gtcccaatct ttcaaacgtg
      361 cctgtgccac cacttgctca gatggataat agacttcgcc ttccatttcc ttcttcttcg
      421 ccatgccagc ctccaaaagt gatatgtgga tgttagcggg tattttaatc cgcttttcta
      481 aaattgcgta agagtgttac tcatttaaga tagggcgttt ttcatttctt gccaggcttc
      541 acgagcttca gtgacggagc cttcatcttc aatcgtggtg atgtcaccgg ggtctttatc
      601 gagggtgact gcttttagta cacgcctcat gatcttgccg gagcgggttt tgggcaacat
      661 attgacgaag tttaactcgc cgataactgc cacaggtcca agctcgtggc ggacggtttc
      721 aagcaattct tttttcagcg cctcagaagc ttggaaccca ttcttgagca atacaaaggc
      781 tgagatgacc tctccacgaa tctcatcggg tctgccgatg acgcccgact ctgctacggc
      841 tgggtgcttg agaaatgccg tttctacctc gatggtgcca atgcgatgtc cagcgatctt
      901 gatgatttca tctgcccgcc ctgcaaacca cacgtaacca tctgcatcca tatgagccga
      961 gtcgccagtg tagtagacgt tgggaatctt gttccaataa tcgtttccat atcgttctgg
     1021 ctcgccccat aaagcaggag tcaaccctgg gaaggggcgt ttgatgacca taatgccttt
     1081 ttcgtttgga gcgcatggct gaccgtccat attcattacc tcaacctgaa tgccgggtag
     1141 gggaatggtc gccgagccgg gtttgatggg caacatccct aagccgtagg ggttcccaaa
     1201 aacagggccg cccgtttcgg tttgccacat atggtcaata actgggatgc gattcttcaa
     1261 catcttgttc tgcaaccaat cccaggcagg tgcgtttaga acttcgcctg cacaaaagat
     1321 gcgttctacg cgatcgtgat taacacgacc tagcggttcg tctccatatc gcatcaataa
     1381 acggacagca gttggagatg tgaaaacccc gctaactgaa aattcttcaa tggcgatctt
     1441 ccaattcgtt tcagaacttg gatgatcgag cgccccttca aaggcgatgg tcgtacagcc
     1501 gatgatgagc ggcgcataga caatataact gtgtcccacg atccaaccga tatcagaggt
     1561 tgcccaccat acatcttcgg gctttaatcc aaagacccaa cgtgccatac ttgcaatgtg
     1621 gacttgatag cctccatgag tatgaatggc taattttggt ttggcagtgg taccagaagt
     1681 agcaaggatg aaagctggtt cattagcttc catcggaaca caattgctag gttgaccctt
     1741 ggagtgttca aggaattctt cccaagtaat atcgagcgga gtattcatag gtacatcttg
     1801 agaaccgcgt ttcaagacca caagatgttc cacgctgtta tctacttgtg ccaaggcgcc
     1861 gtcgacgata ctttttagat taacatcttt ccccttgcgg tatgtgatat ctgtcgtgaa
     1921 aaccaatctc gagccgctgg cttggatgcg gtcacctaac gctttttcgc cgaaacccgc
     1981 aaacactacc gagtgaattg caccaatgcg gacggttgct agcatcagca taatggcttc
     2041 agggcaggtt ggcatataaa tagtaatgcg gtcgcctttg gaaatgccca ttccgcgtaa
     2101 ggcggcagcg attttttcaa cgtcgtggag cagttttgca tacgtaaaga ggcgacgttc
     2161 atcacgctca ttcatataaa taagcgcggt atgacctccc cagccttgtt tgacatgcac
     2221 atccaacgcg ttataagaaa gatttgtttg cgcccctacg aaccaacgaa aatttggata
     2281 attccattca aaaacacgat cccacactcg gaaccaatat aactcttttg ctgccttttc
     2341 ccaaaatcca tctgggtctt ctttaccatc ttgtatccaa cgattaacga ttgggctgat
     2401 cagattcatg tctgtatctc cattgaggcg gagcgacaac atcgctccgc ctcaagttgt
     2461 ccgtttgtat ttgtttaatc cccagagact tttgcgccaa tggctgctac agaagcatct
     2521 gattcaatgt caactttgta ggtttcagga attttgaagt agcagatcac agcacagatc
     2581 gccgcaatgg ctgttggata ccaaagccca gcgaagttat taccggtctt tgtgatcaat
     2641 tccaagccga tgatcggaac caagccgcca aagacgccat tgccgatgtg atacggaagc
     2701 gacatggagg tgtagcgaat tttggtaggg aagagttcca ctaagaaagc cgcaatcggt
     2761 ccatatacca tggtcacata gagcacttgg atgaagatca acacaccaag catgaaggga
     2821 ctatatgccg ggttgggcgt attgacagca tctgccgccg aactgaaatc aatgaacggg
     2881 gagtatttcg ccatggtctg gtaaatcggc agccaggtga caacagccaa gaccatgcct
     2941 gtgagcatga tattttttcg accgatcttg tcggataaat tgccaaagaa gacaaaaaat
     3001 ggagtggcga aaagcagcgc tccgccgata atcatattcg aatcgaccag atccacaccg
     3061 aatatttttt gcaggtagaa cagcgcatag aattgcccgg tataccagac aacgccttgt
     3121 cccattgtgg cgccaaagag ggccaacccc acaaatccta aattgtaagg gttaccaaag
     3181 ctttccttaa gcgggttctt ggaaatgccg cccgatttct tgagcttggt gtacatcgga
     3241 gattcgcgta gtgagtagcg gatccaaagt gaaacagcca ccaaaaggat tgaaataagg
     3301 aatgggatgc gccagccaaa ttctttgaag gcatcttcgc ccacgatctt gcgcgtggcg
     3361 aggatcacac caagagacat gaacaacccc aatgtcgcag tgatttgaat ccagcttgtg
     3421 tagaagccgc gcctgccatg cggtgaatgt tcagctacat aagttgctgc gccgccatat
     3481 tcgccgccca acgccagccc ttgcaaaatg cgtaatgtga tcaaaagaac accagccaat
     3541 gaaccaagaa cttcaatggt gggcaataaa ccgaccgcaa atgtacacag acccatcaag
     3601 gtcatggtaa caagaaaggt atatttccgc ccaataagat caccaacacg tccaaataca
     3661 atcgcgccaa acgggcgcac aataaaacca atcgcaaaag ttgccagcca ggcaataata
     3721 tcgccaatgt cagtaccggt ctggtagaac ttactagcga tcacggttgc cagactgcca
     3781 aaaatataaa aatcgtacca ttcgatcatg gtaccggcag cagatgcaaa aataatacgg
     3841 ggtagacttt gcgcctcttc ttggtcagcg gaggctgtta cagactgttg agacataatg
     3901 cagttctcct ttgggaagat aaatggatat gttccgcgca tcgcggttgt aacaggctgg
     3961 gtgatcacta ggcagaataa gtcatcagcg ctcctttact ccgagaacat ccattacccc
     4021 atggacgagc agaagaagcg catatcaaac cgcgtattgg ggaaaagatt ctttcgggca
     4081 ttggtacctc gaacaaaaac cttgatccac ctaaccacta aagggtttgc atatttcaga
     4141 aaataacact aagtttgccg aattcgttat gttgatttca acgccaaatg tcatggattt
     4201 tttcgataat tgtcatgtgg taacgtgaaa ataaatttca gacaaaaatt gtccgaaaga
     4261 aaaacggctc tttccccgct cttgccgggt acaacactca aaagactcgt ccccctgcag
     4321 gtttttacac ttactctttc acgcggatca atagaactgg tacgtttgaa ttatgcacaa
     4381 cccgttctgc cacacttcca agcagccagc gtgccgcacc cgtccgccca tgtgtggaca
     4441 tggcgatgac atccacttgt agttcatctg ccacgctgag gattacttct gccaccgagc
     4501 cgacccgcat caaggtagaa accttgaacc cggcagtgct aagctctttt tcaatggtag
     4561 aaatatatgc cttacttttt tcaacttgct cctgaaccgc ggtttccgca atgccagggt
     4621 ctgaaaaaag atagtccatg gctgggttgg ctgcaacggt cagcagaatt aactcggcgc
     4681 cttcagaata tgccaatgat tttgcgtggg
//
LOCUS       DAOQFT010000129         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9711_length_4662_cov_5.848926, whole genome shotgun sequence.
ACCESSION   DAOQFT010000129 DAOQFT010000000
VERSION     DAOQFT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9711_length_4662_cov_5.848926"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLR93_04595"
     CDS             complement(<1..520)
                     /locus_tag="PLR93_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26273.1"
                     /translation="MKTKNIFLTFVIVCTGLAACAPPPLSSTSNGSAPSTDSQPITDS
                     PESVAPTESETHEAADANHSHTSDVDLTHLELGDGKYSTSPQMGYVYTCQTQFNGGGA
                     TGTGSWMNGDGTWDATKKAVVDGSITWPHSFTITIQGDQRVFVGNNLPNHPTGNYPIL
                     PSDDAYAVDRNPN"
     gene            complement(673..1437)
                     /locus_tag="PLR93_04600"
     CDS             complement(673..1437)
                     /locus_tag="PLR93_04600"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNH26274.1"
                     /translation="MLLTIDIGNTNLTLGLYEGDTLGAHWRLSTDHNRMPDEYGLQFL
                     GVLQNAGKTIKDIQGISVASVVPPLTGRVIQACREYLKQEPLVVDAGVKTGIKIRYED
                     PKAVGADRVCDAVAVMKLYGGPACVVDFGTATTFNAVTRDGEYLGGAITAGINLAAEA
                     LYTRAAKLPRIDLQVPPSVIGRNTVHAMQSGLLFGYVSMVEGMVNRFRAELGSDMKVV
                     ATGGLAEVVAHETKVIDIIAPWLTLEGLRLIWEMNR"
     gene            complement(1441..2796)
                     /locus_tag="PLR93_04605"
     CDS             complement(1441..2796)
                     /locus_tag="PLR93_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26275.1"
                     /translation="MTSLLSFIVLLPRIFLGFFIVHSIWNVKDGKSLLVKIFLSAGVG
                     FGVSSLFSFLWIWVGLPLTVYAWVESILAILLMIWLLFQNREILRIPQVAVKQEFLWL
                     SFLAAGALFFALNLILFGLQYPHGRPDAWINWNVVARFIYLGGADWQATFLRQWDHPD
                     YPFLVPMTNAITWVFLGNASTWGPVALHFVLSLFAAGLLFAFVNIFRGFKQSVLSVLI
                     FTSLPFVVNQGMRQYSDFLVAYLILAAGGLTLMYLQTKEKRIAVLVGLVIGLAGWAKN
                     EGLMAILSVSFVWVLLALKKEGRTGLLRYVIGLVFPVVVIVLFKIFLAPSNDLMSAQE
                     SLADKIFDIERYKTIFSIAIPMLWNLGNTPISIIGLIILTALLVRRSTNRITGLWALA
                     VIVLIQLTAYFGIFVLTPRDLAWHLNTSLDRLYLHILPLAFLLFFLWLKSPQELLSKE
                     S"
     gene            complement(2797..3222)
                     /locus_tag="PLR93_04610"
     CDS             complement(2797..3222)
                     /locus_tag="PLR93_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26276.1"
                     /translation="MTLLRKLIPFVLVAMSLAMAIYQFVLSFSAGEWRNSSEEMVSKW
                     EDRIQSLREALPADVKQVGYIDDAVISEDPALFDANEFQLMQYSVAPVAIDSEKRHEW
                     MIGNFNDDKNLEAWLTEQLGAYELQGFGFGLYLIHDVEN"
     gene            complement(3241..>4662)
                     /locus_tag="PLR93_04615"
     CDS             complement(3241..>4662)
                     /locus_tag="PLR93_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26277.1"
                     /translation="QHPSMFTIEYIRVPQTWMPLFLLTALASAILSLFLITRYLSHIT
                     HYSQFTIHHLPGLYATGMFFLIELIISRTINHPALEKNTVLFEADAGPWMTILASPES
                     APINRAVHPLSLILIRPLVRLVSGFMGEHYALGGMIVVSLVAAACVFMTWLFVKRATN
                     SSTYAFIFAIFLGSTATHLLFGSLAENYIFGAAALIFFFLLIQSNETRFSVLVPAGLF
                     LFGITITNIAQGAIALFFNKFGFKKLIQYSLLILSLGILLTVTTNIIYPKHITYFFVP
                     EDIAFEFNFVSPENAGVLSDPIRLSEPSSITRKLNVVSRSILLYGIVAPDVIESISEK
                     PPFPTIDLKTFDVRTKAIASYRGLSNLPLVLWLILLAGAFFMFVKTWRTSKYAPLMLG
                     LLGALGFNFLMHLFYGTELFLYTSYWVYAFVLFIALALSDLAEKTWLEWGLGIILLTL
                     MVNNFHFIFSIFQSLAPFYAAAP"
BASE COUNT         1400 a         1038 c         1201 g         1023 t
ORIGIN      
        1 aattcgggtt gcgatctaca gcataggcat catcgctggg taaaattggg taattcccag
       61 tgggatggtt gggaagatta ttcccaacga acacacgctg atctccctgt atggtgatcg
      121 tgaaagaatg cggccaggtg atggaaccat cgacaactgc cttcttggtc gcatcccagg
      181 tgccatctcc gttcatccag gaccctgtgc ccgtcgcgcc gccgccattg aattgagttt
      241 gacaggtgta cacatatccc atttgcgggc tggtggaata tttaccgtca ccaagttcaa
      301 ggtgagttaa gtccacatcg gaagtatgag agtgatttgc atctgctgct tcatgggttt
      361 cgctctcggt tggagcaaca gattcgggcg aatctgttat gggctgagag tctgttgatg
      421 gggcagaccc attcgaggtc gaagaaagag gcggaggcgc gcacgccgcc agtccagtac
      481 atacgatcac aaatgttaaa aatatatttt tcgttttcac gtcattctcc tctaatgaat
      541 tgattagcaa tagtttatag ccagtgggtt gacatgcaga gtgtgtgaaa tcacagcttt
      601 tttacaataa gcatgagatg aatcacaacg acccatgacg gctgtcatgg gtcgttggca
      661 ggtgcaagat ttctagcggt tcatttccca gatcaggcgc agaccctcga gggtgagcca
      721 gggggcgatg atatcgatga ctttggtttc gtgtgcgacg acttctgcca gcccgccggt
      781 tgcgacgact ttcatatcgc ttcccaactc cgcccggaat cggttcacca ttccctcaac
      841 catggagacg tagccgaaga ggagtccgga ttgcatggca tggacggtat tgcgcccaat
      901 gacagaaggc ggcacttgca aatcaatgcg tgggagtttg gctgcacgcg tgtaaagcgc
      961 ttcggcagcg agattgatcc ccgctgtgat ggcgccgcca agatattcac catctctggt
     1021 aacggcattg aaagtagtcg cggtgccgaa gtccaccaca caagcgggac cgccgtaaag
     1081 tttcatcaca gccactgcgt cacaaacgcg gtccgcaccg acagctttgg ggtcttcata
     1141 gcggatcttg atgccggtct tcacacccgc atccaccacc agcggctctt gtttaagata
     1201 ttcgcggcag gcttgaatca cacgcccggt caacggcggg acgaccgatg cgactgaaat
     1261 gccttgaata tctttgatgg tctttccggc attttgcaat acgcctaaaa actgcaagcc
     1321 atattcatct ggcatacggt tgtggtctgt agagagacgc caatgcgcgc caagcgtatc
     1381 accttcgtat aaaccaagcg taaggttggt attgccaatg tcgatcgtaa gaagcatggg
     1441 tcaactctct tttgataaaa gttcttgcgg agatttcagc cagaggaaga acaaaagaaa
     1501 tgcaagtgga aggatgtgta aatacaaacg atctaaagaa gtattcaggt gccaagccag
     1561 atcacgagga gtaaggacaa aaattccgaa ataggctgtc aactgaataa gaacgataac
     1621 agcaagcgcc catagacctg taatacgatt tgttgatcta cgaacaagaa gtgcggtcaa
     1681 aattataagt ccaattatgc tgataggcgt attccccaga ttccacagca tggggattgc
     1741 aatactaaag atggttttgt aacgctcgat atcgaaaatc ttatccgcca agctttcttg
     1801 agcagacatc aagtcattgg aaggggcaag gaaaatctta aagagcacaa tcactacaac
     1861 agggaaaacc aaaccgataa catatcttaa taatccagtt cgtccctctt ttttcaaagc
     1921 caataaaacc catacaaaag acacagacag gatcgccatt agtccttcgt tctttgccca
     1981 gcctgccaac ccaatcacaa gaccaacaag gacggcaatc cgtttctctt ttgtttgcag
     2041 gtacattaag gtcaagccgc ctgccgctaa gatgagataa gcgaccagaa aatctgaata
     2101 ctgacgcatt ccctgattaa caacaaatgg aagcgaagtg aatataagaa ccgataaaac
     2161 ggattgttta aagccgcgaa aaatgtttac aaatgcaaac aacaatcctg ctgcaaagag
     2221 agacaatacg aagtgtaaag caactggtcc ccaagtggat gcgttgccta aaaacaccca
     2281 agtaatggcg ttcgtcattg gcaccaaaaa cggataatcg gggtgatccc attggcgaag
     2341 aaatgtcgct tgccaatccg caccgccgag gtagatgaaa cgcgcgacca cgttccagtt
     2401 gatccacgca tcggggcgcc catgtgggta ttgcaatccg aaaagaatca gattcaacgc
     2461 aaagaaaaga gcaccagcag caagaaatga aagccaaaga aattcttgtt tcacagcgac
     2521 ctgtggtata cgcaaaattt cgcggttttg gaagaggagc caaatcatca gaaggattgc
     2581 aagaatgctt tcgacccaag catagaccgt taatggaagt ccgacccata tccataaaaa
     2641 gctaaagaga gatgaaacgc caaaccccac cccggcagac agaaagattt taaccagcag
     2701 gcttttgcca tctttcacat tccaaatgga atggacgatg aaaaagccta aaaagattct
     2761 tggcagcaaa acaatgaatg acagaagcga cgtcatctaa ttttccacat cgtgaataag
     2821 atacaagcca aacccaaagc cttgaagttc atatgcccct aattgctccg tcagccatgc
     2881 ttcaaggttt ttatcatcgt tgaaattacc gatcatccac tcgtgacgct tctctgaatc
     2941 aattgcaacc ggtgccaccg aatattgcat caattggaat tcattggcat caaacaacgc
     3001 gggatcttcg ctgatcacgg catcgtcaat gtacccaact tgcttcacat ccgcaggcaa
     3061 cgcctcacgc agggactgaa tccgatcctc ccatttggag accatctcct ctgatgaatt
     3121 tctccactca cccgcggaaa agctcaaaac aaattgataa attgccattg caagcgacat
     3181 ggcaaccaaa acaaatggga tcaactttcg caggagggtc atgcaggtat tttaaccggt
     3241 ctacggggca gccgcgtaaa agggagcaag agattgaaag atcgagaaaa tgaagtggaa
     3301 attattgacc atcaaggtca agagaataat tccaagcccc cactcgagcc aagtcttttc
     3361 tgcgaggtca gacaatgcca aagcaataaa taagacaaaa gcgtagaccc aatatgaggt
     3421 gtaaaggaat aactctgtgc cataaaacag gtgcatgaga aagttaaatc ccaaagcacc
     3481 gagcaagccg agcatcaatg gagcgtattt ggaagtccgc caggttttta cgaacatgaa
     3541 aaatgcgcct gccaacaaga tcaaccacag aacaagtggt aagttggaaa gtccgcggta
     3601 ggaggcgatg gctttagtac ggacatcaaa ggttttcagg tcaatggttg ggaagggcgg
     3661 tttttcggaa atggattcga tgacatcggg tgcgacaatt ccgtagagca agatggagcg
     3721 cgaaacgacg ttcaacttgc gggtaatgga cgaaggttct gataaacgga tagggtctga
     3781 aagaacgccc gcattttcag ggctgacaaa gttgaattca aaagcaatat cttcaggcac
     3841 gaaaaaatag gtgatgtgtt tgggatagat gatattggta gtaacagtca gcaaaatgcc
     3901 gaggcttagg atgagcaagg aatattgaat cagctttttg aaaccgaact tattgaaaaa
     3961 gagggcgatg gctccctgag caatgttggt tatggtaatg ccaaaaagaa agagcccggc
     4021 agggacgaga accgaaaaac gagtttcgtt agattggatt agtaaaaaga aaaagataag
     4081 agctgctgcg ccgaagatgt agttctctgc taatgagcca aagagcagat gcgtggcggt
     4141 cgagccgagg aaaatagcga agataaaagc atacgtgctg gagttcgtgg cgcgtttgac
     4201 gaagagccag gtcatgaaga cacaggcggc agcaaccaat gaaacgacga tcatgccgcc
     4261 gagggcgtag tgttcaccca tgaagccgga gaccagtcgc accagtgggc gaatgagaat
     4321 caggcttaac ggatgcacgg cacggttgat cggggcactc tccgggctgg caaggatggt
     4381 catccacgga ccagcgtcgg cttcgaagag gacggtgttc ttctccagcg cgggatggtt
     4441 gatagtgcgg gagatgatga gttcgatgag gaagaacatt ccggtagcgt agagaccggg
     4501 aagatggtga atagtgaatt gtgaatagtg agtaatgtgg gataagtaac gagtgatgag
     4561 gaataaggag aggatagcgg aggcgagggc ggtgaggagg aagaggggca tccatgtttg
     4621 ggggacgcgg atgtactcga tggtgaacat ggaggggtgt tg
//
LOCUS       DAOQFT010000130         4645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9754_length_4645_cov_7.076471, whole genome shotgun sequence.
ACCESSION   DAOQFT010000130 DAOQFT010000000
VERSION     DAOQFT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9754_length_4645_cov_7.076471"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..1082
                     /locus_tag="PLR93_04620"
     CDS             429..1082
                     /locus_tag="PLR93_04620"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNH26278.1"
                     /translation="MAKKKYSINWEDDLPVSFEVNGVQYQNIEDVPDEKDRLKLEAML
                     NSSFDAEINDPQLEKMMKETQQMDGASAEAWIVRIFTNVAVVMLLVAGVSTVFVLQKL
                     GREESAPGVVVDVVEQRYEDAETDNVSYYYYPVVEFKARDGKRRTVQLTEGSNPQSNE
                     VGDEVTILYEPENPNDARIKSFGSSALMWILPSITGILGAGFLGAVLVFQKYLANVS"
     gene            complement(1114..1692)
                     /locus_tag="PLR93_04625"
     CDS             complement(1114..1692)
                     /locus_tag="PLR93_04625"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HNH26279.1"
                     /translation="MSIFEIVYWAALVVEMVIRAPLNKKRKEEKISEQRVSQQEKLIL
                     FLLLIGMMILPLIYSATSWLDFANYNLPPWAGYLGALIMLSALFVFWRSHADLGLNWS
                     PSLEIRDKHELITRGIYGVIRHPMYASQWLWVLAQPLLLQNWLAGFINLLVFIPFYLL
                     RVQAEEKMMIDRFGEQYKTYMTKTGRVVPKLG"
     assembly_gap    1832..1931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2069..2647)
                     /locus_tag="PLR93_04630"
     CDS             complement(2069..2647)
                     /locus_tag="PLR93_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26280.1"
                     /translation="MPRKPALNTDEILNSEYQYIAQNVFQSNEDRSRVTSFYFVSVGS
                     FVAAILGTRFEPDQSTISLAFFFLFTILTFMGALTIAQLARLRAAWHEAVEAMNQIKD
                     YYIKHMPELEPAFKWRKRQIPPTNKPYSIANLMAVEVALLSALTTGAGIYFLMTYIGI
                     SNWVTWSAVIVSVIFGYIAQWTWYRYLLVDNQ"
     gene            complement(2749..3984)
                     /locus_tag="PLR93_04635"
     CDS             complement(2749..3984)
                     /locus_tag="PLR93_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNH26281.1"
                     /translation="MNETFQTSLEKYADIIVNIGLNLHKEQRLNIAAGLPDAELVRLV
                     AKKAYQLGSPFVDVRWIDERLTRIRFENSKPEHLVEVPQWQIDREHEYGSRGDALLSF
                     ASQDPNLLEGISPDLIGRYRKAISEKYEPVTKYRNENTQTWCVVSTATPAWAQKVFPD
                     LPVEQAQEKLWEAIFDACRVNTADPVQAWREHAQKLHRQCDYLNEKRYAGLHYKAPGT
                     DLTLGLPDNHLWIGASGKTQNGIEYIPNLPTEEVFSMPHREKTEGYVTSSRPLILSGT
                     MIDEFTLTFENGRVNKVTAKQGEENIHKLLETDENASRFGEAALVPDSSPISQRKHLF
                     YNTLFDENASSHLALGTAYRYNVKGGTSMTKEEFIAAGGNDSLIHVDFMIGSDQMDID
                     GIRADGTREAVMRKGEWAF"
     gene            complement(4105..>4645)
                     /locus_tag="PLR93_04640"
     CDS             complement(4105..>4645)
                     /locus_tag="PLR93_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNH26282.1"
                     /translation="SMAQNGVAFTANMPTEEVFTLPDRNRADGVISATFPLSYGGTLI
                     ENFQVHFENGRITKVTAKKGEAALQKLVDTDEGSHRLGEIALVPASSPIGKRGHLFYN
                     TLYDENASCHIAIGRAYRFTLAGGTELNDDEFTAAGGNISLNHVDFMIGSPQMDIDGI
                     TKDGKHEPVMRQGEWAISL"
BASE COUNT         1140 a          969 c         1195 g         1241 t
ORIGIN      
        1 cggcggcgac ggggtcggtc acgccggggc ggagggaaac ttccagaata accgaatcag
       61 gttcgggcgg agtgagaggc gcgggcaact catcccatga aacgttttgt gtcacagggt
      121 cgcttaaaag tttgagagcc aactgttgca atccttcctg cgacaattgg ctctcgatga
      181 agtagaggtc ctgacattcc accctttgaa gctggtggaa tccaagcgcg tgtgcaccgc
      241 gccggtaact ttcattccgt gggtcgttgt ttttttgttg aacagaaaat ttatagatgc
      301 tcatttttac ctgtagaata ggcgctattg tatccatgta aatttgtcta gtcaatgcat
      361 gaatttgtta gcatgtcaga catttcgatg tgatttcacc tgatagaatt ttgcgctcgg
      421 aggtatgaat ggcaaagaaa aaatattcca tcaattggga agatgatctt cctgtttcgt
      481 ttgaagtgaa tggtgtgcaa tatcaaaaca ttgaagatgt gcccgatgaa aaggaccgcc
      541 tcaagttgga agcgatgttg aattcttctt ttgacgcaga gatcaatgac cctcaacttg
      601 aaaagatgat gaaggaaact cagcagatgg atggtgcctc tgctgaagcg tggatcgtgc
      661 gtatctttac gaatgttgcc gttgtgatgt tattagtggc tggggtttct actgtatttg
      721 tcttgcaaaa actggggcgt gaagagagtg cgcccggtgt tgttgtggat gtggttgagc
      781 agcgttatga agatgctgag acggacaacg tcagctacta ctattatccg gtagtggagt
      841 tcaaggcacg agatggaaag cgccgcacag ttcaattgac ggagggtagt aacccgcaat
      901 cgaatgaggt gggggatgag gtgacgattt tgtatgagcc agaaaacccg aatgacgccc
      961 gcattaagtc ttttggtagc tctgccttga tgtggatttt gccaagcatt acgggtattt
     1021 taggcgctgg gttcttgggt gcggtacttg tatttcagaa gtatttagca aacgtttcgt
     1081 gaaaaaaatc ccgccatctg gcgggatttt tctctaacca agttttggca ctactctgcc
     1141 tgttttggtc atatacgttt tgtattgttc gccgaagcgg tctatcatca ttttttcttc
     1201 tgcttgaaca cgcaagagat agaagggaat gaacaccaga aggttgataa acccagccag
     1261 ccaattttgc agcagtaaag gttgtgcaag cacccataac cattgcgaag catacatggg
     1321 gtgacggatc acgccataaa tgccacgggt aatgagctca tgcttatcgc gaatttccaa
     1381 ggatggagac cagttcaagc cgaggtctgc gtgagagcgc cagaaaacga ataaagcgct
     1441 tagcataatc aatgcaccca aataaccagc ccaaggcggc aagttatagt ttgcaaaatc
     1501 cagccatgaa gtagcggagt agataagagg gagaatcatc ataccaatga gaagcaagaa
     1561 gaggataagc ttttcttgtt gcgaaacgcg ttgttcgctt attttttctt ctttgcgttt
     1621 cttattaagc ggggcgcgaa tgaccatctc cacaaccaaa gccgcccaat aaacgatttc
     1681 gaaaatgctc atagggtctc tgttttttca acaattacag ttgcttctgt gagcaaggct
     1741 gcaatagcgc ccaatgcggc aagctgtggg gcgattaaca caccgacaac accgatcgtg
     1801 acggggattt ccattaagac cttgccatct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn ntccactgaa gtggcaaagt gacccacttt gaaagtattg atattttcgc
     1981 gaatccacgc atcgtctttg gtgaggtgcg gtctgcgctg aaaagcagtg ggattctgac
     2041 cgatgtattc tcttggcatg aggttctcct attgattgtc tacgagtaga tagcgatacc
     2101 aggtccactg ggcgatgtag ccaaagataa cgctgacaat gactgctgac catgtgaccc
     2161 aatttgaaat gccgatatag gtcatgagga aatagatgcc agcgccggta gtgagcgcgc
     2221 tgagcaaagc cacttccact gccatgagat tggcaatgct atatggcttg ttggtgggtg
     2281 gaatctgtcg ctttcgccat ttgaaggcgg gctccaactc gggcatatgt ttgatgtaat
     2341 aatccttaat ttgattcatc gcttctactg cttcatgcca cgcggcgcgc agacgggcga
     2401 gctgggcaat ggtcaacgca cccatgaaag ttagaatggt gaagaggaag aaaaaagcta
     2461 aagagatggt gctttgatct ggctcgaagc gtgtgcccag aatggcggcg acaaaagagc
     2521 caaccgaaac gaagtaaaaa gaggtcaccc gcgagcggtc ttcattcgat tgaaaaacgt
     2581 tctgggcaat atattgatat tctgagttga gaatttcatc ggtgtttagt gcgggtttac
     2641 gtggcatgca attgctcctt gatgaaggta cttctattat ataggcttat cacagatgca
     2701 aaaagcggac gtttcatcaa cgtccgcttt tgttttcacg tattgcgctt agaaagccca
     2761 ttcaccttta cgcatgacag cttcgcgcgt tccatcggcc cggatgccat caatgtccat
     2821 ttggtcagac ccgatcataa agtccacatg gatgaggctg tcattgccgc cagccgctat
     2881 gaattcttct ttggtcattg atgtgccgcc cttgacgttg tagcgataag cggtacccaa
     2941 agcaaggtgg ctggacgcat tttcatcgaa gagggtgtta tagaagaggt gctttctctg
     3001 agagatggga gagctatcag gaaccaaagc agcctctccg aagcgtgagg cgttttcgtc
     3061 tgtttctaaa agtttgtgga tgttttcttc gccttgtttg gctgtgacct tgttcacacg
     3121 tccattttcg aaggtgagcg tgaattcgtc gatcatggta ccagagagaa tcagcggacg
     3181 gctggaagtg acatagcctt ctgttttttc acgatgcggc atggaaaaga cttcttcggt
     3241 tggcaggttg ggaatgtatt caataccgtt ttgagttttg ccagaagcac cgatccaaag
     3301 atggttgtct ggtaacccaa gcgttaggtc ggtgccgggg gctttgtaat ggagcccagc
     3361 atagcgcttc tcgttcaagt aatcacattg acgatgaagt ttctgtgcgt gttcgcgcca
     3421 tgcttgaact gggtctgcgg tattcacacg gcaggcgtca aaaatggctt cccataattt
     3481 ttcttgtgct tgctctacgg gtaagtctgg aaaaactttt tgcgcccaag caggggtggc
     3541 tgtagaaaca acacaccaag tttgagtatt ttcatttcta tacttggtaa caggctcgta
     3601 tttttctgaa atcgcttttc gatatctgcc gatcaaatct gggctgatgc cttctagtaa
     3661 attggggtct tgagaagcga agctcaggag cgcatctccg cgagagccat attcgtgctc
     3721 gcggtcaatt tgccactgtg gaacttcaac gaggtgttcc ggcttcgaat tttcaaaacg
     3781 gatgcgcgtc aacctttcat cgatccagcg cacatcaaca aaaggagagc ctagttgata
     3841 agctttcttt gccactaaac gaacaagctc tgcgtcaggt aacccggctg caatatttag
     3901 gcgttgttct ttatgcaggt tcaaaccaat gtttacaatg atatcggcgt atttttcaag
     3961 agaggtttgg aaggtctcgt tcatggtttc tcctttagat agacagggcg ttgaatgatt
     4021 tttaccataa gtggactcag atgaccacta actttggtga cataaaaact ccccgcctcg
     4081 tgacggggag ttgggttgat ttggttacag tgagattgcc cattcaccct gacgcatcac
     4141 cggctcatgt ttgccgtctt tggtaatgcc atcaatgtcc atttgcggag aaccgatcat
     4201 gaagtcaacg tggttgaggc tgatgttgcc tccagccgcg gtaaattcat cgtcgttcaa
     4261 ctcagttcct ccggcgagag tgaagcggta ggcgcgtcca atggcaatgt gacaggaggc
     4321 gttttcatca taaagcgtgt tgtagaagag atgtccgcgt ttgccaatgg gggatgatgc
     4381 tgggacgagg gcgatttcac cgaggcgatg tgaaccttca tcggtatcca ctaatttttg
     4441 aagggcggct tcacctttct tggctgtgac cttggtaata cgcccattct caaagtggac
     4501 ttggaagttt tcgatcaaag tgccgccata gctgagcggg aaggtggcag agatcacacc
     4561 atctgcacgg ttgcgatctg gcagagtaaa gacttcttcg gtaggcatat tagcagtaaa
     4621 agcaacacca ttctgcgcca tcgat
//
LOCUS       DAOQFT010000131         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9796_length_4632_cov_5.083024, whole genome shotgun sequence.
ACCESSION   DAOQFT010000131 DAOQFT010000000
VERSION     DAOQFT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9796_length_4632_cov_5.083024"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLR93_04645"
     CDS             <1..638
                     /locus_tag="PLR93_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNH26283.1"
                     /translation="GTRWCDICQSSLKLLDGIVCDVCGLPQDKPGVCSTCLANRPRFH
                     SLRAWTVFEKPIQPALHKLKYRRDFSMGDAIAAAMLPFVQSLNWNIDLVIPIPLGKQR
                     MHERGYNQVAMIAKPLAMGLGLQYSPHALARRKETRSQVGLSREERQKNVHDAFQASG
                     VQGKIALIMDDVSTTGSTLSSGAEALYAAGAKEVYALTVARALSHHDLRHV"
     gene            680..1243
                     /gene="raiA"
                     /locus_tag="PLR93_04650"
     CDS             680..1243
                     /gene="raiA"
                     /locus_tag="PLR93_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNH26284.1"
                     /translation="MSNKVEVQTRHIRLTERIEEYVSKKAGNLDHYLPAIEDARVELS
                     HHQAARDVNDRNVAQITVFGKGFTLRSEERADEALAAFDKALDNMQRQIERHKGKKYH
                     GRGDGRSAAEAVDDIIDDETGQLSPLFAKRKKFVMYPMNEEEALVQMRNLGHDNFFVF
                     YNAETSKINVLYRRRNGSYGLIEPELG"
     gene            complement(1287..2015)
                     /locus_tag="PLR93_04655"
     CDS             complement(1287..2015)
                     /locus_tag="PLR93_04655"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH26285.1"
                     /translation="MIETFQNLFAPPRHMILLVIAAWLGLALAERRTERHGISKEDLN
                     NITFYGLIAFVIGGRISYITQNISAFTKSPLGIVSINPNLFDPIGAIAVAFIVLIAYG
                     QRKQLNIWNIFDSLTLFFAVLAIGLGLSHLAAGSAFGLPTDLPWGINLWNATRHPTQI
                     YDTLASSIILILLWLFKPSPRPGITFLTYAAFVALAQLILQAFRANYTIIFSGLRQEQ
                     VVAWVGLLVCFILIEVRLKSQEKQ"
     gene            complement(2012..2554)
                     /locus_tag="PLR93_04660"
     CDS             complement(2012..2554)
                     /locus_tag="PLR93_04660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH26286.1"
                     /translation="MNRTQRIILYILLLIAGASWIVISGQPTGTTTVAAAAPQAGFTA
                     PDFTLKTAEGETYTLSELKGQAVLINVWATWCPPCKAEMPAIEKMYNEYKDQGFNVLA
                     INSTFQDDPLQIKPFTEEYSLTFPILLDETSDISRDYQVRSLPSSYFVNRYGIITEVV
                     IGGPMSEALLRTKIEEALKP"
     gene            2622..3380
                     /locus_tag="PLR93_04665"
     CDS             2622..3380
                     /locus_tag="PLR93_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HNH26287.1"
                     /translation="MLFTDMVFVGIDAASSHKSYTYAALDKGMNLVALADGELDDVTT
                     FLGGQSSAIVAINAPSGVNRGLVREKKKDMFKSVKPRAAGFRVAELELRDRGITVSGT
                     PSTISACSAWVQSGFALYRKLEKMGFKKYPTENTPYQILESQPHAAFCVMAEAIPQPK
                     PSLEGKIQRQLLLYEAGVRIKDPMDFFEEITRHKMLKGNWPLDMLYQPEQLDALVAAY
                     TAWLSIHKPEKVSLIGDSTEGQIALPEKNLKEKY"
     gene            3402..>4632
                     /locus_tag="PLR93_04670"
     CDS             3402..>4632
                     /locus_tag="PLR93_04670"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNH26288.1"
                     /translation="MTNLEFQNKAINTLRFLSADGVQKANSGHPGLPMGAAAMAFTIW
                     TRHLRHSPRNPKWMGRDRFILSGGHGSMLLYSLLHLTGYDLSLEDLQSFRQWGSKTPG
                     HPEYGWTPGVEVTTGPLGAGLSTGVGMAIAGAHLAATFNKPGHELLDAYIYAIVTDGD
                     LMEGVASEAASLAGHLSLGKLIYLYDDNKISIDGSTDLSFTEDRAKRFEAYGWHIQRV
                     EDGNDVEAIDAAIKAAKTDPRPSLILCRTIIGFGAPNRQGTQKAHGEPLGDEELNAAK
                     DNLGWPKEPRFLIPDDVLEFYRKAVDRGRELEADWKMRREAYARLFPDLGTEFNRRIL
                     MNKLPEGWESALPTFPADPKGMATRAASGKVINALAAKLPELIGGSADLAPSNNTKID
                     GSPAFQKDSPAGRNFHFG"
BASE COUNT         1223 a          984 c         1196 g         1229 t
ORIGIN      
        1 ctggaacaag atggtgtgat atctgtcaaa gtagcctgaa attgttggat ggtattgttt
       61 gtgatgtatg cggtctaccg caagataagc ctggtgtttg ttccacttgt cttgcgaata
      121 gaccgcgttt tcattctttg agagcttgga ccgtgttcga aaagcctatc cagccagctt
      181 tgcataagtt gaaataccga cgggactttt ctatgggcga tgcgattgcg gcggcaatgt
      241 tgccgtttgt gcaaagctta aattggaata ttgatcttgt gattccaatt ccgttgggaa
      301 aacaaagaat gcatgaaagg ggatataatc aggttgcaat gatcgcaaaa ccacttgcta
      361 tgggtttggg cttgcaatat tcacctcacg ctttggcgcg ccgaaaagag acgcgttctc
      421 aagtggggct gagtagggag gagagacaga agaatgttca tgacgctttt caagcatcag
      481 gggtgcaagg aaaaatagct cttattatgg atgacgtttc aacaactggt tcgactcttt
      541 cctctggcgc agaggctctt tatgctgcgg gtgcgaagga agtatatgcg ttgacagtcg
      601 cgcgtgcatt gtctcatcat gatttgaggc atgtatagcg cgtgtttgta acttaacaac
      661 ctaataaaag gagtatctta tgtccaataa agtggaagtt cagacacgtc atattcgctt
      721 gacagaacgg atcgaagagt atgtttcgaa gaaagcaggc aaccttgatc attacttgcc
      781 ggctattgaa gatgcgcgcg tggaactttc gcaccaccaa gctgcacgcg atgtcaatga
      841 tcgaaatgtt gcgcagatta ctgtgtttgg aaagggattt acccttcgct cagaagaacg
      901 cgcagacgaa gccttggctg catttgacaa agcattggat aatatgcagc gtcagattga
      961 aaggcataaa gggaagaagt atcatggacg tggcgatgga cgttcggctg ctgaagctgt
     1021 cgacgacatc atcgatgatg agaccgggca actttcaccg ctttttgcaa aacgcaagaa
     1081 atttgttatg tatcctatga atgaggaaga agcgttagtg caaatgcgta atttagggca
     1141 cgataatttc tttgtgtttt acaatgcaga gacgagcaag attaatgttc tgtatcgcag
     1201 gcgcaatgga agctatggtc tgattgagcc tgagcttgga taggctttca ataaataaaa
     1261 gagctgacta aagtcagctc ttttatttat tgtttttctt gggatttcag cctaacttct
     1321 ataagaatga aacatactag taagcccacc catgcaacta cttgttcctg acgaagccca
     1381 gagaaaataa tagtgtaatt agctcggaaa gcttgcaaga tgagttgtgc caaggctaca
     1441 aatgcggcat acgttaagaa ggtgatgcct gggcgtggac ttggtttgaa gagccagagc
     1501 aagatcaata tgatggaaga ggcgagcgtg tcataaattt gagttgggtg acgtgtggca
     1561 ttccataagt taattcccca cggaagatcg gtagggagtc caaatgcact gcccgcagca
     1621 aggtggctta acccaagccc aatggcgagg acagcgaaaa aaagtgtcag cgagtcaaag
     1681 atattccaaa tgttgagttg cttgcgttga ccatatgcta tgagaacgat aaaggctacg
     1741 gcaattgcac cgatagggtc gaagagattt gggttgatgg aaacaatgcc aagtggactt
     1801 tttgtaaacg ccgatatgtt ttgggtaata tacgaaatgc gcccaccaat gacaaaagca
     1861 attaatccat agaaggtaat gttattgagg tcttctttgc tgatgccgtg acgttctgta
     1921 cgccgttctg ccaatgcaag accaagccat gcggcgataa cgagcaagat catgtgacgt
     1981 ggcggcgcga ataaattttg gaacgtttcg atcatggttt caaagcctct tcaattttag
     2041 tgcggagcaa agcttcagac atggggccac cgatcacaac ttcggtgatg atgccatagc
     2101 gattgacaaa atacgatgaa ggcagtgagc ggacttgata atcacggctg atatctgaag
     2161 tttcatctaa taagatggga aaggttaagc tatattcttc agtgaacggt ttgatctgaa
     2221 gcggatcatc ttgaaaggtg ctgttgatcg ctaacacgtt aaagccctga tccttgtatt
     2281 cgttgtacat tttttcaata gcaggcattt cggctttgca tggcgggcac caagttgccc
     2341 atacattaat aagaaccgct tgccctttta gttcggagag ggtgtatgtt tcaccttctg
     2401 cggtttttag ggtaaagtct ggggcggtaa accccgcttg aggggcggct gcggcgactg
     2461 tggttgttcc ggttggctga ccggagatta cgatccacga agccccggca ataagcaaaa
     2521 gaatgtagag aatgattcgt tgggttcggt tcatgagatt ttagaagtaa aataggactg
     2581 tttgttttgt attcggtgtg ctcttaagag ataatcttac tatgcttttt acagacatgg
     2641 tttttgttgg tattgacgcg gcttcttccc ataagtccta tacgtatgct gcactggata
     2701 aggggatgaa tcttgtcgct ctggctgatg gcgagctgga cgatgtgacg accttcctgg
     2761 gtgggcaatc ttcagcgatt gttgcgatca atgcgccttc tggcgtaaat cgtgggttgg
     2821 tgcgcgagaa aaagaaagac atgtttaagt cggtgaagcc gcgtgcggct ggttttcgcg
     2881 tggcagaatt ggaattgcga gaccgcggca ttacggtttc tggtactcct tctacaatta
     2941 gcgcttgctc tgcatgggta caatctggtt ttgcgcttta tcgtaaactg gaaaagatgg
     3001 gcttcaagaa atatccaact gaaaatacgc cctatcaaat tttagagtct cagccgcatg
     3061 ctgctttttg tgtcatggct gaggcgatac cgcaaccaaa gccttcattg gaagggaaaa
     3121 ttcaacgtca attgcttctg tatgaagctg gcgttcgtat caaagacccg atggattttt
     3181 ttgaagagat cacacgccat aaaatgctaa aaggcaattg gccgttagat atgctttatc
     3241 agcctgagca attggatgca ttggtggcag cctatacggc ttggctgtcg attcataaac
     3301 ctgaaaaggt ttcccttatt ggagattcta cagaagggca gatcgcccta cctgagaaaa
     3361 atttgaaaga aaaatactaa ccgaatggta aaataccgcc catgacaaac ctcgaatttc
     3421 aaaacaaagc aatcaataca cttcgatttc tttccgctga cggcgtgcaa aaagcgaatt
     3481 ctggtcatcc cggtctcccg atgggagccg ctgcgatggc tttcaccatc tggactcgcc
     3541 atctgagaca tagcccccgt aaccccaagt ggatgggacg tgaccgcttc atcctttcgg
     3601 gtgggcatgg ttcgatgctt ctttactctc tcctccattt gaccgggtac gacctatctt
     3661 tggaagactt gcagagtttc cgccagtggg gcagcaaaac ccccgggcac cccgaatacg
     3721 gctggacacc cggcgtggaa gtaaccactg gtcctcttgg tgcgggtctc tccactggtg
     3781 tggggatggc aattgctggt gcacacttag ctgccacatt caacaaacca ggtcatgaat
     3841 tacttgacgc atacatctat gccatcgtca cagatggtga tttgatggaa ggtgtcgctt
     3901 ctgaagccgc ttcactggct gggcacctct cgctcggaaa acttatttat ctgtacgacg
     3961 ataataagat ttccatcgat ggctcaacag acctttcctt cacagaagac cgcgccaagc
     4021 gttttgaagc atatggctgg catattcagc gtgtagaaga tggtaatgat gtagaagcga
     4081 ttgatgccgc tatcaaagct gctaaaacag acccacgccc gtcgttgatt ttgtgccgca
     4141 ccatcatcgg ttttggtgct ccgaaccgac aaggcactca gaaagcacat ggcgaaccgc
     4201 taggggatga agaactgaat gctgcaaaag ataaccttgg ctggcccaag gaacctcgtt
     4261 tcttgatccc tgatgatgtg cttgagtttt atcgcaaagc cgttgaccgc ggacgtgaac
     4321 tcgaagccga ttggaagatg cgccgcgaag cgtatgcacg gctcttcccg gatcttggaa
     4381 cagaatttaa tcgtcgcata ttgatgaaca aattgcccga aggttgggaa tctgcactac
     4441 cgacattccc cgctgacccg aaaggtatgg caactcgtgc cgcttctggt aaggttatta
     4501 acgcactggc ggcaaaactc cctgagctta ttggcggctc ggcagacctt gccccttcga
     4561 acaataccaa aattgatggt tcgcctgctt tccaaaaaga ctctccggct ggacgtaact
     4621 tccacttcgg tg
//
LOCUS       DAOQFT010000132         4621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9841_length_4621_cov_5.798292, whole genome shotgun sequence.
ACCESSION   DAOQFT010000132 DAOQFT010000000
VERSION     DAOQFT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9841_length_4621_cov_5.798292"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLR93_04675"
     CDS             complement(<1..1206)
                     /locus_tag="PLR93_04675"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNH26289.1"
                     /translation="MRPKLTLLSDEIIARILAEAYELLLKPGIKVQNEEARELLRQEG
                     VVIDPETNVAQIPASLVQRALESVPREFYLYDYHGNPTIHYGGDSVHFDPGSSGITML
                     NPDTLEHDTTETEHLIRLLKVAEQIPQYDAQSTAVICHDVPKDIQDSYRLYLALLYSK
                     KPIVTGAFTNKTVQEMIDMLAMFAGSAEEARQKPRAIFDVCPAPPLIWSNFGAGNLIQ
                     LARAGIPAEIVSVPLAGAAAPVTMLGTVTQHTAECLAGITIHQLAKPGSPIVWGGAAA
                     IFDMKKGATPMGAIETAMLDCSYAQVAKTLGMPTHTYLGATDSKLVDAQAGLESGITA
                     MVGALSGINMISGSGMLDFLACHSAEKLVIDAEGIAMAKRMVMGVKQHTDTLATGFYD
                     DKINFKGGDF"
     gene            complement(1316..2767)
                     /locus_tag="PLR93_04680"
     CDS             complement(1316..2767)
                     /locus_tag="PLR93_04680"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNH26290.1"
                     /translation="MSHKKIKTISDPKLKLDILSKEDVQKIHEATLHIIENVGIRFPS
                     KRALRIWEEAGATVDHEKKIVKVKPYLIEDALKQAPPAYTLGARDPQQNLSLDGNHVH
                     LGTDGCGVEIIDIHTGMKRTTELQDVRDIARIADATEEVAFHWVPVSAQDAPVEARGL
                     HELKAVWENSTKHVQTESIYNVEEAKASMEMAILLAGSKEDLRKRPVLSLMQCTAPPL
                     GHDGGSLDAALIAAEHGVPTGFMTMTACMTTGPATMAGTLAVGNAEVIAATALLQLAY
                     PGAPVFYAAAQTASDPRTGVYTGGGPEDFLFGAATNVLADFYNIPLSMGSFATGAKEP
                     NWQAGLEGSLSTFMASVAMSDMLLGCGFLHGSRIWSYAEMMMDCEIFSIIEKMMHGIE
                     VNAETLALDAVAAVGPGGHYLAQKHTRNHMREIFMPQFLDRRPYSKWEETGEDARDWA
                     VNKARKILKEHQPDPLDEKISKEFERIIKSVEK"
     gene            complement(2814..3473)
                     /locus_tag="PLR93_04685"
     CDS             complement(2814..3473)
                     /locus_tag="PLR93_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNH26291.1"
                     /translation="MSEEIEKLFKDMAQSIIDGDSDLSVELAEKSIAMNIPPLDAITK
                     GFVIGVNYIGDQFGAGEAFLPELVMAGEAMKAAVATLEPELLKLGEARETMGKVILAT
                     VEGDIHEIGKTLVGTMLSASGFEVTDLGVDQPADKIIGKALEIDAQIIGLSALLTTTM
                     VRQREIIEELDKENLRPRIKVMVGGAPITRDWATKIKADGTSEDAVGAVALAKQLVGK
                     A"
     gene            complement(3491..4225)
                     /locus_tag="PLR93_04690"
     CDS             complement(3491..4225)
                     /locus_tag="PLR93_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNH26292.1"
                     /translation="MAAKELSHKPLKEEIYDALHRQIIAGRYGPGDWLRQEDIASQMG
                     VSMTPVREALDLLVAKGIAERVPYRGVRVREMSPEDVVEAYGMRLFLEAMVARETALN
                     ITPEQVQKLENIMAEMDRHVELNEMPQERQLSREFHSAIAEASGNKLLVELYSIVSNA
                     FPDWLLYEALYRQPELVSSSVSQTHDEHAAILNAFKKHDPDLAVKVSLEHVMESGRWL
                     ENYRNIPAKLLREKEKQVSHLIKKSK"
     gene            complement(4277..>4621)
                     /locus_tag="PLR93_04695"
     CDS             complement(4277..>4621)
                     /locus_tag="PLR93_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26293.1"
                     /translation="TLAHFQRAQPPFKEEADLILASFSEEAEPNPDEPESATNRKPSS
                     PEIVWEAVQDALFSEKPKLRYLVGTHWEGNRVLNALMSKLLDENDNPQHNYSRDELIA
                     LLDKHLTERNAS"
BASE COUNT         1031 a         1139 c         1178 g         1273 t
ORIGIN      
        1 gaagtcgccg cctttgaagt tgattttgtc atcataaaag ccggtggcaa gagtatccgt
       61 atgttgtttg acgcccataa ccatgcgttt tgccatggca atgccttctg catcgatgac
      121 gagtttttcg gcgctatggc aggcaaggaa gtccagcata ccggagccgg agatcatatt
      181 gatgccgctg agtgcgccaa ccatagctgt gatgccgctt tcaagaccgg cttgcgcatc
      241 gacaagtttg gagtctgtgg caccaagata ggtgtgagtg ggcatgccaa gagtctttgc
      301 aacttgtgca taggaacaat caagcatggc ggtttcaatg gcacccatgg gggttgcgcc
      361 tttcttcatg tcgaagatgg cggctgctcc accccaaacg ataggggagc cgggttttgc
      421 caattgatgg atggtgatgc ccgccaaaca ttctgcggtg tgctgggtga ctgttccgag
      481 catggtgacg ggtgcagccg caccagccaa cggaaccgag acgatctccg ctgggatgcc
      541 cgcacgtgcc agttgaatta agttacctgc cccaaagtta gaccagatca agggcggtgc
      601 tgggcaaaca tcgaaaatgg cgcgcggttt ctggcgtgct tcctctgcgc tgcctgcgaa
      661 catagcaagc atatcgatca tctcttgcac ggtcttgtta gtgaatgcgc cagtgacgat
      721 aggtttcttg gaatagagca acgccaaata aaggcgatag gaatcttgaa tatctttggg
      781 aacgtcgtga cagatcacgg cggtagattg cgcgtcgtat tgtggaattt gttccgctac
      841 tttcaataag cggatgaggt gttcggtctc ggtggtgtcg tgttcaagcg tatcggggtt
      901 gagcatggtg atgccagaag agcccgggtc aaagtggacc gagtcaccgc cgtagtgaat
      961 agtggggttg ccatgatagt cgtagagata gaactcgcgc ggcacgctct ccaaggcacg
     1021 ttggacgagc gaagccggaa tttgcgctac attggtttca gggtcaatga caaccccttc
     1081 ttgcctaagt agttcgcgtg cttcttcatt ttgaactttg atgccgggtt tgagcaaaag
     1141 ctcgtaggct tctgccaaaa tgcgggcaat gatctcgtcg ctgagcaggg ttagctttgg
     1201 tctcatgaga cacctctaat tggaatttct ctttcgtaaa gagatataaa acgagtaacg
     1261 agggcaagaa atgagtcttc tcacacctcg ttactcgttt ctcatttctt gtgcattact
     1321 tttcgaccga cttgataatt ctttcaaact ctttgcttat tttttcatcc aacgggtcgg
     1381 gttggtgttc tttcaatatt tttcgagcct tgttaactgc ccaatctctt gcatcttcac
     1441 cggtttcttc ccatttggaa taagggcgac ggtctaagaa ttgtggcatg aagatctcac
     1501 gcatatggtt gcgagtgtgt ttttgggcga ggtaatgacc gcccggaccc accgcagcaa
     1561 ccgcatccaa tgcgagggtt tcagcattga cttcaatgcc gtgcatcatc ttttcgatga
     1621 tggagaaaat ctcacagtcc atcatcattt cggcataaga ccatatgcga ctgccgtgta
     1681 agaaaccaca tccaagaagc atgtcagaca ttgccacgct tgccataaat gttgaaaggc
     1741 ttccctcaag cccagcttgc caatttggct cttttgcgcc ggttgcgaag gagcccattg
     1801 aaagcggaat attgtagaaa tctgccaaaa cattggtagc cgcaccgaag agaaagtctt
     1861 ccggtccgcc gccggtgtag acgcccgttc gcgggtctga agcggtttga gcggcggcgt
     1921 aaaagacggg cgcgcctggg tatgccagtt gtaaaagggc ggtggcggca atgacttccg
     1981 cattgccgac ggcgagtgtg ccagccattg tggcgggacc ggtcgtcata caggctgtca
     2041 tggtcatgaa gccggtcggc actccgtgtt cagcggcgat taaggctgcg tcgagtgaac
     2101 caccgtcgtg cccgagagga ggcgcagtgc actgcatcaa cgaaaggacg ggacgcttgc
     2161 gcaaatcctc tttgctgcct gcgagcagaa tcgccatttc catcgaggct ttggcttctt
     2221 ctacgttgta aatggactcg gtttgcacgt gcttggtgga gttttcccac accgctttga
     2281 gttcgtgcaa tccgcgtgct tcaacgggag catcttgtgc cgaaacaggc acccagtgaa
     2341 atgccacttc ttctgtggca tctgcaatac gggcaatgtc gcgtacatct tgtaactcgg
     2401 tagtgcgctt catgccggtg tgaatgtcga tgatctctac accacaaccg tcggtgccaa
     2461 gatgaacatg gttgccatcg agtgagagat tttgttgtgg gtcacgcgct ccaagtgtat
     2521 aagcaggtgg agcctgtttg agcgcatctt cgatcagata cggtttgacc ttgacgattt
     2581 tcttctcatg gtcaactgtt gcaccggctt cttcccagat gcgcaatgct cgttttgaag
     2641 gaaaacgtat gccgacattt tcaatgatgt gcaacgtcgc ttcgtgaatt ttttgcacat
     2701 cttctttgct tagtatgtcc aatttgagtt ttggatcact aatggtcttg atttttttgt
     2761 gagacataga gccctcccga ggctatgtct ctcttctctc tctgattgtt tgattatgct
     2821 tttccgacta attgtttcgc caatgccact gcgccgactg catcttcgga agtgccatct
     2881 gctttgattt tggttgccca gtcgcgggtg atcggtgccc cgccgaccat gaccttgata
     2941 cgcgggcgca gattttcctt atcgagctct tcgatgatct cgcgctgacg caccatggtg
     3001 gtggtgagca atgcgctcaa accgataatt tgtgcatcga tttccaacgc cttgccaatg
     3061 atcttatctg cgggctggtc tacgcccaag tcggtcactt cgaagccaga ggcgctgagc
     3121 atggtgccaa ccagggtctt gccaatttcg tgaatgtcac cttctacggt ggcgagaatg
     3181 accttgccca tagtctcgcg ggcttcaccg agtttgagca attcgggctc gagggttgct
     3241 acagcggctt tcatcgcttc acctgccata accaattctg gcaaaaaggc ttcgcctgcg
     3301 ccgaactggt caccgatgta attgacgccg atcacaaatc cttttgtgat ggcatcaagc
     3361 gggggaatat tcattgcaat agatttttcg gcgagttcca ctgaaagatc agagtctccg
     3421 tcaatgatgc tttgtgccat atccttgaat aatttctcga tttcttctga catactgcct
     3481 cctgatgaat ttacttggat tttttgatca agtgcgaaac ttgtttttct ttttctctta
     3541 ataactttgc cgggatgtta cgataatttt ccaaccatct gccagattcc atgacatgct
     3601 ccagagagac ttttacggct aaatcagggt catgtttttt gaacgcattc aaaatggctg
     3661 catgttcatc gtgagtctgt gagacactgc ttgaaactaa ttcaggctga cgataaagcg
     3721 cttcgtacag taaccaatct ggaaatgcgt ttgagacgat ggaatacaac tctactaata
     3781 atttatttcc tgatgcctct gcaattgcgc tatgaaattc acggctcagt tggcgttctt
     3841 gaggcatttc gttcaactct acatggcgat ccatctctgc catgatgttt tcaagctttt
     3901 gcacctgctc aggcgtaata ttaagcgcgg tttcccgagc caccatcgcc tcaagaaaga
     3961 ggcgcatgcc atacgcctcc accacatctt ctggcgacat ttcccgcaca cgcacgccgc
     4021 gataggggac acgctctgcg atgccttttg cgaccaaaag gtcaagcgcc tcgcggacgg
     4081 gggtcatgct gactcccatc tggctagcga tatcttcctg cctcagccag tcgccaggac
     4141 cgtaccgccc cgcaatgatc tgccggtgca aggcatcgta aatttcctct ttcaagggtt
     4201 tatgtgaaag ctcttttgct gccattacca taattgtaca ttatatataa tctagacggt
     4261 tttcggaagt gcctaactaa gaagcgtttc gctctgtcag atgtttatct aacaatgcaa
     4321 tcaattcatc ccgtgaatag ttatgctgtg ggttgtcatt ttcatccaat agttttgaca
     4381 tcaaagcgtt aagcacgcga ttgccttccc aatgtgtgcc cactaaatag cgcagttttg
     4441 gtttttcgct aaacaaggca tcttgtacag cttcccaaac aatttcagga gaagatggtt
     4501 tgcgattcgt ggcagactcc ggctcgtctg gattcggttc cgcttcctct gaaaaactcg
     4561 caaggataag atccgcctct tccttaaacg gaggctgggc acgctgaaaa tgagccagag
     4621 t
//
LOCUS       DAOQFT010000133         4607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9889_length_4607_cov_6.423989, whole genome shotgun sequence.
ACCESSION   DAOQFT010000133 DAOQFT010000000
VERSION     DAOQFT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9889_length_4607_cov_6.423989"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLR93_04700"
     CDS             <1..338
                     /locus_tag="PLR93_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNH26294.1"
                     /translation="EDAFPFGRDESEVRLGRAGRYAFGFYEKHPCYAPWSHTLIDFNG
                     NVYVCCMTREKIPPLGNIIHQSFREIWEGAAYRQVRLKMHPPALKPCARCDDFIDQNK
                     SIWETIGPY"
     gene            complement(335..943)
                     /locus_tag="PLR93_04705"
     CDS             complement(335..943)
                     /locus_tag="PLR93_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26295.1"
                     /translation="MNVLLLHGQGRTTNAMRLLGFRLGRLGHPVQYFRYYTRSETFEG
                     IVNRFVQWVNTVNDGQPYAIVGHSMGGLIARAGLPLLKNLPVHLFLLASPNRPPKLAP
                     YVQNNFLYRYLTTDCGQLVLNSNFYQKLPLPNIPATVIAGSAGPRVGWLPYGFEPNDG
                     ALSVQDASMGDDYELIQVPSIHTFIMNSKLTLHYIEQILNHS"
     gene            complement(949..1230)
                     /locus_tag="PLR93_04710"
     CDS             complement(949..1230)
                     /locus_tag="PLR93_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26296.1"
                     /translation="MAKCEMVVGYLVPHRCDNPALGTCVKCGRGFCDEHTNATKEGRV
                     CLACQQGLEMPVALPIAASTFTPADMVLFGSTSSFDDNDDNSDMFSDLS"
     gene            1242..2399
                     /locus_tag="PLR93_04715"
     CDS             1242..2399
                     /locus_tag="PLR93_04715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH26297.1"
                     /translation="MKIALLSEKYTPDLGGLAISTGRLGEMLAAAGHDIRLFAPTSNL
                     SPTIKQTQRSSGVNVTRFGAHKRVDNTLVDWFELIVEEHKREPFDLIHAYFLPMAGFV
                     GTYAGKYLSIPSVVSIRGNDIERAAFDPSKFSHMMYALQNASAVTTNASILAKKAKAF
                     FDREAHIIPNGIDTERFKPMEKNSVLAEALGLVDEKKKEERKFVMGFVGELREKKGLA
                     TLLSGYAQITKTMPVSLLIVGEVREGEDKKYFEEFKSNNPQLSITVTGHVPHKDLPAY
                     YSLMDVFVHPSLRDGMPNAVLEAMACGVPVIATPVGGALDVLEDGVNGFFVDVNDATQ
                     LAEKIAKTLKPLKELESVRRSARETVLHHCTLEKELQANLKIYANLGVTTK"
     gene            2396..3814
                     /gene="aspA"
                     /locus_tag="PLR93_04720"
     CDS             2396..3814
                     /gene="aspA"
                     /locus_tag="PLR93_04720"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNH26298.1"
                     /translation="MKHRLEHDLLGEREIPNEVYYGVHTLRALENFPISKIPISNYPS
                     LVNALACVKEACAKANLELQLLEPEKANAVIKACEDVRGGALHDQFVVDVIQGGAGTS
                     TNMNANEVIANRGLEILGHGRGEYKFLHPLEDVNMSQSTNDVYPTAVKVALRFLINDL
                     IDGMEVLRLAFAAKAEEFKDVLKMGRTQLQDAVPMTLGQEFSTYAVMLEEDQQRLREG
                     ALLIQEMNLGATAIGTGINAHPEYAALARKYLSEITGIEYITAGNLVEATQDVGSFVQ
                     LSGVLKRVAVKLSKICNDLRLLSSGPRTGLGEINLPAVQAGSSIMPGKVNPVIPEVVN
                     QIAFLVIGNDVTVSFAADGGQLQLNAFEPIIAHSLFDSLIYLRNGCITLAERCVKGIT
                     ANHNRLEEHMNRSVGIVTALNPFIGYENASSVAKEAYTSNKSVTEIVLERGLLTKAEL
                     DEILKPEVLTKPRWIKKESGQK"
     gene            3817..4326
                     /locus_tag="PLR93_04725"
     CDS             3817..4326
                     /locus_tag="PLR93_04725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNH26299.1"
                     /translation="MGVLVRSETASDIEAISQVTYAAFLGKFSDHPTEHLIVNGLRNA
                     GMLTLSLVAEVDGNLVGHVAFSPVTIAEKFLDWYGLGPISVVPGLQKQGIGSKLILDG
                     LARLRDLGAKGCVLEGSPEYYSRFGFKPRVGLTYHAAPAPELFMALPFYDEVPTGQVE
                     YHKAFYISA"
     gene            4363..>4607
                     /locus_tag="PLR93_04730"
     CDS             4363..>4607
                     /locus_tag="PLR93_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNH26300.1"
                     /translation="MSTVFDSTKTVKNELGKQNYIASDEIATIVYLAQKLGKPLLAEG
                     PAGVGKTELAKAIASATGRELIRLQCYEGLDESKALY"
BASE COUNT         1292 a         1057 c         1136 g         1122 t
ORIGIN      
        1 atgaagatgc ttttcccttt gggcgagacg agtccgaggt ccgcttggga cgcgctggac
       61 ggtatgcatt tggcttctat gaaaagcatc cctgctatgc accatggtca cacacactga
      121 tcgatttcaa tggcaatgtc tatgtgtgtt gtatgacacg cgaaaaaatc cctccattgg
      181 gaaacatcat ccatcaatca tttagagaaa tttgggaagg cgccgcctat cgccaggtgc
      241 gcttgaaaat gcatccgcct gcgctgaaac cttgcgctcg ttgcgatgat tttattgacc
      301 agaataaaag tatctgggaa acgatcggtc cttactaact atggttgaga atctgctcaa
      361 tatagtgaag tgtcaacttt gagttcatta taaaggtatg aatgctcggc acttggatca
      421 actcataatc atcacccatt gaagcatctt gcacgctcaa tgccccatca ttgggctcaa
      481 agccataagg caaccaaccg acacgtggtc cggcagagcc ggcgatgacc gttgcaggga
      541 tattcggtaa tggcaatttt tgatagaagt tcgagtttag aactaattgt ccgcaatctg
      601 tagtgagata cctgtagaga aaattatttt gaacataagg tgccaactta ggcggacggt
      661 tcggcgaagc cagtaaaaac aaatgaactg gcaggttctt gagcaaaggc aaaccggctc
      721 gggcaatcaa cccgcccatc gaatgcccca cgatggcata gggttgccca tcattcacag
      781 tgttgaccca ctgcacaaaa cggtttacga tgccttcaaa tgtttcgcta cgggtgtaat
      841 atcgaaagta ttgcacagga tgcccaagcc tgccaagccg aaagccaagc agccgcatgg
      901 cattggtcgt ccgcccttgc ccgtgaagta aaagaacgtt cacaaacgtt agctcaagtc
      961 tgagaacatg tcgctgttat catcattatc gtcgaaggaa ctggtggagc caaacaatac
     1021 catatccgcc ggagtaaagg tcgaagcggc aatcggtaaa gcaaccggca tctcaagacc
     1081 ttgctggcaa gcaagacaaa cacgcccttc ttttgttgcg ttcgtatgct cgtcacaaaa
     1141 cccgcgtcca cacttaacgc acgtccccaa tgctgggtta tcacaacgat gcggaaccaa
     1201 ataaccaaca accatttcac actttgccat cgagacacct catgaaaatt gctttgctta
     1261 gcgaaaagta tacaccagac cttggcgggc tcgcaatctc aacaggacga ttgggggaga
     1321 tgcttgccgc ggctggacat gacatccgcc tcttcgcccc gacttcaaac ctgtcaccca
     1381 ccatcaagca gactcaacgc tcaagcggag tcaatgtcac tcgcttcggc gcacacaaac
     1441 gcgtggacaa tacattagtg gattggttcg agttgatcgt ggaagaacac aagcgcgagc
     1501 catttgatct gatccatgcc tacttcctcc ccatggcggg ctttgtcggt acctatgctg
     1561 gaaaatacct gagcattccc agtgtcgtct ccattcgtgg aaatgacatt gaacgcgccg
     1621 ccttcgaccc aagcaaattt tcacatatga tgtatgcctt acaaaatgca agtgctgtaa
     1681 caaccaacgc cagcattttg gcaaagaaag caaaagcctt ttttgaccgt gaggcacata
     1741 tcattccgaa tggaattgat acagaacggt ttaaaccgat ggaaaaaaat tcagtcttag
     1801 ccgaagcact gggcttggtg gatgaaaaga agaaagaaga aagaaaattt gtcatggggt
     1861 ttgttgggga attaagagaa aagaaggggt tggctactct cctttctggc tacgcgcaaa
     1921 ttacaaaaac catgccggtt tctctgctta ttgttggcga agtacgagaa ggcgaagata
     1981 aaaagtattt tgaagaattc aaaagcaaca atccgcaact aagcattact gttactggac
     2041 atgtccccca caaagactta cctgcatatt attccttgat ggatgttttt gtacatccgt
     2101 cactgcggga tgggatgcca aatgccgttc tcgaagccat ggcatgcgga gttccagtca
     2161 ttgcaacacc agttggtgga gcgttagatg tgttggaaga tggtgtgaat ggatttttcg
     2221 tagatgtaaa tgatgcaaca caactagcag agaaaatagc taaaacgcta aaaccgctta
     2281 aagaattaga atcagttcgt aggtccgcac gagaaacggt tctacatcac tgtacgcttg
     2341 agaaagagtt gcaagcaaat ctaaaaattt atgcaaacct tggagtcaca acaaaatgaa
     2401 gcatcgactt gagcatgacc ttttgggcga acgtgagatc ccgaatgagg tgtattacgg
     2461 ggtgcatacg ttgcgtgcgc tggaaaattt cccgattagt aaaattccga tcagcaatta
     2521 tccaagtctt gtaaatgcat tggcatgtgt caaggaggcg tgtgcaaaag cgaatttgga
     2581 attgcaattg ctagagcctg aaaaagccaa tgccgtcatt aaagcttgtg aagacgtgcg
     2641 tggcggagca ctacatgacc aattcgtggt tgatgtaatt caaggcggcg cgggcacgtc
     2701 cacgaacatg aatgcgaatg aagtgattgc caatcgcggg ttagagattc ttgggcatgg
     2761 acgtggggaa tataaattct tgcatccgct tgaagatgtg aatatgagtc aaagtacaaa
     2821 tgacgtttac ccaactgctg tcaaagtagc gttacggttt ttgatcaatg acttaattga
     2881 cggcatggaa gtattacgtc ttgcgtttgc ggcaaaagcc gaagaattca aagatgttct
     2941 aaaaatggga cgcacccagt tacaagacgc ggttcccatg acgctcgggc aggaattcag
     3001 tacctatgca gtgatgcttg aagaagacca acagcgtctt cgtgaagggg cattgctcat
     3061 tcaagaaatg aacctcggcg cgactgccat tggcacgggc attaatgcac accctgaata
     3121 tgctgccctc gctcgcaaat atctaagtga aatcacagga attgaataca tcactgctgg
     3181 aaatctcgtg gaagctaccc aagatgttgg ctcgttcgta caactctcag gtgtgcttaa
     3241 gcgtgtggca gtaaaactct ccaagatctg caatgacttg cgattgctct cctccggtcc
     3301 gcggacgggg ctgggcgaaa tcaacctccc tgcagtgcaa gccggttcga gcatcatgcc
     3361 gggcaaagtc aatccggtca ttccggaagt ggtgaaccag attgcttttc tcgtcatcgg
     3421 caatgacgtc actgtctctt tcgcagccga tggcggacaa ttacaactca atgccttcga
     3481 gccgattatc gcacacagtc tatttgatag tctcatttac ctccgcaatg gctgtattac
     3541 tttagctgag cgctgtgtaa aagggatcac cgccaaccat aaccgtcttg aagaacatat
     3601 gaaccgctcg gttggaatcg ttacagcgtt gaatccgttc atcgggtacg aaaatgcaag
     3661 ctcggtggcg aaagaggcgt acacatccaa caagagcgtt accgaaatcg tactggagcg
     3721 cggtctgctc acaaaggcag agcttgatga aatcctcaaa ccggaagtgc tcaccaagcc
     3781 gcgttggatt aagaaagaat ctggacaaaa gtaagtatgg gcgttctcgt tcggtctgaa
     3841 acagcaagtg atattgaagc catttcacaa gtcacgtacg ctgcgtttct tggcaagttc
     3901 agcgaccatc ccacggaaca tctcatagtg aatggtttgc gtaatgcagg catgctcacc
     3961 ctttcactcg tggctgaagt ggacggaaac cttgttgggc atgtagcgtt ctcacctgtg
     4021 accatcgctg aaaagttttt ggattggtat gggttgggtc cgatctcagt tgtgcctggt
     4081 ttgcaaaaac aaggcatcgg ctcaaaattg attttggatg gattggcaag attgcgagat
     4141 ctcggagcga aaggatgcgt gctcgaaggc agcccggaat attactcgcg cttcgggttc
     4201 aaaccgagag ttggtcttac ttatcatgcc gcacctgccc cggagctttt tatggcgctt
     4261 cctttttacg atgaagttcc aactgggcaa gtggagtatc acaaagcgtt ttatataagt
     4321 gcatgacgat ttactacgtt tcgctgtcga ataaggagag agatgagtac tgtttttgat
     4381 tcaacgaaaa ccgtaaagaa cgaacttggc aaacagaatt acattgcctc tgatgaaatt
     4441 gcaaccatcg tatatcttgc tcagaagcta ggcaagccat tgctagcgga gggtccggct
     4501 ggcgtaggca agacggagtt ggcaaaagcg attgcttcgg caacgggacg tgaactcatc
     4561 cgcttgcaat gctatgaagg cttggacgag tccaaagcgt tgtacga
//
LOCUS       DAOQFT010000134         4606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9895_length_4606_cov_8.005713, whole genome shotgun sequence.
ACCESSION   DAOQFT010000134 DAOQFT010000000
VERSION     DAOQFT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9895_length_4606_cov_8.005713"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..2061
                     /locus_tag="PLR93_04735"
     CDS             328..2061
                     /locus_tag="PLR93_04735"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNH26301.1"
                     /translation="MQARKLTRRDFLKFGGALAATSALAACAPGEENAITQPFEGTTT
                     LQNPVITPEAQTSLPPVSMAILALSRLTFGIRPGDVDAFNALGATDDERLSAFVTQQL
                     NPDSINDSEFEARYANAGFETLHKSHEQLYADHIASNPYDSNDDAYWQWYSKPTYELV
                     DATILRAVYSKKQVVELLADFWHNHFNVYFWQDDGVPLLVSYNRDVLRAHMLGNFRQM
                     LEAVARHPSMLYYLNQNNSSDAGPNENFARELFELHTLGAENYLGVRDPNTVEKDANG
                     IAVGYVDNDVYESARCLTGWRVDDDLGDWEDGVEKTGNFLYYKPWHDRFNKLVLGKYI
                     PADQEDMKDAHDVLDLLAYHPGTARHIARKLCTRFISDVPSESIVQAAANTFIQFKDA
                     PDQLKRVMETILLSAEFKASWGGKVKRPFEAAISMIRALNSETVRISGKLNWMLSLMG
                     QAIFERRTPDGYPDTRDAWANSMSLLYRWNLAVSLAENWMDDDDTGFKIASDVYGQTP
                     AEIRSASSLADYWIQRILNRTLPDESRNAIIAVMAQEYGWDETLPEDHVHYVLPAMVE
                     VILMSPDFQWR"
     gene            2066..3289
                     /locus_tag="PLR93_04740"
     CDS             2066..3289
                     /locus_tag="PLR93_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNH26302.1"
                     /translation="MTTLLPRRQFLQGCSAAIAAMAGAKLTNLAFAKQDNPSDILVVI
                     FLRGGWDALNVVPPIDGDDRGFYEKARPDLKISSLLRLNDQFGLHPAMSPLYDLYQAG
                     KAGIIHSVGLNYDTRSHFDAQDYIELGTPGSKSTTSGWITRHLQTTGISSILPVVSTT
                     GQPMSLLNFVPTVSVSTPDEFSLWDNGLRDSHMNALRQLYAGESLIHRAGSRTLDALD
                     IVRPLNGNTYQPANGAYYNDDELGNQLKTIAQMIKLDAGLRVAAVDFGGWDTHEYQNS
                     GNEGYMADLLNSLASGLANFYLDLDSGYTEKLSVVVISEFGRRLVQNDSYGTDHGHGS
                     VMLTLGGNVNGGQVFGSFPGLHNDQLYDHADLAITTDYRSVLGELLSKRMGNADLESV
                     FPGFSGNYNPLGIYR"
     gene            complement(3334..4299)
                     /locus_tag="PLR93_04745"
     CDS             complement(3334..4299)
                     /locus_tag="PLR93_04745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH26303.1"
                     /translation="MQSLTDISKRDLEILFDHIQAAIALVDRDGNLISWNSAFDAGKR
                     QYPEVTNLKDLFLQRDAESLRMKLAEGVANHWAGEFAITETDAAVLCDFWLMPTVTEH
                     MLFIAERIESEADAENMVEKLRKQVKLFQVESEVSKKLARNKQIEMEAVLAQAQEVAH
                     VDPLTFLPNRRTIIKDLQSEVMRAERYHSKFCISVVDIDKFKSINDTYGHPVGDEVLR
                     HVALQLRDGIRHPDVVGRYGGEEFIILLPNSDNNAAAEQASRLCRQIRSSVVRSKDHD
                     IQVTISVGIAQFHIGEDSWHSLLKRADNAMFEAKSRGRDRWVVGD"
     gene            complement(4359..>4606)
                     /locus_tag="PLR93_04750"
     CDS             complement(4359..>4606)
                     /locus_tag="PLR93_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26304.1"
                     /translation="VEDFEEDESPLQAEVEARLAARVCKRLAVKGGQVLTSDEQKSLL
                     LDLENCQSPRTCPHGRPTMIHLSVDTLERQFGRRGAR"
BASE COUNT         1157 a         1207 c         1068 g         1174 t
ORIGIN      
        1 atgcgcatca cctgcaggac gtcgagcgcc tcttgcagat cgttcaattc ccccagggag
       61 acctcggagg tgttggtctc cgcctcaggc ggatgtggga agcatccgcc gaggatggtg
      121 agtacgaggc tgatcgtctt gggccctgct caacccggtg ccgtctatgc aagaagtaaa
      181 acagcatata aaagaagcct tttgtacagt ttttgaaaac tccatgtaaa ccacgaaaac
      241 ttattcggta cttaagcggg atatttataa taagaaccga actgtataag taaaaaagcc
      301 tgaaatccat tttttgttgg aggtcttatg caagctagaa aattaacgcg acgtgatttt
      361 ctaaagtttg gcggagcctt ggcagcaaca tctgctttag cagcttgtgc gccgggagaa
      421 gaaaacgcga tcacgcagcc ttttgaaggg acaacaactc tacaaaaccc ggttattaca
      481 cccgaagcac aaacgtctct tccccctgtt tcgatggcga tcctagcttt gagtcgcctt
      541 acatttggca ttcgccccgg tgacgtggat gctttcaatg cccttggcgc aacagatgac
      601 gaacgtcttt cagcttttgt aactcaacaa ctcaacccag atagcatcaa tgacagtgag
      661 tttgaagcac gttatgccaa tgccgggttt gagaccttgc ataaatcgca tgaacagtta
      721 tacgcagacc acattgccag caatccatac gactctaatg acgatgcata ctggcaatgg
      781 tatagcaaac ccacttatga attagtagat gccaccatct tacgagccgt atacagcaaa
      841 aaacaagttg tggaacttct cgcagatttc tggcataacc atttcaatgt ctatttttgg
      901 caggatgatg gcgtgccact gctggtttca tacaaccgcg atgtgttgcg tgcccatatg
      961 cttggcaact tccgccaaat gttggaagcc gtcgcaaggc acccttctat gttgtactac
     1021 ttgaatcaaa acaacagcag cgacgcaggt cccaacgaga atttcgcacg cgaattattt
     1081 gagcttcaca cactcggcgc agagaattat ctgggcgtac gtgacccaaa cacggttgag
     1141 aaagatgcga atggcattgc cgtcggctat gttgataacg acgtctacga gtctgcgcgc
     1201 tgtctcaccg gctggcgggt ggatgatgat ctcggtgatt gggaagatgg tgttgaaaag
     1261 acaggcaact tcctgtatta caaaccctgg catgaccgtt tcaacaaact cgtgctcggc
     1321 aaatacattc ctgccgatca ggaagatatg aaagacgctc atgatgtact cgacctttta
     1381 gcctatcacc cgggcacggc tcgccacatt gcaagaaaac tttgcacacg cttcatttcc
     1441 gatgtgccat ccgagtcgat tgttcaggct gcagccaata cttttattca attcaaagat
     1501 gcaccagacc aactcaagcg tgtaatggag accatcctgc tttcagcgga gtttaaagcc
     1561 agttggggcg gaaaggtcaa acgtccgttt gaggcggcaa tatccatgat acgtgcgctt
     1621 aactcagaaa cggtgcgcat ttcaggcaaa ctcaactgga tgttgagtct gatgggtcaa
     1681 gccatctttg aacgccgcac acctgatggc tatcccgaca cccgcgatgc atgggcaaac
     1741 tccatgtcgc tcttgtatcg ctggaatctg gcggtaagtt tggcagaaaa ctggatggat
     1801 gatgacgata ccggcttcaa aatcgcttcg gatgtttatg ggcaaacccc ggcggagatt
     1861 cgcagcgcct cttctctcgc cgattattgg attcaaagaa tcctgaaccg tacgctaccc
     1921 gatgaaagcc gcaatgccat catcgcagtg atggcacaag aatacggttg ggacgaaacc
     1981 ctgcctgaag atcacgtgca ctatgttctg cctgccatgg tggaagtgat tttaatgagc
     2041 cccgattttc aatggagata acccaatgac cactttactt ccacgccgtc agttcctgca
     2101 aggatgttca gccgccattg ccgccatggc gggcgcaaag ttgaccaacc tcgcttttgc
     2161 taaacaagat aacccaagcg atatcctcgt cgtcatattt ctacgcggcg gttgggatgc
     2221 gctcaatgtt gttccgccta ttgatggaga tgaccgtggc ttttacgaaa aagcccgccc
     2281 cgatctgaag atatccagtc tattgcgctt gaatgaccaa tttggtctgc accctgccat
     2341 gagtccgtta tacgatctgt atcaagccgg taaagcaggc atcattcact ctgtggggtt
     2401 gaattacgat acgcgcagtc acttcgacgc tcaagactat attgaattgg gaacgcccgg
     2461 ttccaagagc actacctcag ggtggatcac ccgtcactta cagacaaccg gcatttcttc
     2521 gatcttgcca gtggtttcca ccacagggca gcctatgtcg ttgttaaatt ttgttcccac
     2581 agtcagtgtg agcacgcctg atgaattctc attatgggat aacggcttgc gcgactcgca
     2641 catgaatgcg ttgcgccaac tctacgccgg ggaaagcctc attcaccgtg ccggctcgcg
     2701 gacattggat gccttggata tcgtccgccc gttgaatgga aatacatatc aacccgccaa
     2761 tggcgcttac tacaatgatg atgaacttgg caatcaacta aagaccattg ctcaaatgat
     2821 caaactcgat gccggcttac gcgttgctgc cgtggacttt ggcggttggg atacgcacga
     2881 atatcagaac agcggcaatg aaggctacat ggcagatttg ctcaacagtc tggcgtctgg
     2941 gttggcaaat ttttacctag acttagactc tggatacacc gagaaattat cggttgttgt
     3001 cattagtgaa tttgggcgtc ggcttgttca aaacgactcc tacggcaccg atcatggaca
     3061 tggcagtgtg atgcttacgc ttggcggtaa cgtcaatggc gggcaagtct tcggttcctt
     3121 ccccggtttg cataatgacc agctatacga ccatgctgac cttgccatca ctacagatta
     3181 tcgctccgta ttgggagagt tactttccaa acgcatgggc aatgccgacc ttgagtctgt
     3241 cttccccggc ttttctggca attacaaccc gctcgggatt tacagataac tatttgaaaa
     3301 agagcctatc tcaatgagac aggctctttt tatttaatct ccaacgaccc agcggtcacg
     3361 tccgcggctt ttggcttcga acatggcatt gtctgcccgt ttgagcaagc tatgccatga
     3421 atcctcgccg atgtgaaatt gagcaatccc cacactgatg gtcacctgaa tatcatggtc
     3481 tttcgatcgg accacagaac tgcgtatctg tctgcacaaa cgtgaggctt gttcagccgc
     3541 agcattgtta tctgagtttg ggagcagaat gatgaactct tcgccgccat aacgtcccac
     3601 cacatcgggg tgacggatgc catctcgcaa ttgaagtgcc acatggcgta acacttcatc
     3661 ccctactggg tgcccatacg tatcattaat ggacttaaat ttatcaatat ccacaactga
     3721 aatacaaaac ttagagtgat atcgttcagc acgcatcact tcgctttgca agtctttgat
     3781 gatagtacgg cgattcggca ggaaggtcag tgggtcgaca tgcgctactt cttgagcctg
     3841 cgccaagacc gcttccattt caatctgttt attgcgtgct agtttttttg aaacttcgct
     3901 ttccacctga aacaatttca cttgcttgcg gagcttttcc accatgttct ctgcatcggc
     3961 ttccgattcg atccgttcgg cgatgaagag catatgttcg gtcacagtcg gcatcaacca
     4021 gaaatcgcac aacacagcag catcagtctc agtaatggca aactccccag cccaatgatt
     4081 ggcaacacct tctgccagtt tcatacgcag gctttcagcg tcgcgctgca gaaaaaggtc
     4141 ctttaagttc gtgacctctg ggtattgccg tttccccgcg tcaaatgcag aattccacga
     4201 aattaaattt ccgtcccgat ccaccaaagc aatcgctgcc tgaatgtgat caaacagaat
     4261 ttcaaggtca cgtttggaaa tatctgtcaa actttgcata gcataattgt agcgtattcg
     4321 cctacccttt tatatgtcct tacaaaatta ccaactgatt aacgcgcccc tctgcgtcca
     4381 aattggcgtt ccaacgtatc gaccgataag tggatcatgg tcggtctgcc atgcggacac
     4441 gtccgcggcg attggcaatt ctccaagtca agcagcagac ttttctgctc gtcggatgta
     4501 agcacctgcc cgcctttgac cgccagccgt ttacagaccc gcgctgccag ccgcgcctcc
     4561 acctccgcct gaagcggact ctcatcctct tcaaaatcct ccacca
//
LOCUS       DAOQFT010000135         4601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9915_length_4601_cov_6.832600, whole genome shotgun sequence.
ACCESSION   DAOQFT010000135 DAOQFT010000000
VERSION     DAOQFT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9915_length_4601_cov_6.832600"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1064
                     /locus_tag="PLR93_04755"
     CDS             78..1064
                     /locus_tag="PLR93_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH26305.1"
                     /translation="MQYRHLGRTGIQVSELSIGSWVTFKNQVDVQAAVEIMAAAYDHG
                     VNFYDNAEVYAGGKSEEVMGAALKKLNWRRGSYLVSTKFYWGIEEGVNEKNTLNRKRL
                     IEGINGALKRLDMDYVDLIYCHRPDKTTPIEETVWAMHNIIEWGKALYWGTSEWAASE
                     IIEAIQIAERHHLHKPVVEQPQYNLFERKRLEGDYVRFYKEYGYGTTIWSPLASGLLS
                     GKYNKGIPADSRGALKGYEWLHGSLTNQEKLAKVAGLEPIAKELGGTVAQLAIAWCLK
                     NPFISTVITGASRVEQVHENMKSAEIAAKLTPEVMGKIDVVFGVQKKDEDDD"
     gene            complement(1105..2514)
                     /locus_tag="PLR93_04760"
     CDS             complement(1105..2514)
                     /locus_tag="PLR93_04760"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNH26306.1"
                     /translation="MPEREIYLLSPRALSPETIAVAFAKTSRAPESFREIAAELSDES
                     SAKFHEKWVVGYGHASVAEHAVLHIAFENVSRVAIETIEGNRLASYTEKSTRYQKWGS
                     DDFTIPPELDGHPLRDEFIATVRLLFSTYAESLDPVKNLIVERTPRRENETDEAYDRR
                     IRSQYVDRCRFLLPAAANANVGMTANARVIEMTIRKMLSHPLEEVRQIGEKLKEVSKG
                     ETPTLVKYADANPYTVETIRDLENRPSQIENRKSDWCELIEYDKDGEKKILAAALYRF
                     DEIPYADALAYVKALKKKEREALAETLLGKLGKYDTPLRELEYSNYTFDLVMDQGAYA
                     EFKRHRMMSQTPQRLTTRLGYTTPLLITEAGFGSKYEAAMEAASQMYEKLHAFNPDVA
                     QYIVPNGFNRRVLAQFNLREAFAFCQLRTAANAHFSIRRVAQKIYEDISEVHPLLSKY
                     MKLREETWQSVEENYFSKI"
     gene            complement(2590..2931)
                     /locus_tag="PLR93_04765"
     CDS             complement(2590..2931)
                     /locus_tag="PLR93_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNH26307.1"
                     /translation="MSDCIFCKIIKGEIPSTNVYKDEFVTAFRDINPAAPTHILIVPN
                     KHIDSVNMLIPDDEPLIGKMFSIAKQLANKEGIAESGYRLLVNTGEEAGQTVFHIHLH
                     LLGGRTMRALG"
     gene            complement(2945..4042)
                     /locus_tag="PLR93_04770"
     CDS             complement(2945..4042)
                     /locus_tag="PLR93_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26308.1"
                     /translation="MQSPRNTFIPIFLRLTSIVSLVSILVSCQLSPSITNTAEAPTEI
                     ADQTIPVLPETFQTDLLNPVDVPHTYVEETCRYLRNKWNPANAQPGTVVMLILIKDIN
                     RGAVELPNSIRITDFYDLLEQLKAQGFEAINTEKLQAFLERNLPIPPRSVVLIQDGNH
                     TEEYFDKNYRWYYEQWGWPVVNGFVSDPNIDPILREGNFSLEYEGFIDHQARGITPDV
                     VLTDDIAKTIIARELQGSLEGFAEQLGKTPNAIIWPNGGFGIRPIEAARQLRFKLGVT
                     QNPRGPVMYNWVPLANSVDDARPTLIPEGKVGDPLMTLPAYSPQEAFNAIDSVRNIGN
                     AAQAYAESNKDAEYQYYDIVCSDKYGPIPTP"
     gene            complement(4146..>4601)
                     /locus_tag="PLR93_04775"
     CDS             complement(4146..>4601)
                     /locus_tag="PLR93_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26309.1"
                     /translation="GYIHNIPISDGSTDDFITSEMGGVITTFQKYFNKTPIAYIWPGG
                     GFSKRAAEIGVQLGYKLGFTVNPRGPVMYNWIPQADAYNEDNPYAIPETPAGNPLMTL
                     PRYMDITAGDHIDEVRVMSQEAAAYAEQNKATELEYYNIVCAPTYGPIP"
BASE COUNT         1155 a          951 c         1204 g         1291 t
ORIGIN      
        1 caaggttttt cgcatgcagt gattataatg tgctcgaaaa atacggcgga ttcgccataa
       61 aatcttttgg aggaacaatg caatatcgtc atcttggaag aacgggcatt caagtcagtg
      121 agttatctat tgggtcttgg gttacattca agaatcaagt ggatgtgcaa gccgcagtgg
      181 agatcatggc agcggcgtac gatcacggcg tgaactttta cgataacgcc gaagtgtatg
      241 caggcggaaa atcggaagag gtgatgggcg ctgcattgaa gaagttgaac tggcgccgtg
      301 gtagttattt ggtttccacc aagttttatt ggggcattga agaaggggtt aacgaaaaga
      361 ataccctcaa tcgcaagcgt ctcattgaag gtatcaacgg tgcactcaaa cgtctcgaca
      421 tggactatgt tgacttgatc tactgtcacc gcccagataa gaccacgccc attgaagaaa
      481 cagtttgggc gatgcataat attattgaat ggggcaaagc cttgtattgg ggcacttctg
      541 aatgggcggc ttctgagatt attgaagcca ttcaaattgc agaacgccat cacttgcata
      601 aaccggtagt tgaacagccg caatacaacc tttttgaacg caaacgcctc gaaggcgatt
      661 atgtccgctt ctataaagaa tatggctatg gcactaccat ttggagcccg ctcgcttctg
      721 gtttgctgtc tggtaaatat aacaagggca tccctgctga tagccgcggc gcactcaaag
      781 gttatgaatg gttgcatggc agcctcacca atcaagaaaa actggcgaag gttgcaggtt
      841 tggagcctat tgccaaagaa cttggcggca ccgtggcgca actggcaatt gcatggtgct
      901 tgaagaatcc cttcattagc acggtcatta ccggtgcaag ccgtgtggaa caagttcatg
      961 aaaacatgaa gtcagcagag attgctgcca aactcacgcc cgaggttatg ggtaagatcg
     1021 atgtggtctt tggtgtgcaa aagaaagacg aagacgacga ttaaataaaa aaactccgag
     1081 gtttcaagac ctcggagttt ttgtttagat cttcgaaaaa taattttctt ccacgctttg
     1141 ccaagtctct tcacgcagtt tcatatattt tgaaagcagt ggatgaactt cggaaatgtc
     1201 ttcgtaaatt ttttgtgcca cgcgccgaat tgaaaagtgg gcattggcag ccgtgcgcaa
     1261 ctgacagaag gcgaaggctt cccgtaaatt gaattgcgcc aacacccggc gattaaatcc
     1321 gttcgggacg atgtattggg caacgtctgg attgaacgca tggagctttt catacatctg
     1381 tgatgccgct tccatcgccg cctcatactt ggacccgaaa cctgcttccg ttataagtag
     1441 cggagtcgta tacccgagcc ttgtggtcag ccgttgtggg gtctggctca tcatacggtg
     1501 acgtttgaac tcggcatatg cgccttggtc catgacaagg tcgaaagtgt agttggaata
     1561 ttccaattca cgcagagggg tatcatattt gccgagtttg ccaagcaagg tttcagcgag
     1621 tgcttcacgc tcttttttct taagcgcttt gacgtatgcc agagcatcgg cataggggat
     1681 ttcatcaaag cggtataacg ccgcagcgag aattttcttt tccccatctt tgtcgtactc
     1741 aataagttcg caccaatctg atttccgatt ttcgatttga gatggacgat tttccaaatc
     1801 acgaatggtt tcgactgtgt atggattagc gtctgcatat ttgactaagg tgggtgtttc
     1861 gcctttagag acttctttta acttctcgcc aatttgacgt acttcttcga gtgggtgaga
     1921 aagcattttg cgaatggtca tttcgatgac gcgcgcattg gcggtcatac ctacattggc
     1981 attggcggcg gcgggcagca gaaagcggca acgatccaca tattgcgagc ggatgcggcg
     2041 atcgtaggct tcatctgttt cgttttcacg gcggggagtc cgctcgacga tgagattctt
     2101 gaccgggtcc aatgactcag cataggtcga gaaaaggagg cggacggttg ctatgaattc
     2161 atcgcgcaga ggatgtccgt ctaattctgg cggaatggtg aagtcatcgg agccccattt
     2221 ttggtagcga gtggactttt ccgtataaga agcaaggcga ttgccttcga ttgtttcaat
     2281 ggcaacacgt gagacatttt caaacgcgat atgcaggaca gcatgttcgg ctacagaggc
     2341 atgaccataa ccaacgaccc atttctcatg gaactttgct gaagattcat cgctaagttc
     2401 tgcggcgatt tcgcggaaag attcaggggc gcgggatgtt tttgcaaatg ctacggcaat
     2461 agtctctggg ctgagggcgc gtggggataa aaggtatatc tctcgttcgg gcatggtgtc
     2521 tcctgtatgt gttgaatcta tttcataaaa aagcgaccag cccgttgtgc gctgatcgct
     2581 tcgatgattt taccctaagg ctcgcattgt cctgccgccg agcaggtgca ggtgaatgtg
     2641 gaagacggtc tgcccggctt cttctcctgt gttgactaga agacgatagc cactttcagc
     2701 aataccttct ttgtttgcta gttgtttggc aatggaaaac attttcccga tcaacggttc
     2761 gtcatcagga atgagcatgt tgacggaatc aatgtgtttg ttcggtacga tcaagatatg
     2821 agttggtgcg gcagggttga tatcgcggaa ggcggttacg aattcgtctt tatatacatt
     2881 cgtgctggga atttcacctt tgatgatttt gcaaaaaata caatcagaca tagtagcctc
     2941 ctatttatgg ggtcgggatg ggaccatatt tatcggaaca aacaatatca taatattggt
     3001 attcggcatc tttgtttgat tctgcatatg cctgagccgc attcccgata ttacggacac
     3061 tgtcaatagc attgaatgct tcttgtggtg agtaagcggg taaggtcatg agtgggtcac
     3121 caactttgcc ttcagggatt aaggttggtc gtgcatcgtc gacgctgttt gcgagcggca
     3181 cccaattgta catgacgggt ccgcgtgggt tttgagtgac tcccagcttg aagcgtaact
     3241 gtcgcgctgc ttctatggga cgaattccaa acccgccgtt gggccagata atggcatttg
     3301 gtgttttgcc taattgttct gcaaaccctt ctaaagagcc ttgcaactca cgggcaataa
     3361 ttgttttggc aatgtcatca gtgagaacta catcgggtgt gatgccgcgc gcttggtggt
     3421 ctatgaagcc ttcatattca agcgagaaat tcccttcgcg taagataggg tctatattag
     3481 ggtcactgac gaacccattg acgacgggcc agccccactg ttcataatac cagcgatagt
     3541 ttttatcgaa atattcttct gtgtgattcc catcttgaat taatacaaca gaccgcggag
     3601 ggatgggaag gttccgttcc agaaatgctt gtagtttttc tgtattgatc gcttcgaacc
     3661 cctgtgcttt taattgttct aataagtcat agaaatctgt gatgcgaata ctatttggaa
     3721 gttctacggc gccacgattt atatccttga taaggatgag catgacaacg gtgcccggtt
     3781 gcgcatttgc agggttccac ttgttgcgta aataacgaca ggtctcttct acataggtat
     3841 gtggaacatc aacggggttt aataaatcgg tttgaaacgt ttctggtaaa acagggatgg
     3901 tttgatctgc tatttcagta ggagcttctg ctgtatttgt gatagatgga cttaactggc
     3961 aggaaaccag aatggaaacg agtgagacaa tggatgtgag gcgaagaaag atgggaataa
     4021 acgtatttct tggcgattgc atataaatag aattgtaaac cagtttttga gaatgagttt
     4081 tggcttatgt aaacaggtca ggcattgcct gacctgttta ggttgtactt aaaaagaatt
     4141 attaactagg ggataggtcc gtatgttgga gcgcagacga tgttgtagta ttccaattcg
     4201 gtggctttgt tttgctcggc gtaagcagcc gcttcttggc tcatgacacg aacttcgtcg
     4261 atatgatctc ctgcagtgat gtccatgtag cgtgggaggg tcattaaggg attcccagct
     4321 ggtgtttcag gaatggcata tggattatct tcattgtagg catcggcttg aggaatccag
     4381 ttatacataa cgggtccgcg tgggttgacg gtaaagccga gcttataccc aagttgaaca
     4441 ccgatctcag cggctcgctt tgaaaacccg ccgccgggcc agatataagc aatgggggtc
     4501 ttgttgaaat atttttggaa ggttgtaatc acacccccca tttctgaggt gatgaagtca
     4561 tctgtagaac catcactaat ggggatgtta tggatgtagc c
//
LOCUS       DAOQFT010000136         4599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9924_length_4599_cov_6.984375, whole genome shotgun sequence.
ACCESSION   DAOQFT010000136 DAOQFT010000000
VERSION     DAOQFT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9924_length_4599_cov_6.984375"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1675)
                     /locus_tag="PLR93_04780"
     CDS             complement(224..1675)
                     /locus_tag="PLR93_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNH26310.1"
                     /translation="MSHYLFLLIAFALNFVASGGKWNVPIAAWIAPIFVLRFYRQSEK
                     PWLDFLLLWLATAIPLIVSWKGATFFPGLGEIGFFLAVAPIALIAVVTDRYFHVRFPD
                     SAWMLFIFPIAATALDFFQANGSPFGTFGASAYSQRGFLPVMQIASVTGIWGITFAMS
                     LFASTVNRFWEGNASPLSWASAGLLVLILGLSLGRTLLPTQPKQTAVIAGFSLPEGTL
                     SKSLSQFKSGDEAGFRQAMDELHAAELNQIRTLAQDGANIVVIQEGGVIGMTDQIEKM
                     IADAGTLAKEENIYVVLPTFDIEQTPPVNSIYIVDPNGDVVLHHIKYGGNMFEGTLKG
                     DGVLQTVDTPYGKLSAIICWDADFPNIVKQAGSQNVDLLIVPAQDWLGVRDIHAGMAT
                     FRAVENGLTVFRQTGQGVSLVSDPYGKVLNLVDSFEDTEGKFVSIQKVSAPIGSLNTL
                     YPKLGDALGNAMLLGLVGSLVGLWKTRKRPTRL"
     gene            1857..3062
                     /locus_tag="PLR93_04785"
     CDS             1857..3062
                     /locus_tag="PLR93_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNH26311.1"
                     /translation="MDTANLNLDRKMVFPFYIFLTLLMGYMGTASVMSVWEQGEMVVI
                     GSVIALFVMHIGLYWLNVLWVTRNDRWFLFYYPAQTVVMLGIVWLLHGRSIVGLTFLF
                     SATIAMVGEGLGVWGNSRRAFFIGIFYVGLLLGFLYLYSDRATLMDSVTGLLVNGGSI
                     ILMMMMFNQLFVQRQKAIDLAESLESANAKLAASAAKIESLTLQNERQRMARELHDTL
                     AQGVAGLVLQLEAIKAHLGSNRTERASAIVEQSITRARSTLAESRAAIDNLRAVPVNV
                     ADAVREQVEHFKQSTGVACDLDISVTENQLSPEVVDHAMNILSESLTNISRHAQASKV
                     KVEFFIQNKTLELDVRDNGKGFDVKQETKGHYGLVGMHERARLTGGMLSIESDSNGTC
                     VQFIVGGEA"
     assembly_gap    3094..3193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3194..3573
                     /locus_tag="PLR93_04790"
     CDS             <3194..3573
                     /locus_tag="PLR93_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26312.1"
                     /translation="MIRGLQSGARGYLLKDSSRESLLDAIHAAAKGETLLKPEILARV
                     LTSSSRGKPESTPQTDTTLTERELEVLQLAAKGERNKEMAYKLGITERTVKAHLQSIY
                     QKFGVDSRAAAVAVAAGKGLLEN"
     gene            complement(3988..4110)
                     /locus_tag="PLR93_04795"
     CDS             complement(3988..4110)
                     /locus_tag="PLR93_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26313.1"
                     /translation="MLHIVYRAILQGGFEFIEELRHAAWDCQCQNFEAFFLRIV"
     gene            complement(4163..4465)
                     /locus_tag="PLR93_04800"
     CDS             complement(4163..4465)
                     /locus_tag="PLR93_04800"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNH26314.1"
                     /translation="MILEVVILNIIPGQEEEFEAAFAKASPIISSMDGYHWHQLQHCL
                     EVENRYLLLVQWETLEAHTVGFRGSAEYQEWRKLLHPFYDPFPTVEHYEVVQDYKI"
BASE COUNT         1101 a         1081 c         1228 g         1089 t
ORIGIN      
        1 gcgctggagt catcagcggc ttcgacatga ccgccgaagc cgcgctggca aaactatcct
       61 atctattcag tcaaaatctg cccgtgaatg aaatcaaacg cttgatgcaa actaatctac
      121 gcggggaact cacggcataa gttttcctat ttcaattgac aaatggtgtg ccatatcctt
      181 gctgcaccat aaatccaagt cagtggaaat attttttaca aggtcatagc cttgttgggc
      241 gttttctcgt cttccacaac ccaactagtg aacccaccaa gccgagcagc attgcatttc
      301 caagcgcatc gccgagtttg ggatagagtg tgttcaaaga gccaataggc gcggatactt
      361 tttgtatgct gacgaacttg ccttctgtgt cttcaaatga gtctacaagg ttcaaaactt
      421 ttccatacgg gtcgctgacc agtgatacgc cctgacccgt ttggcggaag accgtcagtc
      481 cattttccac ggcgcggaag gttgccatgc ccgcgtggat gtcgcgtacg ccgagccagt
      541 cttgcgcggg gacgatgagc aagtccacgt tttgtgagcc agcttgtttg acgatgttcg
      601 ggaagtcggc gtcccagcag atgatggcgc tgagtttgcc gtagggcgta tccacagttt
      661 gcagaacgcc atcacctttg agtgtgcctt cgaacatgtt gccgccgtac ttgatgtgat
      721 gcagaaccac atcaccattt gggtcaacga tataaatgga gttcacgggc ggagtttgtt
      781 cgatgtcaaa ggtcggcagg acaacataaa tgttttcttc tttggcaagc gtgcccgcat
      841 cggcgatcat tttttcgatc tggtcggtca tgccgatgac accaccttct tgaatgacga
      901 cgatgtttgc gccatcttga gcgagcgtgc ggatctgatt caactcggcg gcgtgcaatt
      961 catccatggc ttgacgaaaa cccgcttcgt ctccactttt gaattgactc agcgacttgg
     1021 agagagtccc ctctgggagc gagaagcctg cgatgacggc ggtctgcttg ggttgagtcg
     1081 ggaggagagt cctgccgagg ctgagaccaa gaatgaggac gagcaaccca gccgaagccc
     1141 aggaaagagg cgaagcattt ccttcccaaa aacgattgac cgtgctggcg aagagactca
     1201 tcgcgaaggt gattccccaa atccctgtga cggatgcgat ctgcatgacg gggagaaatc
     1261 cgcgttgcga gtaggcggat gcgccgaatg taccgaaggg actgccgttg gcttggaaga
     1321 agtccagcgc ggtggcggcg atggggaaga tgaagagcat ccatgccgag tcggggaagc
     1381 ggacgtggaa gtagcggtcg gtgacgacag cgatcaaggc gatgggcgcg acggcgagga
     1441 agaagccaat ctcacccaga cctgggaaga atgtggcgcc tttccatgaa acgataagcg
     1501 gaatggcggt agcaagccag agcaagagga agtcgagcca gggcttttcg ctctgacgat
     1561 agaagcgcag gacgaagatg ggcgcgatcc acgcggcgat ggggacgttc catttgccgc
     1621 ctgaggcgac gaagttgagt gcgaaggcga tgagtaggaa taagtagtgg gacataggag
     1681 ggctcctttt tggtgattgg taattcgtaa ttggttgacc aaagagtgag cagtggtcag
     1741 ttgatgttgt cattctacgg tttcaccttg ccttccacat cgtccattcc atcccaaagc
     1801 ggacactgtc cgttcggaca atatgcggct gtgcaggttg aagtacaatg cgaagcatgg
     1861 acacagccaa cctcaacctt gaccgcaaaa tggtcttccc gttctacatt ttcctgaccc
     1921 tgttgatggg ttacatgggc acggcgtcgg tgatgtcggt atgggagcaa ggcgaaatgg
     1981 tcgtgatcgg ttcagtgatt gcgttgtttg tcatgcatat cgggctgtat tggttgaacg
     2041 tgctctgggt gacccgtaac gaccgttggt ttctgttcta ttaccccgca cagacggtcg
     2101 taatgcttgg cattgtctgg ctgctgcacg ggcgctccat agtggggttg accttcttgt
     2161 tttcggcgac cattgcaatg gttggcgagg gactgggcgt atggggtaac tcgcgccgtg
     2221 ctttcttcat tggcattttt tacgttgggc tgttgttagg cttcctttat ttgtattctg
     2281 accgagccac cttgatggac tcggtgacag gtttgctcgt caacggcggt tcgattattt
     2341 tgatgatgat gatgttcaat cagttgttcg ttcaacggca gaaggcgatt gaccttgcgg
     2401 agtctttgga aagtgcgaat gcaaaacttg ccgcttcagc cgccaagatc gagtcactga
     2461 ccttgcagaa tgaacgtcaa cgcatggcac gcgaattgca tgacacactg gcgcaaggcg
     2521 tggctggact cgtcttgcaa ctcgaagcga tcaaagcaca tcttgggtct aatcgaaccg
     2581 agcgtgcttc tgccatcgtg gaacagtcca taacccgcgc acgcagtacg ctggctgagt
     2641 ctcgcgccgc gatagataat ctccgcgctg tgcctgtcaa tgtggcggat gcggtgcgcg
     2701 agcaagtgga gcatttcaaa cagtccacgg gtgtggcgtg tgatttggat atttctgtga
     2761 ccgagaatca actgtctcct gaagtcgttg accatgccat gaacatcttg agcgagtcac
     2821 tcacgaacat ttcccgccat gcgcaggcaa gcaaagtgaa ggtggaattc ttcattcaaa
     2881 acaaaacgct ggagttggat gtccgtgata acggcaaagg gtttgatgtg aagcaggaaa
     2941 caaaaggaca ctacggcttg gtcggaatgc acgaacgcgc gcggctaacg ggaggaatgc
     3001 tttccatcga atcagactca aatgggacat gtgtgcaatt cattgtgggc ggtgaagcat
     3061 gatccgcgtg atgattacgg atgaccatct catnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnntgatgat ccgcggtttg caatctggcg cgcgcggata tttgctcaaa
     3241 gattcaagcc gcgaaagttt attggatgcg atccacgctg cggcgaaagg ggagacgtta
     3301 ttgaagccgg agatccttgc gcgcgtgctt acgtcttctt ctcggggcaa gcccgagtct
     3361 acgccccaaa ccgataccac gctgacagaa cgcgagcttg aagttttaca actcgcggcg
     3421 aaaggcgaac gtaacaaaga aatggcgtac aaactgggaa tcaccgaacg aactgtgaag
     3481 gctcatctgc aaagtatcta tcaaaaattt ggggtggatt cgagagcggc ggcggtcgct
     3541 gtggcggcgg ggaaaggatt gttggagaat taaaagtgac agtcacctta aaggtgactg
     3601 tcacttcttt aaactttact tagcaaaccg aaataataga ggatttgtct tttgtcagtg
     3661 gggggcactg taagcgatga aggctctccc tcatccaata cgagagcgat tgccgcctcc
     3721 atctcgtcat ggcgcgggtt cttacaagaa tttcgtcaat gaatttcctg tgcgtaagtc
     3781 agacaatgga acgaatcttt tggcgcaagt tgtctgtcca gacgattaac atcgcagtgc
     3841 ggataaggtc ttcttgtttt aagaacagac aggcactatt tgggtgtttt tcacaggacg
     3901 cattcataaa gcaattcttt tgtctcctcc atatcaccgt gatgaagaag cgtaatcgcg
     3961 catctactaa aatagattcc gcatgcgcta cacaatccgc agaaagaacg cctcaaaatt
     4021 ttgacactgg caatcccagg cggcatggcg caattcttca atgaactcga atccgccttg
     4081 caggatggca cgatagacga tgtgaagcac aaggaaatct cccaaaagta tggaattgat
     4141 tggaataagt ggaaaattcc aattaaatct tgtagtcctg caccacctca taatgttcca
     4201 cagtggggaa tggatcataa aagggatgta acaatttacg ccattcttga tattccgctg
     4261 aaccgcgaaa cccgaccgta tgggcttcca aagtttccca ttgcaccaat aacaaatatc
     4321 gattctctac ttccaaacaa tgctgcaact gatgccagtg atacccatcc atgctggaaa
     4381 taataggcga cgcttttgca aacgccgcct caaattcttc ttcttgacca gggataatat
     4441 tcaagatgac cacttcgagg atcatttttt caccccagac aacgcgcctc ggaactccgc
     4501 cgcaaactgc ttcgctgttt caacaggctt ttggctactc ccgatcattt tcacgcacgc
     4561 cgagccgaca atgactccat ccgcgagttg accgacctg
//
LOCUS       DAOQFT010000137         4598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9928_length_4598_cov_5.874972, whole genome shotgun sequence.
ACCESSION   DAOQFT010000137 DAOQFT010000000
VERSION     DAOQFT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9928_length_4598_cov_5.874972"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLR93_04805"
     CDS             complement(<1..1241)
                     /locus_tag="PLR93_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH26315.1"
                     /translation="MRTKIIKISFIGLFLFSTVFSQQPDKVHAQSDKIVFAAIGDYGL
                     AGQPLLDVSNLIKSWNPDFIVTLGDNNYPDGAAWSIDDNIGQYFHEYIFKYKGKYGEG
                     SPTRRFYPSLGNHDWTTGANAYFNFFGYYNPVTHYDFIQGPVHFFILDSDRNEPDGVM
                     SDSAQAKWLKKTMLASTSPFNVVVFHHAPYSSGRHGSTEYMRWPFKDWGADVVLTGHD
                     HVYERLSVNGIPYFVNGIGGAEIYNFDRILPESQVRYNQDFGAMRIEATSLYMKFQMY
                     SRAGVLVDEYTIGGSNPSVSSITRLNAASTNLSGVNFQVNFSEAVTGVDVSDFLLTTN
                     LSGANIASINGSGNVYTVTVNTGAGDGTLRLDLTDNDSILNSISIPLGGSGAANGNFS
                     AGEIYTIDHTSPSVSSIATASL"
     gene            1355..2308
                     /locus_tag="PLR93_04810"
     CDS             1355..2308
                     /locus_tag="PLR93_04810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH26316.1"
                     /translation="MKRVFYFVFALLIVACNLPATENSTSEPVNTATLELPTATPSLA
                     PSATGTLIPPTLTATPVPCDPLAVDYCITSGHFLFQRPIHLPDNETVDLTYLYASTSN
                     GKRDPHHGVEYQNPFGTSVFAAGDGEVVFADADKKTKFSPWNNFYGNVIVIRHADEMY
                     TLYAHLSEILVQVGDQVQAGDEIGKVGQTGGATGSHLHFEVRKGGDYTDYFSTENPEL
                     WTLPPEGTGALSITLKTTEERNYERQLVITRLEEGSQDILFTYYITTYAKGFEHNIED
                     AGLGNLPAGQYKIAFTGPTGFVERLVLIEAGHLTEVVFDLQ"
     gene            complement(2360..2947)
                     /locus_tag="PLR93_04815"
     CDS             complement(2360..2947)
                     /locus_tag="PLR93_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26317.1"
                     /translation="MKKFFANEIFLGTMIAVLSVFTAYVSYEGAMADSKQNEFEIKGM
                     KDLNDGNAEYLRANQDITQDYNYFDNWYINDGTDRQDIADYYKENFSEALQTAFDSEE
                     GVWSEAYYDAMYADAYAFWDSSDANFETGSQYDERGDQLQLVMLVTALGLAFVAWASL
                     LGAESNMRVLFAVFGTIALIAGVLVFFLMVPTVAG"
     gene            complement(3103..>4598)
                     /gene="acs"
                     /locus_tag="PLR93_04820"
     CDS             complement(3103..>4598)
                     /gene="acs"
                     /locus_tag="PLR93_04820"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNH26318.1"
                     /translation="VTIYMGRVPEIVFAMLACAKIGAIHSVVFGGFSVDSLQGRIEDS
                     KSKLVITCDGSYQNGKVVELKAIVDESLKKCPTVENVIVVKRVGNGVTMEAGRDHWYH
                     DLCQLPIANGKCPTEEMDAEDPLFILYTSGSTGKPKAILHTHGGYMVGTYSTLKYAFD
                     VNDMDRWWCTADPGWITGHSYLVYGPMIAGVTSMLFEGGPAFPYPNRWWQVIERYGIT
                     IFYTAPTAIRGLMRFGESWPNKHDLSSLRLLGSVGEPINPEAWKWYHRVIGKEKCPII
                     DTWWQTETGAFQITPTPVVPLKPGSGTRPFFGQKAEIVDEQGNPVPDDTEGYLTLLRP
                     WPSMLRTIYGDDERYVNQYWSKYPGRYTTGDSAKRDTDGYYWIIGRVDDVIKVSGHRL
                     GTAEVESALVSHPAVAEAAAIGLPHDVKGQAIYTFVILRAGFAPSPELSEELRQHVSK
                     HMGPIARPEDVKFLDKLPKTRSGKIMRRVLKARAQGLPEGDISTLEE"
BASE COUNT         1220 a         1083 c         1106 g         1189 t
ORIGIN      
        1 aggcttgctg tggcaatcga actcacagat ggagatgtgt ggtcaatggt atagatttcg
       61 cctgccgaga aatttccgtt cgcagcgcca gagccgccta aaggaatgct aatgctgttt
      121 aagatagagt cgttatcggt taggtcgagc cgtagggttc catccccagc ccctgtgttg
      181 accgttactg tatatacgtt gccacttcca tttatgctgg cgatatttgc tccactgaga
      241 ttggttgtaa gaaggaaatc agaaacatct acgcctgtaa cagcttctga aaaattcacc
      301 tgaaaattaa ctccactcag atttgtggat gctgcattaa gccgcgtgat agatgaaaca
      361 gaaggattac tgccaccaat tgtatattca tcgacaagga cgccagcgcg cgaatacatt
      421 tggaatttca tgtataggct tgtggcttct attcgcatgg ctccgaaatc ttgattgtag
      481 cgaacctggc tttcaggcag aattctgtcg aaattataaa tttcagcgcc gccaatgcca
      541 tttacaaagt atggaatgcc atttacggaa agccgctcgt acacatgatc gtgccctgta
      601 aggacaacgt ctgcgcccca atctttgaac ggccagcgca tgtattcagt ggatccatgc
      661 cgcccggaag aataaggtgc atgatgaaaa accaccacat tgaacggcga tgtggatgcg
      721 agcattgttt ttttcagcca ctttgcctgt gctgagtcag acatgacgcc atcaggttcg
      781 ttacggtcgc tgtctaaaat aaagaagtga acaggacctt gaataaagtc gtagtgtgtg
      841 acggggttgt aatacccaaa gaagttgaag taggcgtttg cgccggttgt ccaatcgtga
      901 ttgccaagcg atggataaaa acgacgagtt ggtgagcctt cgccatattt gcctttgtat
      961 ttgaaaatat attcgtgaaa gtattgcccg atattatcgt caatagacca tgcggcacca
     1021 tcaggataat tgttatcacc caaggtcaca ataaaatctg gattccagct tttgattaag
     1081 ttggaaacat ccaaaagagg ctgcccagcc aaaccataat cgccaatggc agcgaagacg
     1141 attttgtcgc tttgcgcgtg gactttatcg ggctgttgtg aaaagaccgt ggaaaaaagg
     1201 aataaaccga taaatgaaat tttgatgatc ttggttcgca tgtctttctc ctgcctgatg
     1261 aggcgacttc atcattatat ttttcgcttc gaaaatcccc ttggctggta tgaaattaat
     1321 atgcttttat tcctgatgta aaatggaaac cactatgaaa cgagtttttt attttgtttt
     1381 tgctttgtta attgttgctt gtaatttgcc tgctacagaa aattcaacat ctgaacctgt
     1441 caacacagcc acactggaat tgccaacagc aactccctct cttgcgcctt ctgccacagg
     1501 aacgctcatt ccaccgactc ttactgctac tcccgtccca tgcgacccgt tagctgtcga
     1561 ttactgcatc acaagtggac atttcctttt tcagcgccca atccacttgc ctgataatga
     1621 aacagtcgat ttaacttatc tttacgcttc tacatcaaac ggaaaacgtg acccgcatca
     1681 tggtgtggaa tatcaaaacc catttggtac ttctgttttt gcagcgggag atggggaagt
     1741 ggtctttgca gatgccgata aaaagacaaa attcagccca tggaataatt tctatggcaa
     1801 tgttattgtc attcgccatg cagacgaaat gtacacttta tatgctcatt tgtctgagat
     1861 attggttcag gttggggatc aagttcaagc tggagacgaa attgggaaag ttgggcaaac
     1921 aggcggggca accggaagtc atctccattt tgaggtccgc aaaggcggcg actacaccga
     1981 ttatttttcc accgaaaacc cagagctatg gaccctcccg ccagaaggta caggcgctct
     2041 gtccattaca ttgaagacaa cagaagaacg gaattacgaa cgtcaacttg tgattacgcg
     2101 tttggaagag ggcagtcagg atattctctt tacgtactat ataacaacat atgccaaagg
     2161 gtttgaacat aatatagaag atgccggttt aggaaacctt cctgccggtc aatataaaat
     2221 cgcgtttacg ggtccaacag gttttgtaga acgcctcgtc ttgatagaag cggggcacct
     2281 cacagaagta gtttttgacc ttcagtagtt aaaaataaaa agcgcatcca tatggatgcg
     2341 ctttttattt tttgctcaat tatcctgcga cggtaggaac catcaagaag aacacaagaa
     2401 cacctgcgat aagagcgatt gttccgaaca cggcgaacag aacgcgcatg ttgctttcag
     2461 cacccaacaa agaagcccaa gccacaaacg ccaagccgag agcggtcacc agcatcacga
     2521 gctgaagctg atcgccgcgt tcgtcatact gtgaaccggt ctcaaaattt gcatcagagc
     2581 tatcccagaa ggcataggca tcggcgtaca tggcgtcata atatgcttcg ctccacacac
     2641 cttcttcgct atcgaaagcg gtctgaagcg cttcagagaa attctctttg tagtagtcag
     2701 caatatcctg gcggtcggtg ccatcgttga tgtaccagtt gtcaaaatag ttgtaatcct
     2761 gggtaatgtc ctgattggcg cgcaagtatt cggcgttgcc gtcattcaag tctttcatac
     2821 ccttgatctc gaattcattc tgcttcgagt cagccatggc gccttcgtag ctcacatacg
     2881 cagtgaacac cgaaaggaca gcgatcatgg ttcccaaaaa tatttcgttc gcaaaaaact
     2941 ttttcatatt ctctcctttt tgaaaaaaat aatacaagcg ccgcgattat atacggaaaa
     3001 atttatctta aggtttacag ttttgttgta aaaaacagac ctccgaaatt cgaaggtctg
     3061 ttttgtggat aaaattccgg ttcagactca gccgtaacct ggttactctt ccagtgtgct
     3121 gatatctcct tcgggcagac cttgtgcgcg ggcttttagc acgcggcgca tgattttacc
     3181 agaacgtgtc ttgggcagct tatcaaggaa cttgacatct tccgggcggg caattggacc
     3241 catgtgtttg gatacatgtt gacgcagttc ttcagagagt tctggagaag gcgcgaaccc
     3301 ggcacgcaag atgacgaagg tataaattgc ctgaccttta acatcgtgcg gcaaaccgat
     3361 ggcggcagct tctgccacag cagggtgact gaccaacgcc gattcaactt ctgccgttcc
     3421 aaggcggtga cccgagacct tgatgacatc gtccacacgc ccaatgatcc agtagtagcc
     3481 gtctgtatca cgtttggcag agtcgccggt agtgtagcga ccggggtact tgctccaata
     3541 ttgattcaca tagcgctcgt catcgccata aattgtgcgc agcatggaag gccaggggcg
     3601 tagcaaggtg agataccctt cggtatcgtc tggaacaggg ttgccttgtt cgtccacgat
     3661 ctcggctttt tgaccgaaga aaggtcgggt gccagagccg ggtttgagag gcacaacagg
     3721 cgtcggcgta atttggaacg cgccggtctc cgtctgccac cacgtatcga tgatcgggca
     3781 tttctcttta ccaatcacgc ggtgatacca cttccatgct tcggggttga tgggttcacc
     3841 cacggagccg agcaagcgca aggaagaaag gtcgtgcttg ttgggccagg attcgccgaa
     3901 acgcatgagc ccacggatgg cagtaggggc ggtgtaaaag atggtgatgc catagcgttc
     3961 aatgacctgc caccagcgat tcggatacgg gaaagcagga ccgccttcaa aaagcatgga
     4021 ggtcacgccc gcgatcatgg gaccatacac aagatacgag tgacccgtga tccagccggg
     4081 gtcggcggtg caccaccagc gatccatatc gttaacatca aaggcatatt tcaaggtcga
     4141 ataggttcca accatgtagc cgccgtgtgt atgcaaaatg gctttaggtt tgccagtaga
     4201 gccagaggta taaagaatga agagcggatc ttccgcatcc atttcttcgg tggggcattt
     4261 gccattggca atgggaagtt gacacagatc gtgataccag tgatcgcgcc cggcttccat
     4321 cgtcacgccg ttgccaacac gtttgaccac aataacattt tcgacggtgg gacacttctt
     4381 gagtgactca tctacaatgg ctttgagttc aacgactttc ccgttttggt atgaaccatc
     4441 gcaggtaatg actagtttcg atttgctgtc ttcgatgcgt ccttgtaagg agtcaacgga
     4501 gaagccaccg aataccaccg agtggatcgc gccgatcttg gcgcatgcca gcatggcaaa
     4561 aacaatttcg ggaacacgcc ccatataaat agtgacac
//
LOCUS       DAOQFT010000138         4581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_9990_length_4581_cov_5.099646, whole genome shotgun sequence.
ACCESSION   DAOQFT010000138 DAOQFT010000000
VERSION     DAOQFT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9990_length_4581_cov_5.099646"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1540
                     /locus_tag="PLR93_04825"
     CDS             131..1540
                     /locus_tag="PLR93_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26319.1"
                     /translation="MSEIKIGIPLHGKMAARAFHQPSFRQRLAEGGFKPLYFLSPTYF
                     RAFDFDPEQYFELQVEQYDEQYQKHFLLQQLRMLRRFVVVTETTDLRFRELIESKLFD
                     ATLVGMAAQMSFVAALRHIPGMGGVLGWLERNLYVTHAHDEKFNKHQITCILTPGMGN
                     YGFWNENQFAHEAQRLGIPTFTAITNYDNIVNMGYRGFEPVCLGVWSKQMADEAMKLH
                     GYPANKMEITGPVQYDRFMMPLPQTREEFLKSLNLDPSKKTILFAGGVNINHYFDIYR
                     VFVEQRAKVWREPFNFIVRPYPHPKLMGSPAWAVLENLFKQSGAYISNPGSIDASGDR
                     TAEFRVDLAFDEGPDELNYLLRYSDVMVNYFSTISLEAAICDLPTIHIGYDPYTFGLR
                     FSVTTGFLQRQTHNRRPLRLAAARVAKSESELFDALENYLGDRTLDRDARRIYAEAEC
                     GELDGGAGRRLVNMIKNRI"
     gene            1582..3351
                     /locus_tag="PLR93_04830"
     CDS             1582..3351
                     /locus_tag="PLR93_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNH26320.1"
                     /translation="MKSILGVSLGHDTSFAHIVNGKVVAVMEAERYFRQKRYKLHCLT
                     MEPGKHISGYQYVSLDDIELFLGMVAKEWGTSFDALAVQNQGRVEEFKNFQQILEKLG
                     FKFGSVHHVDHHLSHAALAFFSSPFKDALVLSYDGEGNDGQTIIFGAKGNTIEYLHND
                     PVRYGQSYNNMGYIVGIKPEVEGTTSGKVMGLTAYGEPVQDWLPYARDYIKKYVKATP
                     RQIEGLNNYGKAHRINDIGVRDIPDLKKFLVPEDEKNTGLKAAVKKATGIKNTVLRLN
                     GPEDKTAQNFAATVQAAWTESVIDLLKPYFGKYENLCVVGGCALNGVTNYEIQQLGVF
                     KATHFVPNPSDCGLSAGAALYVYWNSGGNGTFDGYGEYFSPYLGSEVFDKNDLPALRS
                     QYPNLDLDSELVATILAKLVHLDYMVGVIRGRYEVGPRALGNRSILCNPLNKDMKDIV
                     NKKVKHREWYRPFAPIAISERAADYFTNTSDIPYMSVICYTKPEYQEVLPSITHVDGS
                     SRLQTVGQDAHPFMHRTLKEFEKLTGMPIMLNTSFNPGGEPILNYYAVGLEMLKSTEL
                     DFVLIENTLFCKPGREDLFRTIQ"
     gene            3323..4054
                     /locus_tag="PLR93_04835"
     CDS             3323..4054
                     /locus_tag="PLR93_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH26321.1"
                     /translation="MKICSVLFSKNIMMMSRISAQKNIWKTDGYITYRRFWLDLYLKE
                     FSHEMRGTVLDLGGKRENKRGSFQPPEHEAQAWWYLNLDWVTKPNMFADVTETPLKNA
                     SIDCILCTEVLEHLKDPQACVDEIHRLLRDDGLVFASTPFFYPVHADPYDFQRFTEDG
                     LRHLFREFKSVEVYRMGGYFGVLGLMCELGIPGEEGKTFISKFTRWALKWLSRWLCSL
                     DLSLYGQENTNRQKFTTGYFVKAIR"
     gene            4056..>4581
                     /locus_tag="PLR93_04840"
     CDS             4056..>4581
                     /locus_tag="PLR93_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26322.1"
                     /translation="MKFRIRETASDLGALIKSSLTSADLSIFHEFEPPPTGGGHQFLR
                     AFWKQAEAKGLRIENNQISRTTRACLLNSFNFRETRLSVTRRTSILYVHRVDGPIDVY
                     RGRNDGVDKGIFSVNQRFAEKTIFQSYYSLEKHRELGMEFKNPVVIMNAADPDIFHTR
                     GRDGFSRNRKIRLIA"
BASE COUNT         1204 a         1025 c         1090 g         1262 t
ORIGIN      
        1 cggcaagcag atgatcaaat tcgccgtcga aggatatccc gcctgtggta cgccacagca
       61 ggcgctggcg ttccatgagt tggatgggtt gtcgctatca aagcgagtaa tgaagtattg
      121 cggttaatga atgtctgaaa taaagatcgg cattcctttg cacggcaaga tggcggcgcg
      181 cgccttccat caaccatcct ttcgccagcg tttggcggag ggtggtttta agccgctgta
      241 ttttctcagc cctacctatt tccgcgcatt tgattttgac ccggaacaat actttgaatt
      301 gcaagttgaa caatacgacg agcaatatca aaaacatttt ttgctgcaac aactgcggat
      361 gttgcgccgc tttgtcgttg tgaccgagac cacggatctg cgttttcgag aattgatcga
      421 aagcaaattg tttgacgcca ccctggtagg tatggcggcg caaatgagct ttgtcgcggc
      481 gctgcgtcat attcccggaa tgggtggggt gttagggtgg ctcgaacgaa acctttatgt
      541 aacccatgca cacgacgaaa aattcaataa gcatcaaata acctgtatct tgacgccggg
      601 aatgggaaat tatggcttct ggaatgaaaa ccagtttgcg catgaggcac agcgcttggg
      661 tattcccacc tttaccgcca tcaccaacta cgacaatatt gtcaacatgg gctatcgtgg
      721 ttttgaacca gtttgtttag gcgtatggag caaacaaatg gcagatgaag ccatgaagtt
      781 gcatggttat ccggcaaaca aaatggagat caccggaccc gtgcaatatg accgtttcat
      841 gatgcctttg cctcagaccc gcgaagaatt tcttaagtcg ctcaaccttg atccatctaa
      901 aaagaccatc ctctttgccg gtggcgtcaa tatcaatcat tacttcgata tttatcgtgt
      961 tttcgtagag cagagggcta aggtctggcg cgaaccgttc aacttcatcg tccgcccgta
     1021 tccgcacccc aagttgatgg gttcccctgc ctgggcggtt ttggaaaatc tctttaaaca
     1081 gtctggtgcg tatatttcca accctggctc catcgatgcc agtggagata gaaccgccga
     1141 gttccgcgtt gacctcgcct tcgatgaagg acccgatgag ttgaactacc tgctccgtta
     1201 cagtgatgta atggtaaatt acttctccac cataagcctt gaagccgcca tttgtgattt
     1261 gcctaccatc catatcggtt acgatccata cacgttcggt ttgcgcttca gcgtcacaac
     1321 cgggtttctt caacgtcaaa cgcataatcg gcgtcccttg agacttgctg cagctcgtgt
     1381 ggcaaaaagt gaatcagaac tctttgatgc gcttgagaat tatcttggtg accgtactct
     1441 tgaccgtgat gcacgtcgaa tttatgctga ggctgaatgt ggagagttgg acggtggggc
     1501 aggcaggcgt ttagtcaata tgattaaaaa cagaatctag tatggttatc atcaaaactc
     1561 aatcatatcc tggaggataa aatgaaaagt attttaggtg taagtttagg acacgatacg
     1621 agctttgcac acattgtgaa tggcaaagtt gttgccgtta tggaggctga acgttatttt
     1681 cgtcagaagc gctataaatt acattgcctg acaatggaac ctggtaaaca tatcagtgga
     1741 tatcaatatg tttctctgga tgatattgaa ctattccttg gtatggtggc aaaagaatgg
     1801 ggtacatcgt ttgatgccct tgccgttcaa aatcagggac gtgtggaaga gttcaagaac
     1861 tttcagcaaa ttcttgaaaa actgggattt aaatttggat ctgttcatca tgtagatcat
     1921 cacttgagtc atgctgcact ggcttttttc tcatctccat tcaaagatgc tcttgtcctt
     1981 agctatgatg gtgaaggcaa tgacgggcaa accattattt ttggcgcaaa aggtaatacg
     2041 atcgaatact tgcacaatga tcctgttcga tatgggcaga gttataacaa tatggggtac
     2101 attgtcggaa ttaaacctga ggtggaagga actacatctg gtaaggtgat gggtctcact
     2161 gcctatggtg aaccagtgca ggattggctg ccctatgccc gagactatat caagaagtat
     2221 gtcaaggcaa ctcctcgaca gatcgaagga ctcaataact acggcaaggc gcatcgcatc
     2281 aatgatattg gcgtccgtga tattcccgac ctcaaaaaat ttcttgttcc agaagatgaa
     2341 aagaataccg gtttgaaggc agcggtcaaa aaagcgacag gtatcaagaa tacggttctg
     2401 cgtctaaatg gaccggaaga taaaactgca caaaattttg ccgccaccgt tcaagcagca
     2461 tggactgagt ctgtgattga cttgctcaaa ccttattttg gaaagtatga aaacctttgt
     2521 gtagtgggtg gatgtgcctt gaatggcgtt actaattacg aaatccaaca actaggggtt
     2581 ttcaaggcga ctcattttgt cccaaaccca tccgactgcg gtttgtctgc aggagctgct
     2641 ctttacgttt attggaactc tggtgggaac ggaacatttg acgggtatgg cgaatatttc
     2701 tccccttact tgggttcaga agtctttgac aagaacgatt tacctgccct gcgttcccaa
     2761 tatccaaatt tagatcttga ttccgaactg gttgcaacaa ttcttgcaaa gctggttcat
     2821 ttagattaca tggtgggggt tatccgcgga cgatatgagg ttggaccgcg tgccttgggc
     2881 aatcgttcca tactgtgtaa cccacttaac aaggatatga aggacatcgt caacaaaaag
     2941 gttaaacacc gcgaatggta tcgccccttt gcaccgattg ctatatcaga gcgtgctgca
     3001 gattacttta ccaacacttc agatatcccc tacatgtctg tgatctgtta taccaaaccc
     3061 gaatatcagg aggtattgcc ttcaattacc catgtggatg gaagctcccg ccttcaaact
     3121 gttgggcagg atgcccatcc tttcatgcat agaacattga aggagttcga aaaacttaca
     3181 ggtatgccca tcatgcttaa tacctcattc aacccgggcg gcgaacccat attgaactac
     3241 tatgcagtag ggcttgaaat gctcaaaagc acagaactcg attttgtttt gatcgaaaac
     3301 accctgttct gcaaaccagg gcgtgaagat ctgttccgta ctattcagta aaaatattat
     3361 gatgatgtcg cgcatctcag ctcagaaaaa tatttggaaa actgatggat acattaccta
     3421 tcggcgtttc tggttggatt tatacctgaa agaattttcg catgagatgc gcggcaccgt
     3481 tttggatctc ggcggcaaac gtgaaaacaa acgcggctcg tttcaaccgc ctgaacatga
     3541 agcccaggca tggtggtacc tcaacctcga ttgggtaacc aaaccgaaca tgtttgcaga
     3601 tgtcacagag accccgctaa aaaatgcaag tatagattgt attctttgta ctgaagtcct
     3661 ggaacatctc aaagacccgc aagcctgtgt ggatgaaatt caccgcctcc ttcgtgacga
     3721 tggcttggtc tttgcttcca cacctttttt ctacccggtt catgccgacc cttacgactt
     3781 ccaacgcttc accgaggatg gcttgcgtca tctttttcgt gaattcaaat cagtcgaggt
     3841 ttatcgcatg ggcggttact ttggcgtgtt ggggttgatg tgtgaattgg gaatcccagg
     3901 cgaagaaggc aagactttca tcagcaaatt cacccgctgg gcgctgaaat ggctttcgcg
     3961 ctggttatgc tcacttgacc tgtctttata cggccaggaa aacacaaacc gtcaaaaatt
     4021 taccaccggc tattttgtga aggctattcg ttaatatgaa gttccgtatc cgtgagacag
     4081 cctctgatct gggtgcgctt ataaaaagtt ctctgacctc ggcagacctt tccatcttcc
     4141 acgaattcga gccgccaccc accgggggcg gacatcagtt tttgcgtgcg ttttggaagc
     4201 aggcagaggc aaaaggtttg cggatcgaga ataatcagat ttcccgaact acgcgagcct
     4261 gtttgttgaa ttcttttaat tttcgtgaaa cgcgtctatc tgtgacacgg cgtacgtcta
     4321 tattgtatgt tcaccgagtg gatggtccca ttgatgttta tcgcgggcgg aatgacggtg
     4381 tggataaagg tattttttca gttaaccaac ggtttgcaga aaaaacgatc tttcagtcgt
     4441 attacagctt agagaaacat cgagagcttg gcatggagtt caaaaatcct gttgtcatca
     4501 tgaatgcggc agaccccgat atattccata cacgtgggcg tgatgggttt tcacgtaatc
     4561 gtaaaatccg gcttatcgcc t
//
LOCUS       DAOQFT010000139         4572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10023_length_4572_cov_7.188842, whole genome shotgun sequence.
ACCESSION   DAOQFT010000139 DAOQFT010000000
VERSION     DAOQFT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10023_length_4572_cov_7.188842"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1105)
                     /gene="arcC"
                     /locus_tag="PLR93_04845"
     CDS             complement(152..1105)
                     /gene="arcC"
                     /locus_tag="PLR93_04845"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNH26323.1"
                     /translation="MTNLAVIAVGGNSLIIDDKKVTVESQYQAAKQTCVHIADMIEQG
                     WDVAIGHGNGPQVGYILRRSEIAAKTAGMHEVPLDVCGADSQGAIGYAIQQNLQNILY
                     QRGIKKKVVTVITQTLVDKTDPAFSKPAKPIGSFMDKEEAERRQQEQGWSVVEDAGRG
                     WRRVVASPLPKEIVELETVQTLLEKGIITITVGGGGIPVVDPGDGNYEGVAAVVDKDY
                     ASSLLARSIGANLFVISTAVEKVAINFGKPEQKWLDQITLAEAKAYLAEGIHFAKGSM
                     APKIQAIIWYLEAMPNGKALITNPENIGRALKGETGTWIVP"
     gene            complement(1169..2149)
                     /locus_tag="PLR93_04850"
     CDS             complement(1169..2149)
                     /locus_tag="PLR93_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNH26324.1"
                     /translation="MQTNFRGRDFIGDLDFSKEEVETVLDVAWDLKRKRALGESHALL
                     RDKTLAMLFFFTSTRTRGSFEAGMAQLGGHAAFIDSETTQISHGDTAKEIGEIFGRYF
                     DGIAIRQCDWNFGNQYINDVAKASRVPILNMQCDVYHPFQCLADLMTIQEKKGRDLKK
                     KKVVVSWAYAASYSKPISVPQSLILQMTRFGCDVVLAHPPEFKLMPEIMQQAKDNAKK
                     YGVGFDVTDNMDEAFKDADVVYPKSWGPMLTTTGKEEGKALIEKYKDWITDERRMKLT
                     KDDSIYMHCLPADRGLEVTDGVIDGPHSVVYDEAENRLHAQKAVMALTMS"
     gene            complement(2196..3170)
                     /locus_tag="PLR93_04855"
     CDS             complement(2196..3170)
                     /locus_tag="PLR93_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH26325.1"
                     /translation="MRKALPVLFILTMLLLACGSNTAKPKVALMLARGGLGDKAFNDS
                     ANEGLQKAAQELNAEVTTFDYAEDRVEANIRQAAEDGYSLIIGLGSENSQAIATVAKE
                     FPETKFAIIDATAEGDNVTSVTFSELEGDFLAGALSALMSENGKVGYLGGADVLVIRR
                     IQYGFEQGVKYINPDAEIVVQYIGGKDDFSGFAKPDEAQTIAAQMYSDGVDIIYAAAG
                     GSTLGAIKAAESAGKPIVTTGSDQRYLAPNVVATSRTKNMNEAVFMLIQKFVDDSLPS
                     GTIQLDYKSGGIGLAPLADFVPASVSEAFLFVKSDIEDGAIKVEPFTE"
     gene            complement(3167..3706)
                     /locus_tag="PLR93_04860"
     CDS             complement(3167..3706)
                     /locus_tag="PLR93_04860"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNH26326.1"
                     /translation="MKILEVRRHSIRHAGGDHLSQAGVALARNVGQNIGPFDFVSTST
                     LPRAFDTAIAMGFAVNEQNPLMNTYGGAVEREAPWPMPFFHYSEIVKQAGAAAKYAHD
                     LMDYYLGILNHIPQGGSALVVNHGGVVELGVVACLPDADFSAWGDAVEYCEGARLFWD
                     GGKFVNGEVLRLMYNKESL"
     gene            complement(3720..>4572)
                     /locus_tag="PLR93_04865"
     CDS             complement(3720..>4572)
                     /locus_tag="PLR93_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNH26327.1"
                     /translation="TPGMIYGLAIARDLGLLDGWTVYYFGNMEEWCDGIAPNTFAEVD
                     PGIKPDFVVIGEPTKMLVYRGHKGRLEMKVTAKGKSAHAASNHLGDNAIYKLLPVIAG
                     IRDLEPKLGDHEFLGHGKITVSDMHVKTPSINAVPDEAVIFIDRRMTFGETKEAVKKQ
                     VEDLIPAEFKDTVKLEELFYDEPSYTGFVFPVDKYFPAWAYEENHPLVQAGQEARKFI
                     GLPEAKPSKWNFSTNGIYWAGKAGIPSIGFGPGDEETAHTVNDSVSLEDMVKATEFYA
                     ILPSLIK"
BASE COUNT         1127 a         1221 c         1066 g         1158 t
ORIGIN      
        1 aaaagagtat atgttagcgc ggcaattaac agcaaaaacc aaaccgtact gcgcggtgta
       61 aattggatgt tgttcaaacg gtcagtcaat tttttcattg gaacaattgt aaacgaatcc
      121 gctgccgaaa ttggcagcgg aattacatcg tctatggaac aatccaagta ccggtttcgc
      181 ctttgagcgc gcgccctata ttttcggggt tggtgattag agctttgccg ttcggcatgg
      241 cttcgagata ccaaataatc gcttggatct ttggcgccat ggagcctttg gcaaaatgga
      301 ttccctcagc caagtacgct ttggcttcgg ctagtgtgat ctggtccagc catttttgct
      361 cgggtttacc aaagttgatg gcaacttttt ccaccgccgt tgaaatgaca aatagattcg
      421 caccaataga gcgggctaac aaagaagagg cgtaatcctt gtcaaccaca gccgccacgc
      481 cttcatagtt tccatcgcca gggtcaacga ccggaatgcc gccgccgcct actgtaatgg
      541 tgatgattcc tttttccaac agggtttgaa cggtttccaa ttccacgatt tcttttggca
      601 aaggcgaggc gacgacccgc ctccaaccac gacctgcatc ttccaccaca gaccagcctt
      661 gttcctgctg acggcgttcc gcttcttctt tatccataaa gctgccaatg ggtttggctg
      721 gctttgaaaa tgccgggtct gttttatcta ccaaggtttg agtgatgacg gtaacaacct
      781 ttttcttgat gccacgttgg tagagaatgt tctgcaaatt ttgttgaatg gcgtacccga
      841 ttgcgccctg gctatcagca ccgcacacat ccaaagggac ttcatgcatg ccagccgttt
      901 tggcagcaat ttcagagcgg cgcaaaatgt agccaacctg tggaccattg ccatgcccaa
      961 tcgccacatc ccagccttgt tcgatcatat cggcaatgtg cacacaggtt tgctttgccg
     1021 cctgatattg gctttctact gttactttct tatcatcaat aatgagcgag ttaccgccca
     1081 ctgcaatcac agcaagattt gtcatctata aagtctcctg agcaaaagac tagcaggggc
     1141 acactggagt gtgcccctgc aaattaactt aagacatcgt caaagccatg accgccttct
     1201 gagcatgcag acggttttca gcttcgtcat ataccaccga gtgcggaccg tcgatcacgc
     1261 catcggtcac ttccaaaccg cggtcagcgg gcagacagtg catatagatg gagtcatcct
     1321 tggtcagctt catgcggcgt tcgtccgtga tccagtcctt gtacttttca atcagtgctt
     1381 tgccttcttc cttacccgtg gtggtcagca taggacccca actcttgggg tagaccacat
     1441 cggcatcctt gaaagcttca tccatgttgt cggtcacatc gaagccaacg ccatacttct
     1501 tggcattgtc tttcgcctgt tgcatgatct caggcataag cttgaactct ggtggatgcg
     1561 ccaacaccac atcgcagcca aagcgggtca tctgaagaat tagagattgt ggcacagaga
     1621 tgggcttgga ataggaagcc gcatacgccc aagaaaccac caccttcttt ttcttgaggt
     1681 cacgtccctt cttttcctga atggtcatca ggtccgcaag gcactggaag ggatgataga
     1741 catcgcattg catattaagg atcggcacac ggctggcttt ggcaacatca ttgatgtatt
     1801 gattgccgaa gttccagtca cattggcgga tggcaatgcc gtcaaaatag cgtccgaaga
     1861 tctcaccgat ttccttggca gtgtcgccat gagatatctg cgtggtttcg ctgtcgataa
     1921 aggcagcgtg accacccaac tgcgccatgc ccgcttcgaa cgacccgcgg gtgcgggtgg
     1981 atgtgaagaa gaacaacata gccagagtct tgtcgcgcag caaggcatga gattcaccca
     2041 aggcgcgttt gcgttttaga tcccacgcca catctaaaac ggtttcgact tcttctttcg
     2101 agaagtcgag gtcgccgata aaatcacgac cacgaaaatt cgtttgcata atttatctcc
     2161 ttatcttttc tagttggtcg ttaaaccaac ctttattatt ctgtaaacgg ctcaaccttg
     2221 atggcaccgt cttcaatatc cgatttcacg aagagaaaag cctcagaaac agaagcaggc
     2281 acaaagtcgg caagaggcgc aagaccaatg ccaccagact tgtaatccag ttggatcgtt
     2341 ccgcttggca ggctgtcatc cacaaatttt tggatcagca taaagacagc ctcgttcatg
     2401 ttcttggtgc gactggtcgc caccacattg ggagctaaat aacgctggtc gcttccggtc
     2461 gtgacgatgg gcttgcctgc gctttcagcc gccttgatcg ccccgagagt ggacccaccc
     2521 gccgccgcat aaataatatc gacaccgtcg ctatacattt gagcggcaat cgtttgagct
     2581 tcatcaggtt tggcgaagcc gctgaaatca tctttgccgc cgatatactg aacaacaatt
     2641 tcagcatcgg ggttgatgta tttcacgcct tgctcaaagc catactgaat acggcggatc
     2701 acaagcacat ctgccccgcc cagataacca accttgccat tctcactcat caaagcagat
     2761 aaagctcctg caagaaaatc gccttcgagt tcactgaatg tgaccgaggt gacgttgtcg
     2821 ccttctgcag ttgcatcgat gatggcgaac ttcgtttcag gaaattcttt ggcaaccgta
     2881 gcaatggctt gtgaattttc agaacccagt ccaatgatga ggctataccc atcttctgcc
     2941 gcttggcgaa tatttgcctc tacacgatct tcggcatagt caaaggttgt gacctctgca
     3001 tttaattctt gagccgcttt ttgcaaacct tcattcgcac tatcgttaaa ggctttgtcg
     3061 cccaagccgc cgcgcgccaa catcagagcc actttgggtt tggctgtgtt gctgccgcat
     3121 gctaaaagca acatggttaa gataaaaaga accggtaaag cttttctcac aaactctcct
     3181 tgttatacat aagccgcaac acttcgccgt tcacaaattt gccgccatcc caaaagagac
     3241 gggcaccttc gcaatactcc acggcatcac cccatgcaga aaaatctgca tcgggcaggc
     3301 atgccacaac acccagttcc accacgccgc cgtggttcac caccaacgcc gaaccgcctt
     3361 gcggaatgtg gttgagaata cccaggtagt aatccatcaa gtcatgtgcg tacttcgcag
     3421 ccgcccctgc ctgcttgacg atctctgaat aatggaagaa cggcatgggc cagggagctt
     3481 ctcgctctac ggcgccaccg tacgtattca tgagcggatt ttgctcattg accgcaaatc
     3541 ccattgcgat ggcggtatca aatgcacgcg gcaatgtgga tgttgacaca aaatcgaagg
     3601 gaccgatgtt ctgaccaaca ttacgagcca gagcaacgcc ggcttgactc aaatggtcgc
     3661 cgccagcatg gcgaatcgaa tgacgtcgta cctctaaaat tttcatagcc gtaaattgtt
     3721 tatttgatca agcttgggag gatggcataa aactctgtcg ccttgaccat atcttccagc
     3781 gacacagaat cattaacggt atgagccgtc tcttcatcac cagggccaaa gccaatcgag
     3841 ggaatgcccg ctttaccagc ccaataaata ccgttcgtag agaagttcca tttgctgggt
     3901 tttgcttcgg gcaaaccgat gaacttgcga gcttcttgcc cagcttgcac cagcggatga
     3961 ttttcttcat acgcccatgc cgggaaatat ttatcaacgg ggaaaacgaa acccgtataa
     4021 gaaggctcgt cgtagaacaa ttcttcgagc ttgacggtgt ctttaaattc agcagggatc
     4081 aaatcttcaa cctgtttctt gaccgcttct ttagtctcac caaaggtcat gcggcggtcg
     4141 atgaagatca ccgcctcatc tgggacagcg ttgatggaag gcgttttaac gtgcatatcg
     4201 ctgaccgtga tcttgccgtg accgagaaat tcatggtcac ccaacttcgg ttctaaatcg
     4261 cgaatgccgg cgatcacagg cagaagttta taaatggcat tatcaccaag gtgattgctt
     4321 gccgcatgcg cagacttgcc tttagcggtc accttcatct ctaaacgacc tttgtgtccg
     4381 cgatagacca gcatcttggt cggttcaccg atgacgacaa aatcgggctt gatgcccgga
     4441 tctacttcgg cgaaagtgtt aggagcgatg ccgtcgcacc attcttccat atttccaaag
     4501 taatagaccg tccagccatc gagcagaccg aggtcacgtg caatggcgag accgtagatc
     4561 atgccggggg tt
//
LOCUS       DAOQFT010000140         4570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10035_length_4570_cov_5.219491, whole genome shotgun sequence.
ACCESSION   DAOQFT010000140 DAOQFT010000000
VERSION     DAOQFT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10035_length_4570_cov_5.219491"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLR93_04870"
     CDS             <1..723
                     /locus_tag="PLR93_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNH26328.1"
                     /translation="PRVHFKEEDLNDLAASIREHGVIQPLIVSPKGDGTFILIAGERR
                     WQASQRAGLTKVPVVTRQANNQELLEIALIENVQRADLNAMEEAEAYHQLAEDFGLSH
                     EMIAKRVGKSRVAVTNTMRLTNLADAVKQALVDNTITEGHARALLGLSTQKAQTSALQ
                     TIINLSLSVRQSEELIRKLTGVKPVKVKKATVSADVLDVEKRLQRSLGTKVALKHGKK
                     GGTVTIYYYSDEELDALIKKMS"
     gene            797..>2055
                     /locus_tag="PLR93_04875"
     CDS             797..>2055
                     /locus_tag="PLR93_04875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNH26329.1"
                     /translation="MKLLYNANIHTLDSANSHASAILIAGGRIIAVGSKDQLESLAHG
                     KVEKQDMKGKTILPGLTDAHIHMQFYSLGLSKVDCETNTKEECLRRVGERARTLKPGE
                     WVLGHGWQQNDWDGNFPKAAELDAVSPNNPVYLTAKSLHAAWANTSALKLANITDETP
                     DPKDGAVQRDANGKATGIMIETAMALVNAAVPEPTLAELEAAFEKAQSVLWKMGITGV
                     HDFDRRQSFMALQSLRAKGKLKLRVCKNIPVESVEQANDLGLRTGFGDEMLWIGSVKA
                     FMDGALGPRTAAMFQPYEGEKENRGILNMDGEELFEHARKAADVGLSMTVHAIGDKAN
                     HEVLNAFEQLRNYETQNHLPHLRHRIEHVQVIHPDDAPRLAKLNVIASMQPIHATSDM
                     FAADRYWGKRAELSYAWRTQLNYGAILA"
     assembly_gap    2056..2155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2263..3285
                     /locus_tag="PLR93_04880"
     CDS             2263..3285
                     /locus_tag="PLR93_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH26330.1"
                     /translation="MESKSPKLSPEMLVPRLGEYLVQKGLISEIDLQRALNHQQDVTY
                     KGGQSLLGQALIELKLIDKDTLDQVITEQIIQLRSALQSANRNLEQRVRERTSDLNEA
                     LNRLSELSQMKANFVSNISHELRTPLTHIKGYIELMLAESLGPISDEQRHALTVSQRS
                     TGKLEDMIEDLIMFSLASRGEMSMKLEGVDIRRLVSLVTKTAMRKAEDRGVKLEVLVK
                     DDTPYVQADGEKVSWVVGQLIDNGIKFTPSGGSVTVAIEEEGKNLVQVIVRDTGVGIP
                     QDRMKEIFEPFHQLDGSSTRRYGGTGLGLSLVRQIVEGHGSLLDVKSTEGKGTTFKFP
                     LLAMRD"
     gene            3289..4476
                     /locus_tag="PLR93_04885"
     CDS             3289..4476
                     /locus_tag="PLR93_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNH26331.1"
                     /translation="MDKETLSDLHRIFQDVQAKPDWRSALDALFISMRRSFVFDNVAV
                     YLSERQTRGLDVVYARAIGRGKTAEADAAWGESVANDVITNEHMVIHGPAKGSNHRDR
                     MSQTFVLGIPLYVGEKLRGALLFVRFGGPEYSDIHIHVASLQAIWAAALIERKELQEA
                     REELDSVQRQMRLQDDFVSTISHELRTPLGFIKGYSTSLLRQDTQWDDATQREFLSII
                     DEETDRLAKLIEDMLESARLQSKTIQFKFSPIRLDAVVRDVAARVKTHHPNLVIEMNM
                     DVLPPVLGDGVRLSQVFENLFSNAIKYAPGSKLIVGGSHQKKKVRISFADEGDGIPEE
                     FLPFLFERFYRVPGERTVTGTGLGLYICKQIVMAHHGNIWVESVLDRGTTFFIELPAD
                     PTL"
BASE COUNT         1141 a          999 c         1221 g         1109 t
ORIGIN      
        1 ccgcgtgtgc attttaaaga ggaagacctg aacgacttgg cggcatccat tcgtgagcat
       61 ggagtcatcc aaccgttgat cgtctctccg aagggagatg gcactttcat ccttattgcg
      121 ggagaacgtc gttggcaggc ttcgcagcgg gctgggttga cgaaagtccc tgtggtaacg
      181 cgccaggcga ataaccagga attacttgaa attgcgttga tcgaaaacgt ccaacgcgca
      241 gacttgaacg ccatggaaga agccgaagcc tatcatcagc ttgcggagga tttcgggctt
      301 tcgcatgaaa tgatcgcaaa acgtgtgggc aaaagccgtg tggcggtgac caatactatg
      361 cgccttacca atctcgcaga cgcggtcaaa caagccttgg tggacaatac cattaccgaa
      421 ggtcacgctc gtgctttatt ggggctgtcc actcagaagg cgcaaacctc tgcactgcaa
      481 accattatca atttgtcgtt gagcgtccgt cagtctgaag aattgatccg taagttgacg
      541 ggcgtaaaac ctgtgaaagt gaagaaggca acagtcagcg ccgatgtgtt ggatgtagag
      601 aaacgcctcc agcgtagttt gggcacgaag gttgcgctca aacatggcaa aaagggcggc
      661 acggtgacga tctattatta ttccgatgaa gagttggatg cattgattaa gaagatgtcg
      721 taattcgtag aggcgcggtt tccgcgcctt attcctttgt aaagctttgg gcaggagacc
      781 ctgcacctac aaaaatatga aacttctcta taacgcaaac attcataccc ttgattctgc
      841 taattcgcat gcttctgcga ttctcatcgc aggtgggcgg attattgctg taggtagcaa
      901 agatcagctt gaaagccttg cacatggcaa ggtggaaaag caggatatga aaggcaagac
      961 tatcttgccg ggtctgaccg acgcgcacat tcatatgcag ttttattcgc ttgggctttc
     1021 caaagtggat tgcgagacca atacgaagga agaatgtcta cgccgggtgg gggaacgtgc
     1081 gcggactctt aagccgggcg agtgggtgct gggtcacggc tggcaacaga atgattggga
     1141 tggcaacttc ccaaaagcgg cggagctaga tgcggtctcg ccgaataatc ctgtctatct
     1201 caccgcaaag tcattgcatg cggcatgggc gaatacgtcc gcattgaagt tggcgaatat
     1261 cacggatgaa acgcccgatc cgaaagatgg agccgttcag cgcgatgcga acgggaaagc
     1321 tacgggcatc atgattgaaa cggcgatggc gttggtcaat gctgcggtgc cagaaccaac
     1381 tctggctgag ttggaagcgg cgtttgaaaa agcgcagtcc gttttatgga agatgggtat
     1441 cacgggcgtc cacgattttg atcgccgtca atcgtttatg gctttgcaat ctttgcgagc
     1501 aaaaggcaaa ttaaaattac gcgtgtgtaa aaatatcccc gttgaaagcg tggagcaggc
     1561 gaacgacctc ggcttgcgca ccggcttcgg tgatgagatg ttgtggattg gctcggtcaa
     1621 agcctttatg gatggcgcgc tcggtccgcg cacggcggcg atgttccagc cctatgaagg
     1681 cgagaaagaa aatcgcggca ttctgaacat ggatggcgaa gaacttttcg agcatgctcg
     1741 caaagccgcc gatgttggtt tgagtatgac tgtgcatgct attggcgata aagccaatca
     1801 tgaagtgttg aatgcctttg agcagttgcg caattacgaa acgcaaaatc atttgccgca
     1861 tctgcgtcac cgcatcgaac atgtgcaagt catccatccc gacgatgcgc cgcgccttgc
     1921 gaagctcaac gtcatcgcat ccatgcagcc cattcacgcc acctccgata tgtttgccgc
     1981 cgatcgttat tggggcaaac gcgccgaact ttcttacgcc tggcggactc aactcaacta
     2041 tggcgcgatc ctcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccct
     2161 caaccctgtt gacaatgtcg ccttctgctg tcatgataga tggggagtgg gtcgcttaga
     2221 gatttggcac gaaaaaaaat aaagaaattc ggacggtttc ggatggaatc aaagtcgcca
     2281 aaattatcac cggagatgct cgttccgcgt ttgggggagt atctcgtcca aaaagggttg
     2341 atcagcgaaa tagaccttca acgtgcgttg aaccatcagc aggatgtaac ctataaaggc
     2401 gggcaatctc ttttgggtca ggcattgatc gaactcaaac tcatcgataa agacacactg
     2461 gatcaggtca ttacggagca gatcattcaa ttgcgctctg ccctacaatc ggcaaaccgc
     2521 aacttggagc agcgcgtccg tgaacgcacc tcagacctga atgaggcatt aaatcgcctg
     2581 tctgagctta gccagatgaa ggcaaatttt gtttcaaaca tctcgcatga attaagaact
     2641 ccgcttacgc acatcaaagg ctatattgaa ttgatgttgg cagagtcgct gggacccatc
     2701 tccgatgaac agcgccatgc attgacggtc agtcagcgtt ctacggggaa actggaagac
     2761 atgatcgaag atctcatcat gttctctttg gcatctcgtg gcgaaatgag tatgaaactt
     2821 gaaggcgtgg atattcgccg gctagtttca ttggttacaa agaccgccat gcgcaaagca
     2881 gaagaccgtg gtgtgaagtt ggaagtattg gttaaggatg atacgccata tgttcaagca
     2941 gatggcgaaa aggtcagctg ggtagtaggg caattaatcg ataacggaat caagttcacc
     3001 ccgtcgggcg gaagcgtaac agtcgctatt gaagaagaag gtaaaaatct agttcaggtc
     3061 attgttcgcg atacaggggt cggtattcca caggaccgta tgaaagagat cttcgagccg
     3121 ttccatcaat tggatggctc ttcgacgcga cgctacggcg gcacaggatt ggggttatca
     3181 ctggtacgtc aaattgtgga agggcatggt tctttgctgg acgtgaaatc aacggagggc
     3241 aaggggacga cgtttaaatt ccctctgctt gccatgcgag attaacctat ggataaagag
     3301 acgctttctg accttcatcg aatctttcaa gatgttcaag caaaaccaga ctggcgttcg
     3361 gcactggacg cgttgtttat ctccatgcgt cgttcttttg tgtttgataa tgttgccgtc
     3421 tatttaagtg aacgccaaac aagagggtta gatgttgtgt atgcgcgtgc cattgggcgt
     3481 ggtaaaaccg cagaagcaga cgccgcttgg ggcgaaagcg ttgcaaatga cgttatcacg
     3541 aatgagcata tggtcattca tggacccgca aaaggttcga accatcgtga ccgcatgtct
     3601 caaacctttg tgttggggat tcctttatat gtgggcgaaa aattaagagg cgctcttttg
     3661 tttgtgcgct ttggtggacc tgaatattct gatatccata ttcatgtcgc ttcgctacag
     3721 gcgatttggg cggcggcgtt aattgagcga aaagaattgc aagaggcgcg cgaagaactg
     3781 gactctgttc agcgccagat gcgtttgcaa gatgattttg tctctacgat ttcgcacgag
     3841 ttacgtactc cgcttgggtt tattaagggg tatagcacta gcttgttacg gcaagacacc
     3901 caatgggacg acgccactca acgtgagttc ttgtcgatca ttgatgaaga gacggaccgt
     3961 ctggcaaaac tgatcgaaga tatgcttgag tccgctcgct tgcagagtaa gacgatccaa
     4021 tttaagtttt ctcctattcg cttggatgct gttgtccgcg atgtggcagc gcgagtcaag
     4081 acgcaccacc cgaatttagt gattgaaatg aacatggatg tcctgccgcc agtccttggc
     4141 gatggagtac gtctttcgca ggtgtttgag aatcttttta gtaatgccat taagtatgcg
     4201 cccggctcaa agctcattgt tggtggaagc catcaaaaga agaaggtgcg gatctcattt
     4261 gctgatgaag gcgatgggat tccagaggaa ttcctaccat tcttgtttga gcgattttat
     4321 cgtgtgcccg gtgaacgcac ggtgaccggg acgggtttag ggctgtatat ttgcaagcaa
     4381 attgttatgg cacatcatgg taatatttgg gtagagtcag tgttggatcg aggaactaca
     4441 tttttcattg agcttcctgc cgacccaacg ctttgacatt taagtgaaac acgattcttc
     4501 aaggagttct tatggcaaag cgtattctga tcgtagatga tgaaccccgc tatcttcgtc
     4561 tgttagaggc
//
LOCUS       DAOQFT010000141         4542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10119_length_4542_cov_5.461778, whole genome shotgun sequence.
ACCESSION   DAOQFT010000141 DAOQFT010000000
VERSION     DAOQFT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10119_length_4542_cov_5.461778"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLR93_04890"
     CDS             <1..296
                     /locus_tag="PLR93_04890"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH26332.1"
                     /translation="QQGLPRQKKSEAQGTVNMLGLAKTELDRGDIPEALMHYGKLIKK
                     GKHLEEVIGNLTESLYRYPVEVGIWQALGDAYMRANRLKEALEAYNKAEELIR"
     gene            372..824
                     /gene="ndk"
                     /locus_tag="PLR93_04895"
     CDS             372..824
                     /gene="ndk"
                     /locus_tag="PLR93_04895"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNH26333.1"
                     /translation="MEKSLVLVKPDGVQRGLIGEAISRFERRGLRLIGAKFIWVSKEL
                     AEAHYAEHKGKGFYDGLISYITSAPVMAMVWEGPNAVAAIRQTVGSTKPWEAAPGTIR
                     HDFAIEVGRNLIHASDKPETGDRETALWFKKEELVEWSRAIDAWVFEK"
     gene            complement(869..1186)
                     /gene="rsfS"
                     /locus_tag="PLR93_04900"
     CDS             complement(869..1186)
                     /gene="rsfS"
                     /locus_tag="PLR93_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNH26334.1"
                     /translation="MVDALEDKKGEDIVLLDIKEIASFTDYFVICTGTSDRMLDALAN
                     SAIDEVRKKHKKKAKKQGNSRDGWVVVDFGDVVLHLLSPDQREYYQIEDLWEAGKILL
                     RLK"
     gene            complement(1217..1795)
                     /gene="thpR"
                     /locus_tag="PLR93_04905"
     CDS             complement(1217..1795)
                     /gene="thpR"
                     /locus_tag="PLR93_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNH26335.1"
                     /translation="MNQIRTFIALDFPLSILESIEQQTQRLRQTLGEDFIRWVPTHNL
                     HLTLKFLGNIPISHLEFIKQMLTQAADSSSQFDLQISGFGSFPNSKRPRVLWVGVHIP
                     ATLATLQKAVEEGAHHLGYEKEERPFSPHLTLGRVRQGINVTELQKISNTISSFQLGK
                     IGTARVDSVHLYKSDLNSEGSVYTKLFSTPLR"
     gene            complement(1798..2181)
                     /locus_tag="PLR93_04910"
     CDS             complement(1798..2181)
                     /locus_tag="PLR93_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26336.1"
                     /translation="MNSRQKTVMIIEDEADAAELFSEMMRINGFRVIKMFSSAPAIPI
                     ISQEKPDIILLDVMMPDISGLEVLRYIRREPELASILVIILSAKSMPGDIKTGLEAGA
                     SMYLTKPVGFQDLKNAVEKVLQASS"
     gene            2311..2817
                     /locus_tag="PLR93_04915"
     CDS             2311..2817
                     /locus_tag="PLR93_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNH26337.1"
                     /translation="MSELELFRAEKNEFFGSHPQSPLTREQKKDFHGLNYFPENETLR
                     LEVKVEEFSVKDELVMQTTTGGVQHYERFGKFSFDVESQTVELTVYRSEHGFFLPFVD
                     ALAGSETYPAGRYLEPDPLPGGRFFIDFNLAYNPYCAYNEMWSCPITPAENRLKVPIR
                     AGEKLFHE"
     gene            complement(2848..3144)
                     /locus_tag="PLR93_04920"
     CDS             complement(2848..3144)
                     /locus_tag="PLR93_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26338.1"
                     /translation="MNNEHLPTPQNKPSQPEITDEVALRFLKILEQVREEDLSCSDMY
                     ARLDEFVEAEIKDGKDAAILTPLIHEHLDMCSGCCDEYEALLNVVENTSNTQDE"
     gene            complement(3212..3850)
                     /locus_tag="PLR93_04925"
     CDS             complement(3212..3850)
                     /locus_tag="PLR93_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH26339.1"
                     /translation="MSSRTNETWLSDLRSSGPVHEEALSDLRAIIQKGLPYALSRWLS
                     SDDPLFPPLVEEVTQETLLRVLDQLDTFEGRSLFTTWVHKIAIRIALTELRRKRWRDA
                     SLDELTENEDAPPPPGLLADPHASPETSAERKDMVSRVRRILEEELTPKQREALVLLG
                     LQDMPMEDAAKRMKTNRNALYKLLHDARLRLKKRLALEELTPQDVLLAFEQK"
     gene            complement(3968..4041)
                     /locus_tag="PLR93_04930"
     tRNA            complement(3968..4041)
                     /locus_tag="PLR93_04930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4005..4007),aa:Val,seq:cac)
     gene            complement(4084..>4542)
                     /locus_tag="PLR93_04935"
     CDS             complement(4084..>4542)
                     /locus_tag="PLR93_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNH26340.1"
                     /translation="NANIRSYYIRPPYVLPWTTPGGAAVLLPQDSLSSMLVEATTLST
                     YAATRETIKVDVQNGSYFDTMETLASSRLNYAGYETTTSPADNRNYGSTVLVDYTVAQ
                     DPSQRQNIIDVLGTYSANVLSMPDPNSTVQYRVIIGADYEACFRPEDLAH"
BASE COUNT         1151 a          938 c         1207 g         1246 t
ORIGIN      
        1 aacaacaagg gttgccgcgt cagaaaaagt ctgaggcgca gggtacggtc aatatgttgg
       61 gacttgcaaa gactgaactg gaccgtggtg acatccctga ggcgctgatg cattatggga
      121 agttgatcaa gaagggcaaa caccttgaag aagtgattgg taacctgact gagtctttgt
      181 atcgataccc ggttgaagta ggtatctggc aggctctagg tgatgcctac atgcgggcaa
      241 atcgattgaa agaagctctc gaagcctata acaaagcgga agagctcata cggtaacatt
      301 gcgaacgccg ctggaaacag cggcgtttga tagcactagc ttggcaatga aataattttt
      361 ggaggaactc tgtggaaaaa tctctcgtac tcgtgaaacc ggatggtgtt cagcgcggtt
      421 taatcggtga agcaatctct cgcttcgaac gccgcggtct gcgcctgatc ggtgctaaat
      481 ttatttgggt tagcaaggaa ttggcagaag ctcattatgc cgaacataaa ggtaagggat
      541 tttatgatgg gctgatttcc tatatcacct ctgcgccggt gatggcgatg gtgtgggaag
      601 gtcctaacgc tgtagctgct attcgtcaga ctgtgggctc aaccaagcct tgggaagccg
      661 cgcctggcac cattcgccac gatttcgcta ttgaagtagg acgcaatctt attcacgctt
      721 cagataagcc ggaaactggc gaccgtgaaa ctgctctctg gttcaagaag gaagaactgg
      781 ttgagtggtc gcgcgctatt gatgcttggg tatttgaaaa gtaaaaaaca agtaataaaa
      841 aagcccttcc ataggaaggg cttttttatt actttaatct caacagaatt ttcccggctt
      901 cccataagtc ttcgatctgg tagtattcgc gttggtctgg agaaagaagg tgcaagacca
      961 catctccaaa gtcaacgacc acccagccat cacgggaatt gccttgtttt ttggcttttt
     1021 tcttatgttt tttacggact tcatcaatgg cgctgttagc aagcgcatct aacatgcggt
     1081 cactggtgcc ggtacagatg acgaaataat cagtaaaaga agcaatttct ttgatgtcca
     1141 acaggacgat gtcttcgcct tttttatctt cgagagcatc tacaatgttg cgagcaattt
     1201 ctagggtatt cagagttcac ctcaatgggg ttgaaaataa cttcgtatag accgaacctt
     1261 ctgagttcaa gtcactttta taaagatgca cagagtctac tctggctgtg ccaattttgc
     1321 caagttgaaa tgaggaaatc gtattactga tcttctgtaa ttctgtgacg ttaattcctt
     1381 gacggacacg ccctaatgta aggtgcggcg agaaggggcg ttcttctttt tcataaccaa
     1441 ggtgatgagc gccttcttcg acggcttttt ggagtgtggc aagagtggct ggtatgtgga
     1501 ctccgaccca gaggacgcga ggtcgctttg agtttgggaa agagccgaag ccgcttattt
     1561 gtaaatcgaa ctgcgacgat gaatcagctg cttgggtgag catctgtttg atgaattcca
     1621 gatgcgagat ggggatgttg cctaggaatt ttaacgtcaa atgcagatta tgagttggaa
     1681 cccaacggat gaaatcctcc cccaaggttt ggcgcaggcg ctgtgtttgc tgttctattg
     1741 attcgagaat ggataatgga aaatccagag caataaaggt tcggatctga ttcattccta
     1801 gcttgaggct tgcagtacct tctctacagc atttttgagg tcttgaaagc caacgggttt
     1861 ggtgaggtac attgaagccc cagcttcaag cccggttttg atatcgccgg gcatgctttt
     1921 ggctgaaagg ataatgacga gaatggaggc gagttcaggt tcgcggcgaa tgtaacgtaa
     1981 cacttcaaga cctgaaatat cgggcatcat tacatcgagc aaaataatgt ctggcttttc
     2041 ttgtgagatg atgggaatgg cgggagcgct ggagaacatt ttgataacac ggaacccatt
     2101 gatgcgcatc atttctgaaa aaagttcagc ggcatcggct tcgtcttcga tgatcatgac
     2161 cgttttttgt ctcgaattca ttttgtttcc tctactatgc taaaactagc ataaaggtat
     2221 ggaatttgca agggcgcatg atttttgcga tgtccttgta agaattcgga tttgttccat
     2281 gtctatgcaa aataagattt ggaggtatca atgagtgaat tggagctttt tcgtgcagaa
     2341 aagaatgagt tttttggcag ccatccgcaa agtccgctaa cccgtgagca gaaaaaagac
     2401 tttcatgggt tgaattattt tccagaaaat gaaactttac ggctggaagt gaaagtggaa
     2461 gaattttcag ttaaggatga gttggtgatg caaaccacca caggtggtgt tcagcactat
     2521 gaacgctttg gaaaattctc ttttgatgtg gagagtcaaa ccgtagagtt gaccgtttat
     2581 cgtagcgagc atggcttttt tctgccgttc gtggatgcgt tggctggtag tgagacgtac
     2641 cccgccgggc gttaccttga gccagaccct ttgccaggtg ggcgcttctt tattgacttc
     2701 aacttggcat acaacccata ctgtgcctac aatgagatgt ggtcctgccc aatcaccccg
     2761 gctgagaacc gcttgaaggt gcccatacgg gctggtgaga aactattcca tgaataaaaa
     2821 aaatgacggg ctagcccgtc atttttttta ttcatcttga gtattgcttg tattttctac
     2881 aacattcagt aaagcttcat attcgtcaca acagccggaa cacatatcta aatgctcatg
     2941 gatgagtgga gtgagaatgg cggcgtcttt accgtctttg atctcggctt ccacaaattc
     3001 atccagccgc gcatacatgt cactgcaaga gagatcctct tcacgaactt gctcaaggat
     3061 cttcaagaat cgcaaggcaa cttcatccgt aatctcaggt tgagaaggct tgttttgagg
     3121 ggtagggaga tgttcgttat tcattctaaa gggtgagacg attaataaaa acttaatctt
     3181 actttgtcaa ttatacccaa ttgataagaa attatttctg ctcgaaggcg agtagaacat
     3241 cttgcggtgt cagttcttcc agcgctaggc gttttttgag gcgcaggcgg gcatcgtgaa
     3301 gcagtttgta taatgcattg cggttggttt tcatgcgttt tgcggcatct tccatgggca
     3361 tgtcttgcaa gccaagcaaa acgagcgcct ctcgctgttt tggggtcagc tcttcttcca
     3421 agatgcggcg tacacgagag accatatctt tgcgttcggc agaggtttca ggggatgcgt
     3481 gagggtctgc aagcaggccg ggaggaggtg gggcgtcttc attttccgtc aactcatcga
     3541 gagacgcgtc acgccagcgc ttgcggcgaa gttcggtcaa tgcgatacgg atggcaattt
     3601 tgtgtaccca ggtggtgaaa aggctgcgcc cttcaaaggt atccaattgg tctaatacgc
     3661 gtaggagggt ttcctgggtt acttcttcga cgaggggagg aaagagagga tcatctgacg
     3721 atagccagcg tgatagtgcg tacggcaagc ccttttggat gattgcacgc aagtcgctta
     3781 acgcctcttc atggacggga ccagaactgc gtaaatcact taaccaagtt tcattagtgc
     3841 gagaagacat aacgtagtca ttataagata ctgcaagggg tttttcagca tccctgagag
     3901 attctgatag tatttttata aatccgtttc ggaaaagaaa aaaccgcctc aaggagacgg
     3961 ttttctgtgg gcgcgcaggg actcgaaccc cggaccccta ctgtgtgata gtagtgctct
     4021 aaccaactga gctacgcacc caacggagcg agattatagc cgagcctctc atcttttgca
     4081 agcctaatgt gccaggtctt cgggtctaaa acaggcttca tagtctgcac cgatgatgac
     4141 gcggtattga acggtactgt ttgggtctgg catggataaa acattcgctg aataggtacc
     4201 tagcacatca atgatgtttt gacgctggct agggtcttga gccacggtat agtcaaccag
     4261 aacggtgctg ccatagtttc ggttatccgc tgggctggtg gtggtttcgt agccggcgta
     4321 gttgaggcga gaggaagcga gagtttccat ggtgtcgaag tatgagccgt tttgtacatc
     4381 aacttttatt gtttcgcgag tcgctgcata ggtggatagg gtagttgctt cgaccagcat
     4441 ggagcttagc gagtcttggg gtaagagaac ggcagctcca ccgggcgtcg tccacgggag
     4501 gacgtagggt ggacgaatgt agtagctgcg aatgtttgca tt
//
LOCUS       DAOQFT010000142         4533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10161_length_4533_cov_6.202769, whole genome shotgun sequence.
ACCESSION   DAOQFT010000142 DAOQFT010000000
VERSION     DAOQFT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10161_length_4533_cov_6.202769"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..342)
                     /locus_tag="PLR93_04940"
     CDS             complement(1..342)
                     /locus_tag="PLR93_04940"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH26341.1"
                     /translation="MPEKRTVPRKKFNLYMRVLNDDTGEILGHMVEISSTGLRLETVG
                     PLPTNVDYYLRIELTPDLGSMPFIIFVARSKWCKIDTIQPNLFQVGFEVVEMVPEDRE
                     AFLRIMQKYAM"
     gene            complement(407..1723)
                     /locus_tag="PLR93_04945"
     CDS             complement(407..1723)
                     /locus_tag="PLR93_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26342.1"
                     /translation="MKFSDLVRLVINNLSRRKARVALTAIGVVIGTGAVVILVSLAIG
                     LQMNANEQLYGIGDLSQISVVPAYGGGPMPMGGGSAAVAPEDVKLLTNSALQDLRAIP
                     GVTAVIPRDYMFSSSFVSYKKLQGYSNIFGVGTNDLSELGLEAQQGTTALSRATVVIG
                     YMVAQNFMDPNIRPGQIPPPPPDLYDQQIQLTLTKFDDKNNEIRKNYSLRVAGVLSES
                     GSEADYSIYLPIEVVKSMNEWANNSTINYNKTGYNEVLVRVENPDKTLEVRDAITALG
                     FQANTSLDFVQGINNFYSILQIVLGGVGAIALLVAAIGIANTMAMSILERTREIGLMK
                     AVGATNRDVLSLFLGEAAGIGFIGGLGGVLTGWLLGQMLNVVAVVYLAQQAAQQGGVP
                     PSVAIYTPIWLPVFVLAFSTFIGMLSGLYPALRAANMIPVLALKYE"
     gene            complement(1745..3454)
                     /locus_tag="PLR93_04950"
     CDS             complement(1745..3454)
                     /locus_tag="PLR93_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNH26343.1"
                     /translation="MNLVRKSFLWVGVIMALLVSSIPTSVLAADLLSVTPTSVVNTAD
                     KTITVTGTGFDNTAVVLLDGSALATTFYNDQTLTAVVPAGYVPKTYNLTVTMSGGTVN
                     IPTPVSFTVTAPAPTNTPIPTPTAAPFARPQLRVSFSGTSGKDGIVSNKPFKFTVNFE
                     NAGSLPAYNTQATFTSADLVPLDTGGVAVLGSVSAGSSVSVDQRFVSNGSLSGKSIIT
                     IDVTLSYNDEKGNAYSDKFTLSASVSGGGGGSGVYYTATPTGVRSGQLIITSYGTDVD
                     PLEPGSRFNLVMTVQNMGNEKAPRVTMIVGGGSSGSSDGTPQPGGVSGGNGEFTNFAP
                     VGTSNIQSLGDLPAGGMVQAKQDLIVNVSTEPGAYPIKVTFSYLNAKGEVVNDEQVIT
                     LLVYSLPNLDISFYRPLDPFFVDQPGALPIQVVNLGKRTAVLGTITVTTDNGTVDGGT
                     SLVGSLDKGGYYTLDATLTPLQTGPAKINFVIEYTDDFNQKRTVEKTIDVNVEEGFIE
                     PTPDPNVPLDTGGMTPPTEETFLQKTWRFILGLLGLDSAPSTSSPDMQSPPTDFQSVP
                     SGG"
     gene            complement(3455..4183)
                     /locus_tag="PLR93_04955"
     CDS             complement(3455..4183)
                     /locus_tag="PLR93_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH26344.1"
                     /translation="MNSSFIRVVGLKRHYEMGGTLVRALDGVDLEIPSHTFTVVMGPS
                     GSGKSSLLYLLGGLDRPTAGEIYVNGERLDQMDENALALFRRKTMGFIFQSFNLVSSM
                     TALENVAFPMQFAGVPVSQRNEKSQSLLSQVGLAERSHHKPSELSGGQQQRVAVARAL
                     VNDPVLIFADEPTGNLDTQSGVTIMQMLSDLHKSGRTVLVVTHDPRMVRFATNKIFLL
                     DGKVVTEAEYQAVALEPLQGNSGG"
     gene            complement(4185..>4533)
                     /locus_tag="PLR93_04960"
     CDS             complement(4185..>4533)
                     /locus_tag="PLR93_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26345.1"
                     /translation="QSALMITAWASLPFLVRDGLRIIYMLIAQHPIQSPGLSGFTTNA
                     VFASQLLSKVDLFYIWSVVLLVIGFGVTDNMPRNKSISNVVIVSILLLLIQSGVGSLL
                     SGVSGLAIQRPFF"
BASE COUNT         1317 a         1109 c          970 g         1137 t
ORIGIN      
        1 ctacatcgca tacttctgca taatgcgcaa aaatgcttca cggtcttctg gtaccatttc
       61 aacaacctca aaaccaactt gaaacagatt tggttggatc gtatcgatct tgcaccattt
      121 actacgtgca acaaaaatga taaacggcat ggaacctaaa tctggtgtaa gttcaatacg
      181 cagatagtaa tcaacattcg taggtaaagg acccaccgtc tcaagcctta gccctgttga
      241 acttatctct accatgtgtc ctaggatttc ccctgtatca tcgttcaaga cacgcatgta
      301 caagttaaat ttcttgcgtg gaacagttcg tttctctggc atatgatcta cctctgcttt
      361 ccacttgatt tcttagacct aattatacaa ttgatgaaag atttttttat tcgtatttca
      421 atgctaaaac tggaatcatg ttagctgctc gtaaggcagg gtaaagccca gataacatgc
      481 caataaaagt agaaaacgct aaaacaaaga ctggaagcca aataggtgtg taaatcgcca
      541 cactgggcgg aacgccacct tgctgggcag cctgttgggc aagatatacc acggcgacta
      601 cgtttaacat ttgccccaat aaccatcctg tcaacacacc gcctaaaccg ccaataaaac
      661 caatccccgc cgcttcgcct agaaagagac taagaacatc acggttagta gcgccaaccg
      721 ctttcattaa tccgatttct cgagtgcgct ccagaataga cattgccatc gtatttgcaa
      781 tcccaatggc agctacaagc aaagcaatgg cacccacccc accaagcaca atctgcaaga
      841 tcgaatagaa gttattgatg ccttgcacaa aatctaatga agtatttgcc tggaagccta
      901 aggcggtgat ggcatcacgt acttcaaggg ttttatcagg gttttctact cgtactaaaa
      961 cctcgttgta tcctgtcttg ttgtaattta tagtcgaatt atttgcccat tcattcatgg
     1021 acttaaccac ttcaattggg agatagatgg aataatccgc ttcagagcca gactcggaaa
     1081 gaacaccagc aacccgtaaa ctgtaatttt tacgaatctc gttattttta tcatcgaatt
     1141 tcgtcaaggt taattggatc tgctggtcat ataggtccgg cggcggcggt gggatttgcc
     1201 ctggtcgaat gtttgggtcc ataaagtttt gcgccaccat atatccaatg accacggtag
     1261 cacgactcaa tgccgtagtc ccttgctgtg cctctaaccc aagctccgaa aggtcgttcg
     1321 tacctactcc aaatatattg ctatagccct gaagcttctt ataggataca aacgaacttg
     1381 agaacatata atcgcgcgga atcacagctg tcacgccggg gatagcacgt aaatcttgca
     1441 aagcgctatt cgtaagaagt ttgacatctt caggtgcaac cgcggcactt ccgcctccca
     1501 ttggcatggg tcctccccca taagccggaa cgacactaat ctggctaagg tcaccaatcc
     1561 catataactg ttcattagca ttcatctgca agccaatagc tagagacaca aggatcacaa
     1621 ccgcacctgt gccaatgacc acaccgatcg cagtaagggc cacgcgcgct ttacgacggc
     1681 tcaagttatt gatgaccaaa cggactagat ctgagaattt catgcggtag ccagattcat
     1741 catcttatcc gccactcggc acggattgaa agtctgtagg tggagactgc atatcgggag
     1801 aagaagttga cggagcgctg tctaaaccta aaagtccaag aatgaagcgc catgtttttt
     1861 gtagaaatgt ctcttcagtt ggcggagtca ttccacccgt atccagagga acgttcgggt
     1921 caggtgttgg ctcaataaag ccttcttcaa cattaacatc gattgttttc tcaacggtac
     1981 gtttttgatt aaagtcgtct gtatattcaa taacaaaatt tatttttgcg ggtccggttt
     2041 gcagcggagt taaggtcgcg tccaaggtgt aatatccgcc cttatccaat gaaccgacca
     2101 aagatgtacc gccatccact gtgccattat ctgtggtgac cgtgatggtg ccaagcacag
     2161 cggtgcgttt ccccaagtta acaacttgaa tcggcaacgc gccgggttgg tccacaaaaa
     2221 atggatcaag cgggcgatag aaactaatat caagattggg caagctgtaa accaacaaag
     2281 taatcacctg ctcatcatta accacttcgc ccttggcatt tagataagag aacgtgactt
     2341 tgataggata tgcacccggt tcagttgaaa cattgacgat caggtcttgc ttggcttgaa
     2401 ccatgcctcc ggcaggtaaa tctcctaacg attggatatt cgatgtccct accggcgcaa
     2461 aattcgtgaa ctccccattt ccaccagaaa caccgcctgg ttgcggagtc ccatcgctag
     2521 aaccgctaga acctccacca acgatcatcg taacgcgcgg cgctttttcg ttacccatat
     2581 tttgaacggt catcaccaga ttaaatcgag accccggctc aagcggatcc acatctgtgc
     2641 catatgaagt aatgattaac tgcccgcttc ggactcctgt tggagtggct gtgtaataga
     2701 cgcctgatcc gccgcccccg ccggatacag aagcagaaag tgtgaacttg tcagaatagg
     2761 cattgccttt ctcgtcattg tagctcagtg tgacatcaat cgtaataata gactttccag
     2821 ataaagaccc attggaaaca aagcgctggt cgaccgatac gctagaaccc gcagagacac
     2881 ttccaagaac cgctacaccg cccgtatcca aagggacaag gtctgcagat gtaaaggttg
     2941 cctgggtatt gtatgccggc aaagagcctg cattttcaaa atttacagtg aacttaaatg
     3001 gtttgttgga aacaatgcca tctttaccgc ttgtaccaga aaaagataca cgtaactgag
     3061 ggcgtgcaaa tggtgcagcg gttggtgttg gaattggtgt atttgttggt gccggtgctg
     3121 taactgtaaa agaaacgggt gtagggatat taacagttcc accagacatc gtcaccgtaa
     3181 ggttatatgt tttcggcaca tacccagcag ggacaaccgc cgtcaaagtc tggtcattat
     3241 aaaaagtagt cgctaaagct gagccgtcaa gcaatacaac agccgtgttg tcaaatccag
     3301 ttcctgttac tgtaattgtc ttatcggcag tattcacaac agatgttggc gttacagaca
     3361 acaaatccgc agccaaaaca gaggttggaa tacttgaaac caaaagagcc ataatcacgc
     3421 cgacccacaa aaaggatttt cttactaaat tcatttatcc tccagaattc ccctgaagcg
     3481 gttctagtgc aacagcctga tactcagcct ctgtaaccac ttttccatct aacaaaaata
     3541 tcttattggt cgcaaaacgc accatgcgtg gatcgtgcgt taccaccagc acagtccgac
     3601 cagatttatg caggtctgag agcatttgca taatggtcac accgctttga gtatctaggt
     3661 tgccagtagg ctcatctgca aagatcaaaa cagggtcatt taccaaagcc cgtgcaacag
     3721 ccacacgctg ttgttgcccg cctgaaagct cagagggttt atggtgactc cgctctgcca
     3781 gcccaacttg agataagagg gattgggatt tttcatttcg ctgtgaaaca ggcacgcctg
     3841 caaattgcat tgggaaagcc acgttctcta atgccgtcat gcttgaaaca aggttgaacg
     3901 attgaaaaat aaaacccatc gtttttcggc gaaaaagtgc aagcgcgttc tcgtccattt
     3961 gatccaaacg ttcgccgttg acgtaaattt cacccgcagt tgggcggtct aacccaccaa
     4021 gcagatacaa caaactactt ttgcctgagc cggaaggtcc cataaccact gtaaaggtgt
     4081 gagatggaat ctctaaatcc acgccatcca aggcgcggac aagggttcca cccatttcat
     4141 agtgtcgttt taaaccaacg actcgaataa aagaagaatt cattttaaaa gaatggacgt
     4201 tgaatcgcca aaccgctcac ccctgacaat aaagaaccaa cgccagattg aatgagaaga
     4261 agcaagattg aaacaattac aacattcgat attgatttat tgcgcggcat gttatcagta
     4321 acgccaaagc cgatgaccaa aagaacgacg ctccaaatat aaaacaagtc gacttttgaa
     4381 agtagttgag aagcaaatac tgcattggtc gtaaacccag aaagtccagg gctttgaata
     4441 ggatgttgcg caatgagcat atagataatg cgcaaaccat ctcgtaccag aaatggcagg
     4501 ctcgcccatg ctgtgatcat caaggcgctt tgc
//
LOCUS       DAOQFT010000143         4524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10194_length_4524_cov_8.141195, whole genome shotgun sequence.
ACCESSION   DAOQFT010000143 DAOQFT010000000
VERSION     DAOQFT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10194_length_4524_cov_8.141195"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLR93_04965"
     CDS             <1..236
                     /locus_tag="PLR93_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH26346.1"
                     /translation="PLVTVIGLSFGGLLGGAVLTETIFGLSGVGRTLYEAITARDYGI
                     VQVFTLVIAFVFVVINLIVDISYAYLDPRVRLN"
     gene            248..1207
                     /locus_tag="PLR93_04970"
     CDS             248..1207
                     /locus_tag="PLR93_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26347.1"
                     /translation="MTANSTAVATLDPEAITSKSTGMWQLTMRRLLRRRSAMAGLVIL
                     GILIFIAVFAPALAPYDPTISMLDADPPQRIKARVAPCIHLLGCPADQPQHLAGIDGN
                     IRDQFSRLLYGARLSLMVGFSTVTFAIIIGTILGAVAGYFGGWVDNSIMRIMDVLLAF
                     PSLLLAIAIVTVLGPGLINALLAIGIVSIPAYARVMRASVLSVREMEFVSATRALGGN
                     DMQILFLRILPNALTPLIVQGTLGIASAILDAAALSFLGLGAQPPTPEWGSMLGAERN
                     QVFTAPHLVFYPGFMIMLTVLAFNLIGDGLRDALDPRLAHSSG"
     gene            complement(1258..2058)
                     /locus_tag="PLR93_04975"
     CDS             complement(1258..2058)
                     /locus_tag="PLR93_04975"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNH26348.1"
                     /translation="MIQPPASYSDPSLGQQPEPIPTRPPTYVRMPTLQPIVTYALIGV
                     TIFVYLLQVLSSVLFGDSSYGIDWVTLYGARISDAIYAGQLWRLFTPMFLHGSIPHIF
                     FNMYALFSVGSLLERHFGHGRFLLLYVLGGFAGNVLSFLFTQGYSVGASTAVFGLIAA
                     EAIFFYQNQKLFGSHAKQAISNAIFIIAINLFLGLAPSIDTWGHIGGLLGGAMFAWFA
                     GPKWTVVGMPPEYHLDDERESREIITGALLVALIFGGLTVWAFIQHAA"
     gene            complement(2055..2669)
                     /locus_tag="PLR93_04980"
     CDS             complement(2055..2669)
                     /locus_tag="PLR93_04980"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNH26349.1"
                     /translation="MTEFELRKPNPLLIVISGPSGVGKDSVVQRMKDRGFPFHFVVTA
                     TTREKRANETHGVDYFFLSKDEFAHMIEEDELIEYAIVYGDYKGIPKEQVRNAFASGK
                     DVVMRIDVQGAESVRKLAPEALLIFITCESEEELERRLRERKTESPDSLSLRIATARK
                     ELQRLEAFDYVIVNHDFHLDDTVDKVRAIIDAEHLKVHHRMVTL"
     gene            2729..3112
                     /locus_tag="PLR93_04985"
     CDS             2729..3112
                     /locus_tag="PLR93_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH26350.1"
                     /translation="MPTYDFICNTCEKRFEVFLTYSEYGKKTVECTHCNSTDVRRRMT
                     KVRIAKTDEGRLEHMAGDLGSLEGMENDPAAFARRMKKMGKEMGEELPAEFDEVVDRL
                     EAGQSPEEIESAMPDLSGGDELSKE"
     gene            3336..>4524
                     /locus_tag="PLR93_04990"
     CDS             3336..>4524
                     /locus_tag="PLR93_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26351.1"
                     /translation="MSLKKFLVLLGVLVVLGAVLAACGGTQPTQAGTETATEEAPAAP
                     IPDTPYLADWQGSAHANVADEPFRHWDDAAENPDGVPTSCAKCHTTDGYRDFLGADGS
                     EAGKVDVAVAADHAQGVQCVACHNAVTMTKTTVMFPSGVEITAGDDSRCMECHQGRES
                     KVSVDAQIEKFAATDPDAVVAPIKDDQGNDVFFGFRNVHYYAAAATLYGGMTHGGYEY
                     DGLTYDAKNTHTDGYASCTGCHDPHTLEVKVDQCAFCHEDVATVEDLKNIRMVSSAPD
                     YDGDGNVEEGMYYEIEGLQAALLAEIQTYAADKGGAAIIYDSASYPYWFIDTNANGAA
                     DEGEVAFPNAYSTWTPRLLKAAYNYQVSMKDPGAFAHGNKYIVQLLFDSIADLGGDTS
                     KLAR"
BASE COUNT         1081 a         1115 c         1150 g         1178 t
ORIGIN      
        1 tgccgttggt gaccgtgatc ggtttgtcct ttggcggtct cttgggcgga gcggttctca
       61 ccgaaacgat ttttggtctt tctggtgtgg ggcgcaccct ctacgaagcc atcaccgctc
      121 gtgactatgg tattgtgcag gtctttactc tggtgatcgc attcgtcttc gttgtgatca
      181 acctgatcgt ggatatttct tatgcctatc ttgatcctcg tgttcgcctc aactaaggaa
      241 ctcgaaaatg accgctaact caaccgctgt agcaacactg gatcctgaag cgattacatc
      301 aaagtccaca gggatgtggc aactcaccat gcgccgactg ttacgccggc gctctgccat
      361 ggcaggtttg gtgattcttg gtattttgat ttttattgcg gtttttgccc ccgctcttgc
      421 tccatacgac cccaccatct ccatgttgga tgccgacccg ccgcagcgga tcaaagctcg
      481 cgtagcgcct tgtattcacc tcttggggtg tccggcagat caaccccaac atcttgctgg
      541 tattgatgga aacatccgtg accaatttag ccgcttgctg tatggtgcac gtttgagctt
      601 gatggtcggg ttttccaccg tgaccttcgc tatcatcatc ggaaccatcc ttggtgcagt
      661 agctgggtat ttcggcggct gggtcgataa tagtattatg cgcatcatgg atgtgctatt
      721 ggcattccct tctctgttgc ttgccattgc catcgtcacc gtcttgggac cgggtcttat
      781 caacgcctta ttggcaattg gtatcgtatc catccctgct tatgcacgtg tgatgcgtgc
      841 ttcggtgctc tctgttcgtg aaatggaatt tgtctctgct actcgtgctt tgggcggtaa
      901 cgatatgcaa atccttttct tgcgtatttt gcctaatgcc ttgacaccgc tcatcgtaca
      961 agggacgttg ggtatcgcct ctgctatttt ggatgcagcg gctttgtcct tcttgggctt
     1021 gggcgctcaa cctcctacgc ctgaatgggg ttccatgctc ggtgcagaac gcaatcaggt
     1081 ctttaccgcg ccacacctcg tgttttaccc cggctttatg attatgctta cggtgttggc
     1141 attcaacttg atcggtgatg gtctgcgtga tgcgcttgac ccacgccttg cacactctag
     1201 cggataaaac attgaatcaa aaaagcccgt cgtaaagacg ggctttttta ttatttgtta
     1261 tgcagcgtgt tgaatgaatg cccaaacggt cagcccgccg aagatgagcg caacaagcaa
     1321 tgcgccagtg atgatctctc gactttctcg ttcgtcgtcc agatgatatt caggcggcat
     1381 accgaccacc gtccacttgg gacctgcaaa ccaggcgaac attgccccgc cgagtagacc
     1441 gccaatgtgc ccccacgtat caatgctggg agccaagccg aggaagagat tgatggcaat
     1501 gataaagata gcattgctaa tggcttgctt ggcatgtgaa ccgaataact tttggttttg
     1561 gtaaaagaag atggcttctg cggcaatgag accaaatacc gctgtggaag caccgacgga
     1621 atagccttgg gtaaagagaa aagacaatac attcccggca aaaccaccga gtacgtacag
     1681 caacaagaag cgtccatgcc cgaagtgccg ttccagcaga ctcccaaccg agaaaagcgc
     1741 atacatatta aagaaaatat gcgggatgga gccatgcagg aacatgggcg tgaacaaacg
     1801 ccaaagttgc ccggcataga ttgcgtcact gatgcgggcg ccatataaag tcacccaatc
     1861 aatgccgtag ctagagtcac caaaaaggac agagctgaga acttgcaaga ggtacacgaa
     1921 aatggtcaca ccgatgagtg cataggtgac tatgggttgc aaggtgggca tccgcacgta
     1981 agtgggcgga cgggtaggaa tcggttcggg ttgttgaccc agtgacgggt ccgaataaga
     2041 ggcgggaggt tgaatcacaa ggtcaccatg cggtggtgca ctttcaaatg ttcggcatca
     2101 atgatcgcgc ggactttatc cacagtgtca tccaaatgaa aatcatgatt gacgatcaca
     2161 tagtcaaagg cttcgagccg ttgcagttct ttgcgggcgg tggcaatgcg tagagaaagg
     2221 gagtctggcg attcggtctt tcgttcgcgc aaacgtcttt ctaattcttc ttcgctttcg
     2281 cacgtgatga aaatgagcaa agcttcaggc gcaagcttgc gaaccgactc agctccttgc
     2341 acgtcgatgc gcatgaccac atctttgccg ctggcgaagg cattgcgcac ctgttctttg
     2401 ggaatgcctt tgtaatcgcc atagacgatg gcatattcga tcaactcatc ttcttcgatc
     2461 atgtgcgcaa attcatcttt tgagaggaag aaataatcca cgccatgggt ttcgttggca
     2521 cgcttttcac gagtggtggc agttaccaca aagtgaaaag gaaacccgcg gtctttcatg
     2581 cgttgcacca cagagtcttt gcctacacca gaaggaccag agatgacgat caacagcgga
     2641 ttgggtttac gaagctcgaa ttcagtcatc tgcgtattgt accaaacgcc tcttgccaac
     2701 ctctggcttg attctgtaaa atcagtctat gcctacttat gactttatct gcaatacctg
     2761 tgaaaagcgc tttgaagttt ttttgacata tagcgaatat ggaaagaaaa cggttgaatg
     2821 tactcattgc aacagcacag atgtgcgccg ccgtatgacg aaggtccgca ttgcaaaaac
     2881 ggacgaaggc agacttgaac acatggctgg tgacctaggc agtttggaag ggatggaaaa
     2941 tgaccctgcc gcatttgctc gtaggatgaa aaagatgggg aaagagatgg gcgaagagtt
     3001 gccagccgag tttgacgaag tggtggatag gctggaagct gggcaaagcc ccgaagagat
     3061 cgaatcagcc atgccagacc ttagtggggg cgacgagctt tctaaggaat aaaccctttc
     3121 cccgacgtat aatttcacta atcttatcta aattgttgtg acttttgtca cattaaggga
     3181 tgcttatttt tactgtgcat taatctccca cttaagtaaa atttctcata tctgctaaaa
     3241 aatgtcaaag cagtcatttt ctccaaagga ggcatatttt caagaacacg ccgatattta
     3301 ccagcttttt tacactgatc taaggagaat gaataatgag tctcaaaaaa tttctagttt
     3361 tgctcggggt tttggttgtg ctcggcgcgg ttttagcggc ctgtggcggc acccaaccca
     3421 ctcaagcagg tactgaaaca gccacggaag aagctcccgc tgctccaata ccagatactc
     3481 catacttagc tgattggcaa ggctctgctc acgcgaatgt agcagacgaa cctttccgtc
     3541 actgggatga cgctgccgaa aatcctgacg gcgttcccac atcctgcgct aagtgccata
     3601 ccaccgatgg ttatcgtgat ttcctcggtg ccgatggttc cgaagccggt aaagtggatg
     3661 tagccgttgc tgccgaccat gcccagggcg tgcagtgcgt ggcttgtcac aacgctgtaa
     3721 ccatgaccaa gaccacggtc atgttcccct ccggtgttga aatcacagcc ggtgatgata
     3781 gccgttgtat ggaatgtcat cagggacgtg agtccaaggt cagtgtggat gcgcagattg
     3841 aaaagtttgc cgccaccgac cccgatgccg ttgttgctcc gatcaaggat gaccagggca
     3901 atgatgtgtt ctttggtttc cgcaatgttc actactatgc tgcagctgcc accctctacg
     3961 gtggtatgac ccatggtggg tatgaatatg atggcttgac ctacgatgcc aagaacaccc
     4021 acactgatgg ttatgcttcc tgcacaggtt gtcatgatcc tcataccctc gaagtcaaag
     4081 ttgaccagtg cgctttctgt cacgaagatg tagccaccgt ggaagatctc aagaacatcc
     4141 gcatggtttc atccgctcct gactatgatg gcgatggcaa tgttgaagaa ggtatgtatt
     4201 atgagatcga aggcttacaa gctgctttgt tggctgagat ccaaacatat gccgctgata
     4261 agggtggcgc tgcgatcatc tacgattcag catcctatcc ctattggttt atcgacacaa
     4321 atgccaatgg cgcagccgat gaaggtgaag ttgccttccc gaatgcgtat tccacctgga
     4381 ctcctcgttt gctcaaggct gcttataact atcaggtctc tatgaaggat cctggtgcgt
     4441 ttgcccacgg caacaagtac atcgttcagt tgctcttcga ctcgatcgcc gacctcggtg
     4501 gcgataccag caaactcgct cgta
//
LOCUS       DAOQFT010000144         4508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10257_length_4508_cov_3.501909, whole genome shotgun sequence.
ACCESSION   DAOQFT010000144 DAOQFT010000000
VERSION     DAOQFT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10257_length_4508_cov_3.501909"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLR93_04995"
     CDS             complement(<1..295)
                     /locus_tag="PLR93_04995"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HNH26352.1"
                     /translation="MPRKKTTLPPQPPQEEDSVWTYRGYKLKSSEFVTAMVHLFRAEV
                     QRANVWRQRLDTTTNWAVVATGATLSISFSQPNVHHGVIILNTLLVTWFLFIEA"
     gene            complement(301..2100)
                     /locus_tag="PLR93_05000"
     CDS             complement(301..2100)
                     /locus_tag="PLR93_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNH26353.1"
                     /translation="MSQTSLTLLLLGVAAILLLRNILRADIVALMLMLALGLSGILTP
                     QEAFSGFSRQAVITMLSAFILAEGLRRSGMTERIGLFIIKLFGAGERRLIFGVMTASA
                     VLSLFMNNIAAASLLLPSISGIAHKMKISPSKLLMPLAFGTILGGMATLLTTVNLVVS
                     GLLRDENLPAFSLTDFLPMGLTLTFVGILFMVLWGRKLLPNQSPAQRHEEENASVELI
                     KTYQLAERLVRAEVCKGGRMDGVTLEKSGLREKYHLNVIAIQRRRNTLPIEAKTIIKG
                     DDILLIVARPEDSLPQSLGEMLNILPSGQWEQDYLSTPNMKLIEAAIAPRSHLASQTL
                     SEIRFEQKFNSKVLAIWRHGRSIRTRLADLPLEFGDGLLMQGTEKALSLLQTEPGLIL
                     LAESAPSTRMTWRNWLTFLIMFATMVLAVVFSDNIAEIMLTGSVMMVLIGTMNMDQAY
                     RSIDWRSLFLVAGMLPLGVALNKTGASTLFANAILAELGDANHLVLLASFVLLTVVLT
                     QIINGAAAVTVIAPIAIATSLQVGMEPRSVAMAVALASSMAFMSPLGHSVNVMVMGAG
                     GYTFKDYARVGIPLTILLVIILLFVLPILMPIG"
     gene            complement(2280..3677)
                     /locus_tag="PLR93_05005"
     CDS             complement(2280..3677)
                     /locus_tag="PLR93_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH26354.1"
                     /translation="MYSQQFFIDGLGCASYIVGCEAKGVAAVVDPDRDVQKYLDAAAS
                     RGLAITHIIETHLHADHVSGNSDLSAKTGAKIYVHEASGAEFQHEVVREGDVLELGNL
                     RLTVMHTPGHTPESITLLVTDTTRSDQPWLALTGDTLFVGDMGRPDLVGAEAANGLAS
                     DMYNTLFEKFLPLNDGLLIYPGHGAGSLCGKSIGSMRSTTLGYERISNPALTPRSKEE
                     FVQYAISDLPEQPGNHTRIKAMNRKGVKALGIVESKPLSIQEAVPFFQRGAGLLDTRS
                     KSAYVQAHIPGSVHLEANDQLSNRIGFVFPPDVPMILLLDNPADYERVVYSLARVGYE
                     NVVGYLSEGLDVWERMGLPLAAGDVKDIEPRELYNIMQSCQNGDCPIVVDVREPWEYK
                     QGHVPGAILMPLGQLSKRVSELDTEHPVAVICASGSRSQSAAALLGQKGFKTIYNVVG
                     GTGAWMYDNLPISRD"
     gene            complement(3698..>4508)
                     /locus_tag="PLR93_05010"
     CDS             complement(3698..>4508)
                     /locus_tag="PLR93_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNH26355.1"
                     /translation="LSTFAVVCIFILAPNSKANNLLGMELLLGFAIGLSLGLLGGGGS
                     ILMVPALVYLLGQTSQAAVTTSLAVVGANSLMGANFHRTQGTLNWRVALVFGWVGMLT
                     AFLASGFSKLIPSTVLLIAFAVLMLVIGSFLLIRKEVKNTVELERPLWVTLVSGAAVG
                     ILTGVLGVGGGFLIVPALVILVGLPMQIAVGTSLVIITMNSFAGFLGHIHDSTIQPVL
                     ILVFVISGLLGTFVGSRLAKFLPAKKLQTAFAWFVILLAIFLLVDNLKRLY"
BASE COUNT         1334 a         1159 c          963 g         1052 t
ORIGIN      
        1 gggcttcaat aaataggaac catgtaacta atagtgtatt taaaataata acgccatgat
       61 gtacattggg ctggctaaaa gaaatggaaa gcgttgcgcc ggtggcaacc accgcccaat
      121 tggtggtggt atccaagcgc tgacgccata catttgcacg ttgcacttct gcacggaaaa
      181 gatgcaccat ggcagtgaca aattcactgc ttttgagttt atatccgcga tatgtccaaa
      241 cggaatcttc ttcttgtgga ggttgaggag gtagggttgt cttttttctt ggcataaagt
      301 ttatccaatc ggcataagaa tggggagaac aaacaaaaga ataatgacaa gcaaaatggt
      361 aagagggatg cctacccttg cgtaatcttt gaaggtatat ccaccagcgc ccattaccat
      421 aacattgacg gaatgcccaa gtggtgacat gaatgccatg gaagaagcca atgctaccgc
      481 catggctaca ctgcgcggct ccatcccgac ctgcagactg gtggcaattg cgatgggagc
      541 gattacagtg acggcagcag caccattaat gatctgggtc aacacaaccg taagcaaaac
      601 aaaactcgcc agcaatacca ggtgatttgc atccccaagt tcagctagaa tagcatttgc
      661 aaacagcgtg gatgctcctg ttttattcaa tgcgacaccc aacggtaaca tcccagccac
      721 cagaaacaag ctacgccaat caatggaccg ataggcttgg tccatattca tggtaccaat
      781 gagcaccatc atcacagaac ccgtcagcat gatctctgca atgttatcgg agaatacaac
      841 tgccagtacc attgtggcga acatgatcaa gaacgtcagc caattacgcc atgtcatgcg
      901 agtagatggg gcagactctg ccaacaagat taacccaggt tctgtctgca acaaactcaa
      961 agctttttct gtgccctgca tcaaaagccc gtcaccaaat tcaagcggta gatccgccag
     1021 ccgtgtacgg attgaacgtc cgtgccgcca aatggctaac acctttgaat tgaacttctg
     1081 ctcgaaacga atttcagaca acgtttgtga agcaagatga gaacgcggcg cgatggcagc
     1141 ttcaattaat ttcatattgg gtgtggaaag ataatcctgc tcccattgcc ccgatggcaa
     1201 tatattcaac atctccccca gtgactgtgg caaactatct tcggggcgag ccacaatgag
     1261 taaaatatca tcgcctttta ttatggtctt tgcctcgatc ggtaatgtgt tgcgtctgcg
     1321 ctggatggcg atgacattga ggtgatattt ttcccgcaaa ccgctttttt ccagtgtcac
     1381 accatccata cgcccacctt tgcatacttc tgcacgtact aaccgttcag caagttgata
     1441 ggtcttgatc aactcaactg aagcattctc ctcttcatgc cgctgagcgg gcgattggtt
     1501 cggtagtaat tttcgccccc ataaaaccat gaaaaggatt cctacaaatg tcagcgtcag
     1561 ccccataggc agaaaatctg tcaaagagaa cgcaggtaaa ttttcatcgc gcaacaatcc
     1621 gctcaccaca aggttgaccg ttgtcaacaa ggttgccata cctcccaaga tcgttccaaa
     1681 tgccagtggc atcaacaatt tcgatggaga gattttcatc ttgtgcgcta taccagatat
     1741 cgaaggcagc aataaagatg ctgcggcaat attattcata aacagcgaca acacagcaga
     1801 tgcggtcatc acaccaaaga ttaatcgacg ctcgcccgcg ccgaacaact taataatgaa
     1861 aagtccaata cgctcggtca taccagagcg gcgcaacccc tccgcaagga tgaacgcgga
     1921 aagcatggta attacagcct gtcggctgaa tcctgagaag gcttcctgcg gagtcaaaat
     1981 tccgctcaaa cccaaggcga gcatcaacat caacgcaaca atgtcagccc gtaaaatatt
     2041 ccttagcaac agaattgctg caacaccaag taacaataag gttaaactag tttgtgacat
     2101 atgattttgg acggtttgat gcttaattca tgcaataaat gagtttacac ccactaaaga
     2161 aaatacttcg tgggtctttc attacgattg ctgtcaaaat catgagacac atttacttcc
     2221 cgatctagtc gctaacctta gaataaaaca gcccagtgaa atcactgggc tgttttattc
     2281 taatctcgag aaatgggcaa gttatcgtac atccacgcgc cagttccacc gaccacattg
     2341 taaatagtct tgaatccttt ttgacctagc aaagccgccg cagattgact acgactgccg
     2401 cttgcacata tcaccgccac agggtgctct gtatctaatt cagaaactcg cttggaaagt
     2461 tgtccaagcg gcataaggat tgccccaggg acatgccctt gcttgtattc ccaaggctca
     2521 cgtacatcca ccacgatcgg gcaatcgcca ttttggcacg attgcatgat gttatacagt
     2581 tcgcgcggtt caatatcttt cacatccccc gccgcgagcg gaagccccat acgctcccaa
     2641 acatccaaac cttcagagag ataccccact acattctcgt atcctacacg tgccaaactg
     2701 tacaccacac gttcatagtc tgccgggtta tcaagtaaaa gaatcattgg tacatctggc
     2761 ggaaatacaa acccaattcg gttgctcaac tgatcattcg cttcaagatg aacagaaccg
     2821 gggatatgtg cttgcacata ggctgattta gaacgagtat cgagcaagcc agccccacgt
     2881 tgaaaaaaag gtaccgcttc ttgaatcgac aagggtttgg attcaacaat cccaagcgct
     2941 ttcacacctt tgcggttcat tgccttgatg cgggtatggt tccccggttg ttctggcaaa
     3001 tcgctgatag catactgcac aaattcttcc ttcgagcgtg gtgtcaatgc ggggttcgaa
     3061 atgcgctcat aaccaagcgt ggtcgaacgc atggaaccaa ttgactttcc gcacaatgaa
     3121 ccagctccat gacccggata gatcaacaaa ccatcgttca atgggaggaa cttctcaaac
     3181 agagtgttgt acatatcact ggcgagcccg ttcgcggctt cggcacctac aaggtctggt
     3241 cgacccatat caccgacaaa caaagtatca ccggtcaaag ccagccaagg ttggtcagaa
     3301 cgggtggtat cggtgacaag caaagtaatg ctttcagggg tgtgccccgg tgtgtgcatt
     3361 acagtgagac gcaaatttcc caattccaac acatccccct cacggacaac ttcatgctgg
     3421 aattcagcac cacttgcttc gtgaacatag atcttcgcac ctgttttagc tgataagtca
     3481 ctattcccag agacatgatc agcgtgcaaa tgtgtttcga tgatgtgggt tatggctaac
     3541 ccacgagaag cagcggcatc aagatatttc tgaacatcac ggtctgggtc gacaacagca
     3601 gccacccctt ttgcttcaca tccaacaata taagatgcac acccaagccc atcaatgaag
     3661 aactgttgtg aatacatatg tgctcctcca aataccttta atagagtctt ttcaaattat
     3721 ctaccaacaa gaaaattgcc agcagaatga caaaccaagc aaaagctgtt tgcaattttt
     3781 tcgcaggcaa gaactttgcc aaccgtgagc caacgaaagt tccaagtaaa cctgatataa
     3841 caaacaccaa aatcaacaca ggctgaatag tgctatcgtg aatatgccct aaaaaacctg
     3901 caaagctgtt catagtgatt ataactaacg aggtaccgac tgctatttgc atcggcaacc
     3961 cgactaagat tacaagggct ggcacaatga gaaatccgcc ccctacgcca agcacaccgg
     4021 tcaatattcc aacggcagcg ccactgacta aggttaccca aagaggtctc tcaagttcga
     4081 ctgtattttt gacttccttg cgaataagaa gaaaacttcc aatgacaagc atgagaacag
     4141 cgaaggcaat caacagtaca gtggatggaa tgagtttgga aaagcccgag gcgaggaatg
     4201 ccgtcagcat accgacccat ccaaagacca gcgcgacgcg ccagtttaac gtcccctgtg
     4261 tacgatgaaa gtttgctccc atcaaactat tcgcacctac caccgccagc gacgtggtca
     4321 ctgcggcttg cgaagtttgc ccaagcaaat aaactaatgc agggaccatc agaattgaac
     4381 ctcctccgcc aagcaaccct agcgaaagcc cgattgcaaa accaagcaga agttccatac
     4441 cgagcaagtt atttgccttt gaattgggtg cgagaataaa gatacatacc accgcgaacg
     4501 ttgacaac
//
LOCUS       DAOQFT010000145         4505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10267_length_4505_cov_5.025393, whole genome shotgun sequence.
ACCESSION   DAOQFT010000145 DAOQFT010000000
VERSION     DAOQFT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10267_length_4505_cov_5.025393"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLR93_05015"
     CDS             complement(<1..486)
                     /locus_tag="PLR93_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH26356.1"
                     /translation="MPVDYEDALDYSSNWMEWIQKNEFPTPEQAHKIIEESTTNFAEY
                     YNRNWLEYRKSVTESGDWAAVEWGGSGAVFKDVLGREYLDFLGGYGMMDLGWSHPEVV
                     STVKAQLERSPMPSQELLDPLRGVLGRLLASITPGDLKYSWFGASGTEANEAAMKIAK
                     LY"
     gene            complement(511..936)
                     /locus_tag="PLR93_05020"
     CDS             complement(511..936)
                     /locus_tag="PLR93_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH26357.1"
                     /translation="MIKKILLPTDGSEHAEKTIHFAIDMAKSLGASLDVMYAYQPAPF
                     LRKRAAAMMEEYRNILEEDAKEVVTEVADRLKTEGLVVNAVVVEGPAAEAILRAADEI
                     KPDLIIMGSRGEGGFADMLLGGVAEHVIHYSKVPVLVVK"
     gene            complement(948..2369)
                     /locus_tag="PLR93_05025"
     CDS             complement(948..2369)
                     /locus_tag="PLR93_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNH26358.1"
                     /translation="MTVEVNPPASGSEIVVKVIKRKITLLPLVMILFFTVSGGAYGLE
                     DLVGSSGPGMAILLIIVTPLIWSLPTALMVAEMSTALPVQGGYYAWAKVALGPFWAFS
                     EGWLSWMTSWVDMAIYPVLFADYLSALLVQQFNIHIIEENALVHWGVTLVVIWVFAYL
                     NIRGAKSIGDSSNLFGLFILAPFLVMSVIGIVKLILHPVAIWEPFVPPDSNILGAFGI
                     GMFVVMWNYLGWDNTSTVSAEVDNPRRNFPRALAITIPLVTLAYLLPVVAGLGSSPDW
                     AGWTAGYFPEVAANIGGKWLGAWLAIGGLVSAVGLFSAIMLSISRLPFVMAEDGYLPK
                     AITKIHPTFETPWVSIVVCAAIYSIFTLSAFSSLVVVDVILYSVALTLEFASLIALRI
                     KAPNMKRPFKVPGGWLGIFLICVFPAGVLALAVVSTIQEEGISALYMSIGAIALTPVM
                     YVILRKFVKKDQPDVEVPVELEG"
     gene            complement(2379..3500)
                     /locus_tag="PLR93_05030"
     CDS             complement(2379..3500)
                     /locus_tag="PLR93_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNH26359.1"
                     /translation="MHKNRFASMFLVFAIVSGMLAACAPSAPVVTSKELNLYAFSEYV
                     PADLLSSFEKETGIKVNYEAYSSNEEMLAGLDANPDKYDLLIPSDYTVEGLIQKKALR
                     PLDLKLIPNYSNIDPAFLSPYFDPGGDTGGRRPYATDNDKYSLPYQWGTTGIAYDTTK
                     VDQPITKWEDLWRPELAGHIVVLDDARELMGAALLSLGYDKNTTDPKRLEEARDKLKK
                     LAPGILAYDSDAPEKYLISGEAWVGVVFNGNAALAMRQNQNIQYVFPEEGATIWFDNF
                     VIPVNALHADAAEAFINFSLAPENSALITRDFPYSSPNTAALDYLAKNDASLYEQYRS
                     NNASNPPPDALANARLAKNLSADASDLYTQFWSEVKNTK"
     gene            complement(3529..>4505)
                     /locus_tag="PLR93_05035"
     CDS             complement(3529..>4505)
                     /locus_tag="PLR93_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH26360.1"
                     /translation="AGNTYIVKPSEKVPMTMQFIFKLIEQVGFPKGVVNMVNGAKDTV
                     DAILEHPTIRAITFVGSTNVAKYIYATAASHGKRVQAQGGAKNPVIILPDADMEMATK
                     IVADSAFGCAGQRCLAVSLAVTVGESKNEFTELICDAASSRTVGYGMDSGVQMGPVIN
                     TASQQRVEQLITLGVKEGAGVPVDGRGTKVKGYEGGSFVRPTILSDVQPGSEIARTEI
                     FGPVLSLMHVKTIDDAIQLVNSGQYGNQASLFTTSGNAARRFRYEADAGNIGINIGVA
                     APMAFFPFSGWKDSFFGDMHGQSTDAVEFFTQKKVVVERWPKEWTRKF"
BASE COUNT         1189 a         1231 c         1020 g         1065 t
ORIGIN      
        1 gtataacttg gcgatcttca tcgcagcttc gttggcttcg gttccgcttg caccgaacca
       61 gctatatttt aaatcgcccg gtgtgataga tgccaataac ctccctaaca caccacgcaa
      121 cggatcaagc agctcttgtg atggcatggg tgaacgctcc aactgcgcct tgaccgtact
      181 caccacctca ggatgactcc agcccaaatc catcatgcca tagccgccaa ggaaatcaag
      241 atactcgcgc ccaagcacat ctttgaatac cgcgccagac ccgccccact ccactgcagc
      301 ccaatcgccc gactcagtta ccgatttacg gtattcaagc cagttgcggt tgtaatactc
      361 agcaaaattg gtcgtcgact cttcaataat cttgtgagct tgttcagggg tcgggaattc
      421 atttttctga atccactcca tccagttaga tgaataatcc aaagcgtctt cgtaatctac
      481 gggcatacac aaatctccat tttagaaata ttatttaaca accaacacgg gtacttttga
      541 gtaatgaatt acatgctcgg caaccccgcc caaaagcata tctgcaaagc cgccttcgcc
      601 tcgcgaaccc atgatgatca ggtcaggctt gatctcatct gctgcacgca aaatcgcttc
      661 tgcggcagga ccttcaacta ccacggcgtt gacaaccagt ccttctgttt ttaaacgatc
      721 agcaacttct gttacaactt ccttcgcatc ttcttcaaga atattcctgt attcttccat
      781 cattgcggcg gcgcgtttcc gcaaaaatgg agccggctga tacgcataca tcacatccaa
      841 acttgcaccc aatgactttg ccatatcaat ggcaaagtga attgtctttt cggcatgttc
      901 cgaaccatcc gtaggaagta atattttttt aatcatgtcg gtaccctcta tccttccagc
      961 tcaaccggta cttcaacatc cggctggtct ttctttacaa atttcctcaa tatcacatac
     1021 atcaccggcg tcagggcgat cgcgccaata gacatataca gcgcgctgat accttcctct
     1081 tgaatcgtgc tgaccactgc aagagccaac acgcctgccg ggaaaacaca aatcaagaat
     1141 attcccaacc aaccacccgg cactttgaat gggcgtttca tattgggagc cttgatgcgt
     1201 agtgcgatca aagaggcgaa ttccaatgtc aacgctactg aatacaaaat cacatcaaca
     1261 accacgaggc tggagaatgc gctcaaggta aagatcgaat aaattgcagc acaaaccaca
     1321 atggagaccc aaggagtttc aaacgtgggg tgaatttttg taattgcctt gggcaaataa
     1381 ccatcctctg ccatgacaaa gggcagacgc gaaatggaaa gcatgatggc actgaacaaa
     1441 cccacggcgc tgaccaaacc gccaattgcc agccaggctc caagccattt cccaccgata
     1501 tttgccgcca cttccgggaa gtaccccgcc gtccaaccag cccaatcagg cgaagagcct
     1561 aaacctgcaa ccactgggag caaatatgca agcgtaacca gcggaatggt aatcgccaag
     1621 gcacgcggaa aattacgccg tgggttatcc acttctgcac taactgtgga tgtgttatcc
     1681 cagccaaggt agttccacat cacaacaaac ataccaatgc caaatgcgcc taaaatattt
     1741 gaatccggcg ggacgaacgg ttcccagatc gccactggat gaaggatcag cttcacaatt
     1801 ccgatcactg acatgacaag aaacggagcc agaataaaca agccaaacag gttcgatgag
     1861 tcaccaatgc tttttgcgcc gcgaatattg aggtaggcaa aaacccagat cacaaccaaa
     1921 gtcacacccc agtgaacaag ggcattttcc tcgatgatat gaatattgaa ttgctgcacc
     1981 agcaacgcag aaagataatc agcaaacaat acggggtaaa tcgccatatc cacccaggat
     2041 gtcatccagc taagccagcc ttctgaaaat gcccagaacg gaccaagcgc aacttttgcc
     2101 caagcgtaat acccgccttg taccggcaag gcggtggaca tttctgccac catgagcgcg
     2161 gttggcagac tccaaatgag cggcgtcacg atgatcagca agatcgccat gcccgggcca
     2221 gacgagccga caagatcttc aagtccgtag gcaccaccgc taacggtaaa aaacaggatc
     2281 atcaccagcg gcaagagtgt gatcttgcgc ttgatgacct taactacaat ttccgaacca
     2341 ctggctggag gatttacttc gacagtcatg tcaggctcct atttcgtgtt ttttacttca
     2401 gaccagaatt gtgtgtaaag atcggatgca tcagcactga ggttctttgc tagacgggca
     2461 tttgccagcg catcgggcgg cgggttggat gcattgtttg aacgatattg ttcgtaaagt
     2521 gacgcatcat tcttcgctaa ataatcaagt gcagccgtat tggggctgga ataaggaaaa
     2581 tcacgtgtaa tcaaggcgct gttttccggc gcaagtgaaa aattgataaa cgcctccgcg
     2641 gcatccgcat ggagggcatt tactggaatg acaaagttgt cgaaccaaat ggttgcgcct
     2701 tcttcgggga aaacatattg aatattctga ttctggcgca ttgccagcgc ggcatttcca
     2761 ttgaacacca cacccaccca ggcttcgcca gaaatcaaat atttttcagg cgcatctgaa
     2821 tcataagcaa gaatacccgg agcaagcttc ttaagcttat ctctcgcctc ttccagccgt
     2881 ttggggtcgg ttgtattctt atcataacca agtgaaagca gtgcagcgcc catcaattcg
     2941 cgcgcatcat ccaagaccac aatgtgcccg gcaagttcgg ggcgccataa atcctcccac
     3001 tttgtgatcg gctggtcgac cttggttgtg tcataggcaa tgcccgttgt gccccattga
     3061 tagggcaggc tgtatttatc attatcagtg gcatacgggc gtcgtccacc cgtatcgcca
     3121 ccggggtcaa aatagggaga aagaaatgca gggtcgatat tgctatagtt tgggatcaac
     3181 ttcaagtcga gaggacgaag cgcttttttc tggataagtc cttcaaccgt gtaatcgctc
     3241 ggaatcaaca agtcatattt atccgggttc gcatccagcc cggcaagcat ctcttcattg
     3301 gatgaatatg cttcgtaatt aacctttatg cctgtttcct tttcaaaaga agagagcagg
     3361 tctgcgggaa catattctga gaacgcatac agattcaatt ctttggaagt cacaaccgga
     3421 gccgagggtg cgcacgcggc aagcattcca cttacgattg cgaacactaa aaacatagat
     3481 gcgaagcgat tcttatgcat atttctcctc tcccatattt tcgggaaact aaaacttcct
     3541 cgtccattct ttgggccagc gttcgaccac aacttttttc tgcgtaaaga actcaactgc
     3601 atcggtactt tgtccgtgca tgtcgccgaa gaatgaatct ttccaaccgc tgaaggggaa
     3661 gaaagccatc ggcgctgcga cgccaatatt gatgccaata ttgcccgcat ccgcttcgta
     3721 gcggaagcgt cgggctgcat ttccgctggt ggtgaacaaa ctggcttgat tgccatactg
     3781 cccgctattg accaactgga tggcatcatc gatcgtcttg acatgcatga gactaagcac
     3841 tggtccgaaa atttcagtgc gggcaatttc gcttcccggc tggacgtctg acagaatcgt
     3901 cggtctgaca aaggatccac cctcgtagcc tttcaccttc gtcccgcgcc catcgactgg
     3961 gactcccgcg ccttccttta caccaagggt gatcaactgc tccacacgct gttgtgacgc
     4021 tgtgttgata accggtccca tctgcacgcc agagtccatg ccgtagccga cggtacgaga
     4081 agacgccgca tcacagatca actcggtgaa ttcgttcttt gattcgccca cggtcacagc
     4141 gagagaaact gccaggcagc gctgacccgc acacccgaag gctgagtcgg cgacgatctt
     4201 cgtcgccatt tccatatcgg catctggcaa aatgatgact ggattcttcg ctccaccttg
     4261 ggcttgcaca cgcttgccgt gtgaggcggc ggtggcataa atgtatttcg ccacgttggt
     4321 cgaaccgacg aaggtgatcg cgcgaatggt gggatgttca agaatcgcat ccacggtatc
     4381 cttcgcgccg ttgaccatgt tgaccacgcc cttggggaaa ccaacctgct cgatgagctt
     4441 gaaaatgaac tgcatcgtca tcggcacttt ttcagacggc ttgacgatgt aggtgttgcc
     4501 cgccg
//
LOCUS       DAOQFT010000146         4503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10274_length_4503_cov_6.727293, whole genome shotgun sequence.
ACCESSION   DAOQFT010000146 DAOQFT010000000
VERSION     DAOQFT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10274_length_4503_cov_6.727293"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLR93_05040"
     CDS             <1..188
                     /locus_tag="PLR93_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26361.1"
                     /translation="EAFDAMKAESEAWIVKCLNCGHERSIWSMGGIRYKATGNSRLYR
                     ICPNCSQRSWQTISKRK"
     gene            complement(220..1068)
                     /locus_tag="PLR93_05045"
     CDS             complement(220..1068)
                     /locus_tag="PLR93_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26362.1"
                     /translation="MINWQELTIDMFVEQLRTSYRRTYGDIQSEYGNIVSWVGRLALE
                     NISNSDALYHDIDHTIMVSLAGLAIIEGKHLREGGVSARDWMRYMIAVLCRDIGYVKG
                     VCKNDTDELFATGEGTERIKISKDGTDVALTPYHVNRSKLFVMERFGSGLLKDMEEHL
                     NAELITTYIEMTRFPSPKGEFYQDTQGLGGLVRAADFVGQLGDPDYFRKTPALFYEFQ
                     ELGANEKLGYQSPNDMRKNYAKFYWEQVNPYLKDALVYLRLTEDGKQWIANMHSHVFD
                     SEHTYK"
     gene            1155..1448
                     /locus_tag="PLR93_05050"
     CDS             1155..1448
                     /locus_tag="PLR93_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26363.1"
                     /translation="MTKRRIFLNWFLIGLSIFLLLNILASALSSDCGLPALFGLGGCA
                     DAISRIGFPFVFFEQGGFAYHSSFNLPVFIIDIAIGIGFAVFLGSYKSRKSNS"
     gene            1449..2369
                     /gene="miaA"
                     /locus_tag="PLR93_05055"
     CDS             1449..2369
                     /gene="miaA"
                     /locus_tag="PLR93_05055"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNH26364.1"
                     /translation="MTKPPVILIVGPTAVGKTELALRLAEQMNGEIVSVDSRLFYRGM
                     DIGTAKPSKEEMARVPHHLIDIVTPDETLSLAVFQEMAQTIIAGIHARGRLPFLVGGT
                     GQYIRAVTQGWSPPEVTPNEQMRAVLETLQAEKGKEWLHAKLQILDPEAAQAIDLRNV
                     RRTIRALEVIFSTGRRFSEQRGIGDSPYHLITIGLARPREELYQRVDERIEKMFADGL
                     LNEVKSLLDQGYSPTLPSMSAIGYRECVSVVQGSMTEEQAKVEMRRLTRVFVRRQGNW
                     FKESDPSIHWFHPARENLLNEIEAMIRNSI"
     gene            2471..4171
                     /locus_tag="PLR93_05060"
     CDS             2471..4171
                     /locus_tag="PLR93_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNH26365.1"
                     /translation="MSNKPWLAHYDKGVPQTIEYPKAPLFHFLEEAARKYPDRACTIF
                     KGAVISYREMNERSNAVAAALVEMGVKKGDRVGIFMPNLPQFVSVYFGILKAGGVVIA
                     VNPTYPVEEILTPVNDAGIEIMFTLSRFYGKVKEVREKSGLKKIVVSNLKEALPPFTR
                     LLFTLLKEKKDGDKLDALDSRDAWLTDLLKKYAGASKPNVDIQPDDTALFQYSGGTTG
                     VPKGAVAMHRNVVANTLQIKSWMTGLDEGKEVVLMGIPLFHVYGMVAGMNFAMATGSS
                     MVMIPNARDLKDVLENIAKYKATIFPGVPLLYNGINNHPDVKAGKYDLSSIKACISGS
                     AALMRETKEEFERLTGGKVFEGFGMSEAPTAVTCNPLNGVNKTGSIGMPLPDVDMKII
                     SLDDGETEMQLGEIGEIVVNGPQVMKGYHNMPTETANSLRQLKDGKTWLFTGDIARMD
                     EDGYFYIVDRKKELIKPGGFQVWPREVEEAIMDNPKVLEVGVGGIPDPKSGETVKAWV
                     VLKPGESMTEDELKEFCKERLAPYKVPRYVEFRTELPKTTVGKILRRELVRQHKEGGK
                     "
     gene            complement(4234..>4503)
                     /locus_tag="PLR93_05065"
     CDS             complement(4234..>4503)
                     /locus_tag="PLR93_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNH26366.1"
                     /translation="QQEELIFLGREISNQRDYSEAVAEQIDREVRKLVEDAYKQARAT
                     VKKYRKQLDAVAKKLLEVESITREEFEQIFPPPLGKKSGTPQLTA"
BASE COUNT         1179 a         1097 c         1120 g         1107 t
ORIGIN      
        1 aagaggcgtt cgatgccatg aaagcagagt ctgaggcttg gatcgtgaaa tgcttaaatt
       61 gcggacacga acgcagtatc tggagcatgg gcggcattcg gtacaaagca acaggcaact
      121 cgcgtttata tcgcatttgc ccgaattgca gtcaacgcag ttggcagacg atttcaaagc
      181 gaaaataaaa aagcgccgca gagaacctgc ggcgcttttt tatttatatg tatgttccga
      241 gtcgaacacg tgggagtgca tgttggcgat ccattgcttg ccatcttctg tgagacgcag
      301 gtataccaag gcatctttca aatacgggtt gacctgctcc cagtagaact tggcataatt
      361 cttgcgcatg tcgttcggag attgatagcc aagcttttca ttcgcgccta attcttgaaa
      421 ttcatagaac aacgccggag tcttgcggaa ataatccggg tctccgagct gaccgacaaa
      481 atctgccgct cgaaccaacc cgcccaaacc ttgggtgtct tgataaaact cacccttcgg
      541 tgaagggaag cgtgtcatct caatatacgt tgtgatcaat tcagcgttga gatgttcttc
      601 catatcttta agtagcccag acccaaagcg ttccatcacg aacaacttac tgcggttcac
      661 gtggtaaggg gtcaacgcca catcggtgcc atcttttgaa atcttaattc tttccgtccc
      721 ctcccccgtt gcaaacaatt catcggtgtc gttcttgcac acacccttta catacccaat
      781 gtcacggcag agcaccgcga tcatataacg catccaatcg cgcgcactca ctccgccttc
      841 gcgcaaatgc ttcccttcaa taatggcaag cccggcaaga gaaaccataa tggtatggtc
      901 aatatcgtga tacagcgcat ctgagttcga aatgttttcc aacgcaagcc gccccaccca
      961 agaaacaata ttgccatact ccgattgaat atcaccatag gttcggcggt acgaagtacg
     1021 caattgttcc acgaacatat caatggtcaa ttcctgccag ttgatcataa agacatccta
     1081 taagggtaaa attgaatagt gccaaaagtt taccagaaac atacaaaaag aaaacagacg
     1141 gaaatcgaat tgttatgacg aaacgccgga tctttctcaa ctggttcctg atcggactct
     1201 caatctttct tctactcaat atccttgcct cggctctttc ctctgactgt ggcttgccag
     1261 ccctctttgg gctgggtggc tgcgccgacg ccatttcacg catcggtttc ccgttcgtgt
     1321 tctttgaaca aggcggcttc gcctatcata gcagtttcaa tctacctgtt tttataattg
     1381 atattgccat tggaattggg ttcgctgtat ttcttggctc ctacaaaagt agaaaatcaa
     1441 attcctgaat gaccaaacca cccgtcatcc tcatcgttgg tcccaccgct gttgggaaaa
     1501 cggaacttgc cttgcgcctt gccgagcaaa tgaacggcga gatcgtctcg gttgattcgc
     1561 gcctgttcta ccgcggcatg gatattggca ctgccaaacc atccaaagaa gagatggcac
     1621 gtgtgcctca tcacttaatt gacatcgtta ctcccgatga gaccttgagc ttggcggtct
     1681 ttcaagagat ggcgcaaacc attattgcag gcatccacgc ccgcggacga ttgccattcc
     1741 tcgttggtgg cacagggcaa tacatccgtg cggtgactca aggttggagt ccgccagagg
     1801 tgacgccaaa tgaacagatg cgcgccgttc ttgaaacgtt acaagcggaa aaagggaaag
     1861 aatggctaca cgctaaactt caaatcctcg accctgaagc tgctcaagcc attgacttgc
     1921 gcaacgtccg ccgcacgata cgggctttgg aggtcatctt cagcacaggg cgaagattct
     1981 ctgaacaacg tggcattgga gactcgccgt atcatttaat tacgattggg ctggcacgtc
     2041 cgcgcgagga gttgtatcaa cgggtagacg aacgcattga gaaaatgttc gcagatggct
     2101 tactgaatga agtaaaaagc ctgcttgatc agggatactc ccccacattg ccaagcatgt
     2161 ctgcgatcgg gtaccgagaa tgtgtcagcg tggtacaagg ctccatgacg gaggaacaag
     2221 ccaaagtcga aatgaggcgt ttgactcgcg tctttgtgcg ccggcaggga aactggttca
     2281 aagagtcaga cccatcgatt cattggtttc atcctgcccg tgaaaattta ctcaatgaaa
     2341 ttgaagcaat gattcgtaac tctatataaa tacaggggtt gtaattgact tttaatatct
     2401 tcacgtatat acttcaaacc taggtagaga gtcatcttca gttatccaac aaaccaatgg
     2461 aggaggaagt atgagcaaca aaccgtggtt ggcgcactat gataaaggtg tgccacaaac
     2521 aatcgaatat cccaaagcgc cgttgtttca ctttttggaa gaagccgcgc gcaaataccc
     2581 agaccgtgcc tgtacgatat ttaaaggcgc ggtcatttcg tatcgtgaga tgaacgaaag
     2641 atccaatgct gtggcggctg ccttggttga gatgggtgtc aagaagggag atcgggttgg
     2701 tatttttatg cccaacctgc cgcagttcgt aagcgtgtat tttggtattc tcaaagcagg
     2761 tggcgtggtg atcgctgtga atcccacata cccggtagaa gagattctca ccccggtaaa
     2821 tgatgccggg atcgagatca tgttcacgct ttcacgtttt tatggcaagg ttaaagaagt
     2881 acgcgaaaag tcggggctta agaaaatcgt tgtttccaat ttgaaagaag ctttgccgcc
     2941 tttcacccgt ctgttgttca ctcttctaaa agaaaagaag gatggcgaca aactggatgc
     3001 gctcgatagc cgcgatgcgt ggctgacaga cttgctgaaa aagtacgctg gcgcctcaaa
     3061 accgaacgtc gatattcagc cggatgatac agcactcttc caatactccg gcggaacgac
     3121 tggcgtaccc aagggcgctg ttgccatgca ccgcaatgtg gtggcaaata cgctgcaaat
     3181 caaaagctgg atgaccgggc ttgatgaagg caaagaagtg gtcttgatgg gcattccgct
     3241 cttccatgta tatggtatgg tggcgggcat gaactttgcc atggcgaccg gttccagtat
     3301 ggtcatgatc ccgaatgcgc gtgacttaaa agatgtatta gagaatatcg ccaaatacaa
     3361 ggcgaccatc ttccccggtg tgccactttt gtataacggc atcaacaatc acccggatgt
     3421 gaaagctggc aagtatgact tgtcctccat caaggcttgt atctctggtt ctgcggcatt
     3481 gatgcgtgag accaaagaag aatttgaacg cttgaccggt ggtaaggtat tcgaagggtt
     3541 cggcatgtct gaagccccca ccgctgtgac ttgtaacccg ctcaacggcg tgaacaagac
     3601 cggctctatt ggtatgcctt tgcccgatgt agatatgaag atcatcagtt tggacgatgg
     3661 cgaaactgaa atgcaattgg gcgagatcgg cgaaatcgtt gtaaatggtc cgcaggtgat
     3721 gaaaggctat cacaacatgc cgaccgagac cgccaactcg ctgcgtcaac tgaaggatgg
     3781 aaagacctgg ctcttcaccg gtgatatcgc tcgcatggat gaagatggct acttctatat
     3841 cgttgatcgc aagaaggaac tcattaaacc gggcggcttc caagtatggc cccgtgaagt
     3901 ggaagaagcc attatggata accccaaggt cctcgaagtg ggcgtgggag gcattccaga
     3961 cccgaagagc ggtgaaacgg tcaaagcctg ggtggtgctc aagcccggtg aaagcatgac
     4021 cgaagacgaa ttgaaggaat tctgcaaaga acgactggct ccttacaaag tgccgcgcta
     4081 tgtagaattc cgcaccgagt tgcctaagac gactgttggc aaaatcttac gccgcgagtt
     4141 ggtccgccag cataaagaag gcggcaagta gttcgtaatt aataaaataa aagagcctct
     4201 caaattgaga ggctcttttt gtatgacaga tatttatgcg gtcaactgcg gggtgccgct
     4261 cttcttgcca agcggaggcg ggaagatctg ctcaaactct tcgcgggtga tggattccac
     4321 ttcgagcagt ttcttggcta ctgcatccaa ctgcttgcgg tatttcttca ccgtcgcgcg
     4381 agcttgcttg tacgcatctt ccaccaactt gcgaacttcg cggtcaatct gttccgcgac
     4441 ggcttcagag taatcacgct ggttggagat ttcgcgcccg aggaagatca attcttcctg
     4501 ctg
//
LOCUS       DAOQFT010000147         4466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10392_length_4466_cov_6.973475, whole genome shotgun sequence.
ACCESSION   DAOQFT010000147 DAOQFT010000000
VERSION     DAOQFT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10392_length_4466_cov_6.973475"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..967)
                     /locus_tag="PLR93_05070"
     CDS             complement(68..967)
                     /locus_tag="PLR93_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26367.1"
                     /translation="MNAARKSLILLVVLILSTLACGLENIQVVEAPNLEATITAQAAL
                     LQQGGQPAQDPAQQVIVVVTATPEGGVAPTEQVQAAQVLPPATGDVTVTVTTATNCRT
                     GPGQNFKIVYGMPIGQVAKVVAKNSYSGYWIIEIPGQTGQNCWLWGQYAVINGDTSTL
                     KEVVTPTSPAPTKTNTPTPTATATATTGVVFPNAPSGLTASISCAAGPGGTQTTVSGN
                     ISWVDNSTNEQVFVSSVNGVSSHTLGVNSTSDSFSTALETSALQAVAPGQQFTVSVYA
                     CSGNTAGTCGNTVTITVTGAGCP"
     gene            complement(1082..1897)
                     /locus_tag="PLR93_05075"
     CDS             complement(1082..1897)
                     /locus_tag="PLR93_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26368.1"
                     /translation="MNTIRKSLSVIFSFLCIGLLIACIPYTPPPPPPPPGVALTLTVQ
                     AIQLGFFTQTAQALQSQPIVIVVTATPDPNNLAPTTDPNAAPAATTSSAVTITVSQNT
                     NCRLGPSQDFDEIGSLGPGQTAEVVGKDTYDNYWVIKLPNNPAITCWLWGQYATVTGD
                     ISTVADSATPTAISNGAPNAPSLLDAQVKCTKLSGKDYQYKITLKWADNSSDENKFVI
                     NTSTGKTFSTPPNKTSFSFEEGLPSETNLSATITAANEKGESQPVGTGAFKCP"
     gene            complement(2159..2521)
                     /locus_tag="PLR93_05080"
     CDS             complement(2159..2521)
                     /locus_tag="PLR93_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNH26369.1"
                     /translation="MTLDLAPFEKEEYCYLTTKGRKTGTPHEIEIWFTIHENAIYLLS
                     GGMDKSDWVKNLLQDPNVTLRIAGQTFSAFASILEDKELEPMIRMAMAIKYDEWEGQD
                     PSEWARTALVVKFIPQSL"
     gene            complement(2558..3112)
                     /locus_tag="PLR93_05085"
     CDS             complement(2558..3112)
                     /locus_tag="PLR93_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26370.1"
                     /translation="MTGLPPFQQGGLDSLCGLYSIINAERIINHSTDDESQALFDGLI
                     RYLSKRGLLGKFLVGGILHPQMLLILNKVVGKKRISNVNIPWRGVPNPDLTTFWKSMQ
                     SFLDGTQGRAIILGIMGYHEHWTVIEKITNRSILLYDSALIKRLPRNFCTTVYATGQR
                     KHVLLPAQTYFLSNEVFDITQYDT"
     gene            complement(3141..3593)
                     /locus_tag="PLR93_05090"
     CDS             complement(3141..3593)
                     /locus_tag="PLR93_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNH26371.1"
                     /translation="MKFLLERSRYLTLIGIISLLIASFAAFAWGTIKTVTTTLLVIET
                     LGKDSSITIELIELIDIFLIATTILIFSASLYELFIGKLDMPEWMLAHDLYELKAKLS
                     SMIVLVMAVKYLQKLIEIKDGTELLQRGIAVAIVSAALIAFGYFGKKD"
     gene            complement(3687..>4466)
                     /locus_tag="PLR93_05095"
     CDS             complement(3687..>4466)
                     /locus_tag="PLR93_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH26372.1"
                     /translation="YAQVTAGVIIGPQWAVVIDTLALPEETLSMREFIEHELGVQVRY
                     VINTHYHADHCWGNCFFPGAVVIGHSKCRQYLVEDGAESLENAQKQNPNLRQVKIVPP
                     HITFETGEMTLRVGKKNLIISTSPGHSDDGISVLVEEDRVLFAGDAFMPLPFIVDGDA
                     DDMLNSIKKIGKMGLENIIQGHGDIILRGEIDAAVRENVNYLNNIKKAVKSGGKRKNA
                     DEYLEEIAIEDCGKSRVYLGGLAETLHRRNLRALHRQMFGE"
BASE COUNT         1057 a          979 c         1225 g         1205 t
ORIGIN      
        1 tgccctatgg atattaaaga ctcacagtcc ttgcgaattt ttcacaagga ctgagcttta
       61 ttgtttatta tgggcagcca gcgccggtta cagtgatggt gacagtgttt ccacaagtcc
      121 cggctgtgtt gccgctacag gcatagactg agaccgtaaa ttgttgacct ggagcgacag
      181 cttgaagggc gctcgtctct agggctgtgc tgaaactatc actagtcgag ttgacgccca
      241 aggtatgtga ggagacgccg ttaacactcg aaacaaagac ttgctcattg gtggagttat
      301 ctacccatga tatgtttcca ctaacggttg tctgggtacc gccgggtccg gcagcgcaac
      361 tgatgctagc ggtcaagccg gagggtgcat tggggaagac cactccggtg gtggcagtag
      421 ccgtggctgt gggggtgggg gtgttggtct ttgtcggcgc gggcgatgta ggcgtgacca
      481 cttccttcaa cgtggaggta tcgccattga tgacggcgta ttgtccccac aaccagcagt
      541 tctgcccggt ctgcccgggg atctcaatga tccaatagcc agagtatgaa ttttttgcca
      601 caacctttgc cacctgtccg atcggcatgc catagacgat cttaaaattc tgtccaggac
      661 cggtgcggca gttggttgcc gtggtgacag tgacggtcac atcgccggtg gcggggggca
      721 gcacctgagc ggcttgaacc tgttcagtgg gagcaactcc accttcgggt gtggcggtca
      781 caaccacgat gacttgttga gcaggatctt gagcaggttg cccgccttgt tgcaacaagg
      841 cggcttgtgc ggtgatggtc gcctccaggt ttggcgcttc aacgacttga atattttcca
      901 gtccgcaagc cagcgtggat aggatgagga caactagcaa gataagagat tttcgggcgg
      961 cgttcatttt taattctcct cgttcagaga taattgctga atagattttc accacagaga
     1021 aaataaaacc ttctctgtgg tgaattttct tttacttagg ctagatagaa gggatgccct
     1081 tttatgggca tttgaaggcg ccagttccta ccggctgaga ttcgcccttt tcattggcgg
     1141 cagttattgt ggcagaaagg tttgtctcgg aaggaagacc ttcttcaaaa ctgaaagagg
     1201 ttttattggg tggtgttgag aaggttttcc cggtggacgt attgattacg aatttatttt
     1261 cgtcggaaga gttatcggcc catttgaggg tgatcttata ttgataatct ttaccgctca
     1321 gtttggtgca tttcacttgg gcgtctaaaa gagatggggc attgggagca ccattggaaa
     1381 tagcagtagg cgtggcacta tctgcaacgg ttgagatatc acccgtcact gtggcatatt
     1441 gtccccataa ccagcatgtg atcgcagggt tgttgggaag tttgatgacc cagtagttgt
     1501 cgtatgtatc cttaccgact acttctgcgg tctgaccagg tcccaaagag ccaatctcgt
     1561 caaagtcttg gctcggaccg agacgacagt tggtgttctg gctgacggtg atcgtcaccg
     1621 cgctggatgt agtagccgct ggggctgcat tagggtctgt tgttggagca agattgttcg
     1681 ggtcaggcgt agccgtgact acaatgacaa tgggttgaga ttgtaatgcc tgggcggttt
     1741 gagtgaaaaa gcctagttga atggcttgta cggtgagcgt caacgcaacc ccaggcggag
     1801 gcgggggagg gggaggggta tatggaatac aagcaataag taaaccaata cataaaaagg
     1861 agaaaattac agataatgac ttacgaatag tgttcatgga gcagctcctg tacgaaatgg
     1921 gaattcccta aaatggaggt agttgtatat ttgccaaata gtcatccctt tcactccggc
     1981 gttatgaaag gctttacctg tgcggagctt ggcacatggc aaagagcatg aaataaaatt
     2041 ggcaaaacta aatgtcccca attagataat ttcccccatt ttactggact tttcatttac
     2101 gtaaggtgtt cttaagtact gtaatttact cgtaatatgt tatttatcac aaatgacttt
     2161 ataaactttg gggaatgaac ttcactacca atgctgtccg tgcccattca ctagggtctt
     2221 gcccttccca ctcatcatac ttgatcgcca ttgccatgcg gatcatgggt tctagttctt
     2281 tgtcttccaa tatactggcg aaggcagaga aggtttgacc cgcaatgcga agagtgacgt
     2341 tggggtcttg cagcaagttt ttgacccaat ccgatttgtc catgccgcca gagagaaggt
     2401 agatcgcgtt ctcatggatg gtgaaccaga tttcgatctc atgaggcgtt ccggttttcc
     2461 gtccctttgt ggtgaggtaa cagtattctt ctttttcaaa tggagcaagg tctagggtca
     2521 tatatgtctc caaaatcatt cagtaggtgg gctaagtcta ggtatcgtat tgtgtaatat
     2581 caaaaacttc gttggagaga aaataggttt gtgccggtaa tagtacgtgc ttgcgttgcc
     2641 ctgtggcgta gacggtggtg cagaagttgc gtggtaagcg tttgatgagt gccgagtcgt
     2701 acaacagaat agaacggttg gtaatttttt cgatgacggt ccaatgctcg tgatacccca
     2761 tgatgccaag gatgatggca cgaccttggg tgccatctaa aaaggattgc atcgacttcc
     2821 agaatgtggt gaggtctggg ttgggaacgc cgcgccaggg aatatttacg ttggagatac
     2881 gcttctttcc tacgaccttg ttcagaatca gcaacatctg tgggtggagg attccgccga
     2941 cgaggaattt tccaagtaac ccgcgcttgg aaaggtagcg gatcagtccg tcaaacaagg
     3001 cttgcgattc atcatcggtg gaatggttaa tgatgcgttc ggcgttgata atactgtaca
     3061 agccgcaaag tgagtccaag ccgccttgtt gaaagggcgg aagtcccgtc attaagcggc
     3121 ttgaccttct catggtgcca tcagtccttt ttgccgaaat atccgaaggc gatcagcgcc
     3181 gcagagacta tggcaacggc aatgccgcgt tggaggagtt cagttccatc tttgatttcg
     3241 atcagttttt gcaaatactt gactgccatt accagcacga tcatgctgct gagtttggct
     3301 ttgagttcgt acaggtcatg tgccaacatc cattcgggca tgtctaattt gccgatgaag
     3361 agttcgtaca ggctggcaga gaagatcaga atggtggtgg cgatcaggaa aatgtcgatt
     3421 agttcgatca actcgatggt gatggaactg tctttgccga gggtttcgat tactagcagc
     3481 gtagtagtca ctgttttgat ggtgccccag gcaaaggcgg cgaaagaagc gatcaacaga
     3541 gagatgatgc cgatcaaagt taggtagcgt gaacgttcca gtaaaaattt catgttgtta
     3601 atcctcttgt cataatgtat aaggctctag ccactaacaa agatgttgca gacccggctt
     3661 gcatctgcaa catctagtgt attcgtttac tctccgaaca tttggcggtg taaggctcgc
     3721 aggttgcgcc tgtgcagggt ctctgccagc ccgccgagat agacacggct cttgccacaa
     3781 tcttcaatgg cgatctcttc gagatattca tcggcattct tgcgtttgcc acccgacttg
     3841 actgcttttt tgatattgtt gaggtagttg acattctcac gtacggcggc gtcgatctca
     3901 cctcgcagga tgatatcgcc atgaccttgg atgatatttt ccaagcccat cttgccgatc
     3961 ttcttaatgg agttcaacat gtcatcggca tcgccatcta caatgaaggg caatggcatg
     4021 aaagcatcac cggcgaagag cacacggtct tcttcgacga ggacggaaat gccatcgtca
     4081 ctgtgaccgg gggaagtgga gatgatgaga tttttcttac ccacacgtag ggtcatttca
     4141 ccggtttcaa atgtgatgtg cggcggcacg atcttaacct gacgcaggtt ggggttttgt
     4201 ttttgagcat tctccaatga ttcggcgcca tcttccacca gatattggcg gcatttcgaa
     4261 tgaccgatca caacagcgcc ggggaagaaa cagttacccc agcagtggtc ggcgtgataa
     4321 tgggtgttga tcacataacg gacctgtacg cccaattcgt gctcgataaa ttcgcgcatg
     4381 gagagggtct cttcggggag agcgagggta tcgatgacca ctgcccattg cggaccgata
     4441 atgacacccg cagtgacctg cgcgta
//
LOCUS       DAOQFT010000148         4462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10408_length_4462_cov_7.359428, whole genome shotgun sequence.
ACCESSION   DAOQFT010000148 DAOQFT010000000
VERSION     DAOQFT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10408_length_4462_cov_7.359428"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..580)
                     /locus_tag="PLR93_05100"
     CDS             complement(8..580)
                     /locus_tag="PLR93_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH26373.1"
                     /translation="MTTLMNMEIKELDEEDLARKAISDADAFAELYRQHVTRVYRYHM
                     AHIGNVKDAEDLTSQTFMAAMESIRSFRGDGSFAAWVMGIASKKRLMYFRGNRHEVPL
                     EAAEHYPNPSLPTDKAAMQRIRLQSISHALKQISSERSEAIVLIYFGGLSYAEAAGVM
                     RKNEASVKMLVSRGLQDLRERTSLRMEVEE"
     gene            complement(685..1161)
                     /locus_tag="PLR93_05105"
     CDS             complement(685..1161)
                     /locus_tag="PLR93_05105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH26374.1"
                     /translation="MSKIKQPNSRHCFICGVENPVGLHLHFYETEPGVIETEYTAPDH
                     FEGFPGVLHGGIVAAIIDEVSGRAYMGNDPANPRFMFTGKLEIKYRKNVPIGKLLRIV
                     GKVMENKGRIAKAWGGIYDAETNELLAEGTGMHINVPSDQFDMSKLEELGWKIYPD"
     gene            complement(1161..1853)
                     /locus_tag="PLR93_05110"
     CDS             complement(1161..1853)
                     /locus_tag="PLR93_05110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNH26375.1"
                     /translation="MTQLTGQERAQYVQSMFSKIAHRYDLMNRLMTGGQDIRWRREVI
                     SLTKIRPHGSILDLGTGTGDLAREALTQYPKAQVTAADFTLEMMRVGKKNGDLNFSTA
                     DALNLPFKGKNFDAVVSGFLMRNVTDVQQALREQHRVLKPGGRIVILDTTRPKKNLLS
                     PFIKFHMHVIIPMVGGMLSGMRDAYEYLPDTTENFLTAEELSVRMVAVGFRRVNFKRL
                     MLGTIAIHWGEK"
     gene            1914..3176
                     /locus_tag="PLR93_05115"
     CDS             1914..3176
                     /locus_tag="PLR93_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH26376.1"
                     /translation="MSTTSYPFARESQLALDQARRIAEGNKLPFVDSLMLLLTLLQLK
                     NSRVQALLKSLNVRVDNLTARIVATIKLEAKEILQVSEGKPVLSSECETVLALASAEM
                     QLAGQNSISEFSLLLGMLQNTESKAGQILSQYAVAVDQAREKIKIFKETPVSQSNILR
                     KDGFLRRAFRNGISPIFISLVLFTIAMAAFLWLGIGNNPRAFLIAFIIGGWIISVALH
                     EFGHAITAFWGGDESVEDKGYLTLNPLKYSNPVLSVIIPVMMLLLGGIGFPGGAVYIN
                     IFALRKPKYRSLVSAAGPLANLLVAILLAIPFANFPFFFFFIYAPQKFLMALAFLAFL
                     QITSIIINLLPIPGLDGFGILEPFLSREIVGVASFIRPFGFLILYLFVFIDSPISNMF
                     WQSIWAITNLLSPSLAYYANEGLKLFLN"
     gene            3184..4083
                     /locus_tag="PLR93_05120"
     CDS             3184..4083
                     /locus_tag="PLR93_05120"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNH26377.1"
                     /translation="MAETNKPNMFQIWWLAIRPRTLPAAAAGVAMGSALAWHDGSFQL
                     LPALAALLVALLLQVGSNVANDVFDFERGADTAERLGPVRVTQAGYLTPSQVKTGMVV
                     IFAFAAVLGLYLATLRGWTVIWLGAAAILSAVAYTGGPYPLGYHGLGDVFVFLFFGLA
                     AVAGTYFVQVGYVSPAAWWMAVPVGLIVTAILVVNNLRDLENDRKAGKRTLAVRFGEE
                     GAKAEYLLLMLIAYLIVPVLVWMNIIPIGALLAWLSLPIAIRTLRTVFTQKGKPLNVA
                     LAGTGQTAFLFSLFFWVGLLFSN"
     gene            complement(4076..>4462)
                     /locus_tag="PLR93_05125"
     CDS             complement(4076..>4462)
                     /locus_tag="PLR93_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH26378.1"
                     /translation="IMFLILFFQIIVLVRDQASGIAPQLLQSLQSLGAGRRALFRFVY
                     LPASLPAILTALRQSIGTAVAVLYITELSATKYGLGYYIYYKGSTLLDYPSMYAGVIA
                     MSLLGLGMYFTVDWLERKWCPWKFVS"
BASE COUNT         1040 a         1047 c         1068 g         1307 t
ORIGIN      
        1 aattcgttca ttcttccacc tccattctga gggaggttcg ctcgcgcagg tcttgcagcc
       61 cgcgtgagac gagcattttg acagacgcct cattttttct catcacgcct gcggcttcgg
      121 cgtagcttaa tccgccaaag tagataagca cgatcgcttc agagcgttca cttgaaattt
      181 gcttgagcgc atgagaaata gactggagcc ggatgcgttg cattgcggct ttatcggtag
      241 gtaagctcgg gttgggataa tgctccgccg cctctaacgg gacctcatgc ctgttcccgc
      301 gaaaatacat aagtcttttc tttgaagcga ttcccatcac ccacgccgca aacgagccat
      361 ctcctcgaaa cgagcggata ctttccatgg ccgccatgaa ggtctgcgag gttaagtctt
      421 cggcatcttt tacgttgccg atatgtgcca tgtgatagcg gtatacccgc gtcacatgct
      481 gacggtaaag ctctgcaaaa gcatctgcat ctgagatggc tttccgtgct aggtcttctt
      541 cgtctagttc tttgatttcc atattcatta gggtggtcat atacctcctg tgagtagtgc
      601 gcacatagat atgttgcagg aggttgaaaa aagtaacagg caaaataaaa ggtgacagtc
      661 actttataag cgactgtcac ctggctagtc agggtatatc ttccaaccta actcttcaag
      721 cttgctcata tcgaactggt cacttggcac attgatgtgc atgcctgtcc cttcggcaag
      781 cagttcattc gtctctgcat cgtaaattcc gccccaggct ttggcaattc gccccttatt
      841 ctccattact ttaccaacga tcctgagcaa tttgccaatc ggcacattct tgcggtattt
      901 gatctctaac ttgcccgtaa acataaaacg cggattcgcc ggatcattgc ccatgtatgc
      961 ccgcccagag acttcgtcta tgatcgcggc gacgatgccc ccatgcaaca caccagggaa
     1021 gccttcaaaa tggtcgggcg cagtgtattc ggtttcgatg actcccggtt cggtttcata
     1081 aaaatggagg tgtaagccga caggattttc aactccacaa ataaagcaat gacgtgaatt
     1141 tggttgtttg attttagaca ttatttttct ccccagtgaa tggcaattgt gccaagcatc
     1201 aggcgtttga aattaactct tctgaagcca acagccacca tacgaacgga cagttcttcg
     1261 gcggtcaaaa agttttctgt tgtatcaggt aaatattcat aagcatcgcg catgccagaa
     1321 agcattccac ccaccatggg gatgatgaca tgcatatgaa atttgatgaa cggtgaaagt
     1381 agatttttct ttgggcgtgt cgtatcaaga atgacgatac gtccacccgg tttgagcaca
     1441 cggtgttgct ctctcaacgc ttgttggacg tcggtcacat tgcgcatgag aaaaccagag
     1501 acaaccgcgt cgaagttttt tcctttgaat ggcaaattca aagcatcggc agtagagaag
     1561 ttcaaatctc cattcttttt gccgacacgc atcatttcaa gcgtaaaatc tgctgcggtg
     1621 acttgggctt tggggtactg tgtcagcgct tcgcgagcta aatcaccggt acccgttccg
     1681 aggtccagaa tagagccgtg cgggcggatc ttcgtcaggc tgatgacttc tctccgccaa
     1741 cggatgtctt gcccgcctgt catcagtcgg ttcatcagat cgtagcgatg ggcgatctta
     1801 ctgaacatgg attgtacata ctgggcacgt tcctgcccgg ttaattgggt cataaattct
     1861 cctgaaaaac gaattataca cagaaggttt ttgacgcacg gtataatcgc aaaatgtcaa
     1921 ctacatcgta tccatttgcc cgggagagcc agcttgcgct tgaccaagct cgccgcattg
     1981 cagaggggaa taaacttcct tttgtagatt ctttgatgct tttgcttact ctgttgcaac
     2041 tcaaaaattc acgtgtgcaa gccttgttga aatctctcaa tgtcagagtg gataatctta
     2101 ccgcacgcat tgtggcaact atcaaactgg aggcgaaaga gatccttcaa gtttctgagg
     2161 ggaaaccggt cttatcttct gaatgtgaaa ctgttttagc attggcttct gccgagatgc
     2221 aactggcggg acaaaactcg attagtgaat ttagtttgct gttgggcatg ttacaaaaca
     2281 cagaaagcaa agcaggtcaa atattatctc agtatgcagt tgcagtagac caagcccgtg
     2341 agaaaattaa gatattcaaa gaaacccctg tttcacaaag taatattttg cgcaaagatg
     2401 ggtttctgcg cagagcgttt cgaaatggca ttagcccgat ctttattagt ttggtgttgt
     2461 ttacgattgc catggcggct ttcttgtggc tgggcattgg gaataatccc agagctttct
     2521 tgattgcgtt cattatcggc gggtggatta tttctgttgc attgcatgag tttgggcacg
     2581 ccatcacagc cttttggggt ggcgatgaaa gtgttgaaga taaaggctat ctcaccctca
     2641 acccgcttaa atattcgaac ccggtgttga gcgttatcat tccagttatg atgttgctat
     2701 taggtgggat tggttttccg ggtggagcag tgtatatcaa tatcttcgca ttgcgcaaac
     2761 caaaataccg cagcctagtt tctgcggctg gacctttagc aaatttattg gtcgctatat
     2821 tgttagcgat cccattcgct aacttcccat tctttttctt ctttatttat gcccctcaaa
     2881 agtttttgat ggcactggct tttctggcgt ttttgcagat tacgtctatc atcatcaatc
     2941 tattgcccat ccccggcttg gatggcttcg gtattcttga gccgttctta tcacgagaaa
     3001 tcgttggtgt tgccagtttt attcgcccgt ttggcttttt gattttgtat ctctttgtgt
     3061 tcatcgattc gcccatttca aatatgtttt ggcaaagtat ttgggcaatc acgaatttac
     3121 tcagcccaag ccttgcctat tatgccaacg agggtttgaa actctttttg aattaacaac
     3181 gttatggctg aaacaaacaa accgaacatg tttcaaattt ggtggctcgc catccgtccg
     3241 cgcaccttgc ctgcggcggc ggcaggagtt gcaatgggca gtgctttggc atggcatgac
     3301 ggttcatttc aattgttgcc cgcgttggcg gctttgttgg tggcattgtt gttgcaggtc
     3361 ggtagtaatg ttgcaaatga tgtgtttgac tttgaacgcg gtgccgatac ggctgaacgg
     3421 cttggtcccg tgcgtgtgac tcaggctgga tatctgactc catcgcaagt gaagacgggc
     3481 atggtggtaa tttttgcttt tgcagcagta ttgggtttat atctcgccac gcttcgcggc
     3541 tggaccgtga tctggctcgg cgctgctgcc attctctccg ctgttgccta caccggcgga
     3601 ccttaccctc ttggctatca tggcttgggc gatgtttttg tctttctctt tttcggtctc
     3661 gctgctgtag cagggacata ttttgttcaa gttggctacg tcagccctgc cgcttggtgg
     3721 atggcagttc cggttggttt gatcgtgact gcgattttgg tcgtcaacaa tttgcgcgac
     3781 ctcgaaaacg accgtaaagc aggcaaacgc acattagctg tccgctttgg agaagagggc
     3841 gccaaggcag aatatctgct cttgatgttg attgcttatt tgatcgtgcc agttttggtg
     3901 tggatgaaca ttattcccat tggtgcatta cttgcatggc tatctctccc gattgccatt
     3961 cgtactttga gaacggtttt tactcaaaaa ggaaagccac tcaatgtggc tttagcaggc
     4021 actgggcaaa cagccttttt gttcagcctt ttcttttggg ttgggttact tttttctaac
     4081 tgacaaactt ccacgggcac cattttcgct ccagccaatc gactgtgaag tacatgccaa
     4141 gcccaagcaa gctcatggcg atcacacccg catacattga cgggtaatcc agtaacgtgc
     4201 tgcctttgta atagatgtaa tatcccaacc catattttgt tgctgaaagt tctgtgatgt
     4261 acaacaccgc caccgccgtg ccaatggatt ggcgtagtgc agttagaatg gctggcaaac
     4321 tagcgggtaa ataaacgaaa cggaagagcg cgcgcctgcc cgcaccgaga ctttgcaggc
     4381 tttgaagcaa ttgcggcgca ataccacttg cctgatcgcg gacaagtaca atgatctgaa
     4441 agaacagaat caagaacata at
//
LOCUS       DAOQFT010000149         4455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10430_length_4455_cov_6.009773, whole genome shotgun sequence.
ACCESSION   DAOQFT010000149 DAOQFT010000000
VERSION     DAOQFT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10430_length_4455_cov_6.009773"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLR93_05130"
     CDS             <1..1035
                     /locus_tag="PLR93_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH26379.1"
                     /translation="PFGTEAYRKAVIEKYWKLDASSGWGPANVLGTAGGRDALNKAYQ
                     AMLALGYGRQGDLIMVSRVPWISYNWGPYGVGANVLWTPGDPAQGWAYSEEAIRESVK
                     FAESKGRKIAGIVITNPDNPTGMTIAVEKQVSLAKAALEAGVAFVLFDWMYHYVTDES
                     PMDLNSFLKAFTPEERKRIMFLDGITKSLGGSNIRNCHLIADEAVIKFITARASHGVI
                     PSFFSLAVAMAAYEMGFNEAAKTIIEPTNASRVALKKLLNESGLQHIIGKGYYAFMNV
                     GEFIKAKGWADTEPLGQYLAENHGVAVVPGAFFSPFGGEWIRFSYATPVERTEGAFKR
                     LIEGLNELKK"
     gene            1121..3913
                     /locus_tag="PLR93_05135"
     CDS             1121..3913
                     /locus_tag="PLR93_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH26380.1"
                     /translation="MPQQFAEKYLLALEESIKEKPKGGLAGFEQEWNLLDNDLRPLLT
                     VGAGPSQHSFVDYLLAECVPTWQKQFSQLEVFHWMVEWATRPYYSPRGAIYEARLMEA
                     SLMNALHRAGLSFGERLHYWHGNLLFLTDIGHQSIPGNWSLAKRRYLEKCVDLYGDTL
                     ATTGIHTNISLPDPLFAWDFMHLSASERGDKHLDDYKSEFYITATRLLRAFASLFIAT
                     SASTPMQAQVKDGRAAVILTEFDSIRNLTFPNPREVDLPDLYRSYNDYLQISYDLVRR
                     GVRFGNNNWTPVRARSFSEPVERIISTTSEQLESLYARGLFAAGESTPPDEMAKQVER
                     QNLMARINLPMGRVEIRVDEGGHSLDLDVANMTFRYLLMLKIYADPKFARGFRYDSED
                     ITRARTNENLAAKHGLRAEIENPLTGKPIVVRDFLKWTLNEIKPMAQALNMWNDLSPL
                     VEMAEGGKNTAEKIRARLKMEIGNSDEVPMDLLKEFLYEHEAQVKSDVERVCADYNAL
                     GSDASKIAEYVQRGREDARQIPNAPIQYRAKTHAAVELSYPNKTAEILDLAQQLIRIP
                     SVTACPEERLDEVHRAGSLIDDYLRNAGLDVKYYDGKYPAVYATFPGASKPDPILLTG
                     HFDVVEPEPDDSQFNPRIEGDYLHGRGAADMKTVVATYLVWMKDQMKAGAPYPNIALM
                     LVGNEENGEAEEWGTPHVLKDLNLTPSLFVAGERTGEKGNEIFGEICIENRGVMRFDV
                     IARGAKAHSGVAGTGDLSEKLIAARTALNEMFSKHLTLKAADGWQSQAKFPFISVGTP
                     GVYNVTAAEGILGVEIRPIPQDDVEGLKSKVEDYCAENGLEVKFTVMENGVACDPNNP
                     ALKALIEAVRQASGGEEPKIGRKLPGTSARFAPGGQAVVWGQSGVGPHAKNEAHFIPS
                     IEPYYKSLNELAKNWK"
     gene            complement(3952..>4455)
                     /locus_tag="PLR93_05140"
     CDS             complement(3952..>4455)
                     /locus_tag="PLR93_05140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNH26381.1"
                     /translation="EALREVPIKAIYVSPLERAMETAAPIAESHKLVIQQEPDLMDTD
                     IGKWQGKSWKLLALKKEWKIVQNAPSRFRFPEGESFPECQLRIANVLERVIKKHNKPQ
                     DIVAVVFHADPIKLAVAHFLGMPLDHFQRLGCDTGSLTAIHAGEAGAHLVKMNYRPPF
                     DFLPKKK"
BASE COUNT         1107 a         1164 c         1087 g         1097 t
ORIGIN      
        1 ccgttcggca ccgaagccta tcgcaaggcg gtcattgaaa aatattggaa actagatgcc
       61 tcttctggct ggggacctgc caatgtgctc ggcaccgccg gcggacgtga tgccctcaat
      121 aaagcttatc aagccatgct tgccctcggc tatggtcgcc aaggtgacct tattatggtc
      181 tcacgtgtgc cgtggatttc atataactgg ggtccatacg gtgtgggcgc caatgtcttg
      241 tggactcccg gcgacccggc gcaaggttgg gcatattcag aagaagcgat tcgtgaaagc
      301 gtcaaatttg ccgaatcaaa aggacgcaag atcgcgggca ttgtcattac caaccctgat
      361 aaccctaccg gtatgaccat tgctgttgaa aagcaggtct cgcttgccaa ggctgccctt
      421 gaagcaggcg ttgccttcgt actcttcgat tggatgtatc actacgtcac cgatgaatcg
      481 ccaatggatt tgaattcttt ccttaaggca ttcacccccg aagagcgcaa acgcatcatg
      541 ttcctggacg gaatcaccaa atccttgggc ggttcaaaca tccgcaactg tcacctcatt
      601 gcagatgaag ccgttatcaa gttcatcacc gctcgtgctt cccatggcgt catcccttcg
      661 ttcttctcgc ttgcggttgc catggctgcc tacgaaatgg gtttcaacga agctgccaag
      721 accatcattg aaccgaccaa tgccagccga gttgccctca agaaacttct caacgaaagc
      781 ggacttcaac acattatcgg taaaggctat tatgccttta tgaacgtggg cgaatttatc
      841 aaagccaaag gctgggcaga tactgaaccg ctgggtcaat accttgctga aaatcacggc
      901 gttgcggtcg ttccgggtgc atttttctca cccttcggcg gcgaatggat ccgcttctcg
      961 tatgctacgc ccgttgagcg taccgaaggc gcattcaaac gcttgataga gggcttgaat
     1021 gaattaaaga agtagtgaca gaaagtagaa agtgatgttc gtcctcactt tctactttct
     1081 gttttatagc cattattcca atttatctta ggaaatatcc atgccacaac aatttgccga
     1141 aaaatattta cttgcccttg aagaatccat taaggaaaaa ccaaaaggcg gtctcgctgg
     1201 gtttgaacag gaatggaatc tgctcgataa cgatctccgt ccgctgttaa ctgtcggtgc
     1261 cggacccagc cagcactcct ttgtggatta tctgcttgca gaatgcgtcc ctacatggca
     1321 gaagcaattc agtcagcttg aagtcttcca ctggatggtg gagtgggcaa cccgcccgta
     1381 ttattctcca cgcggggcga tctatgaagc ccgtctgatg gaagcctcat tgatgaatgc
     1441 tttgcatcgc gccgggctga gctttggcga acggctgcat tattggcatg gtaatttgct
     1501 cttccttacc gatattggtc accaatcgat tcccggcaat tggtctttag ccaagcgccg
     1561 ctatctcgaa aaatgcgttg acctctatgg tgatactctc gctaccacag gcattcacac
     1621 caatatctcg ttgccagacc cactcttcgc atgggatttc atgcatctct ctgccagcga
     1681 acgcggcgat aaacatttgg atgattacaa gtctgagttt tatatcaccg ccacacgcct
     1741 cttacgtgct tttgcttctc tcttcattgc gacgagcgct tctacgccga tgcaggctca
     1801 ggtcaaagac ggacgcgccg cagtcatcct caccgagttc gactcgatcc gtaatctcac
     1861 cttcccgaat ccgcgcgaag tggacttgcc cgacctctac cgttcttata acgattactt
     1921 gcaaatctcc tatgaccttg tccgccgtgg cgtgcgcttc ggtaacaaca actggacacc
     1981 cgtccgtgcc cgcagtttct ctgagccggt agagcgcatt atttccacga cgagcgaaca
     2041 acttgagtct ctctatgctc gcggactctt cgccgcaggc gaatccacgc cgccagatga
     2101 aatggcaaaa caggttgaac gtcagaacct gatggctcgt atcaacctcc caatgggacg
     2161 tgtcgaaatc cgcgtagatg aaggtggtca tagcctcgac cttgatgttg ccaacatgac
     2221 tttccgttat ttgctcatgt taaagatcta tgcagacccg aaatttgcgc gtggcttccg
     2281 ttacgacagt gaagatatta ctcgtgcgcg caccaatgaa aatttggcag ccaaacatgg
     2341 cttgcgcgct gaaattgaaa atccactcac gggcaaacct atcgttgtac gagattttct
     2401 caagtggacg ttgaacgaga tcaaacccat ggcacaagcc ttgaatatgt ggaatgactt
     2461 gagtccgctt gtggaaatgg ctgaaggcgg caaaaatact gcagaaaaga ttcgtgcccg
     2521 tctcaagatg gaaattggaa atagcgacga agtccccatg gacttgctca aggaattttt
     2581 gtatgagcat gaggcacaag ttaaatccga tgtcgagcgt gtctgcgcag attacaatgc
     2641 cttgggtagt gatgcgtcca aaattgccga atatgtccaa cgtgggcgcg aagatgctcg
     2701 tcaaattccg aatgccccca ttcaataccg cgcaaagaca cacgctgcgg ttgaactttc
     2761 ctatccgaac aagactgccg aaattcttga tcttgcccag caattgattc gcattccatc
     2821 ggtcactgcc tgccctgagg aacgtctgga tgaagtccac cgtgccgggt ctttgatcga
     2881 cgattattta cgcaatgcgg gtttggatgt gaaatactat gacgggaaat accccgctgt
     2941 ttatgccacc ttccccggcg cctcaaaacc ggacccgatt ctgctcaccg gtcactttga
     3001 cgtggtcgaa cccgaacccg atgactccca attcaatccg cgcattgagg gagattacct
     3061 ccacgggcgc ggtgcagcgg atatgaaaac cgttgttgct acctatttgg tctggatgaa
     3121 agaccaaatg aaagcaggtg ctccatatcc aaatattgcc ttaatgttgg tcgggaacga
     3181 agaaaatggc gaagctgagg aatggggtac accccatgtg ctgaaagatt taaacctcac
     3241 accttcgttg tttgttgctg gcgaacgcac tggcgaaaaa ggcaatgaaa tttttggtga
     3301 aatctgcatc gaaaatcgtg gtgtaatgcg ttttgacgtg atcgcccgcg gcgccaaagc
     3361 acatagcggt gtagctggta ccggcgactt aagcgaaaag ctgattgctg ctcgaaccgc
     3421 cttgaacgag atgttctcca aacatctcac acttaaagcc gcagacggct ggcagtcaca
     3481 agccaagttt ccgttcatta gtgtaggcac acccggtgta tataacgtca cggcagcgga
     3541 agggattcta ggcgtggaga ttcgccccat tcctcaagac gatgttgaag gcttaaagtc
     3601 aaaagtggaa gactactgcg cagaaaatgg gcttgaagtt aaattcaccg ttatggaaaa
     3661 tggtgttgcc tgtgacccga ataatccggc gttgaaagca ctcatcgaag cagtcaggca
     3721 ggcatcgggt ggggaggagc cgaagatcgg tcgtaaactc cctggtacca gcgcacgctt
     3781 cgcgcccggc ggtcaagccg tggtgtgggg gcaatccggt gttggtccac atgcgaagaa
     3841 cgaggcgcac ttcatcccaa gcattgagcc gtattacaag tcgctcaatg agttggcgaa
     3901 aaactggaag tagaagtaaa agaaggacga gagcaatctc gtccttcttt tttatttttt
     3961 cttcggcaga aaatcaaacg gcggacggta attcattttc accaaatgtg cgcctgcttc
     4021 tccagcatgg atcgcggtca gagaaccggt atcgcatccc agccgttgaa agtgatcgag
     4081 tggcataccg agaaaatgtg ccacagccag tttgattggg tctgcatgaa agaccaccgc
     4141 cacaatatcc tgcggcttgt tatgcttttt aattacccgt tccaatacat tggcaatccg
     4201 caactggcat tctggaaaag actcgccttc aggaaaacga aaccgcgacg gtgcattctg
     4261 tacgatcttc cactcttttt tcaacgctaa cagcttccac gactttccct gccatttgcc
     4321 gatgtctgta tccatcaggt caggctcttg ctgaatcaca agtttatgcg actcagcaat
     4381 tggcgcggca gtttccatcg ccctctcaag cggactcaca taaatagcct tgatcggcac
     4441 ctcacgcagc gcctc
//
LOCUS       DAOQFT010000150         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10456_length_4447_cov_6.306011, whole genome shotgun sequence.
ACCESSION   DAOQFT010000150 DAOQFT010000000
VERSION     DAOQFT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10456_length_4447_cov_6.306011"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLR93_05145"
     CDS             <1..850
                     /locus_tag="PLR93_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH26382.1"
                     /translation="HEIIGVDRGKLVNAPFKILRYDLLEKNAVESILDSARPDWVVNC
                     AALADLDICEDDPDLAKQLNTNLPRMLARACKLRNIPLAHISTDAVFDGHKDGFYSEE
                     DAPSPTGVYAKTKLDGEWAVLTEYPKAIVARVNFYGWSLTGRRSLAEFFYNNLTHNKS
                     MSGFTDVIFCPMLVNDTARTLVKMLKRELKGLYHLVGPQAMSKYQFGVEIARRFNMRE
                     SDITPKSVNFSGLIAKRSNNLWLSTRKLSADLGEDLPNFSTGLDEFYTQYEQGYPQKI
                     RGYQQP"
     gene            1181..1636
                     /locus_tag="PLR93_05150"
     CDS             1181..1636
                     /locus_tag="PLR93_05150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNH26383.1"
                     /translation="MRFPNGFVIDKYHLLDFEREAVVALMENADGEVIFALIPRYPSN
                     TVEWELPAGGMEVGEEILEAAKRELLEETGYSSTDHKLIYTYYPMNGNANQVFHIVFC
                     KAGERVQDFDLNEVADVRWFKREEIKQLLKDQKIKDGFSLTALLLWLQN"
     gene            1669..2736
                     /locus_tag="PLR93_05155"
     CDS             1669..2736
                     /locus_tag="PLR93_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNH26384.1"
                     /translation="MEFKINNHTIGLNHPTYFIADIAANHDGDLDRAKLLIRLAKEAG
                     ADAAKFQHFQAPKIVSEYGFTHMNSKVSHQANWKRSVTEVYAAASVSQSWTPILKEEC
                     DKVGIDFFTSPYDYDSIEHVNPYIPAFKAGSGEIDWIEALVHMASKGKPMIIACGAAD
                     IADVQRAVQAILKVNKQLCLMQCNTNYTANPNNYDHLHLNTLKTFAAMYPDVILGLSD
                     HTHSVAPVLGAITLGARMIERHFTDSNDREGPDHKFAMNPANWAKMVEETRLLERSLG
                     SSDKFIAANEQETKVVQRRCLRAARDIKAGETFTRDMIDVLRPATPGAVKPHEIENVI
                     GTKALVDMPKGKELRWVDLGA"
     gene            2791..3177
                     /locus_tag="PLR93_05160"
     CDS             2791..3177
                     /locus_tag="PLR93_05160"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNH26385.1"
                     /translation="MTKFEDLIAWQEARTLVKWVYKITSDGAFSKDYGMRDQIQRAAV
                     SVMTNIAEGFDCESSAEFARFLGIARRSAVEVQSLLYAALDVDHIKEDVFKLHYEQAK
                     KCKALIGGLKQSVLKNPRRNNTPSRA"
     gene            3202..4284
                     /locus_tag="PLR93_05165"
     CDS             3202..4284
                     /locus_tag="PLR93_05165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH26386.1"
                     /translation="MKLIYFSLGYSTHDYRFLKAISDGGHDVHFVQLEGNRRQVESRS
                     VPENVNQVIWKGGREAFTWNKLPALVMDFKRLLRDLKPDLVHAGPIQTCAFIAVLAGA
                     RPLLTMSWGFDLMDDVHKGWMWEFATKYTLKRSTFFTSDANVTKDKAVAYGMNPEKTI
                     VFPWGVDLDHFTKKEEIDSKEGFVLFCNRSWETRYGVDVLARAFVKVASQREDIRLLL
                     LGGGSQGAYLRKIFQSGGVDEYVTYGGQISQTDLPRWYHMADLYISPSHVDGSSVSLM
                     EALACGLPCLVSDIPANKEWVFENQNGWLFRDGDVDHLAEKILAAMNQREKLPEIGRA
                     SRSEAEKRADWKKNAAALMEVYRSLK"
BASE COUNT         1170 a         1043 c         1106 g         1128 t
ORIGIN      
        1 acatgagatt attggcgtag atcgcggtaa gttggtcaac gcgccattta agatattgcg
       61 atatgacttg cttgaaaaga atgctgtcga atcgatcctc gactctgcca gacccgattg
      121 ggtggtgaat tgcgcggctc ttgcggacct cgatatttgt gaagacgacc cagaccttgc
      181 caaacagttg aacacaaacc tgcctcgaat gctggctcgt gcctgcaaat tgcgcaatat
      241 ccctttggcg catatctcta ccgatgcggt ttttgatggg cacaaagatg ggttctacag
      301 cgaagaagat gctcccagcc ctaccggtgt atatgccaag acaaagctgg atggcgaatg
      361 ggctgtgttg acggaatacc ccaaagccat tgttgctcgt gtcaactttt acggctggag
      421 cttaaccggg cggcgcagcc ttgcagaatt cttctacaac aacctcactc acaacaaaag
      481 catgagcggt tttaccgatg tcatcttctg ccccatgctg gtgaatgata ctgcccgcac
      541 cctcgttaag atgttgaagc gtgagttgaa aggcttgtat cacctcgtgg gtccacaggc
      601 gatgagcaaa tatcagtttg gggtagaaat cgcgcgccgc ttcaatatgc gcgaaagtga
      661 cattacccca aaatctgtca acttttccgg attaattgcc aaacgttcca acaatttatg
      721 gctttctacc cgcaagttat ccgcagattt gggtgaggat ctccccaact tttccacagg
      781 tttggacgag ttttacacac aatacgaaca aggttatcca cagaaaattc gtggttatca
      841 acaaccttag ccacagtttg agccgatttt tggcatcttt ttctgccact tatccacaga
      901 tttccccact taaattgggg tttttccaca ggaatcaggc agatcttgcc aaaatcccga
      961 ggttttgaac cgagttatcc acagaatcca cacacttatc cacaggctgg agtacgttta
     1021 cacacaataa cccagctctg tgacgtgttt atccactgtt ttacccacag cccttgttga
     1081 taaaacagtt acgaggagat tttctttcac catgaagcca gaaagattgg caagagaggt
     1141 cgtgtaccaa agtagatggg ttaatttata caaggacact gtgagatttc cgaatggttt
     1201 tgtgatcgat aaataccatt tattggattt cgaacgtgaa gctgtggttg cccttatgga
     1261 aaatgcggac ggggaagtaa tttttgccct gatccctcgc tacccaagta atacggttga
     1321 atgggaactc cccgcaggcg gcatggaagt tggtgaagag attctcgaag ccgccaaacg
     1381 cgagttattg gaagaaacag ggtatagctc aaccgatcac aaattgatct acacctatta
     1441 ccccatgaac gggaatgcca atcaggtctt tcatattgta ttttgcaaag ccggtgaacg
     1501 cgtgcaagat ttcgacttga atgaagtggc tgatgttcgc tggtttaagc gggaagagat
     1561 caaacagttg ttgaaagacc aaaagatcaa ggatggcttt tcactaactg cattgctttt
     1621 atggctgcaa aactaaataa tctttcatcg aaataaagga gagacgacat ggaattcaaa
     1681 atcaataacc atactatcgg attgaatcac ccgacctact tcatcgctga tatcgcagca
     1741 aatcacgacg gtgatttgga tcgtgccaag ctactcatcc gcctcgccaa agaagccgga
     1801 gccgatgctg ccaagttcca gcattttcaa gcgccgaaga tcgtttcaga gtacggtttc
     1861 actcatatga attccaaggt cagccaccag gcaaattgga agcgttccgt taccgaagtc
     1921 tatgcggcgg cttctgtttc tcaaagttgg acgcccattc tcaaagaaga atgcgataaa
     1981 gttggtatcg acttcttcac atcaccgtac gattatgact cgattgagca tgtcaatcca
     2041 tacattccag cttttaaagc gggctctggc gaaattgact ggatcgaagc gctcgtccac
     2101 atggcgtcca aaggcaagcc tatgatcatt gcctgcggcg ccgctgatat cgctgacgta
     2161 cagcgtgccg tccaagccat tctcaaagtc aacaaacagt tgtgtctcat gcaatgcaac
     2221 acaaactaca ccgccaaccc caataactac gatcacctgc atttgaacac gctcaaaacc
     2281 ttcgccgcca tgtaccccga tgtgattctt ggtttgtctg accatacgca ttctgttgcg
     2341 ccggttctcg gcgcgatcac tctcggcgca cgcatgattg aacgtcactt cactgacagc
     2401 aacgaccgcg aaggacctga tcacaagttt gcgatgaatc ctgccaattg ggcgaagatg
     2461 gtcgaagaga ctcgtttgct cgaacgttcg ctgggtagct ccgataaatt cattgctgcc
     2521 aatgagcaag agactaaagt cgttcaacgt cgctgtctgc gagcagcccg cgatatcaaa
     2581 gccggtgaga ccttcacccg cgatatgatc gatgttctgc gccccgccac accgggcgcg
     2641 gtcaaaccgc atgagatcga gaatgtcatc ggcacgaagg cgttagtcga tatgccaaaa
     2701 ggcaaggaat tgcgctgggt ggatttaggc gcttaggaag tcccatgtgt caggttccac
     2761 gttccaagtt gggaggttga tgtgacggct gtgactaagt ttgaagattt aatcgcatgg
     2821 caggaagcac gtacgttagt gaaatgggtt tacaaaatta cttcggatgg tgcattctcg
     2881 aaggattatg gcatgcgaga tcaaatccaa cgtgcggctg tttctgtaat gacgaacatc
     2941 gctgagggct tcgattgtga gtcttctgct gaatttgccc gttttcttgg tatagcacgc
     3001 cgctccgcag ttgaggtgca gtctctactt tatgcagctt tggatgtgga ccatatcaaa
     3061 gaggatgttt ttaaattaca ttacgaacag gcgaaaaagt gcaaagcgtt gattggtggc
     3121 ttgaaacaaa gcgtcctgaa aaatccacgt cgcaacaaca ctccatctcg cgcctgatac
     3181 ctggaacttg acacttggaa catgaaactc atttatttct ccctcggcta ctccacccac
     3241 gattatcgtt ttctcaaagc catttcggat ggcgggcatg atgtccactt tgtgcagctt
     3301 gaaggcaacc ggcggcaggt tgaaagtcgg tctgtgcctg agaatgtgaa tcaagtcatt
     3361 tggaagggcg gacgcgaagc ttttacgtgg aacaaactcc cagccttggt gatggatttc
     3421 aaacgcctct tacgggacct aaagcctgac ctagtccatg cgggtccgat ccaaacctgt
     3481 gcttttatcg ccgtcctcgc tggcgcgaga ccactcctca ccatgtcttg gggattcgat
     3541 ttgatggacg acgttcacaa agggtggatg tgggaattcg ccacgaagta cacactcaaa
     3601 cgttctacat tctttaccag tgatgcgaat gtgactaaag ataaagccgt tgcgtatggc
     3661 atgaatccag aaaagaccat cgtcttccct tggggtgtgg acttggatca tttcacaaag
     3721 aaagaagaaa tagatagtaa agaaggtttc gttctatttt gcaaccgctc ctgggagaca
     3781 cgttacgggg tggatgtgct ggcgcgcgcg ttcgttaaag tggcaagcca gagggaggat
     3841 atacggttat tgcttttagg cggcgggtcg cagggagcct atcttcgcaa gatttttcaa
     3901 agcggcggtg tggacgaata cgtcacctat ggcgggcaga tctcgcagac ggatcttccg
     3961 cgctggtatc atatggcaga cctgtacatt tcaccttcgc atgtggacgg ttcatctgtg
     4021 tcgttgatgg aagccttggc gtgtgggttg ccttgtctcg tgtcggatat ccctgccaat
     4081 aaagaatggg tattcgagaa tcagaacggc tggctgttcc gcgatgggga tgtggatcac
     4141 cttgcagaga agattctggc ggcaatgaat cagcgtgaga aactgcctga gatcggcaga
     4201 gcaagccgaa gtgaagcaga gaagcgtgcg gattggaaaa agaatgccgc ggcgttgatg
     4261 gaagtctatc gaagtctaaa atgatacgtc attgcgaggg cgatgttctt tcgcccgaag
     4321 caatctcata attgagtaca agattgcttc gtcaagctgt cgccctcctc gcaatgacgg
     4381 gaaaaggaaa atatgttaag taaaaaagaa ttactggatg gttttggtca actcaacttg
     4441 aaaaaag
//
LOCUS       DAOQFT010000151         4433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10506_length_4433_cov_7.217451, whole genome shotgun sequence.
ACCESSION   DAOQFT010000151 DAOQFT010000000
VERSION     DAOQFT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10506_length_4433_cov_7.217451"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="PLR93_05170"
     CDS             <1..1315
                     /locus_tag="PLR93_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNH26387.1"
                     /translation="NAFSFWIAVPGAVLLLMSLFLGGFDTGWTGYPPLSARAPVGMQM
                     FFLGVFVAGWSSILGSLNVIVTVLRMRAKGMTAWRMPILIWASLATALIALTATQLIG
                     LSFQLVMFQRLFGMGFFDPAKGGNPVLFQHLFWFYSHPAVYVFILPGLGVISELLPVF
                     VRKPLFGYRWIAMSSLGIALVGFVVWAHHMYTSGMNEYLRVPFMYSTLLVAVPTGVKF
                     FSWVATIWKGKISTPTPMLFVLGGIIVFLLGGLSGPPNATVSTDLQLQDTYWIVGHFH
                     DTIFGGFVFPFFAAIYFWFPKATGRKMNETLGKIHFWLMTPSFFALTLGMMYAGLLGM
                     RRRIVDYDPALGIDSIHLVMTIGAFLIALSVLIFFFNFFNSIKNGEKAEGNVWNSRSP
                     EWQVPSPMPAHNYDKPFEVIGEPYDYGLNDGGFVKFIEPAKNSKH"
     gene            1335..2126
                     /locus_tag="PLR93_05175"
     CDS             1335..2126
                     /locus_tag="PLR93_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNH26388.1"
                     /translation="MAHSQDKSSALGQGVVIFVYLAVLTLIEYFIAVTFNATALLLVV
                     AVIKAALVVYYYMHIYELNAESGEHEDENSYEYKTGTNRIGLWLFLLSDAFVFAGLLV
                     MRSNLLGLTRPHLNQFLGLGVTAVLLISSFFMNRGETAMKHGDVKAFMNNTIITFVLG
                     LGFLLGVVLVEWRLAAEEGLVASFGNPSVGPLGGVFYMMTGMHAFHVFTGLIFLFVVW
                     NNARKGVYSAEKHWGVEAAAVYWHFVDLVWIFFYPALYLIGTAVK"
     gene            2189..2800
                     /locus_tag="PLR93_05180"
     CDS             2189..2800
                     /locus_tag="PLR93_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH26389.1"
                     /translation="MDKKLLWTGGGILAIVVIAGLLSFIFANPPSFRGTLYGEPFPSA
                     AEIELTKADGSIFRLSDQRGKVVLLFFGYTSCPDVCPTTLAEMKLVMDKLESDAEQVQ
                     VVFVSVDPDRDTPEKIQTYVEHFNPTFLGLSGSLSELEPIWNSYSITREAVESDSALG
                     VIINHTARLFLVDPQGQMHLSFAYQTPVDDIVHDIRLILDQEK"
     gene            2809..3294
                     /locus_tag="PLR93_05185"
     CDS             2809..3294
                     /locus_tag="PLR93_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26390.1"
                     /translation="MKRILWSFLLGLVLAAITTELAYFLLKKENREPGVIELVIPAGT
                     AEKVAAGQAPLEIPKDMRFVVGDTLKVINQDTENHQLGPLWIPANSSATQKLETAENL
                     VVECSFQAGSVIGITVQEPVTWRTRISGIFFAGFPLGMMFAVYSVVVVPKKKEDNEHP
                     S"
     gene            3278..4015
                     /locus_tag="PLR93_05190"
     CDS             3278..4015
                     /locus_tag="PLR93_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNH26391.1"
                     /translation="MNILRKMFSRKWVLTTLLAFAGTAVCIRLGIWQLDRLEQRRAFN
                     HQVESMRAAELLDLNSEVPEDIASMEWRAVTITGNYDFENQVALRNQYNAGLYGFHII
                     TPLLFDGGAVLVDRGWIPAESDSPPAEWRMYDEDEQVTVTGQIRLGHGKPAFGGVVDA
                     LPVDGSKLNVWNNLDVEKISTQMPYPILNVYIQPNANVSDTVPPIPFQPTIELTEGSH
                     FGYALQWFTFATILFVGYPFFLQKQDD"
     gene            4027..>4433
                     /locus_tag="PLR93_05195"
     CDS             4027..>4433
                     /locus_tag="PLR93_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26392.1"
                     /translation="MKTSFASYTALLVVVVFALTVVGRLVTITGAAFVCEGWPLCLPT
                     APIGYLKLMHLGLVGLASIVMFLVWRKAWREQRHHAILLPLTTITGVMFIGQAFVGAI
                     EVTRQYPTHIVVLHTLTAIGLWISLAMLVFVSGT"
BASE COUNT          966 a         1016 c         1090 g         1361 t
ORIGIN      
        1 caatgccttc tcattctgga ttgctgtccc tggggctgtg cttctgctca tgagcctttt
       61 cctcggcggt tttgatactg gttggacggg ttacccgcct ctgtctgccc gcgctcctgt
      121 cggtatgcag atgttcttcc ttggcgtgtt cgttgccggt tggtcatcca ttcttggttc
      181 tttgaacgtt attgttactg tgcttcgtat gcgtgccaaa ggcatgaccg cttggcgcat
      241 gccgatcctc atttgggcat ctcttgccac tgcactgatc gcactgaccg ctacacagtt
      301 gatcggtctt tctttccagt tggtgatgtt ccaacgtctc tttggaatgg gcttctttga
      361 ccctgccaaa ggcggcaacc cggttctctt ccaacacctg ttctggttct actctcaccc
      421 tgcggtgtac gtattcattt tgcctggctt gggtgtgatc tctgaattac tgcctgtgtt
      481 tgtccgcaag cccctcttcg gttatcgctg gattgcgatg tcctctttgg gcattgcttt
      541 ggtgggcttc gttgtgtggg cacaccacat gtatacctct ggtatgaatg aatatttacg
      601 cgtacctttc atgtacagca ccttgttggt ggctgtaccg acgggcgtta agttcttctc
      661 ctgggttgcg acaatttgga aaggcaagat ttcaactcct accccaatgc tctttgtgtt
      721 gggcggtatt atcgtcttcc ttttaggtgg tctctcgggt ccccccaatg cgacagtctc
      781 taccgacctt cagttacaag acacctattg gatcgtaggg cacttccacg acaccatttt
      841 cggtgggttt gtcttcccat tctttgctgc catctacttc tggttcccca aagcaaccgg
      901 acgcaagatg aacgaaaccc ttggcaagat tcatttctgg ctgatgaccc cttcgttctt
      961 tgccttaacc ttgggcatga tgtatgctgg cttgctcggt atgcgccgcc gcatcgtgga
     1021 ctatgaccct gctttgggta ttgactcaat acatttagtt atgaccattg gtgcgttcct
     1081 gatcgccctt tccgttttga ttttcttctt caacttcttc aatagcatca agaatggaga
     1141 gaaggcagaa ggcaacgttt ggaattcgcg ctcccctgaa tggcaggttc cttcccccat
     1201 gcctgcccat aactacgata agccattcga agtgatcggc gaaccgtatg actacggttt
     1261 gaatgatggt ggctttgtta agttcattga acctgccaag aatagcaagc actaaaggcg
     1321 aggagagtaa ttaaatggca cattcacaag ataaatcgtc cgctcttggt cagggcgtgg
     1381 tgatctttgt atatttggca gttctgaccc ttattgaata cttcattgcc gtaacattca
     1441 acgcaacagc ccttttgctg gttgtggcag tgatcaaagc cgccttggtt gtgtattact
     1501 acatgcacat ctatgagttg aatgcagaaa gcggcgagca tgaagatgaa aactcttatg
     1561 agtataagac cggcacaaat cgtataggtc tttggctgtt cctcttgtca gacgcatttg
     1621 tgtttgctgg tctgttggtg atgcgttcta acttgcttgg gcttacccgc ccgcacctca
     1681 accagttctt gggtttgggt gtaacggctg tgttgctcat atcttccttc ttcatgaacc
     1741 gtggcgagac tgccatgaaa catggcgatg tgaaagcctt tatgaacaac accatcatca
     1801 cctttgttct gggcttgggc tttttgcttg gcgtggtgct cgtggaatgg cgcttggcgg
     1861 ctgaagaagg tttggtggct tcatttggca acccgtctgt cggtccgttg ggcggcgttt
     1921 tctacatgat gaccggtatg catgccttcc atgttttcac tggtttgatc ttcctctttg
     1981 ttgtgtggaa taacgcccgc aaaggtgtgt actctgctga aaaacattgg ggtgtagaag
     2041 ccgctgcagt ctattggcac ttcgtagatt tagtttggat tttcttctac cctgccttgt
     2101 atctgatcgg cacggctgta aaataaaatg aatacctccg ccgccgatga ggcggcggtt
     2161 ttttttcgac attaatcgga gataaaatat ggataaaaaa ttactgtgga cgggcggagg
     2221 catccttgct attgttgtaa tagcagggtt gctttctttt atctttgcga acccgccctc
     2281 ctttcgcggc acactatatg gtgagccttt cccgtctgca gctgagattg agttgacaaa
     2341 agcagatgga agcatatttc gcctgagcga ccagcgcgga aaagttgtgc tgctgttttt
     2401 cggctacact tcttgcccag atgtctgccc aaccactctt gccgaaatga aattggtgat
     2461 ggataaactc gaaagtgacg cggaacaagt acaggttgtt tttgtgtccg tggacccaga
     2521 ccgcgatact ccagaaaaga ttcagaccta tgtagaacat ttcaatccca ccttcttggg
     2581 tctatcggga tctttatctg aacttgagcc tatctggaat agttattcca ttacacgcga
     2641 agccgtagaa agtgactcgg cactgggtgt aattattaac cataccgcac gtttattcct
     2701 tgtcgaccca caagggcaga tgcatctttc ctttgcatac caaacccctg tagatgatat
     2761 tgttcacgat atccgtttga ttttggatca agaaaaatga atttcacaat gaagcgaatt
     2821 ttatggtcat ttcttttggg gcttgtattg gcagctatta caacagagct ggcatacttt
     2881 ctcttgaaaa aagaaaaccg cgagcccggt gttattgaat tggttattcc tgcgggaact
     2941 gctgaaaaag tcgctgctgg tcaggctccg cttgaaatac cgaaagatat gcgatttgtc
     3001 gtaggcgata cattgaaagt gataaaccaa gacactgaaa atcatcaact gggtccgtta
     3061 tggattcctg ctaattcttc tgctacacaa aaattggaaa cagcagaaaa tttagtagtg
     3121 gaatgttctt tccaagccgg gagtgtgatc ggtattactg ttcaagaacc tgttacatgg
     3181 cgcacaagga tttcaggcat tttctttgct ggctttccgc ttggaatgat gtttgctgtt
     3241 tacagtgtag tggttgttcc caagaagaaa gaagacaatg aacatccttc gtaaaatgtt
     3301 ttcaagaaaa tgggtgctta ctacgttgct ggcttttgcc ggaacggcgg tttgcattcg
     3361 tttgggaatt tggcaactag atcgccttga acaacgccgc gcatttaatc atcaagtgga
     3421 aagtatgcgc gcggcagaat tattggacct caatagcgaa gtccctgaag atattgcttc
     3481 aatggaatgg cgggcagtca ccatcaccgg caactatgac tttgaaaatc aggttgcttt
     3541 gcgaaaccaa tataatgcag gcttgtatgg attccacatc atcaccccgc ttcttttcga
     3601 cgggggagct gttctggtag accgcggctg gattcccgct gagagcgact cgccacctgc
     3661 tgaatggcgt atgtacgacg aagatgaaca agtcacggta acagggcaga tacggcttgg
     3721 gcatggcaaa ccagctttcg gcggagttgt cgatgcgttg cctgttgatg gctccaaact
     3781 caatgtgtgg aataatctcg acgtggagaa aatatccaca cagatgcctt atccaattct
     3841 taatgtttat attcaaccca atgccaatgt cagtgacacg gttccgccaa tcccatttca
     3901 gcccacaatt gaattgacgg aaggctcaca tttcgggtat gctttgcagt ggtttacctt
     3961 tgcgacgatt ttgttcgtag gttacccatt ttttctgcaa aaacaggacg attgatgaaa
     4021 tattctttga aaacttcttt tgctagctac acagccttgt tggttgtcgt agtttttgcg
     4081 ctcactgtgg tggggcgttt ggtgaccatt actggggctg cctttgtgtg tgaaggttgg
     4141 cctttatgtt tgcctactgc tccgattggc tatttaaagt tgatgcattt gggcttggtt
     4201 gggttggcat ccattgtgat gttcctcgtg tggcgaaagg cttggcgtga acagcggcat
     4261 catgccatct tgttgccatt gacgacgatc acaggcgtta tgtttattgg gcaggcattt
     4321 gtaggggcaa ttgaggtgac gcgccagtac ccaacccata tcgtggttct tcatacacta
     4381 acagcaatcg ggctttggat ttcgttggca atgcttgttt ttgtgtctgg cac
//
LOCUS       DAOQFT010000152         4424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10541_length_4424_cov_6.780041, whole genome shotgun sequence.
ACCESSION   DAOQFT010000152 DAOQFT010000000
VERSION     DAOQFT010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10541_length_4424_cov_6.780041"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLR93_05200"
     CDS             complement(<1..205)
                     /locus_tag="PLR93_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNH26393.1"
                     /translation="MQDKLNEIEKSALAALAVVNDPAAVEAWRVTHLGRSSELMGVFS
                     GLGKLSKEERPAVGQAANRVKVAL"
     misc_binding    complement(346..591)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            647..1291
                     /locus_tag="PLR93_05205"
     CDS             647..1291
                     /locus_tag="PLR93_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HNH26394.1"
                     /translation="MTASPLFEFALLSFISMFTMVNPIGVVPVFTAMTAKLTPQESHR
                     VARKATFTGAVILFAFALTGQWIFRFFSISVDSLRVVGGVIFFFMGYEMLQARLTRTQ
                     FDDETNHEYINDISITPLGIPIICGPGAITNAILLMNEADTMVEQGIVLGVIAIILLF
                     TYLLLLGAKQVTRIIGENGNKVMLRLMGLIVMVIAVEFFFAGLTPIVRKMLSGG"
     gene            complement(1375..2862)
                     /locus_tag="PLR93_05210"
     CDS             complement(1375..2862)
                     /locus_tag="PLR93_05210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNH26395.1"
                     /translation="MKNKYILLSAIILLALSCKALTPTTPTALPSTEAVPTEDLPATE
                     PAVDEPIDPLAGSPGLGDSLYPDFGNGGYDVTSYLLDITVHDVKTSDLTAITTIEAVA
                     TQDLSQFNLDFAGFEITSITVNDEAAEFERDGQELTVIPAQALANGEAFTTVLEYQGV
                     PASMTSKALPFPTGWIAYENGIFVLSEPDGSASFYPVNDHPLDKAKYTIMVTVPKPYE
                     VGANGVLEETIDNGETTTYTFTVRDEMASYLTTININEFNTETMTSDGGVPIRNYYAT
                     SLPDGVNKPFARQGEMIDYFSELFGPYPFEVYGALVMDTEFGAALENQTMSIFGIDMV
                     DLEDIEGTEAVVAHELVHQWFGDSVSVGDWGSIWLNEGFASYGEGLWEEHLNGRDALD
                     EWVNGVYEEVKAYPEFYPPPGNPPANDLFNGGVYLRGGLTLHALRLEVGDDVFFEILR
                     TYYDRYKYGNAVTDDFIAVAEEVSGKDLGDFFNAWLYEEQLPELP"
     gene            complement(2852..3532)
                     /locus_tag="PLR93_05215"
     CDS             complement(2852..3532)
                     /locus_tag="PLR93_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH26396.1"
                     /translation="MTNEEIIQKTAEYIKQEFSDDSSGHDWWHIYRVWKNAIAICEIE
                     EADPFIVQLAALLHDLDDWKFNEAGDETPHRAKAWLDSCSVSPNIADTVCKIIMHISY
                     KGAGVENKMDSLEGFIVQDADRLDAIGAIGIGRAFAYGGYKNRMMYDPDSPPAMHASF
                     EEYKNSKSATINHFYEKLLLLKDRMNTSTAKRIAEERHDVMVKFLDQFMLEWEGADIT
                     NSSGTYEK"
     gene            complement(3534..3956)
                     /locus_tag="PLR93_05220"
     CDS             complement(3534..3956)
                     /locus_tag="PLR93_05220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HNH26397.1"
                     /translation="MIDFKSKLGRKALRHLKQEYFIWLTTVDSKGTPQPRPVWFVLEG
                     DSLVVYSQPSAFKLKHILNNPNVSLHFNTPDPKGEEDVIVFSGVAKIDSKGEPVNKNG
                     PYLRKYRSGIKGLNSTPQQFTQTYSTTIRIKLTSLRGW"
     gene            complement(4069..>4424)
                     /locus_tag="PLR93_05225"
     CDS             complement(4069..>4424)
                     /locus_tag="PLR93_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26398.1"
                     /translation="WVIAMLVAVTAGYMLFDGVHALRTGDYITPSEGEYAGQLGPWAG
                     LVASVGIDPHSTLMKTIFVAYGFSALVGVAGFLTDQVWGRSALLIMAVLGLWYLPIGT
                     AVNIVVLMLLFLKRN"
BASE COUNT         1090 a         1102 c         1075 g         1157 t
ORIGIN      
        1 caagcgccac cttcacacgg ttcgcagcct gaccgacagc gggtcgctcc tctttggaga
       61 gtttgcccaa gccggaaaag actcccatca attccgagct gcgcccgagg tgggtcacac
      121 gccatgcttc cactgctgcc gggtcattga ccactgccag cgctgccagc gcagattttt
      181 cgatctcgtt caatttgtcc tgcatacatg cctccattat tcccgtcatt gcgagggcgg
      241 tgcttttccg cccgaagcaa tctcaaatat gttgtgcgag attgcttcgt cgggctgtcg
      301 ccctcctcgc aatgacggat agatacaaac aaaaactccc gtccacaatg ggacgggagg
      361 aagttcaccc gtggtaccac ccaaattcat ctcaattgag atgcactttg tgccgactaa
      421 catcggcttc ccttataacg ctgggattgc gattcagact actagaaaat cattcacctg
      481 aacggctcga gagggaactt caactgggtt cggacgagtg caatttcacc aactgccttg
      541 cacatctctg gcggcttccg tcagcctact ttcctctgtc actgcctttg tgcgggtaat
      601 tatctgcgga atcaggctcc tgtcaagtgc gggtaaaatc aacgccatga ccgcatctcc
      661 tctttttgaa tttgccctgc tcagctttat ttcgatgttc accatggtca acccgattgg
      721 ggttgtacct gtcttcactg ccatgaccgc caagctaact ccgcaagagt cgcatcgggt
      781 ggcacgcaaa gcgaccttta ccggtgccgt gattctcttc gccttcgcgt tgaccggtca
      841 gtggatcttt cgcttcttct caatctctgt ggacagttta cgggtagtgg gtggtgtgat
      901 ctttttcttt atgggttatg aaatgttgca agcccgcctc acgcggactc aatttgatga
      961 tgaaaccaat catgaataca tcaacgatat ttccattacg ccgttgggca ttcccatcat
     1021 ctgtggaccg ggcgccatta ccaatgctat ccttttgatg aacgaagccg atacgatggt
     1081 agaacaaggc attgtgttgg gagtgatcgc tattattttg ctttttacat atttattatt
     1141 acttggcgca aaacaagtca cacgcattat tggggaaaat gggaataagg tcatgttgcg
     1201 tttgatgggg ttaatcgtaa tggtgatcgc cgtggaattt ttcttcgccg ggttgacacc
     1261 aatcgtacga aaaatgcttt cgggtggcta aaggctcgga gcgctaaagc aggtttgagt
     1321 taagttggaa gcgtaaaaga gacagtccca ttacatggga ctgtctcttt tatcctatgg
     1381 aagttcaggg agttgttctt cgtacaacca ggcattgaag aaatcgccga ggtctttgcc
     1441 gctgacttct tcagcaacag cgatgaaatc atcggtgacg gcgttgccat atttataacg
     1501 gtcataatat gtgcgcagaa tctcaaagaa cacatcatcg ccgacctcaa gccgtaaggc
     1561 gtggagcgtg agcccgccgc gcaagtaaac gccgccattg aagagatcgt tggctggggg
     1621 gttgccaggc ggagggtaaa actctgggta tgccttcact tcttcataca caccattaac
     1681 ccattcatca agtgcgtcgc gcccgttgag gtgttcttcc caaagacctt cgccatatga
     1741 agcaaaacct tcgttgagcc aaatactgcc ccaatcgcca acactgacgc tatcaccaaa
     1801 ccattgatgg acaagttcat gagcaaccac tgcttctgtg ccttcaatat cttccagatc
     1861 caccatgtcg attccaaaaa tggacatggt ttggttttca agcgcggcac caaactctgt
     1921 gtccatcacc aaagcaccat acacttcaaa tggatacgga ccgaaaagct cgctaaagta
     1981 gtcgatcatc tcgccctgac gcgcaaaagg tttattgaca ccatcgggca aactggtggc
     2041 gtaataattg cggatcggaa caccaccatc agatgtcatt gtttctgtat tgaattcatt
     2101 gatgttgatg gtagtgagat agcttgccat ttcatcacgg acagtgaagg tataggtggt
     2161 cgtctcgcca ttatcgatgg tttcttcaag aacgccgttc gccccaactt catagggttt
     2221 gggcacagtg accataatgg tgtacttcgc cttatcgagc ggatggtcat tgaccggata
     2281 aaaactggcg gaaccatcgg gttcgctcaa gacaaagatg ccgttctcat atgcgatcca
     2341 accggtgggg aaaggcaacg ctttagacgt catcgatgcg ggaacacctt gatactccag
     2401 cacggtagtg aaagcttcgc cgttggctag cgcttgcgct gggatgaccg ttaattcctg
     2461 cccgtcacgt tcaaactctg ccgcctcatc gttgaccgtg atgctggtaa tttcgaaccc
     2521 ggcaaaatcc aggttgaact ggctcaaatc ttgagttgca acagcttcga tggtagtaat
     2581 tgccgttaga tcgctggtct tcacatcgtg gacggtaata tccaaaagat aggatgtgac
     2641 gtcataaccg ccgtttccaa aatcggggta aagagaatct cctaaacccg gagaacctgc
     2701 caaggggtct atgggttcat caacagcggg ttctgttgcg ggtaaatctt ctgtaggcac
     2761 agcctctgtg gaaggaagcg ccgttggcgt agtcggagtc aatgctttgc aagataatgc
     2821 cagcaaaatg attgcagaaa gcagaatata tttatttttc ataggttcct gatgaattcg
     2881 taatatctgc accttcccat tcgagcatga actggtcgag aaatttcacc atcacatcgt
     2941 gccgttcttc ggcaatgcgc ttggcagtgg acgtgttcat tcggtctttg agcaggagca
     3001 gtttttcata aaagtgattg atcgtagcgc ttttgctgtt cttgtattct tcaaagctag
     3061 catgcatggc aggtggagaa tctgggtcgt acatcatgcg attcttatag ccgccatagg
     3121 cgaaggcacg tccgatgccg atggcgccaa ttgcatcgag acggtctgca tcttggacga
     3181 taaagccttc gagcgagtcc attttgtttt ctacaccggc acctttgtag gaaatgtgca
     3241 taatgatctt gcacacggtg tcagcgatgt ttgggctcac agagcaagag tcaagccagg
     3301 ctttggcgcg atgcggcgtt tcgtcgcccg cttcgttgaa cttccaatca tcgaggtcgt
     3361 gtaagagagc agcaagctga acaatgaatg gatcagcttc ttcgatctca cagatggcga
     3421 tggcattctt ccacacacga taaatgtgcc accaatcatg cccagaagag tcatctgaaa
     3481 actcttgctt gatgtactcc gcggtctttt gaataatctc ttcgttcgtc atatcaccaa
     3541 ccacgcaacg atgtgagttt gatgcgaatc gttgtggagt atgtttgcgt aaattgctgc
     3601 ggcgtggagt ttaatccctt aatgccgctg cgatacttgc gcaggtaagg tccgttcttg
     3661 ttaactggct cacctttgga gtcgatcttt gcaaccccgc tgaacacaat cacatcttct
     3721 tcgcctttag ggtctggggt gttgaaatgc aaagagacat tggggttatt cagaatgtgc
     3781 ttgagcttga aggcactggg ctggctgtaa acaacaagag aatcgccttc caacacaaac
     3841 caaaccggtc gcggctgagg tgtgcctttc gagtcgacgg tggtgagcca gataaagtat
     3901 tcctgcttga ggtgacgtaa ggctttgcgc cccagtttgg atttgaaatc gatcatttct
     3961 tcctccattc ataaattcga taatgcctgc acttaaaatc attataaacg aacaggctgg
     4021 gcatgttgcc cagcctgctc ttacggatga aatatttttt catgatattc aatttcgttt
     4081 caggaacaag agcatcaaca ctacaatgtt gaccgcagta ccaataggga gataccacaa
     4141 cccgagcact gccatgataa ggagagcact acgcccccaa acctggtctg tgagaaaacc
     4201 agcaacccca actagtgcag aaaaaccata agcaacaaaa atggttttca tcaatgtgga
     4261 gtgtgggtca atgcctacag aagcgacgag cccagcccaa ggtccgagtt gacccgcgta
     4321 ttcgccttca gatggcgtaa tgtaatcgcc cgtccgcaag gcatggactc cgtcgaagag
     4381 catgtatcct gccgtgacgg caaccagcat ggcaatcacc cact
//
LOCUS       DAOQFT010000153         4419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10558_length_4419_cov_7.212878, whole genome shotgun sequence.
ACCESSION   DAOQFT010000153 DAOQFT010000000
VERSION     DAOQFT010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10558_length_4419_cov_7.212878"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..686
                     /locus_tag="PLR93_05230"
     CDS             135..686
                     /locus_tag="PLR93_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26399.1"
                     /translation="MDSKPTFDNFPLLSTPRLLLRDLRPTDLDDLYEYASDPEIDRYT
                     PWDRYQSLDEAREDLGGYIARYEQGHFRAWGIEHRADKKLIGITNFGFLSKEDRRAEM
                     GYTIARKYWGQGFATEAAQALIRYGFEKLDLARIEAVVLPENKASSKVMLKVGMQYEG
                     LLRSYQVWKGKPSDLEIYAIVKA"
     gene            688..1209
                     /locus_tag="PLR93_05235"
     CDS             688..1209
                     /locus_tag="PLR93_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26400.1"
                     /translation="MAIILETERLILRHQVLSDLDDLWALYQDPEITKYIPDAPRSRE
                     EAQKELEWHMHGHPRRPELGLWATIHKETGKFIGRCGLLPWEIDGQNEVEVAYTIAEA
                     YQGQGLGSEAALAILNYGFGKLRLSRLICLIDPENIPSQKVAEKMGMRFEKESRDEYG
                     MFWIYSIKKEAGN"
     gene            complement(1233..3209)
                     /locus_tag="PLR93_05240"
     CDS             complement(1233..3209)
                     /locus_tag="PLR93_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH26401.1"
                     /translation="MNILVTLCLGGLGVLFLVLLTGIRFIPNNRIGIVEKRFGGKSLK
                     SGLIAINGETGFQPQVLRGGLHYRMPIQYAIHIAPLVTITQGKIGYVFARDGAPLEPA
                     QVLASNVTAHDFQDVTTFLKNGGQKGPQRLILREGTYAFNLMQFVVITEERVYSLPLS
                     RDDTAVIVRMAEVIKERDGFRPVIIKDGDDKIGIVTVHDGPSLPQGEIIAPVVGDNPE
                     KADEYHNKFQDADRFLKANGMRGRQLQVLVEGTYYINRLFATVEMIHKTIIDVGNVGV
                     VVSYTGEQGVDLSGSEYRHGELVTRGQRGVWSEALLPGKYPFNTYAGKVIPVPTTNFI
                     LKWIKNEVGSHNFDENLTEVSLITKDAFEPSLPLSVVVHIDYKKAPLVIQRFGDIKRL
                     VEQTLDPMVAAYFKNVAQTRTLIQLLQERSAIQQQSSLEMKEKFEHYNLELEEVLIGT
                     PSSPAGDKQIETILTQLRQRQIAEEQVETYSQQEKAAVKERELREAESRAAMQTKMTE
                     AELSISIQSNQGKAEYERSIQQAQQIRALAEAEAEKVARIGIAQALATEEQVRAYGGP
                     QFQVTQQVLNRFAEAIQQSGVDVVPRVVVGGSNGENGGNQSANIMEGLLTMLLSERFG
                     VSGEASTTQRSPDVEAMREKILKDMQKKDDTKKA"
     gene            complement(3263..3982)
                     /locus_tag="PLR93_05245"
     CDS             complement(3263..3982)
                     /locus_tag="PLR93_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26402.1"
                     /translation="MQNQSKSYNQFFIMFALGVLSGLVLITLATWADLEAAYYGFSRR
                     ASTPLSGFSCPVLMTATETNTVSLKLKNTTDGKISPTILVETSSPVAAFETTENLELA
                     AGEQTTAEWTVGPENLDLKRFIFAKALVYSSYPFPDRETTCGIFVLNAPGNGTIIVWG
                     FVILSLLGMGIGLYGVHQFQGPVKRGADVFKQFLFLTIVVLTGFLSIYISSWLLGILV
                     LVVSILFVIISTGIVLGRHHI"
     gene            4163..>4419
                     /locus_tag="PLR93_05250"
     CDS             4163..>4419
                     /locus_tag="PLR93_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH26403.1"
                     /translation="MTDHIRNFCIIAHVDHGKSTLADRLLQLTGTVSERDMTDQALDS
                     MDLEREKGVTIKASAVRMFYTAKDGQKYELNLIDTPGHVDFG"
BASE COUNT         1091 a         1103 c         1100 g         1125 t
ORIGIN      
        1 gaaaagtctc cgattcggat catacgtagg cgccttcatt ctcttataca ccctccagta
       61 acgggagagt caagtgctgg gcattaggac ttatatccca gcatgttttt tggtagatat
      121 tctataatca cagcatggat tctaaaccta cattcgataa cttcccgctt ctttctaccc
      181 cgcgcctgct cttgcgtgac ctacgcccga ccgacctcga cgatttgtac gagtacgcct
      241 ctgaccctga aattgaccgt tacaccccat gggaccgtta tcagtctttg gatgaggcgc
      301 gtgaagactt gggtggttat attgctcgct acgaacaggg acatttccgc gcctggggca
      361 tcgaacaccg cgccgataaa aagttgatcg gcatcaccaa cttcggcttt ctctcaaaag
      421 aagaccgccg cgccgagatg gggtacacca ttgcgcgcaa atattggggg cagggcttcg
      481 ctactgaagc ggctcaagcg ttgataaggt atgggttcga gaaactggac ttagcgcgga
      541 tcgaggcggt agtccttcca gaaaataaag cgtcttccaa agtcatgctc aaagttggga
      601 tgcagtatga aggtctactg cgcagttatc aagtatggaa aggcaaaccc agcgatttgg
      661 aaatctatgc gattgtgaag gcataagatg gctattattc ttgaaactga gcgtcttatt
      721 cttcggcatc aagttctttc agacttggat gatctttggg cgttgtatca agaccctgaa
      781 atcacaaaat acattcccga tgcgccgcgt tcgcgcgaag aagcacagaa agaactcgaa
      841 tggcatatgc acggacatcc gcgccgcccc gagttgggtt tgtgggcgac cattcacaaa
      901 gaaacgggca agtttatcgg tcgttgcgga cttttacctt gggagatcga cgggcagaac
      961 gaggtcgaag tggcatatac gatcgctgag gcgtatcaag gtcaaggctt ggggagtgag
     1021 gcggcgttag ccatcctgaa ctacgggttc ggtaaattga gattatcccg ccttatttgc
     1081 ttgatagacc cggagaatat cccttcgcaa aaagttgctg aaaaaatggg gatgcggttc
     1141 gaaaaagaaa gccgggatga atacggaatg ttttggatat attcaataaa aaaagaagct
     1201 ggcaactaat tgccagcttc tttttttata acttaggctt ttttcgtgtc gtctttcttt
     1261 tgcatgtctt tcaaaatctt ctcgcgcatg gcttccacat ccggtgagcg ttgggttgtg
     1321 ctggcttcac cagagacgcc aaagcgttcg gagagcaaca tggtcagcaa gccttccatg
     1381 atgttggcag attggtttcc gccattctcg ccattgctgc caccgaccac cacacgcgga
     1441 accacatcta cgccagattg ttgaatggct tcagcaaagc gattgagcac ttgctgtgtc
     1501 acctggaact gcggaccacc atacgcgcgc acctgttctt cggtggcaag cgcttgcgcg
     1561 ataccgatac gggcaacctt ttcggcttcg gcttctgcta aggcgcggat ctgttgcgcc
     1621 tgctggatgg agcgttcgta ctctgcctta ccctggttcg actggatgct aatgctcaac
     1681 tcggcttccg tcatcttggt ctgcatcgca gcacgggatt cggcttcacg tagttcgcgt
     1741 tccttcaccg cggctttttc ttgttgcgag taggtttcta cctgctcttc cgcgatctgg
     1801 cgctgacgta actgagtgag gatggtttca atctgtttgt cacctgccgg tgaggaaggt
     1861 gtaccgatta gcacttcttc caactcaagg ttgtagtgct caaacttttc cttcatctcc
     1921 aggctggatt gttgctgaat ggcactacgt tcctgcaaga gttgaatgag ggtacgcgtc
     1981 tgggcaacgt tcttgaagta tgcggccacc atggggtcga gggtttgttc aacgaggcgt
     2041 ttgatatcgc cgaagcgttg aatgaccagt ggggctttct tatagtcaat gtgaaccacg
     2101 actgaaagcg gcagggatgg ctcgaatgcg tccttggtga tgagcgagac ttcggtcagg
     2161 ttctcatcga agttgtgcga accgacttcg ttcttgatcc acttcaaaat gaagttagtg
     2221 gttggcacag ggatgacctt gcctgcatag gtattgaacg gatacttacc cggtagaagc
     2281 gcttcactcc acacgccacg ttgtccacgg gtgacgagtt caccgtgtcg gtattctgaa
     2341 ccggaaaggt caacgccttg ttcaccagtg taagaaacca ccacacctac attccctacg
     2401 tcaatgatgg tcttgtggat catctcaaca gtggcgaaca aacggttgat gtaatatgtg
     2461 ccttctacca acacttggag ttggcgacca cgcataccat ttgctttcaa gaagcggtca
     2521 gcatcttgga acttgttgtg gtattcatcg gctttctctg ggttatcacc taccactggg
     2581 gcaatgatct caccctgcgg taaagacgga ccgtcatgca cggtcacaat accgatcttg
     2641 tcgtcaccgt ccttgataat gaccgggcgg aacccatcac gttctttgat cacttctgcc
     2701 atgcgcacga tgacagccgt atcgtctcgg ctcaatggca gagagtacac gcgctcttca
     2761 gtaatgacca cgaactgcat caagttgaat gcatatgtac cttcacgcaa gatcaagcgt
     2821 tgcggacctt tttgcccgcc atttttcaag aatgtggtca catcctgaaa atcgtgcgct
     2881 gttacattgg aagccaagac ctgtgctggt tcaagcggag caccgtcgcg cgcgaaaacg
     2941 tacccgattt tgccttgggt aatggtcacc aagggtgcaa tgtggatcgc atactgaatg
     3001 ggcatgcggt aatgaagccc accacgcaac acctgtggct ggaaacccgt ctcgccatta
     3061 atggcgatca agccagattt caacgacttt ccaccgaagc gtttttccac gataccgata
     3121 cggttgtttg gaataaagcg gataccggtc agtaagacca aaaaaagaac gccaagccca
     3181 cctaaacata acgttacaag aatattcatt ttttctcctt caccatctaa atacgctgtg
     3241 aggcgtattc aggtttccaa ttttagatat ggtggcgtcc gagtacgata cctgtggaaa
     3301 taatcacaaa caggatggag acaaccagca caaggatgcc caacagccac gaactgatat
     3361 aaattgaaag gaacccggtt aatacgacaa tggtcaggaa taggaactgt ttgaacacat
     3421 ccgcgcctcg ttttactgga ccttggaact gatgcacccc ataaagcccg attcccatcc
     3481 caagcaggct caatataacg aagccccaaa cgattatagt cccgttaccg ggggcgttta
     3541 acacaaaaat accacaggtc gtttcacggt cagggaaagg ataggaagaa tagacaagtg
     3601 ccttcgcaaa aatgaagcgc ttcaagtcca aattttcggg tccaaccgtc cattcagcgg
     3661 tcgtttgttc gcctgctgca agctcaaggt tttctgtggt ttcaaacgct gccaccggcg
     3721 agctggtttc caccagaatc gtcggggaga ttttcccatc ggtggtgttc ttgagcttaa
     3781 gcgaaaccgt gttcgtttca gttgccgtca tgagaaccgg gcagctaaac ccgctcaatg
     3841 gtgtgctggc acgacgcgag aatccataat aggcggcttc caggtctgcc caggtggcaa
     3901 gtgtgatcaa gaccaacccg cttagaaccc caagtgcgaa cataataaag aattgattgt
     3961 aagattttga ttgattctgc atgtgatttc ctttaaaggc gatagtgatt ctataccctg
     4021 tctcttgttt tgccatggtg atttagtagg gttttcgccg cgttgccctc aaagattccg
     4081 tctacttatc gggtatactt cgttcgcttg ttgggtaaaa ataccgccaa ccaaaacatt
     4141 ttatagaaaa attgaaagta gtatgaccga ccatatccgt aatttttgta tcatcgccca
     4201 tgtagaccac ggcaaatcca cgctggcaga ccggctcctg caattgacag gtaccgtctc
     4261 tgagcgcgat atgaccgacc aagccctcga ctcgatggac ctcgaacgcg aaaaaggtgt
     4321 caccatcaag gcgtctgctg tgcgcatgtt ctacaccgcc aaagatgggc agaaatacga
     4381 actaaacctc atcgacactc ccgggcatgt ggacttcgg
//
LOCUS       DAOQFT010000154         4401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10622_length_4401_cov_5.115739, whole genome shotgun sequence.
ACCESSION   DAOQFT010000154 DAOQFT010000000
VERSION     DAOQFT010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10622_length_4401_cov_5.115739"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..714)
                     /locus_tag="PLR93_05255"
     CDS             complement(88..714)
                     /locus_tag="PLR93_05255"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNH26404.1"
                     /translation="MRLTNMITAVDLHACGEPGRVITGGVLDVPGKTMFEKMKYMEKN
                     LDDLRLRMLREPRGYPALCCNVIMPPTHPNAAAGFIIMEQTEYPPMSGTNTICVATTL
                     LETGMIPMQEPVTEFMLEAPAGLVGIRAECKDGKVTQVTFKNVPAFAVHLDLHVEVPT
                     LGTVKVDVAWGGMFYAIADASQFGLQLTPDEGKDITRITEMVKTATAE"
     gene            793..1218
                     /locus_tag="PLR93_05260"
     CDS             793..1218
                     /locus_tag="PLR93_05260"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26405.1"
                     /translation="MDYTRDQFTISNDTSRLDVDEICRFLSRSYWANQRERTVIEKSL
                     QGSLNFGVYDGDRQIGFARVVTDRATFAYLCDVFIHEDYRGKSIGKWMMECIMAHPDL
                     QGLRRWLLATYDAHGLYSQYGFTPLSDPTVMMEIVDRTK"
     gene            1304..3271
                     /locus_tag="PLR93_05265"
     CDS             1304..3271
                     /locus_tag="PLR93_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNH26406.1"
                     /translation="MLEELFSKPVGFFLIVMAIILITPLISERLRLPGIIGIIIGGML
                     IGPHGFGLLEDNDRIQFLSTIGLVYLMFSAGLEVDINQFMKVRTRALVFGIITFTFPQ
                     LMGMGLGYILGLNWLGMILLGSAFASHTLIAFPILTKLGVTRNEAVAVTTGATVLTDI
                     GAFIVLAVVLGADKGGLSFGYFAQLFVLLAVFTALIIFGLPRFGKFVFQKLTGRAVEF
                     QFVIVALFLAAFFAEVIGVHEVVGAFLAGLAVNSMLPRHSPVAGHVLFIGESFFIPVF
                     LLYSGLITDPLTFLKSPQTIIVAVGVTIVAYVSKLIAAWLTARIYKYTRSEFWTVYGL
                     SHAQAAVTIPTLVIGLETGLFDATLFNAAILMILLTSITSPLIVQRFAPDLQTASTDD
                     EQTPLFGRILVPVSEAHHSEGLVALSSLLARSSKGKVLAVSVAQDKGPNDNSSLMLHK
                     ELLGKVSANLHDPEAELELIPRLAATHAQGILHTANEENASLIVMGWRGKRTFRESVL
                     GSVLDEVIWGSDTPVVAGKLSLPLNSMQRVIFIVPAKAVPPIALRRMLEANLAIAKAL
                     NVPLLVRADKSYVQTLEALFVAISPEQEYKLEVLMDQLKPERLEHEAVSDFIILPGFG
                     SRKRVQDTLGNTPEQIAKYFDGNLALLHFDK"
     gene            complement(3337..>4401)
                     /locus_tag="PLR93_05270"
     CDS             complement(3337..>4401)
                     /locus_tag="PLR93_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH26407.1"
                     /translation="MKKTLVVFLAFLVAITACTEATPMPTHTPIPVTDTPTQTSTLEP
                     TATFTPAPTATPVPHPMSIIALRNQEYPGSEITIVKELDRGINYRRYYVYYLSEGLKI
                     YALLTIPNGEKPEEGFPAIVFNHGYIPPDVYKTTERYIAYVDQLARAGYVVFRIDYRG
                     HDQSEGEATGAYGDPGYQVDVMNALASLKQHPDVNPEKIGMWGHSMGGYLTLRAMVIS
                     NDVKAGVIWAGVVASYSDLLYNWRRTGAFTPSPSSTGRGWRSAWIEQYGTPEQNPAFW
                     DSISATSYLTDLSGPLELHHGTADEDVPVEFSIRLAELGRQSGQIVNLYTYEGDNHNI
                     SNYFTQAMNQTIAFFDIYLK"
BASE COUNT          964 a         1072 c         1161 g         1204 t
ORIGIN      
        1 atgtgcttgc gggtgcactg gcggtgcaga aagctgaccg atggtaggac ccgtaatttc
       61 tgggttatca ggatgtacaa ccggatatta ttcggctgtg gctgttttta ccatttcagt
      121 aatgcgggtg atatctttgc cttcgtcggg agtgagttgt aatccgaatt gcgatgcatc
      181 tgcgatggcg tagaacatgc caccccaagc gacatctact ttaacggtgc caagagtggg
      241 aacttccaca tggaggtcaa ggtgcacggc aaaggcgggg acgttcttga aggtgacctg
      301 tgtgactttg ccatctttgc actcggcgcg gatgccgacc agcccagcgg gcgcttcgag
      361 catgaactct gtgaccggct cttgcatagg gatcatgcct gtttcaagca aggtggtagc
      421 tacgcaaatg gtgttcgtgc cagacatggg cggatactcg gtctgctcca tgatgatgaa
      481 acccgcggcg gcattaggat gtgtgggcgg catgatgacg ttgcagcaca gggcggggta
      541 tccgcgtggt tcgcgtaaca tacgcaggcg caggtcgtcc agatttttct ccatgtattt
      601 cattttctcg aacattgtct tgccgggcac gtcaagcaca ccgccggtga tgacgcgtcc
      661 tggttcgccg caggcgtgca gatcaacggc ggtgatcatg ttggtgaggc gcatgggggt
      721 tctccatcaa gggtagaaaa tgaattcaga cgtagtgtac cgtaaatctg cccctgcctg
      781 ataaaataag ccatggatta cacacgcgac caattcacaa tttcaaacga cacatcccgg
      841 ttggatgtgg atgagatttg ccgattcctt tcccgttctt attgggcaaa ccaacgtgag
      901 cggactgtga tcgagaaatc ccttcaaggt tcgctcaatt tcggggtgta cgatggagac
      961 agacagatcg gttttgcgcg tgtggtgaca gaccgtgcca cgtttgccta tttgtgtgat
     1021 gtctttattc acgaagacta tcgtggcaaa tctatcggta aatggatgat ggaatgcatt
     1081 atggcacacc cagatctgca ggggcttcgc aggtggttgc tggcaacata cgacgcgcat
     1141 ggtttgtaca gccagtatgg tttcaccccg ctcagcgacc ccacggtcat gatggagatc
     1201 gttgatagaa ctaaatgagc cttgagagaa ttgggggatg gggaaattta ggctctccag
     1261 taaaattttt atttgtacgc caatccaact ttgagggaac tctgtgcttg aagaactttt
     1321 ctccaaacct gtaggctttt tcttgatcgt tatggcgatc atcctgatca caccgctgat
     1381 ctcggagcga ttacgtttgc caggcattat tggcatcatt attgggggca tgcttatcgg
     1441 tccgcatggt ttcggtctgc ttgaagataa cgaccgcatc caattccttt cgaccatcgg
     1501 cttggtctac ctgatgttca gtgccggtct tgaagtggat attaaccagt tcatgaaggt
     1561 tcgtacacgg gcattagtat ttggcataat caccttcact tttccgcaac ttatgggtat
     1621 gggcttgggc tacatccttg ggctcaattg gttgggcatg attcttctcg gttcggcatt
     1681 tgcttctcac accctcattg catttcccat tcttacgaaa ctaggtgtca ctcgtaatga
     1741 agcggtggcg gttaccaccg gggcgaccgt tctaaccgat attggcgctt ttatcgttct
     1801 ggctgttgtc cttggagccg ataaaggtgg tttgagcttt ggctattttg ctcaactctt
     1861 tgtgttgttg gcggtcttta cggcactcat tatttttggt ttgccccgct ttggaaaatt
     1921 cgtctttcaa aaacttaccg gtcgcgctgt tgaatttcaa ttcgtgatcg tcgccttatt
     1981 tttggcggca ttttttgccg aagtaatcgg cgttcacgaa gtggtaggtg ccttccttgc
     2041 aggcttggcg gtcaactcga tgttgccacg ccatagcccg gtggcagggc atgtcctctt
     2101 catcggcgag tcatttttca tccccgtctt tttgctttat agcggactca ttaccgaccc
     2161 gctgaccttc ctcaaaagcc cgcagaccat tattgttgct gtcggcgtca ctattgtggc
     2221 gtatgtttca aaactaattg ctgcctggct cactgcccga atttataaat acaccagatc
     2281 cgaattctgg accgtctatg gcttgtctca tgcccaagct gccgtaacca tccctacgtt
     2341 ggtcattggt ttagaaactg gacttttcga cgccaccctc ttcaatgccg ccattttgat
     2401 gattctcctc acctcgatca cttcgcctct tatcgtccag cgcttcgccc ctgaccttca
     2461 aactgcttct accgacgatg agcagacgcc actcttcggg cggattctcg tccctgtttc
     2521 tgaggcgcat cattccgaag ggttggtggc tctttccagc ttattagccc gcagttcaaa
     2581 gggcaaggta ttggcagtga gtgttgcgca agataaaggt ccaaatgaca atagcagcct
     2641 gatgttgcat aaggaattgc tcggaaaggt cagtgctaat ttacacgacc ccgaagcaga
     2701 acttgaatta atcccacgcc ttgcagcaac gcatgcacaa ggtattttgc acaccgccaa
     2761 cgaagagaat gcctccctaa tcgtcatggg gtggcgcggc aagcgcacct tccgtgagag
     2821 tgtgctcggt tctgttctgg atgaagttat ctggggttct gatacacccg tggtggctgg
     2881 gaagctatct ttgccgctaa atagtatgca gcgcgtaatc ttcatcgtgc ctgccaaagc
     2941 tgtcccgccg atcgcgttac gtcgcatgct cgaagccaac cttgccattg cgaaggcgct
     3001 taatgtgccc ttgctcgtcc gggcagataa gagctatgta cagacgctgg aggcattgtt
     3061 cgtcgccatc agccctgagc aggaatataa gctcgaagtt ctgatggatc aacttaagcc
     3121 ggaacgcttg gagcacgaag ccgtcagtga ctttatcatt ttgccggggt ttggctcccg
     3181 caagcgcgtg caagataccc ttggaaacac ccctgagcaa atcgctaaat attttgatgg
     3241 aaatttagca ctcctgcatt tcgataagta aattatctga tcatggacga tgggcaataa
     3301 accgcgatca acggtctatt gcccatcgtc ttgtttttac ttcaggtaaa tatcgaagaa
     3361 tgcaatcgtc tgattcatgg cttgggtaaa atagttcgaa atgttgtgat tatcaccttc
     3421 gtatgtgtac aaattaacga tttgacctga ttgcctgccc aactctgcca accgaatcga
     3481 aaactctacc ggcacatctt catctgcagt gccatgatgc aattccaatg gaccggatag
     3541 gtcagtaagg taggatgtcg cagagattga gtcccagaag gcggggtttt gctcgggtgt
     3601 gccgtattgt tcgatccatg ctgatcgcca tccacgcccg gtagagctag gtgacggggt
     3661 gaaggcaccc gtccggcgcc agttatacaa caggtctgag tatgaagcga cgacccccgc
     3721 ccagatcaca ccggctttta catcattcga gatgaccatg gcgcgcaggg ttaaatatcc
     3781 gcccatggaa tgcccccaca taccgatctt ctcagggttt acatctgggt gctgtttgag
     3841 agaggcgagg gcattcatca catctacttg gtagccgggg tcgccatagg caccagtggc
     3901 ttccccttcg gactggtcgt gcccgcgata gtcaattcgg aagaccacat accctgcccg
     3961 agcgagttga tcgacatatg caatatagcg ctctgttgtt ttgtatacat ctggcggaat
     4021 gtaaccatga ttgaagacga tggcagggaa cccttcttca ggtttttcac cattcggaat
     4081 ggtgagcaag gcgtaaatct tcaacccttc tgaaagatag taaacataat atcggcgata
     4141 attaatgccg cggtctagct ctttgacgat ggtgatttcg ctgccgggat attcctgatt
     4201 tcgcaacgcg atgatgctca tgggatgtgg aacgggcgtg gctgttggag ccggtgtgaa
     4261 tgtagctgtg ggctcgagtg tggaggtttg cgtgggcgta tccgttactg ggattggcgt
     4321 atgggtaggc atgggagtcg cttcggtgca ggctgtgatc gccaccaaaa acgcgaggaa
     4381 taccactaaa gttttcttca t
//
LOCUS       DAOQFT010000155         4399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10630_length_4399_cov_7.066529, whole genome shotgun sequence.
ACCESSION   DAOQFT010000155 DAOQFT010000000
VERSION     DAOQFT010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10630_length_4399_cov_7.066529"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1940
                     /gene="gyrB"
                     /locus_tag="PLR93_05275"
     CDS             9..1940
                     /gene="gyrB"
                     /locus_tag="PLR93_05275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNH26408.1"
                     /translation="MEDFVAENNYDVSSIQALEGIEHVRKRPGMYVGGTDIKALHHLV
                     YEVVDNAIDEALAGFCTRIDVTIHKDSSVSVKDNGRGIPVGPHPSKKDANGKPMETVD
                     VVMTVIGAGGKFGGGGYKVSGGLHGVGISAVNALSEWTTTEIKRDGKLWKQEYKRGVP
                     QGKIQQLGKVDKDDTGTTQTFKFDKQIFTEDIDFRFDTLVQRFREMSFVTRGITLKFV
                     DERADREMTFHFEGGITSFVRYLNRNRENLHSVVHVEKEVENIGIEAAIQYTDAYTES
                     VYSFANTINTIDGGTHLTGLRSSLTRVINDYARKSGLLKDADPNFSGDDTREGLTAIV
                     SVKHPGPQFESQTKVKLMNPEVQTYVAQVVGETFMTFLEENPQAAKAIIAKCLTSARA
                     RDAARKARDLVIRKSALESLTLPGKLADCSERDTSKTELYIVEGDSAGGSAKQGRDRH
                     FQAILPLRGKILNTERARLDKILGNNEVKALISALGTGIGDNFDLEGLRYGRVIIMTD
                     ADVDGSHIRTLLLTFFFRYMPQLIEDGHLYIAQPPLYRIAHKNNVKYVYSDKEKDKAL
                     KELGEKASLQRYKGLGEMNPDQLWDTTMNPSNRTLLLVTVDDAAEADRTFDMLMGDAV
                     DPRKKFIQTHAKSVRNLDI"
     gene            complement(1995..2549)
                     /locus_tag="PLR93_05280"
     CDS             complement(1995..2549)
                     /locus_tag="PLR93_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH26409.1"
                     /translation="MISSDELNPLILSAFTKQITWLEASQKYSLDEFIDTFLNTRAHT
                     LELLDGLTDAQAAFASTVHPFWSISESITHLVYSQGFYHNQLLDIATSQLPHVVEAAR
                     GFGEGAQQHVSVQDLKVSLNAATERIHTVIDSTRNSHDPNKTDASSAFGLCTYKTWIL
                     LLLAHEVDHLRQIAAMRTVAKTNV"
     gene            2655..3596
                     /locus_tag="PLR93_05285"
     CDS             2655..3596
                     /locus_tag="PLR93_05285"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNH26410.1"
                     /translation="MKLTTNNIIIIFGSLILCMLALIPITVLAMPLLETNQPDTQATM
                     QAMVTQTVIALTLNAPTQTPLPPTATPVPATNTPVPTVTTLPTVSYCDWMAFVTDISV
                     PDGTNFSAGETFVKTWRIKNRGTCTWTPDYTLVFNGGDQLGSTTSVRLPGYVSPGQYI
                     DVSITFTAPNNPGSYRGYWIMRNTSGALFGTGDKANAPIYVDIRVRQSDPLPHGTISG
                     NLCYPSEFNPRMTLYIENANTGERIQFAITENQYVYEVLVPAGRYYAYAWAPSYSLEG
                     AYAHDNGLMKSFFVESGKETPFINLCNWGPYGHAKGE"
     gene            complement(3648..>4399)
                     /locus_tag="PLR93_05290"
     CDS             complement(3648..>4399)
                     /locus_tag="PLR93_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNH26411.1"
                     /translation="MGVEAFNYGLRDFTPSTYSKTVMQGSEIISFKTLSEAFINVKVR
                     LEKQTSPTYIHLYFDKIDAVAHEYGPNSAQAEAEIETFLLMMENYFDQIFSSKKRVLF
                     LMTADHGMCEVDPKTTVYLNTHKDFSGVEKFIRSNKRGHLIVPAGSARDMFMYIKTDM
                     VDEAQDFLAKRLEGKADVVKTETLISEGYFGTEISQRFLERVGNVVILPYRYESVWWY
                     EKDKFEQRFRGHHGGLTPQEMETVLYSMEIG"
BASE COUNT         1121 a         1060 c         1096 g         1122 t
ORIGIN      
        1 gcaggtattt ggaggatttt gtggctgaaa acaattatga tgtaagttcg atccaagcgc
       61 tggaaggtat tgaacacgtt cgtaaacgcc ccggtatgta cgtgggtggt acggatatta
      121 aggcgttgca tcacctcgtt tatgaagtgg tggataatgc catcgatgaa gcgctggctg
      181 gtttttgtac acgcattgac gtgaccattc ataaagatag cagcgtctcc gtcaaagata
      241 atggacgtgg tattccggtg ggtccgcatc cttccaagaa agatgccaac ggcaaaccca
      301 tggaaaccgt ggatgtggtg atgaccgtca tcggcgccgg tggtaaattc ggcggcggcg
      361 gatataaagt ttctggtggt ttgcatggtg tgggtatcag tgcggttaac gctctttctg
      421 agtggacaac cactgaaatc aagcgtgatg gaaaactttg gaaacaggaa tacaaacgcg
      481 gtgtgccaca gggcaagatt caacagctcg gcaaagtgga taaagatgac accggcacca
      541 cccaaacgtt taaattcgat aagcagatct tcactgaaga tatagacttc cgctttgata
      601 cgctcgttca gcgcttccgc gaaatgtcct ttgtgacgcg cggcatcacc ctcaagtttg
      661 tggatgaacg cgctgaccgt gaaatgacct tccactttga aggcggtatt acttcattcg
      721 tccgctattt gaaccgcaac cgcgaaaact tgcactctgt ggtgcatgtt gaaaaggaag
      781 tggaaaatat tggcattgaa gctgctatcc aatataccga tgcttatact gagtctgtgt
      841 actcttttgc gaacaccatc aacactattg acggtggtac acacctcact ggtttgcgct
      901 cttccctaac tcgtgtgatc aatgactatg ctcgtaagag cggtttgctc aaagatgcgg
      961 acccgaactt ctctggcgat gacacccgtg aaggtctcac tgctattgtg tcggtcaaac
     1021 atcctggtcc gcagtttgaa tcgcagacca aagtgaagtt gatgaacccc gaagtgcaga
     1081 cctatgtggc tcaggtcgtc ggcgaaacct ttatgacctt ccttgaggaa aacccgcagg
     1141 ctgccaaggc gatcattgcc aaatgtctca cctcggcgcg tgcgcgtgac gctgcccgca
     1201 aggcgcgtga cctcgtcatc cgtaaatctg cgcttgagtc gttgaccctg cccggtaaac
     1261 tggcagactg ctcggaacgc gatacatcaa aaactgaact ctacatcgta gaaggtgact
     1321 cggcaggtgg ctctgctaaa cagggacgtg accgccactt ccaggcgatc cttccgttgc
     1381 gcggtaagat cctcaacacc gaacgtgccc gcctcgataa gattctcggt aacaacgaag
     1441 tcaaggcgct tatctctgcg ctgggaactg gcattggcga taacttcgac ctcgaaggtc
     1501 tgcgctacgg acgcgtcatc atcatgaccg atgctgacgt agacggtagt catatccgta
     1561 cacttctgtt gaccttcttc ttccgctata tgccgcagtt gatcgaagac ggtcacttgt
     1621 atattgccca gccgcctctt tatcgcatcg ctcacaagaa taacgttaag tatgtgtata
     1681 gcgacaaaga aaaagacaag gcgctcaaag aactcggcga gaaagccagc cttcagcgtt
     1741 ataaaggtct cggtgaaatg aacccagacc agttatggga tacgacaatg aatccatcga
     1801 accgcacgtt gttgttggtg acggtggatg atgctgctga agcggaccgt acctttgaca
     1861 tgctgatggg tgacgccgtg gacccacgca agaagttcat ccaaacccat gccaagagcg
     1921 tgagaaattt ggatatttag tttcgttgaa taaaaaacag cccgcgaatt cgcgggctgt
     1981 ttttttattc atgtttagac gtttgtcttt gctactgttc tcatcgccgc gatctgacgg
     2041 agatgatcca cttcatgtgc cagcaaaagc agaatccatg ttttataagt gcacaaccca
     2101 aatgccgagc tggcatctgt tttattgggg tcatgtgaat tgcgagtcga atcgatcacc
     2161 gtgtggatcc gctcggtagc ggcattcagg ctcaccttta agtcttgaac cgaaacatgt
     2221 tgttgggcgc cttctccaaa tccacgggcg gcttctacaa catgcggcaa ctgactggtg
     2281 gcaatatcca gcaattggtt gtgataaaac ccctgcgagt acaccaagtg tgtaatggat
     2341 tcgctgatgc tccagaaagg gtgaacggta gatgcaaaag ccgcttgcgc gtcggttagc
     2401 ccatcgagca actccaacgt atgggcgcgg gtgttgagga aggtatcgat aaattcatcc
     2461 aaagaatatt tttgagaggc ttccaaccaa gtgatttgct tggtaaatgc ggaaagaata
     2521 agagggttta attcatcgga agagatcata ggttggattt tatcttattt tttttagtac
     2581 tttggtactg gaagtgacgg gaacaatttg tgccgggcaa acgttctagg tgggcaaatg
     2641 aaaaagagga ctttatgaaa cttacgacca ataacatcat tattattttc ggtagcctga
     2701 tcttatgtat gctggcgctg attccgatta ccgtgcttgc catgcctttg ttagagacaa
     2761 accaaccaga cacgcaagcc acgatgcagg cgatggtgac ccaaaccgtg attgccttaa
     2821 cgcttaacgc cccgacacaa accccgcttc ctcctacggc gacgcctgtt cctgccacga
     2881 atacacccgt gcctacggtg acaacccttc ctaccgtcag ctactgtgac tggatggcat
     2941 tcgtcacaga catctctgta cccgacggga cgaatttttc tgccggcgag acctttgtca
     3001 aaacatggcg catcaagaat cgcggcacat gcacttggac gccagattac acccttgtgt
     3061 tcaacggcgg tgaccagtta ggttccacaa cttcggtgcg tttgcctggg tatgtatctc
     3121 cggggcaata cattgatgtg tccattacat tcacagcgcc aaataacccc ggctcgtatc
     3181 gcggatattg gatcatgcgc aacacatctg gcgcgctctt cggcaccggt gataaagcca
     3241 atgcccccat ctatgtggac attcgtgtgc gtcagagtga cccgctgcct catggcacta
     3301 ttagtggaaa cctatgttac ccgagtgagt ttaatccgcg catgacgctt tacatcgaga
     3361 atgccaatac aggcgagcgc attcagttcg cgattactga gaaccagtat gtgtacgaag
     3421 tgctagtgcc tgccggacgc tactacgctt atgcttgggc tccgagctat agcttggaag
     3481 gagcatatgc tcacgataat gggctgatga aatccttctt tgtggaaagc ggcaaggaaa
     3541 caccgtttat caacctgtgt aactggggtc cgtacggaca cgcgaaaggc gaataaaaaa
     3601 tcaaaaaagc gacctgtaat aggtcgcttt tttgattgag tatattgtta tccaatttcc
     3661 atggaataca agaccgtttc catctcttgc ggagttagcc cgccatggtg cccacgaaag
     3721 cgttgctcaa atttatcttt ctcataccac catactgatt catagcgata cggcaaaatg
     3781 accacattcc caacccgctc taaaaatcgc tgagaaatct ctgtcccaaa atacccttca
     3841 gagatgaggg tttcggtctt gaccacatcg gctttgcctt ccaaacgttt tgcgaggaag
     3901 tcttgagctt cgtctaccat gtcggtcttg atgtacatga acatatcgcg tgcagaccct
     3961 gccgggacga tcaaatgtcc gcgtttgttt gagcggataa acttttcgac accagagaaa
     4021 tctttgtgag tgttgagata caccgtggtt ttaggatcca cttcacacat gccatgatcg
     4081 gcagtcatca aaaaaaggac gcgtttcttg ctgctgaata tttggtcgaa gtaattctcc
     4141 atcatcagca ggaaggtttc gatctcggct tcagcttgag cggagttggg tccgtattca
     4201 tgagcgacag cgtcaatctt gtcaaaataa agatggatgt atgtggggga ggtctgtttt
     4261 tcgagccgta ccttcacgtt gatgaatgcc tcagaaagag tcttgaacga aataatctct
     4321 gagccttgca tcacggtttt ggaatatgta gagggtgtga aatcacgcaa gccataattg
     4381 aaggcttcca cgcccattt
//
LOCUS       DAOQFT010000156         4381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10708_length_4381_cov_5.625982, whole genome shotgun sequence.
ACCESSION   DAOQFT010000156 DAOQFT010000000
VERSION     DAOQFT010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10708_length_4381_cov_5.625982"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLR93_05295"
     CDS             complement(<1..334)
                     /locus_tag="PLR93_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNH26412.1"
                     /translation="MFRIFRRKEDKFQKLIEQQAEISFEGLKLLVKYLETSAPDVAEE
                     LSFKEKEADEVRRILIDELNRTFVTPFDREDIFSLSRSIDDVVDYADTTVVEMVILKV
                     SPTPYMQRI"
     gene            complement(360..1796)
                     /locus_tag="PLR93_05300"
     CDS             complement(360..1796)
                     /locus_tag="PLR93_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNH26413.1"
                     /translation="MSFAALLTQEQVEKIHDASLEILEDVGLKVRYEPARELFKKHGC
                     MVEDERVKFPRAVVEKYRKLVPSSFTFHARDPKFDKTIPQDSPVIVTASSAPDIIDPV
                     TGKERRAESRDIAQIAHLINELPGYDIFSISTLADDAPADQFTVSRLYPSIKYCLKPI
                     RVTTTNMKDTMSIMEMAYIVAGGEAAYKEHPFLTHHYCPVVSPLTMDHLSTENVMFFA
                     QQGLPVYPTIVPNAGLTSPMSMAGSLVQGNAEFLSALMLMQMTKEGTPTIYATLGTVA
                     DMRSGAYTSGAIECGMLHMAFAQMARFYDVPAGGYVGLTNSKLNDAQAGYETGMSGIA
                     GVLGGMDMFNIGGLIDALKTFDFAKAVIDDEIAQMMKRMKRGVSFSDEDLGVNLIKEI
                     GPGGSFITAKHTTSRMKTEAVMTKIADRDARSIWEKKGATDTHAKALKKAKDVMASNT
                     TPLISPEVDEKIRAAFPGLVPGVLEPIP"
     gene            complement(1820..2911)
                     /locus_tag="PLR93_05305"
     CDS             complement(1820..2911)
                     /locus_tag="PLR93_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH26414.1"
                     /translation="MAKKNSSPAYIGFGMLTPVYIMSLDQLPKLNTGAIVHQVSDYVY
                     DDAAIIACNLSQWNVPSAMIGTAVGKDLLGKYVADKLKELGVKGEVRFTAKYKTPLEV
                     NVSDKKGARTYFWQRSKEILDTLSTADLSLIKSAKLLYVDWYDGEHIVRAMAEAKKHN
                     VPVFLNFEHGHQNPDLLKKYAGMVTVCQAVTDAAQIGKRQAMLGTARKLIKSGIQTAI
                     ITMASQGCMVVQGDEIIRAYAPKVKAVDASGAGATFSSGYIYGYLNGWSLEDTVRFAI
                     AAASLKVTRSGLDMFPVSEIKGLARMVRIERMVYRGDQFRTIRKLLTLPENSPLVNNA
                     LVKESQKLAERILPKKKIERKKIKRSLVE"
     gene            complement(2930..>4381)
                     /locus_tag="PLR93_05310"
     CDS             complement(2930..>4381)
                     /locus_tag="PLR93_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNH26415.1"
                     /translation="PMPGVKGFWMMAGMSLNGYGGAGGMGKLIAEWIMDGEAPMDVYG
                     YRATRFGNYYSDFNYAAERTVEAVKYYYRLKFPHDESEAARPHRTSPVHYRLMENGAV
                     FGEKFGWERVNYFNPGNEWRRMGEDQREWGWTKPPYFERMRVEHIATRERVALFDLTS
                     FGKIEVKGPGALPLLQRLTSSNIDKPVGSAVYTQFLNRNGGIESDLTVTRLGEEYFWV
                     ITGSGFIANDLARIQMNVDEKDGEVSIRDITQEHACLALWGPKARDVLKKITSSDVSN
                     EGHPYLTTKPIQINGVKVIAQRVSYAGELGWELYIPNNRATMVWDMLVESGKEFGMEL
                     GGYKVLDPLRLEKGFKYFTTDITPTTTPYEAGLGFCVDLNKGDFIGRDALVKQKADGV
                     TRKLCTLVLTDTDEFVQIYGGEAVHHEGKVVTRIRSGGYGFTVKKNILYAYLPVDLAV
                     KGTRFTIELIEGNLAAEVTANVLYDPKSEKLKA"
BASE COUNT         1137 a         1165 c          925 g         1154 t
ORIGIN      
        1 caattcgctg catataaggt gttgggctga cttttagaat caccatttct acaacagttg
       61 tatcggcata atcaaccaca tcatcgatcg aacgagagag cgaaaagata tcttcacggt
      121 caaacggtgt gacaaatgtg cggttcagtt catcgatcaa gatgcgacga acttcatccg
      181 cctctttctc tttgaacgac agttcttcag ctacatctgg agccgacgtt tcaagatatt
      241 tcaccaataa cttcaagcct tcaaacgata tttccgcttg ttgctcaatc agtttttgga
      301 atttatcttc cttgcgccta aaaatacgaa acattcaaat ccttctttta ttccttgtat
      361 tacgggatag gctctaacac cccgggcact aagccaggga atgcagcgcg aatcttttca
      421 tccacttcgg gcgaaatcag aggagtggta ttggaagcca taacatcttt ggctttcttc
      481 aacgccttgg catgtgtatc cgtagcgcct ttcttctccc aaatggaacg agcatcacgg
      541 tcagcgattt tggtcatcac cgcttcggtc ttcatgcggc tggtagtgtg tttggctgtg
      601 ataaaggagc cgccaggacc gatctctttg atgagattga ctcccaaatc ttcatcgcta
      661 aagctgactc cacgtttcat acgcttcatc atctgtgcaa tttcatcatc aatcactgct
      721 ttggcaaaat cgaaggtctt caatgcatcg atcaaaccgc caatgttgaa catatccata
      781 ccgccgagca ctcccgcaat gccagacata ccggtttcat accctgcctg agcatcgttc
      841 agtttggagt tcgtcaagcc aacatatccg cccgcaggca catcataaaa acgcgccatc
      901 tgagcaaatg ccatatgcaa catgccacat tcaatcgcgc cagaggtata tgcacccgaa
      961 cgcatatcag ccactgtacc cagtgttgca tagatcgtcg gtgtgccttc ctttgtcatc
     1021 tgcatcaaca tcagcgcaga taaaaattca gcattacctt gcaccaaact accagccata
     1081 gacatgggcg atgtaagacc ggcattcggc acaatggttg ggtaaacagg cagcccttgt
     1141 tgtgcaaaga acatcacatt ctccgtcgag agatggtcca ttgtcaacgg cgaaacgacc
     1201 gggcaatagt gatgcgtaag gaacggatgc tccttatatg ccgcttcccc accagccaca
     1261 atgtatgcca tttccatgat gctcatggtg tctttcatgt tggtcgtagt cacacggatc
     1321 ggtttaagac aatacttgat ggacggatac aagcgcgata ccgtgaattg atccgcgggt
     1381 gcgtcatctg ccaacgtgga aatggagaag atatcgtatc cgggcaattc gttgatgagg
     1441 tgggcgatct gcgctatatc tctggattcg gcgcgtcttt ctttgccggt cactggatca
     1501 ataatatcgg gcgcggaact cgctgtaaca atgaccgggc tatcctgcgg aatggtctta
     1561 tcaaatttcg ggtcacgtgc gtgaaaggta aaagatgacg gcaccaactt gcggtatttc
     1621 tccacaaccg cgcgcgggaa tttcacacgc tcatcttcga ccatacagcc atgtttcttg
     1681 aacaactcgc gagcaggttc atagcggacc tttaagccaa catcttccaa aatctctaac
     1741 gaagcatcgt gaatcttttc gacttgttct tgcgtaagca aagcagcaaa tgacatggtt
     1801 cacatcctta taaaataaat tattcaacca gtgagcgttt gatcttttta cgctctattt
     1861 tcttcttagg caaaattctt tccgccagct tctgactttc tttcacgagc gcattgttaa
     1921 ccaatggact gttctctgga agggtcaaca acttacgaat agtacggaat tggtctccgc
     1981 gatacaccat gcgttcaata cgaaccatgc gagccaatcc tttgatttca gatactggga
     2041 acatatccaa cccagaacgg gtcactttta aagacgctgc agcaattgca aaacgaaccg
     2101 tgtcttccaa gctccagcca ttgagataac cataaatata gccagatgaa aatgtggcgc
     2161 ccgctcctga ggcgtcaaca gccttaacct tgggcgcata ggcgcggata atttcatcac
     2221 cttgcaccac catacacccc tgacttgcca tagtgatgat agctgtttga ataccagatt
     2281 taatgagctt gcgcgctgtg cctaacatag cttgacgttt gccaatctgc gccgcatcgg
     2341 tcactgcctg acagaccgtg accatgcccg catatttttt gagcaggtct gggttctgat
     2401 gcccatgctc aaagttcaaa aagacgggca cattatgttt cttggcttct gccatggcac
     2461 ggacgatatg ctcgccatcg taccagtcca catacaatag ttttgcagat ttgatcagtg
     2521 acaaatccgc tgtggacaat gtatcaagaa tttcctttga acgttgccaa aaatacgtgc
     2581 gcgcgccttt tttgtcagag acgttcactt ccaaaggcgt tttatatttc gccgtaaatc
     2641 gcacttcgcc ttttacgccc aattctttca atttatctgc gacatatttt ccaagcaagt
     2701 ctttacccac cgctgttcca atcatggcgg acggtacatt ccactggcta aggttgcaag
     2761 caatgatggc agcatcgtca tacacataat cgctgacctg atgcacgatg gcacctgtat
     2821 ttaacttggg caattgatcc agcgacataa tgtacacagg cgtcaacatg ccaaagccaa
     2881 tgtaagctgg agaggaattt ttcttagcca taattagagg gtaatcaatt tacgctttca
     2941 gcttctcact cttcgggtcg tacaagacat tggcagtcac ttcagcagcg agattgccct
     3001 cgatcaattc aatggtgaac cgagtgcctt taactgccaa atccactggc aggtaggcat
     3061 acaaaatatt ctttttcacg gtaaagccgt agccgccact gcggatacga gtgacaactt
     3121 tcccttcgtg atgaaccgct tcaccgccgt agatctgcac aaattcatcg gtatcggtca
     3181 ataccaaagt acagagtttc cgcgttactc catctgcttt ttgcttgacc aacgcatcgc
     3241 gcccgatgaa gtcacccttg ttcaagtcca cgcaaaagcc aagaccagct tcataaggtg
     3301 tggttgttgg ggtaatatcg gtcgtgaaat acttaaagcc tttttccaaa cgtagcgggt
     3361 ccagcacttt atagccgccg agttccatgc caaattcttt accagattca acaagcatat
     3421 cccataccat ggtggcacga ttgttcggaa tgtacaactc ccagcccaat tcgccagcat
     3481 agcttactcg ttgtgcaatc actttcacgc cgttaatttg aatcggcttt gtggtcagat
     3541 aaggatgccc ttcattagac acgtcactgc tagtaatctt ctttaataca tcacgcgcct
     3601 tgggtcccca caaggcaaga caggcatgtt cctgcgtaat atcacgaatg gatacctcgc
     3661 catctttttc atccacattc atttgaatgc gggcaaggtc gttggcaata aaaccagatc
     3721 ccgtgatgac ccagaagtat tcctcaccca aacgagtgac cgtcaaatca gactcaatac
     3781 cgccattgcg attcaagaat tgggtataca ccgcgctgcc aacgggctta tcaatattac
     3841 tcgacgttaa gcgttgcaac aaaggcaatg caccgggtcc tttgacttcg atcttgccaa
     3901 aagaggttag gtcaaacagt gccacacgct cacgggttgc aatatgttcc actcgcatcc
     3961 gttcgaagta tggaggtttc gtccaccccc actcgcgttg atcttcgccc atacgacgcc
     4021 actcattacc tgggttgaaa taattcacgc gctcccagcc aaacttctcg ccaaaaaccg
     4081 cgccattttc catcaaacga taatgcaccg ggctggtgcg atgcggacgt gccgcttccg
     4141 attcatcgtg cgggaatttc aaacgatagt aatactttac ggcttccacc gttcgttcgg
     4201 cggcatagtt gaaatcagaa tagtaattgc caaagcgtgt ggcacgatag ccatacacat
     4261 ccatcggtgc ttcaccatcc atgatccact cggcgatcag tttgcccatg ccgcctgcac
     4321 caccgtagcc attgagcgac atacccgcca tcatccaaaa acctttcacg ccgggcatcg
     4381 g
//
LOCUS       DAOQFT010000157         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10753_length_4371_cov_7.363763, whole genome shotgun sequence.
ACCESSION   DAOQFT010000157 DAOQFT010000000
VERSION     DAOQFT010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10753_length_4371_cov_7.363763"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLR93_05315"
     CDS             <1..698
                     /locus_tag="PLR93_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH26416.1"
                     /translation="GYLPESVPLYYDMVVSEYLKYMGELRRIPNVDDRVDEVLDMVGL
                     FDRADGYIGNLSKGMKQRVGLAQALLHRPEVLILDEPTIGLDPGQVVEVRELIREIGK
                     ERTVLLSTHLLNEAQNLCDRVLIINKGKIVAEDTTENLQARLLGAERVVIRVRGEAEE
                     LADSIKSIKGVRKVETKDDGAVEFEFASGKDLRPEVAKQVIKDGYDLLELRPLGMSLE
                     EIFLELTGNEGKK"
     gene            703..1446
                     /locus_tag="PLR93_05320"
     CDS             703..1446
                     /locus_tag="PLR93_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26417.1"
                     /translation="MNNIWTIAKREYNNYFNGPLAYVVMLAILLPLGIYFSLILFYSS
                     QQGLYGGSAAPDITPVNGLFAFLMIFLSPALTMRLLSDEARMGTLELLLTAPVRDIEL
                     VVGKWLGAMLFILTLLGTTLIYPLVLNNFVDPGIDWMVVLSSYIALILLCGTMLALGV
                     GISAIFTNQFAAFFVTLGLIFFLYFMIGLPAGLLPQSGEVFNYLSIQNHFDQMNSGTL
                     TLGSIVYNLSLTVFGLFAGFTAIETRRWR"
     gene            1446..3068
                     /locus_tag="PLR93_05325"
     CDS             1446..3068
                     /locus_tag="PLR93_05325"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNH26418.1"
                     /translation="MAGKKKNPNARYAVIALAVSLVACISTGLIGSANLLSNIGMFPL
                     SDEIQNGVNIALQVSIGLLIIALAVAAILNPDPVRRFFTGRQARYGSNSLILTIAVIG
                     ILIAGNYIVFKNTDLLGSPYDFTEDKSNTLAPETLQILAELKEPVVATGFFSTNSSTA
                     SAEELLAKFKNNSDGKFTYNFVNPDVDPVAAREAGITGDGKIMLQMGETKEIATFATE
                     DELARTLLRLMSPGARTVYFLQGHGEISLESGNELAYSVVQSTLEAKNYTVSTLNLLT
                     TREIPADALAIIVAGPQKPVSAEEVDLLKAYVDKGGSLVILEDPIFFTKFGDAADPLA
                     EYLVNDWGIVLNNDVVIDGVNQQNPLAAISSLYNPDHPITQNLTENLFVIMPQARSIS
                     VSGEKENITQTWLISTTENSWGETSPLVENENPQFDPATDVQGPLYLALAAENSATTG
                     RVVVFGNSLFAIDGNFDVYGNGNFFINSVDWAAEQEDLLNLTTRPRTERIFTPPVETW
                     RFLLLVLVIVIIIPGMVVFFGVSAWLARRKRG"
     assembly_gap    3104..3203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3227..3949
                     /locus_tag="PLR93_05330"
     CDS             3227..3949
                     /locus_tag="PLR93_05330"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNH26419.1"
                     /translation="MATKSTKTTKSTKTTKTTKPRASRAQSTPKVSKPAVRTGTWVAL
                     IAFIAVIATLIYMNRKAETEAAATETPAAEETFIFSSESVVSSIEVKPAEGNAVKVER
                     NEEKTWVLSQPEKAEANQAASEAAATQIGALSIVTTIEGNKDPSIFGLDAPVYTITVT
                     FDDGKKGVLEVGDTTPTSTGYYVRLDKDKVYVVAMDGIDALTSLTVAPPYLNTPTPVP
                     TATSTPLPTDTPATTTEATPTP"
     gene            4130..>4371
                     /locus_tag="PLR93_05335"
     CDS             4130..>4371
                     /locus_tag="PLR93_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNH26420.1"
                     /translation="MDVDKILISEDDEEEYSAIARLIELGRQKSFVTLDDILHFFPEA
                     EQDVEQLEEAFSALLSAGIPFVEESMTPEPTEDELVA"
BASE COUNT         1064 a         1135 c         1007 g         1065 t
ORIGIN      
        1 tcggctattt gccggagtct gtgccgctgt actacgacat ggtcgtttct gaatacttaa
       61 agtatatggg cgaacttcgc cgcatcccga atgtggatga ccgtgtggat gaagtgttgg
      121 atatggtcgg cttgtttgac cgtgccgacg ggtacattgg caacctgtcc aaaggtatga
      181 aacagcgtgt aggcttggct caggcgcttt tgcaccgccc cgaagtgctc atccttgatg
      241 agccgacaat tggtctcgac cctgggcagg tggtagaagt ccgcgaatta atccgcgaga
      301 ttggtaaaga gcgcacggtg ctcctctcca ctcacctttt gaatgaagcg caaaacctct
      361 gcgaccgcgt cttgatcatc aacaagggca agatcgtcgc cgaagacacc accgaaaact
      421 tgcaggctcg cttgctcggt gccgaacggg ttgtgatccg tgtacgtggc gaagccgaag
      481 agcttgcaga ctcgatcaag agcattaagg gtgtccgcaa ggtggaaacc aaagacgacg
      541 gtgctgtgga attcgagttt gcctccggca aggatttacg ccccgaagtt gccaaacagg
      601 ttattaaaga tgggtacgac cttttggaac tccgcccgtt gggcatgagc ctcgaagaaa
      661 tcttcctcga actcactgga aacgaaggga agaaataatc ccatgaacaa tatttggacc
      721 atcgcaaaac gcgaatacaa caactacttt aacggaccgc tcgcctatgt tgtgatgctg
      781 gcgatccttt tgccacttgg catttacttc agtttgatcc ttttctacag cagccagcaa
      841 ggtttatatg gcggcagcgc tgcccccgat atcacccccg tcaacgggtt gttcgctttc
      901 cttatgatct tcctcagccc ggcgcttact atgcgtttgc tctctgacga agctcgtatg
      961 ggaactctcg aacttttgct gaccgccccc gtccgcgata ttgagctggt ggttggcaag
     1021 tggctgggcg ccatgttgtt catcctcacc ttgcttggca cgaccttgat ctatccgctc
     1081 gtgctgaaca acttcgttga ccccggcatc gactggatgg tggtgctttc ttcttatatc
     1141 gctttgatcc tgctttgcgg caccatgttg gctttggggg ttggcatctc tgccattttc
     1201 acgaaccagt ttgctgcctt cttcgtgacc ctcggcttga tcttcttctt gtacttcatg
     1261 atcggtctgc ctgcagggtt gctcccccaa agcggtgaag tcttcaacta cttgagcatt
     1321 caaaatcact ttgaccaaat gaattccggc acactaacac tcggcagcat cgtctataac
     1381 ttgagcctga cggtgttcgg tctcttcgcc ggttttaccg caattgaaac tcggagatgg
     1441 cgataatggc tgggaaaaag aaaaatccaa acgcaagata cgcagtcatc gcgctggcag
     1501 tttctctggt cgcatgtatt tcgaccggtt tgatcggctc tgcgaatctg ctttctaaca
     1561 ttggcatgtt cccgctttct gacgaaattc agaacggcgt gaacattgct ttacaagtca
     1621 gcattggttt attgatcatt gctctggcag tcgccgcgat tttgaacccg gatcccgtcc
     1681 gccgcttctt taccggacgc caagctcgct atggttcaaa ttccttgatc ctcacgattg
     1741 ccgtgatcgg tatcctcatt gctgggaact acattgtctt caagaacacc gacttgctcg
     1801 gttctcctta cgattttacc gaagacaaga gcaataccct tgctccagaa accctgcaaa
     1861 ttttggctga attaaaagaa cctgtcgtag ctacaggctt tttctcgaca aattcaagca
     1921 cagccagcgc agaggaattg ctcgctaaat tcaagaataa cagcgacggc aagtttacct
     1981 acaacttcgt caacccagat gttgaccccg ttgccgctcg cgaagctggt attacaggcg
     2041 acggcaagat catgttgcaa atgggcgaaa caaaagaaat cgctaccttt gctacagaag
     2101 atgaacttgc gcgcaccctt ttgcgcctta tgagccccgg cgcacgcacg gtctatttct
     2161 tgcagggaca tggcgagatc agccttgaat ctggcaacga attagcctac agcgttgtac
     2221 aaagtaccct tgaggcaaag aattacaccg tcagcacgct taatctgttg acgactcgcg
     2281 aaatccctgc agacgctttg gcaattatcg ttgcgggtcc gcaaaaacct gtcagcgcag
     2341 aagaagtgga cttgctcaaa gcctatgtgg ataagggcgg ttccttggtc attctcgaag
     2401 acccgatctt cttcaccaag tttggtgatg ccgcagaccc cttggctgaa tacctcgtca
     2461 atgattgggg cattgttctc aataatgacg ttgtgatcga tggtgtcaac cagcaaaatc
     2521 cgctggcggc gatctcttct ttgtacaatc ccgatcaccc catcacccaa aacctgaccg
     2581 aaaacctttt cgtcatcatg ccacaagctc gcagcatcag tgtttctggt gaaaaagaaa
     2641 atatcaccca aacttggctc atcagcacca cagaaaactc ctggggcgaa acctctccct
     2701 tggtagaaaa cgagaatcca caattcgacc ccgcaactga cgttcaaggt cctttgtacc
     2761 tcgcccttgc tgcggagaat tctgcgacga ccggtcgtgt ggttgtgttc ggtaactcac
     2821 tttttgctat tgacggaaac ttcgatgtgt atggcaacgg caacttcttc atcaattctg
     2881 tagattgggc tgccgaacag gaagaccttc tgaacctcac cacccgtcca cgcaccgaac
     2941 gcatcttcac acctccggta gaaacctggc gcttcctgct cttggttctc gtgatcgtga
     3001 ttatcatccc cggtatggtt gttttctttg gcgtgtctgc atggcttgcc cgtcgtaaac
     3061 gaggttaatt catggctacg aaatcgacca aaacaacaaa atcnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnncccgtcg taaacgaggt taattcatgg ctacgaaatc
     3241 gaccaaaaca acaaaatcta caaaaacaac caagaccacc aaaccacgcg cgtctcgtgc
     3301 gcaaagcacc ccaaaggtct ccaaacccgc cgtccgcacc ggaacatggg ttgcgctgat
     3361 cgctttcatc gctgtgattg ccactttgat ctacatgaac cgcaaggcag aaacagaagc
     3421 cgcagctaca gaaaccccgg cggctgaaga gacattcatc ttctcaagcg aaagcgttgt
     3481 cagcagtatt gaggttaagc ctgctgaagg taacgcagtt aaggttgaac gaaacgaaga
     3541 gaaaacctgg gtgttatctc agcctgaaaa agcagaagca aatcaggctg cgtctgaggc
     3601 ggctgccact caaattggtg cgctgagcat tgtcaccacc attgaaggca ataaagaccc
     3661 ctccatcttc ggtttggatg ccccggttta caccatcaca gtcacctttg atgatggtaa
     3721 aaaaggtgtc ctcgaagtgg gcgataccac cccaacctcc actggttact acgtgcgttt
     3781 ggataaagat aaggtgtatg ttgtcgctat ggacggaatt gacgcgctca ccagcctgac
     3841 cgtcgccccg ccatatttga atacccccac accggttcca acggcgacat ccacgcctct
     3901 tccgaccgat acgcctgcta caacaaccga agctactcct accccctaag tttttacggc
     3961 gtcggggaat ctgtttgaaa attgaagcag attccccgac ccgattcctc caaaaagtaa
     4021 tacaaaatca ttgcaaggca tctgataagc cgaacactat tgcttttcgg ttcaaaaacg
     4081 tgtattctga aatgtaagac aaatatttgt tgaaagttgg atgatgtgaa tggatgtaga
     4141 taagatacta attagcgaag atgatgaaga agagtattct gctatagcgc gtttgatcga
     4201 actggggcgg caaaaatcct ttgtaactct cgacgacata ttgcacttct ttcccgaagc
     4261 ggaacaagac gtagaacagc ttgaagaagc gttctctgcg cttttaagcg ctggtatccc
     4321 gttcgtggaa gaaagcatga cccccgaacc caccgaagac gaattggtgg c
//
LOCUS       DAOQFT010000158         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10796_length_4360_cov_4.553542, whole genome shotgun sequence.
ACCESSION   DAOQFT010000158 DAOQFT010000000
VERSION     DAOQFT010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10796_length_4360_cov_4.553542"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLR93_05340"
     CDS             complement(<1..261)
                     /locus_tag="PLR93_05340"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26421.1"
                     /translation="MSEIFEITTLSLQVSMLATVISLVIGLPFGTWLALGKFRGRSIL
                     LSIVNTGMALPPVVVGLVVAMTLWRSGPLGDLRLIYTPWAIVI"
     gene            complement(315..1250)
                     /locus_tag="PLR93_05345"
     CDS             complement(315..1250)
                     /locus_tag="PLR93_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH26422.1"
                     /translation="MKLTTRNFLLTLLLVLSLTLAACASPAATEAPATEAPATEAAAT
                     EAPTEAAAPANPNLILATTTSTQDSGLLDVLVPMFEEQTGYTVQTVAVGTGEALKMGE
                     EGNADVLLVHAPSSEVTYMEGGHGQDRFLVMHNDFVIVGPAEDPAGIKGLGPKDAFVA
                     IFNAGAPFVSRGDDSGTHKKEVSFWQKAELDPAGQAWFFETGQGMGASLTVASEKQAY
                     ILTDRATYLANKDNLQLEILLEGNNALLNVYHVITVNPTKSDKINYDGALAFANFIIA
                     PETQAVIGEFGVDKFGAPLFVPDAGKDPAELGLDS"
     gene            complement(1255..1833)
                     /locus_tag="PLR93_05350"
     CDS             complement(1255..1833)
                     /locus_tag="PLR93_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26423.1"
                     /translation="MNGTSSLKTTSSVSQTDWSLSWVEALLLIGGGVMAVVLHRTTDL
                     SLGLPGHHGLEWMAIMILGRASSKFRGAGMLTSIGASVSTFMPGLQGDNPFGWLFYLL
                     PGLVMDFGFYYAPRFTKHIWFLVLLGGLAHATKPLGQLTMNLITGWPFGSFRYGVAYP
                     FASHFLFGMIGGLLGALVALGIHRSSKKLNQG"
     gene            complement(1784..2506)
                     /locus_tag="PLR93_05355"
     CDS             complement(1784..2506)
                     /locus_tag="PLR93_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26424.1"
                     /translation="MHILLGIDDTDNLESRGTGHRVRQLADWLAENGLAHPLGITRHQ
                     LLVDSRIPYTSHNSSACLSIETENTDNVWEAAREFLLRESAVGSDTGLCLAEWNSISE
                     DVLDWGRKAKVTVLTMPEAREIASMSKIRCEGLTGTHGGIIGALAATGLHRAGDDGRY
                     LWLPGLRELKGKYPVHELCTLGHIDRVCTLEQVELAHDSVVDVGEWIRPILKNGKATL
                     YVEEKNHEWNIISKDHIKRLSN"
     gene            complement(2589..3029)
                     /locus_tag="PLR93_05360"
     CDS             complement(2589..3029)
                     /locus_tag="PLR93_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26425.1"
                     /translation="MKRIFLLLSFAATGIMILSACAAPAERSAAPETSADTSQVLFTI
                     IRADGSTYDVTLNAVKALPLAQVQAEGKTEEGPYLKDVLALAGVTEFTEVSLSGSSNP
                     VTLTFEQVDENTILDFNNHGTMKLSSTYIPKAEWTKDVAEIVVK"
     gene            complement(3054..4031)
                     /locus_tag="PLR93_05365"
     CDS             complement(3054..4031)
                     /locus_tag="PLR93_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH26426.1"
                     /translation="MKKIVQLQAFDQIKTLSDPRRLDILRLLMAAPATLTLLARTMKQ
                     SPAWVRHHILALESAGLIEVSEIRKTGKVMEKFYCAKADALLLQEIILPKSKKPAVIF
                     SGSHDLALENVSEQLAKYVNLLSLPVGSLDGLVNLRQGLCHISGSHLLDESGEYNTPF
                     VKRLFPDRNVEIVTLAYRTQGLIIASGNPKGIKKIADIARPSVRFVNRNPGSGTRLWL
                     DAELRKQKIPVEKISGYEEQVKTHSEAANLVIQNKVDVSLGLQAAAYQNGLDFIPLFE
                     ERYDLVLPREQEENLNPLLEYIQTVNFREMLALLTGYNTSHSGEIVTAS"
BASE COUNT         1076 a         1089 c         1142 g         1053 t
ORIGIN      
        1 gatgacgatc gcccatggag tgtagatgag tcgcaagtcg ccgagagggc cgctgcgcca
       61 gagagtcatt gccaccacta accctaccac cacaggcggc agtgccatgc ctgtgttgac
      121 gatgctcaga agaatggaac gcccgcgaaa ttttccaagc gccagccagg tgccaaaagg
      181 caagccaatc acaaggctaa tgactgtagc caacatggaa acttgcaaag aaagagttgt
      241 gatctcgaag atttcactca taatctaaaa agaaatggac ggctcgtgag agccgtccag
      301 atgatacttc aactttagct atcgagccca agttctgcgg ggtctttgcc agcgtcgggc
      361 acgaagagcg gtgcgccgaa tttatctacg ccgaattcac cgatgactgc ctgagtttca
      421 ggagcgataa tgaagttggc gaatgccaga gcgccatcgt agttgatctt gtcagacttg
      481 gtggggttga cggtgatgac gtgatagacg ttcaaaagtg cgttgttacc ttcaagcaga
      541 atttccaatt gaaggttgtc tttgtttgca aggtaggtgg cgcggtcggt gaggatgtag
      601 gcttgctttt cggaagcgac agtgagtgag gcacccatgc cctgaccggt ttcaaagaac
      661 caagcctgac ctgccggatc gagttcggct ttctgccaga agctcacttc tttcttatgc
      721 gtacctgagt catcgccgcg agagacgaaa ggtgcgcccg cattgaagat ggcaacaaaa
      781 gcatccttgg gcccaagccc cttaatgcct gcagggtctt cggcgggacc gacaatgacg
      841 aagtcattgt gcatcacgag gaaacggtct tgtccgtgac caccttccat ataggtgact
      901 tcggaggaag gcgcatggac gagaagcaca tcggcgttgc cttcttcgcc catcttgagc
      961 gcttcacccg tgccgactgc gacggtctgc acggtgtaac cggtctgttc ttcgaacatg
     1021 ggcacgagca catccagcaa accagaatcc tgtgtagagg tggtggtggc gaggatgagg
     1081 ttggggttgg cgggtgcggc ggcttcggtc ggcgcttcgg tagcggcagc ctcggtggct
     1141 ggcgcttcgg tcgcaggagc ctctgtcgct gcgggggagg cgcaggctgc taatgtcaga
     1201 gaaaggacga gcaacaacgt caaaagaaaa ttgcgagtgg tgagtttcat gttcctatcc
     1261 ttgatttaat ttttttgaag aacggtgaat gcccaacgca accagcgcgc caagcagacc
     1321 tccgatcatt ccaaagagaa agtgactggc aaagggatac gccacaccat agcggaagga
     1381 gccaaagggc cagccggtga tgagattcat agtgagttga cccagcggtt ttgtagcgtg
     1441 agccagaccg cccaacagaa ccaggaacca aatgtgtttg gtgaagcgcg gtgcataata
     1501 aaaaccaaag tccatgacga gaccggggag caggtagaac aaccagccga aaggattatc
     1561 gccttgcagc cccggcatga atgtcgatac cgatgcgccg atgctggtaa gcatgcctgc
     1621 accgcgaaac ttggatgatg cacgacccag gatcatgatc gccatccatt cgagcccatg
     1681 atgacctgga agcccaagcg acaggtctgt agtgcgatgc aagaccaccg ccatcacacc
     1741 gccaccgatc agcagtagcg cttcgaccca tgaaagtgac caatcagttt gagagacgct
     1801 tgatgtggtc tttagagatg atgttccatt catgattttt ctcctcaaca taaagagttg
     1861 ccttgccgtt ctttaaaatg ggacgaatcc attcgcccac atccacaacc gaatcatggg
     1921 caagctcaac ttgctccagc gtacacacac gatcaatatg tccgagcgtg cagagctcat
     1981 gcacggggta tttccctttc aactcgcgca agcccggcaa ccagagataa cgaccatcat
     2041 ctccagcgcg atgtagacca gtggcagcca gcgctccgat gatgccgcca tgtgtgcccg
     2101 tcaacccttc acatcgtatc ttggacattg aagctatctc acgcgcttca ggcatggtca
     2161 acactgtgac ctttgctttg cgaccccagt ccaaaacatc ttcgctgatc gagttccact
     2221 cggcgaggca caagccagta tctgacccga ctgcactttc acgaagcaag aattcgcgag
     2281 ccgcttccca aacgttatct gtgttttctg tttcgatcga aagacaagcc gaactgttat
     2341 gtgatgtata gggaatacgc gagtccacca gtaattgatg acgggtaata ccaagtgggt
     2401 gggcgagtcc gttttcagcg agccaatcgg caagctggcg gacgcgatgt cccgttccgc
     2461 ggctttcgag gttgtccgta tcgtcaattc cgagcaggat gtgcatgatg ctcctaaaat
     2521 tgcgtgtaat caattaagga aaagcttaat cgaaatttga aaataaaaac actttttcgt
     2581 atttaacgtt acttaacgac gatctccgcc acgtctttcg tccattccgc tttgggaatg
     2641 taagtgctgg aaagtttcat cgtgccatgg ttattgaaat cgaggatggt gttttcatcc
     2701 acctgttcga aggtcaatgt aaccgggttc gagctgcccg aaagcgagac ttcggtgaac
     2761 tcggtcaccc ctgccagagc gagcacgtct ttcaagtacg gaccttcttc ggtcttgcct
     2821 tctgcttgca cttgcgcgag tggcagggct ttcacagcat tgagggtcac gtcataggta
     2881 gaaccgtctg cccggataat ggtgaacaaa acttgagatg tatccgctga ggtttcgggt
     2941 gccgccgatc tttctgctgg cgcagcgcag gcagaaagta tcatgatgcc ggttgccgca
     3001 aaagataaaa gtagaaaaat acgtttcata ggctcctttt cgtagccagg tttttatgat
     3061 gcggtgacga tctcgccgct gtgacttgtg ttgtaacccg tcagcaatgc cagcatctca
     3121 cggaaattta ctgtttgaat gtattcaagc aatgggttga gattttcttc ctgttcacgc
     3181 ggcaagacga gatcatagcg ttcctcaaaa agcggaatga agtccaatcc gttttgatat
     3241 gcggcggctt gcagcccaag cgatacatcc accttgtttt ggatgaccaa attcgccgct
     3301 tcactatgtg ttttgacctg ttcctcgtac cccgaaattt tctccactgg aattttctgc
     3361 ttgcgcaatt cagcgtccaa ccataagcgt gtgccagaac cggggttgcg attcacaaat
     3421 cgtacgctgg gtctggcaat gtccgcgatc ttcttgatcc ctttgggatt tccgctggca
     3481 atgatcagcc cttgtgtgcg gtatgcgagt gtaacgatct ctacattacg atctggaaag
     3541 aggcgcttga cgaagggcgt gttgtactca ccgctttcat ccagcaggtg cgagccggag
     3601 atatggcaca gtccctgccg caagttgacc aacccatcca gcgaaccgac aggcaggctg
     3661 agcaggttga catactttgc cagctgttcg ctcacattct cgagcgcaag atcatggctg
     3721 cccgaaaaaa taacagctgg cttcttgctc ttgggcagga taatctcttg taatagaagt
     3781 gcatctgcct tggcgcaata aaacttttcc attaccttgc cggtcttacg aatctcgctg
     3841 acttcgatca gcccggcaga ctcaagcgcc aggatgtggt gccgcaccca ggcgggggat
     3901 tgtttcatcg tccgtgcgag tagcgtgagt gtggcaggag ccgccatcag caggcgtaat
     3961 atatccaacc tgcgcgggtc tgatagggtt tttatttggt caaacgcctg gagttggaca
     4021 atttttttca ctcgattaaa taaaacctta atctatttta acataatcat tgcctggggg
     4081 ctaaacagca ttcaactgga ttttttgcca caaagaatat aaatgttaca acgaaaaacc
     4141 accgagacaa agtccctttt tcctgtttct tgctgaagat tcacatccac atctgtagca
     4201 tacaataata tgctggcttg aaagagcaaa attgttgcag atgcgaattc aagcgttttt
     4261 gacattaaat atgcacttcc cgaactaggt gaccgggaag tgcggcataa tcgtttggtg
     4321 tggtatgtat taaatgtagt tcaacgtggg tcgaaattta
//
LOCUS       DAOQFT010000159         4355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10820_length_4355_cov_6.153023, whole genome shotgun sequence.
ACCESSION   DAOQFT010000159 DAOQFT010000000
VERSION     DAOQFT010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10820_length_4355_cov_6.153023"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PLR93_05370"
     CDS             complement(<1..1339)
                     /locus_tag="PLR93_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH26427.1"
                     /translation="MSEPLLKVESLSKNFDRLPVLRDVSFQVDKGEVIGLVGRQGAGK
                     STLLHLLGGAIPASSGTINYLGKSRRFFNRLEAQSLGIETVYQTSSLINYFDFSSQIF
                     LDRESDIKNTRQTSGLVEQFNVTQNMLLGREIMKVPSLGVLDHDKMVDIAQELLAEFN
                     LPKYLAVEKVRNLTDEQRQIVLLLRMLYHPCQLLLLDDILPILSFDRQNILLERIRKL
                     ADQGTSVIISSDDLKHLFTVTDRIIVLYEGRLVADRRTSESTPREIVELMVGSAAHGQ
                     VSPLIWALESYHTAQQQTEDLRRTQASLKQSLDARTSLNRQLVERLSNQLVALDQLNA
                     ALQATQRRLITEREDERKALARELHDQIIQDMLGLIYHLEEIEEKAGSAGVKQDVGLV
                     RSGIRSLVEELRQVCSDLRPPTIDHHGLPAAINSFANEWADRSDVEIHLEIDPA"
     gene            1405..2454
                     /locus_tag="PLR93_05375"
     CDS             1405..2454
                     /locus_tag="PLR93_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26428.1"
                     /translation="MSLVRVLLADDHTVVRAGLRNALEVLPNLEIVGEVGDGIELMNA
                     ISQSPIDLLVMDASMPDFEPISAVQQIKEKYPSIKILVVSAYNDESYVVGLLKAGASG
                     YHLKDQPLSDLQLAAQRVLSGQRWISSNLIDRLLTRQAPTPSTNLPYLTRRQRELLRL
                     ISQGADNRNIAQALDLSIKTVENHLTALYRVLGVDSRLKALNYILRHPELLASTGQEM
                     ADSNPSVKGQEFTFLIVDDNPRYRQQLGRLIGKIYPSIKLYEADTTAEALSISKQVQP
                     QLAFIDVVLDDEDGIQCARKLRTVSPQTRIVVISAYPDKEFRRQALSAGVDVFLDKKD
                     LDTASVQQVLEDALR"
     gene            complement(2459..3688)
                     /locus_tag="PLR93_05380"
     CDS             complement(2459..3688)
                     /locus_tag="PLR93_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNH26429.1"
                     /translation="MKITADQAFLRETNLSLVVRLIHNQSPLSRAQLAGITGLNKSTV
                     SSLVDELIERGLVYETGSNTGGTGRPATLLEINPNAGHIIGVELGVDFVSVAVTDILG
                     NIVWRRREDANPADDQEKMINQTLQIVKDAMSIGKRKNYKFLGLGLSTPGTVDIQKGL
                     LIFAPNLHWHNVPFVQIFSEQTKLRVFVENDANAAAIGEHLFGTARHTNDFLFVFAGV
                     GIGGGLFLDGKLYRGKNGYAGEIGHSPIMAEPSQTVCHCGNRGCWETYANQYSIIQRV
                     QARLEVKRSSIIPKLMAEQNAPLGIPLIKQAADAGDREAIDSFYEAGHAMGQGLAGLI
                     NIFNPEKIVIGGPLSIAGEYMLPAIKQTVSQHAMPGIKPQAEIGLSPFGPDASLIGAV
                     AIVVDSILSSVPHIQKA"
     gene            complement(3890..>4355)
                     /locus_tag="PLR93_05385"
     CDS             complement(3890..>4355)
                     /locus_tag="PLR93_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNH26430.1"
                     /translation="AKWVKDYPIVSIEDGLAQDDWDAWKSLVKTIGDKCQIVGDDLLV
                     TNPERVRRAIKENAANALLVKVNQIGSLTETIEAVDLCQRAGWRAISSHRSGETEDAT
                     IADLAVALNAGQIKTGAPARSDRVAKYNQLLRIESELGDTAKYAGWDALKVK"
BASE COUNT         1104 a         1032 c         1005 g         1214 t
ORIGIN      
        1 gagcagggtc gatctctaaa tggatttcga catcactgcg atctgcccat tcattagcga
       61 atgagttaat cgcagcagga agcccatgat gatcaatggt gggcgggcgg aggtcactgc
      121 atacctgtct gagttcttcc accagacttc gaattccgct tcttaccaag ccgacatctt
      181 gtttcacccc tgcagagcct gccttctcct cgatctcttc caaatgatag atcaaaccca
      241 gcatgtcttg aatgatctgg tcatgcaatt cgcgggctag agctttgcgt tcatcttcgc
      301 gttcggtgat gagacgcctt tgcgtggctt gtaaggctgc gttcaactgg tcaagcgcta
      361 cgagctgatt gctcaaccgc tctacaagtt ggcggtttaa cgatgtgcgt gcatctaggc
      421 tttgctttag agaagcttgt gttcggcgta aatcttctgt ttgctgttgg gcagtgtgat
      481 aactttccaa tgcccaaatt aacggagaga cctgaccgtg agcggctgag cccaccatca
      541 actccacgat ctcacgcggc gtggactcgg atgtgcgtcg atctgcaacg aggcgacctt
      601 cgtatagcac aataattcgg tcagtgactg taaatagatg ctttaggtca tcactgctaa
      661 tgatgactga agtcccttga tctgcaagtt ttcgaatgcg ttctaataaa atgttctggc
      721 ggtcaaaact taatatgggc aaaatatcat ccagcagtaa tagctgacat ggatgataaa
      781 gcatgcgtaa cagaagaaca atctgacgtt gttcgtctgt cagattgcgt actttttcca
      841 cggcaagata tttgggtaaa ttgaactctg ctaaaagttc ttgggcaata tccaccattt
      901 tgtcgtgatc caaaacccca agcgaaggga ccttcatgat ctcacgccct agaagcatat
      961 tttgggtcac gttgaattgt tccaccaaac ctgatgtctg acgggtattt ttgatgtcgg
     1021 attctcgatc taaaaagatt tgcgaggaga aatcaaaata gttgatcaag cttgatgttt
     1081 gatagaccgt ttcaattccg aggctttgcg cttccagccg attgaagaac cgcctactct
     1141 tgccaagata gttaatcgtc ccagatgagg cgggaatggc tccaccaagt aaatggagta
     1201 aggtcgactt ccccgctcct tgacgtccca ccagcccgat gacttcacct ttatccactt
     1261 gaaacgaaac atcccgcaac acgggcaaac ggtcgaagtt tttggaaaga ctttctactt
     1321 tcaacaaggg ttcagacata atttatttta acctgatttt gtgggcttct tttcagtttt
     1381 tgttacttac ggcataatag atgcatgtct ctcgtacgag ttcttttagc tgatgatcat
     1441 accgttgttc gggctggttt gcgaaatgcc ctggaagttc ttcctaatct tgaaattgtg
     1501 ggtgaagttg gcgatggcat tgaattgatg aatgccattt cacagagccc tatcgattta
     1561 ttggtaatgg atgccagcat gccagatttt gaaccgatct ctgcggttca gcagatcaag
     1621 gaaaaatatc cgtcgatcaa aattcttgtg gttagtgctt ataacgatga atcttatgta
     1681 gtggggcttt tgaaagcagg cgcaagtggt taccacttaa aggatcagcc attatcagat
     1741 ttgcagttgg cagcgcaacg cgtattaagt ggacagcgtt ggatatcgag caatttgatt
     1801 gaccgtttgt tgacgcgtca ggcgccgacg ccttctacca atttaccgta tctcacccgc
     1861 cgccaacgtg aattgttgcg gctgatttct caaggtgccg ataatcgcaa tattgcccaa
     1921 gctttagact taagcatcaa gacggttgaa aatcatctta cggctttgta tcgcgtctta
     1981 ggggtggata gccgcttaaa ggcattgaac tatatcttgc gccatcctga gttgttggct
     2041 tctaccggtc aagaaatggc agattcgaat ccttccgtaa aaggacaaga atttacattt
     2101 ctaatcgtgg atgataaccc tcgttatcgt caacagcttg gtcgtttgat tgggaagatt
     2161 tacccatcga tcaaattgta tgaagccgat accactgccg aagccttgtc tattagcaaa
     2221 caagtccaac cgcaattggc atttattgat gtcgttttag atgatgaaga tggaattcaa
     2281 tgtgcccgga agttgagaac cgtttcgcct caaactcgca ttgtggtgat cagcgcctac
     2341 ccagacaaag agtttcgccg acaagcttta tctgccggag tggatgtatt cctcgataaa
     2401 aaagaccttg atactgcttc agttcaacaa gttcttgaag acgccttgcg gtagcctttt
     2461 atgctttttg aatatgtgga acgcttgaaa gaatactgtc cacaacaatt gcgacggcac
     2521 cgatcaagct ggcatctgga ccgaatggag agagtccgat ctctgcttgg ggtttgatgc
     2581 ccggcatggc atgttgactc accgtctgtt tgatggctgg cagcatatat tcccctgcaa
     2641 tgcttagcgg acctccaatg acgatcttct cggggttgaa aatattaatc agcccagcca
     2701 atccctgccc catggcatga ccagcctcat agaacgagtc gatagcttct cggtcgcctg
     2761 catccgctgc ttgtttgatg agcggaatac ctaatggcgc attctgctct gccatcaatt
     2821 tggggatgat gctggagcgt ttaacttcca aacgggcttg cacccgctga ataatggaat
     2881 attgattggc ataggtttcc caacaaccac ggttgccgca atgacaaaca gtttgtgaag
     2941 gctccgccat gataggggag tgaccaatct cacccgcata cccatttttg ccgcgataca
     3001 gtttcccatc taagaatagc ccgccaccaa ttccaacccc tgcaaataca aaaagaaagt
     3061 cattagtatg acgtgctgtg ccaaaaagat gttcgccaat ggcggctgca ttggcgtcgt
     3121 tctcaacaaa aacccttagc ttagtttgct cagaaaaaat ttgcacaaac ggcacattgt
     3181 gccaatgaag atttggcgca aaaatcaaaa gacctttttg aatatctacc gtgcctggtg
     3241 tggaaagtcc cagccctaaa aacttatagt ttttgcgttt accaatggac attgcgtctt
     3301 ttacgatctg caatgtttga ttgatcattt tttcctggtc atctgctggg tttgcatctt
     3361 cacgcctgcg ccaaacgata ttgccaagaa tatctgtcac agcaacagac acaaagtcca
     3421 caccgagttc tacaccaatg atatgacccg cgtttgggtt gatctctaac aaggtagctg
     3481 ggcgtcccgt cccacctgta ttgctgcccg tttcatacac taagccgcgt tcaattaact
     3541 cgtcgaccag gcttgaaacg gtcgatttgt tcagcccggt aataccagca agttgtgcgc
     3601 gcgataatgg agattgatta tgaattaacc ttacgaccaa tgaaagattg gtttctcgta
     3661 aaaatgcttg atcggcagtg attttcatag gctccctagg attagtatgt tggggcaacc
     3721 aacaaattat agcgctttga aggggattgt caaattaaac atcgacaggc gcaaggtcgc
     3781 ttctttctgg accttgtgcc tgtttacccc tttctagctt tttccttgcc ttgagtttat
     3841 gcaactataa aaaaactccg ttgagacaaa ctcaacggag tttttcagct tactttactt
     3901 tcaaagcatc ccagcctgcg tacttggcag tgtcacccag ctctgattcg atgcgcagaa
     3961 gttgattgta cttcgccaca cggtcagaac gggcaggggc gccggtcttg atttgcccag
     4021 cattgagtgc aactgcgagg tcggcaatgg tcgcatcttc ggtctcgcca gagcggtgag
     4081 atgaaatggc acgccagccc gcacgctgac agaggtccac agcttcaatg gtctcagtga
     4141 gagaaccgat ctggttcact tttaccaaga gtgcattcgc agcattttcc ttaatagcgc
     4201 ggcggacacg ttcagggttc gtgaccaaca aatcatcacc cacgatttgg cacttatcgc
     4261 caatggtttt gacgagagat ttccaggcat cccagtcatc ttgagcaaga ccatcttcga
     4321 tggaaacgat ggggtaatct ttaacccact tcgcc
//
LOCUS       DAOQFT010000160         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10858_length_4345_cov_6.603263, whole genome shotgun sequence.
ACCESSION   DAOQFT010000160 DAOQFT010000000
VERSION     DAOQFT010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_4345_cov_6.603263"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLR93_05390"
     CDS             complement(<1..832)
                     /locus_tag="PLR93_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH26431.1"
                     /translation="MKTKHWIAFIALGGIWSSSFLWIKIGVQEASPNVLVAFRMLFGA
                     LMSTAIALYQRSEWPTDRKTLINFAILGPTSLAIPIFFISWGEQVIDSAVASILNATV
                     PLFTLILAHFWLQDDKITPQKLIGLLVGFAGILILFSKDLLASQHQNVIGQLAVIVAA
                     LFYAWSAVFGRKTTAHIKGMSRAALPVITATIFMWLTIPFTEKPFVLPQLPITWISFL
                     WLGLLGSGITSLLFYYLLHEIGPTRTTLVTYIFPVGGVLLGVIFLKETLTWQLIAGTI
                     L"
     gene            complement(944..1819)
                     /locus_tag="PLR93_05395"
     CDS             complement(944..1819)
                     /locus_tag="PLR93_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNH26432.1"
                     /translation="MKTKHWFIFITLGLIWSSSFLWVELALREVGPITLVAFRAFFGF
                     LFGVAVVVTQRIPLPRTLKEWIPLIVLGLTNIAIPFFLISWGQKSIDSGVASILDATV
                     PLFTILVAHFMLHDDKMTTPKVLGLLTGFAGVVVLMSKDLGASTSSLLGQLAVILACI
                     FYAASSIIARVFTEGTSATMRGLGPLVSASAVMITSAFIFESPIKIPTLPITWIALLW
                     MGILGSGLAFIMLFYLIHEIGPTRTTMVTYLFPLGGVTLGVIFLKEALTWQIVAGAVL
                     IILSLVIANWQTKQA"
     assembly_gap    1879..1978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1986..2405)
                     /locus_tag="PLR93_05400"
     CDS             complement(1986..2405)
                     /locus_tag="PLR93_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH26433.1"
                     /translation="MVTLKPKARVTATPAADPAIANAFFSAKLTYETDPSDVYADMKN
                     ELADFILIDVRSTADYAKSHAQGAISLPQVNINTETTSKLPKEKLLVVYCWGPGCNGA
                     TKAAAKLSALGFHVKEMIGGIEYWEDKERYPVERGWL"
     gene            2518..3414
                     /locus_tag="PLR93_05405"
     CDS             2518..3414
                     /locus_tag="PLR93_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNH26434.1"
                     /translation="MYTAGMELHHLKTFVIVAEERNLTRAADRLFMTPPSVSAHIKAL
                     EDELGVTLFVRKPQGMEITEHGEILRVKAEKILNSTREMVNLSKKLQASLTGTTRIGL
                     SATSVYQRAAKLISQMQKEHPDIELTFNASCTHKIIQGLKNHSMDAGYMFGAIEDETI
                     TGYELDVADLVITAPAAWKDQVAEAGWKEIAALPWIGSSYYCPFQTAAEKLFKKRKLA
                     MNHVVWMDDEFNKCALVEAGVGLALLERSEAEQAALGGKIFIWKTEPIKCKLSWVYLR
                     NRGDDPLIAALTAEVLNIWGKA"
     gene            3411..3863
                     /locus_tag="PLR93_05410"
     CDS             3411..3863
                     /locus_tag="PLR93_05410"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26435.1"
                     /translation="MISYSIRKATPEDLPVLVELCAEHAEYEQAEYSPLGKADRLREH
                     FFGTPPSAFCLVAEVDQTLAGYATYSKEFSTWDAGFYIHMDCLYLRPSFRGYGIGKEL
                     ITQIREEARGLDCNLIQWQTPKFNTRAIKFYERIGAKQKEKVRFYLNV"
     gene            3967..>4345
                     /locus_tag="PLR93_05415"
     CDS             3967..>4345
                     /locus_tag="PLR93_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26436.1"
                     /translation="MSQPNLPDFDSLWDYSNPDQTETKFREVLPQFPDGNERLELLTQ
                     IARTQGLQRKFDEAHKTLDEVEGQLSVDSSRARVRYLLERGRVFNSSKHPDEARPFFE
                     QALTMAQELKEDFYAVDAAHMLAI"
BASE COUNT         1133 a          993 c         1132 g          987 t
ORIGIN      
        1 tcagaattgt acccgcaatt aattgccagg ttagggtttc tttgaggaag atcacgccaa
       61 gcagaacgcc gccgactggg aagatatacg tgaccaaagt ggtgcgagtg ggaccgatct
      121 catgcaggag atagtaaaag agcaatgaag tgatgcctga gcccagtaag cccagccaaa
      181 ggaatgagat ccatgtaatg gggagttgcg gcagaacgaa aggcttttca gtgaagggga
      241 tcgtcaacca cataaagatc gtggcggtaa taactggcaa cgccgcacga gacatgcctt
      301 tgatatgggc agtggtcttt cgaccgaaca cagcgctcca ggcatagaac aatgccgcaa
      361 caatgacagc caattgaccg atcacgtttt gatgctgact ggcgagcagg tctttgctga
      421 acaggatgag aatgcctgca aagcctacca gcaagccgat cagtttctgc ggcgtgatct
      481 tatcgtcttg aagccagaag tgtgccagaa tcagcgtgaa caagggcact gttgcattta
      541 atatcgaagc gacagccgag tcgatgactt gctcgcccca tgaaatgaag aagatgggaa
      601 ttgccaaact ggtgggtccg aggatggcaa aattgatgag cgtcttgcgg tctgtgggcc
      661 actctgatcg ttgataaagc gcaatggcag tggacatcaa cgcgccgaat aacatgcgga
      721 atgccaccaa cacattgggg ctggcttctt gaacgccgat cttgatccaa aggaaggaag
      781 aactccatat gccgccaagg gcaatgaagg cgatccaatg ttttgttttc aaactatcct
      841 gctttctgaa attgagggtg cgggcttcac aaaaccggcg aaatgatcag gaacaatgat
      901 tggcaattcg aggttgaaac cagatgcatc tgtatatgtg ttcttatgcc tgtttggttt
      961 gccagtttgc aatgacaagg ctgagaatga ttaacaccgc tccagccacg atctgccagg
     1021 tcagcgcttc tttgaggaag atcacgccga gagtgactcc gcccagcgga aatagatagg
     1081 tgaccatggt ggtgcgggtg ggaccaattt catgaatgag atagaacaac ataatgaacg
     1141 ctagtcccga accgaggata cccatccata acaaggcgat ccaggtaatg ggcagggtgg
     1201 gaatcttgat tggcgattcg aagataaaag cggaggtaat catgacggct gatgcagata
     1261 caagcggacc taatccacgc atagtggcgg atgtgccttc agtaaacaca cgtgcaatga
     1321 tggagcttgc tgcataaaag atacaagcca ggatgacagc caattgccca agcaaagaac
     1381 tggtggaggc gccgaggtct ttgctcatca gaacgaccac accagcgaaa ccggtcaata
     1441 agcccaacac tttgggcgtt gtcatcttgt cgtcgtgcag catgaagtgt gccacgagaa
     1501 tggtaaagag tggcacggtc gcatcgagaa tggatgccac acccgagtca atggattttt
     1561 gcccccagga aatgaggaag aagggaatag caatattggt cagcccaagc acgatcagcg
     1621 gaatccattc ttttagtgtg cgcggtaaag ggatgcgctg agtcactaca actgccactc
     1681 caaacaagaa accaaaaaaa gcgcggaagg caacgagtgt aatgggaccg acctcacgca
     1741 gagcaagttc gacccataaa aaggatgaac tccagatcaa gccgagggtg ataaagatga
     1801 accagtgttt tgttttcaat ttaactccta aatgattaat gtatgcagcg attgtacgcg
     1861 gtcagcttta ccttttacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     1981 ctcctttaga gccagccgcg ttccacgggg tagcgttctt tatcttccca gtattcgatg
     2041 ccgccgatca tctctttgac gtgaaagccg agagcactga gcttggcggc ggcttttgtc
     2101 gcaccgttac agccgggacc ccaacaatag accaccaaca gcttttcttt aggcagcttg
     2161 gacgttgtct ctgtattgat gtttacctgc ggcaaactga ttgcaccctg cgcatggctt
     2221 ttggcgtagt ctgctgtgct acgaacatcg atcaggataa agtctgccag ttcgttcttc
     2281 atgtccgcgt acacgtcgga tgggtcagtc tcgtacgtca gcttggcgga aaagaaagcg
     2341 ttggcaatgg cggggtctgc tgccggggtg gctgtgaccc gtgctttggg tttgagggta
     2401 accatattgt ctccttgagt tggaatgctt caagtgtatg gaaaagaagc ccttttgtga
     2461 atttgaggct ttcgaacata gcgttcggtg aagcaaaacg ctcggcagaa gggaatcatg
     2521 tatactgcgg gcatggaact ccatcactta aagacctttg tcattgtggc tgaagaaaga
     2581 aacctgaccc gtgctgccga ccgtttgttt atgaccccgc cttcggtcag cgcgcacatc
     2641 aaggcgttgg aagatgaatt gggtgtcacc ctcttcgtcc gcaagccgca gggcatggaa
     2701 attaccgagc atggcgaaat cctgcgcgtc aaagccgaga agatcttgaa ctccacgcga
     2761 gagatggtca acttatccaa aaaattgcag gcatctctca caggcacgac ccgcattggg
     2821 ttaagtgcca cgtcggttta tcaacgtgcg gcgaagctga tcagccagat gcagaaggaa
     2881 cacccggata tcgagttgac ctttaacgca tcttgcacgc acaagatcat tcagggattg
     2941 aagaatcact cgatggacgc cgggtacatg ttcggtgcca ttgaagacga aacgatcaca
     3001 ggctatgagt tggacgttgc cgaccttgtc atcacggctc cagcggcgtg gaaggatcaa
     3061 gtcgctgagg caggctggaa ggagatcgcg gcgctgccct ggatcggttc atcgtattat
     3121 tgtccctttc agacggctgc cgagaaactc ttcaagaagc gcaagctcgc catgaatcat
     3181 gtggtctgga tggatgatga attcaacaag tgtgccttgg tcgaggcggg cgttgggctg
     3241 gcattgctcg aaagaagcga agcggagcaa gcagcactgg gcgggaaaat ttttatctgg
     3301 aagaccgagc cgatcaaatg caagctctcg tgggtctatt tgcgcaaccg gggggatgat
     3361 cctctcatcg ctgccttgac cgccgaggtt ctgaatattt ggggcaaagc atgatctcgt
     3421 attcgatccg aaaagcaacg ccagaagatc tgcctgttct tgttgagttg tgcgccgaac
     3481 acgctgagta tgaacaagcg gaatattctc cgctcggaaa agcagaccgt ttgcgcgaac
     3541 acttttttgg aacacctccc tctgccttct gtcttgttgc agaagtggat caaactctgg
     3601 caggctatgc aacttattcc aaagaatttt ccacatggga cgcaggcttt tacatccata
     3661 tggattgtct ttacctacgc ccgtcttttc gcgggtatgg aattggaaaa gaactgatca
     3721 ctcagattcg agaagaagcg cgcgggcttg actgtaatct catccaatgg caaacgccga
     3781 aattcaatac acgtgccata aaattttacg aacgcatcgg ggcaaagcag aaagaaaaag
     3841 ttcgattcta tctaaacgtt tagagataac ccttctccgg taggttgctt gcccatgggg
     3901 cttggactac catccattta cgcttaaaat ccaaagatta tctttatcgt attatggaga
     3961 tgtaccatgt cacaaccgaa cttacctgat tttgattcgc tttgggatta ctcgaacccc
     4021 gatcaaaccg aaaccaagtt tcgcgaagtt ttgccacaat tccccgatgg caatgaacgt
     4081 ttagagttat tgactcagat cgcgcgtacg caaggcttgc aaagaaagtt tgacgaagca
     4141 cataaaactt tggatgaagt agaaggtcaa ctctctgtcg attcttcgcg cgccagagtt
     4201 cgttatcttt tggaacgcgg gcgcgttttc aattcatcca aacatcccga tgaagcccgt
     4261 ccgttctttg agcaggcgct gaccatggca caggaattga aagaggattt ctacgccgtg
     4321 gatgcagctc acatgctggc gatcg
//
LOCUS       DAOQFT010000161         4336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_10888_length_4336_cov_6.595422, whole genome shotgun sequence.
ACCESSION   DAOQFT010000161 DAOQFT010000000
VERSION     DAOQFT010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10888_length_4336_cov_6.595422"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3874)
                     /locus_tag="PLR93_05420"
     CDS             complement(<1..3874)
                     /locus_tag="PLR93_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26437.1"
                     /translation="MNFNSSNGRFSEKSIGRVLMGIVFLYQAIAVVVVVWALVVAAQW
                     VREPFLGSFYEHTLVFTDPAADSTGVWAFNKIVNSGDQLIAINGTDVKNSRDVREVLS
                     SFVPGENVTATVKFKNGETRDLSVELQRFPEDAVSIYLILPSIISFVFLVLSIWTFGL
                     RRNEPSGRAFALFASSFAIVTGTIFNLWTSHEFTTIWTAAVAIAGGANIALALSFPAS
                     PRLIINRPYLRWMGFVLSFVLTAITIPYLFNSQNPTEYIAKWQNLYYFAAVGILSLLG
                     SNLYYSLYSQSPVVKLQARTTLIGAVFAFLPLTVYLVFGNIFSIKFSPWLFLPVVIFP
                     AIMGYNIMRFRFLRTDDLARRGVMYVLLTAIVTMGYALVAAGAAFLFGNSLPQNYLAG
                     FFILVLAIVLEPLRIRLQNLTDVLFFRGSRVVAEQLEDFSHKLTTALDLNAISSLLRD
                     QIASTLAPDQIHIFTYDSLNDFFVASPGDGRRTTTDIRFTSASPLVSYFRSERLPLYL
                     DNGTDLPDVLKPEQSRIALLGARLFIAFPGKDKPNGWLALGPRLTNQAYSPSDLRFLE
                     NISDQASVAIERVQTVAHLERRIQEMNALTRVSQGVNVTLTFDDVLELIYAQTAQIVP
                     TSHFHITLYDKDSDYYYYGFCLENNERMHERENQPFSSNVGLSPDVIRKSRPIITQDY
                     IRECQARNHTPTIENIFAWIGVPLNAGAESIGALSVGSRDASTIYTRAQVDLLQAIAD
                     QTAGAIVKARLLKETQERAHQLTTLNEITRQLTSTLELNPLLQNILENAVGILNCEAG
                     SLLLVDDQTDELVFRVTVGPVAGNLIGKRLARGTGIVGRAVETRKPVIENEEQTSNRF
                     NGVDQQTGFVSRSLLAVPLQAKDRVIGVVEVINRRDGLPFVQNDQTLLTAFAGQAAVA
                     IENARLYTLTDQELANRVEELSVMQRIDRELNASLEMDRAMRITLDWALRQSGADAGL
                     IGTLEETKLRVMAHQGLDEQMASLPDQVMKIELPSMLAAVETGSPQNVEVASGASKNK
                     LLMASQTQMVIPIRRETMVIGLLYLESSSGSQVDIDFLTRLTDHAAIAISNAQLYSEV
                     QRANLAKSDFVSFVAHELKNPMTSIKGYTELLASGAVGQITEMQTNFLGTIKSNVERM
                     STLVSDLNDNSKIEAGRLRLEFKATQASDLVDEVIRTFKRQLEDKKQTLELSIPPNLP
                     AMWADRIRVGQVLTNLVSNAFKYTPESGAIQVGVEESPNHWDPDGAPRVIHLWVKDSG
                     IGMTPEDQQKIFQKFFRSDDPKAREAPGT"
     gene            complement(4056..>4336)
                     /locus_tag="PLR93_05425"
     CDS             complement(4056..>4336)
                     /locus_tag="PLR93_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26438.1"
                     /translation="FFPGKTIVLKRANVLYGDFVTIIEELRSSDYLVLYPIVREKQPE
                     TEKILGELEGVVEPEKVIYINGLEYIQIYKVSDIPEAVYEKLLAKNEP"
BASE COUNT         1204 a         1083 c         1005 g         1044 t
ORIGIN      
        1 cagtccctgg cgcttcgcga gccttggggt catcggaacg gaagaatttc tggaaaatct
       61 tttgctgatc ttcgggtgtc ataccaatac cgctatcttt tacccacaag tggataacac
      121 gcggggcgcc atcggggtcc caatgattcg ggctttcttc cacgccgact tgaatagcgc
      181 cgctttctgg ggtgtacttg aaggcattac tcacaagatt ggtcaacacc tgccccacac
      241 gaatgcggtc tgcccacata gcgggtaagt ttggtgggat gcttaattca agggtttgct
      301 tcttatcttc aagttgacgt ttaaaggtgc gaatgacttc atccaccaaa tcagaagctt
      361 gtgtggcttt gaattccaag cgtaggcgac ccgcttcgat tttggaatta tcgttgaggt
      421 ccgataccaa tgtggacata cgctccacat tcgatttgat cgtacccaag aagttggttt
      481 gcatttcggt gatctgtccc accgccccgc tggctaataa ctcggtatag cctttgatcg
      541 aggtcattgg gttcttaagc tcatgcgcca cgaaagagac gaagtcgctc ttggcaaggt
      601 tggcgcgttg cacttcgcta tacagctgag cattcgaaat cgcgatagcg gcatggtcgg
      661 taaggcgggt caaaaagtcg atatccactt gcgagccgct ggaactctcc aaatatagca
      721 acccaatcac catcgtttca cgacggatgg gaatcaccat ttgagtctgt gatgccatca
      781 gtagtttatt ttttgaagcc ccgctggcta cttccacatt ttgcggtgaa cctgtttcaa
      841 cagccgccaa catggacggc aattcgatct tcatcacctg gtcaggtagt gaagccattt
      901 gttcgtccaa gccttgatgt gccatcacac gcaattttgt ttcttcgagc gtaccgatca
      961 aaccagcatc tgcaccagat tgtcgcaacg cccaatcgag tgtgatgcgc atggcacgat
     1021 ccatttcaag gctggcattc aattcacggt caatgcgttg cataaccgaa agttcttcta
     1081 cgcgattggc aagttcttga tctgtaaggg tatacaaacg cgcattttcg atagcaaccg
     1141 cagcctgacc cgcaaacgca gttaacaagg tttggtcatt ctgtacaaag ggcagaccat
     1201 cgcggcggtt gatgacttcc accacgccaa ttacgcggtc tttcgcttgc agtggaacgg
     1261 cgagtaacga gcggctcaca aaccctgttt gttgatccac tccattgaag cggttcgacg
     1321 tctgctcttc attttcaatc acaggcttgc gcgtttcaac cgcccgcccc acaatgcccg
     1381 ttccgcgagc aagacgcttg ccgataaggt tgccagctac aggtcctacc gttacgcgga
     1441 aaactagttc atctgtttga tcatcgacca gcaacaaact ccctgcctcg cagttcaaaa
     1501 tgcctacagc gttttccaaa atgttctgca aaagcggatt caattcaagc gtcgaagtca
     1561 actgacgcgt aatctcattc aaggttgtca attgatgcgc acgttcctga gtctctttca
     1621 gcaaacgcgc tttaacgatt gcgccagcgg tttggtctgc aatcgcctgc aataggtcta
     1681 cttgagcgcg agtatagatc gtggaggcat cacggctacc aacactaagg gcaccaatcg
     1741 attcagctcc agcgttaagc ggcacaccga tccatgcaaa gatattttcg atggtgggag
     1801 tgtgattccg cgcctgacat tcacgaatgt aatcttgagt aatgatcggg cggctcttgc
     1861 ggataacatc tggagaaagc ccaacattgg acgagaaggg ttggttttcg cgttcgtgca
     1921 tacgctcatt attttcaaga cagaaaccat aatagtaata atcactgtct ttatcatata
     1981 gcgtgatatg gaagtgagaa gttggaacga tctgtgcggt ttgagcatag attaattcca
     2041 agacatcatc aaaggtcagc gtaacgttta cgccttgtga aacgcgggtc aacgcgttca
     2101 tttcttgaat tcggcgttcc aagtgcgcca ccgtctgcac gcgttcaata gcaacggatg
     2161 cttggtcgct gatattttca aggaagcgca aatcgcttgg agaatatgcc tgattcgtca
     2221 ggcgtggacc caaagccagc cagccattcg gcttatcctt accggggaat gcaatgaaca
     2281 aacgggcacc caacaacgca atacgcgact gttccggctt aagcacatca ggcaggtcgg
     2341 taccattgtc caaatacagc ggcaagcgtt cgctcctgaa gtaactcacc aatgggcttg
     2401 cagatgtaaa acgaatatca gtcgtagtac ggcgtccgtc gccgggagag gcgacgaaga
     2461 aatcattgag ggagtcgtat gtaaagatgt gaatctggtc tggggcgagg gttgaggcga
     2521 tctgatcccg caggagcgaa ctgatggcat tcaaatctag agcggtggta agtttatgag
     2581 agaagtcttc caactgttct gccaccaccc gcgaaccacg gaagaataga acatccgtca
     2641 ggttttggag gcggatgcga agcggttcca aaacgatcgc cagcaccaaa atgaaaaagc
     2701 ccgccagata attctgaggc agggagtttc caaataagaa cgcagcacca gcagctacta
     2761 gggcatagcc catggtgacg attgcagtta acaaaacata catcacgccc cgccgagcaa
     2821 gatcatcagt acgcaagaag cggaagcgca tgatgttgta gcccatgatc gcggggaaaa
     2881 tcacaaccgg cagaaacagc cacggagaaa acttgatgga gaaaatattg ccgaagacca
     2941 aatagacagt caacggcaaa aaggcaaaga cggcgccgat caatgtagtg cgtgcttgta
     3001 gcttaacaac aggcgattgt gaatacagtg aataatataa attggatcca aggagcgaca
     3061 aaataccaac agccgcaaag tagtaaagat tttgccactt tgcaatgtac tcggtggggt
     3121 tttgtgaatt gaaaagatat gggatcgtaa tcgccgtcaa aacaaaacta agaacaaaac
     3181 ccatccaacg gagataagga cgattgataa taagccttgg tgaagccgga aacgataaag
     3241 ccaacgcaat atttgcccca cccgcaatag caacagccgc cgtccatatg gttgtaaatt
     3301 cgtggctggt ccacaagttg aagatcgttc cggtgacaat tgcaaaagag gatgcaaata
     3361 aagcaaacgc acgaccagaa ggttcattgc gacgtaaacc aaaagtccaa atactaagga
     3421 caagaaaaac aaaactgata atcgacggga gaatgagata aatactaacc gcatcttcag
     3481 ggaagcgctg caactccaca ctcaggtcgc gagtctcgcc attcttaaat tttacagttg
     3541 cagttacatt ttcgccgggc acaaaacttg aaagtacttc acgcacatca cggctgttct
     3601 taacgtccgt cccattgatg gcaatcaact gatcgccgct atttacaatt ttattgaatg
     3661 cccaaacccc ggtcgagtcg gctgctgggt ctgtaaaaac cagtgtatgt tcgtagaaag
     3721 aacccaagaa aggctcacgt acccactgtg ctgccaccac cagcgcccaa acaacgacaa
     3781 ccacagctat cgcctgatac aagaacacaa tccccatcag gacgcgccct atggactttt
     3841 cagaaaatcg accgttggat gagttgaaat tcatagctta atgactgaaa ttttactcca
     3901 caatcctgct caaatcatat ggtactttca tgacaaaggc gtaaggtggg ataggaaaac
     3961 cccgcctctt tctggacttt tgcctgtttt ccccctccaa aacttcccta cccgtagcct
     4021 aaggaaaaac ggcgcttagc gcgccgttta atgtcctacg gttcattttt tgccaataac
     4081 ttctcatata ccgcttcggg aatatcactc actttataga tttgaatgta ctccaacccg
     4141 ttaatataga taaccttttc aggctctaca accccttcta actcacctag aattttttct
     4201 gtctccggct gtttttcgcg aacaatcgga tataacacca aataatcaga gcttctcaac
     4261 tcttctataa tggtcacaaa gtcaccatat aaaacattgg cacgcttcaa aacgatcgtt
     4321 ttcccgggga agaaat
//
LOCUS       DAOQFT010000162         4272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11131_length_4272_cov_5.473322, whole genome shotgun sequence.
ACCESSION   DAOQFT010000162 DAOQFT010000000
VERSION     DAOQFT010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11131_length_4272_cov_5.473322"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..3396)
                     /locus_tag="PLR93_05430"
     CDS             complement(133..3396)
                     /locus_tag="PLR93_05430"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26439.1"
                     /translation="MSAATRSSKDWLQLASLGEQLRSEDSLSAQRDRIAAMANRLIEG
                     KVDVWLHENIFRLPDWDDGYIFPTQPALDGMKRAIKTGKLARKKKSSGKKTPASRSTF
                     AAIPLEDQGITLGALQVSRSKGPEFTDDELNLLQGLGHIISVNLFASHRAEVEQFRLR
                     QLNLVREVSAQIANVLDVDDLASQVTRLIQKTFNFYYVAFFTLDPQDNALHFRASAVA
                     PRKGRRKASIALEVEIGQGLIGEAAKTGMEIVCDDVQSDSRYRFIDSLPETRSEVVIP
                     LKIEDHILGALDVQSNQPSAFHPIDLLILRALADNVARAVDGAKLYSALSRRANQLTL
                     VSEVSKSVTSTLDLSKLMRDAASLIHEKFGYPHVSLFTVHPNRRMIMYEAGSGKRSKK
                     LEGFTIPLDDPKGIMPWVARHGKTALANDVSKDSRYIQSPLPPKNTKSELCVPLIFNE
                     RVVGLLDIQSDQLNAFTEDDRVMFEAVADTMAAAIRNADLYRSEQWRRQVADSLRDVA
                     GLVSSNAGLDEVLEMILAELDRNLPIDVSAIWLLQEGELCLSAVHGIDGAQLENTCLA
                     FPESTYVMAEALESDVPIIRRPEDPMGPAGLTMNFDPTYSSIVAPLRVGDRPLGVLAL
                     AHHTSGRYGHEARAIAGTFANYAAVAMENTRLFDAAQEQAYASAALLQVAQAVVSLSD
                     LDEILGSIIRIMPILVGVKRVVLYRLDMEYQTLQASHEYGFSEEEEAVITEKILPVAD
                     FPLIVWALEQNILVTYPLKAEATPLDWFAIQPGSEEEAHSIDADRLLIAVPLTIKHDI
                     FGVLLIEEDENARRFRSRRLEIINGIAQQAALAIQNDLLQQEMVVRERLETEVQLARQ
                     IQQTFIPSILPTHPNWQIAARWRTARQVGGDFYDVIELPNNRLGIFIADVADKGMPAA
                     LFMALTRTLMRAAILETSSPAEALRRVNDSLLPDTQQGMFITAVYGELDLDQGTFTYV
                     NAGHNPPFWVKTDGSFEKLTRTSIALGVMEQPSIEQRVISFNPGETLLLYTDGLTEAF
                     SPSGDLFGEVRLLDVMASLTAGTAEEVIVSIEECLNDFVDTEPLADDLTMLAIRMAG"
     gene            complement(3401..3808)
                     /locus_tag="PLR93_05435"
     CDS             complement(3401..3808)
                     /locus_tag="PLR93_05435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH26440.1"
                     /translation="MPAYTFPAKFEFLDEIRDTVAEIARSGGFSEKTIYSLQLAADEA
                     ASNIIEHAYEGVSNATLYMTCDMKGDEIVITMRDTGKSFNPTTVKEPNLKADLSERQI
                     GGLGVYLMRKLMNTVHYESSKSGNLLTMTKRRE"
     gene            complement(3878..4213)
                     /locus_tag="PLR93_05440"
     CDS             complement(3878..4213)
                     /locus_tag="PLR93_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH26441.1"
                     /translation="MEITTQEFKHCDLIAVKGRVDSATAPQFAKALETANDNGRFKLA
                     LDMSELEYMSSAGFRALLAAQRNCKRYNRGEVVLVRVPDRIREALELAGFTELFKTFD
                     DTVTAVGSF"
BASE COUNT         1105 a         1054 c         1031 g         1082 t
ORIGIN      
        1 taatgtttgg ctaacatttc tgttagtgcg gtatttgtgt catggaatat gaccagatta
       61 ttgaatttca catttaaaca aaatcgcctc tgatttctca gaggcaattt tgtttaagaa
      121 aaaggaatag ccctaccctg ccatacgaat tgccagcatt gtcaaatcat cggcaagggg
      181 ttcggtatcg acaaaatcat tgaggcattc ttcaatcgat acaatgacct cttctgccgt
      241 gcctgcggtc aacgatgcca taacatctaa caagcgaacc tctccaaaca agtcaccgct
      301 tggggagaag gcttcagtga gaccatctgt gtagagcagg agcgtctcgc cggggttgaa
      361 ggaaatcacc cgttgctcaa tcgaaggttg ttccatcaca cctaaagcaa tcgatgtacg
      421 tgtcaacttt tcaaaactgc catcagtctt cacccaaaat ggcggattat gaccagcatt
      481 tacataggta aacgtacctt gatccaaatc caattcgccg tacacagcag tgatgaacat
      541 tccttgttgt gtgtcaggca ataacgaatc attcacgcgt cgcaatgctt cggcaggaga
      601 agatgtttcc aaaatagcag cacgcatcag ggtacgtgtc aacgccataa acaaagcggc
      661 gggcatgcct ttatcagcaa catcagcaat aaagatgccg agcctattat tgggcaactc
      721 gatcacatcg taaaaatcac cgcccacttg gcgtgctgtg cgccatcgtg ctgcaatctg
      781 ccagttggga tgagttggca aaatgctcgg gatgaaggtc tgctgaattt ggcgagccag
      841 ttgaacttcc gtttcgaggc gttcgcgcac caccatctct tgttgaagca aatcgttctg
      901 aatcgccagc gctgcttgtt gagcaatgcc attgataatt tccaaacggc gtgagcggaa
      961 ccggcgcgca ttctcatcct cttcgatcaa gaggacgccg aaaatatcat gcttgatggt
     1021 taacggcacc gcgatcaaca aacgatccgc atcgatgcta tgcgcttctt cctccgaccc
     1081 aggctgaatc gcaaaccaat ccaacggcgt cgcctcagcc ttgagtggat aggtcactaa
     1141 tatattttgc tccagcgccc acacaattaa tggaaaatca gcaacaggca aaattttctc
     1201 ggtgataacg gcttcctctt cttcagaaaa accgtactcg tgcgaagctt gtaaagtttg
     1261 atattccata tcaaggcggt acaacacaac tcgtttgaca cccaccaaga tcggcataat
     1321 acgaataatg gagccgagaa tttcgtccaa atcgctcaaa ctgacaacag cttgtgccac
     1381 ttgcaacaaa gcagcagagg cataagcctg ctcttgagca gcatcgaaga ggcgggtgtt
     1441 ttccatagca actgccgcat aatttgcgaa agtacccgca atcgcacgcg cttcatgtcc
     1501 ataacgccca gatgtgtggt gagccaatgc caagacgccc aatggacggt cacccacccg
     1561 caatggcgcc acaatggatg aatacgttgg gtcaaagttc attgtcagcc cagcgggacc
     1621 cattgggtct tcagggcggc ggatgatagg aacgtccgac tcaagcgcct cagccatcac
     1681 atacgtagat tcaggaaatg ccagacaggt attttcaagt tgtgcaccat caatgccatg
     1741 cacagccgag agacacaact caccctcttg aagcaaccaa attgcagaaa catcaattgg
     1801 aagattgcgg tctagctcag caaggatcat ttcaagcact tcatccaacc cagcattgct
     1861 ggaaaccaag ccagcgacat ctcgcaaact atcggcgact tggcgacgcc attgctcaga
     1921 acgatataag tctgcattac ggatggcggc agccatggta tctgctaccg cttcgaacat
     1981 gacccggtca tcttctgtga atgcattgag ttggtctgac tgaatgtcca acaaaccaac
     2041 aactcgttcg ttaaaaatca acggaacaca taactctgac ttcgtatttt ttggcggcaa
     2101 aggcgattgg atgtaacgcg agtcttttga aacgtcattc gcaagcgcgg tttttccatg
     2161 gcgtgccacc caaggcataa tgcctttcgg gtcatctaat gggatggtga agccttcaag
     2221 ttttttgcta cgttttccac ttcccgcttc gtacataatc atgcgccgat tcgggtggac
     2281 ggtgaaaagc gaaacgtgag gataaccaaa tttttcgtga atcaagctag cagcatcgcg
     2341 cattagcttt gaaagatcga gcgtagatgt aacactttta cttacttcag aaaccaaggt
     2401 cagttgattg gcgcgtcggc tcagggcact atagagtttg gctccatcta cagcgcgggc
     2461 aacattatcg gcaagggctc gtagaatgag taagtcaata ggatgaaaag cgcttggctg
     2521 attgctctga acatccaatg cgcccagaat atggtcttct atcttgagcg gaatgacaac
     2581 ttcagagcga gtttctggga ggctatcaat aaagcgataa cgagaatccg actgaacatc
     2641 gtcacagacg atctccatgc cggtctttgc tgcttcaccg atcaacccct gaccgatctc
     2701 cacttccaat gcaatggatg ctttacggcg acctttacgc ggtgcaacgg cgctagcacg
     2761 aaagtgaagc gcattatctt gagggtcgag agtgaaaaag gcaacgtaat aaaagttaaa
     2821 ggtcttttgg ataagtcttg ttacttgaga tgctagatcg tccacatcga gcacgttggc
     2881 gatctgagcg ctgacctcgc gtaccaagtt caattggcgc aagcggaatt gttccacttc
     2941 ggcgcggtgc gaggcaaata aatttacaga gatgatatgc cccaagcctt gaagcaggtt
     3001 taattcatca tctgtaaact ctggtccttt tgagcgggat acttgaagcg ctcccagcgt
     3061 aatcccctga tcttcgaggg ggattgcagc aaaggtcgag cgagaggcgg gggttttctt
     3121 cccgctgctt tttttcttac gagccagttt ccccgttttt atggcacgtt tcattccgtc
     3181 taatgcaggc tgggttggga agatatatcc atcgtcccaa tctggcaggc ggaagatgtt
     3241 ttcgtgcagc cagacatcca ccttgccttc gatgaggcgg tttgccatag cagcgatgcg
     3301 atctcgttgc gcgctaagag agtcttcgct ccgcagttgc tcacccaagc tggcgagttg
     3361 aagccaatcc ttagaggatc gggttgcggc gctcatgaaa ctattctctc cgtttggtca
     3421 ttgtcagtag attgccagat ttggaagatt cgtaatggac ggtgttcatc aacttccgca
     3481 tgagatatac gcccagcccg ccgatctgcc gttctgatag gtctgctttg aggttcggct
     3541 ctttgaccgt ggtagggttg aatgactttc cagtatctcg catggtgatg acgatctcgt
     3601 cccctttcat atcacacgtc atatacaaag tagcgttcga cacaccttca taggcatgtt
     3661 caatgatgtt agaagcagct tcgtccgcgg caagttggag ggagtaaatg gtcttttcag
     3721 agaaccctcc gctgcgcgca atctctgcaa ctgtatcgcg gatctcgtcc agaaattcaa
     3781 acttggctgg gaatgtgtat gctggcataa agaaaatgag atgccgtccc ttgtctttaa
     3841 agagacggca tgtcggttct ttttaggaac ccttctatta gaaacttccg acggcggtta
     3901 cggtgtcgtc gaaggttttg aagagttcgg tgaagcctgc caattcgagg gcttcgcgaa
     3961 ttcggtctgg aacacgaaca agcaccacct cgccgcgatt gtatcgtttg cagttgcgtt
     4021 gtgctgcgag caaggcacgg aaacctgcgc tggacatgta ttccaattcg ctcatatcaa
     4081 gggcaagttt gaaacgtccg ttatcgttgg cagtttcaag tgccttggca aattggggag
     4141 ctgtcgcgct gtcaacccgt ccttttactg caatcaggtc acagtgcttg aattcttggg
     4201 tcgtgatttc cataatttct cctccgaaca agatatcgag tgatgaatta gcgggattat
     4261 atcacgtaga ta
//
LOCUS       DAOQFT010000163         4270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11143_length_4270_cov_7.103915, whole genome shotgun sequence.
ACCESSION   DAOQFT010000163 DAOQFT010000000
VERSION     DAOQFT010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11143_length_4270_cov_7.103915"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..733)
                     /locus_tag="PLR93_05445"
     CDS             complement(41..733)
                     /locus_tag="PLR93_05445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH26442.1"
                     /translation="MNNQKQISESAQYWKDHLKSWEASAYFKEGQQNANWWDKLSTVF
                     RGDAMYVRMNTALELLQPHLSGKTILDVGCASGRFAFQMIQAGAQKVYGTDISSEALE
                     LARQHGKELGIQDKLDFSALDVVQPGNPLPQVDIVTALGVIEYFDAKDMASFLGNLRT
                     KYFLLDFPDYGRKKEFPTWILRQVYIRVNRLPGVYLYSLEEFSKIAEPFGFKKLRVIK
                     RNNFYYVTNLPE"
     gene            complement(776..1537)
                     /locus_tag="PLR93_05450"
     CDS             complement(776..1537)
                     /locus_tag="PLR93_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNH26443.1"
                     /translation="MTIAITRHISSRFNECEITHIERTPIDLEIARSQHEAYVRALAE
                     LGCQVIELPEETELPDSVFVEDTAFILPEVAVITRPGADSRKPEVESIIPALSPYRPL
                     LHVATPATVDGGDVLVAGKQIFVGMSTRSNREVVIQLNALLEDFGYKVWGVELRDCLH
                     LKTAVTRINDQTLLINKAWVDASNFPGYDLIEIDASEPFAANCLPVRGTIIYPTTFPK
                     TRNLLENKGFRIVPVDLSELAKAEGAVTCCSLIIE"
     gene            complement(1541..1909)
                     /locus_tag="PLR93_05455"
     CDS             complement(1541..1909)
                     /locus_tag="PLR93_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH26444.1"
                     /translation="MATPGFKITSEEIQHNDKSVTVFHLSGWVNVQSEEKVFSTAETA
                     YTNGSRYLVVNLEDVQMLTSAGIRLLQRIYKLYRSTETIGLKICSAPSQVYHALALTG
                     LLQTVPMYENLQAALSAYDL"
     gene            complement(1925..2680)
                     /locus_tag="PLR93_05460"
     CDS             complement(1925..2680)
                     /locus_tag="PLR93_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNH26445.1"
                     /translation="MVKVTNLDKYFGRLHVLKDISLEVPERQVLCLIGRSGSGKSTLL
                     RCLNFLEDPSRGIIEVDGIKVDAEVKNKERRQLIHDVRLKTGMVFQEFNLFPHMTVLE
                     NVIEGPVTVKGMDKKVATELAEQNLDSVGMLFKKDEYPLRLSGGQKQRVAIARALTMQ
                     PRVMLFDEPTSALDPELIGEVLNVMKKVAYEGMTMIVVTHEMGFARDVADRVIVMSEG
                     QLIEDATPEALFTSPKDPRTKALIDRYRSDDQQ"
     gene            complement(2697..3545)
                     /locus_tag="PLR93_05465"
     CDS             complement(2697..3545)
                     /locus_tag="PLR93_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNH26446.1"
                     /translation="MASTTNQEQAPLSQAELLYQAQLRKERLFRTHVGVSWAVLFFIL
                     IFLFSGQTIQLGSFKFDTIELDSSFILKNLSFIAQGLGQTLLISVLSILFAMILALLA
                     ALGRLSTFPPIYAISTFYVSLIRGTPLYLQIFFFFLALPQLGIILSGIVAGVMALGLN
                     YGAYMSETFRAGLSSVGKGQREAAMALGMTPGQTMRRVILPQALRFAIPPMGNDFISM
                     TKDSALVSATGFVHELMWRATKVGRAQFNNLEALIMAAMFYWILTLILTYFQGKLEAR
                     LAKGDR"
     gene            complement(3623..>4270)
                     /locus_tag="PLR93_05470"
     CDS             complement(3623..>4270)
                     /locus_tag="PLR93_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNH26447.1"
                     /translation="IGDSLGVETCFATPSWDAITAGNWADKWDMSVGSMTVTTARQQI
                     LDFSVPYYYTPAVVAVAADAGIDSLEGLSGQSVCAGAATTYEAWLNNDTEALGLPASS
                     IYAQVPADVTVVPLETDQECAQAIASGRKDFVAYVTSETVVDANLAAGMPVVKLDGAV
                     YSEDLAAAFDKSSSLDTASLRAKVDELFKAMHEDGRLSELSNKWFEVDLTAAPNQ"
BASE COUNT         1125 a          970 c         1106 g         1069 t
ORIGIN      
        1 tgatccggtt ccatgtccaa agtaacgcag gctagttttc tcattcaggg agatttgtaa
       61 cgtagtagaa gttgttgcgt ttaatcacac gcaacttctt gaaaccgaat ggttcggcaa
      121 tctttgagaa ttcttccagt gagtataagt acacgccggg taatcgattt acacgaatat
      181 aaacttgtcg caaaatccaa gtgggaaatt cctttttgcg accataatca gggaagtcta
      241 acaagaaata ttttgtgcgt aaattgccaa ggaaagaagc catatctttg gcatcgaagt
      301 attcaatcac tccaagagcg gtgacaatat caacttgtgg gagcgggttg cctggttgaa
      361 caacatcgag agccgaaaaa tccagcttat cctggattcc aagttctttg ccgtgttggc
      421 gggcaagctc aagcgcttca ctggagatgt cggtaccata tactttctga gcgcctgcct
      481 gaatcatttg aaaggcaaat cgacctgagg cacagccaac atcgagaatg gttttcccag
      541 agaggtgcgg ctgaagcaac tcaagcgcgg tattcatgcg cacgtacatt gcatcgccgc
      601 gaaaaacggt tgaaagctta tcccaccagt tggcgttttg ctggccttct ttaaagtatg
      661 cgctggcttc ccaacttttg agatggtctt tccaatattg agcagattcg gaaatttgtt
      721 tttgattgtt catggtgagg gattataccg cttcacccca gaccttgagc ctgattcact
      781 caataatcag gctgcaacat gtgacggcgc cttctgcctt tgccagttcg cttaggtcca
      841 cgggaacaat gcgaaagcct ttgttttcaa ggaggttacg ggttttagga aatgttgtcg
      901 ggtagatgat tgttccgcgg acgggcaggc agtttgcggc gaaaggttct gaggcgtcga
      961 tctcgatcaa gtcatagccg ggaaagttcg atgcatcgac ccaggctttg ttgatcaaca
     1021 gcgtctggtc atttatgcgc gttacggctg tttttaaatg taaacagtct cgtaattcca
     1081 caccccatac cttgtaacca aaatcttcaa gcagggcatt caactgaata acaacctcgc
     1141 ggttgctacg tgtggacatg cctacgaaga tttgtttacc tgccaccaac acatcgccac
     1201 cgtccacggt ggcaggggtt gccacatgca gcagtggacg atacggcgat agtgctggga
     1261 tgatagactc gacttcaggt ttgcgggaat cagcaccggg tctggtaatg acagcgactt
     1321 caggcagaat aaaagctgta tcttcaacga agactgaatc aggaagttct gtctcttcgg
     1381 gaagctcaat gacctgacag cccaactcag ctagtgcacg aacatacgcc tcatgctgcg
     1441 aacgtgcaat ttccaagtct atgggtgtgc gttcaatatg agttatttca cattcgttga
     1501 aacgagaact aatatggcgg gtaatagcaa tggtcatgat ttagagatcg tatgcagaaa
     1561 gcgcggcttg aaggttttca tacataggca cggtttgtag caagccagtt agagccaacg
     1621 catgatagac ctgagatggg gcgctgcaaa tcttcaaccc gattgtttcg gtagaacgat
     1681 atagtttata gattctttgt agcaggcgta tcccagcgct ggtgagcatc tgtacatctt
     1741 ccagattgac aacaagatag cgggagccat ttgtgtaggc tgtttctgct gtagaaaaaa
     1801 ctttttcttc gctttgtacg ttgacccagc cagacaagtg gaatacagta acagatttgt
     1861 cgttatgttg aatttcttcg cttgttatct tgaaacccgg agttgccatg aaaaagatgc
     1921 cttattattg ttggtcgtcg gaacgatacc ggtcgatgag ggctttggtg cgagggtcct
     1981 taggactggt aaacaacgct tctggggtgg catcttcgat caactgacct tcagacatca
     2041 caatgacacg atctgccaca tctcgggcaa agcccatttc gtgtgtgaca acgatcatag
     2101 tcattccttc gtaagccact ttcttcatca cattcaatac ttcgccaatg agttccgggt
     2161 ctaaagcaga ggtcggttca tcgaagagca tcacacgcgg ttgcatagtg agggcacgtg
     2221 caatggcaac acgctgtttt tgcccgcccg ataggcgtag cggatactcg tctttcttga
     2281 aaagcatgcc cacactatcc aggttttgtt ctgcgagttc agtggcgact tttttatcca
     2341 tgccctttac agttacagga ccttcgatca cgttctctaa tacggtcatg tgtgggaaga
     2401 ggttgaattc ttgaaacacc atgccggtct tcaaacgcac atcgtgaata agttggcgac
     2461 gttctttgtt cttgacttca gcatctacct tgatgccatc tacttcaatg attccacgag
     2521 agggatcttc gagaaagtta aggcaacgca gaagcgtact tttccctgaa ccgctacgtc
     2581 cgataaggca tagaacctga cgttcaggca cttcaaggct aatatctttc aatacatgca
     2641 aacgcccgaa atatttatcg aggtttgtaa cttttacaat tggaagggtg gacatattat
     2701 cggtctccct ttgcaaggcg ggcttccaat ttgccttgga agtaggtgag gatgagggta
     2761 aggatccaat agaacatggc tgccataatg agcgcttcaa ggttgttgaa ttgagcacgt
     2821 cctactttgg tggcgcgcca catcaattcg tggacgaaac ctgtcgccga gacgagagcc
     2881 gagtctttcg tcatggagat aaagtcgttg cccataggtg gaatggcaaa acgcaaagcc
     2941 tgcggtagga tcacacgcct catggtctga ccgggagtca tgcccagcgc catggcagct
     3001 tctctttgcc ctttgcctac cgagcttaag ccggcacgga atgtttcaga catgtaggca
     3061 ccataattca gacccaatgc catcacccct gcgacgatac cggaaagaat aatgccaagt
     3121 tgtggcaagg caaggaagaa gaaaaatatt tgcagataaa gcggcgttcc acggatcagc
     3181 gatacgtaga acgtgctaat ggcataaata ggagggaagg tggaaagcct gccaagggct
     3241 gccaaaagag ctaaaatcat tgcaaacaaa atagagagga cggagatcaa aagagtttgc
     3301 cccaaccctt gtgcaatgaa agatagattt tttagaataa aactgctgtc tagttcgata
     3361 gtgtcgaact taaaagaacc aagttgaatt gtttgcccac tgaaaagaaa gatcaagata
     3421 aagaacagaa cagcccagga aacgccgaca tgcgtgcgaa acaaacgttc cttgcgaagt
     3481 tgtgcctgat aaagcaactc ggcttgtgaa agcggagctt gttcctggtt ggtggtggaa
     3541 gccatctttt acctccgaga ggtgaagtgg aataataaaa agagaagcgg agatgatccg
     3601 cttccctttt tatttcgttt tcttactggt tgggtgcagc ggtcaagtca acttcgaacc
     3661 acttgttgga gagttcggag aggcgaccat cttcgtgcat ggctttgaag agttcgtcca
     3721 ccttggcgcg cagggaagcg gtgtcaaggg aagaggactt gtcgaaagcg gcagcgaggt
     3781 cttcagagta gacagcgccg tctaacttga cgacaggcat gcctgcggcg agattcgcat
     3841 ccacaacggt ttcagaggtc acgtaggcaa cgaagtcctt acgaccagag gcaatcgcct
     3901 gggcgcattc ctgatcggtt tcgagaggaa ccacggtgac atcagcagga acctgagcgt
     3961 agatggagct ggcgggcaag ccaagcgctt ctgtatcgtt gttgagccag gcttcgtagg
     4021 tggtggcggc gccagcacaa acggactgac cggaaagacc ttcgagagag tcgatgccag
     4081 catcagcagc aacagccaca accgcggggg tgtagtagta gggcacgctg aaatcgagga
     4141 tctgttggcg agcggtggtg acggtcatgg agcccacgct catatcccat ttgtctgccc
     4201 agttaccggc agtgatcgca tcccaggagg gagtagcgaa acaggtttcc acaccgagtg
     4261 agtcaccaat
//
LOCUS       DAOQFT010000164         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11228_length_4249_cov_6.206247, whole genome shotgun sequence.
ACCESSION   DAOQFT010000164 DAOQFT010000000
VERSION     DAOQFT010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11228_length_4249_cov_6.206247"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2174
                     /locus_tag="PLR93_05475"
     CDS             <1..2174
                     /locus_tag="PLR93_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNH26448.1"
                     /translation="WLVVLYALLAMVINIAEAIWQDRGDNSFLFQELQTYISFTLIII
                     LMITIQVFLKRELWWAWLAYFGVWVIGLALILTNALNLPDTLWTNGELILYRSRLGPA
                     WVMLGWLILSIVMILNLFNAQHQSRQPLLRSRLGYWWAPVIFTLVNDILLFSGILIIG
                     QPVRLLVAIGMAYVVGTHYVPDLKNITRRAMIYVASTIGIVGFYIAGFLIIQALFGGN
                     PSYSPLLAGAVVALFIALIFAPLTDLVSRAINIWTKGDQYDASLTIHQYSESISNILD
                     MDRLASIAIGIMLEAMQIERGFLFLVDSERQADGRKTYKIRSARSPEERQLVAVELEE
                     DGLIASYFIREQKPLLQYDLDLLPAFRAASTVEKNWFTELRSEVYIPIFAKKQWIGLL
                     AFGSKLSGNRYTREDLSVLSAHANQTAVALENARLVDNLMRLNQELRQARRTLEKNNQ
                     ELQRIDQAKSDFISIASHELRTPLTVIKGYTEMLMDDPKLEKGQKSMMKGIHDGTIRL
                     HEVMDSMFDIAQIDARSLKPHLQPVDVGQLIHQVCLEHAKTVKEREQILTIKAQAIPL
                     VKADSHLLEKLFHHLIRNAIKFTPNNGWITITAKHIPAIINLPNGGIEIIVSDTGVGV
                     DPNLREVIFSKFYQPGELGKHSTSKSRFKGNGAGLGLALSKGIIEAHGGRIWVESPGY
                     DEVNFPGSQFHIILPLTKLENGEVQRTSEPLTIELGKAKEA"
     gene            complement(2218..3348)
                     /locus_tag="PLR93_05480"
     CDS             complement(2218..3348)
                     /locus_tag="PLR93_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26449.1"
                     /translation="MLVIEKVDTENKKQVKRFVELPPRLYADCPQWVPPLNVDAYNQL
                     NRKKHPFHEHSDVDFFLAVRDGLDVGRIAVIENKPFNQYHQEKTADFYLFECEDDLEA
                     ATALFNTAFEWAKSRGLTKMVGPKGMGPLDGYGVLVFGHEHRQTMTMLNYNHAYYQGL
                     VEAQGFTKEVDFVSCYLPADKFQMPERVKKISERVMQRGHLEIKTFKSKKDLLSWADR
                     IGTAYNNAFVNNWEYYPLSQREIDFVVENIMTIADHRLIKIIVHEQDVVGFLFAFHDV
                     SAAMQRAKGKLFPFGLLGILMELRRTNTVAVNGMGILPEFQGHGGNALLYYAMGDTLL
                     NFGQFTHVEMTQVAETTEQMRADLKNLNGVEYKNNRVYRKLL"
     gene            3507..>4249
                     /locus_tag="PLR93_05485"
     CDS             3507..>4249
                     /locus_tag="PLR93_05485"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNH26450.1"
                     /translation="MTGTTHQQMLAKYAEAIVKVGLNIRAGQRLIITAAGTRGVPNQF
                     APLVREITKAAYGVGAKYVDVIFADEEMMRIRAQHAPKGSFGEYSKWQIQGILDMINN
                     GDALLSIGGSNPDLLGGLDPEVVGQMQRAHLENWSPISEKVTTNAINWCVVAAAGEAW
                     AHKIFPDLPLNQAQEKLWQAIFETTRIDQPDPVGAWSKHIASLRERALYLQSKQYTAL
                     HYTAPGTDFTVGLPSGHLWISAQSMAQNGV"
BASE COUNT         1124 a         1085 c          935 g         1105 t
ORIGIN      
        1 cttggttggt tgttttgtat gccttacttg caatggtgat caacattgcc gaagccattt
       61 ggcaagaccg tggggataac tcttttcttt tccaagaact tcaaacgtat atttcgttca
      121 ccctgatcat cattttgatg atcacgattc aagtcttctt gaagcgcgaa ttatggtggg
      181 cttggctggc gtattttgga gtttgggtca ttgggctggc attgatcctt acaaatgctt
      241 taaacctgcc agatacttta tggacaaatg gtgaattgat cctgtatcgt tcgcggttgg
      301 gtcctgcttg ggtcatgttg ggatggttaa ttttatccat cgtgatgatc ctaaatttat
      361 tcaatgcgca acaccaaagc cgccagccgt tgcttcgcag ccgattggga tattggtggg
      421 cgccggtcat tttcactctc gtcaacgaca ttcttctttt ctctggaatt cttatcattg
      481 ggcaaccggt gcgcctgttg gttgcaattg gcatggcata cgtagtaggc acacactacg
      541 tgccagacct aaagaacatc actcgccgtg ccatgatcta tgttgccagc acgatcggca
      601 ttgtaggctt ttacattgct ggctttttga tcatccaagc tttattcggt ggcaacccaa
      661 gttatagtcc gctattggca ggcgccgttg ttgcgctttt cattgcgttg atcttcgcgc
      721 cactcacaga cttggtctca cgcgccatca atatctggac gaaaggcgat caatacgacg
      781 ccagcctcac cattcaccag tatagtgaaa gcatcagcaa catcttggac atggatcgcc
      841 ttgccagcat tgccattggc attatgctgg aagccatgca aattgaacgc ggcttcctat
      901 tcctcgttga ttctgaacgt caggcagatg gtcgcaagac gtataaaatt cgttcggcac
      961 gtagccctga agaacgacaa ttggtcgccg tggaattgga agaagatggg cttatcgctt
     1021 cgtattttat tcgggagcaa aaaccgcttc ttcaatatga cctggacctg ttgccggcgt
     1081 ttcgagctgc ttctaccgtt gagaaaaatt ggtttacaga attacggtca gaagtttaca
     1141 ttcccatctt tgccaaaaaa caatggatcg gtctgctggc attcggttca aaattaagcg
     1201 gcaatcgtta cacccgagaa gacctttccg ttcttagcgc acatgccaac cagacagccg
     1261 ttgcgcttga aaacgcccgc ctcgtggaca atctcatgcg cttgaaccaa gaattacgtc
     1321 aggcgcgccg tacgcttgaa aagaacaatc aagaattaca acgcattgac caggcaaaat
     1381 ctgatttcat cagcattgca tctcatgaac ttcgtacacc gctcaccgtc atcaaaggct
     1441 acaccgaaat gctaatggat gacccaaagc tagaaaaggg tcagaagtcc atgatgaagg
     1501 gcattcacga tgggacgatc cgtctccacg aagtgatgga ctcgatgttc gacatcgctc
     1561 agatcgatgc acgctcgctc aagccccatc ttcagcccgt ggatgtgggt caattgatcc
     1621 atcaggtatg tctggaacat gccaagaccg tcaaagaacg cgaacagatt ctaaccatca
     1681 aagcccaagc cattccgctc gtcaaagcgg actcgcacct cttggaaaag cttttccacc
     1741 acttgatccg caatgccatc aagttcacgc cgaacaatgg ctggatcacc atcaccgcca
     1801 aacacatccc agccatcatc aacctcccca acggtggcat cgagatcatc gtcagcgata
     1861 ctggtgtggg cgtagacccg aacttgcgcg aggtaatctt ctcgaagttc taccagccag
     1921 gtgaactggg caagcattcc actagcaaat cacgcttcaa gggcaatgga gccggtttag
     1981 ggctggcact ctcaaaaggt attattgagg cgcatggtgg tcgtatctgg gtggaaagcc
     2041 ccggctacga cgaggtcaac ttcccgggca gtcagttcca cattatcctg ccgttgacca
     2101 agcttgaaaa tggcgaagtg caacgcacaa gtgaacctct caccatagaa cttggaaaag
     2161 caaaagaagc ataaaaaaag agcggagatt tataaatctc cgctcttttt atcagaatta
     2221 cagtaatttg cgatacacac gattattttt atattccacc ccattcaagt tctttaaatc
     2281 tgctcgcatt tgttcagtgg tctcagccac ttgtgtcatt tcaacatggg tgaattgacc
     2341 gaagtttaat aatgtatctc ccatcgcata atacaacaag gcattcccac catgcccctg
     2401 aaactccggc aggattccca taccgttcac agctacagta tttgtacggc gaagttccat
     2461 caaaataccc aacaacccaa acggaaagag cttgcctttc gcacgctgca tggctgcaga
     2521 aacatcatga aacgcaaaga gaaaaccaac cacatcctgt tcatggacga tgatcttgat
     2581 caggcgatga tcggcgatag tcatgatatt ttccaccaca aaatcgatct cgcgttgtga
     2641 gagtggataa tactcccagt tattcacaaa agcgttgttg taagcggtgc caattcgatc
     2701 cgcccaactc aacaagtcct ttttgctctt gaatgttttt atctctaaat gacctcgttg
     2761 cataacacgt tcagagatct ttttaactcg ttcaggcatt tggaatttat ccgcgggtaa
     2821 ataacaagag acaaagtcca cttcttttgt gaaaccctgc gcttcaacca atccctgata
     2881 ataagcatgg ttgtaattga gcatggtcat ggtctgacgg tgttcatgcc cgaaaaccaa
     2941 cactccatac ccatctaacg gacccatccc tttcggtcca accatcttgg ttaatccgcg
     3001 tgattttgcc cactcaaacg ctgtgttgaa caatgccgta gccgcttcaa ggtcatcctc
     3061 acactcgaat aaataaaaat ctgccgtctt ttcttgatga tattggttga aaggtttgtt
     3121 ttcaattaca gcaatccgcc ctacatccag tccgtcacga accgctagga aaaaatccac
     3181 atcagaatgt tcatggaagg gatgcttctt tcgattgagc tgattgtacg catccacgtt
     3241 caatggcggc acccactgcg ggcaatccgc atagagacgg ggtggtaact ccacgaaccg
     3301 tttgacctgc tttttattct cggtatccac tttctcgata acaagcatgg ttttgctcct
     3361 atagaggcta ggtttcagcc aagtatatcc cttccacccc attttaaaag gtgggtgtgc
     3421 attgcttttg taatggatat ccctcgtgca aaaacccctg tcgccatgct atacttgtaa
     3481 ctatctccca aattaactga ggatttatga caggaactac ccaccaacaa atgctggcaa
     3541 aatatgccga agccattgta aaagtaggtc taaatattcg cgccgggcaa cgtttgatca
     3601 tcacagccgc agggacgcgc ggtgtgccta accaatttgc accattagta agagaaatta
     3661 ccaaagccgc gtatggtgtt ggcgcaaaat atgtagatgt catctttgca gatgaagaaa
     3721 tgatgcgcat ccgcgctcaa catgccccga aaggcagctt tggagaatat tccaaatggc
     3781 aaattcaagg catcttggac atgatcaaca atggcgatgc cttgctctcc atcggcggct
     3841 caaaccccga cctacttggt gggcttgacc ctgaagttgt tgggcagatg caacgagctc
     3901 atttggaaaa ttggagtccc attagtgaaa aagtgaccac caatgccatc aactggtgtg
     3961 tagttgccgc cgctggtgaa gcatgggcgc ataagatttt cccagacctt cctctgaacc
     4021 aagcgcaaga aaaactttgg caagccatct ttgaaacaac tcgtattgac caacccgacc
     4081 ccgttggtgc atggagcaaa cacatcgcct ctttacgcga acgggctttg tatctgcaat
     4141 ccaagcaata cacagcgttg cattacacag cccccggcac cgactttacc gtcggcttgc
     4201 ccagcggaca tctctggatc agtgcccaat cgatggcgca gaatggtgt
//
LOCUS       DAOQFT010000165         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11246_length_4246_cov_6.690050, whole genome shotgun sequence.
ACCESSION   DAOQFT010000165 DAOQFT010000000
VERSION     DAOQFT010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11246_length_4246_cov_6.690050"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLR93_05490"
     CDS             complement(<1..756)
                     /locus_tag="PLR93_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26451.1"
                     /translation="MTHRTFSPRNLLFFLASFLAFVSIACATTAPSTSTSPTAVVDVP
                     ATATAEILIPVTGGGDEAAPALPEFRRLTLEYPPVMKVGSGSDVIILTLEVDEQGNIT
                     PTAQIDGNVTQGEVVEIPNLYATHNVTAEAYYEVSGLTVTPKGSTFQPLKQGERIRFT
                     WSVQPQEVGTYGGTIFLFLNFENRETGEKDRKEISIQIFDIKVVDFFGFSTNFVKTSG
                     VVGSVLGVIVGFPFFDDIVKYLLGRLTSEKGKPG"
     gene            complement(753..926)
                     /locus_tag="PLR93_05495"
     CDS             complement(753..926)
                     /locus_tag="PLR93_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26452.1"
                     /translation="MKQIRLVLAIILLTISIALLVWGFSPNPHETKIQMIAPTEMQLP
                     TPSSLHLNPEPVA"
     gene            complement(923..1672)
                     /locus_tag="PLR93_05500"
     CDS             complement(923..1672)
                     /locus_tag="PLR93_05500"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNH26453.1"
                     /translation="MESLDLVQEMYRLVEAANAKKIQLRAIGGLAVQTHNKTDHPLFK
                     REFADLDFCIPLKQRREFEAFMPEMGYSPHRQFNVLNGDQRQIYYHNPTEMKVDIFVG
                     HFEMCHKIPLEERLNADPVTIPLAELFLSKAQIIELNRKDAFDLTSVLLNNVTGQGDN
                     ETINLKILAQLGSADWGLYKTTSINLDKVEQMLLKNEIPLNEEERAIVIGRINDIRRT
                     FAEMPKPVAWQLRDRVGTRVKWYIEVEEVQR"
     gene            complement(1711..2610)
                     /locus_tag="PLR93_05505"
     CDS             complement(1711..2610)
                     /locus_tag="PLR93_05505"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HNH26454.1"
                     /translation="MSVIEEIAARVEEWKGQNVVIDQLSGGLTNTNYKITVNGKPYFV
                     RVPGASTELLAIDRNNEYHNTSAAATAGVAPKVLQHFPEYHVMVIEFLDGKTMSKDSL
                     NAAGMPTRMAHAIKKLHAGPRFLTDFNMFRLTEYYLSLCRERAIKIPNGYPERMSTVN
                     EIEKAMSVKPLATVPCNNDLLAENYIDDGKQLWLIDYEYSGNNDPTFELGNTCQEMQF
                     NDDQIKEVCAAYFGDATPQMVARMKLNMIMSDVGWGLWAAIQANISTIDFDFWGWALE
                     RWGRAEEKMDSKEFSVWMKDVVQ"
     gene            complement(2682..>4246)
                     /locus_tag="PLR93_05510"
     CDS             complement(2682..>4246)
                     /locus_tag="PLR93_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNH26455.1"
                     /translation="INLVTLGFYSFSQMYYFGGGMIPALIISAIFIIFEVVVYASLIA
                     VMPRSGGDYVWQTRVFGGGIGFILSITGWWFTLWLWTPIYGDMLRQIVLTPLLGAFGF
                     QQAAVWIAGSGTPLFVCSLLTLLFVALVIFLGMKSYARVQKYSFYAGMLGLLIVLVLL
                     FTGSPEKFKAGLDANSASYFGTSGSLYEPTAQAGVDAFASTPLMGGSFGLIMVTLPYL
                     VFFNLWPNWGATLYGEVRGATDYKRNFSGMAWALVATTMLGIIFFLGVQKTIGWDFYV
                     QSNAAWWNYAWGYVTEAPPLPVWPNPAMLAVFLTDNRLIQIIVLLLMSTWWFGWAGTL
                     FLSSTRVIFAAAFDRLLPEKAAELNDRGTPVNAMLYMILPAILVAALYAYDVAGFKSI
                     TLAATQGIAIMYFGTAIAAIILPYKKKELYEASPIAKMKVAGIPLITVAGLIFGGFLL
                     FLLVEWFFDPWLNSAGVPPGLYGISIANTNSMIFLGSCYAIAAAIYYGFKSKRKSEGI
                     DMDKVTAEIPVE"
BASE COUNT         1112 a         1052 c         1093 g          989 t
ORIGIN      
        1 gcccggcttg cctttttctg aagtcaaacg cccgagcagg tacttgacga tatcgtcaaa
       61 aaatggaaag ccaacaatca cacccaaaac cgagccgacc acaccagagg tcttcacaaa
      121 attcgtagaa aagccaaaaa agtccaccac tttgatgtca aaaatttgga tggaaatttc
      181 tttgcggtct ttttcgccag tctcgcggtt ttcaaaattc aagaaaagga aaatcgtgcc
      241 gccgtatgtc cccacttctt gaggttgcac actccatgta aaacgaatgc gctccccttg
      301 tttcaacggt tgaaaagtag agcctttcgg cgtcaccgtc aaccctgaaa cttcgtaata
      361 ggcttcagcc gtcacattat gggttgcata aagatttgga atttcaacga cctccccttg
      421 cgttacattc ccatcgatct gagcagttgg ggtgatattc ccttgttcat ccacttccaa
      481 ggttaggatg atgacatcgc tgcctgagcc gaccttcatc acgggcggat attccaaagt
      541 taggcggcga aattctggga gggctggcgc cgcctcatct cctccgccgg tcactgggat
      601 gagaatttcg gcagtagcag tcgctggaac atccacaacg gcggtgggag aagtggaagt
      661 ggacggagca gttgtagcac aagcaatgct tacaaaagca aggaagctcg ccaaaaagaa
      721 caaaaggttg cggggagaaa aagttcgatg ggtcatgcaa caggctccgg gttgagatga
      781 agcgaagaag gcgtcggcag ttgcatttcc gttggggcga tcatctgaat ttttgtctca
      841 tgcgggttgg gagaaaatcc ccacaccagg agtgcaatgg aaatcgtcaa aagaataatg
      901 gcaagcacga gacggatttg tttcatcgct gaacctcttc cacttcaata taccacttga
      961 cgcgtgtgcc gaccctatcg cgtagttgcc aggcaacagg tttgggcatt tcagcaaagg
     1021 tgcggcggat atcattgatg cgcccaatca caatggcgcg ttcttcttca ttcaaaggaa
     1081 tttcattctt caacaacatt tgttccactt tatccaagtt aatggacgtg gtcttgtaca
     1141 aaccccaatc ggcactgcca agttgagcta ggatcttgag gttgatcgtt tcgttatcac
     1201 cttgcccagt gacgttgttc aacaaaacgg atgtgaggtc gaaggcatct ttgcggttca
     1261 gttcgatgat ctgcgctttg gaaagaaaaa gctcggcaag cggaatggtg accgggtcgg
     1321 cattgaggcg ttcttcgagc ggaattttgt ggcacatctc aaaatgtccc acaaaaatat
     1381 ccactttcat ttcggtgggg ttgtgataat agatctggcg ttggtcgcca ttgagcacat
     1441 tgaactgcct atgcggtgaa taacccatct cgggcatgaa cgcttcaaac tcgcggcgtt
     1501 gtttgagcgg gatgcagaaa tcgaggtcgg cgaattcacg cttgaacagt ggatgatcgg
     1561 ttttattgtg tgtttgtact gccaacccac cgatggcacg caattggatt tttttcgcat
     1621 tcgcggcttc cacaaggcgg tacatttcct gaactaggtc gagtgattcc atcaagatat
     1681 ttctcttctt ctcattttac ttatttcaac ttactgtaca acatctttca tccacactga
     1741 gaattctttc gaatccattt tttcttctgc cctgccccaa cgttcgagcg cccagcccca
     1801 aaagtcaaaa tcaatagtgg agatattcgc ctgaatggct gcccataaac cccagcccac
     1861 atcagacata atcatgttga gtttcatgcg cgccaccatt tgcggagtgg cgtcgccaaa
     1921 atacgccgca cagacttctt tgatctggtc gtcgttaaat tgcatttcct gacaagtatt
     1981 gcccaactcg aaggttgggt cgttgttacc actgtattca taatcgatca gccaaagctg
     2041 tttgccatcg tcgatgtagt tttcagcgag taggtcgtta ttgcaaggaa cggttgccaa
     2101 gggtttaacg gacatggctt tttcgatctc gttcacggta gacatccgct cgggatagcc
     2161 attggggatt ttgatagccc gttcacggca caggctgaga taatactccg taaggcggaa
     2221 catgttgaag tccgttaaga agcgcggacc agcgtgcagt tttttaatgg cgtgtgccat
     2281 gcgtgtgggc atacccgccg cattgagcga atcctttgac atggtcttgc cgtcgagaaa
     2341 ttcgatcacc atgacatgat attcagggaa gtgttgcaac actttcggcg cgaccccggc
     2401 tgtggcggcg gcactggtgt tgtgatattc gttgttgcgg tcaatggcaa gaagctcggt
     2461 gctcgccccc ggcacgcgga caaaatacgg tttgccattg acagtgatct tgtaattcgt
     2521 attggtcagt ccacccgaaa gttggtctat gaccacattc tgtcccttcc attcttccac
     2581 tcgggcggcg atctcttcta tcacagacat atctgtctcc taaacgcaaa aggtgacagt
     2641 cacttctaag ccaaagtgac tgtcaccttc ttttatagaa cctactctac tggaatttcg
     2701 gcagtgacct tgtccatgtc gatgccttca ctcttgcgct tggatttgaa gccgtaatag
     2761 atcgcagctg caatcgcata acaagagcca aggaagatca tggagttcgt attggcaata
     2821 ctgatgccgt acaaaccagg aggcacacca gcgctgttaa gccatgggtc gaagaaccat
     2881 tccaccaaga ggaagagtaa gaagccaccg aagatcaagc ctgccacggt gatgagcggg
     2941 atgcccgcta ctttcatctt ggcgatgggt gaagcttcgt agagttcttt tttcttgtag
     3001 ggcaggataa tggctgcaat ggctgtgccg aagtacatga tggcaatgcc ttgtgttgcc
     3061 gcaagcgtga tggatttgaa gccagccacg tcataggcat acaaagccgc caccaaaatc
     3121 gccggcagga tcatatacag catcgcgttg accggtgttc cgcggtcgtt caattcagca
     3181 gccttttcgg ggagcaagcg gtcgaatgca gcggcgaaga ttacacgggt ggaggaaagg
     3241 aagagcgtgc ccgcccagcc aaaccaccaa gtgctcatga gcaacaagac aatgatctga
     3301 atcaggcgat tgtcagtgag gaagacagcc aacattgccg ggttgggcca cacgggaagc
     3361 ggaggcgctt cagtgacata gccccaggcg taattccacc aggcggcatt ggattgaacg
     3421 tagaaatccc agccaatggt tttttgaacg ccgaggaaga agatgatgcc taacatggtg
     3481 gtggcaacca gcgcccaagc cataccagag aagttacgct tgtaatcggt agcaccgcgg
     3541 acttcaccat aaagggttgc gccccagttg ggccacaagt tgaagaagac caaatatggc
     3601 agggtgacca tgatcaaacc gaaggagccg cccatcaacg gggttgaggc gaaggcatcg
     3661 acaccagctt gagcagtcgg ctcatagagg ctgccagacg tcccgaagta ggaagcggag
     3721 tttgcatcca aaccggcttt gaatttttca ggtgaaccgg tgaagagcaa aacaagcacg
     3781 atcaacagac cgagcatgcc ggcgtagaag gaatacttct gcacgcgcgc gtaagacttc
     3841 atgccgagga agatgaccaa tgccacgaaa agcaaagtga gcaaggagca cacgaaaagc
     3901 ggagtgcccg agcctgcgat ccacacggcg gcttgttgga agccgaaagc gcccaacaat
     3961 ggcgtgagca cgatctgacg caacatatcg ccatagatcg gggtccagag ccacaaggtg
     4021 aaccaccagc ccgtgatgga aaggatgaag ccgataccgc cgccgaagac gcgtgtctgc
     4081 cagacgtaat cgccaccaga gcgaggcatg accgcgatca aggatgcata aacaacgact
     4141 tcaaaaatga tgaatatcgc gctgatgatc aacgccggga tcatcccgcc cccaaaataa
     4201 tacatctggc tgaagctata aaaaccaagc gtcaccagat tgatgg
//
LOCUS       DAOQFT010000166         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11254_length_4245_cov_7.599761, whole genome shotgun sequence.
ACCESSION   DAOQFT010000166 DAOQFT010000000
VERSION     DAOQFT010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11254_length_4245_cov_7.599761"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..446
                     /gene="secG"
                     /locus_tag="PLR93_05515"
     CDS             222..446
                     /gene="secG"
                     /locus_tag="PLR93_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNH26456.1"
                     /translation="MSNILDIALIITSVGLILSVILQSKGAGLGGLTGADTGGVFTAR
                     RGIERILFWVTIGLSVVFFALVLTIIFLGK"
     gene            534..2225
                     /locus_tag="PLR93_05520"
     CDS             534..2225
                     /locus_tag="PLR93_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH26457.1"
                     /translation="MKRLRWQILVVAVTLVIVALLLLSQQPVSVVTLPQAAPGGIYTE
                     ALIGSMGRLNPMLDWNNSADRDVNRLIFSGLIKFDSRGLPRPDLIDSWAASADGKVYN
                     FTLRKNAVWHDGQPVTSDDVLYTIEVIKSSASLFPQDIKDLWSQIEVKRLDDKTFQFI
                     LPEPFSPFLDYVTFGILPKHILESIPADQLPTAEFNLKPIGTGPYKFDRLLISNGQIT
                     GVVLVANPDYYLGAPFIEQVVFRYFPNSATALDAYRQGEVLGISQITQDVLDAALQEP
                     GLSIYTSRLPQMGLVFLNTNNPAVGFMQDAKVRRALMLGLNRTVIVSHILKGQAIVAD
                     SPILPGSWAYYDEIEKFPYDPETAAQILNKEGYVFTAGNSIRSKDGQSLSFTLVHPDD
                     AIHTQIAQAIQGDWALIGVEVKLQSVPYDSLVNEYLNSRNYEAALADLNTSRTPDPDP
                     YLFWHQAEATGGQNYSQWDDRTASEFLETARTTADFNERIRLYNNFQVVFAKELPSLP
                     LYYPVYSYGVDSQVQGVQVAPMYDVSDRLSLINEWYLVTRRTLEEQSTAEPAATP"
     gene            2288..3658
                     /locus_tag="PLR93_05525"
     CDS             2288..3658
                     /locus_tag="PLR93_05525"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNH26458.1"
                     /translation="MTDSQKAVEYVRSNSERFLNELKDLVAIPSISTLDENKSDVQRA
                     AEWVAGQLRSIGMDNVKIMPTALHPVVYGEWMGAGKSAPTVMIYGHYDVQPVDPLELW
                     TSDPFKAEVRGDYLFARGASDMKGQVVASIKAVEAIMKTEGKMPVNIKWLVEGEEEIG
                     SEHLGDFIKNNKELLAADFCLNPDAGMIGPDKPTITTGLRGLAYFELRVFGPDKDLHS
                     GLFGGTVHNPAQALIELIAGMHDKNGKITLPGFYDKVRKLSKKEREDFKRLPTTNKDL
                     IKMTGVPALWGEPQFIPAERVGARPTLEVNGLLSGFTGQGSKTVLPAWAMAKISCRLV
                     PDQTPEQTEKQLRAYLKKHAPKTIKWELKNLHNAGSALTEVDSAGVKALAKALETVWG
                     KRPYFKREGGSIGSVVLLQKYVGADSLLTGFGLPDDNVHSPNERLHLPTWYKGIEAFT
                     HFFYNF"
     gene            3752..>4245
                     /locus_tag="PLR93_05530"
     CDS             3752..>4245
                     /locus_tag="PLR93_05530"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNH26459.1"
                     /translation="MEDRIITYGGQAVIEGVMMRGQKAFAVAMRAPDGKIVTHTEKLA
                     EVYRSGLTKVPFLRGVILLWDALGLGMRALTLSANTQTGEDEKLEGPALYLTLGLSLT
                     MGIGLFFLLPAGIGGWVEHAMAQTTSALWIGNLIEGVLRLLILIGYIWSIGFMPDIRR
                     VFQY"
BASE COUNT          976 a         1131 c         1075 g         1063 t
ORIGIN      
        1 atgtctttgt gaagtccgct gccatcagag tagcgggtgt gcatgtgcag gttgacgata
       61 acttcgtgca ttcaagtcct cttgttgtaa tagacctgac aggtctttga aatctgtcag
      121 gtctgaagtt ttactgcggg tgagaggttt ttaagctcgt ttgacgattc acttctcgta
      181 caggtataat ttgcccgcta aattctagga ggcactttcg catgtcaaac attttggata
      241 ttgctttgat tataacctca gtgggtttga tcctcagcgt catcctgcag agcaagggcg
      301 cagggttggg tggtctcacc ggcgcggaca cgggtggtgt attcaccgct cgccgcggca
      361 ttgagcgtat tctgttctgg gtgacgattg gtttgagcgt cgttttcttc gcgctcgttc
      421 taaccattat cttccttgga aaataagaat tccctgcctg aaaggcccgc cgttttcaaa
      481 tgcccgcacc ccgggcaggg cggtcctttc tttttttccc acaacaaaac attatgaaac
      541 gattacgctg gcagattctg gttgtggcgg taacgcttgt gatcgtggcg ttgctcttgc
      601 tcagccaaca gccggttagt gtagtgacct tgccccaggc ggcgcccggc ggtatttata
      661 ccgaagcgct gattgggtct atgggtcgcc tcaacccgat gctggactgg aacaactctg
      721 ccgatcgaga tgtgaaccgc ctcatcttta gcggtttgat caaattcgat tcccgcggct
      781 tgccccgccc agacctgatc gattcatggg cagcctcggc agacggcaag gtctataact
      841 tcaccttgcg caagaacgcc gtctggcacg acggtcaacc ggtgaccagt gatgatgtgc
      901 tttacaccat cgaggtcatt aagagttcgg cttcgctctt cccgcaagat atcaaagact
      961 tgtggtcgca gatcgaagta aaacggctcg acgataaaac cttccaattc attctccccg
     1021 aaccgttctc tcccttttta gattatgtca cctttggcat tttgccaaag catattttgg
     1081 aatccatccc cgccgaccaa ctcccaaccg cagagtttaa cctcaagcca atcggtaccg
     1141 gtccttataa gtttgaccgg ctcctgatca gcaacgggca gatcaccggc gtggtgttgg
     1201 ttgccaaccc cgattactat ctcggcgctc cgttcattga gcaagtggtc ttccgttatt
     1261 tccccaactc tgccaccgcc ttagacgcct accgtcaggg tgaagtattg ggcattagcc
     1321 aaatcacaca agatgtcttg gatgccgcct tgcaagaacc ggggctttcc atttacacca
     1381 gccgcctccc gcaaatggga ttggtcttcc tcaacaccaa caaccctgcc gtgggcttta
     1441 tgcaagatgc aaaagtccgc cgcgcgttga tgttgggact gaatcgcacc gtgattgtct
     1501 cgcacatcct caaaggtcaa gccattgtgg cagatagccc cattttgccg ggctcatggg
     1561 cgtattacga tgagatcgaa aaattcccat acgaccccga aacagcggct cagatcttga
     1621 ataaagaagg ctatgttttc actgcgggca atagcatccg ctcgaaagat gggcaatcgc
     1681 tttccttcac cctcgtccac ccagatgacg ccatccacac ccagattgcg caagccattc
     1741 agggcgattg ggcgttgatc ggggtggagg tcaagttaca gtctgtgcct tatgactcgc
     1801 tggtgaatga atatctcaac tcgcgcaatt acgaagccgc cctggcagac ttaaacacct
     1861 cgcgcacgcc agacccagac ccctatctct tctggcatca agccgaagcc acgggtgggc
     1921 agaactattc tcagtgggat gaccgcactg cgagcgagtt cctcgagacc gcccgcacca
     1981 ctgcagattt caatgagcgc atccgcttgt acaataactt ccaagtggtc ttcgccaagg
     2041 aattgccgtc tttgcctttg tattacccgg tctattccta tggcgtagat tcgcaagtgc
     2101 aaggtgtaca agttgcacca atgtatgatg tcagtgacag gttgtctctg atcaacgaat
     2161 ggtatttggt cacacgccgt acgctcgaag agcaatccac ggctgagcct gccgcaacac
     2221 cgtaacccaa cagaccctaa aggcattcaa agcctttagg gtcattcata ctataaggag
     2281 tttcatcatg actgactctc agaaagctgt tgaatacgtc cgctcgaata gtgaaagatt
     2341 tttgaacgag ttgaaggatt tggtcgccat tccttcgatc tctactttgg atgagaacaa
     2401 atcagatgtg cagcgcgccg ccgagtgggt ggcgggtcaa ctgcgctcca tcggcatgga
     2461 taacgtgaag atcatgccga ccgcgttgca tcccgtcgtc tatggcgaat ggatgggggc
     2521 gggcaaatcg gctcccaccg tgatgatcta cggacattat gacgtccaac ccgtcgaccc
     2581 gctagagctt tggacgtctg acccgttcaa agcggaggtg cgaggcgatt atttattcgc
     2641 acgcggcgct tcggatatga agggtcaagt tgtggcatcc atcaaagcgg tggaagccat
     2701 catgaaaacc gaaggcaaaa tgccggtgaa catcaaatgg ttggtggaag gcgaagaaga
     2761 aattggctcc gaacatcttg gcgatttcat caagaacaat aaagaactgc ttgccgctga
     2821 tttttgtttg aaccccgatg ccggtatgat cggacccgat aagcccacca tcacaaccgg
     2881 tttgcgcggt ctcgcttatt ttgaattacg cgtctttggt cccgataaag atttgcactc
     2941 cggcttgttt ggcggcaccg tccacaaccc ggctcaagcg ttgatcgaac tcattgccgg
     3001 catgcacgat aagaatggca aaatcacgtt gccaggcttt tacgacaagg tccgcaagtt
     3061 gagcaagaaa gaacgcgaag atttcaaacg cctgcccacc accaacaaag acctcatcaa
     3121 aatgaccggc gtgcctgccc tgtggggaga acctcaattc atccctgccg aacgcgttgg
     3181 cgctcgccct actcttgaag tgaacggctt gctctctggt tttaccggtc agggttccaa
     3241 gaccgttttg cctgcctggg caatggcgaa aatttcctgc cgtctcgtgc ccgaccaaac
     3301 gcctgaacaa accgagaaac aattgcgcgc ctatctcaag aaacatgcgc ccaagaccat
     3361 caaatgggaa ctgaagaatc tgcacaatgc tggctccgca ttaaccgaag ttgactctgc
     3421 tggtgtgaag gcgcttgcca aggcgctcga aaccgtgtgg ggcaaacgac cttacttcaa
     3481 gcgcgaaggc ggttctattg gttcggttgt gcttctgcaa aaatacgtcg gtgccgattc
     3541 gcttctcacc ggtttcggct tgcccgatga caacgtccac tcgccgaatg agcgtttgca
     3601 tttacccact tggtataagg gcattgaagc ctttacccat ttcttttaca acttctaaat
     3661 aacaagcaag ggcgacccgt ctggcgagat caatcacttt gatccacatg ggcgggtcgc
     3721 tcttgtatat cgaaagaaag aaacgagaat tatggaagat cgaattatta catacggcgg
     3781 tcaagccgtg atcgaagggg tgatgatgcg cgggcaaaaa gctttcgccg ttgccatgcg
     3841 cgcgcccgat ggcaagatcg tcacacacac tgaaaagctg gcagaagttt atcgctctgg
     3901 tcttaccaaa gtcccttttt tgcgcggtgt gattttgtta tgggatgcgc tcggactcgg
     3961 tatgcgtgct ttgactcttt ctgccaatac tcaaactggc gaggatgaaa agctggaagg
     4021 accggctctt tatctgacct tgggcttgtc tctcaccatg ggcattggac tcttcttttt
     4081 gcttcccgcc ggaatcggcg gctgggtgga gcatgccatg gcgcagacca cctccgcgct
     4141 ttggatcggg aacctgatcg aaggcgtgtt acgtttgttg atcttgatcg ggtatatctg
     4201 gagcattggt ttcatgcccg atattcgccg tgtctttcaa tatca
//
LOCUS       DAOQFT010000167         4243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11263_length_4243_cov_8.099093, whole genome shotgun sequence.
ACCESSION   DAOQFT010000167 DAOQFT010000000
VERSION     DAOQFT010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11263_length_4243_cov_8.099093"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /gene="glgP"
                     /locus_tag="PLR93_05535"
     CDS             complement(<1..1613)
                     /gene="glgP"
                     /locus_tag="PLR93_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNH26460.1"
                     /translation="MEFLTSPSQKFDLPKRIERLGELATNLWWSWHPEAAHLFGRLDY
                     DLWERLGHNPIRILNEVGRTRLNQASKEKEYLELYDAVFKTFDAYFSKETWSQKTHKE
                     LKKPIAYFSMEYGLHETLPIYSGGLGVLSGDHLKEASDLGLPFIAVGFMYAQGYFSQR
                     ITEDGWQEALNNPLNFDHLPVSLVTKDGKAVTVSFDFPDRKLTAQIWEVRVGRVPLYL
                     LDSNVESNSEYDRLLTARLYWSDLDRRIMQEVLLGIGGARAIRALGYDPAVWHMNEGH
                     ASFLTLERIREHVASGSTFTAAIDKTRGQNIFTTHTPVPAGNDEFPLYLIDKYLSTYW
                     SELKLTREEFIDVAKHNHPQGDMYSMPVLALKLSGGSNGVSELHGEVSRDMWQFLWND
                     RSIKDVPIQHVTNGVHTRNWMARRMNNLLEKHLGADWYDNLDDHALLEKIDQIPDNEL
                     WSVHLHLKRKLSFYLKERVRDRWTRGGFHPVQVVASGVLINPYALTIGFARRFATYKR
                     ASLVLSDVERLLELINRPNMPVQIIFAGKA"
     gene            complement(1760..2107)
                     /locus_tag="PLR93_05540"
     CDS             complement(1760..2107)
                     /locus_tag="PLR93_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26461.1"
                     /translation="MPTKTPSIISTVLTVLSLLVFVIASLFFSLVALNGVSESQGTPA
                     LIASLICNGIGIILAAVVAWRMPAWLIHKFSWKSLWAVLVSLIVSFFLGGGISIVAMF
                     VSILVADILWNAR"
     gene            complement(2127..3053)
                     /locus_tag="PLR93_05545"
     CDS             complement(2127..3053)
                     /locus_tag="PLR93_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26462.1"
                     /translation="MPLRFPVTSLVLIWMLVGCSSLPFVATPTPTALPTSTPTRTPLP
                     TQTPSPTVTPILVTYPFKHVWFKYTVERPAEEANPLGLTEEPLLVIYTDGTMIAARTG
                     DPKVRTRVLNTKEVCDFLNRLDTFGFYTLEDSHSTDETNPLYDFGTKYQAVDEGDFAS
                     LTVNRTDPKSVTFFEPYRKFFARPMRKIMEFVETYNPGALKAYEPDRLVVFVQRGRAE
                     GTDEKVRVRDWTVNTFSLNDAANAPYAFLEGQAAMDVYALLAKYETHIYLENEIEYTV
                     TMRLLFPHEVLNADSTPIAIPTEARLPVNCTP"
     gene            complement(3128..3901)
                     /locus_tag="PLR93_05550"
     CDS             complement(3128..3901)
                     /locus_tag="PLR93_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNH26463.1"
                     /translation="MKLYKPTLVLVLAALLVACAPATEEASHASTPHWTYEGEEGPEH
                     WGDLDPSYAACETGTKQSPIDVSNPAEQDLANIAFHYQPSEVNILNNGHTVQVNYDAG
                     SYIELDGVRYDVAQFHYHAPSEHAVDGKLFAAELHIVHKNADGGLAVVGILLDEGAEN
                     AAYQPFIQNLPAEESDVKDAGVTVNAADLLPAVQTTFRYSGSLTTPPCTEGVNWLLMT
                     TPVEISASQIEALASLFEAGNNRPVQPLNDRPLAEDSTP"
BASE COUNT         1056 a          982 c         1132 g         1073 t
ORIGIN      
        1 gccttgcccg cgaagatgat ctgcaccggc atgttcgggc ggttgatcaa ctccagcaag
       61 cgttctacat ccgaaaggac gaggctggca cgtttgtagg tcgcgaaacg acgggcaaag
      121 ccgatagtca acgcataggg gttgataagc acacccgaag cgacaacctg aaccgggtga
      181 aacccgccgc gagtccagcg gtcgcggacg cgttctttta gatagaaaga gagcttgcgt
      241 ttgaggtgaa ggtgaacgct ccagagttca ttatctggaa tctggtcgat cttttctagc
      301 aaggcgtggt cgtcgagatt gtcgtaccaa tctgcgccaa gatgtttttc gagcaggttg
      361 ttcatgcggc gtgccatcca attgcgggtg tgaacaccgt tggtgacgtg ttgaatgggc
      421 acatctttta tactgcggtc attccaaagg aattgccaca tatcgcgcga gacttcgcca
      481 tgtaattcag aaacgccgtt cgagccgcca gagagtttga gcgccaagac gggcatggaa
      541 tacatgtcac cttgtgggtg gttgtgcttg gcaacgtcaa tgaactcttc gcgggtgagc
      601 ttcagttcgc tccagtaggt ggagaggtat ttatcaatga gatagagcgg gaattcgtcg
      661 ttgccagcgg ggacgggagt gtgagtggtg aagatgttct gtccgcgggt tttatcgatg
      721 gcggcggtaa aggtggagcc tgacgccacg tgttcacgaa tccgttcgag ggttaagaaa
      781 gaggcgtgac cttcattcat gtgccagacg gcggggtcat atcccaaggc gcggatggcg
      841 cgcgcaccgc cgattcccaa cagaacttcc tgcatgatgc ggcgatctag gtcagaccag
      901 tagaggcggg cggtcagcag acggtcatat tcgctgttgc tttcgacgtt tgagtcgagc
      961 aagtagagcg gaacacgccc aacacgcact tcccaaattt gcgcggtgag ctttcgatca
     1021 ggaaaatcga atgaaacggt gacggctttg ccatctttgg tgacaaggga gacgggcagg
     1081 tgatcgaagt tgagcgggtt gttgagagct tcctgccagc cgtcttcagt gatgcgttgg
     1141 gagaaatatc cctgagcata catgaagccg actgcgatga aaggcaagcc gaggtctgag
     1201 gcttctttga gatgatctcc tgaaagcacg cccaagccgc cagaataaat gggcagggtt
     1261 tcatgcaagc catattccat cgagaaatag gcaatcggtt ttttcaactc tttgtgcgtc
     1321 ttttgtgacc aggtttcttt gctaaagtat gcatcgaaag ttttgaagac ggcatcatat
     1381 agttcgagat attctttttc tttcgatgcc tgatttaagc gtgtgcgccc aacttcatta
     1441 agaatgcgga tggggttgtg cccgagtcgt tcccacaggt cataatccaa acgaccaaag
     1501 agatgcgcag cttcaggatg ccaggaccac cacaagttgg tggcaagttc accaagacgt
     1561 tcgatacgtt ttggcaaatc gaatttttga gagggagaag ttaagaattc catagtgggc
     1621 ggatgatacc tcattgtttt tttgcgtcca gcgattgtaa ggcggattag ttgtttggtc
     1681 aaactaaatg aacttcggaa ttaaaaatga cggtcacctg taaagatgac cgtcatttga
     1741 gcaaaatgtt gattcaggtt taacgggcat tccataatat gtccgctacg agaatgctga
     1801 cgaacatggc aacgatagaa atccctccgc caaggaagaa gctgactatt aaggaaacca
     1861 gcactgccca aaggcttttc cagctaaatt tatggatcag ccaggcaggc attctccatg
     1921 cgacgacggc ggcgaggatg attccgatcc cattacatat cagcgatgcg atcagcgcgg
     1981 gcgtgccctg actttctgaa accccattca gagcaaccaa agaaaagaac aagctggcga
     2041 tcacaaacac aagcaagcta agaaccgtta gcacagtaga aataatggac ggggttttgg
     2101 taggcatggg tttcctcaaa taaaggttat ggtgtgcaat tcacaggaag gcgcgcttcg
     2161 gtggggatgg caatcggcgt agagtctgca ttaagcactt catgtgggaa cagcaaacgc
     2221 atcgtgactg tgtattctat ttcattctca agatagatgt gggtttcata cttcgccagc
     2281 aatgcataca catccatggc agcttgccct tctagaaatg catagggcgc attggcagca
     2341 tcgttcaggc tgaaagtgtt caccgtccaa tcacgtacgc ggactttttc atcggtgcct
     2401 tcggctcgtc cgcgttgcac gaaaacgacc aaacggtctg gctcatatgc cttgagcgcg
     2461 ccaggattgt acgtttctac aaattccatg atcttgcgca tgggtcttgc aaaaaacttt
     2521 cgataaggct caaagaaagt tacgcttttg gggtccgtcc gattcacagt gaggctggca
     2581 aaatcacctt catccacggc ttgatatttt gtgccaaaat catataaagg atttgtctca
     2641 tctgtcgagt gtgaatcttc cagcgtgtaa aaaccgaagg tatctagccg gttgagaaag
     2701 tcgcagactt ctttcgtgtt gagaacccgc gtgcggactt tcggatcccc cgtccgcgct
     2761 gcgatcatgg tgccatcggt atagatgacg agcagcggct cttccgtcaa cccaagcgga
     2821 ttcgcctcct cagccgggcg ctccactgtg tacttaaacc acacatgctt gaacggatac
     2881 gtgacgagaa taggtgtaac cgtaggcgaa ggcgtctgcg tagggagcgg agtccgcgtt
     2941 ggggtagagg tgggaagcgc tgtaggtgtg ggcgtggcaa caaaaggcaa agaactacag
     3001 ccaaccaaca tccatatcaa aacaagcgag gtcacaggaa atcgtagagg catgaaattt
     3061 gtcttgaatt atatcccgcc cttaaaagag acagtcacct caaaggtgac tgtctcttga
     3121 attcttgtta cggtgtacta tcttccgcca gcggacggtc gttcaagggt tggacgggtc
     3181 tgttattgcc tgcttcaaat aaagaagcaa gggcttcgat ctggcttgca gagatttcca
     3241 caggtgtagt cataagcaac cagttcacac cttcagtaca aggtggtgtg gtaagcgagc
     3301 cgctataacg gaaagtcgtt tgcacagcag ggagcaaatc tgcggcgttg acggtaacgc
     3361 ctgcatcttt cacgtcactt tcttctgctg ggaggttttg aatgaacggt tgataggctg
     3421 cattttctgc gccttcgtct aataaaatac ctaccactgc taagccgcca tctgcattct
     3481 tgtggacgat atgcaattct gctgcgaaga gtttcccatc tacggcgtgt tcactgggcg
     3541 catgataatg gaattgagcc acatcatagc ggacaccatc taattcaatg taactgcccg
     3601 catcatagtt gacttggacg gtatgtccat tgttcagaat attgacttcg cttggctgat
     3661 aatgaaaagc gatattcgcc aaatcctgtt cagctgggtt ggaaacatcg atcggtgatt
     3721 gcttggtacc tgtttcgcag gctgcatacg atgggtcaag gtcgccccaa tgttcaggac
     3781 cttcttcgcc ttcatacgtc cagtgcggag tggaagcgtg tgaggcttct tctgtcgcag
     3841 gtgcgcaggc aacaagtaag gcagctagta caagtacgag tgtgggttta tataacttca
     3901 tttgattaac tctctcctat ttaatttgat gtattgtatt ggtttgtttg aaaaggttct
     3961 ttgcacctgc tttacacttc atcagacttt cattaacctc cccctttgtc cagttgcctg
     4021 attggtcgct tggtcttata gtcacagcat tccgtagggg cgtcccgtct tgctgtgaca
     4081 agtcaatttt catgcaaaga tacatgacgc gccatcacct caccaaaata ttgacgggtc
     4141 gtccctacac ctaattatcg gagataactt ttatgcacgg atacggctgg ttcatggcaa
     4201 tgcgccaagg cgacgaaaaa cccaaagtca cccgcgagct ttt
//
LOCUS       DAOQFT010000168         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11279_length_4240_cov_7.845161, whole genome shotgun sequence.
ACCESSION   DAOQFT010000168 DAOQFT010000000
VERSION     DAOQFT010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11279_length_4240_cov_7.845161"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLR93_05555"
     CDS             <1..748
                     /locus_tag="PLR93_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNH26464.1"
                     /translation="PTIEQNAEPEILWWVGCAPATDTRAQKTAQAFAKILNTAGVNYA
                     VLGKNEACTGDSARRAGREDIFFGLASQNVEILNEVAPKRIVTTCPHCLHTIKNEYPA
                     FGGNYQVIHHTQLINELVGAGKISMNLEGDNMKITFHDPCYLGRHNKVTDAPRDALKS
                     AGVETVEMPRNGAKSFCCGAGGAQMWKEEEQGTARVNATRFAEAKSTGADTVAVGCPF
                     CLTMMTDASKGDGGTIQVKDVAEIVAERLK"
     gene            781..1149
                     /locus_tag="PLR93_05560"
     CDS             781..1149
                     /locus_tag="PLR93_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26465.1"
                     /translation="MSEAKKVVIERKKGYLSVEYAKPYNIDDLLSFSKEALEVSKAEG
                     YKKLFLDISKMPGRIKPMERYEVGVKAATLFRYKLKVAVLYKTEEINRFAETVALNRG
                     MNVKIFNTKEEAISWLEVEE"
     gene            1283..3703
                     /locus_tag="PLR93_05565"
     CDS             1283..3703
                     /locus_tag="PLR93_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH26466.1"
                     /translation="MPNKKKGTIALPLEKDEIIKDYRLAYQSRQASLIGRREVLSGKA
                     KFGIFGDGKEIAQLAIARAFQKGDWRSGYYRDQTWMFMLGIMSIQEFFAQLYAHADAQ
                     YDPASGGRQMNAHFATRHLHPNGAWRAQTQLYNTSSDVSCTAAQMPRAVGLAYASVLY
                     RNIKELNNLKDFSINGNEVTFATIGNASTAEGHFWEAVNAIGVLKAPAVITVYDDGYG
                     ISVPNQFQMVKENIGTLLKGFERDPRTPHNKVQNGYDHYTVRAWDYSALIETYQTAAE
                     VAREYHIPALVHVVDVTQPQGHSTSGSHERYKTPERLKWEEEFDGLKKMREWMITNRV
                     ISAPELDALEKADYDTVEGFRKAAWEAYLAPLTEERNQVMDILEEIASSTPNHVAELR
                     MLRDRLTALPSFSRRDIHSIAHEALRLLRNESHPSKQVLIQWKHENDKLTEERYSSHL
                     YSGTALKVEEIKPVYSDSSPTMFGFEVINAAFDAALTRDSKLIAFGEDVGKLGDVNQG
                     FKGMQEKYGEFRVTDTGIREATIMGQAIGMAMRGLRPIAEIQYLDYVLYALQTMSDDL
                     ASLHWRTAGGQKAPVIVRTRGHRLEGVWHAGSPMSGILSLTRGMYVLVPRDMTRAAGF
                     YNTLLKSDEPAIVVEVLNGYRVKEKLPDNIGEITLPLGVPETIRQGKDITIVTYGACC
                     RIVLDAAEKLSKVGIDVEVIDVQSLMPFDIHGKIVESLKNTNHILFVDEDVPGGATAY
                     MMQEVIEKQGGYFHLDSPARTLSAKAHRPSYGSDGDYWSKPNAETVFDVVYEMMHEVN
                     PTAFPLFS"
     gene            3727..>4240
                     /locus_tag="PLR93_05570"
     CDS             3727..>4240
                     /locus_tag="PLR93_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNH26467.1"
                     /translation="MATKVYVPRLGEGVDEVTVTKWLKQVGDSINELEPLLEVNTDKV
                     DTEIPAPATGTLLQIVAQEGVPAKVGELLAIIGKPGETVDSGQASIESVASSPTPVTQ
                     QSPAVIPQPQSINRDLGFISPVVAKIAAEHGVDLSQVNGTGLNGRITKNDVLNFVESG
                     KSKGTGQPSNI"
BASE COUNT         1197 a          980 c         1030 g         1033 t
ORIGIN      
        1 gccgaccatt gagcaaaatg ccgaacctga aattctttgg tgggttggct gcgcaccagc
       61 caccgataca cgcgcacaga aaactgcgca agcctttgca aaaattttga acacggctgg
      121 agtgaactat gccgtgcttg gcaagaacga agcctgcacc ggtgactcgg ctcgccgtgc
      181 tggacgcgaa gatatattct ttggtttggc ttcacagaat gtagaaatct tgaacgaagt
      241 tgcgccaaaa cgcattgtga cgacttgtcc gcattgtctg cacacgatca agaatgaata
      301 ccccgccttc ggtggaaact atcaggtgat tcaccatacg caattgatca acgaactcgt
      361 gggcgcaggc aaaatctcca tgaaccttga aggcgataac atgaagatca ccttccatga
      421 cccatgctat ttagggcgcc acaacaaggt cactgatgcg ccgcgtgatg cgttgaaatc
      481 cgctggtgtt gaaacggtgg aaatgccacg caatggtgct aaatctttct gctgtggtgc
      541 aggcggcgca caaatgtgga aagaagaaga acaaggtacg gcaagggtca atgccactcg
      601 ctttgccgaa gccaaatcca ccggagccga taccgtggca gtgggctgtc ccttctgcct
      661 tacgatgatg accgatgcat ccaaaggaga tgggggcacg atccaagtga aagatgtggc
      721 agagatcgta gcggaacgat tgaaatagaa gaataaattt gaaaacgccg actcaactgt
      781 atgtcagaag cgaaaaaagt agtcattgaa agaaaaaaag gctatctgtc tgtagaatat
      841 gcaaagccct acaatataga tgaccttcta agtttttcaa aagaagcatt ggaagtttcc
      901 aaggctgaag gctataaaaa gttatttttg gatatttcca aaatgcctgg caggatcaag
      961 cctatggaac gctatgaggt tggagtaaaa gcagctacgc tttttcgata taagctcaaa
     1021 gtggcggttt tatataagac cgaagagatc aaccgctttg cagagaccgt tgcccttaac
     1081 cgcggaatga atgtaaaaat cttcaataca aaagaagaag ccatttcatg gcttgaagtt
     1141 gaagaataga aaaaacaaaa aaaagaaaaa gcggatagtt ttatccgctt tttctttttt
     1201 ttcacaatca aaatccagcg agaaaccttt cccctcttcc tttgttataa tccttgaaca
     1261 atcaactcaa caggagagat tcatgcccaa caagaagaag ggtacgattg cacttcccct
     1321 tgaaaaagat gagatcataa aagattaccg tcttgcctat caaagcaggc aggcatccct
     1381 tattggtaga cgtgaagttc ttagtggcaa agcaaaattt ggtatttttg gcgatggcaa
     1441 agagatcgct caactcgcca ttgctcgtgc ttttcaaaaa ggcgattggc gttcaggcta
     1501 ttatcgagac cagacctgga tgttcatgct cggtatcatg agcattcaag aattctttgc
     1561 gcagttatac gcacatgccg atgctcaata cgaccccgcc agtggcggca ggcaaatgaa
     1621 tgctcatttt gccacccgtc accttcaccc caatggtgct tggcgtgcac aaacccaact
     1681 ctataacact tcatccgatg tctcttgtac agccgctcaa atgcccagag ccgttgggtt
     1741 ggcgtatgca tcggtcttat atcgcaacat caaagaatta aacaatctca aagatttttc
     1801 gatcaacggc aatgaagtga cctttgccac catcggcaat gcctctacag cagaaggtca
     1861 tttttgggag gcggtcaacg cgattggggt tctcaaagcg ccagccgtta tcaccgtcta
     1921 tgatgacgga tatggcatct cggtgccaaa tcaatttcag atggtcaaag aaaatattgg
     1981 gacattgctg aaaggctttg aacgtgaccc gcgcacacca cacaacaaag tgcaaaacgg
     2041 ctatgaccat tacaccgttc gcgcttggga ttattctgca ctcattgaaa cgtatcaaac
     2101 cgctgcagag gttgcgcgcg aatatcacat ccctgcacta gtgcacgttg tggatgtcac
     2161 acagccgcag ggtcactcca cctctggcag ccatgaaaga tataagacgc ctgaacggct
     2221 caagtgggaa gaagaatttg acggcttgaa aaagatgcgt gagtggatga tcacgaaccg
     2281 cgtcatttcc gcgcccgagc tggatgccct cgaaaaagca gactacgaca ccgtagaagg
     2341 attccgcaaa gctgcatggg aagcttatct cgcaccgttg accgaagaac gcaatcaggt
     2401 gatggatatc ctcgaagaga ttgccagctc gactccgaat catgttgcag agttgcgaat
     2461 gctcagagat cggctgacag ccctcccgtc gttttcgagg cgcgatatcc attccattgc
     2521 gcatgaggca ttgcgtcttt tacgaaatga gtctcatcct tcgaaacaag ttctcattca
     2581 atggaagcat gaaaatgaca agctgaccga agagcgctat agttcgcatt tgtactctgg
     2641 cacagcactc aaagtggaag aaatcaagcc ggtttattca gatagctccc ccaccatgtt
     2701 tggttttgaa gtgatcaatg cagcctttga tgcagcactt acccgtgact caaaactaat
     2761 tgctttcggt gaagatgttg gcaagcttgg cgatgtcaat cagggtttta aggggatgca
     2821 agaaaaatac ggcgaattcc gtgtgaccga taccggtatt cgtgaagcta ccattatggg
     2881 acaagccatt ggcatggcaa tgcgcggatt acgcccgatt gctgaaattc aataccttga
     2941 ttatgtcttg tatgcgttgc aaaccatgag tgatgacctt gcttcattgc attggcgcac
     3001 cgctggtgga cagaaagcgc ctgttatcgt ccgcacacgt gggcatcgtt tggaaggtgt
     3061 ttggcatgca ggctcgccca tgtctggcat tcttagtttg actcgcggca tgtatgtttt
     3121 ggttccgcgc gatatgaccc gcgccgctgg tttctacaac accttattga aatcggatga
     3181 gccggctatt gttgttgaag ttctaaatgg gtatcgtgtc aaagagaaat tgcccgataa
     3241 cattggtgaa attacattac ccttgggtgt tcctgaaacc attcgccaag gcaaagacat
     3301 taccatcgtc acttatgggg cgtgctgtcg tattgtgttg gatgctgctg agaaattgag
     3361 caaggtagga atcgatgtgg aagtgatcga cgtccaatcg ctgatgccct tcgatattca
     3421 tggcaagatc gtggaatcgt taaagaacac caaccatatt ctcttcgtag atgaagatgt
     3481 tcccggtggg gcaactgcct atatgatgca agaagtcatc gagaagcaag gcggttattt
     3541 ccatttagac tcgcccgcac gtacattatc tgccaaagcg catcgtccat catatggctc
     3601 agacggagat tactggagca agcccaatgc tgagaccgtg tttgatgtgg tgtacgagat
     3661 gatgcatgaa gtgaatccga ctgcatttcc actttttagt tgatagcacc ccaactggag
     3721 aaattcatgg ctacaaaagt ttatgtaccc cggctcggcg aaggcgtgga cgaagttact
     3781 gttacaaaat ggctcaaaca agttggcgat tctattaatg aattggaacc gctgcttgaa
     3841 gtgaacacag acaaagttga cacagaaatc ccagcccctg ctacgggaac tctcttgcag
     3901 atcgttgcac aggaaggtgt gcccgcaaag gtaggcgaac tgttagccat cattggtaaa
     3961 ccaggggaaa ctgtggatag tggacaagcg tccatagaaa gtgttgcttc ttcgccaaca
     4021 cctgttactc aacaatctcc tgctgtcatt ccacaacctc aatcaatcaa ccgcgatctt
     4081 ggcttcatct ctcctgttgt ggcaaaaatc gccgctgaac acggcgtgga cttgtctcag
     4141 gtcaacggca cggggctgaa cggacgcatt acgaaaaacg atgtgttgaa tttcgtcgaa
     4201 agtggaaagt caaaagggac aggtcaacct tccaacattt
//
LOCUS       DAOQFT010000169         4239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11285_length_4239_cov_7.063337, whole genome shotgun sequence.
ACCESSION   DAOQFT010000169 DAOQFT010000000
VERSION     DAOQFT010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11285_length_4239_cov_7.063337"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLR93_05575"
     CDS             <1..890
                     /locus_tag="PLR93_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26468.1"
                     /translation="QQAFAREDFYNKIPNLMAQSIVSGADMGQLPTVTQGMNLETWEN
                     FIRILLPPEVLKPIGDDVLISTFAYLNMERNSVQVNLTPVRASMMSESGSQAILFLLN
                     GLPACSVEQIAQITFDLLSGGQIQLCNPPADMLPVLMPVIQGEMQATAAVIPDQLTLF
                     TAPFQDDPREKLQTARLLMRLSLILPIGCLLALTLFAVRSLQDWLTWWGVPLAATGFS
                     SFIIGLIGAPILGAVLESILSRKLPDYLPSFLLDFTSDFAAAMVRALLTPVAWQGLLI
                     SFLGACMAGVGYYLKYKQP"
     gene            932..2047
                     /locus_tag="PLR93_05580"
     CDS             932..2047
                     /locus_tag="PLR93_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26469.1"
                     /translation="MRRIFIFLSFLLVLSLACGGSPTPPPPLETSIFDSSETAYGFYP
                     SPPEVTLESVMQTFKAMGEHGDFVLIQQNVDWESFAKGVDGESQLRTDIINQVTLSRQ
                     NNLDTIFVLDALNGLNRREFFNLPSTWEASFGNPEVRTAFKNYALWVMRNFQPHYLGL
                     ASEINTYMDAHPDDAQNFISLYDEIYGLVKAEAPDTQIFVTFQWDDLNNMFAQPEEGD
                     AQRLSPKWEQIEAFEPNLDVWAISSYPYFVFPAGADIPSDYYAPLLSRTSKPVALAEG
                     GFSTVAFNELNNTPEDQVAYLNAIHEQLGPRMAFWVNTLLSDFNIVSYAKEMEKQGRN
                     PDDAQMLGNFAYIGLREFDGTPKPALKVWDGFRNSTP"
     gene            complement(2044..2478)
                     /locus_tag="PLR93_05585"
     CDS             complement(2044..2478)
                     /locus_tag="PLR93_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26470.1"
                     /translation="MNIDDLKMRLEEEACSRANYSIGERDSDVFCLENQNGIWRIFYT
                     ERGIDQPPIFESPNESEACEYFFTFMTTKIRHDHIVGYFISREKAEALVETLAQHNIQ
                     TYRNDIPYHGWHDPRFRVFVIGKDVFKAREILGELPVRDIRD"
     gene            complement(2475..3641)
                     /gene="mltG"
                     /locus_tag="PLR93_05590"
     CDS             complement(2475..3641)
                     /gene="mltG"
                     /locus_tag="PLR93_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNH26471.1"
                     /translation="MNDTQPQAPVRRRKPRNSMRRYQLYFALGSIVLAAFICIFVFIY
                     YVPAQATLLYGPPAGHLSISDRIEYSTRLLSHGTELTTPLNVNGLEQSFRVEPGESVA
                     SIANRLEGLGIISSADAFFDYAVYTGIDLTIQSGDFNLSPAQSIVDIAQSLQKFNPSD
                     AVLSILPGWRIEEIAASLPTSGLSIAPEAFLAAAQTPPQVLAFAAPTTMEGFFFPDTY
                     TLPRTTTVDQLLDVVARNFTTKLTTDIQNGFAAQNLSVYQGVILASIVQREAVKIEEA
                     PVIASVYLNRISIGMKLDADPTVQYALGYQFDNGSWWKSPLALTDLEVISPYNTYLNN
                     SLPPTPISSPGLEALMAVAYPQSTPYYYFQAACDGSGYHVFVVTYEEQIANGCQ"
     gene            complement(3638..4057)
                     /gene="ruvX"
                     /locus_tag="PLR93_05595"
     CDS             complement(3638..4057)
                     /gene="ruvX"
                     /locus_tag="PLR93_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNH26472.1"
                     /translation="MRILAVDHGEKRIGLALSDPTATIASPLKVIQHVSRLIDAAQVA
                     EIAMQNEVGLIVVGQSYDEEGNPNPAGRRAGRFADELRNQTNIPIEMWDESHSTQIAR
                     AARIELGVSRKKRAGHQDEFAAVVILQSYLEAKRNSE"
BASE COUNT         1080 a         1046 c         1013 g         1100 t
ORIGIN      
        1 atcaacaagc gtttgcacgt gaagactttt acaacaaaat ccccaacctc atggcgcaat
       61 ccatcgtatc cggtgcagac atggggcagc taccaacggt cacacagggc atgaaccttg
      121 agacctggga gaatttcatt cgcattctgt tgccacccga agttttgaaa ccaatcggcg
      181 atgatgtatt gatctcaacc tttgcgtatc tcaacatgga gcgcaattcg gtgcaagtga
      241 accttacgcc ggtccgcgcc agcatgatga gcgagagcgg ctcacaagcc attttgtttt
      301 tactcaatgg cttgcctgca tgcagtgtag aacaaattgc gcaaatcacc tttgacctgc
      361 tttcaggcgg gcagatccaa ctctgcaatc caccggcaga catgttgccc gtgctcatgc
      421 ccgtcattca gggagaaatg caagccactg ccgccgtcat cccagatcaa ttgactttgt
      481 ttaccgcgcc ctttcaagat gacccacgag aaaaactgca aaccgcccgg ctcttaatgc
      541 gcctgagctt gatcctcccc atcggctgcc tactcgcgtt gactctgttc gccgtccgtt
      601 ccctgcaaga ttggctgaca tggtggggcg ttccgcttgc agccactggc ttcagcagtt
      661 ttataattgg actgatcggt gctcccattt taggggctgt gcttgaaagt atcctttcaa
      721 ggaaattacc agactatctc cccagctttt tactggattt cactagcgac tttgccgcag
      781 ccatggtgcg agccttgctt acgcccgttg catggcaagg cttgttaatc agttttcttg
      841 gcgcttgtat ggcaggggtt ggatactacc taaaatataa acagccttaa tcacttgcaa
      901 cttttcgccg catcttcttg tattacaagt tatgcggcga atttttattt tcctatcctt
      961 tcttctcgtt ctatctttgg cttgcggtgg ttctcctact ccacctcccc cacttgaaac
     1021 ctctatcttt gattccagtg aaacggctta tggcttttat ccttctccac cagaagtgac
     1081 ccttgaaagc gttatgcaaa ccttcaaggc aatgggagaa catggcgatt ttgtcctgat
     1141 ccaacaaaat gtagattggg aaagtttcgc aaaaggcgta gatggagagt ctcaactgcg
     1201 aaccgacatc atcaaccaag tgaccctttc cagacaaaac aatctcgata caatcttcgt
     1261 cctcgatgct ttgaatggat taaatcggcg cgaattcttc aacttgccca gcacttggga
     1321 agccagcttt ggcaacccgg aggtacgcac cgcctttaag aactatgcct tgtgggtgat
     1381 gcgtaatttt caaccgcatt atcttgggct ggcttctgaa atcaacacct atatggatgc
     1441 tcatccagat gatgcgcaga acttcatcag cctatacgac gaaatctatg ggctcgtcaa
     1501 agctgaggca cctgatacgc aaatcttcgt cacatttcaa tgggatgact tgaacaacat
     1561 gttcgcacaa cccgaagagg gcgatgcgca acgcctttcg ccaaagtggg aacagatcga
     1621 agcttttgaa ccgaatttgg acgtatgggc gatctccagt tacccttact tcgtcttccc
     1681 cgccggtgca gatattccct ctgattacta tgccccgctt ctctcgcgga cctcgaaacc
     1741 agttgccctt gccgaaggcg gattctccac ggttgctttc aacgaactaa acaacacccc
     1801 tgaagaccag gttgcatacc tgaatgctat tcacgagcag ttaggtccac gcatggcgtt
     1861 ttgggtgaac accttgttaa gtgacttcaa catagtttct tatgcaaaag aaatggaaaa
     1921 acaagggcgc aatccagacg atgcccaaat gcttggaaac ttcgcttata tcggcttacg
     1981 tgaatttgat ggcacaccca aacctgcatt gaaagtttgg gatggtttta gaaattccac
     2041 accttaatct cgaatatcac gaacaggcaa ttcgcctaaa atctcgcggg ctttgaacac
     2101 atctttgcca atcacaaata ctcggaaacg cgggtcatgc cagccatggt agggaatgtc
     2161 atttcggtag gtttggatat tatgttgagc caatgtctct accaaggctt cggctttttc
     2221 acgagagata aaataaccca caatatggtc atgccgaatt ttggtggtca tgaaggtgaa
     2281 gaaatattca caagcttctg attcgtttgg gctttcaaag atcggaggct ggtctatacc
     2341 gcgttcggta taaaaaatcc gccagattcc attttgattt tcaaggcaaa agacatctga
     2401 gtcgcgctca ccaatcgaat aattggcgcg gctacaggct tcttcttcca aacgcatttt
     2461 caggtcgtca atgttcattg acatccattc gcgatctgct cttcataggt tactacaaag
     2521 acgtgatacc cagagccatc gcacgccgct tgaaaataat aatacggcgt agactgtggg
     2581 tatgccaccg ccatcaacgc ctcaagaccg gggcttgaga tgggcgtggg cggcaagcta
     2641 ttgtttagat aggtgttata tggcgagatg acttccaggt cggtcaatgc cagcggactc
     2701 ttccaccaag agccgttatc aaattgatat cccaatgcat attgaactgt aggatctgca
     2761 tctagtttca taccgatgga aatgcgattg agatagacag aagcaatgac cggcgcttct
     2821 tcgattttta cagcttctcg ttgaacaata gaggcaagga tcaccccttg ataaacgctg
     2881 agattctgcg cagcaaaacc gttttgaata tcggttgtca atttagtggt gaagttacgc
     2941 gcaaccacat cgagcaattg gtctactgtg gttgtgcgtg gaagggtata tgtatctggg
     3001 aagaaaaatc cttccatggt ggtgggagcg gcaaaggcaa gcacttgagg cggagtctgt
     3061 gcggcggcga gaaaagcttc gggtgcaatg gacaagcccg aggtcgggag tgaggcggcg
     3121 atttcttcga tccgccaacc gggcaagatc gagaggacgg catcgctggg attgaatttt
     3181 tggagcgact gagcaatgtc tacaatcgat tgtgccgggc tgaggttgaa atcaccagat
     3241 tgaatggtga ggtcaatgcc ggtgtaaaca gcgtaatcaa aaaacgcatc ggcacttgag
     3301 ataatgccca acccctcaag ccgattcgca atggacgcca cggactcgcc cggctctacc
     3361 cggaatgatt gttcgagccc attcacattc aaaggggttg tgagttcagt accgtgcgaa
     3421 agtaagcgag ttgaatattc gatacggtct gaaatggaaa gatgcccggc aggtggacca
     3481 tacaaaagtg tggcttgcgc agggacataa tagataaaca caaagataca tataaaagca
     3541 gccaacacaa tcgaccctaa agcaaaatag agttgatagc ggcgcatgga gtttctaggt
     3601 tttcgtcttc gaactggggc ttgtggttga gtatcgttca ttcggaatta cgtttcgctt
     3661 ctaaataaga ttgtaagatg accactgcgg cgaactcatc ttgatgcccg gcacgtttct
     3721 tgcgtgaaac gccaagttca atacgagcgg cgcgggcgat ctgggtgctg tgagattcat
     3781 cccacatttc gatgggaata ttggtttgat tacgaagctc atcggcaaag cgcccggctc
     3841 tgcgcccggc tgggttgggg ttgccctctt catcatacga ctgcccgaca acgattagac
     3901 ccacttcatt ttgcatggca atctctgcca cctgagctgc atcgattaag cgtgagacat
     3961 gttgaatcac tttcaaaggg ctggcaatcg tcgcggttgg gtcgctgaga gcaagaccga
     4021 ttcgtttttc accgtggtca acggcaagga ttctcatact attgtctact gtactttcac
     4081 tgaaatattg aaagggatca acggcagcca cttccaccaa gaaggtgtca tggtgatctg
     4141 gggttcgttt tgcaacgcca acgtatcttt cactaacaac gtcgcttgtt ggacgttccg
     4201 tccgcgaatg aggtctaaaa cctgcatcac attcacatc
//
LOCUS       DAOQFT010000170         4225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11333_length_4225_cov_6.941007, whole genome shotgun sequence.
ACCESSION   DAOQFT010000170 DAOQFT010000000
VERSION     DAOQFT010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11333_length_4225_cov_6.941007"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLR93_05600"
     CDS             complement(<1..695)
                     /locus_tag="PLR93_05600"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNH26473.1"
                     /translation="MADKLTIGVLTSGGDAPGMNAAIRAVVRTALANNMNVIGVMHGY
                     EGLVNGEFKPMGARDVGGILQRGGTVLLTSRSKRFQEPTGQREAIRKMNEAGMDALIV
                     IGGEGSMNGANALAQQGVKVIGIPGSIDNDIWGTNIAIGTDTAMNTIMDAVDKLRDTA
                     SSHQRAFLIETMGRDSGYLAVMAGIICGAEMVLIPEHPVEQQEVANAVEDAYKRGKTH
                     AIIINAEGSGIRTT"
     gene            complement(697..1617)
                     /locus_tag="PLR93_05605"
     CDS             complement(697..1617)
                     /locus_tag="PLR93_05605"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNH26474.1"
                     /translation="MFEILFLGTSASAPSAHRGLSSHIVSHNEYRFLVDCGEGTQRQI
                     LQSGKGFKRLTRALITHGHLDHILGLGGLLSTFLRWEAIEEFEVYGSKGTLERVKTLL
                     FDVVLRGKKTPMPLRLIEIKPGVIFDAEDFTVTAFSVSHGKADSLGYIFEEKARRPFL
                     AEKADALGVPFGPERKALVNGEAITLADGTHISPEEVLGDWEKGSKLVVVGDAGRTDN
                     LIEACQDADAVVIESTYLDEEADMASQFSHLTAKMGAELAQKAGVKKLILTHISRRYR
                     GRDVLAEAQTVFPNTVVARDFDTFVIKKDN"
     gene            1893..>4225
                     /locus_tag="PLR93_05610"
     CDS             1893..>4225
                     /locus_tag="PLR93_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNH26475.1"
                     /translation="MPLLKPSAPQSNSKKPAGKTLPAKKGGADLPSKAPAKGRGLPPQ
                     APPSPPPSYTPQPLREQTWWEKLSPERKLDVVGVILAFFGLVILLGLLSANRSAIVGG
                     AIFFLAQLFGWGVYVLPIGLLVFGLWLVFRKIERIPPLSTERAVGSVILFFWLMTLLH
                     SFIATAETAEAVALTGQGGGYFGGLAQRILWGAFGAGGAFIAMIAWLIIGVAVLLDKP
                     VIELFFWLKPLMLKLRDLIIKPAPQTQPVTAQPQSSDGLTPIDSTAMPAMDMTNAVPV
                     QPVQTRSGTTIIEWKLPDLTDILDEGSEATVNEDFIKQRAHLIEETLASFGAPAQVVA
                     ISRGPSITQFGVEPLFLESRGGVRTRVRVSKIASLADDLALALAAPRIRIQAPVPGHS
                     YVGIEVPNDEMARVALRDILEGEIFKGYKKPLAFALGRDVAGLPAISSIENMPHLLIA
                     GTTGSGKSVCVNSILTCMLLYNTPDDLRLVLVDPKRVELTGYNGVPHLLGPVVVEMDR
                     VIGALQWITREMDKRYHMFAQVGSRNITDYNAKMKLKGEKKLPYLVIVIDELADLMMI
                     APDETEKTITRLAQLARATGIHMILATQRPSVDVVTGLIKANFPARIAFAVASNTDSR
                     VILDQPGAEKLLGRGDMLYQAPDAPAAARLQGVFVSDNEIHRLVEYWQEQAGTGKPQP
                     SDADVPANTIPENVPLKQVPLWEENPKVSGDPLFDEAVAIVRKEGKGSVSMLQRRLRI
                     GYTRASRIMDTMEDKKIVGPPEGATQMRQVLNYGDVQP"
BASE COUNT         1070 a         1150 c          964 g         1041 t
ORIGIN      
        1 gttgtgcgaa tgcctgaccc ctcagcattg ataatgatcg catgagtctt tccacgctta
       61 taagcatctt caactgcatt tgccacttcc tgctgttcta cagggtgttc agggatcaaa
      121 accatttcag cgccacagat aatacctgcc atcacagcca ggtaaccgct atcacgcccc
      181 atggtttcga tcaaaaaggc acgctgatgg gatgaagccg tatcgcgcaa tttatccaca
      241 gcatccataa tcgtgttcat ggctgtatcg gtaccaatgg caatgtttgt accccaaata
      301 tcgttatcaa tgctaccggg gatgccgatg actttcacac cttgttgagc aagggcattc
      361 gcgccattca tagagccttc accaccaata acgatcaatg catccatgcc ggcttcattc
      421 atcttgcgga tcgcctcgcg ctgaccagtg ggttcctgga agcgtttgct gcgactggta
      481 agaagcaccg ttccaccgcg ttgcaagata ccacctacat cacgggcacc catcggtttg
      541 aactcgccgt tgactaatcc ttcataacca tgcatcacac cgattacgtt catattgttt
      601 gctaacgccg tacgcacaac tgcgcggatc gccgcattca tgccgggggc atcgcctccg
      661 gaagtcaaaa caccaattgt aagtttatct gccatctcaa ttatctttct ttatcacaaa
      721 agtatcaaaa tcacgagcca ctactgtatt tggaaaaaca gtctgtgctt ctgcaagcac
      781 atcccttcca cgatagcgtc gtgaaatatg cgtaagtatt agttttttaa cccctgcttt
      841 ttgtgcgagt tccgcaccca ttttcgcggt caaatgcgaa aactgacttg ccatatccgc
      901 ttcttcatcc agataggtag actcaatcac aactgcatcg gcatcctgac aagcttcgat
      961 caagttatca gttctacctg catcacccac aacaaccagt ttactgcctt tttcccaatc
     1021 cccaagcact tcttctggag agatatgtgt tccatcagca agtgtaatcg cctcgccatt
     1081 caccaaagct tttcgctcag gtccgaacgg cacacctaga gcatctgctt tttctgcaag
     1141 gaaaggacgg cgtgcttttt cttcaaagat atagcctagg ctatctgcct tgccatggga
     1201 aacagaaaag gcggttacag tgaaatcttc tgcatcgaag atcacgccgg gttttatctc
     1261 gatcaaacgc aaaggcatgg gcgttttctt gccgcgcaac accacatcga acaacaaagt
     1321 ctttacacgt tcaagcgttc ccttactgcc atacacttca aactcttcaa tcgcttccca
     1381 acgcaaaaaa gtggaaagca acccacccaa ccccagaata tgatccagat gcccgtgtgt
     1441 gatgagcgca cgcgttaacc gcttaaagcc cttgccagac tgtaatatct ggcgctgggt
     1501 gccttcgcca caatcgacca aaaaacgata ttcgttatga gagacgatat gagaagaaag
     1561 cccgcggtgt gccgatggcg cagatgcgga tgtaccaaga aatagaattt cgaacaaaat
     1621 tgtgtcctat caaactgcgg aggaaatgta gccccgcgaa tcaatcgctg aaaaatactc
     1681 ttatcgcaca caaccaaact ggcaacaagc cctgcaattt taccttaact caaatcaact
     1741 attgggcaaa aacccattta atgaagaata cgtttgaaat gaaactcaat agaatcagca
     1801 caagaagtta aacagaccat ttgtgctata atgactcaag aacgattttt tatcgtaaat
     1861 gatgaacgaa cctatttcct cttgaaaatc gaatgcctct cctaaaaccc tccgcgccgc
     1921 aatcgaatag taaaaagccc gcaggcaaaa ctcttcccgc aaaaaaaggc ggcgcggatt
     1981 taccttccaa agctccggca aaaggcagag ggcttcctcc tcaagctcca ccatccccac
     2041 ctccatcata tacaccccaa cccctgcgtg aacaaacctg gtgggaaaaa ctttcacccg
     2101 aacgcaagtt agatgttgta ggtgtcatcc tcgcattttt tggtctcgtc attttattag
     2161 ggcttctttc tgccaaccgc tccgctatcg ttggtggcgc gatctttttc cttgcacaac
     2221 tatttggctg gggcgtatac gtcttaccca ttggcttgct cgtctttgga ttgtggctgg
     2281 tcttccgtaa gatcgaacgt atccctccgc tttctacaga acgcgcagtt gggagtgtca
     2341 tccttttctt ctggctgatg accctgttgc actccttcat cgccacagct gaaactgccg
     2401 aagcggttgc actcactggt caaggtggcg ggtatttcgg cggtttggcg caacgcattc
     2461 tttggggagc ctttggcgca ggcggtgcct tcatcgccat gatcgcctgg ctcataattg
     2521 gcgtcgccgt tttgttagat aaacccgtca tcgaactttt cttctggctc aaaccgttga
     2581 tgctaaaact gcgcgacctg attatcaaac ccgcgcctca gactcagccg gtcactgctc
     2641 agccccaatc aagtgatgga ctcacgccga tagattctac tgccatgccc gccatggaca
     2701 tgacgaatgc cgtccccgta cagcccgttc aaactcgcag tggcacaacc atcatcgaat
     2761 ggaaattgcc agacctgaca gatattcttg atgaaggcag tgaagcaacc gtcaacgaag
     2821 atttcattaa gcaacgcgca catctcattg aagagacgct cgcttccttt ggcgcgccag
     2881 cgcaagtagt ggcaatcagc cgtggacctt cgatcacgca atttggggtg gagccgctat
     2941 ttcttgagtc ccgaggcggc gtacggacca gagtccgcgt aagtaagatc gcatccttag
     3001 ctgatgacct cgcccttgca ctagccgcgc cgcgcatccg tattcaagca ccggtaccgg
     3061 ggcatagtta tgttggcatc gaagtgccga atgacgaaat ggcgcgtgta gcgttgcgcg
     3121 atattttaga aggtgaaatt ttcaaagggt ataaaaaacc tttggcattt gcgcttgggc
     3181 gtgacgtggc tgggttgcct gccatctcta gcattgagaa tatgccgcac ttgttaatcg
     3241 cgggtacgac gggttctggt aagtcggtgt gtgtgaactc gattctgact tgtatgcttt
     3301 tatacaacac ccccgatgac ttgcgccttg tgctcgtaga cccgaagcgc gtggagttga
     3361 ccgggtataa cggcgttccg catctgcttg ggcccgtagt cgttgaaatg gatcgtgtga
     3421 tcggtgcttt acaatggatc acacgcgaaa tggacaagcg ctatcacatg ttcgcgcagg
     3481 ttggttcacg taacatcact gattacaacg ccaagatgaa gttgaagggc gaaaagaaat
     3541 taccctatct cgtcatcgtc atcgacgaat tagcagactt gatgatgatc gcaccggatg
     3601 aaaccgaaaa gaccatcacc cgcttggcac agttggcacg tgctaccggc attcatatga
     3661 tcctcgctac acaacgcccc tctgtggatg tggtgacggg tctcatcaaa gccaacttcc
     3721 cggctcgtat cgctttcgct gtggcgtcga acacagacag ccgcgttatt ctcgaccaac
     3781 ccggcgcaga aaaattgctc gggcgcggtg acatgctcta ccaagcacca gatgcccccg
     3841 ctgccgcccg tttgcaaggt gtgttcgtct ctgataatga aattcaccgc ttggtggaat
     3901 attggcaaga acaagccgga acaggcaaac cacaaccctc tgatgcggat gtgccagcca
     3961 ataccattcc tgaaaatgtg ccgctcaaac aagtgccgtt atgggaagag aatcccaaag
     4021 tgagcgggga tccattgttc gatgaagctg tggcgattgt ccgcaaagaa ggcaaaggct
     4081 ccgtgtctat gcttcaacgc cgattgcgca ttggttacac tcgtgcctct cgcatcatgg
     4141 acacgatgga agacaagaag attgtgggtc cgccagaggg tgccacccaa atgcgtcagg
     4201 tgctgaatta tggggatgtg caacc
//
LOCUS       DAOQFT010000171         4212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11393_length_4212_cov_7.008179, whole genome shotgun sequence.
ACCESSION   DAOQFT010000171 DAOQFT010000000
VERSION     DAOQFT010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11393_length_4212_cov_7.008179"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLR93_05615"
     CDS             <1..306
                     /locus_tag="PLR93_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26476.1"
                     /translation="GQTMSLSGAEHLQTITTDGIDGSPLPHGPKPASLGAIIAQFKSR
                     ATKRIWKISEFKETPIWQRNYYEHIIRDETDLQNKTDYIEANPLLWDEDDENPVNVN"
     gene            404..1219
                     /locus_tag="PLR93_05620"
     CDS             404..1219
                     /locus_tag="PLR93_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26477.1"
                     /translation="MKFKRFFPFIVLLLLSCVLLVILPAINFFNSEFIQDPAERLQFS
                     LTQGLKFSALILLFAWIGVGLSSLLTLIKDGRSRKLISWVVYLGFLFFCIITPILGVV
                     FLYGVSYAGSSWQKFPTPPETPKAIAAAGYSTVVIETEEGHYFYCDTSQLELCWQPEN
                     KPSSLIIPVSYGSVEQIEGKPIVPAPGRVVSLVGVDHQDGPNHTEIYYAILKNGSIWY
                     LQLDQSSAGLLAGLSSLIAIPIVFVSSMLFIGMVLAAFLHWLANRIWREKEAA"
     gene            complement(1253..1666)
                     /locus_tag="PLR93_05625"
     CDS             complement(1253..1666)
                     /locus_tag="PLR93_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26478.1"
                     /translation="MHRHKRYRYGPPLFPRPFRGWIWMLLIAFMMFGGRGNWWPGILI
                     LVGVMFLFSSLFRDQRPPEPPPPPMQHPPFDMPPPPPARRADTPPNEPVRRADLLPAT
                     CPNCGGPVRSNDLKWTSSHSVACSYCGSNIETRKE"
     gene            complement(1717..2121)
                     /locus_tag="PLR93_05630"
     CDS             complement(1717..2121)
                     /locus_tag="PLR93_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNH26479.1"
                     /translation="MASKTVYLNWDSESKFVLNDRDNYQLRVNGAGNIGSADLLPMSL
                     IGCSAYDVVEILEKQRQELHQLKVTAEAQQDDTPPWKFRKIHIRYQVIGKGIDLEKAK
                     KAIQLSEEKYCSVYATLRDVIEITHEVEVLEA"
     gene            2175..>2485
                     /locus_tag="PLR93_05635"
     CDS             2175..>2485
                     /locus_tag="PLR93_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNH26480.1"
                     /translation="MIIFLVIAFVFAGLESLALYRNWVKVEWVAKPAVMVCLLIWLFT
                     SVGLEGALLWFGLGLLFSLVGDVLLMISLDTLFLAGLVAFLFAHVFYVIGFNIPLPAI
                     SA"
     assembly_gap    2486..2585
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2721..3692
                     /locus_tag="PLR93_05640"
     CDS             2721..3692
                     /locus_tag="PLR93_05640"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNH26481.1"
                     /translation="MSRPIPADEVRIFSGTSNLPLAKSIAAELDIPLEKTIVKKFSND
                     NLYLQLGASVRYRRVYIVQSLTPPVNDNLMELLMMLDIARDAAASEIHAIIPYYSFGR
                     SDKKDAPRISITARLVADLLRTSGATHVMSMMFHSPQVHGFFRVPTDPLSSRPVFQQY
                     LENRDMSGMIIVSPDMGQAKSAARFAKSLSLPVAAGNKERVSDTEVRISGLVGNQVKG
                     HKRALIYDDEIATGGSIAELTRLLVNEGVEEIMVMCTHGVFTRGGLEKLAAIPQIQEI
                     VTTDTVDITAEKRHPKLTVLSVAKVFADSIRHNFNRQSIGDLFVYGE"
     gene            complement(3743..>4212)
                     /locus_tag="PLR93_05645"
     CDS             complement(3743..>4212)
                     /locus_tag="PLR93_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH26482.1"
                     /translation="AILESAYEREDPLWVASALRAMGRSHDNRWDDSVVSKLLDPDPR
                     VRYAAAEAAGELTIEEAGPIMLKMLEDEEEDDNVTMAAIWSLSQIGGEDARAYILNML
                     DSSDDEDVTEFLEDALENLDFNDELNKFDLLSLDEDDDLSEFDEDDLDDEDDK"
BASE COUNT          951 a          953 c         1056 g         1152 t
ORIGIN      
        1 ggtcagacga tgtccttgtc aggcgcagaa catttgcaaa ctattacaac cgatggcatt
       61 gacgggtcgc ccttacctca tggacccaaa cccgcgtcat tgggcgcaat tattgctcaa
      121 ttcaaatcac gcgctacaaa acgcatttgg aaaatctctg aattcaaaga aacacccatc
      181 tggcagcgta attattacga acacatcatc cgcgatgaga ccgacctgca aaacaaaacg
      241 gactacattg aggccaatcc attgttatgg gatgaggatg atgagaaccc ggtcaatgtg
      301 aactaaaccc taccacgggg gcattgaaga ataggatggg ggtacataaa atcctattcg
      361 tatggcaaac taaaattgtt atttaaaagg ctggcatcaa accgtgaaat tcaaacgctt
      421 ctttcccttt attgttttgc tccttctttc gtgtgtactg cttgttattt tgcctgctat
      481 aaatttcttt aattccgaat ttatccaaga tcctgctgag cggcttcaat tttcgttgac
      541 ccaggggctt aaattttctg cgctcattct tttgtttgca tggatcggcg ttgggcttag
      601 tagtttattg acattgatca aagatgggcg atctcgtaag ctcatttcgt gggtggtgta
      661 tcttggtttt cttttcttct gtatcatcac gccgattctt ggcgttgtat ttttgtacgg
      721 tgtttcatat gctggttcta gttggcaaaa attccccacc ccgcctgaaa caccaaaggc
      781 aatcgctgca gccgggtata gtacggtagt gattgaaaca gaagagggac attatttcta
      841 ttgcgatacg tctcaacttg agttgtgttg gcagccagag aataaaccga gtagcctgat
      901 catccctgtg tcttacggct cagtagaaca aattgaaggc aaaccaattg tccctgcccc
      961 tgggagagtt gtgagtttgg taggcgtaga tcatcaagat ggtccaaacc atactgaaat
     1021 ctattatgcg attctaaaga atggcagtat ttggtatttg caattggatc agagttcagc
     1081 aggtttactg gcaggtcttt cttctcttat tgcaattcct attgtttttg tgagcagcat
     1141 gctctttata gggatggtgc ttgctgcatt cttgcattgg ttggcaaacc gcatttggcg
     1201 tgagaaagaa gccgcataaa aaaagctctg ggtccagaac ccagagcttt ttttattctt
     1261 ttctcgtttc aatgttcgac ccgcaatacg agcaggcgac ggagtgagaa ctggtccact
     1321 tcaggtcgtt tgagcggacg ggaccaccac agttggggca ggtggcgggc aacaggtctg
     1381 cgcggcgcac tggctcattt gggggcgtgt ccgctcgccg ggcgggggga ggcggaggca
     1441 tgtcaaatgg cgggtgttgc atgggcggtg gtggaggctc gggcgggcgt tggtctcgaa
     1501 agagagagct aaagagaaac atgacgccaa ccaagatgag aatgccgggc caccaattgc
     1561 ccctgccgcc gaacatcatg aaagcgatta agagcatcca gatccagccg cgaaagggtc
     1621 tagggaagag gggcggtccg taacggtaac gtttatgtct gtgcatgaaa tatcctttgg
     1681 gtacgcatac tatacgcgcc actctcattg aagttgctat gcttccaaca cttctacctc
     1741 gtgcgtgatc tcgatcacat cgcgcagggt ggcgtacacc gagcaatatt tttcttcgct
     1801 gagttgaatg gctttctttg ccttttcaag gtcgatgcct ttgccaatga cctgataccg
     1861 aatgtggatc tttcgaaact tccaaggtgg tgtgtcatct tgctgcgcct ctgccgtgac
     1921 cttgagctgg tgcagttcct gacgctgttt ctccagaatt tcgaccacgt catatgccga
     1981 acagccaatc agcgacatgg gtaacaaatc ggcggaacca atatttcccg cgccattgac
     2041 gcgtaattga taattgtctc ggtcgttgag tacaaatttg gactctgagt cccagttgag
     2101 atatacagtt ttcgatgcca tatccgcagt atagcatggg atgccttcgt ggtaggatag
     2161 agcaggaggg cactgtgatt atctttttag tgattgcgtt tgtatttgca gggttggaat
     2221 ctctggcatt gtatagaaac tgggtgaaag tggaatgggt tgccaaaccg gcggtaatgg
     2281 tttgcttact catctggtta ttcacctctg ttggattaga aggggcgttg ttatggtttg
     2341 gtttggggtt gctcttttct ttggtgggtg atgtgttatt gatgatctcg ctcgatacgt
     2401 tatttttagc gggactggtg gcattcttat ttgcgcatgt gttttatgtg atcgggttta
     2461 atatcccttt gcctgccatc tcagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnntttaa ttatgggcgt atctataaca tcgccttgta tcatcttggt cagatcatgt
     2641 tgatcgccgg ggtgatcgtg cagtatcacc gctgatttgt gtcataatcg gaatacaatt
     2701 caaggtatat ggagaatcat atgtcgcgtc ccatccccgc tgatgaagtt cgcattttca
     2761 gtggcacgtc taatttgccg cttgccaaaa gcattgccgc agagctggat attccattag
     2821 aaaagaccat cgtgaagaag ttcagtaacg ataatttgta tttgcagctt ggtgctagcg
     2881 tgcgctatcg ccgagtgtac attgtccagt cgctcacgcc gccggtcaac gataatttga
     2941 tggaactgtt gatgatgctc gatattgcgc gcgatgccgc cgcttctgag atccatgcca
     3001 ttattcctta ttattctttt gggcgttccg ataaaaaaga cgcgccgcgc atctccatca
     3061 cagcccgcct cgttgcggac ctgctcagaa cctctggtgc cacgcatgtg atgagcatga
     3121 tgttccactc gccgcaggtg catgggttct tccgtgtgcc tacagacccc ctgagcagtc
     3181 gcccggtctt tcagcaatat ttggaaaacc gcgatatgag cggcatgatc atcgtttcgc
     3241 ccgatatggg tcaagccaag tctgcggcgc gctttgcgaa gagcttaagt ctgcctgtgg
     3301 cggcaggaaa taaagaacgc gtctccgata ccgaagtgcg cattagcgga cttgtcggta
     3361 accaagttaa agggcacaaa cgcgctttga tctatgatga tgagatcgct accggcggct
     3421 ccattgctga gttgacgcgt ttgcttgtga acgaaggcgt ggaagagatc atggtgatgt
     3481 gtacccatgg tgtctttacc cgcggcgggt tggagaaact ggcggcgatt ccgcaaattc
     3541 aagaaattgt aacgaccgat accgtcgata tcactgcaga gaaacgtcac cccaagctga
     3601 ccgttctttc ggtggcaaag gtctttgcag actccattcg ccataacttc aaccgacagt
     3661 cgattggtga cctgttcgtg tacggcgaat aactctcagc gcaaacaaaa agacactccc
     3721 agcaagggag tgtctttttg ttttatttat catcctcatc gtcgaggtcg tcttcgtcga
     3781 attcagaaag gtcgtcatct tcatcgagtg agagcaggtc aaacttgttt agctcatcgt
     3841 tgaagtctaa attttcgagc gcatcttcga ggaattccgt tacatcttca tcatcggagc
     3901 tatcgagcat gttgaggatg taggcacgcg catcttcgcc gccgatctgg ctcagagacc
     3961 aaatggctgc catggtcacg ttatcgtcct cttcttcatc ttcgagcatc ttcagcataa
     4021 taggacccgc ttcttcgatg gtcagttcac cggcggcttc ggcggcagca tagcggacgc
     4081 gtggatcggg gtcaagcagt ttgctgacca cgctatcatc ccagcggtta tcgtgcgaac
     4141 gtcccatggc gcgcagagca gatgccaccc aaagcggatc ttcacgctca tacgccgatt
     4201 caaggatcgc ca
//
LOCUS       DAOQFT010000172         4208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11406_length_4208_cov_6.134842, whole genome shotgun sequence.
ACCESSION   DAOQFT010000172 DAOQFT010000000
VERSION     DAOQFT010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11406_length_4208_cov_6.134842"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLR93_05650"
     CDS             complement(<1..442)
                     /locus_tag="PLR93_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26483.1"
                     /translation="MIKKIIPTLALFAVLLAACGGQPAEPTLSSEQVQGTAISAAFTM
                     VAETQAAIPTNTPVPPTETPSPTPLPTFTPLPLPTQAPIEFPTATTAASSGGGGQCDS
                     VLNEAEGGPKSTVRFQNKTNGVVDLSLYMYSPNKFAQCGSLASNP"
     gene            complement(482..1183)
                     /locus_tag="PLR93_05655"
     CDS             complement(482..1183)
                     /locus_tag="PLR93_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26484.1"
                     /translation="MKRLILVSIVLGLLLSACLPSLSQPQEYPTPVSETDLQATAAIL
                     SQQTLQSIPTNTAIPTETLKIVTPTKTITPTQPTPTETVNPVLLTLTATLLAGTSPAE
                     TQTAVTKTLSTEEITGTPSLTATTVSAVTTSGTPQPAFYGTLPPNLPSGTLGLYNRDH
                     VDVYVSFRCVTKDGYVTILEYPVNKNVNVSAPAGKYTYVAWVGGRQFDGSFTLDNEGF
                     LVISFFKNKVTIKKS"
     gene            complement(1205..1552)
                     /gene="trxA"
                     /locus_tag="PLR93_05660"
     CDS             complement(1205..1552)
                     /gene="trxA"
                     /locus_tag="PLR93_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNH26485.1"
                     /translation="MSGEPIHITDEAFEQVVMQSNLPVIVDFWAPWCNPCKMIAPTLD
                     KLAKELEGKIVIAKVNTDDHAQWMQKFGIQGIPTLLFVSNGKVVHRQVGALPERMLRD
                     VVNQFMEVAGQQA"
     gene            1665..2573
                     /gene="ftsY"
                     /locus_tag="PLR93_05665"
     CDS             1665..2573
                     /gene="ftsY"
                     /locus_tag="PLR93_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNH26486.1"
                     /translation="MSIFSRWREGLSRTSKAAFGQIASILGTSEITDETWDDLEATLI
                     QADLGIETASSVLEDLKRLTRAEGWTRSDELFSALKAELRSRLIQPPAISLNDKPTVI
                     LVVGVNGSGKTTTIAKLSAKFVGEGKKILLGAADTFRAAAVDQLQVWGDRLNVEVIAG
                     APESDPGAVAFSAVQAGVARGVDIVMVDTAGRLHTRFNLMEELKKVNRVVGKALPGAP
                     HQVWLVLDATTGQNALQQARSFKEAVNVSGVILTKLDSSARGGMAFAIQRELGLPILF
                     AGLGEKPEDLTPFDPDAFVDGILVNN"
     gene            2582..3671
                     /gene="prfB"
                     /locus_tag="PLR93_05670"
     CDS             join(2582..2647,2649..3671)
                     /gene="prfB"
                     /locus_tag="PLR93_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114679478.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNH26487.1"
                     /translation="MQDLLQRLNAANETVQQLLERLDLVGKQNQLSQLETEIEHPDFW
                     NDSTAAQKVMKTVSSLRDEVEGWTKLKQQVHDLTDLASLEDETLRAELETEIQKVEKE
                     LEKRSFTMMLSGPFDHDDAILAIHAGAGGTDSQDWAGMLQRMYLRWIESRGFTAEILD
                     MTPGEEAGIKSMTIAVSGQYAFGYMRSEKGVHRLVRLSPFDAAHRRHTSFALVEVLPQ
                     VSMDEADVEINQSDVKMDVFRSSGAGGQNVQKNATAVRLTHIPTGIVVTCQNERSQTQ
                     NREFAMRILRARLLQLKQAEKEEERAVLRGEYTKAEWGSQIRSYVLHPYQMVKDHRTD
                     FQVGNAQSVLDGDLDAFMEDYLKSGDGK"
     gene            complement(3721..>4208)
                     /locus_tag="PLR93_05675"
     CDS             complement(3721..>4208)
                     /locus_tag="PLR93_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNH26488.1"
                     /translation="DMVVKGKVTRLETYGAFVDFGAERPGLIHVSELAHGYVKTAGDV
                     VKAGDEVEAKVLEVDRRKRQIRLSIKALQELAVEEAAEPVEYKGKGKRKGKKQEESYE
                     METEVSNEPQYTAMQIAWQQALDKANGKKNIRAKSIKGTSKEQENLYNSTLEKRLPTG
                     G"
BASE COUNT         1081 a          900 c         1079 g         1148 t
ORIGIN      
        1 aaggattgga tgcaagacta ccgcactgag caaatttatt aggagaatac atgtatagag
       61 acaaatcaac tacaccattg gtcttgtttt gaaaacgtac agtcgatttt ggtccacctt
      121 cagcttcatt caaaacacta tcgcattgtc caccaccgcc agagctggca gcagttgttg
      181 ctgtcggaaa ctcaatagga gcctgagtag gaagaggcaa aggtgtaaag gttggtaagg
      241 gagtagggct tggagtttcc gtaggaggaa caggtgtatt agtcggaatt gctgcttgtg
      301 tctctgctac cattgtgaat gcagctgaaa ttgcagtgcc ttgtacctgt tcagaagaga
      361 gggtgggttc agccggctga ccaccgcatg cagccaaaag cactgcaaac aaagccaggg
      421 ttgggattat ttttttgatc atattgttta ctatctccat ttactttttc gatattgaaa
      481 actagctttt ctttatagtt actttattct tgaaaaatga gatgactaga aagccctcat
      541 tgtccaaggt gaaactacca tcaaattgcc tgccgccaac ccaagcaaca tatgtatatt
      601 tcccagcagg ggcgctgacg ttgacatttt tgtttacagg atattcaaga attgtgacat
      661 agccgtcttt ggtcacacaa cgaaacgaca cataaacatc gacatggtct ctgttataaa
      721 gtcctaaagt acctgatggt agattaggag gaagtgtgcc ataaaaagca ggttgaggtg
      781 ttccgctggt agtaacagcc gatacggttg ttgctgttag gctaggcgtt cctgtgattt
      841 cttcagtcga aagtgttttt gtaacagcgg tttgtgtttc agctggggat gttcctgcaa
      901 gaagggttgc agtgagagtt aatagaacag ggttaaccgt ctcggtagga gttggctgtg
      961 taggcgtgat tgttttagta ggcgtaacga tttttagggt ttcagttggg attgctgtat
     1021 ttgtaggaat ggactgcaaa gtttgttgtg aaagaatagc agccgtagct tgtaggtctg
     1081 tttcagaaac aggggtaggg tattcttgag gctgcgaaag tgatgggaga caggcactta
     1141 ataacaaacc caacacaatg gaaacaagaa ttagcctctt cataccctac tccaacgttt
     1201 taatttaagc ttgctgtccc gcaacttcca tgaattggtt gaccacatcg cgcaacatgc
     1261 gttcgggaag tgcgccaact tggcggtgaa caaccttgcc atttgaaaca aacaacaaag
     1321 ttggaatacc ttgaatgcca aacttctgca tccattgagc atgatcatct gtgttgactt
     1381 ttgcaataac gatcttgcct tctaattctt tggcaagttt atcgagagta ggcgcgatca
     1441 tcttacaagg gttgcaccat ggtgcccaaa aatccacaat aacaggaaga ttggactgca
     1501 taacaacctg ttcaaacgct tcgtcagtga tatgaatggg ttctccggac atttttattc
     1561 ctttttaacc tttatgattc tttcttattg agccatattt agccaaatga gtataacata
     1621 aaaaacatgc catcatatgc ccatagggta taatcgccgc ccatatgagc attttctcac
     1681 gatggcgaga aggactttct cgcacaagca aagctgcatt tggtcagatc gcttccatcc
     1741 ttggtacttc tgaaattaca gatgaaacat gggatgacct tgaagctaca ttgattcaag
     1801 cagacttagg aattgaaaca gcatcatctg tgctagagga cttaaaacgc ctcacgcgcg
     1861 ccgaaggctg gacacgctcc gacgaacttt tttctgcgct gaaagctgaa ctgcgttctc
     1921 gcctaattca accgcctgct atttccttga acgacaaacc aaccgtgatt ttggttgtgg
     1981 gtgttaacgg ttctggcaag acgacaacca ttgccaaact ttctgccaaa ttcgttggcg
     2041 aagggaaaaa gatacttctt ggcgcagcgg acaccttccg ggctgcggcg gtggaccagc
     2101 ttcaggtgtg gggagacagg ctaaacgttg aagtgatcgc tggcgctcca gagagtgacc
     2161 caggtgcggt ggcgtttagc gccgtccagg ctggggtagc tcgcggcgtg gatatcgtca
     2221 tggtggatac tgctggacgt ttacataccc gcttcaacct gatggaagag ttgaagaagg
     2281 tgaaccgtgt agtgggtaaa gcattgcccg gtgcgccgca tcaagtgtgg cttgtgttag
     2341 atgccacaac agggcagaat gctttgcaac aggcacgttc ttttaaggaa gccgtgaatg
     2401 tttctggcgt cattttgacg aaattggact cttctgctcg cggcgggatg gcgtttgcca
     2461 ttcagcgtga attagggttg ccgattttgt ttgcaggctt gggagaaaaa cctgaagacc
     2521 tgaccccgtt tgaccccgat gcctttgtag atggaatttt ggtaaataac taggagtaga
     2581 gatgcaagac ttattacaac gcttgaatgc tgccaacgaa actgtacaac agttattgga
     2641 gcgtctttga cctggtagga aaacaaaatc aactttcgca attagaaact gagatcgaac
     2701 acccagactt ctggaatgac tctactgctg cacagaaggt catgaagacc gtttcaagtt
     2761 tgcgtgatga agtggaaggg tggaccaaac tcaagcagca agttcatgat ttgacagacc
     2821 ttgcctcact ggaggacgag actctccgtg cagagttgga gactgaaatt cagaaggttg
     2881 agaaagaact ggaaaagcgc tctttcacca tgatgctttc gggtccgttt gaccatgacg
     2941 atgcgattct cgcaattcat gctggcgctg gcggaacaga ctcacaagat tgggctggga
     3001 tgcttcaacg tatgtatctg cgttggatcg aaagccgtgg cttcaccgcc gaaattttag
     3061 atatgactcc cggtgaagaa gcaggaatca aaagtatgac catcgctgta agcggacaat
     3121 atgctttcgg gtatatgcgt tctgaaaagg gagttcatcg cctggtgagg ctttctcctt
     3181 ttgacgcagc tcatcgccga catacatcct ttgcgttggt tgaagtgttg ccgcaagtca
     3241 gcatggacga ggcagatgtg gagatcaacc agagcgatgt aaaaatggat gtcttccgtt
     3301 cctctggtgc aggcggacag aatgtgcaga aaaatgccac cgctgtgcgc ttaacacata
     3361 ttccaacagg aattgtagtg acttgtcaga atgaacgttc acagacgcag aaccgcgagt
     3421 ttgccatgcg aattctccgt gcgcgtttgc ttcagttaaa gcaagcagag aaagaagaag
     3481 aacgcgctgt gttgcgcgga gaatacacca aggcagaatg gggcagccaa attcgttcgt
     3541 atgtcttgca tccataccaa atggtgaaag accatcgcac cgattttcag gttggcaatg
     3601 cgcagtctgt gctagatggc gatttggatg cgtttatgga agactattta aagagtgggg
     3661 atgggaagta aattggtaca taaaaagagc ggacatttgt ccgctctttt ttgtttttct
     3721 ttagccgcca gtggggaggc gtttttctaa ggtgctgttg taaagatttt cctgctcttt
     3781 ggaagtaccc ttgatggatt tggcgcggat gttcttcttg ccgtttgcct tgtccaacgc
     3841 ttgctgccaa gcgatttgca tggcagtgta ctgcggttcg ttggaaactt ccgtttccat
     3901 ttcgtaggat tcttcttgtt tcttgccctt gcgcttacct ttgcccttgt attcaacggg
     3961 ttcggcggct tcttcaaccg cgagttcctg aagagccttg atgctcaagc ggatctggcg
     4021 cttgcggcga tctacttcga gaaccttggc ttccacttca tcgccagctt tcacaacgtc
     4081 gccagcagtc ttgacatagc cgtgagccaa ttcgctaacg tgaatcaagc cggggcgttc
     4141 tgcgccgaag tctacaaaag cgccgtaggt ttcgaggcgg gttactttgc ctttcacaac
     4201 catatcgg
//
LOCUS       DAOQFT010000173         4202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11433_length_4202_cov_6.011333, whole genome shotgun sequence.
ACCESSION   DAOQFT010000173 DAOQFT010000000
VERSION     DAOQFT010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11433_length_4202_cov_6.011333"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="menC"
                     /locus_tag="PLR93_05680"
     CDS             <1..866
                     /gene="menC"
                     /locus_tag="PLR93_05680"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNH26489.1"
                     /translation="KDALDFQNHMESIRGHHLAKAGVEMAIWDLLGKRQGKSLKELFG
                     GVRDKVEMGVSVGIQETPEKLVETVTNFVNSGYARVKLKIKPGKDVTYAQAIRKAFPN
                     IRLQVDANSAYSMDDADSLKPLDDLNLLLIEQPLFEDDIWDHHKFQAKFSTPVCLDES
                     IVSPRHARYAIEMKACRIINIKAGRLGGLSQGIQTHDISQQNGMPVWCGGMLETGIGR
                     ASNLALSSMPNFKLPGDVSASDRYYTRDITHERFVLNADSTIDVPNKPGLGVTVDEEA
                     LKSYTLAKVVV"
     gene            970..2637
                     /locus_tag="PLR93_05685"
     CDS             970..2637
                     /locus_tag="PLR93_05685"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNH26490.1"
                     /translation="MKKISLFLFIAILFSACAPATEAPASPAEPTVSAQPTSTQMPVA
                     DALWVSPAMPNVLREAAKSSGVPIVNDAESATQKLDIADSGSLWIYALVAPFPTVTDG
                     ISSADLISAWQNGSSLTGNGQPLLMAESTLRAFTALWGEPASGTVSIVASDQLLTTAW
                     TQPFSWAIIPFEEIQPKWKVLSVDGQSPIHKDFDANLYPLKLSFGLTNSNIELPATNR
                     DASKLATVVLTGVTALVRATAFTMENKGITRPGEFVYNWLHDADVLHISNEVPFDPDC
                     PYPTPGYKNFILCSDPKYMELLTYVGTDIVELTGDHFADRGTQAMLDTLQMYRDNNIP
                     YYGGGANEEEGRKPVLMNVNGNKIAFMGCNGKVSYVFVKATDTKPGAADCDYEFFTQQ
                     VKDVAAQGYMVIFTFQHEECYHHGPCYKHEEGFHAVADAGATIVSGSQAHYPHLMEFR
                     GSTFIHFGLGNLFFDQMTYELPDGSVIDETRREFIDRHVFYDGKYLGTELLTAMLEDF
                     SRPRPMNERERTQFLSEYFAYSGWTELQPTPIPQPTVTLTPIILPTP"
     gene            2767..3309
                     /locus_tag="PLR93_05690"
     CDS             2767..3309
                     /locus_tag="PLR93_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26491.1"
                     /translation="MKNIISRVVLAVLVASLAFAAIPVTSAYAADDTPPAKGELTDEK
                     LEEIWARASQAFGRLGKAFEDTDAHIAKIQSMIDKAAANGKDVSSLQSALDAYKAALV
                     ASKPAYEQLAATMESHAGFDANGKVTDSETAKATLKQVRDEMKALKESMGGTFKALRE
                     AVKAFREANKPAKEPIEKDS"
     gene            3438..3818
                     /locus_tag="PLR93_05695"
     CDS             3438..3818
                     /locus_tag="PLR93_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNH26492.1"
                     /translation="MSKQVIHTEKAPKAIGPYSQAIHIGNLVYTAGQVALDPATMELV
                     AGGVEEQTRQVLANLTNVLEAAGTSMSKVVKTTVFLKTMDDFTKMNAVYAEIFGENPP
                     ARTTVAVSGLPKNALVEIECVALA"
     gene            3819..>4202
                     /locus_tag="PLR93_05700"
     CDS             3819..>4202
                     /locus_tag="PLR93_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26493.1"
                     /translation="MSAELILLVDDEPSITELARMYLERDGYRIHEAADGESALETAE
                     KQKPALIVLDVMLPKVDGFDVCRRLRSAGNDVPIIMLTARDEDIDKILGLELGADDYL
                     TKPFNPRELVARVKAILRRSESKKDS"
BASE COUNT         1122 a         1023 c          998 g         1059 t
ORIGIN      
        1 tcaaagatgc gctcgatttt caaaaccata tggaaagcat ccgcggacat caccttgcca
       61 aagccggtgt agaaatggca atttgggatt tgctcgggaa acgccaaggc aaatcactca
      121 aagagttatt cggcggcgtg cgagataaag tggaaatggg tgtatccgtc ggtattcaag
      181 agacacctga aaaactggtg gaaaccgtca caaactttgt caacagcggg tatgcccgcg
      241 tgaaactcaa aatcaaaccc ggcaaagatg taacctatgc ccaagccatt cgcaaagcat
      301 ttccgaacat ccgcttacaa gtggacgcca actctgctta ttccatggac gatgccgact
      361 cactcaaacc gctcgatgat ttgaacttac tgctcattga acagccgctc tttgaagatg
      421 atatttggga tcatcacaaa ttccaagcca agttcagtac cccagtttgt ctcgatgaaa
      481 gcatcgtctc tccgcgccat gctcgctatg ccatcgaaat gaaagcctgc cgcatcatca
      541 acatcaaagc aggcaggctc ggcggattaa gccaaggcat tcaaacgcac gatatttctc
      601 aacaaaacgg aatgcccgtt tggtgtggcg gcatgttaga gaccggtatt ggtcgcgctt
      661 cgaatcttgc tttatcgtcc atgccgaatt tcaaattgcc cggtgatgtc tccgcgtctg
      721 accgttacta cactcgcgat atcacgcatg agcgtttcgt tctcaatgcc gattcaacta
      781 tcgacgtacc aaacaaaccc ggtttgggcg tcactgtcga tgaagaggcg ctcaagtctt
      841 acacgttggc aaaggtggta gtctaacaac caaactgggt gtaaactaac gctcacgccc
      901 gtttaatcaa ttaagtttac aagtacgagg cgctctgtgc ttcgtacttt tttgcgccat
      961 ggattacaaa tgaaaaaaat atcactattc ctcttcattg caatactatt ctctgcttgt
     1021 gcgcctgcta ctgaagcacc cgcctcgccc gctgaaccaa ctgtctctgc tcaaccaact
     1081 tccacgcaaa tgccggttgc agatgcattg tgggttagcc ctgccatgcc caatgtcttg
     1141 cgagaagctg ccaaaagttc tggtgtgcca atcgtgaatg atgctgaaag cgctactcaa
     1201 aaattagaca ttgccgattc aggctctctt tggatttatg ccctcgttgc gcctttccct
     1261 acagtgacag atggtatctc gtccgcagac ctgatcagcg cctggcagaa cggctcttcc
     1321 ttaaccggga atggtcagcc gttgctcatg gcagaatcca ctttgagagc gttcactgcc
     1381 ctgtggggcg aacccgcttc gggcacagtg agcatcgttg catctgacca gctattaacc
     1441 acagcgtgga cacagccttt tagttgggcg atcatcccct ttgaagaaat ccaaccaaag
     1501 tggaaagtat taagcgtaga cggtcaatcg ccgatccata aagattttga tgcgaacttg
     1561 tatccgttga aactttcgtt cgggctgacg aactcaaaca ttgaattgcc tgctacgaat
     1621 cgtgatgctt caaaattggc aactgtggtg ttgaccggtg tgaccgcact tgtgcgtgct
     1681 acggcattca cgatggaaaa caaagggata actcgtcccg gcgaattcgt ctataactgg
     1741 ttgcatgatg cagatgtgtt gcatatcagc aacgaagtac cttttgatcc cgattgccca
     1801 tacccaaccc cgggctataa aaatttcatt ttgtgcagtg atccgaaata tatggaattg
     1861 cttacctatg taggcaccga cattgtggaa ttaactggcg atcactttgc agatcgcggc
     1921 acgcaagcca tgttggatac cttgcagatg tatcgtgata acaatattcc atattatggc
     1981 ggcggcgcga atgaagagga ggggcgcaaa ccggtcctaa tgaatgtgaa tggaaacaaa
     2041 attgcgttta tgggctgtaa cggaaaagtt tcgtatgtct ttgtgaaagc gaccgatacc
     2101 aaacccggcg ctgcagactg tgattatgaa ttcttcacgc aacaggtcaa agatgtagcg
     2161 gcgcaaggct atatggttat ctttactttc cagcatgaag agtgttatca ccatggacct
     2221 tgttacaagc atgaagaagg ttttcacgcc gtagcggatg ctggcgctac gattgtgagt
     2281 ggcagccagg cgcattaccc gcatttgatg gaattccgtg gcagtacttt tatccatttt
     2341 ggtttgggta atttgttctt tgaccaaatg acgtatgaat tgcccgatgg ttcggtcatt
     2401 gatgaaaccc gccgtgaatt tatcgaccgc catgtgttct acgatggcaa atatctcggc
     2461 actgaattgc tgaccgccat gctcgaagac ttctcccgtc cgcgcccgat gaatgaacgt
     2521 gagcggacac aattcctttc cgaatacttt gcctacagcg gctggacaga attgcagccc
     2581 acgcccattc cacagccaac tgtcacattg acgccgatta ttttgccaac tccataggcg
     2641 aaccaagagc tccgaggttt tcaaaacctc ggagctcttt ataaaattta caaccttttt
     2701 acatccccgc catactcttc taaccgctgg catctagaat ttcatcgtaa cctgaaggag
     2761 gtttcgatga aaaatataat ttcaagagtg gtgttggcag tattggttgc ttcgctggca
     2821 tttgcggcaa tccccgtcac aagcgcgtat gctgcggatg acaccccacc cgcaaagggt
     2881 gagttgaccg atgagaagct ggaagaaatc tgggcgcgcg cttctcaggc ttttgggcgt
     2941 ttggggaaag ccttcgaaga taccgatgcc catatcgcaa aaatccagtc catgatcgat
     3001 aaagcggcag ccaatggtaa agatgtgtct agcttgcaat ctgcccttga tgcttacaaa
     3061 gctgcattgg ttgcgtccaa gcctgcatat gaacaactgg cagccaccat ggaatctcat
     3121 gctggttttg atgccaatgg caaagtgacc gactcagaga cagccaaagc tacacttaag
     3181 caagtgcgcg atgaaatgaa agccctcaaa gagtcgatgg gtggcacgtt caaagccctg
     3241 cgcgaagctg tgaaagcttt tcgcgaagcc aataaacccg ctaaagaacc catagaaaaa
     3301 gattcgtaat ttcaggatat aaaccacaga gggtgccgag aatgttgagc gaaaactcaa
     3361 tgtctccgcg ctctctatgg ctgcctttcc tcgattaaaa taaagaaagc aattaacttc
     3421 agtaaaacga ggaaatcatg agcaaacaag ttattcacac cgaaaaagct cctaaagcga
     3481 ttggacctta ttcgcaagct attcatattg gcaatttggt atacacagcg gggcaggttg
     3541 cgcttgaccc tgctacgatg gaactggtcg ctggtggtgt ggaagaacag actcgccaag
     3601 tgttagccaa cctcaccaat gtattggaag ccgctggtac aagtatgagc aaagtggtaa
     3661 agactacggt ctttcttaag actatggatg attttacaaa aatgaatgct gtctatgcag
     3721 aaatctttgg tgaaaaccca cctgctcgta ctaccgtagc agtttctggt ttaccaaaga
     3781 atgccttggt tgagatcgag tgcgttgccc tagcataaat gtccgctgaa ttaattctgc
     3841 tcgtcgatga cgagccctcc attactgaac ttgcgcgcat gtatcttgag cgtgatggat
     3901 atcgcattca cgaagccgcc gatggcgagt ctgcgttaga gacggcagag aaacaaaagc
     3961 cggctctcat tgtattggat gtgatgctcc ctaaagtaga tggctttgat gtctgtcgtc
     4021 gattacgttc tgcggggaac gatgtgccca tcattatgct cacggctcgt gatgaagata
     4081 tcgacaaaat tcttgggctt gaactcggcg cagatgatta ccttaccaaa ccgttcaacc
     4141 cacgagagtt ggtggcgcgc gtcaaagcca ttttgcgccg tagtgaatcc aaaaaggatt
     4201 cg
//
LOCUS       DAOQFT010000174         4198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11450_length_4198_cov_6.550809, whole genome shotgun sequence.
ACCESSION   DAOQFT010000174 DAOQFT010000000
VERSION     DAOQFT010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11450_length_4198_cov_6.550809"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..579)
                     /locus_tag="PLR93_05705"
     CDS             complement(55..579)
                     /locus_tag="PLR93_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26494.1"
                     /translation="MPLHFTKLANPIPEEVYSAFTKWENDPALVHLTRPNQTQADIDR
                     KSEVDSFELDARISYQMTYLIYLDSQLVGEMSYQVDPQHLYKKETGTAWISITIGEES
                     ARGKGIGVLAMQYLEEEIQKAGYKRIELGVFEFNEPAQKLYQKMGYTEIGRIPNFTFW
                     QNKMWTDIRMEKYI"
     gene            complement(598..885)
                     /locus_tag="PLR93_05710"
     CDS             complement(598..885)
                     /locus_tag="PLR93_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26495.1"
                     /translation="MQITPLHTSIEKLIELKIGVVVMESPNGFPLHETNIYLVAPSGD
                     VVWKAEKPSANVLFTKMKLNEDGSISTFTNSGQFCDINGENGKVISSSNFS"
     gene            1644..1727
                     /locus_tag="PLR93_05715"
     tRNA            1644..1727
                     /locus_tag="PLR93_05715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1678..1680,aa:Leu,seq:cag)
     gene            1998..>4198
                     /gene="gyrA"
                     /locus_tag="PLR93_05720"
     CDS             1998..>4198
                     /gene="gyrA"
                     /locus_tag="PLR93_05720"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNH26496.1"
                     /translation="MAEENMIAGNVESVDIDAQMRSAYLDYAMSVIVARALPDARDGM
                     KPVHRRILYAMHELGLRSNSSYKKSARIVGEVLGKYHPHGDSAVYESMARMAQDFSMR
                     YMLVDGQGNFGSVDGDAPAAMRYTEARLQKMAEEMLQDLEKETVDYGPNFDDSLQEPL
                     VLPARLPNLLLNGASGIAVGMATNIPPQNLRELTSAINYIIDNYDKADEITVDDLMKF
                     VQGPDFPTGGMIVGTEGIVSAYGTGRGRIVMRGIAHIEETKAGRYQINITEIPYQVNK
                     SSLIERIAELVREDKIDQISDLRDESDKRGMSIVIELKRGAQPKKVLNQLYKYTALQS
                     TFGVQMLALVDGEPRTLPLKRLLQIFIEHRQIVITRRSQFDLAKAKARLHILDGYLIA
                     LNNIDAIIKVIREAEDADKAKSALITRFKLSEIQAQAILDMQLRRLAALERWKIEEEH
                     KQVSETIKYLEDLLANPHKILALIKDDMTDVAEKYGDDRRTKISAEAREDIHEEDLVP
                     DESVLISLTERGYIKRVAATAFRQQSRGGRGVKGHTTKDEDEVVMLIPARSLDTMLFF
                     TDKGKVYSEKVYQIPDLDRAQKGIPLVNILALGPNEKVTAAMSVRDFLPTQFCIMMTG
                     RGRIKRVTMEEFASVRPSGLIAMNLDEGDTLGWVRLTSGKDEIIIVTENGQALRFSED
                     KVRAMGRQAAGVQGIRLGKEDAVTSMDVVDKEGALLVVTTNGFGKQTPLKE"
BASE COUNT         1070 a          955 c         1032 g         1141 t
ORIGIN      
        1 ccagagcgag cccagcccaa acaaaccacg gcaggttaag gaaccctttg atcatcagat
       61 gtatttctcc atgcgaatgt ccgtccacat tttgttttgc cagaacgtga agttgggaat
      121 tctgccgatt tcggtatatc ccattttttg atatagcttt tgagcaggtt cattgaattc
      181 aaaaacgccc aattcaatgc gtttgtagcc tgctttttgg atctcttcct ctaaatactg
      241 cattgccaat acgccaattc ccttacctcg agcagattct tcacctatgg tgatgctgat
      301 ccatgcggtg ccggtctctt ttttgtagag atgctgcggg tctacttggt aactcatttc
      361 acctaccagt tgactatcca aataaattag gtaggtcatt tggtaagaaa ttcgtgcgtc
      421 caattcaaac gaatctactt cgctttttct gtcgatatct gcctgagttt ggttgggtcg
      481 agtaaggtgg acaagtgcag gatcattttc ccatttggta aaagcagaat agacctcttc
      541 cgggataggg ttcgctagtt ttgtgaaatg gaggggcatc cgcacttgtt cctgctatta
      601 gctgaaattg gaactactaa ttaccttgcc gttctcgccg ttaatgtcgc aaaactgtcc
      661 actgttcgtg aaagtggaaa tactgccatc ttcatttagc ttcattttgg tgaaaagcac
      721 attggctgaa ggtttttctg ccttccaaac aacgtccccg cttggagcaa ccaaatagat
      781 gttggtctcg tgtaatggga agccatttgg gctttccatc acgacaacac ctattttcaa
      841 ctcaataagc ttttcaatac tggtgtgaag cggagtgatc tgcatgggcg atccttgtgg
      901 aagcgtgatt cgagggcgtt ggatgggtgc tgtttcgaac tacattctct cgtgcgcgta
      961 ttatatctca aactttgttt ccctaccgag cggtagggag tttgaaagtc acaccctttt
     1021 gaaatagcgg taggatccca aatacatatg taacattaat atctcgcctg tcacttattt
     1081 tagacgccta catctattca ataaatctaa ttgtggatta tcttttaatg gaagccagcg
     1141 atgttctcgc tcgcttgaac tatttccccg cttaaaaaag aaaaagccca acgcgttcga
     1201 tcaaatgcgt tgggctaggg gatggggtgg tcatccatca ttgctcttga actcccatgt
     1261 tgctgagttc atcctggctg tattgcgcga ggaacatatc agagcttgcc agggcgagca
     1321 gtagtacaac aaccactgcg gcaaaaacga acattgtgca actcctttct ttttctatat
     1381 caaatataac gattctcttt ttagaaagat actccccttt cagtactgta atggtttgac
     1441 aatacactct tgaaaggtat attctattaa cgcactttta tcataaatag ttccaatact
     1501 acagtggggt tttcactact aaatacaaca tcatcatctt atatttgtgc tgtgatattg
     1561 ttcacataaa gttgaacatc aacaccctct tattgacatt ccacccgttt gggataaaat
     1621 acatcccgcc attcagatga tatgccgaag tggcggaatt ggcagacgcg ctacgttcag
     1681 ggcgtagtga gagcaatctc atgagggttc aagtccctcc ttcggcacac aaagactcgc
     1741 agctgcgggt cttttttgat ggatagaacc cggctcgggc gggggaggag gcggggttta
     1801 tcccattggg cttgccctac gctggatgct taatctgcaa ggcaaaaagc ggataggggt
     1861 ggtataattg cactataaaa ttacgctttc ttcatctaat gtgacacgaa gttcttcccg
     1921 tcttcgtgtc ctttttatgt catttttttg tgaaaaaggc ggaaattcgc tcgtttttgc
     1981 cctcaagagg ataatttatg gctgaagaga atatgatcgc tggaaatgtc gagtctgtag
     2041 atattgatgc ccagatgcgc tcggcatacc tcgattacgc aatgagcgtg atcgtggcgc
     2101 gtgccttgcc cgatgcccgt gacggtatga agccggtgca tcgtcgcatt ttgtatgcga
     2161 tgcatgaatt gggcttgcgc tcgaactcat cttataagaa aagcgcgcgt attgtgggtg
     2221 aagtgctggg taaatatcac ccgcatggtg actcggctgt gtatgagtcg atggcgcgta
     2281 tggcgcaaga tttctcgatg cgttacatgc tggtggatgg tcagggtaac ttcggttcgg
     2341 tggatgggga tgcgccggct gcgatgcgtt ataccgaagc tcgcctgcaa aagatggcag
     2401 aagaaatgtt gcaagacctc gaaaaagaga ccgtggatta tggtcctaac ttcgatgatt
     2461 ctttgcaaga accgcttgtt ctccctgccc gcttaccgaa cttgttgttg aatggcgctt
     2521 caggtattgc tgtgggtatg gcgaccaata ttcctccaca aaacctgcgc gagttgacca
     2581 gtgcgatcaa ttacattatc gacaactacg ataaagccga tgagattacc gtcgacgatt
     2641 tgatgaaatt cgtgcaagga ccagacttcc caaccggcgg catgatcgtg gggacggagg
     2701 ggattgtctc ggcgtatggc accgggcgcg gacgcattgt gatgcgtggt attgcccaca
     2761 ttgaagagac caaagccggg cgttatcaga ttaacatcac cgaaattccg tatcaggtca
     2821 ataaatccag cctgatcgaa cgcattgctg aacttgtccg tgaagataag attgaccaaa
     2881 tttcagacct gcgcgacgaa tcagacaagc gtggtatgag cattgtgatc gagcttaagc
     2941 gtggtgctca gcctaagaag gttttgaacc agctttataa atacacagct ttgcagtcca
     3001 cgtttggggt gcaaatgctg gcattggtcg atggggagcc gcgtactttg ccgctcaagc
     3061 gtctgttgca gatcttcatt gaacatcgcc agatcgtcat cacccgccgc tcacagtttg
     3121 acctcgccaa agccaaagcc cgcttgcata ttttggatgg ctatcttatt gcgctcaata
     3181 acattgacgc cattattaag gtcattcgtg aagcagaaga tgcagacaaa gccaagtctg
     3241 cgttgataac ccgctttaag ttgagcgaaa ttcaagcaca ggcaattctc gacatgcagt
     3301 tacgtcgcct tgcagctctc gaacgctgga agatcgaaga agaacacaag caggtttctg
     3361 aaaccatcaa atatttggaa gacttgcttg ccaacccgca taagatcctc gctcttatca
     3421 aagacgatat gaccgatgtg gcagaaaaat atggcgatga ccgccgtacc aaaatttctg
     3481 ccgaagctcg cgaagatatt cacgaagaag accttgtgcc cgatgaatcg gtgctcatca
     3541 gtcttaccga gcgtggatac atcaaacgtg tggcggcaac tgccttccgc cagcaaagcc
     3601 gtggtggacg tggtgtaaag gggcacacaa ccaaagacga agacgaagtt gtaatgctta
     3661 tccctgctcg cagcctcgac acgatgctgt tctttaccga taagggtaag gtgtactccg
     3721 aaaaggtgta tcaaatccca gacctcgatc gtgctcagaa gggtattccg ttggttaaca
     3781 tccttgcgct gggaccgaac gaaaaggtga cagcggcaat gtcggtccgc gatttcttgc
     3841 cgacccagtt ctgcatcatg atgaccggac gtgggcgtat caagcgtgta acaatggaag
     3901 aattcgcctc agttcgcccc tctggcttga ttgccatgaa cttagatgaa ggggacactc
     3961 tgggctgggt tcgcctcact tctggcaaag atgagatcat catcgtcacc gaaaatggac
     4021 aagccttgag attcagtgaa gataaagtcc gtgcgatggg gcgtcaagcc gcgggtgttc
     4081 aaggcattcg ccttggcaag gaagatgccg tcactagcat ggatgtggtg gataaagaag
     4141 gcgcattgct ggttgtgacc acaaatggat ttgggaagca gacacctttg aaagaata
//
LOCUS       DAOQFT010000175         4191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11480_length_4191_cov_5.692698, whole genome shotgun sequence.
ACCESSION   DAOQFT010000175 DAOQFT010000000
VERSION     DAOQFT010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11480_length_4191_cov_5.692698"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLR93_05725"
     CDS             complement(<1..292)
                     /locus_tag="PLR93_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26497.1"
                     /translation="MNNKVPSFGQLYSTDYNIKSSVQMIFGSLLFQVIAYYGFEQQIV
                     PPLVAILAALTVIGIIWLVTRYNLITSTFRDGITVTGKILDLETITSHSGKNN"
     gene            complement(304..1515)
                     /locus_tag="PLR93_05730"
     CDS             complement(304..1515)
                     /locus_tag="PLR93_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNH26498.1"
                     /translation="MKYTSLSIQTQREFPNNARTQGFGWLVRAGYMTRENQLLPLGEK
                     AIEKLKTQSTEVTFLSSLELPLISNADETFFESAIGSQEIIYCQSCGYIARRELAQTR
                     KTPFAQEEPLPIEKIATPDTNTIESLANLLNIPKEKTAKALMYTRISDGRFVFIVVRG
                     DMQLSEPKLKALVGDVRLATAEEIIGVGAVAGYASPVGLKKEALIVVDDLIQQSSNLV
                     AGANEAGFHLLNTNSGRDYTPEIVADVTLAREGGACAQCGEALSVRRADILASRGESF
                     FDEILLALAETYHDDKGLTLPTFASTFDVYLMHVPGKTMDTKAKAEEIYNALQNAGIT
                     VLFDDRDERAGVKFNDADLIGCPVRLTVGEKGLQNGMVELKRRASSENGTVSVDSLLN
                     SKDINLLISKN"
     gene            complement(1558..2094)
                     /locus_tag="PLR93_05735"
     CDS             complement(1558..2094)
                     /locus_tag="PLR93_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNH26499.1"
                     /translation="MFKRNNTPAEPVQPVQAVERITSVLGSGVIWHGSINGSGGVRIE
                     GAFEGEIALRGMLVVGETGRVTCQNVRANTVIVAGAVRGNITTQKLEIRATGRVWGDV
                     VTTAFVTEEGAFLRGQIRMEESVDLNLEPAPEATPSETAQAEVIAQTPIQIPEPAPVP
                     ASTPVSTQRLPRKKKTEE"
     gene            complement(2159..2953)
                     /locus_tag="PLR93_05740"
     CDS             complement(2159..2953)
                     /locus_tag="PLR93_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNH26500.1"
                     /translation="MGLLEGKNALIFGLANDKSIAWGIIQAFLREGANIGISYAGEIM
                     EKRVKPLAEKINCQWVEECDVTKDDQIEAVVKKAEKHFGKIDILVHSIAYAGRDELSK
                     PYHQTSREGFRVAMDISVFSFVALTNAFLPILNPGASVLCLTYGSGAVKVAPHYNVMG
                     VAKAALESSTRYLAYDLGAQKIRVNAISAGAIRTLAAAGVGGFRDMYKHFADKSPMRE
                     NVTIEDVGDAAVFLASDLSKRITGEVLYVDSGFNIMGVQMVEEKGE"
     gene            complement(2966..3538)
                     /locus_tag="PLR93_05745"
     CDS             complement(2966..3538)
                     /locus_tag="PLR93_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNH26501.1"
                     /translation="MTLPRKFYNRPTLTVARELLGARLVRILDGVKLVGLIAETEAYF
                     GFDDLASHAKAGRTIRTDPMFGPPGHAYVYFTYGNHWMLNAVTEKEGFPAAVLIRAIQ
                     PIEGMDVMMKRRQGRDTFGPGKLTQALGITKSENYADLTESGSSLWIEAGVKVPEKSV
                     TIGARVGLNTTPEPWFSKPWRFLVKNWKVV"
     gene            complement(3548..>4191)
                     /locus_tag="PLR93_05750"
     CDS             complement(3548..>4191)
                     /locus_tag="PLR93_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH26502.1"
                     /translation="TVALMEAMRDVGVKRVVLASSGAVYGDMNDGSLKETDIPNPRSP
                     YAVSKISAEHYVHTIGNLWNIETVSLRIFNAYGPGQHLPPSHPPVVPHYLRQALRGGT
                     LVAHGDGSQTRDYIYVDDVISAMVAASTAPNLNGSVINVGSGMETSIKELINVVLSVT
                     NSKANVVYNMQTSGGVSRMKADLTLAKEKLRFAPSISLVDGLKLTLQRDTRFK"
BASE COUNT          995 a         1084 c         1048 g         1064 t
ORIGIN      
        1 gattgttttt gccgctatgt gaggtgattg tttcaaggtc gaggattttt ccagtgacag
       61 ttattccatc gcgaaaagtg gatgtgatga ggttgtagcg tgtcacaagc cagataatgc
      121 caattacagt taaagcagca aggatggcaa ccaaaggcgg aactatctgc tgttcaaaac
      181 catagtaagc gatcacttga aaaagcaaag agccaaaaat catttggaca gacgacttga
      241 tattgtagtc agtggaatag agctgcccaa aagatggaac tttattgttc atattgtcac
      301 ctcttaattt ttgcttatga gtaaattaat atcctttgaa ttcaagagac tatcaactga
      361 taccgtgccg ttttcactgg atgcgcgtcg tttcaattct accattccgt tttgaagccc
      421 tttttcaccg acggtcagac gcacaggaca gccaatcagg tcggcatcat tgaatttgac
      481 tccggcacgc tcgtcgcggt catcgaacaa gactgtgatg ccagcatttt gtagggcgtt
      541 atagatctct tcggctttgg ctttcgtgtc catcgtcttg ccggggacgt gcattagata
      601 cacgtcgaat gtagaagcaa atgtaggcag tgtcagtcct ttgtcatcgt gataggtctc
      661 agcaagggca agcaaaatct catcaaagaa agactctcca cggctggcaa ggatatcagc
      721 ccgacgaact gacaacgcct cgccgcactg cgcacaggca ccgccttcgc gtgcgagcgt
      781 cacatccgcc acaatttcag gcgtgtagtc acgtccgcta ttggtgttga gcaaatggaa
      841 gccagcctca ttggcacctg ccacgagatt cgacgattgc tgaatgaggt catcgacgac
      901 aatcaacgcc tctttcttta acccaactgg ggatgcatac cccgcaacgg ctcccacgcc
      961 gataatttct tctgctgtcg ccaagcggac gtctccaacc aaagccttca acttcggttc
     1021 gctcaattgc atgtcaccgc gcacgacgat gaaaacaaat ctgccatcgg atatgcgggt
     1081 gtacatcaac gccttggctg ttttttcttt gggaatgttc agcagatttg caagcgactc
     1141 gatggtattc gtatcgggtg tggcgatctt ttcaataggc agaggttctt cttgcgcaaa
     1201 tggagttttg cgagtttggg caagttctct acgggcgata tatccgcatg attggcaata
     1261 gatgatctct tgtgagccaa tcgctgactc aaaaaaagtt tcgtctgcat tggagatcaa
     1321 gggtaattcg agcgaactca agaacgttac ttctgttgac tgtgttttca gtttttcaat
     1381 ggctttttca cccaagggca aaagctgatt ttcacgggtc atatacccgg cgcggacgag
     1441 ccagccaaat ccctgtgtgc gggcgttgtt gggaaattca cgttgagttt gaatggaaag
     1501 ggaggtgtat ttcatataca aattcccctc tctttttagg agagggggag agtgagatta
     1561 ttcttctgtt ttcttcttgc ggggcaatcg ttgtgtgcta acaggagtgg atgccgggac
     1621 aggtgccggc tcagggattt ggattggtgt ctgcgcgatg acttcggctt gagcggtttc
     1681 cgaaggcgtg gcttcaggag caggctcgag attaaggtcg accgattctt ccatccgaat
     1741 ttgaccgcgc aggaacgcgc cctcttccgt cacaaaggcg gtggtgacca cgtcacccca
     1801 cacacgcccg gttgcacgga tttcaagctt ttgggtggtg atgtttccac gtaccgcacc
     1861 tgccacaatg accgtgttgg cgcgcacgtt ctggcaggtc acacgcccgg tctcgccgac
     1921 cacgagcata ccgcgtaggg cgatttcgcc ttcgaatgca ccttcaatgc gtacaccgcc
     1981 ggacccattg atggacccat gccagattac gccagagccc agcaccgaag tgatgcgctc
     2041 tacggcttgg acgggctgta caggttctgc gggagtattg tttctcttaa acatggaggc
     2101 aattttaccg ttaaaacaaa aagggcgagc atcatgctcg cccttgaaat tagctttctt
     2161 attcgccttt ttcttccacc atttgcacgc ccatgatgtt gaagcccgag tctacgtata
     2221 gaacttcgcc tgtgatgcgc ttcgacagat ctgaagccag aaagaccgcg gcatcgccaa
     2281 catcttcaat ggtgacattc tcgcgcatgg gagatttatc tgcaaagtgt ttgtacatat
     2341 cacggaagcc acctacacct gccgcagcca gggtgcggat ggcgcctgct gaaatggcat
     2401 tgacgcggat cttctgtgca ccgaggtcat atgctaaata gcgcgtagag gattccaagg
     2461 ctgctttggc gacgcccatc acattgtagt gaggggctac cttcaccgca cccgagccat
     2521 aggtcaggca taagaccgaa gcaccggggt tcaaaatagg caggaaggca ttggtcaacg
     2581 ccacgaaaga gaagaccgaa atatccatcg ccacacgaaa gccttcgcgg ctggtctggt
     2641 gatagggttt gctcaattca tcgcgcccgg catacgcaat cgaatggacg agaatatcga
     2701 tcttgccaaa atgcttttcg gctttcttca ccacggcttc gatctggtcg tccttggtca
     2761 cgtcacattc ttcgacccac tgacaattga ttttttcagc cagcggcttc acgcgttttt
     2821 ccatgatttc accagcatag ctgatgccga tgttggcacc ctcgcgcaaa aaggcttgaa
     2881 tgatacccca agcaatggat ttatcgtttg ccaacccgaa gatcagcgca ttttttcctt
     2941 caagtaatcc catagtctct cctcgttaaa caaccttcca attttttacc agaaaccgcc
     3001 aaggcttact gaaccaaggt tccggcgtag tatttaaccc cacacgtgcg ccaatcgtca
     3061 cgcttttttc ggggacttta acccccgcct cgatccatag acttgagcct gattccgtta
     3121 aatcggcata attctcgctc ttggtgattc ccagcgcctg agtcagtttc ccgggtccga
     3181 acgtatcccg cccctgacgg cgcttcatca tgacatccat gccttcaatg ggctgaatag
     3241 cgcggatcag cactgccgct ggaaagcctt ccttctccgt aacggcattc aacatccaat
     3301 gattgccgta ggtgaagtac acataggcat gccccggcgg accaaacatc gggtccgtgc
     3361 gaattgtccg cccggctttg gcgtggctgg caaggtcatc aaacccaaaa taggcttcgg
     3421 tctcagcaat caagccgact aatttgaccc catccagaat ccgaaccaag cgtgccccaa
     3481 gtaattcgcg ggcaacggtt aaggtagggc ggttataaaa tttgcgaggg agggtcattt
     3541 aagcggatta cttaaaccgt gtatcgcgtt gaagtgttaa cttcaaccca tctaccaaac
     3601 taatagacgg agcaaagcgc agtttttctt ttgcgagggt caaatctgct ttcatgcgcg
     3661 agacgccgcc agaagtttgc atgttgtaaa ccacattggc tttgctgttc gtcacactca
     3721 agaccacatt gatgagttcc ttgatggacg tctccatgcc cgagccgacg ttgatgaccg
     3781 atccgttcaa gtttggtgct gtagatgctg ccaccatggc gctgatcacg tcatcaacat
     3841 aaatataatc gcgagtctgg ctgccatcgc cgtgcgctac caacgtcccg ccgcgaagcg
     3901 cttgccgcag gtagtggggg acaacgggag gatgtgaagg cggcaggtgt tgccctggtc
     3961 cgtaggcatt gaagatccgc aagctgacgg tttcaatgtt ccacaagttg ccgattgtgt
     4021 gaacgtaatg ttcagccgag atttttgaaa ctgcataggg tgagcgcgga ttggggatat
     4081 ctgtttcttt caaactacca tcgttcatgt cgccatacac tgcgccggaa gaagcgagca
     4141 caacgcgttt aacgccaaca tctcgcatgg cttccatcaa ggctaccgtc c
//
LOCUS       DAOQFT010000176         4178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11538_length_4178_cov_6.426631, whole genome shotgun sequence.
ACCESSION   DAOQFT010000176 DAOQFT010000000
VERSION     DAOQFT010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11538_length_4178_cov_6.426631"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2882
                     /locus_tag="PLR93_05755"
     CDS             <1..2882
                     /locus_tag="PLR93_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26503.1"
                     /translation="LSWLGIPLIYKNELMGVLAVEKWQANFYTREQMQAGLTFASQAA
                     VSLDNSRLFEDSVRRATELDQRSQRLTALNRFTSTLTRSLDTDQILTATAEELFQGLG
                     ASRVSIVTFERGQNLWKYTLPRIRAKLPRMLPDAPIFSRLRESQGIFNTDDVRSEPDL
                     VSILEMLGDQARALLILPLVSGGSLNALVFVQLMGETRFGINELEVARTITNQASIAL
                     ENARLYQSSVRMADRFAVLNETSSLVSASLNPEEVYVSVHHAAERLMPLDSFVITLFD
                     ETANEVDAVYLYDRGKRLPGEKVPFGKGMSSKVISSGKPFLIVDATKDTATDYVQAGD
                     EADTTKSILAVPMILGGKALGMLSAQSYQTGVYSEEDVQLLGTLANQAIVAIQNGRLF
                     NETQKLAAELEMRVLDRTAQLQREKQNTDTLLRILTEVSSSLDLDRALNRTLSLLNDA
                     SGAEQGTILLVHAEDNLLHYRAGYGYLSDRTSSSNKGFTLKIGEGLAGWVVKHRQSVL
                     INDLFEDPRWVRSTATGQDHRSAIAVPMSVGEDAIGVLMVFHRAKDFFSPELLNLVTA
                     IAGQVAVAINNARLYELIRDQAERLGLMLRKEQVEASRSQAILEAVADGVLVTGADNR
                     VSFVNSSITRVLGVDDTQLLGKSLDGFTGLFGSSSTEWIKTIRRWSEDPSTYKVGEMY
                     AEQLELENGRIVLIHLAPVILIHDFLGTVSIFRDITREVEVDRLKSEFVATVSHELRT
                     PMTAIKGYVDILTMGAAGALNENQMHFLEVVRNNIERLNILVSDLLDISRIESGRVKL
                     EQGKVNLYDIAEEVVADVLRRSQNESKPIALSLDAPKNLPPIQGDGARVRQIISNLVN
                     NAFNYTPENGAILVNIHDADGEVQVDVQDNGIGIKPEDQDRLFERFYRGEHPLVLATP
                     GTGLGLPIVRQLVEMHNGRIWMKSTGVPGEGSIFSFTLPVYKNGA"
     gene            2889..3263
                     /locus_tag="PLR93_05760"
     CDS             2889..3263
                     /locus_tag="PLR93_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26504.1"
                     /translation="MARILIAEDEPDIRELVAFTLRFAGHEVTTTANGEEAYHKAQEM
                     IPDLIMMDVRMPKMTGYDACRAMKQIPSLKDIPVVFLSAKGQDSEIQTGLDAGAEEYL
                     LKPFAPDQLVERVKAILAKFGK"
     gene            3264..4052
                     /locus_tag="PLR93_05765"
     CDS             3264..4052
                     /locus_tag="PLR93_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH26505.1"
                     /translation="MSAIILDGSMVHYEALGRGRPIIFLHGWVGSWRYWINAMQAAST
                     SFRAYAIDLFGFGDTVRSENNYTIEKQALLLERFLNEMGIGKVALVGHDLGALVAFEY
                     LKKHYESVDRMMTIGVPLQYETLATRMRTAPTTELAEWLSARQPDALTALADASKADP
                     VAVTTSMSSLQVDGIFPSVRNFGVPCLFVYGANDPAFTTVPSQETSNLPIHMHQINLE
                     GSGHYPMLDNVSQFNRLLIDFLALDSGASPSELQMKEEWKRRVR"
BASE COUNT         1029 a          989 c         1066 g         1094 t
ORIGIN      
        1 gcctttcctg gttgggcatt ccgcttattt ataaaaatga attgatgggt gtgctggctg
       61 ttgaaaagtg gcaggcgaat ttctataccc gcgagcagat gcaagccggg ttgacctttg
      121 cttcacaggc agcagtttca cttgataact cgcgcctctt tgaagacagc gtgagacgtg
      181 ctactgaatt ggatcaacgt tcacaacgtc tcacagcgtt gaatcgcttt acatccacac
      241 tgacacgctc gcttgatacg gatcaaattc ttactgcaac tgcggaagag ttgttccaag
      301 gcttgggtgc cagccgcgtc tctattgtga cctttgaacg cggacaaaac ttgtggaaat
      361 acaccttgcc gcgaattcgt gccaaacttc cgcgtatgct gccagatgct cctatcttta
      421 gtcgcttgcg cgaatcacaa ggtatcttca atactgatga tgtccgcagt gagccggatc
      481 ttgtatccat tcttgaaatg ttgggagacc aggctcgggc attgctgatt ttgcctttgg
      541 taagtggtgg ttcgctaaat gctttggtct ttgtgcagct tatgggcgag acccgttttg
      601 gtatcaatga gttggaagtt gcacgtacga tcaccaatca ggcaagtatt gcgttggaaa
      661 atgctcgctt gtatcaatcg tctgttcgta tggcagaccg gtttgcggtg ctcaatgaaa
      721 ccagttcctt agtgagcgcc agcctcaacc ctgaagaagt atatgtttcc gtccatcatg
      781 cagcggaacg tctcatgccg cttgactcgt ttgtgatcac cctgtttgat gaaactgcaa
      841 acgaggtaga tgctgtttat ctttatgatc gcggcaagcg tttgcctgga gaaaaggttc
      901 cgttcggtaa agggatgagc agtaaagtca tcagctctgg caagcccttc ttgatcgtag
      961 atgctacgaa agatactgcc actgattacg tccaagctgg ggatgaggcg gatactacca
     1021 agtctattct tgctgtgcca atgatcttgg gtggtaaagc cttgggtatg ctttctgctc
     1081 aaagctacca gacgggcgtg tactcagaag aagatgtgca attgcttgga accttggcga
     1141 accaagctat tgttgctatc cagaacggac gtctcttcaa cgagacgcag aaattggcgg
     1201 ctgaacttga aatgcgcgtg ttagaccgca cagctcaatt gcagcgtgaa aaacaaaaca
     1261 cggatacgct cttacgcatt ttgactgagg tatcttccag cctcgatctt gaccgtgctt
     1321 tgaatcgcac gctctcgttg ttaaatgacg cttctggcgc agaacaaggc acgattttgc
     1381 tcgtccatgc agaagataat ttgctgcatt atcgcgcagg gtatggatat ttgtctgacc
     1441 gtacttcttc gtcgaataag ggctttacct taaagatcgg tgaaggtttg gcgggttggg
     1501 tcgtcaaaca tcgtcagtcg gtgcttatca atgatttgtt cgaagacccg cgttgggtac
     1561 gcagcacagc gacaggtcaa gatcaccgaa gcgcaattgc tgtacccatg tcggtgggtg
     1621 aggatgccat cggtgttttg atggtcttcc atcgcgccaa agatttcttc agccctgaat
     1681 tgctcaacct ggtgactgcg attgcaggtc aggttgcggt ggcgatcaac aatgcccgct
     1741 tatatgagtt gatccgcgat caggcagagc gcctcgggtt gatgttgcgc aaggaacaag
     1801 tggaagcgag ccgctcgcaa gccatccttg aagcggtggc agatggcgtg ttggtaacgg
     1861 gcgcagataa tcgcgtctca ttcgtgaact catccattac tcgcgtgctt ggcgtggacg
     1921 atacccaatt gcttggtaaa tcgcttgatg ggtttactgg tttgttcggt agctcttcca
     1981 ccgaatggat taagaccatt cgccgttggt ctgaagaccc atccacatac aaagtgggtg
     2041 agatgtatgc agaacaattg gaattggaaa atggacgcat cgttcttatc cacttggcac
     2101 cggtgatcct catccacgac ttcctcggca ccgtatccat cttccgtgat attacccgtg
     2161 aagtggaagt ggatcgcctc aaatctgaat ttgtggcgac ggtcagccat gagttgcgca
     2221 cacccatgac cgctatcaaa ggttatgtag atattctcac catgggcgcg gcgggtgctt
     2281 tgaatgaaaa ccagatgcac ttcctcgaag tggtgagaaa taacattgaa agacttaaca
     2341 ttctcgtgag cgatttgctt gatatttcac gcattgaatc tggacgcgtg aaacttgagc
     2401 aaggcaaggt caatctgtat gatattgctg aagaagtggt ggcagatgtt ttacgccgct
     2461 cacaaaatga aagcaagccg attgcacttt cactagatgc gccgaagaac cttccaccga
     2521 ttcaaggcga tggcgcaaga gtccgccaga tcattagcaa ccttgtaaac aatgcattta
     2581 attacacccc cgaaaatggc gccattctgg tgaatatcca tgatgctgat ggcgaagtgc
     2641 aagtagacgt gcaggataac ggcattggca taaagcctga agaccaagac cgcctctttg
     2701 aacgttttta ccgtggcgag catccacttg tgttggcaac gccgggcact ggtttaggtt
     2761 tgcccatcgt tcgtcagttg gtcgaaatgc acaatggacg tatttggatg aaaagcacag
     2821 gtgtccctgg tgaaggaagt attttctctt tcaccttgcc tgtctataaa aacggagcat
     2881 aaatttcaat ggcaagaatt ttgatcgcag aagatgaacc ggatattcgt gagttggtgg
     2941 cgtttacgtt acgctttgcg ggtcatgaag tgaccaccac tgccaacggc gaagaagcct
     3001 atcacaaagc acaggagatg attccagacc ttatcatgat ggatgttcgt atgccgaaga
     3061 tgacgggcta tgatgcctgc cgcgccatga agcagattcc atctttgaaa gatattcctg
     3121 tggtgttcct ctctgcaaaa ggacaggact ctgaaattca aaccgggctt gatgcaggag
     3181 ccgaagaata cctgcttaaa ccttttgccc cagaccagct tgtggaacgg gtgaaagcga
     3241 tccttgcgaa atttggaaaa taaatgagcg caattattct tgatggttcg atggtgcatt
     3301 acgaggcgtt gggacgtggt cgtcccatca tcttcctgca cggctgggtt ggctcatggc
     3361 gatattggat caatgccatg caagccgcat ccacatcgtt ccgcgcctat gccatcgatc
     3421 tctttggttt tggcgatacc gttcgcagtg agaataatta caccattgaa aaacaagctc
     3481 tgttgcttga acgcttcttg aatgaaatgg gtattgggaa ggtcgctttg gtagggcatg
     3541 accttggcgc gctcgtcgct tttgaatacc ttaaaaagca ctacgagagt gtagaccgta
     3601 tgatgacaat cggcgtaccg ttgcaatacg aaacacttgc cacacgcatg cggactgccc
     3661 ccacaaccga acttgcggaa tggctgtctg cgcgccagcc tgatgccttg accgctctcg
     3721 cagacgcctc caaagcggac ccggtggctg ttaccacctc catgagcagt ttacaagttg
     3781 acggtatttt cccgagcgtt cgtaattttg gtgtgccatg tctttttgta tatggtgcca
     3841 atgacccagc cttcacgacc gtcccttcac aagagacaag taatctaccc attcatatgc
     3901 accagattaa cttggaagga tctgggcatt accctatgct tgataacgtc tcgcaattca
     3961 accgcctcct gattgatttc ctcgcactcg actcaggcgc cagcccaagt gaactgcaaa
     4021 tgaaagagga atggaaacgc cgcgttcgct aaaaataccg tgagaaaaaa tctcgcggta
     4081 tttttttact tgacattccc acgtcacagt cctatactcc aaacacaacc gaacggagag
     4141 cctttacgaa aggctgagag gagcgtgaac gcgctcga
//
LOCUS       DAOQFT010000177         4154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11634_length_4154_cov_7.909734, whole genome shotgun sequence.
ACCESSION   DAOQFT010000177 DAOQFT010000000
VERSION     DAOQFT010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11634_length_4154_cov_7.909734"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLR93_05770"
     CDS             complement(<1..215)
                     /locus_tag="PLR93_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH26506.1"
                     /translation="MIEVKKLVKRFGLKTILKGLDFSVQPGEFVALLGPNGAGKTTFL
                     RILASLSRPSLGNVKVAGYSLPNESAKV"
     gene            complement(278..2173)
                     /locus_tag="PLR93_05775"
     CDS             complement(278..2173)
                     /locus_tag="PLR93_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNH26507.1"
                     /translation="MKLRHLFLLAIGTLLLSACNMTLAADVTPPPGYVSPTPPPTLGP
                     LYPDKAPDVENGKAIFTEKCAACHGEKGLGDGEQSKDLPVSVIPIGLPEFANEATLSS
                     WYAVVTQGRIERFMPPFNSLTDQERWDVVAYALTLHTTNEQLELGKKLFEENCADCGD
                     AFTNLQMMSALSEDDLVKFVREGNGNFPAFGSNFSDEEAYAVAAYIHTLTFAVPSAPV
                     AAAATETPAAAESTPSTDSTPADGTPQAEGTPAATEAAVASMVKGFIINNSSADLSAD
                     QQITLRGFDHGSDPSAGPTEVLNVQGTVNPDGSYSFDVELLEGQIYLAELDVDGITYQ
                     SEFAVVPAGATELALPDITVYDTTTELTDLVIDSIAVHFDYTDPESISAFVVYLLRNT
                     GDKNIKVQLENNNQDIPFIKAPANAKLLGFQQTQDGATLLATKEQDGFYIQPSDQTYG
                     LIAFSSLPRTNKVDFSQEFLLPVGSGKVFLPNGVTAQGDQLTDQGMVPLQDSSGKNYE
                     YQTYSFTNVSANSTLDFTLKGDPSETSATADLTQNQYVLIGVGAFGITLILAGVFMYL
                     RDRKKAAEESEEAEDDEEDDDQFDDTESIMDAIIVLDDLHRAGKISDEAYKKRRAELT
                     SSLKKKG"
     gene            complement(2193..2702)
                     /locus_tag="PLR93_05780"
     CDS             complement(2193..2702)
                     /locus_tag="PLR93_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH26508.1"
                     /translation="MELGSIFLILAVLVAVGMYLYAPFMYRSRKLSTDEMHEISALKA
                     ERDRVINSLQELDFDFKLGKIPAEDYPEQRAELLKKGSDILRKLDELEPILSSARDAA
                     SRIEKAAAAKRADSSSTASDMSDEDIEAMISARRKQHKSKSAGFCPSCGKPVLTADNF
                     CPSCGKSLK"
     gene            complement(2712..3281)
                     /locus_tag="PLR93_05785"
     CDS             complement(2712..3281)
                     /locus_tag="PLR93_05785"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNH26509.1"
                     /translation="MTETTAPARKSVPLWVQVTIWVFLAGLLALVGFGLNRASHPMAE
                     VGDTVPDFKLVYYEGYEHNNTPEMKLSDLRGKVVLINIWASWCKPCEQEAPELEQAWQ
                     LYKDQGDVVFIGVDYVDTPEGAFGYLKKFGITFPNAPDLQSNISSILNRQMGVPETYL
                     IDRDGVLRQIKIGPFASVSEIQAFIASAE"
     gene            complement(3314..3805)
                     /locus_tag="PLR93_05790"
     CDS             complement(3314..3805)
                     /locus_tag="PLR93_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNH26510.1"
                     /translation="MKKFLFILTLMLVASGLFTAVAFAQDKTPTDDEVNAIAKQLYCP
                     VCENTPLDVCPTEACHQWRELIRQMLAEGKSEQQIKDYFVANYGARVLSEPPRAGFNW
                     LVYIIPPVLILVGAVFLFNAFRAWTKPKSAEAGSGQAEEAGTSSSDDEYIKRLEEELK
                     NRK"
     gene            complement(3859..>4154)
                     /locus_tag="PLR93_05795"
     CDS             complement(3859..>4154)
                     /locus_tag="PLR93_05795"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNH26511.1"
                     /translation="LYPRIDYYYDSQQNMTIPGQHATLRDDLYILLVDWQPVSSEGAT
                     FKIYVNPLVNWLWIGSLTFLFGLIFAAWPDRDPADEPLRRKVSAGNRQTSAAD"
BASE COUNT          987 a          965 c         1122 g         1080 t
ORIGIN      
        1 actttggcag attcatttgg cagggagtat ccagctactt tgacattgcc caagctaggg
       61 cgggagagtg aggcgagaat ccgcagaaag gtggttttgc cggctccatt gggtccgagc
      121 aatgcgacga actcaccggg ttgtacagaa aaatccaacc ctttgagaat ggtcttcaat
      181 ccaaaacgtt tgacaagttt tttaacttcg atcatgagag acctgtgatg attgcaacgt
      241 gaaacgcgat gtaaattcgc gtttcacgtt gcatgtctta gcctttcttt ttcaacgaac
      301 ttgttaattc ggcgcggcgc tttttgtagg cttcgtcgct gatcttgccg gcgcgatgca
      361 ggtcgtcgag gacgatgatg gcatccataa tggattcggt gtcatcgaat tggtcatcat
      421 cttcttcatc gtcttcggct tcttcagatt cttcggcggc tttcttgcgg tcgcgcaagt
      481 acatgaagac accggcgagg atgagcgtaa taccaaaggc acccacaccg atcaatacgt
      541 actgattttg ggtgaggtct gcagtggcag aggtttcaga aggatcgcct ttgagcgtga
      601 agtctaatgt agagttggcg ctcacgtttg tgaatgaata ggtttgatat tcatagttct
      661 tgccgcttga gtcttgaagc ggaaccatgc cttgatctgt gagttggtcg ccttgtgcag
      721 tgacgccatt gggcaggaag acttttccac tgccaacggg aagcaagaac tcttgagaga
      781 aatcaacttt gtttgtgcgt ggcaggctgg agaaagcgat caaaccatag gtttggtcgc
      841 tgggctgaat gtagaagcca tcttgttctt tggtggcgag caaggttgcg ccatcttgtg
      901 tctgttggaa gccaagcagt ttggcattgg caggtgcttt gatgaacggg atatcttgat
      961 tgttgttttc gagctgtact ttgatgttct tatcgccagt gttgcgcaag agatagacca
     1021 caaacgcgct aatgctttcg gggtcggtgt aatcaaagtg gactgcaatg ctgtcgatca
     1081 caaggtcggt caattcggtg gtggtgtcgt agacggtgat gtcgggcaga gcgagctcgg
     1141 tagcgccggc gggcacaaca gcgaattcag attgataggt aatgccgtct acatccaact
     1201 ctgcgagata gatttgccct tcaagcagtt ctacgtcgaa ggaatatgaa ccatcggggt
     1261 tgacggtgcc ttgcacgttg agtacttcgg tcggacctgc gcttgggtca ctgccgtggt
     1321 cgaagccgcg caaggtgatc tgctggtctg cagaaaggtc tgcgcttgaa ttattgatga
     1381 tgaaaccctt aaccatgctg gcaactgcag cttctgtcgc tgctggtgta ccttcagctt
     1441 ggggtgtgcc atcagcaggt gtgctatcgg tggatggagt gctttcagcg gcggcgggag
     1501 tttcggtggc tgcagcagct accggcgcgg aaggaaccgc aaacgtcaac gtatggatgt
     1561 aggctgcaac ggcgtacgct tcttcgtcgc tgaaattgct tccaaaggct gggaagtttc
     1621 cattgccttc acgtacgaac ttgacgaggt catcttctga tagcgccgac atcatttgta
     1681 agttggtgaa tgcatcacca caatcggcac aattttcttc gaagagtttc ttgccgagtt
     1741 cgagttgttc gtttgtggtg tgaagtgtga gagcatacgc caccacatcc caacgttcct
     1801 ggtcggtgag gctgttgaag ggcggcatga agcgttcaat gcgtccctgg gttactacgg
     1861 cataccaact tgaaagtgtg gcttcatttg caaattcagg caaaccgatc ggaatgacgg
     1921 agacaggcag gtctttgctc tgttcgccat cgcccaagcc tttttctccg tggcacgctg
     1981 cgcatttctc agtaaagatg gctttgccat tttcgacatc gggagcttta tctggatata
     2041 gcggacccaa tgtgggtgga ggtgtggggg atacataccc aggcggcgga gtaacatctg
     2101 ccgcgagagt catattacag gcagagagca agagggtacc tattgctaaa agaaagaggt
     2161 gacgtaattt catgaattcc tcggtttgcg gattatttca aagactttcc gcaagatggg
     2221 cagaagttat cggcagtaag cacgggtttt ccgcacgaag ggcagaaccc ggcagatttg
     2281 cttttgtgtt gtttgcggcg ggcagagatc attgcttcta tatcttcatc gctcatatca
     2341 gaagcggtgg aggaagaatc tgcgcgtttg gcggcagccg ctttttcaat gcgggaggcg
     2401 gcatcgcgag cagaagaaag gatcggctcg agttcatcga gtttgcgtaa gatatcagag
     2461 cctttcttta ataactcagc gcgttgttcg gggtaatctt cagcaggaat tttgccgagc
     2521 ttgaaatcaa aatccaattc ttgcaacgag ttgatgacgc ggtcgcgttc ggctttcaag
     2581 gctgagatct catgcatttc gtccgttgaa agtttgcgcg aacgatacat gaaaggtgca
     2641 tatagataca ttcctactgc gacgagaacc gcgaggatga gaaagattga acctagttcc
     2701 atgagaaccc cctactctgc acttgcaata aaagcttgaa tttcagaaac cgatgcgaag
     2761 ggaccgatct tgatctggcg caagacaccg tcgcggtcga tcaaataggt ttcaggtacg
     2821 cccatctgac gattcaaaat gctggagatg tttgattgaa gatctggcgc attggggaat
     2881 gtaataccaa actttttcaa atagccaaac gcaccttcgg gcgtatccac ataatccacg
     2941 ccgatgaaaa cgacatcacc ttggtcttta tataactgcc aagcttgttc aagttcagga
     3001 gcttcctgtt cgcagggctt gcaccatgaa gcccaaatgt tgatgagcac aaccttgccg
     3061 cgcaaatcag aaagcttcat ctcaggcgta ttattgtgtt cgtagccttc gtaataaacc
     3121 aacttgaaat ctggcacggt atcgcccact tctgccatgg ggtggctggc gcggttcaaa
     3181 ccgaaaccca ccaatgcgag caaacccgca aggaaaaccc aaatggtgac ttgcacccac
     3241 aaggggacgc tctttctggc tggcgctgtt gtttctgtca ttgcattctc cgtaaaagac
     3301 cgattggtcc ctgttatttt ctgttcttca attcttcttc aagacgctta atgtattcat
     3361 cgtcactgga tgacgtgccc gcctcttctg cttgacctga acctgcctca gcggacttgg
     3421 gcttggtcca cgctcgaaac gcattgaaga ggaagacggc tcccacaagg atcaatactg
     3481 gtggaatgat atacaccaac cagttgaacc cagcccgtgg cggctcagag agcacacgag
     3541 caccgtagtt tgctacaaaa taatctttga tctgttgctc actcttgcct tctgccaaca
     3601 tctggcggat caactcgcgc cattgatgac aagcttcggt tgggcataca tccaacggcg
     3661 tattttcaca taccgggcaa tacaattgct tggcaattgc attcacttca tcatcagtag
     3721 gggttttgtc ttgggcaaat gccaccgccg tgaagagacc agaggcaacc agcataaggg
     3781 taagaataaa gaggaatttc ttcattgaag ttctccgaga tcggtcgaaa gacatttacc
     3841 ctcgaccgat gactcttttt agtcggctgc actcgtttgg cgattgcccg cactcacctt
     3901 acggcgtaga ggctcatcag ccgggtcacg gtcgggccag gcggcaaaga tgagaccgaa
     3961 taagaaggtc aggctaccaa tccacaacca gttcaccaac gggttgacgt atatcttgaa
     4021 ggtggcaccc tcgctcgaaa ccggctgcca atccacgagc aggatgtata agtcatcacg
     4081 caaagtggca tgctgacctg ggatggtcat gttctgctgt gagtcgtagt aatagtcaat
     4141 gcgcggatag agtt
//
LOCUS       DAOQFT010000178         4141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11692_length_4141_cov_5.378120, whole genome shotgun sequence.
ACCESSION   DAOQFT010000178 DAOQFT010000000
VERSION     DAOQFT010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11692_length_4141_cov_5.378120"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1038
                     /locus_tag="PLR93_05800"
     CDS             139..1038
                     /locus_tag="PLR93_05800"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26512.1"
                     /translation="MTIVYRSGTLEDSFAVFQVFLKSIMDYGNRMNVMAITGGNDPAK
                     LNSLWESRKPLFDFLAEHASQFWLAERDGEILGYARSIEHDGHQELTEFFVAPDQQSA
                     GVGSGLLSRAFADTGAKYRTIIATLDERALFNYMRMGVYGRIMLKYFNRKAEKVSVET
                     DLRIEPLNLEVHLDDLHRIDRDLLSHTRAEIHRWLAGRREGFVYKRNGEVVGYGYVGG
                     SHGPFAVLNADDFPAILAHAESRAAEKDEDFGVSVPLVNQQAVDHFVRRKYQVDSFTT
                     VLMSNVAFGKFENYLTFAPEFFL"
     gene            1035..1412
                     /locus_tag="PLR93_05805"
     CDS             1035..1412
                     /locus_tag="PLR93_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26513.1"
                     /translation="MKIKSIPILLSAFVLIFSSLACWRTNRPELVFAPADLPEAQIGV
                     PYDVEIEVSQNETPVYIMSISEGVLPRGLVFEFDENAHEDKARIYGTPEESGTFTFKV
                     FAGCLGTNVSGQTGEWEYTLVVK"
     gene            complement(1445..2719)
                     /locus_tag="PLR93_05810"
     CDS             complement(1445..2719)
                     /locus_tag="PLR93_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH26514.1"
                     /translation="MKIAILGAGYGGMVAAWDLRKAGHDVTIFEAANYAGGLASGFKE
                     PHWDWSVEKFYHHWFQSDSEMLGLIRELGLEDKVIFPRPLTVMLYKNKWYPFDSILNA
                     LRFPGLGFGLNKIRFGFVGLFLRITKNWQALEKVTAHEWMLKYAGKQVYEQMWQPLLI
                     GKFASYYKDVNMAWLWARMHARTTRLGTFEGGFQKFADLFADKLREKGVEIRLGTSIK
                     SIKKDQASGSLSVDGESFDKVLVTTSPSLMAKLCPELPESYLKGLLELKSMGAVVMTL
                     SLKNSLSKEGYYWFNVPKEEGYPFLALVEHTNFVSKEHFGGDHIVYAGDYLELGHEYF
                     EMTDEQLLEKFIPAFEKFNPEFKRDWVKKIWVHKTNYAQPVPLVGHSKNIPAIQTPIE
                     GLYFASMSQVYPWDRGTNFAVEIGRRAARMMK"
     gene            complement(2735..3346)
                     /locus_tag="PLR93_05815"
     CDS             complement(2735..3346)
                     /locus_tag="PLR93_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26515.1"
                     /translation="MQPQLRKKRVLIADTTQETRRNTRVMLSTVKGVEVVAIALDGNQ
                     AVEMAKLHKPDIVLLDINMSKLNGLNAYKQILKVNPTAACILVSAENHPETVEAAKEM
                     GIQNFLVKPFTEHELEHAMQDVIQRLDGKLNNQRTIEVRKLADEYVRTKRTDEEAVQV
                     FEEAIQLPDSELVWMQTLAMIYVVRKRWGKLKVLAEQLELRTN"
     gene            complement(3440..>4141)
                     /locus_tag="PLR93_05820"
     CDS             complement(3440..>4141)
                     /locus_tag="PLR93_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNH26516.1"
                     /translation="DVPVSASLATIIVERIFDGVVMLAFVFVNLSELAKLTGASGFVG
                     NIQQVAMIGTEVFLGALLVFLLAAMFPQFTSRVGTWVIERLTPQRLHEKLTGIMNKFL
                     DGLASLRSPFNVLMVFFTSVVIWLLETAKYWFVMHAFNFTVSFFALMLMNGIVNLATT
                     IPSAPGYIGTFDAPGIAVLTAYGVDQATAAGYTLVLHVALWVPITALGAYYLAREGIK
                     WSDTLREEAETPNTP"
BASE COUNT         1026 a         1021 c         1040 g         1054 t
ORIGIN      
        1 gggtttgaca aagtccgggt tcatactgcc attgtagcgt agacttggaa tcccgaagtt
       61 ctatttctgg atagttcagt ttgattctca ttaaaaactt gaatgctata atgcctgaac
      121 tttataaaag gaatcaaaat gaccattgtt tatcgctctg gaacccttga agactctttt
      181 gcagtctttc aagtttttct caaatccatc atggattatg gcaaccgcat gaacgtgatg
      241 gcgatcaccg gtgggaacga cccggccaag ctcaattcac tttgggagag tcgcaaacca
      301 ttgttcgatt tcctggcaga gcatgcctcc cagttttggc tggcggagcg tgatggtgag
      361 atattgggct atgcccgctc gattgagcat gacgggcatc aggagttgac cgaattcttc
      421 gtagcgccgg accagcaatc ggcgggggtt ggaagcgggt tgttatcccg cgcgtttgca
      481 gataccggcg cgaagtatcg caccatcatc gccacattgg acgaacgcgc tttgttcaat
      541 tacatgcgca tgggtgtata tggccggatc atgctcaagt atttcaaccg caaagcggaa
      601 aaggtgagcg tggaaactga cttgcgtatc gagccgttga atttggaagt tcatctggat
      661 gacctgcatc gaattgaccg tgaccttctc agtcatactc gtgcggagat tcatcgttgg
      721 ctggcgggca ggcgcgaggg atttgtatat aaacgaaatg gggaagtggt cggttacggt
      781 tatgtgggtg ggagtcatgg cccgtttgcg gttttgaacg cggatgattt ccctgccatc
      841 cttgcccatg ccgaaagccg cgcggcggag aaggacgaag attttggtgt atctgtgccg
      901 ctggtcaatc aacaggctgt cgatcatttc gtcaggcgaa aatatcaggt agactcgttt
      961 acgacggtgc tgatgagcaa cgtcgcgttt ggaaagttcg agaattattt gacgttcgcg
     1021 ccggagttct ttctatgaaa ataaaaagca ttcccatctt gctttcggcg ttcgttctga
     1081 tattttcctc gcttgcctgc tggagaacga acagacccga actggttttc gccccagcgg
     1141 acctacccga agctcagatc ggcgtcccct acgatgtaga gatcgaggtt tcgcagaacg
     1201 agacgcccgt ctacataatg tccatttccg aaggcgtcct tccgcgggga ttggtctttg
     1261 aattcgacga gaacgcacac gaagacaagg cgcggattta tggcacgcct gaagaatcag
     1321 gaacattcac gttcaaggtt ttcgcgggat gcctcggaac caatgtcagc gggcagacgg
     1381 gggaatggga atatacgctg gtggtgaaat aaaaagatgc cctctcgcat gagagggcat
     1441 cttgctactt catcatcctc gccgcacgtc tgccgatctc caccgcaaaa ttcgtaccac
     1501 gatcccacgg gtagacttgg ctcatcgaag cgaagtacaa gccttcaatg ggtgtttgaa
     1561 tagcaggaat atttttggaa tgtccaacca aaggcacggg ttgagcgtaa ttggttttgt
     1621 gaacccaaat cttcttgacc caatcgcgtt taaattcagg gttgaacttc tcaaaggctg
     1681 gaataaattt ttccagcaat tgttcatcag tcatctcgaa atattcgtga cccaattcaa
     1741 ggtaatcacc ggcataaaca atatggtcgc cgccgaaatg ctccttcgaa acaaagttgg
     1801 tgtgctccac cagtgcaagg aaaggatagc cctcttcctt aggcacgttg aaccagtaat
     1861 agccttcttt cgaaagcgaa tttttcaaag aaagagtcat caccactgcg cccatcgact
     1921 tcaactccag caaacccttc aaataagatt cgggcaactc ggggcataac tttgccatca
     1981 aactgggtga cgtggtgacg aggactttgt cgaaggattc accgtccacg ctcaaactac
     2041 ccgacgcttg atctttcttt attgacttga tgcttgttcc aagccgaatc tccactccct
     2101 tttcgcgcag tttgtctgca aagaggtcag cgaatttttg aaagccgcct tcgaaggtgc
     2161 cgagccgagt ggtgcgggca tgcatacgcg cccataacca cgccatgttc acatctttgt
     2221 agtacgaagc gaacttgcca atgagtaaag gctgccacat ctgttcgtag acttgtttgc
     2281 cagcatactt caacatccat tcgtgggcgg tcactttttc cagcgcctgc cagttcttgg
     2341 tgatgcgtag aaaaagcccg acgaagccaa atctaatttt gttcagacca aatccaagtc
     2401 cgggaaaacg aagcgcgtta agaattgagt cgaagggata ccatttgttc ttatacagca
     2461 tcacggtcaa cgggcgcggg aagatgacct tatcctccaa gcctagctca cgaatcagcc
     2521 ccaacatttc gctatcagac tgaaaccagt ggtggtagaa cttttccacc gaccaatccc
     2581 aatgcggttc tttgaacccg ctggcaagac cgcctgcata atttgctgct tcaaaaatgg
     2641 tgacgtcgtg acctgcttta cgtaagtccc aagcggcgac catgccgccg taccctgctc
     2701 caagaattgc gattttcaag agagttctcc aaatttaatt tgttctgagt tccaattgct
     2761 ccgcaaggac ctttaacttg ccccagcgtt tgcgtacaac atagatcata gcaagtgttt
     2821 gcatccaaac caattcactg tcaggaagtt gaatggcttc ttcaaaaact tgcacagctt
     2881 cttcgtccgt tcgttttgtg cggacgtatt catctgccaa ctttctgact tcgatagtgc
     2941 gttgattgtt aagcttgcca tccagccgtt ggataacatc ttgcatggca tgttccagtt
     3001 catgttctgt aaacggtttg acgaggaaat tctgaatgcc catttccttg gcggcttcta
     3061 cagtttcagg atgattttca gccgagacga ggatgcacgc cgcagttggg ttgaccttta
     3121 gaatctgttt gtaagcattc aacccattga gtttagacat gttaatgtcg agcaatacga
     3181 tatctggttt gtgtagcttt gccatttcaa cggcttgatt tccgtcaagt gcaatggcaa
     3241 ccacctcaac acctttgaca gtagaaagca ttacacgcgt gttacggcgg gtttcttgtg
     3301 tagtatctgc tataaggact cgtttttttc taagttgggg ctgcatatcg tcctgccttc
     3361 ttaatagggc aattggttca acctattaag tataaccgag gcgggcatcc gaagatgccc
     3421 gcctgtcaga gctgtaaatt tatggggtgt tgggtgtttc tgcttcctcg cgaagcgtgt
     3481 cgctccactt gatgccctcg cgagccaggt aatacgcccc caatgccgta atgggaaccc
     3541 acaacgccac atgcagcacg agggtatagc ctgccgccgt ggcttggtcc acgccgtagg
     3601 cggtgaggac ggcgatgccg ggcgcatcga aagtgccgat atagccgggt gccgaaggaa
     3661 tggtggtggc gaggttgacg atgccgttca tcagcatcag tgcaaaaaat gagactgtga
     3721 agttaaatgc gtgcatcacg aaccaatact tggcggtttc caaaagccaa atgaccaccg
     3781 aagtgaagaa gaccatcaac acattgaagg gcgaacgaag cgaagcaagc ccatccagaa
     3841 atttattcat gataccggtc agcttttcgt gcaaacgttg cggggtcagg cgttcgatca
     3901 cccacgtacc gactcggctg gtgaactgtg ggaacatggc tgccagcaaa aagaccagca
     3961 atgcgccgag gaagacctcg gttccaatca tcgccacttg ttggatgttc cccacaaagc
     4021 cagatgcccc tgtgagtttt gccaactcag acaagttcac gaacacgaac gccaacatca
     4081 ctacaccgtc gaagatgcgc tccacgatga tggtcgccag cgaagcagag acggggacat
     4141 c
//
LOCUS       DAOQFT010000179         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11781_length_4125_cov_5.641769, whole genome shotgun sequence.
ACCESSION   DAOQFT010000179 DAOQFT010000000
VERSION     DAOQFT010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11781_length_4125_cov_5.641769"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..448
                     /locus_tag="PLR93_05825"
     CDS             248..448
                     /locus_tag="PLR93_05825"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNH26517.1"
                     /translation="MKRNLSDVDRIIRVVLAAIFAYLYFGGIVTGTFGIVLVVLAVVF
                     ALTAVVAFCPLYAPFKLSTYKK"
     gene            501..1001
                     /gene="trxA"
                     /locus_tag="PLR93_05830"
     CDS             501..1001
                     /gene="trxA"
                     /locus_tag="PLR93_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNH26518.1"
                     /translation="MNKQEFQEKINSSEKPLVVDFWATWCAPCMMTKPILEKLGKEYA
                     EDVEFLPINADESRDVLEDYRILGIPTVLAFKGGKEVARVTGAQNEAGYRAIFESMAK
                     GVKVKIPMTQIDRMLRLGAGGLLVMYGLPDRNWIVVAIGAVVAFLGIYDRCPIWNALT
                     KPFQRK"
     gene            1084..1707
                     /gene="udk"
                     /locus_tag="PLR93_05835"
     CDS             1084..1707
                     /gene="udk"
                     /locus_tag="PLR93_05835"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNH26519.1"
                     /translation="MMHKIPLIIGVAGGSGSGKTTVAQELLRRVGADRIAYLQHDSYY
                     KDLRMLPSTQHSEINFDHPDTLETELLIKHIIDLREFKAVQIPIYDFSTDTRKTETIT
                     VEPRNVILVEGILLYVEPELRKLFDVKLFVDADADIRFIRRLHRDITERGRSTESVIE
                     QYYATVRPMHLEFVEPSKRYADVIIPEGGHNVAALDMVTARIELLLK"
     gene            1831..2253
                     /locus_tag="PLR93_05840"
     CDS             1831..2253
                     /locus_tag="PLR93_05840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH26520.1"
                     /translation="MVIELFADKAPVTVNNFVFLSREGYYDGVIFHRVISNFMAQGGD
                     PTGTGRGGPGYKFQDEFHPSLKHSKRGILSMANAGPGTNGSQFFITHGPTPHLDNRHT
                     VFGEVVEGEDVLMSIPERDPMKLNAPAVKIIRVTIEEI"
     gene            2231..2803
                     /locus_tag="PLR93_05845"
     CDS             2231..2803
                     /locus_tag="PLR93_05845"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNH26521.1"
                     /translation="MSPSKKSKPENKVVTAILRILAVLAVIGITVYVYSIRNHVEEFA
                     NYGYFGAFLIMLIANATVILPAPGVAIVFAMGSMLNPLGVALAAGVGGMLGEMTGYLA
                     GYSGQAAVENTTVYNRIVPYVQKYGAWVILVMSAIPNPFFDLAGIAAGIAKIPVWQFM
                     LACWVGQTIKMSMFAFAGAYSIDWIARLYK"
     gene            2861..>4125
                     /locus_tag="PLR93_05850"
     CDS             2861..>4125
                     /locus_tag="PLR93_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH26522.1"
                     /translation="MSNYDKVDAFIDKNLSQSLDELKKYVAQPSISAQNLGLKECAQL
                     VKEMLEKRGFTAEIMATEGAPVVFAERKGNVDKTILIYNHYDVQPPEPLELWDSPPFE
                     PEIRDGKMYGRGVSDDKGHLTSRLHAIDAILAEDGELPCNIKFIIEGEEETASVHLHE
                     FISNNVERLKADACIWEFGSVDHRDRPQQYLGLRGICYVELSVTSLGTDVHSGIGGSI
                     FPNAAWRLTWALSTLKGVDERIRIPGFYDDVVQPSDRDREFMEKLPDLAEDYKTRFGV
                     QKFIKGLEGGMDLKLEEVFTPTCTICGLTSGYQGPGSKTVQPASASAKVDFRLVPNQM
                     PKDILKKLRAHLDAEGFSDVEIKFLGGEPAARTDPDHPFVKLVSETAEEVYDAKMDIV
                     PMIGGSGPNYPFVHELGLPVVCMGIGYPNT"
BASE COUNT         1105 a          923 c         1029 g         1068 t
ORIGIN      
        1 aagaagaaaa ccctattcac atcactcatc aggaaatcgc ttcagatctg ggaagttcca
       61 gagaagtgat tagccgctta ctcgaagatt tcatcagtga gggcagcgtc cgctcggggc
      121 gtggaatcat cgaagttcta gattttaagg cgttggaaga tcgttcgctt atgtgacata
      181 gtcacagaca ggttcgggag tttctcctat actacggtta acaataacca aaaggagaaa
      241 ctagaaaatg aaacgaaatt tgtctgatgt cgaccgaatt atccgcgtgg tgctggctgc
      301 gatttttgcc tacttgtact ttggtggtat cgttactgga acgttcggta ttgtcttggt
      361 cgtgcttgct gtcgtatttg ccctcaccgc tgtggttgca ttctgcccgt tgtatgcacc
      421 tttcaaactt agcacctaca aaaagtaatt caaaacattt gggagcgggc aatgttttgc
      481 ccgctcctat cctttaaatt atgaacaaac aagaatttca agagaaaatc aattcaagcg
      541 aaaaacccct cgtagtggat ttttgggcaa catggtgcgc tccatgcatg atgaccaaac
      601 ccattctaga aaaactcggc aaagaatatg ccgaggatgt ggaatttcta cccatcaatg
      661 cagatgagtc tcgggatgta ttggaagact atcgtattct cgggattccc actgtgctag
      721 catttaaggg tggaaaagaa gtagcacgag tcactggcgc tcagaacgaa gcgggctatc
      781 gtgccatctt tgaatccatg gcaaagggag tgaaagtaaa aatcccgatg acccaaattg
      841 accgtatgct ccgtttgggt gctggtggct tgctggttat gtatggtttg cctgacagaa
      901 attggatcgt tgtagcgatt ggcgcggttg ttgctttttt gggcatttac gaccgttgcc
      961 caatttggaa tgcactgacg aaacccttcc agcgaaaata aagttcaatg gttaggaaac
     1021 aggctccggc ttcgtccgga gccgtttgca tcccaatgag agtggcgggt ataatctcgt
     1081 catatgatgc ataaaatccc tttgattatt ggagttgcgg gcgggtcagg ttcgggcaaa
     1141 acaacggttg cacaggaatt attacgaaga gtgggggcag atcgaattgc ttatcttcaa
     1201 cacgattcat attacaaaga tttgcgaatg ttgccatcta ctcaacattc tgaaatcaat
     1261 tttgatcatc cagacacgct tgagactgag ttattaatca aacatattat tgacttgcgc
     1321 gagttcaaag ccgtgcagat tccgatttat gatttttcaa cggacacacg taagacagag
     1381 acaatcaccg tagaaccacg aaatgttatt ttagtggaag ggattctttt gtatgtggag
     1441 cctgaactac ggaagctttt tgatgtaaaa ctttttgtag atgcggatgc agacattcgc
     1501 ttcattcgcc gcctacacag agacatcacc gaacgcggac gatccactga atctgttatc
     1561 gaacaatatt atgcaactgt gcgcccaatg catttggaat ttgtagaacc ttcaaagcgc
     1621 tatgcagatg tcattatccc agagggcggg cataacgttg ccgctctgga tatggttaca
     1681 gcaagaatcg aattgctgct caaataaacc caattttata ttcatctact caaggagaga
     1741 cgtatggcaa aacaatggag cacccccccg gcaatgcaga tcgaccccaa gaaaaaatac
     1801 aaagctcaca tggaaacaga taacggtacg atggtgatcg aactgttcgc tgataaggcg
     1861 cctgtaaccg tgaataactt tgtgtttctt tcgcgcgaag gctattatga cggtgtgatc
     1921 tttcaccgtg tgatctcaaa ctttatggca caaggcggcg acccgaccgg taccggacgc
     1981 ggcggacccg gctataaatt tcaggatgaa ttccacccaa gcctgaagca tagcaagcgc
     2041 ggcatccttt ccatggcaaa tgcaggaccc ggcacaaacg gctcgcaatt cttcattact
     2101 cacggaccca ccccgcactt ggataaccgt cacaccgtgt tcggtgaagt ggtggaaggc
     2161 gaagatgtgt tgatgtccat tcctgaacgc gacccgatga agctcaacgc cccggctgta
     2221 aagatcatcc gtgtcaccat cgaagaaatc taagcctgaa aataaagtgg tcaccgccat
     2281 actgcgcatt ctggcagtac tggcggtgat tggcattacg gtctatgtgt acagtatccg
     2341 taatcacgta gaggaatttg ccaattatgg ttattttggc gcgttcctca tcatgttgat
     2401 tgcaaatgcg accgtgattc taccggctcc gggtgtggcg attgttttcg ccatgggcag
     2461 tatgctgaat ccacttgggg ttgcactggc tgcaggggtc ggcggtatgt taggtgaaat
     2521 gacaggctat ttggcaggct atagcgggca agccgctgta gaaaatacaa cggtctacaa
     2581 ccgcattgtg ccatatgtgc aaaagtatgg tgcctgggtc attctcgtta tgtcggcaat
     2641 tccaaatcca ttttttgact tggctggcat tgcagcaggc attgcgaaaa ttcccgtctg
     2701 gcagtttatg ttggcatgct gggttgggca aacaatcaaa atgtctatgt ttgcttttgc
     2761 cggagcctat tccattgact ggatcgcccg tttatataaa taaccaaaac ctgacaggtg
     2821 ctctgtcagg tttattttta tctcagcaaa ggagtttcag atgagcaatt atgacaaagt
     2881 cgatgccttc atcgataaaa atctaagcca gagtttagat gaactcaaaa agtatgttgc
     2941 ccaaccaagc atcagcgctc aaaacttggg actgaaggaa tgtgcccaac ttgtaaaaga
     3001 aatgctggaa aagcgggggt tcacagctga gatcatggca acagagggag cgcctgttgt
     3061 ctttgcagaa cgcaaaggca acgtggataa aaccatcctt atctacaatc attacgacgt
     3121 tcagccgcca gagccattgg aattatggga tagcccgcct tttgagccag agattcggga
     3181 tggcaagatg tatgggcgcg gtgtgagcga tgataaaggt catctcacat cacgcttgca
     3241 tgcgatcgat gcgatccttg cagaagatgg cgaattacct tgcaatatca agttcatcat
     3301 tgaaggtgaa gaagaaactg ccagtgtgca cttgcacgag tttatctcca ataatgtaga
     3361 acgactcaaa gccgatgcct gcatttggga atttggcagt gttgatcatc gcgaccgccc
     3421 acagcagtat ttggggctgc gtggaatttg ctatgtggaa ttatcggtca cctcgctggg
     3481 cacagacgtt cactctggca ttggtggcag catcttcccc aacgccgcat ggagattgac
     3541 ctgggcattg agcaccctta aaggtgtgga tgaaagaatt cgcatccccg gtttttatga
     3601 tgatgtggtg caaccttcag accgtgaccg tgagtttatg gaaaaactcc cagacctggc
     3661 agaggattat aaaacccgct ttggagtgca gaaattcatc aaaggcttgg aaggcggcat
     3721 ggatttgaaa cttgaagaag ttttcacgcc tacttgcacc atttgcggtc tcaccagcgg
     3781 ataccaaggt cccggctcga aaaccgttca gcctgcctct gcctctgcaa aagtggactt
     3841 ccgccttgtg ccgaatcaaa tgccgaaaga catcctcaaa aaactgcgcg cccatttgga
     3901 tgccgaaggc ttcagcgacg tcgaaattaa attcttgggt ggcgagcctg cggcgcggac
     3961 agatccggat catcctttcg tcaaactggt cagtgaaact gcagaagaag tctacgatgc
     4021 gaagatggac attgtcccca tgatcggcgg ttctggacct aattatccgt tcgtgcatga
     4081 acttggattg cccgttgtgt gcatgggaat tgggtacccg aatac
//
LOCUS       DAOQFT010000180         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11782_length_4125_cov_4.632187, whole genome shotgun sequence.
ACCESSION   DAOQFT010000180 DAOQFT010000000
VERSION     DAOQFT010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11782_length_4125_cov_4.632187"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..775)
                     /locus_tag="PLR93_05855"
     CDS             complement(101..775)
                     /locus_tag="PLR93_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNH26523.1"
                     /translation="MSTDSHRQLRNVVADKMRAAILDGQYKPGEWLRQERLAQELGVS
                     QMPVREALNELVNEGLIEHVPYRGARVIQFSIEDVIDLYEHRAFLEGRAAYFAAQIIT
                     DRDLTALKKIQVGMEENDAPEAVLKYRDLNRNFHKTIFTISKHKYLIRTLNQMWAAFP
                     TMLIGNFAATASHPLPARDDTDRQEHQAIMDALFAHDGTSAEQAMRNHILATREQLVS
                     FLKANQ"
     gene            complement(785..1792)
                     /locus_tag="PLR93_05860"
     CDS             complement(785..1792)
                     /locus_tag="PLR93_05860"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNH26524.1"
                     /translation="MKLVFPFFRFTTLFIILVSLISPSPVFAAKKHYEYYVVGNSNNA
                     TSPTQAGTVLMGGGTDVDAAFQWMINKSGGGDFVVIRASGTDAYNPYIYGLGTVDSVE
                     TLIITSRTGASDPFVLDKINNAEALFIAGGDQADYVNYWKGTPVEDAIHNLIARNVPI
                     GGTSAGLAILGEFSFSAANGTVDSSAALGNPFGRRIALERDFLTVPYLSSLITDSHFV
                     TRDRMGRLVTFLARIVNDGWAPQAKGLGIDEATAVVVEADGSASILGNGAAYFLQTPG
                     TPEVCLPKTDLTYRNVSVYRISGSATFNFATWTGSGGTAYTITAENGTVTSSQPGGSI
                     Y"
     gene            complement(2117..2842)
                     /locus_tag="PLR93_05865"
     CDS             complement(2117..2842)
                     /locus_tag="PLR93_05865"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNH26525.1"
                     /translation="MRKPLFLAVMILIMLACAVPGAPAVAPLPTFDPNSLQTFIVQTA
                     EAAQTQTALVMPTFTKTPTTTFTPSITPSSTPTFIFKLSTPTPIPTYTIPPTIGTLKS
                     PAAPSGSSDEENSTGDKPKKDEDPKKMSGRDWTCVWYGLTPPRNTVFKPKTHFTVQWM
                     LFNSGNQSWPRTGVDFVYTGGFRHEETKIQDFVQSVSPGKEIWVRASFIAPKAAGDYA
                     TYFTLMVGKRKFCPVAYFFTVEE"
     gene            complement(2855..>4125)
                     /locus_tag="PLR93_05870"
     CDS             complement(2855..>4125)
                     /locus_tag="PLR93_05870"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNH26526.1"
                     /translation="RIIKFPPAVVEEAIKSAPKTIDVYDRRGEHKLKLGEDRLRFGVG
                     VTALFYQNPVDETLDIFKRENFRDLVRLASGLKHYDVISTVGIVRDVKEELGDMYGSL
                     EHISNTTKPLVLLVSDEDRFPDVLDMFEHLNGKEVGDKPFVIPYFNPVSPLVMNAGTV
                     DKMKVAIERGLPIIFSNYSMAGASTPLTPAGTLTLLMAELLAGLVISQTIKKGAPILL
                     GMLPVYFDMRTMLNFYDPQSILISVACSEMMKFYGIPHCSTSGSGTGWGMDLIAADTY
                     WMNTLALLLSNGHLAPFIGDSLGSKSISPTTFVHAHEIIDQALRLHNGFQLDDANSAV
                     DEIFKVGPGKSFLNQPSTLKNYKNGYYVSGVYPRYSMEKWMDAGAPPARQVLRQKTQD
                     LMASAPVPNEYDEFIAKGEEFIRKRFHDIL"
BASE COUNT         1117 a          937 c          970 g         1101 t
ORIGIN      
        1 accacaatgt cggctttttc ccctcctgca aggcggataa actctttcga aacgactggt
       61 ttcttgtcgt tttcgtttcc accaatcggc attaacagcg tcattgattc gcctttaaga
      121 atgaaaccaa ctgctcacga gtagcgagaa tatgatttcg catggcttgt tccgcagaag
      181 tcccgtcatg tgcaaataaa gcatccataa tcgcttgatg ctcctgccga tctgtatcat
      241 cgcgggcagg caaagggtgc gatgctgtag ctgcaaagtt gccaatcaac atcgttggga
      301 aagcagccca catttgattt agagtgcgga ttaagtattt atgtttacta atcgtaaaaa
      361 tagtcttgtg aaaatttcgg ttaaggtcac gatacttcaa tacagcttct ggagcatcat
      421 tctcttccat tccaacctgt atctttttta gagccgttaa gtcacggtct gtaataatct
      481 gtgctgcgaa atatgctgca cgcccttcaa gaaaggcacg atgttcatac aggtcaatta
      541 catcttcaat agaaaactga atgacacgcg cgcctcgata agggacatgt tcgatcagcc
      601 cttcattgac aagctcattt agagcttctc gcacaggcat ttggcttact cccaactcct
      661 gtgcgaggcg ttcctgacgc agccattctc cgggtttgta ttgcccatca agaatggctg
      721 cacgcatttt atctgcgaca acgttccgta gttgtctgtg agaatctgta ctcatagctc
      781 cgtcttagta aatggatcca ccgggttggg aagatgtcac agtgccattt tcagcagtga
      841 tcgtgtaagc tgttccgccg ctgcctgtcc atgttgcgaa gttgaatgtg gctgagccgc
      901 taatgcgata aacagaaaca ttacgatagg ttaggtcggt tttaggtagg caaacttcag
      961 gcgtgccagg agtttgaagg aagtacgcag ctccgtttcc aagaatagag gcgctcccgt
     1021 cagcttcaac aactacggca gttgcttcat cgatgcccaa cccttttgcc tgcggtgccc
     1081 atccatcgtt gacgatacga gcaaggaatg taacgagtcg acccatacgg tcacgggtaa
     1141 caaagtgact atccgtgata agactgctca aataagggac agtaagaaaa tcacgctcga
     1201 gagcaatacg acgaccaaag gggttgccta aagccgcgga agagtctact gtgccatttg
     1261 ctgctgagaa agaaaactct cccaaaattg caagcccggc acttgtgcct cctataggca
     1321 cgttccgtgc aatcagatta tgaattgcat cttccactgg agtccctttc cagtagttaa
     1381 cataatcagc ctggtcgcct cctgcaataa acagagcttc agcattattg atcttatcca
     1441 atacaaaggg gtcacttgcg ccagtacgtg atgtaataat gagagtctct acagagtcaa
     1501 ccgtgcctaa accataaata taaggattat aggcatctgt acctgaagcg cgaatgacaa
     1561 caaaatctcc cccacctgat ttattgatca tccattgaaa tgctgcatca acatcggtgc
     1621 caccgcccat gaggaccgtt ccggcttgag taggtgaagt ggcattgttt gagttaccaa
     1681 caacatagta ttcataatgt ttttttgctg cgaaaacggg gctaggggat ataagcgaca
     1741 ccaagatgat aaacaaggtg gtgaatcgaa aaaatggaaa tacaagcttc attttatgct
     1801 cctttggcat agcacggtat tgaaggtggg tatttctaaa ttagatataa tatctaggtt
     1861 taggatagcg tttattggtt gatgtgtcaa tactttttaa gaggaataat ttgatattta
     1921 atgactatgc gcaatttact atattacaaa ttatgctctt aataattagg tgcgacttat
     1981 tgcaaaaacc tacagctaat tatgctggca taaaaagaga ccctatacta tagggtctct
     2041 tcccaaaaat attatcccct cccctattag aacttgtgag aaaatagtaa aaacaacgtg
     2101 cgtggctata cgcatttcat tcttcaactg taaagaaata tgctacaggg caaaactttc
     2161 ttttgccaac catcaatgta aagtatgtag cataatctcc ggctgctttg ggagcgataa
     2221 aagatgctcg tacccaaatc tctttgcctg ggctgacact ttgcacaaaa tcttgaattt
     2281 tcgtctcttc atggcggaac ccgccagtat aaacaaagtc aacgcctgta cgcggccaag
     2341 attgattgcc actattaaat aacatccact gaactgtaaa atgagtttta ggcttgaaca
     2401 ccgtatttct tgggggtgtc aatccatacc atacacaagt ccaatctctg ccagacattt
     2461 tctttggatc ttcgtctttc tttggcttat caccggtaga gttctcttca tcactactgc
     2521 ctgacggcgc agctggagat ttcaaggttc cgatcgtagg cggaatggta taagttggta
     2581 tcggtgttgg agtagaaagc ttaaaaataa aggttggagt agagcttggt gtaattgacg
     2641 gcgtaaatgt ggtggtggga gtttttgtaa aagtaggcat caccaaagcg gtttgagttt
     2701 gagccgcttc tgccgtttga acgataaagg tttgaaggga atttgggtca aatgttggca
     2761 aaggggcaac agctggcgca ccggggacag cacaagccaa cattattaaa atcatgacag
     2821 cgagaaaaag aggtttacgc acgggaactc ctacttaaag tatatcgtga aacctttttc
     2881 ggataaactc ttctcccttt gcaatgaact catcatattc gttaggtaca ggcgcggatg
     2941 ccattaaatc ctgggttttt tggcgcaata cctgacgggc aggcggcgca ccggcatcca
     3001 tccacttttc catgctgtag cgcggataga ctccacttac atagtatccg ttcttgtaat
     3061 ttttcaaggt agaaggttgg ttgaggaaac tcttgccggg acccactttg aaaatctcat
     3121 ccactgcgga gttggcatcg tctaactgaa agccgttgtg gaggcgtaag gcttggtcta
     3181 tgatctcgtg tgcgtggacg aaggtggttg gggagatcga cttcgagcca agagaatctc
     3241 cgataaacgg agccaggtgt ccgttagaga gaagaagcgc gagggtattc atccaatagg
     3301 tgtctgccgc aataaggtcc atgccccagc cggtgccact accagaggtg gagcaatgcg
     3361 gaatgccgta gaacttcatc atctcggaac aagcaacgct aatgagaata ctctgcgggt
     3421 catagaaatt gagcatggtg cgcatgtcaa aatagacagg caacatgcct agcaaaattg
     3481 gcgcgccttt tttgatagtt tgactaatga ctaaacctgc aagcaattct gccatcagga
     3541 gcgtgagcgt gcccgctggc gttaatggcg tggacgcacc cgccatgctg tagttggaaa
     3601 agatgatggg aagcccgcgt tcgatggcga ctttcatttt gtcgacggtg ccggcattca
     3661 tcacgagcgg actgaccgga ttgaaatacg gaatcacaaa gggcttatcg ccgacttcct
     3721 tgccattcaa atgctcgaac atatcgagca cgtctggaaa gcggtcttca tctgagacaa
     3781 gcaacaccaa tggcttggtt gtattggaga tgtgctccaa tgagccgtac atatcgccaa
     3841 gttcttcttt tacatcacgg acgatcccca ccgttgagat gacgtcataa tgtttgagcc
     3901 cagaagccaa gcgtacaagg tcgcgaaagt tctcgcgttt gaaaatatcc agcgtttcat
     3961 ccaccgggtt ttgatagaac agcgccgtca cccccacccc aaagcggagg cggtcttccc
     4021 ccaatttcag cttatgttcg ccgcgacggt cgtaaacatc tatcgtcttt ggcgcggact
     4081 tgatcgcttc ttcgacaact gcgggcggga atttgataat gcggt
//
LOCUS       DAOQFT010000181         4113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11826_length_4113_cov_6.577378, whole genome shotgun sequence.
ACCESSION   DAOQFT010000181 DAOQFT010000000
VERSION     DAOQFT010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11826_length_4113_cov_6.577378"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..2424)
                     /locus_tag="PLR93_05875"
     CDS             complement(43..2424)
                     /locus_tag="PLR93_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNH26527.1"
                     /translation="MKNKYLKFLFPIALILALTVSMIGTRSVLADDAPPTPTDEVPTE
                     TPPVEESSETQETLEQTVPEILEQLPAETEIVVLDENSEPLPLVSNEAANVLVTGDPQ
                     WCPAGVTPGTDTLGQCTVAHTSFNDLINDLQTNSATYYGSGTIYVAYDYAANTAGDSS
                     NDIIFDYGALDLTDLVVQGGWDFTQDKVVGTSTIDLKPGYSLEFWDWGGYGTPATLTL
                     KNLFITNSDGLFIGDDSDVTTADITLENVEVDSTEFGTYIETDGDIEITNSKFNNTAN
                     DNGLTIYSYGGDVTLEQVLARQNDGDGAYIEADGDITIKNSVFDNNDLSGAYIDTSCG
                     CGTGNVFVQSSTFIENNDRGLVVYAIGDITVDSIDAELNEVGGVELDNCGIFGGSPCF
                     NTNLVSITMTGTNTIKNNGGTPGSWFIGPASVGLWVTAADNISISGTTVTGNGAGDIG
                     GGAFVLAGNGNISISNSTFDDNCTTYQCDMGFGLFTLNDVGTVTIKNVTADGNGNGDG
                     STYNSDTGNGVLIISNGNIFVENSVFNGNCNTGTCSIGAGIQVATPGNVYFNFVTASN
                     NGTMLGGGANITVGGNVDVYCSFFANEAVGLAVDIPTGSVLTMRGVSFSGNTTDFVPP
                     SSGGTWTSDPNCPVNAFSGGGKGNTNLPLRIVNVGSGNGIDLDCQNYSGTKLVLENGD
                     NLVVPCPLGGSASLTKQDANGLPSSLPSGSTFRSGIVSVVTESGSVDGKLSQPVLVSF
                     IMPEGVDASALAILYWDGSQWTEVNGTFVLTENGKTYFAAFVNYTGTFVLVQR"
     gene            complement(2596..2997)
                     /locus_tag="PLR93_05880"
     CDS             complement(2596..2997)
                     /locus_tag="PLR93_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNH26528.1"
                     /translation="MKLTTRSEYALLALVYLARNEDEGFISIDTIATAQGIPPKFLEQ
                     LMLALKRAHFLRSAKGQKGGYQLAKRPDQISLAEVIRLFDGALAPTESVSENFYESTP
                     IEKEKKLTKVFKDIRDYVSNKLEKTTIADIQ"
     gene            3269..>4113
                     /locus_tag="PLR93_05885"
     CDS             3269..>4113
                     /locus_tag="PLR93_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH26529.1"
                     /translation="MRKQTLTWLSLFLVLAVSLTACGTAAPEATVAPASDATDVAATE
                     TAPTEEPLSGTLSISGAFALYPMMTVWAEEFTKLHPDVQFDVQGGGAGKGMTDTIAGA
                     VDIGMISRSIKPEEEAQNIFWVSVTKDAVFPIINAENPVAQQILEKGISQEIFAKIYI
                     TGEITTWGEVVGDPSITDKINVFTRSDASGAAEQWAKYAGGKAQEDLKGIGVNGEPAM
                     LDTILKDPLGIGFGNLNSIFDLSGGGLVPGIVIPPIDINENGAADTEEIYTVKEDAFS
                     AVANGT"
BASE COUNT         1176 a         1000 c          854 g         1083 t
ORIGIN      
        1 tggagttccc tccatctctc aaattgtaac attgcacgtt gtttatcttt gcactaacac
       61 aaatgtgcct gtatagttta caaacgctgc aaaataggtc ttgccatttt cggtgagtac
      121 aaaggtgcca ttcacttctg tccattgaga accatcccaa tacagaatgg cgagggcaga
      181 agcatctacc ccttctggca taatgaatga aacaagaacg ggctggctta acttgccgtc
      241 tacagacccg ctctcagtaa caactgagac aatgcccgag cggaatgttg aaccacttgg
      301 caacgaggat ggtaaaccat ttgcatcttg cttggtcagg ctggcagaac cacccagcgg
      361 gcaaggcacc acaaggttat cgccattttc caatactagt ttggtgccac tatagttttg
      421 gcaatctaga tcgattccat taccactgcc tacgttaacg attcttaacg ggaggttggt
      481 atttccttta ccgcctccgc taaacgcgtt tacagggcaa ttcgggtcag aggtccatgt
      541 accgccgcta gaaggaggaa caaagtctgt tgtatttccg ctaaagctaa cacctctcat
      601 cgtgagaaca ctgcccgtag gaatatctac agcaagacca actgcttcat ttgcaaagaa
      661 gctgcaatac acatcaacat tcccacccac tgtgatattc gcaccaccgc ctaacatagt
      721 cccattatta ctggcggtta caaaattgaa atagacattg ccaggggttg caacctgaat
      781 cccagcccca atagagcaag tacctgtgtt gcagtttccg ttaaaaacac tattctctac
      841 aaaaatatta ccatttgaaa tgattaagac accatttccc gtatcagaat tgtatgtaga
      901 accatctccg ttgccattcc cgtcggcagt aacatttttt attgtaacag tgcctacatc
      961 attgagtgta aataaaccaa aacccatatc acactgatag gttgtacagt tatcatcgaa
     1021 tgtgctgttg gaaatggata tattcccatt cccagctaat acaaatgcac caccgcctat
     1081 atccccagcc ccattccctg taaccgtggt gccactaatg gagatattat cagcagctgt
     1141 aacccatagc ccaacacttg caggtccgat aaaccacgaa cctggagtac cgccattatt
     1201 cttaattgta tttgtgcctg tcattgtgat cgagacaagg ttggtattaa aacacggact
     1261 accgccaaag attccgcagt tatccaattc cacgccgcca acttcattca attcggcatc
     1321 gatactatct actgtaatat caccaattgc atacacaacc aatccacgat cattattctc
     1381 aataaaagtg cttgattgaa cgaagacatt acccgttccg cagccacatg aagtatcaat
     1441 ataagcgcct gaaagatcat tattgtcaaa aacactgttc ttaatagtta tgtcgccatc
     1501 tgcttcaatg tatgcaccat cgccatcatt ctgtcttgct aacacttgct ctaaggtaac
     1561 atcgcctcca tacgagtaaa tagttagccc attatcattt gccgtattat taaatttact
     1621 attggtaatt tctatatccc catctgtttc gatataggtt ccaaattcag ttgaatctac
     1681 ttcaacattc tccaatgtaa tatcggcagt tgtaacatca ctgtcatcac caataaataa
     1741 gccatcgcta tttgtaatga aaagattctt aagggtaagt gttgccggag ttccgtagcc
     1801 accccaatcc caaaactcta gtgaataccc tggttttagg tcaatggtag aagttcccac
     1861 gactttgtct tgggtaaaat cccaaccgcc ttgaacaacc aaatcagtaa gatccaatgc
     1921 cccataatca aaaataatgt cgttgctcga atcaccagct gtatttgcag catagtcata
     1981 agcaacgtag atcgtacctg agccataata tgtagcacta tttgtttgca agtcattgat
     2041 caggtcattg aagctggtat gcgcaactgt acattgaccc agagtatcgg ttccaggtgt
     2101 gacgcctgct gggcaccatt gcgggtcacc ggtcaccaaa acattggcag cctcgttcga
     2161 aaccaatggc aaaggttcgc tattttcatc aagaacaacg atctcagtct cagccggtaa
     2221 ttgttctaaa atttctggaa cagtttgctc caacgtttct tgagtctcag aagattcctc
     2281 gactggaggc gtttcggttg gaacttcgtc tgtgggggtg ggaggcgcat catctgccaa
     2341 gacacttcga gtgccgatca tggacacagt cagggctaga ataagtgcaa tggggaaaag
     2401 gaatttcagg tacttgttct tcatacaata aattctccat gctaaggttt ctgaacaaag
     2461 taagccttac aatataaatt tggactattc tactcttaat gtaatcttgt acattagatt
     2521 tggtcgccaa aactgttaag aaagggctgt ttggaaacaa aaaccgcctg aggtttctca
     2581 ggcggttcat ttatattatt gaatgtctgc aatcgtggtc ttttccaatt tattcgaaac
     2641 ataatctcga atatccttga ataccttggt cagcttcttt tctttctcaa ttggcgtgga
     2701 ctcataaaaa ttctcactga ccgactcggt gggggcaagg gcaccatcaa aaagacgaat
     2761 aacttccgcg agggaaatct gatcgggacg tttggcaagc tgatagccgc ctttttgtcc
     2821 cttggcgctg cgcaagaaat gcgcacgctt gagtgccagc atgagctgct ctaagaactt
     2881 aggcggaatc ccttgggctg tggctatggt atcaatggaa ataaaccctt catcttcatt
     2941 acgggcaaga taaacaaggg caaggagggc atattcacta cgagtggtta gcttcatcta
     3001 ataactcaac ttttctgatt tgacctacgg gagcagtata gtataaaaaa taagcatgtc
     3061 aagtcgaggc aaaatggtcg gttcaaacat ctttcagtat tgacaaaatt cagtcgctga
     3121 attatgataa gtacacataa cctattagtc tagtaggatt tatcaagata ctcgaggtct
     3181 tgaaataaaa gaagtctttt tatttaactt aaattcctac taaatcaata ggctttatat
     3241 aaaaaacaaa cgtaattgga gaaacaatat gcgaaaacaa actttaacat ggctgtcgct
     3301 attcttggtt ttggcagtat ccctcacagc gtgcggaact gccgcgccgg aagccacagt
     3361 cgcccctgcg tctgatgcga ctgatgtcgc tgccacagaa accgccccca ccgaagaacc
     3421 tctcagcggc acactgtcca tctctggcgc attcgccctc taccccatga tgactgtttg
     3481 ggcagaagag ttcaccaagt tacaccctga cgttcagttc gatgtgcagg gcggcggcgc
     3541 gggcaaaggc atgaccgaca ccattgcagg tgccgtggac attggcatga tctcgcgcag
     3601 tatcaaaccg gaagaagaag ctcagaacat cttctgggtg tccgtgacga aggatgcggt
     3661 cttcccgatc atcaatgcag aaaatcctgt tgctcaacag attcttgaaa aaggtatttc
     3721 gcaagaaatc tttgccaaga tttacatcac cggcgaaatt accacctggg gcgaagtggt
     3781 cggcgaccca tccatcacgg ataagatcaa tgtcttcact cgctccgatg cctccggtgc
     3841 tgccgaacaa tgggcgaagt acgcaggtgg aaaagcccaa gaagacctga agggcatcgg
     3901 cgtgaacggc gaacccgcca tgctcgatac cattttgaaa gacccgctcg gcattggctt
     3961 cggcaacctc aacagcattt tcgatctgag cggcggcgga cttgtgcccg gaatcgtcat
     4021 cccgcccatc gacattaacg agaatggcgc agcagacacg gaagaaatct acaccgtgaa
     4081 agaagatgcc ttcagcgcag tggcgaatgg aac
//
LOCUS       DAOQFT010000182         4102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_11875_length_4102_cov_6.291821, whole genome shotgun sequence.
ACCESSION   DAOQFT010000182 DAOQFT010000000
VERSION     DAOQFT010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11875_length_4102_cov_6.291821"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLR93_05890"
     CDS             complement(<1..598)
                     /locus_tag="PLR93_05890"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNH26530.1"
                     /translation="MQERLQKILAQAGYGSRRACEEFIIEGRVSVNGQIAILGQKADL
                     AVDTIKVDGKSLSKPETMTYIALYKPRNVLSAAEGQDDRKTVRDLIPAPGHLYPVGRL
                     DFDSEGLILMTNDGDLTNKLTHPRYAHEKEYRVLLACKPDTEQLDAWRRGVVLPEGDR
                     TQPANVHFISTSGKGAWIRVVMGEGKKRQIREVGKLLGL"
     gene            complement(601..1170)
                     /gene="scpB"
                     /locus_tag="PLR93_05895"
     CDS             complement(601..1170)
                     /gene="scpB"
                     /locus_tag="PLR93_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNH26531.1"
                     /translation="MSESEPITNEELSLAAKIEAMLFVSADPVPVQQLSQALDVTASV
                     VERGLKELDEVLKARGLRLQRNAGRVQLTTAPEIAGLVETFLGLEAITHLSRAALETL
                     AIIAYQQPVTRPQIDSIRGVNSDGMMKSLLSKGLIQESGRTEGPGRPILYSTTPEFLQ
                     HFGLSSIMELPPLATPIETEPEPQELLKG"
     gene            1215..1784
                     /locus_tag="PLR93_05900"
     CDS             1215..1784
                     /locus_tag="PLR93_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26532.1"
                     /translation="MKTKNYIYLFIVSLVSASLACSINVGGPEYPAQPISASPTDAQA
                     LRDMIQQAMVTGAETGVITLSVTESQLTGYMAEKLSQQTNPPFTDPQILLRNGQMQMY
                     GKVTQGWFTANMLITMNVTIDETTGQPKIQITSADFGPIPAPEGVNNAISAIIEEAFT
                     GSFGPVAVGFRLQTITIADGIMTLTGRIK"
     gene            1804..3393
                     /locus_tag="PLR93_05905"
     CDS             1804..3393
                     /locus_tag="PLR93_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNH26533.1"
                     /translation="MPRLTLRPSLLATILLLVTLLASCKSPQLNSDITITVTADGTTR
                     DITVPQGQTVSQALESAGITVNSLDKTEPPLYTVIGNGDLVTLIRVEEVFETEEIVVP
                     FERQVVRNETLPEGETRLVQAGVNGLEEITYRKILEDKVETSQSIVKTTVIKEALPEI
                     VMVGAQTSFVPLNIPGTLAYLAGGNAWIMEGSTANRRVIVSTGDLDGRIFTLSPNGEY
                     LVFTRKSSKPADEEINTLWAVRTRNLDPKLISLQAKNVVHFAAWIPGTNSIAYSTVEP
                     RSAAPGWQANNDLYRVSITNSGSPKRILYPSSGGVYGWWGMNFAFAPDGRLAYSRPDG
                     IGLVDQDSGRLKPLLDITPLNTHSDWAWIPPLVWGADGNGLYFVSHAPAPAPISGEES
                     PYFDLSAISFKNEAAVSLVNSAGMFSYPAISPIQYGSREAAFQIAYLQSIFIDQSDSS
                     RYRLVVMDRDGSNSRALFPPADKNGLEPQTPVWAPAAVEGQSGEFIAVMYQGNLWLID
                     SGNSNTYQVTGDGLITRLDWK"
     gene            3417..>4102
                     /locus_tag="PLR93_05910"
     CDS             3417..>4102
                     /locus_tag="PLR93_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH26534.1"
                     /translation="MRILITGAAGFLGSHLCDRLLADGHEIIGMDNFITGNPDNIAHL
                     AGNEKFQFFKRDVSNYIFVPGKVDAILHFASPASPNPQSKSGYFNLPIQTMKAGALGT
                     HNCLGIARREGSRFLLASTSEIYGDPEIHPQAETYPGNVDPVGERAVYDEAKRFAESM
                     TMAYHRFHNVNTSIVRIFNTYGPRMDLEDGRALPNLLKQALLGEPLTVYGTGSQTRSF
                     CFVDDLVDGIV"
BASE COUNT         1054 a         1089 c          927 g         1032 t
ORIGIN      
        1 gcaaaccaag caatttaccc acttcccgaa tctgacgttt cttcccttcc cccatcacca
       61 cacgaatcca tgcacctttg ccagatgtgg aaataaagtg aacattcgca ggctgggtac
      121 ggtcaccttc tggaagaaca acgcctcggc gccaagcatc aagctgttca gtatccggtt
      181 tacaagcgag cagcacgcga tattcttttt catgtgcgta tcgcggatgt gtcaacttgt
      241 tcgtgagatc cccatcattg gtcatgagaa tcaagccttc ggagtcaaaa tcgaggcgtc
      301 ctactggata taaatgtcca ggagcgggaa tcaaatctcg aaccgtttta cgatcgtctt
      361 gcccctctgc agcagagagg acattacgag gcttgtataa ggcgatatac gtcatcgttt
      421 caggctttga aagtgactta ccgtccacct taatggtatc cactgccaga tctgccttct
      481 gccccaagat tgcaatctgc ccattgaccg aaactcgccc ctcaataata aactcctcgc
      541 aagcacggcg ggaaccatac ccagcctgtg cgagaatctt ttgcaaacgt tcctgcataa
      601 ttaacctttc aacaattctt gcggttcagg ttcggtttca attggagttg ccaatggcgg
      661 caattccata atggagctca aaccaaagtg ctgtaaaaat tccggcgtgg ttgaataaag
      721 aataggacga cccggacctt ctgtgcgtcc agattcctga atcaaacctt tgctcaacaa
      781 gcttttcatc ataccgtcgc tattcacacc acgaatcgaa tcgatctgtg gacgcgtcac
      841 cggctgttgg taagcaatga tcgccaatgt ttctaacgcc gctcgactga ggtgcgtaat
      901 agcctccaac ccaaggaagg tttccaccaa tccagcaatc tcaggggcgg tggtcaactg
      961 cacacgccct gcattgcgct gaagccgcaa gccacgagct tttaatactt cgtctaattc
     1021 tttcagccca cgttccacaa cagacgcagt cacatccaac gcctgcgaca gttgttgcac
     1081 tggtaccggg tccgctgaaa cgaataacat cgcctcgatc tttgccgcca ggctcaattc
     1141 ttcatttgta atgggttcag attcgctcat gctataattg ctccgcagtc catatttcaa
     1201 acgttcacaa aatcatgaaa actaaaaact atatttacct ttttattgtt tcgcttgtca
     1261 gtgcatcact agcatgctct atcaatgtcg gcggaccaga atatccggct cagcccattt
     1321 cagcctcacc aaccgacgcc caagcactac gcgatatgat ccaacaagcc atggtcactg
     1381 gtgccgaaac cggcgtgatc accctttccg tcaccgaaag tcagctaacc ggctatatgg
     1441 cagaaaaact ttctcaacaa acaaacccac cgtttactga tccacaaatc ttgcttcgca
     1501 acgggcaaat gcaaatgtat ggcaaggtta cacagggttg gtttacagcc aatatgctca
     1561 ttaccatgaa cgtaacgatc gatgagacca caggtcaacc caaaatacag atcacatctg
     1621 cagactttgg tcccatccct gcgccagaag gtgtcaacaa cgccattagt gccattattg
     1681 aagaagcctt caccggttca ttcggtcccg tagctgttgg ctttcgatta caaaccatca
     1741 ccatcgcaga cggcatcatg accctgaccg ggcgcatcaa atagcggctg gattataccc
     1801 gacatgcccc gcctcaccct ccgcccgagc ttgctcgcca ccatcttgct tttggttaca
     1861 ctgttggctt cttgtaagtc gccgcagctc aatagtgaca ttacaatcac agtcactgca
     1921 gatggcacta ctagagacat cactgttccg caagggcaaa ccgtctcaca ggcgttggaa
     1981 agtgcaggga tcaccgtaaa cagcctcgat aagacagagc cgccgctcta taccgtcatt
     2041 ggcaatggcg acctcgtcac cttaattcgg gtggaagaag ttttcgaaac cgaagagatc
     2101 gtcgtcccgt ttgaacgaca agtcgtccgc aacgaaacct tgcccgaagg cgaaacccgt
     2161 ctcgttcagg cgggcgtgaa cggacttgaa gaaatcacat atcgcaagat attagaagat
     2221 aaagtagaaa ccagtcagtc cattgtaaaa actacggtga tcaaagaagc tttacctgag
     2281 attgtcatgg tcggcgcaca aacttctttt gtgccgctaa acattcccgg cacgttagcc
     2341 tatctagctg gcggcaatgc ttggatcatg gaaggctcca ccgccaatcg ccgtgttatc
     2401 gtcagcaccg gagatttaga tgggcgcatc ttcactctct cgcctaacgg tgaatatttg
     2461 gtttttacgc gcaaatccag caaaccagca gacgaagaga tcaataccct ttgggcagtt
     2521 cgcacccgca accttgaccc taaattgatc tcattgcaag caaaaaatgt agtccacttt
     2581 gcagcatgga ttcctggcac gaattcgatc gcgtattcca ccgttgagcc gcgcagtgcg
     2641 gcaccaggtt ggcaagcaaa caatgacctg taccgcgtca gcattacaaa cagtggttca
     2701 cccaaacgca tcttatatcc aagcagtggt ggtgtgtatg gttggtgggg catgaatttt
     2761 gccttcgccc cagatggtag actcgcttat tctcgtcctg atggaattgg cttagtggac
     2821 caagatagcg gtcgcttaaa accattacta gatattactc cgctgaacac acatagtgac
     2881 tgggcgtgga ttccgcctct cgtttggggc gcagacggaa acggtttata cttcgtcagc
     2941 catgctccgg ctccggcacc catttccggc gaggagtcgc cttattttga cctctcagcc
     3001 atctcgttta agaatgaggc ggcagtgtct ttggtcaatt cagcaggcat gttctcatat
     3061 cctgccattt ctccgatcca atatggaagt agagaagcgg catttcagat tgcctatcta
     3121 cagtccatct tcatcgacca gagcgactca agccgctatc gtttggttgt aatggaccgg
     3181 gatggttcaa acagtcgggc actctttccg cctgcggata agaatgggtt ggaacctcaa
     3241 acacccgtgt gggctcccgc tgcggtggaa gggcaaagtg gcgaatttat tgctgtgatg
     3301 taccaaggaa atctttggct tatcgatagt ggcaatagca atacctatca agtcaccggt
     3361 gatggattga tcacccggtt ggactggaag taaataaaaa gaagaggtgt aacataatgc
     3421 gaattttgat cacaggtgcg gcagggtttc ttggctctca tctctgcgac cgcttactag
     3481 cagatggtca cgagatcatt ggcatggata attttattac gggcaaccca gataatattg
     3541 cccacttggc ggggaatgaa aagtttcaat tttttaaacg tgatgtatca aactatattt
     3601 tcgttcctgg caaagtagat gccattctgc attttgcctc tcctgccagc cctaacccac
     3661 aatcaaaatc tggttatttc aatttaccca ttcaaaccat gaaggctggc gctttaggca
     3721 cccataactg cttgggcatt gcaaggcgtg aaggctcgcg cttcttgctt gcctctacca
     3781 gtgaaattta tggtgatcct gaaattcacc cgcaggcaga aacatatcct ggtaatgtag
     3841 acccggtcgg tgagcgcgcc gtctatgatg aagccaagcg ttttgcagaa tccatgacta
     3901 tggcgtatca ccgtttccat aatgtgaaca ccagcattgt gcgcatcttt aatacgtacg
     3961 gtccacgcat ggatttggaa gatgggcgtg ccttgcctaa cttactgaaa caggcattgt
     4021 taggagagcc tcttaccgtg tatggaactg gcagccaaac ccgttctttc tgcttcgttg
     4081 acgatttggt ggacggcatc gt
//
LOCUS       DAOQFT010000183         4070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12031_length_4070_cov_6.879203, whole genome shotgun sequence.
ACCESSION   DAOQFT010000183 DAOQFT010000000
VERSION     DAOQFT010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12031_length_4070_cov_6.879203"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLR93_05915"
     CDS             complement(<1..180)
                     /locus_tag="PLR93_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNH26535.1"
                     /translation="MKVSFQGEPGAYSEQAVFNYFGKDVETIPCESFDVMFDSVVSEK
                     SDMALAPIENSLAGSI"
     gene            complement(230..1411)
                     /locus_tag="PLR93_05920"
     CDS             complement(230..1411)
                     /locus_tag="PLR93_05920"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH26536.1"
                     /translation="MQTLNHQFELIRQSATVSLTDRILALKASGKTIISLQVGDPDFG
                     THPAIAEVALKAMQEGQTHYGPSNGTLELRKAIAHKLQRDEGISYDPNTEILVTNGGI
                     HAYYTAMQSILNPGDDVLIPDPSWATHSNMATMLRGNVIRIPAPAENGFIPHFDSWLK
                     ALTPKTRVIVLNYPSNPTGAYPTREYLQQLQNFAAKNNLWIVSDEVYGSLYYGDKPTS
                     AAAIEGAKDRTLLVHSLSKSYAMTGWRIGFLAAPANVISNALKASQNSITCVAPFIQK
                     AAAFALTDPNMQEVTQTMRAGYAKRRELVLKLARDYESDKVIVTPSQGAFYFFLDMRP
                     LKMSSAEICEKLLDDVGVALVPGSAFGEQGEGFARLCIAASDEDVEAGFRKIVEWAER
                     Q"
     gene            complement(1481..2746)
                     /locus_tag="PLR93_05925"
     CDS             complement(1481..2746)
                     /locus_tag="PLR93_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH26537.1"
                     /translation="MTDSQTKILSRLLEVTRSLDSEADLESSLRLILSAATELTASET
                     ASLLEYDETALNFFFKYVPWFNQDSQGGSTLRIPLNESIAGWVLLNKSSVTVNDAIND
                     PRHSKKVDEFAGITIHSVLGAPLIVRDVPIGVLEVFNKRSNYTEEDVLIVENLAALAA
                     AAMEKDLLEKKVASSKEEARELDRMKNEFIAITSHELRTPLGLILGHSTFLKELLGGS
                     YEDQVDVIIRNASRLKEIIESLTSVDNYQSGGALVRSRKVSIARIIEDVIVSFSEMAN
                     KKKITIKKALQPGYELWVDVDAGKVAIVLSNIMKNAITFTNEGGEIIIRGEQHPDYVK
                     VSVKDNGIGIPTKDLPHVFDRFYQVEGHLTRRHGGMGLGLSVAKVMVEMHGGRIWADS
                     EEGKGSIFSFILPVRSEPPAPASSSPFSI"
     gene            2846..3934
                     /locus_tag="PLR93_05930"
     CDS             2846..3934
                     /locus_tag="PLR93_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNH26538.1"
                     /translation="MANKKKVRVAIIGVGNCASSLVQGVQFYKNAKNDDVIPGIMHPQ
                     LGEYHIGDIEFTLAIDINATKVGKDLSEAIFAEPNNTYKFCDVPHLNVPVVRGMTHDG
                     LGKYLSQVIEKAPGSTADIVRLLKETKTEVVISFLPVGSEMATKWYVEQIIEAGCAFV
                     NGIPVFIASSEYWGKRFADAGLPILGDDIKSQVGATILHRQLATLFVDRGVKIERTYQ
                     LNFGGNTDFLNMLERERLESKKISKTNAVTSMIPYEINPDNVHVGPSDHVPWLTDRKW
                     AYIRMEGTTFGNVPLNMEVKLEVWDSPNSAGVMIDALRCAKIALDRELAGPIVGPSSY
                     FFKTPPKQFRDEIAKQKVEDFVSGKSDE"
BASE COUNT         1093 a         1012 c          938 g         1027 t
ORIGIN      
        1 aatactgccc gccagtgaat tctcaatggg agccagtgcc atgtcgcttt tctctgaaac
       61 cactgaatca aacatcacat cgaacgattc gcatggaatg gtttcaacgt ctttaccaaa
      121 ataattaaag accgcctgct ctgaatacgc accaggttcg ccttggaaag atactttcat
      181 aagaaactcc attgggcggg aagactccgc ctcataactg ataactgggt tattgtcttt
      241 cagcccactc tacgatcttc ctgaaccccg cttccacatc ttcatctgaa gcggcaatgc
      301 ataaacgagc aaagccttcg ccttgttcgc caaatgccga gccgggcaca agcgccacac
      361 caacatcatc caatagcttt tcgcagatct ccgctgaaga catcttgagc ggacgcatat
      421 caaggaagaa ataaaaagca ccctgcgaag gtgtgacgat gactttgtcg ctttcatagt
      481 cgcgagcaag ttttagaacc agctcacggc gtttggcata gcctgctcgc attgtctggg
      541 tcacttcttg catgtttggg tctgtgagcg caaaggcggc ggctttttga atgaagggag
      601 ccacacaagt gatggagttc tggctggctt tcaaagcatt ggagatcacg tttgcaggcg
      661 cagcaaggaa gcctatgcgc caacccgtca tggcatagga cttggacaag ctgtggacca
      721 ataacgtccg atctttcgcg ccttcgatgg cagcagcact ggtcggttta tccccgtaat
      781 acaaactgcc atagacctca tcactaacaa tccataaatt attttttgca gcaaagtttt
      841 gaagctgttg caagtattcg cgagtgggat atgcaccggt cgggtttgag ggatagttca
      901 gaacaatgac tcgggttttg ggagtaagag ctttcagcca agagtcgaaa tgagggatga
      961 acccattctc ggcgggggca gggatgcgaa tgacatttcc acgcagcatg gttgccatgt
     1021 tggaatgagt tgcccaggaa ggatccggta tcaagacgtc atcacccgga ttcaaaatgg
     1081 attgcatcgc cgtatagtat gcgtgaatgc cgccgttggt gacgagaatt tctgtattag
     1141 gatcgtagct tatgccttca tcgcgttgaa gtttgtgcgc gatcgctttg cgaagttcaa
     1201 gcgtgccgtt ggaaggtcca taatgagttt gaccttcttg catggcttta agcgccactt
     1261 cagcaatggc tggatgcgtg ccaaaatcgg gatcaccgac ttgcaagctg atgatggttt
     1321 tgccgctggc tttgagcgcg agaatgcggt cggtcaatga gactgtggca gattggcgga
     1381 tcaattcaaa ttgatgatta agagtttgca tagaatgtcc ttcaaagaat gggcgtgagt
     1441 gtatagggaa ataaaaatcc cgtcaacggt gtgacgggac tcaaatggaa aagggagaag
     1501 aggatgctgg cgctggcggc tcgcttcgaa cgggaagaat aaaggaaaag atactccctt
     1561 tgccttcttc gctatcagcc caaatacgcc cgccatgcat ttcaaccatc accttggcaa
     1621 cggaaagtcc caaccccatg ccaccgtgtc ggcgtgtgag gtgaccttcc acctgataga
     1681 agcggtcaaa cacgtgtggc aagtctttgg taggaatccc aattccgtta tctttcaccg
     1741 agacttttac ataatccgga tgctgttcac cacggatgat gatttctccg ccttcattcg
     1801 taaatgtaat agcgttcttc ataatattgc tcagaacaat ggcaactttc ccagcatcta
     1861 catccaccca caattcatac ccaggctgaa gagctttttt gatcgtgatc tttttcttat
     1921 tcgccatttc agaaaaagag acgatcacat cttcaataat tcttgcaata gaaactttgc
     1981 gcgagcggac caatgcccca ccgctctggt agttatccac gctggttaag ctctcgataa
     2041 tttctttcaa acgagaggca ttacgaatga tgacatccac ctggtcttca taactcccac
     2101 ctaataattc tttcaagaac gtagaatgtc caaggatgag acccaagggc gtgcgcagtt
     2161 catgtgatgt gatcgcgata aactcattct tcatccgatc aagttcacgc gcctcttctt
     2221 tcgacgaagc gactttcttc tcaagaaggt ccttttccat cgctgcagcc gccagtgccg
     2281 ccaagttttc aacgattaga acatcttctt ctgtgtagtt actgcgttta ttgaagactt
     2341 ccaaaacccc aatcggcaca tcgcgaacaa tcaacggtgc ccctaaaacg gagtggatgg
     2401 ttatgcctgc aaattcatcc acctttttgg aatgacgcgg gtcattgatc gcatcgttca
     2461 cagtcaccga tgatttattc agcaaaaccc aaccggcaat actctcattc aacggaatac
     2521 ggagcgtact tccaccttgt gaatcttgat tgaaccaagg gacatatttg aaaaagaaat
     2581 ttagagccgt ttcatcatat tccaaaagag acgcggtttc gctcgccgtc agttcggtag
     2641 cggcagagag gattaaacgt aaactggact ccaaatccgc ttcagagtcg aggctgcgtg
     2701 tcacttcgag gagacgtgaa aggattttcg tctgtgagtc cgtcatacaa ataaccttgg
     2761 gtaagattat acttcacggt tgtatctgaa aatcgaccct cataattcaa taaggaaact
     2821 gtaatccacc tcaggagcta aacaaatggc aaataagaaa aaagtacgtg tggcgatcat
     2881 tggcgtagga aactgtgcct cttccctcgt gcaaggcgtt caattctata aaaatgccaa
     2941 aaatgacgat gtgattcccg gcattatgca cccgcaactg ggtgaatatc acattggtga
     3001 cattgaattc accctggcga tcgacatcaa tgccaccaag gttggcaaag atttgtctga
     3061 agcaattttt gcggagccga acaataccta caagttctgt gatgtgcctc acctcaatgt
     3121 acccgtcgtg cgcggcatga cccatgatgg gttgggcaag tatctcagcc aggtcattga
     3181 gaaagcgccc ggctccactg ccgatattgt acgcctatta aaagaaacaa agaccgaagt
     3241 tgtcatcagc ttcctcccgg taggctcaga aatggcaacc aaatggtatg ttgagcagat
     3301 tattgaagca ggttgcgcct ttgttaacgg tattccggta ttcattgcct cctctgaata
     3361 ttggggcaaa cgcttcgccg atgccggttt gccaattctc ggtgacgata tcaaaagtca
     3421 ggtcggtgcc accattcttc accgccagct cgccaccctg ttcgtagacc gaggcgtcaa
     3481 gatcgagcgc acctatcagc tcaatttcgg cggcaataca gacttcctca acatgctcga
     3541 gcgcgaacgc cttgaatcca agaaaatttc caagaccaac gccgtcacca gcatgatccc
     3601 atacgaaata aatccagata atgttcacgt cggtcccagc gatcacgtcc cctggttaac
     3661 ggatcgcaaa tgggcataca ttcgcatgga aggtaccacc ttcggcaacg ttccgctcaa
     3721 catggaagtg aaacttgaag tgtgggatag ccccaactct gccggcgtta tgatcgatgc
     3781 tctacgctgt gccaagatcg cgctcgaccg cgaacttgcc ggtccgattg ttggaccatc
     3841 ctcttacttc ttcaagactc cgccaaagca attccgcgat gaaatcgcaa aacaaaaagt
     3901 ggaagatttc gtctctggca aaagtgatga ataacaaata acagaaaagg cgcacagaaa
     3961 tctggtgcgc cttttctgtt attattgaca atcacaaaag gatataaatt catcatgcga
     4021 cgcaaaacca ctcgtcactc gttaatcgtt tctcgcctct ttcttagcct
//
LOCUS       DAOQFT010000184         4067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12052_length_4067_cov_5.349701, whole genome shotgun sequence.
ACCESSION   DAOQFT010000184 DAOQFT010000000
VERSION     DAOQFT010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12052_length_4067_cov_5.349701"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLR93_05935"
     CDS             complement(<1..875)
                     /locus_tag="PLR93_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNH26539.1"
                     /translation="MAKIPARKQVNKKYTWNAESVFKTAKDWEAALKAVVEDIPNVKK
                     FEGKLGESPETLYEGMQAIEKISLRAQTIFMYAQFAYAVDTTNQESAAMVGKAQGMAG
                     QVFAAISFLNPELLYIGKEKLDAWMNSFEKLSTYRQSFDDLFRQQAHVRSAEVEEILG
                     MAADPFSGPSSSTSMLVNADFKFAPAQDSKGKKVEITQSNFYSTLLEHPDRKVRQTAF
                     NSYHDKHVEFKNTLATNLSSSISANVFNMRARKFETTLSASLFNNNIPEEVFHNLINT
                     FKSKLPVWHRYFDIKR"
     gene            complement(954..2528)
                     /locus_tag="PLR93_05940"
     CDS             complement(954..2528)
                     /locus_tag="PLR93_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH26540.1"
                     /translation="MSIVPSMLPDFRVRQRDYLLEIARLLTQELDLEKLMARILRVSV
                     EMLAGQAGIIALKLPDGWRVAAAHGIAPAFLSYLTPLLAEEKVRELDVTELNRMLKEL
                     TYTASMGLLNGTGLPLAAHGQVIGVIFIFRNYPDVFTPNDRILLQSFADQAAIAVFNA
                     QLYGQVTYEKKRLDALLDSAGDGILILDAELNIERCNEAFSKLYGSTREEIKDKPHKE
                     IIKWKKEPHGSTLEESIVNGWPLTPNATLYVEGDLSRPLPPTLPVGITYAPLLSDEGK
                     LRNVIVAVRDITHFRNADEMKATFISIVSHELRTPVALIKGYASTLRRADAKWDKNTI
                     NDSLTVIEEEADRLSKMIDDLLDASRLQAGGMSLNQADVALPSLALRVSERLASQSPK
                     HLIVTDFPEKFPIILGDENRLEQVISNLVSNALKYAPEGEIKIIGKVLPEQVVVCVSD
                     EGPGIDPKDMPHIFDRFYRSTSAVKQTKGAGLGLYLARAIIEAHGGRIWAEGANSSTQ
                     TSKPESGARICFSLPR"
     gene            2856..>4067
                     /locus_tag="PLR93_05945"
     CDS             2856..>4067
                     /locus_tag="PLR93_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26541.1"
                     /translation="MIRSVRSILMAFFLAVSVLVVQVSPVQALTYPAEINMSFTPISI
                     TSGGTSRLRVTIYNPNSNALTGAAWLDNLIGVQPGLSIAATPNVVQTCTGTVTDSGGG
                     AVDPGDTSIRLTGGTVPGQVGSNPGSCYVEVSVTSTTPGNLINTIPASALTSFTTDPQ
                     DTNTPPAQVPITNTTPASATLNVVAVQPPSVSKGFAPNTIFVGQVSQLTITIRNNDLN
                     AALTNTTFTDTLPVGVVVATPLTTNLTGCGAGTLTSTLGGPALAAGDTAISLNGGTIA
                     RNSNCVITVNVTSVLENSYTNTIPAGPPGSGSVQTQQGVTNAGPASAPLNVQAFNLSK
                     SFSTSPIAVGGVSQLSIVIQNPANFNYTGVEITDNLPAGLAIAIPVNTGSTGCGSPTF
                     TDAGGGALGAGD"
BASE COUNT          956 a          960 c         1011 g         1140 t
ORIGIN      
        1 cgtttgatat cgaagtaacg atgccaaaca ggcagcttgc tcttgaaggt gttgatgagg
       61 ttatggaaga cttcttcagg aatgttgttg ttgaaaagag acgccgagag agtcgtttca
      121 aatttgcggg cacgcatatt gaaaacgttg gcgctaatgg atgaagaaag gttggtggct
      181 aaagtgttct tgaactccac atgcttatcg tgatagctgt tgaatgcggt ctgacgaact
      241 ttgcggtctg ggtgttcaag taaggtcgag tagaaattgc tttgggtgat ttccaccttc
      301 ttgcctttgc tatcttgtgc tggggcaaat ttgaaatcgg cattgaccaa catgcttgtg
      361 ctgctggaag gtccgctgaa tggatcggct gccataccaa gaatctcttc cacctccgcc
      421 gagcggacgt gtgcctgttg gcggaagagg tcgtcaaaac tctggcggta ggtgctcagc
      481 ttttcgaatg aattcatcca cgcatcgagc ttttctttac caatataaag caattcaggg
      541 ttcaagaaag aaatagcggc aaaaacctgc cctgccatgc cttgtgcttt gccaaccatg
      601 gcagctgatt cttgattggt cgtatccacc gcatacgcaa actgagcgta cataaaaatg
      661 gtttgcgccc gcagagatat tttttcgatg gcttgcattc cttcgtaaag agtctctggg
      721 ctttcaccta gcttcccttc gaacttcttg acattgggaa tgtcttcaac cactgccttc
      781 aaggctgctt cccagtcttt ggcagttttg aaaacacttt cagcgttcca ggtatatttc
      841 ttgttcactt gctttcgcgc aggaattttc gccatgagtt tgtccttttt aaataggttg
      901 ttatgatttt accttttccc gcatgtaaaa cgggagaccc tagagtaggg gaattaacgt
      961 ggtaacgaga agcaaatccg ggcacccgac tccggtttcg atgtttgagt cgagctgtta
     1021 gcaccttcag cccaaatccg cccaccatgg gcttctatga tcgcacgagc aaggtacaag
     1081 ccgagcccgg ctcctttggt ttgtttcacc gcactggtcg aacgatagaa ccggtcaaag
     1141 atatgcggca tatctttcgg gtcaatgccg ggtccttcat cactaacgca cacaaccacc
     1201 tgctcgggca aaaccttgcc aatgattttg atctctcctt caggtgcata cttcagtgca
     1261 ttcgagacga ggtttgaaat gacttgttcc aatcggtttt catcgccgag gatgatagga
     1321 aacttttcag ggaagtcggt gacaatgagg tgttttggtg attgggatgc caaccgttcg
     1381 ctcactctta acgcaaggct gggtagggca acatcggctt gattcaagct catgcctcca
     1441 gcttgcaagc gcgaggcgtc tagcagatca tcgatcatct tcgagaggcg gtctgcttct
     1501 tcttcaatga cggtgagtga gtcgttgatg gtgttcttat cccattttgc atctgcccgc
     1561 cgcaatgtag aagcatatcc tttaataagg gctaccggcg tccgcagttc atggctgacg
     1621 atagaaatga aggttgcctt catctcgtct gcgtttcgga aatgggtaat atctcggact
     1681 gccacaatca cattacgtag cttgccttca tctgaaagca gtggagcata ggtaattcca
     1741 actggcaagg ttggcggcaa aggacggctg aggtcgcctt ctacatagag tgtggcattg
     1801 ggtgttaacg gccagccatt tacgatagac tcctcaagcg tgctgccgtg aggttctttc
     1861 ttccacttga taatttcttt gtgaggcttg tctttgatct cttcgcgagt tgaaccatac
     1921 aatttgctga aggcttcatt acaacgttca atgttcaatt cggcatcaag aatgagaatg
     1981 ccatccccag ccgagtcgag cagggcatct aggcgtttct tttcgtaggt aacctgtccg
     2041 tacagttggg cattgaatac ggcgatggca gcttggtctg caaaagattg taagaggatg
     2101 cgatcgttgg gggtgaatac gtctggataa ttgcggaaga tgaaaataac gccaatgact
     2161 tgtccatgtg cagcgagagg cagaccagtc ccattgagta accccatgct ggcggtgtag
     2221 gttaattcct ttaacatacg gttgagttcg gtcacatcca actcgcggac cttttcttca
     2281 gcaagaagcg gggttaagta ggagaggaat gccggagcga tgccatgtgc cgcagcgacg
     2341 cgccagccgt cgggaagttt gagcgcgatg atgcccgctt gcccagccaa catttccacc
     2401 gaaacgcgca aaatacgcgc catcaatttt tccaagtcca actcttgcgt taagaggcgt
     2461 gcaatttcca gcaagtaatc tcgctggcgc acacggaagt ctgggagcat ggatggaacg
     2521 atggacatag tttcagggat tgtatcaccc gatttctggg tgtgtgtaag agaaatataa
     2581 tcacctaaag ttgtctaagt gactattatg aattggcttt tttgttaatt ctcggtgttg
     2641 acaccctact aattacctat acaattaatg ttgattaggg atttctaatc gataattgac
     2701 ttttttaccc gctactgttg aatttgactg tgtttccatg tagatgtacg atgcattata
     2761 gcgaagacgg gtgaaccgtc ttcgcttgtt aattaaagac acagtgtatt atctctaagc
     2821 gctgtgagtt tgtattttgt tttgaggtgt tttccatgat ccgttctgtt cgttcaattt
     2881 tgatggcgtt ttttctggca gtatcggtgc tggttgtaca ggtatcgcct gtacaggcgt
     2941 tgacctatcc agcggaaatc aatatgagtt tcacaccgat ttccatcact tctggtggaa
     3001 cttcgcgttt acgtgtgacc atttacaatc cgaattcgaa tgccctaaca ggcgccgcct
     3061 ggttggataa tttaattggc gttcaacccg gactttcaat tgcagccaca ccaaatgtcg
     3121 tgcagacatg tactggtaca gtaacggaca gtggcggcgg tgctgtagac cctggcgata
     3181 cttccattcg tctaacgggg ggcactgtcc ccggacaggt tggcagcaac ccaggtagtt
     3241 gttatgtaga agtgagtgtt acttccacaa cgccggggaa tctcatcaac accatccctg
     3301 cttctgcttt gacctcattc accacggacc ctcaggatac aaacactcca cccgcgcaag
     3361 tcccgatcac taacacgact cctgccagtg ccaccctcaa tgtggttgct gttcaaccgc
     3421 cttcggtgag caagggattt gctccgaata cgatctttgt tgggcaggta agccaactca
     3481 ccattaccat ccgcaacaat gacttgaatg cggctttaac caatactact ttcacagata
     3541 cgttgccagt tggggttgtg gtggcaacac cactgacgac caaccttaca ggctgcggtg
     3601 cggggacctt aacttctacg ctgggtggac cagctctcgc tgctggtgat accgcaattt
     3661 ccttgaatgg cggaaccatt gcccggaatt ccaattgtgt tattactgtc aatgtaactt
     3721 ctgtgctgga aaattcctat accaacacca tccctgcagg acctcccggc tctggttcag
     3781 tacaaaccca acagggcgtt acaaatgctg gacctgcttc ggctccttta aatgtgcagg
     3841 cgttcaatct gagtaaatcg ttttcaactt cgcctattgc agttggcggc gtttcacagt
     3901 tatcaatcgt tatccagaat ccggctaatt ttaattacac aggtgtggag atcactgata
     3961 acttgcccgc cggtttagcg atagcgattc cagtgaatac cggttcaacc ggatgtggat
     4021 cgccgacatt caccgatgct ggtggtgggg cactgggagc aggtgat
//
LOCUS       DAOQFT010000185         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12084_length_4060_cov_7.666916, whole genome shotgun sequence.
ACCESSION   DAOQFT010000185 DAOQFT010000000
VERSION     DAOQFT010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12084_length_4060_cov_7.666916"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..2519)
                     /locus_tag="PLR93_05950"
     CDS             complement(27..2519)
                     /locus_tag="PLR93_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26542.1"
                     /translation="MPLDINLSPLYRVQGQEPTEMPGVLALQPPKNAARGREQDRLIV
                     YLALTGNATITTTEYRKLAEDVANVFYQTPRAVTSALRAASDSLNKVLLDRNMSSSNR
                     GQYTLAWLALAAVRESQSIFSLCGPMHAYWFSANGTRHFFEPTISGKGLGSSQTINIH
                     YAQSDLSPNDMLLFCGRLPNAWVTPLEDAQPSSFDAMRRRLTSITNEDLNAVLIQTTT
                     GAGVINLLRGNMVTKTEAPVEVAPTEIITEAPVQEQPQAIEEPSLEEEPAPQTSTPDL
                     PATYEPESTPEGRAHLLQPSTYSLPPQREETAFPASIPNADPLANLPHRTGPREFPAS
                     IPRAKPRTEAEAPAIEENPIVEKIEPKPEPVQEIKTEKESVPEIKIPEGPREPSPQAK
                     QAAKIVVNLMQGFRTGSASLGERFRNFLPRLLPAEEQSTTSVSANAFMGFMAVLIPLI
                     VVTLAVVVYLRYGRNEQYEAYYNQALQMKQQALALTNPVEQRIAWENVLANLDQAENH
                     NVTSDTMNLRQEAESKRDQLLGISRMQFNPAFTAKLGIDVSRMAATENDLYLLNAANG
                     EVLRAIPAGNGGFELDNTFNCTPGVGNTVGPLVDILALPITNIYSATVLGIDASGNLL
                     YCKPGATPQTGLLPAPDTNWGRITSFILDSGNLYVLDAPSRAVWVYLGKESAFVDRPY
                     FFFGQQTPTQDVIDFLVIGDEMYLLHADGRISNCTYSRVDTNASKCQDPVNKINPFPG
                     YADMDLFATAHFTQILFAAPPDPSLLLLDADNQVVMRFAPRTLELQNQFHPTSGRSNP
                     IPRSPVSAVAVSPDHVLYLAVNGQVYFAPNMP"
     gene            2572..3585
                     /locus_tag="PLR93_05955"
     CDS             2572..3585
                     /locus_tag="PLR93_05955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26543.1"
                     /translation="MDYTLLNDFSQIQAQEWNDLLKESISDTPFLRYEYQQAWWQHRG
                     GGEWKNAQLILVTARENGKLIGIAPLFIAEYENQPALLLNGSIEISDYLDLIVRKEDH
                     TNFLTGLLDFLASSLADNWSKLDWYNLPDDSPTLAALKAEGEKRGWAHLEEVYRPTPR
                     IALNGNFEDYLANIDKKQRHEIRRKMRRAAESELNVRFVIVDQSADIEPELRAFFDLM
                     VQDSNKATFLHPAMREQMTAFLRAAHAGGYLWLGFLEIAGEKTAASLNFDYNGKLWGY
                     NSGVSRSNMELSPGWVLMAHTIQWCCENGRYEFDFMRGDEDYKYRLGGVNRFVMRARL
                     NRK"
     gene            3687..>4060
                     /locus_tag="PLR93_05960"
     CDS             3687..>4060
                     /locus_tag="PLR93_05960"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNH26544.1"
                     /translation="MRDLTNEILELIRRTSSSLPPDVEKRLKESIEKEEPGSAARGAL
                     ETIMKNIELSRSKSTPICQDTGTPIFQVHYPVGWSTIQLKKQIRAAMEEATKKSYMRP
                     NAVDAIYDKNTGNNLGGDDFPY"
BASE COUNT         1019 a          941 c         1055 g         1045 t
ORIGIN      
        1 ctaggcatgg aaacctcctt acaacgttat ggcatgttgg gggcgaaata aacctgaccg
       61 ttgactgcaa gatacaacac atgatcaggg ctaaccgcta cggcgctgac agggctgcgc
      121 ggaatagggt tcgatcttcc agatgtaggg tggaattgat tttgaagttc aagcgtgcgc
      181 ggtgcaaaac gcatcacaac ttgattatct gcatctaata ataacaagga tggatctggc
      241 ggcgctgcaa ataagatctg agtaaagtgt gctgttgcga acaagtccat atcagcatac
      301 ccagggaagg ggttgatctt attaaccggg tcctgacatt tcgaagcgtt cgtatcaacg
      361 cggctatatg tacaatttga aatgcgacca tcggcatgca agagatacat ctcatcgcca
      421 atgacgagga aatcgatcac atcttgtgta ggggtttgtt gaccgaagaa gaagtaggga
      481 cggtcaacaa atgccgattc ttttccaaga tacacccata ctgcacgcga aggcgcatcg
      541 agcacataca gatttccaga gtccaagatg aagctggtaa tgcgtcccca gttggtatcg
      601 ggggcgggta agagaccggt ttgcggagtg gcgcccggtt tgcaatataa aagattgccg
      661 ctcgcatcaa tacccaagac ggttgcgctg taaatattgg tgatgggcaa agcaagaata
      721 tccactaatg gacctactgt attgccaaca ccgggagtac agttgaacgt attgtctaac
      781 tcaaagccac cattcccagc agggatggca cgcagcactt caccatttgc agcattgagc
      841 agatagaggt cattttctgt ggctgccatg cggctgacat caattccaag tttcgcagtg
      901 aacgccgggt tgaattgcat gcgagaaatg cctagcaact ggtcgcgttt ggattcagct
      961 tcctgacgaa gattcatcgt gtcgctggtg acgttgtgat tctcagcctg atccaaatta
     1021 gcaagcacgt tttcccaggc aatgcgttgt tcaactgggt tagtgagtgc cagagcttgt
     1081 tgcttcattt gtaacgcttg gttgtaatag gcttcatact gttcgttgcg tccataacgc
     1141 aggtaaacaa ccacagcaag cgttacaaca atgagcggaa tgagcactgc catgaatccc
     1201 ataaaggcgt tggctgaaac cgaagtggtg ctttgctctt ccgcgggtaa aaggcgcggc
     1261 aaaaaattgc ggaagcgttc accgagtgaa gcgctgccgg tgcgaaatcc ctgcatgagg
     1321 ttgacgacga tcttcgctgc ttgtttggct tgtggcgaag gttcacgcgg accttcagga
     1381 atttttattt caggaacgga ttctttttcg gttttgattt cttgaacagg ttctggcttc
     1441 ggctcgattt tttcgacgat aggattctct tcgattgccg gagcttctgc ttctgttcga
     1501 ggtttggctc tcggaatgga tgccggaaat tcgcgtggac ctgttcggtg tggaagattc
     1561 gctaatgggt ctgcatttgg aatggatgcg gggaatgccg tctcttcacg ttgaggcgga
     1621 agagaatagg tggaaggttg taagagatga gcacggcctt caggtgttga ctcaggttca
     1681 taggtggctg gcaagtctgg cgtcgaagtt tgaggcgcgg gttcttcctc caaacttggt
     1741 tcttctattg cttgaggttg ttcttgaaca ggcgcttctg taatgatttc tgtgggggcg
     1801 acttcaacag gtgcctctgt ttttgtcacc atgttgccgc gcagaaggtt gatgacgcct
     1861 gcaccagttg tggtttgaat aagcaccgca ttcaggtctt cattggtaat cgaagtgagg
     1921 cggcggcgca tggcgtcgaa agaagagggt tgtgcatcct caagcggagt gacccaagcg
     1981 ttggggagac gtccgcaaaa aagcaacata tcgtttgggc ttaggtcact ttgtgcgtaa
     2041 tgaatgttga tagtttggct ggagcccaag cctttgccag aaattgtagg ttcaaagaaa
     2101 tgacgcgtgc catttgcgct aaaccaatac gcatgcatgg gaccacacaa cgaaaagatg
     2161 ctctgacttt ctcgcacagc cgcgagagcc aaccatgcca acgtgtattg accacgattg
     2221 ctagagctca tattacggtc gagcaacact ttattcaacg aatcagaagc ggcgcgcaaa
     2281 gcactggtta cggcacgcgg agtttgatag aacacattcg caacatcttc agcgagtttg
     2341 cgatattcgg ttgtagtgat ggtggcattg ccggtgagcg ccaaatatac gatcagtcgg
     2401 tcttgttcgc gcccacgagc ggcgtttttg ggtggttgca gcgccagtac gccgggcatt
     2461 tcagtgggtt cttgcccctg aacacgatat aaaggtgaaa gattgatgtc gagtggcatg
     2521 taagataatc gcttaagtcg ccttcattat aactggtaaa attcgacggc tatggactat
     2581 acacttctca acgatttctc tcaaattcag gcgcaagaat ggaacgatct tctcaaagag
     2641 tccattagcg acacgccctt tcttcgatac gaataccaac aggcatggtg gcaacaccgt
     2701 ggtggtggtg agtggaagaa tgctcaactg attctcgtca cagctcgtga aaatggcaaa
     2761 ctcattggta ttgctccgct tttcattgca gaatatgaaa accagcctgc tttgctcctc
     2821 aatggcagta ttgaaatttc ggactatctg gatctcatcg tccgcaaaga ggaccataca
     2881 aattttctca cggggctatt agacttcctc gcctcttccc tagccgataa ctggtccaaa
     2941 ctcgattggt ataacctccc tgatgattcg cccactctcg ccgccctcaa agccgaaggc
     3001 gaaaaacgcg gctgggctca tcttgaagaa gtgtatcgcc ctactccacg cattgccctc
     3061 aacggcaact ttgaagatta tctcgccaac atcgacaaaa aacagcgcca tgaaattcgt
     3121 cgcaagatgc gccgcgcggc tgagtctgag ctcaatgttc gctttgtcat cgtagatcaa
     3181 tctgcagata tcgagcctga acttcgcgcc tttttcgact tgatggtgca agactcaaac
     3241 aaagcaacct ttttacaccc tgccatgcgc gagcaaatga ccgccttctt gcgcgctgct
     3301 catgcaggtg gctatctctg gcttggcttc ttagagattg ccggtgaaaa gaccgctgct
     3361 tctttgaact ttgattacaa cggaaaactc tggggctaca actctggtgt tagccgttcg
     3421 aatatggagc tttcgccagg ctgggtgttg atggctcata ccattcaatg gtgttgcgaa
     3481 aacggacgtt atgaattcga cttcatgcgc ggcgatgaag attacaaata ccgcctcggc
     3541 ggcgtaaatc gctttgtgat gcgagccaga cttaatagaa agtaattttt tacggagtcc
     3601 agcgttagaa aatacgcagg gctccgtttt tatttggcaa attccccttt tcgggagtaa
     3661 gattcaattc tgtattggag gtacccatgc gagatttaac caacgaaatt ttagaactca
     3721 ttcggcgcac atccagctct ttgccgcctg atgttgaaaa acgcttgaaa gaatccattg
     3781 aaaaagaaga gccgggttct gcagcacgcg gtgcacttga aaccatcatg aagaacatcg
     3841 agctttcccg ttctaagtcc acgccaatct gtcaggatac gggtacacct atttttcagg
     3901 tgcattaccc ggtgggttgg tctactattc agctcaagaa gcagatccgc gctgcgatgg
     3961 aagaagccac gaagaaatcc tacatgcgtc caaacgcggt ggatgctatc tatgataaga
     4021 acactggcaa caacctcggc ggcgatgatt tcccgtacat
//
LOCUS       DAOQFT010000186         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12103_length_4057_cov_5.408796, whole genome shotgun sequence.
ACCESSION   DAOQFT010000186 DAOQFT010000000
VERSION     DAOQFT010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12103_length_4057_cov_5.408796"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2281
                     /locus_tag="PLR93_05965"
     CDS             <1..2281
                     /locus_tag="PLR93_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26545.1"
                     /translation="IAPTNPSLLVRTPSDMLGKTFHELLPIDIADSFLSKVQEALISD
                     TKVQFEYELAISGQLFWFLATLTKLDENSVFWVARDITERKKSEEAIQRRNQYLAVSA
                     EIGKLVTSILDLSTIFARTVNLITDRFNVYHAAIFIVEETGFNAVLKEGTGEAGAEMK
                     QMEHSLQLNDKSIVGSVASTGQLVVANNVLENPLYMAHPLLAETRSELAIPLKIGTRV
                     IGVIDMQSRELNSFNEDEVSVLQTLADQVAVAIDNARSFELSQQAVMEMREADRLKSQ
                     FLANMSHELRTPLNSIIGFSRVILKGIDGPVTELMQQDLTAIYNSGQHLLGLINDILD
                     SAKIEAGKMELAFDEVNIQDLTHSVLSTMSGLIKDKPIEMRQFIEQDTPTVRADAIRI
                     RQVMINLLSNAAKFTEEGSITVNVAPYREDGKKLVKISVTDTGSGISIQDQEKLFQAF
                     SQVDASPTRKSGGTGLGLSICKQLINMHGGQIWVESKESEGSTFHFTLPLFNKENELA
                     EEGNKVILTIDDDPQVIGLYERYLQPQGYHVHSLTDPSRAVEVAKQVKPFAITLDIMM
                     PGTDGWTVLDTLKSDSATRNIPVIICSIVEDIEKGFNLGASDYLVKPILEEDLVNSLD
                     RLNADGSIREVLVIDDNPNDLRLIGKMLNDDGRYKPILAEGGRNGWTIISSGTPPHAI
                     ILDLFMPEMDGIQILEQLQADKRLRDIPTIVISGMDVTPEQKNKLKEFGQRLLTKGAF
                     NESELLTSIQRSLDRLSKK"
     gene            2304..3959
                     /locus_tag="PLR93_05970"
     CDS             2304..3959
                     /locus_tag="PLR93_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNH26546.1"
                     /translation="MSFIIKCLDCGHNAPYFPTSTTCPKCGSQWREAEYDYEMLGKTL
                     PEKLKSRQQDLWRYRELLPVRNPNISLSLGEGYTPLIRAVNLGMMLGCPNIFIKDERQ
                     GPTSSFKDRQASVTIAALKEAGITEMVAASTGNVAISYSAYASRAGMKLWAFVTSLVP
                     AVKMREIALYGSQVIKITGSYDQCKQVAAEFARQRKLYLDMGARTITSIEAMKTIAFE
                     IAEQLTNVLGPDENSQLRTPDWYVQAISGGMGPIGVHKGFREMQQMGWVDKIPAFAPI
                     QAEGCAPMVDSWKKGLAKAEPVTNPKTRIETLATGDPGRAYEFLKGHVDSSNGVFESV
                     TDEEAFRAMHVLAKMEGISAEPAAGAAFAGLFKLIRAGVIKPSDTVVVNCTGHTMPAE
                     QGILGENWARDVKFPSLAQAPQEEGLLSALTQVAPERFPKIAIVEDTLEARRLIRRIL
                     QSQGNFTILEAEDGRAGLDLVQKELPDLVILDLMMPEMDGFSVIEALRSNPETATIPV
                     IVATAKELTPDEKNRLSGHIQALLQKGDFLNDEFLDEVRSLLK"
BASE COUNT         1138 a          924 c          944 g         1051 t
ORIGIN      
        1 aattgccccc accaacccat ctctcttggt tcgcacccct tcagacatgc tgggaaagac
       61 ctttcatgag cttttgccga tagatattgc cgactcattt ctttcaaagg ttcaagaagc
      121 tttaataagt gatacaaaag ttcagtttga gtatgaactt gctatatctg gacaactttt
      181 ttggttcctc gcgaccctga caaagttaga tgaaaatagc gtcttttggg tggcgagaga
      241 tattacagaa cgcaaaaaat ctgaagaagc gattcaacgc agaaatcagt atttggcggt
      301 atcagctgaa atcggtaaat tggtcacttc tatcctcgac ctgagcacca tctttgcccg
      361 taccgtaaac ctcatcacag atcgtttcaa tgtgtaccat gccgcaattt ttattgtaga
      421 agaaactggc tttaatgcgg ttctgaaaga aggaaccgga gaagctggcg ctgaaatgaa
      481 acaaatggaa cattctttgc aattgaatga caaatccatt gttggctcgg ttgcatctac
      541 tgggcaattg gtggttgcaa ataatgttct tgaaaatccg ctttacatgg cacatccatt
      601 attggcagag acacgttcag agcttgccat tccgctcaag atcggtacac gtgtaatcgg
      661 tgtgattgac atgcaatcac gagaactaaa ctcattcaat gaggatgaag tttcggttct
      721 tcaaaccctt gcagaccaag tggctgttgc tatcgacaat gcccgctcct ttgaactctc
      781 acaacaagcc gttatggaaa tgcgcgaggc agaccggtta aaaagccagt tcctcgcaaa
      841 tatgagccat gaattgcgta cgcctttgaa ctccatcatt ggcttttcac gcgttattct
      901 aaaaggtatt gacggacctg tcactgagtt aatgcagcaa gacctaaccg caatttataa
      961 ctctggtcaa cacttgcttg gtctcatcaa cgatatcctc gactctgcca agatcgaagc
     1021 cggcaaaatg gaattggcgt ttgacgaagt taatattcaa gacctgacac acagtgtatt
     1081 gtctacgatg agcggtttga taaaagataa acccattgag atgcggcagt ttatcgagca
     1141 agatacgcca accgtccgcg ccgatgcgat tcgtatccgt caagtcatga tcaacctgct
     1201 ttctaatgca gctaaattca cagaggaagg ctccatcacg gttaacgttg ctccttatcg
     1261 tgaagatggg aagaaactag tcaaaatcag tgtgaccgat acaggctctg gtatttcaat
     1321 ccaagaccaa gaaaaactct tccaagcctt ttctcaagta gatgcttctc ctacccgcaa
     1381 aagcggtggc acaggcttag gtctttccat ctgtaaacaa ttaatcaata tgcatggtgg
     1441 gcagatctgg gtagaaagta aagaaagtga aggaagtacg ttccacttca ctttgccgct
     1501 gttcaataaa gaaaacgaat tggcagagga aggcaataag gttatcttaa ctattgatga
     1561 tgacccacaa gtgattggtt tgtatgaacg ttacctccaa ccgcaaggct atcacgtcca
     1621 ctcattaaca gatccttcgc gagcagtaga agttgccaaa caagtaaagc cttttgcaat
     1681 tactcttgat attatgatgc ccggcaccga cggctggact gttttggata cgctcaaatc
     1741 cgactctgca acccggaata ttccggttat catatgtagt atcgttgaag atattgaaaa
     1801 aggtttcaac cttggcgctt ccgattactt ggtaaagcct attctagaag aagacttggt
     1861 caattcgtta gatcgcttga atgcggatgg ctccattcgt gaagtgcttg tgattgatga
     1921 caaccccaat gacctgcgtt tgattggcaa aatgctgaat gacgatggac gatataaacc
     1981 catccttgcc gaaggtgggc gcaacggttg gacgattatc tcctctggaa caccgcctca
     2041 cgcgattatc ttggatttgt tcatgccgga aatggacggg attcaaatcc ttgagcagtt
     2101 acaggcagat aagcgtttac gcgatattcc caccattgta atcagcggta tggatgtcac
     2161 gcctgaacaa aagaataagc tcaaagagtt tggtcaacgc ttattaacca agggagcttt
     2221 taacgaaagt gaattactaa cttcgattca acgctcgctg gatcgcttgt caaagaaata
     2281 atcgaagtgt acaggagaat taagtgtcat ttattattaa gtgcctagat tgtgggcata
     2341 acgcgccata ctttccaacc tccacaacct gccccaagtg tggaagtcaa tggcgcgaag
     2401 cggaatacga ctatgaaatg cttggcaaaa ccttgccaga aaagttaaag tcgcgccagc
     2461 aagacctttg gcgatataga gagctacttc ctgttcgcaa cccaaacatt agtttatcgc
     2521 taggagaagg ctacacccca ttgattcgtg ccgttaactt ggggatgatg ttgggctgtc
     2581 caaatatctt tatcaaagat gaacgacaag gacccacttc ttctttcaaa gaccgtcagg
     2641 catcagtgac tattgcggca ctcaaagaag ccggtatcac tgaaatggta gctgcatcga
     2701 ctgggaatgt tgccatttca tattctgcct acgcttctcg agcagggatg aagctttggg
     2761 catttgttac cagccttgtt ccagcagtca aaatgcgtga gatcgctttg tatggcagcc
     2821 aagtcatcaa aatcactggg tcatatgacc aatgcaaaca agtggctgct gaatttgcac
     2881 gtcaacgcaa actgtatctg gacatgggtg cacgtactat cacatccatt gaagcgatga
     2941 agaccattgc atttgaaatc gcagaacagc ttaccaacgt tctcggtccc gatgaaaact
     3001 ctcagctgcg caccccggat tggtatgtgc aagccattag cggcgggatg ggaccgattg
     3061 gcgtccacaa aggctttcgt gaaatgcaac aaatgggctg ggtggacaaa atccccgctt
     3121 ttgctcccat tcaggcagag ggctgtgcgc ccatggtgga tagttggaaa aagggtcttg
     3181 caaaggcaga gcctgttacc aatcccaaaa ctcgcattga aacccttgcc acaggcgacc
     3241 ccggacgtgc ctacgaattt ttgaaagggc atgttgattc aagtaacggt gtttttgaaa
     3301 gtgtgaccga tgaagaagct ttccgtgcta tgcacgtgct cgcgaagatg gaaggtattt
     3361 ccgcagagcc tgccgctggg gctgcgtttg ctggcttgtt taaattaatt cgtgcaggtg
     3421 tgatcaagcc aagtgacacg gtggttgtta actgcactgg gcataccatg ccagctgaac
     3481 aaggtattct tggtgagaac tgggcacgag atgtgaagtt cccgtcttta gctcaggcac
     3541 cacaagaaga aggcttacta tctgcgttaa cgcaagtagc acctgaacgc ttccccaaga
     3601 tcgccatcgt agaagacacg cttgaagcac gccgcttgat tcgccgcatt ttgcaatcgc
     3661 aagggaattt cacaattctt gaagcagaag atgggcgtgc cgggttagac cttgttcaaa
     3721 aagaattgcc cgaccttgtt attctcgact tgatgatgcc agagatggac gggttctctg
     3781 ttatcgaagc attacgttcg aatcctgaaa ccgcaaccat ccctgtgatt gttgcgacgg
     3841 caaaagagtt gacacccgat gaaaagaatc ggctcagcgg acacatccag gcattgctgc
     3901 aaaaaggtga ttttctcaac gacgaatttc tggacgaggt caggtctctc ttaaagtaac
     3961 tattcaataa aaggctggca agattggcaa aaaaaaatac agccgggatc cttgcggcgt
     4021 ttagctccgc ccttttcctt ggcatggcac ctgtgtt
//
LOCUS       DAOQFT010000187         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12118_length_4054_cov_6.624906, whole genome shotgun sequence.
ACCESSION   DAOQFT010000187 DAOQFT010000000
VERSION     DAOQFT010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12118_length_4054_cov_6.624906"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PLR93_05975"
     CDS             <1..1051
                     /locus_tag="PLR93_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNH26547.1"
                     /translation="SKVMMFKTKQSCKTNKILLTITLLFTLGCSFPLQVTFGTPTATP
                     TLTPTPAATQAPLSTPEPGTEQNPLILALAPNPHPNDAIISAGDTLAAFIEKQTGYRV
                     VTVIPASEEALVNDLAKGNAHIASLTPYAFVLAHENGSVNALLARVRNGEMFYGAQIL
                     INRENVFTAYYDEERAENTTDALSALTQFADKKPCWSDETSPSGYVVPLGLLNQYQVQ
                     TRAGAFLEGQPNVVRGVYAADICDFGATFVDARNSPILEADYPDVMDKVRVAWRIPEI
                     IPYENISMAASLPFEMRRSVQRSLIDFMVTADGKAVMQTIYGFDEMLPIEDASYAEFI
                     KYVNASGLNLLDLIE"
     gene            1131..1703
                     /locus_tag="PLR93_05980"
     CDS             1131..1703
                     /locus_tag="PLR93_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNH26548.1"
                     /translation="MPRWIMALFAAPRTFTVEDVPATNVVIVFGAGLLRDGSAGPVLS
                     DRMLTAVNLYHAGKVEKILVSGDNRFIEYNEPEAGRQYALEHGVPDEDIVLDYAGRRT
                     YDTCYRAKHIFGVNEAILVTQDFHMPRALALCNWFGLNAVGVESNNRYFLKRSQIWWN
                     FRETFAVFQAAWDLMVTKPLPVMGEPEPIN"
     gene            1736..2311
                     /locus_tag="PLR93_05985"
     CDS             1736..2311
                     /locus_tag="PLR93_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNH26549.1"
                     /translation="MNREEALALVREYVKSESLVRHMLSVEAAMRFYAEKFGEDVETW
                     GLVGLLHDFDWEIHPTLEEHPQAGAPMLRERGVPEDIIQDILSHADHLNMPRDTMRRK
                     AICACDEVTGLITAVALVRPSRSLYDLEASSVKKKWKDKAFAAGTNREEMEHAAKEFG
                     IDVWEHVGNVIVAMRKIAPELDLVGNISNPS"
     gene            complement(2308..2694)
                     /locus_tag="PLR93_05990"
     CDS             complement(2308..2694)
                     /locus_tag="PLR93_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26550.1"
                     /translation="MEYTIQLDQANGLILVHAAGDWESDKDNLMVQQIMQTVTESGLR
                     KILLDIRELKFDLSMAHIFERAVAMRDTRMKQDKVSSRVALVYDAQNQKLEADMRFFE
                     TASQNRSIPYRVFRDIEEARLWLLQA"
     assembly_gap    2748..2847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2848..>4054)
                     /locus_tag="PLR93_05995"
     CDS             complement(<2848..>4054)
                     /locus_tag="PLR93_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNH26551.1"
                     /translation="VALKNLDEIIELIRSAPDVETARAKLMKRYKLTEIQANAILDMQ
                     LRRLAALERKKIETEYKEVTAIIKELTTLLKTPKLMRGVVADELQKVKAAYGDRRRTQ
                     VVSVKEGGTAKALTTNDLLPQQTVWVGVTDDGLVSRTADDKQPKHSGNDAPRFLVKAS
                     TTDTVYFVSKFGKAAAVAAHLIPEASKLSQGVPWHRVSPLSDNDALAAVFALPASKSA
                     LGEEVCVLTATRFGMIKKSLVTELPGPSSQTFVLVKVNEGDKLNSVGITDGKKDILLV
                     TASGMAIRFKEEDVRPMGLVAAGVNGMKLEDKDEVVGMEILPADGDIFLLTNEGKAKR
                     VDQKDFPVQGRYGKGVVAWDMPGKSTLAAVASGKPNHVATVHMSKGAPKSTRLDAAGL
                     RKRAAGKGDV"
BASE COUNT          960 a         1021 c          933 g         1040 t
ORIGIN      
        1 tagcaaggtc atgatgttca aaaccaaaca atcctgtaag actaacaaga ttctattaac
       61 cataacacta ttgtttacgc ttggatgttc tttcccgttg caagtgacct ttgggactcc
      121 cactgcgact cctactctca cgccaactcc tgccgccaca caagcgcctc tttccacgcc
      181 tgagccgggc actgaacaaa atcctttgat cctcgcctta gctccaaacc cgcaccccaa
      241 cgatgccatc atctctgccg gagatacact cgccgccttc atcgaaaagc agaccggtta
      301 tcgcgtggtg actgtcatcc cggcatcaga agaagcgttg gtaaatgatt tagcaaaagg
      361 taatgcgcat attgcatccc ttacgccgta tgcctttgtg cttgcgcatg agaacggatc
      421 ggtcaacgcc ttactggcaa gagtccgcaa cggagagatg ttctatggcg cgcagattct
      481 tatcaatcgt gagaatgtat ttacagccta ttacgacgaa gaacgggcag agaacactac
      541 cgatgcgctc tctgctttga cgcaatttgc cgataaaaaa ccatgctgga gcgatgaaac
      601 atccccatct gggtatgtgg tgccgctcgg gttgctgaat cagtatcagg tccagaccag
      661 agccggtgct tttcttgaag gtcaacccaa tgtggtgcgt ggagtctacg ctgcagatat
      721 ctgtgacttc ggcgcaacct tcgtcgatgc acgcaactcg cccattctcg aagcagatta
      781 ccccgacgtg atggataagg ttcgagtggc gtggcgcatc ccagagatca ttccatatga
      841 aaatatttca atggctgctt ctttgccttt tgaaatgcgc cgttctgtgc aacgttcttt
      901 gatcgacttt atggtcacag ccgatggcaa agcggttatg caaactattt atggcttcga
      961 cgaaatgctg cctattgaag atgcttcgta tgcggagttc atcaagtacg tgaatgcctc
     1021 gggtttgaat cttttggatt tgatagaata aaacgatgtt taaaagagta atccgtttcc
     1081 tttggcggac tctgcttgtg ctgagtgctc tcaccgccat tggattgttt atgcccagat
     1141 ggattatggc attattcgct gctccacgaa ccttcacagt ggaagatgta cctgcaacca
     1201 atgttgtgat tgtgttcggc gccggactct tgcgcgatgg ctctgccgga ccggtactca
     1261 gtgaccgaat gttgactgcc gttaatttgt atcatgctgg caaagtagaa aaaatcttgg
     1321 tcagtggtga caatcgcttt attgagtaca acgagccgga agcgggtcgt caatatgctt
     1381 tggagcatgg tgtacctgat gaagacattg tgctcgatta tgcaggtagg cgtacctacg
     1441 atacttgtta tcgcgccaag catatcttcg gcgtgaatga agccattctc gtcacacaag
     1501 attttcacat gccgcgcgca ttggcgcttt gtaattggtt tggtcttaat gcagtaggcg
     1561 tagaatcgaa taaccgttac tttctaaaac gttcgcagat ttggtggaat ttccgtgaga
     1621 cctttgcggt ctttcaagcc gcatgggatt tgatggtcac caagccgtta cctgtaatgg
     1681 gtgaaccaga acctataaac taatatcaca tgagtgacaa ccaaaggaga actctatgaa
     1741 tcgcgaagaa gctttagccc ttgtacgtga atatgtcaaa agcgaaagtt tggtacggca
     1801 tatgttatcg gtagaagccg ccatgcgttt ctatgccgaa aagtttggtg aagatgtaga
     1861 gacttggggg ttggttggtt tgcttcacga ttttgattgg gagattcacc caaccttgga
     1921 agaacatcca caagcgggtg cgcccatgct gcgcgaacgc ggtgtgccag aagacattat
     1981 tcaagacatt ttaagccatg cagatcactt aaacatgccg cgcgatacca tgcgccgtaa
     2041 agccatttgt gcctgcgatg aagtgacggg tcttatcacc gccgttgcct tggttcgccc
     2101 ctcccgttct ttgtacgacc ttgaagctag ctctgtcaaa aagaaatgga aagacaaagc
     2161 ctttgctgcc ggaaccaacc gcgaagagat ggaacatgcc gccaaggaat ttggcattga
     2221 cgtgtgggaa cacgttggga atgtgatcgt cgccatgcgc aagatcgcac cagagctgga
     2281 tttagttggg aatatttcaa accccagtta agcttgtaaa agccacaagc gtgcctcttc
     2341 aatatccctg aagacacggt aaggaatcga gcgattctgg gaagccgttt cgaaaaacct
     2401 catatcggct tctagttttt gattttgtgc atcatagacc aatgccaccc tagagctgac
     2461 cttgtcttgt ttcatgcgag tatcacgcat ggcaacagct cgttcaaaaa tatgcgccat
     2521 ggataggtca aatttcagtt ctcgaatatc gagtaatatt ttacgcaatc cgctttcggt
     2581 gactgtctgc ataatctgtt gaaccatcaa attgtcctta tcgctctccc aatcccccgc
     2641 agcatggacc aagatcaatc cattggcttg atccaattga atggtgtatt ccatgatatt
     2701 ctccttgcta cgattataac tagttccaca acaaaactcc catgaagnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnaca tcgcccttgc cagcggcgcg tttacgcaga
     2881 ccagccgcat ccaagcgtgt ggatttcggc gcgcctttgc tcatgtgaac cgtagcaaca
     2941 tggtttggct tcccagaagc cactgccgcc aaggtcgatt tacccggcat atcccaagcc
     3001 actacgccct tgccataacg tccctgcacg gggaagtctt tctgatccac acgtttggct
     3061 ttgccttcgt tggtgagcag gaagatgtca ccatcggcgg gcaaaatttc cataccgacc
     3121 acttcatcct tatcttcgag cttcataccg ttcacgcctg ctgccacaag tcccatcggg
     3181 cgcacatcct cttccttgaa gcggatcgcc atgccgctgg cagtgacgag caagatatct
     3241 ttctttccgt ccgtgatacc aacagaattg agcttatcgc cttcgttgac cttcaccaac
     3301 acaaaggtct gtgacgatgg acccggcaat tcggtgacca gactcttctt gatcatgcca
     3361 aagcgtgtag cggtcaacac gcagacttcc tcacccaacg cagatttgct cgcgggcaag
     3421 gcaaagaccg ccgccagcgc atcattgtca ctcaatggtg aaacacggtg ccatggcacg
     3481 ccctgactca acttactcgc ttcggggatc aagtgcgcag caaccgccgc ggctttcccg
     3541 aatttgctta caaaatacac agtatcggta gtcgatgcct tgaccaggaa tctcggcgca
     3601 tcattaccgg agtgcttagg ctgtttatcg tctgcagtgc gtgagaccaa accatcatcg
     3661 gtcacaccca cccacacagt ttgttgtggc agcaaatcat tcgtagtgag cgctttggca
     3721 gtgccgcctt ctttgacact tacaacctgc gtacggcgac ggtcaccgta tgcggctttt
     3781 actttctgaa gttcatccgc cactacacca cgcatcagtt tgggggtctt caacagcgtg
     3841 gtcaattcct tgatgatcgc ggtcacttct ttatattcag tttcaatctt cttgcgttcg
     3901 agtgcggcaa gacggcgcaa ctgcatatca aggatcgcat tagcttgaat ctctgtcagc
     3961 ttgtaacgct tcatcagttt agcacgcgct gtttccacgt ctggcgccga gcggatgagt
     4021 tcaatgatct catccaaatt tttcaaggcg acga
//
LOCUS       DAOQFT010000188         4045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12160_length_4045_cov_5.379699, whole genome shotgun sequence.
ACCESSION   DAOQFT010000188 DAOQFT010000000
VERSION     DAOQFT010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12160_length_4045_cov_5.379699"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..937)
                     /gene="thrB"
                     /locus_tag="PLR93_06000"
     CDS             complement(44..937)
                     /gene="thrB"
                     /locus_tag="PLR93_06000"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNH26552.1"
                     /translation="MSRMIKIRVPATSANLGPGFDCLGLALDVWNEIAFEPAEQLKYR
                     ASGEGAEKLNKGNRNLLTKAYARLHEFCGKKASGVRIAARNDIPMSSGLGSSAAAIVA
                     GLFGANEMLGKPLHKDELVKLATEMEGHPDNVAPALLGGLVASVMKHEEIITRRYEVP
                     ALTVVIVKPDVEWLTKTARAVLPKSISRADAIFNIGRATLVVDALRTGDLDLLQKVMD
                     DRIHQPYRLRHISGGMAAYKCAKAFGAAALSGAGPSIILFTQPNQAERAKREIQAVFE
                     ERGIDTKGIITSPGKKGVHCI"
     gene            1027..2061
                     /gene="thrC"
                     /locus_tag="PLR93_06005"
     CDS             1027..2061
                     /gene="thrC"
                     /locus_tag="PLR93_06005"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNH26553.1"
                     /translation="MTYQGLIHRYKQYLDLPAHTQIVSLNEGNTPLIPMPRLGSEFGC
                     ELFIKFEGLNPTGSFKDRGMTAAISEAVGRGAKTVICASTGNTAASAAAYAARAGLRS
                     IVLIPEGKVAVGKLAGAIAYGAEVIQIQGSFDDALSLVVEITQKTPIALVNSINPYRI
                     EGQKTSPFEICDELGSAPDWLCLPVGNAGNITSYWAGFKQYGKGLPRVLGVQAEGAAP
                     LVLGHPVDKPETIATAIRIGRPARGEQALEAAHESNGKIMAVSDAQILDAQRILASEG
                     VWVEPASAAGVAGLIAETATGKLDAKGKRVVVVCTGHGMKDPSIITESFSAPQVIPAK
                     YEALVELVGS"
     gene            2106..3749
                     /locus_tag="PLR93_06010"
     CDS             2106..3749
                     /locus_tag="PLR93_06010"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH26554.1"
                     /translation="MSNQPLVIEELTFKYKTRPELALENVSLELKQGELLLIAGSSGC
                     GKTTLARCINGLIPRSYRGDRSGKVLLHGKEVADLQIPEMAQVVGTLLQDPERQIVAS
                     NVFNEVAFGPENLGLPRAEIVSRVDQALKRLNLEYLRERETFNLSGGEKQKVALAGVL
                     TMNPSILLLDEPLASLDPASAHEALAVFRSLADEGKTVILVEHRVEDAIGIKPDRLLY
                     MEEGKIKYLGPITDLPKEIDHTQVKLPAPWVVQRVKALDIEVKTPTQPKVEERGEPLV
                     VFEDVDFQYDEELPLILKGVNLKIYPGDLIAVLGPNGAGKSTLVKHAIGLLKPTKGRV
                     LVEGQDTRKMSVAQIAHALGYVFQSPTHMLYAPTVREELEFGPKNLAYDPLLIPKFVD
                     ESLSTVNMNEFIEYPPLALSFGQQKRTTIAAVLAMRSKILVMDEPTAGQDFANYTHFM
                     NTLCDSSEDAQSILSANFASTLFITHDVDLAVTYANRVLLFGDKHIVADGKPQDVLKD
                     VNQLLKYRVRPTSLLQLNLDLFSKTGRFLPAESLAAYAP"
     gene            3837..>4045
                     /locus_tag="PLR93_06015"
     CDS             3837..>4045
                     /locus_tag="PLR93_06015"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNH26555.1"
                     /translation="MNDKSTWEFGTRQVVYGAIGAALYGVLSWITNIFPLPAAGNITF
                     RPAVAVLVFFGAAYGPWVGLLAGFIG"
BASE COUNT         1000 a          928 c         1038 g         1079 t
ORIGIN      
        1 agtaaaaaat aaaacaaaaa gcggacttgg aaaagtccgc tttttatatg cagtgaacac
       61 cttttttgcc agggctggta atgatccctt ttgtgtctat tccacgttct tcgaatacag
      121 cttggatctc tctcttggcg cgttctgcct gattcggttg cgtaaaaaga ataatagaag
      181 gacctgctcc agataaggca gccgcgccga atgccttggc gcatttgtat gctgccattc
      241 cgccagaaat atgacgcaaa cgatagggct gatggatgcg gtcatccatc actttttgga
      301 ggaggtcaag gtcaccagta cgaagcgcat ccacaaccag tgttgctcga ccgatattaa
      361 aaatagcatc tgcacgcgaa atagactttg gcaaaacagc ccgagccgtt ttagtcaacc
      421 attctacgtc tggtttgacg ataaccacgg ttaatgccgg aacttcgtat ctgcgagtga
      481 tgatttcttc atgcttcatc accgaagcga ccaacccgcc gagcaaagcg ggtgctacat
      541 tatctggatg tccctccatc tctgtcgcaa gctttaccaa ttcatcctta tgaagcggtt
      601 tgccgagcat ttcatttgcg ccaaagagac ctgccacaat cgcggcggcg cttgagccga
      661 gcccgctgct catcggaatg tcatttcgag cggcaatgcg aactccgctt gcttttttgc
      721 cacaaaattc atgcaaacgt gcatacgcct ttgtgagcag gttgcgattc cctttgttta
      781 acttttcagc cccttcaccg cttgcgcgat atttcaattg ttcagcgggc tcaaatgcaa
      841 tttcattcca aacatccaat gccaaaccga ggcaatcaaa accaggacca aggttcgcag
      901 atgtagctgg gacgcggatc tttatcatgc gggacattat ataatacctg cgttgcaaat
      961 gtggaaaaat gtgcactttg taaccgggat gagtataatc gcctcaatct ataactcgga
     1021 gttgatatga cctaccaggg cttaatccat cgctacaaac aataccttga cttacccgcc
     1081 cacacccaga ttgtttcgct aaacgagggg aatactcctc ttattcccat gccgcgcttg
     1141 ggaagtgaat ttggttgtga gttgtttatt aaatttgaag gtttgaatcc gaccggctct
     1201 ttcaaagacc gcggtatgac agctgccatc agtgaggcgg ttggtcgcgg cgcaaagacc
     1261 gttatctgcg cttccactgg caatactgcc gcgtctgcgg ctgcctatgc ggctcgggct
     1321 ggcttgcgct ccattgtgct gattcccgaa ggtaaggtgg cagtagggaa gttggcaggc
     1381 gctattgcct acggtgcgga agtgattcaa attcagggct cttttgatga cgccctatct
     1441 ttggttgtgg agatcaccca aaaaacaccg attgctctag taaactcgat caatccatat
     1501 cgcattgaag gacaaaagac ctcacctttt gaaatttgtg atgaattggg ttccgctcca
     1561 gattggttat gtttgcccgt cggcaatgcg gggaatatca catcttactg ggcgggtttc
     1621 aagcaatatg gaaaaggttt gcctcgagtg cttggcgttc aagctgaggg agccgctccg
     1681 ctcgtgttag gtcaccccgt ggataagccc gaaacaattg ctactgctat tcgtataggt
     1741 agaccggcgc gcggagaaca agcgctagaa gccgcacacg aatccaatgg aaagattatg
     1801 gcagtatctg atgcgcagat tttggatgcg caacgaattc tggcttcgga aggggtgtgg
     1861 gtggaaccag cgtcagcggc gggagtggca ggcttgattg cagaaacagc caccggcaag
     1921 ttggatgcga agggcaagcg tgttgtggtg gtgtgcacgg gtcatggtat gaaagaccca
     1981 tccattatca cggaatcgtt cagtgcgcct caagtcatcc ctgcgaagta cgaagctttg
     2041 gttgaactgg ttggcagtta aaaaatcttt atgaaagcta ggtttcctag taaaatatag
     2101 aagtaatgtc taatcaacct cttgtcatcg aagaactcac gttcaaatat aaaacccgtc
     2161 ctgaattagc cttggagaat gtttctcttg agctgaagca gggcgagttg ttattaattg
     2221 caggctctag cggctgtggc aagaccacgc tggcgcgctg tatcaacggt ttgattccgc
     2281 gtagttatcg tggcgaccgt agtggcaagg ttttgcttca tggtaaagaa gttgccgact
     2341 tgcagattcc tgaaatggcg caggtggtgg ggactctgtt gcaggaccct gaacgtcaga
     2401 tcgttgctag taacgtattc aatgaggttg ccttcggacc cgaaaattta ggtctgcccc
     2461 gtgctgagat tgtgagccgt gtagaccaag ctttgaagcg cctcaatctt gaatatttgc
     2521 gtgagcgcga gacctttaac ttatcgggtg gtgagaaaca gaaggtggca cttgccggcg
     2581 tgttgacgat gaatccatcc attcttttgc tggatgaacc cctcgcctca ctcgacccgg
     2641 catctgctca tgaagcttta gcagttttcc gcagtttggc agatgaaggc aaaactgtga
     2701 tcttggtgga acatcgtgta gaagatgcaa ttggtattaa accagaccgc ttgctttata
     2761 tggaagaagg caaaatcaag tacctgggtc cgatcacaga cttgccaaaa gaaatagacc
     2821 atacacaggt caaactgccg gcgccgtggg tggtgcaacg agtcaaggcg ttggatatcg
     2881 aagtcaagac gccgactcag cctaaggtcg aagagcgagg cgagccttta gtcgtctttg
     2941 aagatgtgga tttccaatat gatgaggaac ttccacttat cttgaaaggt gtcaacttaa
     3001 agatttatcc cggcgatctg atcgcggtgc ttggaccgaa tggggcgggc aaatctactt
     3061 tggtgaagca tgccattggg ttgttgaagc ccacaaaggg gcgtgtactg gttgaagggc
     3121 aagacactcg caagatgagt gtggcacaga ttgcgcatgc gcttgggtat gttttccaaa
     3181 gcccaactca tatgttgtac gcgccgacgg tgcgcgaaga gttggaattt ggacccaaga
     3241 accttgcata tgacccattg cttattccga aatttgttga tgaaagttta tccaccgtca
     3301 acatgaatga atttattgaa tatccgccat tggcattgtc atttggtcag caaaagcgca
     3361 cgaccattgc ggcagtgttg gcgatgcgct cgaaaatcct tgttatggat gagcctacag
     3421 ccgggcagga ttttgcaaat tacactcact tcatgaatac gttgtgcgac tcttctgaag
     3481 atgcgcaatc catcctctct gcaaattttg cctctacttt gttcatcaca catgatgttg
     3541 acttggcagt aacatacgcc aaccgagtgc tgttgtttgg tgataaacac attgtggcag
     3601 atggtaaacc gcaagatgta ttgaaggatg tgaatcagtt gttgaagtat cgcgtgagac
     3661 ctacttcgct tctgcaactt aaccttgatc tgttttcaaa gaccggaaga tttttacccg
     3721 ctgaaagttt ggcggcatat gctccataaa tttccctgca acgtgaaccc gtatgcaggg
     3781 tttatttaaa caaggtcagc gttcgaatca ttcacttttt taaaaggaga gaagtcatga
     3841 acgataaaag cacttgggaa tttggaactc gtcaggtcgt gtacggtgcg atcggcgctg
     3901 cattgtacgg tgtcctttcg tggatcacca atatcttccc tcttcctgca gccggtaaca
     3961 ttaccttccg ccctgcagtg gctgtgttgg tcttcttcgg cgcagcgtat ggtccttggg
     4021 ttggtttgtt ggctggtttc atcgg
//
LOCUS       DAOQFT010000189         4029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12234_length_4029_cov_7.522144, whole genome shotgun sequence.
ACCESSION   DAOQFT010000189 DAOQFT010000000
VERSION     DAOQFT010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12234_length_4029_cov_7.522144"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLR93_06020"
     CDS             <1..186
                     /locus_tag="PLR93_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26556.1"
                     /translation="DTEYAERPLSLVWQGQRYEIAEIFSRWHGPGEKGFCVKTTNGLA
                     FELTYQEISDNWHVQPL"
     gene            196..1374
                     /locus_tag="PLR93_06025"
     CDS             196..1374
                     /locus_tag="PLR93_06025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH26557.1"
                     /translation="MQEVNEVQRLSKRVAGLKPSGIRKFFDIAATMKDVISLGIGEPD
                     FTTPKPILDAGIRSLQNGETHYTSNSGKIELRQGVADNLKKLYNVKYDPANEIIATVG
                     VSEALYLTFTAVLEPGDEVIIPTPCFVSYQAEVILAGGVPVEIPARIENSFTVDPDDI
                     RKAITPRTKIIFIGYPSNPTGAVAPREVMVEIGKIAEEHNLLVVSDEIYDRLVYDFEH
                     VCFPALSDNLWHRTVLLGGFSKDYAMTGWRIGYACAPADLIKGLVRIHQYTIMSAPTT
                     AQDAAIEALKTGEPYVAEMVAEYDRRRRLLVDGLNRLGLTTFEPKGAFYTFPNIRASG
                     MDDETFAEKLLREESVAVVPGNAFGPGGEGFVRMCYATSYEQIEEALRRMERFMSRHG
                     "
     gene            complement(1422..1760)
                     /locus_tag="PLR93_06030"
     CDS             complement(1422..1760)
                     /locus_tag="PLR93_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26558.1"
                     /translation="MPENSRTVWSSDEGDQRKKTQTTTTHSRSLPPQQQTAYLHRESG
                     GRGGKVVSVVKNLVLTEEDMKTLAKKLKQECGTGGTVKDGVIEIQGEHRQRMAEVLTK
                     LGYKVKIAGG"
     gene            complement(1764..2639)
                     /locus_tag="PLR93_06035"
     CDS             complement(1764..2639)
                     /locus_tag="PLR93_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH26559.1"
                     /translation="MIKLTDVTKRYDNTTVVHPLNLQINEGEIVGIIGHNGAGKSTTM
                     KMIAGLVEPTSGEVRVMGQDMWKHNLKVKQRIGYLPEESPLYETMTARQYLLFFSELY
                     QMPRAKALSRIDGLLESLDLPQKDKLTGEFSKGMKRKTAIARTLLHNPDLLILDEPNS
                     GLDPLTSFFIINYLKTLKREGKTILLSAHNLFHVETICDRVAIIKNGRLLVFDTMDSI
                     RSRLGKREYQVIFHSDDKLEYEQLGGNYIFRTHDVDGIARMLETVSAKGWTLVDLSMR
                     ESALEEIYVKLMTNE"
     gene            2723..>4029
                     /locus_tag="PLR93_06040"
     CDS             2723..>4029
                     /locus_tag="PLR93_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH26560.1"
                     /translation="MNQILVIAWREITRLRKRFGGGGSPLTVLILLGVLGFTSYTLRD
                     SVTLGNGMYRVGVSGNVPAINDSRFAVMEVGADEGQALLAEKKIDLLIVDTQVFSRED
                     DKSQFAERAMKQYMEKEELVRVGNSFAEGEAFPLRVGINYLGAGQPSAGNEAGTSAPQ
                     TTKPEEVIIPSLTPPPAPFTQVLVALIYILPITFISIFFTSSFMDEKANRRLTVLLSA
                     PVSPFQIIFGKMLPYAIFALSTTVLIAYFTKANIPLALAIFAPTTLFIFAIYLMVPLF
                     YRTFKDTTFISMLVTTMTTAYLVFPAMFTNSSDLAYISPLALAVKMYRNEPFGWREYL
                     LPSLPMTAVFVFAMFAGTRLLNEEFLMGYKPLTRKVSDAIYLMMAHSKPWFAVMLWSF
                     LVIPLVYMAQVVMLAFATNLPVRLIVGAVLLAAGFIEEVVKTVG"
BASE COUNT          965 a          935 c         1017 g         1112 t
ORIGIN      
        1 gatacagaat atgcggagcg tccgctttcg ttggtgtggc aggggcagcg ctatgaaatt
       61 gcagaaattt tctcgcgctg gcacggaccc ggtgaaaaag gattttgcgt taagaccacg
      121 aatggtctgg cgtttgaatt gacataccag gaaatttcag acaactggca tgtacaacct
      181 ttataaggag gctcaatgca ggaagtaaat gaagttcaaa gactttcaaa acgtgtggcg
      241 ggtttgaagc ccagcgggat tcgaaaattt ttcgatattg ccgcaacgat gaaggatgtg
      301 atctcgctcg gtatcggtga gccagatttc accaccccca agccgattct agatgccggt
      361 attcgttcac tacaaaatgg tgaaacgcat tacacctcga actctggcaa gatcgaattg
      421 cgacaaggtg ttgcagataa cttaaagaaa ctttacaacg tgaagtatga ccccgctaat
      481 gaaatcattg cgacagttgg ggtttctgaa gcgttgtatc ttaccttcac cgctgtgctc
      541 gaaccgggtg atgaagttat catcccgacc ccatgttttg tttcttacca agcggaagtg
      601 atccttgctg gcggcgtgcc tgtggaaatt ccagctcgca ttgagaatag ctttactgtt
      661 gaccccgatg atattcgcaa agcgatcact ccacgcacga agattatctt tattggttat
      721 ccttccaacc ccaccggtgc agtcgctccg cgtgaagtga tggtggagat cggcaagatc
      781 gcggaagaac ataatttgtt ggtggtatca gatgaaattt acgaccgcct cgtgtatgac
      841 tttgaacatg tctgcttccc ggcgttgagt gataatcttt ggcatcgcac tgtattgttg
      901 ggcggtttct caaaagacta tgccatgacc ggttggcgca ttgggtatgc ttgtgctcca
      961 gcagacctga tcaaaggact cgttcgtatt catcaataca ccatcatgtc tgcgccaacg
     1021 actgcgcagg atgccgcgat tgaagcgttg aaaacgggcg aaccctatgt ggcagaaatg
     1081 gttgcagaat atgaccgccg ccgccgttta ttagtggatg gattgaaccg cctcgggctg
     1141 acgacctttg agccgaaggg cgccttttat accttcccca acatccgcgc ctctggcatg
     1201 gacgatgaaa cgtttgccga aaaattattg cgtgaagaaa gtgtggctgt ggttccgggc
     1261 aatgccttcg gacccggagg cgaagggttt gtgcgcatgt gttatgccac ttcgtatgaa
     1321 cagatcgaag aagctctgcg ccgcatggaa cgcttcatga gtcggcacgg ataagtttat
     1381 aaataaaaaa agaggacagg cattgagcct gtcctctttt tttatccgcc agcaattttt
     1441 actttataac caagcttggt caaaacctct gccatgcgtt gacgatgttc gccttgaatt
     1501 tcgatgacgc catctttcac ggttccacct gtgccacatt cttgcttgag tttcttggca
     1561 agggttttca tatcctcttc agtgagcaca aggtttttca ccacagaaac caccttccct
     1621 cccctaccac ccgactcgcg atgtagatag gcggtctgtt gttgcggggg caaagatcta
     1681 gaatgagttg tggtcgtttg agtcttctta cgctggtcgc cttcatcaga tgaccaaaca
     1741 gtgcggctat tttccggcat ggtctactcg ttcgtcatta gtttgacata gatttcttcc
     1801 agtgccgatt cgcgcatgga tagatccacc aaggtccaac ctttggcaga gaccgtttcc
     1861 aacatgcggg caattccatc tacatcgtga gtacggaaaa tataattccc gccaagttgt
     1921 tcatactcta acttatcatc cgaatgaaag atgacttgat attcgcgttt ccccaaccgt
     1981 gaccgaatgg agtccatcgt atcgaagact aacagtcttc cattcttgat gatcgcaact
     2041 cggtcgcaga tggtttcgac atggaagagg ttatgggcgc ttaacaagat ggtcttacct
     2101 tcccgtttga gcgttttgag atagttaata ataaagaagg aagtcagcgg gtcaagaccg
     2161 gaattgggct catcgagaat aagcaagtcg gggttgtgca acagggtgcg ggcgatagcg
     2221 gtcttgcgct tcataccctt ggaaaattcg ccggtcaatt tatctttttg aggcaaatcc
     2281 aacgactcga gcagaccgtc aatgcgagag agggctttgg cgcgcggcat ttggtaaagc
     2341 tctgagaaga agagcaaata ttggcgggcg gtcatcgtct catacagcgg actttcctct
     2401 ggtaaatagc caatacgttg cttgaccttg aggttatgct tccacatatc ctgacccatc
     2461 acacgcactt cgcccgaggt cggttcgacc aaaccagcga tcattttcat ggtggtgctt
     2521 ttgccagccc cattgtgccc gatgattccg acgatctcac cctcattgat ttgtaagttc
     2581 aaggggtgga cgactgtagt attatcgtac cgcttagtga cgtctgtaag cttgatcatg
     2641 atgccaattt ataccaaaca aactcaaggt aaggggtttg cagaagacag aaacttttgc
     2701 ttccttacaa aagtggaaac agatgaatca aatccttgtt attgcatggc gtgaaattac
     2761 ccgtttaaga aaacgatttg ggggtggcgg cagcccgttg accgtgctga ttttgctggg
     2821 cgttctgggc ttcacttcat acactttgcg tgactctgtc acattaggca atggaatgta
     2881 tcgtgtgggc gtatctggaa atgtgcctgc tattaacgac agtcgcttcg ccgtgatgga
     2941 agtgggtgcg gatgagggtc aagccttgct ggcagagaaa aagatcgacc tattgatcgt
     3001 ggatacgcaa gttttttcac gtgaagacga taagtctcag tttgcagagc gggcgatgaa
     3061 gcaatatatg gagaaggaag aacttgtccg tgtagggaat tcgttcgcag aaggagaggc
     3121 gtttccgctg cgagtgggga ttaactacct cggggccggg cagccctcgg cggggaatga
     3181 agcgggaaca tccgcgcctc agactacaaa accagaggaa gtcatcatcc cttcattgac
     3241 tccacctcca gctccgttca cacaagtgtt ggtggcattg atctatattt tgcccatcac
     3301 attcatcagc atctttttca cgagtagttt tatggatgaa aaagcgaatc gccgtttgac
     3361 tgttttactt tctgcaccgg tctctccgtt tcagattatt ttcggcaaga tgctgccata
     3421 cgctattttt gcgctttcca caacagtcct gatcgcttac tttacgaaag caaatattcc
     3481 actagcgctg gcgatctttg caccgacgac cctttttatt tttgccatct atttgatggt
     3541 gccattgttt taccgcacct tcaaagatac gaccttcatt tccatgttgg tgacgaccat
     3601 gacgacggca tatcttgttt ttccggcgat gttcaccaac agcagtgatc tggcatacat
     3661 ttcgccgctg gcattggctg taaaaatgta tcgcaatgaa ccgtttggct ggcgtgaata
     3721 tttactgcct tctttaccga tgaccgccgt gtttgtgttt gccatgtttg cagggacgcg
     3781 cctgctgaac gaagaatttt tgatgggcta caaaccgctc acccgcaagg tcagtgatgc
     3841 gatttatttg atgatggcac attccaagcc ctggtttgcg gtgatgctgt ggagttttct
     3901 cgtcatccct ttggtgtaca tggcgcaagt ggtgatgttg gcattcgcca ccaacctgcc
     3961 cgtgagattg atcgttggtg ctgtgttact tgcggctggt ttcattgaag aagtcgttaa
     4021 aacggttgg
//
LOCUS       DAOQFT010000190         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12334_length_4009_cov_6.201062, whole genome shotgun sequence.
ACCESSION   DAOQFT010000190 DAOQFT010000000
VERSION     DAOQFT010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12334_length_4009_cov_6.201062"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLR93_06045"
     CDS             <1..500
                     /locus_tag="PLR93_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26561.1"
                     /translation="LTGEQIETAWGKDAISSRDAGFMHYLFPSDLPHYQPSLPFTLRQ
                     LTEADAGLMSTLQAVNSPEDVDEAFVEVTHEIAFGCFHGEELVAAASGYERTGFLDLG
                     VLTHPDYRKKGLGKAVVGATCNWANQKGIIAQYRHNALNTSSQNVATSLNFQKYFSSE
                     SFFLR"
     gene            505..1779
                     /locus_tag="PLR93_06050"
     CDS             505..1779
                     /locus_tag="PLR93_06050"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26562.1"
                     /translation="MAYLKTLYNQYPRQFWLMILGIVISTAGGGMVWPFMLIYAGGKL
                     GLPLSAVASLISINAGVGLFASFLAGTLADRIGRKVVMVSSLVMMGVAYLLLIKAETY
                     LQFALLMGMVGLSNPLYQVGADAMLADMIPTQKRTDAYAINRIAYNASFGIGPAIGGF
                     LASTSYALAFYGASTGFFIYGIIWFFFAHETLDKIVREEKASTLAGQAQIQNEAARSD
                     GYIHVFKDKAYMAFVTLFAIGMIAPSILWILLPVYTKTNFNLPEALYGWIPTTNAFMC
                     VFLQYAVSNQTRKFKTLPVASVGMLVYALGAGSVALMTGFTGFWLSMVILTFGELILI
                     PIASKYVADIAPEDLRGRYMSLYWFGWGMSRTLAPLIGGYLNDNISPRAIWIGGLLFG
                     LTSTLGLILLNSLTNTQTALQTRPKIDTTHGD"
     gene            1782..2168
                     /locus_tag="PLR93_06055"
     CDS             1782..2168
                     /locus_tag="PLR93_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH26563.1"
                     /translation="MKQTLALISYVVRDYDEAIAFFTNAMQFDLVEDTPQENKRWVVV
                     RPRGGVGVSLLLARADTPEQQAAVGNQTGGRVFLFLHTDNFKRDYEHMRLHSIEFMEE
                     PRHEAYGTVAVFKDLYGNKWDLLQPK"
     gene            complement(2177..2776)
                     /locus_tag="PLR93_06060"
     CDS             complement(2177..2776)
                     /locus_tag="PLR93_06060"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNH26564.1"
                     /translation="MTNWAQRVTLQGKYVRLEPMGEQHISRLAEIGAGQNFWSFMLYG
                     DMKTEADMRNWVLDILEREKKGGDLPFVVIHLASGKVAGATRYLNIIPKDRGLEVGGT
                     WYGKEFQRTAVNTECKYLLLTHAFEVLKTIRVQIKTDSINLRSQAAIERIGAVKEGVL
                     RNHMILPDGRIRHSVFYSILDSEWVGVKKNLEAMLKKYE"
     gene            complement(2773..3234)
                     /locus_tag="PLR93_06065"
     CDS             complement(2773..3234)
                     /locus_tag="PLR93_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH26565.1"
                     /translation="MIHYLEREQVIPGTMKDIWEFFCNPYNLNTITPPDMNFKIIKGG
                     DRKMHPGQMIEYQVEFIRGIRSLWLTEITHVREGEFFVDEQRIGPYRLWHHEHIFESV
                     NGGVKMIDRVTYVLPFGLLGDIVHAVWVRRRLDSIFDFRTQKTTELFGNLK"
     gene            complement(3231..>4009)
                     /locus_tag="PLR93_06070"
     CDS             complement(3231..>4009)
                     /locus_tag="PLR93_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNH26566.1"
                     /translation="GLGEGSDIRGMNIEYSPSGIHFTAVAKDIRVAVSSNLVGAYNVS
                     NCLAALTAAVYGLGIDSETASKGIASLEGIPGRMERIDMGQNFTAIVDFAHTPNALKV
                     ALEAGRTMTDKRVIAVFGSAGLRDKEKRRMMAETSAELADLTVLTAEDPRIESLNGIL
                     EEMANGMKSKGGREGVNFWRVPDRGEAIKFALTLAQPGDIVLSCGKGHEQSMCFGKTE
                     YLWDDRTAMRAALADYLEVQGPAMPYLPSQDTPEEEWLKG"
BASE COUNT         1018 a         1033 c          867 g         1091 t
ORIGIN      
        1 cattgactgg tgaacaaatt gaaactgcct ggggcaaaga cgccatatct tcacgagatg
       61 cgggcttcat gcattatctc tttccatcag acttacccca ttaccaaccc tcattgcctt
      121 ttacactgcg ccaactgacc gaagcggacg cgggtcttat gtctacttta caggcggtga
      181 actcacccga agatgtcgat gaagcctttg tggaagtgac tcacgaaatt gcattcggct
      241 gttttcacgg cgaagaactt gttgctgctg ccagcggcta cgagcgaaca ggctttctcg
      301 acctcggcgt gctgacacac cctgattacc gtaaaaaagg gctaggcaaa gctgtggttg
      361 gtgcaacttg caattgggca aaccaaaaag ggataatcgc tcaatatcgt cacaacgcgc
      421 ttaataccag ttcacaaaat gtagctacgt ctttgaactt tcaaaaatac tttagttcag
      481 aaagcttttt tctcaggtaa cttcgtggca tacctcaaaa ctttatataa ccagtatcca
      541 cgccagttct ggttgatgat ccttgggatt gtcatcagta ctgcaggcgg cggaatggtc
      601 tggcccttca tgcttatcta tgccggaggc aaactgggtc ttccgctcag tgcggtggcg
      661 tcgctaatct ctattaatgc aggtgtaggt ttattcgcat cttttctcgc cggaacttta
      721 gcagaccgca ttgggcgtaa agtggtgatg gtctccagcc tcgttatgat gggtgtcgcc
      781 tatcttctcc tcatcaaagc agagacttac cttcaatttg cattgctaat gggcatggtt
      841 gggttgtcaa acccgcttta tcaagtgggt gccgatgcca tgttggcaga catgattccc
      901 actcaaaagc gaaccgacgc ctatgccatc aaccgtatcg cctacaatgc ctcgtttggg
      961 attggtccag cgattggtgg gtttcttgcg tccacttcgt atgcgctcgc attttacggg
     1021 gcatctacag gattctttat ctatgggatt atctggtttt tcttcgcgca cgaaacgctt
     1081 gataaaattg tccgcgaaga aaaagcttcc actttagccg ggcaggctca gatccagaat
     1141 gaagcggcgc gatccgatgg gtatatccac gttttcaaag acaaggcata catggcgttc
     1201 gtcactttat ttgcaatcgg tatgatcgcg ccttctatct tatggattct tctaccggta
     1261 tacaccaaaa ccaacttcaa tttacccgaa gcgctatatg gttggattcc aaccacaaac
     1321 gcattcatgt gcgtgttctt gcaatatgcc gtttcgaacc aaacgcgtaa attcaaaacc
     1381 ctaccggttg caagcgttgg catgcttgtg tatgcgcttg gtgccggcag tgtggcgctc
     1441 atgacgggct ttacagggtt ttggctaagc atggtcattc tcacgtttgg cgagctaatt
     1501 ctgatcccaa tcgccagcaa atatgtggca gatatcgcgc cagaagattt acgcggaaga
     1561 tatatgtcac tttattggtt tggctggggc atgtcgcgca cacttgcgcc actgatcggt
     1621 ggctatctga atgacaacat ctcaccacga gcaatttgga ttggaggtct gctcttcggc
     1681 ttgaccagca ccctcggctt gattctactc aacagcctga ccaacaccca aacagcgctt
     1741 caaactcgac ccaaaattga tactacccat ggagattaac tatgaagcaa actcttgcac
     1801 tgatttctta cgttgtccgt gattatgatg aagcaattgc ctttttcacc aatgctatgc
     1861 aatttgattt ggtcgaagat acgccgcaag agaataaacg ctgggtggtc gtccgcccgc
     1921 gtggcggagt tggcgtatcg ctgctgcttg ccagagctga tacacctgaa caacaagccg
     1981 ctgttgggaa tcaaactggc gggcgcgttt ttctattctt gcacaccgat aattttaaaa
     2041 gagactatga gcatatgcgc ttacacagca tagaattcat ggaagagcct cgtcatgaag
     2101 cctatggaac cgtcgccgta ttcaaagatt tgtatggaaa caagtgggat ttgctacaac
     2161 caaaataaaa atattgctat tcgtattttt tcaacatggc ttccaggttt ttctttaccc
     2221 caacccattc cgaatccaag atcgaataga atactgaatg ccgaatgcgc ccatccggca
     2281 aaatcatgtg attacgcaag actccttctt tcaccgcgcc aatgcgttca atggcagcct
     2341 gagaacggag gttgatcgag tctgttttga tctgtacacg aatggtttta agaacttcaa
     2401 aagcatgggt taacaagagg tatttgcatt ctgtgttcac agccgtacgt tggaattctt
     2461 ttccatacca ggtgccacct acttcaagtc cgcgatcttt gggtatgata ttcaggtaac
     2521 gagttgcacc tgccaccttg ccagatgcaa gatgaataac gacaaaaggg agatcaccac
     2581 cttttttctc gcgctccaga atatccaata cccaatttcg catatcggct tcggtcttca
     2641 tatctccata caacatgaag ctccaaaaat tctgcccagc accgatctct gccaaccttg
     2701 atatatgttg ttcgcccatt ggttcgagac gcacatattt cccttgaagc gtgacacgct
     2761 gagcccaatt tgtcatttca ggttcccaaa caattcggta gtcttttggg tacgaaagtc
     2821 aaagattgaa tccaaccgtc ttctcaccca aaccgcatgg acaatatctc ccagcaaccc
     2881 aaatggcaac acataggtta cacggtctat catctttacg ccgccgttca cgctttcaaa
     2941 gatgtgttca tgatgccaca accgatatgg acctattcgt tgttcatcca caaaaaactc
     3001 accttcgcga acatgcgtta tctccgtcag ccataacgaa cgaatgccac ggataaactc
     3061 cacctgatat tcgatcatct gcccggggtg catttttcta tctccgccct taataatctt
     3121 aaagttcata tcaggcggag taatcgtatt cagattgtat gggttacaaa aaaattccca
     3181 gatatctttc atggttccgg gaattacttg ctctcgttct aaataatgaa tcatcccttc
     3241 aaccattctt cttctggtgt atcttgcgaa ggaagataag gcatggcagg tccctgcacc
     3301 tcaagataat ccgcaagagc ggcacgcatg gcagtgcgat catcccataa atactcggtc
     3361 ttgccaaagc acatcgactg ttcgtgcccc tttccacagg aaaggacaat atcgcctggt
     3421 tgggcaagtg tcagcgcaaa cttgatagct tcgccacgat ctggcacacg ccagaagttc
     3481 acaccttcgc gtccgccctt ggacttcata ccgttcgcca tttcctcaag gattccattc
     3541 agcgactcaa tgcgtgggtc ttcagcagtt agcaccgtta agtcagctaa ctctgcagaa
     3601 gtctctgcca tcatacggcg cttttcctta tcacgaagcc ctgccgaacc aaagacagcg
     3661 atgacacgtt tatctgtcat cgttctgccg gcttctaatg caactttcaa tgcattaggt
     3721 gtgtgagcaa aatccacaat ggcagtgaag ttctgcccca tatcaatgcg ctccatacgg
     3781 cctggaatcc cttctagaga agcaatccct ttggaggctg tttcagagtc tataccaaga
     3841 ccgtaaacag ccgcagttag cgcggcaagg caatttgaga cgttatatgc cccaaccaaa
     3901 ttacttgata ccgctacacg aatatctttt gctaccgccg taaagtggat tccagatggg
     3961 ctatattcga tattcatccc gcgaatatcg ctcccttcgc ccagcccat
//
LOCUS       DAOQFT010000191         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12357_length_4006_cov_8.842318, whole genome shotgun sequence.
ACCESSION   DAOQFT010000191 DAOQFT010000000
VERSION     DAOQFT010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12357_length_4006_cov_8.842318"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..855)
                     /locus_tag="PLR93_06075"
     CDS             complement(49..855)
                     /locus_tag="PLR93_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH26567.1"
                     /translation="MNALEPKVDSQAVIKKVYRAFFGPSKYEVKSSDEAILARGKNYR
                     IPFEGGELAVTTWGESGPSILLMHGWGGARAQMTGFVEPLLAAGYRVVAYDQPAHGES
                     DGKMTNVLEISPTMDLVMQREGKFDAIIAHSFGTIITSYALVKRNFPMPSKLIYLGAF
                     NRLLDSLPRFQVLANLPDEVMHGLRNMITENFGKDVLEEIVNENLVKQIHIPALMFHD
                     RSDNVTPVEDSRAIASAWSEAQYIETSGLGHRGALQSAEIHEKIIDFLKA"
     gene            complement(909..1481)
                     /locus_tag="PLR93_06080"
     CDS             complement(909..1481)
                     /locus_tag="PLR93_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH26568.1"
                     /translation="MVKIENSKRTILNLGETLLQDKGFNGFSYANIAAELGVKNAAIH
                     YHYPTKEDLGIAVMQRYRERFKLWINNARVKDLTPEGKLDWFLSIYTNMRADQGKICL
                     VGSMEAEFNSIPEGLQSEVQGLHKELLIWLQSTLTEGRDAGAFAFKGEPADKAAMLLS
                     TLQGALQMARALGAKKFHAVIEQIKLDLLK"
     gene            complement(1542..>4006)
                     /gene="ppc"
                     /locus_tag="PLR93_06085"
     CDS             complement(1542..>4006)
                     /gene="ppc"
                     /locus_tag="PLR93_06085"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNH26569.1"
                     /translation="EEDHRVQMLRKQEQDSFPAPAHESIGEAIEVLKRNGMTSDEMNA
                     LMQSLSIELVLTAHPTEARRRTILSKTERIAAQLDLIARNGLYSYEKQNAHNALSAAI
                     LALWLTDRTRADKLTVTDEVRTGLYFVESYFWNTLPALYHDLELALATHYPDVKPPHH
                     WLKLASWIGGDRDGNPFVKTDITAETLRLHRGLAVEGLRREFQELGRQLSVSSSRILP
                     PPELLEWIEKRRPFPKRVEYIEKRYANEPYRLAIALLSNDLAEASKEEMTTHLLGTHE
                     HQARLDIREVLHVLSLIASVLPESLVQDQLQAVIHKAQIFGLRAARLDIREDSSRFNS
                     ALSEVLRALNIEPNFESLPDDARTELLERLLRDPVPSLAPSLGVTAAAAETWALFKLI
                     GRTNEIYGSDLLGPIVISMTHAAADVLTVLLLAKWGGCKTLPSITPLFEVIQDLKDAP
                     IILEQLFTSDYYRGHLQSHNHEQMVMIGYSDSNKDGGYVMANWSLYQAQEEITRVAAK
                     HNVKLTIFHGRGGTIARGGGPANNAIRAQPAGSINGKFRVTEQGEIIASRYANPGLAH
                     RHLEQIASAVILASAPHKEEPIPAKWRETMETISNAGFRAYRSLVYETPGFIEFWEAA
                     TPLDEIKRLQIGSRPASRAKAGAVNQIRAIPWVFSWMQSRFNLPSWYSLGSGLEAVAD
                     LSLLQEMYDGWLFFRTLLNNSEISLLKADMDISALYVSLVPDQKLANDVFKTIRTEYE
                     RTCGAVLRISRHQNLLELEPLTQQAVQLRNPYVDPLNYIQVETLQRLRKLEDQDGDEA
                     KSLREVMALTINGIAAGLRNTG"
BASE COUNT         1027 a          989 c          951 g         1039 t
ORIGIN      
        1 ttcttacatc aagcctaaaa taaaaaggac ccggctgggt cctttttatt atgcctttaa
       61 gaaatcgatt atcttttcat gaatctctgc cgattgcaat gccccacggt gacccaaccc
      121 acttgtttcg atgtactgtg cctcgctcca tgcggatgca atggcacgac tatcttccac
      181 cggcgtgaca ttatcggacc ggtcatggaa catcagggca ggaatatgaa tctgctttac
      241 cagattttcg ttgacaatct cttccaacac gtctttccca aaattttcgg tgatcatgtt
      301 tctcaacccg tgcatgactt catctggcag gtttgccagc acttgaaaac gcggaagcga
      361 atccagcaag cgattgaatg cacccagata aatcaattta gaaggcatag ggaaattccg
      421 ctttaccaga gcatacgaag taataatggt gccaaatgaa tgagcaataa tggcatcgaa
      481 cttaccttct ctttgcatca ccagatccat agtgggggaa atctcaagca cattggtcat
      541 cttgccatcc gactcgccgt gagcaggttg atcgtatgca accactcgat atcctgccgc
      601 aagaagcggt tcaacaaaac cagtcatttg cgcgcgggca ccgccccaac catgcattaa
      661 taagatggat ggtccgcttt cgccccatgt ggtgacagcc agctcgccac cctcgaaggg
      721 gatgcgataa ttctttcctc gagcaagaat agcttcatcc gatgatttca cctcatattt
      781 gctcggtccg aaaaaggcgc gatacacctt ctttattaca gcttgcgaat caacttttgg
      841 ttctaaagca ttcatagttt ctccttttga aaacttacta cctagtaagt tttattgaca
      901 aaaaaaattt atttgagtaa atctaactta atctgttcga tcacagcgtg gaatttctta
      961 gcgcccaagg cacgcgccat ttgtaatgcg ccttgcaggg tcgataaaag cattgcggct
     1021 ttatctgcag gttctccttt gaatgcaaaa gcgccagcgt ctctgccttc agtcaaagtg
     1081 gactgaagcc atattagcaa ttctttatgt aagccttgaa cttcgctttg taacccttcg
     1141 ggaatggaat tgaattctgc ttccatggaa ccgacaaggc aaatcttgcc ttggtctgca
     1201 cgcatgtttg tgtaaatact taagaaccaa tccagtttcc cctctggcgt caggtctttg
     1261 acgcgggcgt tattgatcca taacttgaaa cgctcgcgat aacgctgcat aaccgcaatc
     1321 cccaaatctt ctttggtagg gtagtgataa tgaatagcgg catttttcac acccaattct
     1381 gcagcgatgt tggcataact aaacccattg aaacctttgt cttgcaataa ggtttcgccc
     1441 aggttcagaa ttgtgcgttt ggagttctcg atcttgacca ttggtaaaaa ttttacttac
     1501 tagttagtaa gttgtcaagc cttccccatt agagtcacaa actatccggt attcctcaga
     1561 ccagcggcga tgccattgat ggtcaacgcc atcacttcgc gcagagactt cgcttcgtct
     1621 ccatcctgat cttctaactt tcgcagccgt tgcaaagtct caacttgaat gtagttcaat
     1681 gggtccacgt atgggttgcg aagttgcaca gcttgttgcg tgagcggctc caattccagc
     1741 aagttttgat ggcggctgat tcgcaataca gcaccgcacg tccgttcgta ttcagtgcga
     1801 atggttttga aaacatcgtt tgcaagtttt tgatcgggca caaggctgac gtataaagca
     1861 gaaatatcca tgtctgcttt gagtaatgaa atctcggaat tattcaacaa ggtgcggaag
     1921 aatagccagc catcatacat ttcttgaagg agtgaaagat cagccaccgc ctccaaaccg
     1981 gacccgaggc tgtaccagct cggtaagttg aatctactct gcatccatga gaaaacccac
     2041 ggaatcgctc gaatctgatt cacagcacct gccttggcac gagatgccgg gcgtgaccca
     2101 atttgcaaac gcttgatttc atccaaaggc gtcgctgctt cccaaaactc gataaagcct
     2161 ggggtttcat acacaaggga gcgataagca cggaaccccg cattcgaaat ggtctccatg
     2221 gtttcgcgcc atttggcagg aatgggttct tccttatgag gagcagatgc aaggattacc
     2281 gcgctggcga tctgctccaa gtgacgatgt gccaaaccag ggttggcata gcgcgaagcg
     2341 atgatctcgc cttgctctgt aacacggaat tttccattga tgctccccgc aggctgagca
     2401 cggatggcat tgttggcagg tccgccaccg cgggcaatgg tgccaccgcg cccgtggaag
     2461 atggtcagct tgacgttatg tttggcagca acacgggtaa tctcttcttg cgcttgatac
     2521 agagaccagt tcgccatgac ataaccacca tctttgttgc tatcggaata accgatcatt
     2581 accatctgtt cgtggttatg agactgcaaa tgtccgcgat aatagtccga cgtgaaaagc
     2641 tgctcaagga tgattggagc atctttgagg tcttgaatca cttcgaaaag cggtgtaata
     2701 gacggtaatg ttttacagcc gccccatttg gcaagcaaaa gcacggtcaa cacatcggca
     2761 gcagcatgag tcatggaaat gacgatgggt cctaacaagt cactgccata aatttcattg
     2821 gtacgaccga tcagtttgaa gagtgcccat gtttcagcag cggcagcagt gacgccaagg
     2881 ctcggggcga gactcggaac cgggtcccgt aagaggcgct ctaataattc tgtgcgtgca
     2941 tcatctggca atgactcaaa attaggttca atgttcagcg cccgcaagac ttcgctcaat
     3001 gctgaattga aacggcttga gtcttcgcga atatccaaac gagcggcacg cagaccaaag
     3061 atctgggctt tgtgaatgac ggcttgcaat tggtcttgca ccaaactttc tggcaagacg
     3121 gatgcgatca aagataaaac gtgcaagacc tcgcgaatat ccaagcgcgc ttgatgctca
     3181 tgcgtgccca gcaaatgagt tgtcatttct tcttttgagg cttcagcaag gtcgttggat
     3241 aacagagcaa tcgcaaggcg atagggttcg tttgcatatc gcttctcgat atattccaca
     3301 cgtttcggaa atgggcgacg cttttcgatc cattccaaca attcaggcgg cggcaaaatg
     3361 cggctggagc tgacgcttaa ctgcctgccc aattcttgaa attcacgtcg caacccttcc
     3421 actgccaaac cgcggtgcag gcgcaaggtc tcggcagtaa tatcggtctt gacgaaggga
     3481 tttccatcgc ggtcgccgcc gatccacgaa gccagtttta gccaatggtg tggcggcttt
     3541 acatcggggt aatgagttgc cagtgcaagt tcaagatcat gataaagagc cggcaaggtg
     3601 ttccaaaaat acgattcaac aaaatacaaa ccagtgcgca cttcatctgt gacggttaat
     3661 ttatcggcgc gggtgcggtc tgtaagccag agtgcaagga tggcggcaga cagcgcgtta
     3721 tgagcatttt gtttttcata cgaatacaac ccatttctgg cgatcaaatc tagctgggct
     3781 gcgatacgct ctgtctttga gaggatggta cgacggcgag cttcagtagg atgtgcagta
     3841 agaaccagct cgatggaaag actctgcatg agagcattca tctcatcgga ggtcatgccg
     3901 tttcgcttga ggacctcaat cgcttcacca atggactcgt gagcgggtgc ggggaaagaa
     3961 tcctgttcct gcttgcgaag catctgcaca cgatggtctt cttctg
//
LOCUS       DAOQFT010000192         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12391_length_3999_cov_8.093306, whole genome shotgun sequence.
ACCESSION   DAOQFT010000192 DAOQFT010000000
VERSION     DAOQFT010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12391_length_3999_cov_8.093306"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLR93_06090"
     CDS             <1..774
                     /locus_tag="PLR93_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNH26570.1"
                     /translation="QAGQQIYDFLWADFADWYVEVAKLELAEGGARAARAAHTLARVF
                     DMTLRLLHPFTPFVTEEIWGHLRTALRESPLAELYKTQREALIVARWPEPREPEGWEA
                     EKTADFELIQEIVRSIRNLRAEKNVAPSKKIAASISAGAKADLLNQQSKVVAALAGLD
                     HAQLAIHSSLKDKPADAAALVVGSVEIYLPLAGMVDLASEKTRLEKELKEAESHIQRL
                     ENLLNGDFANKAPAALIQKERDKLAAYKDTAEKIKAQLK"
     gene            888..1160
                     /locus_tag="PLR93_06095"
     CDS             888..1160
                     /locus_tag="PLR93_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26571.1"
                     /translation="MQTPATPTQYAPCPQCGQSNAKPVNYTWWGGAIGPRMMSHVKCQ
                     SCNTQYNGKTGKSNTQNIIIYNLVVFVVVFCACGGFAVLTSILNNQ"
     gene            1273..1464
                     /locus_tag="PLR93_06100"
     CDS             1273..1464
                     /locus_tag="PLR93_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH26572.1"
                     /translation="MTMKITEDCIACGACLPDCPTESITEGDGITYVIDQATCVECEG
                     HFDSPKCASVCPVECIVKA"
     regulatory      1509..1669
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1841..2617
                     /locus_tag="PLR93_06105"
     CDS             1841..2617
                     /locus_tag="PLR93_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26573.1"
                     /translation="MKPKTLHTLSRWLGIASLLLAIPLWALIVRISGLPKFILPSPAD
                     VWMRFIKALSDGSLLYHTWVTLAEILLGLIVGVCFATIVGYLLAKSRTLETVLSPYLV
                     ASQAIPVVAIAPLLVIWLGDGILSKVIICALIVFFPVLVNTIVGVRAVSASLYDLMNS
                     LRATRWQILWKLELPASLPILFGGLRIGATLSVIGAIVGELVDAQEGLGFLLQLGDFQ
                     YDTSMVFVAVFMLVFLALCLYGVVTLLERRFLKWQQRSNV"
     gene            2654..3631
                     /locus_tag="PLR93_06110"
     CDS             2654..3631
                     /locus_tag="PLR93_06110"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH26574.1"
                     /translation="MKKVVLLMLTLAVALMGCTSSQSTNAAGELRHIKLPMGYIPNIQ
                     YAPFYVAIDKGYFAEEGIEIEFDYSFETDGVALTGAGELPFAVASGEQVLLARSQGLP
                     VVYAFAWYQQFPISIISAPELNINEPADLRGKKIGLPGLFGANYIGLEALLFEGGVDP
                     SEVSMDAIGFNQVEAYASGNSDIVVGYAANEPIVLASQGFDLKEMRVADYVDLIANGI
                     VTNEDTIANDPELVRGMAKALARGIEATINNPEEAYDISLKFVENLKDQDKAVQMQVL
                     NTSIEFWKAERTGYSNPQAWENMNNVLVKMALIPEPIDLSKAFTNEFIP"
     gene            3687..>3999
                     /locus_tag="PLR93_06115"
     CDS             3687..>3999
                     /locus_tag="PLR93_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH26575.1"
                     /translation="MTTKPILSVRNLSVTYPDNNGGLHALDNISFEVRPREFVCFLGP
                     SGSGKTTFLRVLAGILEPTAGNVNFMYHHQQKIGMVFQQANLMPWRTVIQNIMLPLEL
                     EG"
BASE COUNT         1024 a         1017 c          923 g         1035 t
ORIGIN      
        1 caagccggtc aacagatcta cgacttcctc tgggcagact ttgcagactg gtatgtggaa
       61 gtggcaaaac ttgagcttgc agaaggcgga gcccgtgccg cccgcgcggc gcacacgctt
      121 gcacgcgttt tcgatatgac cctgcgtttg ttgcatccct tcaccccatt cgtgaccgaa
      181 gaaatttggg gacatctccg caccgctttg cgagaatctc cgttggcaga gttgtacaag
      241 actcagcgag aagcattgat cgtggcacgc tggcccgaac cgcgcgaacc cgaaggctgg
      301 gaagcagaaa aaaccgccga ctttgagttg atccaagaga ttgtgcgctc catccgcaac
      361 ctacgtgcag aaaagaatgt ggcaccttcc aagaagatcg ccgcatccat ctctgccggc
      421 gccaaagcgg acttgctcaa tcaacagtcg aaagttgttg ccgctcttgc tggtcttgac
      481 cacgcacaac tcgcgattca ctcatccctg aaagacaagc ccgccgatgc cgccgccctc
      541 gtggttggct ccgtggagat ctacctccca ctcgcgggca tggtagacct tgccagtgaa
      601 aagacccgcc tcgaaaaaga actcaaggaa gccgaatctc acattcagcg tttggaaaat
      661 ctgctcaatg gtgatttcgc caacaaagct cctgccgcgc tcatccaaaa agagcgcgat
      721 aaacttgccg catacaaaga cacagcagag aagatcaaag cacaattgaa gtaataaaat
      781 gaggtcacgg ttgaaccgtg acctcatttt ttcataccaa tggttgattg ctcaatctct
      841 cttgtcttat aaaataaggc tatatcttac actcaggaga aaatctcatg caaaccccag
      901 ctaccccaac ccaatacgcc ccctgccccc aatgtggaca gtccaatgca aaaccggtca
      961 actacacctg gtggggcggt gccattggtc cgagaatgat gagccatgtc aaatgccaaa
     1021 gctgcaatac acaatacaat ggcaagaccg gcaaatccaa tacccaaaat attatcatct
     1081 acaatcttgt cgtattcgtg gttgttttct gcgcatgtgg tgggtttgca gttttaactt
     1141 ccatcctcaa caatcaataa gcaacgcatg acaaactctc catgaattgg agatgttcgt
     1201 cactaaaact tctgtgatgc ttcatgtaca gttacaaact gcaagaaaaa aatccattca
     1261 ggagtaactc ccatgaccat gaagatcaca gaagattgca tcgcctgcgg cgcatgcctg
     1321 cccgattgcc ccaccgaatc catcaccgaa ggcgatggca tcacctacgt catcgaccaa
     1381 gccacttgtg tcgaatgcga aggtcacttc gactcaccca agtgcgcctc cgtttgcccg
     1441 gtggaatgca tcgtcaaagc ctaattgaat cctaatcttg ttgagggtat aatcacctca
     1501 actgaatatt caaccttcgg ggcagggtga cacgcaaccc gcgtgaattc ccgatcggtg
     1561 gtacagccca cgagcctgtt taggtacatc gctttcctca ttgggaaaac gggcatgatc
     1621 cggcgcaact ccggagccga cagtatagtc tggatataag aaggtataag cacaacccct
     1681 tgtgggatct gtgtggattc tcaccccgaa aacgctcttt gttttcgggg tttttattta
     1741 tggaattcac gcgcctcacg cctggattgt tgcctgtttt cacgccccgg agttttatgt
     1801 actctgaggc gtttttattt gccacctgtg actgatgatc atgaaaccaa aaactctgca
     1861 caccttatct cgctggctag ggattgcctc ccttctgctt gcgattccgc tctgggcgct
     1921 tatcgttcgc attagcggtc ttcccaaatt catcctgccc tcccccgctg atgtatggat
     1981 gcgtttcatt aaagcactga gtgacggcag tttgctttat cacacatggg ttacgctggc
     2041 agaaattttg ctgggtttga tcgttggcgt ttgttttgcc acgatcgttg ggtatctcct
     2101 cgctaaatcg cggacacttg aaactgtcct ttcaccgtat ttagtagcga gtcaggcaat
     2161 tcccgttgta gccattgctc ccttattagt catctggctc ggagatggca ttctctcaaa
     2221 agttatcatc tgtgccctaa tcgtattctt tccagtgttg gtcaatacga ttgtcggggt
     2281 acgagccgta tccgcttccc tatatgactt gatgaactcc ctccgtgcta cccgttggca
     2341 aatcttatgg aagctcgaat tgccagcctc gcttcccatc ctctttggcg gacttcgcat
     2401 cggcgcaacg ttatcagtca ttggtgcaat tgtcggtgag ctcgttgatg cgcaagaagg
     2461 tctgggcttc ctgttacaac tcggcgattt tcaatacgac acgtccatgg tctttgtagc
     2521 agtctttatg ttggtctttc tcgcgctgtg cttgtatggc gttgtcaccc tcttagaaag
     2581 aagatttttg aaatggcaac aacgttccaa cgtataaacg tttgaacttg aaaacatctt
     2641 aggagacatt gtaatgaaga aagtagtttt actcatgcta acgctggcag tcgctctaat
     2701 gggctgtacc agctcacagt ccacgaatgc ggcgggggaa cttcgtcata ttaaattgcc
     2761 gatgggctat attcccaaca tccaatatgc accattctat gtagcaattg ataaagggta
     2821 ttttgcagaa gaaggaattg aaatagagtt cgattattct ttcgagaccg atggcgttgc
     2881 attgacgggt gcaggcgaac ttccattcgc agtggcatcg ggcgaacaag ttttattggc
     2941 acgttcacaa gggttaccgg ttgtgtatgc ttttgcttgg taccagcaat ttcccatctc
     3001 aatcatctct gcaccagagt tgaacatcaa tgaacccgca gacctgcgcg gaaagaaaat
     3061 tggcttacca ggtttgttcg gcgcaaacta cattggtttg gaagcattgt tattcgaagg
     3121 cggagtagac ccatctgaag taagtatgga tgccattggt ttcaaccaag tggaagctta
     3181 tgcttcgggc aatagtgaca ttgtcgttgg ctacgcagcg aacgaaccga tcgtattagc
     3241 atctcaaggt tttgacttaa aagaaatgcg tgtggcagat tatgttgact tgatcgcaaa
     3301 tggcattgtg accaatgaag ataccatcgc gaacgaccct gaacttgtgc gcggcatggc
     3361 aaaagcactg gcacgcggca ttgaagctac gatcaacaac cctgaagaag cctatgacat
     3421 cagcttgaag tttgtagaga atctaaaaga ccaagataag gctgtgcaaa tgcaagttct
     3481 aaacacatcg attgaatttt ggaaggcaga acgcacaggc tattccaacc cacaggcgtg
     3541 ggaaaatatg aacaacgtcc ttgtaaagat ggcgttgatt ccagagccta ttgatctcag
     3601 caaagcattt acaaacgaat tcatcccata aggattttac gagtcgcggt tcacacatgt
     3661 agtgatgtgt gaaccgcgaa ttagccatga ccacaaaacc catcctcagt gttcgcaacc
     3721 tttctgtcac ttaccccgat aacaatggcg ggcttcacgc acttgataac atctcgttcg
     3781 aagttcgtcc gcgtgaattt gtttgttttc tgggtccttc cggctcgggg aaaacgacct
     3841 tcttacgcgt gttagcaggc attctcgagc caaccgctgg caacgtaaat tttatgtatc
     3901 accaccagca aaaaatcggc atggtttttc agcaagccaa tcttatgcct tggcgcactg
     3961 tgatccaaaa tatcatgctg cctcttgagt tggaaggaa
//
LOCUS       DAOQFT010000193         3995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12415_length_3995_cov_6.287817, whole genome shotgun sequence.
ACCESSION   DAOQFT010000193 DAOQFT010000000
VERSION     DAOQFT010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12415_length_3995_cov_6.287817"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLR93_06120"
     CDS             <1..1002
                     /locus_tag="PLR93_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26576.1"
                     /translation="PEGGLVYDDAAAIETGVDGVTAVVVEQNSTETVKAGDISLEDVE
                     ILGTTIGFPSVRDMEIADGRYFNEQENERKSKVAVLGYTVAQELFGDTNPVGQYIKIG
                     NTKVAVIGVFKEKGLVGNTDFDSRIYLPIKLVFQKFTPSMFARFMGDSVRMIYVEVDP
                     DANQDEIITQIELLLAKRHDVSLDSADFTVTTQQDIISTQESTTSAFRSLLAWVAGVS
                     LIVGGIGIMNIMLVSVTERTREIGIRQSIGATPEDIRLQFLTEALLLSIVGGLIGVIV
                     GVGGAYIFGSLSDMRTVISLPSILLAFGSSAIVGAFFGYYPANQASKLDPIEALRYE"
     gene            1127..1675
                     /locus_tag="PLR93_06125"
     CDS             1127..1675
                     /locus_tag="PLR93_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26577.1"
                     /translation="MSTITKLAIGTLKLEGTENAITPEQASDLLPLWQVYLSLVDSDT
                     AAQAEIDGLVDQISATLTDSQNQAIEDMQLSQPDMMSVMQEMGISMGNRPQSASDSEQ
                     SGSSGGFPGGGDGFAPPDGGGMPGGGMPSGDMPSGGNMPTLSEDQIATAQASGGAPGL
                     GSSIPTPLIEAVIEYLQKTASS"
     gene            1705..3144
                     /locus_tag="PLR93_06130"
     CDS             1705..3144
                     /locus_tag="PLR93_06130"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH26578.1"
                     /translation="MNVIHWFPKIPIKAKVGIAIASLIAISAGGYSVFSNQSAAAESK
                     ETALQTATIGRGDLSISASGTGILATEDEVELSFSTSGTVTKVYVHAGDEVQAGDILA
                     EIDNTQAQADYSSAEQTYMELTSPKAIASSLETLASAEKSYSDAIYQLEYLVSPDVLY
                     WDQNIEKAKQNLESAQSQLSSDPENSQVKQRIQELEAYLQSASELRSEALNDYYNEYV
                     FENFVRRDSEGHEYLALPTETDITLAQFAVDDAQRKLEEAQTVYTALSSGIIPTNTGI
                     TSLEEVLSAKRALDEAKSILDGTTIIAPKAGMIVTVDIQTGDKVDTKTCITLTDLLQP
                     HLEIYLDESDWDKIEVGYPVEVEFDALPDTLFTGTVDTVDSELSTSNMSSSVHGTVVL
                     DATFAELNLPLGASASVEVTSIDLKNILLVPLDALEESDTGAYTVRVLKNNEVKLQAV
                     DIGIHNELYAEIKSGLNEGDIVVTGTFEE"
     gene            3148..3822
                     /locus_tag="PLR93_06135"
     CDS             3148..3822
                     /locus_tag="PLR93_06135"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNH26579.1"
                     /translation="MRHENKLNKNLLIGLILVISLSACAPAATEPSENEGPTATSTFD
                     SAIATAVANAFTAAAAVTTNTPTQTSTPAFTATALPTFTFTLAPATETPQPAPPTVLS
                     DYDFCDNSAYVSDITIKDGTVLMPGETFKKIWRLQNNGTCPWRPEYTVTFVSGYNMDG
                     VTTTINQYVAPGKSAKITVAMKAPTAEGTYTGYWSLTNFDGVNFGEVVYVRVVVSKDE
                     TEEEPE"
BASE COUNT         1159 a          948 c          907 g          981 t
ORIGIN      
        1 cccgaaggtg gactcgtcta tgacgatgcg gctgccatcg aaaccggtgt agacggtgtg
       61 accgccgttg tggtggaaca aaactccact gaaactgtaa aagccggcga catctctctt
      121 gaagatgtgg aaatcctcgg caccacgatt ggctttccat cggtccgtga tatggaaatt
      181 gccgatgggc gttacttcaa cgaacaggaa aatgaacgca aaagcaaagt agctgtactt
      241 ggttacaccg tagcacaaga attatttgga gatacaaacc ccgttgggca atacatcaag
      301 attggaaaca ccaaagtcgc cgtaatcgga gtgttcaaag aaaaagggtt ggtaggaaat
      361 accgattttg attcacgcat ctacctcccg atcaaactag tcttccaaaa atttacgccc
      421 tccatgttcg cccgatttat gggtgatagc gtccgcatga tttatgttga agtggaccca
      481 gatgccaatc aagacgagat cattacccag atcgaactgt tattagccaa acggcacgac
      541 gtttctctag actcagcaga ttttactgtc acaacacagc aagacatcat ctcaactcaa
      601 gagtcaacaa cctcagcctt tcggtcactt ttggcttggg tggcgggcgt ttctctgatc
      661 gttggcggca tcggcatcat gaacattatg ctggtcagtg tcaccgaacg gacgcgtgag
      721 atcggcattc ggcaatcaat tggcgccacc ccagaagata tccgcttgca gtttttgacg
      781 gaggccctat tgctcagcat tgtcggcggt ctgatcggag tcatcgtagg cgttggtgga
      841 gcatacatct tcggctcttt aagcgacatg cgaactgtga tttccctccc gtccattctc
      901 ctagcgtttg gctcttctgc cattgtcggc gcatttttcg gatattaccc agccaaccaa
      961 gcttcaaaac tcgaccccat tgaagcgttg cgatatgaat aaaggaaatt ttatgaaaaa
     1021 gatatttgta atttcattga tgctatgttc attagctctt tctgcttgta gcagttccgc
     1081 atcaacccca acaggtggaa tgccctcttc taacgacgga ggtgacatga gcaccatcac
     1141 caaacttgct attggaacac tcaaactaga aggaaccgag aatgccatca cgcctgagca
     1201 agccagtgac cttttgcctc tttggcaagt ctatctctca ctagtagaca gcgatactgc
     1261 agctcaagca gaaatcgatg gactggttga ccagattagc gcaacactga ccgattccca
     1321 aaaccaagcg attgaagaca tgcaactttc tcagccagac atgatgtctg tgatgcaaga
     1381 aatgggcatt tccatgggaa accgtccaca aagcgctagt gatagcgaac aaagcggaag
     1441 ctcgggaggc tttcccggtg gtggcgatgg gtttgctcca cctgatggtg gtggtatgcc
     1501 cggtggtggc atgcccagcg gagacatgcc gagcggcgga aatatgccaa ccctaagcga
     1561 agatcaaatt gccactgcac aagcttctgg aggcgctccc ggcttgggaa gtagcatacc
     1621 aactcctttg atcgaagccg tgatcgaata ccttcaaaag acagccagtt catagtgttt
     1681 gaaccgccgt agaaatgata tgggatgaac gtaatacatt ggtttccaaa aattcctata
     1741 aaagctaaag ttggcattgc tatcgccagt cttatcgcaa tcagcgcggg tggatattct
     1801 gtgtttagca atcaatccgc cgctgctgaa agcaaagaaa ctgcattaca gaccgctacc
     1861 atcggtcgcg gagacttaag catttccgct agtggcactg gaattttagc aacagaagat
     1921 gaagtggagt tatcgtttag taccagcgga acggtcacaa aggtgtacgt ccatgctggg
     1981 gatgaagttc aggcaggcga tatactagcc gagattgaca acactcaagc tcaagcagat
     2041 tactcaagtg cagagcaaac gtacatggag ttaacctccc caaaagcgat tgcatcttcc
     2101 ctagaaacat tggcaagcgc ggaaaaatca tactcagatg ctatttatca gcttgaatat
     2161 cttgtcagcc cagatgtact gtattgggat caaaacatcg aaaaagcaaa gcaaaatctg
     2221 gaaagtgccc aaagtcaatt atcaagcgac ccagaaaact ctcaagtaaa acagcgaatt
     2281 caagaattag aagcctactt acaatctgca agtgagctta gatctgaggc tttaaatgat
     2341 tattacaatg aatatgtctt cgaaaatttt gtccgccgcg atagcgaagg gcacgaatat
     2401 ttagcattgc caacagaaac agatatcact ctcgcgcaat ttgcagttga tgacgcacaa
     2461 cgaaagctag aagaagcaca aaccgtttac acagccttat ctagcggaat aattcctact
     2521 aatacaggaa ttacaagcct tgaagaagtc ctttcggcaa agcgggcgct tgatgaagca
     2581 aaatccattt tagatggaac aacgatcatc gccccaaaag cagggatgat tgttactgtg
     2641 gatattcaaa ctggcgacaa agtggacaca aaaacttgca tcacccttac agatttatta
     2701 caaccgcact tggaaatcta cttggatgag tcagattggg ataagataga ggtcggttat
     2761 ccggtagaag tcgaattcga cgccctgccg gatactctat ttaccggcac cgtagacaca
     2821 gtagatagcg agctgtccac cagcaatatg tcttcttcgg ttcatggtac agtagttctg
     2881 gatgccacct tcgcagagtt aaatctaccc cttggagcat ctgcttcggt tgaagtaaca
     2941 agcattgacc tgaaaaatat tttgctcgtc ccacttgatg cgctggaaga atcggatact
     3001 ggagcatata cagtccgtgt cctaaaaaat aatgaagtaa aattgcaagc tgttgatatt
     3061 ggtattcata acgagttgta cgccgaaatc aaatccggtt taaacgaagg tgatattgtc
     3121 gttaccggca cattcgaaga gtagatcatg cgccatgaaa acaagctaaa caaaaatctt
     3181 cttatcggtc tcattttggt catcagtttg tctgcttgtg cgccagcagc cacagagccc
     3241 agtgaaaacg agggtcccac agccacaagc accttcgact cagccattgc aactgcggtt
     3301 gcaaacgctt ttaccgctgc tgctgccgta acaaccaata ctcctaccca aacatcaacg
     3361 ccggcattta cagccaccgc tttacctact tttacattta cgcttgcccc tgcgacggaa
     3421 acgcctcaac ccgcgccgcc aacggttctt agtgattatg atttttgcga caactcagcc
     3481 tatgtgagtg acatcactat caaagatggg accgtgctta tgccggggga aactttcaaa
     3541 aagatatggc gtcttcagaa taacggcacc tgcccatggc gcccagaata tacagtcacc
     3601 tttgtaagcg gatacaacat ggacggtgta accaccacca ttaaccaata tgtagcccca
     3661 ggaaaatcag ccaagattac agttgccatg aaagccccaa ctgccgaagg gacatatacc
     3721 ggatactggt cattgactaa ttttgatggc gtcaattttg gtgaagtcgt ttatgtgcgt
     3781 gtggtcgtgt ctaaggacga aacagaagaa gaacccgaat aacgttattc gtacaatata
     3841 agaagcgctt ccattctatg gaagcgcttc tcttgtttaa gagttggaat tacttattga
     3901 agtggtttta gttcaccacc tccaacaagg gtataggtaa taccattcaa agctttacag
     3961 tttgcggcat cataatatac ggtgtgaccg ttccc
//
LOCUS       DAOQFT010000194         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12568_length_3966_cov_7.241115, whole genome shotgun sequence.
ACCESSION   DAOQFT010000194 DAOQFT010000000
VERSION     DAOQFT010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12568_length_3966_cov_7.241115"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1708)
                     /locus_tag="PLR93_06140"
     CDS             complement(152..1708)
                     /locus_tag="PLR93_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNH26580.1"
                     /translation="MARRAVIYIRTSSETQGANSSPVEQEADCRRLAEEKGLQVAHIY
                     RDVEKYRVGSKLVEPSGSRSDRPGLLAMLKDAARDEFDVILAWREDRLYRGLRSMLTV
                     LETVQDYKIEILLAKENFDSRIAPVRAWAAQMELDGMKERLGMGVKARLKAGKANTGQ
                     DRYGYIRISDKIHVVEEEADWVRKIFDWYNQGVSLLQIRERLIAGNAPQKGSSTPRHI
                     HWARSSIQGILESAKEYAYGHKEQSRDGETFQIPVEPILEIPTYELFKTQREKNKTNS
                     PQHIRHDYLLSGHLKCSCNLTWQAKTATHRRSRQGEWIKRKTPIGTYFCPQTHKELRA
                     SDCPKSISAKQAEAQIWEKVSEFITNPAYLLAEAEAKVSWLQKDYEQMQREELRLQKE
                     LKRLNDERQEFITIACKEQMPDEELTSRMNVLYEKERGVQRRLTTIERTKDDFIKLDL
                     EEQIKKYVAELQSEMVELIHADPQTPEERHQVFLLKKRVVDTVLEEARIDQSREIHIK
                     FRSDFTTRTN"
     gene            1895..3202
                     /locus_tag="PLR93_06145"
     CDS             1895..3202
                     /locus_tag="PLR93_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH26581.1"
                     /translation="MVYYSCMNENIWKSRWSKDQIKSMLLEQFDSFWSRDLGIVRTQL
                     AEVERASGVPHAVIISGLRRAGKSTLLLQLAHRLGREQFYYLNFEDDRFLGFLAEDAN
                     DLYQALLEVFGERKIFIIDEIQNIDGWEHFVRRFMDMGFKFYITGSNASLLSRELGTR
                     LTGRYVPIELFPFSFKEFMLFRGESLPNLERMTTVDHARLQNSLQSYLKFGGIPDALK
                     YPELPLLRTLYDDVLYRDIATRYRLEAVTAIKELGFFLMSNPANLISFNKLKDQFRVG
                     SVNTIKNYIGYMENSWLLFTLNVYDYSVKRQQIAPKKIYGIDTGLINTIGFGFSPNTG
                     KLLENLVFLALRRKAKEIHYLSSPGGYEVDFYLPEQRQLIQVSGNLAQSTTRDREVRA
                     LKDAIGTVKVQSAIILSDSNEDSFEINGIPIHVQSTAEWLLAE"
     gene            3514..>3966
                     /locus_tag="PLR93_06150"
     CDS             3514..>3966
                     /locus_tag="PLR93_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26582.1"
                     /translation="MSYNGFMPTPILATKLFIPPLRTNIVPRPHLTERMKEGKFHKLT
                     LISAPAGFGKTTLVSEWITGCGLPVAWLSLDEGDNDHKRFLAYLIAALRTITKNIGER
                     ASDLLDAAPFPDIESVLTSLLNEITATSDHFILVLDDYHLIESKQVDEA"
BASE COUNT          957 a          973 c          903 g         1133 t
ORIGIN      
        1 tgtttacggg tgttcgactc aaatcccata aacgatcatg ccccactccc ctgcctttca
       61 gtgcagggga gtgttttttt aatgacaaca aggcggctcg aaagtgagcc gccttgttgt
      121 cattttcgga atcagattat ggaggtcaat atcaatttgt tcgggttgta aagtctgaac
      181 ggaatttaat gtgtatctcc cggctttgat caattcgtgc ttcctccaaa acggtatcca
      241 ccactcgctt cttcaacaga aacacctgat gtctctcctc tggggtttgt ggatcagcat
      301 ggatcaactc aaccatctct gactgcaatt cagccacata tttcttgatc tgttcttcca
      361 agtcaagttt tataaaatca tccttcgttc gctcaatggt tgtcagcctg cgctgcactc
      421 ctcgctcctt ctcatacagt acgttcatcc gggaagtgag ctcttcatca ggcatttgtt
      481 ctttacaagc tatggttatg aattcctgac gttcatcatt tagccttttg agttcctttt
      541 gcaggcgtag ctcctctcgc tgcatttgtt cgtagtcttt ctgcaaccag gaaactttag
      601 cctccgcttc agccagaagg taagctgggt tggtaataaa ttcgcttacc ttctcccaga
      661 tttgggcttc ggcttgtttg gcactgatcg acttgggaca atctgaggct cgtaactcct
      721 tgtgcgtctg cgggcagaaa taggtcccaa tgggcgtttt gcgcttgatc cactccccct
      781 gcctgctacg acggtgggtt gccgtttttg cctgccaggt cagattacat gagcatttta
      841 gatgtccaga aagtagatag tcatggcgga tatgttgggg tgaattggtt ttgttctttt
      901 ctcgttgcgt tttgaacaac tcataggtgg ggatttcgag aattggctcc accgggattt
      961 ggaaggtttc accatcacgc gactgttcct tgtggccata ggcatactct ttcgcggatt
     1021 ccaaaatccc ctggatgctg gagcgagccc agtggatatg ccttggtgtg ctactcccct
     1081 tctgtggagc gttgccggca atcagtcttt cacggatctg taacagcgaa acaccctggt
     1141 tgtaccaatc aaagattttc ctgacccagt ctgcttcttc ctctacaaca tggatcttgt
     1201 cacttatgcg gatatagcca tagcgatctt gcccggtatt tgctttcccc gctttcagtc
     1261 gcgctttgac acccattccc agccgttctt tcataccgtc aagttccatc tgggcagccc
     1321 aagcacggac aggtgcgatc ctggaatcaa aattttcttt tgccagcagg atttcgattt
     1381 tatagtcctg aacggtttcc aagaccgtta acatggaacg caatccacga tacaacctat
     1441 cttcgcgcca cgcgagaatg acatcgaatt catctctggc ggcgtctttg agcattgcca
     1501 gtagaccggg gcgatctgag cggctacccg aaggttcgac cagtttgctg cccacacggt
     1561 acttttctac atcgcggtag atatgagcta cctgcaatcc tttttcttct gccaggcgac
     1621 gacaatctgc ttcctgctca actgggctgg aatttgcccc ttgtgtttca gacgatgtgc
     1681 ggatgtaaat tactgctctt cgagccattt gaataccttc ggaagaaaat atggaactac
     1741 cagttcaact ataccgaaaa aataaaatag gtggtgtcga agtgtcgcta aggaattcaa
     1801 tgtactaccg caatgaatcc ctgcataccg ggtagtatgg cgatccctac caaactctct
     1861 tcaccaaaaa gttttcattg acaatgaaaa ctttttggtt tattattcct gcatgaatga
     1921 aaacatatgg aaatccagat ggagcaagga tcaaatcaaa tccatgttgt tggagcaatt
     1981 tgattccttt tggagccgcg atttgggaat tgtacgaacg caactcgcgg aagtggagcg
     2041 ggcgagtggg gttccccatg ctgtaatcat ctccgggtta cgtcgggcag ggaaatcgac
     2101 actcttgtta caattagctc atcggttggg gcgagagcaa ttctactatc tcaattttga
     2161 ggatgatcgc tttttaggct ttcttgcaga agatgccaac gacctatacc aggcgctgct
     2221 cgaagttttt ggcgaacgca agattttcat tattgatgaa atccagaata ttgatggctg
     2281 ggaacatttt gtccgccgct ttatggacat ggggttcaaa ttttatatca cgggatccaa
     2341 cgcctcccta ctaagccggg agctgggaac ccgcctaacg ggtcggtacg tcccgattga
     2401 gttgtttcca ttttccttta aggagttcat gctgtttcgc ggagagtccc tgcctaacct
     2461 ggagcggatg acaaccgtgg accatgcccg cttgcaaaat tcccttcaat cctatttaaa
     2521 attcggcggt attccagatg cgctcaagta tccagaactc cccctcttac gtacactcta
     2581 tgatgatgtt ttgtatcgag acattgcaac acgttatcgg cttgaggcgg tgaccgctat
     2641 caaagaactc ggattctttc tgatgagcaa tccagccaat ctgatatcgt ttaataaact
     2701 caaagaccag tttcgagtcg gcagtgtaaa tacgatcaag aattacattg ggtacatgga
     2761 gaacagttgg ctgctcttta cgctgaacgt ctatgactac tcggtaaaac gccagcagat
     2821 cgcccccaag aagatctatg gaatcgatac cggcttgatc aacaccattg ggtttggttt
     2881 ttcaccaaac acaggcaaat tgctggaaaa cctggttttt ctggcgctca ggcggaaagc
     2941 aaaggagatc cattacctct cttccccagg cgggtatgag gtggatttct atttgcctga
     3001 acaacgccag ctcattcagg tttccgggaa cctggctcaa tctacaacgc gggaccggga
     3061 ggtacgcgct ctaaaggatg ccattgggac tgtaaaagta caaagcgcta ttattctgtc
     3121 ggactccaac gaggattcat tcgagatcaa tggcatcccg atacatgtac aatctactgc
     3181 tgaatggcta cttgcagaat agatgagcct gtctaacttc cccaatttca tcccgtgcaa
     3241 tactcatcat tgcaatcctt ctcgcaatga tgagtattga tttgctactt cgcgtatcat
     3301 gtttatgaag tctagtgaaa ataacctcac aagcaacaaa tgactttgat gtcgaacctt
     3361 gagattacaa caccaccaaa atctgtccgg cggggtacag tacaaatctg gcatacaggt
     3421 ataaatttcg tttacgaacg taggcggtgg atatgagaac gaagcgtcct ccaaaaatgg
     3481 aaggcgtttt tttgttttca ggcgagattg ggtatgtctt ataatggatt tatgcccacg
     3541 ccgatattag ccacgaaact cttcatccct ccgcttcgga ccaacatcgt gccacgtccg
     3601 catctgaccg agcggatgaa agaggggaag tttcataagc taacccttat ctctgcgcct
     3661 gctggttttg gcaagacaac gctggtcagc gaatggatta cgggttgtgg gctacctgtt
     3721 gcctggcttt cgttggacga gggtgacaat gatcacaaac gctttctggc atatttgatc
     3781 gccgcgttgc gcaccattac gaaaaatatc ggagagaggg cctcagactt attggatgcc
     3841 gctccattcc cagacattga atccgttctc acttctcttc ttaacgaaat caccgccacc
     3901 tcagatcatt tcattctcgt ccttgacgat tatcacctga tcgaatccaa acaggtggat
     3961 gaagcc
//
LOCUS       DAOQFT010000195         3943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12692_length_3943_cov_6.396091, whole genome shotgun sequence.
ACCESSION   DAOQFT010000195 DAOQFT010000000
VERSION     DAOQFT010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12692_length_3943_cov_6.396091"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..797)
                     /locus_tag="PLR93_06155"
     CDS             complement(42..797)
                     /locus_tag="PLR93_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26583.1"
                     /translation="MQNFTGNIGRTVTNRIGGAVAAIGAAIFMALCGALMAFVLAPQQ
                     AIKAANIEKMPQMDAAYVSAAAPGTDILITGYLNGSAPNPQLPKFIAYEAEQWDVSEN
                     KNTDGTPQAPSGSWEHVETIVPDLTLDMSGTPVNILSANNATLSGSNLHEQLVTKSSA
                     FTADYKGQKLGEGSLRYTGFFSGDLVTVLGKKSSGDGVVPEELFAGDRVAFEQYQKDS
                     AKALLFMGFAAMICSPFVGIGGVIAALLGRGRR"
     gene            950..3058
                     /locus_tag="PLR93_06160"
     CDS             950..3058
                     /locus_tag="PLR93_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH26584.1"
                     /translation="MADWIGQTIGKVRVEKYLARGGMAEVYLGTHLTLDRPVAVKVMH
                     SFVESEPELINRFQREAKAVAALRHPNIVQVYDFDTHDGHPYLVMEYLKGPSLASLLR
                     ELHKNGARLSLSQIAQLLKSLTAGIDYAHSQGIVHRDIKPANIILNNKSGDYTIDQLP
                     RDTECIITDFGLVRLTNSTTQTASGVVSGTPAYMSPEQAQGSTVDSRSDTYSLGIVLY
                     EMLAGRLPFDGDSTLGVILKHISEPPPPIEDISPEIQAVVNKALEKLPENRYQTARDL
                     LMDFYNAVGMHAEAETLYSLRLRTPPPTATLSNKPAPKPNRFLWIGAGILACLCLGVI
                     ATGAVGFSIYSLFPKATTTPQILTDAATPDHMPHGNTAEPGSTPQPISFGEFFGVLRF
                     QGNMDQVTVTASLPDPAEGTQYEAWLIDDGGELLISLGILEKNNSGQYNLTFTDPESR
                     NLLDGFNRMEITLETNPDASPNPSDTILYSSGIPAGALTHVRHLLVSFPSNPNQTPMT
                     GGLMGTSSLINEAGTALEKAFEKGDTTAIRLQAETLINLIVGKQSNEYKDWDKSGKIS
                     DPSDGFGLLLNGGQTGYIEGTITHAELSASAGDATANIKLHSEHVVISAKNVEGWATQ
                     LRDVAKRILESDQVSESDIRLAVSLAEQIYQGVDIDGNESVDPVEGEGGALTAYQHAE
                     YMADMQVLEGANLFPPPGNP"
     gene            3178..>3943
                     /locus_tag="PLR93_06165"
     CDS             3178..>3943
                     /locus_tag="PLR93_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH26585.1"
                     /translation="MTIVTAETFVQAPLKFAYRAFTNATTIREWLCDVATVAPHSRGR
                     MYLWWAGDFYSSGHYLELEENKRVKFRWYSSIDPAPTEVTVTFTEKDGGVNVRMDHEV
                     PSDEAWKKMAEGFRNNWQDSLRNLKSVLETGIDLRIAERPMLGIVPGDFSPEQAKALN
                     VPVLEGMRLDATVEGMGAKRAGIERDDVLVSMAGKPITNDFTSLQLAIAGKKGGDVIE
                     VEFYRGTKKKKVSMELTKRPMADVPFNSVELAKKARA"
BASE COUNT         1035 a         1051 c          937 g          920 t
ORIGIN      
        1 caaaacgggc ggactgtaca gtccgcccgt aaatatttaa cctaccgtct ccctcgccct
       61 aacaaagcgg cgataacccc gccaattccc acgaacggcg agcaaatcat cgccgcaaag
      121 cccatgaaga gcaaggcttt agcagagtct ttttggtatt gctcaaatgc cacccggtca
      181 ccagcgaaaa gttcttctgg caccactcca tctccagaag atttcttgcc gagcacggtc
      241 accaaatcac cgctgaaaaa gcctgtataa cgaagggaac cttctcctaa cttctgtcct
      301 ttgtaatcgg cagtgaaagc gctggatttt gtcaccagct gctcatgaag gtttgagccg
      361 cttaacgtcg cattatttgc cgaaaggatg ttaaccggcg ttccgctcat atccagggtt
      421 aagtctggaa cgattgtctc cacatgttcc caagaaccag aaggcgcttg gggtgtgcca
      481 tcggtattct tgttctcact cacatcccat tgttctgctt cataagcaat aaacttcggc
      541 aattgcgggt tcggtgcaga cccattcaaa taacccgtga tcaaaatatc cgtgccgggg
      601 gcagcagcac tcacataggc ggcatccatt tgcggcatct tctcaatatt tgcggctttg
      661 atcgcctgtt gcggtgccag cacaaatgcc atcaatgcgc cacacagtgc cataaagatc
      721 gcagcaccaa tggcagccac cgccccacca attcgattgg taactgtcct acctatattg
      781 ccggtaaaat tttgcatact cattctcctc atttggtttg atgcggcatt ataaaattta
      841 ttccgcatcg gcatcatcca ctggaagtac taatcaggct ccatttcaat accatgttcg
      901 aagaagtttt cagacacaat cgctctaatt tccgctgtat aattcgtcca tggcagattg
      961 gatcggacaa acgattggca aagtccgtgt agaaaagtat ctggcgcgcg ggggaatggc
     1021 agaggtctac ttgggcacgc acctcactct cgaccgcccc gtagcggtca aagtgatgca
     1081 tagcttcgtg gaaagtgaac ccgaactaat caaccgcttc caacgtgaag ccaaagctgt
     1141 ggcggcacta cgtcatccca atatcgttca agtctacgac ttcgacaccc acgatgggca
     1201 tccctatctt gtgatggaat atctcaaagg tccatcgctg gcaagtctct tgcgcgaact
     1261 tcacaagaac ggcgcacgct tatccctctc tcaaatcgca caattactca agtcgctgac
     1321 ggcgggtatt gactacgctc attcgcaagg catcgttcac cgtgatatca aacccgccaa
     1381 cattatcctc aacaacaaaa gcggcgacta caccatcgac caacttccgc gtgataccga
     1441 atgcatcatc acagactttg gtttggtaag gctcacaaac tccacaactc aaaccgcgtc
     1501 aggggttgtc agtggcacgc cggcctatat gtcaccagag caggcacaag gctccacggt
     1561 agatagtcgc tccgacacct attctcttgg catcgtttta tatgaaatgt tggcaggacg
     1621 tttacctttc gatggtgact ctacattggg cgtaattctc aagcacatct ctgaaccgcc
     1681 gccacccatc gaagacatct cacctgaaat tcaagccgtt gtcaacaaag cacttgaaaa
     1741 acttcctgaa aaccgatatc aaaccgcgcg tgaccttttg atggattttt acaacgcagt
     1801 gggcatgcat gcagaagccg agactttata ttccctgcgc ctccgcaccc cgccgccaac
     1861 tgccactctt agcaataagc ctgctcctaa accaaaccgc ttcctttgga tcggtgccgg
     1921 gattctggca tgtttatgtt taggggttat cgccacaggt gcagtcggat tttccattta
     1981 ttcattgttc cccaaagcga ccactacacc acaaattctc accgatgccg ccacgccaga
     2041 ccacatgcct catggcaaca cagcagaacc cggctccacg cctcagccca tctcctttgg
     2101 tgaatttttt ggtgtattac gcttccaagg caacatggac caagtcacag tcaccgcctc
     2161 cctgccagac ccggcagaag gcacgcaata tgaagcctgg ctgatcgacg atggcggcga
     2221 attactcatc agccttggaa tcttggaaaa gaataattcc ggtcaataca atctgacgtt
     2281 tactgaccct gaaagccgca atttattaga cggtttcaat cgcatggaaa tcacgctcga
     2341 aaccaatcca gatgccagcc ccaacccatc cgacacgatc ttgtattcct ctggcatccc
     2401 cgccggtgcg ttgactcacg ttcggcattt gttggtcagt ttccccagca accccaacca
     2461 gacccccatg acgggcgggc tgatgggaac cagcagtctg atcaatgagg ctgggacagc
     2521 gctggagaaa gcctttgaaa aaggcgatac gactgcaatt cgcttacaag ccgagacgtt
     2581 gatcaacctt attgtgggca agcaaagcaa cgaatacaaa gactgggaca agagcggaaa
     2641 gatcagtgac cctagcgatg gctttgggct tttgttgaac ggtggtcaga ccggttatat
     2701 tgaagggacg atcacccatg ccgaactttc tgcttcggcg ggcgatgcca ctgcaaatat
     2761 caaacttcac agtgagcatg tcgtgatcag cgcgaaaaat gttgagggat gggcaaccca
     2821 gcttcgagat gtcgcgaagc ggattctcga gtcagaccag gtttctgagt ccgatatccg
     2881 cttggcagtt tcacttgcag aacagattta tcagggtgtg gatattgatg gcaacgaaag
     2941 tgtggacccg gttgaagggg aaggcggggc attgacggca tatcaacatg cagaatatat
     3001 ggcagatatg caggtccttg aaggcgcaaa tcttttccca ccaccaggga acccgtagag
     3061 taaacgtcgt gcctttcgca cgacgttttt atttcaatag aaaacccatg ccagagtagg
     3121 ttgaatcttg ttggcaatcg ttatataaag aataaatact tactcgagga gagtttcatg
     3181 actatagtaa ctgctgagac ctttgtacaa gcccctttga aatttgccta ccgagccttt
     3241 accaatgcca caaccattcg cgaatggttg tgtgatgttg ccaccgtcgc tccgcactca
     3301 cgcggacgga tgtatctgtg gtgggctggc gatttctatt ccagcgggca ttatcttgaa
     3361 ctagaggaaa acaaacgggt gaagttccgc tggtattcaa gcatcgaccc cgctcccact
     3421 gaggtgaccg tgacgttcac tgagaaagat gggggcgtga acgtccgcat ggaccatgaa
     3481 gtaccttctg atgaagcatg gaagaaaatg gcggaaggtt ttcggaacaa ttggcaagat
     3541 agcttgcgaa atttgaagtc tgtgcttgaa acgggcattg acttgcgcat tgcagaacgc
     3601 ccgatgttgg gaattgtgcc aggcgacttt agcccagaac aagcaaaagc cctaaacgtc
     3661 cctgtgcttg aagggatgcg gcttgatgcc accgtggaag gaatgggcgc aaaacgagca
     3721 gggattgagc gagatgatgt gcttgtgagc atggcaggca agcccatcac caatgatttc
     3781 accagcctgc aacttgccat tgccgggaaa aaaggcggag atgtaatcga ggttgagttt
     3841 tatcgcggca caaaaaagaa aaaggtttcc atggaactga ccaaacgccc catggcagat
     3901 gtgccgttca actcagttga gttggcaaag aaagcccgtg cgc
//
LOCUS       DAOQFT010000196         3936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12734_length_3936_cov_8.312806, whole genome shotgun sequence.
ACCESSION   DAOQFT010000196 DAOQFT010000000
VERSION     DAOQFT010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12734_length_3936_cov_8.312806"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PLR93_06170"
     CDS             <1..1625
                     /locus_tag="PLR93_06170"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNH26586.1"
                     /translation="QPILPQRLPIMKRYAFSISYYLLLSALLAACAAPPSTPTPQPFG
                     QFILPTLDPSKATVTPFQPAGQTNTSVPTYTASPLPTSTATITPPPPTVTFTSAPAAT
                     VAVSTTQAPPPPVTTSSRPNYILYSTLDFKNRTIATDETIRYYNTTGTTLSDLVLSVQ
                     PNTYTNAFTLNAIAQDGTALTSYTLSGQRLSVNLNQPLASGSATTITLSFNIKIPQKA
                     SGEVFGYDFNQINLVDWYPFIVPYEGGWVLHDAMPWGEHLVYDAADIELNIKTDSGVT
                     LAVGASPEANGEWTRYRMYGARQLALSASNEFIVNETTWGNVTIRSYYFNGYAKGGDD
                     MLIYASEALQTYSTEFAPYPHQSLAIVQSDMDDGQEYDGLVFLATDFYSQYTGSAKSN
                     LTTIGVHEIAHQWWYSLVGSDHAFEPWLDEAMATYSERIFYENNYPANISWWWQFRVN
                     YFKPTGYVDATIYDYGSFRAYTNAVYFQGALFLDDLRELMGYGNYSKFIKTYAMRYAN
                     GHATTADFFALAREIVNVNYDNLIAKYFSRSY"
     gene            1767..2465
                     /locus_tag="PLR93_06175"
     CDS             1767..2465
                     /locus_tag="PLR93_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH26587.1"
                     /translation="MNRPYTFINVAMTADGKIDTFERKGAAISSKQDKQRVDELRAMA
                     DAILVGGKTLLEEQPKLTVKSEALREERIRQGRSPNPIKVGVATVADIQKDSDFLKAG
                     DTRVVIFTTSQTSISQIENLRALGVNVFVDDAPRVDLHKMMITLKKLGVDHLMVEGGG
                     TMNFELMRLGLVDEVLIYVAPMIFGGANSPTLADGLGVTRDHALNMHLTGVEPWDDGG
                     VILKYKFFDQRREP"
     gene            2462..3220
                     /gene="ribA"
                     /locus_tag="PLR93_06180"
     CDS             2462..3220
                     /gene="ribA"
                     /locus_tag="PLR93_06180"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNH26588.1"
                     /translation="MTTLMHEKIQVLLEEDCCHECDGYGEDHVCVHIESMAELPTRFG
                     DFHIVAFSNNRDGKEHIAIVKGDVIGEEDVPVRLHSECLTGDAIGSLRCDCRDQLEAA
                     LKKIGEMERGMVLYLRQEGRGIGLTNKVRAYALQDTGLDTVEANLALGFRDDERDYAV
                     AAHMIHSLKVKSIKLMTNNPKKIAQLEQHGVKINERMPHILPMNEHNIFYLKTKAEKS
                     GHMIDFHGKEHLPEQSDRAIVEGMSEDQISALYE"
     gene            3220..3888
                     /locus_tag="PLR93_06185"
     CDS             3220..3888
                     /locus_tag="PLR93_06185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH26589.1"
                     /translation="MNRNIIITGATGALGSLTAKTFASMGHNLALLDKDQAKLDALVR
                     DLNLPTERLFAQTVDLLNEQSLQTSAEAVLSKFGSVHALFHLIGGWVGGKTFTDTSAE
                     DLEFMINQHIRTTFNLFKVFEGPLAKNGWGRVIAVSASTVPNPPGKSGVYTATKSAQE
                     NMVLSLAAELREAHVTANIIHVRAIDVDNKGTGTTPAEIVSAMQYLFSDAADKVNGTR
                     LPLY"
BASE COUNT         1034 a         1101 c          904 g          897 t
ORIGIN      
        1 atcaaccaat acttccgcag cgtctaccaa taatgaaacg ttacgcattc agcatttcat
       61 actacctttt actttctgcc ttgcttgccg cttgtgctgc gccgccatca acacccacgc
      121 cgcagccctt tggtcaattc atcttgccaa cacttgaccc cagcaaagcc acagtcacac
      181 ccttccaacc cgccgggcag acgaacacat ccgtaccgac ctacacagca tcaccactcc
      241 caacgtcaac agcgaccatc acaccaccgc ctcccacagt gacgttcact tcggctcctg
      301 ccgcaacggt agctgtttca accacccaag cgccacctcc gccggtaacc acatcctccc
      361 gccccaacta catcttatat tccacattag atttcaagaa tcgaaccatt gccaccgacg
      421 aaaccattcg ctactacaac accaccggaa caactctctc agacttggtt ctctccgttc
      481 agccaaatac gtacaccaac gctttcactc tcaacgccat tgcgcaagat ggcacagcgc
      541 tcacctccta caccctcagc ggacaacgcc tgagcgtaaa tttaaatcaa cccttggcat
      601 cgggttctgc cacaaccatc accctgagct tcaatatcaa gatcccgcaa aaagcctcgg
      661 gcgaagtctt cgggtacgac ttcaaccaga tcaaccttgt ggattggtat cccttcatcg
      721 tgccatacga aggcggctgg gtcttacatg atgctatgcc atggggcgag catctcgtct
      781 atgatgccgc ggatattgag ttgaacatca aaactgactc cggcgtgacc ctcgcggtgg
      841 gcgcttcgcc cgaagccaac ggcgaatgga ctcgttaccg catgtacggc gcgcgccaat
      901 tggctttatc tgccagcaat gaattcattg tcaatgaaac cacttggggc aatgtcacca
      961 tccgctccta ttatttcaat ggctatgcca aaggcggcga tgacatgctc atctatgcca
     1021 gcgaagcgtt gcaaacctat tccacagaat tcgcacccta tccgcatcaa tctctcgcca
     1081 ttgtgcaatc agatatggac gatggacagg aatacgatgg cttggtcttc ctcgctaccg
     1141 atttctacag ccaatataca ggctctgcca aaagcaacct gaccaccatt ggcgtgcatg
     1201 agatcgccca tcagtggtgg tacagcttgg taggcagtga ccatgccttc gagccgtggc
     1261 tggatgaagc catggcaacc tatagtgagc gcatcttcta cgaaaataac tacccagcca
     1321 acatcagttg gtggtggcag ttccgggtca actacttcaa accgactggc tatgtagatg
     1381 cgaccattta cgattatggc tctttccgtg cttataccaa cgccgtgtac tttcagggcg
     1441 cactcttcct tgatgatttg cgtgaattaa tgggctacgg caattactca aagttcatca
     1501 aaacgtatgc tatgcgctat gccaatggac acgccaccac agcagacttt ttcgccttgg
     1561 cgcgtgagat cgtcaacgtc aattacgata atttgatcgc gaaatatttt tcaaggtcat
     1621 attaacccta actttggcaa gccctattct ctaactttcg agggttggat aaacctgccc
     1681 atcaatccta taatcaagcc atcctttatg gatggcttga tgttttgtaa gaagagctgg
     1741 gtacgacctg tcagtgaaca aatcttatga acagacctta tacctttatc aacgtcgcga
     1801 tgacggcaga tggcaagatc gacacctttg agcgcaaagg ggctgctata tcgtctaaac
     1861 aagataaaca acgtgtagac gaattgcgcg ccatggcaga tgcgattctc gtcggcggga
     1921 aaacgctttt ggaagaacag cccaagctca cagttaagag tgaagcgctg cgtgaggaga
     1981 gaataaggca agggcgttcg cccaacccca taaaggtcgg cgtggctacc gtcgcagaca
     2041 tccaaaaaga ctcagacttt cttaaggcag gcgacacgcg ggtcgtaata tttacaacat
     2101 ctcaaacatc tatatcgcag attgagaatt tgcgcgcgct gggcgtgaat gtgtttgtag
     2161 atgatgcccc ccgtgttgac ctgcacaaga tgatgatcac gctgaaaaaa ttaggcgtgg
     2221 atcatttgat ggtagaaggc ggtgggacga tgaacttcga acttatgcgg cttggtcttg
     2281 ttgacgaagt gctgatctat gtagccccca tgatctttgg cggcgcgaat tctcctacac
     2341 ttgccgatgg tctcggtgtg actcgtgacc acgccttgaa catgcacctg accggtgtgg
     2401 aaccctggga cgatggcggt gtgattttga aatacaagtt ttttgaccaa cggagagaac
     2461 catgacaacc ctaatgcatg aaaaaattca agttttacta gaagaagatt gttgccacga
     2521 atgtgatggc tacggcgaag atcacgtttg tgtacatatt gaatcgatgg cagaactgcc
     2581 cacccgcttt ggcgattttc acattgtggc attttcaaac aaccgcgatg gcaaggaaca
     2641 tatcgccatt gtcaaaggcg atgtaatcgg cgaagaagat gtgcccgtgc gtttgcactc
     2701 agaatgcctg accggcgatg cgattggctc gctgcgctgt gactgccgcg accagctcga
     2761 agcggctctt aagaagatcg gcgaaatgga acgcgggatg gttttgtatc tgcgtcagga
     2821 agggcgcggc attgggctga ccaacaaagt gcgcgcttat gctttgcaag ataccggtct
     2881 cgataccgtt gaagccaatt tggcactcgg tttccgtgac gatgaacgcg attacgctgt
     2941 tgcagctcac atgatccact ccctcaaggt caagtccatc aagctaatga ccaacaaccc
     3001 caagaagatc gcccagctcg aacagcatgg tgtaaaaatc aacgaacgca tgccacacat
     3061 tctgccgatg aacgagcaca acatcttcta cctcaaaacc aaagcagaga aatctggtca
     3121 catgatcgac ttccacggca aagaacacct gcccgaacaa agcgaccgcg ccattgtaga
     3181 aggcatgagc gaagaccaga tcagcgcgtt gtatgaataa tgaatagaaa cattatcatc
     3241 actggtgcaa ccggcgctct tggaagcctt accgccaaga cctttgcaag catgggtcac
     3301 aacctggcat tgctcgataa agaccaagcc aaattggatg ccctcgtccg cgacttgaac
     3361 ctgcccaccg agcgactctt cgcccaaacc gtggacttgc tcaatgagca atcgcttcaa
     3421 acctctgccg aagctgtact ttccaaattt ggcagtgtcc acgccttgtt ccacctcatt
     3481 ggcggatggg tcggcggcaa gaccttcact gatacctctg ccgaagatct agagttcatg
     3541 atcaaccagc acattcgcac caccttcaac ttgttcaaag tgtttgaagg tccgctcgcg
     3601 aaaaatggct ggggacgtgt gatcgccgta tccgcttcta ctgtgcccaa cccgcccggg
     3661 aaatctggcg tgtacacggc aaccaaatca gcgcaagaga atatggtgct ctctctcgcc
     3721 gccgaattga gagaagcgca tgtgacggca aatatcatcc acgtacgcgc catcgacgtg
     3781 gacaacaaag gcaccggcac cacgcccgcc gagatcgtct ctgccatgca atatctcttc
     3841 tccgatgcag ccgataaggt caacggaacg agattgccgt tgtattagga agtaacaaca
     3901 aaaaagtgac cgccgaacgg cggtcacttt ttataa
//
LOCUS       DAOQFT010000197         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12740_length_3935_cov_6.720876, whole genome shotgun sequence.
ACCESSION   DAOQFT010000197 DAOQFT010000000
VERSION     DAOQFT010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12740_length_3935_cov_6.720876"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..864)
                     /locus_tag="PLR93_06190"
     CDS             complement(106..864)
                     /locus_tag="PLR93_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HNH26590.1"
                     /translation="MSFTSHPEVDQVLSTLLENVQGILGHYFVGMYLYGSLATGDFNP
                     KSSDIDFCVVIQDTLPDEFIAHLEMMHKKIWASPFKWAAKLEGAYLPKSLIRRHTPNG
                     PACPVVNEGKFYLGTQGSDWVIQRHVLREKGVVLAGPDPITLIDYVSPEEIQNAVRGV
                     LEEWWFPMLTDSAWLVNHGSEYHAYAVISMCRALHAIEHGTIVSKPVAVEWASLQYAD
                     HARLIKKSLDAQDGTSAGFAEDALGFIDMVKKLV"
     gene            complement(867..2492)
                     /locus_tag="PLR93_06195"
     CDS             complement(867..2492)
                     /locus_tag="PLR93_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNH26591.1"
                     /translation="MLSIHNLNKHFGIQPVLKNINFNISAGERIGLIGANGSGKTTLM
                     RILAGFEHPDSGDVASTRPNLRLGYLAQGMDFSPEQTLHSALGLDSDSQTDPALEVES
                     LALALAQNPNDAALQAQYDLALHKLSGESIQPSAVLEPLGLSHFPADTPIAHLSGGQK
                     TRLMLAKVLLDDPQLLLLDEPTNHLDIDMLEWLEDWLNGFQGAALIVSHDRTFLDNAV
                     TKVLELDSSTHTIKTYDGNYSDYLEQKLLEFDKQMQAYQDQQDEIAQLKRAARQIRSL
                     TVMKKGGKSDSGDKFAKGFFGNRATKNVAGRAKNLEARIDHLLTDEKVDKPQGSWKLK
                     LDFGAPEHQSKDVLVTDNLSIGYAPETPLLTNINLFIRAGQRVVLTGANGSGKTTFIR
                     TIVGKLHPLTGSFRLGGATKLGYMAQEQELLNPALNSVQTIQSVSAINETETRNFLHY
                     FLFKGDAALRPAGDLSFGERARLQLALLVAQGCTFLILDEPINHLDIPSRERFEEALE
                     NFNGTILAVVHDRSFIERFATDVWQAKDGKILK"
     gene            2712..3557
                     /locus_tag="PLR93_06200"
     CDS             2712..3557
                     /locus_tag="PLR93_06200"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNH26592.1"
                     /translation="MTKKVLAVLAHPDDESFGLGGTLALYAQRGYETYYVCATRGEVG
                     TVSPEFLKGFKDTAEMRTDELMRAAKELGLKEVIFLDYRDSGMPGMEANQHPNAQINH
                     PVEEVAGKVVKHIRAIKPDVVITFDPIGGYKHPDHIHIHKATVLAFANADDENFHPEA
                     GEGFKPSALYFQVFPRWFLRVMTNLMPLIGKDPTKWGRNGDINLKELAEVDFPVHVRI
                     NTRSVAEIKTRASAQHASQGGTQMRRGLMGFVTRLFGEREDFMQAYPPVEGEFKRKSD
                     LFEGI"
     gene            3666..>3935
                     /locus_tag="PLR93_06205"
     CDS             3666..>3935
                     /locus_tag="PLR93_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26593.1"
                     /translation="MTQSEPIKPRFQFSRISEVLLQPQHTFALLVSENVGWRMPMLVL
                     TVSTTLVVIVSGFLKARAASMGEVTLPPDWEWWTPDMQNNYMQAQQ"
BASE COUNT         1007 a          920 c          984 g         1024 t
ORIGIN      
        1 atagccttcc gatgtaggct gcatgccaat ggtcccgccg gtgtagatga tgtatacgcg
       61 ttttttcatg aaatattccc aaggttaagt taattgattt ttacattaaa ccagcttctt
      121 gaccatatca ataaaaccca gtgcatcctc ggcaaagcct gcgcttgttc catcttgtgc
      181 atccagagat tttttgatca agcgagcatg atctgcgtat tgaagcgatg cccattccac
      241 cgcaacgggt ttcgaaacaa tcgttccatg ctcaatggcg tgtaatgctc tgcacatgga
      301 aatgacggca tatgcatggt attcacttcc atggttcact aaccatgctg aatcggtgag
      361 catgggaaac caccattctt ccaaaacacc tctcacagcg ttctgaatct cttcaggcga
      421 tacgtaatcg atcagagtga ttgggtctgg acctgcaagc acaacccctt tctcgcgaag
      481 tacatgccgt tgaatgaccc agtcactgcc ttgcgtaccg agataaaact tgccctcgtt
      541 gacaacaggg caggcaggtc cgttcggggt gtggcgacgg ataagacttt tggggagata
      601 cgcaccctcc aatttagccg cccatttgaa tggacttgcc caaatctttt tgtgcatcat
      661 ttctaaatga gcaataaatt catcaggcaa agtatcttgg atgacgacac aaaaatcaat
      721 atcactgctt ttggggttga aatctccagt tgccagtgaa ccatagagat acatgccaac
      781 gaaataatgc cctaaaatac cctgcacatt ttcgagcagg gtactgagga cctggtctac
      841 ctctggatgc gatgtaaaag acatcctcat ttcaaaatct tcccgtcttt tgcctgccat
      901 acatccgtag caaagcgctc gataaaactg cgatcatgga cgacagcgag gatggttccg
      961 ttgaaatttt ccaaggcttc ttcaaaacgt tcacgagaag gaatatcaag atggttgata
     1021 ggttcgtcca agattaggaa ggtacatccc tgtgcgacga gtaatgccag ttgtaaccgg
     1081 gcacgctcac caaaagaaag gtcaccggct gggcggagcg cggcatctcc tttgaagaga
     1141 aagtaatgca ggaagttgcg cgtctcggtc tcgttgatag ctgagacact ttgtatggtt
     1201 tgcaccgagt tcaatgccgg gttgagcagt tcctgttcct gtgccatata gccaagtttg
     1261 gtcgcgccac ccagccgaaa cgaacctgtc aacggatgga gtttgccaac gatcgtacga
     1321 atgaaggttg tcttccctga gccgtttgca cccgtcaaca ccacacgctg accagcgcgg
     1381 ataaataagt tgatgtttgt cagcaacggg gtttcaggtg cgtagccaat ggagaggttg
     1441 tcagtcacca acacatcttt tgactgatgc tccggtgcgc caaaatccaa tttcagtttc
     1501 catgagcctt gcggcttgtc aactttttca tcggttaaca aatggtcgat gcgggcttcg
     1561 aggttcttgg ctcgcccagc tacattttta gtagcgcggt tgccgaaaaa gccttttgca
     1621 aatttgtcgc cactatccga tttgccgcct ttcttcatca ccgtcaaact gcggatctgt
     1681 cttgccgcgc gtttcagttg cgcgatctcg tcctgctggt cttgataggc ttgcatctgc
     1741 ttgtcgaact caagcagttt ttgttcaaga taatcactgt aattgccgtc atatgttttg
     1801 atggtatgag tcgaagaatc caattcgagc accttggtta cagcgttatc taaaaatgtg
     1861 cggtcatgcg agacgatcaa cgccgcacct tggaatccgt tcagccaatc ttcaagccat
     1921 tcgagcatgt cgatatcgag gtgattggtg ggttcgtcca gcagcagaag ttgcggatca
     1981 tccaacaaaa ctttggcgag catgagacgg gtcttttgcc cgccagagag atgggcgatg
     2041 ggcgtatcgg cagggaaatg ggacaaaccc aacggctcca acaccgccga tggctggatg
     2101 ctctctcctg aaagtttgtg caacgctaaa tcatattgtg cttgaagcgc ggcatcattc
     2161 gggttctgag ctaatgccaa agcaagcgac tccacttcca gcgcggggtc ggtttgggaa
     2221 tctgaatcca gcccaagcgc ggagtgaagc gtttgttcgg gtgagaagtc catgccttgg
     2281 gcgagatagc cgaggcgtag attcgggcgc gtcgaagcga catccccaga atctggatgt
     2341 tcgaacccgg caaggatgcg catgagcgtg gtcttgccgc tgccatttgc gccgatgagc
     2401 ccaatgcgtt cgcctgcgct gatattgaag ttgatgtttt tgagaacggg ctggataccg
     2461 aaatgtttgt tgaggttatg gatactaagc atatgaacga actcctgtag ttaaaaagaa
     2521 aaccgagggc aagcctcggc aatattaaac gccaataggg aaatgccctg aaaaaaatgg
     2581 tttaactact tgctgtccgt cattcgctgg gtatcctttc ttgagaaaaa ttttatcaca
     2641 ttccgtaaag caggggtaaa cttgtggata gtttgaacgt caaaaacgca tcattgatca
     2701 aagaggttaa catgacgaag aaggttttag cagtactggc gcaccccgac gacgagtcgt
     2761 ttggcttggg cgggacattg gcgctttatg cacaacgcgg gtatgaaaca tattatgttt
     2821 gtgccacacg cggcgaagtt ggcactgttt ctccagagtt cttaaaggga tttaaagata
     2881 ccgccgaaat gcgcaccgat gaattgatgc gtgccgccaa agaacttggt ttgaaagaag
     2941 tcatctttct tgattatcgt gattcgggta tgcccggcat ggaagctaac caacacccga
     3001 atgcccagat caatcatccg gtagaggaag tggcaggcaa ggtggtgaag catatccgcg
     3061 ccattaagcc agatgttgtg attacgttcg acccgattgg cgggtataag cacccggatc
     3121 atattcatat tcacaaagct acggtcctcg cttttgccaa tgccgatgat gaaaatttcc
     3181 accctgaagc tggcgaaggt ttcaagccaa gtgctttgta tttccaggtc tttccacgct
     3241 ggttcttacg tgtgatgacc aaccttatgc ccctgatcgg taaggacccc accaagtggg
     3301 gtcgcaatgg cgacatcaat ttgaaagaac ttgctgaagt agatttccct gtgcatgtgc
     3361 gcatcaatac ccgttctgtg gctgagatca agactcgcgc cagcgctcaa catgcatctc
     3421 aaggcggcac tcaaatgcgc cgcggtttga tgggttttgt gacacgtttg ttcggcgagc
     3481 gagaagattt tatgcaagca tatccgccag tggaaggcga attcaaacga aaaagtgatt
     3541 tattcgaagg aatatagaaa ataaatgaag cggctggaac aaatccagcc gctttttcgt
     3601 taatcgctat taagcatgaa aatttggtgc atgttagaat ttttacgagg aaaaacggaa
     3661 tatatatgac ccaatctgaa cccattaagc cacgttttca gttttctcgt atttctgaag
     3721 ttttgttaca gcctcaacac acctttgcct tgttagtcag cgaaaatgtg ggttggagaa
     3781 tgcctatgct ggtgctgacg gtttcaacca cactggttgt cattgttagc ggatttctga
     3841 aagcccgcgc tgcttccatg ggagaagtga cgcttccgcc ggattgggaa tggtggacgc
     3901 cggatatgca gaataattac atgcaggcgc aacaa
//
LOCUS       DAOQFT010000198         3929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12774_length_3929_cov_5.667011, whole genome shotgun sequence.
ACCESSION   DAOQFT010000198 DAOQFT010000000
VERSION     DAOQFT010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12774_length_3929_cov_5.667011"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /locus_tag="PLR93_06210"
     CDS             <1..2377
                     /locus_tag="PLR93_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26594.1"
                     /translation="PYAATQTTFIVTLPEPLQAGDTLAISVVDEVTGIRFNETKYPMT
                     ARDNLTYTASLPVPVNSVIKYRYVRLGGSGAVEDTTFGTAIRYRLYSAASSAEVQDTV
                     ADWSDRTYSRPVGTILGQVYNADTGSPIPNVLVTAGGIQYITDSLGRFEISGLPVGTH
                     QLVAYSLDGLYVPFQQGAIVAESNPTIVDLRIKPTHLVNVTFFVTVPEDTVPGAPVRI
                     AGNILQLGNTFADLQGGVSTVTDRMPVMSLQPDGRYSVTLGLPAGTYIQYKYTLGDGF
                     WNAEHKSDGNWIVREFLVPSQDITIQDTVSSWLASNQSGPILFEVSVPSVTLPSDIVY
                     MQFKSAGWMEPIPMWPLGNNRWAYKLYGPLNFLGSFSYRYCRNGQCNSADDNQTIGET
                     TAGRTASTSLLGQDIQDSVGSWKWFENPESFALVGSTINPRAAGFVAGVEYQSYFRPN
                     FSSYGPQAFANTKAIGANFAVLSPSWTISNTSPLRFSAQPGNDPFWIDTAIMVSQARA
                     LGLNIALFPTPHFPPSTDSNISASDQFWKNAPKDAAWWQTFFTRYRAFLVNYADLTAQ
                     SGAQVIILGGDWITPALPGGLLPDGTSSNVPADAEAQWRSILQDVRSHFKGQILWALP
                     YEVSSFTTPVSFLRDVDGIYLLWSTPIATSSTATKTDYANETGRLLDNEVAPLASLLV
                     KPVILAVSYPSAAGAPTGCISNGSGACLDFDALSRPNVDLSSVSLNLQTQADIYEAML
                     NAVNARPWISGFVSRGYYLPVVLQDKSTSIHGKPAADVLWYWYPRLLGTVQ"
     gene            2490..>3929
                     /locus_tag="PLR93_06215"
     CDS             2490..>3929
                     /locus_tag="PLR93_06215"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNH26595.1"
                     /translation="MKKIGRFLGRMLIGILIIAVVVTSGGLFYFKSYLPKTVAPKSFP
                     QIDGEIKVDGLDGEVNIYRDAMGIPHIYATTAHDIFFAQGYVHAQDRFWQMDAWRHIG
                     SGQLSEMFGEGQVETDAFLRTLGWRKTAEAEWEQLDSDSRAILQSYTDGVNAYLKDHS
                     GTALSLEYAVLGLLSPDYVIEPWEPVNSLTWGKAMAWDLRGNMGEEIERAELLKTLTP
                     EQVALLYPEYPQDHPVIVNKTGEGGQSASTSQPSSFDYSALNLDSLSHNASLLDPVLG
                     PLGDGIGSNSWAVSGALTDTGKPYLANDPHLGIQMPSIWYQVGLHCVEKTDSCPFEVA
                     GFSFAGVPGVVIGHNDKLAWGFTNVGPDVMDLYIEKVNPENPNQYEVNGKWVDFETYE
                     ETINIVGGEPVTITVKKTRHGPVISDTYGPTKNVGDPEDTKFIPFKDRVGGVELPEQY
                     VIALAWTALTPSTPFEAIWGFNKAQNWEDF"
BASE COUNT         1007 a          973 c          904 g         1045 t
ORIGIN      
        1 cccatacgct gctacacaaa ctactttcat tgtcacatta ccagagccgc ttcaagctgg
       61 cgatacgctt gccatctctg ttgtagatga agtgactggg attcgtttta acgaaaccaa
      121 atatcccatg accgcgcgcg acaacctgac ctacacggcg tcattgccag tgccggtcaa
      181 ttcggtgatc aaataccgtt acgtccgttt aggtggttca ggtgctgtcg aagatacgac
      241 atttggaacg gctattcggt atcggttgta tagtgctgct agttctgcgg aagtgcaaga
      301 tacagttgca gattggagcg atagaactta ttctcgccca gttggaacaa ttcttggtca
      361 agtttataac gcagataccg gctcgcccat cccaaatgtt ttagtcactg caggcggcat
      421 tcagtacatc accgactctt taggacgctt tgaaatttca ggcttacctg tagggacgca
      481 tcaattggta gcatacagcc tcgatggatt gtatgttcct tttcaacaag gtgccatcgt
      541 tgccgaaagc aatcccacca ttgtggattt gcgcatcaag cccacacatc ttgtcaatgt
      601 aacctttttc gttactgtcc ctgaagatac tgttccaggc gcacctgtac gcattgctgg
      661 aaatattctg caattaggca ataccttcgc ggatttacaa ggcggcgtga gcacggtaac
      721 agaccgtatg cctgtcatga gtctgcaacc ggatgggcgc tattctgtca cactgggctt
      781 gccagcaggg acttatattc aatacaaata caccttgggc gatggctttt ggaatgcaga
      841 acataaatca gatggtaatt ggatcgtgcg tgagtttttg gttcccagcc aagatataac
      901 tattcaagac actgtatctt catggttagc atctaatcag tctggtccta tattatttga
      961 agtaagtgtt ccttctgtca cgctacctag cgacattgtg tatatgcaat tcaaatctgc
     1021 tgggtggatg gaacccatcc ccatgtggcc gcttggaaac aatcgctggg catataaatt
     1081 atatggtccg ctcaatttcc tcggctcttt ctcttatcgc tactgccgca acggtcaatg
     1141 caatagcgca gatgataatc aaaccatagg tgaaaccaca gccggcagaa cagcaagcac
     1201 cagcttgctt ggacaggata ttcaggatag tgttggttcg tggaaatggt ttgaaaaccc
     1261 agaaagtttt gcgctggttg gtagcacaat caacccgcga gcagcaggct ttgtagctgg
     1321 cgtagaatac caatcctatt tccgcccaaa cttttcttcg tatggtcccc aagcatttgc
     1381 taatacaaaa gccatcggtg ccaacttcgc tgtactctcc ccgagttgga caatttcgaa
     1441 tacatcccct ttgcgctttt ctgctcagcc cggtaatgac ccgttctgga tcgatacagc
     1501 catcatggtg tcgcaagcgc gcgctttagg cttaaatatt gcgctcttcc caacccctca
     1561 tttcccgcca tccacagaca gcaatatctc tgccagcgac caattctgga agaacgctcc
     1621 taaagatgca gcttggtggc aaactttctt cacacggtat cgtgccttcc tagtcaacta
     1681 tgcagatctc actgcccaaa gcggtgctca ggttatcatc cttggtggag attggatcac
     1741 gcctgctctc cctggtgggc ttctgcccga tggcacatct tcaaacgtcc cggcagatgc
     1801 tgaggcgcaa tggcgctcca ttctacaaga tgtacgctcg catttcaaag gtcagatact
     1861 atgggcatta ccatatgaag tttccagctt cactacccct gtaagtttct tgcgtgatgt
     1921 agatggaatt tacttgttat ggtctacccc aatcgcaaca agttccacag caacaaaaac
     1981 agattacgcg aatgaaaccg gtcgcttgct cgataatgag gtcgctccgc tggcatcttt
     2041 attggtcaag ccggtcatcc ttgccgtctc atatccatcc gcagcaggcg cccctacagg
     2101 gtgtatatcc aacgggtctg gagcctgttt agactttgat gccctctctc gtcccaatgt
     2161 agatttatcg tctgttagtc ttaacttgca aacccaggca gatatttacg aagccatgct
     2221 caatgcggtc aacgcccgac catggatttc tgggtttgtc agccgtggat attatctccc
     2281 tgttgttttg caagataaat ccacatccat tcatggcaag cccgcggcag atgtgttgtg
     2341 gtattggtac cccaggttgt taggaaccgt tcaataattt attttcttca tttatgaatc
     2401 agacgcagag tgagctacga aactctgcgt ctggtgtatt aagtactgcc acagagccca
     2461 tgactctgtg gaaaaccatt ggaggaacta tgaaaaaaat tggcaggttt ttagggagga
     2521 tgttaatcgg aattttgatc atcgctgtgg tggtcacctc gggcgggctt ttttatttca
     2581 aaagctattt accgaaaaca gtcgcgccaa aatcttttcc acaaattgac ggagagataa
     2641 aagttgacgg cttggacgga gaagtgaata tttatcgcga tgcgatgggc attccgcaca
     2701 tctatgcgac cacagcacac gatattttct ttgctcaggg ctatgttcat gctcaagacc
     2761 gtttctggca aatggacgcc tggcgtcata ttggttcagg gcaactctct gaaatgttcg
     2821 gcgaaggtca agtagaaaca gatgccttct tacgaacgtt gggatggcgc aaaacagcag
     2881 aagctgaatg ggaacaactt gactctgatt cacgtgccat cttgcaatca tataccgatg
     2941 gtgtgaacgc ttatctcaaa gatcatagcg gaaccgccct cagcttggaa tatgccgtgt
     3001 tggggttgct cagccccgat tacgtcattg aaccgtggga acctgtcaat tcgttaacat
     3061 ggggtaaagc gatggcatgg gatctccgtg gcaacatggg tgaagaaatc gaacgtgcag
     3121 aattgttaaa aactctcaca ccagaacagg ttgcattgct ttacccagaa tatcctcaag
     3181 accatcctgt catcgtgaac aaaactggtg aaggtggaca atctgcatca actagtcaac
     3241 cttcaagctt tgactattct gccctcaatc ttgattcact ctcacacaac gcctcattac
     3301 tggacccggt acttggtccg cttggcgatg gaattgggtc aaactcttgg gctgtctccg
     3361 gcgcattgac cgacaccggc aaaccttatc tcgccaatga cccgcatctt ggcattcaaa
     3421 tgccgtctat ttggtatcag gtcggtttgc attgcgtcga aaaaacagat agctgtccgt
     3481 tcgaagtagc tggcttctca tttgctgggg ttcccggtgt agtaatcggt cataacgaca
     3541 aactcgcatg gggctttacg aatgtaggac cagatgtgat ggatctgtac atcgaaaagg
     3601 tcaacccaga aaaccccaat caatatgagg tcaacggcaa atgggttgat tttgaaacat
     3661 acgaagaaac catcaacatt gtcggtggtg aaccggtaac tatcacggta aaaaagaccc
     3721 gccacggacc ggtcatctca gacacttacg gtccaactaa aaatgttggc gacccagaag
     3781 acacgaagtt cattccattc aaagaccgtg taggcggagt agaactgccc gaacaatacg
     3841 tgatcgccct cgcatggacg gctttaaccc catccacacc atttgaagcc atttggggtt
     3901 ttaacaaggc acaaaactgg gaagacttc
//
LOCUS       DAOQFT010000199         3926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12784_length_3926_cov_7.518729, whole genome shotgun sequence.
ACCESSION   DAOQFT010000199 DAOQFT010000000
VERSION     DAOQFT010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12784_length_3926_cov_7.518729"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLR93_06220"
     CDS             complement(<1..769)
                     /locus_tag="PLR93_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH26596.1"
                     /translation="MASILEVQNLEKNYGDFKAVKGISFDIKEGEIFSLLGPNGAGKT
                     TTISMLSTLYTPTAGDATIAGHSITKAPMAVRNAIGVVPQDLALYEDLNARENLTFWG
                     QMYGLGGSALKNRVEEVLNQIGLVDKAKDRVKTYSGGMKRRVNIGVGLLHKPKLLFMD
                     EPTVGIDPQSRRAILDTVKDLNKQGMTVLYTTHYMEEAEELSDRVGIIDHGELIAIGT
                     QKELTKQVGETETLILHISENEDPEALVTAFKGIKGVL"
     gene            complement(786..1340)
                     /locus_tag="PLR93_06225"
     CDS             complement(786..1340)
                     /locus_tag="PLR93_06225"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNH26597.1"
                     /translation="MLKYALLGFLSYSPLTGYELKQRMDSSTNNFWNANLSQIYTSLK
                     SLEEDGWVKSKIQAQDDKPDKRIYTLTDEGLSSLKAWLEEPITDPEQRKSTFLLKLFF
                     SAKVDKETSLAQLRVSKNLHQREKERYKSEAQTSIKDIVATYPSMKKEAIFWEATRRF
                     GELYEEMYVHWLSETIELIENKMK"
     gene            complement(1489..2556)
                     /locus_tag="PLR93_06230"
     CDS             complement(1489..2556)
                     /locus_tag="PLR93_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNH26598.1"
                     /translation="MKKIRARDLGIPFEGTPGAFNAITDVPGITVGYSTIIEGTDART
                     GVTIIHPRSMDNPTPVFAGVHSFNGNGEMTGAAWVDEGGILEGPIGLTNTHSVGVVHH
                     ALIKWQVEHHKLTQNWLTPVVAETADAWLNNMNGFFVQEHHVLEAINSAKSGEIDEGN
                     VGGGTGMLCYEFKGGTGTSSRKLPEKLGGWTVGAIAQCNFGRRYQLTIAGVPIGHHLT
                     DDAPATNGENPFKQDDGSLIVIIATDAPLLPHQLKRVAKRVSLGMSRTGAIGGNGSGD
                     IFLAFSTANSEAAKGLGRTQLEALPNGQLDPIFAATAYATEEAIVNALVAAEDMTGHE
                     ISAKALPHEDVKELLKKHGRL"
     gene            2678..3169
                     /locus_tag="PLR93_06235"
     CDS             2678..3169
                     /locus_tag="PLR93_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH26599.1"
                     /translation="MANDTVKDGLVVSMEYKLTVDGEVLDSSDEAGPLQFLAGYGNII
                     PGLENEMMGMKIGDSKNVTVQPADGYGEFEDDAFVDVPRTEFPADMEIEEGMELHVTD
                     EDGNHQAAYVAEFDDKTVKLDFNHPLAGAVLDFYVKVVALREPTDEELDHGHVHEGDG
                     HHH"
     gene            complement(3203..>3926)
                     /locus_tag="PLR93_06240"
     CDS             complement(3203..>3926)
                     /locus_tag="PLR93_06240"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNH26600.1"
                     /translation="GDLASKRYDQGIPNTGTSSGTIPTCCTDSTVTADSKPMTDTISG
                     FFLTQVINYGAPLFALTLFLGAVGLPVGASLMVIAVGAFSQQGYFEWTTMALLGLLGA
                     ALGDALSFGMGHYGKGWVEKRFGTSPTWKNAQSYFDKRAWVAIFLTRFLVTALAIPTN
                     LIAGGSGYKFQRFMLYDVLGEMTWIILYGGLGYAFGSQWEVVYDFIGNFGGFVLGAVI
                     LGAGIWMARNQFNKPKQEQLAV"
BASE COUNT          987 a          921 c          916 g         1102 t
ORIGIN      
        1 cgagcacgcc cttaatgcct ttgaatgccg tcactaaagc ttcggggtct tcattttcgc
       61 taatgtgcaa gatcaacgtt tcggtttcgc ccacttgttt ggtgagttct ttttgggtac
      121 caatcgcaat caactcacca tggtcaataa tgcccacacg atcggaaagt tcttcggctt
      181 cttccatgta atgcgtggtg tagaggacgg tcatgccttg cttgttcaag tcttttaccg
      241 tgtcaagaat ggcacggcga gattgtggat cgataccaac cgttggctcg tccatgaaga
      301 gaagctttgg tttgtggagc aagcccaccc caatgttgac gcggcgcttc atcccgcccg
      361 aataggtttt gacacgatct ttggctttat ccaccagccc gatctgattc agtacttctt
      421 ccacgcggtt cttgagagct gagccaccca atccatacat ctgaccccag aaggtgagat
      481 tttcacgagc gtttaagtct tcgtacagag caaggtcttg aggcacgaca ccaatggcat
      541 tccgcaccgc catgggagct ttggtaatgg aatgacccgc aatggtcgca tcgccagcgg
      601 taggggtgta gagcgtggaa agcattgaga tggtggtggt cttcccggcg ccgttcggtc
      661 cgagtaagct gaagatttcg ccttctttga tatcaaacga aatccctttt acagctttaa
      721 agtcaccgta gtttttttcg aggttttgta cttccagtat agatgccatg attgttcctt
      781 tgtgttcact tcattttgtt ttcaataagt tcgattgttt cagataacca atgtacgtac
      841 atctcttcgt aaagttctcc aaatcttcgc gtggcttccc agaaaatggc ttcttttttc
      901 atacttgggt aggttgcgac gatgtcttta atacttgttt gtgcttcaga tttgtatcgt
      961 tctttttcgc gctggtgcag gtttttggat accctaagct gggcaaggga tgtttcttta
     1021 tccacctttg ccgagaagaa aagttttagc aagaatgtgc tttttctctg ctcagggtcg
     1081 gtaataggct cctcgagcca cgcctttaga ctgctaagcc cttcatcggt caacgtgtag
     1141 atgcgcttat ccggtttatc gtcttgagcc tgaatctttg atttaaccca gccatcttcc
     1201 tctagagact tcaacgaagt gtagatctga ctaaggttcg cattccagaa attgtttgta
     1261 gaactatcca tacgctgttt tagctcgtac cccgttaaag gggaatagct gagaaacccg
     1321 agtaaggcat atttgagcat aaggcatcct ttatatataa atttttatat ataactgttt
     1381 atatattata ggttttgttt tgtcaagggc tcttaaacta caaaccctag tcataaacga
     1441 ctagggttta tagaattttc gtatagtcag ttaagcggat ttactgattt ataacctgcc
     1501 gtgtttcttc aagagttctt tcacatcttc atgtggcaaa gccttggcgc tgatctcatg
     1561 ccctgtcata tcttcagctg caacaagcgc gttgacgatc gcttcttctg ttgcataagc
     1621 tgtggcggca aagatcgggt caagttgacc gtttggcaat gcctcaagct gggtccgtcc
     1681 cagtcccttt gccgcttcag aattcgctgt ggaaaatgca aggaaaatat caccagagcc
     1741 atttccgcca attgcgcccg tacgactcat tccaagagag acccgctttg cgacacgttt
     1801 cagttgatgt ggtagaagtg gggcgtcggt tgcaatgatg acgatcaatg aaccatcatc
     1861 ctgcttaaac ggattttcac cattggtggc aggggcgtca tctgttaggt gatgcccaat
     1921 aggtacgcct gcaatcgtaa gttgataacg tctgccaaag ttacattgcg ctatcgcgcc
     1981 gactgtccaa ccgccaagtt tttcgggcag ttttctggat gatgttcccg ttccgccctt
     2041 gaattcgtag catagcatac ccgtcccacc gcctacgttg ccttcatcga tctcacccga
     2101 tttggcagag ttgatcgcct caagaacatg gtgttcttgt acgaaaaaac cattcatgtt
     2161 attgagccag gcatctgcgg tttcagctac aacgggggtt aaccaatttt gagttaattt
     2221 atggtgttca acctgccact tgatcaaggc atgatgaaca acgccaacgc tatgcgtgtt
     2281 tgttaatcca ataggacctt ccaaaatacc gccctcatct acccaggcag ccccggtcat
     2341 ctcaccattg ccattaaaag aatggactcc ggcgaatacc ggggttgggt tatccatact
     2401 gcgtggatga atgatcgtca caccggtgcg tgcatctgtc ccttcgataa tggtggagta
     2461 tccaaccgta ataccaggca cgtctgtgat tgcgttgaat gccccaggtg ttccctcaaa
     2521 agggatgccc agatcgcggg cacgtatttt tttcatacat tctccttggc aaagcattga
     2581 aaagacgcca acattatagc cgaaggggtt ctgtggatgc cttgcagtct atttattgct
     2641 ggtacaatgg gaacattaaa ttcgaaagga tacaagtatg gcaaacgata cagtaaaaga
     2701 cggtttggtg gtttccatgg aatacaaact gaccgtagac ggcgaagttt tggactcttc
     2761 tgacgaagca ggtcctttgc agtttctggc ggggtacggc aatatcattc ccggtttgga
     2821 aaatgaaatg atgggaatga agatcggcga cagcaaaaat gtcacagttc aaccagccga
     2881 tggatatggc gagttcgaag acgatgcttt cgttgatgtt ccacgcactg aattccctgc
     2941 tgatatggaa atagaagaag gtatggaatt gcacgttacc gatgaagatg gtaatcatca
     3001 ggctgcgtat gtggctgaat tcgatgacaa gaccgtcaag ctcgatttca atcatcccct
     3061 tgctggcgct gtgttggatt tctatgtcaa agttgtagct ctccgtgagc caactgacga
     3121 agaactcgat cacggtcatg tccacgaggg cgatggacat catcactaat aaaaaaacat
     3181 cctctgtata gaggatgttt ttttatacag ccagttgttc ttgtttgggt ttattgaatt
     3241 gatttcttgc catccaaatg ccagcaccta agatcacggc acctaataca aacccaccaa
     3301 agtttccaat aaaatcatag accacttccc attgagaccc aaatgcatat cccaacccgc
     3361 catacagaat gatccaggtc atttcgccga gaacatcata tagcataaaa cgttgaaact
     3421 tatatccgct gccaccagca atcaggtttg tgggaattgc caacgccgtt accaaaaagc
     3481 gagtgagaaa aatggctacc caggcacgtt tatcaaaata ggattgagcg ttcttccaag
     3541 tgggggaggt gccaaagcgc ttttcaaccc agccttttcc ataatgaccc attccaaagc
     3601 ttagagcatc cccaagtgca gccccgagca gacccagaag cgccatcgtg gtccattcga
     3661 aatatccttg ttgagagaaa gcgccaacag caataaccat cagagaggca cctacgggta
     3721 gcccaactgc tccaagaaaa agcgtcagcg caaaaagagg cgccccgtag ttaataactt
     3781 gtgtaaggaa aaatccagag atcgtatcgg tcatgggttt tgagtcggcg gtgacggtgg
     3841 agtcggtgca acaggtggga atggttccgg aggacgttcc tgtgtttgga atgccttgat
     3901 cgtatctttt acttgcgaga tcgcca
//
LOCUS       DAOQFT010000200         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12821_length_3918_cov_7.202433, whole genome shotgun sequence.
ACCESSION   DAOQFT010000200 DAOQFT010000000
VERSION     DAOQFT010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12821_length_3918_cov_7.202433"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLR93_06245"
     CDS             <1..311
                     /locus_tag="PLR93_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH26601.1"
                     /translation="TPAASPTPTLDPFAPPTVDVTTIHIDGYVQIIEVGTDGLRIRSA
                     PGLNTETVFLGGESEVFLVKDGPQDADGYTWWYLVASYDDTRAGWAASEFLTVVPPPQ
                     "
     gene            343..678
                     /locus_tag="PLR93_06250"
     CDS             343..678
                     /locus_tag="PLR93_06250"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNH26602.1"
                     /translation="MTEKPTGITANKSKREFIVNWDSGHVSAYPFGLLRAACPCASCR
                     GGHENMKSEPDADVFTKQMEDNSSTRIANVVAVGSYALTIVWEDGHDYGIYNWHYLRK
                     LCPCSECRK"
     gene            complement(741..2630)
                     /locus_tag="PLR93_06255"
     CDS             complement(741..2630)
                     /locus_tag="PLR93_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH26603.1"
                     /translation="MTGELKGQFNHPNSEQNNDTIFQDAVNALRSGDKPRAKELLTLL
                     LKTDQNNPTYWIWLSASVDANKERIYCLQTALKLDPENSIAKRGLILLGALSPDETIQ
                     PFPMNRPRAWEEKLLLAHEKPKERGVRVVMRSPLVRLGGLAIIGIGLCAVIYFGVILP
                     RWGTIQLAPTNTPGPSPTFTATPTIFGATAIPTQVFSGPTPLSAFLNATYTPTALYVN
                     TPRSAVSVDQFRIAKQAYDNKDWDVFIENMLLIEPLEPDSVDIPYYIGEAYRFKGDTT
                     SAARYYRDAMNMNPAFGPAYLGLARVSLMSNPDFNPEKLFNQAIEYDPNLGEAYLERA
                     RWLIRRGDYADALPDLELADKIMPGSPEVYLAYANSYLGDGDKEKALEYAQKAYEADV
                     LNLPTYKLLGDLYIEKEDYANAAEALRLYTTYESQDATGFGKLGEAYYYLGEYQNAVL
                     AIDAGEQLNRNGMKKYMIYRGLSHVELGNFNEAEGDLNIAVDDEPESFEARLGYAKAL
                     YGVSKFGSAFLQAESMRSLADTDEEKALELYWRAKIQEQRNDIRDAIKSWNDLLKLDE
                     SVMTPEMRQEAQEHLKTLVPPTNTPKGGAPTSTPVPVTKTPQTGTTPTVGKTPTKAPS
                     ATPTP"
     gene            complement(2640..3215)
                     /gene="amrA"
                     /locus_tag="PLR93_06260"
     CDS             complement(2640..3215)
                     /gene="amrA"
                     /locus_tag="PLR93_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNH26604.1"
                     /translation="MSALLTDKERQTLLHIARVSIENAVKGNPQSPLEKDTLTPVLRE
                     NGASFVTLTINGELRGCIGALEAYQPLAEDVREHAVAAALNDPRFPALREAELSRIQI
                     EVSYLTKPQELQYSTSQDLLAKLHPHRDGVILKYGRRRATFLPQVWEKLPKPEEFLSQ
                     LCYKMGERQTLWQETKLQVYVYQVEEFHELN"
     gene            complement(3269..3760)
                     /locus_tag="PLR93_06265"
     CDS             complement(3269..3760)
                     /locus_tag="PLR93_06265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNH26605.1"
                     /translation="MTLDPEALRSAMRAWSAGVTVVTAVHEGEMHGMTVNSFTSISLT
                     PPLLIISLQQNTRTHELVMKSRAFGLTILSSEQSKVSDLFAGRMPEVEDRLATLKTET
                     LVTGSPLIVGGLAWLDCRVLQTYDAGSATLFIAEAVSARGTGSGDPLIYHNREYWKLS
                     KLK"
BASE COUNT         1010 a          956 c          932 g         1020 t
ORIGIN      
        1 acacccccgc cgccagcccc acgcctacac tcgatccttt tgctcctccc acggttgatg
       61 tcaccaccat ccacattgac ggatatgtgc aaatcatcga agtcggcaca gacgggttac
      121 gcattcgctc tgcacccgga cttaatactg aaaccgtctt ccttggtgga gaatcagaag
      181 tctttctcgt caaagacgga cctcaagacg cagatggcta cacatggtgg tatctcgttg
      241 catcgtacga cgatacccgt gcaggctggg cagcctccga atttctgaca gtcgtcccac
      301 cgccgcaata aaattattga tcgaaataat caaaggaaca acatgaccga aaaaccaact
      361 ggaattacag ccaacaaaag caaacgtgaa tttatcgtca actgggatag cgggcacgtc
      421 agtgcatatc cctttgggtt attaagagcc gcttgcccct gcgcctcctg ccgcggtggt
      481 cacgagaata tgaaatcgga gcccgatgcc gatgtattca ccaaacaaat ggaagataat
      541 tcttcaaccc gtattgcaaa cgtggtcgca gtcggctcgt acgcgctgac catcgtttgg
      601 gaagatggtc atgactatgg catctataac tggcactatc tgcggaagtt atgtccatgc
      661 tcggaatgta gaaagtaaca tccccaaaaa taaaaaacag gtaggttttc aacctgcctg
      721 tttttttctc attattcaaa tcatggtgta ggcgttgcag atggagcctt ggtgggggtc
      781 tttccaactg tcggtgtagt gccagtttgc ggggtttttg tgacaggcac cggtgttgat
      841 gtaggagcgc cgcctttggg tgtatttgtt ggcggaacaa gggtcttgag gtgctcctgt
      901 gcttcctgac gcatttccgg cgtcatcacg ctttcatcaa gtttcaacaa gtcgttccaa
      961 gatttaatcg catcacgaat gtcatttcgt tgttcttgta tcttcgctcg ccaatacagc
     1021 tcaagcgcct tttcttcatc tgtatctgcc agcgagcgca tggactcagc ctgcaagaaa
     1081 gcactgccaa atttgctaac accatacagg gctttggcgt acccaagccg ggcttcaaag
     1141 gactcgggtt catcatcgac cgcaatattg aggtcacctt cggcttcatt gaaattgcca
     1201 agttccacat gcgataaacc gcggtagatc atgtatttct tcatgccatt gcggttgagt
     1261 tgctcacccg catcgattgc aagcacagca ttctgatact cgccaagata gtaatacgcc
     1321 tcacccaact tgccaaaacc agtagcatct tgagactcat aggtcgtata caaacgcaac
     1381 gcttctgctg cattagcata gtcttctttt tcaatataaa gatcgcctaa aagcttatac
     1441 gttgggaggt tgagaacatc ggcttcgtac gctttttgag catattccaa tgccttttct
     1501 ttatcaccat cgccaaggta ggaatttgca tacgcaagat acacttctgg cgagcctggc
     1561 atgatcttgt ctgcaagttc caaatctggc agggcatcgg cataatcgcc acgccgaatg
     1621 agccaacgag cacgttcaag atacgcctcg cccaaatttg ggtcatattc aatggcttga
     1681 ttgaataatt tttcagggtt gaaatctggg ttcgacatca gacttacgcg ggcaagccct
     1741 aaatatgccg ggccaaaggc aggattcata ttcattgcat cgcggtagta acgcgctgcg
     1801 ctggtggtat ctcccttaaa acgataggct tcgccaatgt aataaggaat atctactgag
     1861 tctggctcaa gaggttcgat cagaagcata ttctcaataa agacatccca gtctttatta
     1921 tcataggctt gctttgcgat acggaactga tccactgaga cggctgaacg tggagtatta
     1981 acatacaaag cggttggcgt gtacgttgca ttcaaaaagg cagaaagcgg agtcggtccg
     2041 ctaaagacct gggtaggaat tgctgttgcc ccaaagattg ttggggttgc agtaaacgta
     2101 ggagaaggac ctggggtgtt ggtgggagca agttgaatcg tgccccaacg cggaaggata
     2161 accccaaaat aaattacagc acacaaacca atgccaataa ttgccaaccc acccagccgt
     2221 accaacgggc ttcgcatgac gacacgcacg ccacgctctt ttggcttttc atgagcaagc
     2281 agtaacttct cttcccacgc ccgcggacgg ttcataggga agggttgaat ggtctcatct
     2341 ggcgaaagag ctccgagtag aatcaagccg cgtttggcaa ttgaattttc ggggtccagt
     2401 ttaagagccg tttgcaaaca atagatgcgt tctttattgg catccactga ggcgcttagc
     2461 caaatccaat acgttgggtt gttttggtcc gtttttagga ggagggttag caactctttg
     2521 gcgcggggtt tgtctccgct gcgcagagca ttcacggcat cctgaaaaat ggtgtcgttg
     2581 ttttgttcgc tattaggatg gttaaattgc cctttgagtt cgccagtcat gcgtccattt
     2641 taattcaatt catggaattc ttcgacctga tatacataaa cttgcaattt tgtctcctgc
     2701 caaagagttt ggcgctcccc catcttgtag caaagttgac ttagaaattc ttccggcttg
     2761 gggagcttct cccatacttg tggcaagaag gtggcgcggc ggcgcccgta tttcaaaatg
     2821 accccatccc gatgtgggtg tagtttggca agtaaatctt ggctagtgga gtactgcaac
     2881 tcctgcggtt ttgtgagata agaaacttcg atttgaatac ggctcaattc cgcttcacgc
     2941 agggctggaa aacgcgggtc gtttaacgcc gctgcaacgg catgctcacg gacatcttct
     3001 gcaaggggtt gatacgcctc caaagcgcca atacaaccgc gtagttctcc attgatcgtt
     3061 agggtcacaa aagatgctcc attttcacgg agaactggtg tgagtgtatc cttttcaaga
     3121 ggtgattgag ggtttccctt tacggcattt tcaatagaca ctctggcaat gtgcaaaaga
     3181 gtttgtcttt ccttatctgt caacaaagca gacatggcat cctcacatga atacaaagaa
     3241 cgccaagcgt tttggcttgg cgttttcttt acttcagttt cgataatttc caatactcac
     3301 ggttgtgata gatcaagggg tcgccgctgc cagttccacg agccgaaact gcttcggcaa
     3361 taaataatgt agccgaaccc gcgtcataag tttggaggac gcggcaatcc aaccaggcga
     3421 gtccgcctac gatcagggga gagccggtta caagagtctc ggtcttcaac gttgctaggc
     3481 gatcttccac ttcgggcatg cgcccggcaa acaaatccga gaccttcgat tgttcagaag
     3541 aaagaatagt taaaccaaag gcgcgcgatt tcatcaccag ctcatgcgta cgagtattct
     3601 gttgcaaaga aataatgagc aagggcgggg tcaacgaaat agatgtgaat gaattaacgg
     3661 tcatgccatg catttcgcct tcgtgcacag cagtaaccac agtgacacct gccgaccacg
     3721 cacgcatggc agatcgcagg gcttcagggt ctagggtcat aacaggggaa ttctccatag
     3781 atatttctcg ttttcatcat aattgccgta aaagagcaag tcaagccaaa aaccgcatag
     3841 tttcagcaat tttgaatact ctttgcaaga cattgattca atggcaaaac aaaaacttac
     3901 cttaatcttc ctttatgc
//
LOCUS       DAOQFT010000201         3916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12827_length_3916_cov_8.203056, whole genome shotgun sequence.
ACCESSION   DAOQFT010000201 DAOQFT010000000
VERSION     DAOQFT010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12827_length_3916_cov_8.203056"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLR93_06270"
     CDS             <1..471
                     /locus_tag="PLR93_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26606.1"
                     /translation="ILLRRAVIYGLGRVREEWATDLLKKVQIEDEQWVVRNAATEVLD
                     MQAQSYAYAPMKPKAPHETPWLIEFAAKKGVGVSPGVPATDILLVALKDENPETRLAA
                     IQFLKYTPQEIVLKQLYEAMYKDDPELREATFNILWELGAAGVKLPHPSEYGLN"
     gene            473..1825
                     /gene="ssnA"
                     /locus_tag="PLR93_06275"
     CDS             473..1825
                     /gene="ssnA"
                     /locus_tag="PLR93_06275"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HNH26607.1"
                     /translation="MLITNANIITWESPNRVLSNYAILIEGGKIKEIGTTKSLTAKHP
                     KVKATDVNGQYVMPGGICAHTHFYGAFARGMAIPGPAPKDFPDILKKLWWPLDRSLDA
                     EGVRYSALVSIVDAIKHGTTTLIDHHASPNTIDGSLDVIADVVDKSGLRGVLCYEVTD
                     RDGIEKANAGIQENVRFIKRLASNPHPRLAAAFGLHASLTLSGATLQACAAAAPEGTG
                     FHVHTAEHESDEYDSLQKSGMRVIDRLLKHGILGPKTITAHGVHFDAREMEILKETET
                     WLTHQPRSNMNNGVGTAQIESMLRLGIKVCLGNDGFTNSMWDDWKVAYLLHKAEHRDP
                     RRMGGYEVQQMAIYNNAALAQNFFPEAPIGQIIPGAAADLIFVDYHPFTPLTDGNLPW
                     HIIFGFQQSMVTSTMVAGKFLMKDRELLTLNEEEIAARAREIAPRIWKKYEEEVAKVM
                     "
     gene            1849..2523
                     /gene="npdG"
                     /locus_tag="PLR93_06280"
     CDS             1849..2523
                     /gene="npdG"
                     /locus_tag="PLR93_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNH26608.1"
                     /translation="MKEDHILVSVAVLGGTGKEGKGLAYRWARAGYHVLIGSRSEEKA
                     VAAAKEVTEMLGDGVSVAGMNNLQAAKEANIVVLTVPYAAHRETLESVKDALQGKILV
                     DVTVPLVPPKVATVQMPAAGSAAQEAKQILGEGVEVCAAFQNISHEHLLGGEGVECDV
                     LVTGTSKDARANVILLVEAAGLRGWDAGPIENSVVVEGLTSVLIGINKKYGSTHAGIK
                     ITGISK"
     gene            2578..3348
                     /gene="cofE"
                     /locus_tag="PLR93_06285"
     CDS             2578..3348
                     /gene="cofE"
                     /locus_tag="PLR93_06285"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNH26609.1"
                     /translation="MSLTLNTLKNIPLIRQGNNLADILLKSLHESGIALQDNDILVLA
                     QKIVSKTEGRMVNLAKIEPSSAALELAPIIDKDARLIELILQESNEVLRIRKGVIVVE
                     HKLGFVCANAGIDHSNVMGDGKEDEEYVLLLPENPDTSARWLREEILRKTDKTVGIMI
                     IDSHGRAWRNGTVGVCIGLSGIPALVDERGWKDLFGYTLKATIVGVADELAAAASLMM
                     GQAAEGTPVVHVRGFPYPLSESGSLQELIRPKEMDMFR"
     gene            3422..3814
                     /locus_tag="PLR93_06290"
     CDS             3422..3814
                     /locus_tag="PLR93_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNH26610.1"
                     /translation="MPFPENFLDLIQPETKSFLYLATVNAKGVPQVTPVWFDTDGEHI
                     LINTNEGRLKDRNMKKNENVAMVIQDPKSPYRYLGMQGKVVSYTTEGADEHINLLSLR
                     YDEEPWKYREGQKRIIFKIKPTSFDEHN"
BASE COUNT         1074 a          963 c          952 g          927 t
ORIGIN      
        1 attctcctgc gccgtgctgt gatttatggt cttggacgtg tccgcgagga atgggcgact
       61 gacctgctta agaaagttca aattgaagac gaacaatggg ttgttcgtaa tgccgctaca
      121 gaagtgttgg atatgcaggc acaaagctat gcctatgcac ccatgaagcc taaagctccg
      181 catgaaaccc catggcttat tgagtttgcc gccaagaaag gcgtgggcgt ttcacccggt
      241 gtccctgcca cagatatttt gttggttgcc ctgaaagatg aaaaccctga aactcgctta
      301 gctgccattc aattcttaaa gtacacaccg caagagattg tgctcaaaca attgtacgaa
      361 gccatgtata aagatgaccc cgaattacgg gaagcaacct tcaacatcct ttgggaactt
      421 ggcgcagcgg gcgtcaaatt accgcaccct agcgaatatg gattgaacta atatgctgat
      481 cacaaacgca aacatcatca catgggaatc tcccaatcgc gtcctttcaa attacgccat
      541 tcttatcgag ggcggaaaga tcaaggaaat cggcacaacc aaatcgctta cagccaaaca
      601 tcccaaagta aaagccactg acgtaaacgg tcagtatgtg atgccgggcg gcatttgcgc
      661 acacactcac ttttatggtg cttttgcgcg cggcatggca attccgggtc cagcgccaaa
      721 ggattttcct gacatcctca aaaaattatg gtggccactt gaccgttctc ttgatgcaga
      781 gggcgtgcga tactctgcct tggtttctat cgtggatgca atcaagcatg gcaccactac
      841 tctcattgat caccatgcat ctcccaacac tattgacggc tcgctagacg tcattgctga
      901 tgtggtagat aaaagcggct tacgtggtgt gctttgctac gaagtaacag accgtgatgg
      961 aatcgaaaaa gccaatgcag ggattcaaga gaatgtgcgc ttcatcaaac gcctcgcctc
     1021 aaatccacac ccgcggttgg cagctgcttt tgggctgcat gccagcctga cactttcggg
     1081 cgcaaccttg caagcctgtg ccgccgcagc ccccgaaggg acaggcttcc acgtccatac
     1141 agccgagcat gagtccgatg aatacgatag cttgcaaaaa tctggcatgc gtgtgattga
     1201 ccgcttgcta aaacatggca tcttaggtcc aaagaccatc acggcacatg gtgtccactt
     1261 tgatgcacgt gaaatggaaa tcctcaaaga aaccgaaacc tggcttactc atcagccccg
     1321 ttctaatatg aataatggtg tgggcacagc tcaaatcgaa tccatgctcc ggcttggcat
     1381 aaaggtctgt ttgggcaatg atggcttcac caattccatg tgggacgatt ggaaagtggc
     1441 atatctcctg cataaagccg aacatcgcga tccacgccgc atgggcgggt atgaagtgca
     1501 acagatggcg atctacaaca atgctgccct tgctcagaac tttttccccg aagcacctat
     1561 tgggcaaatc atccccggtg ctgccgcaga cttgatcttt gtggattatc accccttcac
     1621 cccgctaaca gatggcaacc tgccatggca catcatcttt ggttttcaac aaagtatggt
     1681 gactagcact atggtagctg gaaaattttt gatgaaagat cgtgagttgc tcactctcaa
     1741 cgaagaagaa attgcagcgc gcgcaaggga aatcgctccg cgcatttgga aaaagtacga
     1801 agaagaagtc gccaaggtca tgtagagaac gtccctttag gagaactcat gaaagaagat
     1861 cacattttag tttcagttgc tgtcttgggc ggcacaggca aagaaggcaa agggctagcc
     1921 tatcgttggg ctcgcgccgg gtatcatgta ttgatcggtt ctcgttcaga ggaaaaagcg
     1981 gttgcggcag ccaaagaagt gacagaaatg ctcggcgatg gagtctctgt cgctggaatg
     2041 aacaatcttc aggcggcgaa agaagcgaac atcgtcgtgt taaccgttcc atatgcggca
     2101 catcgcgaaa cactggaatc cgtcaaagat gcattgcaag gcaaaatcct tgttgatgtg
     2161 acggtacctc tcgtcccgcc gaaagtggct acagtgcaaa tgccggcagc gggttctgcg
     2221 gcacaggaag ccaaacaaat tctcggcgaa ggcgtggaag tttgtgcagc gttccagaat
     2281 atttcgcatg agcacttgct cggcggcgaa ggcgtggaat gcgatgtact tgttacgggt
     2341 accagtaagg atgcgcgtgc aaatgtcatc ctgttagtgg aagcggctgg tctacgtggt
     2401 tgggacgcag gaccgattga aaattcggtt gtagttgaag gtctcaccag cgttttgatc
     2461 ggtatcaata aaaagtacgg ttctacccat gccggaataa agatcaccgg aatctctaaa
     2521 taagcctccg tgcgtaatgc gtacatttga cgtgttgcgc attacaaaac gcgtatcatg
     2581 tctctcactc taaacacact caaaaatatt cccctcatcc gccaaggtaa caacctggcg
     2641 gatattcttc ttaaatcatt gcacgaatca ggcattgctc tgcaagataa tgacattctc
     2701 gtgcttgcac agaagatcgt tagcaaaacc gaaggacgca tggtcaacct tgcaaagatc
     2761 gaaccttcat ctgcggcact tgaacttgcc ccgatcattg ataaagatgc tcgcctgatc
     2821 gaattgatct tgcaagaaag caatgaagtt ttacgtattc gcaaaggggt aatcgttgtt
     2881 gaacataaac ttgggtttgt atgtgccaac gccggcattg accattccaa tgtcatgggc
     2941 gatggcaaag aagatgaaga atatgtactg cttctgccag aaaacccaga tacatctgca
     3001 agatggttgc gcgaagaaat actacgcaaa acagataaaa ccgtcggcat tatgatcatc
     3061 gactcgcatg ggcgtgcctg gcgcaatgga acggtgggtg tttgtatcgg gctcagcgga
     3121 attccagccc ttgtcgatga acgcggatgg aaagacctct tcgggtacac gctcaaagca
     3181 actattgttg gtgtagcaga tgaacttgcc gccgccgctt ccctaatgat gggtcaagcc
     3241 gcagaaggta cgccggttgt acatgtgcgc gggtttccgt acccgctcag cgaaagcggc
     3301 tcacttcaag agcttattcg ccccaaagaa atggatatgt tccgataagc tctaacagga
     3361 atcttatgcg cccttgatat aaaaggtacg caaggctcga ataaaattcc tggagaatac
     3421 aatgcccttc cctgagaact tcctcgacct cattcaacct gagaccaaat cttttcttta
     3481 tcttgccacc gtcaatgcaa agggggttcc acaagtgacc ccggtatggt tcgatacaga
     3541 tggtgaacat attcttatta acacaaacga aggtcgcctc aaagatcgca atatgaaaaa
     3601 gaacgaaaac gtggcaatgg tcattcaaga cccgaaaagc ccgtatcgtt atctcggcat
     3661 gcaaggcaaa gtggtttctt acacaactga aggcgcagat gagcatatca acttgctttc
     3721 tttgcgttac gatgaagaac catggaaata ccgcgagggg caaaagcgga ttatttttaa
     3781 gatcaagccg acttcctttg acgaacataa ttgatctgca cacctttctg cggacaaggc
     3841 gctcgattcg ccgcttcaaa ccggacccga ttcctgattc agtcatccgg gaaattctcc
     3901 acactgccac tctcgc
//
LOCUS       DAOQFT010000202         3916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12828_length_3916_cov_7.794095, whole genome shotgun sequence.
ACCESSION   DAOQFT010000202 DAOQFT010000000
VERSION     DAOQFT010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12828_length_3916_cov_7.794095"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLR93_06295"
     CDS             <1..509
                     /locus_tag="PLR93_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26611.1"
                     /translation="YLWKKGAEYAQRESTTRTTFSFNLGEQHPVYEVLDDKLPAVRKP
                     YAYYVRVPDVPAFLNQVKPALEKRLSNSVAVGYSGELRVGFYKDGFRMVFENGNITLI
                     EALKHTTDSDVDIAFPEYSFLHVLFGHRSLDELRHAFVDCYVQNPTARVLVNALFPKR
                     DSFVQAVS"
     gene            511..1764
                     /locus_tag="PLR93_06300"
     CDS             511..1764
                     /locus_tag="PLR93_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26612.1"
                     /translation="MFRIEASKVYYFIEFTSSAFFSMMFVVISLYEATVAGLPPMQLV
                     LVGTTLEVSAFLFEIPTGVVADVFSRRLSIILGYIMMGVGFLVEGLFPSFLPILLAQV
                     IWGVGYTFTSGATQAWITDEVGEEKANPLFMRAARAGLVASLIGMGATMLIGANNAAM
                     PIIVGAIGVILIGIVLIFIMPETGFKPTAMENRSTWQHMWHTFNEGVKSVKARPRLMN
                     ILGVGFFYGLYSEGFDRLWVKHLLDTFQIPVLFGGNQVAFFSLLRFSAGLLTVFVMQL
                     IEKRVDASSPLSIGRAMIIVTGGIAVGIVGFALSSLLPLTLGLYLTIYILRQVRIPLQ
                     TAWVNHKLDPQTRATVHSMTGQVDSIGQILGGGSAFIARYLSVVAAITTSGILLGPAL
                     GLVRRANSQSTHEAEVDPVGELTGD"
     gene            1891..2697
                     /locus_tag="PLR93_06305"
     CDS             1891..2697
                     /locus_tag="PLR93_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNH26613.1"
                     /translation="MALRGNLRDFTITQLLNLINLASKTGTLVIDGASEQAHISFREG
                     KLAYARIGKEDGSLALVLYKANKINANQYKAIIDRAGQMSDKELGLLLINAGYVSRDD
                     ILLNLQAYFTDMVRRMFTWVEGVFRFEAEMLPPEDRINVRLDLENIIIEGSRQLRELE
                     QLQDEIPSLDMALKFTDRPLTNVNLSVDEWKVVKFVDPKNTMRQIAKTNKLTEVEVRR
                     IVYGLLQAGLVELVRPADAPTPQAAKPLAPQEMEDKKNLVNKLIGRIRSL"
     gene            2725..3261
                     /locus_tag="PLR93_06310"
     CDS             2725..3261
                     /locus_tag="PLR93_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNH26614.1"
                     /translation="MQTVKMVVTGPFSAGKTQFIQSVSEIDVVATERKISSDEERSVK
                     SATTVAMDFGRITVDQDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETFR
                     EARSILETFRAYAPTPFVVAANKQDKEDAWDLEDMGHALRLDKNTKLLPCVATDRNSV
                     KTILLELLYSIMSEMEGK"
     gene            3396..>3916
                     /locus_tag="PLR93_06315"
     CDS             3396..>3916
                     /locus_tag="PLR93_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH26615.1"
                     /translation="MAYLGAFYRTYALDFWGFGESGTKRSTYAVKDFVSLVYQFMEQL
                     GIVRAPLVGHSMGGTVSLMTAMQHPDRIQKVVVIGSPISGSSLYFFPRVFGYKTFGWL
                     TYHNLWIYKGFYRILSPFYSKDKNWAKMMDRDVSQVSLEAFFSSIGSLRRTDLRPSLH
                     TIQAPVMGMYGDK"
BASE COUNT          921 a          866 c         1039 g         1090 t
ORIGIN      
        1 gttatttgtg gaagaaaggc gcagaatatg cccaacgcga atccacgaca cgaaccacct
       61 tcagcttcaa ccttggtgaa cagcatcccg tttatgaagt gctggacgat aaactgccgg
      121 cggtgagaaa gccctacgca tattacgtcc gcgtgccaga tgtgcccgcc tttttgaacc
      181 aagtgaagcc ggcactggag aaacggttaa gtaactctgt tgcagtaggg tatagcggag
      241 aactacgggt tgggttttat aaagatggtt tccgtatggt ctttgaaaac ggaaacatca
      301 ctttaatcga agcgctaaaa cacactacag atagtgacgt agacattgcc ttcccagaat
      361 attctttctt gcacgtttta tttggtcacc gcagcctaga tgaattgcgt catgcgtttg
      421 tggattgtta tgtacaaaac cccacagcac gtgtattagt caacgcgctt tttccgaagc
      481 gtgattcgtt cgtgcaggca gtgtcgtaat atgttccgca tagaagcctc caaagtgtat
      541 tatttcatcg agttcacatc ttcggcattc ttttcgatga tgttcgtggt gatttcttta
      601 tatgaagcca ccgtggctgg gttgcccccc atgcagctcg tacttgtcgg aacgactttg
      661 gaggtttctg cttttctatt tgaaattcca accggtgtgg tggcagatgt cttctcacgc
      721 aggctgtcca ttatcctcgg gtacattatg atgggagtag gctttttggt cgaaggtcta
      781 ttcccatctt ttcttccgat tttgcttgca caagtgattt ggggcgtggg ctataccttc
      841 accagcggcg ccacacaagc ttggattacc gacgaagtgg gggaggagaa agccaatccg
      901 ctttttatgc gggcggcacg agcgggcttg gtcgcttcgt tgatcgggat gggcgccaca
      961 atgcttatcg gtgcgaacaa tgcagctatg ccaattatcg ttggcgccat tggtgtgatt
     1021 ctgattggca ttgttttgat cttcatcatg cctgaaacgg gcttcaagcc tactgctatg
     1081 gagaatcgca gcacatggca acacatgtgg cataccttca atgaaggtgt gaagtctgtg
     1141 aaggcgcgcc cgcgtttgat gaatatttta ggcgtcggct ttttctatgg tttgtatagt
     1201 gagggcttcg accgtctttg ggtgaagcac ttgctcgata cctttcaaat cccagtcctg
     1261 tttggcggta atcaagttgc tttcttttcg ctgttgagat tttcagcagg cttgctgaca
     1321 gtcttcgtaa tgcagctcat agaaaaacgc gtagatgcga gtagtccact ttcgattgga
     1381 agggcgatga tcattgtgac gggaggcatt gccgttggta ttgtcggatt tgccctctcc
     1441 tcgcttcttc ctttgacgtt gggcttgtat ctcaccattt acattttgcg tcaggtgagg
     1501 attcctctgc aaaccgcttg ggttaatcat aagcttgacc cgcagactcg tgccaccgtc
     1561 cactcgatga cgggacaagt agattcgatt ggacaaattc tgggcggcgg gtcggcgttt
     1621 attgcgcgct atctctcggt agtggcagcc attacaactt caggcattct gcttgggcca
     1681 gcactcggct tggttcgcag agccaactca cagtccacgc atgaggcgga ggttgatcca
     1741 gtaggggagt tgacaggcga ttaaaagtcg gaatagcggg gaaatttgat aaatcctacc
     1801 catacaagag tgcaaacagg tttaattgac gctttatctg tttaccaagt ataatttcga
     1861 gcgctcccta tgaagggaat ttgaggaatt atggctctgc gcggtaattt aagagatttt
     1921 acaatcactc aacttttgaa cctcatcaat ctcgccagta agacgggaac attggtgatt
     1981 gatggtgcgt cggaacaggc acatatttcg ttccgagagg gcaagttagc ttatgcccgc
     2041 attggcaagg aagatggaag ccttgctttg gttctttata aggcaaataa gatcaatgcc
     2101 aatcagtaca aagccattat cgaccgcgcc gggcagatgt ctgataaaga gttggggttg
     2161 ttgttgatca atgcagggta cgtttctcgt gacgatattc tgctaaattt gcaggcttat
     2221 tttacagaca tggtgcgccg catgttcaca tgggtggaag gggtgttccg ctttgaggcg
     2281 gaaatgttgc cccctgaaga ccgcatcaat gtacggcttg accttgagaa tattattatc
     2341 gaaggttcac gtcaactgcg cgaattggag cagttgcagg atgaaattcc aagcctggat
     2401 atggcgctga agttcaccga ccgcccgttg accaatgtca acttgagcgt ggatgaatgg
     2461 aaggtggtca agtttgttga cccgaagaat acgatgcgcc agatcgcgaa gacgaataag
     2521 ttaacggaag ttgaagtacg ccgtattgtt tacggtttat tgcaagcggg tcttgtggaa
     2581 ttggtgcgcc ctgccgatgc gcccacgcca caggctgcaa aaccgcttgc tccgcaagaa
     2641 atggaagaca aaaagaattt ggtcaacaaa ctgatcggcc gcattcgttc gttataattt
     2701 tttcaggccg aaaggatagg aattatgcaa acggtcaaaa tggtagtcac aggtccattc
     2761 agcgctggca agacccagtt tattcagtct gtcagtgaga tcgatgtggt tgccacagaa
     2821 cggaagattt cctcagatga ggaacgctcg gtgaaatctg ccacaaccgt ggcgatggat
     2881 ttcgggcgca tcacagttga ccaggatctg gtcctgtacc tgttcggcac ccccggtcag
     2941 aaacggttcg actttatgtg ggagatcctt tctgaaggta tgttgggatt catcgtgatg
     3001 gtggatagca cacgcccaga gaccttccgt gaagcgcgtt cgattctcga aaccttccgc
     3061 gcttatgctc ctactccctt cgttgtggct gcaaataagc aggataagga agacgcttgg
     3121 gacttggaag atatggggca tgccttgcgc ctcgataaaa atacgaaact gctgccctgc
     3181 gttgcgacag accgcaactc cgttaagacc attttgcttg agctgttgta cagcattatg
     3241 agcgaaatgg aaggaaaata gtgggcgggt gatgtcttac tgtgtcttca attacgactg
     3301 atcagggaat cgttcattac gaggttttcg gtcgggggaa accggtcatc cttttgcatg
     3361 ggtatcaggg ttcatggggg ttatggcagg aaaccatggc atacttgggc gcgttttatc
     3421 gcacctatgc tcttgatttt tggggttttg gcgaatcagg taccaagcgt tccacctatg
     3481 cggtaaaaga ctttgtaagt ttggtttacc aatttatgga gcagttgggc atcgtccgtg
     3541 cgccattggt ggggcacagc atgggcggga cggtcagctt gatgaccgca atgcagcacc
     3601 cagaccggat tcaaaaagta gtggtgatcg gttcacccat ctcagggtct tctctgtatt
     3661 tctttccgcg tgtttttggt tacaagacct ttggttggct gacctatcat aacctttgga
     3721 tctataaagg tttttatcga atcctttctc ccttctattc aaaagataaa aactgggcaa
     3781 agatgatgga ccgtgacgtt tcacaggttt ccttggaagc attcttttca agcattggct
     3841 ccctacgccg tacggacttg cgcccaagct tgcataccat tcaagcccct gtgatgggga
     3901 tgtatggtga caaaga
//
LOCUS       DAOQFT010000203         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_12884_length_3907_cov_5.080478, whole genome shotgun sequence.
ACCESSION   DAOQFT010000203 DAOQFT010000000
VERSION     DAOQFT010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12884_length_3907_cov_5.080478"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLR93_06320"
     CDS             <1..652
                     /locus_tag="PLR93_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNH26616.1"
                     /translation="NELETLNDAVGSYRLANYLVDLGMYRSAVFAARQVLTIAGLDEH
                     TESMMAPAYFSHIRYGLYYSDLVIPTSQQEGFDPLFIFSVIRQESLFEGFVRSNAGAH
                     GLMQIIAPTGAQIASELGKPLNYSEEDLYRPYVSVLFGTHYLARNRTLMNGDLYATLA
                     AYNGGPGNAAAWKDLSGEDQDLFLESVRFEETRNYIRNIYEIFIVYKRLYGTPITQ"
     gene            complement(735..1043)
                     /locus_tag="PLR93_06325"
     CDS             complement(735..1043)
                     /locus_tag="PLR93_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNH26617.1"
                     /translation="MSREYVLHDGKRGSALAVRITPRASRNQIAGVLNDGTVKIQLAA
                     DPDDKNLNSDLLAFLAEVLGVPRTRVEIVAGESGRDKLISVLDMDVETVHQRIVAHVE
                     "
     gene            complement(1053..1307)
                     /locus_tag="PLR93_06330"
     CDS             complement(1053..1307)
                     /locus_tag="PLR93_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNH26618.1"
                     /translation="MNGNTIISIIIIVADFFVWVVIASSLLSFFLPPYHPVREALGKI
                     VDPFLNPIRSLMPSGGGLDFSPLILILIIELITNFLRSLL"
     gene            complement(1304..2047)
                     /locus_tag="PLR93_06335"
     CDS             complement(1304..2047)
                     /locus_tag="PLR93_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNH26619.1"
                     /translation="MSELVNSIRERYLSALDTIASAANRSGRSPEAVKLVVVTKFQPL
                     EVVQAAIEAGAVILGENYAEEGVTKIQSLQNLSAVEWHMIGHVQSRKAPLVAGHFNFM
                     HSLDSLKLAKKLDRFCGEAGRVLPVLLEFNVGGEDSKGGWEAFDETRWEELLPEVSEL
                     LGMPNLQVRGLMSMPPLGMDAEFARPYFQKLRRLQEFFTSHLPQAKWIELSMGTSSDY
                     EVAVEEGATFVRIGTAIVGPRNYQVAENL"
     gene            complement(2077..2856)
                     /gene="pgeF"
                     /locus_tag="PLR93_06340"
     CDS             complement(2077..2856)
                     /gene="pgeF"
                     /locus_tag="PLR93_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNH26620.1"
                     /translation="MPFDQSDGLRYYQFDIFSKNILNAVFTRQGGISPEPWTSLNLSI
                     SVGDDPARVAENRVHAFNALGRNPASIHDAWLIHGTDVIFADAPRSLDAPTQKADILF
                     TDNPEVSLFMRFADCVPLLFHDPKKNVIGISHAGWMGTVKGVAEVSVQAMQERYGSNP
                     QDIVVGIGPSISADHYEVGEDVAAQFREKYGKDSEQVVQVRDEKIYLDLWAANALQLQ
                     KMGVEHIQISGLCTACHLDDWYSHRAEKGKTGRFGVLLAMV"
     gene            complement(2856..>3907)
                     /locus_tag="PLR93_06345"
     CDS             complement(2856..>3907)
                     /locus_tag="PLR93_06345"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNH26621.1"
                     /translation="AGIILKPAKGLSHMLNVTLFTKKDCKLCDEVKADLKALQEQYPH
                     RLAEVDIESDAALYEKFRNVIPVIEVGPYTVQAPISRQKLQMTLGAASDRKNQLEKLD
                     DPVYKYKMEKGKKLSTGDRVSFWISKRYLLLLNLFMLLYVGLPFLAPTLMKLGAEAPA
                     QLIYRIYKPLCHQFAFRSFFLFGEQPFYPLAEANLTGYKTFEQATGIAGVDDPYNMAR
                     FEARNYIGDSTIGYKVALCERDVAIYLAILAFGIIFGLTGRRFKSVHWLVWILIGIAP
                     IGLDGFSQLFSQFNWDWLNTLLPYRESTPFLRVLTGALFGLFTAWFAYPNIEESMNET
                     RQYFLKKSAVIEAAE"
BASE COUNT         1071 a         1010 c          862 g          964 t
ORIGIN      
        1 caatgaactt gaaacactca acgatgccgt tggcagttac cgcctcgcca actacttagt
       61 cgatttaggc atgtaccgtt ctgctgtttt tgccgcccgc caagtattga ccatcgcagg
      121 cttggatgaa cataccgagt ctatgatggc acctgcctat ttcagccaca tccgctacgg
      181 gttgtattat tcagatttag tcatccctac ttctcaacaa gaagggtttg acccattatt
      241 tatatttagc gtaattcggc aagaaagttt atttgaaggc tttgtccgct caaatgcagg
      301 cgcccatgga cttatgcaaa ttatcgcccc taccggagcg caaatcgcct cagaactcgg
      361 aaagcctttg aactattccg aagaagatct atatcgcccc tacgtcagtg tcttgtttgg
      421 tacacattac ctagctcgta accgcacttt gatgaatggt gacctttatg ccaccctcgc
      481 cgcctacaat ggcggacccg gcaacgccgc cgcttggaaa gacctctctg gcgaagacca
      541 agatctcttc ttggaaagtg tacggtttga agaaacacgt aactacatcc gcaatattta
      601 cgaaatattc atagtgtata aacgattata tggcacaccc ataacccagt aagtgcgagc
      661 ctgaataaaa tagagggatg aatagactct ttcacagagt ctattcatcc ctctattatt
      721 taatcaaatt cgttttattc tacgtgcgca acaatgcgtt gatggacagt ttccacatcc
      781 atatctaaca cagagatgag tttatctctg ccgctttcac cagcgacaat ttccacacgg
      841 gtgcgaggca cgcccagcac ttcagcgaga aaggcaagca agtcactgtt cagattttta
      901 tcatcggggt cggcagccaa ctgaatcttg acagtgccgt catttaagac cccagcgatc
      961 tggttacggc tggcacgtgg ggtaatacgt acagccaagg ctgaaccgcg cttgccatca
     1021 tgaagaacat attctctgga catatcatca cctcacaata gggaacgtaa aaaattagtt
     1081 attaactcaa tgatcaagat caaaatcagt ggactaaaat caagcccgcc accgctaggc
     1141 atcaaacttc gaatcgggtt taagaacggg tcaacaattt ttcccaatgc ttcccgcaca
     1201 gggtgatagg gtggtagaaa aaacgagagc aacgaagatg caatcacaac ccatacaaag
     1261 aagtcagcaa cgatgatgat gatggatatt atagtattgc cgttcataaa ttctccgcta
     1321 cttgatagtt tcttggtcca acaatggcag tgccgatccg tacgaacgtg gcaccttctt
     1381 ccacagcaac ttcataatca gaactcgttc ccattgaaag ctctatccac tttgcttgag
     1441 gcaaatggct cgtaaagaac tcttgcaagc gccgcagctt ttggaaatac gggcgcgcaa
     1501 actctgcgtc cattccaagc ggcggcatag acatcaaacc gcggacttgc aaattaggca
     1561 taccaagcaa ctcagaaact tcgggcaata actcttccca gcgcgtttca tcaaaggctt
     1621 cccaaccgcc cttgctatct tcgccgccca cgttgaactc taataagacc ggcagcaccc
     1681 tgcccgcctc accgcaaaac cgatccagct tcttcgcaag ttttaggcta tccaatgaat
     1741 gcatgaagtt aaagtgccct gccaccagcg gcgcctttcg gctctgcacg tgacctatca
     1801 tgtgccattc taccgcagaa agattttgca aagattgaat tttcgtgaca ccctcttcag
     1861 catagttttc gcccaaaatg acagccccag cctcgatcgc cgcttgaact acctccagcg
     1921 gctgaaattt ggtgacgacg accaacttca ccgcctccgg acttcgcccc gacctgttcg
     1981 ccgctgacgc gatcgtatcc aacgccgaca gataccgctc tcgtatagaa ttgaccaatt
     2041 cgctcatatg attattttaa cccacccagg gttttctcaa accattgcca acaacacgcc
     2101 aaaacgacct gttttccctt tttcggcacg gtgtgaatac caatcgtcta aatgacaggc
     2161 agtgcataag cccgatatct gaatatgttc cacacccatc ttctgcaatt gcaaagcatt
     2221 tgccgcccac agatcaagat aaatcttctc atcccgcacc tgaacaactt gttcagagtc
     2281 tttgccatat ttctcacgga attgcgcagc aacgtcctca cctacctcat aatgatctgc
     2341 actaatagac ggaccaatgc cgaccacaat atcttgcgga ttggaaccgt atcgttcttg
     2401 catggcttgc acagagacct cagcgactcc tttcacagtt cccatccaac ctgcgtgtga
     2461 aatgccgatt acgtttttct tcgggtcgtg aaaaagcaaa gggacacaat ctgcaaagcg
     2521 catgaagagc gaaacttcag ggttgtcggt aaaaaggata tctgcttttt gagtcggagc
     2581 atcgagcgag cgcggggcat ccgcgaagat aacgtctgtg ccgtgaatca gccatgcgtc
     2641 gtggatagag gcggggtttc gtccaagcgc attaaaggca tggactcggt tctcagccac
     2701 gcgcgcgggg tcatctccaa ccgaaatgct taagttgaga gaagtccacg gttcggggct
     2761 aatgccgccc tgacgagtaa acaccgcatt gaggatattc tttgaaaaga tatcgaactg
     2821 gtaataacgc agcccgtctg attggtcaaa gggcattatt cagccgcctc gatcactgct
     2881 gactttttga ggaaatactg acgggtttcg ttcatggatt cttcgatgtt gggataagca
     2941 aaccaagccg taaagagacc aaagagcgcg ccggtaagaa cacgtaagaa gggtgtgctt
     3001 tcgcgatacg gtagaagtgt attgagccaa tcccaattga actgactgaa gagttgcgaa
     3061 aagccgtcta acccgatggg cgcaatgccg atcaaaatcc acaccagcca atgtacagac
     3121 ttgaagcggc gaccggtgag tccaaagatg atgccgaacg ccagaatagc aagatagatc
     3181 gctacatcgc gttcgcaaag cgccacttta taaccaatgg tcgaatcacc aatatagttg
     3241 cgggcttcga agcgagccat attgtatgga tcatctacac ctgcaatgcc ggtggcttgc
     3301 tcaaaggtct tatagcctgt gaggttcgcc tctgccagcg gataaaaagg ctgttcgcca
     3361 aacaagaaga aagaacgaaa agcaaattga tggcaaagag gtttatagat gcgatagatc
     3421 agttgcgcgg gtgcttcggc gcccaacttc atgagggttg gcgcaagaaa aggcagccct
     3481 acatacaaca acatgaacag attcaacaaa agcaaataac gctttgatat ccagaatgaa
     3541 acacggtctc cagtagacaa tttctttcct ttctccattt tgtatttata aacggggtcg
     3601 tccaattttt cgagctgatt tttacggtcg gaggctgcgc ccagagtcat ttgcagtttc
     3661 tgacgtgaga tgggcgcttg aacggtatac ggtccgactt cgatgacggg gataacattt
     3721 ctaaattttt cgtacaacgc cgcatctgat tcaatgtcca cttctgccag acggtgcgga
     3781 tattgttctt gcagcgcctt caagtcggct ttgacttcat cacagagctt gcaatctttt
     3841 ttcgtgaaga gtgtgacatt gagcatatgc gaaagtccct ttgcaggttt caaaataatt
     3901 cccgcag
//
LOCUS       DAOQFT010000204         3882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13017_length_3882_cov_6.025085, whole genome shotgun sequence.
ACCESSION   DAOQFT010000204 DAOQFT010000000
VERSION     DAOQFT010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13017_length_3882_cov_6.025085"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLR93_06350"
     CDS             complement(<1..587)
                     /locus_tag="PLR93_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNH26622.1"
                     /translation="MRNAKIVATIGPASDSEQALESLIKAGMNVARLNFSHGTHEQHQ
                     VRVKRIREVSERLNMPVGILQDLQGPKIRVGKLDIPLQLAVNEEVCLYATNDTPPQNG
                     CQLIPVDFRELFESVQKGDKLLLDDGRLALQVTSAEGRQVHATVKVGGALSSHKGINL
                     PGVKLRIAGFTSKDKADLAFGISQGVDAVAISFVR"
     gene            complement(797..2107)
                     /locus_tag="PLR93_06355"
     CDS             complement(797..2107)
                     /locus_tag="PLR93_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNH26623.1"
                     /translation="MNPSSIYQSRVTTAEEAIKQVKSGNRVFLTGNVSVPKVLLSALV
                     GHAPNINDVEVCQALTIGAADYVTEAMEGHLRVNSMFISANVRKAVQDGRADFTPVLL
                     SEFPLLFKRGILPVDVALIHVSRPDEHGFCSLGVEVGLTKSAAESAKIIIAEVNDQMP
                     RTLGDSFIHVSRLNYIVPVNYPIPEHKMAAEGDSDIVQDIAQHVASLIPDGATMQLGI
                     GSIPDAVLKFLMDKKDLGIHTELFSDGVIELVNAGVLTNARKSLHPGKIVAGFILGTQ
                     KLYDWVNDNPMVEMHPTEYVNDPFIIMQNERMVAVNSAIEVDLTGQVCADSIGPKLYS
                     GVGGQLDFIYGASRSKGGVPIIALPSTATMRDGTKVSKITAMLKPGAGVVTSRNHVHY
                     VVTEYGIADLYGKTIRQRAQALIKVANPIFHDELEKQAKELHYL"
     gene            complement(2104..3195)
                     /locus_tag="PLR93_06360"
     CDS             complement(2104..3195)
                     /locus_tag="PLR93_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNH26624.1"
                     /translation="MYIGIPKESRPFEYRVGLSPAGVEILSHLGHQVFVEHDAGASAG
                     FKDQDYEKAGGRIAFSAQEVFGRADFLLKVSRPIKQEMEWINDGAILAGFLHLASASQ
                     DQIDLLLEKKITALAYEQITDAQGGRAVLRPFSLICGAMVAQIAARWLQTNRGGKGIM
                     LSGVPGVPPAEVVIIGGGAVGTSAAQAMQNAGAHVTVLDKNMAALENIHDKLPGVVTM
                     MATQRNIERATAYADVVVGAVLVAGQRAPMVITREMVRAMKPRSVIIDVSIDQGGCVE
                     TSRPTMHDNPTFIEEGVVHYCVPNIASTVARTASHVFLNSAIPYITAIANTGIDAAMK
                     NDPSIEAAVNTHQGKLVNLVRLGGVEVKE"
     gene            complement(3254..>3882)
                     /locus_tag="PLR93_06365"
     CDS             complement(3254..>3882)
                     /locus_tag="PLR93_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH26625.1"
                     /translation="PIQGEAGVIMPPAGYLKKVAELCKKNNVLYISDEIQTGLGRTGK
                     LFAANHEDVRPDVVIVGKALAGGFYPVSAVLADKPILGLFTPGEHGSTFGGNPLAAAV
                     ARASLKVLREEKLADRAAELGTYFVEQLSEIPSPHVKEVRGKGLLIGVELKPEAGGAR
                     RFCEALKSKGILAKETHDNVIRFAPPLVIDKDTIDWALPAIRETLNMP"
BASE COUNT          990 a         1048 c          889 g          955 t
ORIGIN      
        1 ctgcggacga aagaaatcgc aaccgcatcc acgccttggg agataccaaa agcaaggtcc
       61 gctttgtctt ttgaagtaaa gcctgcaatg cgaagtttta caccaggcaa gttgatgcct
      121 ttgtgcgaag ataatgcccc gccaactttc acagtagcat gaacttgcct gccttctgcc
      181 gaagtgactt gcaatgcgag gcgcccgtca tccaaaagta gcttatcgcc cttctgtaca
      241 gactcgaaca gctcacggaa gtccacaggg atcaattggc agccgttctg tggcggcgta
      301 tcgttagtgg catacaagca aacctcttca tttaccgcaa gctgaagagg aatatctaat
      361 ttacccacac ggatcttggg cccctgcaaa tcttgcaaaa tgccaactgg catattaagc
      421 cgttctgata cttctcgaat tcgcttcaca cgaacttggt gctgttcatg ggtgccgtgg
      481 gagaagttta accgggcgac attcatgccc gccttgatca aggattccaa cgcctgctct
      541 gaatctgagg cgggaccgat cgttgctaca atttttgcgt tacgcatagt gctgcttcct
      601 gtcaataatg atttgttctg cgttgaaaat aaaatccatc gcaaacccgt taaaaaatac
      661 ggggtgtttg tgtctcaaaa cgccccgtat gataggtcta aaacagagga atgtcaagcc
      721 ggggcaaatt attatgggtt taacaataaa aagggcatgg agttacgcaa actccatgcc
      781 ctttttgggt actcttttaa aggtaatgca gttctttcgc ctgcttttcc agctcatcat
      841 ggaaaatcgg gttcgccacc ttgatcagtg cctgagcccg ctggcggatg gtcttgccgt
      901 acaagtccgc gatgccatac tctgtcacca cataatggac atgattacgg ctcgtgacca
      961 cgccggcacc tggttttagc atggcagtga tcttactcac cttggtgcca tctcgcatgg
     1021 ttgcggtgct gggcaaagca atgatgggca caccaccttt ggaacgagac gccccgtaaa
     1081 tgaaatctaa ctgcccaccc acaccgctgt atagcttcgg accaatgcta tcggcacaga
     1141 cttgcccagt gaggtccact tctatggcag agttgaccgc taccatgcgt tcattctgca
     1201 taatgataaa cggatcattc acatactccg tcgggtgcat ttcgaccatt gggttatcgt
     1261 tgacccaatc ataaagtttt tgggtaccca agataaaacc agccacaatc ttgcccgggt
     1321 gcagagattt gcgagcattg gtcaacactc ctgcattgac caattcgatc acgccgtctg
     1381 agaataactc ggtatgaatc ccgagatctt tcttatccat cagaaatttc aacacagcat
     1441 cagggatgga accaataccc agttgcatgg ttgcgccatc gggaatcagc gaagcaacat
     1501 gctgggcaat gtcttggacg atatccgagt caccttcggc tgccatctta tgttcaggga
     1561 tggggtaatt gaccggcaca atgtagttca aacggctcac atgaatgaag gaatcaccta
     1621 atgtacgcgg catctgatca ttgacttcag caataatgat cttggcagac tcagccgcag
     1681 acttggtaag ccccacttcc acgccaaggc tgcagaaccc atgttcatct gggcgagaga
     1741 catgaatcag tgccacatcc acaggcagaa tgccacgttt gaatagaagt gggaattctg
     1801 aaagtaatac aggcgtaaag tctgcgcgac catcttggac tgctttacga acattggcgc
     1861 taataaacat ggaattgacg cgtagatgtc cttccatggc ttcagttacg taatcagcag
     1921 cgccaatcgt caatgcctgg caaacttcca catcattgat attgggagca tgccccacca
     1981 gtgcagacaa aagtaccttg gggaccgaga cgttcccggt cagaaagacg cggttgcccg
     2041 atttgacctg cttgatggct tcctctgcag tcgtcacacg ggattgataa atggaagacg
     2101 gattcattcc ttcacctcca cgccacccaa acgtacaagg ttgactaact tcccttgatg
     2161 tgtattgacc gcagcttcaa ttgaagggtc attcttcatg gcggcgtcaa tgcctgtgtt
     2221 cgctattgct gtgatgtagg gaatagctga gttcaaaaac acatggctgg ctgtgcgcgc
     2281 cactgtgctg gcaatattag gaacacagta atgcacaaca ccttcctcaa tgaaagtggg
     2341 gttgtcatgc atggtcgggc gggaggtttc tacacaacca ccttgatcga tactgacatc
     2401 aataatgacc gagcgcggtt tcatagcacg gaccatttcg cgtgtgatca ccatcggtgc
     2461 gcgttgacct gccaccaaaa ccgcccccac aactacgtcc gcataggctg tggcacgttc
     2521 aatgttgcgt tgcgttgcca tcatggttac cacgccaggc aacttatcgt ggatgttttc
     2581 cagcgcagcc atattcttat ctaggacagt cacatgcgca ccggcatttt gcatggcttg
     2641 cgcggcagaa gttcccaccg caccaccacc gataattacc acctcggcag gcggcacacc
     2701 aggtacgcca ctgagcataa tgcccttgcc accgcgattc gtctgcagcc aacgcgccgc
     2761 aatttgagcg accatcgcac cgcagatcaa actaaacgga cgcaaaacgg ctcgtccgcc
     2821 ttgagcatct gtaatctgct cgtatgccag cgctgtaatc tttttctcca agagcaggtc
     2881 aatctgatct tgtgaagcag aagcgagatg taaaaacccc gcaaggatag ctccatcatt
     2941 gatccattcc atttcctgct tgatcgggcg tgagaccttc aataaaaagt ctgcccgccc
     3001 aaaaacttct tgcgcagaaa aggcgatgcg cccgccggct ttttcgtaat cctgatcttt
     3061 gaagccagca ctcgcccccg catcatgctc aacgaaaact tgatgcccca aatgagacag
     3121 aatctccacc ccagctggag atagcccaac tcggtactca aacggtctac tttctttcgg
     3181 aattccaata tacatggttc ctccataaaa agaccctaga ggtatataaa cctttagggt
     3241 ctggtttaat ttattacggc atattgagag tttccctgat cgcagggagc gcccaatcta
     3301 tcgtatcttt atcaataaca agtggtggtg caaaacgtat cacattatca tgcgtttctt
     3361 ttgcaaggat gcccttgctc ttcaatgctt cacagaaacg acgggctccg cctgcttctg
     3421 gtttgagttc cacaccaatg agcaagccct taccacgcac ttccttgaca tgtggactgg
     3481 ggatttcgct caattgctct acaaaataag ttcccaactc tgccgcacgg tctgctaatt
     3541 tttcttcgcg caaaactttc aatgaagcac gagccaccgc cgcagcgagc ggatttccac
     3601 caaatgtgga gccgtgttca ccgggtgtaa acaagcccaa aatgggttta tccgcgagaa
     3661 cagcagagac ggggtaaaag ccaccagcca atgcctttcc tacgatcaca acatccgggc
     3721 ggacgtcttc atgattcgcg gcaaacagtt tcccggtccg accgagaccc gtttgaattt
     3781 catccgagat ataaagtaca ttattcttct tgcaaagctc ggcaactttc ttcaagtatc
     3841 cagccggcgg catgatcacc cctgcctctc cctgaatcgg tt
//
LOCUS       DAOQFT010000205         3837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13257_length_3837_cov_5.372819, whole genome shotgun sequence.
ACCESSION   DAOQFT010000205 DAOQFT010000000
VERSION     DAOQFT010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13257_length_3837_cov_5.372819"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..2528)
                     /locus_tag="PLR93_06370"
     CDS             complement(219..2528)
                     /locus_tag="PLR93_06370"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26626.1"
                     /translation="MEQKIKILFVDDDPEMRTLVSFILKRAGYEVELVESATEAFQYL
                     THSTPNLILSDVMMADSDGFTLLDTLRKQEATRHIPVILLTALAAPSDLVKGLSLGAD
                     DYIQKPFQSAELLARIQSKINRPPMPSELVPMDIRSGLVKPEQFEEEFKKEFFRSLRN
                     GSEGFIAFLSLSELAITRERVGIDIAPEIWKQVARSIAADLGPTDVIGLANGNLLGII
                     LPDMLEATAHKVLVRLTRKIMNYTFVVGNERFRLTPSIGYANFLSAKNAADARNRALT
                     ALDNAIHHLDMEPRLYEPSMGSIEKKEKNSFFQSVVQVLEKLILPFQIGLTFLIGIIL
                     PYFIYRWMDFIGYDITPVAYMIVTISLVLTALLIWVEGFYALRKNPIPETPGAPYPPA
                     TAVIAAYLPNEGATIVDTIEAFLRLDYPAPLQIILAYNSPYEMPIERDLKEIAARDPR
                     FVPFRVSNSTSKAQNINTVIAKVTGEFIGIFDADHHPDADSFTRAWRWLSNGYSIVQG
                     HCAVRNGDFSWVSRLVAVEFEAIYAISHPGRARLHQFGIFGGSNGFWKVDLLRQTRMH
                     GFMLTEDIDSSIRVTMDGVKIASDPDLISRELAPTNLKTLWNQRMRWAQGWHQVSLKH
                     LLQALASKKLTVRQKFGAFWLLGWREAYPWLSMQMFPIILFWIQKYHGVENINWLIPI
                     FVMATLFTLSVGPGQVFFSYRLSLPEIKQHKGWFVFYFIFTSLIYTEFKNTIGRVAQI
                     KELFKERQWKVTPRSAPQPPQGQSRFVSR"
     gene            complement(2692..>3837)
                     /locus_tag="PLR93_06375"
     CDS             complement(2692..>3837)
                     /locus_tag="PLR93_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNH26627.1"
                     /translation="GITLIWMSLILGSLFLGLSFLTGKVQAVFSEEETVISQLARTIF
                     EGRGALYYMLISGTTVILIMAANTAFADFPRLGALHAGDGFLPRQLTYRGSRLVYSRG
                     IITLAVIASILIVIFQASVTRLIPLYAIGVFLSFTLSQAGMARRWWKIGHLKPGEEIV
                     EPGSVLKFEKGWQYRMVINGFGSLCTAIVMVVFAVTKFREGAWVVLILTPILVTIFFS
                     IHHHYKNVANNLSLDNFGAVPPHQTRHRIIIPISGVHQGSLAALRYARMLSDDVTAVH
                     VAIEPAEADKVKKKWELWGEGTRMVMLNSPYRLFLEPMLEYITEISERRQPGETLTIV
                     VPEFISNNMLGAALHTNTADLLRSQLKRQEGVVIINVPYQLREHVKN"
BASE COUNT         1051 a          899 c          955 g          932 t
ORIGIN      
        1 cgccgaactc ttacgcgccc gcggacatta ctatcgccta tatacccagc aattccgcca
       61 tgagttggaa gtgcaatacg gggtggcgga aggagaaaca agtaacgagc gtgaaggggt
      121 aagcgcggat tcgattgtag tgaaacgtga gaagagtgaa actgtggtgg cggattaata
      181 agtaaaagat gtccctgaga gttctcaggg acatcttttt atctactcac gaatcgactt
      241 tgaccttgcg gcggctgagg agccgagcgc ggggtgacct tccactggcg ctctttgaat
      301 aactccttga tctgtgctac ccgaccaatg gtatttttga attccgtata tatcaatgaa
      361 gtaaagataa agtaaaagac gaaccaacct ttatgttgct tgatttcagg caggctgagc
      421 cgatacgaga agaaaacctg acccggtccc acgctcaatg taaatagagt cgccatcacg
      481 aagatgggaa tcaaccagtt aatgttttcc acgccatggt atttctgtat ccaaaacagg
      541 atgatcggga acatctgcat cgaaagccag gggtaggctt cacgccaacc caacaaccag
      601 aaggcgccga acttttgtcg tacggtcagc tttttagatg ccagcgcctg cagtagatgt
      661 ttcaaggaaa cttggtgcca gccttgagcc caacgcatac gttgattcca aagagttttc
      721 aggttggtgg gtgccagttc acgcgagatc aagtctgggt cggaggcgat cttcactcca
      781 tccatcgtga cgcggatcga agagtcaatg tcttcagtta gcataaaacc atgcatgcga
      841 gtctggcgca gcaggtcaac tttccaaaat ccgttcgagc cgccgaagat accaaattga
      901 tgcaaacggg cacgtcccgg gtgactgatc gcatagatcg cctcaaactc gacggcgaca
      961 aggcgcgaca cccatgaaaa atcaccattg cggacagcgc aatgcccctg cacaatgctg
     1021 tatccgttcg aaagccagcg ccatgcgcgg gtaaaactat cagcatctgg gtggtgatct
     1081 gcgtcaaaga tacctataaa ctcgccagta accttggcaa ttacggtatt gatattttga
     1141 gccttggatg tactattgct gacccggaac ggtacaaaac gcggatcgcg tgcggcaatt
     1201 tctttcaagt ccctttcgat gggcatctcg tatggactgt tataagcaag gatgatctgc
     1261 aaaggcgcag gatagtccaa cctcaaaaac gcttctattg tgtcgacaat ggttgcgccc
     1321 tcgttgggca aataggcagc aatgacagcg gtcgctggcg ggtatggcgc cccaggcgtt
     1381 tcagggattg gattctttct cagcgcataa aatccttcca cccagatcag aagcgctgta
     1441 agcaccaacg aaatggttac gatcatatag gctacagggg taatgtcgta accgataaag
     1501 tccatccaac gatagatgaa gtacgggagg ataatgccga ttagaaaggt tagcccaatt
     1561 tgaaagggta ggatcagttt ctccaaaact tgcaccacag actgaaagaa gctgtttttc
     1621 tcttttttct caatagatcc catagacggt tcgtaaaggc gtggctccat atccaagtgg
     1681 tggatggcat tgtccaaagc agttaaggcg cggttcctag cgtcggctgc attttttgca
     1741 gaaagaaaat ttgcatatcc aatcgaaggc gttagacgaa accgctcatt cccaactaca
     1801 aatgtatagt tcatgatttt tcgcgtgagc ctgactaaaa ctttatgtgc agtggcttcc
     1861 agcatatccg gcaggatgat ccccaagaga ttcccatttg ccagaccgat cacatccgta
     1921 ggacccaggt cagcggcgat ggatcgagcc acctgcttcc atatctcagg tgcgatatct
     1981 atgccgacgc gttcgcgagt aatggcgagt tcggataaag ataaaaacgc aataaaccct
     2041 tcagagccat tgcgtaggga tcggaagaac tcctttttaa attcttcttc gaattgttct
     2101 ggtttcacca acccactgcg gatgtccatg ggcaccaact cgctaggcat gggcggacga
     2161 ttgatcttcg attgaattct tgctaatagt tcagccgatt ggaatggttt ctgtatgtaa
     2221 tcatctgcgc ccaaggacaa cccttttact aggtcactgg gggcagcgag agcagtaagt
     2281 aatatcactg gaatatgcct ggttgcctct tgttttctga gtgtatcaag cagagtaaaa
     2341 ccatcggaat ctgccatcat gacatccgac aaaataaggt taggggttga atgggttaga
     2401 tactgaaatg cctcagtcgc actttcgacc aactccactt cgtagcctgc tcttttgaga
     2461 atgaagctta caagggttcg catttccgga tcatcgtcta caaataggat ttttattttc
     2521 tgttccattt tttagctcct tgaaagacaa tctttcaatt tcgcttagaa aataccccaa
     2581 aactatgtat taattccatt agggaaaaca tgtatgaaac aagaaagaca aaaccctcta
     2641 tccattttgt aaggaagagg gttttgtctt tgcagttttt tgtttaatta tttaattctt
     2701 cacatgttca cggagctgat acggaacatt gatgatgacg accccttcct gacgtttcaa
     2761 ctggctacgc aatagatctg ccgtgttggt gtgaagagcc gcacccaaca tattgtttga
     2821 aatgaactca ggcaccacaa tggtgagggt ctcgccgggt tggcggcgtt cggagatttc
     2881 agtgatgtat tccagcatcg gctcaaggaa gaggcggtat ggcgaattca gcattaccat
     2941 gcgtgtcccc tctccccata gctcccattt ctttttaact ttatccgctt cggcgggctc
     3001 aatggcaaca tggacagcag tgacatcatc cgaaagcatg cgcgcgtatc gcagagcagc
     3061 taatgagcct tgatgaacgc cgctgatcgg tatgatgatg cgatgacggg tttgatgcgg
     3121 cggaacagca ccgaaattat caagagagag gttgttcgcg acattcttat aatggtggtg
     3181 gatcgagaag aaaatcgtca caaggatggg ggtaagaatg aggacgaccc atgcgccttc
     3241 acggaacttg gttaccgcaa agacgaccat gacaatagct gtacataagg agccaaagcc
     3301 attaataacc atgcggtatt gccagccttt ttcaaactta agcacagagc ctggctcaac
     3361 gatctcttca cctggcttaa ggtgaccgat cttccaccaa cggcgagcca tgcctgcctg
     3421 tgacaaagta aatgacagga acacaccaat ggcatacaga ggaattagcc ttgttacgct
     3481 ggcttggaag ataacgataa ggatggaggc gatcacagct agagtgatga tgccacgtga
     3541 gtagacgagg cggctgccgc gatacgtcaa ctggcgaggc agaaacccat cgccagcgtg
     3601 aagcgcaccc aaacgcggaa agtctgcgaa ggcggtgttg gcagccatga tgaggatgac
     3661 tgtcgtgcca gagatcagca tgtaatataa agcgcctctg ccctcgaaga tggtacgtgc
     3721 caactgcgag ataacggtct cttcttcaga aaacaccgct tgtacttttc ctgttaagaa
     3781 ggacaaacca aggaagagcg aaccaaggat cagagacatc cagatcaggg tgatacc
//
LOCUS       DAOQFT010000206         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13262_length_3836_cov_6.130389, whole genome shotgun sequence.
ACCESSION   DAOQFT010000206 DAOQFT010000000
VERSION     DAOQFT010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13262_length_3836_cov_6.130389"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLR93_06380"
     CDS             <1..648
                     /locus_tag="PLR93_06380"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HNH26628.1"
                     /translation="PALTQTVKPQSFLVRFFGVYLDPRAWGALFYMLIAFVTGIFYFT
                     WAVTGVSLSLSLMILIFGLPVALLFLISIRGLALLEGRLVEALLGIRMPRRPLFTHQG
                     MKWLERLKALLVDRQTWMMLLYMVLQFVLGIVYFVVIVMVLSFSLAFIALPILQETLH
                     MGAVVINDTRFFMPSWSYPLFVVGGFLLWTIFMNIVRGVGQLHGRLAKWILVGES"
     gene            complement(706..2955)
                     /locus_tag="PLR93_06385"
     CDS             complement(706..2955)
                     /locus_tag="PLR93_06385"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH26629.1"
                     /translation="MVNVIGPDISFYQDDPQTPQGVNFIKMRESAYYVIVRAGQNLWG
                     DRDFKVNWRESKAAGLPRGSYWFYDSRIDPKQQAEKWISMFDGDFGELPLWGDFEDTY
                     NGPYKGWKNWYNFLERLKQLLPSDKEVGVYTGYYYWRENTVAVGIPNASLNYFKQYPL
                     WIANYGTSAPLVPEPWDTWTLWQFTDNGDGPAYGVESLNIDLNYFNGDLEALKTRFKL
                     GDLNFPPAPTPSNYYRVTVSSLKVRDAAGLNSTQIGSITLNEVVEKLEANSDGTWLKI
                     RKKDGSLTGWSFAAYLQNLSSTDSSTDNSGGSNSGGSDSGSGNSGGGTSGGDTGTNNP
                     PDEEDTNWYRVTASSLKIRSGPGLEYDAIGTFTLGELVEKLEATTNNDWLKVRSKDGT
                     KTGWCSGEYLISVHNPSDEQASGTPVDEHADKNWYRVNTVTLNLRETAELNGKILSTL
                     TKNDVIPGLDESNNKWLKTEKLDGTTGWVSRDYLVQVSTASRPSSISQKLFAGVTYLR
                     KDLTSPRQIVVHVFAVDLKELKLEFLVTPSTDNTDVLCTRVTSKFLEEFKLHAAINGG
                     YFSYIETSSSSTSLCPNGGDPVRISDYAASRGKIYSPKKTAQPVVHISRKNVVSFNKA
                     PGAVFNAVAGDRMVVVDGKVVKNLAAQVPNPRTAVGLNKNGRWLTMMVVDGRQPGFSE
                     GVTFPELAELLISYGVHTGANMDGGGSSTMVIRGADGKARVLNSPIEMNQPGKERPVG
                     NHLGLFIKP"
     gene            complement(3017..3370)
                     /locus_tag="PLR93_06390"
     CDS             complement(3017..3370)
                     /locus_tag="PLR93_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNH26630.1"
                     /translation="MTKHNQRVGAWGEETAAVWLAGKGYEVSRRNVRTPYGEIDIIAQ
                     KNGVVYFIEVKTLTSSKEFFPEHQITAKKLEHMLNSAAHYAAELGIDRWQIDVIAIEG
                     KPGVEPRIHHFENIQ"
     gene            complement(3367..>3836)
                     /locus_tag="PLR93_06395"
     CDS             complement(3367..>3836)
                     /locus_tag="PLR93_06395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH26631.1"
                     /translation="EIRDVVKAQMNSDRIHMTGRVPYDQLPAYLAMCDIFATASITEV
                     HPLSVIEAMGAGLPTMGIESVGVGDTVEDGVTGFLATNDMPSFTAKLTRLCLDPELRQ
                     QMRISARKASTAYAIERTTGLMLEKYKHLTSEDFSHKGSFNTRLREILERFLT"
BASE COUNT          937 a          922 c          867 g         1110 t
ORIGIN      
        1 cctgcgttga cacaaacagt caagccgcaa tcttttttag ttcgtttttt cggcgtttac
       61 cttgacccac gtgcttgggg cgcattgttt tacatgttga ttgcatttgt aaccggcatt
      121 ttctatttca cgtgggctgt tacgggtgta tcactttctc tttccttgat gattttgatc
      181 tttggcttac ctgttgcttt gctttttcta atttctattc gagggctagc attgttagaa
      241 ggtaggttgg tagaagcttt gcttggcatt cgtatgccgc gccgtccttt gtttacacat
      301 caaggcatga aatggcttga acgcctcaaa gcattgctcg ttgaccgcca aacctggatg
      361 atgttgcttt atatggtttt acagttcgtg ctgggcattg tgtactttgt tgtgatcgta
      421 atggtactga gtttttctct agcgtttatt gctttaccaa ttttgcaaga aactcttcac
      481 atgggtgcgg tggtcataaa cgatacaaga tttttcatgc catcttggtc gtacccatta
      541 ttcgtggtgg gcggtttcct cttgtggaca atttttatga atatcgttcg cggagtggga
      601 caattgcatg gaagactcgc caagtggatt ttggtgggcg aatcctgata taaggaaata
      661 aaaacagtcc ggctcaaaac cggactgttt tttgtttcct gtttcttatg gcttgataaa
      721 cagccctaag tgattcccaa ccgggcgttc cttgccaggt tgattcattt caatgggaga
      781 gttcagcacg cgcgctttgc cgtctgcgcc gcgaatgacc atggtggagg acccgccacc
      841 gtccatgttt gcgccggtgt ggactccata tgaaattaac aactcagcca actcagggaa
      901 ggtcacgcct tcgctaaacc caggctgacg tccatctaca accatcatcg tgagccagcg
      961 accgttctta ttcaacccaa ccgccgtgcg tgggtttggc acttgagcag cgaggttttt
     1021 cacaactttg ccgtctacaa ctaccatacg gtcacctgcc acggcattaa agacagcacc
     1081 cggcgccttg ttaaaactaa ctacattttt acggctgata tgaacaaccg gttgagccgt
     1141 ctttttcggg gaatagatct tgccacgcga agccgcataa tcgctaattc gcaccgggtc
     1201 gccgccattg ggacataaag atgtagatga actggacgtc tcaatgtaac tgaaatatcc
     1261 gccgtttata gcagcgtgca acttgaactc ttccaaaaat ttagaggtta cgcgtgtgca
     1321 caatacatct gtattatctg tcgatggggt gacaaggaat tcaagcttta gttccttgag
     1381 gtctactgca aagacatgta caacgatctg ccgtggagaa gtgaggtctt ttcggagata
     1441 tgtgacccca gcgaataact tttgagaaat agaactgggg cggctggcag tgctgacctg
     1501 taccagataa tcacgagaca cccagcccgt cgttccatcc aacttttcag ttttgagcca
     1561 tttgttgttt gactcatcca agcccggaat aacatcattc tttgtaaggg tgctaagaat
     1621 tttcccatta agttcagcag tttctcgcag gtttagggtt acggtattaa cccgatacca
     1681 gttcttgtct gcatgttcat caacaggggt tccagaggct tgttcgtctg atggattatg
     1741 aacgctaata agatactcac cagaacacca gccggttttg gtcccatctt tactgcggac
     1801 tttaagccag tcattgtttg tagtagcttc tagcttttct acaagttcgc ccaacgtaaa
     1861 ggtgccgatg gcatcatatt ccaaaccggg acctgagcgg atcttcaacg aagacgccgt
     1921 cacacgatac caattggtat cttcctcatc tggcggattg tttgtaccgg tatcgcctcc
     1981 gctggttccg ccaccgctat ttccagaccc agagtcgctg ccgccagaat tcgaaccgcc
     2041 agaattatcg gtagaagaat cggtgctgct caaattttga agataggcgg caaaactcca
     2101 ccccgtgagg ctgccatcct ttttacggat cttaagccag gttccatctg aattggcttc
     2161 gagtttttct actacttcgt ttagggtaat gctcccaatc tgagtggaat tcaaacccgc
     2221 tgcatcgcga accttaagtg aagaaacggt cacgcgataa taattactgg gggtaggggc
     2281 tggagggaag ttaaggtctc ccagtttgaa gcgggttttg agagcttcca gatcaccatt
     2341 gaaatagttc aaatcaatat tgaggctttc aaccccatac gcaggaccat ctccattatc
     2401 cgtgaattgc caaagcgtcc atgtatccca aggctcagga acgagcggag cacttgtccc
     2461 ataattggcg atccataatg gatattgttt gaaataattc agcgaagcat ttggaattcc
     2521 gaccgcaact gtattttcgc gccagtagta atacccagtg taaacaccaa cttctttatc
     2581 agaagggagc aactgtttca acctctctaa aaagttgtac cagtttttcc aacctttata
     2641 aggaccattg tacgtatctt caaaatcgcc ccagagtggc aattccccaa aatcaccgtc
     2701 gaacatcgat atccattttt cagcttgttg ttttgggtca atgcggctgt cgtaaaacca
     2761 ataagaaccg cgtggcaaac cggctgcttt tgactcacgc caattgactt tgaaatctct
     2821 atcgccccat aaattttgcc ctgcacgcac gatcacataa taagcgcttt cacgcatctt
     2881 aataaaattg acgccttgcg gagtctgtgg atcgtcctga taaaaactaa tatcgggtcc
     2941 gatgacattt accatgagag tctcctcgtt gttcgttagc cgtatgcttt caggttatat
     3001 aaattatatc tgatacttat tgaatatttt caaagtgatg aatgcgcggt tctacgccgg
     3061 gtttcccctc aatcgcaatt acatcaattt gccagcggtc aattccaagt tcggcggcat
     3121 aatgagccgc tgagttaagc atatgttcca gcttttttgc agtgatttga tgttcaggga
     3181 aaaattcttt cgaagaagtt agcgtcttga cctctataaa atagacgacg ccatttttct
     3241 gggcgatgat atcgatctcc ccatacgggg tgcggacatt tcgacggctc acttcgtagc
     3301 ccttgcctgc cagccagacc gcggcagtct cttcgcccca agcgcccact cgctgattat
     3361 gcttggtcat gtcaaaaagc gttccaaaat ctctcgtaga cgagtgttaa acgagccctt
     3421 atgtgaaaaa tcttcactgg ttaaatgttt atatttttcg agcatcaagc cagtggtgcg
     3481 ttcaatggca taggcagtag aagccttacg ggcagagatg cgcatttgct gacgcagttc
     3541 cgggtccaga cagaggcgtg taagttttgc cgtgaacgat ggcatatcat tcgttgccaa
     3601 aaagcccgtc actccatctt ccaccgtatc cccaacaccg acagattcaa tgcccatcgt
     3661 tgggagcccc gcccccatgg cttcaattac agaaagcgga tgaacttctg taatagaggc
     3721 tgttgcaaaa atatcacaca tagcgaggta ggcagggagt tgatcgtacg gcactctgcc
     3781 tgtcatgtga atacggtctg aattcatctg ggcttttact acatctcgaa tctctt
//
LOCUS       DAOQFT010000207         3822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13347_length_3822_cov_6.437749, whole genome shotgun sequence.
ACCESSION   DAOQFT010000207 DAOQFT010000000
VERSION     DAOQFT010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13347_length_3822_cov_6.437749"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLR93_06400"
     CDS             complement(<1..605)
                     /locus_tag="PLR93_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNH26632.1"
                     /translation="MKRFIAIAGNIGVGKSTLVQMLCNQMGWDPFYEPVTQNPYLADF
                     YKDMSSWAFHSQVFFLTHRLRSHLNLAQHPNSVIQDRSVYEDAEIFAHNLYQQGNMTD
                     RDYQTYRDLYETAVQFLPPPDLVIYLRASVDTLLNRINHRGRDYERTIAPDYLQSLND
                     LYEGWIDNFTLCPVLAVPADDLDYVAHNGHLRLIVSKIEAKL"
     gene            complement(724..1560)
                     /locus_tag="PLR93_06405"
     CDS             complement(724..1560)
                     /locus_tag="PLR93_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNH26633.1"
                     /translation="MNTKSTRSKKSTPAVIEIEQEEPVIESTPDSEDFITFKRSHFYS
                     VLVVLAFAVGLLLGFVIWGRGSNTVTVAADPAAAANNAAAQQPSGEIAEAPVTPQPQY
                     TRYDIPTEGYPSLGPDDAPITIVEFSDFQCPFCRRFHSETYQALLDAYPGQIRFVYRN
                     LPLTSIHPDAMPSAIASLCANDQNVYWEYHDKLFSSETLTRDTFIQYATDLNLNVDEF
                     SACLDSGKHDENIQQDMDFALNLGVQSTPTFFVNGLAIVGAQPLSSFTQIIDMELAGE
                     IP"
     gene            1681..1911
                     /locus_tag="PLR93_06410"
     CDS             1681..1911
                     /locus_tag="PLR93_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26634.1"
                     /translation="MTTTTQKRQIPWYLWPFAAIWKLLAVIVEMTGRLVAMILGIVLL
                     VVGVLVSLTIVGAIVGVPLGIVGLLLFFRGMF"
     gene            complement(1949..2629)
                     /locus_tag="PLR93_06415"
     CDS             complement(1949..2629)
                     /locus_tag="PLR93_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNH26635.1"
                     /translation="MKIFNRYTPLQIAIHLYAWSALVWIIAELFMGTFSINPIQELEQ
                     RTGRHAITLLVLSLLCTPLNTIFKFPELLKRRRTLGLYALLYATIHVLIFINLDYGLA
                     WSMIARTVFEKPYIIVGALAFLMLIPLGITSFDIWKVRLGKNWKRLHQMVYVIAPLVV
                     LHYLWSKKGDLLNFSGDIVRPTIYGLVVVIFLILRIPAIRRAIASLPNRGLTLPFKKN
                     PTPKADAS"
     gene            complement(2678..3625)
                     /gene="msrP"
                     /locus_tag="PLR93_06420"
     CDS             complement(2678..3625)
                     /gene="msrP"
                     /locus_tag="PLR93_06420"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNH26636.1"
                     /translation="MSHRFKSVPVRSSEITPKEVYLSRRDFIKAAGVVAGSMALAACA
                     PESSATPAAQFNGPVLNDELGDPTNTFEEITNYNNYYEFTTNKEAVAPMAKDFTTTPW
                     TIEVSGMVSKPKTFGLEDLLSMFPQEERIYRLRCVEAWSMVIPWNGFPLASLLKAVEP
                     TSNAKYVRFETIYRPEEMKGQSSPFYPWPYQEGLRLDEAMNDLTLLATGIYGETILPQ
                     NGAPIRLVVPWKYGFKSIKSIVKIELTDKEPTTLWSTIAPNEYGFYANVNPTVDHPRW
                     SQASERRIGELTRRETLMFNGYGEQVADLYADMDLNLNY"
BASE COUNT          938 a          792 c         1080 g         1012 t
ORIGIN      
        1 agtttggctt cgatctttga gacaattaag cgtaggtgac cgttatgggc aacataatct
       61 aggtcgtcgg ctgggacggc aaggacgggg cagagggtga agttgtcgat ccaaccttcg
      121 tagaggtcat tcaggctttg cagatagtcg ggagcgatgg tgcgttcata gtcacgcccg
      181 cggtggttga tgcggtttag gagggtatct acagaggcgc ggaggtaaat aacgaggtct
      241 ggtggcggca ggaattgaac ggcagtttcg tacaggtcgc ggtaggtttg ataatcgcgg
      301 tctgtcatgt tgccttgttg gtagagattg tgggcaaata tctcggcatc ttcatacacg
      361 ctgcggtctt ggatgacaga attgggatgt tgcgccaggt tgaggtgaga gcggaggcgg
      421 tgagtcaaaa agaagacttg ggaatgaaac gcccaagaag acatgtcctt gtaaaaatcc
      481 gcgaggtatg ggttttgggt gacgggctca tagaatggat cccagcccat ttggttgcaa
      541 agcatttgta cgagcgtgga ctttcctaca ccgatatttc ccgcaatggc gataaatcgt
      601 ttcatggcat tctctctgag tggtttatta tttacatctt accactggga taaattttgg
      661 cacggagttt taagaaaaaa ggcggacgat accgtccgcc ttttaagatt gtatttctaa
      721 agcttagggg atttcacccg caagttccat atcgataatt tgggtgaagg aagaaagcgg
      781 ctgcgcgccc acaattgcta aaccgttaac aaagaaggtg ggggtagatt ggacgccgag
      841 attgagagcg aagtccatat cctgctggat gttttcatcg tgcttgccgc tatcgagaca
      901 ggcagagaac tcgtctacgt ttaggttcaa atcggtggca tattgaataa aggtatcgcg
      961 cgttagagtt tcgctgctga agagtttatc gtgatattcc caatacacat tctggtcatt
     1021 tgcgcagagg gaagcaatcg cggagggcat cgcatcggga tggatggagg tgagaggtag
     1081 attgcgatat acgaagcgga tctgaccggg ataagcatcc aacaatgcct gatatgtttc
     1141 cgaatggaag cggcggcaga aaggacactg gaagtcactg aattctacga tggtgatcgg
     1201 cgcatcatct ggacctaggc tggggtagcc ctcggtgggg atatcgtagc gggtgtattg
     1261 tggttgcggg gtaacaggcg cttcggcgat ttcgccggaa ggttgttggg ctgcagcatt
     1321 attggcagca gcggctgggt ctgcagcgac ggtaacggtg ttactgccgc gtccccaaat
     1381 gacgaagcca agtaagagac caacagcaaa ggcaaggacg actagcacag agtaaaaatg
     1441 actgcgcttg aaggtgatga aatcttcaga gtctggcgtg ctttcgatga caggctcttc
     1501 ttgttcgatt tcgatcacgg cgggcgtgga ttttttagac cgggtagatt ttgtgttcat
     1561 agtggcaaga ttatagtcct gtgttttgga tttaagcagt agtacttaag tatttttcat
     1621 tctacttttt agagaaccat ttgtcctatt ttgcgtatat taggcatgga ggttcttctt
     1681 atgactacaa caactcagaa aagacaaatt ccttggtatt tatggccctt tgccgcaatc
     1741 tggaaattgc tggctgtcat tgtagagatg actgggcggc tcgtggcaat gatcctcggt
     1801 attgtgctgc ttgtcgttgg cgttctcgtc agcctgacga ttgtcggtgc gattgtgggc
     1861 gtgcccttgg ggattgtcgg tttactgctg ttctttcgtg ggatgtttta aagtgtaagg
     1921 gcgacctgtc aggtcgccct tatttaaatt aagaagcatc cgctttgggg gtgggattct
     1981 ttttgaaggg gagagtcaag ccgcggttcg ggagagaggc gatggcacgt cgaatggcgg
     2041 gaatgcgcaa aatcaggaag atcacaacga ccaagccata gatcgtgggg cgcacgatgt
     2101 cgcctgagaa attaagtagg tcgccttttt tgctccacag gtaatgcagc accaccaagg
     2161 gcgcaatgac atataccatt tgatgaaggc gtttccaatt tttgccgagg cggaccttcc
     2221 aaatatcgaa tgaagttatg ccaaggggaa tgagcatgag aaatgccaat gcaccaacga
     2281 tgatatacgg cttttcgaaa acggtgcgag cgatcatgct ccatgctaga ccataatcga
     2341 gattgataaa gattagcaca tggatggtgg catataacaa agcatataag cctaaggtgc
     2401 gacggcgttt gagcagctca ggaaatttga atatcgtgtt gagcggggtg cataataaag
     2461 acagcaccaa aagagtaatg gcatgccgcc ctgtgcgttg ttccaactct tgaatggggt
     2521 tgatcgagaa cgtccccatg aacagctcag caatgatcca taccagggca gaccaagcgt
     2581 aaaggtgaat ggcaatttgt aaaggggtat agcgattaaa gattttcatg accttagtaa
     2641 acgtcggaag tcttgtggct tccgacgttt catttcttta gtaattcaag ttcaagtcca
     2701 tatcggcgta taagtctgcg acttgttcgc cgtagccgtt gaacatcaac gtttcgcggc
     2761 gggtcagttc gccaatgcgg cgttcagacg cttgtgacca acgtggatgg tcgacagttg
     2821 ggttgacgtt ggcatagaag ccatattcat tgggcgcaat cgtgctccac aatgtggtgg
     2881 gctctttgtc ggtcaattcg atcttgacga tggacttgat ggatttgaag ccatatttcc
     2941 acggtacaac caaacgaatc ggcgccccgt tttgagggag aatggtttca ccgtaaatac
     3001 cagttgccag caaggtcagg tcgttcatgg cttcatcgag gcgtaaccct tcttgatagg
     3061 gccatgggta gaacgggctg ctttgtccct tcatttcttc ggggcgataa atggtctcaa
     3121 agcgcacata ctttgcattg gacgtgggtt caacggcttt caacaaactc gcgagcggaa
     3181 acccattcca tggaatgacc atgctccagg cttctacgca gcgcaggcga taaatgcgct
     3241 cttcctgtgg gaacattgaa agtaagtctt ccaaaccaaa agtcttcggc ttgcttacca
     3301 taccgcttac ttctatggtc cacggcgtgg tggtgaagtc ttttgccatg ggcgctacgg
     3361 cttctttgtt ggtggtgaac tcgtagtaat tgttgtagtt ggtaatttct tcaaaagtgt
     3421 tggtggggtc acccagctca tcgttcagaa caggaccatt aaattgagcc gctggggttg
     3481 cagaggattc tggggcgcaa gcggcaagtg ccatactgcc agcaaccaca cccgcagctt
     3541 tgataaagtc acgccgagag agatacacct ctttgggggt gatctcagaa gaacggacag
     3601 gtacagattt aaaacgatga ctcatggagg tctctcctcg ttcaatgatt tactttaatg
     3661 gacacactga ttctaaaaaa actttctaac ggacagatga taatttgctt aggaaatctt
     3721 cagttggaaa cttgcgcctc tttcgtagcc ggtagttgct tcgcttcggt aaagcgtcac
     3781 ctcaccgccg ccgtagactc cccattgtac ttctgttccg ag
//
LOCUS       DAOQFT010000208         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13374_length_3817_cov_5.992026, whole genome shotgun sequence.
ACCESSION   DAOQFT010000208 DAOQFT010000000
VERSION     DAOQFT010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13374_length_3817_cov_5.992026"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..651)
                     /gene="rfbC"
                     /locus_tag="PLR93_06425"
     CDS             complement(103..651)
                     /gene="rfbC"
                     /locus_tag="PLR93_06425"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNH26637.1"
                     /translation="MEFIPTRIPEIILVKPKIYEDPRGYFMEVYREDRFSIEGISRNF
                     VQENQSRSQKGVLRGLHYQIRQAQGKLVRVLSGEIFDVAVDIRRSAPTFGQWIGAILS
                     SENRHQLWIPEGFAHGFYVTSDSAEVAYKVTDYYAPEWERSILWNDPQIGVDWQLAKD
                     AAPILSQKDLNGKPLTDAELFK"
     gene            770..1294
                     /gene="tpx"
                     /locus_tag="PLR93_06430"
     CDS             770..1294
                     /gene="tpx"
                     /locus_tag="PLR93_06430"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNH26638.1"
                     /translation="MTTIRNDVLKLGDKFATLFGDDVKVGQAAPEFTSVVAGWAAVNP
                     IQESKGKVIILSAVPSLDTDVCDRETRRFNEEAAKLGDDIVIYTISTDFPMAQKRWCG
                     AAGVDKVKVVSDVLEAEFGIKYGLLIKERRFLRRAVFIVGRDGKLTYVNYLPTLGNEP
                     DYDEVIAEAKKALG"
     gene            1400..2746
                     /locus_tag="PLR93_06435"
     CDS             1400..2746
                     /locus_tag="PLR93_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HNH26639.1"
                     /translation="MSDKSVIIIGAGMAGLSAAHEFNKAGWAVTVLEARSRVGGRVHT
                     VREFSHGLVAEGGAEYINESQHRMIALAKEFDLYLGAVGSWQNQSGDWAALQGKAGYL
                     HDTALWGADLDAENTKVWVALAELGKQVTDLSNPAASKNAKALDSQNAESWLQNLNVH
                     PLAKVLFANHIRSEYTCEPKDFSLLDLARNAAMYYSDPNASDPAYRIIGGNDQLPQAM
                     AKRLPNVRMNAVVTSIKLLDEKVEVVYKLADTFHTLQASYAILATPLTTARLIDFEGT
                     LPPAHHAMVHELSYGSVTKVMIEYKKRFWHDRGWNGRLNTDLPIVFTWDATSHLEGEH
                     GILTAYTGGEPGAALTRLSDADRIKTAVSAIESIFPGSSNLIENTQTIAWRNEAFTLG
                     SYMAYAPSQVTSHWQALFSPAGRLYFAGEHATIYQGFMEGAVESGQRAAKSIIEKG"
     gene            2750..3454
                     /locus_tag="PLR93_06440"
     CDS             2750..3454
                     /locus_tag="PLR93_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNH26640.1"
                     /translation="MSEARRNFIEGVRAEVPILIGVFPFGLIYGALAINSGLSTTAAQ
                     LMSSIVFAGSAQFITAQLFSEGAPAFVMILTIAVVNLRHMLYSASLAPYLKDLSLRWK
                     VLLSYLLTDEAYAPSILHYEKEGLAKYKYWFLFGAGLSLWINWQISTAIGVFLGAAIP
                     ENLSLDFALPVTFIAMIVPVMKKFPVIAAALSAGITALLAYHLPYKLGLILAALVGIT
                     VGMLLENLQAAQKEAK"
     gene            3451..3771
                     /locus_tag="PLR93_06445"
     CDS             3451..3771
                     /locus_tag="PLR93_06445"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNH26641.1"
                     /translation="MNNIWLVMIVGGLITFSMRFSLIYLFGKFEIPESLRRALHYVPP
                     AVLSAIIFPEIFLSNGALDISLSNARLPAGILAIVAAWYSRSTLVTILIGMAALFFFQ
                     WIKL"
BASE COUNT         1010 a          895 c          871 g         1041 t
ORIGIN      
        1 gttaacattg gtttctttct ggatcaaata atggatgaaa ttcgagccta tgaacccggc
       61 gccgcctgta actagaacat ttttcattct ttactattct ctttatttga acaactcagc
      121 atctgtcaga ggttttccat ttaagtcttt ttgagaaaga ataggtgcgg catctttcgc
      181 aagttgccag tcaacaccga tctgcgggtc gttccataaa atactacgtt cccattcagg
      241 ggcgtaataa tcagttactt tataggcaac ttcggcagaa tcactggtga cataaaaacc
      301 atgagcaaat ccttcaggga tccacaattg gtggcggttt tctgaagaaa ggatcgcccc
      361 aatccactga ccgaaagttg gcgcactacg gcgaatatcc acagctacat caaaaatttc
      421 gcccgaaaga actcgaacca atttcccctg tgcctggcgg atctggtaat gtaagccgcg
      481 caaaacacct ttttgcgaac gagattgatt ttcttgaaca aaatttctgg agattccttc
      541 aatagaaaat cgatcctccc ggtaaacttc catgaaatat ccacgcgggt cttcatatat
      601 tttgggctta actaggataa tttcagggat ccgagtaggg ataaattcca tattttgcta
      661 aaactccatc ttattttttt ctctattata tctgtaatca attccagtaa gggcgggcat
      721 aaaagtagcg tctgataaaa ttgaagaaac ctatttggag aaaaccgaaa tgactactat
      781 tagaaatgat gttcttaagc tgggcgataa attcgccacc ctttttgggg atgatgtgaa
      841 agttgggcaa gcggcaccag aatttaccag tgttgtagca ggttgggcgg ctgtaaaccc
      901 aattcaggaa tcgaaaggaa aggtgattat cctttcggct gttccctcgc tggatacaga
      961 tgtctgtgac cgtgaaaccc gtcgctttaa tgaagaagcc gctaaactgg gtgacgatat
     1021 tgtgatctac accatcagca cagactttcc catggcgcaa aagcgctggt gcggagccgc
     1081 cggtgtggac aaggtcaagg tcgtgtctga tgtacttgaa gctgagtttg gcatcaagta
     1141 tgggctgttg atcaaggaac gccgcttttt acgtcgcgcc gttttcattg taggacgtga
     1201 tggtaagtta acctatgtca actacctacc tactttaggg aatgaacccg attacgacga
     1261 agtgattgca gaagcaaaga aagctctcgg ctaaataaaa gccgaatggg cagtaacaag
     1321 cccaagtctt gactatgagg cgaagccgtc ctgcatgttg ggacggcttt ttatatccta
     1381 atgataaaat ccacaaacta tgagcgataa atctgtcatc atcattggcg caggcatggc
     1441 gggactttcc gccgcgcacg aattcaacaa agcaggttgg gctgtcaccg tattggaagc
     1501 acgttcacgt gtaggcggac gtgttcacac cgtgcgtgaa ttttcgcatg ggcttgtagc
     1561 agaaggcggc gcagaataca tcaacgaaag tcagcatcgc atgatcgcgc ttgcaaaaga
     1621 gttcgacctt tacttggggg ctgtaggctc ctggcaaaac caaagtggag attgggcagc
     1681 cttgcaaggc aaagctgggt atttacacga tacggctttg tggggtgcag accttgatgc
     1741 agaaaacaca aaagtttggg tggcactggc agaacttgga aaacaagtaa cggatctttc
     1801 aaaccctgcg gcatccaaaa atgcaaaagc tttagatagt caaaatgcag agagttggct
     1861 tcaaaacctg aacgttcatc ctttggcaaa ggtgctattt gcgaatcata ttcggtcgga
     1921 atatacatgt gaaccaaaag atttttctct tttagatttg gcacgtaatg ccgccatgta
     1981 ttacagtgac ccaaatgcct ctgacccagc atatcgcatc attggtggta atgaccaact
     2041 tccgcaagcg atggcaaaac gattgccaaa tgtgcggatg aatgctgttg taacctccat
     2101 caagctgttg gatgaaaaag ttgaagttgt ttacaaacta gctgacactt tccatacgtt
     2161 gcaagcttca tacgctattc ttgcaacgcc tcttacgact gcacgcctga tcgacttcga
     2221 agggacttta cctcctgctc accacgccat ggtccacgaa ctttcctatg ggtccgttac
     2281 caaagtgatg atcgaatata aaaagcgttt ttggcatgac cgcggctgga atggacgcct
     2341 caatacagac ctgccgattg tcttcacatg ggatgctacc agccaccttg aaggcgaaca
     2401 tggaatcctc acggcttaca caggcggcga acccggtgcc gccctaactc gcctttccga
     2461 tgcagaccgc atcaaaacgg cagtctctgc aattgaatct attttccctg gttcttcaaa
     2521 cttaatcgaa aatacccaaa ccattgcctg gagaaacgaa gcctttacat tggggtctta
     2581 catggcatat gcgcccagcc aagtcacctc tcactggcaa gcgctcttct cccctgccgg
     2641 gagactatat tttgccggcg aacatgccac catctatcaa gggttcatgg aaggggcagt
     2701 ggaaagcggt caacgcgcgg caaaaagcat tatagaaaaa ggataaagaa tgagtgaagc
     2761 aagaaggaat tttattgaag gcgtgcgtgc agaagtgcct atcttgatcg gtgtgtttcc
     2821 gttcgggctg atttatggtg cattggctat caattcaggg ttatctacaa cagccgcaca
     2881 gttaatgtcc tccattgtat ttgccgggtc ggcacaattc attacggcgc agttatttag
     2941 cgaaggcgcg cccgctttcg tcatgatcct taccattgca gtcgtcaatt tacgccatat
     3001 gttgtacagt gcgtctcttg cgccctatct caaagattta tcgttacgtt ggaaggttct
     3061 tctttcttac ttattgacag acgaagccta tgctccgtct attttgcatt atgaaaaaga
     3121 agggcttgca aaatacaaat actggttctt atttggcgcg ggtctctctc tctggataaa
     3181 ctggcagatc agcacagcca ttggcgtgtt ccttggtgca gccatccctg agaacctatc
     3241 tctcgatttc gctctgccag ttacattcat cgccatgatc gtgcccgtga tgaaaaagtt
     3301 tccagtgatt gccgccgcat taagtgcagg catcactgct ttactagctt atcatctgcc
     3361 atataaactc ggtttgattc ttgccgcctt ggtgggcatt acggttggaa tgttgcttga
     3421 aaatcttcaa gctgcccaaa aggaggcaaa atgaacaaca tttggctggt catgatcgtc
     3481 ggtggcttga tcactttcag catgcgtttt tccctcattt atttgttcgg aaaatttgaa
     3541 attcctgaaa gcttacgccg cgccttacat tacgtacccc ctgccgtgct ttctgccatt
     3601 attttccccg aaatcttttt aagcaacggc gcgctggata tttctttatc caatgccagg
     3661 ctacctgctg gtatcttagc aatcgtcgcc gcatggtact cacgcagcac actcgtcact
     3721 attctgattg gaatggcagc tctatttttc tttcaatgga tcaagctata gaaaggacaa
     3781 atacacctga ttgccccccg cctcttttgc acgcgtc
//
LOCUS       DAOQFT010000209         3800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13461_length_3800_cov_7.814686, whole genome shotgun sequence.
ACCESSION   DAOQFT010000209 DAOQFT010000000
VERSION     DAOQFT010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13461_length_3800_cov_7.814686"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLR93_06450"
     CDS             complement(<1..440)
                     /locus_tag="PLR93_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH26642.1"
                     /translation="MNTKFSKLMNTKKKDDQHIPPIAGFVFGIILIGSIKVALILDAN
                     HASDLITAAVMLAMIGIASYFLFAIKVASQWEKAVVLRLGKFNGLKGPGLFWLIPIID
                     TAVMWIDHRVTVTSFSAEKTLTKDTVPVDVDAVLFWVVWDAEKAA"
     gene            complement(466..888)
                     /locus_tag="PLR93_06455"
     CDS             complement(466..888)
                     /locus_tag="PLR93_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNH26643.1"
                     /translation="MTNTKLTLHLDFHSGLPIYTQIVNQIQSQLANGILKPGDQLPTV
                     RALASELRVNFNTVARAYRILDEARIISTQQGRGTFITEIPPPEVTEKLRQESLSELT
                     NRFISEAFRLGFSEREVSQMVRDRLKSWKEAKQVKEEE"
     gene            complement(1012..2487)
                     /locus_tag="PLR93_06460"
     CDS             complement(1012..2487)
                     /locus_tag="PLR93_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNH26644.1"
                     /translation="MRIPLDHKSDIPLYQQIEDHLRKSILSGNLPADTRLPASRQLAR
                     DLGVNRTTVENAYSALEADGLVFSKMGSGTYVLPVYPIPSIPKNNSDALPLWQMNLQK
                     NVFATSDTAEDMLQAGGHPRPINFASGISDARQFPVEEFRKVIQSVMRRDQIDALEYG
                     ERRGYAPLREGIARILASQGLQTHPENVLITAGSQQAIFLASQVLLKPNDIVLVEDPT
                     YSAALDLFRTLGFQIVGIPVDSQGMQVEKLEKLLQQYHPKLIYTIPNFHNPTGTCLSS
                     ARRRELILLAGKYNVPILEDDFVGDLRYEGHTQPSLKSLDPGGQVIYISTFSKMLMPG
                     LRVGFIVADGPVYDSLLNFKRLSDLATSTLIQRALDAYMTVGRYQTYLHRSNQIFRSR
                     RDAMLDAIKKHLPSTINFELPKGGLFIWLQLPKSISANELLPVACKEGVAFSPGNSFF
                     IDGISGEDWIRLNFASQPVEDIEEGIKRLGNALKKMKPKKK"
     gene            2676..3563
                     /gene="pdxS"
                     /locus_tag="PLR93_06465"
     CDS             2676..3563
                     /gene="pdxS"
                     /locus_tag="PLR93_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNH26645.1"
                     /translation="MQAKTGTWTEKKGLAQMLKGGVIMDVVNAEQAKIAEDAGACAVM
                     ALERVPADIRANGGVARMSDPDMILKIMEAVSIPVMAKCRIGHFVEAQILESIGIDYI
                     DESEVLTPADEDHHILKHNFKVPFVCGCRNIGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARTVIGDIKKLTVMGDEELMAFAKNNGAPYELVKEVKELGRLPVVNFAAGGVAT
                     PADAALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKSVTHYQDASILAEVSKNLGEAM
                     VGRNVSQMAEGEKIAGRGW"
     gene            3611..>3800
                     /locus_tag="PLR93_06470"
     CDS             3611..>3800
                     /locus_tag="PLR93_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNH26646.1"
                     /translation="MKIGVLALQGDFAEHVSMLKRVGVEAAEVRLPKHLDDLNGLIIP
                     GGESTTIGKLAVAYNLIDP"
BASE COUNT          978 a          836 c          975 g         1011 t
ORIGIN      
        1 gcggcttttt ctgcatccca cacaacccag aacaatacag catccacatc cacaggtaca
       61 gtgtctttgg tcagagtttt ttctgcagag aaagatgtga ccgtaacacg atggtcgatc
      121 cacatcacag cagtatcaat gatcggaatg agccaaaaca aaccgggtcc cttaagccca
      181 ttgaatttac cgaggcgcag aacaacagcc ttttcccatt gagatgccac tttgatcgcg
      241 aacaaaaaat aagaggcaat cccgatcatg gcgagcatga ccgcagctgt aataagatcc
      301 gatgcatgat tagcatctaa aattaacgct actttgattg agccaattaa gatgatccca
      361 aacacaaacc cggcaatggg cggaatatgt tggtcatctt ttttctttgt attcatcaac
      421 ttactaaact ttgtattcat atgtgcattc tcctaacttg aaactttatt cttcctcttt
      481 gacttgcttc gcttctttcc acgacttcaa acggtctctc accatttggc ttacttcacg
      541 ttcagagaaa cccaaccgaa atgcttcact gataaatcga ttcgtaagtt cagagagtga
      601 ctcttgccgt aacttctctg tcacttctgg tggcggaatc tcagtgatga acgtgccgcg
      661 tccttgttgt gtggatatga ttcgtgcttc atccagtatg cggtaagcac gtgctactgt
      721 attgaagttg actctcaatt cagatgccaa tgcgcgcacg gttggtaatt gatccccagg
      781 tttcaaaata ccgtttgcca gttggctttg tatttgattc acgatctggg tatagattgg
      841 taagccggaa tgaaaatcga gatgtaaggt gagtttggtg tttgtcatag aggactctga
      901 attgtactaa ttgtatcaat gattgaattg tagcacttaa ggactttaag tcaagcccct
      961 taagaataaa aaggactttg aggaaattct caaagtcctt ttcgcacaga tttatttttt
     1021 ctttggcttc atctttttca aggcgtttcc caatcgctta atcccctctt ctatatcttc
     1081 tacaggttgc gatgcaaagt tgagacggat ccaatcctct ccactgatgc cgtcaataaa
     1141 gaatgaattg ccgggcgaaa aagcgacgcc ttctttgcaa gcaactggaa gcaattcatt
     1201 cgccgaaatg gacttgggca attgcagcca aataaataaa ccgcctttcg gtagctcaaa
     1261 attaatagtg cttggcaaat gttttttgat ggcatccaac atcgcatctc gccgtgaacg
     1321 gaagatttga ttggagcgat gcaaataggt ttgataacgc cccaccgtca tataggcatc
     1381 gagcgcacgt tgaatcagcg tggaagttgc caagtcgctg agacgtttga aattgagcaa
     1441 actatcgtag acaggtccat ctgcgacgat gaaaccgacg cgcagaccgg gcatcagcat
     1501 tttcgaaaag gtgctgatgt agatgacttg cccacctggg tctaatgact tgagcgaagg
     1561 ttgtgtgtga ccttcatagc gcaagtcacc tacgaaatca tcttcaagaa ttggaacgtt
     1621 gtatttgcct gccaatagaa tcaactcgcg gcggcgggca ctgctgagac aagtgccagt
     1681 tgggttgtgg aaattcggaa tcgtgtaaat cagtttggga tgatattgct gtagaagttt
     1741 ttctagtttc tctacttgca tgccttggct atcgacagga ataccgacta tctgaaaacc
     1801 taatgtgcgg aagagatcca atgcagcaga gtatgtgggg tcttcaacga gaacaatgtc
     1861 attgggcttc aacaagactt gtgatgcgag aaagattgct tgttgtgagc cagcagtgat
     1921 gagaacattt tcgggatgag tctgcaagcc ctggctggcg aggatgcgcg ctattccttc
     1981 acgcaaggga gcatagccgc ggcgctcgcc atattcaagc gcatcgattt ggtcacgtcg
     2041 catcacagat tgaatcactt tgcggaattc ttctaccggg aattgacgtg cgtcactgat
     2101 gccgctggca aagttgatgg gacgtggatg cccccctgct tgtagcatat cttcggcagt
     2161 gtctgacgtg gcgaaaacat ttttctgcaa attcatttgc cagagtggca acgcatctga
     2221 attgtttttc ggaatggatg ggatcggata gaccggtaac acataggtac cgctgcccat
     2281 ttttgaaaat accaaaccat cagcttccaa agcggagtag gcgttttcta cggtggtgcg
     2341 attgaccccc agatcacgtg caagttgacg acttgcgggc aaacgtgtat cggctggcaa
     2401 gttgccggaa agaatacttt tgcgaagatg atcttcgatc tgttgataaa ggggaatatc
     2461 acttttgtgg tcgagcggga tgcgcatttt gtctctccga accgcattgg tacggtgaat
     2521 atatcaccaa cttgtctgct gaataaggac aattcgcttt cttagcgttt tattgggcaa
     2581 aagtggctgg taatttattg atgtttattg gctcttaaca tcagccaatt tcgtcctaga
     2641 atccaatcaa aacatattct atgtaggaga gaactatgca agcaaaaact ggaacttgga
     2701 ccgagaaaaa aggattggcg caaatgctca agggcggcgt catcatggat gtggtgaatg
     2761 ctgaacaggc aaagatcgct gaagatgctg gcgcctgtgc ggtgatggca ctcgagcgcg
     2821 tgccagcgga cattcgcgcg aacggcggcg tggctcgtat gagcgacccc gacatgattc
     2881 tcaagatcat ggaagcagtt tccattccgg tgatggcaaa gtgccgcatc ggtcatttcg
     2941 tggaagcgca gattcttgag tcgattggca ttgactacat cgacgaatct gaagtgctga
     3001 ctcctgccga tgaagatcat cacatcttga agcacaattt caaagtgcca ttcgtttgtg
     3061 gatgccgtaa catcggtgaa gcgcttcgtc gtattggcga aggtgccgcg atgattcgca
     3121 ccaaaggtga agccggaacc ggtgacgtgg tagaagccgt gcgccatgct cgtactgtga
     3181 tcggtgacat caagaagttg accgtgatgg gcgatgaaga attgatggcg tttgccaaga
     3241 acaacggcgc accgtatgaa ttggtgaaag aagtgaagga acttggtcgt ttgccggtgg
     3301 tgaactttgc tgcgggcggc gtggcgaccc ctgccgatgc cgcgttgatg atgcagctcg
     3361 gtgtggacgg cgtctttgtt ggctcgggta tcttcaaatc tggtgaccct gccaagcgtg
     3421 cggcagcgat cgtcaaatcg gtgacgcatt atcaagatgc ttccattctt gcggaagtga
     3481 gcaagaacct cggcgaagcc atggtcggac gcaacgtctc gcagatggca gaaggcgaaa
     3541 agatcgccgg acgcggttgg taaatttatt ttggtagggg cggggttacc ccgcccctac
     3601 attgaatgat atgaaaatcg gtgttttagc gttacaaggt gattttgcag aacatgtttc
     3661 tatgctgaag agagtcggtg tagaagctgc ggaagtacgc ctgcccaagc atttggatga
     3721 tttgaacggg ctcatcatcc ctggcggcga gtcgacgacg attggcaaat tggcagtggc
     3781 gtataacttg atcgacccgt
//
LOCUS       DAOQFT010000210         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13466_length_3799_cov_8.391560, whole genome shotgun sequence.
ACCESSION   DAOQFT010000210 DAOQFT010000000
VERSION     DAOQFT010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13466_length_3799_cov_8.391560"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="modA"
                     /locus_tag="PLR93_06475"
     CDS             <1..648
                     /gene="modA"
                     /locus_tag="PLR93_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH26647.1"
                     /translation="ENFQTANPGVTVTFNFAGSQALRTQIEEGAPADIFASASGKEMD
                     AAIEGKFIAEDSNQVFLTNKLVVILPADNPAGLETLEDLANSGVKIVLAAEEVPVGNY
                     ARQALDLMNGSFGADFKDKVLANVVSNEDNVKQVVSKVQLGEADAGIVYTSDAVAAPE
                     LKSIEIPAELNVIAKYPIAPLVKSENADLAQAFMDYVLSDEGQAILAKWGFGPKP"
     gene            697..1485
                     /locus_tag="PLR93_06480"
     CDS             697..1485
                     /locus_tag="PLR93_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26648.1"
                     /translation="MKKIPSRSKWLFILPSIFMLALFLLPLSAILIRSVGDDFFTHAF
                     SEQAFKALRLSLTTSTITTLVAIMFGTPLAYMLARWKLRFKAWLELLIDLPIVLPPSV
                     AGLALLIAFGRRGIFGSVLEAFGVTLPFTTTAVVLAQLFVAAPLYIRSARIGFAEVDD
                     QLEEAANVEGANQWQMFAAIMLPLAGRALISGAILTWTRALGEFGATILFAGNLEGVT
                     QTMPMAIYLGFERNVGVALSLSVVLIFVSTLLLLLTRKLEKEKD"
     gene            complement(1583..1804)
                     /locus_tag="PLR93_06485"
     CDS             complement(1583..1804)
                     /locus_tag="PLR93_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26649.1"
                     /translation="MAKKVWQVEKTKYCEHLQQAIAIEVEVVYPAEQLPEQTPRVMAR
                     RCSKAIECNCVEHPSCSLSGTNPDLDPVE"
     gene            complement(1915..3135)
                     /locus_tag="PLR93_06490"
     CDS             complement(1915..3135)
                     /locus_tag="PLR93_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNH26650.1"
                     /translation="MRRLFLPLLLTVLACGGNGIPGPTPTPLSPTATVSAPQTESQPP
                     ADPATLPPTAVMIESAQQFPDPTAYEWKPLMEGLDRPVDLQSAQDGTGRLFIIEKYGA
                     IRIFKDGQLSPQPFLNIDDRVNDDSNEMGLLGLAFHPNFEQNGYFYVNYTGDGGNTRI
                     SRFTANGDVADPNSEQVLMVIDQPFPNHNGGAVAFGPDGYLYLGLGDGGAAGDPFKNG
                     QNTETLLGKLLRIDVNNGDPYAIPADNPFGNEVWAYGLRNPWRISFDRATGDLWIGDV
                     GQNQYEEIDYLPAGSPGGANFGWSVMEGLHGYDGEAQPGLLLPVVEYSHEIGCSVTGG
                     YVYRGSMPEWNGVYLYGDYCSGSVSGLILVDGQWQSRVLFEAVATITSFGQDEAGELY
                     FTSDNGSIYILAKK"
     gene            complement(3136..3507)
                     /locus_tag="PLR93_06495"
     CDS             complement(3136..3507)
                     /locus_tag="PLR93_06495"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNH26651.1"
                     /translation="MTEKKSSDIIVNQQGGVVRNLVNQLKLIFRLMGDKRVSVLAKLV
                     PLGAFAYLIMPADLAPNVVLPVIGMVDDAAILWLGTYIFTELCPPEVVEEHMKALAGN
                     MNPKDVNNEDVVDGETTEVKE"
BASE COUNT          948 a          984 c          926 g          941 t
ORIGIN      
        1 gaaaacttcc aaactgccaa ccccggtgtg accgtcacct tcaacttcgc cggttcgcaa
       61 gccttacgca cccaaatcga agaaggcgcc ccagcagata tcttcgcttc cgccagtgga
      121 aaagaaatgg acgccgctat cgaaggcaaa ttcattgcgg aagactcgaa ccaggtattc
      181 ctcacaaaca aactggtcgt catcctccca gccgataacc ccgccgggtt ggaaactctc
      241 gaagacctcg ctaactctgg cgtaaagatc gttttggcag ccgaagaagt acccgtcggc
      301 aattacgctc gccaagcatt ggatctaatg aatggttcct ttggcgccga tttcaaagat
      361 aaagtgctag ccaacgtggt ttccaacgaa gataacgtca agcaagtggt atccaaagtt
      421 caattaggcg aagccgatgc cggtatcgta tacacttccg atgccgttgc ggcaccagag
      481 ttgaaatcca ttgagattcc cgctgagctg aacgttatcg ccaaatatcc cattgctcca
      541 ctggtgaaat ctgaaaacgc agacctcgcc caagccttca tggattatgt cctttccgat
      601 gaaggtcaag ccattctggc aaaatggggg tttggtccca aaccataaac aaggtactat
      661 ctagcgcgag aggaaaatcc tcccgcgctt ttgtctatga aaaaaatccc ctctcgttca
      721 aaatggctct ttatccttcc cagcattttc atgctggcat tatttctatt gccgctttcc
      781 gccattttga tccgctcggt gggagatgat ttcttcacgc acgccttttc agaacaagcc
      841 ttcaaagccc tgcgtttaag tctcaccact agcactatta ctactcttgt agcgattatg
      901 tttggcacgc cactagccta catgttggct cgttggaagt tacgcttcaa agcctggctt
      961 gaattgctga tcgaccttcc catcgtccta ccgccttcgg tggctgggct cgccctgctg
     1021 atcgccttcg gtcggcgcgg aatcttcggg tccgtgttag aggcgtttgg cgtcacctta
     1081 cctttcacaa caacggcagt cgtcttggcg caattattcg tcgccgcccc gctttatatc
     1141 cgctcggcac ggattggctt cgccgaagta gatgaccaac tcgaagaagc tgccaacgtg
     1201 gaaggcgcaa atcaatggca gatgtttgcc gcgattatgc taccgttggc ggggcgtgcc
     1261 ctcatcagtg gcgccatcct cacttggacg cgcgcgcttg gcgaatttgg ggcaaccatt
     1321 ttatttgctg ggaatttaga aggcgtcacg caaaccatgc cgatggcaat ttacctcggc
     1381 tttgaacgaa atgtaggggt tgcattatcg ctttcagtgg tgctcatttt tgtttccacc
     1441 ctattgctat tactcacaag aaaactggaa aaagaaaaag actaacccat ttaacggctt
     1501 acgccgcgcg ttgcgcggcg tagccttaag accaactcag agggaggaga gagagtttgg
     1561 atgaacagcg ttgtgcgctg ctctattcaa cagggtcaag gtcaggattt gtcccgctta
     1621 gggagcaaga ggggtgttct acgcaattgc attcgattgc ttttgaacag cgccttgcca
     1681 taacacgggg agtttgctcg ggcagttgct cagccgggta tacaacctcg acttcgatgg
     1741 cgattgcttg ctgcaagtgc tcgcaatatt tggttttttc aacttgccaa accttttttg
     1801 ccataactgc tcctgactca gatgtcccta gtttagaacc aaaacgcctg tcagaccagt
     1861 gacaggcgtc ataaactcgg atgacaaagc acctattatg gatgtgataa gtctttattt
     1921 ctttgcaagg atgtaaatac tgccgttatc gctggtgaag tacaactcgc cagcctcgtc
     1981 ttgcccaaaa gaggtgatgg ttgccaccgc ctcaaacagg acccgagact gccactgccc
     2041 atcgaccaga atcaaccctg aaacagagcc agaacaatag tcgccgtaca agtacacgcc
     2101 gttccattct ggcatggaac cacgatagac atatccgcct gtcacagagc agccaatttc
     2161 atggctgtat tcgactaccg gtaacagtag ccctggctgg gcttccccgt cgtacccatg
     2221 cagcccttcc attacactcc agccaaagtt ggcaccgcct ggcgagccgg ctggcaaata
     2281 gtcaatctct tcatattggt tctgccccac atcgccgatc cacagatcac ccgtagcgcg
     2341 gtcaaatgaa atgcgccacg ggttacgaag cccatatgcc cagacctcat tgccgaatgg
     2401 attatccgca gggatagcat atgggtcacc attgttcacg tcaatgcgca ggagttttcc
     2461 gagtagggtt tcggtgttct gcccattctt gaacgggtca cctgcagcac cgccatcgcc
     2521 cagcccgaga tagagataac cgtcgggtcc gaaggctaca gcgccgccat tgtgattcgg
     2581 gaaaggttgg tcgatgacca tcaacacctg ttcgctgttg gggtcggcaa cgtcgccatt
     2641 ggcggtaaag cgcgagatgc gggtgtttcc gccatcgcct gtgtagttga cgtagaaata
     2701 gccattctgc tcaaagtttg ggtgaaacgc caagcccagt agccccattt cattactatc
     2761 atcattgaca cggtcatcaa tgttaagaaa aggttgagga gaaagctgcc catctttgaa
     2821 gatacggatg gcaccatatt tttcgatgat gaacaagcgc ccagtgccat cctgcgcaga
     2881 ctgtaaatct acggggcgat ccaagccttc catgagaggt ttccactcgt aggcggttgg
     2941 gtcggggaat tgctgggcgc tttcaatcat caccgcggtt gggggaagag tagcagggtc
     3001 cgctggcggt tgagattcgg tttgtggtgc tgaaacagtt gccgttggag aaagaggcgt
     3061 gggggtgggt ccgggaatgc cattccctcc gcaggcaagg acggttaaca ataaagggag
     3121 gaaaagacgt cgcatttatt cttttacttc agtggtttcg ccatccacaa catcttcatt
     3181 gtttacgtct ttggggttca tatttccagc aagcgccttc atatgctctt ccacaacttc
     3241 tggcgggcac aattcggtga agatataggt accaagccag agaatggcgg catcatcgac
     3301 catgccaatt acaggcaaga cgacgttcgg cgcaagatca gcaggcataa tcaaataagc
     3361 aaaagcgccc aagggcacta actttgccaa gacactgacg cgcttatcgc ccatgagacg
     3421 aaagataagc ttcagttggt tcacaaggtt gcgcaccacc ccgccttgtt ggttgacgat
     3481 gatatcagag gattttttct cagtcatttt gttctccttt gagttgtatg ttcattatat
     3541 tcggaattgc aagacatcca ctttcgatta ttgcaatcta tatgaacata gattgatata
     3601 cgtgacaaaa gaagatgggg tgcaggtaaa aatggcagaa gcaggaaaaa aactcaaaaa
     3661 ttgggtgtaa cttattctta gcaaatacat ccaatatgta caacaataaa tttggaggat
     3721 ttccccatgg atatgattat agatttcccc ggcgggcaaa aagtagatgc ccacttccgc
     3781 ggtcacacca ttaagacag
//
LOCUS       DAOQFT010000211         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13468_length_3799_cov_4.542468, whole genome shotgun sequence.
ACCESSION   DAOQFT010000211 DAOQFT010000000
VERSION     DAOQFT010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_3799_cov_4.542468"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLR93_06500"
     CDS             <1..239
                     /locus_tag="PLR93_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26652.1"
                     /translation="IESVIYIWSKLKDRCKQEGAAIIFISSDLEEVLQYSDRLLVFFS
                     GKVSEPIDAATTSVEQLGQLIGGKGWTKKGDIHA"
     gene            232..1269
                     /locus_tag="PLR93_06505"
     CDS             232..1269
                     /locus_tag="PLR93_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26653.1"
                     /translation="MLKTKAGGFIFQLIAFLLAMGFVSLILLAVGASPMEAYKNMLTG
                     SVGSMRKFAEVLVSFVPLLLVTAGLLITFSAGLWNIGVEGQIVLGAIGSVWVLRQFVD
                     STVSPAIVMTLAILGGMVAGALWASLAGALKTFGGVNEIFGGLGLNFVSTALTLYLIF
                     GPWKREGVASMSGTEPFPDAFSLPQLPDLRISPYALVIALIAIVIVYFILQGTYFGLK
                     IKAVGKNIRAAYLLGIPTWQYSLYSFLICGALAGIAGSLQVTAVYHRLIPSISSGYGY
                     LGLMVAMLVNYQAIWALPVALFFAALNIGSIQLPIVMQLDSSLSGVLQGVLVLFVLLT
                     EGARQKFGKKG"
     gene            1277..2275
                     /locus_tag="PLR93_06510"
     CDS             1277..2275
                     /locus_tag="PLR93_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26654.1"
                     /translation="MNQETLLVGLAGVLAAAAPIIFAVMGETLSERAGVINLSMNGTI
                     LLTAMSGFAVAYKTDSLILGFLAGMLIGALVALVVAFASITLKQSQVAIGFVLALTCR
                     DLAYFLGNPFMGVTGPRLQATPIPGLSDIPILGPVFFRQEVITYVSFLAIFIAWLWIY
                     RTRPGLMLQGIGEKPAAAYVRGANVNLMRYVYTVLGGALIGLAGPTYSLGVRAGWMGT
                     ISGLDGIGWIALSITIFGGWNPARAAFGSYLFAFLQWLGLVLQPVLTQVPSQVLQVAP
                     FPLMILTLLLVNIGNAEWVERTLAAMPESARKFTAKLLRAMRASPPAALGVPFERD"
     assembly_gap    2325..2424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2509..3777
                     /gene="thrC"
                     /locus_tag="PLR93_06515"
     CDS             2509..3777
                     /gene="thrC"
                     /locus_tag="PLR93_06515"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNH26655.1"
                     /translation="MNKFNGYRCSICGSEYLPGQVTYTCPKDGGNLDVVLDVDTIKKK
                     FQPEDLISRTEASLWRYLPLLPVNEPVGDSTPLHASGWTPVFALPRLAEKLHLKHLWL
                     KDESRNPTASFKDRASALVVTRARELKSEIVVTASTGNAGAALAGMAAAVGQKAVIFA
                     PKTAPQAKVAQLLVFGAKVILVDGTYDDAFDLTVKASNEFGWYCRNTGYNPFTLEGKK
                     TAAFEIWEWWIEAHRDWHKKDSTLDNHSPLTIFVSVGDGNIISGIHKGFKDLLALGWI
                     SNMPRIIGVQAEGSAAIANAFHAGTETITPVSATTIADSISVDLPRDGVRAVRAAQQT
                     DGTYIVVKDEDIIKAIAELGKIGVFAEPAGATAYAGLVKATSQGVVGSDDPVLVLNTG
                     SGLKDVRAALQAVQSAPIIEPTLEAVKKIL"
BASE COUNT          812 a          822 c          982 g         1083 t
ORIGIN      
        1 atattgagtc ggtgatctat atttggagca aactcaaaga tcgctgcaaa caagagggcg
       61 cagccatcat ctttatttcg tctgacttgg aagaagtttt acagtacagt gaccgtttgc
      121 tagtgttctt tagcggtaag gtttcagaac cgatcgatgc tgcgacaacc agtgtggaac
      181 aactaggtca attgatcggt ggtaagggct ggacgaaaaa aggagatatc catgcttaag
      241 accaaagcgg gtgggtttat ttttcaatta atcgcgttcc tgcttgctat gggatttgtc
      301 tcgttaattc tgttggcagt aggtgcttca cctatggaag cgtataaaaa tatgctgacc
      361 ggctcggttg gctctatgcg taaattcgcc gaagtgttgg tgtcatttgt gccattgctt
      421 ctggtaacgg ctggtttgct tattaccttc tctgccgggc tttggaatat cggtgtggaa
      481 ggtcagattg ttttaggtgc cattggttcg gtttgggtct tgcgccaatt cgtagatagt
      541 acggtttctc ctgctattgt gatgactctg gcgattttag gcggaatggt tgctggtgcc
      601 ttgtgggcat cgttggcggg cgctttgaaa acctttggcg gtgtgaatga aatttttggt
      661 ggcttggggc tgaactttgt ctccacggca ttgaccttgt acctgatctt cggtccatgg
      721 aagcgtgaag gtgtggcgtc catgagcgga accgaaccgt tccctgatgc attctctttg
      781 ccacaattgc cagatttgcg tattagcccg tatgctttag tgattgcatt gatcgcaata
      841 gttattgtgt atttcatatt gcaaggcacc tattttggct tgaaaatcaa agctgtgggc
      901 aagaacatcc gcgctgcgta tttactcggt attcctacct ggcaatattc tttatattct
      961 ttcctcatct gtggagcctt ggcgggcatc gctggttctt tgcaagtgac tgcggtgtat
     1021 catcgtctga ttccttccat ttctagcgga tacgggtatc ttggcttgat ggtggctatg
     1081 cttgttaatt accaagccat ctgggcattg ccagtcgctc ttttctttgc ggcgttgaac
     1141 attggtagca ttcaattgcc aattgtgatg caattggatt cgtctctctc aggtgtgctg
     1201 cagggtgttt tggtgctctt cgttttgctc actgaagggg ctcgccaaaa atttgggaag
     1261 aagggataag gcatccatga atcaagagac tcttcttgtt ggcttggcgg gcgtacttgc
     1321 tgcagcggct cccattattt ttgcagtaat gggcgagacc ctttctgaac gagctggcgt
     1381 catcaattta tccatgaacg gaaccattct gttgactgct atgagcgggt ttgcagtcgc
     1441 ttataaaaca gatagcttga ttcttggttt tcttgctggc atgttgatcg gtgcgcttgt
     1501 tgctttagtg gttgcatttg ccagcattac cctcaaacaa tctcaagtag caattggctt
     1561 tgttttggcg ctcacatgcc gcgacttggc atactttctc ggcaacccat ttatgggtgt
     1621 tacaggcccg cgtttgcaag cgacacccat tcctgggctg agcgacattc cgattctcgg
     1681 tcctgtattt ttccggcagg aagtgattac ctatgtcagc ttcttagcga tcttcattgc
     1741 ctggttatgg atttatcgca cgcgccccgg tctaatgcta caaggcattg gcgaaaaacc
     1801 agcagccgcc tatgtgcgtg gcgcgaatgt gaatctaatg cgctatgttt atacagttct
     1861 gggcggtgct ttgatcggtt tagcaggacc tacatattcg ttgggcgtcc gcgctggatg
     1921 gatgggaacc atttccggct tagatggaat cgggtggatc gccctttcca tcacgatctt
     1981 tggtggttgg aatccggctc gcgctgcgtt tggttcatat ctcttcgctt tcttgcaatg
     2041 gcttgggctg gttttgcaac cggtgcttac ccaagtccct tcgcaagtct tgcaggttgc
     2101 gcctttcccg ttgatgatcc tcaccctctt gctcgtcaat ataggcaatg cggaatgggt
     2161 tgagcgcaca ttggcagcca tgccagaatc tgcgcgaaag tttacggcga agttattacg
     2221 agccatgcgt gcctcgccgc cagctgcttt gggcgtccca ttcgagcgtg attagttgca
     2281 acataaaagg actctcgtat gagggtcctt tttgattttt atatnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnngattag ttgcaacata aaaggactct cgtatgaggg
     2461 tcctttttga tttttatatc gggtaaaatt aagccctaga ggaaatgtat gaataaattc
     2521 aacgggtatc gttgttcaat ttgtgggagt gagtatcttc cagggcaggt cacatatacg
     2581 tgtccaaaag atggcgggaa cttggatgtg gtcttagacg ttgataccat caagaagaaa
     2641 tttcagcctg aagatctcat ctctcgaacg gaggcttcgc tgtggaggta tttaccgctg
     2701 ttgccggtca atgaacccgt aggcgattcc acgcctcttc acgcctccgg ctggaccccg
     2761 gtctttgccc tgccacgatt ggcagaaaaa cttcacctga agcatctctg gctcaaagat
     2821 gagtcgcgca accctaccgc ctcgttcaaa gatagagcta gcgccttggt cgtgacgcgt
     2881 gcccgcgagc tcaaatcgga gatcgtagtc actgcttcaa cgggcaacgc cggagcagca
     2941 ttagcaggca tggctgccgc tgttggacaa aaagccgtta tctttgcgcc caaaactgct
     3001 ccacaagcga aggttgcgca attgctggtc tttggtgcaa aagtcattct ggtagatgga
     3061 acctatgatg acgcctttga cctgaccgtt aaagcctcaa atgaatttgg ttggtactgt
     3121 cgtaatacag gctataaccc gttcacactg gaaggtaaga agaccgccgc ttttgagatc
     3181 tgggaatggt ggattgaagc tcatcgtgat tggcacaaga aagatagtac cttggataat
     3241 cattctccgt taactatctt tgtctctgta ggggacggga atattatctc tggcattcac
     3301 aaaggcttca aagatttatt ggcactcggc tggatttcaa atatgcctcg cattattggt
     3361 gtgcaagccg aagggtctgc agcaattgct aatgccttcc atgcagggac agaaaccatc
     3421 acacccgtgt ctgccacaac cattgcagat agcatctcgg tcgatttgcc gcgagacggt
     3481 gtccgtgcag tgcgggcagc tcagcagacc gatggcacgt atattgttgt caaagatgag
     3541 gatattatca aagccattgc agagctggga aagatcggcg tctttgccga acctgctggg
     3601 gcgacggcat atgccggttt ggtcaaagcc acaagccagg gcgtggttgg aagcgatgat
     3661 cctgttctcg tcctgaatac aggcagtgga ctcaaagatg tgcgagcggc attgcaggca
     3721 gtgcagtctg cgccgattat tgagccaact ttagaagcgg tgaaaaagat tttatgaaaa
     3781 aaactaacga tcagtggag
//
LOCUS       DAOQFT010000212         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13488_length_3795_cov_7.125668, whole genome shotgun sequence.
ACCESSION   DAOQFT010000212 DAOQFT010000000
VERSION     DAOQFT010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13488_length_3795_cov_7.125668"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PLR93_06520"
     CDS             complement(<1..852)
                     /locus_tag="PLR93_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26656.1"
                     /translation="MLNIDEEVKKVLNEVGPGRMSSTAYDTAWVARLGEIDKDLSDQA
                     VNWLLENQLPDGSWGASDVFYYHDRVISTLIAMIALKEHGGNSHHQAQVEKGVEALER
                     ITSDGEHSFASDLNGATVAFEMVAPILLAEAKELGIIKQQGERILGRLSNMRQEKMRK
                     LAGYKINRYVTPAFSAEMAGIDGQHMLEVENLQEANGSIGHSPSATAYFALRVKPGDA
                     KAIDYIKSSVAKNGGAPDLYPFDVYERAWALWNLSLTDYFNGDTRSFADPILKYLAAA
                     WKPGQGIG"
     gene            complement(861..1775)
                     /locus_tag="PLR93_06525"
     CDS             complement(861..1775)
                     /locus_tag="PLR93_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26657.1"
                     /translation="MSPMSLEAFLALPTEELANLVRGDRVKTCVFPFNGTRRWFLLER
                     PSSAGGDLAKQYIELTTQGYINTYKLVFDHGIDNIVAPAFGGELLVNRGEEYMRQIGT
                     SLSLLAIHPDFVNFYKEYDVRVHFYGEYRKQFKDTSYAYIIDQFDEVTRQTADHTSRA
                     LFYGVFAEDATMTTAELAIQHVQQTGTHPTRRDLIERYYGEFIEKADIFIGYEKFNVF
                     DYPMLGLGEESLYFTVAPSLYMNDRQLRNILYDFLYLRPVQDPDYFSMPQADFEAMRR
                     YYEANREVTFGIGEMRGGIWYATPHLKK"
     gene            complement(1779..2651)
                     /locus_tag="PLR93_06530"
     CDS             complement(1779..2651)
                     /locus_tag="PLR93_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNH26658.1"
                     /translation="MKRIIVAGIYRSGTSLITELVHRWGAYAGREEDIFKDEFGYMEH
                     FALQKLNDELLNNNSRVPTPVETLLEKAEDAGLRERALSILAEMDEEAQANHAAAWVW
                     KDPRLPLVLPFWADVWGDVIYVIPVRHPVETIFSAAKMDGLDPEQLPLSAGLAYWQFC
                     MLNLLQYTANTPRKLFMAYDQLTKDPQRECERLCRFLDAQCEVDSDPSDRIRSMTEQV
                     AAGERHFNHPRSLAEVETSTREQRALYDFLRVKTLYPDEPFHKDDFAMYPGWFEYLSA
                     MDMLFSAGDAGQEA"
     gene            3191..>3795
                     /locus_tag="PLR93_06535"
     CDS             3191..>3795
                     /locus_tag="PLR93_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH26659.1"
                     /translation="MTLLDQFNKWKETTLKKSLDKFKERKGRFETSAGIEVPRLAIPD
                     GNEERGDLAPTVADENYVNKLGFPGEFPYTRGVQPTMYRSRFWTMRQYAGFSTAEESN
                     KRYRYLLEQGQTGLSVAFDLPTQIGYDADDPIAQGEVGKVGVSISSIKDMEQLFDQIP
                     LDKVSTSMTINAPAGVLLAMYIAVAKRQGADMRELRGTIQN"
BASE COUNT          986 a          912 c          929 g          968 t
ORIGIN      
        1 accaatcccc tgccccggtt tccatgctgc tgcaaggtat ttcaaaatag ggtcggcaaa
       61 agaccgggta tcgccgttga aatagtcagt taaagataaa ttccataagg cccatgcccg
      121 ttcatacaca tcaaacggat agagatcagg cgcgccgccg tttttcgcca cagagctctt
      181 tatataatct atcgcttttg catctccagg ttttacccgc aaagcaaaat aagctgtggc
      241 agatgggcta tgcccaatgg aaccattcgc ttcctgtaag ttctcaacct caagcatatg
      301 ttgaccatct ataccagcca tctcagccga aaaggctgga gtgacatagc ggttgatctt
      361 atatccggca agtttgcgca tcttctcttg ccgcatgttg ctcaggcgac ctagaattcg
      421 ctcgccctgt tgcttgataa tgcctagctc cttggcttct gcaagcaaga tcggagcgac
      481 catttcaaaa gcaactgtgg caccgtttag gtctgaagcg aagctgtgct ctccatcaga
      541 agtgatacgc tctaatgctt ctacaccctt ttcaacttga gcttgatgat gagaattccc
      601 gccatgttcc ttaagcgcaa tcatggcaat cagcgtgcta atgacacggt cgtgataata
      661 gaacacgtcg ctggcgcccc aactaccgtc tggcaattga ttctcaagca gccagttgac
      721 agcttgatcg cttaagtctt tatcaatttc gcctaagcgt gcgacccaag cggtgtcgta
      781 ggcagtgcta ctcattcgtc cgggtcccac ctcgttcagt acttttttta cttcttcgtc
      841 gatgttaagc atgatgaact ctacttttta aggtgtgggg tggcgtacca aattcctccg
      901 cgcatctcac ctattccaaa ggtgacctcg cggttcgctt cataatatct gcgcatggct
      961 tcaaaatctg cttggggcat actgaaataa tcagggtctt gcaccggacg cagataaaga
     1021 aaatcataga gaatatttct caactgacgg tcgttcatat atagagaagg agccaccgta
     1081 aagtacaagc tctcttctcc caagccaagc atgggatagt cgaagacgtt gaatttttcg
     1141 tagccgatga aaatatccgc tttttcaatg aattcgccgt aataacgctc gatcaggtcg
     1201 cgccgggtgg ggtgcgtacc cgtttgttgg acgtgttgaa tggcaagctc tgcggttgtc
     1261 atggtggcat cttcggcaaa gaccccatag aacaaagcgc gagacgtatg atctgctgtc
     1321 tgccgtgtga cctcatcgaa ttgatcaatg atgtaggcat aagaagtatc cttaaactgc
     1381 ttgcgatact ctccataaaa gtggacgcgt acatcatatt ctttatagaa gttgacaaag
     1441 tctgggtgaa ttgccagcaa agacaggctg gttccgatct ggcgcatgta ttcttcaccg
     1501 cggttgacca ataactcgcc gccaaaagcc ggggcgacga tgttatctat gccgtggtca
     1561 aagaccaatt tataggtatt gatatatcct tgcgtggtca actcgatgta ttgtttggca
     1621 agatcgccgc cagcagatga tggacgctct aacaaaaacc agcggcgcgt gccattaaaa
     1681 ggaaagacac aggtttttac cctatcgccg cggacaaggt ttgccaactc ttcggtgggc
     1741 agggcaagaa aagcttcaag agacatagga gacatttctt atgcctcctg cccagcatcc
     1801 ccggcagaga agagcatatc catggcagag agatactcaa accaaccggg gtacatggcg
     1861 aaatcgtctt tgtggaaagg ctcatcgggg taaagggttt tgacgcgcaa gaaatcatat
     1921 aacgcccgtt gctcacgggt ggatgtttcc acttctgcca gagagcgtgg atggttaaag
     1981 tggcgctccc ccgctgcgac ctgttcggtc atggaacgaa tacggtccga tgggtcgcta
     2041 tcgacttcac attgcgcatc gagaaaacgg cacaggcgtt cacattcacg ctgtgggtct
     2101 ttcgtaagtt ggtcgtacgc catgaatagt ttgcgagggg tattggctgt gtattggagc
     2161 aagttcaaca tgcaaaactg ccagtacgcc aagccagccg aaagcggaag ttgctccggg
     2221 tcgagcccat ccattttggc ggcggagaaa atcgtctcta ctgggtgacg cacgggaatt
     2281 acatagataa catcacccca tacatctgcc caaaaaggaa gcaccaaagg caagcgtggg
     2341 tctttccaaa cccatgccgc ggcatgattc gcctgcgcct cttcatccat ttcagctaag
     2401 atgctcaagg cacgttctcg caacccggca tcttctgctt tttcaagcaa ggtctctaca
     2461 ggtgtgggaa cgcggctgtt attgtttaga agttcatcgt tcaatttctg taaggcgaag
     2521 tgctccatat agccaaattc gtctttgaaa atatcttctt cccgccctgc atacgccccc
     2581 caacgatgga ccaactctgt gattagtgat gtcccacttc gataaattcc ggcaacgatg
     2641 atacgcttca ttgtcatttg tccttttatg tatcaaaaag tataaagtta ataactcttc
     2701 tacggtatga acaaaattaa gaacgctgat ctcttcagga attcatatac aacaatcccc
     2761 catctttagg cacaacccat aggctcaaat gcaaaatgtt tcggcgaagg gatggctctc
     2821 agaaaggctt gcccaagcat gggcggagta agaagcggct ctttttggat tttgtgcgcc
     2881 cggtcaattt cccctctgga tatgttatta aatagaaggg cttacagtct tgcatcacac
     2941 gtatatgcag gagtgccgtt gaagggcggt taagcaacgt tgtatagaac cttgattcaa
     3001 tgcttgttac attaattatt ttacaggtat atacgacttt agaatttaac aaaaatgcaa
     3061 gaaacatgtt aaattttggt aatgtagaaa aaaggaggtt tttcgcggca gggggtgcta
     3121 cttatcctac aagttattcg gggttcgctt tatacttgag gggcagtcac cacgaaaaca
     3181 aaggtgaaac atgactttat tagaccagtt caacaaatgg aaagaaacta cgctcaagaa
     3241 gtctttggat aaattcaaag aacgtaaggg acgttttgaa accagcgccg ggatcgaagt
     3301 gccgcgcctc gcgattcccg atggaaatga agagcgaggg gacctcgccc ctacggtggc
     3361 ggatgaaaat tatgtaaaca aacttggttt ccccggagaa tttccctaca cccgcggcgt
     3421 acagccgacg atgtaccgtt cacgtttttg gacgatgcgc cagtatgcgg gcttttcgac
     3481 ggcggaagaa tcgaacaagc ggtatcgtta tttgctcgaa caaggtcaaa cggggctgtc
     3541 ggttgcgttc gacctcccca ctcaaattgg gtatgatgcg gatgatccca tcgcgcaggg
     3601 agaggtcggt aaggtgggcg tgtccatttc gtcgatcaaa gacatggagc agttgttcga
     3661 tcagatccca ttagataagg tttcgacttc gatgacgatc aacgctccgg cgggcgtgtt
     3721 gctggcgatg tatattgcgg ttgccaaacg ccagggcgcg gacatgcgcg aactgcgcgg
     3781 gacgatccag aacga
//
LOCUS       DAOQFT010000213         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13644_length_3766_cov_7.368903, whole genome shotgun sequence.
ACCESSION   DAOQFT010000213 DAOQFT010000000
VERSION     DAOQFT010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13644_length_3766_cov_7.368903"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLR93_06540"
     CDS             <1..549
                     /locus_tag="PLR93_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNH26660.1"
                     /translation="GVHRVQRVPATESAGRIHTSTATVTVMAEVEEVDVVIPETDIEI
                     EVYRSSGAGGQNVQKNSTAVRLYHKPTGMIVTCQDERSQLQNKLRALSILRARLFEIE
                     EAKRQAELEADRRSQVGSGDRSEKIRTYNYPQSRVTDHRIGLSNYNLAGVMDGDIEGF
                     IDELTTRDEAGKLASTGVEDDE"
     gene            646..2757
                     /locus_tag="PLR93_06545"
     CDS             646..2757
                     /locus_tag="PLR93_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH26661.1"
                     /translation="MKNYQLPLRILSIVVVLIIAGMTRARAVQHLNIDFDEDDYTRAA
                     QEFAHLIRTSNWSGFLETNYRPEHPPLAKIVMGISLLTTPEKDLIADRPTSAEPNKYL
                     PRVILKPERTVNAVFGWLTVALLAIVNPLAGLSLATHSITVKYNSQAMLEALPALTSF
                     CMVLAYLQFKKNHNTRWLITSAVFLGLTAASKYLYCVIALAILADWFLTSKEQEELKS
                     FFKQALIWGTLGILIFFVTNPFLWANPIQRLRESVLYHAGYSSSSEEVKDAGFPVWQP
                     VQWLVTSAADWEEDAFFTGPDLLIAILAAFGFARLWKRERLYVLWFGFALLFLLLWST
                     KWPQYILILTVPLSLAAAEGLTQIWQNILEGLKAPKTVYNKKESRQAIPWLVPGLIVF
                     TVLTLLPFFFQLGVSVTDFSSQSIRDGFQGGIWREVSGGLTGQITPTSPEVAYNSNKV
                     QYIGFQAYQPVFEYIVSVNKIFVFNILWMVVSTSIQVLLGLGLAILLNQRGVKLGKFW
                     QTLFILPWAIPEFIGALMWLNIFVPEIGWLSLAAKKYGDAFPLQSLIGWQSTPSLWFL
                     VLLIPAIWYGTPLIMLASNASLKTIPKEIFEAASLDGANSWQILQHMVLPLIRPVLLP
                     AIIIRGIYAFNQFYLFQAMPFSEGTLANFSYNVFNPSSRFGGGQFAISAVINIITVLI
                     LIVLVSTFNRRMQTREEFINA"
     gene            2750..3601
                     /locus_tag="PLR93_06550"
     CDS             2750..3601
                     /locus_tag="PLR93_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNH26662.1"
                     /translation="MRKISLPRQIATQLIFLLVGLFILIPVWSTFRLAFDGSLRGRPT
                     EFTWLPKEFSTEAFTKVLDRPYQSVEFNILLRNSMIVSIGAAVLAVILGITLAYGFAR
                     FRFPAKQTGLFVLLLAALLPPIAFATPLYIILSLLKIRTTLLGLVIVYAAFAMPFAIW
                     NMRATFAAIPKELEEAAYLDGANHYQTFLNISLPISLPSISVTAIIAFLMGYTEFAIG
                     WLFVDKADNVTLSMAVYALVNGQFSGAQPWSYLGSLAIIMSIPVMVIFIFFQETLMER
                     LLFQETS"
BASE COUNT          897 a         1028 c          805 g         1036 t
ORIGIN      
        1 ggtgtgcatc gcgtacagcg cgtccccgca actgaatccg ctgggcggat tcatacctct
       61 accgccaccg tcaccgttat ggcggaagtc gaagaagtgg atgtcgtcat ccctgaaacc
      121 gatatcgaga tcgaagtcta tcgttcgtct ggtgcgggtg gtcagaacgt gcaaaagaac
      181 tccactgccg tgcgcttata tcacaaaccc actggcatga tcgtcacctg tcaggatgaa
      241 cgctcacagt tacaaaacaa actgcgtgcg ctttcgatct tgcgcgctcg tttgtttgaa
      301 attgaagaag ccaaacgcca agccgaactc gaagcagacc gtcgctcgca agtcggctcg
      361 ggcgatcgct ccgaaaaaat ccgtacctac aactatccac aaagccgcgt tacagatcac
      421 cgcatcgggt tatcgaatta caacctcgcc ggtgtgatgg acggtgacat cgaaggcttc
      481 atcgacgaac tcaccacacg tgatgaagct gggaagctcg catccactgg cgtcgaagac
      541 gacgaataaa attcgcacaa caggctggag aactccagcc tgttttcaat aatccactct
      601 acggtattac aattcaggcg gaagaagagg atacaatcca tcgccatgaa gaactaccaa
      661 ctcccccttc gcatcttgag cattgttgta gtccttatca ttgctgggat gactcgcgcc
      721 cgcgcagttc aacacctcaa catagacttt gacgaagacg attacacccg cgccgcccaa
      781 gaattcgcgc atctcatccg gacctcaaac tggtctggct ttctggaaac gaactatcgc
      841 cctgagcacc ccccgctggc aaagatcgtc atgggcatta gcctgctcac taccccagaa
      901 aaagacctga tcgcagatcg ccccacttca gctgaaccca ataaatattt gccgcgtgtc
      961 atcctcaagc ccgagcggac ggtcaacgcc gtcttcggct ggctaaccgt ggcattgttg
     1021 gcaatcgtca atcctctggc tgggttgagt ctcgctacgc atagcatcac cgtcaaatac
     1081 aactcgcaag ccatgctaga agctctgcct gcattgacca gtttctgcat ggtattggcg
     1141 tacttacaat tcaaaaagaa tcacaatacc cgctggttaa ttacatcggc cgtctttctt
     1201 ggtcttactg ccgcctcaaa gtatctttat tgtgtcattg ccctcgctat tctcgcggat
     1261 tggtttctga catcgaaaga acaagaagag ctcaaatcat tcttcaagca agccttgatc
     1321 tggggcacgt tagggattct catcttcttc gtgacgaatc cattcctctg ggcaaatcca
     1381 attcaaagac tgcgtgaatc ggttctttat catgctggct attcttcaag ttcagaagaa
     1441 gtcaaagatg ctgggtttcc cgtttggcag cctgtccaat ggttggtaac cagcgccgca
     1501 gactgggaag aagatgcttt ctttacagga ccagatcttc tgattgcaat ccttgccgca
     1561 tttgggtttg cacgattgtg gaagagagaa cgtttgtatg tattgtggtt cggctttgcc
     1621 ttgttgtttt tgcttttatg gtctaccaag tggccgcaat acattctgat actaactgtc
     1681 ccgctttcgc tcgccgccgc agaaggactc acacaaatat ggcaaaacat tcttgaaggg
     1741 ctcaaagccc caaaaactgt gtacaacaaa aaagaatcac gccaagctat tccctggctt
     1801 gtgccgggct tgattgtgtt tactgtttta accctgcttc cgtttttctt tcaacttggc
     1861 gtttcagtta cagactttag cagccagtcc atccgtgatg gattccaagg cggcatctgg
     1921 cgcgaagtaa gtggtgggct tacaggacaa attacaccaa catctcctga agttgcttac
     1981 aattcaaata aagttcaata tattggtttc caagcttatc aacctgtatt cgagtatata
     2041 gtttcagtaa ataaaatttt tgttttcaac atcctttgga tggtggtttc tacgagtatc
     2101 caagtccttt tagggttagg gcttgctatt ttgcttaacc aacgcggggt aaaacttggc
     2161 aagttctggc agactctttt cattctgcct tgggcaattc ctgaatttat tggcgctttg
     2221 atgtggctga acatctttgt gccagaaatt gggtggttaa gccttgccgc caaaaagtat
     2281 ggcgatgcct ttccactgca gtctctaatt ggttggcaaa gcacacccag tctttggttc
     2341 ttggttttgc ttattcctgc catatggtac ggaacacctt tgattatgct cgcttcaaat
     2401 gcgagcctca aaaccatacc gaaagaaatc tttgaagccg cctctcttga tggagccaat
     2461 agttggcaaa tcttacagca tatggtcttg ccgttgataa gacctgtttt gttacctgcc
     2521 attatcatac gcggtattta tgccttcaat cagttctatc tatttcaagc tatgccattc
     2581 agcgaaggta ctctggcaaa cttttcttac aatgtattca atccttccag cagatttggc
     2641 ggaggtcaat ttgcaatctc tgccgttatc aacatcatca cggtcctaat cctgatcgtc
     2701 ttggtcagca ccttcaatcg ccgcatgcaa actcgcgagg agtttatcaa tgcgtaaaat
     2761 atccctgcct cgtcagatcg caacccaact catcttcttg ttggtcggtc tcttcatcct
     2821 catcccggtc tggagcacct tccgtctcgc cttcgatggc tccctgcgcg gacgtcctac
     2881 cgagtttaca tggctgccca aagaattctc cactgaagcc ttcacgaaag tattagatcg
     2941 cccctatcaa tccgtagagt tcaacatcct acttcgcaac agtatgatcg tttcgattgg
     3001 tgcggcagtc cttgcagtga tattgggcat cactctcgcc tatgggtttg cacgttttcg
     3061 ctttcctgcc aagcagaccg gcttgttcgt tttattgctg gcggcattgt tgccccccat
     3121 tgcatttgcc actccgttgt atatcatctt aagtctgctg aagattcgca caactttgct
     3181 cggcttggtg atcgtctatg cggcgttcgc catgcccttt gccatttgga acatgcgtgc
     3241 cactttcgcc gccattccaa aagaattaga agaagccgcc tacctcgacg gagcgaatca
     3301 ctaccaaacc ttcctcaaca tcagcctacc catctcgctg ccgtctattt ctgttaccgc
     3361 cataatcgcc tttctcatgg gatataccga attcgccatt ggctggctct ttgtagataa
     3421 agccgataac gtcacacttt ccatggcagt ctacgccctc gtcaacggac aattctctgg
     3481 cgcacaacca tggagctatc ttggctcact cgccatcatc atgtccattc cagtgatggt
     3541 cattttcatc tttttccaag aaaccctcat ggaacgccta ctctttcagg aaacctcatg
     3601 acccaaactc tgggcgatta tctcattcac aaaattccaa aactgccaga tgacctgacc
     3661 caacaagatg cgcaactctt gcttgcgcat atgatcggac gttcacgcac atggctgctc
     3721 gcccatctcg acgcgcctct ttccgcgccg atacttgacg cagcga
//
LOCUS       DAOQFT010000214         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13815_length_3738_cov_6.267173, whole genome shotgun sequence.
ACCESSION   DAOQFT010000214 DAOQFT010000000
VERSION     DAOQFT010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13815_length_3738_cov_6.267173"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLR93_06555"
     CDS             complement(<1..743)
                     /locus_tag="PLR93_06555"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNH26663.1"
                     /translation="MKYSLNAREGDLAQLVGLTHKHFIFMLQSGGDFQSHRGVIKHDD
                     LIGKPWGSQIFSHTGAPFFLLQPSMSDILNELPRNTQILYPKDIGYILIQMGISEGQT
                     VLEAGTGSGSMTISMATAVGPNGRIVSYEKTPDTQNLAKKNLQRIGLASRVDFKLRDI
                     QEGFDETDADAFFLDVQNPFDYIQQVRATLKPGGFFGTLVPTYNQVEKVLYALKKHNF
                     AFVEVCELMLRYYKTNPARLRPTDRMVAHT"
     gene            complement(771..2039)
                     /locus_tag="PLR93_06560"
     CDS             complement(771..2039)
                     /locus_tag="PLR93_06560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNH26664.1"
                     /translation="MQTKKIHWASLVILLILATSITFLLLIVLSLGVASLVDLFSSSA
                     EAASEMIGAVAFGFISILLLISGWFILQKTRGLEQADQNFKFPFAPWMWLVLPALVIF
                     SILFGGLITFAEIPWLNWLLLPFLTVLVIVPPIWLLFGIASNGLNLGPRWRFFSILGL
                     GMTAGPIIMIVLEMVLLLTLIIAGVAYIAITRPEMVSEIESLANMLNQGLSEDAMLNL
                     LSPYLTNPTLLAIGIGYIALLVPLIEELFKPLAVWLFARQLESPAQGFALGVLSGAAF
                     ALVESLNASADGTTSWAVIVAARTGTSVLHMLTTGLVGWGIAMAFKEKRFGRLVAAYL
                     SAVLIHGIWNAAAAGTGISALGESVGKPEWLFNYAPALVCGLLVLGIGTTSLLVASNR
                     KLRKQLELAPAVESSVESTQVQETPETPSE"
     gene            complement(2039..3271)
                     /locus_tag="PLR93_06565"
     CDS             complement(2039..3271)
                     /locus_tag="PLR93_06565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH26665.1"
                     /translation="MKRILFGLVFFGITLFFTACSTENTPTPYIVLDPVIPPTPTPQY
                     TCTVISAMPTLSPNEQSLAPAITAADFSFGPSDAPVTILEYCDFQSDGCLAMAQTIAE
                     LMRNYENQIRFVFRPLPLSTVLDKSDLSALAAFAAGEQGKFWEFYDLLFSKHGEWKNL
                     SPGEFKGWLTRETPAIGIDASQLTAAMEAEGALTQLKSAQEAMTQLYITAVPVILING
                     SLQQSYVLDYRNMYDTVGLILLGEKQFTECPPYTVDASKQYIATIETAKGNIVIQLFP
                     DKAPLAVNSFIFLAKQGWYDGVTFHRVIPGFAAQAGDPSGSGRGNPGYFFNNEPNDLV
                     FDKPGLVGMANSGPDTNGSQFFITFAPAEHLNGAYTIFGQVISGLDVAEQLTARDPSQ
                     GGFLPAGDLILHITIEEK"
     gene            complement(3274..>3738)
                     /locus_tag="PLR93_06570"
     CDS             complement(3274..>3738)
                     /locus_tag="PLR93_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26666.1"
                     /translation="ASIAGDEIGAIVIEKFYPELKPAASFPNLSLISLSFDRPDPETL
                     ERPPFDFRAQMHETRVTADALLAAGKIDEAEAYMEARRQIFLQNGYLLRKINQAYFAF
                     HGAYADVPGGAAGEDPVGPAVRALREQSPSLKDFINTIAWMWSFEQLQETIK"
BASE COUNT          983 a          894 c          979 g          882 t
ORIGIN      
        1 gtgtgcgcca ccatacggtc tgtgggacgt aagcgggcag ggttggtctt gtaatagcgt
       61 agcatcagtt cacagacttc cacaaaagcg aagttatgtt ttttcagggc atacaatact
      121 ttttcgacct gattataggt aggaacgagc gttccaaaga atccgcccgg cttgagcgtg
      181 gcgcggacct gttgaatgta gtcgaaagga ttttgcacgt cgaggaagaa ggcatcggca
      241 tccgtttcgt cgaagccctc ttggatgtca cgcagcttga agtccacgcg agaggcgaga
      301 ccgatccgtt gtagattttt cttcgccagg ttttgcgtat ctggcgtttt ctcatacgaa
      361 acgattcttc cgtttggacc aactgctgtt gccatggata tcgtcatcga gccagaacct
      421 gtgccagctt cgagtacggt ttgcccttca gagatgccca tttggatgag gatataaccg
      481 atgtctttgg ggtacagaat ttgcgtattg cgcggcaatt cattgagaat gtcggacatg
      541 gaaggctgaa gcaggaagaa gggcgctcct gtatggctga agatctgcga gccccacggt
      601 ttgccaatta gatcatcatg cttgatcaca ccacggtggc tttgaaagtc gccacctgat
      661 tgcagcataa agatgaagtg tttgtgggtt agccccacca attgcgcgag gtcgccctca
      721 cgcgcgttaa gagaatattt catgcagcga attctacctt tactttcgat ttattcagaa
      781 ggggtttctg gggtttcctg aacctgcgtg gattcaacag agctttccac agcgggagcg
      841 agttcgagct gttttcttaa tttccggttg gaggcaacca gcaaagaagt tgtaccaatg
      901 cccaacacca gtaagccgca aacgagcgca ggcgcataat tgaaaagcca ttcaggtttg
      961 ccgactgact cacccaaggc agaaatgcct gtgccagcgg cagcggcatt ccaaatgccg
     1021 tggattaaca cagcagagag atatgcggcg accaaacgtc caaagcgttt ttctttgaac
     1081 gccattgcaa ttccccagcc aaccaagccc gtggtgagca tgtgcagtac actcgtccca
     1141 gtgcgtgctg ccacaatgac cgcccaacta gtggtgccat ccgcactggc attcaagctt
     1201 tctactaaag caaacgccgc accgctgagt acacccagag caaacccttg cgccggagat
     1261 tcaagctgac gggcaaacag ccaaactgcc aacggcttga ataattcttc aatcagcgga
     1321 accagaagcg cgatataccc gatcccaatg gcgagcaaag ttgggttggt aaggtaaggc
     1381 gataacagat tgagcatggc atcttcgctt aaaccttggt tcaacatgtt tgccaaagac
     1441 tcaatttcgc ttaccatctc agggcgtgtg atggcaatat aggcaacccc ggcaatgatg
     1501 agggtcagta gaagcaccat ttcgagcaca atcataatga tcggccccgc cgtcatgcct
     1561 agcccgagaa tactgaaaaa ccgccaacgc ggaccgaggt tcagcccgtt cgaggcgatg
     1621 ccgaacaaca gccagatcgg tgggacaatg accaagacag ttagaaacgg aagcaggagc
     1681 caatttagcc atggaatttc tgcgaaggtg atcaacccgc caaataaaat gctaaagatc
     1741 accaaggcgg gtagcaccag ccacatccac ggggcaaagg gaaatttgaa attctggtct
     1801 gcctgctcca atccgcgagt cttttgcaaa atgaaccatc cgctgatgag tagcaagatg
     1861 ctgataaacc cgaatgccac tgcaccgatc atttcagaag cggcttcagc agaactgctg
     1921 aataggtcca cgagggatgc gacaccaagc gacagaacga tcaaaagcaa aaatgtgatg
     1981 ctagtcgcaa ggataagcaa tataacaaga gatgcccaat ggattttttt tgtttgcatt
     2041 atttctcttc aatcgtaata tgtaagatca aatcccctgc gggcaaaaag ccaccttgcg
     2101 aagggtcgcg cgctgttaac tgttcggcta catccaatcc actaatcact tgcccaaaga
     2161 tggtgtaagc cccattcaaa tgttcagcgg gcgcaaaagt aataaagaat tggctgccat
     2221 tggtatcagg tccagaattt gccatgccca ccaaaccagg tttatcgaac acaagatcat
     2281 tcggctcatt gttgaagaaa tagccggggt tgcctctacc gcttccactt gggtcaccgg
     2341 cttgagccgc aaagccaggg atcacgcggt gaaatgtcac accgtcgtac cagccttgtt
     2401 tcgcaagaaa gataaatgaa ttaaccgcta acggggcttt atctggaaat aattgaatca
     2461 caatatttcc tttggcagtt tcaattgtgg caatgtattg tttcgatgca tctaccgtat
     2521 aaggtgggca ttcggtaaac tgtttctcgc caagcaaaat tagccctacg gtatcgtaca
     2581 tattgcgata atccaaaaca taggattgct gtaacgaccc attgatgagg atgactggaa
     2641 ccgcggtgat gtataactga gtcatcgcct cttgcgccga tttgagctgg gtcaatgcgc
     2701 cttcggcttc catcgccgct gtaagctgac tcgcatcaat gccaatagcg ggagtttctc
     2761 ttgtgagcca gcctttgaat tcgcccgggc ttagattttt ccactcgcca tgttttgaaa
     2821 ataatagatc ataaaattcc caaaacttgc cttgttctcc agcggcaaag gctgccagtg
     2881 cggaaaggtc tgatttatcc aacacggtgc taagtggcag cggacggaac acgaagcgaa
     2941 tctgattctc gtaattgcgc atgagctcgg caatggtctg tgccatggca aggcagccat
     3001 cagactgaaa atcgcaatat tctagaatag ttactggagc atcactggga ccaaaggaaa
     3061 aatctgctgc ggtgatggca ggcgcgagag actgctcgtt tggggagagc gtaggcatgg
     3121 cactgatcac cgtgcaggta tattgaggcg taggcgttgg cggaataaca ggatcgagca
     3181 cgatataagg tgtaggagta ttctctgtgc tacatgccgt gaaaaacaag gttatgccaa
     3241 agaatacaag cccgaagaga attcgtttca ttattatttg atagtttcct gtaattgttc
     3301 gaaagaccac atccaagcga tggtgttgat gaaatctttg agtgaggggc tttgttcgcg
     3361 caaggcacgc acggcgggac caaccgggtc ttcaccggcg gcgccaccgg gcacgtctgc
     3421 ataggcgccg tggaaggcga agtaggcttg gttgatctta cgaaggagat agccgttctg
     3481 taaaaagatt tggcggcgcg cttccatgta ggcttctgct tcgtcgattt tgccggctgc
     3541 tagtagggca tcggcagtga cgcgggtttc gtgcatttgc gcgcggaagt cgaagggcgg
     3601 gcgctctaag gtttcggggt cggggcggtc aaaggaaagc gagatcaagc tcaagttcgg
     3661 gaaagaggcg gcgggtttta attccgggta aaacttttcg atgactattg ctccaatttc
     3721 atcgccagca atcgatgc
//
LOCUS       DAOQFT010000215         3736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13825_length_3736_cov_5.741646, whole genome shotgun sequence.
ACCESSION   DAOQFT010000215 DAOQFT010000000
VERSION     DAOQFT010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13825_length_3736_cov_5.741646"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLR93_06575"
     CDS             <1..998
                     /locus_tag="PLR93_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH26667.1"
                     /translation="LLNRAAQGQLPLGSVFKIITFSAALESGLFLKDTTYDCQYDFTE
                     LQDQVRHDWTWQHCQDRLAAGLFCNTSDSTPSGLLTLQEGLMRSCNPYFWHIGNDLFT
                     NWDRGNDIANMARAFGLGSPTGIQQIPEEAGQIDDPATQVDAVNQAIGQGTVLVTPLQ
                     VVRFMAAIANGGTLYRPQIVEKIQPIDGDPIQSFKPEAQGTLPLRPENLDILREALLM
                     VVENPKGTARFNMRGLQFAVAGKTGTAESGSGKPHGWFAGYTMNEENTGLPDIAIVAV
                     GENIGEGSEYAVPFFRAMVETYYYGSPQRQYYDWGQIGYPPYTPTPPGGGVIPGG"
     gene            1039..1938
                     /gene="rsgA"
                     /locus_tag="PLR93_06580"
     CDS             1039..1938
                     /gene="rsgA"
                     /locus_tag="PLR93_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNH26668.1"
                     /translation="MANTKQGLIVKAQSGFFWVETGEGVIVCQLRGKLKQGRAKGDIA
                     ALGDQVDITVLDDGSGVVETVRERKQLIARLDPRPGGVYQQVLLANPDQAVFVFACAN
                     PNPKLRMLDRFLIIAEKQKLPVVIVANKIDLVENPKDNFGMYEAIGYHVVYTSTKNET
                     GLAELRNQLRNKLSAFAGPSGVGKSSLLNALQPGLGLAVNEISSVMNKGRHTTVVRQM
                     FAFEGGYVADTPGWKSLGLWDTEPEEIDAYFPELRGLVEQCQFSDCTHQHEPGCAVLT
                     ALKDGKIHAERYESFVRLRSGKE"
     gene            2017..3024
                     /gene="holB"
                     /locus_tag="PLR93_06585"
     CDS             2017..3024
                     /gene="holB"
                     /locus_tag="PLR93_06585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNH26669.1"
                     /translation="MTDNWNLLGHEWAVDMLRRHVARGEMRHAYLFAGAPGLGRRTLA
                     LRLAQALNCEKPVAPGEPCFVCRTCKQIDAMQHPDLAIIQAVDEDGVGKDGGTLRVDQ
                     IREVQRTLNLKPYQSPYRVAMFLRFQEANDNAANALLKTLEEAPAHAILLLTADNPEQ
                     LLPTINSRCEILRLRPLPLETIVADMLDHGVDEDRARLLAHISGGRPGFARKLLEDAT
                     VLEKREERLNDLQTILPASRVEKFSYAEKLAKDKDAMRQTILVWLSYWRDVLLRVAGA
                     ETPLINVDRNMEIEFLAGRLNLSTARKVVSDHESVLEKMERNVNSRLLAEVLLMDLPK
                     M"
     gene            3053..3490
                     /locus_tag="PLR93_06590"
     CDS             3053..3490
                     /locus_tag="PLR93_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26670.1"
                     /translation="MKKQNILFWASVAISALYGLIVTGFGIYLAGKGQDCLSYNDYAC
                     AFSGMFSFVIIPYGLAILLFVGIASLPFPVARLIGSILSALIGMGSVGLCCIVVLGAT
                     TTISTDGAILTGSEMAFFLFPFIMFLGGLALVGVGVFGYFRTK"
     gene            complement(3516..>3736)
                     /locus_tag="PLR93_06595"
     CDS             complement(3516..>3736)
                     /locus_tag="PLR93_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH26671.1"
                     /translation="SNIWQSQQKTVIMVTHSINESLFLADRVMVLTKRPGKIKMDVEV
                     DLPRPRTDDMRYTSHFGKLAKKLRSAIE"
BASE COUNT          925 a          845 c          947 g         1019 t
ORIGIN      
        1 cgcttctgaa ccgtgctgct cagggtcaac tcccattagg gtctgttttc aagattatta
       61 ccttctcggc agccctcgaa agcggactgt tcttaaaaga tacaacatac gattgtcagt
      121 atgattttac agaactacaa gaccaagtgc ggcatgattg gacgtggcaa cactgtcagg
      181 atcgtcttgc tgccgggtta ttctgtaata cgtctgatag tacaccttcg ggtttgctta
      241 ctttacaaga aggcttgatg cgttcttgta acccgtactt ctggcacatt ggcaacgatc
      301 tcttcacaaa ctgggatcgt ggtaacgaca tcgcgaacat ggcacgtgcg tttggtttgg
      361 gttctcccac cggcattcag cagattcctg aagaagcagg tcagatcgat gacccagcca
      421 ctcaggtaga tgcagtcaac caagcaattg gtcagggaac ggttttggtc acacctttgc
      481 aggtggttcg ctttatggca gcgattgcga atggcggtac cttataccgt ccacagattg
      541 ttgaaaagat tcagcccatc gatggcgatc ccattcaatc cttcaagcct gaagcgcagg
      601 gaacgctccc tttgcgccca gaaaacttag acattctgcg tgaagccttg ttgatggtgg
      661 tcgaaaaccc caaaggaacg gctcgcttca atatgcgtgg acttcaattt gcagtggctg
      721 gtaaaaccgg tactgccgag tctggtagcg gtaaaccaca tggttggttt gcaggctaca
      781 ccatgaacga agaaaataca ggcttgcctg atatcgccat tgtggcagta ggggagaaca
      841 tcggtgaagg ttctgaatat gccgtaccgt tcttccgcgc catggtggaa acctattact
      901 atggcagccc ccaacgccaa tactatgact ggggtcagat cggttacccg ccatatacgc
      961 ccactccgcc aggcggtggc gttattccgg gaggataaca tcaagaggca ggtatatacc
     1021 tgcctctaag gaattcactt ggcaaatacc aaacaaggac tcattgtaaa agcccaatcc
     1081 ggtttctttt gggtagaaac cggggagggc gttattgttt gtcaattacg aggcaaactc
     1141 aagcaaggca gagccaaagg cgatattgca gctttgggag atcaagtgga tatcacggtg
     1201 ttggatgacg gttccggcgt ggttgaaact gtccgtgagc gcaaacaact catcgcgcgg
     1261 ctcgatccgc gcccgggcgg ggtctatcaa caggtgcttt tagccaatcc cgatcaggct
     1321 gtcttcgtct tcgcatgcgc gaatccaaat cccaaattga gaatgctcga ccgttttctt
     1381 attattgcag aaaagcaaaa acttcctgtg gtaatcgtgg cgaataagat tgacttggtt
     1441 gagaatccaa aagataattt cggaatgtac gaagctattg gttatcatgt cgtttataca
     1501 tccaccaaaa atgagacagg tcttgcagag cttcgtaatc aattgcggaa taaactcagc
     1561 gcctttgccg gacccagcgg cgtgggcaag tcgagtttat tgaatgcgct acaacctggt
     1621 ttggggttgg ctgtgaacga gattagttct gtgatgaata agggacgtca caccacagtg
     1681 gtgcgccaaa tgtttgcctt tgaaggtggc tatgttgcag atacacccgg ttggaagtct
     1741 cttggcttgt gggataccga acccgaagag atcgatgctt acttccctga gttgcgcggg
     1801 ctggtagagc aatgtcaatt tagtgattgc acccatcaac atgagccggg ttgtgccgtg
     1861 ctcacagcat tgaaagatgg caagattcat gcagaacgat atgagtcttt tgtaaggctc
     1921 cgttctggca aagaataacg cgtagaaaat taccaaatac gtattgcatg ctttccacac
     1981 ttgacactta atctctgacc cctgatactt aaaccaatga ctgataactg gaatttactc
     2041 gggcacgaat gggcagtcga tatgcttcgc cgccatgtag cgcgtggaga gatgcgccat
     2101 gcctaccttt ttgcaggggc tccgggtttg ggtcgccgga cgttggcatt gcgcctagca
     2161 caagctttga actgtgagaa acctgttgca ccgggagagc cttgttttgt gtgccgcact
     2221 tgcaaacaga tcgacgccat gcaacaccca gatttagcga tcattcaagc cgtagacgaa
     2281 gacggagtgg ggaaggacgg tggcaccttg cgtgtggatc agatccgcga agtgcagcgg
     2341 acgttgaacc ttaagccgta tcaatctcca tatcgcgtgg ctatgttctt acggtttcaa
     2401 gaagcaaacg ataatgccgc caatgccttg cttaaaacct tggaggaagc cccggctcac
     2461 gctattttgc ttctaactgc cgataacccc gaacagcttt tacccaccat caactcgcgc
     2521 tgtgaaattt tacgtctgcg cccgttgccg ctcgaaacca tcgtcgcaga tatgctcgat
     2581 cacggcgtgg acgaagatcg tgctcgtttg ttggcgcaca tctcgggtgg tcgccctggg
     2641 tttgcgcgta aattgctgga agatgccact gtccttgaga agcgtgaaga acgtctcaat
     2701 gatttgcaaa ccattttgcc tgcatcacgc gttgaaaaat tttcttatgc cgagaagctt
     2761 gctaaagata aagatgccat gcgccagacc atccttgtat ggctttctta ttggcgagat
     2821 gttcttttgc gagtagcagg tgcagagact ccgcttatca atgtggaccg caatatggaa
     2881 attgaattcc tagcaggacg cttaaatctc tccaccgcgc gaaaagtagt cagcgatcat
     2941 gagtccgtct tagaaaagat ggagaggaat gtcaattcaa ggttgttggc agaagtattg
     3001 ttgatggatt tgccgaagat gtaagtttta gttttataaa aaataaagga aaatgaaaaa
     3061 acaaaacatt cttttttggg cgtcagtggc gatttctgct ctctatgggc tcattgtcac
     3121 cggatttgga atttatctgg caggcaaagg gcaagattgc ctgagctata acgattatgc
     3181 ctgtgccttc agtggaatgt tcagttttgt tattatcccc tatgggcttg ccattttatt
     3241 gttcgttgga attgcaagct tgccttttcc cgttgcccgt ctgattggat ccattctgtc
     3301 tgcattgatt ggcatgggaa gcgttggctt gtgctgtatt gttgtccttg gggcaacgac
     3361 cacaataagc acagacggcg ctattttaac aggcagtgaa atggctttct ttctattccc
     3421 atttattatg tttctcggcg gtcttgcatt ggttggcgtt ggtgtatttg gttattttag
     3481 aacaaagtaa ccctgtaaag ttgtggaaat ttgatttatt caatggctga tcttaatttc
     3541 tttgccaatt tcccgaagtg acttgtatat ctcatatcgt ctgtgcgtgg acgtggaagg
     3601 tcgacttcca cgtccatttt tattttaccg gggcgtttcg ttaacaccat cacacggtct
     3661 gcaagaaata aagactcatt aatggaatga gtcaccatga tgaccgtctt ctgttgagat
     3721 tgccaaatgt tggaga
//
LOCUS       DAOQFT010000216         3709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_13992_length_3709_cov_7.199781, whole genome shotgun sequence.
ACCESSION   DAOQFT010000216 DAOQFT010000000
VERSION     DAOQFT010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13992_length_3709_cov_7.199781"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..645
                     /locus_tag="PLR93_06600"
     CDS             37..645
                     /locus_tag="PLR93_06600"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH26672.1"
                     /translation="MNEEQTWVLQAQQGNDEAFTKLVETYQTPVFNLCYRMLGEPELA
                     EDAAQETFLRAYQHLHRYDQKRPFPTWLLSIAAHYCIDRLRRRKFSMFSMDTEDEEGN
                     SFEIPDVDAPNPEGEAITGQTNERVHAMLKDLDATDRAAIIMRYWYDYSEKEIAESLN
                     LTVSAVKSRLHRARKELAGLWAEQEDDLLAEMERRHHESPAF"
     gene            626..1156
                     /locus_tag="PLR93_06605"
     CDS             626..1156
                     /locus_tag="PLR93_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNH26673.1"
                     /translation="MNHQPFETWLLDDKVLTPTEKRELDSHLRTCKNCTALAETGLAL
                     RSSRVATPAPGFAMRFQQKLAAQKLAEQRRRLWGMIVLIVSGVGLLGWFLAPVLMSIT
                     TSPVGFLLSAAGLFAFLFTSIQAFGEVLTVMLRMLPAFLPPYMWMVLFSGAAGIGLLW
                     AVSIWRLVRRPQGVFA"
     gene            1153..>1490
                     /locus_tag="PLR93_06610"
     CDS             1153..>1490
                     /locus_tag="PLR93_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26674.1"
                     /translation="MKKSTLTRILLLLALLIVPTASIHAQTPNNDDVVLFGQNYTLEE
                     GETLNGSLAVFGGNIDIKEGAEVNGSIAIFGGNITFAKDTTINGDVAVFGGNVSVEGE
                     IAGDMVLFGG"
     assembly_gap    1491..1590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1591..1994
                     /locus_tag="PLR93_06615"
     CDS             <1591..1994
                     /locus_tag="PLR93_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26675.1"
                     /translation="AGGYGLIVFFALPIALVIMVITLILIPVAAIVVLLIPLAWLFGI
                     VALGQEVGERFSKAINQVWAPVLSTGLGTFLLMLVIGFLELIPCFGWIPSAAVTLISV
                     GAAVMTWFGTRNPPGYTPAAAAVVEEVPPAS"
     gene            complement(2051..3184)
                     /locus_tag="PLR93_06620"
     CDS             complement(2051..3184)
                     /locus_tag="PLR93_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26676.1"
                     /translation="MPIFYDPIFSFISLGHFMVDTFNASRPVLLTYFGLSESQIAFFS
                     TIYIWASALTQPVFGWISDRTGPRWLAAGGVLWMTVFYSLALTLPGNLGLGCLILAAL
                     GSSAFHPVAAVQATLRGREFLKGRETTATSLFFTAGQMGHFVGPIITGLILAYLKIPG
                     MYILPVVSIPIGFALIYQLKDNHPHPQPVKTVISRRNRAGITFILVLMAVATMQSWAQ
                     ANMVNLIPKYIKDLGQGATVYGSMAGLFMGGSALGNMIGGHFADRYPKRFVASLALLF
                     SAIPIFVISRIGWSGWLYALVPLAGAGTGAVHSIMVVLAQRIIPGGMALASGLILGFI
                     FSSGALGLLYTGHLAEVYGFPYVLVLTTGMVLVAAPLALLLKE"
     gene            complement(3208..>3709)
                     /gene="nadD"
                     /locus_tag="PLR93_06625"
     CDS             complement(3208..>3709)
                     /gene="nadD"
                     /locus_tag="PLR93_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNH26677.1"
                     /translation="FLWMLTPDPPHKLNTFITPLSHRLPMLQAMIAHDPVFELSRVEI
                     DRPGPHYTIDTVRFLTQQYGDAEIYLLIGGDSLRDLPSWRLSADLVAEVSKIGVMRRP
                     GDFTDLTPIEDKLPGVTSKIHFIEALLQPISSREIRRRVASGEMYRYYLAPEVYEYIE
                     THHLYR"
BASE COUNT          943 a          933 c          915 g          818 t
ORIGIN      
        1 cgtaagagaa ggtagaaagc cttcccgagg agaaacgtga acgaagaaca gacctgggtt
       61 ctccaggcgc aacaaggtaa tgacgaggcg tttaccaaac tcgttgaaac ctaccaaacc
      121 cctgttttca acctgtgtta ccgcatgttg ggtgaaccag agctcgcaga agatgctgct
      181 caggaaacct tcctgcgcgc ctatcaacat ttacacaggt atgaccagaa acgccccttc
      241 ccgacttggt tgctctcgat cgcggcgcat tactgcattg accgtcttcg ccgccgcaag
      301 ttctcaatgt tctcgatgga taccgaagac gaagaaggta actcgttcga aattcccgat
      361 gtggatgcgc ccaaccctga aggggaagcc atcaccgggc agaccaatga acgagtccat
      421 gccatgctca aagacttgga tgccaccgac cgtgcggcaa tcattatgag atattggtat
      481 gactattcag aaaaggaaat tgccgagtcc ttgaatttga cggtgagtgc ggtgaaaagc
      541 cgcctgcacc gagcacgaaa ggaactggct ggcttgtggg ctgaacaaga agatgacctg
      601 ctcgccgaaa tggaaaggag acaccatgaa tcaccagcct tttgaaacct ggctgttgga
      661 cgataaagtc ctaaccccta cagagaaacg tgaactcgac tctcatctgc gcacttgcaa
      721 gaattgcacc gcactggcag agaccgggct tgccttacgc tcttctcggg tagcaacccc
      781 tgcacctggg tttgccatgc gcttccagca aaaactggcg gcgcaaaagc tggcagaaca
      841 acgccgccgt ctatggggca tgatcgtgct catcgtcagc ggagttgggt tactcggatg
      901 gtttcttgct cccgttttga tgtcgatcac aacctcgccg gtgggctttt tgctctctgc
      961 tgccgggtta tttgcatttc tattcacatc catacaagca ttcggtgaag tgctcactgt
     1021 aatgctgcgc atgcttcctg cttttctccc tccctatatg tggatggttc tcttctctgg
     1081 agcagcgggt attgggttgt tatgggcagt atccatctgg cgcctcgttc gccgtcctca
     1141 aggagttttt gcatgaaaaa atcaacgcta acccgaatcc tgcttctgct ggcacttttg
     1201 atcgtgccta cagcaagcat ccacgcgcag accccgaata atgatgatgt cgttctattc
     1261 ggtcaaaact acacgctgga agagggtgaa acgctgaatg gcagccttgc tgtttttggc
     1321 ggcaatattg atattaaaga aggggcagag gtcaacgggt cgatcgccat ctttggcggc
     1381 aatatcacct tcgcaaaaga caccaccatc aatggtgatg tagccgtctt tggtggaaat
     1441 gtcagcgttg aaggtgagat cgcaggcgat atggtgctct ttggcgggca nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggctggcgg ctacggcttg attgtattct
     1621 ttgccctgcc gattgcattg gtcattatgg tcattacgtt aatcttgatc cccgttgcgg
     1681 caattgtcgt cttgcttatc cccttggcat ggctattcgg catcgtggcg ttgggtcagg
     1741 aagttggtga acgctttagc aaagccatca accaagtttg ggcaccggtt ctttccaccg
     1801 gtctcggaac cttcttgctg atgctggtga tcggtttcct tgaactcatt ccatgcttcg
     1861 gttggatccc ctccgcagct gtcaccctaa tcagtgtggg tgccgccgtg atgacgtggt
     1921 tcggaacgcg taacccaccg ggatacaccc ccgccgcggc ggcggttgtg gaagaagttc
     1981 cccccgcctc ttaattggac tgtagcctgc tacaaaccat ccgccttggt caacacccaa
     2041 ggcggatttt ttattccttc aataataacg ccaacggtgc cgccacaagg accatacctg
     2101 tcgtcaacac cagtacatac gggaagccat aaacctctgc gagatgaccg gtatacagca
     2161 accctaacgc ccccgaagaa aagataaagc cgagaatcaa ccctgaagcc agagccatgc
     2221 cgccaggaat gatgcgttgt gccaagacca ccatgatgct atgcacggcg cccgtccctg
     2281 cccctgcgag tggaaccaaa gcatataacc acccagacca accgattcgg ctaatcacaa
     2341 agatcggaat ggcagagaaa agtaaagcca atgacgccac aaaccgcttg ggatatcggt
     2401 ctgcaaagtg cccgccgatc atatttccta aagccgagcc acccatgaag agtcctgcca
     2461 tgctgccata gaccgtggcg ccctgcccca aatctttgat gtattttggg atgaggttca
     2521 ccatattcgc ctgtgcccaa gattgcatgg tggcaactgc cattaagacc agaataaatg
     2581 tgatgcctgc acgattgcgt ctagaaatga ccgtcttgac tggttgcggg tgtggatgat
     2641 tatctttcaa ctggtatatc aacgcaaagc caatgggaat ggataccaca ggcaagatat
     2701 acatgccagg aattttcaaa tatgccaaga tcaatccagt aataatagga cctacaaaat
     2761 gtcccatttg cccagcggtg aaaaataatg aagttgcggt tgtctctcgc cctttcaaga
     2821 attcacgtcc gcgcaaagtg gcttggaccg cagccacagg gtgaaacgca gaagacccca
     2881 acgccgcaag gataagacaa cccaagccaa gatttcccgg taacgttagc gcgagtgaat
     2941 agaaaacggt catccacaaa acgccaccgg ctgccaacca acgcggaccc gtccgatctg
     3001 agatccagcc gaagactggt tgtgtcaatg cagatgccca aatatagatc gtagaaaaaa
     3061 aagcaatttg tgattcgctc aaaccgaaat aggtcaacaa caccggtcgg ctggcattga
     3121 aggtatccac cataaagtga ccgagagaga taaaagaaaa tatagggtcg taaaaaatag
     3181 gcataggaaa attataaata gtgaatacta acgatagaga tgatgggtct caatatactc
     3241 gtacacttca ggcgcaagat aataacgata catttcccca gaagccacac gcctgcgaat
     3301 ttcacgggaa gagatgggct gcaacaaggc ttcgataaaa tggatcttgg atgtgacccc
     3361 gggaagttta tcttctatcg gggtgaggtc ggtgaagtcg cctgggcggc gcatcacgcc
     3421 aattttggaa acttcagcca cgaggtccgc gctgaggcgc caacttggca ggtctcgcag
     3481 agaatcgccg ccgatcagca agtagatttc agcatcccca tattgttgtg tcagaaaccg
     3541 gacggtgtca attgtgtaat gtggacccgg tctatcgatc tcgacacgcg acaactcaaa
     3601 cacagggtcg tgagcgatca tcgcctgcaa catcggaaga cgatgagaga gaggcgtgat
     3661 gaaggtattc aatttatgcg gtgggtctgg ggtaagcatc cacagaaat
//
LOCUS       DAOQFT010000217         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14053_length_3700_cov_6.906996, whole genome shotgun sequence.
ACCESSION   DAOQFT010000217 DAOQFT010000000
VERSION     DAOQFT010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14053_length_3700_cov_6.906996"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1771
                     /locus_tag="PLR93_06630"
     CDS             221..1771
                     /locus_tag="PLR93_06630"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNH26678.1"
                     /translation="MQTTKRPFALALFASLISTLLFSCSEQENTATLSVEITQTAEPT
                     VLPSPTSTPLVAANENPLTGLPVKDASLLDLPALLVSISHFPVEARPQAGLSFAPWVF
                     EVYITEGATRFLTIFHGLFPEPEIPVTGDCAIRNEPFTQTSTIIGNRVWLDENANNIQ
                     EAWEKGIGGICIYLYREDGTLLYQTSTDSNGYYAFNVEPGRYVLDFQAPPWMQFVQKN
                     SGDEETDSNVDPATGQTEALDVTSSLHHIDAGLVLTSQPSTTSTLPAAKVGPVRSGRL
                     IYADIAAFFPDSCLIYAFASPEVLELLPQCYFVQHDLEGGGYMLDIDELKRLAQESKQ
                     YDVDYASNAFDENIPEGGEPASRLDVYFAYLNQSAWVYDAAAESYWRYVDNADYDNPG
                     VLHPEIDRLTGRQLQFENVVVLFTEHDVISPTNLDIHLEEDKEGYALLFRDGRMYDMF
                     WSTDLSDAERESGRHKPIHFINPDTKQLMPLKPGRTWIIVVTPETTVSPQADGKWYLE
                     FYQPAGAQ"
     gene            1789..3393
                     /locus_tag="PLR93_06635"
     CDS             1789..3393
                     /locus_tag="PLR93_06635"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNH26679.1"
                     /translation="MKRITSLIWIACLLCACAGSATVETTIENTATVLPSATPTPIFT
                     PTPQPHSQPISYGPDRENFPSNVNPLSGLQVEDPSLLKTPAIALSISNFPVAARPQAG
                     LSFAPWVFEFYITEGATRFLSIFHGAFPEPEAPIVGDCEVRREPIQGNVIEIGNRIWL
                     DENQNGIQEDYEKGVGGVCINLLDATGKSVQKTTSDSNGFYGFVTEPGNYIVEVTIPE
                     WLKFTKPNVGDEEHDSDADPVSGKIEADLISTPLDLDVGLVPSEKANPPVKQSAQLPP
                     AEVGPIRSGRLLYVDVHSMFLNSCFVFASASEEILPLLPQCYLVPHDEASGGAMLSIE
                     KMKSLAEENKGTRPVDFNYASNVFAETPPEGGETALRLNSYVALLNQSAWVYDPASSS
                     YWRYVDDTTQANAGVLHPEIDRLTGRQLQFNNIIVLEVDQEVYEYQDRPIRTLLDYHL
                     ELGQGGYAYLFRDGRKYDIRWTTKARAYEKQTGQARPLYFVDANGDPVSLKPGNTWFI
                     VATPYSYFTPEDGVWNFRFVPPEGVK"
     gene            3405..>3700
                     /locus_tag="PLR93_06640"
     CDS             3405..>3700
                     /locus_tag="PLR93_06640"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNH26680.1"
                     /translation="MHPYVRSLKILFEANANPVNAAPMKKYMRDQFEYLGIKSPQVKS
                     LFSGYIKQNSLPPVEDLSTIVRELWNLPQREFQYLAINLLDKMEKKLPEDFIVTL"
BASE COUNT          988 a          926 c          845 g          941 t
ORIGIN      
        1 gaagccgccg gtgccggcgt tttggggcac gtccacattc acattgtgcc acgctgggca
       61 ggcgatacaa acttcatgtc ctccgtcggt gagacgcgcg tcttacccga atctctagag
      121 caaacctatc aacgagtcca tagcgcgttc aattcatcat aaccaaaacg tcgtttagct
      181 ggaattaatc atcatggggg aatattggtt tggagatttt atgcaaacta ccaaacgacc
      241 tttcgcgctt gctctttttg cgtctcttat ttcaacgctg ttgttttctt gttcagaaca
      301 agaaaacaca gcaaccttgt ctgtagaaat aacccaaact gcagaaccta ctgttttgcc
      361 cagcccaaca tccacgccgc ttgtggctgc aaatgaaaac ccattgaccg gcttgcctgt
      421 gaaagatgcc agcttgcttg accttccagc cttgcttgtg tccatttcac attttccagt
      481 agaggcacgt ccgcaagcgg ggctatcgtt cgcgccatgg gtttttgagg tttacattac
      541 cgaaggggct acacgttttc tcactatatt tcatgggctc ttccccgagc cagaaattcc
      601 agtcactgga gattgcgcaa ttcgcaatga gccatttacg caaacctcta cgatcatcgg
      661 aaatcgcgtc tggttggatg aaaatgccaa caacatacaa gaagcgtggg aaaaaggcat
      721 cggcggaatt tgtatttacc tgtatcgcga agacggcact ttactgtatc aaacctccac
      781 agatagcaac ggctattatg cgtttaacgt cgagccgggc agatatgtgc ttgattttca
      841 agccccacca tggatgcagt tcgttcagaa aaattctggg gatgaagaaa ctgatagcaa
      901 cgtggaccca gccaccggtc agaccgaggc gttggatgta acatcctcgc ttcatcacat
      961 agacgcaggc ctggtcctca cctctcagcc ttctactaca tccacacttc ctgctgccaa
     1021 agtaggaccc gtgcgttcgg ggcgtttgat ctacgcagac atcgccgcat tcttcccaga
     1081 ttcttgtctc atctatgcct tcgcctcacc cgaagtattg gaacttttgc cgcaatgtta
     1141 ttttgttcaa catgatttgg aaggcggcgg ctacatgttg gatattgacg aactcaaacg
     1201 cttggcacaa gaaagtaaac aatatgacgt ggattacgcc agcaatgcct tcgatgaaaa
     1261 tattcccgaa ggtggtgagc ctgcatcacg cttggatgtg tattttgcct atctcaatca
     1321 atctgcatgg gtgtatgatg cggcggctga aagttattgg cgctatgtgg acaatgccga
     1381 ttatgacaac cccggtgttc tccaccccga aattgaccga ttgacaggac gccaacttca
     1441 atttgaaaat gttgtggtgt tattcacaga acatgatgtt atctctccca ccaacctcga
     1501 cattcacctc gaagaagata aagaaggcta tgccttatta ttccgtgatg gacgcatgta
     1561 tgatatgttt tggagcacag acctgagcga cgcagaacgt gaaagcggaa gacataaacc
     1621 tattcacttc atcaacccag ataccaaaca actcatgccg ctcaaacccg gtcgcacatg
     1681 gatcatcgtc gtcacccctg aaacgacagt atcaccgcaa gccgatggca agtggtattt
     1741 ggaattttat caaccggctg gggcacaata agttttggag ttttcatctt gaaaagaatt
     1801 acatcgttga tctggatagc atgtttactc tgcgcgtgtg ctggcagcgc aaccgttgaa
     1861 acaactatcg aaaataccgc tacagtctta ccgtctgcaa cgcccacgcc tatcttcaca
     1921 cctacacctc aacctcacag ccagcctatc tcatatggtc ccgaccgcga aaattttccg
     1981 tcgaatgtaa atccgctttc ggggttgcaa gtggaagatc caagcttatt aaaaactcca
     2041 gccatagcac tctccatttc aaattttccc gtagcggctc gcccacaggc agggttatcg
     2101 tttgcgccat gggtctttga gttttacatc accgaaggcg caacccgctt cctctccatt
     2161 tttcacggag catttcccga gccagaagct cccatcgttg gcgattgcga agtgaggcgt
     2221 gagcccattc aaggcaatgt tattgagatc ggaaatcgta tctggttgga tgaaaaccaa
     2281 aacggcattc aagaagatta cgaaaaaggt gtggggggag tctgtatcaa tttattggat
     2341 gcgaccggga aatcagttca gaaaacaacc agcgactcaa acggttttta cggcttcgtc
     2401 acagagccgg gaaactatat cgttgaagtg accattccag agtggttgaa atttacgaag
     2461 ccaaatgttg gagatgagga acatgacagt gacgcggacc ctgtttcggg gaagatcgaa
     2521 gcggatctta tctccacgcc tcttgactta gacgtgggct tggttccgtc cgaaaaagcg
     2581 aatccacccg tgaagcaatc agcccaactc ccccccgctg aggtgggacc catccgctcg
     2641 ggacgcttgt tatacgtgga tgttcacagc atgttcttaa attcatgttt tgtctttgca
     2701 tcggcatcgg aagaaattct gccattgttg ccacaatgct atttggtgcc acatgacgaa
     2761 gccagtggcg gtgcaatgct aagcattgaa aagatgaaat cgctggcaga agagaataaa
     2821 ggcacacgac cggttgattt caattacgcc agcaatgttt tcgcagagac gccacccgaa
     2881 ggtggtgaga cagcgctgcg tttaaattct tatgtagcct tactcaatca atctgcatgg
     2941 gtgtatgacc ccgcctccag ttcgtattgg cgttatgtag atgacacgac acaagcaaat
     3001 gccggagtct tacatccaga aatagaccgc ctgacaggca ggcagctaca attcaataac
     3061 atcatcgtat tggaagttga ccaggaagtc tatgagtatc aggatagacc catccgcacc
     3121 ttgctagact atcacctcga acttgggcaa ggtgggtatg cttatctctt tcgagatggc
     3181 agaaaatatg atattcgctg gacaaccaaa gcccgtgctt atgaaaaaca aaccgggcaa
     3241 gcccgcccgt tatattttgt agatgcgaat ggagacccag tctccttaaa gccaggcaat
     3301 acctggttca tcgttgccac gccctattcg tactttacgc ccgaagatgg cgtttggaat
     3361 ttccgttttg tgccgcctga aggtgtgaaa taaaagagga aattatgcac ccttatgtcc
     3421 gttctttgaa aatacttttt gaagccaatg ccaaccccgt caacgccgcg ccgatgaaga
     3481 aatatatgcg cgaccaattt gagtatctgg gaataaaaag cccgcaagtg aagagtctgt
     3541 tttctggata catcaagcaa aacagtttgc cgcctgtaga agacttaagc accatcgtcc
     3601 gcgaattatg gaatctccct caacgcgagt ttcagtacct tgccattaac ttgctcgata
     3661 aaatggaaaa gaaactacct gaagatttca ttgttacgct
//
LOCUS       DAOQFT010000218         3699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14063_length_3699_cov_6.148738, whole genome shotgun sequence.
ACCESSION   DAOQFT010000218 DAOQFT010000000
VERSION     DAOQFT010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14063_length_3699_cov_6.148738"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2150
                     /locus_tag="PLR93_06645"
     CDS             <1..2150
                     /locus_tag="PLR93_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH26681.1"
                     /translation="QKTPASSHIAGNLPRPLTSFIGREEELNEVETLLAETRFLTLLG
                     AGGSGKTRLAIQAADESAHNYPDGVWWVDLAPLLKGDQLPQAIAKSLGVKEKLGADML
                     DHIASFIGERKMLIILDNCEHLVSATAHSIEAVSAKCQAVTFLATSREPLGVASEIGW
                     QTPAFPLPEPGASHLKTLAKNESVRLFVERGRAVNGSFQLNGQNVMRVAEICRRLEGM
                     PLAIELAAAQVNNLTLDGILSRLEKSLNLTSTDAAHARHAALHSAIEWSYNLLTPAEQ
                     MLFRRLAIFSGGWNLASAAIVAAGYSDPEAPLSNEASEGTIAPLPINGETVTRQILDA
                     LVKKALAQFRRAEGSLRYDTLDTLREFAREKMNEVGETQIMQERHALTFFNFLKKAWD
                     DVYSERESSIFNQIDLEYANLRTAWAWQLQSPKWVVDAKAINSQNRFWYVRGHYAEGR
                     ETLEALLKHPDVHLNTLIHATLLNNLGLIEWKSGNRIAAKRIFEEALVEFRTLQLDFA
                     AAMIIANLAGLYIDDDDMEKGLPLAHEAVVVARETGNKSALNISLNNLAIALTITRDF
                     KGAREAFYEGLALSKEQGDIRGEGIIYNGLADIEAEEGNFAEARRLFTQSFMTRSSLG
                     DRRGSIIPALNLIRLMCMDEDWENAACLLGFVEETLQQLGIQLASDARAGREEANRSV
                     RLALDEETVTRALQFGKTLTLDSANRWLAEKSL"
     gene            2208..2849
                     /locus_tag="PLR93_06650"
     CDS             2208..2849
                     /locus_tag="PLR93_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26682.1"
                     /translation="MKQYRMLFAFAALMLVVSLACGGSSAPATENPTLPPVPTNPPAQ
                     QEQPQGQDQSQGQTTTDSGLVTFVDENGLAAFDLPGDWVYSHSTGDNYYVDRFTTPDG
                     FSGLVESLVYNDGTSFVKSQKGQFALDLINTFYSATGEVGDIKVTSDQIMPDGSERLE
                     WTSKSGGYSGMTFFETRGDDNSTFMMLSAWWDNNADQATLDIINNAIASYHIP"
     gene            2907..3128
                     /locus_tag="PLR93_06655"
     CDS             2907..3128
                     /locus_tag="PLR93_06655"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HNH26683.1"
                     /translation="MSAQAIESILTRAMNDVTFADMFFANPDLTLAGFDLTAEEIVRL
                     KSMSRSELENATPETRLDIIKKLQSQDSS"
     gene            3180..>3699
                     /locus_tag="PLR93_06660"
     CDS             3180..>3699
                     /locus_tag="PLR93_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNH26684.1"
                     /translation="MATTIFSPYRICPIGAHIDHQGGAVLGRTIQLGTTLTYEPNTFK
                     EIHLISARYGETAFTIGDLDLSHWARYAQAAARVLKPTVGMHAQIHGSLIGAGLSSSA
                     SVGLAYLKALADVNHIGLTSEQLIQLDFELEHDQLGLQNGLLDPMSIVHGKKNALLFM
                     DVVTGSVSPVFDS"
BASE COUNT          964 a          977 c          869 g          889 t
ORIGIN      
        1 tgcaaaagac acctgcatct tcccatatag caggcaacct cccccgccca ttgaccagct
       61 ttattgggcg cgaagaagaa ctgaatgaag tagaaacctt attagcagaa actcgcttcc
      121 ttaccttatt gggtgcaggt ggaagtggca agacccggct tgccattcaa gcagctgatg
      181 agtctgcaca taactatcct gacggggtct ggtgggttga ccttgctccc ctgctcaaag
      241 gagaccaatt gccgcaagcc attgcgaaat cgcttggggt aaaagaaaag ctcggcgccg
      301 atatgcttga ccacattgca agttttatcg gcgaacgcaa gatgctaatc atcttggata
      361 actgtgaaca tcttgtctcc gctactgctc acagtatcga agcagtatcc gccaaatgcc
      421 aggcagtgac ctttcttgct accagccgtg agccgcttgg tgttgcaagt gaaattggct
      481 ggcaaacacc agcattccct ctacctgaac ctggcgcgag tcatctcaag actcttgcaa
      541 agaacgaatc ggtgcggctc ttcgtcgagc gtggacgggc cgtcaatggc agctttcagc
      601 tcaacggtca gaatgtgatg cgtgtggcag agatttgtcg ccgcctcgaa gggatgccat
      661 tagcgatcga gcttgccgct gcacaagtca acaatctcac gttagatggc atcttatcgc
      721 gcttggaaaa gtcgttgaac ttaacctcaa ccgatgccgc tcatgctcgt cacgctgcat
      781 tgcattctgc cattgaatgg agttacaacc tgcttacccc agcagaacaa atgctttttc
      841 gtcggctggc aattttttct ggcggatgga atctcgcctc agccgccatt gtagcggcag
      901 gctattctga ccccgaagcg cctctttcga atgaagcttc agaaggaaca atcgcaccat
      961 tgcccatcaa tggagaaacg gttacacgac agattctgga tgcacttgta aaaaaagcat
     1021 tagcacaatt ccgtcgagca gaaggcagct tgcgatacga cacgttagac accttacgag
     1081 aatttgcacg cgaaaaaatg aatgaagttg gtgaaaccca gatcatgcaa gaacggcatg
     1141 ctctcacatt ttttaatttt ctaaaaaaag cttgggatga tgtttattca gaacgcgaat
     1201 caagcatatt caatcaaata gatttggaat atgccaatct gcgcaccgca tgggcatggc
     1261 aactgcaatc gccaaaatgg gtggtagatg ccaaggcaat taatagccag aaccgttttt
     1321 ggtatgtacg cggacattac gctgaaggac gtgaaactct cgaagccttg ctcaaacatc
     1381 ctgatgtaca tttaaataca ctcatccatg ccactttgct taacaacctt ggtttaatcg
     1441 aatggaaaag cggaaaccgt attgcagcaa aaaggatttt cgaagaagca ttagtagaat
     1501 ttcgaaccct acaattggat tttgccgccg caatgatcat tgcgaacctg gctgggcttt
     1561 atatcgatga cgatgacatg gaaaaggggc ttcccctggc gcacgaagcc gtggtcgtgg
     1621 cacgtgaaac cggaaataaa agtgccctca acattagttt gaacaatctt gccattgcgc
     1681 tcaccatcac tcgtgatttc aaaggcgctc gcgaagcttt ctatgaaggt cttgctttga
     1741 gcaaagaaca aggcgatatc cgtggcgaag gtattatcta caacggcttg gcagatatcg
     1801 aggcggaaga aggcaatttt gcagaagccc gccgcctgtt cacccaaagc tttatgacgc
     1861 gctccagcct cggcgaccgc cgtggttcca tcatccctgc actgaacttg atccgcttaa
     1921 tgtgcatgga tgaagattgg gaaaatgccg cctgtttgct agggtttgtg gaagaaaccc
     1981 tacaacaact cgggattcaa cttgcatctg atgcacgtgc tgggcgtgaa gaagcaaatc
     2041 gttccgtccg cctcgcgctg gatgaggaaa ctgttactcg tgcccttcaa tttggcaaaa
     2101 cactgacact tgattcggca aaccgctggc tggcagaaaa atccctgtaa cgcttatgta
     2161 acggtacgtt tctatcatag aggaaattca acaaaggaga atttctcatg aaacaatacc
     2221 gtatgttatt tgcttttgct gcgctgatgc ttgtcgtcag cctcgcctgt ggtggctcgt
     2281 cggcacctgc cactgaaaac cccacactgc cccccgtccc taccaacccg cctgctcagc
     2341 aggaacagcc ccaaggacag gatcaatcac aaggtcaaac caccacagac agtggcttag
     2401 tcaccttcgt ggacgagaat ggtcttgccg ccttcgacct tcccggcgat tgggtctaca
     2461 gtcactccac cggtgacaat tactatgtcg accgcttcac cacccccgac ggcttcagcg
     2521 gcttagtaga gagccttgtc tacaacgacg gaacctcttt cgtcaaaagc caaaagggac
     2581 aattcgccct cgatctcatt aacacttttt atagcgccac cggagaagtc ggtgacatca
     2641 aagtcaccag cgatcagatc atgcccgatg ggtccgaacg tctcgaatgg acttccaaaa
     2701 gcggcggcta ctctggtatg accttcttcg aaacccgtgg cgacgataac agcaccttca
     2761 tgatgctctc tgcttggtgg gataacaacg ctgaccaagc cactttagat atcatcaata
     2821 acgccattgc ctcttatcat attccgtaaa taaacccaca aaacgagcag gcaggtcaac
     2881 ctgcctgctc gtttcaagga cattccatgt ctgctcaagc catcgaaagc attttgactc
     2941 gtgccatgaa cgacgtgacc ttcgcagata tgttctttgc caacccagac ttaaccttag
     3001 caggcttcga cctaaccgct gaagagatcg taagactcaa gagcatgtct cgttcagaac
     3061 ttgagaatgc caccccagaa acacggctgg acattattaa gaaactccag tcacaagatt
     3121 cgagctaaaa gagcatctcg caaagatgct cttttttttc atgttagact cgccgcgtta
     3181 tggcaacaac catcttttct ccgtatcgca tttgtcccat cggcgcgcac atcgaccatc
     3241 agggcggcgc agtgttggga cgcacgatcc agcttggcac cacacttacc tatgaaccga
     3301 acactttcaa agagattcat cttatcagcg cgcgctacgg cgaaacagct tttaccatcg
     3361 gcgatcttga tctttcgcat tgggcacgct atgcccaggc ggcggcgcgg gttctgaagc
     3421 caacggttgg catgcatgcc caaatccatg gttcgttgat cggcgcaggc ttgagttcat
     3481 ctgcttcggt agggttggcg tatctcaaag cgcttgcgga tgtaaatcat attggactca
     3541 ctagcgagca gcttatccaa ctcgattttg aattggagca tgaccaactc gggctgcaaa
     3601 acgggctcct cgacccgatg agcattgtgc atggaaaaaa gaacgcgctt cttttcatgg
     3661 acgtggtgac tggttctgtt tccccggtct ttgactccg
//
LOCUS       DAOQFT010000219         3690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14107_length_3690_cov_4.944154, whole genome shotgun sequence.
ACCESSION   DAOQFT010000219 DAOQFT010000000
VERSION     DAOQFT010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14107_length_3690_cov_4.944154"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLR93_06665"
     CDS             complement(<1..858)
                     /locus_tag="PLR93_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26685.1"
                     /translation="MKAFGRFFAQLAFYTISIGLLVYAASRSLHFIQASLDEDNQILG
                     MFALLATSGGMIGWLTIFLFKADGIVQKGIALLMVLVDFLGEAALFTMDTLQQAGANG
                     VVKELTQSEARTVLIGMSILIALNILAGIAYHLGNMEIIRQMAEGAQKDILFFKSLAE
                     IEKNTDDLARTMAPSIASEWKTEFTKNYGTASKLGLTGGKNESASSPAAPKSSSPTKL
                     RLPRFNILPWKKTEKPATNNSQPAFFPFTAKLQTNLNNHVVKPGEQLEAGIAANGNYI
                     IRVPGTAQPI"
     gene            complement(861..1259)
                     /locus_tag="PLR93_06670"
     CDS             complement(861..1259)
                     /locus_tag="PLR93_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH26686.1"
                     /translation="MKIKPSKYPSIKYRSLALWAISLCAISLACSLTDSSSRPSVTPT
                     SSPIARDYANPIEAKQTLDLSAISSQTSCTVSATVLHLRSCAGTNCPVDAWLLQGESL
                     TILQTGTTWINVQTSNGKTGWVNATYCGGK"
     gene            complement(1492..1734)
                     /locus_tag="PLR93_06675"
     CDS             complement(1492..1734)
                     /locus_tag="PLR93_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH26687.1"
                     /translation="MTAGKIPSDEEAILSVEFLTVAQVARWARVSTKTVYRWIEAKKL
                     PAIKFGKRTYRIPTRAVIEYLKSAGYADLIAQPKPK"
     gene            complement(1836..1909)
                     /locus_tag="PLR93_06680"
     tRNA            complement(1836..1909)
                     /locus_tag="PLR93_06680"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1873..1875),aa:Asp,seq:gtc)
     gene            2168..2539
                     /locus_tag="PLR93_06685"
     CDS             2168..2539
                     /locus_tag="PLR93_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNH26688.1"
                     /translation="MSKNRTQQMVDRLRELQASSPDIEASAIVSVDGLSIASALPQGV
                     EEDRVSAMSAAMLSLGERIAGELGRGSLEQVYIKGLKGYVVLISVGEEAVLTALVREQ
                     ARLGLIFLDMRRAAEDLAKLI"
     gene            2550..3185
                     /locus_tag="PLR93_06690"
     CDS             2550..3185
                     /locus_tag="PLR93_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNH26689.1"
                     /translation="MSSVQKSGLYYPNKFGLITIKSLEEVMGKNGLNAILNLGGLSHY
                     IENYPPDNLDKGFDFTELSAIGSSLEEMYGPRGGRGLALRAGRATFADALRNFGALAG
                     AADLAFVVLPLQSKLRIGLPAFAKIFTQLSDQQTTVEEKDTEWVWTIHKCPCCWGRTG
                     VDKPVCFIATGLLQESLKWVSGGNEFRVNESKCIAMGDPVCEFIVQKEPIS"
     gene            3202..>3690
                     /locus_tag="PLR93_06695"
     CDS             3202..>3690
                     /locus_tag="PLR93_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26690.1"
                     /translation="MADAKSKILIVEDDPDVAEMLTAYFRTQDYEVFTVNWGEDGVRS
                     ALQVNPDLAILDIRLPDIDGYEVARRLRTDRRTAQIPIIFLTEKRDRADRLQGLELGA
                     DDYITKPFDVQELRLRVRNALKRVSQGSLTNPVTGLPEGALVDEKLSEVLGTDNSSLL
                     FIS"
BASE COUNT          899 a          814 c          972 g         1005 t
ORIGIN      
        1 gatcggctga gctgtgccgg gcacacggat gatgtagtta ccgttcgcgg cgatgccagc
       61 ttcgagttgc tcgccgggtt tgaccacgtg gttattaagg ttggtctgca atttggcggt
      121 gaagggaaaa aaggcgggct gtgaattgtt ggtagcgggt ttctcagttt ttttccacgg
      181 caggatgttg aagcgcggca aacgcagttt ggtaggggag gatgatttgg gtgctgctgg
      241 tgaagatgcg ctttcgttct tgccgccggt caagcccagt ttggaagcag tcccgtaatt
      301 ttttgtgaac tcggttttcc actcgctggc gatcgagggt gccatggttc gggcgaggtc
      361 gtcggtgttt ttctcgatct ctgccaagga tttgaagaac aagatatcct tctgtgcgcc
      421 ttccgccatt tgacggatga tctccatgtt gccgaggtga taggcgatac cagccagaat
      481 attgagggcg atcaggatcg acataccgat cagaaccgta cgggcctcgg attgtgtcaa
      541 ctctttcaca actccatttg cacctgcctg ttgcaaagtg tccatcgtga acagggcagc
      601 ttcgcccaga aaatccacca agaccataag caaggcaatg cctttttgaa caatcccatc
      661 agctttgaac aggaagatgg tcagccaacc gatcatgccg ccagatgttg cgagtaaggc
      721 aaacatgcca aggatttgat tatcttcatc cagcgacgct tgaatgaaat gaagcgagcg
      781 actggctgcg tacaccagca gaccgattga gatcgtatag aacgcgagtt gagcaaaaaa
      841 gcggccaaag gctttcatga ttatttacct ccacaatagg ttgcgttgac ccagccggtt
      901 ttcccgttgc tggtttggac gttgatccag gttgtgcctg tttgaaggat ggtgagggat
      961 tcgccttgca ggagccacgc atctaccggg cagtttgtcc cggcgcaaga gcgtagatgg
     1021 aggacagttg cactgacggt gcaagaagtt tgtgaagaga tagctgacag atcaagagtc
     1081 tgttttgctt cgatgggatt tgcatagtca cgtgcgatgg gggaggaggt gggcgtcact
     1141 gatggacgtg aggagctatc agtcagcgaa catgcaaggg aaattgcgca gagagatatt
     1201 gcccataaag ctaatgaacg atattttatg gatgggtatt ttgagggttt aattttcatt
     1261 gtgtctccta gttgaaaaaa ttcgttatgt tagaatagga gacgcagata ttttctgtat
     1321 gggcgctgtt gtaccggcta ggtatgtaaa cagcgcccgc tcttatctgc gtcatggtag
     1381 ggtagtggga agattagcca gcatcacctc cttgcaatag agatttattt tgttcaaaac
     1441 gaaagttcga aatgaaataa tgaagatgtg atgttattga ttcggcaatg atcacttggg
     1501 cttaggctga gcaataagat ctgcataccc tgctgatttg agatactcaa taactgcacg
     1561 agtagggatt cgataggtac gtttgccaaa tttgatggct ggtaacttct tcgcttcgat
     1621 ccagcgatat acagtttttg tgctaactcg tgcccatcta gctacctgag cgaccgtgag
     1681 gaattccacg ctgagtatgg cttcttcgtc tgagggtatt tttccggctg tcatagaatc
     1741 tccctggagc cggtgaaaag gtaaaacaag aaaccccggc tccgatgaat ttcttcatca
     1801 gaaaccgggg tttctttgcg cagagttacg ctctgcggtc ccgacgggat tcgaacccgc
     1861 gatctcggcc ttgacagggc cgcatgttag gccgctacac cacgagacca attagttaag
     1921 cgggcgatag tttaccatga gggtatattc gtgtcaatac gaactttgga aattcgttat
     1981 gcaatttcaa aagacgaatc gcataaatta ttattcgatt gtgtattgtg ataatgcctt
     2041 gcccttttgc aaactacaca acaacgaact tgacacaatg attgccatgt tctaaaattg
     2101 ttctgttgtt tgtaaattga cgtcctaaga ttcttggggc gtaattattc cccccaggag
     2161 ggctggcttg tctaaaaatc gcacacagca aatggtggac cgcctgcgcg aattacaggc
     2221 atcttcgccg gatatcgaag cgtctgcaat cgttagcgtg gatggtttga gcattgcatc
     2281 tgccctgccg caaggcgtgg aagaagatcg tgtttccgcc atgtctgcag caatgttgtc
     2341 attaggtgaa cgcatcgccg gtgaattggg acgtggctcc cttgagcaag tgtatatcaa
     2401 gggtttgaaa ggctacgttg ttttgatttc cgtcggtgaa gaagcggttc ttaccgccct
     2461 cgtacgtgaa caggccaggc ttggcctgat cttccttgat atgcgacgcg ccgctgaaga
     2521 tctggcgaag ttgatttagt ggagtcatta tgagttccgt acagaaatct ggtttgtatt
     2581 accccaataa gtttggtttg atcacaatta aatcgctcga agaagtgatg ggtaagaatg
     2641 gtttaaatgc tattcttaac ctcggtggct taagtcacta cattgaaaac tatccccctg
     2701 ataacctcga taaaggtttt gactttacag aactttctgc catcggttct tcactcgaag
     2761 aaatgtatgg tcctcgtggc ggacgtggtt tggcacttcg tgctgggcgt gcgacttttg
     2821 ccgatgccct tcgcaatttt ggcgctttgg ctggtgccgc agacttggca tttgttgtcc
     2881 ttccgcttca atccaagctc agaattggtc tgccagcctt tgcaaaaatc ttcacccagc
     2941 ttagcgacca acaaaccacc gttgaagaaa aagatactga atgggtctgg accatccaca
     3001 agtgcccctg ctgttggggg cgcactggcg tggataagcc agtctgtttt attgctaccg
     3061 gtctgcttca agaaagcctc aagtgggttt ccggtggaaa cgaattccgc gtaaacgaat
     3121 ccaagtgtat cgcgatgggt gacccggtct gcgaattcat cgttcaaaaa gaaccgatct
     3181 cctagccaca aggcattgag catggcagac gcaaagtcga agatcctcat agtagaagac
     3241 gacccagatg tggccgaaat gttgaccgcc tattttcgga ctcaggatta cgaagtattt
     3301 accgtaaatt ggggtgaaga tggcgtccgc tctgcattgc aggtcaaccc tgatctcgcc
     3361 atattagata tccgtttgcc agatattgat ggctatgaag ttgcccgccg ccttcgtact
     3421 gatcgtcgaa ccgcccaaat tccaattatt ttcttaaccg aaaaaagaga ccgtgcagac
     3481 cgcttgcaag ggttggaact tggtgctgat gattacatca ccaaaccttt tgatgtgcag
     3541 gaattacgtc tgcgtgtaag aaatgctttg aaacgcgtca gccagggttc tttgactaat
     3601 cctgtaacgg gcttacccga aggcgcactc gtagatgaaa agttatctga agtccttgga
     3661 acagataatt catcgctttt attcatctcc
//
LOCUS       DAOQFT010000220         3689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14116_length_3689_cov_5.863511, whole genome shotgun sequence.
ACCESSION   DAOQFT010000220 DAOQFT010000000
VERSION     DAOQFT010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14116_length_3689_cov_5.863511"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLR93_06700"
     CDS             complement(<1..832)
                     /locus_tag="PLR93_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26691.1"
                     /translation="MDNLENKSSGGMTSIIVGIVVLLCCLCLLAAGIGGYAYYTFMQS
                     GSTGPIEVFGDPISPDNNPNQPTEPTELVRPSVDSISSETVDTLEKAIVPENDPRVLA
                     CRLDGKCGVPEVMATSASPRSVGEQQNFWVHDLDTNENNEVQATLRYITPHVYFWVQD
                     GLDIDEGEMKKLVDTFENQMYPTNREFFGSEWSPGIDGDEHIYILYSRGLGFSVAGYF
                     SSSDSTHPMIQEYSNAHEMFLFNADNTPLGGDYAYAVLAHEFQHMIHWNNDLNETSWL
                     N"
     gene            978..1613
                     /locus_tag="PLR93_06705"
     CDS             978..1613
                     /locus_tag="PLR93_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH26692.1"
                     /translation="MKKTQPTSPRVQRNREVATSAILDTARAIMREEGVAALSMHELA
                     RRMGMRAPSLYNYFNSKMEIYDALFCLGFNLYNTQLQEALKESKTPHDEIRIAIECYM
                     SFALENPELYQLCFERPVPGFVPSKESLELSFGILNNTYTRIERFKKQFDTKLTAQQI
                     VDMVIAVAHGLTALHLANEPQLPIGQGRFGSLIPTFVRFFETSMPTKGETK"
     gene            1610..2434
                     /locus_tag="PLR93_06710"
     CDS             1610..2434
                     /locus_tag="PLR93_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HNH26693.1"
                     /translation="MTAILSTSNIARAEAIPMTHADEAHRLMTEALERLLSLLESLHA
                     DDWSKPTACTAWNVHDMVAHQAGGYLSGISLREMVRQYSQVPKKGQLPEDAINELQVG
                     ERKNKSDSELIAELKQVGAIAIHNWAYGFNAIKWIAMPHPVGGFMSIRHLMWVIHSRD
                     TWMHRLDICRATNRPFEQTREHDGRIVELVVLDTAKKLKKRLNGHAINLMLTGVAGGT
                     WQIGNSSPIAELEMDALDFNIFVSGRYSFEEGMQRAKISGDTKLVESAFKNFLILY"
     gene            complement(2485..>3689)
                     /locus_tag="PLR93_06715"
     CDS             complement(2485..>3689)
                     /locus_tag="PLR93_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH26694.1"
                     /translation="KLAAEEEQAGLERDYERAAQKKAERLRLEQEYGGKRDQWEAEHH
                     LDEVVDVNDIAAVVHQWTGIPVTQMLETESEKLLHMEARLHERIIGQEEAIHAVSDAI
                     RRARSGLKDPARPIGSFIFIGPSGVGKTELAKALAWFMFDDEDALVRVDMSEYREQHT
                     VSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVVLFDEIEKAHPEVWNALLQILDDGRLT
                     DGQGNVVDFRNTVLIMTSNLGTEYVRKGGTLGFLPGKADSSDREAHDKIDKALKAAFR
                     PEFLNRIDEIIMFSPLSVEEMEHIVILQMKEVQDRLNDHNITVQLTDAARSWLAKEGY
                     DPAFGARPLRRAIQKYVESPLSVELLAGKFKDGAEVVVDVDAQENKIVFSTSAPTKKK
                     KSKQEVDA"
BASE COUNT          974 a          950 c          827 g          938 t
ORIGIN      
        1 cgttcaacca ggaggtttcg ttcaagtcat tgttccagtg gatcatgtgc tggaactcat
       61 gcgccaaaac cgcataagca taatcgccgc caagcggagt attatctgca ttgaacagga
      121 acatctcatg tgcattcgaa tattcctgga tcatggggtg tgtggaatcg gaagatgaga
      181 agtaaccagc aaccgagaaa ccgagcccac gagagtacag gatgtaaatg tgctcatcgc
      241 cgtcaatacc cggcgaccat tcactgccga agaattcacg gttggtgggg tacatttggt
      301 tttcgaaggt atccaccaac tttttcattt cgccttcgtc aatatccaga ccatcttgca
      361 cccagaaata gacatgcggc gtgatgtaac gcaaggttgc ttgaacctcg ttattctcat
      421 tcgtatcgag gtcatgtacc cagaagtttt gctgttcccc caccgaacgc ggagatgccg
      481 aagttgccat aacttcaggg acgccacatt tcccatctaa acgacacgcc aagacacgcg
      541 ggtcattctc agggacaatg gctttttcca atgtatcaac agtttcagat gagatcgaat
      601 ccactgatgg gcgaaccagt tcagtcggtt ccgttggctg attcggattg ttatctgggg
      661 aaatagggtc gccaaaaact tcaattggtc cagtgctacc agactgcata aatgtgtaat
      721 aggcatatcc gccaatccct gccgccaaaa gacagagaca gcataacagc acaacgattc
      781 ctacgatgat gcttgtcatg ccgccagatg atttattttc aagattatcc atgaatgttc
      841 ctctctccta aaaggttgtc cgatgatacc gccatccgct aaaaaacgga ttaggacttt
      901 aggggtaatg gtgagccaat ctcttgacaa aggcaatttt tctaatataa ttagaaaaaa
      961 ctaacaatat tagaaaaatg aagaaaacac agcccacctc cccccgtgtt caacggaacc
     1021 gtgaagtcgc aacctctgct attttggata cggcccgcgc tatcatgcgt gaagaagggg
     1081 ttgctgccct ctccatgcat gagttggcgc gtcgtatggg catgcgcgct ccgtcactct
     1141 acaactactt caacagcaaa atggaaatct atgatgccct cttctgtttg gggtttaacc
     1201 tgtataacac tcaacttcaa gaagcgctca aagaatcaaa gactccacat gacgaaattc
     1261 ggattgccat cgaatgttac atgtctttcg ccttagaaaa ccctgagctg taccaactat
     1321 gttttgagcg cccggttccc ggttttgtgc cctccaaaga aagcctcgaa ctcagttttg
     1381 gcattcttaa caacacctat acacgcatag aaagattcaa gaaacaattc gatacaaaac
     1441 tcaccgctca acagatcgtg gatatggtca ttgcagtcgc tcacggtctc acagctttgc
     1501 atcttgccaa cgaaccgcaa cttcctattg ggcaaggacg cttcggttct cttatcccta
     1561 ctttcgtacg tttctttgaa acgtctatgc ccaccaaagg agaaaccaaa tgaccgccat
     1621 cctttcgaca tcaaacatcg cccgcgctga agccatcccc atgacccatg cggatgaagc
     1681 ccatcggctc atgaccgaag ccttggagcg gcttttgagc ctgctcgagt ctctccacgc
     1741 tgacgattgg tctaaaccta cagcctgcac agcatggaat gttcacgaca tggtagcaca
     1801 ccaggcaggt ggatacttaa gtggcataag tttacgagag atggttaggc aatattccca
     1861 agttcccaag aaaggacaat tgcccgaaga tgccattaat gagttacagg tgggagaacg
     1921 caagaataaa tctgattctg aattgatcgc tgaactaaaa caagtcggtg ctattgccat
     1981 tcataactgg gcatatggtt tcaatgctat caaatggatc gccatgccgc acccagttgg
     2041 cggtttcatg tcgattcgtc acctaatgtg ggtcattcac agccgtgata catggatgca
     2101 tcgtctggat atctgccgcg ctaccaaccg tccttttgag caaacgcgtg aacatgatgg
     2161 gcgtattgtg gagcttgtcg ttttagatac agcgaaaaaa ctaaaaaaac gtctcaatgg
     2221 tcatgccatc aatttaatgc tgacaggtgt ggcaggcgga acctggcaga tcgggaatag
     2281 ctccccgatt gcagagcttg aaatggatgc actggacttc aacatattcg tctccggtcg
     2341 gtattcattt gaagaaggta tgcaacgtgc taaaatttca ggcgacacaa aactagttga
     2401 gtccgctttc aaaaatttct tgatcttgta ttaataaata ctacaataaa aaagtccctc
     2461 gaatttattt cgagggactt tttgttatgc atctacttct tgcttcgatt tctttttctt
     2521 tgtcggagcc gatgtactga agacaatctt attctcctgt gcatcaacat ccacaactac
     2581 ttcggcgcca tctttgaact taccagccag caactccact gaaagcggac tttccacata
     2641 cttctggatt gcccggcgca atggacgtgc accaaacgcc ggatcgtaac cttccttcgc
     2701 cagccacgaa cgagccgcat cggtcagctg aactgtgatg ttatggtcgt tgaggcgatc
     2761 ttgcacttcc ttcatctgca agatcacaat atgctccatc tcttccacag agagcggtga
     2821 gaacataatg atctcatcaa tgcggttaag gaactcaggg cggaaggcag ccttaagcgc
     2881 cttgtcgatc ttgtcatgtg cttcgcggtc agagctatcg gctttgcccg gtaaaaagcc
     2941 caaggtgccg ccttttctca catattcagt acccaggttc gaggtcatga tgagaaccgt
     3001 gttacggaaa tccaccacgt tgccttgacc atcggttaag cgtccatcat ccaaaatttg
     3061 gagcaatgca ttccacactt cagggtgggc tttttcgatt tcatcgaaga gcaccacgcg
     3121 atagggtctg cggcggacgg cctctgtgag ttgaccacct tcttcgtagc caacgtatcc
     3181 gggaggcgcg ccgaagagac gtgagacggt atgctgctca cgatactcag acatgtccac
     3241 acgcacaagc gcatcttcat catcgaacat gaaccaggcg agagctttgg caagttcggt
     3301 cttccccaca ccagaaggtc cgataaagat aaacgaacca atcgggcgag ccgggtcttt
     3361 caacccagaa cgcgcgcggc gaatggcatc agacacagca tgaatggctt cttcctgtcc
     3421 aatgatgcgc tcatgcaaac gcgcttccat atgcaagagt ttttctgatt cggtttcaag
     3481 catctgcgtg acaggaatgc ccgtccactg atgaaccacc gcagcaatgt catttacatc
     3541 caccacttca tcaaggtgat gttcggcttc ccattgatcg cgcttgccgc catattcttg
     3601 ctcaaggcgt aagcgttcag ctttcttttg agcggcgcgt tcgtaatcac gttccaaacc
     3661 agcttgttct tcttcggcag ccagtttgt
//
LOCUS       DAOQFT010000221         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14121_length_3688_cov_7.135976, whole genome shotgun sequence.
ACCESSION   DAOQFT010000221 DAOQFT010000000
VERSION     DAOQFT010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14121_length_3688_cov_7.135976"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLR93_06720"
     CDS             <1..301
                     /locus_tag="PLR93_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26695.1"
                     /translation="PDGVIKSIENRRKSENNIESLRQLMAVGSRMDARTFNLKYLETL
                     EKIGEGKSTKYIIPMEFFNLLQDFIRGQNNNGANTAGNNGNGNNPPEQLPGGPIQ"
     gene            complement(317..547)
                     /locus_tag="PLR93_06725"
     CDS             complement(317..547)
                     /locus_tag="PLR93_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26696.1"
                     /translation="MSNVSDIKPWFREDIARTLMSIYFASAANNSQRTTENENYRVGF
                     AAALSSVALAVGVNPESFLAPEDIQRLRSTSR"
     gene            847..2265
                     /locus_tag="PLR93_06730"
     CDS             847..2265
                     /locus_tag="PLR93_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26697.1"
                     /translation="MKSAFSGIGSFLQTPFGVIAAGTVVFHGILFAVGFNYTVVTLVI
                     AVVVLGVNLFAANYIADKLVYFYSQFVLPVQTPKDRQEIYTRVSGFDGSRGPILFVKN
                     GRVIKHEGETEKKGPGVIVLDTASAIVTQTETEIIGAAGPGIHFTKGREKITRGEGVD
                     LRPQWQFIGPLASDQPFLNPPSPDSKKYNELQARRQQTAGQTRDGFEISPTISIKFRI
                     KQPEEKTPSESGVVSHYGYNATAVLHAVTREVIELGTSDNKRTRMEWYRLPAHLVVNL
                     WREYVRKFKLEDLFKAEGVSGLQTIEEMINRRVKRPEVVAMDDTGIPTGEALPSLEYQ
                     QLIERGLEVIDVRIHNVLFDPAIEEQTIKNWNNEWTKIAKREEDLLNEREKLIETAAH
                     NEAVKRFARLASLKFENPIAMPADAYTTLQALIEPVKETLVIESRANSQMETDIKKLD
                     EIWKWLLVSKLDKSMHRKEDES"
     gene            2262..>3688
                     /locus_tag="PLR93_06735"
     CDS             2262..>3688
                     /locus_tag="PLR93_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26698.1"
                     /translation="MNRFRRRRDEMIPRIFALTDEAVEYRSQIRNYARIVVWAVLSGF
                     TLILFFFFAETTIQRLTVLGLSLLIYLPLFSAAQTVARHKAANYLADIYEIEDETIAD
                     SFLEEVIFGEGTEKITINEGKISERDEESPLIMIGGPGYIQVNLDSVALLERVDGTPE
                     IIHPRGKPWKLGSFERIREIGKHDEPGKREYAIINLRDQFVRGLSVRSRTKDGIPLEA
                     QDIKVLFSILRKPKEEAPENDPYHFDETAVHSLVYNQTIISPSPAKTAGVSFPWDTTV
                     IPLITSEMENLITSRNLSEILASISHKEIDDLNEGEATNKQIRLEMTGEQTVPTNSNS
                     PSAPNFESRSKITAQFFSPQFKAKAAKLGVSIHWIDIGTWQLPSEMILEELKSGWKMM
                     RENVRRRNDVNRLAKRQEMKELRELVNHVIVSSFTKANNANDNRKLSDDEFSKLSKII
                     EENPNIAFSPALQQKMAQDVTLKKDA"
BASE COUNT         1065 a          863 c          869 g          891 t
ORIGIN      
        1 gccagatggc gttattaagt ctattgagaa ccgccgtaaa tctgagaata atattgaatc
       61 tttaagacag ttgatggcag tcggttcccg catggatgct cgcactttta accttaagta
      121 tcttgaaacc cttgaaaaaa taggtgaggg aaagtcaacg aagtatatta tccctatgga
      181 attctttaac ttattacaag acttcattcg agggcaaaac aacaatggcg caaacactgc
      241 aggaaacaac ggaaacggaa ataatccacc tgaacaattg cccggtggac ctattcaata
      301 atctataaac aataatttat ctggatgtgc ttctaagtct ttggatatct tcgggagcga
      361 ggaagctttc gggattcacc cccactgcta atgccacgct ggaaagcgct gcggcaaaac
      421 ccacgcgata attttcattt tctgtggtgc gctgtgaatt atttgcagcg gacgcgaaat
      481 atatgctcat aagagttcgg gctatatctt cacgaaacca tggtttgata tcacttacat
      541 tagacattgt cttactcctt ggcgttacat aaatgcaact aaataaatta tagaacatga
      601 gttctattta aacaagagcg agggtatttt acacctgtaa gggagaagta gagcgtttac
      661 agatactttt catagcttaa cacttattca aggtagttcg atgtgtctcc cagtgcgcaa
      721 aacgcgggaa atatatcaca aagaatcacc atgaaagggg tccattcggg ggattcgaaa
      781 attcaaccta tcctgatata tttcaacccg ccagattggc aaattttgat ttagaatcat
      841 gcgacaatga agagcgcctt tagcggaatc ggttcatttt tacagacgcc ttttggggtt
      901 atagcagcag ggacggtcgt ctttcatggc attttgtttg cggtcggttt taactacacc
      961 gttgtcaccc ttgtaatcgc ggttgtggtc ttgggcgtaa acctttttgc cgcgaactat
     1021 atcgccgata aactggtcta tttttattct caatttgtgc tccccgttca aacaccaaaa
     1081 gaccggcagg aaatctacac ccgtgtcagc ggatttgacg gcagccgtgg accgatcctc
     1141 ttcgtcaaga atggacgggt gatcaagcac gaaggcgaga ccgagaaaaa aggtccgggt
     1201 gtgatcgtgc tcgataccgc cagcgccatt gtcacccaaa ccgaaacaga aatcatcggc
     1261 gcggcaggtc ccggcatcca ctttacgaag ggacgtgaaa agattacccg cggtgaaggc
     1321 gtagatttgc gcccgcaatg gcagtttatt ggtccgttgg cgagtgacca gccgtttctc
     1381 aacccgcctt cgcctgattc gaaaaaatac aacgagttgc aggctcggcg tcagcagacc
     1441 gccgggcaaa cgcgcgatgg gtttgaaata tcccccacta tcagtatcaa gttccgtatt
     1501 aagcagccag aagaaaagac tccttctgaa agcggtgtcg tttctcatta tggttataat
     1561 gccactgccg tcctccatgc ggtgacacgc gaagtcattg aattgggaac ttcggataac
     1621 aaacgtactc gcatggaatg gtatcgcctt ccggctcacc ttgtggtgaa tttgtggcgc
     1681 gagtatgtgc gcaagttcaa attggaagat ttgttcaaag ccgaaggcgt cagcgggtta
     1741 caaaccatcg aagaaatgat caatcgtcgg gtgaaaagac ccgaagtagt cgccatggac
     1801 gataccggaa ttccaactgg tgaagcattg cccagtctcg aatatcagca actcatcgaa
     1861 cgcggtcttg aggtgatcga tgtgcggatt cacaatgtgt tgttcgaccc tgccattgaa
     1921 gaacagacca tcaagaattg gaacaacgag tggaccaaga tcgccaagcg tgaagaagat
     1981 ttattgaatg aacgcgagaa gttgatcgag acggcggccc ataatgaggc tgtcaaacgt
     2041 tttgcgcggc tcgcttcgct aaagttcgag aatccgatcg ctatgccggc tgatgcatac
     2101 acaactctgc aagctctgat cgaaccggtt aaagagacct tggttattga aagccgcgcc
     2161 aacagtcaga tggaaactga catcaagaaa ttagatgaaa tttggaagtg gttgttggta
     2221 agcaagttgg ataaatccat gcatcgcaaa gaggacgaat catgaatcga ttccgccgtc
     2281 gacgcgatga aatgattccg cgcatctttg ctctcaccga tgaggctgtc gagtatcgaa
     2341 gccagattcg caactatgcc cgcattgtgg tgtgggcagt attaagcgga ttcacactaa
     2401 tcttgttctt tttctttgcc gaaactacga tacaacgact gacggtgttg ggcttaagtc
     2461 tcttgattta cctgcctttg tttagtgcag cgcaaaccgt tgcaagacat aaagccgcca
     2521 attatctggc agatatttat gaaatagaag atgaaacgat cgccgatagt tttcttgaag
     2581 aagtgatctt cggcgaaggg acagagaaaa tcacgatcaa cgagggcaaa atctcagaaa
     2641 gagatgaaga gtcgcccctc atcatgatcg gcggacccgg ctatattcag gtcaacttgg
     2701 atagtgtggc gctgctggaa cgcgtagatg gcactcctga gatcattcac ccacgcggca
     2761 agccgtggaa gctgggcagt tttgagcgca tccgtgagat cggtaaacac gatgagcccg
     2821 gcaaacgaga atatgccatc atcaacctgc gcgaccaatt cgtgcgcgga ctttccgtcc
     2881 gctcgcgcac caaagacggt attccgcttg aagcgcaaga tatcaaagtg cttttcagca
     2941 ttttgcgcaa gcccaaagaa gaagcgcctg aaaatgaccc gtatcatttc gatgaaaccg
     3001 cggtccattc cttggtgtat aaccaaacta tcatttcacc ttcgcctgca aaaaccgctg
     3061 gcgtttcttt cccatgggat acgaccgtta ttccgctcat cacaagcgaa atggaaaacc
     3121 tcatcacttc gcgcaacttg agcgaaattc ttgccagtat cagccacaaa gaaattgatg
     3181 acctcaacga aggtgaagca accaacaaac aaattcgtct cgaaatgacg ggcgagcaaa
     3241 cggttccaac caacagcaat tctcccagtg caccaaactt tgagtcacgt tcaaaaatca
     3301 cagcgcaatt tttcagtccg cagttcaaag ccaaagccgc caaactcggc gtcagcattc
     3361 attggatcga cattggcaca tggcagcttc cttctgaaat gattctcgaa gaattaaaaa
     3421 gcggttggaa gatgatgcgt gaaaatgtcc gccggcgcaa cgatgtcaac cgacttgcga
     3481 aacggcagga aatgaaagag ttgcgcgaac tggtaaatca tgtcattgtt tccagtttca
     3541 ccaaagcaaa caacgccaac gacaatcgca aactttcaga cgatgaattt tcaaagttat
     3601 ccaagatcat cgaggaaaat ccgaacatcg ccttcagccc tgccttgcag caaaagatgg
     3661 cacaagatgt caccctcaaa aaagatgc
//
LOCUS       DAOQFT010000222         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14171_length_3680_cov_6.473379, whole genome shotgun sequence.
ACCESSION   DAOQFT010000222 DAOQFT010000000
VERSION     DAOQFT010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14171_length_3680_cov_6.473379"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..222
                     /locus_tag="PLR93_06740"
     tRNA            150..222
                     /locus_tag="PLR93_06740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:182..184,aa:Gly,seq:tcc)
     gene            303..375
                     /locus_tag="PLR93_06745"
     tRNA            303..375
                     /locus_tag="PLR93_06745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:336..338,aa:Ile,seq:cat)
     gene            complement(430..1203)
                     /locus_tag="PLR93_06750"
     CDS             complement(430..1203)
                     /locus_tag="PLR93_06750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNH26699.1"
                     /translation="MKPTLSDLERLARGAGAILRDGYNKEHTVHYKGVIDLVTESDHA
                     SEAFLLGEIKSAFPESNILAEESGNIQGSNEDLWYIDPLDGTVNYAHHIPIFCVSIGF
                     STNGVLTLGAVYDPMRDEMFLAEKGKGATLNGKPIRVSNTDELEKSLLVTGFPYDTWN
                     TELDNFKYFERLAKKTQGVRRLGSAALDACYVGAGRFDGFWEYKLKPWDVAAGGLIAA
                     EAGAKVTSIDGKENFLAATPLSILATTPGIYEATKEQLK"
     gene            complement(1203..2105)
                     /gene="rocF"
                     /locus_tag="PLR93_06755"
     CDS             complement(1203..2105)
                     /gene="rocF"
                     /locus_tag="PLR93_06755"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNH26700.1"
                     /translation="MQIDIIGVPIDLGADRRGVDMGPSAIRYSLLQKKLEDLGYTVND
                     EGNVEVPIAEMCKITNPKLKYIDCIIPMSRRVSGAVSTSIQAGNFPLVIGGDHSLSIG
                     SVRGAARNRKIGVIWVDAHADFNTAETTPSGNIHGMSLAALCGMGDPSLVQLWDEPVP
                     VIDPKKIAIVGARDLDEGEKRNLREAGAMVMGMEQVDRYGMVACLEKAIERISRDVDG
                     IYLSLDLDALDPEHAPGVGTPVPAGLTQREAHLACEMIGETGKLIGMDVVEVNPILDI
                     QNRTALLAVDFALSALGRRIWNGI"
     gene            complement(2142..>3680)
                     /locus_tag="PLR93_06760"
     CDS             complement(2142..>3680)
                     /locus_tag="PLR93_06760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH26701.1"
                     /translation="TLMFISLAFWSFGYAMITLSPQLKDKIFWLKFENFGIQTTALFW
                     FLFAMMYTKMDSWMDNKIVLLALWVPPAVALTFILSDRWFHLYYSSVKIATEDGGPLL
                     IERGPFYIYALVCTYTLYLAGLAALVRRFLVSRDIYRSQTPYLIGAVIIPFSLNIIYQ
                     VNPGILPIFPAPVDLTALAFTFTAAFLATSIFGLRLLDLVPIARDNVIESIPEMVFVL
                     DDKERIIDANAVAQQWLEKPFAEILGKEPIEVFQKWPQLLQRFFFMERTREEIEIPGD
                     PPRTLETIITPIYNRTNQLEGRVILAYDITDRKKLEKDLKDTNESLQAQLQENERLRV
                     QLQEQAIRDPLTGVFNRRYLAEALDKETARAVREKSPFSLIVLDVDFFKKVNDTYGHK
                     CGDLVLQSLAKFLQENTRRSDIVCRFGGEEFVILMPDAAVEDAYERAERFRNNFALQV
                     VEHEGKQVKSTFSAGVASFPVHANVGETLLSMADLALYQSKANGRNRVTVFSPEIEEP
                     APAK"
BASE COUNT          961 a          827 c          904 g          988 t
ORIGIN      
        1 tgcgacctat tgctccgcaa gcaagcgctc taatccactg agctacggaa gcgttcattt
       61 gttaagcggg cagtatttta ctgcattgta ttctgaaggt caaggtgctt gacggtacgc
      121 tccccagcat gtaaaatcag cgtcgctttg cgggagtcgc caagtggtaa ggcatcagcc
      181 ttccaagctg acatttcgtg ggttcgaatc ccatctcccg ctctctagaa gtgttccagt
      241 gtcatgtgat gaagtgtcag gttacgtgac acacggcact ggaacatttt cacttaagtc
      301 acgggctcgt ggcgcagcgg ttagcgcagg cgactcataa tcgcttggtc gtaggttcga
      361 atcctaccgg gcccactttc aatttatgat ttacaaaaaa aagtggacgg cgatgagccg
      421 tccacttttt tattttagct gttcttttgt cgcttcataa atgcctggtg tagtagcaag
      481 aatggataat ggagtggcgg caagaaaatt ttctttgcca tcaatcgatg taacttttgc
      541 tccagcttca gcggcgatta agcctccggc ggcgacatcc caaggtttga gtttgtattc
      601 ccagaagcca tcgaagcgac cggcacccac gtaacaggcg tctaatgcgg cggacccaag
      661 acggcgtacg ccttgagtct ttttggcaag gcgttcgaag tatttgaagt tatcgagttc
      721 ggtattccat gtatcatatg gaaagcctgt aacaagcaga ctcttttcaa gctcatctgt
      781 attcgaaacg cggatcggtt ttccgttcaa ggttgcgcct ttgccttttt cggcgaggaa
      841 catttcatca cgcatggggt cgtagacggc gccgagagtc agcacgccat tggtggagaa
      901 tccgatagag acgcagaaga tggggatgtg atgagcgtag ttgacggtgc catcgagcgg
      961 gtcgatatac cagaggtctt cattgctgcc ttgaatattt ccgctttctt cggcgaggat
     1021 gttactttcg gggaaagcgg attttatttc accaagcaga aaggcttcag aggcatgatc
     1081 agattcagta accagatcaa ttaccccttt gtaatgaacc gtgtgttctt tgttgtatcc
     1141 atcgcggaga atggctccgg ctccgcgggc gagtcgttca agatcagaga gagtaggttt
     1201 catcaaattc cattccaaat gcggcgacca agagcagaga gggcaaaatc cacagcgagc
     1261 aaggcggtgc gattttgaat atcgaggatg ggattaactt caacaacatc catgccgatg
     1321 agctttccgg tttcgccgat catttcacat gctaaatgcg cttcacgttg tgtgagaccc
     1381 gcagggacgg gtgtacccac accgggcgcg tgttcggggt cgagcgcatc aaggtcgaga
     1441 gagaggtaaa ttccatctac atcgcggctg atgcgttcga ttgctttttc aagacaagcc
     1501 accatgccgt accgatcgac ctgttccata cccatcacca tggcacccgc ttcacgtaaa
     1561 tttcgttttt cgccttcgtc gaggtcgcgt gcaccaacga tggcgatctt tttggggtca
     1621 atcactggaa caggttcatc ccataactgt acgaggcttg ggtcgcccat gccacataga
     1681 gctgctaaag acatgccgtg gatatttccg gatggagtgg tttcagctgt attgaaatcg
     1741 gcgtgtgcat cgacccagat gacgccgatc ttgcgattac gtgctgcgcc gcgtacagag
     1801 ccaatggata aagagtgatc gcctccaatg actaagggaa agttgccagc ctgtatcgaa
     1861 gtggatactg cgccggaaac gcgtctcgac atggggataa tgcagtcgat atatttcaac
     1921 tttgggttgg tgatcttaca catttcggca attggcactt ctacattgcc ttcgtcgttg
     1981 acggtatagc cgaggtcttc caattttttt tgcaacaaag aatatcgaat tgcgctggga
     2041 cccatatcca caccgcggcg atctgcaccc aagtcgatag gcacaccgat aatatcgatt
     2101 tgcatgaaag ttctcctaaa ctaaaatgaa tttgaggaat tttattttgc cggggcgggt
     2161 tcttctattt caggtgaaaa cactgtaacg cgatttcgcc cattggcttt tgattgataa
     2221 agcgctaggt ctgccatgct aagaagggtc tcgcctacat tggcgtgaac cgggaacgat
     2281 gcaactccgg cagagaaggt tgattttacc tgcttccctt cgtgctcaac gacttgcaag
     2341 gcaaaattgt ttctaaaacg ttcagcacgt tcgtatgcat cttctacggc agcatcgggc
     2401 atgagaatta caaattcttc accgccaaag cggcacacaa tgtcactgcg acgtgtattc
     2461 tcttgtaaaa attttgcgag agattgcaga acaaggtcgc cgcatttatg tccgtatgta
     2521 tcgttaactt ttttgaagaa gtctacatcg agaacgatca gtgaaaatgg gcttttctcg
     2581 cgaaccgcac gcgcggtttc tttgtctaac gcttcagcta aatagcggcg attgaaaacg
     2641 ccggtcaacg gatcgcgaat agcttgttct tgaagctgaa cacgaaggcg ttcattttct
     2701 tgcaattgag cctgcagaga ttcattcgta tcttttaaat ctttttctag tttttttcgg
     2761 tcagtgatgt cgtatgcaag aatgactcga ccttcgagtt ggtttgtacg gttataaatg
     2821 ggggtgataa tggtttcaag tgtgcggggc gggtcgccgg gaatttcgat ctcttcacgt
     2881 gtacgttcca taaagaaaaa ccgctgaagt aattggggcc atttctgaaa gacttcaatt
     2941 ggttcttttc ctagaatctc tgcaaaaggt ttttccaacc attgttgagc cacggcattt
     3001 gcgtcaatga ttctttcttt gtcatctaaa acgaatacca tttcaggtat agattcaatc
     3061 acgttatcgc gtgcaattgg cacaaggtct agcagacgta gaccaaaaat actggtagca
     3121 agaaatgccg ctgtgaatgt gaatgctaaa gcggttaagt ctacaggagc aggaaagatt
     3181 ggcaaaatac caggatttac ttgataaata atattaagag aaaatggaat aataacggcg
     3241 ccaattaaat aaggtgtttg gcttcggtat atatctcgag agactaaaaa acgtcgcacc
     3301 agtgctgcca accctgcaag ataaagcgtg tatgtacata ccaaggcgta aatgtagaaa
     3361 ggaccacgct ctataagaag aggtccacca tcttcagtag cgattttcac gctgctgtaa
     3421 tacaagtgga accatcggtc agacaggata aaagttaatg ctacagctgg gggcacccac
     3481 aatgccaaga gtacaatctt gttatccatc caactatcca ttttggtgta catcattgca
     3541 aacaagaacc aaaacagtgc agtggtttga atgccaaaat tttcaaattt gagccaaaaa
     3601 atcttgtctt tcagttgtgg cgataaagtg atcatggcat acccaaaaga ccaaaatgcc
     3661 agcgaaatga acatcaaggt
//
LOCUS       DAOQFT010000223         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14181_length_3679_cov_7.595475, whole genome shotgun sequence.
ACCESSION   DAOQFT010000223 DAOQFT010000000
VERSION     DAOQFT010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14181_length_3679_cov_7.595475"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2336
                     /locus_tag="PLR93_06765"
     CDS             <1..2336
                     /locus_tag="PLR93_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH26702.1"
                     /translation="GRIISRFGLRAGLLATPTLTLLFTAALAAAGTLKPEMLPAIFWL
                     AVTGKFTNEGLGFSLDQTADNLLYQPIHERIRARAQTVTEGIIQPLAIGLAGGMLLLF
                     NTALKFNAIQLAYIYIAFAIIWIIICIALIRAYPVALTEALHKRRFGDKGILLTDASS
                     IQIVRMALKSQHASEVLYALDLLEKGESDTFIDDLETQIRSPMPEVRKDVIQRIERLK
                     ITKLLRDIQIQLRTETDPNVREIASRALIVLGADSSTALSLLAQEDAATQRGAMIGAL
                     RILREPKDSNAYERLLELTKSKQVQDRVYAAQLMAEAANPILSNVLLALLSDEDLQAK
                     KAALRAAAKFQHPDLYSKIIPALGSPLTRSLAFNALVEGGNHAIPSIIEALKDISLHR
                     RIHLRLIRACSHIKSEAAIHVLEGLIHHSNVSVRSRALTALHDCRYKPEGESAFGVSE
                     QVTAESRRAAWLLGVMHDLGKSTKTEVLVRAASHDLDGTIDRLFHLFEFIYEPQAIQR
                     AQHAIQRKDANRKSYAIEVLDTTLSKPHKTAFIPLLEDFAPKETLEKMGSEYKQTFLP
                     PAGRVMDILTNPLAKQNPWLVATAIEFADSMGISLDSEVTDSLSSSNESLLARMVLQK
                     RKEKMMLSTVERVIVLKSLSMFEKTPDEALAELAELVQEMVVQPGDEIIREGETGDSL
                     YIIVDGKVEVVDDNRILNQLGARAVFGELSMLDSSPRTATIRAIEETSLLRLDQTSFY
                     EIMSDYVEVAMGTIQLLTRNLRARTGDVMELSRMLGQ"
     gene            2350..2742
                     /locus_tag="PLR93_06770"
     CDS             2350..2742
                     /locus_tag="PLR93_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26703.1"
                     /translation="METKPALDSKLVEEFVGNSHGNFARVKELLEQEPALVNATWDWG
                     GGDFETALGAAAHMGNKQIANYLLEHGARLDIFAAAMLDKLDVVKAALEAYPEAVNTP
                     GPHGIPLYVHAEAGDAKSVLEYLNSLEG"
     gene            2808..3560
                     /locus_tag="PLR93_06775"
     CDS             2808..3560
                     /locus_tag="PLR93_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26704.1"
                     /translation="MKKRNQVVIGLIAAIIMLACACPVSGLPFGSEEQPTPGLPILPV
                     ATIVDAPATEAPVQPAANVLLSDDFSSPSAEMETYSGDGGSAGTENGVYVVRSTADLW
                     QWGKSNSQFDNAIFEADITMIAGPTNNNAGFGIICRLSERDDTSIDGYMFAISGDGYY
                     TIRHIVSSNMTALVDWTESSVINQGNASNHLRATCNGSDLRLEVNGQEVASAIAPADG
                     PTNGTMAFAAISFETDQPYAEGHFDNLTVSQP"
BASE COUNT         1010 a          871 c          865 g          933 t
ORIGIN      
        1 cggggcgcat catttctcgt ttcggcttgc gtgctggtct cttagccact cctacgctga
       61 cactgctctt cacagcagca ctcgctgccg ctggcacact caaacccgaa atgcttcctg
      121 ctattttctg gctggcagtt acaggcaaat tcaccaatga aggtttgggc ttttcattag
      181 accaaactgc cgataacttg ctctaccaac ccatccacga acggattcgc gcccgtgctc
      241 aaactgtgac cgaagggatt atccaaccat tggcaatcgg gttggcaggc ggcatgcttt
      301 tacttttcaa cacagcgctt aaatttaatg ccatccaact tgcatacatt tacattgcct
      361 tcgccataat ttggatcatt atttgcatag cacttatccg cgcatatcct gtggcactta
      421 cagaagcctt gcacaaacga cgttttggcg acaaagggat tctcctaacc gatgctagca
      481 gtattcagat tgtccgcatg gcattgaaaa gccagcatgc atctgaagtt ctgtatgcgc
      541 ttgacctgtt ggaaaaaggg gaaagtgata cttttataga tgaccttgaa acacaaatcc
      601 gctcgcccat gcctgaagtg cgaaaagatg tcatccagcg catcgaacgt ttgaagatta
      661 ccaaactgct acgagatatt cagattcagc tacgcacaga gactgacccc aatgtacgag
      721 aaatcgcttc acgcgcactc atcgtccttg gcgcagactc aagtactgct ctgagtctcc
      781 tcgcccaaga agatgctgct acccaacgtg gcgccatgat cggcgcctta agaatattga
      841 gagagccaaa agacagcaat gcctatgaac gccttttgga attgacaaaa tctaagcagg
      901 ttcaagatcg agtttatgca gcacagttaa tggctgaagc cgcaaacccc attctttcga
      961 acgtgctttt agccctttta agcgatgaag acttacaagc aaaaaaagct gctttacgcg
     1021 cagccgctaa attccagcac ccagatttat attcaaagat catccctgct ttagggtcgc
     1081 cactgacacg cagccttgct ttcaacgcac ttgtcgaagg cggaaatcat gcaattccat
     1141 ctattattga agctttgaaa gatataagtc tacacaggcg catccacttg cgtttgattc
     1201 gcgcctgctc tcatatcaag agtgaagcag caattcacgt tctggaaggg ttaattcacc
     1261 actcgaatgt aagcgtccgt tcgcgagcgt taaccgcttt gcacgattgc agatacaagc
     1321 ccgagggaga atccgctttt ggggttagtg aacaggtcac ggcggagtcc agacgcgctg
     1381 cttggttgtt gggagtcatg catgatttgg gcaaatccac caaaactgaa gtcttggtgc
     1441 gggcagcgag tcatgatctg gatggcacga tagaccgcct ctttcatctc tttgagttta
     1501 tctatgagcc acaagcgatt caacgtgcac aacatgccat tcagcgaaaa gatgcaaatc
     1561 gtaaatccta tgcgattgaa gtattagata caactctcag caaaccacat aagacagctt
     1621 ttattccatt gctggaagat tttgcgccca aagaaacgct agagaaaatg ggaagtgaat
     1681 acaagcagac cttcctaccg ccagccgggc gcgttatgga tattttgacc aatccgctgg
     1741 caaagcaaaa cccatggctg gttgcaactg cgattgaatt tgcagatagc atgggaattt
     1801 cgttagacag cgaagtgaca gattcattat cttcttctaa cgagtcttta ctagcgcgta
     1861 tggttctgca aaaaagaaag gaaaagatga tgttatccac tgtagaacga gtgattgttt
     1921 taaagtcgtt gagcatgttc gaaaaaacac cagacgaagc tctcgccgag ttggcagagc
     1981 tcgtgcagga aatggttgta caacccggag atgagatcat ccgtgaagga gaaactggcg
     2041 acagtttgta tattatcgtg gacggcaaag tagaagtggt ggatgataac cgcatcttga
     2101 atcaacttgg agcacgggct gtctttggtg agctttccat gctagactct tcaccacgca
     2161 ctgcaaccat ccgcgctata gaagagacat ccctcttgcg ccttgatcaa acgtcgtttt
     2221 atgaaattat gagcgattat gtagaagtag ccatgggcac gattcaatta ttaacccgta
     2281 acttacgtgc ccgcacgggt gatgtaatgg aactaagccg aatgttaggt caatagagaa
     2341 ggagagaaaa tggaaaccaa acctgcttta gactcaaaac ttgttgaaga gtttgttgga
     2401 aattcacatg ggaatttcgc gcgtgtgaaa gagttactcg aacaagagcc tgcgcttgtc
     2461 aatgcaacct gggattgggg cggcggagac tttgaaactg ctcttggtgc tgctgctcac
     2521 atgggcaata agcagatcgc caattaccta cttgagcacg gtgcgcggtt ggatattttt
     2581 gccgctgcca tgctggataa attagatgtg gtcaaagctg cattagaagc atatcccgaa
     2641 gcagtcaaca cgccgggtcc gcacgggatt ccgctttacg tccatgcaga agcaggagac
     2701 gcgaagtctg ttttggagta tctcaattcc cttgagggat agtaccaaag ggttcttgga
     2761 ctgaacaaat tgggtcgata taatttagaa atactttagg agaaaaaatg aaaaagagaa
     2821 atcaagtagt tataggtctg attgccgcga tcatcatgct cgcgtgtgct tgccccgttt
     2881 caggtttgcc ttttggtagt gaagaacaac ccacacctgg gcttcctatt ttgccagttg
     2941 caaccatagt agatgcgcct gccacagaag ccccagtaca acctgctgca aatgttttgc
     3001 tcagtgacga ttttagctcc cccagcgccg aaatggaaac ttattcaggc gatggcggca
     3061 gtgctggaac cgaaaatggt gtgtatgtcg tccgctctac agctgacctg tggcaatggg
     3121 gtaagtccaa ttcgcagttt gataatgcaa tctttgaagc agatataacc atgattgcag
     3181 gtccgaccaa taacaacgct gggtttggca ttatctgccg cttgagcgaa cgtgatgaca
     3241 catccatcga tggctatatg tttgcgatca gtggagatgg ttattacact attcgccata
     3301 ttgtctcaag caacatgact gcactggtag actggaccga atcaagtgtg atcaaccagg
     3361 gcaacgcctc gaatcacctg cgcgccacct gcaatggcag tgacttgaga cttgaagtca
     3421 atggacaaga agtcgcttcc gctatcgcgc cggcagatgg tccaaccaac ggaaccatgg
     3481 ctttcgccgc catctctttc gaaaccgatc agccctatgc tgaaggtcat tttgataatt
     3541 tgacagtcag ccagccgtaa taattagtag aaataaaaaa gacgtgcttg gcacgtcttt
     3601 tttatttcta ctttgcacgc attttctgcc gaagaaatgc tgcctcaaag atacggtcaa
     3661 agaagccttt atacccatc
//
LOCUS       DAOQFT010000224         3670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14222_length_3670_cov_6.961549, whole genome shotgun sequence.
ACCESSION   DAOQFT010000224 DAOQFT010000000
VERSION     DAOQFT010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14222_length_3670_cov_6.961549"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLR93_06780"
     CDS             <1..675
                     /locus_tag="PLR93_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH26705.1"
                     /translation="AKPGYKIAIVGPTGAGKTTMVKLLMRFYDVDSGKILIDGQDIRD
                     FTRKDLRKMFGMVLQDTWLYNGTIMENIRYGRPDATDEEVIAAAQMAHVDHFVHTLPD
                     GYNMVLNEETSNISQGQMQLLTIARAFLADPKILILDEATSSVDTRTEVLIQRAMTSL
                     MKNRTSFVIAHRLSTIRDADLILVMNHGDIVEQGTHKELLAKFGFYHELYMSQFKKKE
                     DETIVA"
     gene            741..2081
                     /locus_tag="PLR93_06785"
     CDS             741..2081
                     /locus_tag="PLR93_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26706.1"
                     /translation="MTETTPTPPEEKLDFKRILPILVIVLVDLMGLSIIIPLLPLYAA
                     RFGAEPIVVGILQATYPLMQFIGAPVLGRLSDRFGRKPVLIASQIGTLIGFIMLGFAN
                     SMVVLFISRIIDGLSGANISTAQAAIADSTNEKTRTQGLGLVGAAFGVGFVLGPILAF
                     IVLAASGQNYQYVAWTAALFSLISILLTTFWFKETVADAADPEASRKRRPFSFGNLRE
                     AMAHRPTIMFLVIVMFFYQIAFGGYEQLFSLFTLNRLGMDARDTSGLFMLAGILIIVI
                     QGGLIGRWSRKKGDRWLALMGIGALAIGLIGTALTPAMPVPWYDKARVLESLAGQGEF
                     RVSIQSINVSLPDEAARGWFGIIWLMIASIPAALGGGTLQPAINSLITKDSDKSEVGG
                     MLGLSAAAYSAANAIAPLFYGALFQWFGAPVPFFAGGAILLALFLFAPRVIKNS"
     gene            complement(2110..2787)
                     /locus_tag="PLR93_06790"
     CDS             complement(2110..2787)
                     /locus_tag="PLR93_06790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNH26707.1"
                     /translation="MPLDVYRINALCFDVDGTLSDTDDLYVQKVSKLFPRFLFKDPEH
                     AARRFVMWIEAPGNALLGLADTLHLDDEMVAVINWLSRHRKQSDKKFLLVPGVDEMLK
                     QLHGRYPMAVVSARDEKGTMRFLEQYDLVKYFDAVITGLSAVHTKPYPDPILLAAQKM
                     NVSPENCLMIGDTTVDIRAGKSAGAQTLGVLCGFGEEPELRHLGADEIVEDTTKLLEI
                     LTINRER"
     gene            2831..>3670
                     /locus_tag="PLR93_06795"
     CDS             2831..>3670
                     /locus_tag="PLR93_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26708.1"
                     /translation="MTEDPNRALLELLVKVSREVATALDLRTVLQRVLYACIQYVGGE
                     RGSIVVMDDLGKPVDATIVYGKQFHDHTTQQLRETVERGLAGWVVQNRKPALVPDTSK
                     DERWLRRADDAAEKSGAKSAICVPLLARERFVGVLTLVHPVPNAFNEQHLELVQAISD
                     QASIAVLNARLYTETQRTARVMTALAEGAATINSSLEMQDVLRRILNQTMQALQVETV
                     ALGLIDSHSNDLVFQAAAGNNSGHIPGLHVPAGKGLVGKVIQAGRGVILPSIKHDPTL
                     VEED"
BASE COUNT          872 a          927 c          942 g          929 t
ORIGIN      
        1 gcaaagcccg ggtacaagat cgcaattgtc ggtccgaccg gagccggaaa gaccactatg
       61 gtcaagttgc tgatgcgctt ctatgacgtg gacagcggca aaatcctgat cgacggtcaa
      121 gatatccgcg actttacccg caaagacttg cgcaaaatgt tcggcatggt gcttcaagat
      181 acctggctct acaacggcac cattatggaa aacatccgct acggacgccc cgatgcgacc
      241 gatgaagaag taatcgctgc cgcgcaaatg gcgcatgtgg atcatttcgt ccacacgctg
      301 cccgatggct acaacatggt cttgaacgaa gagacttcga acatctcaca gggacagatg
      361 cagttgctga cgattgcgcg cgccttcctc gctgacccga agatcctcat ccttgatgaa
      421 gcgaccagct cagtggatac gcgcaccgaa gttctcattc aacgcgcgat gaccagcttg
      481 atgaagaacc gcacctcgtt cgtgattgct catcgcctct ccaccatccg cgatgcagat
      541 ttgatcctcg taatgaatca tggtgatatc gtggagcaag gaacgcacaa agaattgctc
      601 gcaaagttcg gcttctatca cgaactgtac atgagccagt tcaagaaaaa ggaagacgaa
      661 accatcgtag cgtaataaaa tcaacagacc ctaaagtttc cctttagggt ctgttattaa
      721 atcaaattca aggattgtcc atgactgaaa caacaccgac tccccccgaa gaaaaacttg
      781 atttcaaacg catcctgccg attctcgtca tcgtactcgt agacctgatg ggactttcta
      841 ttattatccc tttgttgccg ctttatgccg cgcgcttcgg tgcggaaccg atcgtggtcg
      901 gcattctcca agccacgtac ccgctgatgc agttcatcgg cgctccagtt ctgggtcgcc
      961 tttcagaccg ctttggtcgt aagccagtcc ttattgccag ccagatcggc acattaatcg
     1021 gttttatcat gcttggtttc gccaacagta tggtggtcct tttcatctcc cgcatcattg
     1081 acggtctttc tggagcgaat atctctaccg ctcaagcagc gattgcagac agcaccaatg
     1141 aaaagactcg cacgcaaggg ttgggtttgg tgggtgccgc attcggtgta gggtttgtgt
     1201 tgggtcctat cttggctttc attgttttgg ctgcctcggg tcagaattat cagtacgtgg
     1261 catggacagc cgcgcttttc tccctcattt ctattttgct aacgacattc tggttcaaag
     1321 aaacagttgc agatgcagcc gaccccgaag cctcgcgcaa gcgccgtccg ttttcgtttg
     1381 gtaatttacg cgaagctatg gcacaccgcc ccaccatcat gttcctcgtg atcgtgatgt
     1441 tcttctatca aatcgctttt ggtggatatg aacaactctt ttcactcttc actctcaatc
     1501 gcctcggcat ggatgcgcgt gacacctctg gattgttcat gttggcaggc atccttatca
     1561 ttgtgatcca aggtggtttg atcggacgtt ggtctcgcaa aaagggtgac cgttggctgg
     1621 cattgatggg cattggtgcc ttagccatcg gcttgattgg cacggctctg actccagcca
     1681 tgcctgtgcc ttggtacgat aaggcgcgtg tactcgaaag ccttgccggg caaggtgagt
     1741 tccgtgtttc tattcaaagc atcaacgtca gtttgccaga tgaggcggct cgcggttggt
     1801 tcggtattat ttggttgatg atcgccagca tccctgccgc tttgggcggt gggacgctcc
     1861 agccagccat caatagctta atcacaaaag attctgataa gagcgaagtg ggtggaatgc
     1921 tcgggttatc ggctgcggca tatagcgcgg caaatgcgat tgcccccctg ttttatggtg
     1981 cattattcca gtggtttggt gcgccagttc cattctttgc gggcggagcg attttactgg
     2041 cgctgttctt atttgcccca agagtgatta agaactcata acgaaagaat gtgagcgcta
     2101 gacgaaggtc tagcgctcac ggtttatagt cagaatctcc aatagctttg ttgtatcttc
     2161 tacaatctca tcggcgccaa gatgtcttag ctctggctct tcgccaaaac cacatagcac
     2221 gccaagggtt tgtgctccag ccgatttgcc cgcacggata tcaaccgttg tatccccgat
     2281 catgaggcag ttttccggcg agacgttcat cttctgcgcc gcaagaagga tgggatcggg
     2341 atagggtttg gtgtgaaccg ctgaaagccc tgtaatcaca gcatcgaaat atttcacaag
     2401 gtcatactgc tcgagaaaac gcatggtccc tttttcatct cgggcgctga cgacagccat
     2461 gggatagcgt ccatgtaact gtttgagcat ctcatccaca ccggggacga gcaggaattt
     2521 tttatcagat tgtttgcggt gacgactcag ccaattgatc acagccacca tttcatcatc
     2581 caaatgaagc gtatcggcaa gaccaagcaa ggcattaccg ggagcttcga tccacatcac
     2641 gaaacgacgt gcggcatgtt ctgggtcctt aaaaagaaaa cggggaaaca atttcgacac
     2701 cttttgaacg tacaggtcat ctgtatcgct taaggtgcca tctacatcga agcatagtgc
     2761 atttatccta tatacgtcta gaggcatgga ttaattgtac caaaggggct atacttaagc
     2821 cgggataaga atgactgaag atcctaatcg tgccctcctc gaattattag taaaggtcag
     2881 ccgtgaggtt gccaccgcgc tggatctccg cacggtgttg caacgggttc tgtacgcttg
     2941 catccagtac gtgggcggtg agcgtggcag cattgtggtg atggatgacc ttggcaaacc
     3001 tgtagatgcc accattgtgt atggaaaaca attccatgat cacaccacac aacaacttcg
     3061 agaaacggtc gaacgtgggt tggcaggttg ggtggttcaa aatcggaaac cagcccttgt
     3121 ccctgatact agcaaggatg aacgttggct acgccgtgca gatgatgccg ccgaaaaaag
     3181 tggagccaag tctgcgatct gtgtgccgct tctggcgcgc gaacgctttg taggtgtgtt
     3241 gacgctggtt caccctgttc ccaatgcttt taatgaacaa catcttgaat tagtccaagc
     3301 gatctccgat caggcgagca ttgcagtgtt gaatgcccgc ttgtataccg agacccagcg
     3361 cacggcgcga gttatgacgg cgctggcaga aggcgcggcg acgatcaatt cttcgctgga
     3421 aatgcaggat gtattacgtc gcatcttgaa tcagactatg caagccttgc aagtggaaac
     3481 ggttgcgctg gggctgatcg actcgcatag caatgatctc gtatttcaag ccgcagctgg
     3541 aaataactct gggcatattc cggggttgca tgtaccagca ggaaaaggct tggtagggaa
     3601 agtgattcaa gccgggcgcg gagtgattct tcctagcatc aaacatgacc caacgttggt
     3661 cgaagaagac
//
LOCUS       DAOQFT010000225         3663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14267_length_3663_cov_8.891353, whole genome shotgun sequence.
ACCESSION   DAOQFT010000225 DAOQFT010000000
VERSION     DAOQFT010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14267_length_3663_cov_8.891353"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2274)
                     /locus_tag="PLR93_06800"
     CDS             complement(<1..2274)
                     /locus_tag="PLR93_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNH26709.1"
                     /translation="MTAFLPWYLILTLLGWLTFPLVYHLFPMLTDRGFTLARTAGLLI
                     WGYVFWLFVSFGFARNDIGGIVFALTILIALSVWSFFVHYTSILAFVRSKWTLIVTTE
                     VLFLIAFAFIAFVRAADSELASTEKPMELMFINSILRSETFPPQDAWLSGYAISYYYF
                     GYVLTAMLAQLSKINGSTAHNLMLSLVFALGAIGSFGIVYNLLSSFHSKKRSDEQGSI
                     SSVVSALLAPLFLLLVSNFEALLEVLHRAGIGWTKDAATQTWSGPFWTWLDMKELSQP
                     PVEPFGWVPDRYLWWWRASRVIQDYDIVGGFREAIDEFPFFSFLLGDLHPHVLAIPFG
                     LLAVSVALNIFLGGWKNGKVNFLGAQIHILPTGFLFAALVLGGLAFLNTWDILVAAAL
                     IVFAFVLWRANEEGWGWARLEDMLLLGLPLGILSLLLYFPFYIGFSSQAGGLLPNLMY
                     IARGTHLWVMWGTLLIPIYLFLITVLMKNPARPNWKLGFSIGLGTPLLFFLLAVILSL
                     LALIVEKDFTNALLASYNMTTGQFFAATSMRRLTHIGSLLTLVAVLIPAVAFVFSRNG
                     EQETSEKEDSASTSSSLQFLFLLLTLGTLLILAPEFVYLRDQFGYRINTIFKFYYQAW
                     ILWSLVAAFGVGYVLQSLRGVADITARILVGAVIFCGLLYPTFGIMTKTNNLKPVFGF
                     NLNDFARVQRENADDAAGIEFLLTQPEGVIAEAIGGSYSYYGRVATHTGYPTVLGWPG
                     HESQWRGGSELQGTRQDD"
     gene            complement(2294..2653)
                     /locus_tag="PLR93_06805"
     CDS             complement(2294..2653)
                     /locus_tag="PLR93_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HNH26710.1"
                     /translation="MILHITSKQDWSAAQANGEYIAPSLATEGFIHFSTDKQAVNVAN
                     AFYRGRTDLVLLVVDESRLKPELKWEAPAGPPAENISEADLFPHLYGPLNLDAVASVL
                     DFQPDSAAGTFSLPKLG"
     gene            complement(2725..>3663)
                     /locus_tag="PLR93_06810"
     CDS             complement(2725..>3663)
                     /locus_tag="PLR93_06810"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNH26711.1"
                     /translation="YVVAIEVVIAALIALFVFFLIEKAYIGFLALPLAFWSAVLILRS
                     DLPDTKRFILFVTGTALTITIAVEFLALVGDIGRMNTIFKLYLQAWMMFAVSAAAAFG
                     WLLPAFTQWRFKWRTIYQAGVYTLLGCAFMFTLTASTDKMSDRLNPDAPHSLDAMEYM
                     AHSQLWDGQIMELREDYNAIRWMQDNIEGSPVIVEGNCTEYRWCTRFTIYTGLPGVVG
                     WNWHQRQQRGIFAPQVQERVDQIGNFYTTTDVQVALAFLEKYDVHYIVVGQLERNIYP
                     TLPGTPDGLAKFAEYEGTYWDAVYTDANTTIYQVKP"
BASE COUNT         1110 a          877 c          928 g          748 t
ORIGIN      
        1 gtcgtcttgc cgtgtgcctt gcagttctga gccaccacgc cactgtgact cgtgaccggg
       61 ccagcctaag acggtggggt atcctgtgtg agttgccaca cgcccatagt agctataact
      121 tccgccaatc gcttcggcga tcaccccttc gggttgggta agcaaaaatt cgatgccggc
      181 ggcatcatca gcattctctc gttgtacgcg ggcaaaatcg ttgaggttga agccaaagac
      241 cggcttaagg ttattggtct tggtcataat gccgaaggtg ggataaagca acccacaaaa
      301 gatgaccgcc cccaccagaa tgcgagccgt aatatctgca actccgcgta ggctttgaag
      361 aacatagccc acgccaaagg ctgcgaccaa gctccagaga atccacgctt ggtagtagaa
      421 cttgaagatg gtattgatgc gatacccaaa ttggtcacgt aaataaacga actcgggggc
      481 aagaatgagc agagtaccca gagtaagtag tagaaaaaga aattgaaggg atgaagatgt
      541 agatgcagag tcttctttct cgcttgtttc ttgttcacca tttcgagaaa agacaaatgc
      601 tacagcaggg attaacacgg cgaccaaggt gagcaaactg ccaatatggg tcagtctacg
      661 catggaggtg gcggcgaaga actgcccggt ggtcatgttg taggaagcaa gcaaagcatt
      721 ggtgaaatct ttttcaacga tcaatgccaa taagctcaaa ataaccgcga gtaggaaaaa
      781 caacagaggc gtgcccaacc caatggagaa acccaacttc cagtttgggc gagccgggtt
      841 cttcatcaaa acagtaataa gaaaaagata aatcggaatc aacagcgtgc cccacatcac
      901 ccaaagatgg gtgccacggg caatatacat gaggttgggg agcaacccgc ccgcttggga
      961 agagaagcca atgtaaaacg gaaaatataa aaggagagac aagattccga gcggtaagcc
     1021 taacaaaagc atatcttcga ggcgtgccca gccccaacct tcttcattgg cacgccaaag
     1081 gacgaaggca aagacgatca acgctgcagc aacaagaata tcccaagtgt tgaggaaggc
     1141 gagcccgcca agtacaaggg cggcaaacag aaaacctgtt ggcaagatat ggatttgcgc
     1201 gcccaagaag ttgactttgc cattcttcca cccgccgaga aagatgttca acgccacaga
     1261 aacagccagc aagccaaatg gaattgccaa cacatgcgga tgaagatcgc caagcaggaa
     1321 agagaagaag ggaaattcgt cgatggcttc gcggaagccg ccaacgatgt cgtaatcttg
     1381 aatgacgcgt gaagcacgcc accaccatag ataacgatct ggtacccagc caaatggttc
     1441 aaccggcggc tgagacagtt ctttcatgtc cagccatgtc cagaaaggtc cgctccatgt
     1501 ttgggtggcg gcatcttttg tccagccgat gcctgcacgg tgcagcacct caagcaaggc
     1561 ttcaaaattt gaaacgagca aaaggaaaag cggagctaat aacgctgata caactgaaga
     1621 tattgagcct tgttcatcag atcgcttttt gctgtggaag gaacttagca ggttatatac
     1681 gataccaaag gaaccaattg cgccaagagc gaagaccagt gaaagcatca ggttatgtgc
     1741 tgtactgccg ttgatcttgg aaagctgagc cagcatggcg gtcaacacat agccgaagta
     1801 ataatacgaa atggcatacc ctgaaagcca ggcatcttgc ggtgggaagg tttctgaacg
     1861 caaaatggaa ttgatgaaca tcaattccat tggtttttca gtgctggcaa gttccgagtc
     1921 tgcggcgcgg acgaaggcaa taaaggcaaa ggcgatcaga aacagaactt ctgtggtgac
     1981 gatcaacgtc cacttggagc gcacaaaagc aagaatggac gtgtaatgaa caaaaaagga
     2041 ccaaacgctt aaagcgatca agatcgtcaa cgcaaaaacg atcccaccaa tatcattgcg
     2101 cgcaaaacca aaagaaacaa atagccaaaa aacatatccc cagatcagca aaccggctgt
     2161 ccgcgcaagg gtgaagccgc ggtcggtaag catggggaaa agatgataga ccagcggaaa
     2221 ggtcagccag ccaagcaatg taaggatgag ataccaaggg agaaaagcgg tcatctagtc
     2281 agtactcagt gggttaaccc aacttcggga gagaaaacgt tccggcagca gaatcaggct
     2341 gaaagtccag aacagaggcg acagcatcca aattcaacgg accatacaga tgcggaaata
     2401 aatctgcctc agaaatattt tcagcaggcg gacccgctgg cgcttcccac ttaagctcgg
     2461 gcttgagtct gctttcatca accaccagca agaccaaatc ggttcgcccg cgataaaaag
     2521 cattcgcaac gttgacagcc tgtttgtctg tagaaaagtg aatgaagcct tcggttgcaa
     2581 ggctgggagc gatatactcc ccgttcgctt gtgcagcaga ccaatcttgt ttggatgtga
     2641 tgtgaagaat catgggagta cctcagtgat tatgattcgt gagacactct ttgcatactc
     2701 tataaagagt gtctcacgat gtgattaagg cttgacttga taaatggtcg tattcgcgtc
     2761 ggtatagacg gcatcccaat acgtgccttc gtattcggca aattttgcca gaccatcggg
     2821 cgtgccgggc aaggttgggt aaatattacg ctcaagctgc ccaaccacaa tgtagtggac
     2881 atcatacttt tcaaggaatg caagcgcaac ctgaacatcg gtagtggtgt agaaattccc
     2941 aatctggtca acccgttctt gtacttgggg cgcaaatata ccgcgctgct gacgctgatg
     3001 ccagttccag cccaccacgc cgggcaaacc agtgtaaatg gtaaagcgcg tacaccagcg
     3061 atattctgtg cagttgcctt ccacgattac tggtgaccct tcaatattat cttgcatcca
     3121 gcggattgcg ttgtaatctt cgcgcagttc cataatctgc ccatcccata attgagaatg
     3181 agccatgtac tccatggcat ccaatgaatg cggagcatct gggttcaacc ggtcactcat
     3241 tttgtcagta gatgcggtaa gggtgaacat aaacgcacaa cccaagaggg tatacacacc
     3301 tgcttgatag atcgtacgcc acttaaagcg ccattgagta aaggcaggta ataaccagcc
     3361 aaacgctgct gcggcactaa cggcaaacat catccatgct tgcaagtaca acttgaagat
     3421 ggtgttcata cgtccgatgt cgccgaccaa cgccaggaac tctacggcga tggtgatggt
     3481 caacgcggta cctgttacaa aaaggataaa gcgcttggta tcgggcaggt ctgaacgtaa
     3541 gatgagcacc gcactccaaa atgcgagcgg aagtgctaag aatccaatat atgccttttc
     3601 aataaggaag aagacgaaga gcgcgatgag agccgcaatc accacttcga tggcgaccac
     3661 ata
//
LOCUS       DAOQFT010000226         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14289_length_3659_cov_6.820477, whole genome shotgun sequence.
ACCESSION   DAOQFT010000226 DAOQFT010000000
VERSION     DAOQFT010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14289_length_3659_cov_6.820477"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLR93_06815"
     CDS             complement(<1..964)
                     /locus_tag="PLR93_06815"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNH26712.1"
                     /translation="MQWQTNPYFAPIIISSLIAIVCAFFVITRRNRASGSTALLGLIF
                     SVIIWGFAYAMELASVSLKWQIFWAKVEYLGIPFVPLFFFIFAYQFTQSTSKLSRRVI
                     FFLVVISSIFVLLAWTNEFHNLIWTELGQKNIDGYYMLTLGHGMAFWLLITYSYILLA
                     IASISIIRRALSGPAEIRKQTYIILVGASVTWLGNIIYISGLSPVPELDLTPIGFVIS
                     SVIFSFGIFGYGMLDIMPIAGEFILESLDDVVIVTNPKGMLIYVNRAFEYYFSNPVQS
                     LIGRQATEAFSNLPVLKSLFDSSNTIRREITVTPENRPLFIFNVR"
     gene            complement(1223..1627)
                     /locus_tag="PLR93_06820"
     CDS             complement(1223..1627)
                     /locus_tag="PLR93_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26713.1"
                     /translation="MKKFNIKSLLVFVVALIMVLSLSACATEEVSDASATDEAATEVA
                     TEVATEVATEEAGVIVPEFTFNNTGEEEICEIYLSPVGQDQWGPDQLGGTTIPSGGSY
                     VLQNIPAGQYDVKAVGCAGSEVIGQLDIQPQQ"
     gene            1836..3098
                     /locus_tag="PLR93_06825"
     CDS             1836..3098
                     /locus_tag="PLR93_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH26714.1"
                     /translation="MPTTDMLKRDVIIIGGGPVGLSCAYYLLKSGRQVTLLDAKEIGK
                     GSGSGNAGHIVPSHIIPLAAPGVVTSALKWMLDPAHSPFGMKVSLNPNYLMWLLRFTI
                     ACSQNNVDRSLEPLNQLGQLSAKNFAQIIAEEKFDCAYQEMGLLHLFKTEKAFHEGQH
                     EGEFMQRHGIPVSVYDKNKIHEVEPATVDDVIGGVHFTGDAHLNPAVFLNLLKERVKS
                     MGAELMDGTQVTGFETANGKITKVKTSAGDFEAEQIVLAAGALTPSVAKDLKLNIPVQ
                     PARGYSMTMSATKTMPSHALILGERRIAVSPMAGLLRFTGRLEVGNYSMEPNPIWIQR
                     IETSAREYLRLDEKLDVKETWAGLRPTTPDGVPIIGKSPKHDNLVLATGHAMLGLSLG
                     PGTGAVVSQLLNGQETAFSLSPMRLERF"
     gene            3177..>3659
                     /locus_tag="PLR93_06830"
     CDS             3177..>3659
                     /locus_tag="PLR93_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26715.1"
                     /translation="MTKVKVLWFLSVALLSACSNAEPSPTSTPEPTTTPTSTPIPMPS
                     QVSEINLPDDPMNAPYGIALNSLGNLYVNDAGNNRVLIFDADGNLLSKWDTQGSGDGE
                     FKTLGFGSLAIDADDNVFIVDNGNSRIQKFDKDGNFILQWGAQGKEDGQFIRAIGIAI
                     D"
BASE COUNT         1019 a          777 c          930 g          933 t
ORIGIN      
        1 tccgcacatt gaagatgaac agcggacgat tttcaggcgt gaccgtaatt tctctgcgaa
       61 tggtatttga gctatcaaat agagatttca gaacgggtaa gttggaaaaa gcttcggtag
      121 cttgcctgcc gatcagggat tgtacggggt tgctgaagta atattcaaag gcacggttga
      181 catagatcaa cattcccttg gggttggtga cgataactac atcatccaag ctttcgagaa
      241 tgaactctcc ggcgatgggc ataatgtcta gcatgccgta cccaaagatg ccgaagctga
      301 agatgacact ggagatgacg aagccgatcg gggttaagtc taattcgggc accgggctca
      361 aaccggaaat gtaaataata ttccccaacc atgtgacgct tgcgccgacc aaaatgatat
      421 aggtttgctt cctaatttca gcgggtccgc ttaaggctct acgaatgatg ctgatcgaag
      481 caatagccaa tagaatatac gagtaggtga ttagaagcca gaacgccatg ccatgcccaa
      541 gcgtcagcat ataatagcca tcgatatttt tttgaccgag ttcggtccag atcaaattgt
      601 ggaattcgtt agtccatgcc agaaggacga atatggagga aataacgacc agaaagaaaa
      661 taacgcggcg cgataatttg ctggtgcttt gcgtgaattg ataagcaaat atgaaaaaga
      721 ataaaggcac aaaaggaatg ccaaggtatt ccacctttgc ccagaatatc tgccatttca
      781 ggctgacact ggcaagctcc atggcgtagg caaatcccca aatgatgacg gaaaagatca
      841 acccgaggag cgctgtcgag ccagatgccc ggtttctgcg cgtgatgaca aaaaatgcac
      901 agacgatggc gattaagctg gaaatgataa ttggagcaaa atatgggtta gtttgccatt
      961 gcattggaac gttttcagac ctttgaaaag ttaattttag aaacaccctt tggctgaaat
     1021 gtttcaaaga aattcagtga attattcatg ggttcgtggg tttgtcaaga cagggttatg
     1081 caattgcaag aaacctttac aacccatccc atgttttaga gcgccgctca ttataatggc
     1141 atatagcgag tgaatttaga gcggatatgt cataaataaa aagacctctg agaaaaactc
     1201 agaggtcttt tctgtcattc aattattgtt gcggttgaat gtcgagctga ccgatcactt
     1261 cggagccagc gcagccaacg gctttgacat catactgacc agcgggaatg ttctggagaa
     1321 cataggaacc accggagggg atggtggtgc caccgagctg atcgggaccc cattgatcct
     1381 gaccgacagg ggagaggtag atctcgcaga tttcttcttc gccagtgtta ttaaaggtga
     1441 attcaggaac gatcacacct gcttcttcag tagcgacctc ggtagccact tcagtggcaa
     1501 cttcggtggc agcttcatca gtagcggaag catcgctgac ttcttcagtt gcgcatgcag
     1561 aaaggctgag aaccatgatg agggcgacga cgaacacgag caaagacttg atgttgaact
     1621 tcttcattgt attcctttat gttgtttaga ttacgtgcgg gcgcggatta tacataaaaa
     1681 agaaaaaagt gcaacctatt tttgattata aaattattaa tttggaatct ttttaatggt
     1741 tctcatattt ggattcggtc aacatagact caagcggctc tggaatataa tgacttgaaa
     1801 tattcaaact tgaatgatct ttggagaaga taacgatgcc tacaacagat atgttgaaac
     1861 gcgatgtgat tatcattggt ggggggcccg tggggttaag ttgtgcgtat taccttttga
     1921 aatcgggcag gcaagtgact ctgctggatg caaaagagat tggcaaaggc agcggttcag
     1981 gcaatgccgg gcatatcgta ccgagtcaca tcattccact cgctgcacct ggtgtggtga
     2041 cctctgccct taaatggatg cttgacccgg cacatagtcc ctttgggatg aaagttagcc
     2101 tcaacccaaa ttacttgatg tggctattgc gttttacgat agcgtgtagc cagaacaatg
     2161 tggatcgctc attggagccg ttgaatcaac tcggtcaact tagcgcgaag aattttgctc
     2221 agatcattgc cgaagaaaaa tttgattgtg cctatcaaga gatgggacta ctgcatttat
     2281 tcaaaaccga gaaagccttc catgagggtc agcacgaagg cgaattcatg cagagacatg
     2341 gcattcctgt cagtgtatat gacaaaaaca aaattcatga agtcgagccc gccaccgtgg
     2401 atgatgtcat tgggggcgtg catttcactg gagatgcgca cctcaacccg gcggtatttc
     2461 tcaatttgct gaaggaacgt gtcaaatcta tgggcgcgga gttgatggac ggcacgcagg
     2521 tcacgggctt tgaaactgca aatggcaaaa ttacgaaagt caaaacgtct gctggagatt
     2581 ttgaagcgga acagatcgtg cttgccgctg gcgcgttgac tccctctgtt gccaaagatt
     2641 tgaagttgaa cattccggtt cagcctgcac gtggatacag catgaccatg tccgccacga
     2701 agaccatgcc gagccatgcg ttgattctgg gcgaacgccg catcgccgtc tcgccgatgg
     2761 ctggacttct gcgcttcaca ggtcgactcg aagtgggcaa ttacagtatg gaaccgaatc
     2821 cgatttggat tcaacgcatc gagacttcgg cgcgagagta tctgcgtttg gatgaaaaac
     2881 tggacgtcaa agaaacatgg gcgggcttgc gtcccaccac gcctgatggc gtgccgatca
     2941 ttggcaagtc accgaagcat gacaatcttg tgctggcaac cggtcacgcc atgctcgggc
     3001 tctcgttggg acccggaacc ggcgcggtcg taagtcaact gctcaatggg caggaaacag
     3061 ccttcagttt aagtccgatg cggttggaac ggttttagtc aaaaccattc tagaggatgg
     3121 tgtcatcttg cttttgtgta cctataatcc tataaatctt atgaggagta gagtacatga
     3181 ccaaggtgaa agtcttgtgg ttcttaagtg ttgctttatt gtccgcttgt agtaatgcag
     3241 agccttctcc aacgagtacg cctgaaccaa caaccacccc tacgagtaca cccataccca
     3301 tgccttctca agtttccgaa ataaacctgc ctgatgaccc aatgaatgca ccgtatggaa
     3361 ttgccttaaa ttcgcttggc aatttgtatg tcaacgatgc tggtaataac cgtgtgctga
     3421 tttttgatgc agatggaaac ctcttatcca aatgggacac acaaggtagc ggcgacggcg
     3481 aatttaagac cttgggcttt ggtagtttgg caattgatgc ggatgataat gtcttcatag
     3541 tggataatgg caatagccgc attcagaaat ttgataaaga tggtaacttc atcttgcaat
     3601 ggggtgctca gggtaaagaa gatgggcagt tcattcgtgc aattgggatt gcaattgat
//
LOCUS       DAOQFT010000227         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14309_length_3657_cov_6.761799, whole genome shotgun sequence.
ACCESSION   DAOQFT010000227 DAOQFT010000000
VERSION     DAOQFT010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14309_length_3657_cov_6.761799"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /gene="priA"
                     /locus_tag="PLR93_06835"
     CDS             <1..1898
                     /gene="priA"
                     /locus_tag="PLR93_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNH26716.1"
                     /translation="RPKNIRVAQLAVTPEAAEEMMASLGSTKATQTRRESALRFLIQQ
                     PEPIHLSWVYAQTGCNISDLQELEERGLIRLFENEIFRDPLERIEKQETKSELSLTSE
                     QNSALQAILSSFRSQPSGFLLYGVTGSGKTEIYLRAAQEVIKSGRQAIILVPEIALTP
                     QAVRRFVSRFPGQVGLVHSKLSEGERYDTWRRARDGKLNVIIGARSALFAPLPNIGLI
                     VVDECHDSSYYQSEPPYYNAVTAAQEYAKLCGAVCVLGSATPTIEQRFLAEQNQITRL
                     DLPNRVTESTLPPVQVVDMREELKNGNRGIFSKPLAESLAEVLERKEQAIVYLNRRGT
                     STYIFCRDCGTTLKCPSCETPLTLHNEIHYAKGNTLLCHRCGYTRQMPKTCPTCASTQ
                     IREYGLGSERVEAEIKSMFPQARTLRWDWDTTRQKDAHEIILTHFANHQADILVGTQM
                     LAKGLDLPLVTLVGIVLADVGLNLPDPFASERVFQTLTQVAGRAGRSSRGGKVIMQTF
                     TPDHYALQFAAGHNVDGFYERELNYRRSLGYPPFTKLVRLEYRNTDNLKAEEEAKKLA
                     SKLKVKLEAGGFRQTELIGPVPCFFAKENGEYRWQIVLRGPNPEAVLRDFRINDWRIE
                     VDPINIL"
     gene            complement(1932..>3657)
                     /locus_tag="PLR93_06840"
     CDS             complement(1932..>3657)
                     /locus_tag="PLR93_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HNH26717.1"
                     /translation="DLFEKAFGSSDAVTFENVTYAIAAFERSLVTNNSPFDRYAAGDV
                     NALTPSQRRGLGLFRSAATRCFECHAAPTFADESFSVTGVPDLPGQPHDEGRKAIEPS
                     GLDGAFKAPTLRNIALTAPYMHNGVFNTLEEVVDFYAKGGGRDAGVQDVDIHVLGFEL
                     TEQEKADLVNFLYALTDESNLPEIPTSVPSGLDVVKKLEPVQARQVVSEINAAESAAG
                     STSTHEPVTLRVEPGQTIQEVVDQALPGDTIEVPYGIYEEHVILDVSDLKFFGIPNEK
                     GEWPIIEGNGTGSDGVIASGNNFEMAFFQVKNFTSNGVLIEGSTGVYLHDMYIENTGV
                     YGVYPVRCTDVLIERIEGTLMNDAAIYAGKSKDVVIRDTLTYGNVIGIELENTVNGEV
                     YNNYAHDNTIGIFIDLLPQLPSKVSMNTKVYNNTSENNNGANFGKPGSAVSLIPPGTG
                     MLILAADHVEVYGNTFTGNKTVGLAVFNLTIGFSPEEIDVDPNPDHNFAHDNIYENNG
                     YDADPFVKNMLGSGFDIVWDTTGVDNRFDEPNAKTSFPPALPKSTWPQPLYNLYWRVL
                     HFAVGLIS"
BASE COUNT          953 a          921 c          897 g          886 t
ORIGIN      
        1 tgagacccaa gaatattcgc gttgcccaac tcgcagtcac gccagaggcg gcagaagaaa
       61 tgatggcatc gcttggttcc acgaaagcga ctcagactcg acgcgaatcc gctctacgct
      121 tcctcatcca acagcccgaa ccgattcatc tttcatgggt gtatgcgcaa acaggctgca
      181 atatttcaga cttgcaagag cttgaagaac gcggtctgat tcgcctgttt gaaaatgaaa
      241 tctttcgcga cccgttggag agaattgaga aacaagaaac aaagagtgag ttatcgctta
      301 catcagaaca aaattctgct ttgcaagcca ttctttcatc cttccgctct caaccttctg
      361 ggtttctttt atacggcgtg accggttctg gcaaaacgga aatttactta cgcgccgcac
      421 aagaagtgat caaaagcgga agacaagcca tcattcttgt gcctgaaatt gcgctcaccc
      481 cgcaagctgt gcggcgcttt gtctcacgtt ttccgggtca agtcggtttg gttcactcga
      541 agctttctga aggggaacga tacgatacat ggcgtcgtgc ccgcgatggg aagttgaacg
      601 tcattattgg ggctcgttcg gcgttgtttg cgcctttgcc aaatattggt ctcatcgtcg
      661 tggatgaatg ccatgacagt tcatactatc aaagcgaacc gccttattac aacgccgtga
      721 ccgccgcaca ggaatatgca aaactttgtg gcgcggtttg tgtgctgggc tctgccacgc
      781 ccacgattga gcagcgtttc cttgcggagc aaaaccagat cacacggctc gacttgccca
      841 accgtgttac cgagtctaca ttgccgccag tgcaagtagt agacatgcgt gaggagttga
      901 agaacggtaa ccgcggcatt ttctcgaaac cattagccga gtcactagcc gaagtcttgg
      961 agcgcaaaga acaagccatc gtctatctca accggcgtgg aacatcgaca tatattttct
     1021 gccgcgattg tggtacgaca ttgaaatgtc ccagctgcga aacaccgctg acattgcata
     1081 atgagattca ttatgccaaa ggcaatactt tgctctgtca ccgttgcgga tacacccgtc
     1141 aaatgcccaa gacttgcccc acttgtgcga gcacgcaaat ccgcgagtat gggctgggaa
     1201 gtgaacgggt agaagcagag atcaaatcca tgttcccgca agcgcggacc ttgcgctggg
     1261 attgggatac aactcggcag aaagatgctc acgagatcat actcacccac tttgccaatc
     1321 accaagccga tattttagta ggcacgcaaa tgctcgccaa agggttagac ctgcccctcg
     1381 taaccttggt tggaattgta ttggcagatg ttgggttgaa tttgcccgac cccttcgcaa
     1441 gtgaacgcgt ctttcaaacg ttgacgcagg tggcagggcg ggcggggcgt tcttcacgcg
     1501 gcggcaaggt cattatgcag acctttacac cagaccatta tgctcttcaa tttgcagccg
     1561 ggcataatgt agatgggttt tatgaacgcg agttgaacta tcgccgttcg ttagggtatc
     1621 ccccatttac aaagttagtg cgtttggaat atcgtaatac ggataatctc aaagcggaag
     1681 aagaagccaa gaagctggca agcaaactca aggtcaaatt agaggcgggc gggtttcgtc
     1741 aaacagaatt aattggaccc gtcccttgtt tctttgcgaa agaaaatggc gagtaccgtt
     1801 ggcagatcgt cttacgcgga ccaaacccag aagctgtgct aagagatttt cgaatcaacg
     1861 attggcgaat agaagtagac cccatcaata ttttataaaa agagggcgac ctttcggtcg
     1921 ccctcttttt attacgaaat caagcctaca gcaaagtgta agacacgcca atacaagtta
     1981 tacaagggct ggggccatgt gctcttgggc aacgcaggcg ggaaggacgt tttcgcattc
     2041 ggttcgtcga agcgattatc cacgccggtg gtatcccaca caatatcaaa cccactaccg
     2101 agcatattct ttacaaacgg atcggcatca taaccattat tctcgtagat gttatcgtgt
     2161 gcaaagttat ggtcgggatt ggggtctaca tcgatctctt cgggtgagaa accaatggtg
     2221 agattgaaga ccgccaagcc tacggtttta ttgcccgtga aggtattgcc atacacttcc
     2281 acatggtctg cggcgaggat gagcatgcct gtgccaggtg ggatcaatga aacagcagag
     2341 ccgggcttac caaagtttgc gccgttgttg ttctcagagg tgttgttata caccttggta
     2401 ttcatcgaaa ctttggaagg caattgcggc aataagtcga taaaaatacc gatggtgttg
     2461 tcgtgagcgt aattgttata cacttcgccg ttgactgtgt tttccaattc gataccgatc
     2521 acgttaccgt atgtcaatgt atcgcggatg accacatcct ttgacttacc tgcatagatg
     2581 gcagcgtcgt tcatcaaagt cccttcgatg cgttcaatca acacatccgt acagcgcaca
     2641 gggtaaacac catacacacc cgtgttctca atgtacatat catgcaagta aacgccggtt
     2701 gaaccttcta tcaacacacc attgcttgta aagttcttta cttggaagaa tgccatttca
     2761 aagttattgc cagaagcaat cacgccatca gaacctgtgc cattgccttc aatgatgggc
     2821 cattcaccct tctcattcgg aataccgaag aacttgagat cgctcacatc gaggataacg
     2881 tgttcttcat aaattccata agggacttcg atggtgtcac cgggcaatgc ctgatcgaca
     2941 acttcctgaa tggtctgacc cggctcgact ctcaaggtca ccggctcatg cgtagacgtg
     3001 gagcctgccg cactttcagc agcattgatc tcgctgacaa cttgacgcgc ctgcacaggc
     3061 tcgagctttt tcaccacatc caagcctgaa ggcacagagg ttgggatttc cggcaaattg
     3121 ctttcgtctg tcaacgcata aaggaagttg accaggtcgg ctttctcctg ctcggtcaac
     3181 tcaaagccga ggacgtgaat atccacatcc tgtacaccag catcacgtcc gccgcctttg
     3241 gcgtaaaaat ccaccacttc ttcaagcgtg ttgaatacac cattgtgcat gtagggggca
     3301 gtgagagcaa tgttacgaag cgtaggagct ttgaaggcac catccaaacc agacggttca
     3361 atggcttttc gtccctcgtc atgcggctga cctggcaaat cgggcacgcc tgtgacagag
     3421 aatgactcat cggcaaaggt tggcgcggca tgacattcaa aacagcgcgt cgccgccgag
     3481 cggaagagtc caagtccgcg acgctgcgat ggagtaaggg cattcacatc gccagcggca
     3541 tatcgatcaa atggcgagtt gttcgtcacc aatgaacgct caaaagcggc aatggcatag
     3601 gtgacatttt caaaggtcac agcatcagaa gatccaaatg ccttttcgaa aagatca
//
LOCUS       DAOQFT010000228         3646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14383_length_3646_cov_4.683375, whole genome shotgun sequence.
ACCESSION   DAOQFT010000228 DAOQFT010000000
VERSION     DAOQFT010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14383_length_3646_cov_4.683375"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..339)
                     /locus_tag="PLR93_06845"
     CDS             complement(121..339)
                     /locus_tag="PLR93_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNH26718.1"
                     /translation="MTTVTYTVPAISCGHCTHTIESEVSELQGVQSVKAEIDTKKVTV
                     IFDTPADEAKIKALLAEINYPAEGLLTL"
     gene            complement(368..781)
                     /locus_tag="PLR93_06850"
     CDS             complement(368..781)
                     /locus_tag="PLR93_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNH26719.1"
                     /translation="MLLKKVLAVLLFAVFLSACAGGSASTKVTVTMTEFGFEPNTISV
                     TAGSPVELTLVNEGAIEHDFVIVVIPVTNISSSNSGDHHMATDEHSEYDLHTSTAAGE
                     TSTLTFTPTESGTYQIICSVPGHKDAGMTGELIVK"
     gene            complement(856..1125)
                     /locus_tag="PLR93_06855"
     CDS             complement(856..1125)
                     /locus_tag="PLR93_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNH26720.1"
                     /translation="MENEATLRRLKTVEGHLRGVIRMVEEDAYCIDVIRQIQAIEAAL
                     NKVSSKILEDHLNSCVITAIQGNDKKERERVLKEITEVFEMSTKV"
     gene            1266..1967
                     /locus_tag="PLR93_06860"
     CDS             1266..1967
                     /locus_tag="PLR93_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH26721.1"
                     /translation="MNISSKDLKQVVVFQHATDEDLNLILKNSITRSMEEGGFFFLQG
                     DEATYLYILTSGQVKLMQSNPNGQQVNLRTIYPWQMFGALGAVRATGATYPASAQTLE
                     DCSALAIPSLFLHSMLETRPYLSFDLMNLMTSYIQEMQARYRELATEKVEQRVANALI
                     RLAGQSGTRSENEAGIELSFSRQDVAEMTGCTLYTISRLLSDWERKGIIETGREKIRI
                     IQPHELVRIADGQEK"
     gene            2055..2285
                     /locus_tag="PLR93_06865"
     CDS             2055..2285
                     /locus_tag="PLR93_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26722.1"
                     /translation="MSDEFNISKTTTVEDLIKSNSKAVRFFLDRHLACAGCGFARFCT
                     LETVSKTYKLDESHFIEDVQKLVASSNLIWSA"
     gene            2285..>3646
                     /locus_tag="PLR93_06870"
     CDS             2285..>3646
                     /locus_tag="PLR93_06870"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNH26723.1"
                     /translation="MKTYLKFSTFVILLGILISACASVSSQPVGKEYVLTTDLRNGNL
                     VFLGVSDEINGQENPTLNAKPGERITVTLINGGEGSHSITFPELKASSEIVKEKGEET
                     SITITVPNVHGEIGYYDGVGNHADLGMHGKLVVTETGASMPASLTTSNGDPAVLAAFQ
                     KGACGSCHTISGIPGAVGVIAPKLDDISMMAEMHIKDSTYTGSAATAEEFIHESIIDP
                     NLFIAPDCPTGACPPNVMPATLSQTLTTDEVNSIVTYLSSLPEGAYTESTGTAETSAA
                     VAEPDNSGVDIVRDPADIPAPIGDREPTTVRIDLEAIEVTGQLADGTTYSYWTFNGVV
                     PGPFLRVRVGDTIEVHMKNSTSSSMNHSVDFHAVTGPGGGAVMTQTEPGKETMFTAKA
                     LNPGLFVYHCATPMVAQHITNGMYGLILVEPEGGLPPVDREFYIMQGELYTAGSFGEQ
                     GHQM"
BASE COUNT          938 a          944 c          860 g          904 t
ORIGIN      
        1 agttggcaca ggtcatgccg gtgatgggga gtgtgagttg ctttgtttcg gtcatgggtc
       61 ttccttacga attacggatt acgaaatgcg taatccgtaa ttcgtgatgc gtaaaaatct
      121 ttacaaagtg agcaaacctt cagcggggta gttgatctct gccagcaacg ccttgatctt
      181 cgcttcatca gcgggggtat cgaagatgac ggtaactttc ttggtgtcga tctcagcctt
      241 tacagactgg acaccctgca actcactgac ttcgctttcg atcgtgtgag tgcaatgtcc
      301 gcaggaaata gcgggaacgg tgtaagtaac ggtagtcatg atattttttc tccttgaatt
      361 tagtttttta tttgacaatt aactcgcccg tcatcccggc atctttgtgc ccaggcaccg
      421 agcaaataat ttgatacgtt ccggattctg tgggtgtgaa tgtgagcgta ctggtctcac
      481 ccgccgcagt ggatgtgtgc aggtcatact ctgaatgttc atcggtagcc atgtgatggt
      541 cacccgagtt ggaactggaa atattcgtca ccggaatcac tacgatcaca aagtcatgct
      601 cgatcgcgcc ttcgttgacg agagtcaact caacgggtga accggctgtc acgctgatgg
      661 tattgggctc aaaaccaaat tctgtcatcg ttacggtgac tttggtggac gccgatccgc
      721 ctgcacaagc agaaaggaac acggcaaaca acagaacggc taaaactttc ttcaacaaca
      781 tgcgaaactg cctccaaaaa atggtatttc aaatgctctt tcaggggcta agcccctgaa
      841 agagctagag actatttaaa ccttcgtaga catttcaaac acttcggtaa tttccttcaa
      901 cacccgttca cgctcttttt tgtcattgcc ttgaatggca gtgatcacgc acgaattcaa
      961 gtgatcttcc aatatctttg agctgacctt attcaacgcc gcctcaatcg cctgtatctg
     1021 acggatgacg tcgatgcaat aggcatcctc ttccaccatg cggatcaccc cacgcaggtg
     1081 tccttccact gtctttaatc gtcttagggt cgcttcgttt tccataggta cctctagttt
     1141 ccagatacag tccgttgagt tcctcttacc ccccccaggg gggtagggat tggcaaaata
     1201 tacgccatcc cctgcccctt cgtcaagtcg acaaatgtca tcggattggg gtgtaaaatg
     1261 actttatgaa tatttcttcc aaagatctca aacaggttgt cgtctttcag catgcaacgg
     1321 acgaagactt gaatcttatt ctgaaaaata gcatcacccg ttccatggaa gaaggtggct
     1381 ttttcttttt gcaaggcgac gaagccacgt atctgtacat cctcaccagc ggacaagtca
     1441 aactgatgca gtcaaatccc aatgggcaac aagtcaacct gcgtaccatt tatccgtggc
     1501 agatgttcgg tgcgttggga gccgtccgcg caactggagc cacctatcct gcttcagctc
     1561 aaacactgga ggactgttcg gctcttgcga tcccgagcct gttcctacac tccatgcttg
     1621 agactcgtcc atatctttcc ttcgacttaa tgaatctgat gacctcatat attcaagaga
     1681 tgcaggcacg ctaccgcgaa cttgccacgg agaaggtgga acaaagagtg gccaacgcat
     1741 tgatccgctt ggctgggcaa tccggtacga ggtccgaaaa cgaggcgggg atcgagttat
     1801 ctttctcccg acaggatgtg gcggagatga ccggatgcac actctacacc atcagccgcc
     1861 tgctcagtga ttgggaacgc aaaggcatca tcgaaacagg ccgggaaaag atccgcatta
     1921 tccagccgca tgaattggtt cgcattgcag acggtcaaga aaaataaatg acaatttgca
     1981 acaaataact ggcaggttgt aatttgcgca agcgcaaaga agagtaaagg gggatgcgat
     2041 attctgtgtt catcatgagc gacgaattta acatctccaa aacaacaact gttgaagacc
     2101 tcatcaaaag caattcaaag gcagtgcgct tctttttaga tcggcatctg gcttgtgccg
     2161 ggtgcggctt tgcccgcttt tgcacgttgg aaactgtcag caagacctat aaattggatg
     2221 agtctcactt tattgaagat gttcaaaaac ttgtcgcctc ctcaaactta atttggagtg
     2281 cataatgaaa acatatctta aattttccac ctttgttatt ctgctcggta ttttgatctc
     2341 ggcatgtgcg agtgtaagct ctcagcccgt aggtaaagaa tatgttttaa caacagacct
     2401 gcgcaatgga aacctggtct ttctgggtgt gagcgatgag atcaacggac aggagaatcc
     2461 cacactcaac gccaaaccag gcgaacgcat caccgtcaca cttatcaacg gcggagaagg
     2521 ctcccacagc attacattcc cggaattaaa ggcaagctct gagatcgtga aggaaaaagg
     2581 cgaggagaca tcgattacca tcacagtgcc caatgtgcat ggcgagatcg gctattatga
     2641 cggagtaggc aaccatgccg acctcggcat gcatggcaaa ttggttgtga ccgaaaccgg
     2701 tgcatccatg cctgcctccc taacaaccag caatggcgac ccggctgtac tggcagcttt
     2761 tcaaaaggga gcctgcggtt cctgccatac catctctggc attccgggtg cagtcggtgt
     2821 gattgcccca aaattggatg atatttccat gatggctgaa atgcatatca aggattcaac
     2881 ctacacagga agcgcagcca cagccgaaga attcatccat gaatcgatca ttgaccccaa
     2941 tctgttcatt gcccccgact gccccactgg cgcatgtcct cccaacgtca tgccagccac
     3001 tctttcacaa accctaacga cagatgaggt caattccatt gtcacgtatc taagcagcct
     3061 tcccgaaggt gcctataccg agtccacggg cactgcggaa acttcagccg cggtcgccga
     3121 acccgataat agcggtgtag atattgtccg tgatcccgcc gatatcccgg cacccattgg
     3181 tgatcgcgaa ccaaccactg tccgcattga ccttgaagcc attgaagtaa ccggtcagct
     3241 tgctgatggc accacctact cctactggac gttcaatggc gtagtgcccg gcccatttct
     3301 ccgtgtacgc gttggcgaca ccattgaagt acacatgaag aacagtacct ccagcagcat
     3361 gaatcattct gtggattttc atgcggtcac agggcctggc ggtggtgcag taatgaccca
     3421 aaccgaaccc ggcaaagaaa ccatgttcac cgccaaggca ctcaaccccg gattgttcgt
     3481 ctatcattgc gcaacgccaa tggttgccca acatattacc aacggcatgt acgggttgat
     3541 ccttgtcgaa ccggaaggcg ggcttcctcc tgttgatcgt gaattctata taatgcaagg
     3601 agaactttac acggcaggct cattcggcga acaggggcac caaatg
//
LOCUS       DAOQFT010000229         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14393_length_3644_cov_6.075508, whole genome shotgun sequence.
ACCESSION   DAOQFT010000229 DAOQFT010000000
VERSION     DAOQFT010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14393_length_3644_cov_6.075508"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="PLR93_06875"
     CDS             <1..143
                     /locus_tag="PLR93_06875"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNH26724.1"
                     /translation="HLSNVYAREEFRHTSFVSAVCKGKVTGFGWRSYELGLRGLVDIM
                     RA"
     gene            140..928
                     /locus_tag="PLR93_06880"
     CDS             140..928
                     /locus_tag="PLR93_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH26725.1"
                     /translation="MNTQIIPTTEPFFLPGGRTGVLLIHGFTGGPKEMRWMGDYLNQQ
                     GYTCLGIRLAGHATTPEDMIRSRWTDWTTSVEDGYNLLRGATDKIFLVGLSMGGVLSL
                     LMSTRLDVAGVLAMSTPYQLPRDEKDYSPEFIEFYSRFVKFMPKSTEVPGASWFDKEA
                     FQSHISYPQNPIRSIAEMKRLILVMRAALPNVTKPVLLIHSKNDKYVLPENVERLYDG
                     LVNASDKTKAYVVEAGHVVTRDAARHQVFEMARDFIRRVDASHS"
     gene            949..2076
                     /locus_tag="PLR93_06885"
     CDS             949..2076
                     /locus_tag="PLR93_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26726.1"
                     /translation="MNRNLSFIAAALLLWGFGEGMFFNFVPIYLDVQFLLSKYEIGLI
                     LGGFGFFMAITHIPAGRLADRLGRKPLLIVAWLLGLLSTLTMGSAKTLPFFLIGLFTY
                     GLTAFVSSPLSSYVTAARGQLPVATVLSLTTATFGMGMVLGPVTSGWIGEHYGMRMSF
                     YAAAIVFVFSNIFVYFIGSQPIDRHDPSAPPLGLGSNKKFVTLMFVLAFAIFSMYLAQ
                     PLTPNFLEGVRGLSLSQTGLIFTIGALGNSLMAITFSRVNPRYGFLAAQSLVILFAVL
                     IWYGTSLPFFALGYFLLGGFRAGRPMALAQARELVHESQMGVTYGVMETVSAIITIIT
                     PPIAGFLFEFDPFIVYPISIGLLVLSILLSSVFLPRKESHA"
     gene            2069..2704
                     /locus_tag="PLR93_06890"
     CDS             2069..2704
                     /locus_tag="PLR93_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH26727.1"
                     /translation="MLEIVQFTLGPASTNAYLVADPETKEAVVIDPSWDGHLILAEAQ
                     KRGWRIGHLWYTHAHFDHIGGAAAIADAMNPLPHVALHPDDHVLWREGGGGKKFGLDI
                     DPGPEPTIDLIHGMTMKLGSNDFQVRFTPGHTRGHCVLYVAKENICFCGDLIFNESVG
                     RTDLPGSSFSALEKSIREQIYTMPDETVLLSGHGPKTTVGYEKKNNPFVSE"
     gene            2723..3613
                     /locus_tag="PLR93_06895"
     CDS             2723..3613
                     /locus_tag="PLR93_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26728.1"
                     /translation="MKRAVGISLGSSKRDKKVIVNLNGVEIQVERIGTDGDIEKARRL
                     YLELDGKVDAFGVGGVDLYLRLDAREYPLHAALKLVSGVKQTPLCDGRGLKHTLERRV
                     FQLAKAELGEVRFKQAFIPVAVDRMGLAEAVAEVSDQIVSGDLMVALGVPIPLHGIPA
                     FKRVARVMLPMVSYFPMSMIFYGSDGAEQEPKYVKYFEESDLIAGDFLFMRKYMPKSL
                     AGKTVVTNTTTEENIALLKERGVKTVITTTPRYDGRSFGTNTTEAMLTAYADKGRRLT
                     DEELNGLIDELGLKPSVISL"
BASE COUNT          879 a          851 c          915 g          999 t
ORIGIN      
        1 tgcatctttc gaatgtatat gcgcgtgaag agtttcggca tacttctttt gtttcggcag
       61 tgtgcaaagg aaaagtgacc ggctttggct ggcgttcata tgagttgggc ttgcgcgggc
      121 tggttgatat aatgagggcg tgaacaccca aatcattcct acaactgaac ctttttttct
      181 tcctggcggg cgaaccggtg tattgctgat tcacggcttc acgggcggac ccaaggaaat
      241 gcgctggatg ggggattatc tcaatcaaca aggatatacc tgcttaggta tccgtctcgc
      301 ggggcatgcg acaacacccg aagatatgat ccgctcgcgt tggacggatt ggacgacatc
      361 tgtagaagat gggtataact tgttacgtgg cgcaacagac aaaatctttt tagtggggct
      421 ttctatgggc ggcgtgttgt cgttgttaat gtccactcgt ttggatgtgg cgggtgtgtt
      481 ggcaatgtcc acgccgtatc aactgccgcg cgatgaaaaa gattattcgc cggaattcat
      541 tgagttttac agtcgcttcg taaagttcat gccgaaatca accgaagtgc cgggcgcttc
      601 ctggttcgat aaagaagcat tccagagcca tatttcgtat ccacaaaatc ccattcgttc
      661 gatcgcagaa atgaaacggc tcatccttgt gatgcgggcg gcattgccca atgtgaccaa
      721 gcccgtattg cttattcact caaagaatga caagtatgtt ctgcccgaaa atgtggagcg
      781 gttgtacgat ggactggtca atgcatcaga caagacaaaa gcctatgtgg tcgaggcagg
      841 tcacgttgtg acaagggatg cggctcgtca tcaagtcttt gaaatggcgc gggattttat
      901 ccgacgagta gatgcgtctc actcgtaaga ttttcaaaag aggaatcgtt gaatcgtaat
      961 ctttctttca ttgctgcggc attgctgtta tggggatttg gcgaaggcat gtttttcaat
     1021 tttgtgccga tttatttgga tgtgcaattt ttgttaagta aatacgaaat tggactcatt
     1081 cttggcgggt tcggcttttt catggcgatc acgcacatcc cggctggaag gttggctgac
     1141 cgcctgggta gaaaaccttt gcttattgtt gcttggctgt tgggtctact ttcaactctt
     1201 acaatgggaa gcgcaaagac gctgcccttt ttcttaatcg gtttatttac atatggtctg
     1261 acagcttttg tgtcatcacc attaagcagc tatgtaaccg ctgcacgcgg acagttgcct
     1321 gttgcaaccg tgctttcact caccacagct acattcggaa tgggcatggt tctaggaccc
     1381 gtaaccagtg gttggatcgg cgaacactat ggaatgcgta tgagctttta tgcggctgcc
     1441 attgtgtttg tattttccaa cattttcgtg tattttattg gaagccagcc catcgaccgt
     1501 catgacccat ctgctcctcc gctaggtttg ggcagcaaca aaaaatttgt caccttaatg
     1561 ttcgttctgg catttgcgat tttctccatg taccttgcgc aaccgctcac gcctaatttt
     1621 ctagaaggcg tgcgcggact ttcactcagc cagacgggtc ttatttttac cattggcgca
     1681 ctcggaaatt cattgatggc gatcacgttc agccgtgtga atccgcggta cggattttta
     1741 gcagcacagt cgttggtcat tctatttgcg gttctgatct ggtatggaac ttctcttccg
     1801 ttctttgcgt tagggtattt cttgttgggt ggttttcgag cggggcgccc catggcattg
     1861 gcacaagcgc gcgagcttgt ccatgaatca cagatgggtg tcacttatgg cgtgatggaa
     1921 actgtcagcg cgatcatcac aatcattaca ccgccaattg caggcttttt attcgaattt
     1981 gaccctttca tcgtttaccc catctccatt gggttattgg tcttgtctat tcttctaagc
     2041 tctgtatttc tacctcgaaa ggaatcccat gcttgagatc gttcaattca ctcttggtcc
     2101 cgcatctaca aatgcctatc ttgtagcaga ccccgaaacc aaagaagccg tggtcataga
     2161 cccctcttgg gatggacatt tgatcctcgc cgaagcacaa aaacgcggat ggcgtattgg
     2221 gcatctttgg tacacacatg cccacttcga tcatattggc ggggcagcag ctattgccga
     2281 tgcaatgaat ccgctcccac atgtggcgct tcaccccgat gaccacgtac tgtggcgcga
     2341 agggggcggc ggtaaaaaat ttggtttgga tatagacccc ggtcccgaac cgaccattga
     2401 cctgatccac ggcatgacca tgaaactcgg ctcgaacgac ttccaagtcc gcttcacacc
     2461 cggtcacacc cgcggacatt gtgtattgta tgtagcaaag gaaaatatct gcttctgtgg
     2521 cgatttgatt ttcaacgaaa gcgtcggtcg caccgatctt ccggggagca gtttttcagc
     2581 gcttgagaaa agtatccgcg aacaaatcta caccatgccc gatgaaactg tcttattatc
     2641 gggtcatggt ccgaaaacca cagtagggta tgaaaagaaa aataatccgt ttgtgagtga
     2701 ataaaaaaag aaagagtgtg ccatgaaacg tgcagtgggt atcagccttg gaagttcaaa
     2761 gcgcgataaa aaagtcatcg tcaacctcaa cggtgtggaa atccaagtcg agcgaattgg
     2821 caccgatggc gatattgaaa aagcccgcag actctatctc gaattggatg gaaaagtgga
     2881 tgcctttggt gttggtggtg tggaccttta cctacgtttg gatgcaagag aatacccgct
     2941 tcacgccgcg ctcaagcttg tctctggcgt caaacagact ccactctgtg acgggcgcgg
     3001 actcaaacac acccttgaac gccgagtctt ccaattagcc aaagccgaat taggcgaagt
     3061 gcgcttcaag caagctttca tccctgtggc agtagaccgt atggggttgg cagaagctgt
     3121 tgctgaagtc tctgaccaga tcgtctctgg tgatttgatg gtggcattgg gtgtgcccat
     3181 tccattacac ggaatccctg cttttaagcg agtagcacga gtcatgttgc cgatggtcag
     3241 ctattttccc atgtccatga tcttttatgg cagtgatggc gctgaacaag aaccgaagta
     3301 tgtcaaatac tttgaagaat ctgacttaat cgccggtgat ttcctcttta tgcgcaaata
     3361 catgcccaaa agcttggcag gtaaaactgt cgtgacaaat acaacaaccg aggaaaatat
     3421 cgccctcctc aaagaaagag gcgtcaaaac tgtcatcacc actaccccac gttacgacgg
     3481 tcgctccttt ggcacaaaca ccactgaagc catgctcact gcctatgcgg acaagggacg
     3541 caggctaacg gacgaggaat tgaacgggct gattgatgag ttagggctga agccaagcgt
     3601 gatttcttta taacaaggag caatgtcacc tgtattttga ttga
//
LOCUS       DAOQFT010000230         3643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14401_length_3643_cov_5.503623, whole genome shotgun sequence.
ACCESSION   DAOQFT010000230 DAOQFT010000000
VERSION     DAOQFT010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14401_length_3643_cov_5.503623"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1161)
                     /locus_tag="PLR93_06900"
     CDS             complement(61..1161)
                     /locus_tag="PLR93_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNH26729.1"
                     /translation="MSDLSHAYAVIMAGGGGTRLWPLSRKDKPKQLLPLIGQETLFQS
                     TVLRLKELFPPERILVVTIEEQAQEMKQQVPELPEENYLIEPAPRGTASVVGLAAMAL
                     QKRDPEASMSILPSDHFIRNVDLFHYLLKAAFDVAADNYLVTLGITPTMPSTGYGYIQ
                     QGTPIGGGYKYPVYTVQSFKEKPDEATAQQLLRMGGHSWNSGMFIWRADAILNEIDKQ
                     MPNLGKALREIGDAWNSSHKIETLKKIWPDLKNETVDYGIMEKAEKVAVLPAGGLGWS
                     DVGSWSSLFEVLLPDMSGNIATNDALHLALETNNTMVYGRGSDRLVVTIGVDDTVVVD
                     TGDVLLICKTDQSQKVKDVVEHLKKHRQEKYL"
     gene            complement(1172..2278)
                     /gene="dprA"
                     /locus_tag="PLR93_06905"
     CDS             complement(1172..2278)
                     /gene="dprA"
                     /locus_tag="PLR93_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNH26730.1"
                     /translation="MDDKKYWIGFNLIKGIGAVRMQGLVAYFGDLESAWNADAPELME
                     AGLGAKLAEKVIGARKQIDLNQVWSKIEAQGIKILTWADEEYPNRLKEIDQPPPVLYI
                     RGEYLMDDLFAVAIVGTRKVTAYGRQVTEEIASFLAANGITVVSGLARGVDAIAHQTA
                     LKAGGRTIGILGSGVDRIYPPEHRGLAEQMMQRGAIISDYAVGTPPDASNFPPRNRII
                     SGLSLAVVVIEAAETSGALITAEFAAEQGREIFAVPGSILAPQSKGTNRLIQKGAQPL
                     LTPADLMQALDLTRMDKQKSARKILPSDETEARVLNTLSSEPLHVDEIRNLANLPIEK
                     ISAALAMMELKGMVRQVGGMNYVAVRDGQSDYMA"
     gene            complement(2316..2858)
                     /gene="rimM"
                     /locus_tag="PLR93_06910"
     CDS             complement(2316..2858)
                     /gene="rimM"
                     /locus_tag="PLR93_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNH26731.1"
                     /translation="MTTEQNSGSPKGESIYLAIGYLRRPHGVQGEVIMDLHTDFPDRI
                     KSGRQVYIGEKYEAFTIGSARVHGNGLLVKLRGFDSPETAGRFRNQWVYVKSTEVPAL
                     PEGQHYKHELIGLTVMTDDDTKLGLLNEVLETGANDVYVIVKEDGKEILLPAIPDVVL
                     NINMADKVMRVHLIDGLLDT"
     gene            complement(2858..3091)
                     /locus_tag="PLR93_06915"
     CDS             complement(2858..3091)
                     /locus_tag="PLR93_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNH26732.1"
                     /translation="MKELIEYIAKSLVDHPEEVIVTETKGNRPRLGLTVAKEDMGRVI
                     GKGGKVANAIRTLLRVAAERQGKQATLDVTEPS"
     gene            complement(3166..3507)
                     /gene="rpsP"
                     /locus_tag="PLR93_06920"
     CDS             complement(3166..3507)
                     /gene="rpsP"
                     /locus_tag="PLR93_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNH26733.1"
                     /translation="MVRIRLRRIGLKGQPTYRIVAADKEAPRDGRFLEILGVYNPRTN
                     PATIKLNEDRIYHWMKNGAQPTESAAQVLKTAGTLERFERFKKGEAVEALVAEAAAAE
                     AKRAAPVKTRK"
BASE COUNT          900 a          940 c          825 g          978 t
ORIGIN      
        1 gggatggaca ttctacaaca agtttccgct tttttgcaaa cgccaatttt tcgggcgatt
       61 ttagaggtac ttttcttggc gatgtttttt gagatgttcc actacatctt taaccttttg
      121 tgactggtct gtcttgcata tcaacagcac atcgccagta tctacaacca ctgtatcgtc
      181 cactccaatg gtcacaacga ggcgatcaga tccacgtccg tagaccattg tgttgttggt
      241 ttcaagcgct aaatgcaaag catcattggt ggcaatattg ccactcatat caggcagaag
      301 gacttcgaac aacgaagacc atgaaccaac atcgctccac cccaaaccgc cagcaggcaa
      361 gacggcaact ttttcggctt tttccataat gccatagtcc acagtttcat tcttcaggtc
      421 gggccagatc tttttcaagg tctcaatctt atgagaagaa ttccacgcat caccaatttc
      481 acgcagggct ttacctaaat taggcatttg tttgtcaatc tcattcaaga tcgcatcagc
      541 acgccaaata aacataccgc tattccaaga atgcccaccc atacgaagca attgttgcgc
      601 agtggcttca tctggttttt ccttgaaact ttgcactgta taaacagggt atttgtaacc
      661 accgccaata ggcgtccctt gctgaatgta gccatacccg gtagaaggca tggtcggcgt
      721 aatgccaagt gtgaccaggt aattatccgc tgcaacatca aaagcagctt taagtagata
      781 gtggaacaaa tctacattgc gaatgaaatg gtcagacggc aaaattgaca tggaggcttc
      841 ggggtctcgt ttttgtaatg ccatggctgc taaccctaca actgaagccg ttccacgcgg
      901 agcaggttca atcaaataat tttcttctgg caattcagga acttgctgtt tcatttcctg
      961 tgcctgttcc tcaattgtca caaccaaaat gcgttcgggt gggaacaatt ctttcaaacg
     1021 caaaaccgta ctttgaaaaa gagtttcctg cccaatcaaa ggtaaaagct gcttgggttt
     1081 atctttgcgg gaaagaggcc acaaacgcgt cccaccaccg cctgccataa tcaccgcata
     1141 ggcatgtgat aaatctgaca tagtttttct cctaagccat gtaatcagac tgcccatcgc
     1201 ggacagctac ataattcata ccaccgactt gccgaaccat acctttcaat tccatcatag
     1261 ctaatgcggc tgaaattttc tcaattggca ggtttgccaa atttcgaatc tcatccacgt
     1321 gtagaggttc actactcaaa gtattcaaga ctcgtgcttc agtctcatca gaaggcaaaa
     1381 tcttgcgagc cgatttttgt ttgtccatgc gggttaagtc taaggcttgc atcaagtctg
     1441 ctggggtgag caaaggctgt gctccttttt ggatcaggcg attcgtgcct ttgctttgcg
     1501 gcgccaaaat gctacccggc acggcaaaga tctcacgccc ttgttctgca gcaaattcag
     1561 cagtgattag agcgccactg gtttcagcag cttcgatcac caccacagcc aatgataaac
     1621 ctgaaatgat tctatttctt ggcggaaagt ttgaagcgtc tggcggggtg ccaacagcat
     1681 agtcactgat gatcgctccg cgctgcatca tctgttcggc aagcccacga tgttctggcg
     1741 gataaattcg atctactcca gaaccaagga taccaatcgt tcgtccgccg gctttgagcg
     1801 cggtctgatg cgcgatggca tccactccac gggcgagtcc gctcacgacg gtgatgccat
     1861 ttgcggcgag gaaagaggcg atctcttccg tcacctgcct gccatatgct gttaccttgc
     1921 gcgtaccaac aatggcaact gcaaacaagt catccatcaa atattcgccg cggatgtaaa
     1981 ggacaggcgg cggttgatcg atttctttga ggcgattggg atattcttca tctgcccacg
     2041 tgaggatttt gatgccttgc gcttcgatct ttgaccaaac ttgattcagg tcaatttgct
     2101 ttctcgcccc gatcactttc tctgccaatt ttgcgcccag accagcttcc atcaattcag
     2161 gggcatctgc attccaagca gactcaaggt cgccaaaata ggcgaccagc ccctgcatgc
     2221 ggacagcgcc tatgcctttg atgagattga agccaatcca atacttttta tcgtccattg
     2281 aaacgcgatt ctaacactcc ccgcttggtt ttgttttagg tgtcgagcag accatctata
     2341 aggtgaactc gcataacctt atcagccatg ttgatattga gaactacatc tggaattgct
     2401 ggcaaaagaa tctctttgcc atcttctttg acaatgacat acacatcatt tgcgccagtt
     2461 tcaaggactt cattcaaaag accaagtttt gtatcgtcat ccgtcatcac ggtgagaccg
     2521 atcaactcgt gcttgtaatg ctgaccttca ggcagtgcag ggacttccgt cgatttaaca
     2581 tacacccact gattgcggaa acgcccagcc gtttcaggag agtcaaaccc gcgcagtttt
     2641 accagtagcc catttccatg cacacgggca gagccaatag taaaggcttc gtatttctcg
     2701 ccaatgtaaa cttggcgtcc tgatttgatg cggtctggga agtctgtatg caaatccata
     2761 ataacttcgc cttgcacccc atgcggtcgg cgcagatacc caattgctaa ataaatagac
     2821 tcgccttttg gcgagcctga attctgttcg gtcgtcatta ggagggttcc gtcacatcca
     2881 aggtggcttg ttttccctgg cgttcagccg ccacacgcag gagggtgcga atggcatttg
     2941 ccactttgcc acccttgccg atcacgcgcc ccatatcttc tttggctacg gtcagcccaa
     3001 ggcgcggacg gttgcccttg gtttcggtca caatgacctc ttcggggtga tccactaatg
     3061 atttcgcaat atattcgatc agttctttca ttttaaattt cctcatttac acattacgga
     3121 ctacgcagtc gaaattgcgt aatccgtaat gatgagtcct gcgatttact tgcgggtctt
     3181 gacgggcgca gcgcgtttgg cttcggcagc cgcggcttct gcaacgaggg cttcaacagc
     3241 ttcgcccttc ttgaagcgtt cgaaacgctc gagggtgcca gcagtcttga gcacctgggc
     3301 agcggattcg gtgggctgag cgccgttctt catccagtga tagatgcgat cttcattcaa
     3361 cttgatcgtg gcagggttgg tacggggatt gtacacaccc aaaatttcca aaaagcgacc
     3421 atcgcggggg gcttctttat cagcagcgac gatgcggtac gtgggctggc ccttgagacc
     3481 aatacgacgt aaacgaatac gaaccatctt aaacttgctc ctttagattt tgattacgtt
     3541 tcagacaggc actagagacg agagggatgt gcgggctgaa atcgcgggct gtattttacc
     3601 agagaaaaca ataaaaagac cccaatatcc ttggggtctg aga
//
LOCUS       DAOQFT010000231         3639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14422_length_3639_cov_6.679688, whole genome shotgun sequence.
ACCESSION   DAOQFT010000231 DAOQFT010000000
VERSION     DAOQFT010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14422_length_3639_cov_6.679688"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLR93_06925"
     CDS             <1..541
                     /locus_tag="PLR93_06925"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNH26734.1"
                     /translation="FIGSWLQTKILNAPTEEEAEKWEDLWVASICHTTAILMMWGMIM
                     PIIVWFSQKDRSARLKFQSMQAFLYQLIVTVAYFISYAVFCLKKKFGIAIFAASGVMA
                     DPSQTTMPAGFEVGFIIIMAIFMLFWLAWMILYPAYLITAGVAAVRTLRGHDFKYPLL
                     GKFLAKRMSPPESHEVIQA"
     gene            complement(596..1102)
                     /locus_tag="PLR93_06930"
     CDS             complement(596..1102)
                     /locus_tag="PLR93_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26735.1"
                     /translation="MEAIHSERLTLRPLGLDDARFIFELVNEPSFIQNIGDRGVRTLA
                     DAEKYIENGPLASYAKNGFGLLAVTLKETGEPIGMCGLIKRDTLEDVDIGYAFLPKFW
                     LKGYAVEAARTVMGTAKALGLKRVVAIVDPANTGSIRVLERLGMSFEKMVRLTADDIE
                     LKLFSVAL"
     gene            complement(1111..1527)
                     /locus_tag="PLR93_06935"
     CDS             complement(1111..1527)
                     /locus_tag="PLR93_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNH26736.1"
                     /translation="MSEVNDWNKKIIAEFRANGGKVGGNFEGRPLLLLHHKGAKSGQE
                     RVNPVACVQDGERYVVIASKGGAPTNPDWYYNVVANPLTTVEVGTETLKVQATVAEEP
                     ERTRLYNKMAEIMPGFNEYREKTTRVIPVIVLTPVK"
     gene            1649..2314
                     /locus_tag="PLR93_06940"
     CDS             1649..2314
                     /locus_tag="PLR93_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26737.1"
                     /translation="MFKSKRRQIITPQSEHLKLVGTLAMLWGNDQFDTPPIERNSMVM
                     GMGLHDRGYGYLDNAPIGALRDEEWEGIARRTFTMECSDVIADTIVKYHFRRLAGYGS
                     SEIRKKLHADFTEGIHSQLERHNLSKALFDRTDRITDLLDTLSFEFCFDVPAFGRVSV
                     FPRNGEETEVSIEYHVENGEINVHPWTFSVDEYRGYLIAYHAEGYPERLDPCILPYVL
                     KRK"
     gene            complement(2356..2901)
                     /locus_tag="PLR93_06945"
     CDS             complement(2356..2901)
                     /locus_tag="PLR93_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26738.1"
                     /translation="MEEQQLMEYFKFDAGDLNANRNGTLTEKQKTRLNAELREARKSR
                     TRWAYFLFFLAAIGIAIALGIWFIPDSGLGMRIGFGIGFGLVWPALYGLVGFALLPPA
                     SYTDLDLASQTGRVNIVRVESRDPDTHAVSSRYDLYIGPRRFLVDSNIGHVLIQGDEY
                     TIYYLKNSNKIVSAEFISRAK"
     gene            3155..3403
                     /locus_tag="PLR93_06950"
     CDS             3155..3403
                     /locus_tag="PLR93_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26739.1"
                     /translation="MQKPKTKFDQKWKLIRGHSMEWWSLIAEHDLKKVDKADDKLDKF
                     LTILQVKYGYTRVEARDEVNRRWTAYSRAKTIVESLAS"
BASE COUNT          949 a          944 c          773 g          973 t
ORIGIN      
        1 atttatcggg tcttggctgc aaaccaagat cctcaacgcc cccaccgaag aagaagccga
       61 aaagtgggaa gatctctggg tcgcgagcat ttgtcacacc accgccattt tgatgatgtg
      121 gggcatgatc atgccgatca tcgtctggtt ctcacaaaaa gaccgctccg cccgcctcaa
      181 gttccagtcg atgcaagcgt tcctatatca actcatcgta actgtggcat atttcatctc
      241 gtatgccgtc ttctgcttga aaaaaaaatt tggaattgct attttcgcag ccagcggtgt
      301 gatggcagac ccctctcaaa ccactatgcc cgccgggttt gaggttggtt tcatcattat
      361 catggctatc tttatgctat tctggctcgc atggatgatc ctctacccag cctacctcat
      421 caccgctggg gttgccgctg tgcgcacttt gcgcgggcac gatttcaaat atccactctt
      481 gggcaaattc cttgccaaac gaatgtctcc ccctgagtcg catgaagtga tccaagccta
      541 aacccatcca tgaatcaaaa acgggctcag acatttcgcc tgagtccgtt ttattttata
      601 aagcgacgga aaacaacttc aactcgatat catccgccgt taagcgaacc atcttctcga
      661 atgacattcc cagcctctcc aatacgcgga tcgaacctgt gttggcaggg tccacaatcg
      721 caaccactcg tttcaatccc agggcttttg ctgtgcccat cacagtccgt gccgcttcaa
      781 cagcatatcc ttttaaccaa aacttcggca aaaatgcata gccaatatcc acatcctcca
      841 aagtatcgcg cttgatcaag ccacacatgc caatcggctc tcccgtctct ttcagcgtaa
      901 cagccaacaa accgaatccg ttcttggcat agctggcaag cggaccgttc tcgatgtatt
      961 tttctgcatc cgccaaagtt cgcacaccac ggtcaccgat attttgaata aaagaaggtt
     1021 cattcaccaa ctcaaaaata aacctagcat cgtccaagcc cagcgggcgc aacgtaagcc
     1081 tttccgaatg gatcgcttcc atcagaaaat ttacttcact ggcgtcaaca cgatcacagg
     1141 aataacccgt gtcgtctttt cacgatattc gttgaaaccg ggcattatct ctgccatttt
     1201 gttatagagg cgggtgcgct cgggttcttc tgctacggta gcttgaactt tcaaggtttc
     1261 tgtgcccact tccacggtgg ttaaggggtt ggcaaccacg ttatagtacc aatcggggtt
     1321 ggtcggtgcg ccgccttttg aagcgatcac aacataacgt tcgccatctt gtacacaggc
     1381 aaccgggttc acacgttctt gtccgctctt ggcgcctttg tggtgcaaga gcaacaaagg
     1441 tctgccttca aagtttccgc ccactttccc gccattggcg cgaaactcgg caataatttt
     1501 cttgttccaa tcgtttactt cgctcatgaa aattctccta tggtgtttta tgtttcgatg
     1561 gtctctattt attttctctt actctaagag aattatttat tgatagaatc gcatatgaaa
     1621 ctttatagtc aaattttgga gatgtagtat gttcaaatca aaacgcagac agatcatcac
     1681 cccgcaatct gaacatctca aactggttgg cacattggca atgttatggg gaaatgacca
     1741 attcgatacg ccacccatcg aacgcaactc catggtgatg ggcatgggtc tgcatgaccg
     1801 cgggtatggt tatcttgata atgctcctat tggcgctcta cgcgacgagg aatgggaagg
     1861 catcgcccgc cgaaccttca ccatggaatg ttcagacgtg atcgccgata ccatcgtcaa
     1921 atatcatttt cgacgtctcg ccgggtatgg cagttctgag attcgcaaaa aattacatgc
     1981 cgattttaca gagggcatcc actctcaatt agagagacac aacctctcga aagctctctt
     2041 cgaccgcacc gaccgcatca cagatctttt ggatacgctt tcctttgaat tctgctttga
     2101 tgttcctgct tttggtaggg tatcagtctt tccgcgcaac ggcgaagaga cagaagtaag
     2161 cattgaatat catgttgaaa acggagagat caacgttcac ccgtggacat tttctgtgga
     2221 tgaatatcgc ggttatctca tcgcctatca cgctgagggc taccccgaac ggctcgatcc
     2281 atgtatcctg ccttatgtac tcaagagaaa ataaaaaagg tctcgcttga aatagcgaga
     2341 ccttttcatt aagttctatt ttgccctcga gataaattca gccgaaacaa tcttatttga
     2401 attcttgaga tagtaaatgg tgtactcatc tccttgaatt aggacgtgcc caatatttga
     2461 atccaccaaa aatctgcgag gaccaatgta taggtcatac cgagagctca cagcatgggt
     2521 atctgggtcg cgcgactcta ctctcacaat gttgactcgc ccagtttggc tggcaaggtc
     2581 caggtccgtg tatgaggcgg gcggtaaaag cgcaaagccc accagcccat atagagcggg
     2641 ccacaccagc ccaaagccaa tgccgaatcc gatccgcatt cccaaccctg aatcagggat
     2701 gaaccaaata cccagtgcaa tagcaatgcc aatagcagct agaaagaaga gaaaatatgc
     2761 ccagcgtgtt ctgctttttc tcgcttcgcg aagttcagcg ttaagcctcg tcttttgctt
     2821 ttcggtaagg gttccattac gattggcgtt caagtcaccg gcgtcaaact tgaagtattc
     2881 catcaactgt tgttcttcca tgggtgcttc tcctttgaac tatttttatc tctctcaact
     2941 taattcgcaa tggtacatta gcgtcacatc caacaattat caattctgaa aatcgttttg
     3001 aaaaatcact gcttttttat gcatcacgta gtacaatgaa tgcgaacgag tttgacaatt
     3061 ttgttttgcc tattgactgg ggaataagcc agaaacgaaa tcttgaaaac tccaactgag
     3121 tccaatcatt catatactct tttaggagcg cgttatgcaa aaaccgaaga caaagttcga
     3181 ccaaaaatgg aaactcatcc gcgggcattc catggagtgg tggagtctca tcgctgagca
     3241 cgaccttaag aaggtagata aagctgatga caagctcgat aaattcctta ccattcttca
     3301 ggtgaagtat ggctataccc gcgttgaagc acgtgacgaa gtcaaccgac gttggaccgc
     3361 ctactctcgg gcaaagacta tcgttgaatc actggcgagc taaagaaaag cggtctcaaa
     3421 cgagactgct ttttcttttt aaagctttgg ttataattcg ccttcatgaa actgcgccac
     3481 catcgaacct ggctggtact ccttgcactg atcattattg gactagcagg gtatggcgca
     3541 acggcaggca ctcccccttt tgtagaagcc ccatataacg gaacatccct accctatcaa
     3601 ggtgcaggcg gtgaccccgc gcttctggat gaactgcaa
//
LOCUS       DAOQFT010000232         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14444_length_3637_cov_5.948911, whole genome shotgun sequence.
ACCESSION   DAOQFT010000232 DAOQFT010000000
VERSION     DAOQFT010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14444_length_3637_cov_5.948911"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="sufD"
                     /locus_tag="PLR93_06955"
     CDS             <1..963
                     /gene="sufD"
                     /locus_tag="PLR93_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNH26740.1"
                     /translation="TDFRTAEKNHSDLLKKLIGQIVKPDDGKFAALASALSENGVLLY
                     VPKGVQVEQPLHSVLWGPGTDLAHFSHLLVYVEDGASVTYVHEAASPDETGHAMHAGI
                     VEIYVGKDANLRFVELQSWGRHVWNFSHERARVDRGGNLDWIFGAVGSRLTKNFSDLD
                     LIGEGAQGRMSGFYFTDGSQHLDHDTQQNHMAPHCTSDLLFKGALKGKSRSVWQGMIY
                     VAPGAQKTDGYQANRNLVLDDQARADSIPGLEILADDVRCTHGATVGKLEQEPLFYLK
                     SRGIPQKEAEQLVVEGFFDPIMQRIPFEGVRERFQQAIQIKLTA"
     gene            1098..1703
                     /locus_tag="PLR93_06960"
     CDS             1098..1703
                     /locus_tag="PLR93_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26741.1"
                     /translation="MAVKSKTKSNSKTKVVVKAKTATKKGTVKVSAARKVVSKSKTKV
                     VAKSVKPSKSVVSSIKKKPASAEKPVEVTRTINLSSQIRQPAKPVLRRSSVGLGATNS
                     NPFTFMKAPRPEYAFEVGDTVEVFCDHEKGRERVRGWIKGIVVQVDNKMVAVQFRSNV
                     FLTDGWMVPDRILWYPLNSEHIRPGTTGKKPALKKDIIPDY"
     gene            1745..3007
                     /locus_tag="PLR93_06965"
     CDS             1745..3007
                     /locus_tag="PLR93_06965"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNH26742.1"
                     /translation="MCTCVPVYFLFPMTLNINEIRKDFPILERETANGKRVIYLDSTA
                     TSQKPLQVIEAMNDYYRRSNANIHRGVHTLAEEATSLYEGARERIAKFIHAATSREII
                     YTRNTTESINLVAYSWARANLKAGDLVILTEMEHHSNLVPWHMLQAERGIELEFIPVT
                     EDGLLDLEAYKTLLNRTPKLVSFTHMSNVLGTINPAAEIIKLAHAAGAVTLVDGAQSV
                     PHLAVDVQALDADFYAFSAHKMCGPTGMGVLYGKSALLEAMPPFLGGGDMIKEVKLRS
                     FRPNTLPHKFEAGTPAIAEAIGLGAAVDYLTQIGISDIAAHEHEITEYALERLEEIPG
                     IKLFGPSADKKGGVAAFTLEGVHPHDVAQILDQDGIAVRAGHHCAQPLHEKFGIPATS
                     RASFYLYSTKDEVDMLVKGLYKVKEMFG"
     gene            3056..3451
                     /locus_tag="PLR93_06970"
     CDS             3056..3451
                     /locus_tag="PLR93_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNH26743.1"
                     /translation="MDDLYREVIIEHYKNPSYRGKLDPHDISFADNNPLCGDHIQIDL
                     RVDANGVVSEARFDGHGCAISQASADLLMETIIGKPLEEVKKLNRQDILDMLGIDLGP
                     VRLKCALLSLKVLKAGVYGLGEAGDDLVE"
     gene            3457..>3637
                     /locus_tag="PLR93_06975"
     CDS             3457..>3637
                     /locus_tag="PLR93_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26744.1"
                     /translation="MASYQIREMVIDDYDAIIVMFHETPGITVRDADSRESTAAYLMR
                     NPGLSFVATINGKIIG"
BASE COUNT          923 a          836 c          953 g          925 t
ORIGIN      
        1 actgacttcc gcacagcaga aaagaatcac tctgacttac tcaagaaatt gatcggtcag
       61 atcgtcaagc cagatgatgg caagtttgcc gccttggctt cggcactttc tgaaaatggc
      121 gttcttcttt atgtgcccaa gggcgtacaa gtggaacaac cccttcactc tgtgttgtgg
      181 ggacccggta ctgacctcgc gcatttctcg catctactcg tttacgtgga agatggcgct
      241 tcagtgacgt atgttcacga agccgcttca cccgatgaga ccggtcatgc catgcatgcg
      301 ggaattgtcg aaatctatgt agggaaagat gccaacctgc gctttgtaga attacagtcg
      361 tggggtaggc atgtgtggaa tttcagccac gaacgcgccc gtgtagatcg tggcggcaac
      421 ctcgactgga tcttcggtgc tgtaggctct cgcctcacca agaacttctc agacctagac
      481 ctgattgggg aaggggcgca gggacgaatg tctggcttct actttaccga cggaagccag
      541 cacctcgacc acgacaccca gcagaatcat atggctccgc actgcaccag cgaccttttg
      601 ttcaaaggtg cgcttaaagg aaagagccgc tcggtgtggc agggtatgat ctatgttgcc
      661 cccggtgccc agaagacgga tggctaccaa gccaaccgta acctcgtcct cgatgaccag
      721 gcacgcgcgg attctattcc gggtctcgaa attctcgccg acgatgtgcg ctgtacacat
      781 ggcgcaaccg ttggtaaact tgaacaggaa cccctgtttt acctgaaatc tcgtggtatt
      841 ccgcaaaaag aggcggagca gttggtggtg gaaggtttct ttgaccccat catgcagcgc
      901 atcccgtttg aaggtgtgcg tgaacgcttc cagcaggcga tccagattaa gttgacagcg
      961 taatttgttt tctctcgctg gggtgtacac cccagcgaga gcattatcac cgtaagaaaa
     1021 tgcctgaagc gttgcatcag gcatccttta agaatgaggc ggagggcgtc tcaattttcg
     1081 ttcgtaggaa gtgagctatg gcagtgaagt ctaaaacaaa atccaattcg aagacaaaag
     1141 tggtggtaaa agcgaaaacg gcaaccaaaa aggggacggt taaggttagt gcggctcgta
     1201 aagtagtttc aaaatcgaaa acaaaagtgg ttgcaaaaag tgtcaagcct agtaagtcgg
     1261 tcgtttcttc tataaagaag aagccagctt ctgctgaaaa accggttgaa gtgacgcgca
     1321 ccattaactt atcgagccag atccgtcagc ctgcgaaacc tgtcttgcgc cgctcttctg
     1381 ttgggttggg tgcgacgaac tccaatccgt tcacgtttat gaaggcgcca cgccctgagt
     1441 atgcttttga agtgggtgat acggtagaag tgttctgtga tcacgaaaag ggacgcgaac
     1501 gtgtgcgtgg ttggatcaaa ggcattgtgg ttcaagtgga taacaaaatg gtggcagtgc
     1561 agttccgctc gaacgttttc cttacagacg gttggatggt gccagatcgc attctttggt
     1621 acccgctgaa ttctgagcac attcgccctg gaaccaccgg caagaaaccc gccttgaaga
     1681 aagatattat tccggattat taagccaata cgcaggtaga caggtacaca agcaaaaaat
     1741 agacatgtgt acttgtgtac ctgtctattt tttatttccc atgacgctaa acataaacga
     1801 aattcgtaaa gatttcccta ttctagagcg cgaaactgcc aatggcaagc gcgtgatcta
     1861 tcttgactcc accgcaactt ctcagaagcc tttgcaagtg atcgaagcga tgaacgatta
     1921 ttatcgccgt tcaaatgcaa atattcaccg tggtgtgcat actttggctg aagaagccac
     1981 ttctttgtat gaaggtgcgc gtgaacgcat tgccaaattt atccatgcgg caacttcacg
     2041 tgagattatt tacacgcgta atacaaccga gtcgattaat cttgtcgctt attcttgggc
     2101 gcgtgcaaat ttaaaggcgg gcgaccttgt tattctgacc gagatggaac accatagcaa
     2161 tctggttccg tggcatatgc tgcaagcaga gcgtgggatt gagctggaat tcatccctgt
     2221 gacagaagac ggtttgctgg atctggaagc ttataaaacg cttctcaacc ggaccccgaa
     2281 actggtctcg tttacacaca tgtccaacgt gttgggcacg atcaacccgg cggcagagat
     2341 catcaaatta gctcatgcgg cgggtgctgt cactctagtg gatggcgctc aatcagtccc
     2401 tcacttagcc gtggatgttc aagccttgga tgctgatttt tatgcgttct ctgcgcataa
     2461 gatgtgtggt cccaccggga tgggtgtgtt gtatggcaag tcagctttgt tagaagccat
     2521 gcctccgttc cttggtggtg gtgacatgat caaggaagta aaactgcgtt ccttccgccc
     2581 gaacactttg ccacataaat ttgaagcggg tacacctgcc atcgcagaag ccatcggttt
     2641 aggtgctgct gttgattatt tgacccagat cggcataagc gatattgctg cccatgagca
     2701 tgagatcact gaatatgcgc ttgaacgctt ggaagaaatc cctggtatta agttgtttgg
     2761 tccctctgcg gataaaaaag gtggcgtagc tgcttttaca cttgaaggcg tgcaccccca
     2821 tgatgtggcg caaattttgg atcaggatgg catcgccgtc cgcgcggggc atcactgtgc
     2881 tcagcctttg catgaaaagt ttggcatccc tgctaccagc cgagcttctt tctatttgta
     2941 ctctacaaaa gatgaagtcg atatgttggt aaagggttta tataaagtga aagaaatgtt
     3001 cgggtaatta tagggcgagg tttccttgcc ctgtttccct gaaacggaat atataatgga
     3061 cgatctctat cgtgaagtaa tcattgagca ctataaaaat ccatcttatc gcggaaagtt
     3121 agacccgcac gatatttcct ttgcagataa caacccatta tgcggcgacc atatccaaat
     3181 cgatttgcga gtggatgcga atggtgttgt cagcgaggca cggtttgatg ggcatggttg
     3241 tgcgatctct caagcctcag cagacctgtt aatggagact attatcggga agccgctgga
     3301 agaagtaaaa aagctcaacc ggcaggatat tcttgatatg ctcggtattg acctcggacc
     3361 ggtgcgtttg aagtgtgcat tgctttcgct taaagtgttg aaggctggag tatatggctt
     3421 aggggaagcc ggagacgatt tggtggagta agaagcgtgg cttcatatca gatccgcgag
     3481 atggtcattg atgattacga tgccatcatt gtgatgtttc acgagacgcc gggcatcacg
     3541 gtacgagacg cagactcgcg agaatcaaca gccgcttatt tgatgagaaa ccccgggctg
     3601 agcttcgtag ccaccataaa tggaaagatt atcggct
//
LOCUS       DAOQFT010000233         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14473_length_3632_cov_8.304166, whole genome shotgun sequence.
ACCESSION   DAOQFT010000233 DAOQFT010000000
VERSION     DAOQFT010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14473_length_3632_cov_8.304166"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>3632
                     /locus_tag="PLR93_06980"
     CDS             44..>3632
                     /locus_tag="PLR93_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26745.1"
                     /translation="MNLSAVLDKIAKHARDLLKGSSSAIYLPEPDGKSMRALVAQGDI
                     AEEILADSIQVGEGIIGSLAKLGKAEYINDTNHDPRTLQIPGTPAVQNERLLVTPLLS
                     GEKVNGMMAVWRKGGEPFSPADQRFQEELSLQAAIAIQNANFVNEVEQRAHELEIINS
                     IQQGLSTKLDVQSVYELVGEKVRSIFNSQAVIISYYHPETETADFPYMYWKGERIYPG
                     REPLSGFSGYVIRNKAPIFINSNLEEMAHSYGSTLLAGDTFPKSLIAIPILSGDQVFG
                     SLSIQNFEQERAFTENDLRLLSTLAASMGISLQNAKLFDESQRLLVETRQRAEELALI
                     NTILSGLDTKLGIQTVYDATGEKIREIFDAQTVVLSIYDKENQLTYYPYIIENGERLY
                     QDPLPLREDGGGFGGYVIRTKEPLLVNRDFEEYSKRFQSANLGVDAEVEVVVRAGLWV
                     PMLVGDEVKGVISLQNLEREDAFSDSDVRLLSTIANSIGVAIENIRLFDETQRLLKET
                     EQRATELAILNNVSESMSRALDVNAVTHTVGNKIHEIFSAEIVDILLYNSATNIVSLV
                     FSFSNNLSYENEPPWELGEGLTSKIIITKQPLLLNTAKEIEDNGAATYVTAPEDEEDI
                     KSYMGVPIMVGDQVLGVVDVQSYKAHAFDEGSLRILQILSANMGVAIQNARLFDETQR
                     LLKETEQQNSDLSALNSVQQALVSNIDVKSIYQAVGRKLTALFNVHSAVIYTVDLDTY
                     SMTYEYAYEQGKEWDIPPKQATSLHVYITDTVKTNRKSLVINKNFDEFAAQFPDFKTS
                     RSRFPKSLCAVPILIRKNSVTGISLQHLDTEDFFNDTSLRLLETIASATSVALENARL
                     FDETQRLLNETKRRATELEILNNIGQVLTQQLDVNSIIEMVGDKVRELVNEDNLGIGL
                     YDEETKTVTAHYVTKGSERIQFPPFPINEFTQRAALQGKSLVINRRSPILWKKLGSHM
                     TAMDEIPKSVVMVPMIVGKELIGGFTIQNFEHENAYDESIITLLESIASGTATAIQNA
                     RLFEEARLARAAAEQANEAKSAFLATMSHEIRTPMNAVIGMSGLLLDTELNDEQRDYV
                     ETIRGSSDTLLAIINDILDFSKIEAGRMDLEHQPFDLRECVEASLDLVTARAVEKSID
                     IAYIFEGDIPPAIVGDVTRLRQVITNLLSNAVKFTDRGEVVLTVSSQRYK"
BASE COUNT          982 a          925 c          869 g          856 t
ORIGIN      
        1 gcttggcgat cttatctaac accgctgaga tatatccgcc acattgaatc tctcagcggt
       61 gttagataag atcgccaagc acgcccgcga cttattgaaa ggctcaagca gtgctattta
      121 tttgcccgag ccagatggca agtcgatgcg ggcgttggta gcacaagggg atattgcaga
      181 agaaattctg gcagattcca ttcaggttgg ggaaggcatt atcgggtcgc ttgccaaact
      241 cggcaaggca gaatacatca acgataccaa tcacgacccg cgcacgttac aaatccctgg
      301 cacacctgcg gttcaaaatg aacgcttgtt ggtcactccg ctgctttctg gtgagaaggt
      361 caacggtatg atggcggttt ggcgcaaagg cggcgaacct ttctcccctg cagaccagcg
      421 ttttcaagaa gagttgtctc tgcaagctgc gattgccatt caaaatgcga acttcgtcaa
      481 tgaagtggaa caacgtgccc acgaattgga gattatcaat agcatccaac aagggttatc
      541 gaccaaatta gatgtacagt cggtttatga actggttgga gagaaagttc gcagcatctt
      601 caactcgcaa gctgtgatca tctcgtatta tcaccccgaa acagaaactg ccgatttccc
      661 ttacatgtat tggaaggggg aacgcatcta ccctgggcgt gagccgcttt ctggtttctc
      721 tggctatgtc attcgtaaca aagcgcccat ctttatcaac tcgaacttgg aagaaatggc
      781 acacagttac gggtctactc ttttggctgg ggataccttt ccaaaatcgt tgatcgctat
      841 tcccattctc tccggtgacc aggtcttcgg ttcgctttcc attcaaaact ttgaacaaga
      901 acgcgccttt acagaaaatg acctgcgttt gctttcgacc cttgcagcaa gcatggggat
      961 ttcgcttcag aatgccaagc tattcgatga gtcgcaaaga ttgttggtcg aaacccgtca
     1021 acgcgcagaa gaattggcgt tgatcaacac catcttaagc gggttggata ccaaacttgg
     1081 cattcaaacg gtatacgacg caactggtga aaaaatacga gagatcttcg atgcacagac
     1141 cgttgtgctt agcatctacg ataaagaaaa tcaactcacc tattatccat acattatcga
     1201 gaatggtgaa cggctctatc aagacccctt accgcttcgc gaagatggag gcgggttcgg
     1261 tggatacgtc atccgcacca aagagccgct attggtcaac cgcgatttcg aagaatactc
     1321 caaacgcttc caatcggcaa accttggtgt ggatgccgaa gtagaagtag tggtgcgtgc
     1381 cggactgtgg gtgcctatgt tggtcggcga cgaagtgaaa ggcgtgatct cgctgcaaaa
     1441 ccttgagcga gaagatgcct ttagcgactc agatgtgcgc ttgctttcaa cgattgccaa
     1501 ctcgatcggg gtggcgattg agaacattcg cctcttcgac gaaactcagc gcttgctcaa
     1561 agagaccgag cagcgtgcca ccgaacttgc cattctcaac aatgtcagcg agtcgatgtc
     1621 gcgcgcgttg gatgtaaacg ccgtcactca caccgtgggc aacaagatcc acgagatctt
     1681 cagtgccgag atcgtggaca ttctgcttta taactcagcc acgaatatcg tgagtttggt
     1741 attcagcttt agcaacaatc tttcttatga gaatgaacca ccttgggaat tgggagaagg
     1801 gctcacctcc aagatcatta tcacaaaaca gccgttgttg ttaaacactg ccaaagaaat
     1861 cgaagacaac ggtgcagcga cctatgtcac cgcccctgaa gatgaagagg atattaaatc
     1921 ttatatgggt gtgcccatta tggtgggcga ccaagtatta ggcgttgtag atgtccaaag
     1981 ctataaagct catgccttcg acgaaggcag tttgcgcatt ctacaaatcc tttctgccaa
     2041 tatgggcgtt gccattcaaa atgcacgctt attcgatgaa acccagcgcc tcttaaaaga
     2101 aaccgaacag cagaattccg acctttcggc tctcaactcg gtacagcaag cgttggtctc
     2161 caacattgac gtgaagtcga tttatcaagc ggtagggcga aaactaaccg cgctcttcaa
     2221 cgtccattcg gcggttatct atacggtaga ccttgatacg tatagtatga cctatgaata
     2281 tgcctacgaa caaggcaagg aatgggacat tccgcccaaa caggctacca gtttgcatgt
     2341 gtacatcaca gacactgtaa aaaccaatcg caagagtctg gtgatcaata aaaactttga
     2401 tgagtttgcc gctcaattcc cagatttcaa aacctcgcgc agccgtttcc ccaagtcgct
     2461 atgtgcggta ccgatcttga tccgcaaaaa ttcggtgaca gggatttcct tgcagcatct
     2521 tgatacagaa gatttcttca acgacacttc gctacgtctg ttggaaacca ttgccagcgc
     2581 caccagtgtg gcactcgaaa atgcacgcct ctttgacgaa acccaacgcc tcttaaacga
     2641 gaccaagcgc cgtgccaccg agttggaaat tctcaacaac atcgggcaag tgctcaccca
     2701 acaacttgac gtcaacagca ttattgaaat ggtcggcgac aaagtgcggg aactcgtcaa
     2761 tgaagataat ttaggcatcg gtttatacga cgaggaaacg aagactgtca cagcccatta
     2821 cgtaaccaaa ggcagcgagc ggattcaatt cccgccgttc ccgatcaacg agttcaccca
     2881 aagagccgcg ttgcaaggca agagtctggt catcaaccgc cgctccccca ttttgtggaa
     2941 gaagcttggt tcccatatga ccgccatgga tgaaatcccc aaatcggttg taatggtgcc
     3001 aatgatcgtg ggcaaagaac tgattggtgg tttcaccatc cagaactttg agcatgagaa
     3061 tgcctatgac gaatccatca ttaccttgct cgagtccatt gcttctggta cagccacagc
     3121 cattcaaaat gcacgcctgt tcgaagaagc gcgtttggca cgtgccgcgg cagaacaagc
     3181 caacgaagcc aagagcgcct tcctcgcgac catgagccat gaaattcgca cgcccatgaa
     3241 tgccgttatc ggcatgagcg gacttttgct cgataccgaa ctcaacgacg aacagcgtga
     3301 ttatgttgag accattcgcg gctcaagcga taccttgctt gccatcatca acgacattct
     3361 cgacttctcg aagatcgaag ccgggcgcat ggaccttgaa catcagccct ttgacctgcg
     3421 tgaatgtgtc gaagcatcgc tagacctggt cacggcgcgg gctgtagaaa aaagcatcga
     3481 cattgcatat atcttcgaag gcgatattcc gcccgccatt gtgggcgatg tgactcgctt
     3541 acggcaggtc atcaccaatt tgctcagcaa cgcggtcaag tttacagaca gaggcgaggt
     3601 cgttttaacg gtatccagcc aacggtacaa ga
//
LOCUS       DAOQFT010000234         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14475_length_3632_cov_5.539279, whole genome shotgun sequence.
ACCESSION   DAOQFT010000234 DAOQFT010000000
VERSION     DAOQFT010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14475_length_3632_cov_5.539279"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLR93_06985"
     CDS             <1..347
                     /locus_tag="PLR93_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNH26746.1"
                     /translation="PKPTIRIEGIGTEVQTFFMGEAQFTLNGKDYSLQAEESGDELLF
                     NFKDETNTDTTYGGGRRFYLLPPEGDELILDFNLTDNWPCAYTPFATCPIPPKENTLK
                     VRIEAGEKKFKK"
     gene            complement(381..941)
                     /locus_tag="PLR93_06990"
     CDS             complement(381..941)
                     /locus_tag="PLR93_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNH26747.1"
                     /translation="MELFEAIHGRTTVSKVKQDALSRETIEKLLSAGAQAPNHHKVRP
                     WRFVVLTGDGRKKLGDVMTASFADRNPGAPAEGLDKTRNLPLRAPVVIAVGADKPADA
                     KIQEIENISAASAACQNILLAAHALGLGAIWRTGEWARDVKVKEFLGFAPDQHIVGFI
                     YIGNPDIMPDPYTRIGFEDRVTWIDK"
     gene            989..2029
                     /locus_tag="PLR93_06995"
     CDS             989..2029
                     /locus_tag="PLR93_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNH26748.1"
                     /translation="MKIFYSEEHRNHYPPFEVFDGGTRVQYFENPDRMDQILNALQGE
                     NWVTFLEPDDFGLDPILAVHDRDYLNFLASCWDEWLASDPEVAASPETHAFLPATFAL
                     RRKNARPTSSIRGRGGYYIMDLSASIVAGTYKAALTSANIALSAASAVYGGDKSAFAL
                     CRPPGHHAGKDYAGGYCFINNAAVAANWLANKGKVAILDVDYHAGNGTQDIFYDRNDV
                     LSISIHGDPDFEYPHYVGFAEETGTGAGLGFHRNFPLPKGTKDEEYLSALDEALNLIR
                     DYAPQYLVVSFGADAFDGDPLGLFKLTRNGFSEIGKRIAGLNLPTAIIMEGGYANAAL
                     GDNTKTLLENFK"
     gene            2026..2454
                     /locus_tag="PLR93_07000"
     CDS             2026..2454
                     /locus_tag="PLR93_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26749.1"
                     /translation="MKKWHSLLFVFVFTLTACGAAATEPPPPTETSTPIIVYITATSQ
                     PTLTPTITFTPGPTNTVEPSVTPTPEFSFPTVTVNKQAHCRYGPSAAFLHAADLYAGD
                     TGTVRNRAMYSNWLYIKFDKLNYFCWVHHLSWMWSEMSLH"
     gene            2424..2783
                     /locus_tag="PLR93_07005"
     CDS             2424..2783
                     /locus_tag="PLR93_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNH26750.1"
                     /translation="MDVVGDVTTLKRYEPNLQSIGSNQYGPPQNVQATRNGDEVTITW
                     DQMEMSDDKDRGYLIEAFVCQGGAYIWWTFSYPDQFSTSYTVKDEAGCASPSYGKIYT
                     VEKHGFSYPADIPWPAP"
     gene            complement(2832..3200)
                     /locus_tag="PLR93_07010"
     CDS             complement(2832..3200)
                     /locus_tag="PLR93_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26751.1"
                     /translation="MTEGLIIDDNRQTADALQQMLGLLDLPARVAYGSSAAMSVLASI
                     VPQFICLDINMPGVDGTEVLAYIRREPRLMKVPVIIITSDDQPETRQHVMRGGAQAMI
                     IKPVTIEILEGAFKKAGIIE"
     gene            complement(3390..>3632)
                     /locus_tag="PLR93_07015"
     CDS             complement(3390..>3632)
                     /locus_tag="PLR93_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH26752.1"
                     /translation="SFVGDVRGLGLVCGVEIVSDKTTKTADPATTMKIFKAAEALGLR
                     SRPLGNTLAFSPPLSITEDEVDEIVKRLGAAMDGVA"
BASE COUNT          945 a          932 c          833 g          922 t
ORIGIN      
        1 cgcccaagcc aacgattcga atagaaggca ttggtaccga agtgcaaact ttctttatgg
       61 gagaagctca attcaccttg aacggaaaag actattcact tcaagccgaa gaaagtggcg
      121 acgaattgct ctttaatttc aaggatgaaa ccaatacaga tacgacttat ggtggtggac
      181 gcaggtttta tcttctgccg ccagaaggcg atgaactcat tttagatttc aacttaactg
      241 acaattggcc ctgcgcgtac acaccttttg ccacctgccc tattccgccc aaagaaaaca
      301 cattgaaggt ccgcatagaa gctggcgaga agaaatttaa gaagtaaaca aaaagcgagt
      361 ggcaagccac tcgctttttg ttatttatca atccatgtta cacgatcttc aaacccaatt
      421 ctagtatatg ggtctggcat tatgtctggg ttcccaatat aaataaaacc aacaatatgc
      481 tgatcgggcg caaagccaag gaattctttt actttcacat cgcgtgccca ttcgccggtg
      541 cgccaaatag cgcccaagcc taaagcatgt gccgctaaca aaatattctg gcaagctgct
      601 gaagccgcag aaatattttc aatttcttgt atctttgcat cagcaggttt atctgcacca
      661 acggcaatta caaccggtgc gcggagcggc aagtttcggg ttttatccaa gccttcggct
      721 ggcgcacccg ggttccggtc ggcgaaagag gcggtcatta catcgcctaa cttcttacgc
      781 ccatccccgg ttaacacaac aaaacgccag ggtctcactt tgtgatgatt cggcgcttgt
      841 gctccggcac ttaataattt ttcaatggtc tcgcgtgaaa gtgcatcttg cttcactttg
      901 ctgaccgttg ttcgtccgtg aattgcttca aaaagttcca tttcgatacc tccgggagtg
      961 agacgcttgc aggtaaaatt gtcttaccat gaaaatattt tactcagaag aacaccgcaa
     1021 tcattatcca cctttcgaag tgttcgatgg cggcacaagg gttcagtatt ttgaaaaccc
     1081 agaccgcatg gatcaaatct taaatgcgct acagggtgaa aactgggtga catttttaga
     1141 gccagatgat tttggactcg acccgatcct tgctgtgcac gaccgtgact acctcaactt
     1201 cctcgcctcc tgctgggatg agtggctggc atctgacccc gaagttgctg cttcgccaga
     1261 aacgcatgct ttcttgcccg ctacctttgc tttgcgaaga aaaaatgcac gccccacctc
     1321 ctccattcgc ggacgtggcg ggtattacat tatggacctt tctgcttcca ttgtagcagg
     1381 gacgtataaa gccgcgctga cttcagcaaa tattgccttg agcgccgcct cagcagttta
     1441 tggcggagat aagtcggctt ttgcgctttg ccgtccgcca ggccaccatg caggcaaaga
     1501 ttacgcgggc ggatactgct ttatcaacaa tgcagcggtg gctgcaaact ggctggcaaa
     1561 taaaggcaaa gttgccatat tagatgttga ctatcatgca gggaacggca cccaagatat
     1621 tttctatgac cgcaatgacg ttctcagcat ctcgattcat ggcgacccag attttgaata
     1681 tccgcactat gtaggtttcg ctgaagaaac agggactgga gccgggcttg ggtttcaccg
     1741 aaactttcct ttacccaaag gcacaaaaga tgaagagtat ttatcggctt tggacgaagc
     1801 attgaacctg attcgagact atgcgccaca atatcttgtc gtctctttcg gcgcagatgc
     1861 ctttgatggt gacccactgg ggttgttcaa actgaccaga aatggtttta gcgagatcgg
     1921 caagcgaatt gctggattaa acctgcctac tgcgattatt atggaaggcg gttacgccaa
     1981 cgcagcgctc ggagacaata ccaaaacttt attggagaat ttcaaatgaa aaaatggcac
     2041 tcgcttttat tcgtctttgt ctttacccta acggcttgtg gcgcggctgc aacagaacct
     2101 cccccgccta cggaaacctc cacaccgatt attgtctaca ttactgccac gtcacaaccg
     2161 acattgacac caacgattac cttcacacct ggtccgacca atactgttga gccaagcgtt
     2221 actcctactc ctgagttcag ctttcccaca gtcacggtca acaagcaagc gcattgccgc
     2281 tacggtccgt ctgcggcatt tctccacgca gcagacctat atgctgggga tactggaacg
     2341 gtgagaaacc gggcaatgta tagcaattgg ctttacatca agttcgacaa actcaattac
     2401 ttttgttggg tgcaccatct gtcgtggatg tggtcggaga tgtcactaca ctaaagcgct
     2461 acgagccaaa tcttcaatcc attggcagca atcaatatgg tccgccgcag aacgtgcaag
     2521 ccacgcgcaa tggcgacgaa gtgaccatca cttgggatca gatggaaatg agtgatgaca
     2581 aagatcgcgg ttacttgatt gaagcctttg tttgccaagg cggcgcttac atctggtgga
     2641 ctttctcgta cccagaccag ttcagcactt cgtacacagt caaggacgaa gccggttgtg
     2701 cttcaccgtc ttacgggaaa atatatactg tagaaaaaca tgggttctct tacccggcag
     2761 atatcccctg gcccgcaccc taatccatta aatcaaaagg ctcctgtttg aaacaggagc
     2821 ctttattttt gctattcgat gatgcccgct tttttgaagg cgccttccag aatttcgatc
     2881 gtcactggct tgatgatcat tgcctgcgcc ccaccgcgca tcacatgttg acgtgtttcg
     2941 ggctggtcat cagaagtgat gataatgacc ggcactttca tcagacgtgg ctcgcggcga
     3001 atatatgcca acacctcggt cccatccaca ccgggcatat tgatatccaa acaaataaat
     3061 tgcggcacaa tacttgccaa aacagacatg gcagcgctgg agccataagc aactcttgcc
     3121 ggtaagtcca acaacccgag catttgctgt aaagcgtccg ccgtctggcg gttatcgtcg
     3181 atgattaatc cttcggtcat tagtcctcca atatggcgcg ctctaagaga cacgtcttgt
     3241 tctttttgtt tggaaatacc tctcgtttaa tccccgtcac cggtcaacca aggagaggat
     3301 gtaaggcgag tatacacttc ctgattacag cccgcaatgg gatattagaa actgtcatta
     3361 aactcaaatg atatagttaa caactgagct taggcaaccc cgtccatggc tgccccgagg
     3421 cgtttaacga tctcatctac ttcatcttcg gtaatagaga gcggaggtga aaacgccagc
     3481 gtattcccaa gcggacggga gcgcagccca agggcttcgg cggctttgaa gattttcata
     3541 gtggtagcgg ggtcggcagt tttggtggtt ttatctgaaa caatctccac cccgcagacc
     3601 aaaccaagtc cgcgcacatc accaacgaag ga
//
LOCUS       DAOQFT010000235         3604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14665_length_3604_cov_7.353057, whole genome shotgun sequence.
ACCESSION   DAOQFT010000235 DAOQFT010000000
VERSION     DAOQFT010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14665_length_3604_cov_7.353057"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLR93_07020"
     CDS             <1..491
                     /locus_tag="PLR93_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNH26753.1"
                     /translation="DTLAKLEKGIENLPDLSSLGGQLSAPFLFFNNTRSMTVIFLAGI
                     FSFSVLGVLLYMVNIALIGGVYAVLELLGIAPAPIFLAGILPHGIFELPALLIGSAVV
                     LYIGAALVTPQNGKSMGEVMIDLMADWAKIFVGVVVPLLAIAALIEAYITPSILLRVV
                     GN"
     gene            593..1348
                     /locus_tag="PLR93_07025"
     CDS             593..1348
                     /locus_tag="PLR93_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH26754.1"
                     /translation="MAKVIILGASNAIPTSESENTHMVIVGNDRTVLIDSVSNPILRL
                     EQAGLDFNNLTDIIITHFHPDHASGIPLLLMDMWLMGRQKPLNIYGLHYTLDRVEGLM
                     GFYNWSEWPNFFPVVFYRLPVREMTPVLDCPDFIIHASPVHHMIPNIGLRVNFQQSQK
                     VMAYSCDTEPCDEVVRLSEGADVLIHEATGESPGHSSATQAGEIASRAEVNKLYLIHY
                     PTGRFKKGDIAAEASQTYQGEIQIAKDFMVLDF"
     gene            complement(1382..1885)
                     /locus_tag="PLR93_07030"
     CDS             complement(1382..1885)
                     /locus_tag="PLR93_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNH26755.1"
                     /translation="MNEPVLVLNANFEPIHVCSTRRAVTLILAGKAGMIANGRGHIRT
                     ISQLIPRPSVIRLESQIHRPRPRVKLTRREIFRRDNYTCQYCGKRDTNLTIDHVIPRH
                     MGGKQVWTNLVTACSPCNHRKGGRKVDEAHMHLLHIPKEPPADASYIFGKHLAENQEW
                     TPYITGW"
     gene            1970..2863
                     /locus_tag="PLR93_07035"
     CDS             1970..2863
                     /locus_tag="PLR93_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNH26756.1"
                     /translation="MNIMISGGTGLLGTALTKSLLADGHKVFILSRTDKKRSDVQIIK
                     WDARTPDGWGHLVNEMDVVIHLAGRSTSAWPWSAANKKSFEDSRILPGLALAQAIQAA
                     TRRPSLFVQVSGINHYGLHGDLADESTPPGDDFLAQLTVKWEGATQSVERLGVRRLVL
                     RTPPVLSRDNVIMKLIALPVQLFVGGPIGSGKQPFPWIHIKDWVSAVRYLMANESSRG
                     VYNMIAPSQTSLADFTKILANVLHRPYWFPVPAFLMRNVLGEMSVLILEGRFSQPKRL
                     LESGYTFQFPGPREALTDLFG"
     gene            2886..3566
                     /locus_tag="PLR93_07040"
     CDS             2886..3566
                     /locus_tag="PLR93_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26757.1"
                     /translation="MKKMSIALLLMILISACAPAQDSQVVLPQLLPTATAYIDASSYP
                     TAEANVSAAQQTAAGIDVRMNRAWMEGKNVNAEVCFTLPDASDWSIWAANLAYSGIVL
                     QEYGTTLQSIQESANGQPGVRCDILTFVVAPDADLTNAAINIDAIAAPPQPSEYCDLY
                     LPKIQQAMLERGTGISVDCVDVNGALTMQILNKPAEMSQEQAEQLVYSDEFYTVRGPW
                     SFPFNLSQ"
BASE COUNT          891 a          859 c          857 g          997 t
ORIGIN      
        1 ccgatacgtt ggcaaagctt gaaaagggta ttgaaaacct accagacctt tccagcctgg
       61 gtggacaatt aagcgcgccc ttcttgtttt tcaacaacac ccgttccatg accgttatat
      121 ttctcgctgg gatattttcg tttagtgtct tgggcgttct gctgtatatg gtcaacattg
      181 ctttgattgg tggcgtatat gccgtgttgg aacttttagg cattgcgcct gctccgatct
      241 ttctggctgg aattttgccg catggtattt ttgaactccc ggctttgcta attggtagtg
      301 ccgtagtgtt gtacattggt gccgccttgg tcacaccgca aaatggcaag tcgatggggg
      361 aggttatgat cgatctcatg gcagattggg caaagatatt tgttggtgtg gtggtgccat
      421 tgcttgcaat cgcagcctta attgaggcat atatcacccc tagtattcta ttaagagttg
      481 tcggaaatta attcaagaaa agcgagctgt cttgtataag acagctcgct tttcttatgg
      541 ctcatatgaa aatacaatta acaattaagg ggatttgagt taagatttga ttatggcaaa
      601 agtcatcatc cttggggctt caaacgcaat tcccacaagc gaaagcgaaa acacacacat
      661 ggtcattgtg ggaaatgacc gtaccgttct gatcgattca gtgagcaatc ccattctacg
      721 gcttgagcaa gccgggctcg actttaataa ccttaccgac attatcatca cccattttca
      781 tcccgaccat gcctccggta ttccgctttt gcttatggat atgtggctga tgggcaggca
      841 aaaaccgctc aatatctatg gtctgcacta cactcttgac cgtgttgaag gcttgatggg
      901 cttttataac tggtctgagt ggccaaactt tttcccggtc gttttctatc gtttacccgt
      961 ccgcgagatg acccccgtgt tggattgtcc cgactttatt attcatgcct ctccagtcca
     1021 ccatatgatt cccaacattg gtttacgcgt caatttccaa caaagccaaa aagtgatggc
     1081 atattcatgt gacactgaac cttgtgacga agttgttcgc ttaagcgaag gtgcagacgt
     1141 gctcatccac gaagcgacgg gcgaatcgcc cggtcactcc tctgccacgc aagccggaga
     1201 aatcgcttcg cgggcagaag taaataaact gtatctcatt cactatccca ccggcagatt
     1261 caaaaaaggc gatattgccg ccgaagcatc tcagacctat cagggcgaga tccagatagc
     1321 aaaagacttt atggtgctag acttctaata aaaaatccct tcgtgatgag gggattttca
     1381 tttaccaacc ggtaatataa ggtgtccatt cttggttttc tgccaagtgt ttcccaaaaa
     1441 tataagaagc gtcggcaggt ggttctttgg ggatatggag caaatgcatg tgcgcttcat
     1501 ccactttgcg cccgcctttg cgatgattac aaggcgaaca agccgttacc aaattcgtcc
     1561 aaacttgttt accacccata tggcgcggaa tcacatgatc gatcgtcaag ttcgtgtcgc
     1621 gtttgccgca atactggcag gtataattat cgcgcctaaa gatctcgcgg cgcgttaatt
     1681 tgacgcgtgg gcgtggtcga tggatttgcg attccagccg aataaccgaa ggacgaggga
     1741 tgagctgtga tatggtgcgg atgtgtccgc gtccgtttgc gatcataccg gcttttcccg
     1801 caaggatcaa agtaactgcc cgtcgtgtcg aacagacgtg gatcggctca aaatttgcgt
     1861 tgagcaccag tacgggttcg ttcataaggt tccggtaact cgcgtgatta tacatctaga
     1921 atgttaaatc agtttaaaca cccattgaca tatcattgca ggagcaaaaa tgaatatcat
     1981 gatctctggc ggcacaggct tgcttggcac cgccctgaca aaatcccttc ttgcggatgg
     2041 tcataaagtt ttcatccttt cgcgtaccga caaaaaacgt tctgacgttc aaatcataaa
     2101 atgggatgcc cgtacccccg atgggtgggg gcatctcgtc aacgaaatgg atgtcgtcat
     2161 tcaccttgcc ggacgcagta cctccgcctg gccgtggtct gctgcaaata agaaatcctt
     2221 tgaggactct cgcatccttc ccggacttgc gcttgctcaa gccatccaag cagcgactcg
     2281 ccgcccgagc cttttcgtgc aagtctccgg catcaatcat tatggcttac atggtgacct
     2341 tgccgatgaa tccactccac ccggcgatga cttccttgct caactcaccg ttaaatggga
     2401 aggtgccact caatctgttg aaagattagg cgttcgcagg ttggttttgc gtacaccacc
     2461 tgtactttcc cgagataatg taataatgaa attgattgct ttgccagtgc aattattcgt
     2521 tggcggaccc attggttctg gcaaacaacc cttcccttgg attcatatca aagattgggt
     2581 aagtgctgtc cgctatttga tggcgaatga aagctcacgt ggcgtctata atatgattgc
     2641 gccttcgcag acctcattgg ctgatttcac caagatttta gccaatgtat tgcatcgccc
     2701 atattggttt cccgtcccag cctttttaat gcggaacgtt ttgggtgaaa tgagcgtctt
     2761 gatccttgaa ggaaggtttt cccagcctaa acggttgctt gagtctgggt acacgttcca
     2821 gtttccgggt ccgcgagagg cgttgaccga tttatttggt taagtcaaag gagataacaa
     2881 agtccatgaa gaagatgtct attgctttgc tgttaatgat tctgatatcc gcttgtgccc
     2941 ctgcacaaga ttcgcaggtg gtgttaccac agttattgcc aaccgctaca gcctatattg
     3001 atgcatcgtc atacccaaca gcagaagcaa atgtaagcgc tgctcaacaa accgcagcag
     3061 ggatcgatgt acgcatgaac cgtgcatgga tggaaggaaa gaatgtcaat gcagaagttt
     3121 gctttacctt gcccgatgcg tcagactggt cgatttgggc ggcgaattta gcttacagtg
     3181 gcattgtgct gcaagaatac ggaaccacat tgcagagcat tcaggaatca gcaaatggtc
     3241 agcctggtgt gcgttgtgat attttgacct ttgttgttgc tcccgatgca gacctgacca
     3301 atgccgccat caatatcgat gcaattgcag cccctccaca acccagcgaa tattgtgact
     3361 tgtatctgcc aaagattcaa caagctatgc tggagcgcgg aacgggcatc tctgtggact
     3421 gtgtggacgt taacggtgct ttgacaatgc aaatcctaaa caagcccgct gagatgtcac
     3481 aggaacaagc agaacaactt gtgtatagcg atgagttcta caccgtacgc ggaccgtgga
     3541 gcttcccttt caatttgtcg caataaaatt cctgatgtat aataccgagc atgtctggca
     3601 actt
//
LOCUS       DAOQFT010000236         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14724_length_3595_cov_6.803672, whole genome shotgun sequence.
ACCESSION   DAOQFT010000236 DAOQFT010000000
VERSION     DAOQFT010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14724_length_3595_cov_6.803672"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..785)
                     /locus_tag="PLR93_07045"
     CDS             complement(126..785)
                     /locus_tag="PLR93_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNH26758.1"
                     /translation="MWKTIYTWLDERIGLNDLYTTMLDRPEPKTTWWNTLGSASMFLF
                     ILQGLTGIFLTVYYTPSPDHAYDSINYIMEGVAFGWLIRGIHHWGATLMVVLVFIHML
                     RVFYTASFKYPRELTWLIGIGLFLTTLGMGFTGYLLPWNQKSYWATTVGTQIAGTVPF
                     LGDFILKALRGGPDLSALTLQRFFSAHIWIIPATLAALMGIHLFLVIKHGESHWPQKE
                     D"
     gene            complement(788..1285)
                     /locus_tag="PLR93_07050"
     CDS             complement(788..1285)
                     /locus_tag="PLR93_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNH26759.1"
                     /translation="MSDSHEVSRRDFIKVTTGIVGGLIGTMVGLPSIFYLIDPALQED
                     AKEAWIPIGKFEDMQVGVPYPFSFTQVQVNGWERTASSFGGYAIRKSDNPDELLILNS
                     RCTHLSCTVNWSAEAQAYICPCHDAKFGMEGEVLDGPPPRPLDIYEEHRITADGIIEI
                     FFKGA"
     gene            1617..1991
                     /locus_tag="PLR93_07055"
     CDS             1617..1991
                     /locus_tag="PLR93_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26760.1"
                     /translation="MGLKRDVSLSTGLRYRGPAGYLTYILHRIGGLGMGIFITLHILA
                     SFVGGRFGGALNSIYESWIFQIFIFFCVLFHAINGLRITILDLFPKLLEHQTQAIWIE
                     WAVFIPVYGISVFVILKTALGG"
     gene            1993..2433
                     /locus_tag="PLR93_07060"
     CDS             1993..2433
                     /locus_tag="PLR93_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26761.1"
                     /translation="MATVNKSRRLSIKARGLTNFESIMWLFTRLSALAMYALILFGLL
                     GALVMGARNNMNFADVMRWAFMPNVTHVQSTDLASTDAWATPLWKLTASALLLVATAH
                     GVHGLVVIADDYIAGETGRKIVRFFSIVVMVGWSLMGLWLIWTS"
     gene            2515..>3595
                     /locus_tag="PLR93_07065"
     CDS             2515..>3595
                     /locus_tag="PLR93_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH26762.1"
                     /translation="MANVHQFDVVVVGAGGAGLMAGLYASRNAKTAVISKLYPTRSHT
                     GAAQGGISAALGNYEEDKPEWHMYDTVKGSDYLGDQDAIEFMTTEAIDAVLELEHMGL
                     PFDRTPEGKISQRPFGGHTNNETGKPVRRAAHAADRTGHMILQTLYQQCLKNKVNFFD
                     EFQALDFIMVDGKAAGIVAIELATGELHTFHAKSIVFATGGFGRIFEVTSNAYAYTGD
                     GAAMLLRKGLPLEDMEFFQFHPTGIYKLGILITEGARGEGAVLINGKGERFMPKYAPT
                     VKDLASRDVVSRAIYTEIKEGRGIDGKNYVYLDIRPETVNKYAAEDGRTNPDGSPYKI
                     TGDTVLKKIPDIVDFCRVYLGVDPVT"
BASE COUNT          828 a          813 c         1015 g          939 t
ORIGIN      
        1 aggatgaaaa ttcccagcga catgatgagg tcgcggaaga tggtatcggg ccagaagcgt
       61 tcgcctttgt cgagttcgcg ttcgtagcgt gcgttgattt tctttttggt ttcgtcgttc
      121 atggcttaat cttccttttg gggccagtgc gattcgccgt gcttgatgac gaggaataaa
      181 tggatgccca tcaatgccgc tagagtggca gggatgatcc agatatgagc ggagaagaag
      241 cgctgtaatg tgagggcgct gaggtcaggt ccgccgcgca aggctttgag gatgaaatca
      301 ccgaggaagg ggacggttcc ggcaatttgt gtgccgacgg tggttgccca ataggatttt
      361 tgattccatg gcagcaaata acctgtgaag cccatgccaa gagtggtgag aaataagccg
      421 atgccgatga gccaggtcag ctcgcggggg tatttgaagg aagcggtgta gaacactcgc
      481 agcatgtgga tgaagactaa gacaaccatg agggttgcgc cccaatgatg aatgccgcgg
      541 atgagccaac caaacgctac cccttccatg atgtagttga tggagtcgta ggcgtgatct
      601 ggggaggggg tgtagtacac agtgagaaag atgccggtga gcccttgtaa aatgaaaagg
      661 aacatgctgg ctgagccgag cgtgttccac caggttgttt tgggttcagg gcggtcgagc
      721 atggtggtat acaggtcatt gagaccgata cgttcatcga gccaggtgta gatcgttttc
      781 cacatggtta tgctcccttg aagaagatct caatgatgcc atctgcagtg atgcgatgct
      841 cttcatagat atcgagcggg cgaggtggag gtccatctaa cacttcgcct tccatgccga
      901 acttggcgtc gtgacagggg cagatatatg cctgtgcttc agccgaccag tttacggtgc
      961 aggaaagatg cgtgcaacga ctgttgagga tgagcaactc atcggggtta tccgatttgc
     1021 ggatggcgta tccgccaaag gaactggcag ttcgctccca gccgttgacc tgtacttggg
     1081 tgaacgagaa ggggtagggt acgccaactt gcatatcttc gaatttaccg atgggaatcc
     1141 acgcttcttt tgcatcctcc tgcaatgcgg ggtcgatcag atagaaaatg gagggaagac
     1201 cgaccattgt gccgatcaaa cctccaacga tgcccgtcgt taccttgatg aaatcccgac
     1261 gggatacctc gtgtgaatca gacatgtgcg cctccaaggg gaatatattg cgcttgccat
     1321 tcgactagtg gataaaggtg gaacgatgcg gcgttaagag ctattggaat tggattataa
     1381 gggtctctgt tcccttcatt ataggacggc aggaatgaaa ttagcaggat ttttgtcacg
     1441 tttcgctatt tcggctgagc aggcagtttt attaaaatcg atagccgtgc ggtattagaa
     1501 gagtcttttc agtattaaaa ggatggaaat accccattgt ccatctttcc acttaaactt
     1561 ttaagagata gcgagtagaa tatgcgcttg taacaaaatt caccacggag gcaatcatgg
     1621 gcttgaaacg ggatgtcagc ctctccacag gtttgcgtta tcgcggacca gcggggtacc
     1681 tgacttatat cctgcaccgc attggtgggt tgggcatggg tattttcatt accttgcaca
     1741 tcctcgcttc atttgtcggc ggcaggtttg gcggggcatt gaatagtatt tatgagagct
     1801 ggattttcca gatcttcatt ttcttttgtg tgttgttcca tgccatcaac ggcttgcgca
     1861 tcaccatttt ggatcttttc ccaaaacttt tggaacatca aacgcaagcc atttggatcg
     1921 agtgggcggt cttcattcca gtgtatggca tttcggtttt cgtcattttg aagactgcgt
     1981 tgggagggta acatggctac tgtcaataaa tcgcgacgcc tctccatcaa agcccgtgga
     2041 ttaaccaatt ttgaatccat tatgtggctg ttcacacgcc tctctgcttt ggcgatgtat
     2101 gccttgatcc tctttgggtt gctcggcgct ctggtgatgg gcgctcgcaa caatatgaat
     2161 tttgccgatg tgatgcgctg ggcattcatg ccgaatgtga ctcatgtaca aagtaccgat
     2221 ctggcaagca ccgatgcctg ggcaaccccg ctttggaagt tgaccgccag cgccttgctc
     2281 ttggtagcta cggctcacgg cgtgcatggt ctggttgtga tcgcagatga ttacattgcc
     2341 ggtgagaccg ggcgcaagat cgtgcgtttc tttagcattg tagtgatggt tggatggagc
     2401 ctcatggggt tgtggctcat ctggacttcg taaaatggat gatggacagt agatgataga
     2461 ccatggtcta tcatccatcg tttatcgtcc gtaatttttc aggagcaata tttcatggcg
     2521 aatgttcatc aatttgatgt ggtggtggtg ggagcaggcg gcgccggttt aatggcgggt
     2581 ttgtatgcct cgcgcaatgc caagaccgct gtcatcagca aactgtatcc tacgcgttca
     2641 cacactggcg cggcgcaggg tggcatctct gcagcgttgg gcaattacga agaagataaa
     2701 cccgaatggc atatgtacga taccgtcaaa gggtccgatt atcttggcga ccaggacgcg
     2761 atcgaattca tgacgaccga agcgatcgat gcggtgctcg aactggagca catgggtctg
     2821 ccttttgacc gtacccctga aggcaagatc tctcagcgtc cgttcggtgg gcacaccaac
     2881 aacgaaactg gtaaacctgt gcgccgtgct gctcacgccg cagaccgcac cgggcatatg
     2941 atccttcaga ccttgtatca gcagtgcttg aagaacaagg tcaatttctt tgatgaattt
     3001 caggctctcg atttcatcat ggtagatggc aaggcagcgg gcattgttgc gattgaattg
     3061 gcaaccggcg aactgcacac cttccatgcc aaatccattg tgtttgcgac gggcggtttt
     3121 ggtcgtatct ttgaagtgac ttcgaatgct tacgcctata ccggcgatgg tgcagcgatg
     3181 ttactgcgca agggacttcc gctcgaggat atggaattct tccaattcca cccgaccggt
     3241 atttacaaac tcggtattct catcaccgaa ggggcgcgtg gtgaaggtgc ggtgctcatc
     3301 aacggcaagg gcgaacgctt tatgccgaag tatgcaccga ccgtgaaaga cctcgcttcg
     3361 cgtgatgtgg tttcccgcgc gatctatact gagatcaaag aaggtcgcgg tattgatggt
     3421 aagaattatg tgtatttgga tattcgccca gagacggtga ataagtatgc cgcggaagat
     3481 ggacgtacca acccagacgg ttctccctat aagatcactg gggatactgt tctcaagaaa
     3541 attcctgaca ttgtggattt ctgccgtgtc tatctcggcg tggaccctgt caccc
//
LOCUS       DAOQFT010000237         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14730_length_3594_cov_7.135632, whole genome shotgun sequence.
ACCESSION   DAOQFT010000237 DAOQFT010000000
VERSION     DAOQFT010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14730_length_3594_cov_7.135632"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PLR93_07070"
     CDS             <1..891
                     /locus_tag="PLR93_07070"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNH26763.1"
                     /translation="GYFGGRLAQHGQDVTFIARGAHLVAIRDAGLKVESVDGDFTVHP
                     VNVTDSPKTVGSVDLILLAIKAWQLEDAIEQMKPMVGESTVILPLLNGMEHMDSLFAQ
                     FGAHVIGGFCRISAFIGSPGVICHVGVKPYIAYGEWDNSKSERIQSIHDLFRSISGIV
                     AEIPIDIQTAMWEKFIFIAGTSGVGAYTRQTIGEYRANPETRTMLQHAMQETSAIAYA
                     KGVNLAADFVDFTMKRIDTIDPNITASTQKDIMAGKPSELNQQTGAVIRMGKAAGVPT
                     PTHEKIFAALLPLEQKARAQ"
     gene            986..1888
                     /locus_tag="PLR93_07075"
     CDS             986..1888
                     /locus_tag="PLR93_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNH26764.1"
                     /translation="MSKNIFSELSEAMANAAEKAGKYTVLVDARRRMPASGIAIDKET
                     ILTAGHVVEREEDIKILFADGKEVTARLIGRDPGTDLAVLKLDSAVATPAELAKTPAR
                     AGQFVLAVGRPSANGIESSFGTVNKISGPVRTGRGGMLEKYIKTDVVSYPGFSGGPLI
                     NGDGEVYGINTSGFGMGGEAITIPADLAWKIAETLTKHGKIKRGFIGVRSQTVNLPAD
                     AQAQLNREQETGLLLVGIEKDSPAEKGGLIIGDILVGVAGVAVEHHDELFTRLSGDVV
                     GKPTAMDVLRGGKLQSVSVVVGER"
     gene            1948..2553
                     /locus_tag="PLR93_07080"
     CDS             1948..2553
                     /locus_tag="PLR93_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26765.1"
                     /translation="MIRVTIVSPNHALRVGLREMLANQAGIRVVGEATRLSDVNERET
                     EVAVLASVSSVRSVDQKDFCILFLTDDIESVRPLLSSGMSAWGVLSSEANADELAAGI
                     AAVAEGLWVGAPGLVEGLMRLPKGGEGMGEESLPEPLTAREKEVLQHMAEGLANKQIA
                     LALRISEHTVKFHLSALYAKLGAASRTEAVKRGIELGLISL"
     gene            2626..>3594
                     /locus_tag="PLR93_07085"
     CDS             2626..>3594
                     /locus_tag="PLR93_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNH26766.1"
                     /translation="MPNEFHYKWEWDLASSPEALWPLASDTNRFNRDTGLPPMQLLGI
                     QNRVKQVKFKVPIVNIVWDEEPFEWTYPYRFGILRRYRTGPLQEMRVDCRLERRDAGT
                     HLIYEVWVKARNVLGDIAIPLGIGIYSAKRFEDAFKLYDRIASTSNKLPGTTTKQNLS
                     SSGHSRYRLLRERLIAQNVDQTTLDRLYEYFQHADDLSIQRMRPYALADGWNLPRRAV
                     LEAFLFATREGILDMYWDLLCPECRGVAEDHKRLGDLHAHSHCNTCQIDFNVNFDQNV
                     EVIFRPNPSIRQVDTSIEFCVGSPQRQPHIVFSLIVPPREQLPFNTM"
BASE COUNT          905 a          810 c          951 g          928 t
ORIGIN      
        1 ggatatttcg gcgggcggct ggctcagcat ggacaggacg tcacctttat tgcgcgagga
       61 gcgcacttag tagctattcg tgatgcagga ttaaaagtcg aatctgtaga cggggatttc
      121 acggttcacc cggtcaatgt aacggattct ccaaagacag tgggtagcgt tgatttaatt
      181 ttgcttgcca tcaaagcctg gcaattggag gacgctattg agcaaatgaa acccatggtt
      241 ggagaaagta cagtcatttt gcctttgctc aatggtatgg aacacatgga ttcgttgttt
      301 gctcagttcg gcgcacatgt aattggtggc ttttgtcgta tcagtgcttt cattggcagt
      361 cccggtgtga tttgccacgt aggcgtcaaa ccatatattg cctatggcga atgggataac
      421 tccaagagtg agcgcattca gtctattcat gatttgtttc gttcaatcag tggcattgtg
      481 gcagaaattc ctattgatat tcaaaccgca atgtgggaga aattcatctt tattgctggt
      541 acaagcgggg taggagcata cacgcgacag acaattggag aatatcgcgc caaccctgaa
      601 acacgcacta tgctgcaaca tgccatgcaa gaaacttcag ccattgctta tgcaaaaggt
      661 gtgaatctag cggctgactt tgtagatttc accatgaaac gtattgatac gattgaccca
      721 aacataacgg catccacgca gaaggatatc atggcgggca aaccttccga gcttaatcag
      781 caaactggtg cggtcattcg tatgggcaag gcagcgggtg tgcctacccc aacccatgaa
      841 aaaatttttg cagccttgtt gccgctcgaa caaaaggctc gtgcgcaata atacccacta
      901 gccgaatgtc tagtatcgcc ccagtcaatt caccgataca ctccaaacac atgctcgcta
      961 gaatgagcat aaaggagtaa cgaaaatgag caaaaatatt tttagtgaac tttctgaagc
     1021 catggcaaat gctgccgaga aggctggcaa atatacagtc ttggtcgatg cccgccgccg
     1081 catgcctgcc agtggaattg caattgataa agaaacgatt ctgactgcag gtcacgtagt
     1141 agaacgcgaa gaagatatca agattttatt cgcggatgga aaagaagtca ctgcacgcct
     1201 catcggtcgg gacccgggaa cggacctcgc cgtcttgaaa ttagactccg ctgtagccac
     1261 tcctgccgaa ctcgcaaaga cgccagcacg tgcggggcag tttgtcctcg ctgtggggcg
     1321 cccttccgcg aatggaattg aatccagctt tggcaccgtg aacaaaatta gtggacccgt
     1381 ccgcaccggg cgcggtggca tgttggagaa atacatcaag accgatgtgg tctcttaccc
     1441 cggtttctct ggcggaccac tcatcaacgg cgatggcgaa gtgtatggca tcaacacctc
     1501 cggctttggt atgggcggag aagcaatcac catccctgct gatttggcat ggaagattgc
     1561 agagaccctt accaaacatg gcaagattaa acgcggcttt atcggtgttc gcagtcaaac
     1621 cgtaaatctc cctgccgatg cgcaagcaca acttaaccgt gagcaagaaa caggtttgct
     1681 gttggttggc attgaaaaag acagtcccgc agaaaaaggc gggctgatca taggcgatat
     1741 tcttgtcggt gtcgctggcg ttgccgtgga gcaccacgac gaattattca cccgtttgag
     1801 cggcgatgta gttggcaagc ctactgccat ggatgttctt cgtggtggta agttgcaatc
     1861 ggttagtgtt gttgtaggtg aaagataaaa atataaagag cacagagcat aaaactctgt
     1921 gctctttata gcaagaaagg ctttattttg atccgtgtaa ccatcgtctc accgaatcat
     1981 gccttgcggg ttgggttgcg tgaaatgctt gccaaccaag cgggcattcg tgtggtgggg
     2041 gaggcgactc gtctttcaga tgtcaatgaa cgcgaaaccg aggtggctgt gcttgcctcg
     2101 gtttcttctg tacgtagtgt agatcaaaaa gatttttgca ttctttttct gacagatgat
     2161 attgaatccg tacggccttt attatcatcg gggatgagtg cgtggggggt cttatcttct
     2221 gaagccaatg cggatgaatt agcagcggga atcgctgcag tggcagaagg gctttgggtg
     2281 ggcgcacccg gcttggtcga gggattgatg cggttgccaa agggcggcga aggcatggga
     2341 gaagagtctt taccagagcc tctcacggca cgcgagaagg aagtcctcca gcatatggcg
     2401 gagggacttg ccaacaagca aattgcgctg gcgttgcgaa ttagcgaaca tacggttaag
     2461 tttcatcttt cggcgttgta tgccaaattg ggggcggcaa gccgcaccga agctgtgaag
     2521 cgcggcattg agttggggtt gatttcgttg taatttctta tttgtctgta atgctgttga
     2581 caggtttggc aggtaaaata tttagacttt ataggtctat atatcatgcc aaacgaattt
     2641 cactacaaat gggaatggga cttggcgtct tcgccggaag cattgtggcc gcttgcgtca
     2701 gatacaaatc gcttcaatcg tgataccggc ttgccgccca tgcagttgtt ggggattcag
     2761 aatcgggtca agcaggttaa gttcaaggtg cccattgtaa atattgtatg ggatgaagaa
     2821 cctttcgaat ggacgtaccc gtaccgcttt ggaattcttc gccgctatcg aaccggtccg
     2881 ctgcaagaga tgcgtgtcga ttgccgcttg gagcgccgag atgctggcac acatttgatc
     2941 tacgaagtgt gggtgaaagc ccgcaatgtc ttgggggata tcgctattcc gctgggaatc
     3001 gggatctata gtgcgaaacg atttgaagat gcattcaaat tgtatgaccg catcgcttca
     3061 acttctaaca agctgcctgg tactaccacc aaacagaatc tcagctcaag cggacattcc
     3121 cgctatcgtc tattgcgcga gcggctaatt gctcaaaatg tagatcaaac caccctcgac
     3181 cgtttgtatg aatattttca acatgctgat gacctatcca ttcagcgtat gagaccctat
     3241 gccttggcag atggttggaa tctgcctcgc cgtgctgtgt tagaggcatt tctttttgcc
     3301 actcgcgaag ggattttaga tatgtattgg gatttgctat gcccagagtg ccgaggcgtg
     3361 gcagaagacc ataaacgttt gggcgatttg catgcacatt cgcattgcaa tacttgtcag
     3421 atcgatttca acgtgaactt tgaccagaat gtagaagtga tcttccgtcc gaacccatct
     3481 attcggcaag tagatacatc gattgagttt tgtgtaggca gcccacaacg ccaacctcat
     3541 attgttttct cgttgatcgt cccgccgcgt gaacaattgc cttttaatac catg
//
LOCUS       DAOQFT010000238         3590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14752_length_3590_cov_6.767468, whole genome shotgun sequence.
ACCESSION   DAOQFT010000238 DAOQFT010000000
VERSION     DAOQFT010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14752_length_3590_cov_6.767468"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..793
                     /locus_tag="PLR93_07090"
     CDS             113..793
                     /locus_tag="PLR93_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26767.1"
                     /translation="MPELILVVDDEPKIVRIARDYLEKNGFRVITAADGHAALATARR
                     EKPDLIVLDLMLPLMDGREVCKTLRRESDVPIIMLTALAEEVDQVTGLEIGADDYITK
                     PFSPRALVARVRALLRRAKGEVKPPSIIRVGGLEIDPDRYSVTFNDEPVKLTPNEFKL
                     LQLLSARPGQTLTREQLLEDMHGIASSFDRSVDSHIKNLRKKLETASGENMIETVYGI
                     GYRFIEIK"
     gene            804..1865
                     /locus_tag="PLR93_07095"
     CDS             804..1865
                     /locus_tag="PLR93_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH26768.1"
                     /translation="MRRPHFPQRHPSNIERRRRRFFPFAFFFFGIFSLFFGGVAAILY
                     WAFTEYSGFRNIWLLVCAAPIALLILGVFTIFTMYMRFGKPLGDIFNAIDSVAEGNLS
                     VRVDESKSDMFSELIKRFNKMVEELERAEQQRRNLTADIAHELRTPLHIIQGNLEGVI
                     DGVYQPSPEHINNTLDETKLLARLVDDLQTLSLAETGQLPLHPTRFLLADLTADLITS
                     FSAQAKTQGIELTAHNADGEKTIEADYDRLNQVLSNLISNALRHTPADGKISIETESI
                     TSGERSVRITIQDSGSGIASEDLPFIFDRFWRADKSRTNRSNSGLGLAITKQLVLAHK
                     GDIRVESEIGKGTTFIIEI"
     gene            complement(1911..2597)
                     /locus_tag="PLR93_07100"
     CDS             complement(1911..2597)
                     /locus_tag="PLR93_07100"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNH26769.1"
                     /translation="MEQMPSNTPVMESNPGPAGWVQTWIKAVTKPNEQTFIDISESPD
                     AKIQTALIWAAIAGLISGVVIGMGFALQQLIQGGNISDKVVTIAVAICGFPLAMAVIN
                     PIALSFSTALFQWVAKLFGGVGTFEKLIYPISAITVPMTIVSSVFSFLSSIPLVGACL
                     SIFSIAISIYATVLNIMAVKAVNRFDWGQAIASVLIPAFVIGIFCGCIVFIGMMAFMP
                     VISNAFGSFN"
     gene            2735..>3590
                     /gene="uvrC"
                     /locus_tag="PLR93_07105"
     CDS             2735..>3590
                     /gene="uvrC"
                     /locus_tag="PLR93_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNH26770.1"
                     /translation="MEVSEKLQGILATLPTKPGCYLYRNAEGTIIYVGKAINLKNRVR
                     SYFHADSSHDNKTRRLVRDIADIEWIVVGSELEALILEMNLIKKHRPKYNIRLKDDKR
                     YPYIKVHWNEPFPKITVTRQMEEDGSRYFGPYTSAWAVYQTLEVLRKIFPYLTCDREI
                     TGNDPRACLYYDIKLCTGPCIGAVSKDGYRQMISDLMEFLSGHSEPIVERVQADMLKA
                     SEEMRFEKAAALRDQLKAMQSIIERQKIVFGSDYADSDVIAMAREDGEACVQIFFIRG
                     GKLIGREYF"
BASE COUNT         1003 a          886 c          798 g          903 t
ORIGIN      
        1 aagaaagagt aacgtgtgaa aaaaagaaga aagaggaaga aacgcctctt tcttctttcc
       61 ctttcttaag ccctcactat ccaccattcc tcattccctt tatactttcc ccatgcctga
      121 actaatcctc gttgttgatg atgaacccaa gatcgtgcga attgcgcgcg actatttgga
      181 aaaaaatggc tttcgcgtga ttacggctgc cgacggtcac gccgcgctgg caacggctcg
      241 ccgcgaaaag cctgacctga tcgttttgga cttgatgctc cctctcatgg acgggcgtga
      301 agtgtgcaaa actctgcgcc gtgaaagcga tgtgcccatc atcatgctca ccgctctcgc
      361 cgaagaagta gaccaagtca ccgggctgga gatcggcgcc gatgattaca tcaccaaacc
      421 cttttcgccg cgtgccttgg ttgcgagagt ccgcgccttg ttgcgacgtg caaaaggcga
      481 agtgaaacct cccagcatca tccgtgttgg tggcttggaa attgacccag atcgctactc
      541 ggtcacattc aacgacgagc ccgtcaaact tactcccaat gagttcaagc ttttacagtt
      601 gctctccgcc cgcccgggac agaccctgac ccgtgaacaa ttactcgaag atatgcacgg
      661 catcgcatcc agctttgacc gcagtgtaga ctcgcacatc aagaatctac gtaaaaagct
      721 tgaaaccgct tccggagaaa atatgatcga aaccgtatat ggaattgggt atcgtttcat
      781 tgaaataaaa taaaaccatg cacatgcgtc gtcctcactt tccccaaaga catccatcca
      841 atatcgagcg ccgtcgaagg cgtttttttc catttgcatt tttcttcttt ggtatctttt
      901 ccctgttctt tggcggtgta gcggcgattt tatattgggc attcacagaa tattctggct
      961 ttcgcaatat ttggctgttg gtttgtgcag cgccaattgc cttactgatc ctcggcgtat
     1021 ttaccatctt caccatgtat atgcgtttcg gcaaaccgct tggtgatatc ttcaatgcca
     1081 ttgattctgt tgcagagggg aatctctcag tacgtgttga tgagagcaag tctgacatgt
     1141 tcagtgagtt gatcaaacgc ttcaataaaa tggtcgaaga actggaacgc gctgaacagc
     1201 aacgccgcaa tctcactgcc gatattgcac atgaattgcg cacgcctttg cacattattc
     1261 aaggcaacct cgaaggcgtc attgatggtg tgtatcaacc cagcccggaa cacatcaaca
     1321 acacattgga tgagaccaaa ctgctggcac ggctggtgga tgatctacaa acactctcac
     1381 tcgccgaaac aggacaactt ccgcttcacc ctacccgctt cctgcttgcc gatctgacag
     1441 cggatctcat caccagtttt tcagcacaag cgaaaactca aggcatcgaa ttgactgctc
     1501 ataacgcaga tggtgagaaa acgattgaag ccgattatga caggcttaat caggtattat
     1561 ccaatctcat ttccaatgca ttaagacaca cgcctgcaga tggaaaaatt tcaatcgaaa
     1621 cagaatccat aaccagcgga gaaagaagcg tgcgcatcac catccaagat tcaggttctg
     1681 ggattgcatc cgaagacctg ccctttatct tcgatcgctt ttggcgggca gataaatcac
     1741 gaaccaatcg ctcgaatagc ggcttgggtc ttgcgattac aaaacagctt gtgctggcac
     1801 acaaaggtga cattcgtgtg gaaagtgaaa ttggaaaagg cacgacattt atcatcgaga
     1861 tataaaaaag tcctcccaaa tgggaggact tttttatcgt ttgtgtatct ttaattgaag
     1921 cttccgaaag cattcgaaat gacaggcata aacgccatca taccgatgaa cacgatgcag
     1981 ccacagaaga tgccgattac aaaagccgga atcaataccg aagcaattgc ctgcccccag
     2041 tcaaaacgat ttactgcttt aaccgccata atattcaaga cagtcgcata aatacttata
     2101 gcaatactaa aaatacttaa acacgcccct acaagaggaa tactacttaa gaagctaaac
     2161 acgcttgaaa caatcgtcat cggcacggta atggctgaaa ttggataaat aagtttttcg
     2221 aacgtgccta ccccgccaaa gagttttgca acccactgga acaatgctgt ggaaaaagat
     2281 agggcaattg ggttaataac tgccatggca agcggaaagc cacaaattgc aacggcaata
     2341 gtaactactt tatcagaaat attgccacct tgaatcagtt gttgtagagc aaaacccatc
     2401 cctatcacaa ccccgctaat aagccctgcg atggctgccc aaataagcgc tgtttgaatt
     2461 tttgcatctg ggctttcaga gatatcaata aacgtttgtt catttggttt tgtaactgct
     2521 ttgatccatg tttgaaccca gcctgcgggt ccggggttgg attccatgac aggtgtgttt
     2581 gaaggcattt gttccatttg aattctcctt tttctttcca aagtgatgac gtatccattt
     2641 taagcgatga tattccgctt gaccgtactg tatttcaacg gcaattttca gaacaaatat
     2701 cctaatttcg ataacaagcg gtagaattgc cgcaatggaa gtttcagaga agctacaagg
     2761 cattcttgcc accctgccca ccaaaccggg ctgttatcta tatcgcaacg ccgaagggac
     2821 aatcatttac gtcggcaagg cgatcaattt gaagaaccgc gtacggagct atttccatgc
     2881 cgattctagt cacgataaca agacccgccg cctggtacgc gacattgcag atattgaatg
     2941 gatcgtggtc ggctcagagc ttgaggcact catccttgag atgaacctca ttaagaagca
     3001 tcgtccgaag tacaacatcc ggctcaaaga cgataaacgc tatccataca tcaaagtcca
     3061 ttggaatgaa ccttttccaa aaatcacggt cacccgccaa atggaagaag atggctcgcg
     3121 ttattttggt ccatacacct cggcatgggc ggtgtatcag acgctggaag tgctgcgaaa
     3181 aatattcccc tacttgacct gtgaccgcga gatcacaggc aatgatccac gggcatgttt
     3241 gtattacgac attaagttgt gtacaggtcc gtgcattggg gcggtttcaa aagatggcta
     3301 ccgccaaatg atctcagact tgatggaatt cctcagcgga cacagcgaac ccatcgtgga
     3361 acgcgtccaa gcggacatgc ttaaggcatc tgaagagatg cgttttgaaa aagccgctgc
     3421 cttacgagac cagttgaagg cgatgcaatc catcattgaa cggcaaaaaa ttgtcttcgg
     3481 gtcagattat gcagactcag atgtgattgc catggcgcgt gaagatggcg aagcttgcgt
     3541 gcagattttc ttcatccgtg gcggcaaatt gatcgggcgc gagtacttca
//
LOCUS       DAOQFT010000239         3574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_14851_length_3574_cov_6.937482, whole genome shotgun sequence.
ACCESSION   DAOQFT010000239 DAOQFT010000000
VERSION     DAOQFT010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14851_length_3574_cov_6.937482"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLR93_07110"
     CDS             complement(<1..614)
                     /locus_tag="PLR93_07110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNH26771.1"
                     /translation="MDIQIDNLVRAKRRTIALIVERDGSLTVRAPRRATLNDIYGFIQ
                     EKTDWILRSREKLKAIKPIPKKAYVDGERFLFLGQEYELRLVPPQRTALKLDGGFTLS
                     TSAQERGELAFTKWYKTQALTIFTERVNHYANLHGLIPKEVKVNSAKTRWGSCTSAGT
                     LNFTWRLVMAPLEVIDYVVLHELAHLKVKNHSPRFWKLVESLCP"
     gene            complement(595..1914)
                     /locus_tag="PLR93_07115"
     CDS             complement(595..1914)
                     /locus_tag="PLR93_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH26772.1"
                     /translation="MNRTKNARVCIIGAGPSGITAGKNLIQAGVTNFVIYEKNDQVGG
                     NWVFNDKTKHSSVYETTHLISSRRYSEYFDYPMPADYPDYPSHTQMLAYFQGYAKHFG
                     VMPYIQFNTEVHNAEELPDKTWRITLGNSKVEIFDFLLIANGHHWNPRYPKYEGEFTG
                     EFVHSHEYKRAAPFAGKRVLVIGAGNSGCDISVETARVSAFTAMSMRRGQYIVPKFVF
                     GQPPDVVNGRFYWLPAWIRLPFFYLTLKLAVGKYEDYGLPKPKHGILNAHVTNNSEIL
                     YAIRHGKVHVRPDIKRFNGKQVEFVDGRVEEYDSIIAATGFKISLPFFDPNLLSIEEG
                     VPPLYKNVFHPDHPSLFFIGLVQPLGSIWPLSDLHAKLAANYIIGNYHLPKDIRQRIA
                     RHIEYMKKHYVNSPRHSVEVEFHPHIVELKREIPKSAPEWKHGYSDR"
     gene            complement(2014..2991)
                     /locus_tag="PLR93_07120"
     CDS             complement(2014..2991)
                     /locus_tag="PLR93_07120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNH26773.1"
                     /translation="MRPKVFVSRKIRDAGLNLVLEFCDAEIWDEEMPPTRDVLLEKVR
                     GVDGYLSMLTDRVDAELLDTAGPQLKVVSNHAVGFDNVVVSDATARGIPVGNTPGILT
                     DATADIAFALLLAAARRIVESEKYIRDGKWKTWHPSMLLGMDLAGRTLGLVGFGRIGR
                     AVAKRAVGFGMRIAYYSPNSEPEFGAIKMNSLDALLQESDFVSVHTPLNESTRHLVNA
                     DFLSKMKPNAVFVNTSRGPVVDQVALYDALKAKKIFAAGLDVTDPEPLPLDSPLLTLD
                     NCVIVPHIGSASERTRDAMARLAAENLIAGLKGERLPHSINPEVYENRR"
     gene            3078..>3574
                     /locus_tag="PLR93_07125"
     CDS             3078..>3574
                     /locus_tag="PLR93_07125"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HNH26774.1"
                     /translation="MQILNACIDAVEPGNAIRRFVQREKNLLFIDNKKYDLNEFGNVT
                     VLGLGKATEAMSLALLDVLGSYPSRGLLIPKQAFASPASEFTVSPGGHPVPSEASVRA
                     GNAAFNLASSLTERDLLICLISGGGSALMASPRHGLQLADLQTLTKLLLACGARVDEI
                     NTLRR"
BASE COUNT          860 a          915 c          884 g          915 t
ORIGIN      
        1 tcagggcaga gcgattctac caacttccaa aatcgcgggg aatgattttt gaccttcaaa
       61 tgcgccaact catggagcac gacataatcg atcacctcga gtggcgccat gaccaagcgc
      121 caggtgaagt tgagcgtgcc ggcagatgtg caagaacccc aacgtgtctt tgctgagttg
      181 actttgacct ctttcgggat aagtccgtgt aggttggcgt aatggttcac acgttccgtg
      241 aagatagtca atgcctgggt tttgtaccat ttggtgaagg ctagttcacc gcgttcttgt
      301 gcagaagtgg acagggtgaa tccaccgtcg agtttgaggg cagtccgctg aggtggaacg
      361 agccggagtt cgtactcctg cccaagaaag aggaaacgtt ctccatccac atatgctttt
      421 ttcgggatgg gctttatcgc ttttagtttt tcgcgcgaac gtaggatcca atcggttttt
      481 tcttggatga aaccatagat atcattcagt gtagcccgcc tcggcgcacg gaccgtaagg
      541 ctgccatctc gttcgacgat gagggcaatg gtgcggcgtt tggcgcgcac aaggttatcg
      601 atctgaatat ccatgcttcc attccggcgc agacttggga atctcgcgct ttagctccac
      661 gatgtgcgga tgaaactcta cttccacgct gtggcgcggc gaattgacgt aatgtttttt
      721 catatattca atatggcgcg caatgcgttg acgtatatct ttgggtaggt ggtaattgcc
      781 gataatataa ttagcggcaa gtttggcgtg caggtctgag agtggccaga tcgagccgag
      841 cggttgcacg agaccgataa agaatagcga tgggtggtcg ggatggaaaa cattcttata
      901 cagcggagga acaccttctt cgatggagag caggttgggg tcaaagaaag gaagcgagat
      961 cttaaatccg gtagcggcaa tgatcgagtc atattcttcc acgcgcccat ccacaaactc
     1021 cacttgtttg ccgttgaagc gtttgatatc tgggcggacg tgaactttcc cgtgacggat
     1081 ggcgtagaga atttctgagt tgttggtcac gtgcgcgttc aagatgccgt gtttgggttt
     1141 gggcaagcca taatcttcgt atttaccgac agcaagtttg agggttagat aaaagaaggg
     1201 caggcgaatc cacgcgggca gccagtaaaa acgaccattg accacatcag gtggctgacc
     1261 gaaaacgaac ttaggcacga tgtattgccc gcgtcgcatg gacatggcgg tgaacgccga
     1321 aacacgtgcg gtttctaccg aaatgtcaca gcccgagttg cccgccccaa tgacgagtac
     1381 acgcttccct gcaaatggtg ctgcgcgttt gtattcatgc gaatggacga attctcccgt
     1441 gaactcacct tcatactttg gataacgtgg attccaatga tgtccattcg cgatgagtag
     1501 aaagtcaaag atttctactt tgctattccc aagtgtgatg cgccaagttt tatctggtaa
     1561 ttcttcagcg ttgtggactt cggtgttgaa ttgaatataa ggcatcacac caaagtgttt
     1621 ggcgtacccc tgaaaatatg ccagcatctg cgtgtgcgac gggtaatcgg gatagtctgc
     1681 gggcatgggg taatcaaaat attccgaata acgccgcgag ctgatgaggt gcgtcgtttc
     1741 gtacacagac gagtgtttgg tcttgtcatt gaagacccaa ttcccgccga cttggtcgtt
     1801 cttttcatag atgacgaaat tggtgacacc tgcctgaatg agatttttcc cagccgtaat
     1861 gccagaggga cctgctccga taatgcaaac tcgtgcgttc tttgtgcgat tcatgtagcc
     1921 tcagattaaa tgaagtgaga gagttttacc ttgtttagac gatggatgat agaccattga
     1981 ccatggaact ttctatcgtc cattgtccgt ggtctatcgt ctattttcgt atacttcagg
     2041 attaatagag tgtggcaaac gttcgccttt caacccggcg atgagatttt ctgcggcgag
     2101 ccttgccatc gcatcgcgcg tccgctcgct ggcgctgcca atgtgcggaa cgatcacaca
     2161 gttatccaag gttagtaaag gcgaatccaa cggcaaaggt tcggggtcgg tcacatccaa
     2221 acctgctgcg aaaattttct ttgccttcaa tgcatcatac aaagcgactt ggtctaccac
     2281 tggtccgcgt gaggtgttga cgaagaccgc attcggcttc atctttgaga gaaagtccgc
     2341 gtttaccaaa tggcgcgtcg actcgttcaa gggtgtatgt accgaaacaa aatcagattc
     2401 ttgtaacagc gcatcgaggc tgttcatttt gatcgcgcca aattcaggtt ctgagtttgg
     2461 gctgtaatat gcaatgcgca ttccaaaacc aaccgcacgc tttgccaccg cccgtccaat
     2521 gcgaccaaac cccaccaacc ccaaagttct tcccgcaagg tccatgccca acaacatgga
     2581 tggatgccat gtcttccact tcccatcgcg gatatatttt tcagactcta caattcgtcg
     2641 tgctgcggcg agcaacaaag caaatgcaat atcagcagtg gcatcggtca aaatacccgg
     2701 cgtgtttcca accggaattc ctctcgccgt tgcatctgat acaacgacat tgtcaaaccc
     2761 caccgcgtga ttgctgacca ctttcaactg cggtccagcc gtatccaata attctgcatc
     2821 cacgcgatcg gtgagcatgc tcaagtaacc atctacaccg cgcacttttt caagtaacac
     2881 atcgcgcgtg ggtggcatct cttcatccca tatctcggca tcgcaaaact caagcaccaa
     2941 attcaaacca gcatcacgga tcttgcgcga aacaaatacc ttgggtcgca ttgcatctcc
     3001 ttctgcgtta tcatactcga aacatgcgca atttttccac tgtcacgctg caaactcatc
     3061 cccgcagaaa cgatatcatg cagattctaa atgcctgcat cgatgccgtc gaaccaggca
     3121 atgccattcg acgttttgtc cagcgcgaaa agaatttatt attcattgat aataaaaaat
     3181 atgatttgaa tgagtttggc aatgtgaccg tacttggctt gggcaaagcg acagaagcca
     3241 tgtcacttgc cttgttggat gtactcggct cttatcccag ccgcggcttg ctcattccaa
     3301 aacaagcctt tgcttcccct gccagcgaat tcaccgtctc gcctggcgga catcccgtcc
     3361 caagcgaagc gagtgtccgc gccggaaacg ctgccttcaa cctcgcctct tccctcaccg
     3421 aacgtgactt gctcatttgc ctcatctccg gcggtggctc agcgttaatg gcttccccgc
     3481 gacacggact tcaactcgcc gatcttcaaa cgctgacgaa attattgctc gcttgtggcg
     3541 cccgcgtgga cgaaatcaac accctgcgcc gtca
//
LOCUS       DAOQFT010000240         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15029_length_3546_cov_5.774563, whole genome shotgun sequence.
ACCESSION   DAOQFT010000240 DAOQFT010000000
VERSION     DAOQFT010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15029_length_3546_cov_5.774563"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(519..956)
                     /locus_tag="PLR93_07130"
     CDS             complement(519..956)
                     /locus_tag="PLR93_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH26775.1"
                     /translation="MYKKILVPLDGSTVAEGVLPHAKSLAYSEGAELILLTVAANPAM
                     DYLFSDPGIAETAVQEQVEKSKAYISTIENDLHSAGFKVSTLMRVGSVAEVILSVAEE
                     LQVDAIAMSTHGRTGAARWLLGSVAERVVHNSNVPVLLIRVKE"
     gene            complement(974..1705)
                     /locus_tag="PLR93_07135"
     CDS             complement(974..1705)
                     /locus_tag="PLR93_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNH26776.1"
                     /translation="MRILVVSDIHANFVAFEAVLKDAGSFDTVWCLGDVVGYGPQPNE
                     CVEQLRELHPVCLAGNHDLAVTGQVALWDFTKDAQEVIFWTRHWLTQENMEWLTTLSS
                     TPQVEHGITIVHASLRDPVWEYITNASLVKDNIQFAQTPICLHGHTHLATLFRKKWDE
                     YRILEEYPRLNTPIKLIPDQMFINPGSVGQPRDEDPRAAYAIINTDEMTYTQKRVLYD
                     VTTVQALMKQAKFSNRLIRRLRFGQ"
     gene            1788..3134
                     /locus_tag="PLR93_07140"
     CDS             1788..3134
                     /locus_tag="PLR93_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH26777.1"
                     /translation="MPSLFNRLFHKEKSTSHKIVAIKVGVVVNQRYQLESELGRGGMG
                     IIYKAHDLTKNREVAFKVINPETANSLSLNQFAREADILSSFDHPHIVSVHETGFVNE
                     DPSLPFLVMELLHGRPLSEIGVLTYSRIVSIAKQICEALTYIHSQGFVYRDIKPNNIL
                     LEKRGFDYFVKLIDFGLARPLGDAYLPNESSLAGTVFYLAPELIEGQVADVRSDLYAL
                     GILLYEMIVGRVPFSDVDEATIQLQHQQQKASPPSQSRLGVPMELDTLVLRLLEKNRQ
                     DRPASANEVCEILNSIRFDKKTQGNLPSNISDRSDAQPIIQLLAEHSLVTLISNDVPL
                     ALAAVSHLSEQFPDGVWVVNLEAVSEPVAVLPMVCSMFGVKEIQNRPLAVMSIEFLRE
                     KNLLLLLTHCGHVSGACAQLASAITVSCPDVQIMAVSDKPLNLPNEKVYSTQSKIL"
     gene            complement(3107..>3546)
                     /locus_tag="PLR93_07145"
     CDS             complement(3107..>3546)
                     /locus_tag="PLR93_07145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH26778.1"
                     /translation="HFLNYFRLWDNRMIFGGRAAFFPENENTIARSGEILRNEMIKVY
                     PQLKDANVDYVWGGTLDFAFDQMTHVGEEDGIFYSLGYAGHGVAMATYLGATVAEAML
                     KGNIKEHPFAKFDFPSAPMGLYNGNPWFLPFAGMYYKILDWVE"
BASE COUNT          921 a          868 c          794 g          963 t
ORIGIN      
        1 cggcagcaga tgcaaaaata atacggggta gactttgcgc ctcttcttgg tcagcagagg
       61 ctattacaga ctgttgtgac atattgcaat tctcctttgg gaaaacagtt gagtatgttc
      121 cgcgaatcgc ggttgtaaca ggctgggtga tcactaggca gtataagtca tcggcgctcc
      181 tttactccga gaacatccat taccccatgg acgagcagaa gaagcgttta tcaaaccgta
      241 tattggggag aagattcctt cgggcatttt tacctcgaac aaaaaccttg atccacctaa
      301 ccactaaagg gcttacatat ttgaggaaat aacactaagt ttgccgaatt cgttgtgtcg
      361 atttcaacgt caaatgtcat ggattttttt gatgattgtc atatggcaac ataaaaattg
      421 aatttcagac aaaaaatgtc cgaaagaata acggctcttt ccccgctctt gccgggcatt
      481 acactcaaaa aattagtttc cccgttagct caccccacct actctttgac acgaatcaat
      541 agaactggca cgttcgaatt atgcacaacc cgttctgcca cactgcccag aagccaacgc
      601 gccgcacccg tccgcccatg cgtggacatg gcaatcgcat ccacttgtaa ttcttctgcc
      661 acgctaagaa tcacctccgc taccgagccg acccgcatca aggttgagac cttgaacccg
      721 gcagaatgaa ggtcgttctc aatggttgaa atgtatgctt tgcttttctc aacctgctct
      781 tgaaccgcgg tttctgcaat gccagggtca gaaaagagat agtccatggc tgggttggcg
      841 gcgacggtca acagaatcag ctctgcgcct tctgaatatg ccagtgactt tgcatgggga
      901 agcactccct ctgcaacagt cgagccatcg agtggaacca gaatcttttt gtacataact
      961 tcctcctatc ggtttattgc ccgaaacgta gacgtcgtat gagccgattc gaaaactttg
     1021 cctgtttcat cagcgcttga accgttgtca catcataaag aacccgcttt tgagtgtaag
     1081 tcatctcatc tgtattgata atcgcataag cggcacgcgg atcttcatca cgtggttgac
     1141 ctacgcttcc ggggttgata aacatttggt cgggtatcaa tttgatgggt gtgttgaggc
     1201 gcggatattc ctccagaatg cgatattcat cccatttctt ccgaaagaga gtagcaagat
     1261 gagtatgtcc atgtaaacag ataggggttt gagcgaattg gatattgtct ttgaccaagc
     1321 tggcattggt gatgtattcc cagaccgggt cgcgcaagct ggcgtggacg atggtaatgc
     1381 cgtgctcaac ttgaggcgta gatgacaagg ttgtcagcca ttccatattt tcttgtgtga
     1441 gccagtgacg cgtccaaaaa atgacctctt gtgcatcttt agtgaaatcc cataacgcga
     1501 cttgacctgt tacggcaagg tcatgattgc ccgccagaca aactgggtgt aattctcgca
     1561 gttgttctac acattcgttc ggttgcggac catatcccac cacatcgccc aagcaccaga
     1621 ctgtatcaaa actaccggca tctttcaaca ccgcttcaaa cgcgacgaag ttcgcatgga
     1681 tatctgatac aacaagaatg cgcatggata cctaaaaata ttatagcgta cagaaagaat
     1741 gacgaaagta acttttttac agatgacagc aacgctataa tttgaacatg ccctctcttt
     1801 ttaaccgtct ctttcacaag gagaaatcca cttcgcacaa gatagttgct attaaggttg
     1861 gagttgttgt caaccaacgc taccaattgg agagtgagct tgggcgtggt ggtatgggca
     1921 ttatttataa ggctcatgat ttaactaaga accgcgaagt tgccttcaaa gtcatcaacc
     1981 ccgaaactgc aaattcactt tcgctgaacc aatttgcgcg tgaagcggat attctttcta
     2041 gcttcgatca tccccatatt gtttcagtgc atgagacggg gtttgtcaat gaagacccat
     2101 cactgccttt tcttgtgatg gaactcttgc acggaagacc cctttctgaa attggcgtac
     2161 tgacctattc tcgcattgtc tcaatcgcaa aacagatatg tgaagcgttg acatatattc
     2221 atagtcaggg ctttgtgtat cgtgacatca aaccgaataa tatcctgctc gaaaaacgtg
     2281 gatttgatta tttcgtaaag ctgatcgatt ttgggctagc ccgaccgcta ggggatgcat
     2341 atctgccgaa cgaaagcagc cttgccggaa ccgtgtttta cctcgccccc gaattgattg
     2401 aaggtcaggt cgctgatgtg cggtctgact tatacgcgct gggtatcttg ctgtatgaaa
     2461 tgatcgtcgg gcgtgtgccg ttttcggatg tggacgaagc cacaattcaa ctccaacatc
     2521 agcagcagaa agcatctcca cccagccagt ctcgcttagg cgtgccgatg gaattagata
     2581 cgcttgtttt gcgtctgctt gagaaaaatc ggcaagatcg cccggcatcc gcaaatgaag
     2641 tatgtgagat cttgaattcg attcgctttg ataaaaagac tcaagggaat ttgccatcca
     2701 atatttctga ccgttcagat gctcaaccca ttatccaact gcttgcggaa cattcacttg
     2761 tgactctcat tagcaatgac gtgccgcttg cgttagctgc tgtatcacat ctttcggaac
     2821 aatttccaga tggtgtttgg gttgttaact tggaagcagt aagtgaacct gtcgctgttc
     2881 ttccaatggt ctgttcaatg tttggcgtaa aagagattca aaatcgtccg cttgcggtca
     2941 tgtcgatcga atttctgcgc gagaaaaacc ttcttctcct tctcacgcat tgcggacatg
     3001 tctctggggc atgcgctcag ctggcatctg ccatcacagt ttcttgcccc gatgttcaaa
     3061 tcatggcagt tagtgacaag ccgttgaatt tacccaacga aaaagtttat tccacccaat
     3121 ccaaaatctt gtaatacatt ccggcaaacg gcaggaacca cgggttgccg ttatacaatc
     3181 ccattggcgc agaggggaaa tcaaacttcg caaacgggtg ctctttgata tttcccttta
     3241 gcatggcttc ggcaaccgta gcgcccaaat aggtcgccat tgccacacca tgcccggcgt
     3301 agcctagcga gtaaaagatc ccatcttctt cgcctacatg cgtcatttgg tcgaatgcaa
     3361 aatcgagcgt gccgccccac acatagtcca cgtttgcatc cttcaactgc gggtagactt
     3421 ttatcatttc attgcgcaat atctcgccgc ttcgcgcaat agtgttttca ttttcaggga
     3481 agaacgccgc ccgtccgcca aagatcatgc ggttatccca cagccgaaaa taattcaaga
     3541 aatgtt
//
LOCUS       DAOQFT010000241         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15069_length_3540_cov_6.238451, whole genome shotgun sequence.
ACCESSION   DAOQFT010000241 DAOQFT010000000
VERSION     DAOQFT010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15069_length_3540_cov_6.238451"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLR93_07150"
     CDS             <1..725
                     /locus_tag="PLR93_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNH26779.1"
                     /translation="LATVFVASAFPAALTSFIAAGTVVGVVLIGVAFTFLMSWILSRT
                     VLKGEASAFTLELPPYRKPNFGRILYTSLIDRTIFVLWRAMQTAAPAGAIIWLLANIP
                     FEGGNLASAIANWLNPFGLLLGLDGVILLAYIIAIPANEIVVPTMMMMYMGAGMMIDG
                     PSSNAAIFDLLVHGNGWTMLTAINLMLFALLHNPCATTIMTIYKETKSWKWATVSVVI
                     TLGTAFLVTFLTASFARMMGWA"
     gene            845..1528
                     /locus_tag="PLR93_07155"
     CDS             845..1528
                     /locus_tag="PLR93_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26780.1"
                     /translation="MKRHFWAGILILAIGFSLIGCGPATVGSNSFAGTWTTNLGAINF
                     MQNGNEITGNIEGYGGNWNETFTGSLNENGEAVFQTEVLGDFTLVLNGENIFKSTSTE
                     LSFCGIRGANQELPAGCGFSGKWIVPSKHVFLPGSYMILKQTGENVSGEIFDGNGNPY
                     ESFNGTMEWGKGWRANGTYKERGDISLWINASETGFEFVNPKGNIQQLCAVREGVASA
                     YLGSYSCEP"
     gene            1610..1900
                     /locus_tag="PLR93_07160"
     CDS             1610..1900
                     /locus_tag="PLR93_07160"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH26781.1"
                     /translation="MKLPELKAIKLAELFSSLSDASRIRIIASLLDGEMSVRVLAEGL
                     GMTESAVSHQLRGLRQMHLVRARKSGRQVFYSIEDDHVARLFTLGLDHVQHG"
     gene            1904..2842
                     /locus_tag="PLR93_07165"
     CDS             1904..2842
                     /locus_tag="PLR93_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNH26782.1"
                     /translation="MHTHSHTHIGDLTQQTTKRLTLSLVLTAAFVIVEIVAGVFGNSL
                     ALLTDAAHNFTDVIALGLSWYALRIAMKPAHAGKTFGYHRVGILVALINSTTLILIAF
                     GIFFEAYHRFLTPPEVDSTILIGVGAVAFIINLVTALMVKEGSEHDLNMRSAFLHLMG
                     DVMSTLGAVIAGIIIAFTKWNWLDPLVSVLIGAFILYNAWGILKQTIHILLESTPENI
                     NVDSMVLDLQNINGVLGVHDLHVWSISENLRMLSAHVVIGDTTIGEGVSIQQNINELL
                     AHSYNIHHATLQMECEGCTSKDLFCQITEAHQHAHH"
     gene            2860..>3540
                     /locus_tag="PLR93_07170"
     CDS             2860..>3540
                     /locus_tag="PLR93_07170"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNH26783.1"
                     /translation="MSQAAKAQQLLSLHNNGKLLILPNVWNPIGARMLQAKGCSAVAT
                     ASAAIAESLGYSDGEQLKLDTMLDMVARIVRSVDLPVTADFEAGYSDSIEGLQENISR
                     LLDTGAVGLNFEDSFDDSAHLRPIPEQVERIKAVREAANRKGIHLVINARADSFFAEG
                     FSSDEERLDDVITRLTAYTAAGADSVYPVGRGDKETLTVLRKRLSAPINVLATAKTAS
                     LKQLQELGI"
BASE COUNT          876 a          937 c          796 g          931 t
ORIGIN      
        1 tgcttgccac tgttttcgta gcttctgcct tccccgcagc cttgacctcc ttcattgccg
       61 ccgggacagt cgtcggcgtg gtcttgatcg gtgtagcctt cacattcctc atgtcgtgga
      121 ttttgtcgcg taccgtcctc aagggtgaag cctcggcatt cacgctcgaa ctgccgccct
      181 accgcaaacc aaactttggg cgcatccttt atacatccct aattgaccgc accatcttcg
      241 tcctctggcg agccatgcag acggcggcac ctgccggcgc catcatctgg ttgctggcga
      301 atatcccatt cgaaggtggc aatcttgcca gcgcgatagc caactggctt aatcccttcg
      361 gtttactact cggtctcgac ggtgtgattc tgttggcata catcatcgcc atccctgcca
      421 atgaaattgt cgtgcccacc atgatgatga tgtacatggg cgctggcatg atgatcgatg
      481 gaccgtcttc caatgccgcc atttttgacc tgctcgttca cggcaacggc tggaccatgc
      541 tcaccgcaat caacctaatg ctatttgcac tcttacacaa cccatgcgcc acaactatta
      601 tgaccattta caaagaaacc aagtcttgga aatgggcaac cgtcagtgtg gttattactt
      661 taggaactgc cttcctcgtc acattcctaa cagcaagttt tgccagaatg atgggttggg
      721 cataaaactc taccagcaaa gagacagtca ctcttaaggg tgactgtctc ttcttttaat
      781 cccctctttg tcggattgtg cctcatggga agagacgtta aagtaacccc ataggagaca
      841 aaatatgaaa cgacattttt gggctgggat actcattctt gccattggct tttcccttat
      901 aggctgcgga cctgctacag taggttccaa ttcctttgca ggaacatgga ccaccaatct
      961 tggtgcgatc aattttatgc aaaatggcaa tgagatcaca ggcaatatag aagggtatgg
     1021 cggcaattgg aatgagacct ttactggttc actcaatgaa aacggcgaag ccgtctttca
     1081 aaccgaagta ttaggtgatt tcacccttgt tctcaatgga gagaacatct tcaaatccac
     1141 ctcgaccgaa ttatcatttt gcggaattcg cggtgctaac caagaacttc ctgccggctg
     1201 tggctttagt ggaaagtgga ttgtcccttc aaaacacgtt tttctgcccg gaagctatat
     1261 gatcttgaag cagaccggtg aaaatgtgag tggtgaaatc tttgacggca atggcaaccc
     1321 ctacgaaagt ttcaatggca caatggaatg gggtaaaggc tggcgcgcca atggcacata
     1381 caaggaacgc ggcgatattt cgctatggat aaatgcctca gaaaccggct tcgaatttgt
     1441 aaacccgaaa ggtaacattc aacaactttg tgcagtgcgt gaaggcgtag caagtgcata
     1501 tctaggcagt tattcctgcg aaccgtaatt actcagaccc tgccaaacac catattgaca
     1561 gggtcttttt tacagagtaa tatatgaata gttgttcata tattcaatta tgaaattacc
     1621 cgaactcaaa gccatcaaac ttgccgaact attcagttcc ctaagcgatg caagtcgcat
     1681 tcgcatcatc gcatccctct tggatggaga aatgagcgtc cgcgtgcttg ctgaaggact
     1741 tggcatgacc gaatccgctg tatcgcatca gctccgtggg ttgcgccaaa tgcatctcgt
     1801 tcgcgcacgc aaatctggca gacaagtttt ttattctatc gaagatgacc acgtagcacg
     1861 gctatttacg ctgggtctcg atcacgttca gcacggataa cccatgcaca ctcattctca
     1921 tacccatatt ggcgatctta cccagcaaac caccaaacgt ctcaccctct ccttagttct
     1981 cactgcagca tttgttattg ttgagatcgt cgcaggtgtt tttggcaaca gtcttgcttt
     2041 gcttaccgat gcagcccata acttcaccga tgtcattgca ttaggcttaa gttggtatgc
     2101 cttacgcatc gccatgaaac ccgctcacgc tggcaagacc ttcggctatc accgtgttgg
     2161 gattcttgtc gcgcttatta actcgacgac gctaattctt atcgcctttg ggattttctt
     2221 cgaagcctat caccgcttct tgactccgcc cgaagttgat tcgactatcc tgatcggagt
     2281 tggcgctgtg gcttttatca tcaacctcgt caccgcatta atggtcaaag aaggcagcga
     2341 acatgatctc aacatgcgca gcgcctttct tcatctcatg ggtgatgtca tgtccacact
     2401 tggggcggtc attgcaggta tcatcatcgc cttcacaaaa tggaactggc tcgacccact
     2461 tgttagtgta ttgattggcg cgtttattct ttacaatgcc tggggcatcc tgaagcagac
     2521 cattcacatc ttgctagaaa gcactcccga aaacataaat gtagatagca tggtgcttga
     2581 cctgcaaaac atcaacggtg ttttaggcgt ccatgacctg catgtgtgga gcatcagtga
     2641 aaacttgcgc atgctctcag cgcatgtggt tatcggcgat actaccattg gcgaaggcgt
     2701 ctctattcaa caaaacatta atgaactgct tgcccatagt tacaatatcc atcacgccac
     2761 tttacaaatg gagtgtgaag gctgtacatc caaagacttg ttttgccaga tcactgaggc
     2821 acatcaacac gcccaccact aacttttatt tggagtccca tgtctcaagc cgcaaaagca
     2881 caacaacttt tatccttaca caacaacggt aaactgctca ttctgcctaa tgtgtggaat
     2941 cccattggcg cacggatgtt gcaagccaaa gggtgctcgg ctgtagccac agccagcgca
     3001 gccattgccg agtctcttgg ctacagcgac ggcgaacaac tcaaactgga taccatgcta
     3061 gatatggtcg ctcgtatcgt ccgcagtgta gacctgcccg tcaccgcaga ttttgaggca
     3121 gggtattccg atagtataga aggtttacaa gaaaatattt cacggttgct cgataccggg
     3181 gctgtcggct tgaattttga agatagcttc gacgacagcg cccacctacg ccccatccct
     3241 gaacaagtgg agcgcatcaa agctgtccgc gaggcagcga atcgaaaggg cattcacctt
     3301 gtgatcaacg cccgagccga tagtttcttc gcggaaggtt tttcatcaga tgaagaacgc
     3361 cttgatgatg tcatcacccg ccttactgcc tatacagcag caggcgctga ttccgtctac
     3421 ccggttgggc gtggcgataa agagactctc accgtcctgc gtaaacgact ctctgccccc
     3481 atcaacgtgc ttgccacagc taagactgcc agcttgaagc aacttcaaga gcttggcatc
//
LOCUS       DAOQFT010000242         3539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15076_length_3539_cov_8.903559, whole genome shotgun sequence.
ACCESSION   DAOQFT010000242 DAOQFT010000000
VERSION     DAOQFT010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15076_length_3539_cov_8.903559"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="pdxT"
                     /locus_tag="PLR93_07175"
     CDS             <1..444
                     /gene="pdxT"
                     /locus_tag="PLR93_07175"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNH26784.1"
                     /translation="GESTTIGKLAVAYNLIDPLREFGKSHAIWGTCAGAIFLSNDIGR
                     DQPLLGLMSIKVKRNAFGRQVDSFETDLEIEELYKATKTKHPYHAVFIRAPIIESVSG
                     DARVLSALEDGRIIAAQQGHLLATSFHPELTDDTRFHEYFVSLAS"
     gene            480..1397
                     /locus_tag="PLR93_07180"
     CDS             480..1397
                     /locus_tag="PLR93_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26785.1"
                     /translation="MTIRPLDLLDLPLIARYRNDVLTLDSTRALTRGHPLGAMGLLAY
                     INPARHLYAAVSDNEGVTLLGGVIHTRGEPFAKLLYLAPSQQLDTPQLTALIEHLTAQ
                     AGEWQAFHVIAEVDETSDVFIALRKAGFSVYAWQRVWNASLIQKTESNPGTGWSRVKS
                     IDLPAIQRMHYQIVPQLLHLVDPAPKLPNGFIHTDGYCFASVSYGVYGVVLTPWILPD
                     EGDVSEKIVSLIASLPDRRGRPVYLNVRSYQAWLEPVLEDLGAKAAPRQAVMVKYLSR
                     LVKEGVPARVVPSNARIQPTQMSRMDTDE"
     gene            1390..1710
                     /locus_tag="PLR93_07185"
     CDS             1390..1710
                     /locus_tag="PLR93_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26786.1"
                     /translation="MSSWSIYNKGSTIGTLSAEGGLILSDEAHDGGARITLKRGTNYV
                     SVSLHILGWMDHTRFFANDADAQREYRNMRSGVLTVLDVINTEGVSDIKIWEAISDFV
                     RRFP"
     gene            1712..2371
                     /locus_tag="PLR93_07190"
     CDS             1712..2371
                     /locus_tag="PLR93_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNH26787.1"
                     /translation="MLKALIFDFDGLILDTETPEVLVWQDIYKEHGFELPVEEWEKTV
                     GGYGISSFDPAHHLSHLSQGRLDPVSLKARYRLEADAIIHTKPIQPGIMDMIGSAKEN
                     GLKVAIGSSSPHSWVDTHTQRLNIYDQFDHIICQDDVAPGRTKPHPDIYLKALETLQV
                     DKAEAVVFEDSPNGVLASKRAGVFVVAVPNPLTARMNVQGDLTVSSLADLTLQDLINR
                     H"
     gene            2386..>3539
                     /locus_tag="PLR93_07195"
     CDS             2386..>3539
                     /locus_tag="PLR93_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26788.1"
                     /translation="MRKRIIPIFIVILALAGIVYVWNNSNQPAEPQATPTEEAFIVPT
                     VVSAHAPTVLPLANSPLKSSITSAGDYLVRQQLANGELAYQVNLLTNDRASTPSYIRL
                     MGGTSALYTVCRVAEDTVYCQAADRALEHYLPNLLTDPDHFKGTCLYSNGVCPLGGAT
                     VTIDAIYKRWQATNSVILNDHDLLATAVDLGYFIVSMRRPEGGFYHSFDPHFAGTVDP
                     SYVDPSFNGEAVAALLQLYEMTNNEFWLEQAHDVNTFMLTQPVTEDYAHSYAFALFAR
                     LDELSQADQDYAKQIADLIIAGQIRSLNPANSSVATATKIEALSSIAQALTLSNNKPE
                     GLDREIITFVTFVQARQFPANDCNFGLDGSVLEEYKGGLFNTCDDPSIRV"
BASE COUNT          855 a          873 c          887 g          924 t
ORIGIN      
        1 ggcgagtcga cgacgattgg caaattggca gtggcgtata acttgatcga cccgttgcgc
       61 gagtttggaa agtcacatgc gatttgggga acttgcgcgg gtgccatctt tctctcgaat
      121 gatattggac gtgaccaacc tttgctcggt ttgatgagta tcaaagtgaa gcgcaatgcg
      181 tttggacggc aagtcgattc gttcgagacc gaccttgaga tcgaagagtt gtataaagcc
      241 accaaaacaa agcatccata tcatgcggta ttcatccgtg cgccgatcat tgaatcggtg
      301 agcggagatg ccagagtgct ttctgccttg gaagatgggc gcatcattgc cgcacaacaa
      361 gggcatttgc tggcaacatc cttccacccc gagttgaccg acgatacgcg cttccatgag
      421 tattttgtct cattagcatc gtgatatatt tgtaggggcg caaccttcgc gcccctacta
      481 tgactattcg tccactcgac ctgttagacc tgcccctcat tgctcgttat cgaaacgatg
      541 ttttgactct cgatagcacg cgtgccctca cacgcggaca tcccctcggt gcgatgggat
      601 tgttggcgta tatcaacccg gcgcggcatt tgtatgcggc ggtcagtgat aacgaaggtg
      661 tgaccttatt aggcggagtg attcacaccc gtggcgaacc ttttgccaaa ttgttgtacc
      721 tcgcgccttc acagcaactt gataccccac aacttacagc attgatcgaa catctcaccg
      781 cacaggctgg ggaatggcag gcatttcacg ttatcgcgga agtggacgag actagtgatg
      841 tgttcattgc attgcgcaag gcaggtttct ccgtctatgc atggcaaagg gtttggaatg
      901 cgagtctgat tcagaaaaca gaatcaaacc ccggcacggg atggagccgg gttaagtcca
      961 tagacctgcc agctattcaa cgcatgcatt atcagatcgt cccacagtta ttgcatcttg
     1021 ttgaccccgc accgaaactg ccgaacggat ttattcatac tgatgggtat tgttttgcga
     1081 gtgtcagtta tggtgtgtat ggcgtggtgc tcaccccgtg gattcttccg gatgaaggcg
     1141 atgtgagcga aaaaattgtt tcactgatcg caagcctgcc agatcggcgc gggcgcccgg
     1201 tgtatttgaa tgtgagatcg tatcaggctt ggcttgagcc tgtgttggaa gacctcggtg
     1261 caaaggctgc gccgcggcaa gccgtaatgg tgaaatattt atcgcgcttg gttaaagagg
     1321 gtgtgccagc tcgtgtggtg ccctcgaatg cgcgcattca acccacccaa atgagtagaa
     1381 tggatacgga tgagtagttg gtcgatttat aacaaaggta gcaccattgg cacacttagt
     1441 gcggaaggtg gtttgattct ttcagatgaa gcgcatgatg gtggcgcacg gattacgctt
     1501 aagcgtggta cgaattatgt ttcagtttca cttcacattc ttggttggat ggatcacacc
     1561 cgattttttg ctaatgatgc cgatgcgcaa cgtgaatatc gcaacatgcg ttcgggcgtt
     1621 ctcaccgtgt tggatgtgat caacacggaa ggtgtaagcg atatcaagat ttgggaagca
     1681 atttcagatt ttgtcaggcg gtttccataa tatgttgaaa gcactcatat ttgatttcga
     1741 cgggttgatt ctcgataccg aaacacccga agttttagtt tggcaggaca tttacaaaga
     1801 acatggattt gaacttcccg tggaagaatg ggaaaaaacc gtgggcggat acggcatttc
     1861 aagctttgac cccgcccacc atctttcgca tctttctcag ggacggctgg atcctgtttc
     1921 cctcaaagct cgctacaggc tggaagcgga tgcgatcatc cacaccaagc caatccaacc
     1981 gggcatcatg gatatgatcg gctcggcaaa agagaacggc ttgaaagttg ccattggctc
     2041 tagttctcca cattcttggg tagatactca tacacagcga ttaaacatct acgaccagtt
     2101 cgatcacatc atctgtcagg atgatgtagc tcccggacgc accaaacctc accccgatat
     2161 ttatttgaaa gctctggaaa ccttgcaagt agataaagcc gaagcggtgg tctttgaaga
     2221 ttcaccgaat ggagtcttgg catctaaacg cgccggagtt tttgtggtgg ctgttccaaa
     2281 cccattgaca gcaaggatga acgtgcaagg cgacctgacc gtttcttctt tggcagacct
     2341 aaccttgcag gacttaatca atagacacta gttgattgtc gatttatgcg gaaacgaatc
     2401 attcccattt ttattgtgat ccttgctttg gctgggattg tctacgtttg gaacaactcc
     2461 aatcaacctg cagaaccgca ggcgacaccc actgaagaag cgtttatcgt gcctacggtt
     2521 gtttccgcac atgcgccgac agttcttcca ttggcgaaca gcccgctgaa atcatcaatc
     2581 acttcggcag gagattatct tgtgcgtcag caattggcga atggtgagtt ggcgtatcaa
     2641 gttaatttgc ttacgaatga ccgcgcctct acaccgtcgt atatccgctt gatgggtgga
     2701 accagtgcct tgtacacggt ctgccgtgtc gcagaggata cagtctattg ccaagctgca
     2761 gaccgagcct tggaacatta tctccccaac ctcttaacag acccagacca tttcaaaggg
     2821 acatgtcttt attcgaatgg ggtttgtccg ctgggcgggg cgacggtcac cattgatgcc
     2881 atctacaaac gttggcaagc cacaaacagc gttatcctca atgatcatga cctgctcgct
     2941 acggctgtag accttggtta tttcatcgtc tccatgcgcc gacccgaggg cggcttctat
     3001 cactcctttg acccgcactt cgccggaaca gtggacccca gttatgtgga cccctcattc
     3061 aatggcgaag cggttgcggc tctgttacag ctttatgaaa tgacaaacaa cgagttttgg
     3121 cttgagcaag ctcatgacgt gaatacattc atgctcaccc aacctgtgac agaagattac
     3181 gctcacagtt atgcatttgc tttattcgcc cgtttagatg aacttagcca ggcagaccaa
     3241 gattatgcca aacagatcgc agacctcatc attgccgggc agatccgctc gttgaacccc
     3301 gcaaactcca gcgttgcaac cgccaccaag atcgaagcct tatcgtccat tgctcaggcg
     3361 ctgaccttat ccaataacaa acctgaaggg ttggaccgtg agatcatcac ctttgtcact
     3421 tttgtgcaag cacgccaatt ccctgccaat gattgtaatt ttggtttgga tggttctgtg
     3481 cttgaagaat acaaaggtgg tctctttaac acttgtgacg acccatccat tcgtgtgga
//
LOCUS       DAOQFT010000243         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15097_length_3536_cov_6.722206, whole genome shotgun sequence.
ACCESSION   DAOQFT010000243 DAOQFT010000000
VERSION     DAOQFT010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15097_length_3536_cov_6.722206"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLR93_07200"
     CDS             <1..269
                     /locus_tag="PLR93_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26789.1"
                     /translation="TLQAFFFVAMRYMLDVIPTLSLLVIIGFWHGFELFKNKKVYIII
                     TTLLLIHTIACSLLISFSGNLETFKILNPELVREMTWTFNNLLK"
     gene            371..1399
                     /locus_tag="PLR93_07205"
     CDS             371..1399
                     /locus_tag="PLR93_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH26790.1"
                     /translation="MSRFLDFLNTADLNTLTQVSGVNRQLAGNLIAARPFDAEEDALN
                     VKGMGKATLARLQSFAEAQGNGSESSAMVPVEEEAMPAVMEKNPPAQEDAPKKESFFV
                     RLGRAIRSFFVALLKLIMLAILFVSIGAMFYYGLPYIQRTFIAPVEQNTAQIKTMEAE
                     IETLQTQLDEMNSRVSALEGSVEAHTASIEKLEEMQTALETQLEENQDAVLLELKQEI
                     MFTRALDVLARARLYLAQSNFGLAKTDVQTARDLLAELNAEKEDEVLTKAIERIDLAL
                     DNLPDFPVVASGDLEIAWQILVSGNAPASTATATPTLVPTEPATATPTAVDTAATPTL
                     IPSATATP"
     gene            1498..2928
                     /locus_tag="PLR93_07210"
     CDS             1498..2928
                     /locus_tag="PLR93_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNH26791.1"
                     /translation="MAYKIKFGTDGWRGVIAEDYTFDNLRRCAQGFASYMLSQGKKGQ
                     WVVVGHDKRFHSENFAAAVAEVLAGNGLKVYLTDGATPTPVIAYAVVDKKAAGAVNIT
                     ASHNPPTDNGFKVRNETGGAIDPEGLKQIEAGIPDDLKDVKLKNYKEAEAAGEIVKFD
                     AATPYIEHLTRDGLIDLQPIKDAGLTVVVDAMWGNGAGWFTRLLGGGKTKIIEIHNER
                     NPSFPEMKRPEPIRPNIDVGLKATVTNKADVLLITDGDADRCGLGDENGEFINQLRAY
                     ALLAYYLLEVRGERGDIVKTLSTTGMLNKLGEIYGVPVHETGVGFKYVAPKMTETNAL
                     IGGEESGGYAFRGNVPERDGILAGLYMLDFMVKTGKKPTQLLKDLFAKVGGEYYYDRV
                     DSPFSGDHETRKQIIRDNYPATLGGLKVTEVVTVDGFQFKLEDGGWMLIRFSGTEPIL
                     RVYTETRHADKVQAILQDGLKVAGIK"
     gene            3001..>3536
                     /locus_tag="PLR93_07215"
     CDS             3001..>3536
                     /locus_tag="PLR93_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26792.1"
                     /translation="MSGHFPPFGALGNLKSEHALSKRLQRTMLIFGGIWLCLLCIDIG
                     ISISVFNTSYEESNNALTALIVPIICVGIFALPGILILYQQWWSGVFNVAFYDLGISL
                     LTRHGVTDIQWRDIDSMWLEWAQSGTQSSPRTTLAAYAFRLKSGELFRLRSNMEGFNE
                     FEEKFYKPASAAILQTQM"
BASE COUNT          907 a          841 c          892 g          896 t
ORIGIN      
        1 tcaccttaca agccttcttc ttcgtcgcca tgcgctacat gttggatgtc attccgacac
       61 tatcccttct cgttatcatt ggcttttggc atggctttga acttttcaag aacaagaaag
      121 tgtatatcat catcactacg cttttattga ttcataccat cgcatgtagt ttgttgattt
      181 cgttctcagg caatttggaa accttcaaga ttctcaaccc agaactcgtc cgcgagatga
      241 cgtggacttt caacaacctt ctcaaataac agatatccac gcctcgtgcg tgacgtgggc
      301 ttgcatctat tacctcaagc tgattcgcat ctgaaacctt ttgttgtaca atgaccgtat
      361 aggagattcc atgagccgct ttctcgattt tctcaacact gcagacctga ataccctcac
      421 tcaagtatct ggcgttaacc gtcaactggc agggaatctc attgccgcgc gtcctttcga
      481 tgcagaagag gatgcgctca acgttaaagg catgggcaaa gcaacccttg cgcgcttgca
      541 gtcttttgct gaggcgcagg gaaatggttc agaaagcagt gctatggttc cggtcgagga
      601 agaggcgatg cccgccgtaa tggagaagaa tccgcctgca caggaagatg ccccgaagaa
      661 agaatcattc ttcgtgcgtt tagggcgggc aattcgatcc ttctttgtag cattgttaaa
      721 gctgatcatg ttggcgatct tgttcgtatc gattggcgca atgttctatt atggcttgcc
      781 atacattcag cggaccttca ttgcgcctgt agaacaaaac acggcacaaa ttaaaaccat
      841 ggaagcagag atcgaaacgc tccaaactca gctcgatgaa atgaactcac gcgtttctgc
      901 acttgagggc tcggtagaag ctcatacggc ttcgattgaa aaactggaag agatgcaaac
      961 cgcgctggaa actcaactcg aagaaaatca ggatgcggtt ctgttggaac tgaaacaaga
     1021 gatcatgttc acccgtgcgt tggatgttct agcacgtgca cggctgtact tggcacaaag
     1081 caattttggc ttggcaaaga ccgatgtgca aaccgcacga gatttgctgg ctgaactcaa
     1141 tgccgaaaaa gaagatgagg ttctaaccaa agccattgag cggatcgacc ttgcgcttga
     1201 caacctgcca gacttccctg tcgttgcctc aggagatttg gaaattgcct ggcagatttt
     1261 ggtctctggg aatgcacccg catccactgc aacggcgaca cccacgctag tccccacaga
     1321 gccagcaact gccactccca cagcagtaga tacggcggct acgcctacat taattccatc
     1381 tgcaacggca accccgtaac gcaaaactcc gaggcaatgc ctcggagttt ttttatgata
     1441 tttaggctcg tggcgggtat aatttcgaaa gttatcattc ccaagaggag aattctcatg
     1501 gcatacaaaa tcaaattcgg cacagacggt tggcgcggag tcattgctga agattacacg
     1561 ttcgacaacc tgcgtcgctg tgcgcagggg tttgcgtcgt atatgctttc acaaggtaaa
     1621 aagggacagt gggtcgtcgt tgggcatgac aagcgtttcc actccgaaaa ttttgctgca
     1681 gctgtggcag aagtgttggc tggcaacggc ttgaaggtct atttaaccga tggtgcgacc
     1741 ccaaccccgg tcattgctta tgccgtggtg gataagaagg ctgccggtgc ggtgaacatc
     1801 acggcgagtc ataacccacc cactgataac ggcttcaagg tccgcaatga gacgggtggc
     1861 gctattgacc ctgaaggttt gaaacaaatc gaagcaggca ttcctgatga tttgaaggat
     1921 gtaaagctca aaaattataa agaagccgaa gccgctggcg agatcgtcaa gttcgatgcg
     1981 gcgactccgt atattgaaca cctcactcgc gatggattga tcgatttaca gcccatcaaa
     2041 gatgccggtc tgaccgtggt ggtggatgcc atgtggggca atggtgcagg ttggttcacc
     2101 cgtctgctcg gcggcggtaa gaccaagatc attgaaattc acaatgaacg caacccttcc
     2161 ttccctgaaa tgaagcgccc ggaacccatc cgcccgaaca ttgatgtagg tttgaaagcg
     2221 actgtgacca acaaagccga tgtgttgctc atcaccgatg gtgacgcaga ccgttgtggc
     2281 ttgggggacg agaacggtga attcatcaac caattacgcg cctatgcgtt gctggcgtat
     2341 tacttgctcg aagtacgcgg tgaacgcggc gacatcgtca agacgctttc aacaactggt
     2401 atgttgaaca aactgggcga gatatacggc gtacctgtcc atgagacggg cgtaggcttc
     2461 aagtatgttg ccccgaagat gaccgagacc aatgcactca tcggcggtga agaaagcggc
     2521 ggctatgcct tccgtgggaa cgtgcccgaa cgcgatggca ttttagccgg cttgtatatg
     2581 ctcgatttca tggtgaagac cggcaagaaa ccgactcaat tgctcaagga cttgttcgct
     2641 aaagtgggcg gtgagtatta ctacgaccgg gttgatagtc ccttcagcgg tgaccacgag
     2701 actcgcaagc agattatccg cgataactac cctgccacat taggcggatt gaaagtgacc
     2761 gaagttgtta cggtggatgg cttccaattc aagcttgaag atggcggctg gatgttaatc
     2821 cgcttctcgg ggacggagcc gatcctgcgc gtatataccg aaacaagaca tgctgacaaa
     2881 gtgcaagcga ttttgcaaga cgggttgaag gttgctggga ttaagtaagt aaagtgtgtt
     2941 caaagtgtca ggtcttttat gacctgacac ttttttatta agcaaaagga gtttatcggt
     3001 atgtcaggtc attttccacc ttttggcgcg ttaggcaact tgaaaagtga gcacgcttta
     3061 tcaaagcgat tacaaagaac catgctgatc tttgggggaa tttggttatg cctactatgt
     3121 atagatatcg gcatttcaat ttctgttttc aacacaagtt atgaagagtc taataacgct
     3181 ctcacggctc ttatcgttcc aataatatgt gttggcattt ttgctttgcc tggaatattg
     3241 attctctatc aacagtggtg gtcaggtgtg ttcaatgtcg cgttttacga tctagggatt
     3301 tctctcctca cccggcatgg agttacagac attcagtggc gcgatattga ctccatgtgg
     3361 ctggagtggg cgcagtctgg gacgcaatct tccccaagaa caacccttgc cgcctatgcc
     3421 ttccgcctca aaagcggaga attattccgt ctacgcagca atatggaagg gttcaacgaa
     3481 tttgaagaaa agttctacaa gcctgcttct gctgccattt tacaaactca aatgga
//
LOCUS       DAOQFT010000244         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15264_length_3510_cov_8.402026, whole genome shotgun sequence.
ACCESSION   DAOQFT010000244 DAOQFT010000000
VERSION     DAOQFT010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15264_length_3510_cov_8.402026"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1044
                     /locus_tag="PLR93_07220"
     CDS             112..1044
                     /locus_tag="PLR93_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26793.1"
                     /translation="MTDEPIKEDPVQKFRKLIASFIPLSRLPKKSTQAEPKEELPVEE
                     IKEEVKVEENKKDALSALPKKSTPQNDMLGSLPKKGALPPKKAEPKPVETLASAPSPA
                     STPTSKLVPASSSGYSVWGPRLWTTASVISLTINIILMIVLIILVISVYRLKLDMAKI
                     MDASTSVMGLPAGLYSNFEKMERASIQTNVEVNTSIPVKFDLQLNQQTNVVLSQDVTI
                     QNARVTVNTGGLNISQANTTIVLPAGTTLPVNLALTVPVDQQVPVTLNVPVDIKLKDT
                     QLNDPFVGLQEVIKPLYCLLEPTAINMDGNPICQ"
     gene            1104..2366
                     /locus_tag="PLR93_07225"
     CDS             1104..2366
                     /locus_tag="PLR93_07225"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH26794.1"
                     /translation="MHKNITQVQLKNGMKIMLKEIHTAPIISSWIWYKVGSRDESTGK
                     TGISHWTEHMMFKGTKKFPGGILDKAISREGGRWNASTSRDSTRYYETMPAAKIDLAL
                     RLESDRMTKSIFDAKEVASERTVIISEREGSENEPMFSLGEAVQHASFRVHPYHHEII
                     GDMADLRTITRDDLYNHYRSYYVPNNAVLSLAGDFDTKSMLKRIKELFEPIPKGNVPP
                     RLARPEPERKGELRLTVEGPGETAFTQVAYPFPNATHPDYFALQVLESLLNGASGLSY
                     KTCRLYRALVDKGHVVDISGWSESTIDPFLYRITLVHRPDKKPEEALAVLDDEIKKVQ
                     DKPVSAAEIQRAVKQARAVFAYGSENITHQAFWMGYTSMFSDYSWYTTYLDKLAKVTP
                     KDVQRVAQIYFQTSKRVVGNYIPKGKAA"
     gene            2366..>3510
                     /locus_tag="PLR93_07230"
     CDS             2366..>3510
                     /locus_tag="PLR93_07230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH26795.1"
                     /translation="MATKTKSSMPSPEDVYQTTLPNGITVLARSNYNSPSINVGGYLP
                     AGAIFETDEKLGLADFVTAMLMRGTQTRTFDSIYNELETVGASLGFDSGVHNASFGGR
                     ALVEDLPLLMKLLSESLRTPTFPKDEMERLRTQILTGLALRAQDTSDMADLLYEQFLY
                     ADHPYGKPSDGYVETIQAISRKDLETFHRLHFGPRGMVITIVGAIQPKKAVEAVAKFL
                     GDWTVPSQKEPPVLPPLKPLKKTVRKHHQILGKSQSDLVVGTIGPTRKSPDFMAASLG
                     NSVLGQFGMMGRIGHVVREKSGLAYYAYSSLHSGVGPGSWEVAAGVNPKNLKKALDLI
                     DVELRRFVKNGVTKEELADSQANYVGRLPLSLESNGGMVSAILNIHR"
BASE COUNT          932 a          921 c          816 g          841 t
ORIGIN      
        1 ttttcagatg ttaattccgc attagagatg tttgtgcttg ctgtaagaaa cccaccccat
       61 cttcataaac atctatgcta atattaatcc atgtgctaac caagaggcag tatgacagac
      121 gaacccatca aagaagatcc tgtacaaaaa ttccgaaagc ttattgctag tttcattcca
      181 ctctcacgcc tgcccaaaaa atcaacacag gcagagccta aagaagaact acccgtggaa
      241 gaaataaaag aagaggtcaa agtggaagaa aataaaaaag acgctctttc agctttaccc
      301 aaaaaatcaa cgccacaaaa tgacatgctg gggtcgttac ccaaaaaggg agcgttaccg
      361 cctaaaaaag ctgaaccgaa accggtagag actctggctt ccgcccccag ccccgcctca
      421 accccaacct cgaaactggt acctgcttca agtagcggat actccgtttg gggtcctcgt
      481 ttatggacaa cggcaagcgt tatctctctt acgatcaaca tcattctgat gatcgttctt
      541 atcattctgg tgatcagcgt ctatcgtctc aaactagata tggcgaagat catggacgct
      601 tccacctccg tgatgggact tcccgctggc ttatattcca acttcgaaaa gatggaacgc
      661 gccagcattc agacgaatgt agaggtcaac acctcgattc cggtcaaatt tgacttgcaa
      721 ctcaaccaac aaaccaatgt cgtcttgagt caagatgtga ccattcaaaa cgcgcgtgtc
      781 accgtcaaca ccggcgggtt gaatatttcg caagccaaca ccaccattgt cctcccggcg
      841 ggcactacat tacctgtcaa cttagccctt accgtccccg tagatcagca agtgccagtg
      901 accttgaacg tccctgttga tatcaagctc aaagacacac aattgaacga cccctttgtt
      961 ggcttacaag aagtcatcaa accgctttac tgtttgctcg aacccaccgc catcaatatg
     1021 gacggcaacc cgatctgcca gtaatacttg cttgttgtgc accgtctgag gatgcaaaat
     1081 aacagaaaat actttggagt taaatgcata aaaacatcac gcaggtccag cttaagaatg
     1141 gcatgaagat catgctcaaa gagatccata cggcacccat catctcgtcg tggatctggt
     1201 acaaagtcgg ctcgcgcgat gagtccaccg gcaagaccgg catctctcat tggacagaac
     1261 acatgatgtt caaagggacg aagaaattcc caggcggtat tctcgataaa gccatctccc
     1321 gtgaaggcgg acgttggaac gcatccactt cccgcgattc cacccgctat tacgaaacca
     1381 tgcccgccgc caaaattgac cttgccctgc gcctcgaatc agaccgcatg accaagagca
     1441 ttttcgatgc caaagaagtg gcatctgaaa gaacggtcat catttcagag cgcgaagggt
     1501 cagagaatga gcctatgttc tcactagggg aggcggtgca acatgcttca ttccgtgtcc
     1561 acccgtatca tcatgaaatc atcggtgaca tggcagattt gcgcaccatc acccgcgatg
     1621 acctttacaa ccactatcgt tcatactatg tcccgaacaa tgctgtcctt tcgctggctg
     1681 gagattttga taccaaatcc atgctcaaac ggatcaagga actgttcgag ccgatcccga
     1741 agggaaatgt cccgccgcgc cttgcccgcc ctgaaccaga gcgcaaaggt gaacttcgct
     1801 taacggtgga aggtcctggc gagacggcgt tcacgcaggt ggcgtatccc ttcccaaatg
     1861 ccacacatcc tgattacttc gctttgcaag tgctcgaaag cttgctcaat ggcgccagcg
     1921 gactttccta taaaacctgc cgtttgtacc gcgccctcgt agataaggga cacgttgtag
     1981 atatttctgg ttggtccgaa tccaccattg acccgttctt atatcgtatt actttggtgc
     2041 atcgccctga taaaaagccc gaagaagcgc ttgcagtgtt agatgatgag atcaagaagg
     2101 tgcaagataa acctgtctct gcggcagaaa ttcaacgtgc tgtgaagcag gcgcgcgctg
     2161 tttttgctta tggcagtgag aacattactc atcaagcctt ttggatgggc tacacctcca
     2221 tgttctcaga ttacagttgg tacaccacgt atctcgataa gctagccaaa gtcacaccca
     2281 aagatgtgca gcgtgtggcg cagatttatt tccagacaag taaacgtgtg gtggggaact
     2341 acattcccaa agggaaggcg gcgtaatggc aactaaaacc aagtcttcca tgcccagccc
     2401 tgaggatgtg taccagacca cacttcccaa tggcattact gttttagcgc gctccaatta
     2461 caacagccca tctatcaatg tgggtgggta tcttcctgct ggcgctattt ttgaaaccga
     2521 tgaaaaactc ggtctggcag attttgtcac tgccatgctc atgcgcggaa cccaaacccg
     2581 cacgtttgac tccatttaca acgagttgga aactgtcggc gcgagcctcg gctttgactc
     2641 gggtgttcac aatgccagtt ttggcggtcg cgccttggtt gaagaccttc cgcttcttat
     2701 gaagcttctc tctgaatcgc tgcgcactcc cacgttcccg aaagatgaaa tggaacgtct
     2761 gcgcactcaa attctcaccg ggctggcact gcgtgctcaa gatacctctg atatggcaga
     2821 cttgctctat gagcaattcc tgtatgccga tcatccctat ggcaaacctt ctgatggata
     2881 tgtggagacc attcaggcga tctcacgcaa agatttggaa accttccatc gcctccactt
     2941 cggacctcgc ggcatggtca ttacaatcgt gggcgctatc cagccgaaaa aagcggttga
     3001 ggcggtggca aaattcttgg gcgactggac agttccaagt cagaaagagc cgcctgttct
     3061 tcctccgctt aagccgttga agaaaactgt ccgtaagcat catcaaatcc tcggcaaatc
     3121 ccaatcagat ttggtcgttg gcacgattgg acccacacgc aaaagccctg atttcatggc
     3181 ggcgtctctt gggaattcgg tcttgggtca gttcggtatg atggggcgca ttgggcatgt
     3241 agtgcgtgaa aaatcgggct tggcatatta tgcttattca agtctgcact ctggggtagg
     3301 accaggcagt tgggaagtgg cggctggagt caacccgaag aacctgaaaa aagcgcttga
     3361 cctgattgat gttgaactgc ggcgcttcgt aaagaatggc gtgactaaag aggagcttgc
     3421 tgatagtcag gcaaattatg tgggacgttt gccgctttca cttgagtcga atggcggtat
     3481 ggtgagcgca attttgaata ttcatcgcta
//
LOCUS       DAOQFT010000245         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15266_length_3510_cov_4.650362, whole genome shotgun sequence.
ACCESSION   DAOQFT010000245 DAOQFT010000000
VERSION     DAOQFT010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15266_length_3510_cov_4.650362"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLR93_07235"
     CDS             <1..518
                     /locus_tag="PLR93_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH26796.1"
                     /translation="PLGFSLDVFEVFLKGLVKRGKSVWVDTSGTALKTALKLHGLNIK
                     VNAAELSDALGMKISNADQAIFACNQLLNDGISSAAVTLGKDGAVLVEHSEIWVAYPP
                     VIKVVSSVGSGDAFLGGLAVALDRGHPLDVSLRHAVAAGAANALHFGGGKLSREEFER
                     IRQDVTILPYM"
     gene            651..1505
                     /locus_tag="PLR93_07240"
     CDS             651..1505
                     /locus_tag="PLR93_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH26797.1"
                     /translation="MVTLFGKHIRLFRPHTIWIRRIVQWVFFLLIASIAVSHTLAESG
                     MEIPILSGASIHAVCPFGGVETLYTFATSGLFVRKIHDSSLVLAGIVLLLSILFGPVF
                     CGWVCPLGTVQEWVGKLGRKIFKRRYNHFVPVKVDRILRFARYGILIWVLYVTAKSGT
                     LVFEAYDPYFALFNFWSTEVVWSAIAILIATLTLSVFVERPWCKYACPYGAVLGITNL
                     FRVFSIKRSELTCKADGACSIMCPMNIQVDTQTVIRDHQCISCLECTSEAICPVAKTV
                     VFTSGGVK"
     gene            1502..2248
                     /locus_tag="PLR93_07245"
     CDS             1502..2248
                     /locus_tag="PLR93_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26798.1"
                     /translation="MMLTSKPLAVLVFVILFGGIFFSSAMGWWATESTKEPAAFTEGE
                     FAGLANPADIRGSYTFGDIANSFEVSPDLLAQAFGVTQDNPSEFAVNELEAIYLESGF
                     EIGTASVRLFVAYYTGLPFDTTGQEIYMPTSATDILLAKGNLSPEQIAYLEVYTVSVN
                     PTTLIEPPSLESTPIPVDSSIIEEYTIKGKTVFGDLITWGVPQATIEQIIGAPMPDPA
                     MKIKDYATANGLEFETLKTSLQSEVDKVQP"
     gene            complement(2308..2868)
                     /locus_tag="PLR93_07250"
     CDS             complement(2308..2868)
                     /locus_tag="PLR93_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26799.1"
                     /translation="MNLTVHPLTSDLFPSLEDLFGDRGPVSRCWCMYWRIGADYLKHP
                     RHENKAEFRKIVEVGPPPGLLAFDGEIPVGWCQLTPRDALPWMDKTWRLKRVDDLPVW
                     SLSCFYIRKGYRKKGVTSALIRAAISIAAQAGVPALEAYPLDGSLSPSATSTGYVTTF
                     ERLGFKVVARHFMPRPIMRYELKKKV"
     gene            3209..>3510
                     /locus_tag="PLR93_07255"
     CDS             3209..>3510
                     /locus_tag="PLR93_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH26800.1"
                     /translation="MSFLNTFTENARKYPEKVALEFIDPPLQRITYAELDRLIDQTAL
                     YLQSLGLQPGDRVALQLSKSLEFILLHLATIRLGAITLPLNPAYPADELKYFLKDSG"
BASE COUNT          878 a          859 c          844 g          929 t
ORIGIN      
        1 tgccattagg gttttcattg gatgtttttg aggtcttttt aaaaggtctt gtaaaaagag
       61 gaaagtctgt atgggtggat acaagtggca cggcattaaa gacagcactg aaactacacg
      121 gtctgaacat caaggtcaat gctgctgagt tgagtgatgc cttaggaatg aaaatttcaa
      181 atgcagatca agcgatattt gcatgcaatc aattgttgaa tgatggtatt tcgtctgccg
      241 cagtgacact tggaaaagat ggggcagtat tggttgagca ttcagaaata tgggttgcat
      301 acccgcccgt aatcaaagtc gttagcagcg ttggcagtgg agatgctttt ttgggcggct
      361 tggcggtcgc gcttgataga gggcatccgc ttgatgtatc tctgcggcat gccgtagcag
      421 caggtgcagc aaatgccctc cactttggcg ggggaaagct ctctcgtgaa gaatttgaaa
      481 gaatacgtca agatgtaacg attttgcctt atatgtaatt gttcgcatta tcgaacaggt
      541 gggaaatgac cctgtccaca atttttcttt accaaatctt taagcaacat ttgaatctcc
      601 tcaataatga gcaactacac ttcgggcaat taaatctccc ggaggttctc atggtgacac
      661 tatttggtaa acacattcgg ttgtttcgcc cgcatacgat ctggatacgc cggatcgtgc
      721 agtgggtctt ttttttgctg atcgcatcca ttgcagtcag tcacaccctt gctgaaagcg
      781 gcatggagat tcccatcctt tctggtgctt cgatccatgc ggtttgtccg tttggcggag
      841 tggaaactct ttataccttt gcaaccagcg gacttttcgt aaggaaaatt catgattcgt
      901 cgctcgtgct tgcggggatt gtgctgcttt tgtccatttt gtttggcccg gtcttttgcg
      961 ggtgggtctg cccattgggc acggtgcagg aatgggtggg aaaacttggc agaaaaatct
     1021 tcaaacgacg ctacaaccat tttgtgcccg taaaggtcga tcgtattctc cgctttgcgc
     1081 gctatggaat actaatctgg gtgctttacg tcaccgccaa gagcggaacc ctggtcttcg
     1141 aagcctatga tccatacttt gccttgttca acttctggag cacggaagtg gtctggagtg
     1201 ccattgccat tctgatcgcc accctgacgc tttctgtttt tgtagaacgc ccctggtgca
     1261 agtacgcctg cccatatggt gcggttttgg ggatcaccaa tctctttcga gttttctcga
     1321 tcaaacgctc agaactcacc tgcaaagccg atggcgcctg ttcgatcatg tgcccaatga
     1381 acattcaggt ggatacccaa acggtcatcc gcgatcatca atgcatttcc tgcctggaat
     1441 gtacatcgga agccatctgc cctgtagcga aaacagtcgt atttacgagt ggaggtgtga
     1501 aatgatgctc acatccaaac ccctggcagt ccttgtgttt gtgatcctgt ttggcggcat
     1561 tttcttctct tcggcaatgg gctggtgggc aacagagtcg accaaagaac ctgccgcctt
     1621 caccgaaggc gagtttgccg gtctggccaa cccggcagat attcgcggct catatacttt
     1681 tggagatata gcaaactcct tcgaagtttc tcccgatctg ttggcgcaag cctttggcgt
     1741 cacacaggat aatccgtccg aatttgcggt caatgaactt gaagcgatct atcttgaaag
     1801 cggtttcgag atcggtacgg cctctgtccg attgttcgtg gcatactata ccggcctgcc
     1861 cttcgatacg accggtcagg aaatctatat gccaacgtca gcgacggata ttctgttggc
     1921 aaaaggcaat ctctctcccg aacagatcgc atatcttgaa gtctatacgg ttagtgttaa
     1981 tccgacaact ctgatagaac cgccgagcct tgaatcaacg cccatccctg tagattcatc
     2041 gatcattgaa gagtatacga tcaaaggcaa aaccgtcttt ggtgatttga tcacatgggg
     2101 tgtgcctcag gcgaccatcg aacagattat cggtgccccc atgccggacc ctgcaatgaa
     2161 aatcaaagac tatgccaccg caaatggatt ggagtttgaa actctcaaaa cctccctgca
     2221 atctgaagtg gataaagtcc aaccgtaaat aacaaagcct gatctgacac acctggaaac
     2281 ctgtcagacc aggctttatc tctcgcctta tactttcttt tttaactcat agcgcatgat
     2341 gggacgaggc ataaaatgcc gggcaacgac cttaaaccca agccgctcaa aggtagtgac
     2401 gtaaccggta ctggtcgcac ttggcgataa gctcccatca agcggataag cctcaagtgc
     2461 aggtacacca gcctgcgcag caattgaaat tgctgcgcgg atcaacgcag atgttacgcc
     2521 ttttttgcgg tatcctttgc ggatataaaa acaagagagt gaccataccg gcagatcatc
     2581 cacccgcttg agacgccagg ttttatccat ccagggcagg gcatcgcgcg gagtcaattg
     2641 gcaccagcca actggaatct ccccatcaaa agcaagcagt cccggcggcg gcccaacttc
     2701 gacgatcttg cgaaattccg ctttattctc atggcgcgga tgtttaagat aatctgcacc
     2761 gatgcgccag tacatgcacc agcaacggct gaccggcccg cgatcaccga agaggtcttc
     2821 aagcgacggg aacagatcag atgtgagtgg gtggacagta agattcacgt tatattggga
     2881 tcaacgaccc catcgcccga ccataatccc aaccatccat ggagggaggt tccacaccca
     2941 gggcagtcaa catgcttttg atctgttcgc ggtgttcggt ggcgtgattg agaggtattc
     3001 atctgataga gaaaggcaaa gctcaatgat cttttcattt gcccaattgt tatgttcgaa
     3061 tattctggca agagaatcct cagtcatatt cagactcctt cagttaaata aattttgaaa
     3121 accgaatacg atgaacactg tcgatattat acgcgaacga aaataactcg tgctggcacc
     3181 cgtatatcca gaccgatata ataggcaaat gtctttcctg aacactttta ctgaaaacgc
     3241 aaggaaatat cctgaaaagg ttgcattgga atttattgac ccgccgttgc aacgtatcac
     3301 ctatgcagaa ttggacaggt tgatcgacca gacagccctt tatttgcaaa gtcttggcct
     3361 gcaacccggt gaccgtgtgg ctttgcagct ttccaaatcc cttgaattta ttcttttgca
     3421 tttggcaacc atccgcctgg gagccattac cctgccgctt aatcccgcct acccggcgga
     3481 tgagttgaaa tacttcctca aagactcggg
//
LOCUS       DAOQFT010000246         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15358_length_3497_cov_5.191458, whole genome shotgun sequence.
ACCESSION   DAOQFT010000246 DAOQFT010000000
VERSION     DAOQFT010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15358_length_3497_cov_5.191458"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLR93_07260"
     CDS             complement(<1..227)
                     /locus_tag="PLR93_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNH26801.1"
                     /translation="MLNIISKMEINQPVPDFELPDLNGALHRLSNYRGRIVIVNFWSC
                     ECPHSERTDKAILSMFVQWQDDVTMLTIASNR"
     gene            289..1731
                     /locus_tag="PLR93_07265"
     CDS             289..1731
                     /locus_tag="PLR93_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNH26802.1"
                     /translation="MDGLFLALCVLYILSGIPLVPFHGDESAYIILSEDYDRVFKKHE
                     VEKVLFDPDGPKQYLRLSTGAILSFSIGFARDITNNEDPINKWLWGASWDENLAKGNL
                     PAPSLLNVARFSSAIMGALGTVLFFVLLRCLYPSRLAAWLATLAFVTQGSLLVSIRRA
                     MQEGPKFLFLTLTTLLAFLIIQNLQTQKLRKALYFALGIASGLTLAAKQDTAPVLVAL
                     YLTIAFIPVWNRTSFRAVWHTLLYLFAATLLAYATFLSFMPVFWQWWESVFVLTGLSV
                     ALFQIPFIHETRSKMLASAGLALVVVMTFVSPALWGKLHIPLSSMAELRQSIVGSQVE
                     VSNGQDVLKADSAMGRLNFLLENTISSKVMYMEVDSFNVPPMQASIKAYEASFLSGRT
                     GSFVFDGLIFLLALLGMWFLLRNFNSTSLLVYLLFFVTGTFLLVAIPLAWQRYFIIMQ
                     LPYMLFAGAGVYQLEMWLKPLILEKKTASA"
     gene            complement(1756..2181)
                     /locus_tag="PLR93_07270"
     CDS             complement(1756..2181)
                     /locus_tag="PLR93_07270"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNH26803.1"
                     /translation="MGKSVTHVGEAGAGQVAKAANQIMVAAQMVAMGELLVFAKKAGV
                     DPRKVVDAIKGGAAQCWTLDVKPPRLFDGNRNPGFKAHMQLKDMGIVLDTAKEYDIPI
                     SSAEEHTKFFKMMVEQGMGELDNAAVVGVIEKLAGVEIP"
     gene            complement(2167..2649)
                     /locus_tag="PLR93_07275"
                     /pseudo
     CDS             complement(2167..2649)
                     /locus_tag="PLR93_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            complement(2776..>3497)
                     /locus_tag="PLR93_07280"
     CDS             complement(2776..>3497)
                     /locus_tag="PLR93_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNH26804.1"
                     /translation="YFAFTILAVLALVLGACGPTTINQAAPTTDRIMNVNGLGVVYLT
                     PDIAYINIGVGTQKPDAAEAVEANKAQTTKVIQAIKDSGVDDKDIRTTNFSIWSNPQY
                     DPAGTGQILSTSYSVDNTVNVTIRDLDKLGDLLDAAVQAGANNIYSIQFDVDNKNDAN
                     KEARTLAVENARMQAQELAAATGVELGEIQSISYYESSPTQYFEGKGGGGGGDVASAA
                     VPIQPGQLAISVNVNITYFIK"
BASE COUNT          821 a          822 c          845 g         1009 t
ORIGIN      
        1 cgattcgacg cgatagtcaa catcgtcaca tcatcctgcc attgtacgaa catggacaga
       61 atggctttat ctgtccgctc ggaatgcgga cattcgcacg accaaaaatt gacaatcaca
      121 atgcgcccgc gataattcga aagacgatgg agggcgccat taaggtcggg caattcaaaa
      181 tcagggacgg gttggttaat ttccatttta gatataatgt tcaacatggc gatcataaag
      241 gaatttataa gaaaaatcca tgctcagcag gaaaaacata aaacatggat ggacggtttg
      301 tttttggcgt tatgtgtact ctatatcctt agcgggattc ctttggtgcc atttcatggg
      361 gatgaatctg cttatattat tttgagcgaa gattacgata gagtctttaa gaagcatgaa
      421 gtagaaaagg tgttgttcga cccagatggt cccaaacaat acctgcgatt atctacgggg
      481 gcaatattgt cattttcaat tggcttcgca agagatatta ccaacaatga agacccgatc
      541 aacaagtggt tgtggggggc atcttgggat gaaaaccttg ccaaggggaa tctccctgca
      601 ccaagcttgt tgaatgttgc caggtttagt tctgcaatta tgggcgcttt gggaactgtg
      661 ctgttttttg tgcttttgcg ttgtttgtat ccgtctcgct tggcagcctg gctggcaacc
      721 cttgcctttg taacgcaagg cagtcttttg gttagcattc ggcgggcaat gcaagaaggt
      781 ccgaagtttt tgtttttgac cctgacaacc ttgcttgctt ttttgatcat tcagaatctt
      841 caaacgcaga aactgcgcaa agccctatac tttgctttag gcatcgccag cggattaacc
      901 cttgccgcca agcaagatac agctccggtt ttggtggcgc tctatttgac gattgcgttc
      961 ataccagttt ggaacagaac atctttccga gctgtttggc ataccttgtt atatcttttc
     1021 gctgccaccc tgcttgcata tgcaaccttt ctttctttta tgcccgtttt ttggcaatgg
     1081 tgggaaagtg tttttgtatt aactggtctt tctgtagcct tgtttcaaat tccatttatc
     1141 catgagacgc gttcgaaaat gctggcaagt gccgggctgg cattggtggt ggtgatgaca
     1201 tttgtatcac cggcgttatg ggggaaatta catatcccat tgagcagtat ggcagaatta
     1261 cgccaatcga ttgtagggtc tcaagtggag gtttcaaacg ggcaggacgt tcttaaggcg
     1321 gattctgcaa tgggtaggct gaatttcctt cttgaaaata ccatttcatc caaggtgatg
     1381 tatatggaag tggatagttt taatgtgccg cctatgcaag caagcataaa ggcgtatgaa
     1441 gcttcattcc tgagcgggcg caccggctct ttcgtgttcg acggacttat cttcttgctg
     1501 gcgcttttgg gaatgtggtt tcttttgcgc aatttcaatt cgaccagtct tctggtttac
     1561 ctcttgtttt tcgttacagg cactttttta ctggttgcaa ttccgctggc atggcaaaga
     1621 tatttcatca ttatgcaact tccttatatg ctttttgcgg gcgctggcgt atatcaacta
     1681 gagatgtggt tgaagccctt aattctggaa aagaaaacag cctctgcgta gaggctgttt
     1741 ttgtaaaagc ttttattagg gtatctcaac cccagccaat ttctcaatca cacctaccac
     1801 tgcggcattg tccaactcgc ccataccttg ttcaaccatc atcttgaaga acttggtgtg
     1861 ctcttctgcc gatgagatag ggatatcata ttcctttgct gtatccaata cgatgcccat
     1921 atcttttaat tgcatgtgcg ctttgaaacc agggttgcgg ttgccgtcga acaaacgcgg
     1981 tggcttgacg tccaaggtcc agcattgagc cgcgccaccc ttgatggcat ccactacttt
     2041 acgtgggtcg actcctgctt tcttggcaaa gaccaataat tcgcccattg ccaccatttg
     2101 ggctgccacc atgatctgat tggcagcttt ggcaacctgc ccagctcccg cctcgccgac
     2161 atgggtgacc gacttgccca tggcttgaaa gacgggcatt actttttcaa gcgcttcggc
     2221 ttcgccgccg accataatag tgagagtacc attcttggca ccaatatcgc caccagagac
     2281 aggtgcatcc agagagaaaa tccctttttc ttttaacttc gccgcgatca agcgagccga
     2341 cgccggtttg atggtggagt tatccaccac gatcgtgccg ggttttgcgc ctgcaatgat
     2401 tccattgact tcatccaatg ccactttttc tacatcggga gagtcgggta aatttgtaaa
     2461 aacaatatcc acttgttcgg caacttcttt gggcgaacct gccgcagtag cgccttcggc
     2521 gaccaattca tccaccgcag cgcgtgagcg attatgaaca actaccggaa agcccgcctt
     2581 cataatattc cgcgcaatgg atttgcccat taatcctaaa ccgatataac caactcttaa
     2641 catgaagaga ctccttgaat gagaatggtg agattataac ggcactgcca gatttgccgc
     2701 gatctacgaa aaggtagagg atgtaaaaag cctgcctctt tgcagagaca ggcttcaggt
     2761 ccgaacttga ggcgtttact taataaagta ggtgatgttt acattgacac tgatggcaag
     2821 ctgacccggt tggatcggta ccgcagcgct ggcaacatcc ccgccaccac cgccaccctt
     2881 gccttcaaag tattgggtgg ggctgctttc gtagtagcta atggactgga tttcacccaa
     2941 ttccacaccg gtcgcagcgg caagttcttg tgcttgcatt ctcgcgtttt ccacagccaa
     3001 ggttcgggct tctttgttgg catcgttctt gttatccaca tcgaactgaa tgctgtagat
     3061 gttgtttgca ccggcttgca cggcggcatc gagcaggtcg ccgagtttat ccaggtcacg
     3121 aatggtcaca ttgacggtgt tatccacaga gtagctggtg ctaagaatct gaccagttcc
     3181 tgccggatcg tactgcgggt tcgaccaaat gctgaagttg gtggtgcgaa tatctttgtc
     3241 atccacaccc gagtctttga tcgcctgaat gaccttggtg gtttgtgctt tgttggcttc
     3301 cactgcttcg gcggcatctg gcttttgagt gccaacgcca atattgatat aagcaatatc
     3361 gggggtaagg tacacaacgc ccagcccgtt gacgttcata atgcggtccg ttgtaggggc
     3421 ggcttggtta atggtggtcg gtccacaggc gcccaagacg agtgccagaa ctgccaaaat
     3481 ggtaaaagca aaatatt
//
LOCUS       DAOQFT010000247         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15386_length_3493_cov_6.697499, whole genome shotgun sequence.
ACCESSION   DAOQFT010000247 DAOQFT010000000
VERSION     DAOQFT010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15386_length_3493_cov_6.697499"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="PLR93_07285"
     CDS             <1..1567
                     /locus_tag="PLR93_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNH26805.1"
                     /translation="TACEQAGARYAALGVLDSKGKLEKFISVGLTHDAIKRIAHPPVG
                     RGLLGELMDTEDPLRVRSIQEHPKSVGFPAHHPKMVSFLGVPIRTAEGQIGQIYLTEK
                     LDADEFSSDDEMIIQMLATYAATAIANARLIDQMKERDVALTRRNVDMTLLNDIASTL
                     TSSLELDEILNKTLGLVMNYMKVEAGEIFLLEEDKTTLRMMLHRGQAAEAFWTHNIFR
                     VGEGYPGVVAETCRPLICTDLANDPNFQREAVVKAGFHQVLSFPLVSGERVMGVMSIA
                     TRSKAAFEDRSVQMLTAVGAWAGLSIENARLHVNARRLAVLEERNRIGMDLHDGIIQS
                     IYGVGLALEGTKLLVDEDASSAKEKIDNAIDGLNQAIRDLRAYILDLRPRQLGTEGVI
                     SGIKRLIAEYRANTFSEVTFMPPDSDLKELTQTQALALFHICQEALANAAKHAKAKNV
                     QISLWTTEDRALLELHDDGKGFNTDEMSTFIGHGLANMQTRARSSGGDIDITSVQGEG
                     TTVFVWVPRGIKQ"
     gene            2052..3401
                     /gene="dnaA"
                     /locus_tag="PLR93_07290"
     CDS             2052..3401
                     /gene="dnaA"
                     /locus_tag="PLR93_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNH26806.1"
                     /translation="MNAEQAWQTVLAQLQMDMPRASYDTWVRDTRPVAYENGMLTVGV
                     RNAYARDWLDSRLATTVNKLLIDTLNSKVSVKFVVSQSEDNASIADLETAPASIEITP
                     PEPKPRHVTLNPRYTFDTYVVGSGNRLAHAACQAVAEKPARAYNPLFLYGGVGLGKTH
                     LLHAIGNACHASGLNVLYVSSEEFTNDMINAIRSHTTQAFREKYRSADVLLIDDIQFI
                     AGKESTQEEFFHTFNTLHGQDKQIIVSSDRPPKSLVTLEERLRSRFEWGLTADIQAPD
                     LETRLAILRSKAERTGRNISDEILESIAQRVQSNIRELEGALNRIIAYADLSGASLTP
                     NLVEVALADLMPSRADIEPAHVVDLVARKFGLTSEKLLGRDRTKEVALPRQIAMYLLR
                     EEAKISFPQIGEVLGGRDHSTVMSAYEKIKEQIHADRRLEQDIVSIKQQLYNQSVNA"
BASE COUNT          965 a          819 c          806 g          903 t
ORIGIN      
        1 cactgcatgt gaacaagccg gtgctcgtta tgctgcctta ggtgtattag acagtaaagg
       61 aaaactggaa aaatttattt ctgtagggtt gacacacgat gcgattaaac gcattgcaca
      121 cccaccggta ggacgaggtt tgttggggga gttaatggat acagaagacc cgttgcgggt
      181 gcggtctatt caagaacatc caaaatctgt tggtttccct gcacatcatc ctaaaatggt
      241 ttcattttta ggggttccga tccgcacggc agaagggcag atcggtcaga tttatttaac
      301 cgaaaaattg gatgcagatg aattctcgtc agatgacgag atgatcatcc aaatgcttgc
      361 cacatacgcc gcaacggcga ttgcgaatgc acgcctcatc gatcagatga aggaacgcga
      421 tgtagcgttg acgcgccgca acgtggatat gaccttgctg aacgacatcg cctccaccct
      481 gacctcaagc ctggaactgg acgagattct gaacaaaacg ctcgggcttg tgatgaacta
      541 catgaaagta gaagccggag aaatctttct tttagaggaa gataaaacca cactgcgcat
      601 gatgttacat cgtgggcaag ctgctgaagc cttctggacg cataatatct ttcgcgtagg
      661 ggagggatat cctggcgtag tggctgaaac ttgccgtccg ctcatttgta cagacctggc
      721 aaacgaccca aattttcaac gtgaagcggt agtgaaggcg ggatttcatc aagtattgag
      781 cttcccgctg gtttctggcg aacgggttat gggggttatg agcattgcta cccgttctaa
      841 agctgcattt gaagatagaa gtgtccaaat gctcacggct gtgggcgctt gggcagggct
      901 ttcgattgaa aatgcacgtt tacacgtcaa tgcacgtcgt ttagccgtgt tggaagaacg
      961 caatcgcatt ggtatggacc ttcacgatgg catcattcaa tccatttatg gggttggtct
     1021 tgcgctggaa ggtacaaaac tcctagtaga tgaagatgct tcatctgcaa aggagaaaat
     1081 tgataacgcc attgatggct taaaccaagc aattcgcgat ttacgagctt atattcttga
     1141 tctacgacca cgtcaacttg gtacagaagg cgtaattagt ggaattaaac gcttaattgc
     1201 tgaatatcgt gccaatacat tctctgaagt cacattcatg ccacctgatt cagacctcaa
     1261 agaactcact caaacgcaag ccttggcact ttttcatata tgtcaggaag cattagccaa
     1321 tgcagccaag catgccaaag caaaaaatgt tcaaatttca ctttggacaa cagaagatcg
     1381 tgctttgctt gagcttcatg atgatggaaa aggcttcaac accgatgaaa tgtcgacatt
     1441 tattggtcat ggtctcgcaa atatgcagac tcgtgcacgt tcatcaggtg gtgatattga
     1501 tataacatcg gttcaagggg aaggaacgac cgtatttgta tgggttccac gcggtatcaa
     1561 acaataaatc atgattgttg ccgaatcatt gccgggtgaa tcacaaaaac tcgtgtttca
     1621 cactttttga ttcagataag ttttgcatta tgattcttta agatagatat gccaagcctg
     1681 tgtcgaaaac cccttaaata cccatatatg ggggtcggtt cttggttcag gggcgttctt
     1741 ggctgaaacg cttgttctac aaaaaatgta gaaagaaagt aaacagataa caattcttta
     1801 acattgatga atcttaaaaa aaaccttttt tttaacttag cgtaggttga acgactgtta
     1861 catatatgaa tgttctgcta gaatatgacc acgctgatat aacggatgtt tgcccgtacg
     1921 attgacatcc gcaatcccta aaggatactt gacaaaacat ggatggaaat tcaagccgct
     1981 gttgggcggg gtggtttcat ctacgcaaaa aacaagcaag accataaaat tttgtacatt
     2041 gaggaacgtt tatgaacgct gagcaagcat ggcagaccgt ccttgcacaa ctccaaatgg
     2101 atatgccgcg tgcatcctat gatacgtggg tgcgcgatac ccgtcccgtt gcctatgaaa
     2161 acgggatgtt aaccgttggg gttcgcaatg cctatgcccg tgactggctc gatagccgcc
     2221 ttgccaccac tgttaacaag cttttgatcg acacgctgaa ctcaaaagtc tctgtgaaat
     2281 ttgttgtttc ccaatcggaa gataacgcct ctattgcgga cctcgaaact gctcctgctt
     2341 cgatagagat taccccgccg gaaccaaaac cccgccatgt aacgctcaat ccgcgctata
     2401 cattcgatac ctatgtggtc ggttcgggaa accgcctagc acatgcggct tgtcaggcag
     2461 tggcagagaa accagcacgt gcatacaacc cgctcttcct gtatggaggc gtgggactcg
     2521 gcaagaccca tctcttacat gccattggta atgcctgtca tgccagcgga ctcaacgtcc
     2581 tctatgtttc ttctgaagaa ttcacgaatg acatgatcaa tgccattcgt tcacatacca
     2641 cgcaagcctt tcgtgaaaaa taccgttcgg ctgatgtgtt gttgatcgat gatattcaat
     2701 ttatcgccgg gaaagaatcc actcaggaag agttcttcca caccttcaac accctgcacg
     2761 gacaagacaa acagatcatc gtttcgtctg atcgcccgcc caaatccctt gtgacgctgg
     2821 aagaacgctt gcgctcccgc tttgagtggg gcttaactgc cgatattcaa gcgccagacc
     2881 ttgaaacccg cctcgctatt ttgcgctcta aagccgagcg aaccgggcgt aatatctctg
     2941 atgaaattct cgaaagcatt gctcaacgcg tgcaatcgaa tatccgtgag cttgaagggg
     3001 ctctcaatcg catcattgcc tacgcagacc tgagcggagc atcccttact cccaatctcg
     3061 tcgaagttgc ccttgccgac ctcatgccct cccgtgcaga cattgaacct gcacacgtag
     3121 tggacttggt tgctcgcaaa tttggtctca cctctgaaaa attactcggg cgtgaccgca
     3181 ccaaagaagt ggcattacct cgtcagattg ctatgtatct cttgcgcgaa gaagctaaga
     3241 tctccttccc gcagattggc gaagtcctcg gcggacgcga ccactcgact gtcatgtctg
     3301 cttatgaaaa aatcaaagaa caaattcacg ccgaccgccg tttggaacaa gatattgttt
     3361 ctatcaaaca gcagttatat aaccaaagcg taaatgcata ataaatacag gaaccgaaat
     3421 gaacaggcgc acctaatgag gtgcgcttgt ttttatttta tggttaacca atgcattaat
     3481 cgcttttata cga
//
LOCUS       DAOQFT010000248         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15436_length_3486_cov_5.788691, whole genome shotgun sequence.
ACCESSION   DAOQFT010000248 DAOQFT010000000
VERSION     DAOQFT010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15436_length_3486_cov_5.788691"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..563)
                     /locus_tag="PLR93_07295"
     CDS             complement(60..563)
                     /locus_tag="PLR93_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH26807.1"
                     /translation="MDKKEIEFQLEDTQRRFIELVNSIPEENYSLPTDNPAWSVGDIL
                     FHITLGPRALALEIWMTAHASGFFGFMMRHFPSRLFNAINAWFGRGRSQRVSRQSLLK
                     AYGKAHAVIMSELRRTREEDLVKSVVYPPEFVSDLAGEVTPERLFRYVTGHFEAHEGQ
                     LRRDSLV"
     gene            822..1808
                     /locus_tag="PLR93_07300"
     CDS             822..1808
                     /locus_tag="PLR93_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH26808.1"
                     /translation="MSSVISISKLAKHYQVPEREAGLKAAAKGLFKRTYKTVKAVDGI
                     SFDIQPGEVVGFLGPNGAGKTTTLKMLSGLLHPTSGNLSVLGYEPAKRNHDYLRQITL
                     VMGNRNQLSWDLPALDSFDLQRAIYGIPTEEFKRTRDEFIELLDLKELVQKPVRNLSL
                     GERMKMEIVGALLHKPKILFLDEPTLGLDVTMQKRIRSFIAEYNKRYGATVLLTSHYM
                     ADVEALCKRVIVIHHGQLLFDGDLAALVQKFSAFKTVAFDIDNPNHDFSQYGDVVSID
                     GLHVTLRVPKEKTSAVTTRLLSDLAVDDLTVEATPIEDVIESVFSVKKEALE"
     gene            1805..2608
                     /locus_tag="PLR93_07305"
     CDS             1805..2608
                     /locus_tag="PLR93_07305"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNH26809.1"
                     /translation="MKRYFDFYFTSMKMAVIEQWQYPIANYFYMIGMIAEPVIYMVVW
                     SAVATQQGGTVGGYTPGAFAAYYIVWTLVRSMNVVFTPYGWEWRIRQGQLSMSLMRPI
                     HPLHNDVAFFAGWKVVVIILWIPLALALTLFFKPDLHPTLLNGIVFFFAIWFAYLIRT
                     MTLSLLGMVTFWTTRVSALFELYFAAELVLSGRLVPLSLMPEWVQKLAWFFPFRWAFG
                     YPIEALIGNMPVNDLFIGLGMQLVWILFGVMAVSLVWKVAIRRFSAVGG"
     gene            2610..3395
                     /locus_tag="PLR93_07310"
     CDS             2610..3395
                     /locus_tag="PLR93_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNH26810.1"
                     /translation="MTGIKLAFNYLRIGIMNEFQYRANLYIQILQTFIALGTGLIGLN
                     LVFAQTSQLGGWGKADLLAVMGVFTMTGGFIRAAIQPNMERLMDEIREGTLDYALTKP
                     ADSQLLVSVREFRLWQLVDVITGAIVLTVALLELKELINVWTVLGFFSALVMGAIMVY
                     SFWLIITSTAFWFVRVNEIANLFEGLYAAGRWPVDIYPNWLRYGLTFLVPVAFAVTVP
                     ASAITGRLTLQTWLLALAVTSILFILARTIWTIGVRNYSGASA"
BASE COUNT          786 a          873 c          829 g          998 t
ORIGIN      
        1 ttgcaggtct ccgtgccgaa agcttttaaa ccgtcggtag gaccagtcac ggggatggat
       61 tataccagag aatcccgccg taattgcccc tcatgcgcct cgaaatgccc tgtcacatac
      121 cgaaaaagac gctctggcgt gacctctcca gccaggtctg acacaaactc aggcggatac
      181 accaccgatt tgactaaatc ctcctcccgc gttcgcctca attcagacat tatgactgcg
      241 tgtgctttcc cgtaagcctt gagcaaactc tgacggctga ctcgttggct ccgtccgcgc
      301 ccgaaccagg cattgatcgc gttaaagagc cgtgaaggga aatgccgcat cataaagcca
      361 aagaatcctg aggcgtgtgc tgtcatccaa atttccagcg ccaatgcccg cggacccaag
      421 gtaatgtgaa agagaatatc tcccacactc caggctgggt tgtctgtagg gagagaatag
      481 ttttcttcag gtatcgaatt aaccagttca ataaatcgac gttgcgtatc ttctaattga
      541 aattcaatct cttttttatc cattttgctt tccatcttgc atttgatttt tggctgtgat
      601 acactttgct tatctaattc taatctgaaa ggaggtactt cttgaatgac tatcgttaat
      661 tatgaaacaa caaatctatg cgtgcctccc cgcgggtggg ttgggtaatt cattcccatt
      721 ttgaaacctt gccgcaggcg cgctgatgcc ctgcggtttt ttattgccat agggcggact
      781 attgataccg aacattaagc taccatattc aggagaaatc tatgtcgtct gtcatttcga
      841 tctcgaaact ggcaaaacat taccaagtcc ccgaacgtga agcgggttta aaagccgccg
      901 cgaaagggct cttcaaacgc acctacaaga ccgtcaaagc tgtagatggc atctcttttg
      961 atattcaacc cggagaagtg gtcggttttc tcggtccgaa tggggcagga aaaaccacca
     1021 cactcaaaat gctcagcgga cttttgcacc ccacatctgg gaatttatcg gtgttggggt
     1081 atgagcctgc taaacgtaac cacgattatt tacgccaaat cacgttagtg atgggaaacc
     1141 gcaatcaact ctcttgggat ttgcccgccc ttgattcatt tgatttgcaa cgtgccattt
     1201 atggcatccc aaccgaagag ttcaaacgca ctcgcgacga attcattgaa ttgttagatt
     1261 tgaaagaatt agtacagaag cccgttcgca atctctctct aggtgagcgc atgaagatgg
     1321 agattgtcgg cgcattacta cacaagccaa aaatcctctt cctcgatgag ccgacgctgg
     1381 gtctcgatgt gaccatgcag aagcgcatcc gttctttcat cgcggagtat aacaaacgct
     1441 acggcgcgac ggtgctgctc accagtcact acatggcgga tgtggaagcc ctgtgcaagc
     1501 gcgtcatcgt catccaccac ggacagttgc tttttgatgg tgatcttgcc gcgttagtcc
     1561 aaaaattctc cgcctttaaa acggtcgcct ttgatattga caatcccaac catgatttca
     1621 gtcaatatgg tgatgtggtc tccattgatg gcttacatgt gaccttgcgc gttcctaaag
     1681 agaaaacctc cgccgtcacg acccgtctgc ttagcgacct cgctgtagat gatctcactg
     1741 tcgaagccac gcctattgaa gatgtgatcg aatccgtctt ttcggtcaag aaagaggcgc
     1801 tggaatgaag cgatattttg acttctattt cacatccatg aagatggctg tcatcgaaca
     1861 gtggcagtat cccattgcca actatttcta tatgatcggt atgattgctg agccggttat
     1921 ttatatggtg gtctggtctg cggtggcaac ccagcaaggc ggcacagtgg gcggatatac
     1981 ccctggcgcg ttcgctgcct actacattgt gtggactctc gtccgtagca tgaatgtggt
     2041 tttcacgccc tacgggtggg aatggcgtat tcggcaaggt caactgtcga tgagtctgat
     2101 gcgccctatt catccgcttc acaacgatgt ggcattcttc gctggttgga aagtcgtcgt
     2161 cattatcttg tggattccgc tggcattggc gctcaccttg ttcttcaagc ctgacttgca
     2221 tccgaccttg ctaaatggca tcgtattctt ttttgcgatt tggtttgcgt acctgattcg
     2281 caccatgacc ctctctctgc ttggcatggt cactttttgg acgacacgtg tcagcgcctt
     2341 gttcgaattg tattttgccg ctgagttggt cctttcaggt cgccttgttc cgctttcttt
     2401 aatgccagag tgggtgcaaa aattagcatg gttcttccca ttccgttggg catttggtta
     2461 tcccattgag gcgctgatcg ggaatatgcc tgtcaatgac ctgttcattg gcttgggtat
     2521 gcagcttgta tggatcctat ttggtgtgat ggctgtaagc ctggtgtgga aagttgccat
     2581 ccgccgcttt tcggcagtag gaggttaata tgaccggaat aaaacttgcc ttcaactacc
     2641 tgcgaattgg catcatgaat gagtttcaat accgcgccaa tctttatatt cagattcttc
     2701 agacctttat cgccttgggc acggggttga ttggtttgaa tttggtcttc gcgcaaacgt
     2761 ctcaactcgg cggttggggc aaagcggacc tgttggctgt tatgggcgtt ttcactatga
     2821 caggcggatt tatccgcgct gccattcagc ctaatatgga gcgcctcatg gatgagatcc
     2881 gcgaagggac actcgactat gccctcacca aacctgccga ttcgcagtta ctcgtcagtg
     2941 tgcgcgagtt tcgcctttgg caattggtcg atgtgattac aggcgccatc gtgttgactg
     3001 tggcactctt agaactcaaa gagctgatta atgtatggac cgtgctaggc tttttctccg
     3061 cgcttgttat gggcgcgatc atggtttaca gtttttggct tatcatcaca tccactgctt
     3121 tttggtttgt gcgtgtaaat gaaattgcta accttttcga aggtctatat gccgccggac
     3181 gctggcccgt ggatatctac cctaactggc tgcgctacgg cttgaccttt ctcgtccctg
     3241 tcgcctttgc tgtgactgtg cccgcttccg ctattacggg acgattgacc ctgcaaacct
     3301 ggctccttgc tttggctgta accagcatcc tcttcatcct cgccagaacg atatggacta
     3361 tcggcgtacg gaactactcc ggcgcttctg cctaacaaaa aggatggtta ctttcaaagt
     3421 aaccatcctt tttacacatt agatttcata gtattatagc gggatgaaac atcgtatttt
     3481 tcaaaa
//
LOCUS       DAOQFT010000249         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15473_length_3482_cov_7.760432, whole genome shotgun sequence.
ACCESSION   DAOQFT010000249 DAOQFT010000000
VERSION     DAOQFT010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15473_length_3482_cov_7.760432"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="mnmA"
                     /locus_tag="PLR93_07315"
     CDS             complement(<1..802)
                     /gene="mnmA"
                     /locus_tag="PLR93_07315"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNH26811.1"
                     /translation="MTEKKKVVVAMSGGVDSSVAAALLKEQGYDVTGMMMRLWSEPGK
                     EESNRCCTPDSMAQARRVAGILDIPFYVVDAKDVFRETVVQYFLDGYANGETPNPCLM
                     CNRQIRWTFLLNHALALGAEFMATGHYVRIQNAEGGMRNLLRAVDHSKDQSYVLHVLG
                     QDKLQHALFPIGDYPKPEIRAIAERFGLPTASRKDSQDLCFLAGEDYRNFLQRNAPDM
                     LQPGNIETPDGNVIGEHNGLANYTIGQRKGLGVASAVPLYVITKNAKTN"
     gene            891..2480
                     /locus_tag="PLR93_07320"
     CDS             891..2480
                     /locus_tag="PLR93_07320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNH26812.1"
                     /translation="MSAKADIVIENAKVFTSNKEAPFAEAVAVKGNRIIYVGTNEGAK
                     ALKDSNTRVVDGQGRTLTPGFIDSHFHLLWGAISMGGAQLYDAKDINDVKTVLLEHAA
                     KNKTAEWVEGRGVRYDVIANRHELDAIIADKPIYINAYDGHTSWANTKALELAGILQP
                     GKEAEGVGLIVRDENGLATGELRETAMGLVSDLIPEPSEARKRELLKMTMANMNATGV
                     TSVHNMNGDMQELMTFAAVEDSGEMTVRVYEPYHIKPETTEEMLSEAAEMAKVKGEFV
                     RGGAAKFFMDGVWESYTALNVDPYADVPDAKPDGIYSLEHFTRMATLCDKMGMQIFVH
                     CCGDGAVRRTLDGYEAVQKANGKRDSRHRIEHIEVYHPDDLPRFKQLGVLPSMQTSHA
                     PFSIAEGDVWPTRVGKQRWHLSFAWRDIFNAGNQIAFGSDWPVAPYSPIINLHVGLNR
                     EPWAEGVSSHNLTLEELIIGYTRDAAYAEFMENEKGQIKEGYLADLVLFSHDLFALPK
                     ENIMETKPVLTMVNGKVVYEA"
     gene            2489..3463
                     /locus_tag="PLR93_07325"
     CDS             2489..3463
                     /locus_tag="PLR93_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH26813.1"
                     /translation="MGTYALRRILQTIPILFIISILLFLMVRAAPGGPLTAARRNPNI
                     SKEQIEAIEEKLGLNDPLPVQYGRWLGDMLRGDMGDSIKFRRPVSEMIGERVPNTLLL
                     VGASFLVTLLFAIPIGILSARKPYSKFDYAMTTVTFIGQAIPVYWLGLGLIVIFYVTI
                     KNPFTGNPLFPVGGMNTRGMEGNLLDTLWHLVLPVTALSLGWVAWYSRFLRSSMLDVL
                     HEDYVRTAHAKGLTDSLVYYKHALQNAILPLVTLIALDLPSLFAGALFVETIFSWPGM
                     GRLFWDAAKGRDYPVLLGVVMLTAVLIILCNIIADLAYGWLNPQVKYE"
BASE COUNT          859 a          897 c          877 g          849 t
ORIGIN      
        1 cattagtctt tgcattctta gtaatcacat aaagcgggac cgccgaagct acgcccagtc
       61 ccttacgctg accgatggtg taattcgcca agccattatg ttcaccgatc acgttaccat
      121 caggggtttc gatatttccg ggctgtagca tgtctggcgc attgcgttgt aaaaagttac
      181 ggtaatcttc gccagccaaa aagcacaagt cctgcgagtc tttgcgggaa gcggtcggca
      241 agccaaaccg ctccgcgatc gctcgaatct ccggcttggg gtaatctccg atcgggaaaa
      301 gcgcatgctg cagcttatcc tgccccaata catgcagcac atacgactga tctttagaat
      361 ggtcaacggc gcgaagtaaa ttccgcattc cgccttctgc attctgaatt ctcacataat
      421 gccctgtcgc catgaactct gcccccaacg ccagggcatg gttcagcaaa aacgtccaac
      481 ggatttggcg gttgcacatc aagcacggat tcggcgtttc gccattggca tagccgtcca
      541 gaaaatactg caccaccgtc tcgcggaaaa cgtccttggc atccaccaca taaaaaggaa
      601 tgtccaagat ccctgccacg cggcgcgcct gcgccatgga atcgggcgta cagcagcggt
      661 tggactcttc cttgccaggc tcactccaca ggcgcatcat catcccggtc acgtcatagc
      721 cctgctcctt gagcaaagct gccgcaaccg aggaatccac gccgccagac atggcgacaa
      781 caactttctt cttttcagtc attgagcaaa ttatacccag ttaaaatttc tcttgcccgc
      841 cggtcttaga taatgtatat tcaggtcaca ccactcaagg agattaagac atgagcgcta
      901 aagcagatat tgtcattgaa aatgcaaagg tctttaccag caacaaagaa gcaccgttcg
      961 cagaagccgt tgctgtaaaa ggaaatcgca tcatttatgt agggacaaac gaaggtgcca
     1021 aggcgctaaa agactcgaat acccgggtcg tggatggaca aggtcgcact ctgacgcccg
     1081 gcttcatcga ttcacatttt cacttacttt ggggtgccat ctcgatgggt ggagcgcagt
     1141 tgtatgatgc caaagatatc aacgacgtaa aaaccgttct attggaacat gccgccaaaa
     1201 ataaaactgc cgaatgggtt gaaggacgcg gcgtacgcta tgacgtgatc gcgaaccgcc
     1261 atgagttgga tgccatcatt gcagataaac caatttatat caatgcctat gacggtcaca
     1321 cgtcttgggc gaacaccaaa gccttggagt tggctggcat tctccagccg gggaaagagg
     1381 cggaaggcgt cggattgatt gtgcgcgatg agaacggtct cgctaccggt gaattgcgcg
     1441 agactgcaat ggggttggtc tccgacctga tccctgaacc gagcgaagcc cgcaaacgtg
     1501 aattgctcaa gatgacgatg gcaaatatga acgccaccgg cgtgaccagc gtccacaaca
     1561 tgaacggcga catgcaagag ttgatgactt tcgctgcggt agaagattcg ggggaaatga
     1621 ccgtccgcgt gtatgagccg tatcacatca agccggaaac caccgaagag atgctcagtg
     1681 aagccgctga gatggcaaag gtcaaaggcg agttcgtgcg cggcggtgcg gcaaaattct
     1741 ttatggatgg ggtctgggaa tcatacactg cactcaacgt tgacccttac gccgatgtgc
     1801 cagatgccaa acctgatggc atttattcgc tcgaacattt cacacgcatg gcaaccctat
     1861 gcgacaaaat gggcatgcag atctttgtcc attgttgcgg cgatggtgca gtgaggcgaa
     1921 cgctcgatgg gtacgaagcc gttcaaaaag cgaacgggaa gcgtgatagt cgtcaccgca
     1981 ttgagcacat tgaggtctat catcccgatg acctgccacg cttcaaacag ttgggcgtgt
     2041 tgccatccat gcaaaccagc catgctccct ttagcattgc cgagggagat gtatggccca
     2101 cacgggtagg caagcaacgt tggcatcttt cgtttgcctg gcgcgatatt tttaatgctg
     2161 gcaatcagat tgccttcggc agcgactggc ccgtagcgcc ctacagcccg ataatcaatt
     2221 tgcatgtcgg tttgaatcgt gaaccttggg cagaaggtgt gtcatcccac aacctgaccc
     2281 ttgaagaact tatcattggg tatacccgcg atgcggcata tgctgagttt atggaaaatg
     2341 aaaaaggtca aatcaaagaa gggtatcttg cagatttggt cttgttctcg catgacctat
     2401 ttgctttgcc caaagaaaac attatggaaa ccaagccggt cttgaccatg gtaaatggca
     2461 aggtcgtata cgaagcctag aggtactcat gggaacttac gccctgcgtc gcattttaca
     2521 aaccattccc attcttttca tcatcagtat tttattgttt ctgatggtgc gtgccgcgcc
     2581 gggcggaccg ttaacagccg ctcgcaggaa cccaaatatt tcgaaggaac agattgaagc
     2641 cattgaagaa aagctcggat taaatgatcc tttacctgtt caatatggac gttggctcgg
     2701 cgatatgctg cgcggtgata tgggcgactc gatcaagttt cgccgcccgg tttcagagat
     2761 gatcggtgaa cgcgtgccca atactctgtt attggtgggc gcctcttttc ttgtgacctt
     2821 gttatttgcc atccccattg gcattctctc cgctcgaaaa ccctactcca aattcgatta
     2881 cgccatgacg acggtcacct ttattgggca ggcaattcca gtttattggc tggggttggg
     2941 actcattgtg atcttttatg tcaccatcaa aaatcctttc acggggaatc cgcttttccc
     3001 agttggtgga atgaacaccc gtggcatgga aggaaatttg ctggacacgc tctggcattt
     3061 ggtcttgccc gttaccgccc taagtttagg atgggtcgct tggtattcac ggtttctacg
     3121 ctccagcatg ttagacgtgt tacacgaaga ttacgtccgc acggcacatg caaaagggtt
     3181 aacagatagt cttgtctatt acaaacacgc tttgcaaaat gcgattctgc cactggttac
     3241 cttgatcgcg cttgacttgc ccagtctttt tgcgggtgcg ttatttgtag aaaccatctt
     3301 ctcctggccc ggtatgggac gcctcttttg ggacgcagca aaggggcgag attaccccgt
     3361 cctccttggg gtcgtgatgc tcactgccgt tcttatcatc ctctgcaaca tcatcgcgga
     3421 tctggcttat ggctggctca acccacaggt gaaatatgaa tgagtcgagt ccgcttatca
     3481 aa
//
LOCUS       DAOQFT010000250         3479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15489_length_3479_cov_5.263143, whole genome shotgun sequence.
ACCESSION   DAOQFT010000250 DAOQFT010000000
VERSION     DAOQFT010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15489_length_3479_cov_5.263143"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2549)
                     /locus_tag="PLR93_07330"
     CDS             complement(<1..2549)
                     /locus_tag="PLR93_07330"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNH26814.1"
                     /translation="MSFNENTRVKIPAILHLSRLGYEYISLSKSKHDEDTNIFLDVFA
                     ESLLRINPGLDTESVKRVYQDVSLALENEDLGQAFYEMLTATSGTKLIDFENFDNNSF
                     HVVTELNYKNGDDEFRPDVILLINGMPLAFVEVKKPNNHDGILAERERINVRFKNKKF
                     KKFVNISQLLVFSNNMEYDTDSIEPIQGVFYSTTSYSNAIFHCFREEEELDLSALLKP
                     EDDDLENFVLKDNNLAIIKHSPEFLTNKDPNTPTNRMLTSLFSRERLAMLLKYGIAYV
                     KEGSGLEKHVMRYPQFFATKAIERKLGEGEKKGIIWHTQGSGKTALAFYNVRYLTDYF
                     HRKGIVPKFYFIVDRIDLMEQAKREFSSRSLSVNTVSSKDALLKSFRKKQAIENASGK
                     PEITVVNIQKFKDDTDTVKASDYDINTQRVYFLDEVHRSYDPKGSFLANLVSSDRDAI
                     IIGLRGTPLITGDNISRKVFGGYIHKYYYNASIADGYTLKLIREGIETEYKIQLEQAL
                     KEVEILKGDADKKTIYAHKKFAEPMLDYIVRDFVNSRTKYQDETIGGMVVCDSSEQAR
                     MLFKIFVNKYNPSQKTVEPVDMSYRKISEPPAEYGKFQQDTGRSFTASLILHDVGSKD
                     ERKEQVEDFKAGKIDFLFVYNMLLTGFDAKRLKKLYIARLLRDHNLLQMLTRVNRPYK
                     NFKYGFVVDFADIRAQFDKTNKAYFDELQAELGNEMENYSNLFKSSEEIEAEFKDIKE
                     KLFHYDTNNAEVFSQQISQIQDRKTMTDIRKALENARNLYNLARLFGHFDLLEKIDLR
                     KFNQLLDEVINHLELLNLKESIESDVDTTNLLNVALENVIFAFHKVSEDELV"
     gene            2734..2973
                     /locus_tag="PLR93_07335"
     CDS             2734..2973
                     /locus_tag="PLR93_07335"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNH26815.1"
                     /translation="MAISRIVQDINSLPPEAQKQVADFVAFLQSRYAPVPAKKMRRVK
                     LAREPFVGMWKDRADMKDSVEWVRQLRREQWRNKA"
     gene            2970..3362
                     /locus_tag="PLR93_07340"
     CDS             2970..3362
                     /locus_tag="PLR93_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH26816.1"
                     /translation="MSLTLVDTDILIDAALQVDEAIKCLDLLEETSSLAVSVVTQMEL
                     IIGCRNKSELRKLEQFLERFEIIPINERISNIATALLKAYHLSHGLLTADALIAATAI
                     DSDMDFITKNQRDYRFLEKLKLLSYPIK"
BASE COUNT          823 a          862 c          785 g         1009 t
ORIGIN      
        1 accagttcat cttctgaaac cttgtggaag gcaaagatca cattttccaa cgccacgttc
       61 agcaggttgg tcgtatccac atcgctttcg atgctttcct tcaaattcag caactccaga
      121 tgattgatca cctcgtccag cagttgattg aacttcctga gatcgatctt ctccagcagg
      181 tcgaaatgac cgaacagccg cgccagatta tataaattcc gcgcattctc cagcgccttg
      241 cggatgtcgg tcatcgtctt gcggtcttgg atctggctga tctgttgcga gaacacctcg
      301 gcattgttcg tgtcgtagtg aaagagtttt tccttgatgt ctttgaattc cgcttcgatc
      361 tcttcactgc tcttgaacaa gttggagtaa ttctccatct cgttgccgag ttccgcctgc
      421 agctcgtcga aatacgcctt gttggttttg tcaaattgtg cgcggatgtc ggcaaaatcc
      481 accacgaagc cgtacttgaa gtttttatac gggcggttca cgcgcgtcag catttgtaac
      541 aggttatggt cgcgcagcag gcgggcaatg tacagtttct tcaagcgttt ggcgtcgaag
      601 cctgttaaga gcatgttata cacgaacagg aaatcgatct ttcctgcttt gaaatcttcc
      661 acttgctcct tgcgctcatc tttcgagcca acgtcatgca ggatcaaaga cgcagtaaaa
      721 ctcctgcccg tgtcctgctg gaactttccg tattcggcgg gcggctcgga gatttttcgg
      781 tagctcatgt ccacaggttc caccgtcttc tggctggggt tgtacttgtt gacgaagatc
      841 ttgaacaaca tccgcgcctg ttccgaactg tcacacacca ccatcccgcc gatggtttca
      901 tcctgatatt tggtgcggct gttcacaaaa tcccgcacga tgtaatccag cattggctcg
      961 gcaaatttct tgtgggcgta gatggtcttc ttgtctgcgt cgcctttcag aatttcaacc
     1021 tctttgagcg cctgctccag ttggatcttg tactcggtct cgatcccttc acggatcaat
     1081 ttcaacgtgt acccgtccgc aatggaggcg ttgtaatagt atttgtgaat gtacccgcca
     1141 aacaccttgc gcgaaatatt atcacccgtg atcagcggcg tccccctcaa gccgatgatg
     1201 atcgcgtctc tgtccgaact gaccagattg gcaaggaaac ttccctttgg atcataactg
     1261 cggtgaactt catccagaaa atacacgcgc tgggtgttga tgtcataatc gctggctttc
     1321 accgtgtccg tgtcatcttt gaacttttgg atgttgacga cggttatctc aggcttgccg
     1381 ctggcatttt caatggcttg ctttttcctg aaacttttca acagcgcgtc tttggaactg
     1441 accgtattta cgctcaaact gcggctcgaa aactcgcgtt tggcttgttc catcaaatca
     1501 atgcggtcca cgatgaaata gaacttcggc acaatcccct tgcggtggaa gtagtctgtc
     1561 aggtagcgca cgttgtagaa agccaatgca gtttttccgc tgccctgcgt atgccagata
     1621 atgcccttct tttcgccttc gcccaatttg cgctcgatgg ctttggtggc aaagaattgc
     1681 ggatagcgca tcacgtgttt ttccagtcca ctgccttctt ttacataagc aatgccatac
     1741 ttaagcagca tcgccagccg ttcacggcta aagagcgagg tcaacatgcg gttggtgggc
     1801 gtgttggggt ctttattggt caaaaattct ggcgagtgct tgatgatggc aaggttgttg
     1861 tctttgagta caaaattctc aaggtcatca tcttcgggtt tcaagagcgc ggagaggtcg
     1921 agttcttcct cttccctgaa acaatgaaag atcgcgttcg agtaggaggt cgtcgaatag
     1981 aatacaccct gaatcggctc gatggaatcc gtatcatatt ccatgttgtt agagaaaacc
     2041 agaagttggg agatattgac gaattttttg aacttcttgt ttttgaaacg cacattgatt
     2101 cgttcccgtt ccgccagaat accatcatgg ttgtttggct tcttcacttc cacaaacgcc
     2161 aaaggcatac cattgatcaa cagaatcacg tcgggtcgga attcatcatc cccgtttttg
     2221 tagttcaatt ccgtgaccac atgaaaagag ttgttgtcaa aattctcaaa atcaatcagc
     2281 tttgtacctg aggtggcggt cagcatttca tagaacgcct gccccaaatc ctcattctcc
     2341 aacgccaacg aaacatcctg atagacacgt tttactgact ccgtatctag gccagggttg
     2401 atacgcagta gcgactcggc gaaaacgtct aggaagatat tggtatcttc atcgtgcttt
     2461 gatttggata atgaaatata ctcatatccc aacctgctca agtgtaaaat cgcggggatt
     2521 tttactctgg tgttctcgtt gaagctcatg tgcttgtccc ttttgtctct agaaacgttt
     2581 aactttcgca gggaattccc cgctgaattt ccccaagtat accccaccca aaatccacgt
     2641 ccacgggcgc tgacgcatct gcaaggcgtt ggctaattgt ggcctgtctc agtccgcttc
     2701 gcgaacaatg ctataatttg ttttgaggtg aatatggcaa tttcaaggat agtacaggat
     2761 atcaactctc ttcccccaga ggcgcagaag caggtagccg attttgtggc atttcttcaa
     2821 tcgcgctatg cccctgttcc tgcaaaaaag atgcgtcgtg taaaattggc gcgagagcct
     2881 tttgttggta tgtggaaaga ccgtgccgat atgaaagaca gtgtggaatg ggtgcgtcaa
     2941 ctccgccgtg agcagtggag aaacaaagca tgagtttgac acttgttgat actgatatct
     3001 tgattgatgc agctttgcag gttgatgagg caatcaaatg ccttgatctt cttgaagaaa
     3061 catcctccct ggctgtcagt gttgttactc aaatggagtt gattattggc tgccgtaata
     3121 aaagtgaact ccgtaaactt gagcagtttc ttgagaggtt tgaaataatt ccaattaacg
     3181 agagaatttc caatattgcg acggccttgt tgaaagccta tcatctgagc catggtttgt
     3241 taactgcaga tgccctaatc gcagctacgg ctattgactc agatatggat ttcatcacga
     3301 aaaaccagcg tgattatcgc tttttagaaa aactaaagct actctcatac cccataaagt
     3361 gaacaaacag attccccgcc taaaggggaa ttttttattc ctccctcatc cttcatcctt
     3421 tcctcctcac tcttcctccc acaccgaatg atacgccccc acggactcaa aatacttca
//
LOCUS       DAOQFT010000251         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15566_length_3467_cov_8.575029, whole genome shotgun sequence.
ACCESSION   DAOQFT010000251 DAOQFT010000000
VERSION     DAOQFT010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15566_length_3467_cov_8.575029"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..833
                     /locus_tag="PLR93_07345"
     CDS             36..833
                     /locus_tag="PLR93_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26817.1"
                     /translation="MELNFATITLPTIVVWMLGLFIFLLGSLLGYFNMSIDARKKQEL
                     AEQKVEHARIEADRRIAEMQKRLDEADIQPAKVQQTATTPSLLTLKSVNNLQTQIEMD
                     GQLLIAPLSPERKKRLVELINHFRPWLEGAASTSQPISKPLTTPLSEPLTNFKKPEAV
                     PYVPPTVKPVPVILGVNNQKTKTDPESEFKLLSMVKQIDVVLQKRLAGTSLESLGIHL
                     NDSLQGGLEVQIGSQKFETIDDVPDANIKAAIRAAIAEWEQKYIPGQ"
     gene            complement(888..1418)
                     /locus_tag="PLR93_07350"
     CDS             complement(888..1418)
                     /locus_tag="PLR93_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNH26818.1"
                     /translation="MSTDSQPKRYHPVHVTLHWLTALLVFMPLLSGFLILARTPDAQE
                     AQFLGMHKWIGIFLGLVIIARIITRFVFKRPAPADAGHPLLNWVGAIVHFLLYAGIFA
                     MLFSGDALDEAYGLEGILAGNGTMPESVMVYAEWARHSLFSYTLAALVALHVGAALYH
                     QFIRKDNLLSRMWYGK"
     gene            complement(1518..3329)
                     /gene="thrS"
                     /locus_tag="PLR93_07355"
     CDS             complement(1518..3329)
                     /gene="thrS"
                     /locus_tag="PLR93_07355"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNH26819.1"
                     /translation="MAQQVQEKYEESYLYKIRHSTAHVMAQAVLEIFPDGKPTIGPPV
                     ENGFYYDFDLPRNLTPEDLEAIEKRMRQIVQGKHDFKKTVISAEEAKKIFANQPYKLE
                     LIEGLEKGGLDEYGNPLKEKPEISIYQQDTFTDLCRGPHVANTKEIKPDAFKLMSVAG
                     AYWRGDENNKQLQRLYGTAWENKKQLEEYLHQLEEAKKRDHRKLGKDLEIFIFDEEVG
                     PGLPLWLPNGGIMIEELEKLAKEMEEKAGYDRVKTPSVTKEDLFLRSGHLPYYAESMF
                     PPMELEGVKYYVKPMNCPMHHKIFGSKGRSYRDLPVRLAEYGTCYRYEKSGELFGLMR
                     VRSMQMNDAHIYCSEEQFEQEFMGVVELYKKYFELFGIEKYVMRLSLHSKAGLGKKYV
                     DNPELWIKTEDMVRRAMDNGGVPYVEAEDEAAFYGPKIDVQIWSAIGREFSLATNQVD
                     FAVPSRFDLKFTNSEGHDEVPLCIHRAPLSTHERMVGFLLEHYAGNFPVWLSPEQVRV
                     ISITDEQNDYAEKIAKELRENGIRASADMSSQRMNAKIRNAQMMKVPYMIVVGKNEME
                     AGQISLRVRDGSQQNGFALSEFIARAKDRISRRASEL"
BASE COUNT          920 a          884 c          763 g          900 t
ORIGIN      
        1 tgcccaacct gccagccgct tgaaaggaaa taaccatgga gcttaatttc gccactatca
       61 ctcttcccac catcgttgta tggatgctag gactttttat ctttttactt ggcagcttac
      121 ttggctattt caatatgagc attgacgcac gcaagaaaca agaactggca gaacaaaagg
      181 tagaacatgc gcgtattgaa gcagatcgta gaattgctga aatgcaaaaa cggttagatg
      241 aagctgatat acagcctgcc aaggttcagc aaactgccac tactccttcg ttgctaacat
      301 taaaaagcgt aaataatcta caaactcaaa tcgaaatgga tgggcaacta ttaatcgccc
      361 ctctttctcc tgaacgaaaa aaacgtttag tggaacttat caatcatttt cgtccgtggc
      421 ttgaaggggc tgcatcaaca tcccagccta tttctaaacc tctcactacg cccctttctg
      481 aacctctaac taattttaag aagccggagg ctgttcctta tgtgccacct actgtaaagc
      541 cagttcctgt cattcttgga gtgaataatc aaaaaaccaa aacagaccct gagtctgagt
      601 tcaaattact aagcatggtt aagcagatcg acgttgtatt acaaaaaagg ttggcaggga
      661 cgtctctaga gtctctgggc attcatctca acgactcatt acaaggtggc ttggaagtgc
      721 agattggttc acaaaaattt gagacaattg atgatgtacc cgatgcaaat atcaaagctg
      781 ccatccgtgc agcaattgca gaatgggaac agaagtatat ccctggacaa taatttcgaa
      841 aaaagccctg ctcttgtgag cagggctttt ttactgttat atgcttttta cttcccgtac
      901 cacatacgag acaacaaatt atccttgcga ataaattgat gatataacgc cgcgcccaca
      961 tgcaaagcga ccaaagccgc cagagtgtac gaaaacagac tatgtcttgc ccattcggca
     1021 tagaccatca cactttctgg catggtcccg ttgcctgcta gaataccttc caaaccatag
     1081 gcttcatcaa gcgcatcgcc tgagaacagc atggcaaaaa tccctgcata tagcaagaag
     1141 tggacaatag cacccaccca gttcaaaagc ggatgcccag cgtctgctgg agccggacgc
     1201 ttgaatacga agcgggtaat aatacgggca ataatgacaa gaccgagaaa gataccgatc
     1261 catttatgca tgccgaggaa ttgtgcttct tgagcatccg gagtgcgggc aaggatcagg
     1321 aaacctgaaa gcaaaggcat aaagaccaaa agtgcggtca gccaatgcaa ggtaacgtgg
     1381 acgggatgat agcgtttggg ctgtgaatca gtagacataa tctctccttg gttttgattt
     1441 tcagataaaa ccccaaatcg ttttcagagt ttcaatagaa agacaacacc ggagatgtat
     1501 ttctccggtg ttgaaaatta cagttcactt gcgcggcggg agatgcgatc tttcgcccgt
     1561 gcaataaatt cgcttaaggc aaagccattt tgctggctac cgtcgcggac acgtaacgag
     1621 atctgcccgg cttccatttc attcttgcca accacaatca tatatggcac cttcatcatc
     1681 tgcgcattgc ggatcttggc attcatacgc tgggacgaca tgtctgcact ggcacggatg
     1741 ccgttctcgc gcaattcctt cgcgatcttt tcggcatagt cattctgctc atccgtaatc
     1801 gagatgacgc gcacctgttc cggcgagagc caaactggga agttgcccgc gtaatgctca
     1861 agcaggaagc ccaccatgcg ttcatgcgtg gacagcggcg cacgatgaat acaaagcggc
     1921 acttcatcgt gaccttcgct gttcgtgaac ttcaggtcaa agcgagaagg aaccgcaaag
     1981 tccacttgat tcgtagcaag cgagaactca cgtccaatcg cggaccaaat ctgcacatcg
     2041 atctttggac cgtagaatgc tgcttcgtct tcagcttcca cataaggaac gccaccattg
     2101 tccatggcgc ggcgcaccat gtcttcggtc ttgatccaca gctcagggtt gtctacatat
     2161 tttttgccaa gaccagcctt cgagtgaagc gagaggcgca tcacatactt ctcaataccg
     2221 aacaattcaa agtatttctt gtacagttcg actacaccca taaactcctg ctcgaactgt
     2281 tcttcggaac aatagatatg agcatcgttc atctgcatcg agcggacgcg catcagacca
     2341 aagagctcgc ctgatttttc gtagcgataa cacgtgccgt attcagcgag gcggacaggc
     2401 aagtcacggt aggagcggcc ctttgaaccg aagatcttgt gatgcatggg gcagttcatc
     2461 ggtttgacgt aatacttcac gccttccaat tccatgggcg gaaacatgct ctcagcataa
     2521 tagggcaagt gaccagagcg caggaacaga tcttccttcg tcacactcgg cgtcttgact
     2581 cggtcgtagc cagccttttc ttccatctct ttggcaagct tttcgagttc ttcgatcatg
     2641 atgccaccat tcggcaacca caaaggcaaa cctggtccca cctcttcgtc gaagatgaag
     2701 atttccaagt ctttgcccag tttgcgatgg tcgcgtttct tggcttcttc caactggtgc
     2761 aaatactctt ccagttgttt tttgttttcc caagccgtac catacaggcg ctgcaattgc
     2821 ttgttattct cgtcaccacg ccaataagcg ccagccacag acatcaactt gaatgcatcg
     2881 ggcttgatct ctttcgtatt tgccacgtgc ggaccacggc acaaatccgt gaacgtatct
     2941 tgctgataga tggatatttc cggcttctct ttaagcggat taccgtattc atccaagcca
     3001 cctttttcca agccttcgat caactccaac ttgtatggct ggttagcaaa gattttcttc
     3061 gcttcctctg ccgagatgac cgttttcttg aaatcatgtt tgccctgcac gatctggcgc
     3121 atgcgttttt cgatggcttc gaggtcttcc ggcgtcaggt tgcgtggcag atcgaaatcg
     3181 taataaaagc cattctccac gggcggacca atggtcggct tgccatcggg gaagatctcc
     3241 aacacggctt gtgccatcac atgtgccgtg gagtgacgga tcttataaag ataagactct
     3301 tcatattttt cttgaacttg ttgtgccatt ttcaattcct ttcgtaccag accatggact
     3361 atggacgatt gaccatggtc tatggtctgc ggtctatcgt ccaaataaaa aactctcatc
     3421 cacaacggga cgagagtttt aactcacgtg gttccacccg atttacc
//
LOCUS       DAOQFT010000252         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15607_length_3461_cov_7.252789, whole genome shotgun sequence.
ACCESSION   DAOQFT010000252 DAOQFT010000000
VERSION     DAOQFT010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15607_length_3461_cov_7.252789"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="PLR93_07360"
     CDS             complement(<1..1528)
                     /locus_tag="PLR93_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26820.1"
                     /translation="MKTPSSSTSTHHEDINDVIRRADILQRVMSTISSGLELDPLLSN
                     ILESAVKLIEATHGTIGLVGDRNGKPIIRTVATHNMPAEELGAEMAPGIGLAGKVLLE
                     KKTVRLDRYGSLEEPTLQGLAEHSVIGIPILWGEEMIGFFGIGVDARRFTDHDVETLE
                     YFAQYAAVAIHNANLFKANQHALEEMHLLYKTSQRIGLSDDVDGVIDAYLDQVAAGGK
                     YVCNICLYEFDENNERTEVIVKGRWMPETGSERLDEHLPYVQDNLDPILDAGETLIFS
                     DVRNDSRAPESLGEIQAASGRWALVMIPLMVRNQRIGLVILSHPGVHEWADEELSPYR
                     AVAAQLAIAIDHRSQQTLLQQRGQQLAVLQERQRLARELHDSVTQLIFSTTLIAQSIS
                     PAWKRNPEEGEKRVERLLELSQTALREMRSLLFELKSSEEIQDTELPVTLTGLERIRH
                     YGLLGAIRLLAQDFSADGILVSVDVDEASEKLFSKQTGSRSGKEPMVDESIYRITQEA
                     L"
     gene            1937..2962
                     /locus_tag="PLR93_07365"
     CDS             1937..2962
                     /locus_tag="PLR93_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNH26821.1"
                     /translation="MYICVLTSTPDDDDIPYDPSPYMNGYRWKQHHVEPTEVEKQIKQ
                     LMDDGVDVFVNLCDGTPDDALSGIALVKALEKYNAAFTGADSKFFDPTRDEMKNAAKR
                     VSVPTPSWAFASRAEDAEKIAKRLKFPMLVKPPHGYASVGIRRNSRVTTVDELREQLK
                     LAIAEFGRALIEEFVEGREFTCLIAENTKNPNKPFTFTPVEFIFPEGESFKHYDMKWV
                     DYEKMSVAVVSDPRIDKILREQTTRVFKELGGNGYARCDYRMDADGTIHMLEINPNCG
                     IFYPPHEPGSADFSLLNDPNTDHTKFMKLIISNAQHRQTRIAAERIIKRQQRKRERVP
                     VEQMAYT"
     gene            complement(2996..>3461)
                     /locus_tag="PLR93_07370"
     CDS             complement(2996..>3461)
                     /locus_tag="PLR93_07370"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26822.1"
                     /translation="EAARWFGVKALRDVSTQELGKWNPGSELSEIAFRRARHIVTENE
                     RVLEAIEVMRKGNIKRLGELFNASHNSLRDDFEVTNDALNIVVECAREQSSCYGARMT
                     GAGFGGCAVALVAEDKATEFVQAVSAVYKQRSGLEASIYVCKASEGASILQG"
BASE COUNT          959 a          865 c          710 g          927 t
ORIGIN      
        1 ttagcgcctc ttgagtgatg cggtaaatgc tttcatccac catcggctct ttccccgacc
       61 ttgaacctgt ctgcttacta aaaagctttt cactcgcctc atccacatcc acacttacaa
      121 gaataccatc tgctgaaaaa tcttgtgcca acaatcgaat ggcacctaat agcccataat
      181 ggcgaatacg ctccagcccg gtcaatgtga cgggcaactc agtatcttga atctcttcgc
      241 ttgatttcaa ttcaaacagt agcgaacgca tctcacgcaa cgcagtctgg ctcaattcca
      301 acaagcgttc tactcgcttt tcgccttcct ctgggttccg cttccacgcc ggcgaaattg
      361 actgagcaat gagagtcgtg ctaaagatca actgagtcac agagtcgtgc agttcacgag
      421 ccagccgctg acgttcctgt agcacagcca gttgctgtcc gcgttgttgt aacaaggtct
      481 gttgagagcg atggtcaatt gcaattgcca attgtgccgc cactgcccgg tatgggctta
      541 attcctcgtc agcccattca tgcaccccag ggtgactcaa gatcaccaat ccaatgcgtt
      601 gatttcgcac cattagcgga atcatcacca atgcccatct gccagatgct gcctgaattt
      661 caccaagcga ctctggcgct cgcgagtcat ttctgacatc tgagaagata agagtctctc
      721 ccgcatcaag aatggggtca aggttatctt gcacgtaggg caggtgttca tccaaacgtt
      781 ctgagccggt ttcgggcatc caacgtccct ttacaatgac ttctgtccgc tcgttgtttt
      841 catcgaattc ataaagacaa atattgcaga catatttacc gcccgccgca acttgatcca
      901 aataagcatc gatcactcca tctacatcat cggaaagtcc aatgcgttga cttgtcttat
      961 aaagcaggtg catttcttca agtgcatgct gatttgcctt gaaaagatta gcattgtgaa
     1021 tggcaacggc agcgtattga gcgaaatatt ccagagtttc cacatcatga tctgtaaaac
     1081 gccgagcatc tacgccaatt ccaaagaatc cgatcatctc ttcgccccat aaaatgggga
     1141 ttccaatgac ggaatgttct gcaagccctt gaagagtagg ttcttctagt gagccataac
     1201 ggtctaagcg tactgttttc ttttcaagta atacttttcc agctaaccct atccccggag
     1261 ccatttctgc gcctagttct tcggctggca tattatgagt tgccactgtg cggatgatag
     1321 gcttgccatt gcggtctcca acaagcccga tcgttccatg tgtagcttca attagtttga
     1381 cggcgctctc aaggatgttg gagagtagtg ggtcaagctc caatccgcta ctgatcgttg
     1441 acatcacacg ctgaagaata tccgcacggc ggattacatc attgatatct tcgtgatgtg
     1501 tagaggtgga tgaagaagga gttttcaagt ttacttgctc tgccaataag ttttacgaat
     1561 tatataccaa tgaatagatt gatttgaatg aaattgtaga ctgaaactta tatgcagagt
     1621 tggtgttaat tacacaagta aacatcaaac agagacgttc tttttcttaa ggacgttaat
     1681 aaccttaaaa accttaatga ctttatgaat aaacattata aaaagatgaa taaagctttc
     1741 aaatatttta agataaaacc atgtgtaaaa acctttacat aaacaaatag ttgtatacaa
     1801 taatcatata ttgatgataa tttaaatatt tttcatcata tgatgatatg taaaacgttt
     1861 agattgatag atagcatgcg acatgaagat aaataaaaaa tcatgaacgc aaaaaaggaa
     1921 tgacaaagga aatatcatgt atatctgcgt actaacaagc acacctgatg acgacgacat
     1981 cccatatgat ccttcaccat acatgaatgg atatcgttgg aagcagcatc atgtggaacc
     2041 aaccgaagtt gaaaaacaaa tcaaacaatt gatggatgat ggcgtggatg tattcgtcaa
     2101 tttgtgtgat ggtacacccg atgatgcact ttcaggaatt gccttggtca aggcgcttga
     2161 aaaatataat gctgccttta ctggtgcaga ttcgaaattt tttgacccca ctcgcgatga
     2221 aatgaagaac gccgccaagc gagtttctgt ccctacaccc agctgggcat ttgccagccg
     2281 tgcagaagat gcagagaaga ttgcgaagcg tctcaaattc cccatgcttg tcaagccgcc
     2341 tcatggctat gctagcgttg gcattcgccg caactcgcgc gtcactactg tcgatgagct
     2401 tcgtgaacaa ttgaaacttg ccatcgctga gtttggacgc gctcttattg aagaatttgt
     2461 ggaaggacgc gagtttacct gtctcatcgc agagaacacc aagaacccga acaagccttt
     2521 tacattcact ccggtagaat tcatcttccc cgaaggcgaa tctttcaaac actatgatat
     2581 gaaatgggtc gattacgaaa agatgtctgt agctgtggtg agcgatcccc gcatcgacaa
     2641 gatcttgcgt gaacaaacca ctcgggtctt caaagaattg ggtggcaacg gttatgctcg
     2701 ctgtgattat cgtatggatg cagatggcac tattcacatg ctcgagatca accccaactg
     2761 cggtatcttc tacccgcctc atgaaccggg ttctgccgat ttctccttgc tcaatgaccc
     2821 gaacaccgat cacaccaagt ttatgaagtt gatcattagc aatgcccagc atcgtcagac
     2881 tcgcattgcc gctgagcgca tcatcaagcg ccagcaacgc aagcgcgaac gcgtgccggt
     2941 cgaacaaatg gcttacacct aagaaaaacg ggacacttca aagtgtcccg tttttttatc
     3001 cttgaagaat acttgctcct tcactggctt tgcagacgta aatactcgcc tccaacccgg
     3061 acctttgctt gtataccgct gaaacagctt gaacaaactc tgtagctttg tcctctgcaa
     3121 ccaaagcaac cgcacagccg ccaaagcctg caccggtcat acgagcgcca taacaactgg
     3181 actgttctcg cgcacattca accacaatat tcagcgcatc gttcgtgacc tcaaaatcat
     3241 cgcgtagact gttatgactg gcgttaaata gttcacctaa gcgtttaata ttcccttttc
     3301 gcatcacttc gatggcttcc aacacacgct cattctcagt gacaatatgc cgggcacgcc
     3361 taaaggcaat ttcacttaac tcgcttccgg gattccactt tccaagctct tgtgtgctta
     3421 catctctcaa cgcttttacc ccgaaccagc gagccgcctc t
//
LOCUS       DAOQFT010000253         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15822_length_3438_cov_5.925805, whole genome shotgun sequence.
ACCESSION   DAOQFT010000253 DAOQFT010000000
VERSION     DAOQFT010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15822_length_3438_cov_5.925805"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLR93_07375"
     CDS             <1..186
                     /locus_tag="PLR93_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH26823.1"
                     /translation="QDPFSFGAYSHIPPFASGDDYDALAVPVDDVLFFAGEATSREYP
                     STVHGAYLSGLRAAEEM"
     gene            complement(232..1005)
                     /locus_tag="PLR93_07380"
     CDS             complement(232..1005)
                     /locus_tag="PLR93_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNH26824.1"
                     /translation="MDGFLGNQLGTYKVQTPVYEGPLDLLLSLIERAELDITTVSLAS
                     VTDQYLEHLRNVDQQKPDEISSFLVIATKLVQIKSEALLPRPPEREPGEEDPGEALVE
                     QLKLYKRYKEIAVWMEERQTKNLRTFLRVAPPPKVEPKLDLSNLTLEKLLAAAESAFA
                     KGKEKKPLGTVITAPKVTIREKIDYITKTLKNIQRMSFSGLITDKATRVEIVVTFLAL
                     LELVKRYRVTAKQDVLFGDIEFERSEDWKDDEEIEIEFE"
     gene            1041..1520
                     /gene="moaC"
                     /locus_tag="PLR93_07385"
     CDS             1041..1520
                     /gene="moaC"
                     /locus_tag="PLR93_07385"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNH26825.1"
                     /translation="MSKLTHLDEHGRAKMVDVSHKPDTTRTAVARGEVHMTKATFDLI
                     RAGQIKKGDVLTVAQIAGITASKRTSDLIPLCHPLPLSKVDVDLELDESLPGIVITAT
                     AKVTGKTGVEMEALTAVSVAALTVYDMAKAAEKTMHIQNIRLLEKHGGMSGDVINEP"
     gene            1531..1899
                     /locus_tag="PLR93_07390"
     CDS             1531..1899
                     /locus_tag="PLR93_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH26826.1"
                     /translation="MNYTSINLKEKLSKFSEHWSPKIIAQLNDYHLKLAKVQGEFVWH
                     DHPDTDEVFIVVQGHLDILFRDGKVSLDEGEMFVVPKGVEHKPVAEKECHILLIEPAG
                     LVNTGSAKDSELTAPNDVWI"
     gene            1901..2149
                     /gene="moaD"
                     /locus_tag="PLR93_07395"
     CDS             1901..2149
                     /gene="moaD"
                     /locus_tag="PLR93_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNH26827.1"
                     /translation="MNHIKILFFATIRDRAGTKSIELDIPADATILQLKEKLSADYPN
                     LKESLKSVLVTINREYVFDEAVVPTNAEIALFPPVSGG"
     gene            2159..2626
                     /locus_tag="PLR93_07400"
     CDS             2159..2626
                     /locus_tag="PLR93_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNH26828.1"
                     /translation="MQPSNHPTIYSITESEIDLNDLLAKITLTSTGAAAIFTGMVRGV
                     TTRGDAHETEYLEYESYVPMAEAKMQQVADEIREKWPVVEGIAIVQRIGKLYPKTPTV
                     LIACTAAHRDTGVFDAARYGIDRLKEIVPVWKKEVGPNGEEWVEGDYVPKPGE"
     gene            2636..3190
                     /locus_tag="PLR93_07405"
     CDS             2636..3190
                     /locus_tag="PLR93_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26829.1"
                     /translation="MHTQRLILRNFELADLDAYVGYRNDPEVGKYQGWVIPYSKEKGE
                     EFIREVQDMHAPKQGEWMQIAIALKETNVLIGDIGCVLRQEDARQAIIGYRIASPHWH
                     KGYAFEAVQAWLDFLFEDLNLHRVIADCDIENTASYKLLEKLGFRREAHFIESYLENG
                     GYTNEYHYGILQREWRAKAAFRNC"
     gene            3221..3421
                     /locus_tag="PLR93_07410"
     CDS             3221..3421
                     /locus_tag="PLR93_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26830.1"
                     /translation="MVTIRKAVLKDASAIATVHVAAWKEAYRGIVADEVLNNLSIQRR
                     TEQWTNSLSDEHTHITAHLWRR"
BASE COUNT          902 a          753 c          838 g          945 t
ORIGIN      
        1 caagacccgt tttcgtttgg tgcatattcg catattccgc cttttgcgag tggagatgat
       61 tatgatgcac ttgcggtacc agtagatgat gtgttgttct tcgcgggcga agcgacttca
      121 cgagagtatc ccagcacggt tcatggggca tatctctcag ggctacgagc tgcggaagag
      181 atgtaaaaaa taaaacggat gaggcaaaac ctcatccgct tttgattaat attactcaaa
      241 ctcaatttcg atttcttcgt cgtctttcca atcttcgctg cgttcgaatt caatatcgcc
      301 gaacaacaca tcttgcttgg ctgtgacgcg gtaacgcttg accaactcta gcaatgcaag
      361 aaaggtcact acaatttcaa cgcgagtggc tttgtcggta atcaatccac taaagctcat
      421 gcgctgaatg ttcttcaagg tcttggtgat gtaatcaatc ttttcgcgaa tggttacttt
      481 gggcgcagtg ataactgttc cgagcggttt cttctctttg cccttcgcaa acgctgattc
      541 cgctgccgct aacagcttct ctaaggtcaa attggaaagg tccagcttcg gttctacttt
      601 tggaggcgga gccacacgca agaacgtccg taagtttttt gtctggcgtt cttccatcca
      661 cacggcaatt tctttgtatc gcttgtaaag ttttaactgc tccacaagtg cttcgccagg
      721 gtcttcttca ccgggttcgc gctcaggtgg gcgtggcagc aacgcctccg atttgatttg
      781 caccaatttg gtggcaatca cgaggaaaga cgaaatctca tctggctttt gttggtctac
      841 attacgaaga tgttcaagat attggtctgt caccgaagcg agcgatacgg ttgtaatgtc
      901 taactcagcg cgctcgatta agctcaacaa aagatcaagc ggaccctcat acactggggt
      961 ctgaaccttg tacgtgccaa gttgattgcc gagaaatccg tccataagcc cgcgaattat
     1021 accagtgtat aatcttcgct atgtccaagc taacgcattt agatgaacat ggtcgtgcca
     1081 aaatggtgga tgtaagccac aagccagata cgacccgaac tgccgttgca cgcggtgagg
     1141 ttcatatgac caaggcgaca tttgacttaa tccgcgccgg gcagatcaaa aaaggcgatg
     1201 tgttgaccgt ggcgcaaatt gccggcatca ccgcttcaaa acggacctca gacctgatac
     1261 cgttatgtca tcctcttcca ctctccaaag tggatgtcga ccttgagttg gatgaatccc
     1321 ttcctggtat tgtcatcact gccaccgcca aagtgaccgg caaaaccggc gttgagatgg
     1381 aagccctaac ggctgtttcc gtcgctgcgt tgaccgtcta cgatatggca aaagctgccg
     1441 agaaaaccat gcacatccaa aatattcggt tgttggaaaa acatggtggt atgagcggcg
     1501 atgtgattaa tgaaccgtga tgggtgaacc atgaattaca cctctattaa tctcaaagaa
     1561 aaactttcga agtttagtga gcattggtcg ccgaagatta ttgctcaact taatgactat
     1621 catctgaagc tagccaaagt tcaaggtgaa tttgtctggc atgaccatcc tgatacagac
     1681 gaagtcttta ttgtggttca aggtcatctt gatattctgt ttcgtgatgg caaagtttca
     1741 ctggatgagg gtgagatgtt tgttgtgccc aaaggcgtgg agcataagcc tgttgctgaa
     1801 aaagaatgtc atattttatt gattgaacca gctgggctgg taaatactgg tagcgcaaaa
     1861 gactcggagt taaccgctcc aaatgatgta tggatataaa atgaatcaca taaaaatctt
     1921 attttttgcc actatccgtg accgtgcagg gacaaaatct attgagttgg atattcctgc
     1981 agatgcaacc atcttgcagt taaaggagaa gttgagcgca gattacccta acttgaaaga
     2041 gtcgcttaag tctgttttgg tcactataaa ccgcgaatat gtttttgatg aagcggtcgt
     2101 ccctacaaat gctgaaatcg cccttttccc tcccgtatct ggaggataat attcaactat
     2161 gcaaccttca aaccacccaa ccatttattc cattaccgag tctgaaattg acttgaacga
     2221 tttacttgcc aagatcacgc ttacatccac aggagcagcg gcgattttca cgggcatggt
     2281 acgtggcgtc acaacccgtg gcgatgctca cgagacggag tatctcgaat atgagtctta
     2341 tgtccctatg gcagaagcca agatgcagca agtagcagat gaaatccgcg agaaatggcc
     2401 cgtagtggag ggtattgcca ttgttcagcg cattggcaaa ctctacccga aaacgccgac
     2461 tgttcttatt gcttgtactg ctgcccatcg tgatacaggc gtttttgatg ctgcccgtta
     2521 tggtattgac cgcctcaagg aaattgtgcc tgtttggaag aaagaagtcg gacccaatgg
     2581 agaggaatgg gtcgaaggcg attacgttcc caagcccgga gaataagttt tgtttttgca
     2641 cacccaacga ttaatcctgc ggaattttga gcttgccgac cttgatgcct atgtaggcta
     2701 tcgaaacgac ccagaagtag gcaaatatca aggttgggtc attccttatt caaaagagaa
     2761 gggggaggaa tttattcgag aagtgcaaga tatgcatgct cctaagcaag gcgagtggat
     2821 gcagatcgcg attgctttga aagagactaa tgtattaata ggcgatatcg gttgcgtatt
     2881 gaggcaagaa gatgcccggc aggcaattat tggttatcgt attgcttccc ctcattggca
     2941 taaaggatat gcttttgaag ctgttcaagc ctggttggat tttttgtttg aagaccttaa
     3001 tttacatcgt gtcattgcag attgtgacat agaaaacaca gcctcatata aattgcttga
     3061 aaagctaggt tttcggcgcg aagctcattt tattgaaagc tatttagaga atgggggcta
     3121 taccaatgaa tatcattacg gcatccttca acgtgaatgg cgtgccaagg ctgcatttcg
     3181 taactgttga tttgttctga gccttaagaa gggagcaaag atggtcacta ttcgtaaggc
     3241 tgttttgaaa gatgcttctg cgattgctac tgtccatgtg gcggcgtgga aggaggcgta
     3301 ccgtggcatt gtggcggatg aggtgttgaa caatctgtcc attcagcggc gaaccgagca
     3361 gtggaccaat tctctttctg atgaacacac ccatatcacc gcgcatttgt ggcggagatg
     3421 aatagtcagg tcgtgggt
//
LOCUS       DAOQFT010000254         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_15954_length_3420_cov_5.856464, whole genome shotgun sequence.
ACCESSION   DAOQFT010000254 DAOQFT010000000
VERSION     DAOQFT010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15954_length_3420_cov_5.856464"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLR93_07415"
     CDS             complement(<1..410)
                     /locus_tag="PLR93_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH26831.1"
                     /translation="MTVTTYKLDASHLHLFNQFDRTFSVSSKVLLDMTGGRLTWGIIP
                     IEPYEKEIPIDEVDPATFINSEDKVVFLAEVDGKLAGQIKLITWWNGYAYIDDVIVNP
                     QFRGQGVGKALMEAAIQWSREKRFPGIMLETQDD"
     gene            complement(415..1410)
                     /locus_tag="PLR93_07420"
     CDS             complement(415..1410)
                     /locus_tag="PLR93_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNH26832.1"
                     /translation="MMKKPFLSSLLFLLFAIVLSLAIGSVFISPTELWKILRGVGEEK
                     FAFIIWNIRLPRTVLIALTGAALSGSGASYQGLFRNPLADPFLIGVASGAGLGAVIAM
                     SIQWPYSFWGLMAIPAAAFISALLTVFIVYFLARVGQTIPTTNLILAGVAFSSFATSL
                     TSFLMLRSTNEVRRALGWLLGGASQAGWTAILAMLPYLVIGLGILIFFGYQLNLLQFG
                     DDQAQQMGLNVNRTKTILLIASSLATAAAVAFSGIIGFIGLVVPHIMRLWFGADYRRL
                     IPLSIISGASALLFSDIIARIVLAPQEIPVGIITALAGAPFFLWVLRRVKNQGYW"
     gene            complement(1511..2482)
                     /locus_tag="PLR93_07425"
     CDS             complement(1511..2482)
                     /locus_tag="PLR93_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNH26833.1"
                     /translation="MFRKNLILTLLIALVAACAPAPQPTATVVPVSTEISVTDVVATE
                     AVAEGLSFTDGLGREVTLTAPAQRVVSLAPSNTEILFAVGAGDQVIGRDEFSDYPAEA
                     TSIESVGGSMGEYSAEAIVALEPDLVLAAEINTPELVSQLEDLGLTVYYLSNPTTIEE
                     MYTNLEIVGQLTGHDTTEMVDSLKARVAAVDEKIAPISSRPSVFYEIDASDPSKPWTY
                     GPGSFGNLLIERAGGFNIASDAPDSYPQLSLEQVVVANPSVILLGDSMWGVTPESVLD
                     REGWNTILAVQNNQIFPIDDNLISRPGPRLVDGLEALAKILHPGLFE"
     regulatory      complement(2564..2718)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            complement(2718..>3420)
                     /locus_tag="PLR93_07430"
     CDS             complement(2718..>3420)
                     /locus_tag="PLR93_07430"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HNH26834.1"
                     /translation="TELMKMYANARGHKRSLFLLPYVPVWFMAFGIDLMTPVPYPIAY
                     ALVGGLSSDSIILHANAEKAFPEVKLMDYESAIVEAMKRLHPLKIERVWDDGSSGVKI
                     LKHEGFFIDHRAVDVNLPAEKIMNVIKGFDKDGEWLFANWLWKLRGAIEGKRSDFYEV
                     DAQSENLILFRSKLKSPGEGWMEWRVEDNLLIQTAYFAPRGLGGFLYWYLLAPFHAYV
                     FQGLLKSIIQKAMVQ"
BASE COUNT          926 a          812 c          887 g          795 t
ORIGIN      
        1 ttatcatctt gagtttcgag catgatgccg ggaaaacgct tctctcttga ccattgaatg
       61 gcagcctcca tcagggcttt gcctacgcct tgacctctga actgcgggtt gacgatcaca
      121 tcatcaatat aggcatatcc gttccaccat gtgatgagct tgatttgccc ggcaagtttg
      181 ccatccactt ccgcaaggaa gaccacttta tcttcactgt tgataaaagt tgcagggtca
      241 acctcatcga tggggatttc tttttcatat ggttctatag gtatgatgcc ccaggttaat
      301 ctgccaccgg tcatatctag taagaccttt gatgagacgg aaaaggttcg atcaaattgg
      361 ttgaaaagat gcaggtgaga tgcgtcgagt ttgtatgtag tgactgtcat gtctttacca
      421 atacccttga ttttttacac ggcggagaac ccacaaaaag aaaggtgcgc cagccaatgc
      481 agtgatgatg cctaccggaa tctcttgcgg agcaagcaca atgcgggcga tgatatctga
      541 aaataaaagc gcagatgcgc cgctgataat agagagcgga atgagtcttc gataatctgc
      601 gccgaaccac aggcgcataa tatgtggcac caccagaccg ataaagccga tgatgccaga
      661 gaatgccact gctgctgcgg ttgcaagcga cgatgcgatt agcaaaatag tttttgtgcg
      721 gttgacgttc agccccattt gctgagcttg gtcatcgccg aattgcaaaa ggttgagttg
      781 gtacccgaag aaaatcagaa tccccagacc aatgacaaga tatggcagca tggcgaggat
      841 agccgtccac ccggcttgac tagctcctcc aagcaaccat cccagtgcgc ggcgaacttc
      901 gttggtggag cgcagcatta gaaaggaggt gagtgatgta gcgaaagaag agaaggcaac
      961 cccggctaga atcaaatttg tggtgggaat ggtttgacca acccgggcaa gaaaataaac
     1021 aataaatact gtaagcaatg ccgagataaa tgcagcggca ggaattgcca tgagtcccca
     1081 aaacgaatac ggccactgaa tagacatggc aatcaccgcc cccagccctg cgccagaggc
     1141 cacgccaatc aagaatgggt ctgcgagcgg gttacgaaat aacccttggt atgatgcgcc
     1201 gcttccgctt agggcggcac cggtaagagc gattagcact gtgcgtggta agcggatgtt
     1261 ccaaatgatg aaggcaaatt tttcttctcc aacaccgcgc agaatcttcc ataactccgt
     1321 tggggaaatg aaaaccgagc caatggcgag actgagcaca atggcgaata aaaggaaaag
     1381 caaagaagaa agaaaaggtt ttttcatcat aacgagtgca tcgcaccaaa ggataggata
     1441 agtttaccgt gtagttttgc tgatgtaagg agacttatct acccatttgg tgcgacacac
     1501 gtttggtcaa ttactcaaat agaccggggt gcaaaatctt tgccaatgct tcaagcccat
     1561 ctaccaggcg cggaccgggg cggctgatga ggttatcgtc gatggggaag atctggttgt
     1621 tttgaacggc aaggatagtg ttccaacctt cgcgatcgag cacggattca ggcgtaacac
     1681 cccacatgga atctccaagc aaaatgacgg aaggattcgc gacgacaact tgttccaaac
     1741 ttaattgggg ataggaatca ggcgcatcgc tggcaatgtt gaacccgccg gcacgttcga
     1801 tcaataaatt gccgaaggaa ccgggaccat acgtccaagg cttggagggg tcagaggcat
     1861 caatttcata gaatacggat gggcgtgagc taataggcgc gattttctca tccacagcgg
     1921 caacgcgagc cttgagcgaa tcgaccattt cggtggtgtc gtgtccagta agttgaccga
     1981 cgatctcaag attggtgtac atttcttcga tcgtagtggg gttgctcaaa taataaacgg
     2041 tgagccccaa gtcttctaac tggctgacga gttcaggcgt gttgatctca gcggcgagca
     2101 ccagatcggg ttcgagggca acgatggctt cggcactata ttcgcccatg gaaccgccta
     2161 cactttcgat ggaagtggct tcagcagggt aatcggaaaa ttcgtcgcga ccaatgactt
     2221 ggtcaccggc gccgacggca aagaggattt ctgtgttgga aggtgcaagt gaaacgacgc
     2281 gctgggcagg cgcggtaaga gtcacttcgc gacccaaacc atcggtgaag gataagcctt
     2341 cagcaacagc ttcggtggcg acgacatcag tgacagaaat ttctgtcgaa acgggaacaa
     2401 ctgtcgctgt aggttgaggc gcaggggcac atgcggcaac gagcgcgatc agtaaagtca
     2461 aaatcaaatt cttacgaaac atcttttctc ctattgtgtg tatggaatca aaatccccag
     2521 cataggctgg ggaggtggga gagtcaatcc gggctggact tgttctccac ctcctttccg
     2581 cgagaagtgg ttgaaccgaa tcagagcggg cgacctggct tggtagggtc acgttttacg
     2641 cgtgacctac ttcacagttg cgcgacagcg ccgggtttac accggacttc gccgctggct
     2701 gattcgttat attgaaatca ttgtaccatt gctttttgga ttatggactt taacaaacct
     2761 tgaaacacat aggcatgaaa tggcgcaagc aaataccaat ataaaaatcc gccaagcccg
     2821 cgtggagcaa agtatgctgt ttgaatgaga aggttatctt ccacacgcca ttccatccaa
     2881 ccttcgccgg gcgattttag tttggagcgg aacaaaataa gattctcgct ttgagcatcc
     2941 acttcataaa aatcactgcg ctttccttca atcgcgccgc gcaatttcca taaccaattg
     3001 gcaaagagcc actctccatc tttatcgaag cccttgatga cattcatgat tttttcagca
     3061 gggagattta catctacagc acgatgatca atgaaaaaac cttcgtgctt caggattttg
     3121 actccgcttg agccgtcgtc ccatacccgc tctattttca atggatgcaa acgcttcatg
     3181 gcttcgacga tagctgattc gtaatccatg agctttacct cagggaaggc tttctctgcg
     3241 ttcgcatgca aaatgatgct atcgctcgaa agcccaccga cgagtgcata cgcgatcggg
     3301 taggggacgg gagtcatcaa gtcgatgccg aatgccatga accagaccgg cacataggga
     3361 agcaggaaca agctgcgttt atgtccgcga gcgttggcgt acattttcat caactcggtg
//
LOCUS       DAOQFT010000255         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16075_length_3405_cov_5.454925, whole genome shotgun sequence.
ACCESSION   DAOQFT010000255 DAOQFT010000000
VERSION     DAOQFT010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16075_length_3405_cov_5.454925"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..2411
                     /locus_tag="PLR93_07435"
     CDS             69..2411
                     /locus_tag="PLR93_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH26835.1"
                     /translation="MSDFIQLALNQLTVPPGDMIYYIVLAFTIASALQSAFNHWRASE
                     FPQARRAFIGLGILLSAQIFMFLFSGLGWQELIDSKAVLPPMDRAFLVFGIVWISWLY
                     AFPEPNRQADAVATLLSMFIMTALGLSLISWQQQMANPATATLSYNLTLDDFIWQIGS
                     VLLALLGMAVLFIRKPDGMWYGLILLFLGFLGHVGQLLIGLEGDFSGIVRLAYITAFP
                     ILLTLPQRFGTVTVVETSAPVKPTILKQDTAGRPERRRYSTDPKTFHALLAVAAENNS
                     TKMSQAITRAIAQTMLADLCFMIYLTDNNNQMVIAGGYDLIREDTLEGGSLSKNAIPM
                     LANSIQRGRPLRLPASSTSADIKGLGDILGLTNPGHLLYVPILTPEKETLGGILLLSP
                     YSDRTWSAEDQAFLSNIASSLVPIIQRNQKITKLEAQSDMARAQVADLEERIRNLNGQ
                     LDAARTEVERNKSTDVASLRVAQEESQNIIEQLQRENAELRSGKNLPSAQAEKELKAT
                     LQEMAILQNQLADANIKLLELEKGKIATKSTEQAEVLASISQELRQPLSSIVGYTDLL
                     LGESVGILGALQRKFVDRIKVSTERIRSLTDDMIQITTLATDLYSLNPEAVDLNSIID
                     NAMSYTSSQIREKNISIHLEVPKAVTSIHADREALQQIVIHLLQNAGAATPFEGTIRL
                     KVQTQNEDGAEYILIQVADQGGGIASEDIPRVFTRLYRADNVLIQGVGDTGVGLSIAK
                     TLTEAQHGRIWVESKVGEGSTFSVLLPISGDKLADKQKRK"
     gene            2415..3224
                     /locus_tag="PLR93_07440"
     CDS             2415..3224
                     /locus_tag="PLR93_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH26836.1"
                     /translation="MRGVRDFGNALVAAILSIGLTLGALSISLVGFIPEEVATPSPTL
                     IVSPIPVTATNTLPPTATLLVTVNIPTSSPTNTIVPPTSCQPPVGWVGVVVQVGDTLD
                     SLAARYGTYKDILKNKNCLFTDSLIAGTLLYVPGGISTSTVVACNQGAVGWIKNYMVR
                     PGDTFYNIGTRYGVNANLIKLVNCRTSDIIYAGEVLYVPNVPTRTPTFTPLPGITYTA
                     APLLTEPLTLTVLPFTATYPPTQTSTATTAPTASPIPTQTASLTAIPTPTP"
BASE COUNT          868 a          925 c          727 g          885 t
ORIGIN      
        1 gctctttgta gcagcataat ttcaattcta cgccataatc cctaactggc agtataatca
       61 cgagcggaat gagcgatttt atacagttag cattaaacca actcactgtt ccaccgggag
      121 atatgatcta ctacatcgtt ctggcgttta ccatcgccag cgcgttgcaa tctgcattta
      181 atcattggcg ggcaagtgaa tttccacaag cacggcgtgc tttcattggt ctgggcattt
      241 tactcagcgc acaaatcttt atgtttctct tcagcgggct gggctggcaa gaattaatcg
      301 actcgaaagc cgtcttacca cccatggacc gtgccttttt ggttttcggc atcgtgtgga
      361 tctcttggtt atacgctttc cccgaaccca atcgccaagc tgatgcagtt gccacccttc
      421 tcagcatgtt cattatgact gccttggggc tgagtttaat ctcatggcaa cagcaaatgg
      481 caaaccctgc cactgccaca ttgagctata acttaaccct agacgatttt atctggcaaa
      541 ttggttctgt actgctcgcc ttgttgggca tggctgtgct tttcatccgc aaaccggatg
      601 gaatgtggta cggattgatc ttgttgtttc tcggcttttt agggcatgtc ggtcaattat
      661 tgattgggct ggaaggcgat ttctctggaa tcgtccgctt ggcgtatata acggctttcc
      721 ccattttatt gacacttcca cagcgtttcg gcacagtgac cgtggtcgaa acgtcggctc
      781 cggtcaaacc gaccattttg aaacaagata cagcgggtcg ccctgaacgg aggcgttaca
      841 gcacagaccc gaaaacattc catgccttgt tggcagttgc cgctgagaac aactccacca
      901 agatgagtca ggcaatcaca cgtgccattg cacaaaccat gcttgccgac ttgtgcttta
      961 tgatctacct gaccgataac aacaaccaaa tggtgatcgc cggcgggtat gacttgatcc
     1021 gcgaagacac attggaaggc ggctcgctat cgaagaatgc cattccaatg ctggcgaact
     1081 ccatccaacg cggacgtcct ttacgtttac ctgccagcag cacctcagca gatatcaaag
     1141 gcttgggcga tattcttggg ctcaccaacc ctgggcattt gttatatgtc cccatcctca
     1201 ccccagaaaa agaaaccttg ggcggtattc ttctcttatc tccatactca gaccggacct
     1261 ggagtgctga agatcaggct ttcctttcta atattgcttc ttctttggta ccgattattc
     1321 aacgtaacca aaagattacc aagctcgaag ctcaaagcga tatggctcgc gcccaagttg
     1381 cagatttgga agaacgcatt cgcaatttga atggtcaact tgatgcagct cgtacagaag
     1441 ttgaacgtaa taaatcaaca gacgtcgctt cattacgtgt agcgcaggaa gaatcccaga
     1501 acatcattga gcagttacag cgcgaaaatg cagaattacg ctctgggaag aacctgcctt
     1561 ctgctcaagc agaaaaagaa ttaaaagcaa ccttgcaaga aatggcaatt ttacagaacc
     1621 agttggcaga tgctaatatc aagcttttag aattggaaaa agggaaaatc gctaccaaga
     1681 gcaccgagca agcagaagtg ctcgcttcca tttctcagga acttcgtcag ccgctttctt
     1741 ctattgtcgg gtatacagac ctgttgctgg gcgagtcagt cggtattctc ggtgcgttac
     1801 agcgcaaatt tgtggaccgc atcaaggttt ccacagaacg tatccgcagc ctcacagacg
     1861 atatgatcca aatcacaacg ctggctaccg acctgtattc gctcaatcct gaagctgtag
     1921 acttgaactc aatcatcgac aatgccatgt cgtacaccag ctctcagatt cgtgagaaaa
     1981 atatttccat tcatctggaa gttccaaagg cagtcacatc catccacgcg gacagggaag
     2041 ccctacaaca aattgtcatt cacttgttgc aaaacgccgg agcggcaact cccttcgaag
     2101 ggacgatccg cctcaaagtc cagacccaga acgaagatgg cgctgagtac attttgattc
     2161 aagtcgcaga tcaaggcggc gggattgcct ccgaagatat tccgcgcgtt tttacccgtc
     2221 tctatcgagc tgataatgtt ctcattcaag gggttggtga taccggtgta ggtctttcca
     2281 ttgccaaaac cctcaccgaa gcccagcatg gtcgtatttg ggttgaaagc aaagtgggag
     2341 aaggctccac gtttagtgtg cttttgccaa tctctggcga caaacttgcc gacaagcaaa
     2401 aaaggaaata aacaatgcga ggcgtgcgcg attttggaaa cgcgcttgtt gcggcaattc
     2461 tctcaattgg gttaacactg ggagcgttgt caatttctct tgttggcttt atccctgaag
     2521 aagtcgccac tccatcccca acactgattg tttctcctat tccggtaact gctacaaaca
     2581 ccttgccgcc aacggctact ttgttagtca ccgttaatat tcctacatct tcaccaacca
     2641 ataccattgt tcctccaacc tcctgtcaac cacctgtggg ctgggtggga gttgttgttc
     2701 aagtaggcga cacgttagat agcctcgcag caaggtatgg cacgtataaa gacattctca
     2761 aaaacaaaaa ctgccttttc acagacagtc ttattgccgg cacgcttcta tatgtgccgg
     2821 gtggcatttc caccagtaca gtagttgctt gtaaccaagg cgccgtcggt tggattaaaa
     2881 actatatggt tcgcccaggc gatactttct ataatattgg cactcgctat ggcgtcaacg
     2941 ccaacttaat caaactcgtc aactgccgaa ctagcgacat tatttatgcg ggtgaagttc
     3001 tttatgtacc aaacgtaccc acacgcacac caacctttac acctttgcct ggtattacct
     3061 acaccgcggc tccattactc accgaaccat taaccctaac ggttctgcct ttcacggcaa
     3121 catacccgcc aacccaaaca tccactgcca ccaccgcacc tacggcttcg cccattccaa
     3181 cccaaaccgc cagcttaacc gctatcccca ccccaacgcc ctaagcaggc gcactttcca
     3241 taacacatcg atgaaatcat ccaacctttt tgtatgggcg ctttgctttg ccgtttttat
     3301 ttcagcttgt ggtgcagcgg ctccgaccca agcgcctcag ccttttgttt taatcactgc
     3361 cgctccaaac gcatcgccta caccaacccc ctttcaacct tcctt
//
LOCUS       DAOQFT010000256         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16105_length_3401_cov_6.087866, whole genome shotgun sequence.
ACCESSION   DAOQFT010000256 DAOQFT010000000
VERSION     DAOQFT010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16105_length_3401_cov_6.087866"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLR93_07445"
     CDS             <1..380
                     /locus_tag="PLR93_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HNH26837.1"
                     /translation="YNQSAKDIQLANAIARAVKSISVDLILVGLAGSRSIEAGRAMGL
                     RVAAEGFPDRGYNADGSLMSRLLPGALIESPEDVARHAIELVKTGKMDTLCLHGDHPN
                     AAENAKKLREVLEKNRVEVVGLT"
     gene            complement(479..1798)
                     /locus_tag="PLR93_07450"
     CDS             complement(479..1798)
                     /locus_tag="PLR93_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26838.1"
                     /translation="MSADYAKELLRSGITDAKTGSKESARRYLDRAIYMAGSHDVLAE
                     AWYWMSEVVDDPAEKRKALENCLSHDLHHTRARRSLAILDGKLKAGDVINPDKLPAAP
                     EGLREAQADRFMCPKCGGRMAFSPDGSGLVCDYCTRQNPLGAGSGTENEKDFIVAMAT
                     MKGHGKPLQEQVFHCNGCGAEFILPPNQISSNCAYCDSPHVVSYEKTNDIIAPNSILP
                     FAIDQKRATHLLVEWVEKNHIKPEKKVDLPRGVYLPMWTFDLGGEIDYSGEIVEYEQN
                     LFTGERERKVTRVEDKYPVMVNDLALPASRKLSQVFMKLLPTFDLAALKEYDPRYLAS
                     WLAELYDVPMADASLDARSQAFKKLKKQLPHLLGNITLTRASSASMLVESFKLVLLPV
                     WMTELPFDGREHIVLINGQSGVVASDLSEKDEEHGGLFDFLADLLND"
     gene            complement(1788..2678)
                     /gene="folP"
                     /locus_tag="PLR93_07455"
     CDS             complement(1788..2678)
                     /gene="folP"
                     /locus_tag="PLR93_07455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNH26839.1"
                     /translation="MKSTTLQVGSFTFDWGSRTYVMGILNVTPDSFSGDGIIAKGDVV
                     KAAVEQAREFILYGADILDVGGESTRPGSEPVNAEEEMERVIPVIEELRKNFPQVLIS
                     VDTYKAKVAEAAIQAGANIINDVWGLRADSELASVAAKHKVPVILMHNRSNPASVEVR
                     EKLGGTYLGAEYDDLLEDVKRELLASVEIAVKAGVEEKLIMLDPGIGFGKKREHNLEL
                     INRLAEIRSLGYPILLGTSRKSFIGFTLDLPPDQRVEGTAATVAVGITRGADIIRVHD
                     VKEMVRVAKMTDAMVRVNER"
     gene            complement(2732..3154)
                     /locus_tag="PLR93_07460"
     CDS             complement(2732..3154)
                     /locus_tag="PLR93_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26840.1"
                     /translation="MSDPKPLNWTDTPGIGYSVVRRGDGGMTLTFTDLSDATMQHWHQ
                     FALDHLLGADRRTRNLYDLRSVPEIPEKAIRMAVDANSDPSARNIRVAVVVASEAVAD
                     GIRKVAALAETESAAAMKIFTDINEAEAWLARPLDQIA"
     gene            3249..3322
                     /locus_tag="PLR93_07465"
     tRNA            3249..3322
                     /locus_tag="PLR93_07465"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3283..3285,aa:Val,seq:tac)
BASE COUNT          832 a          871 c          837 g          861 t
ORIGIN      
        1 tgtataacca atcggcaaag gacattcaac tggcaaatgc aatcgcacga gcggtgaaaa
       61 gcatcagtgt agatttaatt ttggttgggc tggcaggctc aaggtcgatt gaggcagggc
      121 gggcgatggg gctgagagtt gcagcggagg gtttccccga tagaggctac aacgctgacg
      181 ggtcgttgat gtcccgcctt cttccggggg cattgatcga gtcaccggag gatgtggcaa
      241 gacatgcgat tgagttagtg aaaaccggga agatggatac gctctgcttg catggcgacc
      301 acccaaatgc ggcagagaat gcgaagaagc tgagagaagt tttagagaaa aatagggttg
      361 aggttgtggg attaacgtag gggcgaccca gatacattta atgaaaactt tttgcaaaag
      421 gcgaccctta ctttgaactt tgatttcagc tcaaatcttg acggggcgcc cctacaaatt
      481 aatcattcaa aaggtctgcc agaaaatcga acaagccgcc atgttcttca tctttttcag
      541 ataaatcact ggcaactacg ccgctctgac cattgatgag cacaatatgt tcgcgtccgt
      601 caaaaggaag ttcggtcatc cacactggta ggagaaccaa cttgaaggac tcgaccaaca
      661 tacttgccga tgatgcgcgt gtaagggtga tgttgccaag caaatgcggc aattgcttct
      721 tgagtttttt aaatgcttga ctgcgcgcat ccagtgaggc atctgccatg gggacatcat
      781 aaagctcagc caaccagctt gcgagataac ggggatcata ctctttgagt gcggcaaggt
      841 caaaggtggg caaaagcttc ataaacactt gagacaattt tcgagaagcg ggaagcgcaa
      901 ggtcattcac catgacggga tatttatctt ccacacgtgt tactttgcgt tcacgttctc
      961 cggtaaaaag gttttgctcg tattcgacga tttcccctga ataatcaatc tcgccaccaa
     1021 ggtcgaaggt ccacatggga agatacacac cacgaggaag gtctactttc ttttctggtt
     1081 taatatgatt tttttcgacc cattcaacca gcaaatgtgt agctcgtttt tggtcgatgg
     1141 caaaaggcaa aatggagttg ggcgcaataa tgtcgttcgt cttttcgtag ctgacgacat
     1201 gtggcgagtc gcagtaggca cagttcgaag aaatttggtt gggtggcaaa ataaattctg
     1261 caccacaacc attgcaatga aagacttgtt cttgcaatgg tttgccatgt cccttcatgg
     1321 ttgccattgc tacaatgaaa tctttttcgt tctcggttcc gctgcctgcc cccaacggat
     1381 tctgacgcgt acaataatca cacaccaacc cagaaccatc gggcgagaac gccatgcgtc
     1441 caccgcactt ggggcacatg aaacggtctg cttgcgcttc gcgcaaccct tctggcgcgg
     1501 caggcagttt atcggggttg atcacatcac ccgctttgag tttgccatct aaaatcgcga
     1561 gcgaacgacg cgcacgcgta tgatgcaagt catgcgaaag acaattctcc aatgctttgc
     1621 gtttctcagc agggtcatcc accacttcgc tcatccaata ccaagcttct gccaacacgt
     1681 catgcgaacc tgccatgtaa atggcgcggt ctaaataccg gcgggctgat tctttactgc
     1741 cggttttggc atcggtaata cctgaacgaa gaagttcttt tgcgtaatca gcgctcattg
     1801 accctcacca ttgcatccgt catttttgcc acacgcacca tctcttttac atcatgcaca
     1861 cggataatat ccgcaccacg cgtaataccc accgccacgg ttgccgccgt cccttccacg
     1921 cgctggtcgg gcggcaagtc taatgtaaaa ccaataaatg attttctgga tgtacccaat
     1981 agaattggat accctaacga gcggatctct gcgagtcggt taatcagctc gaggttgtgc
     2041 tcgcgcttct ttccaaagcc gatgcctggg tcaagcataa tgagtttttc ctccaccccg
     2101 gctttaacag caatctctac actggcaagt aattcacgtt tcacatcttc tagcaaatca
     2161 tcgtactctg cgccgaggta tgttccgccc aatttctcgc ggacttccac actggcagga
     2221 ttactgcgat tgtgcatgag aatgaccggc actttatgct tcgccgccac cgatgcgagt
     2281 tctgaatctg cacggagccc ccacacatcg ttgatgatgt tcgcgcccgc ttgaatggct
     2341 gcctctgcaa ctttggcttt gtaagtatca acagaaatca aaacctgcgg gaaatttttt
     2401 cgcaattcct cgatgaccgg aatgacacgt tccatttctt cttcggcatt gaccggctcc
     2461 gaacccgggc gcgtggattc gccgcccaca tcgagaatgt ccgcaccgta gaggatgaac
     2521 tcacgcgcct gttccactgc cgctttgacc acatcgcctt tggcaatgat cccatcacca
     2581 gaaaacgaat cgggagtcac attcaagatt cccatcacat aggtgcgaga accccaatcg
     2641 aatgtaaaac tcccgacttg aagggttgta gatttcatgt gtataaaagg ctcttgatga
     2701 ggttaaacct catcaagagc gaatttgtaa attacgcgat ctggtccaac gggcgtgcaa
     2761 gccaagcttc cgcttcgttg atgtcggtga atattttcat cgccgccgca gactcggtct
     2821 ctgccaatgc tgctactttg cgaatgccat ctgccacggc ttcgctcgct accactaccg
     2881 ccacacgaat gttccgcgct gaggggtcac tgtttgcgtc taccgccatg cgaatggctt
     2941 tctctggaat ctcaggaaca gaacgcagat cgtagagatt gcgcgtacgg cggtctgcgc
     3001 caagaagatg gtcaagggcg aattgatgcc agtgttgcat cgttgcatca gaaaggtcgg
     3061 tgaacgtaag ggtcatgcca ccatcgccgc gtcggacgac tgaatagccg attccggggg
     3121 tatctgtcca gttaagaggt ttggggtcgc tcatgagtgg tctcctattt tattaatcca
     3181 ttatacccag cgttggcaaa aaatcgtttg cgtcaatttt caaatcacgt gctataatgc
     3241 ccgtcgccgg gtgcttagct cagttggtta gagcacttcg tttacaccga aggtgtcggg
     3301 ggttcgagtc cctcagcgcc cacaaaagcc tcatgtaacc atggggcttt tcattttcct
     3361 gtgttggggg ttccccttac agggacaggc gagtccctca g
//
LOCUS       DAOQFT010000257         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16148_length_3395_cov_6.149102, whole genome shotgun sequence.
ACCESSION   DAOQFT010000257 DAOQFT010000000
VERSION     DAOQFT010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16148_length_3395_cov_6.149102"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLR93_07470"
     CDS             <1..564
                     /locus_tag="PLR93_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNH26841.1"
                     /translation="GEFAAQIMLDIPSKIIKDASRPEPVDDILAILRATKPKVVYTHN
                     LADKHDTHVGVTLRVIEALRKLSPAERPERVVGCEVWRALDWMVDSDKVTMDLSRHEN
                     LQFALLGVFDSQIVGGKRYDLASMGRRRANATYFESHGVDNAEGLSYGVDMTALMNDV
                     SIDPAKFIGDFMQRFKDDVNQRIIRMS"
     gene            695..2032
                     /locus_tag="PLR93_07475"
                     /pseudo
     CDS             695..2032
                     /locus_tag="PLR93_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            2096..3250
                     /locus_tag="PLR93_07480"
     CDS             2096..3250
                     /locus_tag="PLR93_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNH26842.1"
                     /translation="MPENPYLNVLLMAALTVVFIIACSYGLGHLAKSIARLRGASPKK
                     QESTFAGYFFAAPWIVGFLIFVIVPLGFSLYWSFTDYRIASGEPTLWVGAKNYQTILF
                     DDESFRSSIINTLYLTVIGLPLQIGVALFLAVLLNQKMRGERIFRMAFYLPVILGFNT
                     AVLLCWRLMLNTGTGIINQIIRGITEAFPPFGYISRGIIYIQEVSAAFFLGLNTGKYN
                     LMMKVIEAGFPAENRVPLGVQSPLWTKMSVVLLMVWGCGAMMLIFLAGLNNIPKELHE
                     AAEVDGASPWQRFWKISFPLLTPYIFYNLVVGMISALQIFEPIFVLYRDNQPVVSSTI
                     SMVYYLWQKSFSHFEIGYGSAISWVILVIIMIVTVIQFRLQDRWVTYDIY"
BASE COUNT          790 a          828 c          844 g          933 t
ORIGIN      
        1 ggtgagttcg cggcacagat tatgttggat attccgagca agatcattaa agatgcatct
       61 cgcccggaac ctgtagacga tattttggca attttgcgtg ccaccaagcc gaaggtggtc
      121 tatacccaca acttggcaga taagcacgat acgcatgtgg gagtcactct cagagtgatt
      181 gaagctctca gaaagctgag cccggctgaa cgtcccgaaa gagtcgtggg gtgtgaagtc
      241 tggcgtgcgt tggactggat ggtcgactcc gataaggtca ccatggactt atcgcgtcat
      301 gaaaatcttc agttcgcttt attaggtgtg ttcgactcgc agatcgtggg cggtaaacgc
      361 tacgatctgg catcgatggg acgtcgccgt gcaaatgcta catattttga atcgcatggt
      421 gtggataatg cagaagggct gtcttacggt gtggatatga cggctctgat gaatgatgta
      481 agtatcgacc cggcaaaatt tattggtgat tttatgcaaa gatttaagga tgatgtgaat
      541 cagcgaatta tcaggatgag ttgatcctgt tttattcgga aagaagcgtt agcgaagaaa
      601 gacgcttgct caggaaaaag aaattaaaaa cataaggaga agaaaaatgc aaaagaaact
      661 gttcgttttg ttatcgcttt tggtgatggc tagcatggtc ttggctgcct gcggtacccc
      721 cgccaccgaa gctcctgctg ctaccgaagc ctcatccact gaagctgccg gtacagaggc
      781 tcccgcggct acggaagccc ctgcaaacgc agaggtaacc ttgaaggttt acctgcttga
      841 ttatacccct gacaccatcg catggttaaa ggaagagatc aaccctgcct tcgaagctgc
      901 tcaccctggt gtgacggttg aaattaccga aggttcctgg tctggttggg ataccacctt
      961 cagtggcttc tttgctgctg gtgaaggtcc cgatatcatc aacctcggtt ccgaaatgaa
     1021 cactctgtat ggcgaaaatc ttgccaacat ggatgaatat ctcggcgaag gcgcttggga
     1081 cgaaatttcc aatttcggtc ctgcgcttga gaatgccaaa tactaaggct cactccgcgg
     1141 tttgccgatc ttcactgctc ctcgctacgt gttctgccgc accgacttga tggaatccgc
     1201 tggctggacc actggcaccc ccaagaactt tgaagaatgg aagacctttg cttctcaggc
     1261 ttctgtgatc gaccccgcta ccaactccct gtctcagcaa gctctcgtcc ctgtggacgc
     1321 tggctccatg gcggattggc aatggtggtt actcgtgttc tactccctcg gtggcgaact
     1381 ctacaaagcc gatggcaccc ccaacttcga ctctcctgaa gcgttggctg cgactcagtt
     1441 cttgctcgac atgcgccaag cgacctatgg cgaagctgtg aacgctgtcg gttctctccc
     1501 cgccggtcag ggctctgtga tcgacgttga tgatgccact ggtaaggata acggcgccgt
     1561 gtgtctcgca cactccggtt gggctgctcc tgcttttgat cgccccatct gggacaatgt
     1621 ttccatcgaa ccgttctatg gtgaccctga gaacttcccc aacagcaagc ccgttgtgct
     1681 cgcattcaat gactggctcg ccgttcctga atacagccag aacaaagaac tcgctgccga
     1741 atggctcaag atggctttca gcaaagaagg caacaacaag tggaatgaga ccatgggctt
     1801 gatccctgcc cgtaacgact cacagtatgg ttatgtgacc gaatctgccc agctccagcg
     1861 tgaagcggaa ttggcttccc agtatggtgt cggtttcgcc ggcatcaagg aagctgccaa
     1921 actctccacc atcatgcagg atgctctcgg caagttgatt actgaagaac ttactgttga
     1981 cgaagtgaac gcaaagatcc aggaagaata tactgccgct ctcggtcagt aaagaaactt
     2041 tattgctcgc atggggcttg ccccatgcga gcaatatttt tagaggagac tcgccatgcc
     2101 tgaaaatcca tatctgaatg tgctgcttat ggcagcattg acagtcgtgt tcatcattgc
     2161 atgtagttat gggttgggac atctagccaa gtctattgcc aggctacgcg gcgcatctcc
     2221 gaaaaaacaa gaaagtacct ttgcgggcta tttttttgcc gcaccatgga ttgttggctt
     2281 ccttattttt gtgattgttc cactcgggtt ttctctctat tggagtttta ccgattaccg
     2341 tatcgcctca ggcgagccta ccttgtgggt gggggcaaaa aattatcaaa ctattctttt
     2401 cgacgacgaa agttttcgct cttccattat caacacctta tatctcactg tgattggttt
     2461 gcctttgcag atcggcgtag cattgttttt ggcagtgttg ctcaaccaaa aaatgcgtgg
     2521 tgagcgtatc ttccgtatgg cgttttatct ccctgtgatt ttgggcttca acacggctgt
     2581 ccttttgtgt tggcgcttga tgttgaacac aggcacgggc attattaatc aaattattcg
     2641 cggaattacc gaagctttcc cgccatttgg ctatatcagc cgtggcatta tctatataca
     2701 agaagtttct gccgcattct tcctcggctt gaatactggc aaatacaacc tgatgatgaa
     2761 ggtgatcgaa gcaggctttc cggctgagaa ccgcgttcca cttggggtgc aaagcccgtt
     2821 gtggacaaaa atgtcggttg tcttgctcat ggtctggggc tgcggcgcca tgatgttgat
     2881 ctttctcgcg ggattgaaca atattcccaa ggaattgcat gaagccgccg aagtagacgg
     2941 agctagccct tggcagcgtt tctggaagat ttccttccca cttctcaccc cgtatatttt
     3001 ttataacctt gttgtaggca tgatcagtgc cttgcagatc tttgaaccga tcttcgtcct
     3061 ctatcgtgat aaccaaccgg tggtttcttc aaccatttcc atggtttatt acctttggca
     3121 aaagagtttt agtcatttcg aaatcgggta tggctctgcc atttcatggg tcattcttgt
     3181 cattattatg atcgttacag ttatccagtt ccgcttgcaa gaccgctggg tgacctacga
     3241 tatttattag gagagcacaa tgaaaaaata tgttcccatt ctcaaaagca ttgtgcttta
     3301 caccttgttg accggggcga cggtcttgat gagtttccca ctgttttgga tgatctcctc
     3361 ctcattcaaa acagtggagg aaaccaactc gccgg
//
LOCUS       DAOQFT010000258         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16285_length_3374_cov_6.115697, whole genome shotgun sequence.
ACCESSION   DAOQFT010000258 DAOQFT010000000
VERSION     DAOQFT010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16285_length_3374_cov_6.115697"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..468
                     /locus_tag="PLR93_07485"
     CDS             154..468
                     /locus_tag="PLR93_07485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNH26843.1"
                     /translation="MYIFQVHHYIKPEGIDSYKAVTLENVQKTRLEPGVLRFEFFQDA
                     VNPCHFSLFEVYADMAARDAHLQTEHFLKWKDVYLATQERKGDGHEFIAVYPEEGEWK
                     KD"
     gene            complement(512..1339)
                     /locus_tag="PLR93_07490"
     CDS             complement(512..1339)
                     /locus_tag="PLR93_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH26844.1"
                     /translation="MYKSIPEWMRMNPTSAFFLLTFVLAWMIWLPIGYLAPKYFLLAI
                     IPGAWAPTIVSVLLTWLTDGKSGVRQFLSRVIRWRVSFQWYLVVLFGIAVIAFAAQGL
                     NIWLFDGEPVKLNLPAGIPENGLLIALPIIFFVNIFFGGPLAEDIGWRGYALPKLREK
                     MNAFTASLLIGIVWVLWHLPFFWIPEGKIAVGGIPLLWFALLTTAWSVLFAWVYVNTE
                     SILMPVLFHAAVNTTLGTLGILGQANGSLTPVILNTVLTWLLVGVIVFFTGRDLKKK"
     gene            complement(1395..2009)
                     /locus_tag="PLR93_07495"
     CDS             complement(1395..2009)
                     /locus_tag="PLR93_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNH26845.1"
                     /translation="MRRTKEEAAVTRATLLKAALTLFSTKGYAATSIDDITQAAKLTR
                     GALYHHFTNKADLYNTLIEDVSARGADIVQGAVAEGGTFLDILKRVFVRQLAYIEESK
                     EARAVMELALVKTGAHPELQSGRDRQIESGKLMVASLAQTMQIGIASGELREDIPPME
                     MARSFIAFQNGVIQLWLASPKSFSLKGRAAVFADVLIQGLKKTA"
     gene            complement(2052..3023)
                     /gene="holA"
                     /locus_tag="PLR93_07500"
     CDS             complement(2052..3023)
                     /gene="holA"
                     /locus_tag="PLR93_07500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNH26846.1"
                     /translation="MPTIFLLYGNDEFAITRKLKDFESDFTDPTTADMNTTRLEARSM
                     TENDLNNAVNAMPFLAPKRLVFIAYPSGKFTKPDARKKFQEFLEKAPDTSKVVLYDVV
                     EKPKDAEKHWLVKWAEKNKPQVRSQAFFLPQQRDMPGWIINEAKNQGGQIEPAAAARL
                     AEMSGVDTRQAGMEISKLLAYVNWERPVRGSDVEAVCIVTSQQSVFDFVDALSQGNAK
                     TAQRLLHRLLENEDPFSLWGMVVRQFRLLLQAREILDARGNKDDVARALAIHPYVAEK
                     TTGQAGRFSMEALEGIYHRLLRIDEQVKTSQVTLDLALDTLVVELAR"
     gene            complement(3097..>3374)
                     /locus_tag="PLR93_07505"
     CDS             complement(3097..>3374)
                     /locus_tag="PLR93_07505"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HNH26847.1"
                     /translation="LAVHPGCGTNMATTALLAGTFAWFPLKGTKSTLWRLMLVPFALL
                     FALAGYQLSKPLGPWLQKYVTTEANLGSLQIIDIVPVRKGVHRVITK"
BASE COUNT          895 a          857 c          789 g          833 t
ORIGIN      
        1 tgcttaattt tctatgcttt gctccctatt gtcttttatt ggcactcatg gcatttagcg
       61 ccatccctac aggaaattaa cctcttgcaa tgatattgca tagtttgcct ctattcgttt
      121 ctattaaagt agaaacaaca attggaggtt gcgatgtata tatttcaagt tcatcattac
      181 attaaacctg aaggaattga ctcctacaaa gctgtcacgc tggaaaatgt gcaaaagact
      241 cgcctggagc ccggtgtgtt acgttttgaa tttttccaag atgccgtcaa cccctgtcac
      301 tttagtctct ttgaagtcta tgcggatatg gctgcccgag atgcacacct gcaaacagaa
      361 cacttcctga aatggaaaga tgtatacctc gccacgcagg agcgcaaagg cgatgggcat
      421 gaatttattg cggtctatcc agaagaaggg gaatggaaaa aagattaaca cttgagccag
      481 ctttttagaa agctggcttt ttttacttca tttatttctt tttcaaatct ctgccggtaa
      541 agaatacgat cacacctact aacagccatg tcaatacagt gttcaagatg acaggagtca
      601 gacttccatt agcttgaccc aatatgccca atgttccaag cgttgtattg acggcagcat
      661 ggaacaagac cggcataaga atgctttctg tgttgacata aacccaggca aacagaacag
      721 accacgccgt cgtcagaagc gcgaaccata gaagcggaat cccgcccaca gcaatctttc
      781 cttccgggat ccaaaagaaa ggcaaatgcc agagcaccca cacaatgccg atcaataggc
      841 ttgctgtgaa ggcattcatt ttctcgcgca gttttggcaa agcatagcca cgccagccga
      901 tatcttctgc caatggtccg ccgaaaaaaa tattcacaaa gaaaatgatg ggcagggcaa
      961 tcaacaatcc attctcgggg atacctgcag gaagatttaa cttaaccggt tcgccatcga
     1021 atagccagat gttaagtcct tgtgcagcaa aggcgatgac tgcaatgcca aaaagaacta
     1081 ccagatacca ttgaaaacta acgcgccagc ggatgaccct gctcaagaat tgacggacgc
     1141 cactctttcc atctgtgagc catgtcaaca atacagatac gatcgtcggc gcccatgcac
     1201 cagggatgat cgccagcaag aaatatttcg gcgcgagata gccaatgggg agccagatca
     1261 tccatgcaag aacaaaggtc aacaaaaaga aggcagaggt cgggttcatc ctcatccatt
     1321 cgggaattga tttgtacatg gtattctcct ttttactttt acatactact cagtatgtaa
     1381 attgattaaa aaaattaggc ggtcttttta agtccctgga tcaacacatc ggcaaagact
     1441 gccgccctgc ccttcaacga gaaagatttc ggcgaagcaa gccatagctg aataacacca
     1501 ttctgaaatg caataaaaga acgagccatt tccatgggag gaatatcttc gcgtaactca
     1561 ccactggcaa tgccgatctg catggtttga gcaaggcttg caaccatcaa cttgcctgat
     1621 tcaatttgcc gatctcgccc cgattgcagt tcaggatgcg cccccgtttt gaccaatgcc
     1681 agttccatca cagcgcgcgc ttctttgctc tcttcaatat aagcaagctg gcgaacgaac
     1741 acccgcttca agatatcaag gaacgttcca ccttccgcca cagcaccttg cacaatgtct
     1801 gctccgcggg cagagacatc ttcgatcaac gtgttgtaaa gatcggcttt gttggtgaag
     1861 tgatgataca acgcaccacg agtcaacttt gcagcttggg tgatgtcatc aatcgacgtc
     1921 gccgcatagc ccttggtgct aaaaagggtt agggcagctt tcaaaagggt ggcgcgggtg
     1981 actgccgcct cttcttttgt acgtctcata tatacatact aatcggtatg taattgtatg
     2041 tcaagagaga tttatcgtgc cagttcaaca accagcgtgt caagagctag gtctaatgtg
     2101 acctgacttg tttttacctg ctcatcgatc cgcagcagac gatggtaaat tccctccaag
     2161 gcttccatcg aaaaacgccc tgcctgcccg gtggtctttt cggcaacata tgggtggatc
     2221 gccagcgcgc gcgccacatc atctttatta ccgcgcgcat ctaaaatttc gcgtgcttga
     2281 agcagcaacc gaaattgacg taccaccatc ccccacaacg agaatgggtc ttcattctct
     2341 aacaaacgat gcagtaacct ttgcgccgtc ttggcattgc cttgggataa ggcatcgaca
     2401 aagtcgaaca cactttgctg ggatgtgacg atacaaaccg cctccacatc ggacccgcga
     2461 acgggtcgct cccagttgac atacgccaac agctttgaga tctccatgcc agcctgacgg
     2521 gtatccacgc cagacatttc agcgaggcgc gctgctgcag caggctcaat ctgcccaccc
     2581 tgattcttcg cttcgttgat gatccacccg ggcatgtcac gttgttgcgg caggaaaaaa
     2641 gcttgagacc tgacttgcgg cttgttcttc tcagcccatt tcaccagcca atgtttttca
     2701 gcatccttgg gtttttccac cacatcatac aaaacaactt tggaagtatc aggggctttt
     2761 tctaaaaact cctgaaattt cttgcgggca tcgggttttg taaacttacc cgaaggatac
     2821 gcaatgaaca ccaaacgctt cggcgcaagg aacggcatgg cattgaccgc attgttgagg
     2881 tcgttctctg tcatggagcg cgcttcgaga cgagtcgtgt tcatgtccgc agtggtggga
     2941 tccgtgaagt ctgactcgaa gtctttgagc ttgcgcgtga tggcaaattc gtcgttgccg
     3001 tagaggagga agatggtggg cataattttt ggtaggggcg gcgtcctgcc gccctaagga
     3061 aaaagggcga ggagacctcg cccctacata tataaattac tttgtaatta ctctgtgcac
     3121 acccttgcgg acaggcacta tgtctatgat ctgcagggag ccgagattcg cttcggtggt
     3181 gacgtatttc tgcagccacg gtccgagcgg tttgctgagt tgatagcctg ccaacgcaaa
     3241 gagcaatgcg aacggaacga gcatgagacg ccacagcgta gatttggtcc ctttaagcgg
     3301 gaaccaggca aaggtcccgg caagaagcgc agtggtcgcc atgttggttc cgcagcccgg
     3361 gtggacggcg agtc
//
LOCUS       DAOQFT010000259         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16353_length_3366_cov_6.623377, whole genome shotgun sequence.
ACCESSION   DAOQFT010000259 DAOQFT010000000
VERSION     DAOQFT010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16353_length_3366_cov_6.623377"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PLR93_07510"
     CDS             complement(<1..128)
                     /locus_tag="PLR93_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNH26848.1"
                     /translation="MSIQPARTRFAPSPTGHLHLGGARTALYAYLLAKKTGGQFILR"
     gene            complement(148..1275)
                     /locus_tag="PLR93_07515"
     CDS             complement(148..1275)
                     /locus_tag="PLR93_07515"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNH26849.1"
                     /translation="MTRGLSWRNFDFLLLGAVILASAFGVVMIRSAIAGNEVLAISSS
                     RQIYFAMLGVVLIFFLASIDYRYWLALDRPIHLVILGFLVTLTGFGQTAFGAQRWFQV
                     GVLFLQPTEFAKIVALIVLAKYFDSVRDQPRDLRWIFGAILRAMWIIGLILAQPNLSN
                     VMVLSVILAVMLWVNGIQVKHVITAVLLGALAVISVIALSVLGVRIPFLQAYQQERIV
                     NFVVPDPNDTYGNRYNVQQALIAVGAGGFSGEGYGYGTQTQLRFLKVRHTDFIFAATS
                     EEFGFVGSVSIILLLGVVVWRCIRAAQKARDVAGAMLAIGVATLIFFQGAVNIGMNLN
                     LLPVSGLPLPFVSYGGSGLTALMIAIGLVESVVMRQKDLEF"
     gene            complement(1338..1589)
                     /gene="minE"
                     /locus_tag="PLR93_07520"
     CDS             complement(1338..1589)
                     /gene="minE"
                     /locus_tag="PLR93_07520"
                     /inference="COORDINATES: protein motif:HMM:NF001422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNH26850.1"
                     /translation="MDFFTRKRSATSAKERLQLVLVHDRTDLTPGQLESLKDELLKAI
                     SKYIEIDPEAVRIELEKDGREQRLVADIPLKGATRHRAG"
     gene            complement(1591..2388)
                     /gene="minD"
                     /locus_tag="PLR93_07525"
     CDS             complement(1591..2388)
                     /gene="minD"
                     /locus_tag="PLR93_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNH26851.1"
                     /translation="MTAQVITVTSGKGGVGKTTAVANLATALAMDGKRVVCIDGDIGL
                     RNLDVVMGLENRIVYDIVDVIEGRCKLKQAMIRDKHYQDMYLIPAAQTRDKNAVSPSD
                     MIRICNDLRPDTDFIVIDSPAGIERGFRNAIAAADSVLVVTNPEVSAVRDADRVVGIL
                     EAEEKGNPALIINRLNPALVKNNDMLSAEDVLDLLGIRLIGIVPEDENVIIGSNRGTP
                     IAADGKSRAGQAFRNIAKRLQGVDVPFMDLTPQSGLWNTIQRLAGRK"
     gene            complement(2390..3088)
                     /gene="minC"
                     /locus_tag="PLR93_07530"
     CDS             complement(2390..3088)
                     /gene="minC"
                     /locus_tag="PLR93_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNH26852.1"
                     /translation="MEPTTSLVQIKGIRDGLLATFTEAAWEEQRNALMEQINERVSFF
                     QGARLAMDVGTQVLKVNDLVDLRDWLSERNITLWAIVSESPTTETTAQLLGLATRISK
                     PRPEETRHVHEAVSSDTALFVSKTVRSGTRIEYPGTIVVFGDVNPGAEIVAEGNVIVW
                     GRVRGVIHAGVKGDRSAFICALDLSANQLRIANESSAMLKPQKDPKPEIASINSEGKL
                     QAELWQTGETLIRK"
     gene            complement(3142..>3366)
                     /locus_tag="PLR93_07535"
     CDS             complement(3142..>3366)
                     /locus_tag="PLR93_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26853.1"
                     /translation="VARKADRCNFGAWPTHSWTIAYAPYEDPEIAIIAFAYNGGEGAS
                     VAGPMVARMIKAYFEIKALDIAQGVNVPAP"
BASE COUNT          955 a          873 c          731 g          807 t
ORIGIN      
        1 cgcaaaatga actgtccgcc ggtctttttg gctaacagat aggcatacaa agccgtacga
       61 gcaccaccga ggtgcaaatg cccggttggc gaaggtgcaa agcgagtgcg agctggctga
      121 atggacaaaa tttcactcct acttaattta gaattccaag tctttctgac gcatgacgac
      181 ggattctacc aaaccaatgg cgatcatcag cgccgtcaga ccactgccac cgtaacttac
      241 aaatggtaac ggcaaaccag aaacaggcaa taagtttagg ttcataccta tattcaccgc
      301 cccctgaaaa aagatcaatg ttgctacgcc aatagcaagc atggctcctg caacatcacg
      361 cgctttttgt gccgcacgaa tacagcgcca aacaaccaca cccaacaaaa ggatgatcga
      421 tacagaccct acaaagccaa attcttctga tgttgccgca aagataaagt ctgtatggcg
      481 cactttaaga aagcgtaatt gtgtttgtgt gccgtaccca tacccttcgc cagaaaaacc
      541 gccagccccc acagcgatca gagcttgttg cacgttataa cggttaccat aggtatcatt
      601 tgggtcggga acgacgaagt taacgatacg ctcttgttga tatgcttgta aaaacggaat
      661 ccgtacacca agcactgaaa gggcgatcac actaatcacc gctaaagcac ccaacagcac
      721 agctgtaata acatgcttta cttgaatccc atttacccaa agcatgactg ctaaaatgac
      781 cgaaagaacc atcacattac ttaagtttgg ctgcgctaga atcaaaccga tgatccacat
      841 ggcgcgcaga atagcaccaa aaatccaacg taaatcacgt ggttggtcac gaactgaatc
      901 aaagtacttg gcaagcacaa tcaatgcaac aatcttggca aactcggtag gctgcagaaa
      961 gagcacacct acttgaaacc agcgttgggc accgaatgcg gtttgaccaa aaccagtcaa
     1021 tgtgacgaga aagcctagaa taacaaggtg aattggacgg tcaagcgcga gccaataacg
     1081 ataatcaata gaagcgagga agaaaatcaa caccacgcca agcatggcaa agtaaatctg
     1141 gcgtgacgac gaaattgcca gcacttcatt tccggcaatc gccgagcgga tcatcacaac
     1201 gccaaaagca gacgctaaaa taactgcgcc taacaaaaga aaatcgaaat ttctccaaga
     1261 aagtccacgt gtcataataa aaaacatgga tgaacccgta tcgctacagg ttgatccatg
     1321 tcctattcaa aaacatatca acctgcgcga tgacgcgttg cgccctttaa tggaatatct
     1381 gccaccaagc gttgctcgcg tccatctttt tccaattcaa ttcgaacagc ttcaggatca
     1441 atctcaatat acttggagat tgccttcaag agttcatcct tcaaagattc taattgccca
     1501 ggggtcaggt ccgtacggtc gtgaaccaac accaactgta atcgttcttt tgcgctcgta
     1561 gcgctccgct tgcgtgtgaa gaagtccata tcacttcctt ccagccaaac gttgaatggt
     1621 attccacaag ccactctgcg gagtaaggtc cataaatggg acatcaactc cttgcaagcg
     1681 tttggcaata ttacggaaag cctgccccgc acggctcttt ccatcggctg caatcggagt
     1741 cccgcggttc gaaccgatga ttacattttc atcttctggc acaataccaa tcaaacgaat
     1801 gcctaaaaga tccaacacat cttccgcaga aagcatgtcg ttgtttttca caagagcagg
     1861 attcagacga ttgatgataa gcgccggatt gcctttttct tccgcttcaa gaatgcctac
     1921 cactcggtct gcatcgcgca ccgcactgac ttcggggttg gtcactacga ggacgctgtc
     1981 tgctgcggcg atggcgtttc gaaatccgcg ctcgatacct gcaggggagt caatgacaat
     2041 gaaatcagta tccggtctga ggtcattgca gatgcggatc atgtcagatg gcgagacagc
     2101 atttttgtca cgtgtttgag ccgccggaat caggtacata tcctgataat gcttatcgcg
     2161 aatcattgcc tgtttgagtt tgcaacgtcc ttcgatgaca tccacaatat cgtagacgat
     2221 acgattctca agccccatca caacatcaag gttacgcaac ccaatatcac cgtcaataca
     2281 aacgacccgt ttgccatcca tggcaagagc cgtagcaaga ttggcaactg cagtggtctt
     2341 tcccacgccg ccttttccag aagttacagt gatcacttga gctgtcatat tatttcctaa
     2401 ttaaagtttc gccggtctgc cataattctg cttgcaattt accttcgctg ttaatgctgg
     2461 caatctcggg ctttgggtct ttctgtggtt tcaacattgc ggatgactca tttgcaatcc
     2521 gtaattggtt tgcagatagg tctagggcac aaataaaagc ggagcgatct ccttttacgc
     2581 cagcatggat cactccacgc acacgccccc aaacgatcac atttccttct gccacgatct
     2641 ctgcgccggg gttcacatct ccaaagacga caatcgtacc cggatattct atccgggtgc
     2701 cagaacgaac tgtttttgaa acgaaaagtg cagtgtcgct tgatactgcc tcatgcacat
     2761 gacgggtttc ttcagggcgt ggcttggaga tacgtgttgc caacccaagt agctgggctg
     2821 ttgtttctgt ggtcggcgat tcactgacga ttgcccacaa ggtgatattt cgttctgaaa
     2881 gccaatcgcg aaggtcaaca aggtcattga ccttcaatac ctgagtccca acatccatgg
     2941 caaggcgcgc tccttgaaaa aaggaaacac gttcattaat ttgctccatc agagcattgc
     3001 gttgttcttc ccaggcagcc tcagtaaagg tcgcgagtag accatctcga atgcctttga
     3061 tttggaccag ggatgttgtg ggttccatag acatgttcat ttaggtaaca ggcattaatt
     3121 atacctgatg aacttaccac gctatggcgc tggtacgttt acaccctgag caatgtctaa
     3181 tgccttgatt tcaaaatacg ccttaatcat acgagcgacc ataggacccg ccacactggc
     3241 accttcaccg ccattatagg caaaagcaat aatagcgatc tccgggtctt catatggagc
     3301 atatgcaatc gtccatgagt gagtgggcca ggcgccaaaa ttacaacggt ctgctttgcg
     3361 ggcaac
//
LOCUS       DAOQFT010000260         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16402_length_3359_cov_5.948850, whole genome shotgun sequence.
ACCESSION   DAOQFT010000260 DAOQFT010000000
VERSION     DAOQFT010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16402_length_3359_cov_5.948850"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLR93_07540"
     CDS             complement(<1..798)
                     /locus_tag="PLR93_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH26854.1"
                     /translation="MFGSKTQALQQRAQKVAPLGVNSNFRFWGDGITPYVEKAKGGYL
                     WDVDGKKYIDYRMAFGPIILGHAYAEVDQKVIEEIQKGILFAMTGELEIAVAEMIVEM
                     SPAIEMVRLACSGTEATMHAIRVARAYTGRDIILKFEGNYHGFHDHTLWSTYAPVEAY
                     GNRRSPIPVPASSGIPKMMRENIITLPFNDFEGFERVMRSYGEQIAAVISEPCQGNCA
                     AINPQDGFLQLIRKKTEEYGCVFILDEVKTGFRIANGGAQEYYRLKPD"
     gene            complement(844..1350)
                     /locus_tag="PLR93_07545"
     CDS             complement(844..1350)
                     /locus_tag="PLR93_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNH26855.1"
                     /translation="MYEFDKTDIKIVNLLLEDGRMSASEMSRRMGDISERAVRYRVDR
                     MIEEGVIQISAVVNPEALGFNIKADVWLEVEADAVLEVAKKMASFENVTYVACGIGQN
                     DISIQLVARDTSEIYYFVTEVVRKVPGVRKTTTSIVPIILKDVYQWRVPERIAKELSE
                     NETVTASK"
     gene            1483..3039
                     /locus_tag="PLR93_07550"
     CDS             1483..3039
                     /locus_tag="PLR93_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNH26856.1"
                     /translation="MKISLLEIKEPKILKASEIKIELTTTPVKYYRHGWQSWSLTAWT
                     DLSPLPMQKPAIFHPLQTDAVYANEKNPHGSWLGAVELEDGKILLLGALATDTHVFLV
                     GNQLEGRSEAGEVEWLIAIGEEKTVFDEYVHQLGLRFGRVDKNHIPRVWCSWYSLYIA
                     IDEKILHESFDGLGNLPFDVLQVDDGWQNDIGDWEANEKFPSGMQALAEKIKSTGRRA
                     GLWLAPLLVAKSSRLYREHKDWLLRDEKGRFVSAGFNWGQQLYALDTTHPDVISWLVA
                     LMKLVRRWGFDYYKLDFLYAGALKGKRYKDMPREAAYRECLRYMREAMGEGSFFLTCG
                     TPILPALGMCDAIRIGPDVSHEWENYRNETLLYNFATPGTKNAIRTVVHRLWLKPLVH
                     IDPDVEYFESKENSLADEHKQQLQDLALICDFKATSDLPQWMTAQEREKVRAFLDAET
                     DSVQISRYVYKLDNRIVDFSSAVELPAPPTGFASAWGAFLGWLGNWKFVLRIIKTMDD
                     KKLKERVSRI"
     gene            complement(3070..>3359)
                     /locus_tag="PLR93_07555"
     CDS             complement(3070..>3359)
                     /locus_tag="PLR93_07555"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNH26857.1"
                     /translation="ALTNAEVTELLSQIPAWELVEVDGIRRLQRVFKLKNYAEAVAFT
                     NKLAMTAEKEDHHPLIVLEWGRVTVQWWTHVVQGLHQNDFVMAAKTNEIFG"
BASE COUNT          878 a          852 c          770 g          859 t
ORIGIN      
        1 gtccggtttg aggcggtagt attcctgcgc tccaccattg gcaatacgaa agcctgtctt
       61 cacttcatct aggataaaga cacagccata ttcttctgtc tttttgcgga taagttgcaa
      121 gaagccatct tgcggattga ttgccgcgca attgccctga cacggctcag aaataactgc
      181 agcaatttgc tcaccatatg accgcatcac acgctcgaag ccttcaaaat cattgaatgg
      241 cagcgtgatg atattctcgc gcatcatttt gggaataccc gaagaggctg gcacaggaat
      301 gggactgcgg cgattgccgt atgcttctac tggcgcataa gtagaccaca acgtgtggtc
      361 gtggaatccg tgataattac cctcgaactt gagaatgata tcgcgcccgg tgtaggcacg
      421 cgccacacgg atggcatgca tggtagcttc tgtgcccgaa caggcaagac gcaccatctc
      481 gatggcaggc gacatttcaa caatcatctc tgccacggca atttcaagtt cgccggtcat
      541 ggcaaacaga atgccttttt gaatttcttc gataactttt tggtctactt cagcataggc
      601 atgacctaaa ataatcggac caaatgccat gcgatagtca atgtatttct ttccatctac
      661 atcccataaa tagccgccct tggctttttc aacataggga gtaatgccat ctccccaaaa
      721 acgaaaatta gagttcaccc ccagcggtgc cactttttgc gcacgctgtt ggagagcctg
      781 agtctttgaa ccgaacatga ttgctccttt tatcgggttg cggcgaatcg ccgcgtagca
      841 gaattacttg gatgcggtta ctgtttcgtt ctctgacaat tctttggcta tgcgctctgg
      901 cactctccac tggtaaacat ctttgagaat gatcggtacg atcgaagttg tggtcttacg
      961 cacccctgga accttgcgca ccacttcggt gacaaaataa tagatctccg aagtatcgcg
     1021 cgcaaccaac tgaatactga tgtcattctg cccaatgccg caggctacgt acgtcacatt
     1081 ttcgaaagaa gccattttct tggcaacttc caaaaccgcg tcggcttcta cttccagcca
     1141 tacatcggct ttaatgttga atccgagcgc ctctgggttg accaccgcac tgatctgaat
     1201 gacaccttct tcgatcattc gatccacacg gtaacgtacc gcccgttccg aaatatcccc
     1261 catccggcgc gacatctcag aagcggacat acgcccatct tctagaagca aattgacgat
     1321 ttttatatca gttttgtcga attcatacat aggatttagg cttcttttcc gaaagtgaca
     1381 cgcagaatat accttacggt attttagacg tcaagggagc aaaattggcg tagttacgca
     1441 ttttccttta caatcagcgc gaaatttgat ggaaactcaa acatgaaaat ctctctttta
     1501 gaaatcaaag aacctaaaat tctcaaagcc agcgaaatca agatcgagct cacaaccacc
     1561 ccggtaaagt attaccggca tggctggcag tcttggtctc tcacagcatg gacggatctc
     1621 tctccactcc ccatgcaaaa acccgccata tttcacccat tacaaaccga cgcggtttat
     1681 gcaaatgaaa agaatccgca tggttcatgg ctaggcgcag ttgaacttga agatggcaaa
     1741 atccttttgc ttggcgcact tgctacagat acgcatgtgt ttctggtggg caatcaactc
     1801 gaagggcgaa gtgaagcggg cgaagtcgaa tggcttattg ctataggcga agagaaaacc
     1861 gtcttcgatg aatacgtcca tcaactaggg ttgcgatttg gcagagtaga taagaatcac
     1921 attccacgtg tatggtgctc atggtatagc ctttacatag ccatcgacga gaaaatcttg
     1981 cacgagtctt ttgatgggct cggaaacctg ccttttgatg tgctgcaagt ggatgatggt
     2041 tggcagaacg acattggcga ttgggaagcg aatgaaaaat ttccatcggg tatgcaggca
     2101 ttggcagaga aaatcaaatc caccgggcga cgcgccgggt tatggctcgc tccattatta
     2161 gtggcaaaat catcacgcct ctatcgtgaa cataaggatt ggttattgcg cgatgaaaag
     2221 ggacggttcg tttcagctgg attcaactgg ggtcagcaac tttacgcgct cgacaccacg
     2281 catcccgacg taatctcgtg gctggttgcg cttatgaagc tggtgcgccg ctggggcttt
     2341 gactactaca aactcgactt tctctatgct ggtgcgttaa aaggcaagcg ctacaaagac
     2401 atgccgcgtg aagccgctta tagagaatgt ctgcgttaca tgcgcgaagc aatgggagaa
     2461 ggttccttct tcctaacctg cggcacgccc atcctccccg ccctcggtat gtgcgatgcc
     2521 atccgcatcg gacccgacgt ttcgcatgaa tgggaaaact atcgtaacga aactctcttg
     2581 tacaacttcg ccacacccgg cacaaagaac gccattcgca ccgtagttca ccgcttgtgg
     2641 ctcaagcccc tcgttcacat tgaccccgat gtggaatatt ttgaatcaaa agaaaattct
     2701 ttggcagatg agcacaaaca gcaattgcaa gacctcgccc tgatctgcga cttcaaagcg
     2761 acgtccgact tacctcaatg gatgacggct caggagcgag aaaaagtccg cgcatttctg
     2821 gatgctgaaa ccgactcggt gcaaatcagc cgttacgttt acaaactcga taaccgcatc
     2881 gtggactttt catccgccgt agaactgcct gcgccgccta caggctttgc aagtgcatgg
     2941 ggcgccttcc tcggttggct tggtaactgg aagttcgtgc tgcgaatcat aaaaacgatg
     3001 gatgataaaa aattaaaaga acgagtttca aggatataaa ataaaagcga ccctgatggg
     3061 tcgcttttat tatccgaaaa tttcatttgt cttggctgcc atcacaaaat cgttctgatg
     3121 caagccttga accacatgcg tccaccattg gacggtaact ctgccccatt ccagcacaat
     3181 cagtggatgg tggtcttctt tttcagcggt cattgcgagc ttatttgtaa aagcaacagc
     3241 ctcagcatag tttttcagtt taaagactcg ctgtaaacgt cggatgccat ctacttccac
     3301 caattcccat gcagggattt gtgaaagcag ttcagtgacc tcagcgttgg ttaacgctg
//
LOCUS       DAOQFT010000261         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16442_length_3354_cov_9.065778, whole genome shotgun sequence.
ACCESSION   DAOQFT010000261 DAOQFT010000000
VERSION     DAOQFT010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16442_length_3354_cov_9.065778"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLR93_07560"
     CDS             <1..467
                     /locus_tag="PLR93_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH26858.1"
                     /translation="AFAKSFPQRVTGLALVSSQAASDTPERKEGRYKTAADVAQKGVQ
                     IVADAMTDKLSPNQSVRDFVRPLIAKQSVAGVTGALKAMAERDDMTSFIASYAQPLVL
                     IHGDADELIPLERAREIKSLVSSAQLVELAGVGHMPMLEMPKETASALKKLK"
     gene            complement(531..974)
                     /locus_tag="PLR93_07565"
     CDS             complement(531..974)
                     /locus_tag="PLR93_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26859.1"
                     /translation="MTPTAKILVIDDDAAITELMSMLLKTFGFEVLTTNIGEEGIRLA
                     REASPNVILLDLMMPDLDGWQVCKEIRKTSTVPILILSAINDPRMVASVLDVGADDFL
                     VKPVPSGVLVANIKKLIRRNTGALDMSKLKKTTASLQQANHALHP"
     gene            1178..>1530
                     /locus_tag="PLR93_07570"
     CDS             1178..>1530
                     /locus_tag="PLR93_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNH26860.1"
                     /translation="MSKWVYLYNEVKEVEKLAKGSWDGVKAIVGGKGAGLMDMTRANV
                     PVPPFFTVTTEACNAYQKAGKFPQGMWEQELKALKEIERKTGKKFGDPKNPLLVSCRS
                     GAKFSMPGMMNTILN"
     assembly_gap    1531..1630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1631..>3354
                     /locus_tag="PLR93_07575"
     CDS             <1631..>3354
                     /locus_tag="PLR93_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNH26861.1"
                     /translation="LGTAVNICTMVFGNMGDDSATGVAFTRNPSTGEKKMMGEYLLNA
                     QGEDVVAGIRNTDAIENLKKYMPKAYAQFMEITARLEKHYTDMQDVEFTIERGKLWML
                     QTRVGKRTAKAAVKIAVDMANEGLINKDQAVQRVTPDQVDALLHPQFNDEAMNQAEKE
                     GKFLAKGVNASPGAAVGQIYFDADKAEEYAKEHKQDTIMVRPFTKPDDVHGMIAAKGV
                     LTSEGGATSHAAVVARQFGIPCVVGATAIKIDLEKRIMHVGEIVLNEGDWVSVDGTTG
                     KVFAGKIPTSEPSLEEQKELMTLLKWADDISARKNIREEVEPGYPTRGLQVWANADYP
                     KDARRARSYGAVGIGLCRTEHMFFEPERLPIVQRMILAETSEGRTEALNLLLPHQRKD
                     FDGLFEAMDGYPVIIRLIDPPLHEFMPDEEKLLEEVITMRVKGETKDLAEKEKLLESI
                     KGMHESNPMMGLRGVRLSISMPEIVEMQVRAIFEAAADCTLRGIKVKPEIMIPLTGTV
                     KELDWIQPRLERIGKAVMQEKKIKFAYKFGSMIEIPRAAVTADHIATQAQFFSFGTND
                     LTQMTFGYS"
BASE COUNT          852 a          730 c          848 g          824 t
ORIGIN      
        1 tcgcatttgc gaagtctttt ccacagcgtg tgaccggttt ggctttggtt tcctctcaag
       61 cagcctcaga tacccctgaa cgtaaagaag gtagatataa aacggcggca gatgttgctc
      121 aaaaaggggt ccagatcgtt gccgatgcga tgacggataa actttcgccc aatcaatctg
      181 tgcgagattt tgtccgcccg ttgattgcga agcaaagtgt tgctggtgtg acgggggcac
      241 tcaaagccat ggcggagcgt gacgatatga cttcgttcat agcatcttat gctcagcctc
      301 ttgtgttgat tcacggagat gcagatgagt tgataccgct cgaacgtgcg cgggagatta
      361 agtcacttgt ctcgtctgcg cagttggtgg agttggctgg ggtggggcat atgcccatgc
      421 tggaaatgcc aaaggaaacg gcttcggcgt tgaaaaagct caaatagcaa agaaaatgcc
      481 catccagcta acggggatgg gcatttttat tttgtagaac cagtttcggg ctaagggtga
      541 agcgcgtgat tagcttgttg gagggaagcc gtcgtttttt ttaacttgga catatccagt
      601 gcgcctgtgt tgcgtcgaat caatttcttg atattcgcaa ccaacacgcc gctgggcacc
      661 ggtttgacga ggaaatcgtc tgcgccaaca tccaaaacgc tcgcgaccat gcgtggatcg
      721 ttgattgcag aaaggatcag gattggcaca gtacttgttt tgcgaatttc tttgcaaacc
      781 tgccatccgt ctaaatcagg catcattaga tcgagtagga tgacgtttgg actagcctca
      841 cgtgcaaggc gaatgccttc ttcgccaatg ttggttgtga gcacttcaaa cccgaaggtt
      901 tttaataaca tgctcatcaa ctctgtgatg gcagcatcat catcgatcac aaggattttt
      961 gctgtaggtg tcataatatc taggcacatt atccctgatt tgactgacaa tgtactttgc
     1021 aatgtattaa caggttcttt tgaataagaa ttagtaattt ccctgattga catccactct
     1081 tagcatgatt tttgtcaggt gctaattgga ctgtttttgg tacaatattt cacaatcaag
     1141 atatagagtg acaatattac tcgatcagga ggtcgaaatg tctaaatggg tttatctcta
     1201 taatgaggtg aaggaagttg aaaaattagc caaaggctca tgggatggtg tcaaagccat
     1261 cgtcggcggc aaaggtgcgg gcttaatgga tatgactcgc gcgaatgtgc cggttccacc
     1321 tttctttacc gttactacag aagcgtgcaa tgcatatcaa aaagctggga aattcccgca
     1381 aggaatgtgg gagcaggaat tgaaagcgct gaaagagatt gagcgcaaaa ccggcaaaaa
     1441 attcggtgat ccgaaaaatc cgcttttggt ctcgtgtcgc tcaggtgcta agttctcaat
     1501 gccgggcatg atgaatacca ttctcaatat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn ctcggaaccg ccgtcaatat ttgtacgatg gtcttcggga acatgggcga
     1681 tgactctgcc accggtgtgg cattcacgcg taacccgtcc actggcgaaa agaagatgat
     1741 gggtgagtac ctgctcaatg cgcagggtga agatgtggtg gctggtatcc gcaatacgga
     1801 tgccattgaa aacctcaaga agtatatgcc caaggcatat gctcaattca tggaaattac
     1861 cgctcgcctt gaaaagcact acaccgatat gcaggatgtg gaattcacca ttgaacgcgg
     1921 caagttatgg atgctccaga ctcgtgtggg taaacgcact gccaaagccg ctgtgaagat
     1981 cgctgtggat atggcaaatg aaggcttgat caacaaagat caggcggtcc aacgcgttac
     2041 gccagatcag gtggatgcct tacttcaccc gcaatttaat gacgaagcca tgaatcaggc
     2101 tgaaaaagaa ggcaagttcc ttgctaaggg tgtgaatgct tctcccggtg cggctgttgg
     2161 tcaaatttat tttgatgccg ataaagctga agagtatgcc aaagaacaca agcaagatac
     2221 catcatggta cgtccgttca ccaagccgga tgatgtccac ggtatgatcg ctgccaaggg
     2281 cgtgttgacc agtgaaggtg gtgctacttc tcacgcagcc gttgtagctc gccaatttgg
     2341 tatcccatgc gtggtgggtg ctacagccat caagatcgac cttgaaaagc gcattatgca
     2401 tgtgggtgaa attgtgctca acgaaggcga ttgggtctct gtagacggca caactggtaa
     2461 agtctttgct ggaaagatcc caacctctga accttctctt gaagaacaga aagaattaat
     2521 gaccctgctc aagtgggcag atgacatttc cgctcgcaaa aacattcgcg aagaagtgga
     2581 acccggttat ccaacccgtg gtttgcaagt ttgggcgaat gctgattatc caaaagatgc
     2641 tcgccgtgct cgctcttatg gagctgtggg tattggtttg tgccgcacgg agcatatgtt
     2701 ctttgaacct gaacgcttgc cgattgtgca acgcatgatt ctcgctgaaa ccagcgaagg
     2761 acgtactgaa gcgctcaact tgctcttacc gcatcagcgt aaggactttg acggtctctt
     2821 cgaagcgatg gacggttacc ccgtgattat tcgtttgatc gacccacctt tgcatgaatt
     2881 catgcccgac gaagaaaaac tccttgaaga agttattacc atgcgagtga agggtgagac
     2941 gaaagacttg gcagaaaaag aaaaactgct tgaatcgatc aagggcatgc acgaatccaa
     3001 cccgatgatg ggcttgcgcg gcgtgcgttt gagcatctcg atgcccgaga tcgtggaaat
     3061 gcaagtgcgt gccatctttg aagccgctgc cgactgcacc ctgcgcggta tcaaggtcaa
     3121 gcctgagatc atgatcccgt tgaccggaac cgtgaaagag ttagactgga tccagccgcg
     3181 cctcgaacgc atcggtaaag ccgtgatgca ggaaaagaag atcaagttcg cctataaatt
     3241 tggctccatg atcgagattc cgcgtgctgc tgttacagca gaccacattg ccacccaggc
     3301 gcagttcttc tcctttggta cgaatgacct tacacaaatg accttcggtt attc
//
LOCUS       DAOQFT010000262         3351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16470_length_3351_cov_5.277609, whole genome shotgun sequence.
ACCESSION   DAOQFT010000262 DAOQFT010000000
VERSION     DAOQFT010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16470_length_3351_cov_5.277609"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLR93_07580"
     CDS             complement(<1..340)
                     /locus_tag="PLR93_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26862.1"
                     /translation="MTVYSYQINGMSVQTGDIICTMNGKPDILPGEFWRLVGRLVPGD
                     VDHVAIYLGPDGRCAEAGAKGVITFDVPNGVWNTERMARQRGLLFDTFYGVVSPLDVL
                     GYMEEDEFHLR"
     gene            complement(374..1420)
                     /locus_tag="PLR93_07585"
     CDS             complement(374..1420)
                     /locus_tag="PLR93_07585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH26863.1"
                     /translation="MKIGLVIYGSLDTLSGGYMYDRMLVEHLRAQGDTVEIISLPWRN
                     YAAHLTDNFTFKLPSGLDVLIQDELNHPSLILANRRPHPYPIISLVHHLRCSELRPQW
                     QNDFYRVVEKKFLQSVDGFIFNSETTKGVVNRLIGNSSPEIVAFPPTDRFGEAIAEDG
                     IKERSQEKELNILFLGNIIERKGLHTLLQAAKLLTCDFRLDIVGGLTAEPEYAKSVQA
                     YIADNNLSSVISLHGALNNQPLVDKLKQAHVLVVPSSYEGFGIVYLEGMGFGLPAIGT
                     TLGAAPEVIDDAVTGFLIQPGDAHRLAEKLQLLSENRDLLLKMSLAARSRYQRQPKWN
                     QTANQIRDFLLSFI"
     gene            complement(1421..1798)
                     /locus_tag="PLR93_07590"
     CDS             complement(1421..1798)
                     /locus_tag="PLR93_07590"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNH26864.1"
                     /translation="MYSLGVRREFIARHFLIGGDWGPENFPNSHHYTLELVLEGKELD
                     QHGYLVDIVDVEKHLDEIVNYYKEQMLNEKPEFAGLNPSIEHFSRILATTLSERIKAK
                     NISGLKVVLWEHANAWAAFQVTR"
     gene            complement(1825..2499)
                     /locus_tag="PLR93_07595"
     CDS             complement(1825..2499)
                     /locus_tag="PLR93_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNH26865.1"
                     /translation="MKFRRYTLDDCHQATGIVLVIDVLRAFSTAAYAFSRNAKEIRLV
                     STVEEALSLKAQISNSKAIGEVGGIPPEGFDFGNSPTHILEHDLTGITLIQRTGSGTQ
                     GAVRAVNANVLLATSFVVSGATIEYVSKLQPHEVSFVITGGMDNDEDVACADYLEMKF
                     TGQQSNYEIFIRRVYESKDALQHRQEHPQFPYSDLDYCSRINYFDFAMPIKRKDGFLI
                     MQAVKP"
     gene            complement(2496..2828)
                     /locus_tag="PLR93_07600"
     CDS             complement(2496..2828)
                     /locus_tag="PLR93_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26866.1"
                     /translation="MENKPASENGFSNSFIGKLFLGGLPVLGGLLTILVSLNILPYTD
                     LRPNRIAIFNDPHTWEVFAVGATMLFFGIANILPPQMKALGRINTFLFFLSLMAVVIG
                     VLLKRFSG"
     gene            complement(2818..>3351)
                     /locus_tag="PLR93_07605"
     CDS             complement(2818..>3351)
                     /locus_tag="PLR93_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNH26867.1"
                     /translation="QGIVGLLTASLLHEFPLETLTVVDSIELRRKAMNVISRKANFKS
                     LSPNDLEPSSFDLTFELTGSPSALNTAIEHTAFSGRIVIGSWYGQKRAEIDLGGSFHR
                     SRIQLISSQVSSISPALSGRWDKSRRFEVAWQALERIQPQKWITHRFSLSEAENAYRL
                     LDENSHETIQVVFDYGK"
BASE COUNT          917 a          766 c          792 g          876 t
ORIGIN      
        1 tgcgtaaatg aaattcgtct tcttccatat acccaagcac atccaatgga gagacgacgc
       61 cgtaaaaggt gtcgaacaac aaaccgcgtt ggcgtgccat gcgttcggtg ttccaaactc
      121 cgttgggtac atcaaaagtg atcacacctt ttgccccagc ctcggcacag cgcccgtcgg
      181 gtccgaggta gatggcgaca tggtccacat cgcccggcac gagccgccca accaagcgcc
      241 aaaactcacc cggtaagata tcgggcttgc cgttcatggt gcagataata tcgcctgttt
      301 gcacagacat accgttgatc tgataactgt aaaccgtcat gttactttat acgaaggtgt
      361 acagcaaaag tttctatata aatgaaagta gaaaatctcg aatctgattc gcggtctgat
      421 tccacttggg ctggcgctga tagcgactcc gcgctgcaag gctcattttt aggaggagat
      481 cacggttctc gctcaggagt tgcagttttt ctgccaacct gtgagcgtct cccggctgga
      541 tgagaaaccc agtcaccgca tcgtcgatga cttctggtgc cgcgccaagg gtggtgccga
      601 ttgcgggcag accaaagccc atcccttcga ggtagacgat gccgaaacct tcatagcttg
      661 atggcacaac gaggacatga gcttgtttga gtttgtcgac aagaggttga ttgttgagtg
      721 caccgtgaag agagataacc gatgaaagat tattatcggc gatgtaagct tggacagatt
      781 tagcgtattc tggttcagct gtcagcccac cgacaatgtc gagccggaag tcgcaagtca
      841 aaagtttagc ggcttgcaac agagtgtgaa gtcctttgcg ttcaatgata ttgccaagaa
      901 aaagaatgtt cagttctttt tcttggcttc tttcttttat accgtcttct gcaattgctt
      961 ctccaaaccg atctgtcggt ggaaatgcta cgatttctgg cgagctattc ccaattaacc
     1021 gattcaccac gccctttgtg gtttcagagt tgaagatgaa tccatccacg ctttgcagga
     1081 attttttctc cactacacga taaaagtcgt tctgccattg cgggcggagt tctgaacagc
     1141 gcaggtgatg taccaagctg ataatgggat aggggtgtgg acgtcgattc gcaaggataa
     1201 gtgaaggatg gttcagttca tcttgaatca aaacatccaa gccagaagga agcttgaaag
     1261 tgaaattatc cgtcagatgt gcagcgtagt tccgccaagg gagcgagata atctctaccg
     1321 tatcgccctg ggcacggaga tgctccacca acatgcggtc atacatgtat ccgccgctga
     1381 gcgtatccag tgagccgtaa atgacaagac cgattttcat ttagcgtgtt acttgaaacg
     1441 ctgcccaggc attggcatgt tcccacagaa ctacttttaa accagagata tttttagctt
     1501 tgatgcgttc actcagggtt gtagcaagaa tccgtgagaa gtgttcaatg gatgggttca
     1561 gtccggcaaa ttcaggtttc tcattcaaca tttgctcttt gtaataattc acgatctcat
     1621 cgagatgttt ttctacatct acaatgtcta caagatagcc gtgttgatca agttctttcc
     1681 cttccagaac aagctccagc gtgtagtgat gcgagtttgg gaagttttct ggtccccaat
     1741 ctccgccaat caaaaagtga cgtgcaatga attcgcgtcg tacgccaagt gaatacatag
     1801 tgttctccaa agtaaaagtc aggcttaggg tttaactgcc tgcattatta aaaatccatc
     1861 tttacgttta atgggcattg caaagtcaaa atagttgatg cgtgaacagt agtctaagtc
     1921 agaatatgga aattgaggat gttcctgcct gtgttgaaga gcatcttttg actcgtaaac
     1981 acggcggatg aatatttcat agttagattg ttgacctgtg aatttcattt ccaaatagtc
     2041 ggcgcaagca acatcttcgt cattgtccat tccacctgta atgacaaagc tgacttcatg
     2101 aggttgtaat ttactcacat attcaatcgt ggctcctgac accacaaaac tggtcgccaa
     2161 taaaacattt gcattcacgg cgcggacagc cccttgtgtg ccagagcctg tgcgctggat
     2221 gagagtgata cctgttaggt catgttcgag aatgtgagta ggggagttgc caaagtcgaa
     2281 gccttccggt ggaatgccgc ctacttcacc gatagctttt gagttggaga tttgagcttt
     2341 caaggataag gcttcttcca cggtgctgac gagtcgaatc tctttggcat tacgcgagaa
     2401 agcataagca gcggtggaga aagctcgtaa aacgtcaatc acaaggacga tgccagtggc
     2461 ttggtgacag tcgtcgagag tatagcgccg gaatttcatc cgctaaacct tttgagaaga
     2521 acaccgatca caacagccat caaagaaaga aaaaatagaa aagtattaat ccttcccaac
     2581 gctttcatct gaggtggcaa gatattagca attccgaaaa agagcatggt tgccccaact
     2641 gcaaagacct cccaagtatg tgggtcgttg aaaatagcaa tacgattggg gcgaaggtca
     2701 gtatagggca gtatgtttag cgaaaccaaa attgttaata gaccacctaa aacaggcaac
     2761 ccaccaagga ataatttccc aatgaaacta ttggagaatc cgttttctga ggcaggttta
     2821 ttttccataa tcaaaaacga cctgtattgt ttcatgcgaa ttttcatcca acaatcgata
     2881 ggcattttcc gcctcactca gtgaaaatcg atgggtaatc cacttttgcg gttggatgcg
     2941 ctccaaggct tgccatgcca cctcaaaacg tcgagacttg tcccagcgtc cgcttaatgc
     3001 tggtgaaatg ctgctaacct gtgaagaaat cagttgaata cgtgagcgat gaaatgaacc
     3061 accaaggtcg atctctgccc gtttttgccc ataccatgag ccgatcacga tccgtccgct
     3121 gaatgcggtg tgctcaatgg cagtgttcaa agcagaggga gatcccgtaa gttcaaatgt
     3181 taagtcgaaa gatgaaggct caagatcatt tggtgaaaga cttttgaaat ttgccttccg
     3241 actaatgaca ttcattgctt tccttcgcaa ctcaatcgaa tcgacaaccg tcaaagtctc
     3301 aagcggaaac tcatgaagca gagaagccgt tagcagacca acgatccctt g
//
LOCUS       DAOQFT010000263         3348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16493_length_3348_cov_7.811418, whole genome shotgun sequence.
ACCESSION   DAOQFT010000263 DAOQFT010000000
VERSION     DAOQFT010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16493_length_3348_cov_7.811418"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /gene="lipB"
                     /locus_tag="PLR93_07610"
     CDS             complement(<1..614)
                     /gene="lipB"
                     /locus_tag="PLR93_07610"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNH26868.1"
                     /translation="MPLHIIGMNIEILDLGLIDYEQAWKLQDEYAGEIAKGLRPPTLL
                     LLEHPHVYTFGRKGNASNLLWNENQLKEKGIATYWVDRGGDVTYHGPGQLVGYPLIPL
                     GRLNQDNRLPEADYVGYIRKLETMLIGVLMNYGIASGQRPGKTGVWIQADVHSRCIHC
                     KPEDKQKPAKIAAIGVKVDVNGVTRHGFALNVNPDMDYWNGIIP"
     gene            complement(638..2032)
                     /locus_tag="PLR93_07615"
     CDS             complement(638..2032)
                     /locus_tag="PLR93_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH26869.1"
                     /translation="MRTTFTKLENFGHSLEAPAYVLKPRNSEEIYSAFKQAKKMGLTV
                     TARGAGRSYNDASLNGGGIVLNMTGMDQILEWDPATGLVRCEPGVTLEKLWQQVEPDG
                     WWPPVVSGTMRTTLGGCLSANIHGKNNFKMGTIGEHVVEFTATLPTGAEVTCSPKKNA
                     DLFYSMISGYGMLGIFTSVTMQMKRLHSGLLVVDALPVHNLREHLSALQDGAPHNDYI
                     VGWLETFASGKALGRGQIHAARYLHEGEDPNPQETMKLKNQILPPMIFGVFPKSILYQ
                     FMKPFANNLGWRLVNIAKYVASLPKHTFRQSHAAFHFLLDYVPNWELSYGRGGLIQYQ
                     SFLPKETAESAWREVLSLSQKRGMPSYLGVTKRHRPDKFLLTHAVDGFSLALDFKVSD
                     STRPKMAAMLQEFDKIVLAHGGRFYFAKNGETTAETAQAFYGEETVKKFKKLKKRCDP
                     DGLLESDLYRRIFG"
     gene            complement(2041..2823)
                     /locus_tag="PLR93_07620"
     CDS             complement(2041..2823)
                     /locus_tag="PLR93_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH26870.1"
                     /translation="MSDLKPATPLNPRRRAIVVGASVGMGAALSKKLAKEGYVVALVA
                     RQKEKLNELCAEINESTGEVRAFAYPHDVSNYKEAPELLKNIVAEIGGLDTFIFMAGV
                     NYPPGGNDIYNFENDRQMVEINLIGAMAWLSPVAEMFQAAKYGQIVGVGSVAGDRGRV
                     GNPGYNTSKAGLATYLEALRNRLTRHGVNVLTVKPGFVKTEMLKAAVGPTPFAIPVEK
                     AVDDIYKAMQKRKQSIYTPFFWQYIMLIIRHIPSFIFRRLSF"
     gene            2972..>3348
                     /locus_tag="PLR93_07625"
     CDS             2972..>3348
                     /locus_tag="PLR93_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26871.1"
                     /translation="MNSNHGKHGSVFTRAWDIISRDDTIPPEEKKAILRLIQVIVEAR
                     EQASDKRVGDDEEKSITHEVISKHSLMMTVKHQADELDALRRISLNLTSNLDLQKVLD
                     ILVTESMGLIKNANNVTIFLYERG"
BASE COUNT          864 a          778 c          814 g          892 t
ORIGIN      
        1 caagggatga tgccattcca ataatccatg tcagggttta cgttcaaggc gaacccgtgc
       61 cgggtgactc cgttcacatc cactttcaca ccaatggcgg cgatcttggc gggtttctgc
      121 ttatcttctg gcttgcaatg gatgcaacgc gaatggacat cggcttgaat ccatacgcca
      181 gttttgccgg ggcgttgacc agaggcaatg ccatagttca tcaaaacacc aatcaacatg
      241 gtttcgagct tgcgaatata cccaacgtaa tcggcttcgg gtaaacgatt gtcttggttc
      301 aatctcccta gtggaattaa cggatagccc accagttgac caggtccatg ataggttaca
      361 tcaccaccac ggtctaccca gtaagtggcg atgccttttt ctttgagctg attttcattc
      421 caaagtaagt tgctggcatt gcctttacgc ccaaaagtgt acacatgtgg atgctcaagc
      481 aataaaagag ttggcgggcg cagccctttc gcaatttccc ccgcgtattc atcctgaagc
      541 ttccaagctt gttcgtagtc gattaaacct aaatctaaaa tttcgatgtt cataccaata
      601 atatgtaggg gcatgattta tcatgcccct gttcaaatta tccaaatatt cttcgataca
      661 aatctgattc caataaaccg tctgggtcgc agcgtttctt taattttttg aacttcttga
      721 cagtctcttc gccgtaaaaa gcctgcgctg tttcagcggt tgtttcacca ttcttggcaa
      781 agtaaaaacg cccaccatga gctaggacga tcttatcaaa ttcctgtaac atggctgcca
      841 tcttcggacg agtggaatcg gacactttga aatccaacgc gagcgagaaa ccatctacag
      901 cgtgggtgag caaaaattta tcggggcggt ggcgcttggt cacaccgagg tacgagggca
      961 taccgcgctt ttgtgaaagg ctcaacactt ctcgccatgc agactccgcg gtctctttgg
     1021 ggaggaagga ttgatattgg atcaagccgc cgcgtccata tgaaagctcc caattcggca
     1081 cataatctaa caagaaatga aatgccgcat gtgattggcg gaaggtatgt tttggaaggg
     1141 atgccacata ctttgcgata ttcaccaaac gccaaccgag attgttcgcg aacggtttca
     1201 tgaactgata taagatggat ttcggaaaga cgccaaagat catgggcgga agaatctggt
     1261 tcttcaactt catcgtctct tgcgggttcg ggtcttcgcc ttcgtgcaaa tagcgtgcgg
     1321 catgaatttg tccacgccct aatgctttac cgcttgcaaa ggtttccagc cagccaacaa
     1381 tgtaatcgtt atgcggggct ccgtcctgca gagcagaaag gtgttcgcgg aggttatgta
     1441 cggggagcgc atccacaact aaaagtccgg agtggaggcg tttcatttgc atggttacgg
     1501 aagtgaagat gccaagcatc ccatatccgc tgatcatgga gtagaacaag tccgcattct
     1561 ttttgggcga acaagtcact tccgcgccgg tgggtagtgt ggctgtaaat tccaccacat
     1621 gttcaccgat ggttcccatc ttaaaattat ttttgccgtg gatgtttgca ctgagacaac
     1681 cgcccaatgt tgtgcgcatc gtaccagaga cgacgggcgg ccaccagcca tctggttcga
     1741 cttgttgcca aagtttttca agcgtgacgc cgggctcgca tcgcaccaag ccggtagctg
     1801 gatcccattc cagaatttga tccatgccgg tcatgttgag cacaattccg ccaccgttga
     1861 gggaagcatc gttataactt cgcccggctc cacgagctgt gacggtaagc cccattttct
     1921 ttgcttgctt gaatgcagaa tagatttctt cgctgttgcg aggttttaga acatacgccg
     1981 gggcttcaag agaatgacca aaattttcga gcttcgtaaa agtagtacgc atgaggttcg
     2041 ttagaaagag agacgtctga atatgaaaga ggggatatgc cgaatgataa gcatgatgta
     2101 ttgccagaag aaaggagtat agatggattg cttgcgtttc tgcatggctt tgtaaatgtc
     2161 atctaccgct ttttcgaccg ggattgcgaa cggagtggga cctactgcgg ctttgagcat
     2221 ttcggttttc acgaagcctg gtttgactgt gagcacattc accccatgac gggtgaggcg
     2281 gtttcgtaag gcttccagat aagttgccaa cccagctttg gatgtgttgt agccggggtt
     2341 gcctactcgt ccacggtcac cagcaacgga gccaacacca acaatttgcc catatttcgc
     2401 cgcttggaac atttccgcga ctgggctgag ccatgccata gccccaatca ggttgatttc
     2461 caccatttgg cggtcgttct cgaaattgta gatgtcgttc ccaccgggcg ggtaattgac
     2521 accagccatg aaaataaaag tatctaaacc gccaatttca gcgactatat tctttaagag
     2581 ttctggagct tctttatagt ttgaaacatc gtgcgggtaa gcgaaggcac gtacttcccc
     2641 agtagattcg ttgatttcgg cgcagagttc attcaatttt tctttttgac gggcaactaa
     2701 agcaacgaca tacccctctt tggcaagttt tttagagagc gcagctccca ttccaacaga
     2761 agcgccgacc acaatggctc tgcggcgcgg atttaatggt gttgcaggtt ttaagtcact
     2821 cacaatgacc tcgtattaag tatttattca tgtcattcta acacagcaat aaatgacctt
     2881 tcgggtcgaa atagaatatg tatatgaaag tgtataatag tacaaaggat gtctcgacag
     2941 ttaatgcttt ttaggcataa ttctaaaaaa catgaatagc aatcatggca agcacgggtc
     3001 ggtctttacg cgagcgtggg atattatctc tcgtgatgat acgattccgc cagaagagaa
     3061 aaaagccatc cttcggctca ttcaggttat tgttgaggca cgtgagcagg cttctgataa
     3121 aagagtaggg gatgatgaag agaaatccat tacccatgaa gtgatctcca aacactcgct
     3181 gatgatgacc gtcaagcatc aggcagatga actggatgcg ttaaggcgca tcagcctgaa
     3241 tttgacctcg aacttggatt tgcaaaaagt gttggatatc ctcgtgacag agtcgatggg
     3301 gttgatcaag aatgcaaaca atgtgaccat cttcttatat gaacgcgg
//
LOCUS       DAOQFT010000264         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16663_length_3324_cov_7.017131, whole genome shotgun sequence.
ACCESSION   DAOQFT010000264 DAOQFT010000000
VERSION     DAOQFT010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16663_length_3324_cov_7.017131"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="PLR93_07630"
     CDS             complement(<1..1952)
                     /locus_tag="PLR93_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH26872.1"
                     /translation="MPRTGQKKSAAPKSATKKKAVRATTKKTTSSKPTSVTQLRKELE
                     QREAELAIIKSVQDGLASKLEMQAIYDLVGEKIRDLFKAQTTIIATFDRKTETQSFNY
                     YVDRQGREYIEDRPMSGLVKALIREKKTFLFNEHVEDRMREFNAELVLGPLIPKSALY
                     VPLTTAGEVRGVISLQNMDKEHAFNKSDVRLLETLAGSLCAALENARLLNETQRLLNE
                     TEQRNAELEAIRKATINLSSSLDYTTVLNSLLQNTWTLMPSIEDINLFIYENGELKFG
                     TAIAHGKITNAPAFHPRPGGMTDIVSKSGEMMLVEDMSVDPLYANSNWGGALIGLPLK
                     FSNRVVGVMNIHFAEPRIFTESELRLLKLFGDQAAVVIENSRLFNETERLLSETAGRN
                     AELGVINSLQQVLVSKLDFQSIIDLIGDKVGEIFDAQVTLISLYYPATGEMHHRYINE
                     RGQRMYFDEPMPVDGFRKHVLESKQTLLISHDYLRMAEELGEEPASAGEAPKSLLFAP
                     MVVDGQVTGIMSLQNLDREHAFQESDTKLLSTIAASLSAALNNARLFEETMRHARESE
                     ALNEVGRDISATLNLSAVLDKIAKHARDLLKGSSSAIYLPEPDGKSMRALVAQGDIAE
                     EILADSIQVGEGIIGSLAKLGKAEYI"
     gene            complement(2066..3235)
                     /locus_tag="PLR93_07635"
     CDS             complement(2066..3235)
                     /locus_tag="PLR93_07635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH26873.1"
                     /translation="MNNPPRILIVDDEPFNVDYLEQELADFGYELLTAANGEEALEKI
                     RTGSPDLVLLDIMMPIMDGFTVLGQAKADAAIRNIPIIIISANNDLQSVVKGLQKGAE
                     DYLPKPFEPTILYARISASLDRKRLRDQEQVYLQSLENELNIARNIQQEFLPAELPVL
                     EGWEIAPYFKAAKFVAGDYYDAFTLPNGNLVFIVGDVCGKGIGAALFMTLFRSLLRAT
                     STAGYLVEETATFPPAKHIEQAIHMTNNYVATTHEQALVFSTVFIGILNPTTGALTYV
                     NAGNEAPFLIRNDGTFEELRPTGPVVGAMPGVAYKVREVLLNSGDSLLAFTDGIPDSK
                     NQQDDFYGHERLVEFIKQKDASATALVNRLGKELDLFIDTEEQFDDITILALRKV"
BASE COUNT          831 a          806 c          786 g          901 t
ORIGIN      
        1 ttgatgtatt ctgccttgcc gagtttcgca agcgacccga taatgccttc cccaacctga
       61 atggaatctg ccagaatttc ctctgcaata tccccttgcg ctaccaacgc ccgcatcgac
      121 ttgccatctg gctcgggcaa ataaatagcg ctgcttgagc ctttcaataa gtcgcgggcg
      181 tgcttggcga tcttatctaa cacggctgag agatttaacg tggcagatat atctcgccca
      241 acttcattga gtgcttctga ttcgcgggca tgccgcatgg tttcttcaaa taagcgggca
      301 ttattcaacg ccgcacttaa gctagcggca atggtagaaa gtaactttgt atccgattct
      361 tgaaaggcgt gttcacggtc caggttttgc aagctcataa tgcctgtcac ctgaccatcc
      421 actaccatgg gagcaaagag cagcgacttt ggtgcttcac ctgcagaagc aggctcttca
      481 ccaagttctt ccgccattct taagtaatcg tggctaataa gcagggtttg tttgctttca
      541 agcacatgct tgcggaaacc atccacgggc atgggttcat caaagtacat gcgttgaccg
      601 cgttcattga tatagcggtg atgcatctca cctgtggcgg gataatacaa tgagatgagt
      661 gtgacttgcg catcaaagat ctcgccgacc ttatcaccga tcaggtcaat gatggattga
      721 aaatccagtt tagagaccag cacttgttgc aggctgttga tgacgcctaa ctcggcattg
      781 cgcccggcag tttcagataa gaggcgctcg gtctcattga acaaacgcga attttcgatg
      841 acgactgcgg cttgatctcc gaacaatttg agtaggcgta attcagactc tgtaaaaata
      901 cgcggttctg caaaatggat attcatcaca cccacaacac ggttgctgaa tttgagcggc
      961 aacccgatca atgcgccacc ccagttggag ttggcgtaga gtgggtcaac gctcatatct
     1021 tctaccaaca tcatttcgcc agatttcgaa acgatatcgg tcataccgcc cgggcgcgga
     1081 tggaaagctg gcgcatttgt aatctttcca tgcgcaatgg ctgtgccaaa ctttaattcg
     1141 ccgttctcat agataaaaag attgatgtcc tcaatgctgg gcatcaatgt ccatgtattt
     1201 tgtagcaagg aattgaggac ggtagtgtaa tctaaacttg aagagagatt gatggtcgct
     1261 ttgcggatgg cttccaactc cgcgttgcgt tgttctgttt cgttgagcag acgttgcgtt
     1321 tcatttaaga ggcgggcatt ttcgagcgca gcacacagag acccggcaag agtctcaagc
     1381 aagcgcacat ctgatttgtt gaaggcgtgc tccttatcca tattttggag ggagattaca
     1441 ccgcgcacct caccggctgt cgtcaacggc acatacaaag ccgactttgg aatcaacgga
     1501 ccgagaacca actctgcgtt gaactctcgc attcggtctt caacatgttc attaaagagg
     1561 aaagtctttt tctcgcggat caaggctttg accaacccgc tcatggggcg gtcttcaata
     1621 tattctcgtc cctgccggtc tacataatag ttgaacgatt gcgtttctgt cttgcgatca
     1681 aaggttgcaa tgatggtggt ttgcgcctta aaaaggtcgc gaatcttttc cccaactaga
     1741 tcataaatcg cctgcatctc caatttggag gcaagcccat cttgcacact tttgataatg
     1801 gcaagctccg cctcacgttg ttccagctcc ttgcggagtt gggtaaccga agtgggtttg
     1861 ctgctggttg ttttcttggt tgtagcgcgg acggcttttt tctttgtggc tgatttgggc
     1921 gcggcgcttt tcttctgacc agttctaggc atggtttcct ctgagaatga ttcagtttac
     1981 atagggggca gagaatcgtc aatagcgtag ataaaaaaac aggcggagaa cgcctgtctg
     2041 tcattcaatt gttatgttca gacaatcata ctttgcgcaa cgccaggatg gtgatatcgt
     2101 cgaattgttc ttcagtatca atgaacaagt ccaactcttt gccaaggcgg tttacaagcg
     2161 ctgttgcaga ggcatccttt tgtttgatga attccaccag gcgttcatga ccgtaaaaat
     2221 catcttgctg gtttttgcta tctggaatgc catcggtgaa agcgagcaag ctatctcctg
     2281 aattaagtaa aacttcacgc actttgtatg ccacacccgg catcgcaccc acaaccggac
     2341 ccgtcgggcg cagttcttca aacgtcccat cgttgcggat taagaacggc gcttcgttcc
     2401 cggcgttgac ataggtcaac gctccagtgg ttgggttcaa gatgccgata aaaactgtcg
     2461 aaaagaccaa ggcttgttca tgcgtggtcg ccacataatt gttggtcatg tgaatggctt
     2521 gttcaatatg tttggcaggg gggaaggtgg cagtttcttc tactaaatac cccgccgtag
     2581 atgtagcacg caacaaacta cggaacaagg tcatgaacaa tgcggctcca atacctttcc
     2641 cacaaacatc tcctacgata aagaccaaat ttccattcgg taatgtaaag gcatcgtagt
     2701 aatcgccagc cacgaacttt gccgctttga agtacggagc gatctcccaa ccctccaaca
     2761 cgggcaactc ggcaggaaga aattcctgct gaatgttgcg cgcaatgttc aactcgtttt
     2821 caaggctttg taaatagact tgttcctggt cccgcaggcg tttcctatcc aaactggcgg
     2881 agatacgtgc atataaaatg gtcggttcga aaggcttggg taaataatct tcggcgcctt
     2941 tttgaagccc cttcaccacg ctttgcagat cgttgttcgc agaaataata atgattggga
     3001 tattacgaat cgccgcatca gctttggctt gccctaatac cgtaaaccca tccatgatgg
     3061 gcatcataat atctagtagt acaaggtctg gagatccagt acgaattttc tccagtgcct
     3121 cctctccgtt tgcagcggta agaagctcat acccaaaatc agcaagctcc tgctctaggt
     3181 aatcaacatt gaatggttca tcatctacga tcaaaatacg cggtggattg ttcataaaag
     3241 aaaggcttat cccaagtatt tggcgagcat gtcaaacagt aaagaatcat tcacgggttt
     3301 tgtgaggtaa tcgttacagc ctgc
//
LOCUS       DAOQFT010000265         3322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16681_length_3322_cov_6.538108, whole genome shotgun sequence.
ACCESSION   DAOQFT010000265 DAOQFT010000000
VERSION     DAOQFT010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16681_length_3322_cov_6.538108"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..458
                     /locus_tag="PLR93_07640"
     CDS             84..458
                     /locus_tag="PLR93_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26874.1"
                     /translation="MAQVSFIPIYSTKGDADAFLLYPYLFNRTGDWIGFVTPTRDVYS
                     VLGEYVGTLTNDPRIVAKRAIDETKPRVAPPPAPKRVQLPANVPLAPMMPELSHSFVD
                     VLIDMPEKLHTIDSGESRQDMD"
     gene            461..922
                     /locus_tag="PLR93_07645"
     CDS             461..922
                     /locus_tag="PLR93_07645"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNH26875.1"
                     /translation="MSKAKTSHASRVYLSQLMQPEHANHHGNVHGGWIMKLVDEAGAL
                     SCMRHAQMRVVTVAVDSLVFREPIKIGDLVTFTAEVTYTGRTSMEAEVQVTAEDPITG
                     ERTHTNTAYLVYVGLDDNNRPAPIPQLVAETEEEKARLELAQQRQKRRIQK"
     gene            928..2469
                     /locus_tag="PLR93_07650"
     CDS             928..2469
                     /locus_tag="PLR93_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26876.1"
                     /translation="MKKISQHPILILLVINLAIGLMTFRSYGLAWDEPLFYDYGQALK
                     YAYTPANWFSGNFDVTQSFGSSGSDHANRGPAYLLVAMPLVSFLEWLGLDLASAWHLV
                     NFLTFNLGIYLLYRLASKWMSKESALAVAALFTFQPLLWGHAFINPKDIPFLTFFLGS
                     VLFGIELIDQWKAKNQYPIPKLLLASFFLGIATSIRILGPLAAILNVLYALVQGIKIS
                     ELIKRPVIWLYACISLVIMFAAWPYLWLNPLDKFVEVFVFMSDNPTQLNVLFAGSNYQ
                     AGEIPLRYFPVLLRYTLTEPTYFLIAIGILLAFWKSDNKRRLTFAILLTWFGILVAYI
                     LLRRPAMYDGYRHFLFILPPLFIFAGFAFEAMFNWLKQGWQKAILTSVILTFGILPII
                     QLHPYQYAYYNNFAGGVGEVFRKYETEYWLTCYREAVLGLDQIAETGAQLFVRREPYI
                     AAYYANSNITIRDFRTEQKDMKSGDYYLVNTRSNEDLKSLRDAPTVLEVSRMGATFCV
                     VKQVP"
     gene            2485..3318
                     /locus_tag="PLR93_07655"
     CDS             2485..3318
                     /locus_tag="PLR93_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNH26877.1"
                     /translation="MTPSPRLLLTIDYEPWFAPSRRYDSWSTERRHDLDANISQQLLD
                     PILEILGASKATFYLVGEMVQWYSSLPEKILHAGHEVGFHCHVHRKLNSIQEIEKDLI
                     ASASWIKQYNVRGYRAPMINTVEEVYPLLERHGFTYSSSIYAPTGVVQKKNNIWEIPA
                     STLPFRKTKTNFHAPRYMDLNLILGGEFPYSSSFMSGLFRRTVFNIIERELKAGKSPV
                     IFLHPYEIITPPNYFQRFAKDLLYYPLLIPFSFNKSQFLKDLLKHFPTSTMQDYIKEL
                     P"
BASE COUNT          852 a          909 c          651 g          910 t
ORIGIN      
        1 ggcattgtga tgttatggaa ctatttcgtc aaccggtact ggacgtataa tgatattgat
       61 gacgacaatt aaggcaggcc gttatggcac aagtatcatt tattccaatc tacagcacta
      121 aaggggacgc agatgcgttc cttttatatc cttatctctt taatcgaaca ggagattgga
      181 tcggcttcgt cacaccaact cgtgatgtat attcggtatt gggtgaatac gttggcacat
      241 taacaaatga tccgcgcatc gtagccaaac gtgccatcga cgaaaccaaa cctcgtgttg
      301 cccctccgcc cgcgcccaaa agagttcaac tgcctgcaaa cgttccgttg gcacctatga
      361 tgccagaact cagtcactcc ttcgtggatg ttcttataga catgccggaa aaactccaca
      421 caattgatag tggcgagtcc cgtcaggata tggattagtc atgtcaaaag caaaaacatc
      481 ccatgcttcg cgtgtgtatc tctcacaact tatgcaaccc gaacatgcca accatcatgg
      541 caatgtccat ggcggttgga tcatgaaact agttgatgaa gccggtgcac tttcttgcat
      601 gcgtcacgcc caaatgcgcg ttgtcaccgt cgccgttgac tcgctcgttt tccgcgaacc
      661 cattaaaatc ggagacctcg tcaccttcac cgccgaagtg acctacaccg ggcgcacctc
      721 catggaagcc gaagtacaag tgaccgcaga agaccccatc acaggcgaac gcactcatac
      781 caataccgcc tatctcgtct atgtcggttt ggatgacaac aatcgccctg cgcccatccc
      841 gcaattggtc gcagaaactg aagaagaaaa agcacgcctc gaattggcac aacaacgtca
      901 aaagagacgt attcaaaagt aatcctcttg aaaaaaataa gtcaacatcc tattttgata
      961 ttgctcgtca tcaatcttgc cattggattg atgacgtttc gttcttatgg cttggcgtgg
     1021 gatgagccgc tcttctacga ttacggtcag gcgctcaaat acgcctacac ccccgcaaat
     1081 tggtttagcg gaaattttga tgtcacccaa tcgtttggct cgagcggcag cgaccatgcc
     1141 aatcgcggac ccgcttatct tttggtagcc atgccgctcg tctctttttt agaatggctt
     1201 ggactcgacc tcgcctcagc ctggcacctg gttaacttcc ttactttcaa ccttggcatt
     1261 tacctactct atcgcctcgc ctcaaaatgg atgagcaaag agtctgcgct tgcagttgcg
     1321 gcgcttttca cctttcagcc tctgctctgg gggcacgcct tcatcaaccc gaaggatatc
     1381 cccttcttaa ctttctttct cggctctgtt ctgtttggca ttgagctgat agaccaatgg
     1441 aaagccaaaa accaataccc aattcctaaa ttattgctcg cctctttctt cctcggaatc
     1501 gctacctcta ttcgtatcct aggtccactc gccgcgattc tgaatgtcct ttatgcactg
     1561 gttcaaggca tcaaaatctc cgaactaata aagcgccctg taatttggct atacgcatgc
     1621 atatctttgg ttatcatgtt cgcagcatgg ccctaccttt ggctgaaccc attggacaaa
     1681 ttcgttgaag tttttgtctt catgtctgat aacccaactc aactaaacgt actctttgct
     1741 ggaagtaatt atcaagctgg tgaaattcca ctgcgttatt ttcccgtctt gttgcgctat
     1801 accctcaccg aaccgactta ttttctaatt gccatcggta ttctgctcgc cttttggaaa
     1861 tcagacaata aaagacgcct gaccttcgcc atcttgttga catggtttgg cattcttgta
     1921 gcgtatattc ttttgcgccg tcccgccatg tacgatggtt atcgtcactt tctttttatt
     1981 cttccaccat tgttcatttt tgctggcttc gcatttgaag ctatgttcaa ctggctaaaa
     2041 caaggctggc aaaaagccat actcacttcc gtcatcttaa cttttggaat tctccccatc
     2101 attcaacttc acccttatca atatgcttac tacaacaact tcgcgggcgg agttggcgaa
     2161 gtcttccgca agtatgagac cgaatactgg ctgacctgtt atcgcgaagc cgttctcggc
     2221 ttggatcaaa tcgcagaaac aggtgcccaa cttttcgtcc gacgcgaacc gtacatcgcg
     2281 gcatattacg cgaattctaa tatcacaatc cgcgatttcc gcaccgagca aaaagacatg
     2341 aagtctggcg attattatct tgtcaacacc cgctcgaacg aagacctcaa gtctttgcgc
     2401 gatgctccca cagtgctcga agtttctcgt atgggcgcga cattttgtgt cgtaaagcaa
     2461 gtcccgtaat ataatccccg cgccatgaca ccttctccgc gcctgctcct caccatcgac
     2521 tacgaaccct ggttcgcacc atcgcgcagg tatgattcat ggagcacaga acgacgccat
     2581 gatttagacg caaacatctc acagcaattg cttgacccca ttcttgaaat cctcggcgca
     2641 tcaaaagcga ctttctatct tgttggcgaa atggtgcaat ggtattcaag cctgccagaa
     2701 aaaattctgc atgcagggca tgaagttggt tttcattgcc atgtccatcg aaagctcaac
     2761 agcattcaag aaattgaaaa agaccttatc gcctctgcca gctggatcaa gcaatataac
     2821 gtccgcgggt atcgtgcgcc gatgatcaac acagttgaag aagtttaccc acttcttgaa
     2881 agacatggct ttacctacag ttcttcaatt tatgcgccca cgggcgtagt gcaaaaaaag
     2941 aacaacatat gggaaatccc tgccagcact ctcccattcc gtaaaacaaa aactaatttt
     3001 cacgcaccac gctatatgga cctcaacctc atcctcggcg gcgaattccc ttatagctcc
     3061 agcttcatga gcggactttt ccgccgtact gttttcaaca tcattgaacg tgaactcaaa
     3121 gcaggcaaaa gtcctgtcat ctttttgcat ccgtatgaaa ttatcacgcc gccaaattat
     3181 tttcagcgtt ttgctaaaga cctgctttat tatccattgc taattccgtt tagcttcaac
     3241 aaatctcaat tcctcaaaga cctactcaag catttcccaa cctccactat gcaagattac
     3301 atcaaagagc ttccatgaac at
//
LOCUS       DAOQFT010000266         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16821_length_3306_cov_6.557059, whole genome shotgun sequence.
ACCESSION   DAOQFT010000266 DAOQFT010000000
VERSION     DAOQFT010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16821_length_3306_cov_6.557059"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLR93_07660"
     CDS             <1..478
                     /locus_tag="PLR93_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH26878.1"
                     /translation="AGFDVIEGDRPGIPDWILEKSKETQEDAMKSIRKAYEMGVPIAM
                     GTDAATPYNFHGENAMELYYMSQAGISAMDCIVASTKNAAHALGWDSKLGTLKAGKLA
                     DLIVVKKNPLDDLRSLANKNNIEYVMQGGKFVARQFVEPTGIPEELMAGAWICCGF"
     gene            498..1823
                     /locus_tag="PLR93_07665"
     CDS             498..1823
                     /locus_tag="PLR93_07665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNH26879.1"
                     /translation="MKRVYQYLSVYVFLALACSSLTPSTSTGTGYRYDNHPDANTQSA
                     EYRALSKWGKTDITYYFINGTDKISGDREQELVAQAFALWAAQTSLTFTETNSRQSAD
                     IVIGWAEGEHGDGDPFDGPGDVLAHASYPNPYEERQVFLHFDDAERWVDSDTQDVDLL
                     TVAAHEIGHALGLGHSNDPNSLMYPSYSEPHRFLGGDDIAGIQSLYGLASEPPTAPEV
                     PKPNENPPTSPSQDSDGDGLADSDETLITGTDPNNKDSDSDGIGDGVEVVNRMNPLDA
                     DMDKDGVKDGAEIAAGTNPFLPDQANGVSPELSKEVGEFLTKAIKLQIEAYRQGDASV
                     ASVIMAGDVLQSLQGEISSLNQQGLVEIADIDYYKSYIDDIRIVNNAQLEVDTCEVWS
                     LQYYRRSDGALVQSNEPTLLPQTITIQKIENGWFITAVQFFEAPAFCHS"
     gene            2026..>3306
                     /locus_tag="PLR93_07670"
     CDS             2026..>3306
                     /locus_tag="PLR93_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26880.1"
                     /translation="MFVTKNLYQNDEAWKNVPLGFSSQTIGSWGCLLTSVTMMLNGIG
                     YNETPDTVNTKMKNAGGFQGPLFIPSVLPYVWGNCAYRDMQPCESVPAPLQQIDDAVA
                     SGKPVILQVDWNKQAGIQTHFVLVKEKKGDDYVLYDPYKYGGDGPDKEVLLTTRYKYN
                     GAKLSTEISAVLWFENYGINPPEPPAKVPVPAENFILYAAEDDLALRSQPATTGYLWK
                     RMVMNTELICLEPKAQAAAKIGVQGQWIQVQDPKGDQGYVAGWFVSDKKAVPAPTAPV
                     TPASPSTSKPGTAPKPSSSASKPGSAPAPTPSKPAPVPPGALAFFPTEEVSFRSQPVV
                     ADNTLIRKIPVTEQLVSTEPADKVIPKVGVQNQWLKVRAADNKEGYVAAWYVKYAGGS
                     TAQANAAAAPAPAASTGGAIKVRTVAEAVSLRKQP"
BASE COUNT          873 a          828 c          826 g          779 t
ORIGIN      
        1 agccggtttt gacgtaattg aaggagaccg acctggcatc cctgattgga ttttggaaaa
       61 aagcaaagaa acacaggaag atgcgatgaa atccattcgc aaggcatatg aaatgggagt
      121 acctattgcg atgggtacgg atgctgccac cccatataat tttcatggcg agaatgcgat
      181 ggagctatat tacatgtcgc aggcgggaat ctctgcaatg gactgtattg ttgcttctac
      241 gaagaatgcc gctcacgcac tcggctggga ctcaaagctt ggcacgctga aagccggtaa
      301 attagccgat ctgatcgtgg tcaaaaagaa tccgttagat gacctgcgtt ctttagccaa
      361 taaaaataac attgaatatg tcatgcaagg cggtaaattt gtggcgcgcc aattcgtaga
      421 accgaccggc attcctgaag aattgatggc tggcgcttgg atctgctgcg gattttagtt
      481 ttattggagg ttaaaaaatg aaacgcgttt atcaatatct gtctgtatat gtttttctcg
      541 cacttgcctg ttccagcctt acaccgagta catccactgg aacaggttat cgatacgata
      601 atcatccgga tgccaataca cagtcggctg aatatcgcgc actttccaaa tggggcaaaa
      661 ccgatatcac ctattatttc atcaacggca ccgacaaaat ttcaggagac cgagagcaag
      721 aattggtggc tcaggcattt gcactatggg cagcgcaaac ttcactcact ttcacagaga
      781 ccaactcaag acaaagcgcg gatattgtca ttggctgggc agaaggtgaa cacggagatg
      841 gcgacccatt tgacggacct ggcgatgtac tagcacacgc ctcttacccc aacccgtatg
      901 aggagcgcca agtcttttta cattttgacg acgccgaacg ttgggtggat agcgataccc
      961 aagatgtaga cctgcttacc gtagcagcac atgagatcgg tcatgcgttg ggactgggtc
     1021 acagcaacga cccgaactct ctgatgtatc catcctatag tgagccgcat cgttttctag
     1081 gcggggatga tattgcgggc attcaatcac tttatgggtt ggcaagtgaa ccacctaccg
     1141 caccagaagt gcccaagccg aatgaaaacc cgcccaccag cccaagccag gactctgatg
     1201 gagatgggct ggcagactca gacgaaactc tcatcacagg tacagacccc aataataaag
     1261 attcagatag tgatggcatt ggcgatggcg tagaagtggt caaccgcatg aatccgcttg
     1321 atgctgatat ggataaagac ggagtgaaag acggcgcgga aattgcggcg ggaaccaatc
     1381 ctttcctgcc agaccaagcc aatggagtct cgccggagct ttcgaaggaa gtcggcgaat
     1441 ttctcacgaa ggcgatcaag ctgcaaattg aagcctatcg ccaaggcgat gcttctgttg
     1501 cgtctgtgat tatggctggc gacgtactac agtctctaca gggagagatt tcctcgctca
     1561 accaacaggg gttggtcgag atcgcagaca ttgactatta caaaagctat atcgacgata
     1621 ttcgaatcgt caacaatgct cagcttgaag tggatacttg tgaagtctgg tctttgcagt
     1681 attatcgccg ttcagatggt gcccttgttc aaagcaatga gcctacgcta ttgccacaaa
     1741 cgatcactat tcaaaagata gagaatggct ggtttatcac tgcggttcaa ttttttgagg
     1801 caccggcttt ctgccattcg taaaatgcgc cccattggca ggtcgattta tgagggaaaa
     1861 aactgccaat ggggtgagcc aagaggcggc ggagagagag ccgggcggca tccaccttaa
     1921 aatattgcaa aatcgttgca aaggcaaatc gagtagaatt agataaattt atccttaaaa
     1981 agcaagattg aaattcccaa tctccaaatg gaggaggcaa tttttatgtt tgttacgaag
     2041 aacttgtacc aaaatgatga agcctggaag aacgttccgc tcggtttttc atcgcagacc
     2101 atcggttctt ggggttgttt gctcacttca gtgactatga tgttgaacgg catcgggtac
     2161 aacgaaacgc ccgatacggt caacactaag atgaagaacg cgggtggttt tcagggtccg
     2221 ctcttcattc ccagtgtgtt gccttacgtt tggggaaact gcgcataccg cgatatgcag
     2281 ccttgtgaat ctgttccggc tccgctgcaa cagatcgacg atgcggttgc ttctggcaaa
     2341 cccgtcattt tgcaagtgga ctggaacaaa caagctggta ttcaaactca cttcgtcctt
     2401 gtgaaagaaa agaagggcga tgattatgtg ctttatgacc cgtataaata cggcggcgac
     2461 ggtccagata aagaagtatt gctaaccacc cgctacaaat acaacggtgc caaactctcc
     2521 accgagatta gtgctgtatt atggttcgaa aactacggca tcaacccgcc tgaaccacct
     2581 gccaaggttc ccgttcctgc tgagaatttc atcctatatg ccgccgaaga tgatttggct
     2641 ctgcgttctc aacccgccac cactggctac ctgtggaagc gcatggtgat gaatactgag
     2701 ctcatctgcc ttgaacccaa agcccaagcc gccgccaaga tcggcgtgca aggtcaatgg
     2761 attcaggtac aagacccgaa aggcgatcag ggatatgtgg ctggctggtt cgtttctgac
     2821 aaaaaggctg tcccagctcc gactgctcct gtaacgcccg cctctccttc gacgtcgaag
     2881 ccgggtacgg ctcccaagcc ttcttcttcc gcctcgaaac ccggttcggc tcctgcccca
     2941 accccaagca aacctgcgcc tgttcctcct ggcgccttgg cattcttccc caccgaagaa
     3001 gtctccttcc gatctcagcc tgtcgttgca gataacacct tgatccgcaa gatccctgtg
     3061 accgaacaac tcgtcagcac agagcctgcc gataaggtca ttccgaaagt cggcgtgcaa
     3121 aatcaatggc tcaaggtgcg tgccgctgat aacaaagaag ggtatgtagc agcttggtac
     3181 gtgaagtatg ccggtggttc cacagctcag gcaaatgctg cagccgctcc tgcccctgcc
     3241 gcttcaactg gtggcgcgat taaagtccgc accgtggcag aagccgtctc tttgcgcaaa
     3301 cagccc
//
LOCUS       DAOQFT010000267         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16833_length_3305_cov_6.220923, whole genome shotgun sequence.
ACCESSION   DAOQFT010000267 DAOQFT010000000
VERSION     DAOQFT010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16833_length_3305_cov_6.220923"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PLR93_07675"
     CDS             <1..1173
                     /locus_tag="PLR93_07675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNH26881.1"
                     /translation="TLTLDSVLHPKGRGYPKSRADGRVIQGDNLAVMAALLPEYEGRI
                     NLIYADPPFFTNRQFHARVGRGEDSRKPSKWKLAEGYQDSWTDMDSYLQFLYERLSLM
                     HRLLAKNGTLYLHLDWHADSHARLILDEIFGAENFINEIIWAYHGPSPIRTAFNRKHD
                     TILMYAKSKEYIFNVDDVREPYNPNTVATFKASRKAGFGKIPDLERGKVPEDWWYFPV
                     VARLHNERTGYPTQKPEALLERIILASSNKNDLVADFFCGSGTTALVAAKHGRKFITS
                     DQSARAVHTARARLAETKSVFSMERDVMVKTEIATKSKKIKVKLMGDRVLLQTSLDVD
                     FWEVDPAWDGKIFRSAVQAQRHVRSGEIPMELKIKLGGKVCIRVVTVEGKQFQLNI"
     gene            complement(1162..1818)
                     /locus_tag="PLR93_07680"
     CDS             complement(1162..1818)
                     /locus_tag="PLR93_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26882.1"
                     /translation="MKTTTLLVIEAKHAEVPSFAADLQKKGFDVHIAQNGSKAVALLK
                     EVSPSLVVVNAASMRSTGLRICQSLREKDSKLPIILILDEEKEVSKDAADAVLILPFT
                     VQKLSNRIKPLLPGDGKNVLHVGPIRLDLEKRRVRCLGKSTKLTPRLVTLLQLLMEKH
                     GEVVEREPLFKKAWETNYTGDTRTLDVHISWLRRAIELDPDNPKFLKTVRGVGYRLDI
                     "
     gene            complement(1990..3066)
                     /gene="rlmN"
                     /locus_tag="PLR93_07685"
     CDS             complement(1990..3066)
                     /gene="rlmN"
                     /locus_tag="PLR93_07685"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNH26883.1"
                     /translation="MLIYDLNLPALESVLKEWNEPAYRAKQIWQGLYQHLYSSPEQFS
                     NLPNSLRSKMAEHFTFNPFTIKTYLDSSDKSTRKTLFQLPDGNLIESVLMRYGDPADE
                     HSTSSEKASKQRRTLCISTQAGCAMGCVFCATGQLGFKRNLSSGEIVAQVMYYAQMLK
                     GQDEVVSNVVFMGMGEPFHNYDNVMSAIDRLNDADGYKFGARRLTISTVGLVPQIRRF
                     ADEQRQVNLAISLHAADDDERLAIMPVNKRYKIDDVIDACKYYIEKTHRRVTFEWALI
                     NGVNDTPEVAQKLASRLKGLLCHVNAIPLNPTQGYQGEATSRQRAQVFRETLEQAGIP
                     CTIRMRRGIDINAGCGQLAGAESK"
BASE COUNT          901 a          758 c          750 g          896 t
ORIGIN      
        1 acattaaccc ttgactctgt cctccacccg aaaggacgtg gctaccccaa atcgcgtgcc
       61 gatggacgag tcattcaagg ggataatttg gcagtgatgg ctgctttgtt gcctgaatat
      121 gaaggacgca tcaacctcat ttacgccgac ccaccatttt tcactaacag acaattccat
      181 gcccgcgtgg gcaggggaga agactctcgc aaaccttcca agtggaaact tgctgaaggc
      241 tatcaagatt catggacaga tatggactcg tacctgcaat ttctgtacga acgtctcagt
      301 ctcatgcatc gactgctcgc caaaaacggc acgttatatc ttcaccttga ctggcacgct
      361 gattcccatg cccgcctcat cctcgacgaa atttttggcg cagagaactt catcaatgaa
      421 attatctggg catatcatgg accttctcca atacgaaccg catttaatcg aaaacatgac
      481 accatcttga tgtatgctaa aagtaaagaa tatattttca atgtagatga tgtccgcgaa
      541 ccgtataacc ccaataccgt agcaaccttc aaggcatctc gcaaggcagg ttttggcaag
      601 attcctgatc ttgaacgtgg caaagtgcca gaagattggt ggtatttccc cgtggtggca
      661 cgcttgcaca atgaacgcac ggggtatccc actcaaaagc cagaagcttt gctcgaacgc
      721 attattttgg catcttcgaa taaaaatgac ctagtagcag actttttctg tggttctggt
      781 accaccgctc ttgtggcagc caagcatgga cgcaaattta tcacctccga ccagtctgct
      841 cgagctgtgc ataccgcgcg cgcacgattg gctgagacca agtctgtgtt ctccatggaa
      901 cgtgatgtga tggtcaaaac agaaattgcg accaaatcaa agaagatcaa ggtcaagttg
      961 atgggcgacc gagtcctttt gcaaacatct ttggatgtgg acttctggga agtggaccca
     1021 gcttgggatg ggaaaatttt ccgcagcgca gtgcaggctc aacgtcacgt gcgaagcggg
     1081 gaaattccga tggagttaaa aataaaactc ggaggcaaag tctgcatccg agtcgtaacg
     1141 gttgaaggaa agcaatttca actaaatatc taagcgatac ccaacaccgc gcacggtctt
     1201 gagaaatttt gggttatcgg ggtccagctc aatggcgcgt cgcaaccatg agatatgtac
     1261 atcaagggtg cgggtatcac cggtgtaatt ggtttcccat gcttttttga agagcggttc
     1321 acgttccaca acttctccat gtttttccat caacaactga agcaaagtta ccaaacgcgg
     1381 ggtgagtttt gtgctcttgc ccaaacagcg cacacgtcgt ttttcaaggt ctaaccgaat
     1441 aggacccaca tgcaaaacgt tttttccgtc accgggcaat aaaggcttga tgcgattgga
     1501 aagtttttga actgtgaacg gcaaaatcaa aactgcatcg gctgcatctt tgctaacttc
     1561 tttttcttca tccagaataa gaataatggg gagctttgaa tctttttcgc gcagcgactg
     1621 acagatgcgt aaaccagtac ttcgcatgga tgccgcattg acaacaacca agcttgggct
     1681 gacttctttc aacaacgcaa cagctttgct gccattctgt gcgatatgta catcaaatcc
     1741 cttcttctgc aagtctgctg cgaaagaagg gacttctgcg tgtttggctt caatgaccaa
     1801 tagggttgtg gtcttcataa gaaatttcga taatgccttt ttgtgaattc gtactgcaat
     1861 aggcttttaa gcttgttaag attgattcat tatacaccat atcttaacaa agggtctcaa
     1921 acctgagtac tatttgcatc ttaatttcat tgacatattt tttaagttgt ggaatcaaat
     1981 cagaaaattt tattttgact cagcgcctgc aagttgacca cagcccgcat tgatatcaat
     2041 accgcggcgc atgcggatcg tgcatggaat tccggcttgt tccaatgttt ctctgaatac
     2101 ttgcgcacgc tgacggctcg tcgcttcgcc ttgatagcct tgtgtggggt ttaatggaat
     2161 ggcattcaca tgacaaagca agcctttgag tctggaggcg agcttttgcg ccacttccgg
     2221 cgtatcgttg actccattaa tgagcgccca ttcaaaggtc acgcgacgat gcgttttttc
     2281 aatatagtat ttgcaggcgt cgataacatc atcaatctta taacgtttgt tgaccggcat
     2341 aatcgcgaga cgctcatcat catcggcggc gtgaagggaa atggctaagt tgacttggcg
     2401 ctgttcatct gcaaagcgcc gaatttgcgg cacaagccct actgtggaaa tggtcaagcg
     2461 gcgtgccccg aatttatatc cgtcggcatc gttcaaacga tcaatggcag acatcacatt
     2521 atcgtagttg tgaaaaggct cgcccatgcc cataaaaacg acgttgctta caacttcgtc
     2581 ttgtcctttg agcatttgcg cgtaatacat gacctgtgcc acgatctcac cgctggaaag
     2641 attgcgcttg aacccgagct gacctgtggc gcagaacaca catcccatag cgcagccagc
     2701 ctgcgtggag atgcaaagcg tgcgcctttg ctttgaggct ttttcagaag aagtggaatg
     2761 ctcatcggcg gggtcgccat aacgcatcaa aacagactcg atcaaatttc catcaggcag
     2821 ttgaaataac gttttacggg tagatttatc agatgagtct aaataggttt taattgtgaa
     2881 tggattaaag gtaaaatgct cagccatttt gctgcgcagg gaattcggca gattgctgaa
     2941 ttgttctggt gaagaataaa gatgttgata cagtccctgc cagatttgct tggcgcggta
     3001 tgcaggttca ttccattctt ttaagacaga ttcaagagcg ggaaggttaa gatcgtaaat
     3061 taacataaat ggtttcctat tttacaccag caaatggaaa tcccattttt cgtaggaatg
     3121 gaacttatct ctcatggaga ccgtcataaa tctataaaat tctgcccgta tcgtacaaga
     3181 aaagtaggaa attcgtaggg aaatacctat gttttcttaa ttttttccaa atataattct
     3241 aagccttgct tgaaaacgaa ggagaaattt atgttgaaac caatgaaacc aaagcaaact
     3301 tttcc
//
LOCUS       DAOQFT010000268         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_16966_length_3288_cov_6.632230, whole genome shotgun sequence.
ACCESSION   DAOQFT010000268 DAOQFT010000000
VERSION     DAOQFT010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16966_length_3288_cov_6.632230"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PLR93_07690"
     CDS             <1..1101
                     /locus_tag="PLR93_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH26884.1"
                     /translation="MTTKIRALASREVASVTPQGKLSKVLFTLGGADANENAIKLARG
                     YTKRHKILTRYRSYHGASAGAMAATGDPRRVAWEPNLMTGVVHFLDPYRYRSTFHRTN
                     PNISEADFAKDYVNHLEEIILYEGPESIAGILMESVTGTNGIIIPPDGYMQGVRTLCD
                     KYGIVMICDEVMSGFGRTGKWFAIENWNVVPDMITMAKGLTSAYAPLGAVAMKPEIAA
                     AFDEHAFESGLTYTSHPISLAAAVANIQAMREDKVVEHAASMGGVLRRMLSDLGEAHP
                     SVGEVRNIGLFGIIELVKNRETKEPMVPWNGSSPEMVALKKYCSDHGLFLYTHWHTVL
                     IIPPLIINEKELQEGFDVLDEALKITDRTSDG"
     gene            1154..1465
                     /locus_tag="PLR93_07695"
     CDS             1154..1465
                     /locus_tag="PLR93_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26885.1"
                     /translation="MESQPFNSSPAPAPASGGNERVMAIASLVIGVINLCAWFLPICG
                     APLGIIGIVLGIFGMKDPSQKTLAIIGIALSAIGLLAACINGAVGAYMGVTGQGFNFS
                     P"
     gene            complement(1516..>3288)
                     /locus_tag="PLR93_07700"
     CDS             complement(1516..>3288)
                     /locus_tag="PLR93_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH26886.1"
                     /translation="AGYLKELHKRGEKLPHPIVAKLMKSIASAMDHAHERNIVHRDIK
                     PANILLRSKQGVIDLQAPLPEDVEPVLTDFGLVRLLDSNTKTSTGTVSGTPSYMSPEQ
                     ARGDSVDHRTDIYSLGVVLYEMLAGEVPFDAESSFGVLMKHLNEPPPPINGISAGMQA
                     IINRALAKDPKDRFASAIEMVNEFADVLEGKSVSKETLRINSLPKETTANAKQATSSF
                     KTPLLVGGGIIALALIVSLGVFIFRQPRETNVPSQNNNQLPNPSQPIGSVVFDDYNEL
                     VDNATVTISGIPLPPAGQHFEVWFLGQGGEIKLNAGPVIFTGQNGQLIFSDSNGANLL
                     SQFDEMMVTVEPDNDPNPDQPSQEVAASSVFPPQAFVHIRHILVSIGMTPNGTALIQG
                     VWDTADTIDTSVLELRDAFNEGDEATLRIKNEEIINQLVGEENLQLYKDWNGDGEVND
                     PNDGFGLLANGTEQGYIPLTISHTEFAIDAPDATTGIIQSGQNLIVCLQNMEGWSEQL
                     LEVALKLQSMPYGTGMESTIAEIETYSSLLLYGEDTNGNNRIEPLPGEAGADTAYFAA
                     YSIAQMPIFPGGRRMPQPANTTQP"
BASE COUNT          789 a          681 c          879 g          939 t
ORIGIN      
        1 atgacgacga aaatccgcgc cttggcgagt agagaagtgg catcggtcac accgcaggga
       61 aaattgagca aggtgctgtt cacgctcggt ggtgccgatg cgaacgagaa tgccatcaaa
      121 ttggcgcgag gatacaccaa acgtcataag atattaacgc gctatcgttc gtaccatggt
      181 gcttctgctg gcgctatggc tgccacagga gatccgcgcc gggtggcttg ggagccgaat
      241 ttgatgacag gcgtggttca tttccttgac ccatatcgat atcgatccac ctttcaccgt
      301 accaacccga atatcagtga agcagatttt gcgaaggatt atgtcaacca tttagaagaa
      361 atcattctgt acgaaggtcc cgagtctatt gcaggcatct taatggagtc tgtgacaggc
      421 acgaacggta tcatcattcc gccagatgga tacatgcagg gagttcgtac gttatgcgat
      481 aaatacggta ttgtgatgat ctgcgatgaa gtgatgagtg gcttcgggcg cactggcaaa
      541 tggtttgcaa ttgaaaattg gaatgtggtg ccagatatga ttactatggc aaaggggctt
      601 acatcggcat atgctccgct cggggctgtg gcaatgaagc cagagattgc tgctgcgttt
      661 gacgagcatg cctttgaaag cgggctgacg tatacatctc atcctatttc attggcggca
      721 gctgtggcga acatccaagc gatgagggag gataaggttg tagaacacgc agcaagcatg
      781 ggcggagtct tgaggcgtat gttgtccgat ttgggtgagg cgcatccatc tgtgggtgag
      841 gtgcggaata ttggtctatt tggtatcatc gagttggtaa agaatcgtga gacgaaagaa
      901 cctatggtgc catggaatgg gtcttcgcca gaaatggttg ccttgaaaaa atattgttcc
      961 gatcatggct tgtttctata tacccattgg catacggttt tgatcatccc gccgttgatt
     1021 attaacgaaa aagaattgca ggaaggcttt gatgttttgg atgaggcatt gaagattaca
     1081 gataggactt cggatggata acttcatacc gagtttgaag taaaatttta gaaatcaaaa
     1141 aggagaatgt ctaatggaat cccagccctt caactcttcc cctgctcccg cccctgcttc
     1201 tggtgggaat gaacgtgtga tggctatcgc ttcactcgtg attggcgtca ttaatttgtg
     1261 cgcatggttt ttgcctatct gtggcgcacc acttggcata atcggtattg tgcttggtat
     1321 cttcggtatg aaggatcctt cacagaagac cttggcgatc attggtattg ccttaagcgc
     1381 aattggtttg cttgcggctt gtatcaatgg tgctgtaggt gcttatatgg gcgttacggg
     1441 gcaaggtttt aatttctctc cgtaattctt tatcaggcgt tgaaaaaagc tcttgcaata
     1501 tgcaagagct tttttttatg gctgtgttgt attggcgggt tgtggcatgc gcctgccgcc
     1561 tgggaagatg ggcatttggg caatagaata tgcggcaaag taagcggtat cggctccggc
     1621 ttcaccaggg agcggttcaa tacgattgtt accatttgta tcttcgccat atagtaagag
     1681 gcttgaatag gtttctattt ctgcaatggt tgattccata ccagtgccat agggcatact
     1741 ctgaagttta agcgctactt caagtagttg ttcgctccag ccttccatat tttgcagaca
     1801 aacaattaag ttttgtccgc tttggatgat gcctgtggtt gcatcgggag catctatggc
     1861 gaattctgta tggctgatcg tcaaggggat gtagccttgt tctgtgccat ttgcaagtag
     1921 cccaaaccca tcattggggt cgttgacctc accatctcca ttccagtctt tatacaactg
     1981 aaggttttct tctcctacaa gttgattaat aatttcttca ttctttattc gcagagttgc
     2041 ttcatcgcct tcattgaatg catctctaag ctctaaaaca gatgtatcga ttgtatcggc
     2101 tgtgtcccat acgccttgga tgagggctgt tccatttggc gtcatgccta tggagacaag
     2161 aatatgcctt atatggacga atgcctgcgg agggaacacc gaagaggcgg caacttcctg
     2221 gctgggttgg tcggggttgg gatcattatc cggctctacc gtgaccatca tctcatcaaa
     2281 ctgactgaga agattcgcgc cgtttgagtc tgagaaaatt aattgtccat tttgcccggt
     2341 aaatatcacc ggacccgcat ttagttttat ttctccgcct tgacccagga accagacttc
     2401 aaaatgttgc ccggctggcg gtagggggat tccgctgatg gtaactgtgg cattatcgac
     2461 caattcattg taatcatcaa agacaacgga gccaatgggt tgacttggat tgggtaattg
     2521 attgttattt tgggatggta cgtttgtttc acgcggctgg cggaagataa acacgccgag
     2581 tgaaacgatt agggcaaggg caatgatccc tccgccgacc aataggggag tcttgaacga
     2641 tgaagtggct tgttttgcat ttgccgttgt ttcttttggc aaactattga ttcttaaggt
     2701 ctcttttgat acagatttac cttcaaggac atcggcaaat tcattgacca tttcaatcgc
     2761 ggaagcaaaa cggtctttgg ggtcttttgc aagtgcgcga ttgataatgg cttgcatacc
     2821 cgctgaaata ccattaatcg gtgggggagg ctcatttagg tgcttcatta gaacaccaaa
     2881 tgaagactcc gcgtcgaagg ggacttcccc ggcaagcatt tcgtaaagaa caacacctag
     2941 agagtagata tctgttcggt gatcaacact atctccacgg gcttgttcag ggctcatgta
     3001 agaaggggtt ccggagacgg taccggttga ggtcttcgta ttcgagtcga ggaggcggac
     3061 caagccaaag tcggttagaa ccggttctac atcctctgga aggggagctt gtaaatcgat
     3121 gacaccttgt ttggaccgaa gcagaatatt ggcaggtttg atatcgcggt gcacaatgtt
     3181 gcgttcatgt gcatggtcca tggctgaggc gatggatttc atgagtttgg caactatggg
     3241 gtgtggaagc ttttcgccgc gcttgtgcaa ttcttttaga tagccagc
//
LOCUS       DAOQFT010000269         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17070_length_3274_cov_6.090401, whole genome shotgun sequence.
ACCESSION   DAOQFT010000269 DAOQFT010000000
VERSION     DAOQFT010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17070_length_3274_cov_6.090401"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PLR93_07705"
     CDS             complement(<1..1096)
                     /locus_tag="PLR93_07705"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HNH26887.1"
                     /translation="MILHEKMSAQLPAWRERIKSLAKEHGEVVVDKVTVGQIVGGMRD
                     IKSLLTDVSFVDPAEGIRFRGMSIPEVLKALPKAKGGKMPLVGGLFYLLLIGEVPTKE
                     QALEVEAEWAKRANVPDYVFKMLKTMPKETHPMTLFSQAVLALQNESVFAAKYHSMKK
                     DQYWEAALEDSLDLTAKLPIIAAYIYRMKFFGETSKPKYNPKQDYGLNFARMMKVSDK
                     KGYADLARLYFILHSDHESGNVSAHTMHLVGSALSDPYLAFSAALNGLAGPLHGLANQ
                     ECLGWLLSVKEKFGGVPSRDDLYKFSWDTLNGGQVIPGYGHAVLRVPDPRFTAQMEFA
                     KKRFPDDELVRLADMVFDVVPAVLKEQGKAK"
     gene            complement(1168..2103)
                     /gene="mdh"
                     /locus_tag="PLR93_07710"
     CDS             complement(1168..2103)
                     /gene="mdh"
                     /locus_tag="PLR93_07710"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNH26888.1"
                     /translation="MKKISIIGAGNTGATAAHWIAERELADVVLLDVVEGMPQGKSLD
                     LLEAMPIIGKDSHVLGTNNYEDTKNSDIIIITAGIARKPGMSREDLLKTNAEIVGKAA
                     TETLKHSPNAIFIVLTNPLDTMAYLVMKKTGLPRERVIGQAGILDSARMRAFVAMEAN
                     VSVENVQCYVLGGHGDEMVPLTRHSNIAGVPLKEYFPADKLEAIVNRTRKGGGEIVNL
                     LKTGSAYYAPSMACVQMAEAILKDKKLIVPCATLMQGEYGLNDMYFGVPVMLGAGGME
                     KVIEYKFDADEKAMFEKSAASVRESHDALKSVVSL"
     gene            complement(2336..>3274)
                     /gene="trxB"
                     /locus_tag="PLR93_07715"
     CDS             complement(2336..>3274)
                     /gene="trxB"
                     /locus_tag="PLR93_07715"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNH26889.1"
                     /translation="NRHVKVLILGAGPAGLSAALYAARAELAPVVLTGMQLGGQAALT
                     HTIENYPGFPEGVGGPQLGELFQKQAEHFGAKVEFDMAHEVDFSQRPFKVTADSGDYY
                     ADSVILATGANPVHLNVPGEYEMTGRGVSYCATCDGWFFKDKKVVIVGGGDSAFEEAL
                     FITRYASTVTLIHRREEFRAGAILQRRAKEHPKMNFILNTVVTEVNQGDKLTTLKLKN
                     VQTNEESTFDTDGLFIFIGHTPNTDMFKGKLEMNELGYLKINDKMETSIPGVFAAGEV
                     ADATFKQVVTSAGMGAAAAIQATRYLESLEEEAAHS"
BASE COUNT          809 a          848 c          806 g          811 t
ORIGIN      
        1 ttttggcttt gccttgttcc ttcaacacag cagggaccac gtcgaagacc atatcggcaa
       61 ggcgcaccaa ttcatcatca gggaagcgtt ttttggcaaa ttccatctgc gccgtaaagc
      121 gtgggtcagg tacacgcaac acggcgtgac catagccggg gatgacctgt ccaccattga
      181 gggtatccca agagaacttg tagaggtcgt cgcgagaagg gacaccgcca aatttctctt
      241 tcacactgag caaccagccc aggcattcct gattggcaag cccatggagc ggacctgcca
      301 aaccattgag agcggcagaa aaggcgaggt aaggatcaga gagggcagac ccaaccaaat
      361 gcatggtatg agccgaaacg tttccagact cgtgatcact gtgcaagatg aagtacaagc
      421 gggcaaggtc ggcatagcct ttcttatccg aaaccttcat catgcgagcg aagttcaagc
      481 cataatcttg tttgggattg tatttcggct tgctggtttc gccgaagaat ttcatgcggt
      541 atatatacgc cgcaatgata ggaagcttcg ccgtaaggtc aaggctatct tcgagggcgg
      601 cttcccaata ttgatctttc ttcatactgt gatacttcgc cgcaaagacc gactcattct
      661 gcaaagcgag caccgcttgc gagaagagcg tcatggggtg agtctctttc ggcatggtct
      721 tcaacatctt gaagacataa tcaggcacgt tcgcgcgttt cgcccattct gcttccactt
      781 caagggcttg ttccttggta ggcacttcgc cgatcaacag aaggtagaac aagccaccca
      841 ctaaaggcat cttgccgccc tttgccttgg gcagcgcctt tagcacttct ggaatagaca
      901 taccgcggaa gcgaatgcct tcagccgggt ctacaaaaga gacatcggta agcagggatt
      961 tgatatcgcg cataccgcca acgatctgac cgacggtaac tttatcaacc acaacttcgc
     1021 cgtgttcttt agcaaggctc ttaatgcgtt cgcgccaggc aggcaattgg gcagacattt
     1081 tttcgtgcaa aatcatggga gtctcctatt gattttcaat acaaaagtcg aaagccattc
     1141 tgaccttcga cttttgacct ttgatggtta tagactcaca acgctcttga gggcgtcgtg
     1201 tgattctcga acagaagcag ccgatttctc gaacattgcc ttttcgtcgg catcaaactt
     1261 gtattcgatc actttttcca tgccgcctgc acccaacatg acgggcacac cgaagtacat
     1321 atcgttcaaa ccatattcac cctgcatcaa cgtagcgcaa gggacgatga gtttcttatc
     1381 cttcaaaatg gcttctgcca tctgaacaca agccatggaa ggcgcataat aggcagaacc
     1441 cgtcttgagc aagttcacga tctcgccgcc gcccttgcgg gtgcggttga caatagcttc
     1501 aagtttatca gcagggaaat attccttgag cggaacgcct gcaatgttgg agtgacgagt
     1561 aagcggaacc atttcgtcgc cgtgcccacc caacacatag cattgaacat tttcaacaga
     1621 aacattggct tccatcgcaa cgaaagcacg catacgggca gagtccaaaa tgcccgcctg
     1681 cccaatgaca cgttcgcgcg gtaaaccagt tttcttcatg accaagtacg ccatcgtatc
     1741 aagcgggttg gtcagcacaa taaaaatagc attcggagaa tgcttaaggg tttcagtagc
     1801 agccttgccc acgatctcgg cattggtctt gagcaagtct tcgcggctca tgccgggctt
     1861 gcgagcaata ccagcggtga taataatgat gtctgaattc tttgtgtctt cgtaattgtt
     1921 ggttcccaac acatgcgagt ctttcccaat aatcggcatg gcttcgagca ggtccaggct
     1981 ttttccttgc ggcattccct ctacaacatc tagcaaaacg acatcggcga gttcacgttc
     2041 ggcgatccaa tgcgcagcgg tggcgccggt attgcccgcg ccaataattg agattttctt
     2101 catcgtactt cctccaatgg gatcggctaa aaagccgtat ttttttataa ttttatccga
     2161 aagtgaccta gaccaaaagt acaaaatgtc acatttttgg acaaatccac tcaagaaaac
     2221 agactcgccg ctaaaagcga gtctgttggt ggataaaatt tagaggatga ccctgcacca
     2281 tggacggaat cagctgaccc gtagagtgag gcgtggctcg tcatccgctt ctttcttaac
     2341 tgtgggctgc ttcctcttcc agcgactcga gataacgggt tgcctgaatg gctgccgcag
     2401 cccccatgcc cgccgaggtg accacttgct tgaaggtggc atctgccacc tcccctgctg
     2461 cgaacacgcc gggaatgctg gtttccattt tgtcgttgat cttgagatag ccgagttcgt
     2521 tcatttcgag cttgcctttg aacatatcgg tattcggtgt atgaccaatg aaaatgaaca
     2581 gaccgtctgt atcaaaggtg ctttcttcgt tggtttgaac attcttgagt ttgagggtag
     2641 tgagcttgtc gccttggttc acttcggtga ctacggtatt caagatgaag ttcatcttgg
     2701 ggtgttcttt ggcacggcgt tgcaaaatag caccggcgcg gaattcttca cggcggtgaa
     2761 tgagggtaac ggttgaggca taacgagtga tgaaaagtgc ttcttcaaag gcgctatcgc
     2821 caccgcctac aatgacaacc tttttatctt tgaagaacca gccatcacaa gtggcgcagt
     2881 acgaaacacc gcgaccggtc atttcatatt cgccagggac attaagatga acagggttag
     2941 cacctgtagc gagaatgacg ctatcggcat aataatcgcc actatcggca gtgaccttga
     3001 acgggcgctg cgagaaatcg acttcgtgcg ccatgtcgaa ttcgacttta gcgccgaaat
     3061 gctcggcttg cttttggaag agttcgccaa gctgcggtcc acctacgcct tcggggaagc
     3121 ccgggtaatt ttcgatcgtg tgggttaagg ctgcctgccc gcccaactgc ataccagtaa
     3181 gcaccacagg ggcaagctct gcccgtgcag catagagtgc tgccgaaagc ccggcagggc
     3241 ccgcacccaa gatcaatact tttacatgtc tatt
//
LOCUS       DAOQFT010000270         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17096_length_3271_cov_7.316542, whole genome shotgun sequence.
ACCESSION   DAOQFT010000270 DAOQFT010000000
VERSION     DAOQFT010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17096_length_3271_cov_7.316542"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /gene="lgt"
                     /locus_tag="PLR93_07720"
     CDS             complement(<1..994)
                     /gene="lgt"
                     /locus_tag="PLR93_07720"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH26890.1"
                     /translation="MIDPVIFSFKLFTWQVTLTWYGLIVMSGVLVAAWFAEREVRRRG
                     ENGEVLIDAMVWAVVAGIIGARLWYVMNALIGGNRSYIEDPMSIIRPPIAGLHFFGGL
                     LFGAIALIIYLRNNGYDVWLFMDAVAPVALLGQAVGRIGNYINQELYGPPTQLPWGIS
                     IPADHRLAQYADLAKYPVESTRFHPTFAYEMVLNVLAFLFIMWYARRDEEEPKPGTLF
                     SMWLIAAGFIRTFIEFFRPDQPRLGDTFITTSMLVAFLMGVTGVILFLARHGKLQIAA
                     LTDWEEEYFIKKVEKKSAPRARKVESAAVVETDSEPEVDEPVVKRKSPARAKATT"
     gene            1250..1756
                     /locus_tag="PLR93_07725"
     CDS             1250..1756
                     /locus_tag="PLR93_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNH26891.1"
                     /translation="MFRVNRQTDYAVRVVLALAKKPQGTRLPTAEIGREMLIPPSLLQ
                     RIVAELANGGYIKTQPGRDGGISLAHLPSEITLLQVVEHFEGNLVISDCILKEGDCPF
                     ETKCPVSCQWKRLNDLLIAEMSRITFEQLVEDGKQIEASQMTTTASNQLKVVSNQKSF
                     SLTDSLSA"
     gene            1753..2427
                     /locus_tag="PLR93_07730"
     CDS             1753..2427
                     /locus_tag="PLR93_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH26892.1"
                     /translation="MNKYALNGLYTLFGALAIAAFAFKVFQPIQVLPRMRLSPAYSFI
                     NQDGETVTSEDLRGQFVLYSFTYTNCPAPCYNMGETMKEVQARLNEVDLGGIPVSFVT
                     ISIDPDRDTPEALKTYGTDLGANFDDWMFVTTTNKALLKTIVGSGFETYYEDKGNGSF
                     ALDPGYVLVDGWGIIRAEYRYSTEVSDADRIIRHMGVLADEVKNSKGSTKLAYEAAHL
                     FLCYAP"
     gene            2443..3030
                     /locus_tag="PLR93_07735"
     CDS             2443..3030
                     /locus_tag="PLR93_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH26893.1"
                     /translation="MKIRFFLVGIATILVLIAGIYFLRPHTFHGTVIQSPDPSFDFTL
                     KSADGDVSISDFRGKLVLLYFGYTFCPDICPGTLANVAQALREMGTKADDVQLIMVSL
                     DPERDTPEKLKEYMGHFYPSFVGVTGSKEQLDEITSLYGVFYQKKEGSVATGYLIDHT
                     ATLMVLDREGYLKLVFPFGVTSEEIADDLKYMLRQ"
BASE COUNT          934 a          817 c          708 g          812 t
ORIGIN      
        1 ctgttgtagc cttcgcccga gcaggggatt ttcgttttac aacgggttca tcaacttcgg
       61 gttcgctatc tgtttccaca acagccgcag attctacttt gcgagcgcga ggcgcagact
      121 tcttttccac tttcttgatg aaatactctt cttcccaatc tgtcaatgct gcaatttgaa
      181 gtttgccatg gcgagcgagg aacaaaatca caccagttac gcccatgagg aacgcaacca
      241 acatggaggt ggtaataaaa gtatcgccaa ggcgcggttg atctgggcgg aaaaattcaa
      301 taaacgtacg aatgaatcca gcagcgatca accacatgct aaagagagtg ccaggcttgg
      361 gctcttcctc atcacgacgg gcgtaccaca tgatgaaaag aaaagccaaa acattcaaga
      421 ccatttcata agcaaaggtc ggatgaaagc gcgttgactc taccgggtac ttcgccaaat
      481 ctgcatattg agcaaggcga tgatcagcag ggatcgaaat accccacgga agttgtgttg
      541 gcggaccgta cagttcctga ttaatgtaat taccaattct gcctacagcc tgcccaagca
      601 atgccacggg cgcaaccgcg tccatgaaca gccacacatc atatccgttg ttgcgcagat
      661 aaatgatgag agcaattgca ccaaaaagca aaccaccaaa gaaatgtaaa ccagcgattg
      721 gcgggcgaat aatggacatg gggtcttcaa tataagaacg attgccacca atcagcgcat
      781 tcatgacata ccataagcgc gcaccaataa tgccagctac aaccgcccat accatggcgt
      841 cgatcaatac ttcaccattc tctccacggc ggcgcacttc acgttcggca aaccaagcgg
      901 caaccaacac accagacatc acgatcaagc cataccatgt cagtgtgact tgccacgtga
      961 ataatttaaa actgaaaata acggggtcta tcattcatta cctctgagtt aagttttgac
     1021 gattatatcc ccaaatcaaa ccatacaata catatagccc aatataaggc acattgttac
     1081 tttttgctaa atacaacaaa ttctgtcata aatacttcac gtgctgacaa gaatacgaga
     1141 cgtttgtcat ttaacaatgt gattaataac acgtcaaaag aaagccccaa atccttacaa
     1201 tctcagtaaa cattgacatc aaatgtcaat gttaaaccaa aggatattaa tgttccgagt
     1261 aaaccgccaa accgattatg ccgttcgcgt tgtgctggct cttgcgaaaa aaccgcaagg
     1321 gactcgtttg cctactgcag aaatcggacg cgaaatgctc attccgcctt ctttgcttca
     1381 acggattgtg gctgaattgg caaacggcgg ctacatcaaa actcagcctg ggcgcgacgg
     1441 cggtattagc ttggctcacc tccctagcga aatcaccctg ttacaggtcg tagagcactt
     1501 tgaaggcaat ttggtcatct cagactgcat attaaaggaa ggcgattgcc cttttgagac
     1561 gaaatgcccg gtaagctgcc aatggaagcg cttaaatgac ctactgatcg cagaaatgtc
     1621 tcgtatcacc ttcgaacaat tggtagaaga cggaaaacag atcgaagcca gtcagatgac
     1681 gacaaccgcc tcaaaccaac tcaaagttgt ttctaatcaa aaaagctttt ctctcactga
     1741 cagcctctct gcatgaacaa atacgctctc aacggtctgt acacactctt tggtgctctt
     1801 gcaattgctg ctttcgcatt taaagttttt cagcccattc aagtactccc ccgcatgcgc
     1861 ctctcccctg cttatagttt tatcaaccaa gatggcgaaa cggttaccag cgaagaccta
     1921 cgcggacagt tcgtcctcta ctccttcaca tacaccaact gcccagcacc ttgctacaac
     1981 atgggcgaaa ccatgaaaga agtacaagcc cgcctcaacg aagtggattt gggcggtatt
     2041 ccggtttcat ttgtcaccat ctcaatcgac ccagaccgag atacgcccga agcgttgaaa
     2101 acctacggca cagacctcgg cgcaaacttt gacgactgga tgttcgtcac caccacaaac
     2161 aaggctttac tcaagaccat tgttggctca ggttttgaaa cgtattacga agacaaagga
     2221 aatggtagtt ttgccctcga ccccggttac gttcttgtag atggctgggg catcatccgt
     2281 gctgaatatc gctactccac tgaagtctca gacgctgacc gtatcatccg ccacatgggg
     2341 gttctggcag atgaggtcaa gaacagcaaa ggttctacca aactagcata tgaagcggct
     2401 cacctcttcc tttgttatgc gccataaagg tctatcgtta ttatgaagat ccgatttttt
     2461 cttgttggta ttgcgacaat tcttgttttg atagcaggaa tatatttttt acgtccacat
     2521 acatttcatg gaacggtcat tcagtcccct gacccttcat ttgatttcac ccttaaaagc
     2581 gcagacggcg atgtttccat aagcgacttc cgcggcaaac tggtattgct ttatttcggt
     2641 tacaccttct gcccagatat ttgccccggc acgcttgcaa atgtggctca ggctttgcga
     2701 gaaatgggaa caaaagccga tgatgttcag ctcatcatgg tctcgcttga cccagaacgg
     2761 gatacccctg aaaagttaaa ggaatacatg gggcattttt atccttcgtt tgtgggcgtt
     2821 acaggtagca aagaacaact agacgaaatt acctcactgt atggggtctt ctatcaaaag
     2881 aaggaaggca gtgttgctac cgggtatttg atcgaccata cagctacttt aatggtattg
     2941 gatcgggaag gatatttgaa gttggtattt ccattcggcg tcacttctga agagattgcc
     3001 gatgatctga agtacatgct acggcaataa ggaggtggac ctgctacagc gtagagaact
     3061 gcccattatc caccaccgca gcttgcggcg ccgcgtgaag cggtagtcat cctaatttca
     3121 ttttttcaag aggagaatgt aacatggcga agaaagataa agaagacgat gagtttcgcc
     3181 cgactggcac cattgcagtc ttgacgatat tcgtgatcac actcatcgca atctgggcaa
     3241 cgatctatat gatccttgtc ggacgtggag g
//
LOCUS       DAOQFT010000271         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17100_length_3271_cov_5.896144, whole genome shotgun sequence.
ACCESSION   DAOQFT010000271 DAOQFT010000000
VERSION     DAOQFT010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17100_length_3271_cov_5.896144"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="PLR93_07740"
     CDS             <1..1647
                     /locus_tag="PLR93_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH26894.1"
                     /translation="GNDGIRLDFIDNDSVLDSAGNPTNAGFSNGGSYSIDRTAPVVTS
                     ITPLSQPTETSVDYTVSFSEPVTGVDGSDFFLFTMNGASITNISGAGNQYLVSISLNP
                     GSDSVRLDVNDNDSILDSVGNPLGGIGAFNGNFIGSTFNVAIDTPIATSIIRANNNPT
                     NAANVNFIVTFSEPVDGVDIGDFILSPNAVITNITNSNPFYIVTVNSENINGELQLQL
                     NDDDSIHNTQGIPLGGNGIGNANFATGETYLIDRISPQVTSIIRAGGNPAINPTADFI
                     VTFSEPVNGVETSDFILTSSNVIQSSVVGMQNADPFYWVKVSTGIGSGSIRLGLLDNG
                     NITDKAGNPLLNRNFTGESFNIAKTPVDFSAPTMNSVQGKLTNNPLVSISWSAVRNAQ
                     AYEVFVARDSNFSQIVFIQTTTSTSITPPTPLADGGYYVRVRAYNPDLNPGKFSKAIN
                     FAVDATPPVAPNLLSPASGATTTKRAWLKWSVVNSVVKYQIQVDNNSDFSSPEFDATT
                     SKATIQTKSLPARTYYWRVRGIDSAGNWSAWSQTFLFTTK"
     gene            1719..2342
                     /locus_tag="PLR93_07745"
     CDS             1719..2342
                     /locus_tag="PLR93_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNH26895.1"
                     /translation="MKSTRDKILQTLLKKPKSTINDLADAVGINPISVRHHLTNLQME
                     GLVESQEERHGVGRPRLVYVLTDEGMERFPTRYLRLTTRLIAQMKESIPGPMVSKLFG
                     QIAEDLANEYANDIKGLSMEERLDFVKDMLAQEGFTVEWEKKDGQYQIHEISCPYYQI
                     GVAHPEVCTVDQTLISKMLALPANKVRCILDGGAHCTYVVQPVGKTK"
     gene            2355..2702
                     /locus_tag="PLR93_07750"
     CDS             2355..2702
                     /locus_tag="PLR93_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNH26896.1"
                     /translation="MSDSQINEIQWTIHTTNPDLVKQARASLSEVVDPEIGMNIIQLG
                     LVRDIEVENGMARLKMILTTPFCPYGPAMIEMTKAKATEGLNMPVTVEMGMDVWDFSM
                     MEDPSALDWGMYA"
     gene            complement(2765..>3271)
                     /locus_tag="PLR93_07755"
     CDS             complement(2765..>3271)
                     /locus_tag="PLR93_07755"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH26897.1"
                     /translation="RSGLSANALSMIERGKTSPSVSTLYKLANALGVSITAFFDTETE
                     KRQVVFLKSDGRTRLSFTRGVFEALGGESFVGRVEPFMLTMESGASCGPHDIVHSGHE
                     FVFCLRGQLDYTVEKETFRLEPGDSLLFASKLRHRWKNPANVVANALIIISGFAEGEE
                     PHSMHWNK"
BASE COUNT          943 a          826 c          727 g          775 t
ORIGIN      
        1 gggaatgacg gaatacgtct ggattttatt gataacgata gcgtattaga ctccgcaggt
       61 aacccaacta atgctgggtt ctcaaatgga gggtcttata gcattgaccg aaccgcacct
      121 gtggtcactt ctatcacacc attaagtcag ccaaccgaaa ccagcgtaga ctacactgtc
      181 agtttttctg aaccagtcac cggcgtggat ggaagcgatt tcttcctctt cactatgaac
      241 ggagcaagca tcacgaatat ctctggtgcc gggaatcaat atcttgtttc catctcgctc
      301 aaccccggaa gcgacagcgt ccgcttagat gtaaatgaca acgatagcat tctggatagt
      361 gtcggtaacc cgctaggagg gatcggggcg ttcaatggaa acttcattgg cagcacgttc
      421 aatgttgcaa ttgacacgcc gattgcaaca tcgatcattc gcgccaacaa caacccaaca
      481 aatgccgcaa acgtgaactt tatcgtcact ttctctgagc cggttgacgg tgtggatata
      541 ggcgatttta ttttgtcacc caatgctgtt attaccaata tcacgaactc gaatccgttt
      601 tacattgtta cggttaactc agaaaatatt aatggcgagc tacagcttca attgaacgat
      661 gacgatagca tccataatac ccaaggcatc ccactcggcg ggaacgggat aggaaatgca
      721 aattttgcaa caggtgaaac ctacctcatt gaccgtatat cgccgcaagt gacatctatt
      781 attcgcgcag gtggtaatcc cgccatcaac ccaacggcag atttcatcgt cacattttcg
      841 gagcccgtga acggcgtgga aacaagtgac tttattttga ccagttccaa tgtcattcaa
      901 tcgagtgtgg tgggcatgca aaacgcagac ccattttatt gggtcaaggt cagtacaggg
      961 atcggctcgg gaagcattcg gctcggccta ttggataatg gaaacattac agacaaagcc
     1021 ggaaacccac tcctgaatag gaatttcaca ggcgagagtt tcaatattgc caaaacccct
     1081 gtggattttt cggcacccac catgaattcc gttcaaggca aattgaccaa caacccatta
     1141 gtctctatat catggtctgc ggtgcgtaat gcacaggcat atgaagtatt cgttgcacgc
     1201 gatagtaatt tttcacaaat tgtttttatt caaacaacaa cctctacatc cataactccg
     1261 cctactccac ttgcagatgg cggctattat gtacgagtcc gcgcttacaa cccagaccta
     1321 aaccccggga aattcagcaa agcaatcaac tttgcagtag atgcaactcc gcccgttgcc
     1381 cccaaccttc ttagcccagc ctctggtgca accacaacca aacgggcatg gttgaaatgg
     1441 tctgtagtaa acagcgttgt gaaatatcaa attcaagtgg acaacaactc cgatttttca
     1501 agccctgaat tcgacgcaac gaccagcaag gcaacaatac aaacaaaaag cctgccagct
     1561 agaacttact attggagagt tcggggcata gacagtgctg ggaattggag cgcctggtct
     1621 caaaccttcc tattcaccac aaaatagccc tctcgtaatt tttatgatat ttatctagaa
     1681 tattgacaag agcctacagg gaagttatac tgccctgcat gaaatctacg cgagacaaga
     1741 ttttacaaac tttactgaaa aagccaaaat ccacgatcaa tgacctggca gatgccgtcg
     1801 gcatcaaccc gatctctgtg cggcatcacc tcaccaacct gcaaatggaa gggctggttg
     1861 aatcgcaaga ggaacgccac ggggtaggac gcccacgttt ggtctatgta ctgaccgatg
     1921 aaggcatgga acgattccct acccgctacc tgcgtctgac aacccgcctc attgctcaga
     1981 tgaaagaaag catccccgga cccatggtga gcaagttgtt cggtcagatc gctgaagacc
     2041 tagccaatga atatgccaac gatatcaaag gtctaagtat ggaagaacgc cttgacttcg
     2101 tcaaggatat gttggcgcag gaaggtttta ctgtggaatg ggaaaagaaa gatggacaat
     2161 accagatcca cgaaatctcc tgcccatact atcagatcgg cgtggcacac ccagaagtct
     2221 gcacggtaga tcaaactctg atctccaaaa tgctggcact gcctgcaaac aaagttcgct
     2281 gcattttaga tggcggtgct cattgtacgt atgtggttca acccgtcggt aaaacaaaat
     2341 aaaaagagga ctttatgagc gattctcaaa ttaacgaaat ccagtggacc atccacacca
     2401 ccaacccaga cctggtcaag caggcgcgcg ctagtttgtc tgaagtagtg gaccctgaaa
     2461 ttggtatgaa catcatccaa cttggcttgg tacgtgatat cgaagtggaa aacggtatgg
     2521 cacgcctcaa aatgatcctg accacgccct tctgcccgta tggtcctgct atgatcgaaa
     2581 tgaccaaagc caaggccact gaaggcttga acatgcccgt cactgtagaa atgggcatgg
     2641 atgtgtggga cttctccatg atggaagacc cctctgcact tgactggggt atgtacgcgt
     2701 agttttagta aaagagcaaa aagagccacc tgtacaggtg gctcttttta tttaacatct
     2761 taatttattt attccaatgc atagagtgag gctcttcgcc ctcagcaaag ccagagataa
     2821 tgatcagtgc gttcgcaacg acattagcag gattcttcca gcgatggcga agctttgaag
     2881 cgaagagcaa gctgtccccc ggttcgaggc ggaaggtctc tttttccacc gtgtaatcta
     2941 attgaccacg cagacaaaaa acgaattcat gccccgaatg gacaatatca tgcggaccgc
     3001 aggaagcgcc actttccatc gtcagcataa aaggctccac ccgcccaaca aagctttcgc
     3061 cgccgagagc ttcaaacacg ccccgagtga aagataaacg ggtgcgtcca tcgctcttca
     3121 gaaaaacaac ttgtctcttt tcggtctcgg tatcgaaaaa cgcagtaatt gaaacaccca
     3181 atgcattcgc taacttgtac aaagtactca cagatggtga ggtcttcccc cgctcgatca
     3241 tagacaatgc atttgcagaa agcccgcttc g
//
LOCUS       DAOQFT010000272         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17144_length_3266_cov_4.199626, whole genome shotgun sequence.
ACCESSION   DAOQFT010000272 DAOQFT010000000
VERSION     DAOQFT010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17144_length_3266_cov_4.199626"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLR93_07760"
     CDS             complement(<1..910)
                     /locus_tag="PLR93_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNH26898.1"
                     /translation="MSFANLLTQEQVERIHEVSLEILEEVGLKVRFEPAREMFKAHGC
                     HVEEERVRFPRAVVEKYRKMVPASFTFHGRDPKFDKTIPQDSPVIVTASSAPDIIDPV
                     TGKERRAESKDIAQIAHLINELPGYDIFSISTLADDAPAGQFTISRLYPALKYCLKPL
                     RVTTTDMKDTMSVMRMAYMVAGGEEAYKAQPFLTHHYCPVVSPLTMDHLSTENVMFFA
                     SQGLPVYPTIVPNAGLTSPMSMAGTLAQGNAEFLSALMLMQMTKEGTPTIYATLGTVA
                     DMRSGAYTSGGIECGMLHMAFAQMARF"
     gene            complement(943..2037)
                     /locus_tag="PLR93_07765"
     CDS             complement(943..2037)
                     /locus_tag="PLR93_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH26899.1"
                     /translation="MAKKKLSPPAYVGFGMLTPVYIMSLDQLPKLNTGAIVHQVSDYV
                     YDDAAIIACNLSQWGVSSGMIGTSVGDDMLGRYVAEKLKTMGVQGKVRVTDQYKTPLE
                     VNVSDKKGARTYFWQRSKEILNTLETADLSLIKNAQLLYVDWYDGNHIVRAMEEAKRH
                     HVPVFLNFEHGHQNSELLKKYASMVTICQAVTDAAQIGKKQAMLGTARKLIKAGIQTA
                     IITMASQGCLVVQGDEIIRAYAPKVKAVDASGAGATFSSGFVYGYLNDFNLEDTIRFA
                     IAAASLKVTRSGLEMFPVQEIQGLARTVRIEHMVYRGDQFRTIRKFLSLPKDNPLMNN
                     VLVKNSQILAERILPKKKLDRKRIKRSLVE"
     gene            complement(2085..>3266)
                     /locus_tag="PLR93_07770"
     CDS             complement(2085..>3266)
                     /locus_tag="PLR93_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HNH26900.1"
                     /translation="PVHYRLMENGAVFGEKFGWERVNYFDPGKDWRRMGEDQRLWGWA
                     KPPFFERLRVEHTAARERVALFDLTSFGKIEVKGKGALPLLQRLTSSNIDKPVGSAIY
                     TQFLNKNGGIESDLTVTRLGEEYFWVITGSGFIANDHARILMNVDEKDGEVSIRDITQ
                     EHACLALWGPKAREVLKKITSSDVSNQAHPYLTTKPITINGVKVLAQRVSYAGELGWE
                     LYIPNNRATMVWDMLIEAGKEFGMELGGYKVLDPLRLEKGFKYFTTDITPTTTPYEAG
                     LGFCVDLEKGDFIGRDALVKQKAEGLTRKLCTMVLTGTDEFVQIYGGEAVYYGDKLVT
                     RVRSGGYGFTVKKNILYAYLPIDLAVKGNRFTIELIEGNLEAEVTANVLYDPKSEKLK
                     A"
BASE COUNT          835 a          919 c          695 g          817 t
ORIGIN      
        1 agaatcgcgc catctgtgca aaggccatat gcagcattcc acattcgatc ccgccggagg
       61 tataggcccc ggaacgcata tcggcaaccg taccaagcgt ggcatagatg gtcggcgtgc
      121 cttcctttgt catctgcatc agcatcaaag cagataaaaa ttcggcattg ccttgcgcca
      181 aagttcctgc catagacatg ggcgaggtca aacctgcgtt cggcacgatg gtcgggtaaa
      241 ctggcaaacc ctgactggca aagaacatta cattttccgt ggagagatga tccatcgtca
      301 atggcgagac aacaggacaa taatgatgtg tcaggaacgg ttgagccttg tacgcttcct
      361 ccccacccgc caccatgtat gccattcgca tcacgctcat ggtatctttc atatctgtgg
      421 tggtaacacg caacggtttg agacaatatt tgagagctgg atacaggcgc gagattgtga
      481 attgacccgc aggcgcgtca tctgccagag tggaaataga gaagatgtca taccccggca
      541 gttcattgat gagatgcgcg atctgcgcaa tatctttgga ctcagcgcgt ctttccttgc
      601 cggtcactgg gtcaataata tcgggcgctg aactagccgt aacaatgaca gggctatcct
      661 gggggatcgt tttatcaaac ttcgggtcgc gtccgtggaa tgtgaaagac gcgggaacca
      721 tctttcggta tttttcaacc actgcacgcg ggaacctgac acgttcctcc tccacatgac
      781 aaccatgtgc cttaaacatc tcacgcgcag gttcaaaccg aaccttcagg ccaacttcct
      841 ccaaaatttc cagtgaaact tcatggatcc gttcaacctg ttcctgggtt agcaaattcg
      901 caaatgacat gatggggttc ctttataaat aaaatggatc gatcattcga caagcgatct
      961 cttaattctc tttctatcca actttttctt tggcaatatt ctctccgcaa ggatctggct
     1021 gttctttaca agaacattgt tcatcaaggg attatccttc ggcaaactca agaatttgcg
     1081 gatcgtgcgg aactggtcac cccgataaac catatgttca atacgcaccg tgcgcgccag
     1141 tccttgaatt tcctgcacag ggaacatttc caaaccagaa cgggttacct ttagcgaagc
     1201 cgccgcaatt gcgaaacgaa tcgtatcctc caaattgaaa tcgttcaagt agccatacac
     1261 aaatcccgaa gaaaatgttg cacccgctcc cgaggcatcc acagccttca cttttggagc
     1321 atacgcccgg atgatctcat ccccttggac gaccagacag ccctgactcg ccatcgtgat
     1381 gatcgccgtt tggatacccg ctttgatcaa tttacgcgcc gtccccaaca tggcctgctt
     1441 tttaccgatc tgggccgcat ccgtcacggc ctgacaaatg gtgaccatgc tcgcatattt
     1501 tttcagcagt tccgagtttt gatgcccatg ctcaaaattc aaaaagacag gcacatgatg
     1561 tcgcttcgct tcctccattg cacggacgat atgattgccg tcataccaat ccacataaag
     1621 caattgggca ttcttaatca gggaaagatc tgctgtttca agagtattaa gaatttcctt
     1681 ggaccgctgc caaaaatatg tacgcgcacc tttcttatcc gatacattaa cctcaagggg
     1741 cgtcttgtat tgatcggtaa ctcgtacctt cccctgaacc cccattgtct tcagtttttc
     1801 cgccacatac cgcccaagca tatcgtctcc aaccgatgta ccaatcatgc cagaggagac
     1861 tccccattga cttaggttgc atgcaatgat ggccgcatca tcatacacat aatcgctgac
     1921 ttgatgcaca atggcgcccg tattcaactt cggcagttga tccagtgaca tgatatacac
     1981 cggcgtcaac atgccaaaac caacgtaagc aggaggagaa agttttttct ttgccataaa
     2041 tcattcccgt cattgcgagg agcattctct tcgcaatgac gaaactacgc cttcagtttc
     2101 tcgcttttcg gatcgtacag cacattcgca gtcacttccg cttcgaggtt gccttcaatc
     2161 agttcaatag tgaagcgatt acccttcacc gccaggtcga tcggcagata agcatacaga
     2221 atgttctttt tcacagtaaa cccgtaaccg ccgctgcgaa cacgagtcac aagcttatct
     2281 ccataataca ccgcctcgcc gccatagatt tgaacaaatt catccgtgcc tgtcaacacc
     2341 attgtacaaa gcttgcgcgt caacccttcc gctttttgct tcaccaaggc atcgcgccca
     2401 atgaagtcgc ctttttccaa gtccacgcag aaaccaagac cagcttcata cggggtggtt
     2461 gttggcgtaa tatcagtggt gaaatattta aaaccttttt ccaaacgcag cggatcaagc
     2521 accttgtacc cgccaagttc catgccaaat tctttgcccg cttcgatcag catatcccac
     2581 accatggtgg cacggttgtt gggaatgtac aactcccaac ccaactctcc ggcataactg
     2641 acacgctgcg ctagtacctt cacaccatta atggtgatgg gcttggtggt caaatacgga
     2701 tgtgcttgat tggaaacatc gctgctggta atcttcttca aaacttcgcg ggctttcggc
     2761 ccccacaatg caagacaggc atgttcctgc gtaatatcac ggatggacac ttcgccgtct
     2821 ttttcatcca cattcatcaa aatacgggca tggtcattgg caatgaagcc cgaccccgtg
     2881 atcacccaga aatactcttc acccaagcgt gtcaccgtca agtctgattc aatgccgcca
     2941 tttttgttga ggaattgagt atagatggcg ctgcccacag gtttatcaat attgctcgaa
     3001 gtcagtcgct gcaacaaagg caatgcgcct ttgcctttga cttcaatttt gccaaacgaa
     3061 gtcaaatcaa acagcgccac gcgttcacgg gcagcagtat gctccacgcg cagacgctca
     3121 aagaacggag gctttgccca cccccacaga cgctgatctt cgcccatgcg ccgccaatct
     3181 ttgccggggt caaagtagtt cacacgctcc cagccaaact tctcgccaaa gaccgcaccg
     3241 ttttccatca aacgataatg caccgg
//
LOCUS       DAOQFT010000273         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17190_length_3258_cov_6.892913, whole genome shotgun sequence.
ACCESSION   DAOQFT010000273 DAOQFT010000000
VERSION     DAOQFT010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17190_length_3258_cov_6.892913"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLR93_07775"
     CDS             complement(<1..676)
                     /locus_tag="PLR93_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26901.1"
                     /translation="MIRNFTFVFLLFVLMIGITGCNLPLPTCATDSLQEVTLLSPAKM
                     GVVDTLEPTLEWSYPSSECTPEGYAITMWEGPVMTENIGGGTGNPNTQWVPGSPLEPG
                     KQYRWTVAPINGTTLGPVSETFVFFTGPVCTDASLVPPTMLEPADGASFNATTDLLWW
                     DYTAGCTPTGYQLDVATDSSFASGLYPSGIIDIPLTRAVLGDPPLVECQTYYWRVAAR
                     NGDTIGS"
     gene            833..1591
                     /gene="trmD"
                     /locus_tag="PLR93_07780"
     CDS             833..1591
                     /gene="trmD"
                     /locus_tag="PLR93_07780"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNH26902.1"
                     /translation="MQFEVFTLLPEVFPSYLDTSILKRARERGLIHVNVHNIRDYTHD
                     RHNTTDDQPYGGGGGMVMKPEPVFEAIETVLGLASPPSTPEPEANIPIILLTPQGRVF
                     NQSIAKELSQHKKIALLCGRYEGIDERIRENLVTDQISIGDYVLTGGELPALILIDAV
                     SRLLPNVLGDPTGAEDDSHAMGLLEYPHYTRPPEFRGSKVPDVLLSGDHKKIDKWRRE
                     QALLRTLQKRPDMLETAELTKEDKKFLEKLKNQS"
     gene            1651..2934
                     /locus_tag="PLR93_07785"
     CDS             1651..2934
                     /locus_tag="PLR93_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26903.1"
                     /translation="MSSSRLNYGKTFLLGFGFFGVSVIWGVYNAFVPIFLSEKFNLSP
                     VLIGFFMTLDNIAALFIQPPVGAWSDKLRTPIGRRIPFILIGAPITALAFGLIPIAAV
                     LPLFVACTSTLLLSAALWRTPVVALMPDITPSEFRSQANGIINFMGGIGTIIALQTGG
                     ILYKMSPSFPFWLGSGLVLIAALIVYLFIEEPKEYEAAEEQPGMFASLKEVVSDPDKS
                     GLRILLAIFFWFIGFSAVETFFTLYAKNHLGLNEGDGATLLSVLPLFFVLAAIPSGYV
                     AGKLGRKTTIATGLIIITIMLVLLYITPAEALLTGIAPLPLVGIPLVEGGARMLTLAG
                     VLLIIGGIGWAFININSLPMIVDLTSAARIGTFTGLYYLFSTLSAIVGPNLNGWAVQL
                     TNGNYNIIMLIAPFFMLIALGLMMGVKKGEAITSN"
     gene            2957..>3258
                     /locus_tag="PLR93_07790"
     CDS             2957..>3258
                     /locus_tag="PLR93_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26904.1"
                     /translation="MFQTRNHLSRITKYFYLAMMAWALSACTSSPDCFRKDVFCAALV
                     TDTLGIEDHGMNQNAWAGLQATQENGLADQVDYIASVDARDYQKNITYFVDLGYDV"
BASE COUNT          821 a          861 c          722 g          854 t
ORIGIN      
        1 acgagccaat agtatcgccg tttcttgcgg caacacgcca gtaataggtt tgacactcca
       61 ccaatggagg atcgcccaac acagctcttg ttagtggaat atcaataatc ccagatggat
      121 atagtccgct tgcaaaagat gaatccgttg cgacatccag ttgataccct gtaggcgtac
      181 accctgccgt gtaatcccac cataataggt ctgtcgttgc attgaaactt gctccatcgg
      241 ctggctcaag catagtgggg ggaacaagag acgcatcggt acacactggt cctgtaaaga
      301 aaacaaacgt ttctgaaacc ggtcctagtg tcgtcccatt gatgggcgca actgtccagc
      361 gatattgttt accgggctca aggggtgaac ctggtaccca ctgggtgttg gggttacccg
      421 ttccaccacc aatgttctca gtcatcacgg gtccttccca catcgtaatg gcataccctt
      481 ctggcgtaca ctcagaagat ggataactcc actcaagcgt gggttctaac gtatcaacaa
      541 cacccatctt tgcagggcta agcagagtga cttcttgtaa actatctgta gcgcaggtag
      601 gcagcggtaa attacacccc gttattccga tcatcaaaac aaaaagcagg aacacaaaag
      661 taaaatttcg tatcatctca agttctcctc agtaaaatga atgacattgg acgcaaatgc
      721 gccaaagtgg aaccttgaat aaattgcctg cttatctaat aaagaaattt tatcgtttat
      781 tgccttagag cgtagcttac cgaagtacta tttcctatgc tacaatcgcg ccatgcaatt
      841 cgaggttttt acactcctcc ctgaagtttt cccctcttat ttagatacta gcatcctcaa
      901 acgcgcccgt gaacgcggat tgatccatgt caacgttcac aacattcgtg actacaccca
      961 cgacagacac aacaccaccg acgatcaacc ctacggcggc ggcggcggaa tggtgatgaa
     1021 gccagagccg gttttcgaag ccattgaaac cgttctggga ctcgcttcgc ctccttccac
     1081 gccggaacct gaagcaaaca tcccaatcat tttgcttacg ccgcaaggac gcgtcttcaa
     1141 ccaatccatt gccaaagaat tatcacaaca caaaaagatc gccctgctat gcggacgtta
     1201 tgaaggcatt gacgaacgca tccgcgagaa cttggtgacc gaccaaatct ccattggcga
     1261 ctatgtgctc actggtggcg aactgcccgc gctcatccta attgatgcgg tttctcgtct
     1321 cctgcccaat gtacttggcg accccaccgg cgccgaagat gactcgcacg ccatgggctt
     1381 gttggagtat ccccattaca cccgtccgcc tgagtttcgc ggtagcaaag tgcccgatgt
     1441 gctcctatct ggtgaccaca aaaaaattga caaatggcgc agagaacaag cactcttacg
     1501 cacacttcaa aagcgccccg atatgcttga aactgcagag ctcacaaaag aagataaaaa
     1561 gtttcttgag aagctaaaga atcaaagtta aaacgttggc agacttaagc atttaaacct
     1621 accaacctgt aaaccaacct ggaggctatg atgtcatcgt caagattgaa ctacggcaag
     1681 accttcctgc tcggcttcgg tttttttggt gtgagtgtga tctggggtgt gtataacgcc
     1741 ttcgttccca tttttctctc tgaaaaattc aacctctccc ctgtgttaat cggtttcttc
     1801 atgacccttg ataacattgc ggcattgttc attcagccgc ctgttggagc atggtctgat
     1861 aaattgcgca cccccattgg tcgccgcatt ccatttattt tgatcggcgc acccatcacc
     1921 gccttagcat tcggcttgat tcctattgca gcagtgcttc cgctatttgt ggcatgtacc
     1981 agcacccttt tgctcagtgc tgctttatgg cgcacccctg tagtagcgct aatgcctgat
     2041 atcacaccct ctgaatttcg ctcgcaagcc aatggcatta tcaacttcat gggcggaatt
     2101 ggcaccatca tcgccctgca aaccggcggc atactttata aaatgagtcc cagcttccca
     2161 ttctggttag gctctgggct cgtgcttata gctgcgttga tcgtatatct tttcattgaa
     2221 gagcccaaag aatacgaagc cgccgaagaa caacccggta tgtttgccag tctcaaagaa
     2281 gtagtcagcg accccgataa aagcggtttg cgcattttgc ttgccatctt tttctggttc
     2341 attggttttt cagcagtaga aactttcttc acactttatg ccaagaatca tctcggcttg
     2401 aacgaaggtg acggcgcaac cttactctcc gtcctgccac tgttctttgt gctggctgcc
     2461 atcccttctg ggtatgtggc tgggaagctt gggcgcaaaa ccaccattgc cactggtttg
     2521 atcattatca ccatcatgtt ggtcttgcta tatatcaccc ctgctgaagc gttgctcacc
     2581 ggcattgcgc ctctgccact ggtcggcatt ccgctcgtgg aaggcggcgc acgcatgctc
     2641 acactggctg gcgttttgct catcatcggc ggcatcggtt gggcattcat caacatcaac
     2701 tctctgccca tgatcgtgga tttgaccagt gcggcgcgca tcggtacatt cacaggtctc
     2761 tattatctct tctccacctt atctgccatt gtgggtccca acctgaatgg ttgggcagta
     2821 caactcacga atggcaatta caacatcatc atgttgatcg ctccattctt tatgttgata
     2881 gcactcggtt tgatgatggg cgtcaaaaaa ggtgaggcaa tcacaagcaa ttaatgagta
     2941 ccaagtgaag agcaatttgt ttcaaacaag aaatcacctt tcacgaatca cgaagtattt
     3001 ttatctggcg atgatggcat gggcactgag tgcctgtacg tcatcgccag attgttttcg
     3061 caaagatgtc ttctgcgcgg cattggtaac tgacactttg ggtattgaag accacggcat
     3121 gaatcaaaat gcatgggcag ggttacaagc aacgcaagaa aatggcttgg cagatcaagt
     3181 ggattacatc gcctctgtcg atgcgcgcga ttatcaaaag aacatcacgt attttgtaga
     3241 ccttggctac gacgtcat
//
LOCUS       DAOQFT010000274         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17208_length_3255_cov_5.986562, whole genome shotgun sequence.
ACCESSION   DAOQFT010000274 DAOQFT010000000
VERSION     DAOQFT010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17208_length_3255_cov_5.986562"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="PLR93_07795"
     CDS             complement(<1..1432)
                     /locus_tag="PLR93_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH26905.1"
                     /translation="MSIRLRITFLYTFILALTLTVFGLALYTIQAQSTMDSLKHDLSV
                     SANRLADATLKTDFPNFNGNPPPNGQEPPNGQPPNGQEPPPGGQQPGDGNPPPTPIPF
                     DQFSNQQEFQSFSEREIARVLDVQGNLVSSPFGREGDALPLSAAGLQALQNGSDWWET
                     NIVSDESMLTYNRPVIVDNKVIYIVQVARSLTERNRTLQSLSTTLLLAGLLTTLIAFG
                     IGWLFSGLTLAPINRITKTAKEIGEERDFSRRVDYNGPQDEVGQLASTFNAMLTRLQD
                     AFKKVEHSLQMQRNFVADVSHELRTPLTTLRGNLGLLKRNPSPEIQNDILSDMVDESD
                     RLIRLVNDLLLLARADAGRSLSKEPVDVSIVLQEVCRQGNQLDANREINLDVPADTSI
                     IGDRDAFKQVALILLDNALKHSTGKINIRAEKYGKHIDVRVQDFGEGIPPETLAHVFD
                     RFYRGEDAATIPGFGLGLPIAKTLVEG"
     gene            complement(1432..2112)
                     /locus_tag="PLR93_07800"
     CDS             complement(1432..2112)
                     /locus_tag="PLR93_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH26906.1"
                     /translation="MPSVLVIDDDPKLLKMLQRTLTYENLEVFIANNGLEALPLVQAQ
                     KPDLIIVDWMMPKMDGISFIQRLRDEENQTMILMLTAKDAIDDRVDGLESGADDYLIK
                     PFAPAELVARVHAMLRRVETKPENQKVSYADVSLDPTSREAKRGTIELNLTVTEFNLL
                     HMLLRHPRQVLERRQILNEVWGYDFGGDDNVLEIYIGYLRKKLEVDGNSRLIQTVRGV
                     GYVLREES"
     gene            2267..>3255
                     /locus_tag="PLR93_07805"
     CDS             2267..>3255
                     /locus_tag="PLR93_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26907.1"
                     /translation="MIRKITFIHLAVICALLLTACGTSAASTQETVSATDTSSVVFIT
                     QTAEPTTQNADALPAGGVPNGNPPPGNPPGGGNMGGNLEDGLATATGIYTLSGSVAEE
                     TNQTYTATQQDQSGVYVTDGGVLTLNNVTVNTSGETSSSDGSSFYGLNAGILAVESTI
                     TVNGGTITTSGSGANGAFATGEGSVVNLSNVTITATGDGGHGVMATLGGEVNLTNVNM
                     TTSGAHSAPIATDRGSGTVNSTGGSLLTTGADSPCYYSTGVLNIENSTCTADGSEAAV
                     IEGANSINLIDSDVTVNVPDKWGVMIYQSFSGDAEGTEGIFTMQGGSLTYTDA"
BASE COUNT          821 a          711 c          818 g          905 t
ORIGIN      
        1 acccttcaac aagagttttt gcgattggca gacccagccc aaaccccgga atggttgcgg
       61 catcttcccc gcgatagaaa cggtcgaaga catgcgcaag ggtctcgggt ggaatccctt
      121 cgccgaagtc ttgaacgcga acgtcaatgt gctttccata tttttcagcg cgaatgttga
      181 tcttgcctgt ggagtgctta agtgcattat ctagcaggat cagtgcgacc tgtttgaagg
      241 catcacgatc tccaataatc gaagtgtcag cggggacatc taggttaatc tctctatttg
      301 cgtcaagctg atttccctgc ctgcatactt cttgaagaac aatagaaaca tccactggtt
      361 ctttcgagag actacgcccg gcatcggcgc gtgcaagcaa aagcaggtcg ttgacgaggc
      421 gaataagacg atcgctttca tcgaccatat cgctaagaat atcattttgg atctctggcg
      481 aaggattgcg tttgagcagc ccaagatttc cgcgcaatgt ggtaagcggt gtgcgcaatt
      541 cgtgtgagac atcggcaaca aagtttcgct gcatttgcag agagtgctct acttttttaa
      601 atgcatcttg caagcgggta agcatggcat tgaaggtact cgcgagttga ccaacttcat
      661 cttgtggacc gttgtaatcg acccggcgag aaaaatctcg ctcttcaccg atctctttgg
      721 cagttttggt gatgcggttg atgggggcta atgtaagacc tgaaaaaagc cagccaattc
      781 caaaagcaat cagtgttgta agtaaacctg caagcaataa tgttgtagat aaagattgca
      841 gtgtgcgatt tcgttcggtg agtgaacgag ctacctgaac gatataaata actttattat
      901 ctacaatgac ggggcggttg taggtaagca tgctttcatc tgaaacaatg tttgtttccc
      961 accagtcgct gccgttttgt aaggcttgca acccagcggc gcttaaaggc aatgcatccc
     1021 cttcgcgtcc aaaggggctg ctgaccagat taccttgaac atctaggact ctggcgattt
     1081 cacgttctga gaatgattga aattcttgtt gattggaaaa ttgatcgaac ggtatggggg
     1141 taggaggtgg gtttccatct ccgggttgtt gcccccctgg tggaggttct tgtccgttgg
     1201 gtggttgtcc atttggaggt tcttgcccgt tcggaggagg gttgccattg aaattgggga
     1261 aatccgtttt cagtgttgca tctgctaaac ggttggcgct cacgcttagg tcatgtttga
     1321 gcgaatccat tgtcgattgt gcttgaattg tgtatagcgc cagaccaaag acggttaacg
     1381 tcaacgcgag gatgaatgta taaagaaaag tgatgcgaag ccgaatggac attatgattc
     1441 ttcacgcaga acgtagccaa ccccgcggac ggtttgtatt aggcgcgaat tcccgtctac
     1501 ttcaagtttt ttacgcagat atccgatata gatctcaaga acgttatcat ctccgccaaa
     1561 gtcgtagccc cagacctcat tgagaatctg acgccgttca agcacctggc gtggatgccg
     1621 caatagcata tgcaaaagat tgaattccgt cactgtgaga ttgagttcga tggtaccacg
     1681 tttcgcctcg cgcgatgtgg ggtcgaggct gacatctgca taagagactt tctgattttc
     1741 tggttttgtt tctactctgc gcagcatggc atgaaccctc gcaaccagtt cagctggggc
     1801 aaaaggttta attagatagt catcagcgcc gctttccaac ccgtctacgc ggtcgtcaat
     1861 ggcatctttc gctgtgagca tcagaatcat ggtttgattt tcttcatctc gtaaccgttg
     1921 aataaaagag atgccgtcca ttttgggcat catccaatct acaatgatca ggtctggttt
     1981 ttgcgcttga acaaggggaa gggcttccag tccattattg gctatgaaaa cttcgaggtt
     2041 ttcataggtc aatgttcgct gaagcatctt tagtagtttg gggtcatcat caataacaag
     2101 gacagagggc ataggatctc gcttttttat cttactgcac caactataaa tgaaattctt
     2161 aagctgagaa tgtactcaca gcatattcac agtttcttct ttgcatttcc ataggttcac
     2221 attctagaat gcatatgctt gaaaatatat tctttggaga accaatatga tacgcaaaat
     2281 aacgtttatt catctggctg tcatttgtgc cttgttatta accgcttgcg gcacaagtgc
     2341 ggcatctact caggaaactg tttcggctac agatacatcc tcagttgttt ttatcactca
     2401 aactgctgag cctacaaccc aaaacgcaga tgcgcttcct gctgggggag taccgaatgg
     2461 caatccgcct cccggaaacc cgcccggtgg cggtaacatg ggtggaaact tagaagacgg
     2521 cttggctacg gctacaggta tttacacatt gagcggcagt gtggctgaag aaaccaatca
     2581 aacatataca gccacacagc aggatcaatc tggagtgtat gtcaccgatg gtggtgtgtt
     2641 gactttgaac aatgtcacag ttaatactag tggcgaaaca tcttcgtctg acggcagtag
     2701 tttttacggt ttaaatgcag gcattttggc agttgagtca acgattacgg tcaacggtgg
     2761 gacgatcact accagcggct ctggcgcaaa cggtgctttt gcaacaggcg aaggctcggt
     2821 ggtgaatttg agtaatgtta ccattacggc aaccggagat ggcggacatg gtgtgatggc
     2881 aactcttggc ggagaggtaa atctaactaa cgtgaatatg accacttctg gtgcccattc
     2941 tgctcctatt gctaccgatc gcggcagtgg tacagtcaac tctactggcg gttctttact
     3001 tacgaccgga gcagattcac cttgttacta ttctacaggt gtcttgaata ttgaaaatag
     3061 cacgtgcacg gcagatggtt ccgaagctgc tgtgattgaa ggtgctaatt ccattaatct
     3121 aattgatagt gatgtgacgg ttaatgtccc agataaatgg ggtgtgatga tttatcaaag
     3181 cttttctggc gatgcagaag ggaccgaagg catttttacc atgcaaggcg ggtcgttgac
     3241 gtatacagat gctaa
//
LOCUS       DAOQFT010000275         3229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17430_length_3229_cov_6.915879, whole genome shotgun sequence.
ACCESSION   DAOQFT010000275 DAOQFT010000000
VERSION     DAOQFT010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17430_length_3229_cov_6.915879"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1188)
                     /locus_tag="PLR93_07810"
     CDS             complement(169..1188)
                     /locus_tag="PLR93_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH26908.1"
                     /translation="MKNNLSSITIKDVAKHAGVSIATVSRVLNGSVPVQADKAERVRT
                     AMDDLRFVPRTAARTLASRKTNTIGLILSEIGGAFFPLLLKGIESETRAMGYELLIHS
                     TQGAHASQRKTLGEHNTDGLLVFTSSLAAEELERLHKIKFPLVLMHQTPPDGLDMPVV
                     TIENKDGAEMLVSHLIEKHGRRRIVYMRGPEGHEDSLWRERGYREALEKHNLPFAPEL
                     VVMGGFEEEIAFNNIQKMIQEGIQFDAVFAGDDDTAIGVYRALKASKRLIPDDVAVVG
                     FDDVQFSRYISPALTTVRAPIEEVGREAVRQLKSLMDDGQAESLVLMRTELVIRESCG
                     CKNHP"
     gene            1569..2615
                     /locus_tag="PLR93_07815"
     CDS             1569..2615
                     /locus_tag="PLR93_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH26909.1"
                     /translation="MTLPDQKRRVTIKDVAYAAGVSTQTVSRVINNVSYVSEGTRKRV
                     ENVVEKMGYRPSTLARSLIQKRSYTLGVVTFGLKYIGPSRTLNGIADQADKLGYMLLM
                     KELDNFDKHSLNEVMDSLLARQVDGIIWVAPEIGDNHAFVDENADGGMPVPILFLAMR
                     PRTEIDSVATDNYQGGVMATKHLLEIGRKRIGHISGPMTWWEAEERKRGWRDTLENAG
                     LQVSEKFCAEGNWSSASGEQAFHQLLENYPDMDAVFVGNDQMALSAMREAHRLGIRIP
                     EQLAFIGFDNLPEAAYFYPSLTTVSQDLQLLGEESVQRIVEMIQARQNNQVLSAEARF
                     IQPTLIVRESTQMV"
     gene            2776..>3229
                     /locus_tag="PLR93_07820"
     CDS             2776..>3229
                     /locus_tag="PLR93_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH26910.1"
                     /translation="MLNDPGPVPHLFFTPIKTHPIKEKKMSKKLLYNLLAVLVLLSLV
                     LAGCGGGSSAGGDQKLVGISMPTKTSTRWISDGESMVKSFEELGYATDLQFADNDIPN
                     QIAQIENMVTKGADVLVIAAIDGSTLSDVLAKAKEAGVLVIAYDRLISK"
BASE COUNT          901 a          768 c          718 g          842 t
ORIGIN      
        1 gtatctacct gagacgtctc ctgcttgcca ccgccacatg ccgccaacag catggaaagg
       61 ataacgaaca ggctgaatag catgaaaatc tttttgttca tgattctcct ccagggaaaa
      121 tgatttggat acgacaacaa ccaaatggat actcttcgcc gcacctcctt atggatgatt
      181 tttacatccg cacgattcac ggataaccaa ctctgtacgc atcaaaacca gactttccgc
      241 ctgcccatcg tccattaggc ttttcaattg gcggacagct tctctaccaa cttcttcaat
      301 cggtgcacga acggtcgtca acgcggggga aatgtatcgg gaaaactgaa catcgtcgaa
      361 accgacaaca gccacatcgt cgggaatgag gcgcttggat gcttttaagg cacggtatac
      421 accaattgca gtgtcatcat cgcctgcaaa cacagcatca aattgaatcc cctcttgaat
      481 cattttttga atattgttaa atgcgatctc ttcttcaaag ccacccatca cgacaagttc
      541 tggtgcaaaa ggcaagttat gtttttctaa tgcttcgcga taaccgcgct cacgccaaag
      601 agaatcttca tgcccctctg gtccgcgcat ataaacgatt cgacggcgac catgcttttc
      661 gatcaggtga ctgaccagca tttcagcgcc gtctttgttt tcaatcgtaa ccacaggcat
      721 atccaaccca tcaggcggag tttgatgcat gagcactaaa ggaaatttga tcttatgcaa
      781 ccgttcaagt tcttcagctg caaggctaga ggtaaagacc aacaacccat cggtattatg
      841 ttcccccaat gtttttcgtt gacttgcatg tgcgccctgt gtggaatgaa tcaacaattc
      901 atatcccatt gcgcgcgttt cagattcaat gcccttcaac agcaaaggaa aaaatgcgcc
      961 accaatttca gaaagaatca aaccaatcgt attggtcttg cggctggcaa gagtccgcgc
     1021 cgccgtgcgt gggacaaaac gcaaatcatc cattgccgtg cgcacccgct cggctttatc
     1081 tgcctgaaca ggcaccgaac cattcaacac acggctaacc gtggcaatgg aaacgcccgc
     1141 atgcttggca acatccttga tagtaataga agacagatta ttcttcatac tgttgaaagc
     1201 gttttctgaa aacgttttca acagtatagg cttttgaggt caaagagtca agggcggctc
     1261 acccaccagt tacgatcagg cacgcttact gggattgttg cctttactca aaacttaaaa
     1321 gtcatgcttg taaaaacata tcttcataca tacaatcaat ttatattaat tgttgaagaa
     1381 gtttatcttc gtttgaagat aatttttctt cacacaaaag ggtaatttgt acatcgattt
     1441 ccatgcggaa aagggtaccc gtatggatca taaattttgc ctcttgactt agcccttcaa
     1501 ttttgttatt atgttatcga taacatgtta tcgataacat atatcagtat gtcataaggt
     1561 tattaccctt gaccctaccc gatcagaaga ggcgtgtcac aataaaggat gtagcatatg
     1621 ctgctggcgt atctacacag accgtttcgc gtgttatcaa taatgtttcg tatgtttcag
     1681 aggggactcg aaaacgagtg gaaaatgtag ttgaaaagat gggctaccgc cctagtacgc
     1741 ttgctcgtag ccttatccaa aaacgaagct ataccttggg agtagttaca tttggattga
     1801 aatacattgg tccatctcgc actcttaatg gaattgcaga ccaggcagac aaattagggt
     1861 atatgctgtt gatgaaagaa cttgataatt tcgacaagca tagtctcaat gaagtgatgg
     1921 actcgttgct cgcgcgtcaa gtcgatggaa ttatttgggt tgcacctgaa attggcgata
     1981 accatgcttt tgtagatgag aatgctgatg gtggcatgcc ggttcccatt cttttcttag
     2041 ccatgagacc tcgtacagaa atcgacagtg ttgcaaccga taattatcaa ggcggcgtga
     2101 tggcgaccaa gcacctgctg gaaatcggca gaaaacgcat cggtcatatt tctggtccca
     2161 tgacttggtg ggaagcagaa gaacgaaagc gcggctggag agacaccctt gaaaatgccg
     2221 gtttgcaagt ttctgaaaag ttttgcgcag aaggaaattg gtcttctgca agtggcgaac
     2281 aagcctttca tcaacttcta gagaactatc ccgacatgga tgcggtcttt gttggaaatg
     2341 atcaaatggc attaagtgcc atgcgcgaag ctcatcggtt aggaattcgc atccctgaac
     2401 aattggcttt tatcggtttc gacaacttac cagaagcggc atatttttac ccttctctaa
     2461 caacggtttc acaagatttg caattgcttg gtgaagaatc agtgcaaaga attgttgaaa
     2521 tgatccaggc aaggcagaac aatcaggtgt tatcggctga agcaaggttt attcaaccaa
     2581 ctctgatcgt ccgtgaaagt acgcagatgg tctgagcgtt tccgtccttc actattctga
     2641 aattctttgt tgaacgcgaa gagcacggaa aaaagaagga ggtgcaattg caagaaagtt
     2701 acatacttag agcatatgga agaacacatc acgtattggc gtatggtgtg cctcttccag
     2761 acaggacaag ccaccatgct gaacgaccca ggacctgtcc cccatttatt ttttacgcca
     2821 ataaaaacac atccaatcaa ggagaaaaaa atgtctaaga aattgcttta caacctgtta
     2881 gcagttctcg ttctgctcag ccttgtgctc gctggctgcg gcggcggcag cagcgcaggt
     2941 ggcgaccaga agttggtcgg catctccatg cccaccaaga cctctacccg ctggatctct
     3001 gacggcgaaa gcatggtcaa gtccttcgaa gaactcggct acgccaccga tctgcagttc
     3061 gctgacaatg acatcccgaa ccagatcgcc cagatcgaaa acatggtcac caagggcgct
     3121 gatgtgctcg tgatcgccgc cattgacggt tcaaccctct ctgacgtgct tgccaaggcg
     3181 aaggaagctg gcgtgctcgt gatcgcttat gaccgcttga tctccaaga
//
LOCUS       DAOQFT010000276         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17484_length_3223_cov_8.120581, whole genome shotgun sequence.
ACCESSION   DAOQFT010000276 DAOQFT010000000
VERSION     DAOQFT010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17484_length_3223_cov_8.120581"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /gene="panC"
                     /locus_tag="PLR93_07825"
     CDS             complement(<1..471)
                     /gene="panC"
                     /locus_tag="PLR93_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNH26911.1"
                     /translation="MKIVSSLSDLRAARLSLDGTVGLVPTMGYLHEGHLSLVSRAKAE
                     NQHAIVTIFVNPTQFGPNEDLSKYPRDLERDLDLLRPLGVDVVWTPTAEVMYPPGYQT
                     WVEVEALTSPLEGAMRPGHFRGVTTVVAKLFNVTQPTKAYFGQKDAQQAAVIRRM"
     gene            573..794
                     /locus_tag="PLR93_07830"
     CDS             573..794
                     /locus_tag="PLR93_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26912.1"
                     /translation="MAFGYTNSKGTTYYLHARGKMFFFAKEVKEGALDAVPAGYNVVE
                     MKTGMPVLKKAVAEGSATTTETTNTESAG"
     gene            926..1324
                     /locus_tag="PLR93_07835"
     CDS             926..1324
                     /locus_tag="PLR93_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNH26913.1"
                     /translation="MPALNRVQLIGRLGKDPESKYTPTGKKVCHFSLAVSNRWKDKNG
                     ETKETTEWVNIETWGRLGEVCQEYLKKGSLIFVEGRLKTDKYEDRESGETRYYTKIIA
                     QSLQFLDRKASEEPVMAVEEESGEYELAIE"
     gene            1424..2902
                     /locus_tag="PLR93_07840"
     CDS             1424..2902
                     /locus_tag="PLR93_07840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH26914.1"
                     /translation="MPLPEKIGRYEVKSELGRGGMATVYRAVDPNSGREVAVKVLPRE
                     MMHDPQFRERFEREIKMIASLEHPAIVPVYDVGDIDGQPYFVMRFMTGGSLSDLIEKG
                     SIPIDETAKIIEKIAQGLAYAHRKGVIHRDLKPDNILFNDDGEPFISDFGVAKLSESS
                     SNLTGSGVIGTPAYMSPEQAQGNEIDQRSDVYGLGVIVYQMLSGHQPYSADTPMGVVV
                     KHITEPVPEILKVLPNLPEDVDAIIKTAMAKDKTQRYANTIELAKALNTVAFGTPGDI
                     TFHTMNGRTRLPSNSKPLSRGQTTGLAVGGVILLLAIVGVFLLRNQLFASSSPTSTTD
                     AASTEVSETSTPVLVVVTATLPEATLASGPASAPACSANVSFPAPIGKETNSFCTQKI
                     PYAYANMADHATFTILDSSTAGAKCRQEAIKNGEKVVSCTGPSFAVIDLEVCEPVELK
                     PEELSQCSADSTYNAANQCCVQQPPQKASCKVIKIQLKGCGG"
     gene            2956..>3223
                     /locus_tag="PLR93_07845"
     CDS             2956..>3223
                     /locus_tag="PLR93_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNH26915.1"
                     /translation="MGTLYLVATPIGNLEDMSPRAVRILREAILIAAEDTRHTGTLLK
                     HFNVETPLTSYFEHNKLTKQDFILEKLAQGDVALVSDAGTPAIND"
BASE COUNT          894 a          818 c          765 g          746 t
ORIGIN      
        1 catgcgtcga ataactgccg cctgttgcgc atccttttga ccgaagtatg ccttcgtcgg
       61 ctgggtgaca ttgaacagct tcgccaccac cgtcgtcacc ccacggaagt gacctgggcg
      121 catcgcccct tcaagcggac ttgtcaacgc ttccacctct acccaggtct ggtagccagg
      181 cggatacatt acctccgccg tgggcgtcca aaccacgtcc acaccgaggg ggcgcagaag
      241 gtctaagtca cgttcgagat cacgcggata tttcgagagg tcttcgttcg gtccgaactg
      301 ggtagggttc acaaaaatag tgacgatggc atgctggttc tcggctttgg cacgagagac
      361 aagcgataaa tgcccctcgt gaagataccc catcgtggga acaagaccaa ccgtcccgtc
      421 gagagagagg cgagcagctc ttaggtcaga gagagaggag acaattttca agtcaggctc
      481 caatttgtct atacatttac aaaagtacta cttgacaggc tagaaatttt gttctacact
      541 tttatcagaa tttagacaaa aggagaaata caatggcttt cggatacacc aactcaaaag
      601 gaactaccta ctaccttcac gctcgcggca agatgttctt cttcgctaaa gaggttaagg
      661 aaggcgctct cgatgccgta cccgctggtt acaatgtggt cgaaatgaag accggcatgc
      721 ccgtcctcaa gaaggcagtt gcagaaggca gcgccaccac cacagaaacc accaacacgg
      781 aaagtgcagg gtaattcaag tataaccctg ttcgacaacc tggcttttgc cgggaaaata
      841 tcaacctgtt cttttagata aaaaggagac taattatgac acgtacacag catctaaaag
      901 ccactcaaac caaaggggtg cagccatgcc agcattaaac cgcgttcagt tgatcggtcg
      961 tttggggaaa gacccagaaa gtaaatacac ccccactggc aagaaggtct gtcacttcag
     1021 cctcgccgtc agcaaccgtt ggaaagacaa gaacggcgag accaaagaaa ccaccgaatg
     1081 ggtcaacatc gaaacatggg gacggctggg cgaagtttgc caagaatatt tgaaaaaagg
     1141 cagcttgatc ttcgtagaag gtcgcttgaa aaccgataag tacgaagatc gggaaagcgg
     1201 cgaaacaagg tactacacca agatcattgc ccaaagcctg caattcctcg atcgaaaagc
     1261 aagtgaagag cctgttatgg cggtcgaaga agaatctggc gagtatgaac ttgccataga
     1321 gtaataaatc aataaaaaga caactgccaa atggcagttg tctttttatt acaaatgggt
     1381 gaatcttctt gcgcccctgt tttggctggt ttatacttga actatgccac ttccagaaaa
     1441 aatcggtcga tacgaagtta aatcagaact cggacgcggc ggtatggcta cggtctaccg
     1501 cgccgttgac cccaactcgg ggcgcgaggt tgccgttaag gtcttgccgc gtgaaatgat
     1561 gcacgacccg caattccgcg aacgttttga acgcgagatc aaaatgatcg cctcattgga
     1621 acatccagcc attgtgcctg tgtatgatgt aggcgatatt gacggtcagc cttattttgt
     1681 catgcgcttc atgacgggcg gctccctttc cgatctcatc gaaaaaggca gtattccgat
     1741 tgatgagaca gcaaagatca tcgaaaagat cgctcagggc ttggcatatg cccatcgcaa
     1801 aggtgttatc catcgcgatc tgaagccaga caatattctc tttaatgacg atggcgagcc
     1861 gttcatctcc gactttggtg ttgccaaatt atctgagtca agcagtaatc taaccggtag
     1921 cggtgtgatc ggtactcctg cctatatgag tcctgaacag gctcagggga atgaaatcga
     1981 tcaacgcagt gatgtgtatg gcttgggcgt gatcgtctac caaatgttga gcggacatca
     2041 gccttacagc gccgataccc ccatgggtgt ggtcgtcaag cacatcaccg agccggtgcc
     2101 cgaaattctt aaagtacttc ccaatctccc tgaagatgta gatgccatca tcaaaacagc
     2161 gatggcaaaa gacaagactc aacgttacgc caacaccatt gaattggcaa aggcgttgaa
     2221 taccgtggca tttggtacgc cgggcgatat taccttccac accatgaatg gacgtacacg
     2281 tctgccttcc aattcaaagc ccttatcgcg cgggcaaacc actggcttgg cagtcggcgg
     2341 tgtgattctg cttttagcca tcgttggagt gtttctgctc cgcaatcagc tatttgcatc
     2401 cagctcgcca acctctacaa cagatgcagc ctcaacagaa gtcagtgaaa cttcaacacc
     2461 ggtcttggtt gtagtcaccg ctaccctgcc cgaagcgaca ttagcttctg gtcccgcctc
     2521 ggcacccgct tgttctgcca atgtttcctt ccctgcacct attgggaaag aaaccaatag
     2581 tttctgtaca cagaagattc cgtatgccta tgccaatatg gcagatcatg caacgtttac
     2641 aatattagat tccagcacag ctggtgcgaa atgtcgtcag gaagcgatta agaacggcga
     2701 aaaggtcgtt tcgtgtactg gtcccagttt cgcagtgatc gaccttgaag tctgtgaacc
     2761 cgttgaatta aaacctgaag aactttctca atgctcagca gacagcacct acaatgcagc
     2821 caatcaatgc tgtgttcaac agccgcccca aaaagcatca tgtaaagtaa taaagatcca
     2881 gcttaagggc tgcggcggat aaacaaaatc gggcgagcaa tcgcccgatt tttatttcgt
     2941 gagataatta tttgcatggg aactttatat cttgtcgcca ctccaattgg aaatcttgaa
     3001 gatatgagtc cgcgtgcggt gcggattctt cgcgaagcaa ttctgattgc cgctgaagat
     3061 acacgtcaca ccggcacctt gctcaagcac ttcaatgttg aaacacctct caccagttat
     3121 ttcgaacata ataaactcac caagcaagat ttcattcttg aaaaattagc acaaggcgac
     3181 gtagccctcg tttccgatgc agggacgccc gccattaatg acc
//
LOCUS       DAOQFT010000277         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17522_length_3218_cov_5.175466, whole genome shotgun sequence.
ACCESSION   DAOQFT010000277 DAOQFT010000000
VERSION     DAOQFT010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17522_length_3218_cov_5.175466"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLR93_07850"
     CDS             complement(<1..940)
                     /locus_tag="PLR93_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNH26916.1"
                     /translation="MDTKSLLKLLVETESPSHEKSAVDRVGAIVADEARKLGAEIEIF
                     SNKETGDHVIARWRGEGKPILLVCHMDTVFPLGTIEKFPYQEEEGKIKGPGVSDMKAS
                     IAISLGAIEEAYNNGAKRAITLLCTSDEEIGSITSRELIQNMAKEAELVFVMEPALLD
                     GSLKTWRKGGGGFKVKVHGRAAHAGGDHEKGRNAIEEMAHQVLAIQKMTDYSKKTTLN
                     VGVIHGGTVSNVVPDYAEIDIDVRILQPEEWGRVEGMMMALKPVLDGTTVEVTGSLNR
                     GPMPFDDLMQTTFGKAKSIAAEIGIDLKAGGTGGVSD"
     gene            complement(962..1774)
                     /gene="uppP"
                     /locus_tag="PLR93_07855"
     CDS             complement(962..1774)
                     /gene="uppP"
                     /locus_tag="PLR93_07855"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNH26917.1"
                     /translation="MNIFHAIVLGIVQGLTEFIPVSSTAHLFIVSNLLGLPSDDQVFA
                     FDVIIQLGTVGALLLFFWHDYWDIAKAVFEGIKNRKPFEDYHSRLGWLVIVATIPAGV
                     VGLALKPLVQQMSGDPLLWGGIRLLFTSALLAAVEYFDKKSRTLESATWVDALSVGLF
                     QVLAIIPGASRSGSTMAGAMFRGFDRPSAARFAFLMSAPILLAAGLYETIQVILLPNT
                     TEFLPILLTGFLVSAIVGWLAIRWLMGYLRKHSLYVFSVYCLIVGVIVLALK"
     gene            complement(1775..2980)
                     /gene="tgt"
                     /locus_tag="PLR93_07860"
     CDS             complement(1775..2980)
                     /gene="tgt"
                     /locus_tag="PLR93_07860"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNH26918.1"
                     /translation="MPEKEPIEGQFAYQFGDTLSSCIICPMFDFSITAKNNRARTGVF
                     STPHGDLITPVFAPVGTQATVKTLTPEHLKSINASLVLSNTYHLYLRPGDELVRDMGG
                     LHKFMQWHNPMLTDSGGFQVFSLSQTRKVDEDGVTFKSHIDGSTHRFTPEKSIAIQEN
                     LGADIIMAFDECSDPNDHAYIKKAMERTHRWAERSLKAKTRSDQALFGIVQGGVDPTL
                     RAESAKFIASLETPGIAIGGLSVGETKQEMHDTLDVVTPLLPENKPRYLMGVGTPEDL
                     INGVLRGVDIFDCVLPTRLARHHSAFAPEGRLNMMNASFARDERPIDETCDCYACQTF
                     TRAYIRHLIVAKEMLASTLVSIHNLRALIRLMENIRVYIAEGSFEDRAPELLLQWGNN
                     AKRVVNGVK"
     gene            3032..>3218
                     /locus_tag="PLR93_07865"
     CDS             3032..>3218
                     /locus_tag="PLR93_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26919.1"
                     /translation="MTKPRLTHSLFLILLLTACAAPATAAPTQTSTPKAPEATSTIDN
                     STPTLTVTPDAKLATVEG"
BASE COUNT          928 a          862 c          659 g          769 t
ORIGIN      
        1 catcagagac gccgcctgta ccacccgctt tgaggtcaat gccgatctca gcagcgatcg
       61 acttggcttt tccaaatgtc gtctgcatca aatcatcgaa aggcataggt ccgcggttga
      121 gagagccggt tacttctacc gtagttccgt ctaataccgg tttcagagcc atcatcattc
      181 cttccacacg cccccactct tccggttgca aaatgcggac gtcaatatca atttcagcat
      241 aatcaggtac aacattggaa accgtcccac catggatgac gccaacattc aaggtagtct
      301 ttttggaata atccgtcatc ttttgaatgg caagtacttg atgtgccatt tcttcaatcg
      361 cattccgccc tttttcatgg tcaccgcctg catgcgcagc ccgtccatga accttgacct
      421 taaatccgcc gcccccttta cgccaagtct tgagagaacc atccagcaaa gcaggttcca
      481 tcacaaaaac gagttcagct tcctttgcca tgttttgaat tagttcgcga gatgtaatac
      541 tgccgatctc ttcatctgaa gtacacagca acgtaatagc tcgttttgct ccattgttat
      601 atgcctcttc gattgcaccg agagaaatag caatgctggc tttcatatca gagacgcccg
      661 gtcctttgat cttgccttct tcctcttgat acggaaattt ttcaatcgtc cctaatggaa
      721 aaactgtatc catatgacag accaacaaaa tgggttttcc ttcccctctc caacgtgcaa
      781 tcacatggtc acctgtctct ttgttcgaaa aaatctcaat ctccgcacca agtttacgag
      841 cttcatctgc cacaatagca ccgacacggt ccacggcaga tttttcgtgc gagggagatt
      901 ctgtttctac aagaagtttt agaagagact tggtatccat ggtcagctcc aattcaaaat
      961 tttatttcaa cgccaaaaca atcacaccaa caatcaaaca ataaacagaa aagacataca
     1021 aagaatgctt tcgcaaatat cccatcaacc agcgaatagc aagccagccc acaatagcag
     1081 aaaccaaaaa acctgtaagc aaaataggca aaaattcagt ggtgtttggt aggagtataa
     1141 cttggatcgt ttcatacaag cctgcagcca acagaatggg tgctgacatg aggaaagcaa
     1201 agcgcgccgc agagggtcga tcaaagccac ggaacatggc acctgccatt gtcgagccgg
     1261 aacgggaggc gccgggaatg attgctaata cctgaaatag cccaaccgaa agagcgtcca
     1321 cccaagttgc tgattcaagc gtacgactct ttttatcaaa atattcgact gccgccaata
     1381 acgctgaagt gaataacaat cgtattccgc cccataacaa tgggtcgcca gacatttgtt
     1441 gaaccaacgg cttaagcgcc aaccccacca ccccagcagg aatagttgct acaatcacca
     1501 accaccccaa acgagaatga tagtcttcaa agggttttcg gtttttgata ccttcaaaaa
     1561 cagctttggc tatatcccaa taatcatgcc aaaagaatag aagcaatgct cctactgttc
     1621 ctaattgaat aatcacatca aatgcaaata cttggtcatc tgacggaaga ccaagtaaat
     1681 tgctcacaat aaacaagtga gccgtagaag aaacagggat gaattcggtc aacccttgta
     1741 caatccccaa aacaattgca tgaaaaatat tcaattactt aactccgtta actactcgct
     1801 tcgcattatt cccccactgc aacaacaact ctggcgcacg atcttcaaat gagccctccg
     1861 caatataaac gcggatattc tccattaagc ggattaacgc ccgcaaatta tggatggaaa
     1921 ccaaagtact ggcaagcatt tcctttgcca caatcaagtg acgaatgtac gcccgtgtaa
     1981 aagtctgaca agcataacag tcacaggttt catcaatagg gcgttcatcg cgcgcaaagg
     2041 aagcattcat catattaagg cgaccttccg gtgcaaaagc agaatgatgg cgcgccaaac
     2101 gggttggcaa aacacaatcg aaaatatcca cgccccgcaa gacaccgttg atgaggtctt
     2161 caggtgtgcc aacgcccatt agatagcgcg gcttattctc aggcaacaac ggtgtgacca
     2221 catccagcgt atcgtgcatc tcttgcttgg tttcaccgac agataaacca ccaatcgcaa
     2281 tgccaggcgt ctctaacgag gcgatgaact tggcagactc cgctcgcagc gtaggatcca
     2341 ctccaccttg gacgatcccg aacaaggctt ggtctgagcg cgtctttgct ttcagcgaac
     2401 gctccgccca acgatgagtc cgctccatgg ctttcttgat atatgcatgg tcgttcgggt
     2461 cagaacattc atcaaatgcc atgatgatgt cggcgccgag attctcttga atcgcaatcg
     2521 acttctctgg cgtaaaacga tgcgtagacc catcgatatg actcttgaag gtcacgccat
     2581 cttcatccac tttgcgagtt tgcgaaagag aaaaaacctg aaagccaccc gaatcagtaa
     2641 gcatggggtt atgccactgc atgaatttat gcagcccgcc catatcgcgc accaattcat
     2701 cgccagggcg cagatacaaa tgataggtat tgctcaagac gagtgaagcg ttaatgctct
     2761 tgagatgttc aggcgtcagt gttttgacag tggcttgtgt acccactggt gcaaacacag
     2821 gagtaatgag atcgccgtgt ggcgtggaga aaaccccggt gcgggcgcgg ttatttttag
     2881 ctgtaatgct gaaatcaaac atggggcaaa ttatacagga tgaaagtgta tcaccgaatt
     2941 ggtatgcaaa ctgcccctca attggctctt tttcaggcat tgaccgtttc atataattgc
     3001 aaccatagac tcattcttag agagagaaat catgacaaaa ccccgcctca cccatagcct
     3061 atttttgatc ctgttgctga cggcttgtgc cgcgcccgca actgccgctc ccacacaaac
     3121 ctccacccct aaagcgcccg aagcaacctc caccatcgac aactccacac ctacattgac
     3181 agtcactccc gatgcgaagc ttgctaccgt agaaggga
//
LOCUS       DAOQFT010000278         3217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17533_length_3217_cov_3.881720, whole genome shotgun sequence.
ACCESSION   DAOQFT010000278 DAOQFT010000000
VERSION     DAOQFT010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17533_length_3217_cov_3.881720"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLR93_07870"
     CDS             complement(<1..514)
                     /locus_tag="PLR93_07870"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26920.1"
                     /translation="MNKNIEEPVEPIQPILSSTETRRGGETTFVAMRHRNYQLYFGGQ
                     LISNIGTWMQVIAQAWVVYQLGHSELTLGLVAFASAIPNLVVSPWGGVIVDRISRRKV
                     LMLTQSGAMLLSFILAWLTFTNIVQEWHVILLAALTGVITAFDAPARQAITPELVGKK
                     DVANAIAMNSM"
     gene            complement(537..977)
                     /locus_tag="PLR93_07875"
     CDS             complement(537..977)
                     /locus_tag="PLR93_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH26921.1"
                     /translation="MSTSNQLREQALDGFWETIPSLWNFVRSYIRATATSKFDITVEQ
                     FHVLRFVRKGMSISELAIAKKVSRPAISQAVDVLVKKGLLTRVQNTKDRRYVTLALSN
                     EGNALLDTVFKETRAWMKGHMSKLTNEELESIAKAMQAFKKILP"
     gene            1211..>3217
                     /locus_tag="PLR93_07880"
     CDS             1211..>3217
                     /locus_tag="PLR93_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH26922.1"
                     /translation="MPNQLIHETSPYLIQHVNNPVNWYPWGEEALTKARAENKPIFLS
                     IGYAACHWCHVMAHESFENAETAAFMNEHFINIKVDREERPDLDNIYMQATVAMTGSG
                     GWPMSVFLTPDLKPFYAGTYFPPVRRYNMPSFKEVLNGLSNAWQNDRDEVEKTGIKVR
                     DHLQTQVNLQSSELLVPEHLTAIAKTMQESYDWGYGGWGDAPKFPQPMAVEFLLHHSS
                     AQKTEEYTKLIKHYLYAMTRGGMYDVVGGGFSRYSTDNQWRVPHFEKMLYDNALLTRA
                     YLHAWQVTRDPFYKRIVEETLTFIEKEMTHPQGGFYSSLDADSEGEEGKFYVWTLDEI
                     RGILKDDSAFFEAAYGISSKGNWEGKTVLQRAMDDSTLAARFQLDPQTVPSKLAQSHS
                     RLYSARAQRTRPGTDDKILTAWNGLMLAAIAEAARILNGESGRLLSPDSEQQAVRLHK
                     MAVQNADFILTNLRPNGQLRRAWRDGKTTNAVFLEDYAALVLGLIELYQTDFNERWFT
                     AARELTDEMIEKFQDAEGGFFDTPSDGENLLFRPKDVQDNATPSGNALACEALIKLAA
                     YTDEGRYRDLAEKSLGLVTRFALRYPLGFARWLSAAQLASGTLKQVAVLGEAGDPDFQ
                     RLLNVIRSEYRPSVVVAASSYPISKNAPALLNERALMEGKATAYV"
BASE COUNT          831 a          806 c          783 g          797 t
ORIGIN      
        1 tcatcgaatt catcgcaatg gcattggcaa cgtctttctt gccaaccaat tccggggtga
       61 tggcttgacg agcgggtgca tcaaaggcag tgatcacgcc ggtcagcgcc gccaaaagaa
      121 ttacatgcca ttcctgaacg atattggtaa aggtcaacca ggcaaggatg aaggacaaca
      181 gcatcgcacc agattgagtc agcatcagca ccttgcggcg cgatatacgg tccacgatca
      241 cgccacccca gggagagaca accaaatttg ggatggcgga ggcaaaggca accagaccca
      301 aagtcagctc ggaatgaccg agttgataca ccacccaagc ctgtgcaatg acctgcatcc
      361 acgtgccgat gttggagatg agttgcccgc caaagtacaa ctgataatta cggtgccgca
      421 tggcaacgaa ggtagtttcc ccgcctctac gtgtctctgt gctcgaaagg ataggctgaa
      481 taggctctac cggttcttcg atgtttttat tcattcttga cccaaactcc taaaactcat
      541 ggcaatattt ttttgaaggc ttgcattgcc ttggcaatcg actccaactc ttcgttggtc
      601 agtttgctca tgtgaccttt catccatgca cgagtttctt tgaagaccgt atccaaaagc
      661 gcgttgcctt cattgctcaa ggcaagggtc acatagcggc ggtctttcgt gttttgcacg
      721 cgggtcaaca gtcccttttt gaccagcaca tccactgcct gactgatggc ggggcggctg
      781 acttttttgg caattgccaa ttcgctgatc gacatgcctt tgcgtacaaa acgcagcacg
      841 tgaaattgtt caacagtgat gtcgaatttg cttgtggctg tggcgcggat gtatgaccgc
      901 acaaaattcc aaagggaagg gatcgtttcc caaaagccgt ccaaggcttg ttcgcgtaat
      961 tgattggatg tgctcataaa acctttcaaa atgtgtaact ctattaatag ttaagtattg
     1021 aaactatttt atttttacat tttggagatg tcaaggggag ttggctcatt ggtcgaatct
     1081 ctcccagatt gcttgaatgc caccttgaga attgaacatt tttaaaactg ccctggatta
     1141 agggcatacc caattgcttc gactataata aaatatcaaa ttgtttcaaa atttagtata
     1201 taggtgcccc atgcccaacc agctaatcca tgagacttca ccctatctca tacaacatgt
     1261 gaataatcct gttaattggt atccttgggg ggaagaggcg ctaacaaaag caagggcaga
     1321 aaacaagccc attttcctta gcatcggcta tgctgcatgt cactggtgtc atgtcatggc
     1381 tcacgagtca ttcgaaaatg cagaaactgc cgcatttatg aatgaacact ttatcaacat
     1441 caaagtggat cgggaagaac gccccgactt agacaatatt tacatgcagg caaccgtcgc
     1501 catgaccggc tccggcggct ggcccatgtc ggtctttctc acaccagacc tcaaaccgtt
     1561 ttatgctggc acgtattttc cacctgtgcg ccgctacaac atgccctctt tcaaggaggt
     1621 gcttaatggc ctttccaacg cctggcaaaa tgaccgtgat gaagtggaaa agaccggcat
     1681 caaagtccgt gaccatttgc aaacgcaggt gaatcttcaa agcagtgaac tgctggttcc
     1741 cgaacatttg acagccattg ccaaaaccat gcaggaatca tacgactggg gttatggtgg
     1801 ttggggcgat gcaccaaaat tcccgcagcc gatggctgtg gagtttcttc tgcatcactc
     1861 gtctgcacaa aaaacagaag aatacaccaa actgatcaag cattatctat acgccatgac
     1921 gcgtggcgga atgtacgatg tagttggagg cggcttttct cgctatagca ccgacaatca
     1981 atggcgcgta ccccattttg aaaaaatgtt gtacgacaat gccttgctga cccgcgccta
     2041 tcttcacgcc tggcaggtta cacgtgatcc gttctataaa cgcattgttg aggagacact
     2101 gaccttcatt gaaaaagaaa tgacacaccc acaaggcggc ttctattcat cgcttgatgc
     2161 agactcagaa ggtgaagagg gcaaatttta tgtttggact ctggatgaga tacgtggcat
     2221 tctgaaagac gactctgcct tttttgaagc ggcatatggc atcagcagca aggggaattg
     2281 ggaagggaaa acggttcttc aacgcgcaat ggatgactca actcttgcag cccgcttcca
     2341 acttgacccg caaactgttc catcaaaact ggcccaatct cattccaggc tttattctgc
     2401 ccgtgcccaa cgcacccgcc ctggcactga tgacaagatt ctgactgcat ggaacggctt
     2461 gatgcttgcc gccattgccg aagctgcccg aattttgaat ggggaatccg ggagattact
     2521 ctcgccggat tccgagcaac aggctgttcg gctccataaa atggcggtgc aaaacgcaga
     2581 ttttatactc accaacctgc ggcccaatgg ccaactgcgt cgcgcatggc gggatggcaa
     2641 aaccacaaat gcagtctttc tcgaagatta tgccgccctc gtcctcggtt tgattgagtt
     2701 gtatcagact gatttcaatg aacgttggtt taccgccgcc agggaattaa ccgacgaaat
     2761 gatcgaaaaa ttccaagatg ccgaaggcgg tttctttgat acgcccagtg atggggagaa
     2821 tcttttattc cgcccaaaag acgtacaaga caatgccaca ccctcaggca atgcattggc
     2881 ctgtgaagcg ctcatcaaac tcgctgcgta tacagatgaa gggcgatacc gtgatctggc
     2941 ggagaaatcc cttggcctgg tgacacgctt cgccttgcga tatccgcttg ggtttgcccg
     3001 ctggttgtca gcggcgcaac tggcttcggg gacgttgaag caggttgcgg tgttgggtga
     3061 ggcgggagac ccggacttcc agcgtttgct caacgtgatt cgaagcgaat accgacccag
     3121 tgtggtggta gctgcttcgt cttatcccat ttcaaaaaac gcgcctgcgc ttttgaacga
     3181 acgcgctttg atggaaggca aagcgacagc gtatgtg
//
LOCUS       DAOQFT010000279         3206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17639_length_3206_cov_6.673754, whole genome shotgun sequence.
ACCESSION   DAOQFT010000279 DAOQFT010000000
VERSION     DAOQFT010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17639_length_3206_cov_6.673754"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLR93_07885"
     CDS             <1..899
                     /locus_tag="PLR93_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNH26923.1"
                     /translation="VVVNSGTGGGGSEGLFPTILRKLQIKTNKPERPRYDPELYRVVR
                     GDSMYRIAEDFKIKSETILYVNDQLEDNPHSLRPGMELTIPPADGLYYTWKEGDTFES
                     VAEEYNANADDIINFTGNQIDLTDPKVEVGTQIFIPGGSRELVNWAAQLPTNARGVNT
                     GTGGGNAANVCGGGPVASGFGWPADDHTLSGNAYGPGHLGIDISAPEGANVYAAGTGV
                     VTMAAGGWNYGYGNVVQIDHGNGYVTVYAHLSSILVSQCQTVGQGSVIGLAGNTGNSF
                     GAHLHFEIRVGGSNINPYDIVQ"
     gene            946..1725
                     /locus_tag="PLR93_07890"
     CDS             946..1725
                     /locus_tag="PLR93_07890"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNH26924.1"
                     /translation="MNESSLKKRLAKLNLGGLRYFESLGSTNDEALAWAARGADDFSL
                     VVADEQTQGRGRLDRKWFTPKGSAIAMSLILRPTAVMRSHLSRTVGLAALSLADACLK
                     LGLSPKIKWPNDILLDSKKTAGILVEMVWAGEDADSLVIGMGINVHKSAVPPRETLQF
                     PATSLEDMLGNAPKREDIIFQILSNVQRWREQIGTERFIQAWEEKLAFRGEAIQIHAG
                     GAESHQGILDGLESDGSLRICDEHNKSVIVRFGDVSLRPTA"
     gene            1756..1980
                     /locus_tag="PLR93_07895"
     CDS             1756..1980
                     /locus_tag="PLR93_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26925.1"
                     /translation="MFDDLRNDAAKQYEDEVQAEYQPASVTSSGAPRQRSTKFLGMTS
                     IQRFILVFLLMTATCVIGFLCLFAFGKIAF"
     gene            2032..2796
                     /locus_tag="PLR93_07900"
     CDS             2032..2796
                     /locus_tag="PLR93_07900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH26926.1"
                     /translation="MQILNTLNFFTSKNLFKSYKRLGFWQTIILFSGVAFVSVTGIIV
                     PTNMLLGGSIRTGMVINFIVCTTFMPYHLHQILSLMVELDTLRMNIYDKSIRDELTKA
                     YNRRYFFEASKAIENNEALIPQNTSLLLIDIDDFKLLNDTYGHDIGDLALKTLTEESS
                     RLLRTTDVFARYGGDEFICLLPNTKQSQAYEIATRLLESVNKHPFYKKDGNIFLRLSI
                     GIATTTSETKLNDLISLADKALYKSKQNGKNTVEAL"
     gene            complement(2836..>3206)
                     /locus_tag="PLR93_07905"
     CDS             complement(2836..>3206)
                     /locus_tag="PLR93_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNH26927.1"
                     /translation="LLVVTTNGFGKQTPLKEYTPKGRATGGISTIDQKALQEIGKIAA
                     ARVVNKDDDLTIMTANGVAIRLKVKDVKQSGRATRGVHLIKPQEGDSVASVARISADD
                     LKKAGAQIEEQEAEPQPKLV"
BASE COUNT          828 a          764 c          771 g          843 t
ORIGIN      
        1 cagtggtggt caactcaggc acgggcggag gaggaagcga aggtctcttc cccaccattc
       61 tccgcaaatt gcagatcaag accaacaagc cggaacgtcc gcgctatgat ccagaacttt
      121 atcgcgttgt gcgcggcgac tcgatgtatc gcattgccga agatttcaag attaagtctg
      181 aaaccatttt gtacgtcaac gaccagttgg aagataaccc acacagtttg cgtcccggca
      241 tggagttaac cattcctccg gcagatggtt tgtattacac ctggaaagaa ggcgatacgt
      301 ttgaatctgt ggcggaagaa tataacgcca atgctgatga cattatcaac ttcacaggta
      361 atcagatcga cctgaccgac cccaaagtag aagttggcac ccaaattttt atccctggtg
      421 gttctcgtga gttggtgaac tgggcagcac aattgcccac caatgcacgc ggagtgaaca
      481 ccggcacagg cggtggcaat gccgccaatg tgtgtggcgg tggacctgta gcaagcggct
      541 ttggttggcc cgccgatgac cacaccctct ctggaaatgc ttacggacct ggtcaccttg
      601 gcattgacat cagcgcccct gaaggtgcca atgtgtatgc cgctggcacc ggtgttgtaa
      661 ccatggcggc tggcggttgg aattacggtt atggcaacgt agtgcagatc gatcacggca
      721 atggttacgt aacggtttat gcccacttaa gctccattct cgtcagtcaa tgtcaaaccg
      781 tggggcaagg ctcggtgatc gggctggcag gcaacacggg taactccttc ggcgcgcact
      841 tgcactttga aattcgtgtc ggtggttcga atatcaaccc gtacgatatt gttcaataaa
      901 atagagacct gacagtttca aagcctgtca ggtcttttaa tttccatgaa cgaatcctct
      961 ctcaaaaaac gacttgcaaa actaaacctc ggcggcttgc gctatttcga atcgctaggc
     1021 tctactaatg acgaagctct tgcatgggca gctcgtggcg cggatgattt ctctctcgtc
     1081 gtggcagatg aacaaactca agggcgtggg cggttggaca gaaaatggtt cacgcctaaa
     1141 ggcagtgcga ttgcgatgag tctcatccta cgccccactg cggtgatgcg ctcgcatctc
     1201 tcgcggaccg tgggcttggc agctctctca ttggcagatg cttgtctcaa gctgggtctg
     1261 tctcccaaga tcaagtggcc caatgatatt ctgctcgata gcaaaaagac tgcaggcatc
     1321 cttgttgaaa tggtgtgggc tggcgaagat gccgattcat tggtaattgg gatgggaatt
     1381 aacgtccata agtctgcagt gccgccaaga gagacgctcc aattccctgc aaccagtttg
     1441 gaagatatgc tgggaaacgc tccgaagcga gaagatatca tctttcagat tttgagcaat
     1501 gtgcaacgct ggcgtgaaca gatcggcaca gagcggttca ttcaggcgtg ggaggaaaaa
     1561 ttagcctttc gcggagaggc aattcaaatc cacgctggag gcgcggagtc ccatcagggt
     1621 atattggatg gtttggagtc tgatggtagt ttgcgcatct gtgatgaaca taacaaatct
     1681 gttattgtgc gctttgggga tgtgagtctg cgcccgactg catgatatac ttttaaaaaa
     1741 taatcagagg ttcccatgtt tgatgatctt cgaaatgatg ccgcaaaaca atatgaagac
     1801 gaagtacagg cagaatatca gccagcttca gtcacttcat cgggtgcacc gcgccaacgt
     1861 tcaaccaagt tcctcggcat gacatccata cagcgtttta tacttgtgtt cttattgatg
     1921 accgcaacct gtgtgattgg ttttttgtgc ctttttgcct ttggaaaaat cgctttctaa
     1981 gtcgcaacgc atttaacaat agggtgttta gtatctgcat agagtgatag catgcaaata
     2041 ctaaacaccc tcaatttttt tacaagcaaa aatcttttca aaagttataa acggctggga
     2101 ttctggcaaa cgatcattct tttttcgggg gttgctttcg tttccgtaac aggcattatc
     2161 gtccctacca acatgctctt gggcggaagt atcagaacag gcatggttat caacttcatt
     2221 gtttgtacaa ctttcatgcc gtaccacctt caccaaatcc ttagcctcat ggtggagcta
     2281 gacactttaa gaatgaacat atacgacaag tcgattcggg atgagttgac gaaggcatat
     2341 aaccgccgct acttttttga agcgtctaag gctatcgaga acaacgaagc cttaattccc
     2401 caaaatacat ctctgctatt aattgatatt gatgatttca aattgttaaa tgacacctac
     2461 gggcacgata ttggcgacct tgcgttaaag acgctcacag aagaaagctc gcgtctgttg
     2521 cgtactacag atgtctttgc acggtatggt ggagatgaat ttatctgcct gttgcccaac
     2581 acaaagcaat ctcaagccta tgagatcgca acccgcctgc tcgaaagcgt gaacaaacat
     2641 cctttctaca aaaaggatgg gaacattttt ctccggctta gtattgggat tgccacaaca
     2701 acaagcgaga ccaaacttaa tgacctaatc tctttagccg ataaagcttt atataaatca
     2761 aaacaaaacg gaaagaatac tgtagaagcc ttataaaaaa agacctagtg agaaatcacc
     2821 aggtctcttt tatcgttata ccaacttcgg ctgtggttca gcttcttgct cttcgatttg
     2881 agcaccagcc tttttcagat catcggctga gatgcgtgcc accgacgcca cactatcacc
     2941 ttcttgtggc ttgatgagat gcacgccgcg tgttgctcgc ccagattgtt tcacatcttt
     3001 aaccttcaaa cgaatcgcta caccattggc ggtcataatg gtcaggtcgt catctttgtt
     3061 gaccacacgt gccgctgcga tcttgccgat ctcttgaagc gctttctggt caatggtgga
     3121 aataccgccc gtagcacgtc ctttgggtgt gtattctttc aaaggtgtct gcttcccaaa
     3181 tccatttgtg gtcacaacca gcaatg
//
LOCUS       DAOQFT010000280         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17759_length_3193_cov_7.503824, whole genome shotgun sequence.
ACCESSION   DAOQFT010000280 DAOQFT010000000
VERSION     DAOQFT010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17759_length_3193_cov_7.503824"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PLR93_07910"
     CDS             <1..165
                     /locus_tag="PLR93_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNH26928.1"
                     /translation="THNMQQAARASDYTAMMMIDDQRAGGVIEFDDTKNIFTRPKDKR
                     TEDYVTGRFG"
     gene            311..1000
                     /gene="phoU"
                     /locus_tag="PLR93_07915"
     CDS             311..1000
                     /gene="phoU"
                     /locus_tag="PLR93_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNH26929.1"
                     /translation="MIRKTFESDIQQVKDEVLALGSMVEHAIISSVEALKKRDIKAAE
                     KIIAEDKEINKKRFAIENQLMILIATQQPMAHDLRLLASTMEIISELERMGDYAKGIA
                     NINVRMADEKLLKPLIDIPRMAEKGASMLHRALTAFVHEDIETAKSLPIEDDEVDALY
                     NQIYRELMIFVIEDPKNIERANWLLWVAHNLERVADRVTNICERTVFIATGEFGEIKS
                     SDDEFWKNQNK"
     gene            complement(1099..1860)
                     /gene="pstB"
                     /locus_tag="PLR93_07920"
     CDS             complement(1099..1860)
                     /gene="pstB"
                     /locus_tag="PLR93_07920"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNH26930.1"
                     /translation="MNAPQPAFTVQNMDFYYNTSKKTLSNINLEIQPQKVTALIGPSG
                     CGKSTFLRCLNRMNDTIPGTKVDGKIFLNGEDIYGETMDVVTLRQRVGMVFQKPNPFP
                     QSIFDNVAFGPRVMGMDVKLSEVVEKSLRAAALWDEVKDDLKSDAMGLSLGQQQRLCI
                     ARVIAVQPEVILMDESTSALDPIATLRVEELIAELKKQYTIVIVTHNMQQAARVSDYT
                     GFFWLGEMVEFAQTNQMFTVPKQELTEAYITGRMG"
     gene            1977..2849
                     /locus_tag="PLR93_07925"
     CDS             1977..2849
                     /locus_tag="PLR93_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH26931.1"
                     /translation="MKRVIVFLLLLSILLISCGSSSTDTSSDSPAQYIENKGSDTIVN
                     LALAWAETYQAEFPDVRISVTGGGSGTGIAALINGTVDIANASRKIKDEELTEAQSKG
                     VDPLEHIIARDAIAVIVNPENPVNELTLQQIADIYSGKITNWREVGGEDRPIVKLSRE
                     TNSGTHVYFLETVLRLGDKENKTLFSTDTLLLPSSEGIIAEVRQNPNAIGYDGLGYVP
                     DDLKMIAIAKETGGTYVLPAIATVNDKTYPIARDLYMYTNGEPTGAVKEYLDWILSPE
                     AQEIVAKLGFVPVK"
     gene            2901..>3193
                     /locus_tag="PLR93_07930"
     CDS             2901..>3193
                     /locus_tag="PLR93_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNH26932.1"
                     /translation="MEKSLNWREFIITRAIKASGYSAIVFVLLIFFFLMREGLPALSE
                     VETSALFSTRWYPIENYFGILPLITGSLIVTLGAALIAVPFGIGTAIYISEIAP"
BASE COUNT          886 a          841 c          709 g          757 t
ORIGIN      
        1 actcacaaca tgcaacaagc cgcgcgcgcc agtgactata cagccatgat gatgatcgac
       61 gaccaacgtg ccggcggtgt gatcgaattt gacgatacca agaacatctt cacccgcccc
      121 aaagacaagc ggaccgagga ttacgtcacc ggaagatttg gataaaaagc cgcctctttc
      181 agacgttttg cctgttcgcc cgcccaaacc tttctctccc cttgagcgga cccttccgaa
      241 acggcaccgt cagatagaat cgataatcgc aaaagtagta aaatgaacac aattaaccta
      301 aagaggactt atgatccgta aaacttttga gagcgacatt caacaggtaa aagatgaagt
      361 gctggcactt ggctcaatgg tggaacatgc catcatcagc tcagtggaag cgttaaaaaa
      421 gcgcgatatc aaagccgctg aaaaaattat tgccgaagat aaagagatca acaaaaaacg
      481 tttcgccatc gaaaaccagt tgatgatcct cattgccacc caacaaccca tggcgcacga
      541 cctgcgtctg ctcgcctcca ccatggagat catctctgag ctggaacgca tgggcgatta
      601 cgccaaaggc attgccaaca tcaacgtccg catggcagat gagaaattgc tcaagccgct
      661 gatcgacatt ccacgtatgg cagagaaagg cgccagcatg ctgcaccgcg ccctaaccgc
      721 ctttgtacat gaagatatag agaccgccaa gtccctcccc atcgaagatg acgaagtgga
      781 cgcactctat aaccagatct atcgtgagtt gatgatcttc gtgatcgaag accccaagaa
      841 catcgaacgc gccaactggc tgttgtgggt tgcccacaac ctcgagcgcg ttgcagaccg
      901 tgttaccaac atctgcgagc gcaccgtctt catcgccacc ggtgaattcg gcgagatcaa
      961 atccagtgac gatgagttct ggaagaatca gaataagtaa tacgttacaa gaaataaaaa
     1021 gcgagtggat gagaaaactc atccactcgc tttgtttaat aactacaagg ctattcgact
     1081 aacaaactac cagactaatt agcccattct tccggtaata tacgcttctg tcaattcttg
     1141 ctttggcacc gtaaacatct gattggtctg cgcaaactca accatctctc ctaaccagaa
     1201 aaagccggtg taatcagaaa cacgcgcagc ttgttgcatg ttatgggtca cgatcacgat
     1261 cgtgtactgc ttctttaatt ctgcgatcag ttcttccacg cgcagtgtgg caattgggtc
     1321 caatgccgaa gtcgattcat ccatcagaat cacttccggt tgcacagcaa tgacgcgtgc
     1381 aatgcacagg cgttgttgct gccccaatga caagcccatc gcgtctgatt tcagatcatc
     1441 ttttacttcg tcccaaagtg ccgccgcccg cagagatttt tccacaactt cgctgagctt
     1501 cacatccatc cccatcaccc gcggaccgaa cgccacgtta tcgaaaatgg attgcggaaa
     1561 tgggttcggc ttctggaaga ccatccccac ccgctgacgg agtgtcacta catccatcgt
     1621 ttcgccataa atatcttcac cattgagaaa gatcttccca tccactttgg tgccgggaat
     1681 ggtgtcattc atgcggttaa gacaacgcaa gaacgtagat tttccacaac ccgatggacc
     1741 gatcaacgcc gtcacctttt ggggctgaat ctccaggttg atgttcgaga gggtcttttt
     1801 tgatgtgtta tagtaaaagt ccatgttctg gacggtaaag gcaggctggg gtgcgttcat
     1861 gatttgattg tatcaaaaag gaaaatcaaa gcggtaaaaa aaattcataa agtggcaaaa
     1921 agcgtcaggg tcagaatcaa gcctccgtca agtttgaggc ggtataatcc cagccgatga
     1981 aacgtgtaat cgtcttctta cttcttcttt ctattctatt aatctcttgt ggttcttcat
     2041 ccactgacac aagctctgac tcccccgcgc aatacatcga aaacaaaggc tccgatacca
     2101 tcgtcaatct ggcgttggca tgggcagaaa cctatcaagc cgaattcccc gatgtgcgca
     2161 tctcggtgac aggcggtggt tcaggcacag gcattgccgc gctcatcaac ggaaccgtgg
     2221 atattgccaa cgcttcacgc aaaatcaaag atgaagagtt gaccgaagcc caatccaaag
     2281 gggtggaccc gcttgagcac atcatcgcac gcgatgcgat tgccgtgatc gtgaacccag
     2341 aaaaccccgt caacgaattg acgttgcagc agatcgcaga tatttacagc ggcaagatca
     2401 ccaactggcg tgaagttggc ggcgaagatc gacctatcgt aaaactttca cgcgaaacca
     2461 actcaggcac ccatgtttac tttttggaaa ccgtcttaag attgggagac aaagaaaaca
     2521 aaacgttgtt ctcaacagac accttgctac ttccgtcttc agaaggcatc attgccgaag
     2581 taaggcaaaa cccgaacgcg attgggtacg atggcttggg ctatgttcca gatgacctca
     2641 agatgatcgc aattgcaaaa gaaacaggcg gcacttatgt cttacctgcc attgctaccg
     2701 tgaacgacaa aacctatccc attgcgcgtg acctttatat gtataccaat ggtgaaccca
     2761 ctggcgctgt gaaagaatat ctcgattgga tcctctcccc tgaagcgcaa gagattgtgg
     2821 caaaacttgg gtttgtgcct gtgaaataaa tcatttcgtc attgcgatga tcttcaccct
     2881 cgcaatgacg atggagcact atggaaaaat ctttaaactg gcgagaattt atcatcaccc
     2941 gagccatcaa agcgagcgga tattccgcca tcgtatttgt attgcttatc tttttctttc
     3001 tcatgcgtga aggcttgccc gccttgagcg aagtggaaac cagcgcactc tttagcacac
     3061 gttggtatcc cattgaaaat tatttcggta ttctgccact catcaccggt tctctcatcg
     3121 tcacccttgg cgcagcgctt atcgccgtgc ctttcggaat cggaactgcc atctacattt
     3181 ctgaaattgc gcc
//
LOCUS       DAOQFT010000281         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17764_length_3192_cov_8.299331, whole genome shotgun sequence.
ACCESSION   DAOQFT010000281 DAOQFT010000000
VERSION     DAOQFT010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17764_length_3192_cov_8.299331"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLR93_07935"
     CDS             complement(<1..614)
                     /locus_tag="PLR93_07935"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH26933.1"
                     /translation="MKLEVITRIPKEQKYKTPLLFVHGMWHGAWCWDEFFLPFFADAG
                     YHVTALSLRGHAGSEGKIQGSSIMDYVRDVEEVARTLPTPPVLIGHSMGGFIVQKFLE
                     RNTAPVGVLLAPAPPYGVWNGTWQVFKHSPLTLMKILTRFRLHPVVETPESTRWAFFS
                     ADMPQDQLLKYHAKMNDESFRMYLDLLGLNLVRTKKVKTPLLVLG"
     gene            complement(687..758)
                     /locus_tag="PLR93_07940"
     tRNA            complement(687..758)
                     /locus_tag="PLR93_07940"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Lys,seq:ttt)
     gene            856..1179
                     /gene="trxA"
                     /locus_tag="PLR93_07945"
     CDS             856..1179
                     /gene="trxA"
                     /locus_tag="PLR93_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNH26934.1"
                     /translation="MSSVKYVTEQDFQDEVISSQTPVLVDFTADWCQPCKMIAPIVEQ
                     LAGQWDGKVKVVKLDADQNPNIMMQYGVMGIPTLMLFKGGDVKERITGYLPKDKIEAK
                     VTPHV"
     gene            1233..>3192
                     /locus_tag="PLR93_07950"
     CDS             1233..>3192
                     /locus_tag="PLR93_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH26935.1"
                     /translation="MSEQQLIQAGISALQSNDKQKAFSIFAQVVQQYPQSERGWFLLG
                     MSTDDTQRRKYCLQKVLSINPNHADAKKQLTLLTPPQPAPPTPTFTSFNRSDFQQQHV
                     PAFVDNEPDATSEFERFSEPLNQPEVQQEPQHQPTPKQTKQTRRKKNNNVILIASISG
                     LCILAFTAMAGFYLLMNSRTTPAVQAANPTQPISATNTTLAMAPVATDTPIPPTPLPT
                     GLPTQPYTPRFEEAACQFDVPPGVNVSCGYAIVPENRTINSGRTIRLAVAIFHSRSSN
                     PKPDPVIFLQGGPGGEAVSLSANAYPILVDPFLSDRDYIAYDQRGTGLSEPALICDEL
                     ERANRQDVYGTIDSGSRELVYQNAFLSCAGLLQTQGVDLSAYNTVENAADLRDIVTLL
                     GYEKVNLYGASYGTRLALVAMRNHPEVIRSAILDSVMPVETNWVTEFPNIANYTLTNL
                     FNACKADPDCNKAYPDIETVFWELASKLDEKPITLSTSAYPMGTVTETIDGSYLLSII
                     MGLSKTTRFIDTAPQTIYRVRDGDYSTLIAAQYSLPYGFEGINTGLYISVLCREHVLD
                     TSPEKLLEAATQLRVKDFVWSPFYGHLDKMFSACKTWGSTGPVLGEKDKVVSDIPSLV
                     ITGTFDGATPPQQGKQVADSLPNSYYFEF"
BASE COUNT          873 a          839 c          703 g          777 t
ORIGIN      
        1 ccaaggacaa gcaacggtgt tttcactttt ttcgtgcgga ccaagttcaa ccccagcaag
       61 tctaaataca tacgaaacga ctcgtcgttc attttggcgt gatactttag aagttggtct
      121 tgtggcatat ctgccgagaa aaatgcccaa cgtgtgcttt caggcgtctc aacgacagga
      181 tgtaaacgaa atcgggtgag gattttcatc aacgtaagcg gactatgttt gaatacctgc
      241 cacgtcccat tccaaactcc gtacggcgga gccggagcca gcaaaacacc caccggggct
      301 gtatttcgtt cgagaaattt ctggacgatg aacccgccca tggagtgacc gatcagcacg
      361 ggcggagttg gaagcgtccg cgctacctct tccacatctc gaacataatc catgatacta
      421 ctgccttgaa tcttcccctc actgccagca tgtccacgca ggctgagcgc cgtgacgtga
      481 taccccgcat cggcaaaaaa gggcaaaaag aactcgtccc aacaccaggc tccatgccac
      541 atgccatgga cgaaaagcag cggggtctta tatttttgtt ctttaggaat tcgagtgatc
      601 acttcaagtt tcatggcaaa ctctcctctc ccttttgagt ttactgtata aaacaaaaat
      661 ccctcacata ggtaagggat tttctgtgag cgcggagggg atcgaaccct caacaaacgg
      721 cttaaaaggc cgctgctctg ccattgagct acgcgcccat ctttaaagcg gattgcattc
      781 tatcacggca gcatactcac gtcaacggaa gtaattaaaa tagagaacat ctcatccaag
      841 acctcggagg caaccatgag ttcagtcaaa tatgtaacag aacaagactt tcaagacgaa
      901 gtcattagct cgcaaacccc tgtattggta gatttcaccg cagattggtg tcagccgtgt
      961 aagatgatcg cacccattgt cgaacagctc gccggtcaat gggatggcaa ggtcaaggtc
     1021 gtcaaacttg atgcggatca aaaccccaac atcatgatgc agtacggcgt gatgggcatc
     1081 cccaccttga tgcttttcaa aggcggagat gtcaaagaac gcattacggg ctatctaccc
     1141 aaagacaaga tcgaagccaa agtcaccccc cacgtgtaag agtataatgg agccaacata
     1201 gtcggctcca ttttctttta tatagggaaa atatgtcaga acaacaactt attcaggcgg
     1261 gaatttctgc gctacaaagc aatgataaac aaaaggcatt ttcgatcttt gcccaagttg
     1321 tacaacagta ccctcaatct gagcgtggct ggtttctgct tggcatgagc acagatgata
     1381 ctcaaagacg gaagtattgc ctgcaaaaag ttctaagtat caaccccaat catgcggatg
     1441 ccaaaaagca gcttacgctt ctcaccccgc cccagcctgc tccaccaacc ccaacattca
     1501 cttctttcaa ccgctcagac ttccaacagc aacacgtccc cgcttttgtt gacaatgagc
     1561 cagacgccac ttcggaattt gagcgattct cagaaccgct gaatcagccc gaggtccagc
     1621 aagagccgca gcaccaacct actccaaaac agaccaagca aacccgtcgc aagaagaata
     1681 acaacgtcat cctgatcgca tccattagcg gactttgcat tctggccttt accgccatgg
     1741 ctggctttta tttgttgatg aacagccgta cgacaccggc agttcaagca gcaaacccga
     1801 cacagcccat ttcagccacg aacacaacct tggcaatggc tcctgttgcg acggatacgc
     1861 ccatcccgcc tacaccactt cccaccggct taccaacaca gccatacaca cctcgctttg
     1921 aagaagcagc ttgtcaattc gatgtgccgc ccggggtgaa cgtttcttgt ggttatgcca
     1981 ttgtgcctga aaaccgcact atcaacagcg ggcggaccat ccgcttggcg gttgctatat
     2041 ttcatagccg aagctccaat cccaaaccag acccggttat ctttttacaa ggtggtccgg
     2101 gtggcgaggc ggtttcgctc agcgcaaatg cctacccaat cttagtggat ccatttttga
     2161 gtgaccgaga ttacatcgct tatgaccaac gtggcaccgg gctttcggaa ccagccttga
     2221 tctgcgatga gcttgaacga gctaatcgac aagatgttta cggtactatc gatagcggtt
     2281 ccagagagtt ggtgtatcag aacgccttcc tctcttgtgc cgggctttta caaactcaag
     2341 gcgttgacct cagcgcgtat aacacggttg aaaatgcagc cgacttaaga gacattgtca
     2401 cattactggg atacgaaaag gtcaatttat atggcgcttc atatgggaca cgtctcgcat
     2461 tggttgccat gcgcaatcat cccgaggtta ttcgcagcgc tattttagat tcggtcatgc
     2521 cggtagaaac caattgggtg acagaatttc ccaacatagc caattacact ctaacgaatt
     2581 tatttaatgc ctgcaaagca gaccctgatt gcaataaagc ctacccagat attgaaaccg
     2641 tattttggga gcttgccagt aaattagacg aaaaaccgat aactttatct acatctgcgt
     2701 acccgatggg aacggttacg gagaccatcg atggctccta tttattatcg atcatcatgg
     2761 ggttatcaaa aactacccgt ttcatagata ctgctccaca aaccatctat cgcgtacgcg
     2821 atggggatta ttcaactttg atcgccgctc aatattcatt gccgtatggg tttgaaggca
     2881 ttaatacggg tctgtatatc tcagttctgt gtcgcgaaca tgttttggat acatctcccg
     2941 aaaaattgct ggaagctgct actcagttaa gagtcaaaga ttttgtttgg agcccttttt
     3001 atgggcattt ggataagatg ttctctgcct gcaagacttg gggctcaacg ggaccggtac
     3061 tcggcgaaaa agataaagtc gtcagcgata ttcccagttt ggtcatcacc ggaaccttcg
     3121 atggtgctac tccgccacaa caaggcaaac aggtggcaga ctcattaccc aatagctatt
     3181 attttgaatt cc
//
LOCUS       DAOQFT010000282         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17819_length_3187_cov_7.176564, whole genome shotgun sequence.
ACCESSION   DAOQFT010000282 DAOQFT010000000
VERSION     DAOQFT010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17819_length_3187_cov_7.176564"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLR93_07955"
     CDS             <1..663
                     /locus_tag="PLR93_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HNH26936.1"
                     /translation="AIVYVQQGRRNVPVMYPGRRVGNRMSMPVKGTMPLMVNLSGMIP
                     LIFASAILQFPAIIASYFTQSENQAVANFFVGIQNFFGATGTSNWGYWTIYFFMVVVF
                     TFFYTTVLFDQQNYGENLKKVGASVPGVLTGAPTQKYLSTVQSRITFVGALFLGIVAI
                     MPFLLGLLLAAFNISASNSTGIFLITSSGLLIVVGVVRDTFQNIDAELKLHGYQDSLL
                     VR"
     gene            676..1326
                     /locus_tag="PLR93_07960"
     CDS             676..1326
                     /locus_tag="PLR93_07960"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNH26937.1"
                     /translation="MPTYIVLLGPPGVGKGTQARILSEETGLVHVSSGDLFRENLKNQ
                     TELGKLAKSFMDKGELVPDDVTINMIKDRISRPDCKAGAVLDGFPRTPAQADALEVML
                     KSFEGAVNAVPYITAAEHILVERASGRWTCRANGHIYHEKFNPPKQARVCDIDGSEVY
                     QRDDDKVETVTKRIRVYLEQTMPLVEYYRKAGKLIEIDGTQAVEEVTKVLLSALKK"
     gene            1378..2148
                     /gene="map"
                     /locus_tag="PLR93_07965"
     CDS             1378..2148
                     /gene="map"
                     /locus_tag="PLR93_07965"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNH26938.1"
                     /translation="MFHERQINLKTPAELKIMREAGRINASVHAKLRELLQPGVTTAD
                     LNAAAEEVLKSHGCVSPFKGYGHPPFPASITVSINDEMVHGIPNSKRKLKEGDVVSLD
                     CGTVYQGYVADSAFSAGVGQVSQEAQTLLEVTEAALQAGIAKMRKGNRTGDVSAAIQK
                     YVESHGLHVTREYTGHGVGKSMHEGPQVPNYGMAGTGLVLKPGMTIALEPMVLVGTYE
                     TRVLPDKWTVVSADGSLTAHFEHTVAVTEGEPQILTVL"
     gene            2248..2361
                     /gene="rpmJ"
                     /locus_tag="PLR93_07970"
     CDS             2248..2361
                     /gene="rpmJ"
                     /locus_tag="PLR93_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNH26939.1"
                     /translation="MKVSPSIRKRCAKCRIVRRKGIVYIICENPKHKQRQG"
     gene            2369..2755
                     /gene="rpsM"
                     /locus_tag="PLR93_07975"
     CDS             2369..2755
                     /gene="rpsM"
                     /locus_tag="PLR93_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNH26940.1"
                     /translation="MARIEGVDLPRNKRTEVGLTYLFGIGPTRAKKILADTKVNPDTR
                     IKDLTEAEVSAIREYIAKHYKVEGDLRREVQMNIKRLIEIGSYRGLRHRRNLPVNGQR
                     TKTNARTRKGTKKTVAGRGRRRGAKK"
     gene            2780..3184
                     /gene="rpsK"
                     /locus_tag="PLR93_07980"
     CDS             2780..3184
                     /gene="rpsK"
                     /locus_tag="PLR93_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNH26941.1"
                     /translation="MAQSVRSTRRAGGAKKGKRSLSRGQVHIFASFNNTIVTVTDTEG
                     NTISWGSAGSAGFKGSRKSTPFAARLAAEQAIKSAQQQGLQEVELYVKGPGPGRENAI
                     RAVQAMGLKVLLIADITPVPHNGCRARKSRRV"
BASE COUNT          823 a          790 c          819 g          755 t
ORIGIN      
        1 gccattgtgt acgtccaaca aggacgccgc aatgtgcccg tcatgtaccc tggtcgccgc
       61 gttggcaacc gcatgtccat gccggttaag ggcaccatgc cgttgatggt taacctttct
      121 ggcatgatcc ccttgatctt tgccagtgcc atccttcaat tccccgcgat tatcgctagc
      181 tactttacac agagcgaaaa tcaagcagtt gctaatttct ttgttggaat tcaaaacttc
      241 tttggcgcca ctggcaccag caattggggt tattggacga tctacttctt catggttgtg
      301 gtatttactt tcttctatac aaccgtgttg tttgaccaac aaaattatgg cgaaaacctg
      361 aaaaaggttg gagctagcgt ccccggtgtg ttaacgggtg cgccaactca aaaatacttg
      421 agcacggttc agagccgcat caccttcgtg ggcgctctct tccttggtat tgtcgctatt
      481 atgcctttct tgcttgggtt gttgctagct gcctttaata tctcagcttc caacagcaca
      541 ggtatcttcc ttattacttc ttcgggcttg ctcattgtgg tgggtgtcgt tcgcgatacc
      601 ttccagaaca tcgatgccga gctgaaattg cacggctatc aggactcttt gctcgtccgc
      661 tagaggtaaa tacaaatgcc aacctatatc gtcttgctcg gacctcctgg tgttggtaag
      721 ggaacgcagg cacgaattct ttccgaagag accgggttag tgcacgtttc atccggtgac
      781 ctctttcgcg agaaccttaa gaaccaaacg gaacttggaa agctcgccaa atccttcatg
      841 gacaaaggcg agcttgttcc agatgatgta accatcaaca tgataaagga ccgcatctct
      901 cgtccggact gtaaggctgg tgctgtgctc gacggctttc cccgcacccc ggctcaagca
      961 gatgcgctcg aggtgatgct caaaagcttt gaaggagctg tcaatgcagt tccatatatc
     1021 acggctgccg aacacatcct cgtagaacgt gccagcggac gttggacctg ccgcgcaaat
     1081 gggcacattt atcatgaaaa gttcaacccg cctaagcagg ctcgagtctg cgatatcgat
     1141 ggttccgaag tctaccaacg tgatgatgat aaagtagaaa cggttacaaa gcgtatccgt
     1201 gtctatcttg aacagaccat gccgttggtt gaatactatc gtaaggctgg caaattgatc
     1261 gaaatcgacg gcactcaagc ggttgaagaa gtaacaaaag tattactgag tgctctcaaa
     1321 aaataacgaa agaccagagt gtcgactctg tacaaataaa gtaaggtttt gcaaaaaatg
     1381 tttcatgaac gccaaatcaa tctcaagact cctgctgaac tcaaaatcat gcgagaagca
     1441 gggcgcatca atgccagcgt tcatgcgaaa ctaagagaac ttttacaacc aggtgtgaca
     1501 acagcggacc tcaacgcggc tgctgaggaa gtgctgaagt cacacggatg tgtatcgccg
     1561 ttcaaaggct atggacaccc gccgtttcct gcatccatca cagtcagtat taatgacgaa
     1621 atggtgcatg gaattcccaa ctcgaagcgc aagctgaaag aaggggacgt cgtttcactc
     1681 gattgcggta cggtgtatca aggttatgta gcagactccg cgttttcggc tggggtgggg
     1741 caggtctcac aagaggctca gaccctgctc gaagtgacgg aagcggcatt gcaagcgggc
     1801 atcgcaaaga tgcgcaaagg caatcgtaca ggagatgttt cggcggcgat acaaaagtat
     1861 gtagaaagcc atgggttgca tgttactcgc gaatacaccg gacacggtgt cgggaagagc
     1921 atgcatgagg gtcctcaagt gccaaattac ggcatggcag gaaccggtct cgtcctcaaa
     1981 ccggggatga cgattgccct cgagcctatg gttcttgttg ggacgtatga aactcgcgtc
     2041 ctgccggata agtggacggt agtttccgca gatgggtcgt tgacagcgca ctttgaacat
     2101 acggtggctg ttaccgaagg agaacctcaa atcctgactg tcttatgacc ctgcgggtag
     2161 ccgtaaaaac atggacgaaa ttgagaacga ccagtataat taagcgtttg gctttgccag
     2221 agaagttgca aagcaaggag aaatttcatg aaagtatcgc cctccattcg caagcgttgt
     2281 gccaagtgcc gtatcgttcg gcgcaaaggt attgtttaca tcatttgcga aaatcctaaa
     2341 cacaaacaga gacaagggta gttgaaccat ggcaagaatt gaaggtgttg atcttccccg
     2401 caacaagcgg acagaagtgg gtctgaccta tctttttggt attggaccta cccgcgccaa
     2461 aaagattttg gcagatacca aagtcaatcc tgacacgcgc atcaaggacc tgacggaagc
     2521 tgaagtttcc gccatccgcg aatacatcgc caagcactat aaggtggaag gcgatttgcg
     2581 ccgtgaagtt cagatgaaca tcaagcgttt gatcgaaatc ggttcgtatc gcggcttacg
     2641 ccatcgccgc aatttgcccg tgaatggtca gcgcaccaaa accaatgcgc gtactcgcaa
     2701 aggcaccaag aaaacggttg ccggacgcgg tcgtcgccgt ggcgctaaga agtaatcctg
     2761 tccgaaaggt taagaaatta tggctcagag tgtacgttct acgcgccgcg caggcggcgc
     2821 gaaaaaaggg aagcgctccc tgtctcgcgg acaggttcat atcttcgctt ccttcaataa
     2881 cacgatcgtt accgttaccg acactgaagg caacaccatt tcctggggat cggctggttc
     2941 cgctggcttc aaaggctccc gcaaaagcac tccttttgcc gcacgcctcg ccgctgaaca
     3001 ggcgatcaaa tctgctcaac agcagggttt gcaggaagtg gaattgtatg ttaaaggtcc
     3061 cggacccgga cgcgaaaatg caattcgcgc cgtccaggcg atgggcttga aggtgttgtt
     3121 gatcgcggat attaccccgg tgccacacaa tggctgtcgc gcacgcaaga gtcgtcgagt
     3181 ttaattt
//
LOCUS       DAOQFT010000283         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_17947_length_3173_cov_6.291854, whole genome shotgun sequence.
ACCESSION   DAOQFT010000283 DAOQFT010000000
VERSION     DAOQFT010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17947_length_3173_cov_6.291854"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="PLR93_07985"
     CDS             <1..1709
                     /locus_tag="PLR93_07985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH26942.1"
                     /translation="VPTAESMLDIFSSLLTADTDLENLTAALVETQDRLVALYELTQA
                     TRRTLDMPKLLDLLIQQSKHLMDVDGGFVVLKEKGKQTIVHQVSENPLYPSHAEAAAS
                     LYLRDPNRHVFKDSETLPANLKNVMMVTLPVRDEISAAVGFFNKTGLFTTPDIKLAKA
                     IAGHIGAQLENAMLHKEAVERARLETEMDIARQVQTAILPQSIPQVSGVDIFATSIPA
                     FEVGGDFFDVIDRSAESLIFSLGDVTGKGMPAALLMSMAHTIIKAASRNMPFDHPHQV
                     LNRLNHDMESDFSNVGMFTTVMLGMVDCANCKLHFSNAGQSPIYYMPVNGDAVLLEAQ
                     DIPVGILDTVEYTTQSLSLAHGDLFVAASDGFPESRNPVGEMYGYERMKNILSASRQL
                     PSKQIAEALLNDVNTFCETHPQDDDRTIIVLKFEKQNEKQIMFNDSLTVRANYEDIRQ
                     PCERLRTNLSQSGVSEEIAGLCELALQELLTNLVDHAYEGNLNGQITVRMASKNGQIF
                     LQTEDTGLPARIKMEDVHMPNPEDLAEGGYGMAIIRSLMDDIRYESMQGKNIWTLIKN
                     LK"
     gene            1734..2078
                     /locus_tag="PLR93_07990"
     CDS             1734..2078
                     /locus_tag="PLR93_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH26943.1"
                     /translation="MSLEINSTEQVIKIAAITLKGRLDAFEAPALRKTCDAYIADEFS
                     HFVFDLTEVAMLDSAGLAVLVSVLKRARLKNGDVRLVWPREEAASRILKLTKFDQVFV
                     SIEKGEIVPRGF"
     gene            2080..2454
                     /locus_tag="PLR93_07995"
     CDS             2080..2454
                     /locus_tag="PLR93_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH26944.1"
                     /translation="MSKAKILVVDDEPNNQRILTYTLNKAGYESAAIADGENTLVWLA
                     SNTPDLAILDVSMPGMDGITLLTHIRSMPQYSRLPVIILTGSGDDDERLRAEGLGIQA
                     FLTKPASSKTVLEAVANLLPHE"
     gene            2447..>3173
                     /locus_tag="PLR93_08000"
     CDS             2447..>3173
                     /locus_tag="PLR93_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNH26945.1"
                     /translation="MNKLKELLRSKLASASALLMLSMTVVNAGNYIYNLIMGRWLGPS
                     LFADLSLIVTLLLVVTFLTAPIQMTAARYSAIHTADGDDKTLASLRRFIWLVALGVGL
                     TLTAFFAIFATTLKVFFHTQSALPFVIFGAALPFGMMQAVERGMLQGRTSFKVLAISY
                     QVEMWSRLLVSILLVALGLSVNGAVIGISLSFVFTWLVARSAVVGLNSQENISKEMRT
                     ELLAFAAPVLVSQLGQILINNSDV"
BASE COUNT          807 a          733 c          755 g          878 t
ORIGIN      
        1 gggtgccaac ggcggaatcc atgctggata ttttttccag cctgcttaca gcagatactg
       61 atctggaaaa ccttactgcg gcactagtgg aaactcaaga ccgacttgtg gcgttgtatg
      121 agttaacgca agccacacgc cgcactttgg atatgcccaa gttacttgat ctgcttatcc
      181 aacaatccaa gcatttgatg gatgtggatg gcggctttgt agtgttgaaa gaaaagggca
      241 agcaaaccat tgttcatcaa gtttcagaaa acccattata cccgtcacat gccgaggcgg
      301 cagccagttt gtacttacgg gaccccaatc gccacgtgtt taaggactct gaaacattac
      361 cggcaaattt gaagaatgta atgatggtca ctttgccagt gcgcgacgaa atttctgcgg
      421 cagttggttt tttcaataaa acagggctct tcactacacc cgatatcaag ttggcaaaag
      481 ccattgctgg gcatattggt gcgcagttgg aaaatgccat gcttcataaa gaagccgtgg
      541 aacgtgcccg gcttgaaacc gaaatggaca ttgctcgtca ggttcagacg gcaatccttc
      601 cgcagagtat tccgcaagtg agtggtgtag atatatttgc cacatccata ccggcatttg
      661 aagtaggcgg cgattttttt gatgtgattg accgttccgc agaatcgctc attttttctt
      721 tgggcgatgt tacagggaaa ggtatgcccg ccgctttgtt gatgagcatg gcgcatacga
      781 ttatcaaagc cgcatcgcgg aatatgccgt ttgaccatcc gcatcaggtc ttaaaccgtc
      841 tcaatcatga tatggaatct gatttttcaa atgtgggtat gtttactacg gtgatgctag
      901 gcatggtgga ctgtgcaaat tgcaagctgc attttagtaa tgccgggcaa agccccattt
      961 actatatgcc cgttaatggc gatgcagttc tgttagaagc acaagatatt cccgtcggta
     1021 ttttggatac ggtggagtac accacccaaa gcctaagctt ggcacacggt gacctttttg
     1081 ttgccgcatc agatggcttt cccgaatctc gaaacccggt aggggagatg tatggttacg
     1141 agcgtatgaa gaatatctta tccgcctcgc gccaattgcc ttccaaacaa attgccgaag
     1201 ccctgttaaa cgatgtgaat actttttgcg aaactcatcc acaagatgat gaccgcacca
     1261 ttattgttct taaatttgaa aaacaaaacg agaagcagat catgttcaat gacagtctaa
     1321 ctgttcgtgc caattatgaa gatattcgcc agccatgtga gcgcttgcgt acaaatttaa
     1381 gtcagagcgg ggtttcagaa gagattgctg gcttgtgcga acttgccttg caagaactac
     1441 ttacgaacct ggtcgaccac gcctatgaag ggaatttgaa cgggcagatc acagtgcgga
     1501 tggcaagcaa gaatggacag attttcctgc aaacggaaga taccggcttg cctgctcgta
     1561 taaaaatgga agatgttcat atgccaaacc ccgaagacct tgccgaaggc ggttatggca
     1621 tggcgatcat ccgttcgttg atggatgata ttcgctatga gtctatgcaa ggcaagaata
     1681 tctggacgct tatcaagaat ttgaaataat ggaatcaaga gaaagaggta actatgtcac
     1741 tcgaaattaa ttctacagaa caagtcataa aaattgctgc catcacacta aaaggcaggc
     1801 tcgatgcctt tgaagccccc gcgctacgaa aaacttgtga cgcatacatt gccgatgaat
     1861 tcagccattt tgtttttgac ctgaccgagg tagccatgct ggactcggct gggttggcag
     1921 tccttgtcag tgttctcaaa cgggcccgcc tcaagaatgg cgatgtgcgt cttgtctggc
     1981 cccgcgaaga agcggcatcc cgcatactca aactcaccaa gttcgatcag gtttttgtct
     2041 ctatcgaaaa aggcgagatc gtaccaagag gtttttaaca tgagcaaagc taaaattctc
     2101 gtagtggatg atgaacctaa caatcagcgc atccttactt acacccttaa taaagccggc
     2161 tatgaaagtg cagccattgc tgatggcgaa aacacgttgg tttggcttgc atctaatact
     2221 cccgatcttg ccattctgga tgtatccatg ccaggcatgg atggaattac tttgctaacg
     2281 cacatccgct ctatgccgca gtattctcgt ttgcccgtca ttatcctcac aggtagtggc
     2341 gatgatgatg aacgtctgcg tgccgaaggg ctgggcattc aagcttttct taccaaacca
     2401 gccagttcta aaacggtctt ggaagcagta gcaaatctac tccctcatga ataagctcaa
     2461 agaactactt cgctccaaac tggcatctgc cagcgcgctg cttatgctta gcatgaccgt
     2521 cgtcaatgcg ggcaattaca tctataacct tattatgggc aggtggctgg gtccgtcact
     2581 gtttgcagac ctttcactca ttgttacttt gctgctagtt gtgacctttc taaccgcccc
     2641 tattcagatg accgctgcgc gttattctgc cattcatacc gctgatggag atgataaaac
     2701 cctcgcctct cttcgacgtt ttatctggct cgtcgccttg ggggtaggtc tcacgctgac
     2761 agctttcttt gccatttttg caactacatt aaaggttttc tttcatacgc aatctgctct
     2821 tccttttgtt atttttggcg cagcacttcc ttttggcatg atgcaagctg ttgaacgtgg
     2881 aatgcttcag gggcgcacca gtttcaaggt tttagcaatc agttatcaag tcgagatgtg
     2941 gagccgtttg ttggtcagta tccttctggt tgccttgggg ctttcggtca atggtgctgt
     3001 gatcggcatt agtttgtctt tcgtattcac atggctggtg gcgcgctctg cagtagtcgg
     3061 gctgaattca caagaaaata tttctaaaga aatgcgtacc gaattgctgg cgtttgccgc
     3121 acccgttctg gtttctcagt tagggcaaat tcttatcaac aatagcgatg tga
//
LOCUS       DAOQFT010000284         3163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_18033_length_3163_cov_6.124196, whole genome shotgun sequence.
ACCESSION   DAOQFT010000284 DAOQFT010000000
VERSION     DAOQFT010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18033_length_3163_cov_6.124196"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLR93_08005"
     CDS             <1..809
                     /locus_tag="PLR93_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH26946.1"
                     /translation="PGVSDMKASIAISLGAIEEAYKNGAKRAITLLCTSDEEIGSVTS
                     RELIQNMAKEAELVFVMEPALLDGSLKTWRKGGGGFRVKVHGRAAHAGGDHEKGRNAI
                     EEMAHQVLAIQKMTDYSKKTTLNVGVIHGGTVSNVVPDYAEIDIDVRILQPEEWGRVE
                     GMMMALKPVLDGTTVEVTGSLNRGPMPFDELMQATFGKAKSIAAEIGIDLKAGGTGGV
                     SDANLVAPLGIPVLDGLGAIGEGYHSEREYIFAGSVAQRVKLISALLVNW"
     gene            954..1463
                     /locus_tag="PLR93_08010"
     CDS             954..1463
                     /locus_tag="PLR93_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26947.1"
                     /translation="MNDLFTCADELSLAVKVAADSPDIYLRIGEPDDVLTPVFQIDEE
                     ELLIVTHRESPVQNLSLEETQALFAGSSGLAVQVWVYPSELDVYEVFDQFVMQGRSVI
                     SAARVAVNPQEMSDVLNQESNAVGILPRHWKAGNVREVYSVGIFPVLAVTQEEPQGNV
                     AQLIACLQK"
     gene            1560..2210
                     /locus_tag="PLR93_08015"
     CDS             1560..2210
                     /locus_tag="PLR93_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26948.1"
                     /translation="MNQDFNEIQQRVRHSWFKDGVGEIAVGGLFVALSLYFAGHYFAG
                     TRFLPSASNAPLYIDLGLGVVLLLGIVLTRKIIAVFKTHITYPRTGYVEYYIESKNSL
                     LVRIAIFIAGTLFVLLLIWVGRWVGSFKWIPAFLGVFFGGVLLFLRTKTAGLKRFNYH
                     ASLSILLGLGLTFSSMPIPGLSVYYAVFGVWLLGWGIWILAKYLRENPLPEGDEDE"
     gene            2203..2514
                     /locus_tag="PLR93_08020"
     CDS             2203..2514
                     /locus_tag="PLR93_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNH26949.1"
                     /translation="MNDDLRSLTDLDRMIHEPARLLLVTILSGVASADFLFLQRQTGL
                     TKGNLSAHLSRLEEAGYVKIEKTFKGKMPFTVCKLTSAGQKALQRYRQQVQNFMDKTR
                     S"
     gene            2587..>3163
                     /locus_tag="PLR93_08025"
     CDS             2587..>3163
                     /locus_tag="PLR93_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH26950.1"
                     /translation="MNTPLIELKNVSKTYSTAAGEFPALRNINLNVYAGEFLGVIGKS
                     GAGKSTLLNMINGVDELSEGEVIVNSNNSRVSVHNLSEDEKALWRGKTMGVVYQSFQL
                     LPMLTLVENITLAMDLADNFKPRESRERAMELLRLVEIEEHADKLPSLISGGQQQRVA
                     IARALANDPDILVADEPTGSLDSVTADHIFDV"
BASE COUNT          798 a          642 c          813 g          910 t
ORIGIN      
        1 gacctggcgt ctctgatatg aaagccagta tcgctatttc gttgggcgca attgaagagg
       61 catataaaaa tggagcaaag cgggctatta ctttgctatg tacttcggat gaggagattg
      121 gaagtgttac ttctcgtgaa ttgatccaga acatggcgaa agaagcagaa ctggtttttg
      181 tgatggaacc tgctttgctg gatggctctc tcaaaacctg gcgcaagggt ggcggtggat
      241 ttagggtcaa ggttcatgga cgggctgcac atgcaggtgg agaccatgaa aagggacgga
      301 acgctatcga agaaatggca catcaagttc tcgccattca aaagatgacg gattactcca
      361 aaaagaccac attgaatgtg ggggtcatcc acggtgggac agtttcgaat gtcgtgccag
      421 attatgcgga gattgatatt gatgtccgca ttttgcaacc ggaagagtgg ggacgggtgg
      481 aaggaatgat gatggctttg aaaccagtcc tggatggcac aactgtggaa gtgacagggt
      541 cactcaaccg tggaccaatg ccattcgatg agttaatgca ggctaccttt ggaaaagcca
      601 agtccattgc tgctgagatc ggcattgacc tcaaagcggg tggtacaggc ggcgtctctg
      661 atgcgaattt ggtcgcgcca ttaggaattc cggtcttaga tggtcttggt gcgattggcg
      721 aaggctatca ttcagagcgc gaatacattt ttgcaggtag tgtggctcaa cgtgtaaagt
      781 tgatctcggc attgcttgtc aattggtaat cattttctat ggctgttcca tatagcaaga
      841 agattgtttt tgtttttctt tttctttcct tttttttgtt ttcctgtgca ccaaaaccat
      901 tatcttcaaa tcagattatc acagcgtatt ccacagcatc tgcgcagcca tggatgaatg
      961 acttatttac ctgtgcggat gaactgtctc ttgcggtgaa agttgccgca gactcgcccg
     1021 atatttattt gcgtatcggt gagccagacg atgtactaac ccctgtattt caaattgacg
     1081 aagaagaact actcattgtc acgcatcgtg aaagcccggt gcaaaacctt tctttagaag
     1141 aaactcaagc tttatttgct ggttcaagtg gattggcggt acaggtttgg gtgtatccat
     1201 cagagttgga tgtgtatgag gtgtttgacc agttcgtgat gcaaggacga agcgtgatat
     1261 ctgcagctcg ggtggcggtg aatccgcaag agatgtctga tgtgttaaat caagaatcga
     1321 atgcggtcgg aatcctgccg cgacattgga aagcggggaa tgtgcgtgag gtgtattctg
     1381 tggggatttt tcccgttctt gctgtgactc aagaagagcc gcaggggaat gttgctcaat
     1441 taatcgcttg tttgcagaaa taaaattctc atcttagttt tatttttcac ttgacgcctc
     1501 ctaatgggat ggtattatct aaaccggttt gtagtgcaaa ccggtttgtg tggagtgtta
     1561 tgaatcaaga ctttaacgaa attcaacagc gtgtgcggca ttcctggttt aaggatggtg
     1621 tgggcgaaat tgcagtgggc ggattatttg tagcactttc actatatttt gccgggcact
     1681 actttgctgg cacacgcttt ttgccttcgg cttccaatgc gccgttgtac attgaccttg
     1741 gtttgggagt tgttcttctt ttaggcatcg tgctaacacg caagatcatc gctgtgttca
     1801 agacgcatat tacgtatccg cgtacgggat atgtagaata ctacattgaa tctaagaact
     1861 ctttattggt acgaattgct atttttatcg caggtacatt atttgttctg ttattgattt
     1921 gggttggaag atgggtgggg tctttcaagt ggattcccgc ttttctgggc gttttctttg
     1981 gcggagtctt gttatttctg cgcacaaaga cagctggctt gaaacgtttt aactatcatg
     2041 caagtttgtc catcttatta ggacttggac taacatttag cagtatgcct atccctgggt
     2101 tgagtgtgta ctacgctgtt tttggcgttt ggctgttagg ttgggggatt tggatcttgg
     2161 caaagtattt gcgtgagaac ccactgccag agggagatga agatgaatga tgacctccgt
     2221 tcactaaccg atctagatcg tatgattcac gaaccggctc ggctcttatt ggtaaccatt
     2281 ctctccggcg ttgccagcgc cgattttctc tttctccaac gccagaccgg tttgaccaaa
     2341 ggaaacctgt ctgcccatct ttcgcgcttg gaagaagctg ggtatgtgaa gatagaaaag
     2401 acctttaaag gaaaaatgcc tttcacagtt tgtaagctga ccagtgctgg gcaaaaggcg
     2461 ttacagagat atcgccaaca ggtacaaaat ttcatggata agacccgttc gtgagcaatt
     2521 gaaaactctt tgcaaataaa ggacacaaaa tcgcatcaac aacagcggta aaataaaatt
     2581 gaacttatga atacaccatt gattgaattg aagaatgtat caaaaacata ttctacggcg
     2641 gcaggcgaat tccccgcttt gcggaatatc aacctaaacg tctatgcggg cgaattttta
     2701 ggggtaattg gcaaatcagg tgcgggaaaa tctaccttgt tgaacatgat caacggagta
     2761 gatgaactga gcgaaggcga agtgatcgtc aactcgaaca attctcgtgt ttctgtgcat
     2821 aacctgagtg aagatgaaaa agcactctgg cgcggtaaaa ctatgggcgt tgtgtaccag
     2881 tcctttcagc ttctacccat gctaaccctg gtcgagaata tcacccttgc tatggatctg
     2941 gcagataatt tcaaaccccg cgaatcgcgt gaacgcgcta tggaactttt gcgtttggta
     3001 gagatcgaag aacatgccga taaactccca tccctcatct cgggtggtca gcagcaacgt
     3061 gttgcaattg cgcgcgcatt ggcgaacgac cccgatattc ttgttgccga tgaacccacc
     3121 ggcagcctcg actctgtcac cgcagaccac atctttgatg tct
//
LOCUS       DAOQFT010000285         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_18042_length_3162_cov_6.688445, whole genome shotgun sequence.
ACCESSION   DAOQFT010000285 DAOQFT010000000
VERSION     DAOQFT010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18042_length_3162_cov_6.688445"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLR93_08030"
     CDS             complement(<1..583)
                     /locus_tag="PLR93_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26951.1"
                     /translation="MTDYLFRGALEKLDKDVYELTQLEAERQYRKLIMIPSESTAPMA
                     VREALMSAFQNIYAEGYPSEESRWMSEEEILDHPMRLADYRRNGDPRYYKGVEYADAV
                     EALARRRAAEAFAANGFTADQIYVNVQALSGGPANNAVYQALLNIGDTVMGLDLLHGG
                     HLSHGSPVNRSGKWFKAVHYSVDANEKLDYEAIR"
     gene            799..1281
                     /locus_tag="PLR93_08035"
     CDS             799..1281
                     /locus_tag="PLR93_08035"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNH26952.1"
                     /translation="MSRIPSLLRIFGWGVFFLVFFLEFAPPAKSYLVTHIGRTLAIIV
                     PFCFSPLVVVGIFGADFMNARLRDTAAKQGHEVMARVVRTSYIGSEGNEYHSYPIIRF
                     ELEVEEDGKVRRASTELIDYGKVDYRNYPPGTQVRAKYDPQTGSIAMLDSRGRVLEKL
                     "
     gene            1494..2393
                     /locus_tag="PLR93_08040"
     CDS             1494..2393
                     /locus_tag="PLR93_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26953.1"
                     /translation="MFKNKWVLSLALVAILAGLTTTGAFAKGNLPTDQETTAYGIFMP
                     PPPGDFQLSDEALAAAAEVLGITTDELSAALDAGTTLDELATTYSVDIKDVLDAIRAV
                     MPAPANGGFANGQPPANGGQPGQGGQPGQMPLNDEALAAAAEVLGITTDELSAALAEG
                     TTLDELATTYSVDIQDVFDAINTVQPMPQGGPDGQNGQLGQMPLNDEALAAVAEVLGI
                     TTDELSTALDAGTTLDELATTYGVDVQTIFDAINTVQPMPQGGPNGQNGQMPGGQNGQ
                     QPSQGQGGPNGTPPNGQAPGTRP"
     gene            2457..3095
                     /locus_tag="PLR93_08045"
     CDS             2457..3095
                     /locus_tag="PLR93_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26954.1"
                     /translation="MKKNLYSFIFLLLIGALLSACSLPSAAETQSAQPVNDPNAVIPQ
                     SVATASVVTTSSLPAGMPTVETDLSKILSVLQSGNFSYLANLASEKYDASAYTVPGSL
                     VFNAAFPSSQTIYLNYGWCAKDLATLQQNLQHISTYFSFNGQQIPMEYVNTISTESSD
                     GLQCSNTGLLLSGWAPGTYQFQVKASFDSQVNDGSADYPAGDYISEYTVTVQ"
BASE COUNT          801 a          822 c          768 g          771 t
ORIGIN      
        1 ggcgaatggc ttcgtaatcg agtttctcgt tcgcatccac cgaataatgc acagccttga
       61 accacttgcc gctgcggttg acgggcgagc cgtgtgagag atgcccgcca tgcagcaggt
      121 cgagtcccat cacggtatcg ccgatgttca gcaaggcttg atagaccgcg ttgttggcgg
      181 gcccacccga aagcgcctgc acgttgacgt agatctggtc ggcggtgaag ccgttggccg
      241 cgaaggcttc ggcggcgcgt cggcgggcga gggcttccac ggcgtcggca tattccacgc
      301 ctttgtaata gcgcgggtca ccgttgcggc gatagtcggc gaggcgcatc gggtgatcga
      361 gaatttcttc ttcgctcatc cagcgcgatt cttcgctcgg gtagccttcg gcgtaaatgt
      421 tctgaaaggc agacatcaac gcttcacgca ccgccatcgg cgccgtcgac tcactgggga
      481 tcataataag cttgcggtac tggcgctccg cttcaagctg ggtcaattca taaacatctt
      541 tatccagctt ttcaagcgcg ccacgaaata aataatcggt catgctgttc tcctgttggg
      601 ggaaaggtat cagagtatca aaattcaaat aaagccgcac ccgttcaagg tccatgagag
      661 atgcggcatt ttgtcagtca gatgaattgt aggacgttca agggcttggg tcaagtagaa
      721 aatcctgaga gcgtggtaca gaaggttgat gttcaaaaaa ctccagcagg gatacgattt
      781 ggcaccagga gattgtatat gagcagaatt ccatcgctat tgagaatttt tgggtggggc
      841 gtattttttc tggtcttttt tctggaattc gcaccaccgg caaaaagcta tttggtcaca
      901 cacatagggc ggacgttggc aatcattgtt ccattttgtt tttcgccttt ggttgtggtc
      961 ggcatctttg gtgcagactt tatgaatgca cgattgcggg ataccgctgc caagcaaggg
     1021 catgaagtga tggcgcgagt ggtacgcact tcatacattg gtagtgaagg caatgaatat
     1081 cactcctacc cgatcattcg ttttgaactt gaagtggagg aagatggaaa agtgaggcgg
     1141 gcaagcaccg aattaataga ttatggcaag gttgattatc ggaactatcc gccagggact
     1201 caagtaaggg caaagtacga cccgcaaacc ggcagcattg ccatgctcga ctcgcgcgga
     1261 agggttttgg aaaaactttg atgcaaaaaa ggttcctgct gatgaagcag gaaccttttt
     1321 ataagccaag tgagtaaaaa atccatcgtt tcatttatat agcctaaaca tcttcacaga
     1381 aacttctcag tatctgcata gaaaaatcac agcaaatcca cagtctcggc ttctatcatg
     1441 acggcgttgg tgagatcgcc aacaaatctt tagtgtcaag aaaaggagac tatatgttca
     1501 aaaataaatg ggtgctcagc cttgctttgg tagctattct cgcagggttg accacaaccg
     1561 gagcgtttgc gaaaggcaac ctccccactg atcaagagac aactgcatac ggcattttca
     1621 tgccgccccc tcccggtgat tttcagttaa gcgatgaagc gctggcggct gccgctgaag
     1681 tcctcggcat cactaccgat gaactttccg ccgctttgga tgcaggtacc actcttgatg
     1741 agttggcaac cacctatagt gtggatatta aggatgtact cgatgcgatc cgtgctgtga
     1801 tgcctgcacc cgccaacggt ggttttgcaa acggacagcc gcccgccaat ggtggtcaac
     1861 ccggtcaagg tgggcagcct ggtcagatgc ctttgaacga tgaagctctg gcagccgctg
     1921 ctgaagtcct tggcatcacc accgatgaac tttctgcagc tcttgcagaa ggcacaaccc
     1981 tcgatgaact tgccactact tatagtgttg atattcagga tgtgttcgat gccatcaata
     2041 cagttcaacc catgccgcaa ggtggacccg atgggcagaa cggtcagctt ggtcagatgc
     2101 ctttgaatga tgaagcattg gcagcagtgg cagaagtcct cggcatcacc actgacgaac
     2161 tctccaccgc actcgatgct ggcaccacct tagacgaatt agccaccacc tacggcgttg
     2221 atgttcaaac cattttcgat gctatcaaca ccgtccagcc catgccacaa ggcggtccga
     2281 atggacaaaa cggtcagatg cccggcggtc agaacggtca gcagcccagt caaggtcaag
     2341 gtggtccgaa cggaacacct cccaatggtc aggctcctgg cacacgtccg taactcttta
     2401 taaaaatgtc agcccgcacc gtgcatgcgg gctgacccct cccataaaaa attcctatga
     2461 aaaaaaatct ttattccttt atctttcttc ttcttattgg agcacttcta agcgcttgct
     2521 ctcttccttc tgccgctgaa actcaatctg ctcaacccgt aaatgacccc aatgctgtaa
     2581 tcccacaaag tgtggcaacc gcctctgtcg taacaacctc atccctaccc gccggcatgc
     2641 caactgttga aacagacctt agcaaaatac taagtgtttt acagagtggg aacttctctt
     2701 atcttgcaaa tctcgcttca gaaaaatatg atgccagcgc atatacggtt cccggctccc
     2761 tagtcttcaa tgcagccttt cccagcagcc aaaccattta tctaaattat ggctggtgcg
     2821 caaaagacct cgcaacctta caacagaacc ttcaacatat ttccacgtac ttctctttta
     2881 atggtcaaca aatccccatg gaatatgtga acaccatcag cacagaaagc agcgacgggt
     2941 tgcaatgttc caatacgggt ttactgcttt cagggtgggc acccggcacc taccaattcc
     3001 aagtcaaagc cagctttgat tcacaggtca acgatggcag tgcggattac cccgcaggag
     3061 attacatttc agaatacacg gtcaccgttc aataaaccac actcaccccc gaagacagcc
     3121 tttcgcgggt gagttttcat ttaattacag ttttattagc cc
//
LOCUS       DAOQFT010000286         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_18076_length_3159_cov_6.861469, whole genome shotgun sequence.
ACCESSION   DAOQFT010000286 DAOQFT010000000
VERSION     DAOQFT010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18076_length_3159_cov_6.861469"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..480
                     /locus_tag="PLR93_08050"
     CDS             253..480
                     /locus_tag="PLR93_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26955.1"
                     /translation="MSVQTLENTMEKCETTTSDAAPAETPARSLFTRQTGQALITLGT
                     LFKHVPPFNESIDKSTGDTLISIGKKLKGKK"
     gene            553..1665
                     /locus_tag="PLR93_08055"
     CDS             553..1665
                     /locus_tag="PLR93_08055"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH26956.1"
                     /translation="MAKKNNSSPKLVWDFGTAYELFISLHVLLEPEHFGIRPSYAASV
                     RARIPAAERKLLEELYPLLGVPLKWLNTLPGPKDAISALWAIKQIPPAKRMIELYNVH
                     EVYDADKPEDVEKHKTFREILLRIAAEGKVLSTDVDFFQKLFSQKHGNMKREAVESAL
                     DWWSRPAELGEAFLTAFQSYYQAFFEEEEKRIEPILRAGLERAQALSSTVSFEELFTE
                     LSQGLQVGDEFNAPNYIIVPAFWITPLIFFERFDRNTMFLTFGARPADMSVIPGETVP
                     DALVRSLKALADPTRLKIMHYLTHESLTPSEIARKLQLRPPTVTHHLKELRLAGLVEL
                     SLMHEENRYTARKQALDAVYEHLNAFLQGENLQELA"
     gene            1662..2942
                     /locus_tag="PLR93_08060"
     CDS             1662..2942
                     /locus_tag="PLR93_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26957.1"
                     /translation="MNTTTSFKPFRILGEYSSHIRAFKPNARLYLLNVIITGAVMGVF
                     RLIFNFYALSLGFDESVLGNLITTSSFVALIAALPMGYLADIIGRKGSLILSSILLAF
                     SVIAMALWKTETSLYAMNVVSGLAQSLAGVTMSPFLMENSDEKERTYLFSFGQGLQMT
                     MASIGNWVGGYLPTWISQTQHVSETSSIAYGNSILIAGIGALIAVLPLMFIKSPNITR
                     SQRAVFAPFQYAAKNPGLLTKLVLPMLLTSLGAGLIMPFMNVFFRVVHHQPDPVIGTL
                     FAWGSLAMGIGLLIAPPLADRTGKIQLVVITQALSIPFLILLGFSPIFWVGAASYYIR
                     LALMNMSSPVYQTFVMEHVEPSGRATVASLTSMAWNFGWAFSPTISGWLQVKYGFGPA
                     FIGTITLYTISVLMYWAFFWRRSIDPVPASAAAD"
BASE COUNT          787 a          914 c          674 g          784 t
ORIGIN      
        1 ccaccaaact cgccaacaaa atgatggaac tgagtccaac cagcaccccc gccaagattt
       61 ttcgcttcat ggttccgtcc tttcatatag tagaaccctg ccgctgccaa atcagaatga
      121 cccaaaactc ctatttgact agaaataccc cctacctact tgacaaagcg tctaagttag
      181 actattatct aattagatat ccgtctaata cagtcatttg cctaaccgtc cccatcagag
      241 agagaggaca ccatgtcggt acaaacctta gagaacacaa tggaaaaatg cgaaaccacc
      301 acatcagacg ccgctccggc tgaaacccca gcccgaagcc tcttcacccg ccaaaccggg
      361 caggcgctca tcacgcttgg caccctgttc aaacatgttc ccccattcaa tgaaagcatc
      421 gacaaaagca ccggggatac tctcatctcc atcggtaaaa aactaaaagg aaaaaaataa
      481 agcccacact tcaggtgaca gtccaatcga tgggctgtca cctgaacttt gaatacgggc
      541 acactactat atatggctaa aaagaacaat tcctctccaa aactcgtttg ggattttggc
      601 accgcctacg agttattcat cagcttacat gttctgcttg aacccgaaca tttcggcatt
      661 cgcccatctt atgccgccag tgttcgagcc cgtattccgg cagcagaacg aaaactgtta
      721 gaggagttgt atccgctttt gggtgttccg cttaaatggc tgaataccct gcccggaccc
      781 aaagatgcca tcagcgcact ttgggcgatt aaacaaattc cgcccgccaa acggatgata
      841 gaactgtata acgttcacga agtgtacgat gccgataagc cagaagatgt tgaaaaacac
      901 aaaacctttc gagagattct gttgcgcatc gcggctgaag gcaaagtcct ttccaccgat
      961 gtagattttt tccaaaagct cttctcgcaa aaacacggca acatgaaacg cgaagccgtt
     1021 gaatccgctt tagattggtg gagcagaccc gccgaactcg gtgaagcgtt cctcactgct
     1081 tttcaatcct actatcaagc attttttgag gaggaagaaa agcgcatcga acccatcctg
     1141 cgtgctgggt tagaaagagc ccaagcttta tcttccaccg ttagctttga agaattattt
     1201 actgaactct cgcagggtct tcaagtgggt gatgaattca atgcccccaa ctacatcatc
     1261 gtccctgcat tttggatcac cccgctaatc ttcttcgaac gctttgaccg caacaccatg
     1321 ttcctcacct tcggcgcccg ccccgccgat atgtctgtca tccccggcga aaccgtaccc
     1381 gatgccctag tccgttcgct caaagccctt gcagacccca cgcgcttgaa gatcatgcat
     1441 tacctcacac acgagagcct gaccccttct gaaatcgccc gcaagttgca gctccgccca
     1501 cccacagtga cgcatcacct caaagagctt cgtctcgccg gtttggtgga actctctctc
     1561 atgcacgaag agaaccgtta caccgctcgc aagcaagcgc tcgacgcagt gtatgaacat
     1621 ctaaatgcct tcctgcaagg cgaaaatcta caagaactcg catgaacacc acaacctctt
     1681 tcaaaccctt ccgtatcttg ggagaatata gctcccacat ccgcgcattc aaaccgaatg
     1741 cgcggcttta tcttctaaac gtcatcatca ccggtgcagt gatgggcgtt ttccgcctca
     1801 tcttcaactt ctacgccctg agccttggct tcgatgaatc ggtgttgggc aatctcatca
     1861 ccaccagcag tttcgtcgct ctgatcgccg ctctgcccat gggctatctg gcagacatca
     1921 tcgggcgcaa aggttcgttg attctttcca gtatcttgct ggcattttct gtcattgcca
     1981 tggcgctatg gaaaaccgaa acatcgctct atgccatgaa cgtcgtttct gggttggcac
     2041 aaagtttggc aggcgtgacc atgtctccct tcctcatgga gaatagcgac gagaaagagc
     2101 gcacctacct cttcagcttt ggtcagggct tgcaaatgac catggcatct attggcaatt
     2161 gggttggcgg ctacctcccc acttggatca gccaaaccca acacgtctct gaaaccagca
     2221 gcatcgcgta tggaaattcc attctcatcg ccggaatcgg cgcattgatt gctgtgttgc
     2281 cattgatgtt cattaagtca ccgaacatca cccgaagtca acgcgctgtt tttgcacctt
     2341 ttcaatacgc ggcgaagaac cccgggctgc ttacaaaact tgtcctgcca atgttgctga
     2401 cctcgttagg tgcgggtctc atcatgccat tcatgaatgt cttcttccgc gtggttcatc
     2461 accaacccga cccagtcatt ggcacgcttt tcgcttgggg ttcactggca atgggcattg
     2521 gtctgcttat cgcgccacct ctcgcagacc gaacaggcaa aatccaactc gtggtaatta
     2581 cacaagcgct ttccattcca tttctcatct tgcttggttt ctcccccatc ttttgggttg
     2641 gcgctgccag ctattacatc cgcctcgcgc tcatgaacat gagcagcccc gtttaccaaa
     2701 ctttcgtaat ggaacacgtg gagccatcag ggcgtgccac ggttgcaagc ctcaccagca
     2761 tggcatggaa cttcggctgg gcgttcagcc ctaccatcag tggatggttg caagtgaagt
     2821 atgggtttgg tccggcgttc attggaacca tcacacttta tacgatctcg gtcctcatgt
     2881 attgggcatt cttctggcgc cgaagcattg acccggtccc agcttcggca gccgcagatt
     2941 agaaaagtct caccgatttt ggaaaaacag agcattgaaa ttgctctgtt tttttataaa
     3001 atatcttaat ggacaaagat atcttgatta ttggcggcgg tccgagcgga ctctcgactg
     3061 cccttcatct cgcaaaatat gccccgcatc tgactcagcg tattttgatc ctcgaaaagg
     3121 aacattatcc acgcctgaag ctttgtgctg gcggcttgg
//
LOCUS       DAOQFT010000287         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_18154_length_3151_cov_6.803295, whole genome shotgun sequence.
ACCESSION   DAOQFT010000287 DAOQFT010000000
VERSION     DAOQFT010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18154_length_3151_cov_6.803295"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1068
                     /locus_tag="PLR93_08065"
     CDS             70..1068
                     /locus_tag="PLR93_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH26958.1"
                     /translation="MKNTTEKLLEIRGLKKYFEAGRPSLFSRETKHVHAVDGVNLTLR
                     RSEVIALVGESGCGKSTLGLLLMGLEDPTDGSVIFEYRDITHLNDHQRKDLRRKIQMV
                     FQDPYESLNPTQTIEEIVTEPLTVHGMASSHQDRDERVRKAMEDAGLKPADVYLHRYP
                     HELSGGQRQRVVIASALVLEPEIIIADEPVSMLDVSIRAEVINLLAELRISRQIAVIF
                     ITHDLGSVGFFADRVAVMYLGRIVEIGTMLEVLEKPQHPYTKALLSVIPVPNPRLRHE
                     RIVLQGETPNPINIPSGCRFHPRCPAAFDACKLSDPPMTTLSKSHQAACLLVTNTK"
     gene            1069..1842
                     /locus_tag="PLR93_08070"
     CDS             1069..1842
                     /locus_tag="PLR93_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNH26959.1"
                     /translation="MDITSLQNPRVKHIVKLREDKKQRQKDRLILVEGYDELTLALSC
                     GFEPQTLLSAPELVSQSLTVPNAENLTVSRAVFEKISYRDNPDGWLGLFPMPTSSLDE
                     LKLSENPLVIVAESVEKPGNLGAILRTADAAHVDAVLVCDPRVDTWNPNTIRASRGAV
                     FAVPVVEVKSAEALSWLRSRKMRVLAATPSADAMYTDVDMQGPLAIAVGTEDEGLTDF
                     WMQNSDLRVKIPMTGKVNSLNVSIATALMTYEAVRQRAK"
     gene            1839..>3151
                     /locus_tag="PLR93_08075"
     CDS             1839..>3151
                     /locus_tag="PLR93_08075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH26960.1"
                     /translation="MSSKPARQNSRSETAVFANLGRDLASTTEARSAAEIILNAADSL
                     IGWDAAYLILYDPAIGGVPRPLLTIDTINGNHVEQKNAMPQKPSENMLKAIAHGGFMS
                     HYDEPFDIGVTASFGDRSKRTLSQLFVPVVSGSRTIGVLSIQSYQKYFYKDEQLELLK
                     DLASHCAGALERIWAQEAVNDLVERLKVLHAAVNAINASLEMERVCQVVYETVKQVMP
                     CDDFVIDGYDKDTNEIVPIYAIEYPGKRVFTNRYLADHGMAGEIVLSKKSLLFNSVEE
                     MDNSGIKFELYGTHEDDPSQSILAVPMTLHGDMYGMVSAQSYNTNAYNRDHLYLLEVL
                     ASHAAIAIENARLFDSIQNLANTDSLTKILNRRRFFELAEQEFVRAEQFNIPFSVIMI
                     DVDDFKQFNDKHGHKIGDAVLILAAETLKSSLRTDDILGRIGGEE"
BASE COUNT          838 a          725 c          747 g          841 t
ORIGIN      
        1 agaagaaccc cttcttcata ccatcgcaaa cgggcatgta gggagctgcc acctgattga
       61 gaaatcaaaa tgaagaatac taccgaaaaa cttttggaaa ttcgtggtct gaaaaaatat
      121 tttgaagcgg ggcgcccatc cttgttttca cgcgaaacga aacatgtcca cgctgtggat
      181 ggtgtgaacc tgaccttacg tcgcagcgaa gtaattgccc tcgttggcga aagcggatgc
      241 ggcaaatcga ccttgggttt gttgctgatg ggtttggaag accccaccga tggttcggtt
      301 atttttgaat atcgcgatat tacacatctc aacgaccatc aacgaaaaga cctacgccga
      361 aaaattcaaa tggtttttca agacccctat gagtctttga accccacgca gaccattgaa
      421 gaaatcgtaa ccgaaccgtt gaccgtgcat ggtatggcat catctcatca agatcgtgat
      481 gaacgtgtgc gaaaagccat ggaagacgcc gggctaaaac ctgcagatgt atatttgcat
      541 cgttacccgc acgaactttc aggagggcaa cgtcaacgcg tggtcattgc atctgcgctt
      601 gtgcttgagc cagagatcat tattgccgat gaacccgtgt ctatgttgga tgtatccatc
      661 cgcgcagaag tgatcaacct tctggcagag cttcgcattt cacgtcagat cgcagtcatt
      721 tttattacgc atgatcttgg ctcggttggc ttctttgctg accgtgtagc agtgatgtat
      781 ctcggacgaa tcgtagagat tggtacaatg ttagaagttc ttgagaaacc acagcatccg
      841 tataccaaag cgttgctctc ggttatccct gtgcccaatc cgcgtttacg gcatgaacgc
      901 attgtccttc aaggtgaaac gcccaacccc attaatatcc cttcgggttg tcgtttccat
      961 ccgcggtgcc cggcagcctt tgatgcctgc aaactcagtg acccgcccat gacaaccctt
     1021 tcaaaatcac atcaggcagc ttgtctgtta gtaacgaata caaaataaat ggatattaca
     1081 agtttacaaa acccgcgcgt caagcatatt gtcaaattgc gtgaggataa aaagcagcgc
     1141 caaaaggatc gcttgattct ggtggaaggc tacgatgaat taactctcgc gctttcgtgt
     1201 ggattcgaac cgcaaacgct tctttccgcg cccgagcttg ttagccaatc cctaactgtt
     1261 cccaacgctg aaaatttgac agtgagccgt gccgtctttg aaaagatttc ctaccgtgat
     1321 aaccccgatg gttggcttgg gcttttccct atgccaacat ccagcttgga tgaattgaag
     1381 ctttcagaaa atccgctagt gattgtcgct gagtctgtag agaagcctgg taatcttggc
     1441 gccatccttc gcactgccga tgccgcgcat gtagatgcag ttctcgtctg cgacccgcgt
     1501 gtggatacgt ggaatccaaa caccattcgc gccagccgcg gagcagtctt tgccgtacct
     1561 gttgtcgagg tgaaatcggc tgaagcgctg agttggttga ggtcccgtaa gatgcgtgtt
     1621 ctggctgcaa ctccatctgc cgatgcaatg tacacagatg ttgatatgca aggtccgctt
     1681 gccattgcgg ttgggacaga agatgaaggt ctcaccgatt tctggatgca gaattcagac
     1741 ctgcgtgtaa aaattcccat gacgggaaaa gtaaactcct tgaatgtttc gattgccact
     1801 gcactaatga cttatgaggc agtacgacaa agagcaaaat gagcagcaag ccggcacgtc
     1861 agaattctcg aagtgaaaca gccgtttttg caaacttggg gcgtgatcta gcatcgacga
     1921 ctgaagcaag gtctgcagca gagatcatat taaatgccgc agatagcctt attggttggg
     1981 atgccgctta tcttatcctt tatgaccctg ccatcggtgg tgttccacgc ccattgctta
     2041 caatcgacac tattaatgga aaccatgtag aacaaaagaa tgccatgccg caaaaaccca
     2101 gtgaaaatat gctgaaggca attgcgcacg gcggctttat gtctcactat gacgagcctt
     2161 ttgatattgg agtaacggct tcattcggag atcgaagtaa aaggacttta tctcaattgt
     2221 ttgtgccggt cgtcagcggt tcgcggacaa ttggtgtgct ttccattcag agctatcaaa
     2281 aatattttta taaagatgag caacttgaac tgttgaaaga tctagcaagc cattgcgccg
     2341 gagccttgga acgtatttgg gcgcaagagg ctgtaaatga tttagtcgaa cgcttgaaag
     2401 ttcttcatgc cgccgttaat gccatcaacg caagccttga aatggaacgg gtttgccagg
     2461 ttgtatacga gaccgttaaa caagtcatgc cgtgcgatga ctttgtaatt gatggctatg
     2521 ataaagatac gaacgagatt gtgccaatct atgctattga ataccccggt aagcgagtct
     2581 ttacgaatcg atatcttgct gaccatggta tggctggaga gattgttctt tccaaaaaat
     2641 cactgctttt taacagtgtt gaagaaatgg acaatagcgg catcaagttt gaactttatg
     2701 gcacgcatga agatgacccg agtcagtcta ttttggctgt gccaatgacc ttgcacggtg
     2761 atatgtacgg tatggtctcg gcacaatcgt acaatacaaa tgcttataac cgagaccatt
     2821 tatatttatt ggaagttctt gcgtctcatg ctgccattgc catcgaaaat gcccgtttat
     2881 ttgattccat ccaaaatctg gcgaatacag actcgcttac gaagatcctc aatcgccgtc
     2941 gcttttttga attagctgaa caggaatttg tccgtgctga acaattcaat attccttttt
     3001 cagtcatcat gatcgatgtg gatgatttca aacagttcaa cgacaaacac gggcacaaaa
     3061 taggggatgc ggttttgatc cttgctgctg aaacgctaaa gtcttctcta cgtaccgatg
     3121 acattcttgg gcgtattggc ggagaagaat t
//
LOCUS       DAOQFT010000288         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_18210_length_3143_cov_6.524288, whole genome shotgun sequence.
ACCESSION   DAOQFT010000288 DAOQFT010000000
VERSION     DAOQFT010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18210_length_3143_cov_6.524288"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..847
                     /locus_tag="PLR93_08080"
     CDS             152..847
                     /locus_tag="PLR93_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26961.1"
                     /translation="MKKALTITLALLLTACGVVQPATQEVLPTPIVQTVVVIATSEAT
                     QPLPPTAISLPTQGPTNTAEPVNTATTAPTETLVPTSAPVATEVVPTLSSSSTTELKP
                     VNVSNDLGKGVFKDITFSSDLLTLQCYPREMTITITANLPEIVSAIMYYRMVDNPDAL
                     YPSEWKNLGEMVSDKQGHFSFVLESLDLNPDLRVLEKAWIDFQFIGINKGGGVVDRTQ
                     KIEKMVTYYKACP"
     gene            complement(889..1146)
                     /locus_tag="PLR93_08085"
     CDS             complement(889..1146)
                     /locus_tag="PLR93_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26962.1"
                     /translation="MIRTIVFKTVASILLMLGALGLSMPMNAYAQSGTQHIQSAQYSG
                     KCEKIKDKKQKKACEKAQKAGKTKKNDDGPNHESNDGANHH"
     gene            complement(1270..2586)
                     /locus_tag="PLR93_08090"
     CDS             complement(1270..2586)
                     /locus_tag="PLR93_08090"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNH26963.1"
                     /translation="MKTIQRFLAMFVLVALFSLTFASPALAFDGRSGDKIVIAADEVI
                     NDDLYVSAEEFTLDGTVKGDLVVFGAYIFINGTVEGDLIAAGNTIVIDGTVKDDARIA
                     GAALQLGEKASIGGDLISAGASLEAQNGSAVGQDVVFAGGQSLLAGSIGRNLWVGTGA
                     LELSGEIGGDVQAEVGEMQEGSPSPSMYIGDVKVSTPSVQPGLTVTESAKIGGDFTYT
                     QSKDIKFPSDAIEGKVTRKEPTVDTQSVPTPPTAGEVAATWVFDLLRKIATLAIFGLL
                     LVWLVPGFLKGLGDKLQSQPIPSFGWGIVAYAAFFFAILLIVIVMIVGGIIFGVISLG
                     GVTGTIIWVGLLSIFAMIVGFALFTGFGAQILVAWLGGKWILGRFNPALAEHKVWPLL
                     LGVVIVGLLVKLPLVGWLIGFLIMFFGLGALWLWGRERMKKPEAVA"
     gene            complement(2718..>3143)
                     /locus_tag="PLR93_08095"
     CDS             complement(2718..>3143)
                     /locus_tag="PLR93_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH26964.1"
                     /translation="VHVFLFNEQGEMLIQKRSADRANSPSMWDCSVSEHVKAGESYLD
                     AAMRGLKEEMGVEGIEISLRGKIQMEYGFNDNEISEIYEGRLNGKFVQFDPVEISEVR
                     FVSLDVVKVGTFQSPELYCYWFIQLMNWYFGLPAKLKEL"
BASE COUNT          886 a          794 c          645 g          818 t
ORIGIN      
        1 ggttggaccg gtggggatgt gcttgtggct aggtgtactt tgcgtttctt tgcagaattg
       61 ataaggaatc cgtctttgaa atagccccaa ggggtcaact aggaaaaatg gacttgggca
      121 tagaatggag taaatttaca aaggagtttt catgaaaaaa gcactcacta ttaccctagc
      181 cctgctcctg acggcatgcg gcgttgtaca gccagccact caggaagtat tgccaacgcc
      241 gattgtgcaa actgttgtgg ttattgctac atccgaagct acacaaccgc ttccgcctac
      301 tgcaatttca cttcccactc aaggaccgac aaacactgct gagcctgtta atactgcgac
      361 taccgcccct actgagacat tggtacctac ttctgcccca gtagcaactg aggttgtacc
      421 gactctttcg tcttcatcga ctacagaact gaagccagtt aatgtaagta atgatttggg
      481 caagggcgtc ttcaaagata ttaccttttc aagcgatctt ttgacacttc agtgttatcc
      541 gcgtgaaatg accatcacta ttaccgcaaa tcttccagag attgtaagtg caattatgta
      601 ttatcgtatg gtcgataacc ctgatgcgct ttacccctct gaatggaaga accttggcga
      661 gatggttagc gacaagcaag gacatttttc gtttgtcttg gaatcgctag atttgaaccc
      721 ggatttacgc gttcttgaaa aagcttggat tgatttccaa tttattggta tcaataaagg
      781 tggtggcgtt gtagaccgta cccagaagat cgaaaagatg gtgacttact acaaagcatg
      841 tccgtaatag ataataaaaa agcgacccca cttggggtcg cttttttatt agtggtgatt
      901 ggctccatca tttgattcat gatttggtcc atcgtcgttt ttcttagttt tgcctgcttt
      961 ttgggctttc tcacacgcct ttttctgttt cttgtctttt atcttttcac atttaccgct
     1021 gtattgagca gattgaatat gttgtgtgcc agattgtgca taagcgttca ttggcatgct
     1081 tagacccaaa gctccaagca tgagaaggat ggatgcaacg gtcttaaaaa cgatagttct
     1141 gatcatggta ctctcctatt tactggtgat ttaataggat agtcggtagt atttgataaa
     1201 agttatgggt gtgaattaaa gcacaagcgg cgaggaactt cctcgccgct tgtatgtgcg
     1261 atttatttgt caagcaaccg cttcaggctt tttcattcgc tcacgtcccc agagccagag
     1321 tgcgcccaaa ccgaagaaca tgattaagaa tccgatcaac caaccgacca aaggtaattt
     1381 gaccaataag ccaacaatga ccacgccgag caaaagaggc cagaccttgt gttcagccaa
     1441 tgcggggttg aagcgaccaa gaatccattt gccgcctaac catgcaacaa ggatctgagc
     1501 accgaaaccg gtaaacaaag cgaacccaac aatcattgcg aaaatgctca gcaaaccaac
     1561 ccagatgatg gtaccagtta caccaccaag agagatcaca ccgaagatga tgccgcctac
     1621 aatcattacg attacgatca ataggattgc gaagaagaaa gcagcatagg caacgatgcc
     1681 ccagccaaag ctcggaatag gctgactctg aagcttatct ccaagacctt tcaagaagcc
     1741 tggcacaagc cagaccaaca aaagcccaaa gatggcaagc gttgcaatct tgcgcagcaa
     1801 gtcaaatacc catgtagctg caacttcacc tgctgttggc ggagtgggaa cactttgcgt
     1861 atcaacagtc ggttctttgc gagtgacttt tccttcaata gcatcagaag ggaacttaat
     1921 gtccttcgat tgggtgtatg taaaatcacc accaattttt gcactttcag taacggtcaa
     1981 accgggctga acgctaggcg tagaaacctt tacatcccca atatacatgg aaggagacgg
     2041 gctgccttcc tgcatttccc caacttcagc ttgcacatcc ccgccgatct caccactcaa
     2101 ctccaaggcg ccagtaccca cccagagatt acgtccgatc gaaccagcga gcaaactttg
     2161 tccgccagca aaaacaacat cctgccccac agcactgcca ttttgggctt caaggcttgc
     2221 accagctgaa attaaatctc cgccgataga cgccttctca cccagctgta aagccgcacc
     2281 ggcaatacgg gcatcatctt ttacagtgcc atcgatcacg atcgtatttc ctgcggcgat
     2341 caaatcacct tccacggtac cattgatgaa gatataagca ccaaacacca ccaaatcccc
     2401 tttgacggtt ccgtctaaag tgaactcttc tgcgctaaca tataagtcat cattaatgac
     2461 ttcatctgcg gcaataacga ttttatcgcc cgaacgccca tcaaatgcca aggcaggcga
     2521 ggcaaatgtt agagagaaca aggcaactag gacaaacatt gccaaaaatc tttgaattgt
     2581 tttcataaat actcctttga tagaaacttc ctgaggagga tatacgcccg aatatcccaa
     2641 aggttgcaga gatgcgccct cgcctctttc tcagctcgta gttgacataa cctattgacc
     2701 tttaatgact ttgaagccta tagttccttc aattttgcgg gcagaccaaa ataccaattc
     2761 attaattgga taaaccaata acaataaagc tcaggggatt ggaaagtccc aaccttgacc
     2821 acatccaaac ttacgaatcg aacttcacta atctctaccg ggtcaaactg cacgaatttt
     2881 ccattgagcc tgccctcgta aatctcactg atctcgttat cattgaaacc atattccatt
     2941 tggatcttcc cacgcaagga gatctcaatg ccttccacgc ccatttcttc ctttaaaccg
     3001 cgcattgccg catcgagata actttcccct gccttcacat gctccgacac cgagcaatcc
     3061 cacattgatg gtgagttggc acggtctgca ctgcgttttt ggatgagcat ttcaccctgc
     3121 tcattaaata gaaagacatg cac
//
LOCUS       DAOQFT010000289         3135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_18274_length_3135_cov_7.373701, whole genome shotgun sequence.
ACCESSION   DAOQFT010000289 DAOQFT010000000
VERSION     DAOQFT010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18274_length_3135_cov_7.373701"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1015
                     /locus_tag="PLR93_08100"
     CDS             179..1015
                     /locus_tag="PLR93_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNH26965.1"
                     /translation="MKLGIVTDSTADLPSYLIEQHEIQVVPTVLILEGKEYKDGIGIS
                     REEFYNRLPALRTPATTAAPSIGDFLTPYQSLLNSGCDHILSIHVSGKLSGVVNIARR
                     AAEEFPGKVTCVDSGSLSMGIGFQVLAAAESADLGVKVALEALASTERRTKVFAALDT
                     MEYLKRSGRVPAVVANIGGLLSIKPVVELRNGEVKPMAINRTTSQADEFILGKLLEVG
                     EMERLAILHTNAEDRAQRLLQAMMERSHMAVPRDVLYVNVTAVIGTHLGPHCIGFAAV
                     KK"
     gene            complement(1059..2006)
                     /locus_tag="PLR93_08105"
     CDS             complement(1059..2006)
                     /locus_tag="PLR93_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH26966.1"
                     /translation="MNWFNNSRLFELARQGKRLTHIAVVIPLSFIFVVLAQLGIIPLA
                     FIMQAVYGFEDMFQVSLKGFPNTVIGFWMGMQLVFAFGLTYFILWAWLKWFEKRPFWT
                     LGYETKDALSKYGRGFLFGALMFAGAVGILALFGSVTFEQGDPAYQGLPALGGVFLVL
                     IGWIVQGGSEEIVMRGWVLPVIGARYKPWLGLVMSSLIFAILHGLNPNLSVIAMINLA
                     LFGVFAGLYVMREGSLWGISALHTVWNWVQGNFFGLEVSGNGAGGGTLINLVESGADW
                     LTGGQFGPEGGLAVTIVLAIGILVILFWKNKATVQEISA"
     gene            2139..>3135
                     /locus_tag="PLR93_08110"
     CDS             2139..>3135
                     /locus_tag="PLR93_08110"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNH26967.1"
                     /translation="MPAMIKLTIMQPSLEKLRKFFRLEHENKYENTAIIGGLAKMLDY
                     WEGEARADGVAEEVIQAVVSRLRAYEKLNPTGRADSLKGLWKRISETYPEAGQKPKAP
                     PPPQRPNPQPQRPQRSDEEAKPKADAAEQPAAQNQSQPKQHPQQHQQKQQRPNNSPQR
                     FESATSAKHSQTPAALGAKLTVLNGVGPKNAESLAKLGMQTLGDMLYSFPRRYEDYSL
                     LKPIQDLMYNDVVTVLGTIQSVHNRPIRGGKQSIIEVIISDGTGSLRVSFFNQPWLAN
                     RFKTGDAISVSGKIDQYLGRIVMNSPDWENVEMESLSTNRIVPIYSLTERITQKWL"
BASE COUNT          870 a          763 c          755 g          747 t
ORIGIN      
        1 aaaacaacat tttcgagaga ttctgtgttt gccatatctc atcctttatg gcattgtact
       61 gtgtaatata cagtatgtca agattaaagt atcaggtgac cttcacgcta tttttagggg
      121 ctcgtgacgc aagttcatgg tgaacaacgg tatatccaaa tatgctacac tttgccctat
      181 gaaactagga attgtcaccg actcaaccgc agacctgcca tcgtatctaa tcgaacaaca
      241 tgagatccag gtggtgccta cagttcttat tttggaagga aaagaatata aggatgggat
      301 cggcatctcg cgcgaagagt tttataaccg cctgcctgcg cttcgcaccc cggcgacaac
      361 tgctgcgccg tccattggag attttcttac gccgtatcag tctctgctca actccggctg
      421 tgaccatatc ctgtcgatcc atgtttcagg caaattatcg ggtgtggtaa atattgctcg
      481 ccgcgccgcc gaagaattcc ccggcaaagt cacctgtgtg gacagtggct cgctttcgat
      541 gggcattggc tttcaagtgc ttgccgccgc cgaatccgcg gaccttggtg tgaaagttgc
      601 gttggaggca ctcgcatcga cagaacgcag aacgaaggtt ttcgccgcgt tagatacaat
      661 ggaatatctc aagcgcagtg gacgcgtccc ggcggtggtt gccaatattg gcggcttact
      721 ctccatcaag ccagtggtgg agttgcgcaa tggggaagtt aaaccaatgg caattaaccg
      781 cacaaccagt caggcagatg aatttatatt gggaaagttg ttggaagtgg gggagatgga
      841 gcgtttagcg attttgcata ccaacgctga agatcgtgcc cagcgtttat tacaagctat
      901 gatggaacgt tcgcatatgg cggtgccaag agatgtatta tatgtaaacg tgacggcagt
      961 tattggcaca catttaggtc cgcattgtat tggatttgcg gcggtgaaga aataaaaaag
     1021 ctcgcttgag tctaaactca agcgagcttt taggtttact acgcagatat ttcttgaacg
     1081 gtcgctttgt tcttccaaaa cagaatgacc aaaattccga ttgccaacac aatcgtaaca
     1141 gccaagccgc cttcgggtcc gaactgaccg cccgtgagcc aatctgcgcc actttctacg
     1201 aggttgatca gagtgccgcc gcctgcgccg tttccgctca cttccaagcc gaagaagttc
     1261 ccttgtaccc aattccaaac cgtgtgcaag gcgctaattc cccaaagtga gccttctcgc
     1321 ataacataca aacctgcgaa taccccgaac aatgccaggt tgatcatggc aatcacgctt
     1381 aagttggggt taagtccgtg cagaatggcg aagattagcg aggacatgac cagccctaac
     1441 caaggtttgt agcgagcacc aattactggc aacacccatc cacgcataac tatttcttca
     1501 gaaccgcctt gaacgatcca accgatcaac acgagaaaaa cgccacctaa ggcgggcagc
     1561 ccttggtaag ctgggtcgcc ttgttcaaag gttacactcc caaacaaggc aaggattcct
     1621 accgcaccag caaacatcaa tgcgccgaat aaaaatccgc gcccgtattt ggacagggca
     1681 tctttggttt catacccaag ggtccagaat gggcgctttt caaaccactt gagccatgcc
     1741 cagaggatga aataggtcag accgaaggca aagacgagct gcatccccat ccaaaaacca
     1801 atcactgtat ttggaaagcc ctttaacgat acttggaaca tatcttcaaa gccataaacc
     1861 gcctgcataa taaatgcgag cggaataatg cccagctgtg ccagcaccac gaaaatgaag
     1921 gaaagcggaa tgaccactgc aatgtgagtg aggcgctttc cttggcgcgc cagttcaaaa
     1981 agcctgctgt tgttgaacca attcatgatt ttctcctaat tcagtactaa taatttgata
     2041 atagtgtcaa tttgttatta agtcaagccc tttcagtagg ttttttgggg gataagtatc
     2101 aaattcaaag ctattcgagg gatattgaga atcggttcat gcctgccatg ataaaattga
     2161 ctattatgca accgtctttg gaaaaactac gcaaattttt tcgactcgaa cacgaaaaca
     2221 aatacgaaaa taccgccatc atcggcggac ttgccaaaat gctggattat tgggaagggg
     2281 aagcccgcgc cgatggcgtg gcggaagaag ttattcaagc tgttgtttcc cgtttacgcg
     2341 cttatgaaaa gttgaatccc accgggcggg cggattccct taagggcttg tggaagcgca
     2401 tcagtgaaac ctacccggaa gcagggcaaa agcccaaagc gccaccaccg ccgcagagac
     2461 cgaatccaca gcctcaacgt ccgcagcgaa gcgatgagga ggcgaagcca aaagcagatg
     2521 ccgctgaaca acctgcggcg cagaatcaaa gtcagccgaa gcagcatcct caacaacacc
     2581 aacaaaaaca gcagagacca aataattctc cgcaacgttt tgaatctgcg accagtgcga
     2641 aacatagcca aacacccgct gcgttgggtg ccaagcttac ggtcttgaat ggggtgggac
     2701 cgaagaatgc tgagtcgttg gcaaagcttg gaatgcaaac gctgggggat atgttgtatt
     2761 ccttcccgcg tcggtatgaa gattattcac tgctgaaacc gattcaagat ttgatgtaca
     2821 acgatgtggt gactgtgtta ggcaccattc aaagtgtaca taaccgtccg attcgcggtg
     2881 gaaagcaaag cattatcgaa gtcatcattt ctgatggcac aggctcattg cgagtctcgt
     2941 tcttcaacca accttggttg gcgaatcgtt tcaagacggg cgatgcgatt tctgtttctg
     3001 gaaagatcga tcaatatctt ggtcgcattg taatgaacag ccccgattgg gaaaatgttg
     3061 agatggaaag tctcagtacc aatcgtattg tgccgatcta ttcgctcact gaacgcatca
     3121 ctcaaaaatg gttgc
//
LOCUS       DAOQFT010000290         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_18356_length_3127_cov_6.517904, whole genome shotgun sequence.
ACCESSION   DAOQFT010000290 DAOQFT010000000
VERSION     DAOQFT010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18356_length_3127_cov_6.517904"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLR93_08115"
     CDS             <1..1131
                     /locus_tag="PLR93_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH26968.1"
                     /translation="VRMHRPVTHLTKIYIEPTVACNLDCITCFRNAWDQPIGKMTDET
                     FESIFNSLKQMDPIPSVYFGGIGEPLFHPKTIEWIRRIKQELGVKVELITNGTTLTER
                     KTRELIDAGLDILWVSLDGATPESFSDVRMGAELPVILENLRRLFRMRNGGHFPQPEI
                     GVAFVAMKRNINDLPKVIKLGHTFGARYFSVSNIQPATEGMQEDRLYKRTIRNIAYLP
                     SPVLPKLSLPKMDFNEETQAALTEAFNSGCNVSFAGNNWGGANDVCNFVESGTMSIAW
                     TGEVSPCWPLMHTHMSYLHGKPRLSKKHVIGDTRKQSLEEIWLSPEYVAYRERLHNFV
                     FAPCTFCGGCDLSETNEEDCLGNDIAPVCGGCLWAQGIIQCP"
     gene            1210..1542
                     /locus_tag="PLR93_08120"
     CDS             1210..1542
                     /locus_tag="PLR93_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNH26969.1"
                     /translation="MQLRFYANMRTTVGMPLLEINEAMVDSFRKLIPLLLEQYPETAF
                     HLLDEHGELRPDVPVYVDGRNPRLSSVGMDTPLKPDSVISFFSPISSGKLNVEVLREP
                     TIDTRSEP"
     gene            1539..1817
                     /locus_tag="PLR93_08125"
     CDS             1539..1817
                     /locus_tag="PLR93_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNH26970.1"
                     /translation="MKVNFFATLRDIAGGKTVEFDLDHGITARELLDAICERFPAMKK
                     ELLKEDGEMHGHVHFFINGRDVQFTDLQFATPITQGDTVNVFPAVGGG"
     gene            1959..2963
                     /gene="moaA"
                     /locus_tag="PLR93_08130"
     CDS             1959..2963
                     /gene="moaA"
                     /locus_tag="PLR93_08130"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNH26971.1"
                     /translation="MTILDTLNRPLRDLRISVTDRCNFRCVYCMPKEIFGSDYKFLHH
                     SQVLTFEEITRIARLFAGLGVKKIRLTGGEPLVRKDFPDLVALLAQIPDLDLTMTTNG
                     SLLPRFAEQLKKSGLNRVTVSLDSLDNKIFKSMNDVDFPVEKVLEGMDAAKAAGLEPI
                     KVNMVVKRGVNEDSILPMARFFREKGFILRFIEYMDVGSTNGWRMNDVVSAKEIISII
                     NTEMPLEPADPNYRGEVAERWRYKDGQGEIGVIASVTQAFCRDCNRARLSAEGQLYTC
                     LFAVKGHDFRDMLRNGASDEEILHKIETVWGKRTDRYSEIRSDNTIPLPKVEMSHIGG
                     "
BASE COUNT          806 a          776 c          740 g          805 t
ORIGIN      
        1 gtccgcatgc atcgcccggt gactcacctc accaaaattt acatcgaacc caccgtcgct
       61 tgtaacctcg actgcatcac ctgcttccgc aatgcctggg atcaacccat cggcaaaatg
      121 accgatgaaa cctttgagag catcttcaac agcctcaaac aaatggaccc gattcccagc
      181 gtctattttg gcggcattgg cgaaccgctc tttcatccca aaactatcga atggatccgc
      241 cgcatcaagc aggaactcgg cgtaaaagta gaactcatca ccaacggcac caccctcacc
      301 gaacgcaaga ctcgtgagtt gatcgatgcc ggtctggata ttctctgggt gtctctagat
      361 ggcgccaccc ctgaaagctt ctcagatgta cgcatgggcg ctgaactccc cgtcattcta
      421 gaaaacctgc gccgactttt tagaatgcgt aatggcgggc acttcccaca gccagaaatt
      481 ggcgtcgcct ttgtggcgat gaagcgtaac atcaatgacc tgcccaaggt cataaaactt
      541 gggcatacct tcggtgcccg ttacttctcg gtcagcaaca ttcaacccgc cacagagggc
      601 atgcaagaag atcgtctcta taaacgcacc atccgaaaca ttgcttactt gccttcgccc
      661 gtcttgccca aactcagtct gcccaaaatg gacttcaacg aagaaaccca agccgcttta
      721 acagaagcct tcaatagcgg atgcaacgtc agcttcgcgg gcaacaactg gggcggcgca
      781 aatgacgtct gcaattttgt tgaaagcggt accatgtcca tcgcatggac gggggaagtc
      841 agcccgtgtt ggcccttgat gcacacgcac atgagctatt tgcatggcaa accgcgcctt
      901 tcaaagaaac atgttatcgg cgatactcgc aaacaatcac tggaagagat ctggctcagc
      961 ccagaatatg tcgcatatcg cgaacgcctg cacaactttg tattcgcacc ctgtaccttt
     1021 tgcggcggct gtgacctctc tgaaaccaac gaagaggatt gcctcggcaa tgatatcgcc
     1081 ccggtctgcg gcgggtgcct ctgggcgcag ggcattattc aatgcccata atttgatcta
     1141 gggttttcaa agagctcggg tcttccgagt tctttgtttt aacacgaacg aaaatgataa
     1201 aatcagaaca tgcagctacg gttttatgcc aatatgcgaa ccacagttgg aatgccactg
     1261 ctcgaaatca acgaagcgat ggtcgattca tttcgcaagt tgataccgct actcctcgaa
     1321 cagtatcccg aaactgcatt ccatttactg gatgaacatg gcgaattacg cccggatgtt
     1381 cccgtctatg tggatggaag aaacccgcgt ctttcctcgg ttggaatgga tactcctctc
     1441 aaaccggatt ctgtcatcag ctttttctcc cccatctcaa gcggaaaact aaatgtagaa
     1501 gtgttacgtg agccgactat tgacacaagg agtgaaccat gaaagtaaat ttctttgcga
     1561 ccttacgtga tattgctggt ggtaaaactg ttgagtttga ccttgaccat ggcattactg
     1621 cccgtgaatt gttggatgcc atttgtgaac gcttcccagc aatgaaaaag gaattactca
     1681 aggaagatgg cgagatgcat gggcatgtcc acttctttat taatgggcgg gatgtacaat
     1741 ttactgacct gcaatttgcc acgccgatca cacaaggcga tactgtgaat gtcttccccg
     1801 cagtgggcgg cggttaattc aggcaaattt cgaaattgaa aaagcttaga agagctatat
     1861 cagacttgag attaagaaag atgcggtttt taggtcaaaa acaacatatc cttacgggta
     1921 taatccttta tgtcagagct tgttgacact cacctactat gacaattctt gataccctca
     1981 accgtcctct tcgtgacttg cgcatctcgg tcactgatcg ctgtaatttt cgctgtgtat
     2041 actgcatgcc gaaggaaatc tttggctcag attataagtt tctgcatcac agccaggtgc
     2101 tgactttcga agagattacg cgcattgcac gtttgtttgc tggcttgggg gtcaagaaga
     2161 ttcgccttac cggaggcgaa ccccttgtac gtaaagattt tcctgacctt gtggcattac
     2221 tggcacagat ccccgacctt gatctcacga tgacaaccaa cgggtcgctt ctgcctcggt
     2281 ttgccgagca actcaaaaag tctgggttga atcgggtaac ggtcagcctt gattctttgg
     2341 ataacaagat attcaagtcg atgaacgatg tggacttccc agtggagaaa gttcttgaag
     2401 gcatggatgc cgctaaagcc gctgggttgg agccgatcaa ggttaatatg gtggtgaagc
     2461 gcggtgtgaa tgaagatagc attttgccca tggcgcgctt cttccgtgaa aaaggtttta
     2521 ttctgcgttt cattgaatac atggatgtgg ggagtaccaa tggctggcgt atgaacgatg
     2581 tggtttcggc aaaagagatc attagcatca tcaatactga aatgccacta gagcctgctg
     2641 acccgaatta ccgcggagaa gtggcagaac gttggcgtta taaagatggg cagggtgaaa
     2701 ttggcgtgat tgcctcggtg acacaagcct tttgccgtga ttgcaatcgt gcgcgccttt
     2761 ctgcagaagg gcaactttat acctgtctct tcgcagtcaa agggcatgac ttccgcgata
     2821 tgcttcggaa tggtgccagt gacgaagaga tcttgcataa gatcgaaact gtttggggta
     2881 aacgcacgga ccgatattct gaaattcgtt ctgataacac cattccgctt ccaaaagtag
     2941 aaatgtctca tattggcggt taggcatttc cgttctctat tgttcctaac ataaaaaaga
     3001 cctcccgtaa aatacgggag gtctttttta ttcaacttgt gaactagatg atttccagtt
     3061 ctttgagttt ggattcgggt acaacgccgt tcacccatcc gcgcttctcg tagtattcca
     3121 cgagcat
//
LOCUS       DAOQFT010000291         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_18923_length_3070_cov_5.477944, whole genome shotgun sequence.
ACCESSION   DAOQFT010000291 DAOQFT010000000
VERSION     DAOQFT010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18923_length_3070_cov_5.477944"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..766)
                     /locus_tag="PLR93_08135"
     CDS             complement(92..766)
                     /locus_tag="PLR93_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH26972.1"
                     /translation="MEIIKIKLSVSNAYLVKDRKSILVDTGSSHEADKILAEVKRAGV
                     NEKDISLILHTHGHVDHAGSTAELKRRLGVPVAVHAEDAFMLKTGTNGTVNPINFSAA
                     IYKQLLVKPFEPSQPDLEIDKEMTLKEFGVNARVVFTPGHTKGSLSILLGNKEAIIGD
                     VMMGGFMGGNLFPARPMVHYFFENFSEVRSSIKKVIEYKPSKLYVGHGGPLSLDAVIS
                     RFSNQI"
     gene            complement(768..1619)
                     /locus_tag="PLR93_08140"
     CDS             complement(768..1619)
                     /locus_tag="PLR93_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH26973.1"
                     /translation="MSLAKKWMFNHPVTHRVKDGYFENDGIRQHYVTLGEGKLVVLIH
                     GIPEFWYSWRHQIDALSQQYQVVAIDQRGFNESGKPASTDGYRVERLVEDVAALIRYL
                     GRKQAVIVGHDAGAWLAWHFAALHPELTERLVILSVPHPNAMREELAEHGEQHRASEY
                     ARTMQKEGAKPMFRTWQISLLLDRLAFPLYLSADSGTDADAITSFYQFNYPQEPYTLD
                     ESLPMIEAPTLVIHGKRDRFLIASGHEHNARWLKHEPKTVMLDADHFVHQEKPDEVNR
                     IILDWLA"
     gene            complement(1616..2056)
                     /locus_tag="PLR93_08145"
     CDS             complement(1616..2056)
                     /locus_tag="PLR93_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26974.1"
                     /translation="MYTVSITRLRVRSIFFMPLFTYHAMRTSMQVQKSEGIVGADTRF
                     EKNNVVWTKTVWTEESAMKKYRGSGAHQIAMRILSEMCSEASVTRWQQESTELPTWEE
                     AHRRMLTEGKQSKVKHPSALQASGKTAPDTMQSGFKMPTPPVTQ"
     gene            complement(2108..2797)
                     /locus_tag="PLR93_08150"
     CDS             complement(2108..2797)
                     /locus_tag="PLR93_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH26975.1"
                     /translation="MVRPSKKQQLPNLQEAIKETAWKQIAEKGAAALSLRAIARELKI
                     TAPAIYNYFPDRDALVTALIIDAYTSFGDSQIAARDAISKEKIEERFTAVGIAYRQWA
                     KTYPQRYQLIFGTPIPGYQAPLMEILPSAARSLSASVSVIDDLRIANRLNSSGYPNVQ
                     PGYEQQFEVWKKFAGDYDILSLAVSMLVWSRVHGLVSLEIANNMPPFGVDGDSLYMYE
                     LKSIIKQFIKP"
BASE COUNT          779 a          805 c          664 g          822 t
ORIGIN      
        1 attcgccacg cttgaccaac tccatcattg cggcgcgacc gatcgcaccc gttcctaata
       61 cgacatgatt tactgacatg agatttatcc tttagatttg atttgaaaaa cgactaataa
      121 cggcatccag acttaaaggt ccgccatgcc cgacatataa cttcgatggt ttgtattcaa
      181 tgaccttctt gatactcgat ctaacttcgc taaaattctc aaagaaatag tgaaccattg
      241 ggcgggcagg gaaaagattg ccacccataa aacctcccat catcacatcg ccaataatgg
      301 cttccttatt tcctaagagg atcgagagcg aacctttagt gtgacctggc gtaaaaacta
      361 ctctcgcatt cacaccgaat tccttcaacg tcatttcttt gtcaatttcg agatcgggct
      421 gactgggctc aaatggttta accaataatt gtttgtagat ggctgcgcta aaattaattg
      481 gattgactgt tccgtttgta ccagtcttca acatgaacgc atcctctgca tggacagcaa
      541 ccggcacgcc cagcctgcgc ttgagttccg ccgtcgaacc ggcatgatct acatgcccat
      601 gggtatgcaa aataagggaa atatcttttt cgttcacacc tgcacgctta acttcagcaa
      661 gtatcttatc tgcttcatgg ctggaaccag tatcgacaag gatcgatttt ctatccttaa
      721 ccagataagc atttgacaca ctaagcttta tcttgatgat ttccatgtta tgcaagccaa
      781 tccaaaatga ttctattgac ctcatcaggc ttttcctgat gcacaaaatg atctgcatcc
      841 aacatcacgg tctttggttc gtgcttgagc cagcgtgcat tatgttcgtg ccctgaagcg
      901 atcaagaaac ggtcacgttt cccgtgaatg accaaggtcg gtgcttcgat catcggcagg
      961 ctttcatcca gcgtatacgg ctcttgcgga taattgaatt gatagaacga agtaatcgcg
     1021 tcggcatccg ttccactatc agcagacaaa tacaggggga acgcgagccg gtctaacaac
     1081 aaactgatct gccaggtgcg aaacatcggc tttgcacctt ccttttgcat tgtgcgggca
     1141 tattcactgg cacggtgctg ctcaccatgc tccgccaatt cttcacgcat cgcattcgga
     1201 tggggaacgc tcaagatgac aagccgctcc gtcagttcgg gatgaagcgc tgcgaaatgc
     1261 catgccagcc acgcccccgc atcatgacca acgatcaccg cctgcttccg tccgagatac
     1321 cgaatcaacg ctgcaacatc ttccaccaac ctctccaccc gatagccgtc tgtggaagca
     1381 ggtttacccg actcgttgaa tccgcgttgg tcgattgcga caacctgata ctgctgcgac
     1441 aacgcatcta tctgatgccg ccatgaatac caaaactcag ggatgccgtg aatgagcacc
     1501 accaacttgc cttcacctaa tgtgacataa tgctgacgaa taccatcgtt ctcaaagtat
     1561 ccgtccttca cgcgatgagt gacagggtga ttgaacatcc atttttttgc aagactcatt
     1621 gggtcaccgg cggagtaggc attttgaaac ccgattgcat ggtgtctgga gcggtcttcc
     1681 cggaagcctg caacgctgac ggatgtttca ccttagactg tttaccctcg gtcaacatgc
     1741 ggcgatgcgc ttcttcccat gtgggcagtt ccgtgctttc ttgctgccag cgggtcactg
     1801 aagcttcaga acacatttca gacagaatac gcatggcgat ctgatgcgcg cctgaaccac
     1861 gatatttttt catcgctgat tcttccgtcc agacggtctt tgtccaaaca acgttatttt
     1921 tttcaaagcg agtatctgcg ccgacaatcc cttcagattt ttgtacctgc atgctggtac
     1981 gcatggcatg atatgtaaat agtggcataa aaaagatcga gcggactcgc aagcgggtaa
     2041 tggaaactgt atacatgatg actcctatat ttatttggtt gtgtaggtaa ggcgatattt
     2101 ttgaatatta cggtttgata aattgcttaa tgatggattt cagttcgtac atgtaaagcg
     2161 agtctccatc cacaccaaag ggcggcatgt tgttcgcgat ctccaaagaa accaagccgt
     2221 gaacacgact ccacaccaac atagagacag ccaacgacaa aatgtcgtaa tcccctgcaa
     2281 attttttcca cacttcaaat tgctgctcat agccgggctg aacatttgga tatccgctcg
     2341 aatttaatct attggctata cgcaaatcat caatgacgct cacagaggcg ctcaaagaac
     2401 gcgctgcgga cggtaggatc tccatcaatg gagcttgata tcccggaata ggcgtgccaa
     2461 aaataagctg ataccgctgc gggtatgtct tcgcccactg acggtaggca atgccaaccg
     2521 cagtaaatcg ctcttctatc ttttcttttg agatggcatc tctagcagca atctgagagt
     2581 cgccaaagga agtgtaggca tcaataatga gtgccgtcac cagcgcatcc cgatctggga
     2641 agtagttgta gatagccggc gctgtgatct tcagttcgcg tgcaatcgcc cgtagggata
     2701 atgccgccgc ccccttttca gcaatctgct tccacgcagt ttctttgatg gcttcttgaa
     2761 ggtttgggag ttgttgcttc ttgctgggtc tgaccatttc ggctccttat ttactataca
     2821 gtgtaaatat acagtgtata gtaaattaag tcaagggtaa aaaaagacct cggaggcttt
     2881 gaaaacctcc gaggtttgct atatcgcttc ttttactgcc acccattctc cggtttgttt
     2941 gggatgctcc gctagaacca aaccgcccgc gtccaccatt ttgctcaccg tcctcttcac
     3001 catctcattc ccccaaccaa acaacttttg cacatcgcgg atttgcgctg ccccgaccat
     3061 ctcaaagtat
//
LOCUS       DAOQFT010000292         3061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19032_length_3061_cov_6.926480, whole genome shotgun sequence.
ACCESSION   DAOQFT010000292 DAOQFT010000000
VERSION     DAOQFT010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19032_length_3061_cov_6.926480"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLR93_08155"
     CDS             <1..534
                     /locus_tag="PLR93_08155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNH26976.1"
                     /translation="RGRKQAQALAERWKREKMKFDFVISSPLSRARDTAEIIAAKLGL
                     TVDFEPLWLERDNGEFAGLTQHEVRNNFSHPDFTTPYDAVGNNGEGDWELFLRAGQAL
                     HDLLKREPARYLIVSHGGLLNQVMHAVVGIVPQANNAGTRFRFSNTAFAQLLYFPHQH
                     RWAIDKLNDHIHWLDAE"
     gene            622..1704
                     /locus_tag="PLR93_08160"
     CDS             622..1704
                     /locus_tag="PLR93_08160"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNH26977.1"
                     /translation="MSLPSSPSSELKFLVFGAGAIGTYIGGSLVLAGHQVVFVEKPKM
                     VEELKAKGLRLDLTVDERRNTKDVSIIDSKSFIIEPSLEDALKYGPFDAALFALKSFD
                     TVSAMEGLKPFADKLPPLLCLSNGVDNEPIIARTLGPDKVIYGTVTTAIGRRGPGSIV
                     LEKLRGVGIAAGHPLSEKLVAAFDNAYLKCRLYPDALSMKWSKMLTNLVANPTSAILN
                     MTAAEVFADKRLYKIEINMLKECLEVMASMNLEVTDLPGTPVRALAFATKLPLWLSKP
                     FLSKAAGSGRGGKMPSFHIDLYSGRPQSEVEFLHGAVVREGKTRGIPTPVNKVLTETL
                     LALTNKEILLEEFAGKPEKLLSKLRY"
     gene            1753..>3061
                     /locus_tag="PLR93_08165"
     CDS             1753..>3061
                     /locus_tag="PLR93_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNH26978.1"
                     /translation="MIQITEPTPNVEVHLPNGKTLTGPRGTKVGEFLAQVRDDFPAPI
                     VAAIINNQIHELTYPIMVESACIPVTMDTADGARIYRRSLIFLLEAAFSKTFPQGYLN
                     IDHSVSSGGYYCEVNGRDALSQQELEGLSQTMQALVKDDLPFERREVPIQEAIQYFTQ
                     HGHEDKLRLLKHRHKEYLTMYSVNGLMDYMHGYMVPSTGHLKWFGLKKVNGGFILQYP
                     RRHAPTQLAPFGDYPKLIRAFRQYGDWLETLHIDSVGVLNDAIQAGRADEIVLVSEAL
                     HEQGIADTAQQIAEKNSRIILIAGPSSSGKTTSSRRLAVQLLARGISPYPLELDNYFV
                     DRDQTPRDENGDHDFEALEALNLKRLAGDIEKLISGEEVQLPRYNFKTGRSEEGDVIR
                     LRDGQPLILEGIHGLNPRLIPDRWADSAFRLYVSALTQLNLDRH"
BASE COUNT          775 a          759 c          732 g          795 t
ORIGIN      
        1 cgcggacgga agcaagcgca agccttagcc gagcgttgga agcgcgaaaa gatgaaattt
       61 gatttcgtca tctccagtcc gctttcgaga gcgcgcgata cagcagagat catcgctgcc
      121 aaactcgggc tgaccgttga ttttgagccg ctatggttag agcgcgataa cggcgaattt
      181 gccgggctga cacagcatga agtgcgcaat aacttttctc atcctgattt caccacgcct
      241 tacgatgcag tggggaataa tggagaaggt gactgggaac tcttcctacg agcgggacag
      301 gcgttgcatg atttattgaa gcgcgagccg gcgcgttatc taatcgtctc tcatggtggg
      361 ttgttgaatc aggtcatgca tgctgtggtg gggatcgtcc ctcaagccaa caatgcgggg
      421 acgcgctttc gctttagcaa taccgctttt gcccaactgt tatattttcc acaccaacat
      481 cgctgggcaa ttgataaact caatgaccac atccattggc ttgacgccga atagtttgta
      541 actggtaaag agtgaatgcg atttgcatat cacgttcact cttatctatt tatcctttaa
      601 cacttggaat caaaaataac catgtctctc ccttcttctc catcttctga acttaaattt
      661 ctcgtctttg gcgcaggcgc cattggtacg tatatcggtg gttcgcttgt tttggcggga
      721 catcaagttg ttttcgtaga aaagccaaag atggtggaag aattaaaagc caaaggtctg
      781 cggctggacc taaccgtgga cgaaaggcgg aatacgaaag atgtaagtat tattgattca
      841 aagtctttca tcatcgagcc ttcgttggaa gatgctttga aatacggtcc tttcgatgcg
      901 gcgctctttg cattgaaatc atttgatacc gtttctgcca tggaaggctt gaagcccttc
      961 gcagataaac tcccgccttt gctttgtctc tccaatggtg tggataacga gcctatcatt
     1021 gcccgtactc tcggacccga taaagttatt tacggaacgg tcacaactgc cattggcagg
     1081 cgtggacctg gcagcattgt ccttgagaaa ttgcgtggcg tgggcatcgc agcgggacac
     1141 cccctctcag aaaagcttgt tgctgcgttt gataatgcgt atcttaagtg ccgcttgtat
     1201 cctgatgcat taagcatgaa atggtcgaag atgctgacca atttggtggc gaatcccaca
     1261 tctgccattc tcaacatgac ggcagcagaa gtctttgccg ataagcgcct gtacaagatt
     1321 gagatcaata tgctcaagga atgtttggag gtgatggcat ccatgaacct cgaagtgact
     1381 gatttgcccg gaacacccgt tcgagctttg gcatttgcaa ccaaacttcc gctttggctc
     1441 tccaaacctt tcctatccaa agccgcaggc agcggacgcg gcggtaagat gccatctttc
     1501 cacattgacc tgtattcagg tcgcccgcaa agcgaagtgg aatttttaca tggagccgtc
     1561 gtccgcgaag ggaagacacg tggaattcca actcctgtca ataaagtact cactgaaacc
     1621 ttgctcgcac tcacaaataa agaaattctg ctcgaagaat ttgcaggcaa accagagaaa
     1681 ttgttatcaa aattaagata ctaggaactt gtcaaaggtc atcgcaagca gacctttgac
     1741 atttgatctt ccatgattca aatcaccgaa ccaactccaa acgtcgaagt ccatttgcct
     1801 aatggcaaaa ccttaaccgg tccacgaggg acgaaagtgg gggagttcct tgcacaggtc
     1861 cgcgatgatt tccctgcgcc gattgtggct gccatcatca ataaccagat tcatgaattg
     1921 acctatccca tcatggtcga atcagcttgt attcctgtca ctatggatac agccgatggt
     1981 gcccgcattt accggcgttc attaatcttc cttttggaag cagctttctc taaaacattt
     2041 ccgcaaggat atttgaacat cgaccattcg gtctcctctg gcggatacta ttgtgaagtc
     2101 aacgggcgtg atgcgctttc acagcaagaa ttggaaggtc tttcgcaaac catgcaagcc
     2161 ttggttaaag atgaccttcc atttgagcgt cgcgaagttc ccattcaaga agccatccaa
     2221 tactttacac agcatgggca tgaagacaag ttgcgcttgc ttaagcatcg ccataaagaa
     2281 tatctcacca tgtactctgt taacgggctc atggattaca tgcatggata catggttcct
     2341 tctactgggc atctcaaatg gttcgggctc aagaaggtca acggcgggtt tatcttgcaa
     2401 tatccgcgcc gccatgcacc gacacagctt gccccctttg gcgattatcc gaaattgatc
     2461 cgtgcctttc gtcaatatgg cgattggctt gaaactctgc acattgacag tgttggcgtt
     2521 ttgaacgatg ccattcaagc cgggcgtgcc gatgaaattg tgctggtctc agaagcgtta
     2581 cacgaacaag gtattgcgga tacggctcaa cagatcgcag agaaaaattc gcgcattatt
     2641 ttgattgcgg gtccatcctc ttctggcaag accacatctt cgcgccgcct tgcggttcag
     2701 ttgctggcgc gcggcatctc accgtacccg cttgagttgg ataattattt tgtagaccgt
     2761 gatcaaactc cacgtgacga aaatggcgac catgattttg aggcgctcga agcactcaat
     2821 ttgaaacgcc tcgcaggcga cattgaaaaa cttatcagcg gcgaagaagt tcaattgcca
     2881 agatacaact tcaagaccgg gcgaagcgaa gaaggggatg tgatccggct gcgagacgga
     2941 caaccgctca tccttgaagg catccatgga ttgaaccccc gcctcattcc tgaccgttgg
     3001 gctgattcag cgtttcggct gtacgtctct gccctgaccc aacttaacct agaccgccat
     3061 a
//
LOCUS       DAOQFT010000293         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19072_length_3056_cov_7.664445, whole genome shotgun sequence.
ACCESSION   DAOQFT010000293 DAOQFT010000000
VERSION     DAOQFT010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19072_length_3056_cov_7.664445"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLR93_08170"
     CDS             complement(<1..1253)
                     /locus_tag="PLR93_08170"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH26979.1"
                     /translation="MTSTALETTNSIETPKDMKTFFTIWVGQLISMLGSGLTNFALAV
                     WIYDQTKQATPFAITVLLGTIPRVLLLPIAGSVADRFNRRFIMIAADTCNALLTLVIF
                     LLLGAGNLQLWHIYVIAFLESVFAAFQEPAFSASISSIVPKEKLGSANGMLQMREALS
                     TVITPVLAGILFVAIGFNGIIMIDFVTFFFAVGALLLVKIPQPEQTQEHKDKNVWQDI
                     VFGWNYLRERTGLFGLILYYAMVNFLLNWSAVLLIPMVLSRFTADVLGVIQTVMGVGM
                     LVGSVIMSAWGGAKQRIPAAIGFIVLGVTGFIIAGLQPNPWLIGAGIFLLMLSVPLAS
                     GNSQVVFQTKVPREIQGRVFSVRSTISQSMMPLAFILAGPLADKFFEPLLMDGGALAN
                     TLVGQILGTGVGRGIGLMFIISGLIG"
     gene            complement(1250..1882)
                     /locus_tag="PLR93_08175"
     CDS             complement(1250..1882)
                     /locus_tag="PLR93_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH26980.1"
                     /translation="MKKDKEEKKRFHVSDLETLRAIADPLRVQILELLEGQALTVKQV
                     AEKLGLASSKLYYHFGALEKLGLIEVAETRMVANMLEKTYRSAAHLLDVDPALFNFSK
                     GGDNESINILLSSTIDATREDLLRSLQARQFQLEQGAEEQPRRIIINRVVSSASEKRI
                     AEFQERLVKLIQEFEEEDKTSKSSDQPYALTVAFYPSFYFDKTVKKGKKK"
     gene            2032..2778
                     /locus_tag="PLR93_08180"
     CDS             2032..2778
                     /locus_tag="PLR93_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNH26981.1"
                     /translation="MQALGIDIGGSGIKGAPVNIETGELLAERLRIKTPKGAKPEPVA
                     EVVAEIVRAFKWNGPIGVGFPAPIKNGVALMAANVSEKWVGTNADELLTKTTGCVCKV
                     GNDADVAGMAEMKFGAGKGQHGTVIMITLGTGIGTAIFYNGHLLPNTEFGHLQMDGKD
                     AEHRASDAARQAKDMSWKKYAKRLNEYLNEMEKLFWPDLFIVGGGISKYHEKYLPLLE
                     LQAKIVPAQFQNEAGIVGAALYSQGMDVKS"
     gene            complement(2826..>3056)
                     /locus_tag="PLR93_08185"
     CDS             complement(2826..>3056)
                     /locus_tag="PLR93_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNH26982.1"
                     /translation="MVRLKVNKGKAHNIRPNDIVGQIAFHANIPGNIIGKIRIEEKIS
                     FVDIPDDVVEQVLKHSGNYKIGKDKFSVVKAN"
BASE COUNT          878 a          718 c          700 g          760 t
ORIGIN      
        1 ccgatcaagc cggagataat gaacatcaaa ccaatgccgc gcccgacacc cgtcccaaga
       61 atctgaccca caagggtatt tgcaagcgca ccaccgtcca tgagtaaagg ctcaaaaaat
      121 ttatcggcaa gtggacccgc cagaataaat gcgagcggca tcatggattg tgaaatagtc
      181 gagcgaacag agaagacacg cccttgaatt tcacgtggca ctttggtttg aaaaacaacc
      241 tggctattgc cgctcgcaag cggcaccgat agcatcaaga ggaagatacc agcaccaatc
      301 aaccaagggt tgggttgtaa accagcaatg ataaaaccag tcacaccgag aacgataaaa
      361 ccgatggcag ccggaatgcg ctgtttcgcg ccgccccatg cgctcataat aacgcttcct
      421 actaacatgc ccacgcccat cacagtttgg atcacaccca atacatcggc agtgaagcgg
      481 ctcaagacca tcgggatgag taaaactgca gaccagttaa gcagaaagtt gaccattgca
      541 taataaagga tcaacccaaa caggcccgtt cgttctcgca gataattcca accgaagacg
      601 atatcttgcc aaacgttctt gtctttatgt tcctgcgttt gttctggttg tggaattttc
      661 accagcaaca acgcacccac tgcaaagaag aaggtcacaa agtcgatcat gatgatgcca
      721 ttgaaaccaa tggcaacaaa taagatacca gccaacacgg gggtgataac cgtgctcaac
      781 gcttcacgca tttgaagcat gccattggca ctgcccagtt tttctttggg aacaatactg
      841 gagatcgatg cagaaaatgc tggttcttga aaagcagcaa acaccgactc taaaaaggca
      901 attacataaa tatgccaaag ttgcaaattc ccggcgccta aaagcaagaa gatcaccaag
      961 gtcaataaag cgttgcaggt gtccgcagcg atcataataa agcgtcgatt gaagcggtct
     1021 gcgacagaac ctgcgatagg cagcaataaa acacgcggaa tggttcccaa taacacggta
     1081 atcgcaaagg gagttgcttg cttagtctgg tcgtagatcc atactgcgag ggcgaagttc
     1141 gtcagcccag agccgagcat ggagattaat tgtccgaccc agatcgtgaa aaaggtcttc
     1201 atatcttttg gcgtttcaat actattcgtt gtttctaaag cggtagatgt catttctttt
     1261 tgcctttctt gacggtcttg tcaaaataaa aactcgggta gaacgctacc gttaatgcat
     1321 aaggttggtc ggaagatttt gacgttttat cttcctcttc gaattcttgg atcagtttca
     1381 ccaaacgctc ttgaaattca gcaatccgct tttcggaagc gctgctcact acgcgattga
     1441 taatgatgcg gcgcggctgt tcttctgcgc cctgctccag ttggaattgc cgggcttgta
     1501 aacttcgcag tagatcttcg cgggttgcat caatggtgga tgaaagcaaa atattaatcg
     1561 attcattatc gccacccttt gaaaaattaa agagagctgg gtctacatcc aaaagatgag
     1621 cagcagaccg ataggttttc tccaacatat tggcaaccat gcgagtctct gccacttcga
     1681 tcaaccctag tttttcaagc gcgccaaagt gatagtagag cttactggat gcaagaccca
     1741 atttctctgc tacctgctta accgttagcg cctgcccttc gagcagctcg aggatttgca
     1801 cccgcaaagg gtctgcaatg gcacgcaggg tttccaagtc ggaaacatga aagcgttttt
     1861 tctcttcttt atcttttttc atagaaaaat ttcctcttat cgttaaaata ttttttaacg
     1921 ataagtctga ttttacttat tttcgggaaa gagtcaaggg ttttccccct cccccttttt
     1981 agggaagttg gcattatcat agccctcata cctcaactta ggagtcgacg catgcaagca
     2041 ttaggcattg acattggtgg ttctggcatc aaaggcgcac ccgtcaatat tgaaacagga
     2101 gaattgctcg cagaacgttt gcgcatcaaa acgcccaaag gcgcaaagcc cgaacctgta
     2161 gcagaagtcg tagcagagat cgttcgtgca ttcaagtgga acggaccgat tggcgtgggg
     2221 ttccccgccc ccatcaaaaa cggagtcgca cttatggcgg caaacgtttc agaaaaatgg
     2281 gtaggcacga atgctgatga attgctgaca aagaccaccg gttgcgtttg taaagtaggc
     2341 aacgatgccg atgtggcagg catggcagag atgaaatttg gtgcaggcaa ggggcaacat
     2401 ggcacagtga tcatgatcac actgggcaca ggcattggta ctgctatttt ttataatggg
     2461 catttactgc ccaatacgga gtttggtcac ctgcaaatgg acggcaagga tgcagaacac
     2521 cgcgcatcag atgcggcacg tcaagccaag gatatgtctt ggaaaaaata cgccaaacgc
     2581 ctgaatgaat atctgaacga gatggaaaaa cttttctggc ccgacctgtt cattgttggc
     2641 ggcggtatca gcaaatatca cgaaaaatat ttgccacttt tggaattaca agccaagatc
     2701 gttccggcac aatttcaaaa tgaagcaggt atcgtcggtg cagcgttgta ctcgcaaggt
     2761 atggatgtaa agagttagac ttcaaaacaa aagagacctg acaaaaattg tcaggtctct
     2821 tttgtttaat ttgcttttac aacgctaaat ttgtctttgc cgattttata gttaccgctg
     2881 tgctttagca cctgctccac tacatcatca ggaatatcta caaatgagat tttttcttca
     2941 atacgaatct taccgatgat attgccgggg atattggcat ggaaggcgat ctgacccaca
     3001 atgtcgttcg ggcgaatatt gtgtgccttg cctttgttga ccttcaacct caccat
//
LOCUS       DAOQFT010000294         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19372_length_3027_cov_7.150067, whole genome shotgun sequence.
ACCESSION   DAOQFT010000294 DAOQFT010000000
VERSION     DAOQFT010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19372_length_3027_cov_7.150067"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1804)
                     /locus_tag="PLR93_08190"
     CDS             complement(<1..1804)
                     /locus_tag="PLR93_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNH26983.1"
                     /translation="MIDPEKKQTPIIERKTEYKPPSNLQSWLIGRPLSTADAPHQTIG
                     KRVGLAVFASDALSSTAYATQEILVILAAAGTMAFGYVFPISIAIVVLLAIVSISYEQ
                     TIHAYPDGGGAYIVSRDNLGEFPALIAAAALLTDYILTVSVSIASGVAQLVSAFPSLY
                     NLRVPLAVLFILFIMLINLRGVKESGAAFAVPTYFFIVVILITIATGLWRLITGSLGM
                     VLNPPEIEFHGDAIATITPFLLLHAFSSGTAALTGIEAISNGITAFKEPRSKNAGVTL
                     VWMSIILSTFFLGISFITNYVHAVPSETETVISQLARTIYDGRGTMYLMVISATTVIL
                     IMAANTAFADFPRLGALIAKDGFIPRQLAYRGSRLVYSRGIVTLAVIASILMVLFQAS
                     VTRLIPLYAIGVFLSFTLSQGGMALRWWKSGRLKPGEQKVERGSTIHYEKNWVLKMFV
                     NGFGAVCTAVVMMVFAITKFKDGAYLVLVLIPMLVGVFWMIHRHYNNLAKNLSLDNFG
                     VIPPQTHRHRVIMPVSGVHQGTLAALRYARMLSDDVTAVHIVIEPDDAEKARKKWETW
                     GEGVRLVMLDSPYRLFVEPLLEYIADLAAKRQPGE"
     gene            complement(1975..>3027)
                     /locus_tag="PLR93_08195"
     CDS             complement(1975..>3027)
                     /locus_tag="PLR93_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH26984.1"
                     /translation="LNVEASRRQLEAIINSSPDPVIVTDHRNRLLLANHAATSALKQD
                     MDTTRSGMETEKVIKLRPLLALLQSTTSEKQSTEIVLPDSRTYLATASPVMVEGRQIG
                     RVCIMRDVTHFKELDTMKSEFVATVSHDLRSPLTLMRGYATMLDTVGELNEQQQGYVK
                     KIVTGVENMSRLVNNLLDLGRIEFGVGLQVENVSVLDIIERVTGALQLQALQKNIQLN
                     VELPKDMPHAVEADQALLHQAVYNLVENAIKYTPPSGRVTIRTVSQPDQLVFAIDDSG
                     IGIPAEDVPHLFEKFYRGKQREARSQPGSGLGLAIVLSIAESHRGRVWVESTVGKGST
                     FYLQIPLVQPKETRSV"
BASE COUNT          815 a          766 c          773 g          673 t
ORIGIN      
        1 tctctccagg ttgacgtttc gctgcgaggt ctgcaatgta ttcaagcaac ggctctacga
       61 acagacgata gggcgaatcc aacatcacca agcggacgcc ttcgccccag gtttcccatt
      121 tcttgcgagc cttttcagcg tcgtctggct cgatcacgat atgcacagcc gtcacatcat
      181 ctgaaagcat gcgcgcatag cgcaaagccg cgagagttcc ctgatggacg ccgctcaccg
      241 gcataatcac gcggtgacga tgggtctgcg gtgggatgac gccaaagtta tctaacgaaa
      301 gatttttcgc gaggttgttg taatggcgat gaatcatcca gaacacaccc acaagcatgg
      361 ggatcaaaac taaaaccaga taggcgccgt ctttgaattt tgtgattgca aagaccatca
      421 tcacgaccgc ggtacaaaca gcaccaaagc cattgacgaa catcttgagc acccaattct
      481 tctcgtagtg aatggttgag ccacgctcta ccttttgttc tccgggcttg agccgtccac
      541 tcttccacca gcgcaatgcc ataccacctt gagaaagggt gaatgaaagg aacacgccaa
      601 tggcatatag aggaataaga cgggtcacac ttgcttggaa caacaccatc aagatcgaag
      661 cgatcacagc cagagtcacg ataccgcggg aataaaccaa acggctgcca cggtaagcaa
      721 gctggcgcgg aataaaacca tctttcgcga ttaaggcacc caaacgtgga aaatctgcga
      781 aggctgtatt tgccgccatg atcaaaatga ccgtggtcgc agagatcacc atcaggtaca
      841 tggttccacg cccatcgtaa atggtgcgag caagctgtga aataaccgtt tctgtttcag
      901 aaggaacggc atgtacatag ttcgtaatga aggaaatgcc aaggaagaag gtgctgagga
      961 tgatggacat ccagaccaac gtcacgccgg catttttact gcgtggttcc ttgaaagcgg
     1021 tgatgccgtt cgagatggct tcaataccgg tcaacgcagc ggtaccgctg gagaaggcat
     1081 gcagcaacag aaacggcgta atggtagcaa tggcatctcc atggaactcg atctctggtg
     1141 ggttaagaac catacccagt gaaccggtaa taagacgcca taaacctgta gcaatggtga
     1201 taaggatgac aacgataaag aaataagtag ggacggcaaa cgctgcgcct gattccttta
     1261 caccgcgcaa gttgatcagc atgatgaaaa ggataaagag caccgccaac ggcacacgta
     1321 ggttgtataa gcttgggaaa gcagaaacca attgagccac acccgaagcg atggacacag
     1381 acactgtgag gatgtagtcg gtcaacagcg ctgcagctgc gatcaatgct gggaattcac
     1441 ccaagttatc gcgcgaaacg atgtaagcac cgccgccatc ggggtaagcg tggatagtct
     1501 gttcgtaaga aatggacacg atcgccaaca acacaacaat ggcgatggat atcgggaata
     1561 cgtacccgaa tgccatggtg cccgccgccg ctaaaatgac gaggatttct tgagtggcat
     1621 atgccgtaga ggaaagcgcg tcagaggcga agaccgccag accgactcgt ttcccgatgg
     1681 tctgatgcgg agcatccgct gtagaaagcg ggcgaccgat cagccaggat tgcaaattgc
     1741 ttggtggttt gtattctgtt ttacgttcaa taatcggggt ttgttttttt tctggatcta
     1801 tcataggacc tgcatacaga caaaaatgat gcccttcccg cggaaggaca aggctggatt
     1861 atagtcggat tctgtttttc agacatcacc tacaagggtc atacaaaact actagtctat
     1921 acgtcctgca acataccgtc agccttattt tgacaacagg gggatatacg cgcgctagac
     1981 gcttctggtt tctttgggtt gcacgagagg gatctgtaga tagaaggtac tgcccttgcc
     2041 aaccgtgctt tccacccata cacgtccacg gtggctttct gcgatggata acacaatggc
     2101 gagacccaag cccgagccgg gttgagagcg cgcttcacgt tgcttaccgc gataaaactt
     2161 ctcaaacaag tgcgggacat cttcagccgg aataccgatg ccagagtcat caattgcgaa
     2221 aacgagttga tcaggctgag acacggttcg gatcgtcacc cgaccggaag gcggagtgta
     2281 cttaatggcg ttttccacca agttgtaaac tgcttggtgc aaaagtgcct gatccgcttc
     2341 cacagcgtgt ggcatatctt ttggaagttc cacattaagt tgaatattct tctgaagcgc
     2401 ttgcaattgc aaagccccgg tcacgcgctc gataatatcc aacaccgaga cgttctctac
     2461 ttgcaagcca acgccgaatt cgatgcgacc taggtcaagc aagttattca ccaagcgaga
     2521 catattctct acgccagtga caatcttttt gacatagcct tgttgctgtt cattcaactc
     2581 acccaccgta tcgagcatgg tggcataacc gcgcatgagc gtcagcggcg agcggaggtc
     2641 atggcttacg gttgccacaa actccgattt catcgtatcc aattctttga agtgagtcac
     2701 atcgcgcatg atacacacac gcccgatctg acgtccctcc accatgacgg gggaagcggt
     2761 agccaaatac gtgcggctat ccggcagaac aatttccgtc gattgttttt cagacgtagt
     2821 gctctgcaat agcgcaagca acgggcggag tttgatgacc ttctcggtct ccatgccgct
     2881 gcgagtcgtg tccatatcct gcttgagtgc agaagtagct gcatggttag caagcaacaa
     2941 acgattgcga tgatccgtca cgatcacggg atctggcgaa gaattgatga tggcttccaa
     3001 ctgtcggcgt gaagcttcta cgttcaa
//
LOCUS       DAOQFT010000295         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19409_length_3024_cov_5.756147, whole genome shotgun sequence.
ACCESSION   DAOQFT010000295 DAOQFT010000000
VERSION     DAOQFT010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19409_length_3024_cov_5.756147"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="PLR93_08200"
     CDS             <1..1471
                     /locus_tag="PLR93_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNH26985.1"
                     /translation="VKDKGTKHLGEMAAAFSKGALMGARGNSGVITSQILRGFSRGVH
                     EVETLDVETFIKALAEARDTAYKGVVKPVEGTILTVIKETANAAEAARGSVKDAIELL
                     ELAVKAADEAVKKTPEMLPILKQAGVVDSGGKGLFFFMEGMLRHIYGESLETPTMQVH
                     ALSAVGLEDALEVVEEGQDYEVVVDFVPNGELDLHGFYAKLEEMGTSIQVGEGDGMYR
                     MHIHVPTENRYVPIDYIMGIGTVTKVAIENLMAQMDDIQKSKSSQIQFSTVEPGNIAV
                     VVVSPGTGISRIFASLGVAAVVTGGQTMNPSTQDILSSFENMPTDKVIILPNNKNIVM
                     AANQAKDVTVKSVQVVPTRTVPQGLAAMLSFDPSGDVEAVAAKMNKAMGNVKTGEITV
                     ATRSVEIDGVTVKDGQVIALLDGKLVASSESVEQGVFDLLEKAEASEHEIVTLFYGAD
                     LPHAEANRVADSIREKYSNLEVEVQEGGQSHYQFILSIE"
     gene            complement(1528..>1832)
                     /locus_tag="PLR93_08205"
     CDS             complement(1528..>1832)
                     /locus_tag="PLR93_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26986.1"
                     /translation="ILLDVYLLRNALWNRLSRLFKVFIEVASLSLTVIILRTPGIIGF
                     NAESLLNGPFPPEQAKVFVTIATYTFPIVMAVVIIIQSIELIKSVYKLFKSSRQSS"
     assembly_gap    1833..1932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1933..2666)
                     /locus_tag="PLR93_08210"
     CDS             complement(<1933..2666)
                     /locus_tag="PLR93_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26987.1"
                     /translation="MNLIDRYVSEVGKHLPLLNGREDIEKELKSTLEDMLEDRAQSTG
                     RTRDEALEIEVLKEYGSPQKVASTYNPHPYLIGPKLFPFFLFVLKIVLTVVVSVMLVL
                     AGVQAVTDTPFMGADFVKIIGQGLGSALSAAIAAFGNVVLVFVIIERVLPDKEIGGFN
                     DEKDWDPASLTKEPDPDSVSRFELITEIVFTFVGLAILNIYPQWLGMFFVTGEETVFI
                     SLFSEKFFQFVPWINLTLVAQILLDV"
     gene            complement(2682..3011)
                     /locus_tag="PLR93_08215"
     CDS             complement(2682..3011)
                     /locus_tag="PLR93_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNH26988.1"
                     /translation="MANTESLENVVLELRRGVIVLAALSQLGSPEYGYSLLKKLADLG
                     LEVDQGTLYPLLRRLETQGLLESVWKLEEARPRRYYVISAEGKKLLPKMKKEWAGIVS
                     VMDKMLS"
BASE COUNT          794 a          652 c          739 g          739 t
ORIGIN      
        1 agttaaggat aaaggcacga aacatcttgg ggagatggcg gcggcgtttt ccaaaggcgc
       61 attgatggga gcacgtggta actctggcgt gatcaccagc cagatcctgc gtggattttc
      121 acgcggtgtg catgaggttg aaaccttaga tgttgaaacc tttatcaagg cgttggcaga
      181 agctcgtgat acggcatata aaggggtcgt taaacccgtt gaagggacga ttctgaccgt
      241 cattaaagaa acagctaatg cggcagaagc cgctcgcggg tccgttaaag atgcgattga
      301 attgctcgag cttgctgtca aagccgcaga tgaagcggtc aagaaaacgc ctgaaatgct
      361 tcccatcctc aagcaagcgg gcgttgtgga ctcgggtggt aaaggcttgt tcttctttat
      421 ggaaggaatg ctacgccata tttatggtga atctttagag acgcccacca tgcaggtaca
      481 tgcgctttct gcggttggtt tggaagatgc gctcgaagta gtcgaagagg gacaagatta
      541 cgaagtggtg gtggacttcg taccaaacgg tgaattggac ttgcatggtt tttatgccaa
      601 gcttgaagag atgggcacct ccattcaagt gggtgagggt gatggaatgt atcgtatgca
      661 catccatgtg ccgactgaaa atcgctatgt gccaattgat tacatcatgg gcattggcac
      721 ggtgaccaaa gtcgccattg aaaacttgat ggctcaaatg gatgatattc aaaagagcaa
      781 atcttcacag attcaattta gcacagtgga gccaggcaat attgcggtgg tggttgtttc
      841 gccaggtaca ggtattagcc gcatctttgc aagtttgggt gttgcggcag tggtcaccgg
      901 cggacagacg atgaacccct ctacacagga tattctcagc tcgttcgaga atatgcccac
      961 agataaagtt attattttgc cgaacaataa gaatattgtg atggcggcaa accaagccaa
     1021 ggatgtgacg gttaagagcg tgcaggtggt tccgacccgc acggttccgc agggacttgc
     1081 ggcgatgctt tcttttgacc ccagcggtga tgtggaagcg gttgcggcga agatgaataa
     1141 ggcgatgggc aacgtcaaaa ccggcgaaat cacggttgcg actcgttctg tggaaattga
     1201 tggtgtgacc gtgaaagatg gtcaggtgat tgcgctctta gatggcaaat tggttgcctc
     1261 ttctgagtct gttgagcaag gtgtgtttga cttgcttgag aaggcagaag ccagtgagca
     1321 tgaaattgtc actttgttct acggtgccga tttaccccat gcagaagcca atcgtgtcgc
     1381 agactccatc cgcgagaaat actccaacct cgaagttgag gtgcaggagg gtggtcagtc
     1441 tcactaccag ttcattcttt cgattgaata attgggaaac caagttaagc aaagaggctg
     1501 tcacttatgt gacagcctct tttttattta actgctttga cggctcgatt tgaataactt
     1561 gtatacagat ttgatcaatt caatactttg aatgatgata acgactgcca tcacaatggg
     1621 aaaggtgtag gttgcgatcg ttacaaagac ttttgcctgc tcaggtggga aaggtccgtt
     1681 caaaagagat tcagcattga acccgatgat ccccggtgtg cgtaggatga taacagtcag
     1741 cgagaggctg gcaacttcaa taaacacttt gaacaaccga gataagcggt tccaaagggc
     1801 atttcgcaac aggtaaacat ccagcaaaat ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nntaaacatc cagcaaaatt tgagcgacca atgtaaggtt gatccacggt
     1981 acgaattgga agaacttctc tgagaacagg gagataaaga cggtctcttc tccggtaacg
     2041 aagaacatac cgagccattg cgggtagata ttcaagattg caagcccaac aaaggtgaag
     2101 acgatttccg taatcagctc gaaacggctc acagaatcag gatcaggctc cttggtgagt
     2161 gaggcggggt cccaatcctt ttcgtcattg aagccaccaa tttctttatc cggcaagacg
     2221 cgttcaataa tgacgaagac caaaactaca tttccaaacg cagcgattgc tgcagagagg
     2281 gcactgccca atccctgccc gatgattttc acaaaatcgg cgcccatgaa aggcgtatcg
     2341 gtcacagctt gaacgcctgc caacaccagc atcacactga caactactgt aaggacgatc
     2401 ttcaatacaa agagaaaaaa cggaaacagt tttggaccga tcaaatatgg atgtgggtta
     2461 taggtggatg caactttttg cggcgagccg tattctttca agacttcgat ttcgagtgct
     2521 tcatcacgag tgcgcccggt actttgagcg cggtcttcca acatatcttc cagcgtggac
     2581 tttaactctt tttcaatgtc ttcacgccca ttcaataacg gcaaatgctt gccaacttca
     2641 gaaacataac ggtcaataag attcatgatt cttccctttc tttatgagag catcttgtcc
     2701 atgacagaga caatgccagc ccattctttt ttcatcttcg gcagaagttt tttgccttct
     2761 gcactgatca cataataacg acgcggacgc gcttcttcca acttccaaac tgactcaagc
     2821 agaccctgtg tttccaagcg gcggaggagg ggatacaacg taccctgatc cacttccaag
     2881 ccgaggtctg cgagtttctt cagcagtgaa tatccgtact ctggcgagcc taattgacta
     2941 agcgccgcca atacgattac accgcgcctt aactccaaaa caacattttc gagagattct
     3001 gtgtttgcca tatctcatcc ttta
//
LOCUS       DAOQFT010000296         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19487_length_3018_cov_8.007087, whole genome shotgun sequence.
ACCESSION   DAOQFT010000296 DAOQFT010000000
VERSION     DAOQFT010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19487_length_3018_cov_8.007087"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLR93_08220"
     CDS             complement(<1..191)
                     /locus_tag="PLR93_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26989.1"
                     /translation="MLRSRTFLYFLIGISLLIALVFGGYYLYNTYRVRTVVIATNEEG
                     SQSYIMMDALAKAVQQRDSR"
     gene            complement(209..1042)
                     /locus_tag="PLR93_08225"
     CDS             complement(209..1042)
                     /locus_tag="PLR93_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH26990.1"
                     /translation="MSIVNRLLSIMHLQSLTPRPFLDSDLNEFPFTVPVIRSLPEIKF
                     TSPVTFFVGENGSGKSTVLEALACAVESITVGTESVKTDKTLAPVRKLSKYFKLAWTK
                     RTRKGFFMRAEDFFGYARSMRETREELEEEYNNVERDYEGRSKYAADLARSAYAGQLA
                     AMKNRYGRDLDNFSHGEAFLTLFQARFVPGGLYLLDEPEAALSPSRQLSFLSALRQMV
                     EEEAQFIIATHSPIILAYPGAQIFSFQNGAIKQVQYEELEHVQLTKDFLNNPQSYLRH
                     L"
     gene            complement(1073..2326)
                     /locus_tag="PLR93_08230"
     CDS             complement(1073..2326)
                     /locus_tag="PLR93_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNH26991.1"
                     /translation="MKSDLDAIMQSRNLDAFVVFGNAENNPPMYYLTGGGHVSHATVI
                     KKRGEDAVYFYNDMERDEAAKSGLKLTPYSKYDYNELLQKANGDLLLTSALRYQQMFE
                     DVGVTKGRVGVYGHYDVGAVFGWLNHLQKLMPAIEFVGEARDDSIFLRAMETKDENEV
                     ARIRKMGKVTTAVVERVKEYLTSCDVREDEVLLKEDGTPLSVGDVHSKIRLWVSEHGA
                     ELPSGFIFAIGRDAGVPHSAGNPADLMRLGQTIVFDIYPAEAGGGYFYDFTRTWSLGY
                     TTPEAQSLFDQVKEIYDTLNDNFDLNVAFKDYQRMTCEFFEGKGHQTPLNTKAPIEGY
                     VHSLGHGVGLNIHERPFSGLTAGDDQRLRPGVIITSEPGLYYPEKGMGFRIEDTLWVR
                     PDGVMEKLADYPYDFVLPMKKWKKK"
     gene            complement(2323..>3018)
                     /locus_tag="PLR93_08235"
     CDS             complement(2323..>3018)
                     /locus_tag="PLR93_08235"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNH26992.1"
                     /translation="NFKIVGFTEEPDFKDIVDAAHKAGIPVVDDLGSGALYDTAKYGF
                     SHEPTVQESMQAGADVILFSGDKLLGGPQAGIIIGKKELLDRIKKHPLARAVRADKSC
                     LAGISATLTHYLKDEAEREIPIVRMMSLTLKQVKGRAEAWREELGQGNVVEGESTVGG
                     GSLPDESIPTFLLELQVKSAEKFLRALRKNNPPIIARTENDKVLLDPRTVFPEQEGAL
                     LVGLKNLLKKENK"
BASE COUNT          789 a          809 c          661 g          759 t
ORIGIN      
        1 cgagaatcac gctgttgaac cgccttcgcc aaagcatcca tcatgatgta actctggctg
       61 ccttcttcat ttgtcgcaat aaccaccgta cgaacacggt acgtgttgta caagtaatat
      121 ccaccaaaca ccaatgcgat caataaggaa atgccgatca agaaataaag aaaagtgcgc
      181 gagcgaagca ttgagatcat tcctgtatct ataaatgccg caagtaagat tgcgggttat
      241 tcaaaaaatc cttggtaagt tggacgtgtt caagctcctc gtattgtact tgctttatag
      301 ccccattttg aaagctgaat atttgcgccc ccggatacgc caaaatgatc ggcgaatgag
      361 tagcgatgat aaactgcgcc tcttcttcga ccatttgcct gagtgccgaa aggaagctaa
      421 gctgtctgga cggcgaaagc gcagcctcag gttcatccaa cagatataag ccaccgggga
      481 caaaacgcgc ctgaaacaaa gtgaggaatg cctcgccgtg cgagaagttg tccaagtctc
      541 taccgtaacg gttcttcatc gccgcaagtt gaccggcata cgccgaacgt gccaagtcag
      601 cagcatattt cgagcgtcct tcataatcgc gctctacatt gttgtattct tcttccagtt
      661 cttcacgcgt ctcccgcatg gaccgggcat atccgaaaaa atcttcggcg cgcataaaga
      721 agcccttgcg cgtacgtttc gtccatgcaa gtttgaaata ctttgatagc ttacgaacag
      781 gcgcaagggt tttatcggtc ttgacgcttt ccgtccccac cgtaatggac tctaccgcac
      841 aagccaatgc ctctaacacc gtcgatttgc ccgagccgtt ctcccccaca aaaaaggtta
      901 ccggtgaggt gaacttgatc tctggcaaag aacgaatcac cggcaccgtg aacggaaatt
      961 cattcaggtc actatcgaga aatggacgag gtgtaagcga ttgaaggtgc atgatggaca
     1021 atagacgatt gacgatggac attctttcat ggtccatcgt caatcgtcga cattatttct
     1081 ttttccactt cttcattggc aaaacaaaat catacgggta atctgccagc ttttccatga
     1141 cgccatcagg gcgaacccaa agcgtatctt cgatacggaa gcccatccct ttttcggggt
     1201 agtacaagcc cggttcggag gtaatgatca ctccgggtct gaggcgttga tcgtcccccg
     1261 ccgtgagccc actgaatggg cgttcgtgga tgttcaaccc aacaccatgc ccgaggctgt
     1321 ggacatatcc ctcaattggc gctttggtat tgagcggagt ttgatgccct ttgccttcaa
     1381 agaattcgca ggtcatgcgt tgataatcct tgaatgccac atttaagtca aaattatcgt
     1441 tgagcgtatc gtagatttct ttgacttggt caaacaatga ttgcgcttca ggcgtagtat
     1501 agccaaggct ccatgtacgg gtgaaatcat agaaatagcc accacctgct tctgccggat
     1561 aaatatcaaa aacaatcgtc tgacccaaac gcatcaggtc agcggggttg cccgcggagt
     1621 gcggcacacc ggcgtcgcga ccaatggcaa agataaaacc cgaaggcaac tctgcgccat
     1681 gttcagaaac ccaaagccgt atcttcgagt ggacatctcc tacgctaagc ggagtgccat
     1741 cttccttgag taacacctca tcctcgcgta catcgcaaga cgtgagatat tccttcaccc
     1801 tctccacgac tgcggtagtt accttaccca ttttgcggat acgcgcgact tcattctcat
     1861 ccttggtttc catggcgcgc aaaaagatag agtcatcacg tgcttcaccg acaaactcaa
     1921 tcgcgggcat caacttctga agatggttca gccacccaaa caccgcacca acatcataat
     1981 gaccatatac accaacacgt ccctttgtca ccccaacatc ttcaaacatc tgttgatacc
     2041 gcaaagcact tgtcaacaaa agatcgccat tggctttctg taacaattcg ttatagtcat
     2101 atttgctata gggagttaac ttcaatccgc tcttggcggc ttcatcgcgc tccatatcat
     2161 tgtaaaaata aaccgcatct tcgccgcgtt tcttgatcac cgtagcatgg ctaacatgcc
     2221 caccaccggt cagataatac atgggcgggt tattctcagc attaccaaat acaacgaatg
     2281 catccaagtt gcgagattgc ataatggcat cgaggtctga tttcatttat tttccttttt
     2341 caacaaattt ttcaacccaa ctaataacgc accttcttgt tcaggaaaaa cagtacgagg
     2401 gtcgagcagc actttatcat tctctgttcg agcaataata ggcggattat tctttcgtaa
     2461 ggcgcgtaaa aatttttcgg cagatttcac ttgcaattca agcaaaaagg tcgggatgga
     2521 ttcatccgga agcgacccgc cccccaccgt agactcacct tccactacat tgccttgacc
     2581 taactcctct cgccacgcct cagcccgacc tttgacctgc ttcagcgtga gagacatcat
     2641 cctcacaatg ggaatttctc gctccgcctc gtctttgagg taatgtgtga gcgtagcaga
     2701 gatgcctgcc aaacaagatt tatctgcgcg gacggctctc gccagcggat gtttcttaat
     2761 tctatcgagc agctcctttt tgccgatgat gatgcccgcc tgtggaccgc caagcagttt
     2821 atcgccggaa aagagaatca catccgcccc agcctgcata gactcttgaa cggtcggttc
     2881 gtgagagaag ccatatttgg cggtgtcgta caaagcgccc gagccgaggt catctacgac
     2941 gggaatgcca gctttatggg cagcatcgac tatgtctttg aaatcgggtt cttcagtaaa
     3001 gcccacgatc ttgaaatt
//
LOCUS       DAOQFT010000297         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19572_length_3010_cov_6.556007, whole genome shotgun sequence.
ACCESSION   DAOQFT010000297 DAOQFT010000000
VERSION     DAOQFT010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19572_length_3010_cov_6.556007"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="PLR93_08240"
     CDS             complement(<1..1425)
                     /locus_tag="PLR93_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNH26993.1"
                     /translation="MSNEYDLKSVKLPYLAGGVLKLFASLVEGPLQSALIPSLFESAG
                     LTWLRKQQIEEAPTYLPIHYVGELQKKDAAIPEAQWPVTPSQTRGFQFASIFDFAKAY
                     RAGTTTPEDVAKKVLDAIEASDKSDAPLRAFIAVNRDEVMRQARESTERFKAGKPLSI
                     FDGVPVAIKDEMDVTPYPTTVGTSFLGKSPAKEDSEVAARLRKAGAMLVGKTNMHEIG
                     INVFGLNPHHGTTRNPYNTGHFTGGSSSGSATAVAAGLVPVAIGADGGGSIRIPAAFC
                     GLVGLKATFGRVSEYGAFPLDWSVAHIGPIASTATDVALTYALLAGPDLKDEVSLHQP
                     LPTLEGWDNINLKGLKIGIYRPWFEHAEKEVVDACTAMLKKFEEMGCEIKEIVIPNLE
                     ANRVAHTVTIVSEMVQGMSATYDQHHKEHGLDVRINLALGRKFSADDYVKAQRIRTRM
                     IRHFNNAFKEVDVILTPTTAIAAPE"
     gene            complement(1563..2105)
                     /locus_tag="PLR93_08245"
     CDS             complement(1563..2105)
                     /locus_tag="PLR93_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH26994.1"
                     /translation="MSSTIALLMFIALIGFIAFLFWRQNRIFNDIQAFYKDNKMVYQE
                     TAPVEHPFIYTSIKPICSKGSLKPDMPFTFMLGSRSEGAGQSRVLHTYIGVFIPDHPR
                     LTDAWLNTWKHKVAERGDNWAQHSDVEPHEKSWGLMGAPEDLPIVAKRINNGVLIAWS
                     GLHIRKVFEARLDELKQTLS"
     gene            complement(2129..2461)
                     /locus_tag="PLR93_08250"
     CDS             complement(2129..2461)
                     /locus_tag="PLR93_08250"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNH26995.1"
                     /translation="MAYDLKLAERIRAEVKGLPIVEKKMFGGVGYMLHGNMACGILED
                     NLIIRVALDDYEKTLKRKYVKIFTMKNGPRPMKGWVMVEPEGYKTSKQLKDWVKTGID
                     VAINLPPK"
     gene            complement(2481..>3010)
                     /gene="upp"
                     /locus_tag="PLR93_08255"
     CDS             complement(2481..>3010)
                     /gene="upp"
                     /locus_tag="PLR93_08255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNH26996.1"
                     /translation="KEIAGLLAYEATLDLATTPVEIETPLETTTAQELKEKIGLVPIL
                     RAGLGMVEGIWELMPSAEVWHIGLYRDEKTLRPVEYYNKLPLEPRVAVCLILDPMLAT
                     GGSATATAEVLKRWGVTKIKFVGLIAAPEGIKAMQTAHPDIDIYVAAIDDHLNERAYI
                     VPGLGDAGDRQFGTG"
BASE COUNT          690 a          733 c          746 g          841 t
ORIGIN      
        1 ctctggagcg gcaatggcag tggtgggggt cagaatgaca tccacttctt tgaaggcatt
       61 attaaagtga cggatcatcc ttgtacggat gcgctgtgct ttgacataat cgtctgcact
      121 gaactttcgc ccgagcgcca gattgatgcg tacatccaac ccatgttctt tgtggtgttg
      181 gtcataggtt gcgctcatcc cttgcaccat ctcactgacg atggtcactg tatgggcaac
      241 tcggtttgct tcaaggttcg ggatgacgat ctctttgatc tcacagccca tctcttcaaa
      301 ttttttgagc atggcagtac aggcatcaac gacttctttt tctgcatgtt caaaccatgg
      361 acgataaata ccgattttga gccccttgag gttgatattg tcccagccct ctaaggtggg
      421 caaaggttgg tggagagaaa cttcatcttt caggtcgggt cctgccagaa gggcataggt
      481 caatgcgaca tcggtggctg tgctagcaat gggaccaata tgcgccacgc tccaatccaa
      541 cggaaatgcg ccatattcgc tcacacgccc aaatgtagct ttcaagccga ccaaaccgca
      601 aaacgccgcc ggaatacgaa tcgacccacc tccatcggca ccgatggcga cgggcaccag
      661 tcctgcagca acagcggtgg cagagccgct tgaacttcct cctgtaaagt gaccggtgtt
      721 gtatgggttg cgggttgtgc cgtgatgagg gttcaaccca aagacattga tgccgatttc
      781 atgcatgttt gtcttgccga ccaacatggc acccgctttg cgtaaacgtg ccgccacttc
      841 cgaatcttct ttggctggag atttccctaa gaaggatgtg ccgactgtcg ttggatacgg
      901 tgttacatcc atttcatctt tgatcgccac tggcacacca tcgaagatgc tgagcggttt
      961 gccagccttg aaacgttccg tcgactcgcg ggcttgacgc ataacttcat cgcggttcac
     1021 ggcgatgaat gctcgtagcg gcgcatcaga cttgtcactg gcttcaatgg catctaagac
     1081 cttttttgcc acgtcttcgg gtgttgtcgt tccggcacga taggctttgg caaaatcgaa
     1141 aatgctcgca aattgaaagc cacgtgtttg agatggtgtt acgggccact gtgcttcggg
     1201 aattgccgcg tctttttttt gtaattcacc cacatagtgg atcggcagat aagtaggtgc
     1261 ttcttcaatc tgttgtttac gtagccaagt caacccggcg ctttcaaaca agctgggaat
     1321 caatgcactt tgcaatggtc cttccactaa tgaggcaaac aacttcaata ccccgcctgc
     1381 cagataaggc aacttcacag atttgagatc gtattcattg ctcattgttt ctcctcaagg
     1441 aaaatgctgg tgtgtggtgc aaatcactgc gggtatcact tgtcagtgtg agtatcagtt
     1501 tgggatgttg aacccgcttc aaatgagtcg gaagcggggt tgtatcccat atgatttggt
     1561 tgttaggaga gcgtttgctt taattcgtct agccgtgctt caaatacttt tcgaatatgc
     1621 agccctgacc aggcaattag aacgccattg ttgatgcgtt tggcaacaat gggtaaatct
     1681 tcaggggctc ccatcagtcc ccagcttttt tcatggggtt ccacatcgga atgttgtgcc
     1741 cagttatcac cacgttctgc tactttgtgt ttccaagtat tgagccaggc atcggtcagc
     1801 cgtgggtggt ctgggatgaa gaccccaatg taggtgtgga gaactcgaga ttgcccagca
     1861 ccttcagacc tggaaccaag catgaatgtg aaaggcatat caggctttag gctacccttt
     1921 gaacagatcg gttttatgct ggtgtaaata aaaggatgct ctacaggggc agtttcttgg
     1981 tagaccatct tattgtcttt gtaaaaagct tgaatgtcgt tgaagattct attttgacgc
     2041 caaaacaaga aggcgataaa accgatgagc gcaataaaca tcaataaagc gattgtggaa
     2101 gacattctca atctcctttt atgtatattt acttgggagg taggttaata gcaacatcta
     2161 tgcctgtttt tacccaatcc ttaagttgtt ttgatgtttt gtatccttct ggctccacca
     2221 tgacccagcc cttcatgggt ctgggtccgt ttttcatggt gaatattttg acgtatttgc
     2281 gtttgagagt cttttcataa tcatccagcg caacacgtat gattaagtta tcttcaagaa
     2341 tgccgcatgc catgttgcca tgcagcatat agccaacgcc gccaaacatc tttttctcga
     2401 cgatgggtaa gccttttact tcagcgcgaa tccgctctgc aagtttcagg tcgtatgcca
     2461 taagtgactt ggtgtatcgt ttaccctgtt ccgaactgac ggtctccagc gtcacccaag
     2521 ccgggtacga tgtacgcccg ttcgttcaga tgatcatcaa tggcggcaac atagatatca
     2581 atatcggggt gagccgtttg catggcttta atgccttctg gcgccgcgat cagccctacg
     2641 aatttgatct tggtcactcc ccaacgtttg agcacttccg ctgtggctgt ggcagaacca
     2701 ccggtggcaa gcatggggtc aagaatgagg cagaccgcta cgcgcggctc aagcgggagt
     2761 ttgttgtagt attccacagg acgtagggtt ttttcatcac ggtataaacc gatatgccaa
     2821 acttcggcac tgggcatgag ttcccaaatg ccttccacca ttcccagccc ggcacgtaga
     2881 atcggcacaa gcccgatctt ttctttgagc tcttgcgctg tagtagtttc cagcggagtt
     2941 tcgatctcaa ctggggtggt ggcgaggtcg agtgtggctt cataggcaag aagtcccgcg
     3001 atctctttca
//
LOCUS       DAOQFT010000298         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19668_length_3002_cov_5.846963, whole genome shotgun sequence.
ACCESSION   DAOQFT010000298 DAOQFT010000000
VERSION     DAOQFT010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19668_length_3002_cov_5.846963"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PLR93_08260"
     CDS             <1..1333
                     /locus_tag="PLR93_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNH26997.1"
                     /translation="RQRDNPSMVKAGRAFIAIMFFVGLIGVLVNGAPIFSRFLYSSVL
                     LALFAWGWTRLIIRSLSFERVTRELRANVGDVFEETFKLSNRSRLPIPWVEIVNKSTL
                     PFAAGSRLFTLVPGKQFRSYMARTWLTRRGRFELGPTRITVGDPFGLFATSKEHKSKD
                     ALIVFPMIHEIYAFQTPQGVLTGGQVIRKKSPDITPHAAGVREYVSGDAMKRIHWSTS
                     ARRNRLMVKEFEQDPQAEVWLYLDAHQTVHYQKRHEQQEMALHDMLLNKRPKIKLPPS
                     TLEYGVSIAASLAHYFLRQRRSVGFASAGQTFTVLPAERSERQEAKILETLAFVKADG
                     NLSLAGLVATQAAVLPQGSTVILITPTIRSDLLQAVDDLVYHYLSPVIVLLNAKSFEG
                     PSGTAELVSSLKARRVPVITISCDDDLPQALADISSNFNLQELHAWRTPLS"
     gene            1312..2214
                     /locus_tag="PLR93_08265"
     CDS             1312..2214
                     /locus_tag="PLR93_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH26998.1"
                     /translation="MANTPIVTIDLHFQGKTQAIASYLIRQGDAVVLIESGPGSTLSA
                     LEAGLAKEGLSPRDITHVLLTHIHLDHAGAAGWLSRQGAEIYVHPIGAPHLLNPEKLI
                     ASATRIYGDRMDTLWGEFLPVAQEQLKTPNDAEEIVIGTMRFLPVNTPGHAEHHYSYV
                     FEDVCFSGDVGGVRIPGFQYLRAPMPPPELHFGRWRESLARLRGMKFAHIAPTHFGMF
                     DDVQWHLSTLDETLAAMEKWLETVMAQDPSIEKLREEFTLWMEEEGKAKNLSEDVLRA
                     YSLSNPVAMSADGLMRYWKKVRSA"
     gene            2292..>3002
                     /gene="argF"
                     /locus_tag="PLR93_08270"
     CDS             2292..>3002
                     /gene="argF"
                     /locus_tag="PLR93_08270"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNH26999.1"
                     /translation="MKDFIAISDYSPEEIEDLLDLAVKLKKQYFKKGNKPIFQGKVLG
                     MIFQKPSLRTRVSFDMAMRHCGGDALYLSPNEIGLGKRESIADIARVLSGYVQALMAR
                     TFDHAHVVELAKWADIPVINGLSDYNHPCQGMADALTIIEKFGKDSKGLNVSYVGDGN
                     NVTVSLMHVAAQLGWNMTVGTPEGYDVNPQAVEIAQNIARKTGSKLTFVRDVHEAVKN
                     AHVIYTDTWTSMGQEEETA"
BASE COUNT          759 a          685 c          789 g          769 t
ORIGIN      
        1 acggcaaagg gataacccaa gcatggtgaa agccggacga gcctttattg ccattatgtt
       61 ctttgtaggg ttgatcggtg ttctggttaa tggggcaccg atcttttcac gttttttgta
      121 tagcagtgtt ttgcttgcac tctttgcctg gggttggacg cgcctcatta tccgcagttt
      181 gagctttgag cgtgtgacgc gtgaactacg tgccaatgtg ggggatgtat tcgaagaaac
      241 cttcaagcta agtaatcgca gtcggttgcc catcccatgg gtggagatcg ttaacaaatc
      301 cacgttgcca tttgcagcgg ggtcacgttt gtttaccttg gtgccgggta aacaattccg
      361 cagctacatg gcgcggactt ggctcactcg ccgtggacga tttgaattag gtccaacgcg
      421 catcacagta ggagacccat ttggattgtt cgcgacaagc aaagaacaca aatccaaaga
      481 tgccttgatc gttttcccga tgatccatga gatttacgcc ttccagactc cacaaggcgt
      541 gttaactggc ggacaggtga ttcgaaagaa atcgccagat attactccgc atgcggcggg
      601 cgtacgtgaa tatgtgagtg gcgatgccat gaagcgcatt cattggtcta caagcgcgcg
      661 acgtaaccgt ttgatggtga aagaatttga gcaagacccc caagcagaag tgtggttata
      721 tcttgatgcg caccaaacgg ttcattatca gaaacggcat gaacagcaag aaatggcgtt
      781 gcatgatatg ttgttgaaca agcgccccaa gatcaaattg ccgccttcta ctttggaata
      841 tggggtgagc attgcggcat cactagcgca ttacttcctt cgccagcgcc gttcggtagg
      901 gtttgccagc gccgggcaga cctttactgt tttgcccgcc gaacgcagtg aaagacagga
      961 agcgaaaatc ctcgaaacct tggcattcgt caaagcagat ggcaaccttt cattggctgg
     1021 gttggtggca acacaagcgg ctgtcttgcc gcaaggctca acggtgattc tcatcacccc
     1081 aaccattcgt tcagaccttt tgcaagccgt ggatgatctg gtgtatcact atctttctcc
     1141 ggtaattgtg ttattaaatg caaagtcatt tgagggtcca tctggaactg cagaactggt
     1201 atcatcactc aaagcacggc gcgtgccggt catcaccatt tcatgtgacg atgatctacc
     1261 gcaggcgctg gcagacatat cctctaattt caacctacag gaattgcatg catggcgaac
     1321 acccctatcg taacgattga cctccatttt caaggcaaga ctcaagccat cgcctcgtat
     1381 ttgatacgac aaggcgatgc tgttgtctta atcgaaagcg gacccggctc gactctttct
     1441 gcgctcgaag ctggtttggc gaaagagggc ttatctcccc gagacataac ccacgttctg
     1501 ctcacacaca ttcatcttga ccatgcaggg gcggcgggtt ggttgtctcg tcagggcgcg
     1561 gagatttatg tacaccccat tggggctccg catttattga accccgagaa actgatagcc
     1621 agtgccaccc gtatttacgg agataggatg gatacacttt ggggtgaatt tctgcccgta
     1681 gcgcaggaac agctcaaaac tccgaatgat gcggaggaga ttgtcatcgg tactatgcga
     1741 ttccttccag tcaatacacc ggggcatgca gaacatcatt attcctatgt ttttgaagat
     1801 gtctgtttta gtggggatgt aggcggggta cgtattccgg gctttcaata tttgcgtgcg
     1861 cctatgccac cacccgagtt gcattttggg cgttggcgtg aaagtttggc gcgtttgcgc
     1921 ggaatgaaat ttgcgcatat cgccccgacc cattttggca tgtttgatga tgtgcaatgg
     1981 catttgagca ccctcgatga gacgctggct gcaatggaga aatggcttga aacagtgatg
     2041 gcgcaagacc cgtccattga gaagttgcgc gaagaattta cgttgtggat ggaagaagaa
     2101 ggtaaggcaa agaacttgag cgaagacgtg ttgcgggctt attctctctc taacccggtg
     2161 gcgatgagtg ccgatgggct aatgcggtat tggaagaaag ttcgcagtgc gtaatttgtc
     2221 atattctttg cgtgatgctt gaaacatggc aaaatagact aaaaaagata tactttccct
     2281 gaggagcaca catgaaggac tttattgcga tttctgacta ttcaccagaa gaaattgaag
     2341 acctgctcga ccttgctgta aaactcaaaa aacaatattt caaaaaaggc aacaaaccga
     2401 tctttcaagg caaagtgctg ggaatgatct tccaaaaacc aagcctgcgt acgcgcgttt
     2461 cttttgatat ggcgatgcga cattgtggcg gcgatgcctt gtatctttct ccaaacgaaa
     2521 ttggtttggg caagcgcgaa tcaattgctg atattgcgcg agtgttatct ggttatgttc
     2581 aagcattgat ggcacgtacc tttgaccatg ctcacgtggt ggaactcgcc aaatgggcag
     2641 atattccggt gatcaatggc ttgagcgatt acaaccatcc ctgtcagggc atggcagatg
     2701 cattgaccat cattgaaaaa tttggcaaag actcaaaagg tttgaatgtt agttatgtcg
     2761 gcgatggcaa taacgtcacg gtttcgttga tgcatgtagc agctcaactg ggttggaaca
     2821 tgacggttgg cacgcccgaa ggctacgacg tcaacccgca ggcggtggaa attgctcaga
     2881 acatcgccag aaaaacgggc agcaagttga cttttgtccg cgatgtgcat gaagcagtga
     2941 agaatgctca tgtgatttat accgatacgt ggaccagcat gggacaggaa gaagaaaccg
     3001 cc
//
LOCUS       DAOQFT010000299         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19991_length_2971_cov_6.427641, whole genome shotgun sequence.
ACCESSION   DAOQFT010000299 DAOQFT010000000
VERSION     DAOQFT010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19991_length_2971_cov_6.427641"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLR93_08275"
     CDS             <1..474
                     /locus_tag="PLR93_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH27000.1"
                     /translation="VQIIFNIFRQRPAELFFPEAMRRKVGILARVPLASGMLTGKLSA
                     KSTFEKDDHRAFNRHGESFDRGETFSGVDYETGLQAVEELKGLIPNGWSMPQFALRWI
                     LMHDAVTCTIPGAKNPAQAEDNVKAADQPSLSPEAMEKIKAVYDSQIRSLVHNYW"
     gene            502..1476
                     /locus_tag="PLR93_08280"
     CDS             502..1476
                     /locus_tag="PLR93_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH27001.1"
                     /translation="MIYGEVEGIKKPIARVVQGTTMLGSDLNEEESFSLLDQVYELGG
                     NTLDTAHVYAGGNSERIIGKWMHSRGLRKKMTIITKGGAHSQDRRRMTSFDVTSDLFD
                     SLARLKTDYIDLYLLHRDDTNLPVEPIIHTLNEHLQAGRIHAFGASNWSHQRMQDAAT
                     YAKANGLKTFIASSPQYSLADSNAEPWPLCLSISGDTGKEAREWYANTAMPVLVWSPL
                     ASGFFSGRFRRDNLREFGEREWDEVVVRTYATEANFQRLDRAEALAKEKGCTAAQIAL
                     AYVMNQPMNVFSVVGSHSIEKFKANIEAAELKLTNQELAWLDLKVENL"
     gene            1476..2324
                     /locus_tag="PLR93_08285"
     CDS             1476..2324
                     /locus_tag="PLR93_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27002.1"
                     /translation="MSKQTLKNNFFELEYLTHSLRISSITPHGKTNLLADLSHQPPVP
                     TPYGDFYLRGGHRLWHAPEAMPRTYVPDTGEIKITNIPNGVVLETETEPGANIRKRIE
                     IKLAVDTPSLTLTHTLFNDGLWTLELAPWAITQFRLGGIAILPMPTEKVDDAALLPNR
                     QISFWTYTRLNDKRLSLRDEFSLFHAESSLPPFKMGYFNPHGWLAYYIDGVLFKKTFN
                     ASTLAPYPDNNCNAELFCNDEFVELESLGPLTKLEPGASVQHQETWEILDANALPQDI
                     QNLILK"
     gene            complement(2331..>2971)
                     /locus_tag="PLR93_08290"
     CDS             complement(2331..>2971)
                     /locus_tag="PLR93_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HNH27003.1"
                     /translation="DLSTSFLFHGLAPWEKLETLYHKVLVVIVKSRGKLGSPLRAFRG
                     IMPTLSLMIESENAFLEFLNAKGFVYSRMEHPAVFTCAEADAHHIGVDAVSTKNLFLC
                     DKKARRFFLAVTACEKTVRLDELSGLFGVPHLRFASEQNLLRLLGVAGGSVTMMGLAN
                     DTEHIVELWIDNEIWQAENFLCHPLVNTATLTLTKAELERFLALTGHTPKFF"
BASE COUNT          842 a          728 c          677 g          724 t
ORIGIN      
        1 gtgcagatca tcttcaatat tttccgccag cgcccggctg agctattctt ccctgaagcc
       61 atgcgccgca aagtgggtat tctcgcacgt gttccgctcg ccagcggaat gttaactggc
      121 aaactcagcg cgaaatctac ttttgaaaaa gatgaccacc gcgcctttaa tcgtcatggt
      181 gaatcatttg accgtggtga aaccttctca ggtgtggatt atgaaaccgg tttgcaagcg
      241 gtggaagaat tgaaagggct tatccccaat ggttggagta tgccgcaatt cgcactgcgt
      301 tggattctga tgcatgatgc tgtcacttgc acgatacccg gtgcaaagaa tccggctcaa
      361 gcggaagata atgtcaaggc ggcagaccag ccgtccctga gcccagaggc gatggaaaaa
      421 atcaaagccg tgtacgactc tcagatccgc agtctcgttc acaattattg gtaaaacaaa
      481 tccaatcgac agaggtattt gatgatctat ggcgaggttg aaggcataaa aaagccaatt
      541 gcccgtgttg tgcaaggaac aactatgttg ggcagtgatt tgaatgagga agaaagtttt
      601 tctcttttag atcaagtata tgaacttggc ggtaacacac tcgatacagc gcatgtgtac
      661 gctggtggaa acagcgaacg cattattgga aaatggatgc actcgcgcgg attgcgtaag
      721 aaaatgacca tcattaccaa aggcggcgca cattcacaag accgccgcag aatgacgtct
      781 tttgatgtta cctctgacct attcgactca cttgcacgcc tcaagacaga ttacattgac
      841 ctttacctgt tgcatcgtga tgataccaat ctgccggttg agccgattat tcacacactc
      901 aatgaacatt tacaagcggg tcgcattcat gcgtttggcg catcgaactg gtctcaccaa
      961 cggatgcaag atgctgcaac ctatgcaaaa gccaatggct tgaaaacatt catagccagc
     1021 agcccacaat acagcctagc agactcaaac gctgaacctt ggcccttatg tttgagcatt
     1081 agcggtgaca ctggcaaaga agccagagaa tggtatgcca acaccgcaat gccggttttg
     1141 gtgtggtctc cacttgccag tgggtttttc tctgggcgtt ttcgaagaga caacctgcgc
     1201 gagtttggtg aacgcgaatg ggatgaagta gttgtgcgaa catatgccac cgaagcaaac
     1261 tttcaacgtt tagaccgtgc tgaagctttg gcaaaagaaa aaggatgcac agccgcgcaa
     1321 atcgcattag cgtatgtaat gaaccaaccg atgaatgttt tttcagttgt tggttctcat
     1381 agtattgaaa agttcaaagc aaacattgaa gccgctgaat tgaaactcac caaccaagaa
     1441 ttggcttggc tggacctgaa agtagaaaat ctataatgag caaacaaacc ctaaaaaaca
     1501 atttctttga gcttgaatat ctcacacatt ctttacgtat ctctagcata accccgcatg
     1561 gcaaaaccaa tttgctagcc gatctaagtc atcagccgcc cgtcccaacc ccttacggcg
     1621 atttctattt gcgaggcggg catcgtttat ggcatgcccc cgaagccatg ccgcgcactt
     1681 atgtccccga tactggtgaa ataaagatca ctaatattcc caatggtgtt gtgcttgaaa
     1741 ccgaaacaga gcccggtgcc aacattcgca agcggatcga aattaaactt gcagtagata
     1801 caccctctct cacccttacc cacacccttt tcaatgacgg cttgtggact ctcgaactgg
     1861 caccatgggc gatcactcaa tttcgattgg gtggaatagc aattttgccc atgcccactg
     1921 aaaaagtgga tgatgccgca ttacttccta accgccaaat atctttttgg acctacaccc
     1981 gcctcaacga caaacgccta agcctgcgcg atgaattcag cctttttcac gccgagtctt
     2041 ctttgccgcc tttcaaaatg ggctatttca acccgcatgg ctggctggca tattatatag
     2101 acggcgttct cttcaagaaa acgtttaacg catcaacact tgcaccctac ccagataaca
     2161 attgcaatgc cgagcttttc tgtaacgatg aatttgtgga attggaaagt ttaggcccac
     2221 tcacaaagct ggaacccggt gcatcggtgc aacatcagga aacatgggaa atccttgatg
     2281 caaatgcatt accgcaagat attcagaatt tgattctgaa atagcccgca ctaaaaaaat
     2341 ttaggcgtat gcccggtcaa agcaaggaag cgctccaact ccgctttggt taaagtcaag
     2401 gtggctgtgt taaccaaggg atggcaaaga aagttctctg cctgccaaat ttcgttgtcg
     2461 atccacaatt cgacgatatg ttctgtatca tttgccaagc ccatcatcgt cactgagccg
     2521 cctgccacac ctaagaggcg cagcaagttc tgttcagatg caaagcgcaa atgagggacg
     2581 ccaaatagcc ctgagagttc atcgagccga accgtctttt cgcaagccgt gactgctaaa
     2641 aagaagcgac gggctttttt atcacataga aaaaggtttt tggtgctgac tgcatccacg
     2701 ccaatatggt gggcatccgc ttcggcacag gtgaagacgg ctggatgttc catgcgagaa
     2761 taaacaaacc ccttagcgtt caaaaattca aggaaggcat tttcagattc gatcataagc
     2821 gatagggttg gcatgatacc gcgaaaggcg cgtaaaggcg agcccaactt tcctctagat
     2881 ttaacaatca caacaagcac tttgtggtac agtgtctcta acttttccca aggagccaaa
     2941 ccatggaaca aaaagctggt gctcagatcg g
//
LOCUS       DAOQFT010000300         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_19992_length_2971_cov_5.750343, whole genome shotgun sequence.
ACCESSION   DAOQFT010000300 DAOQFT010000000
VERSION     DAOQFT010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19992_length_2971_cov_5.750343"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PLR93_08295"
     CDS             complement(<1..1311)
                     /locus_tag="PLR93_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27004.1"
                     /translation="MAEEKKKDEEKKDEKATPKDNIVTTKHSVKIGGKTIKYTVTTGT
                     MVLKEEVNEKDSEIEKPRAQIFFTAYTMDGVRDKAKRPITFSFNGGPGSSSVWLHLGV
                     LGPRRVVMTNDGEMPKPPFKLTDNEFSVLDETDLVFIDPMSTGYSRPVEGEKSKDFHT
                     FQKDIASVGDFIRLYTTRYNRWLSPKFLAGESYGTTRAAGLSGYLADRHGMLLNGLML
                     VSSVLDFTTVDFNHNNDLPYILFVPGYTATAWYHGKLDKKKPLKTWLKESEEFALGEY
                     ATALMKDGALSKEERRKVAEKLARLTGISADFVERGNLRIHTFHFFKELLRDEGKTVG
                     RLDSRYTGIDRLGVTETFEYDPLFAQVNGPYTAAFNDYIRTELKFETDSTYEILSDKV
                     WRQWSYSYFENQYVNVAETLRAAMTFNKYLKVFVANGYYDLGTPY"
     gene            complement(1323..2405)
                     /locus_tag="PLR93_08300"
     CDS             complement(1323..2405)
                     /locus_tag="PLR93_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27005.1"
                     /translation="MEEIAQTTRGENIIPPLLDRTWKRVLFGLFVVTAPIFNFSLIEL
                     MKPEWQDGRFTSYLYLFLLPEASWGFFPLLLYAILSYLLLLWDSEQFADSMIIRLGIY
                     GGTLLALQYSILSFIATFSVSPMVLAVILAYSAPLLLPKLYFWLRPQLDKLFKMYRLA
                     MIIIILIIIALVLWGFFFVLAIFGIASPFWSFLVAFQASCWLLKHYETKFSFTKGLGV
                     VAWISAYAFALRFNIIKMFELYNALPTEPPNCYIATAAAKGHPRFVGSREVTLASGKS
                     MRVNRQLQRLKALEIVWMGVSAPSHRMMRQVYDVIGKRLAKQIQNPLLADIAYLILVP
                     VELLSFFVLKSIVPEIQTLSERLYRS"
     gene            complement(2418..>2971)
                     /locus_tag="PLR93_08305"
     CDS             complement(2418..>2971)
                     /locus_tag="PLR93_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNH27006.1"
                     /translation="YELVQAALAASFDVRPVPGPSAPISALTVSGLPTDSFLYLGYLP
                     HKSSERHNRLNEVSEQPYTLIFFETPHRIADSLEDIRSTLGNRRICVAREMTKMYEEF
                     WRGDVSGAIEYFNSKEPRGEFTLVIEGKKKEERTVWTETELTEAIKRELQEGRSAKEI
                     SAELAEKSGWNKKEVYALVNQVK"
BASE COUNT          782 a          673 c          756 g          760 t
ORIGIN      
        1 ataaggtgtg ccgaggtcat agtagccgtt ggcgacgaag accttgaggt atttattgaa
       61 ggtcatcgcg gcgcggagcg tttcagcgac gttgacatat tgattctcaa aataactgta
      121 cgaccattgc cgccaaactt tatcgctgag gatctcatag gtcgagtcgg tttcgaattt
      181 gagctcggtg cggatgtaat cgttgaatgc cgccgtgtaa ggaccattga cctgtgcaaa
      241 gagcgggtcg tattcaaaag tttcggtcac accgaggcgg tcgatgccgg tgtaacggct
      301 gtcgaggcga ccaacggttt tgccttcatc gcgcaaaagt tccttgaaga agtggaaggt
      361 gtgaatgcgc aagttgccgc gttcgacgaa gtctgctgag atgccagtga ggcgggcgag
      421 tttctcagct acttttcttc tttcttcttt agagagcgca ccatctttca tcaaagcggt
      481 agcatattca cccaaagcaa attcttccga ttccttgagc caagtcttga gcggtttctt
      541 tttatccaac ttgccgtgat accacgcggt ggcggtgtaa cccggaacaa agagaatgta
      601 aggcaggtcg ttgttgtgat tgaaatcaac cgtggtgaaa tcgagcacag atgaaacgag
      661 catcagtcca ttaagcaaca tgccatgacg gtctgcaaga tagccggaga gtcccgccgc
      721 gcgtgtggtg ccataggatt cgcccgcgag gaatttcggt gagagccagc ggttgtaacg
      781 tgtggtgtag agccgaatga aatcacctac cgaggcgata tctttttgga aggtatgaaa
      841 atctttcgac ttctcgcctt ccacggggcg gctgtagccg gtgctcatcg ggtcaatgaa
      901 aaccaggtcg gtttcatcga gcacagaaaa ttcgttgtct gtcaatttga agggcggctt
      961 gggcatctcg ccgtcattgg tcatcaccac acggcgcgga ccgagcacac cgaggtgcaa
     1021 ccaaacggat gaagagccgg gtccgccatt gaaggagaaa gtgatcgggc gcttggcttt
     1081 atcgcgcaca ccgtccattg tgtaggcagt gaaaaaaatc tgtgcgcgcg gtttttcgat
     1141 ctcactatct ttttcgttca cttcctcttt caaaaccatc gttccggttg tgacggtgta
     1201 cttgatggtc ttgccgccga tcttcaccga atgtttggtg gtgacgatgt tatctttcgg
     1261 agttgctttt tcgtctttct tctcttcgtc tttctttttt tcttcagcca tgtgagtctc
     1321 ctttatgagc gatataaacg ctcagacaaa gtttgaattt cagggacgat tgattttagt
     1381 acaaagaacg acaataattc aacaggaacc aaaataagat atgcaatatc agccagtagc
     1441 ggattttgga tctgcttcgc cagccgtttg ccaatgacat catacacctg ccgcatcatt
     1501 ctatgacttg gagcggatac gcccatccaa acaatttcca acgctttgag cctctgcaac
     1561 tgacgattga cccgcatgga ttttccgctt gccaatgtca cctctcgcga accgacgaag
     1621 cgtggatgtc ccttcgcggc ggcggtggcg atgtaacaat tgggcggttc ggtaggcaag
     1681 gcgttataca actcgaacat tttgatgatg ttgaagcgca gggcaaaggc atacgctgaa
     1741 atccaagcga cgacgccaag tccttttgtg aaagaaaact tggtttcgta gtgtttcaaa
     1801 agccaacaag aagcttggaa ggcaacaagg aaagaccaga agggtgaagc gatcccaaag
     1861 atcgctaata caaagaaaaa tccccacaga acaagggcta tgatgatcaa tataatgatg
     1921 atcatagcaa gccgatacat tttgaataat ttatccaatt gaggtctcaa ccaaaagtac
     1981 agctttggta ggaggagagg tgcagaatat gcaaggataa cagccaaaac cattggcgaa
     2041 acactgaatg tagcaatgaa cgacaaaatg ctgtattgca gagcaaggag tgtgccgcca
     2101 tagatgccaa ggcggatgat cattgaatca gcgaattgtt cgctatccca tagtagcaag
     2161 agataactga gaattgcata caaaagtaaa gggaagaatc cccaagaagc ctctggcaaa
     2221 aggaagagat agagatagga tgtaaaccga ccatcctgcc actctggttt catcagttca
     2281 atcagcgaaa aattaaagat cggggcagta acaacaaaaa gcccaaagag aacacgcttc
     2341 caagtcctat ctaacaatgg cggaataata ttttcccctc tggtcgtttg agctatttct
     2401 tccatgcatg gctcctatta cttgacctga ttcaccaagg cgtacacttc ttttttattc
     2461 caaccgcttt tttctgccaa ttctgcagag atctcttttg cagatcttcc ttcttgaagt
     2521 tcgcgtttga tcgcttctgt gagttcggtt tcagtccaaa cggttctttc ttctttcttc
     2581 tttccttcaa tgaccagagt aaattcaccc ctaggttctt tgctgttgaa atattcgatc
     2641 gccccactca catctccgcg ccaaaattct tcgtacatct tcgtcatctc gcgggctacg
     2701 cagatgcgac gattgccgag ggtagagcga atatcctcga gcgagtctgc gatgcggtga
     2761 ggagtttcga aaaagataag agtataaggc tgttcgctga cttcgttgag tcggttatgt
     2821 cgttcgctgg atttgtgggg aaggtagcca aggtacaaaa atgaatcagt ggggagacca
     2881 gaaacggtca aggcggagat gggagctgag ggaccgggaa caggtcgaac gtcgaaagaa
     2941 gcggcgaggg cggcttgaac gagctcatag c
//
LOCUS       DAOQFT010000301         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20017_length_2969_cov_7.049417, whole genome shotgun sequence.
ACCESSION   DAOQFT010000301 DAOQFT010000000
VERSION     DAOQFT010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20017_length_2969_cov_7.049417"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLR93_08310"
     CDS             <1..279
                     /locus_tag="PLR93_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNH27007.1"
                     /translation="MPEFEAWIVDQKRKTNLNVVGTDSTGDVSLMNHTLARPVAVVLG
                     NEAKGMSVALKNLCDYVVSIPVEGAVNSLNVACAGSIILWDVYKNSHR"
     gene            complement(322..1392)
                     /locus_tag="PLR93_08315"
     CDS             complement(322..1392)
                     /locus_tag="PLR93_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27008.1"
                     /translation="MKRLFPFILTTIFILQACGGNAPTEESAAAPQTANTEVAAATAD
                     VAPATAEPIVHVDIPTEGTVSRASAHDNEESTNSGNKDVIFGDDFAKNRFERPFTAND
                     MTYLPDLDIVDFSITSDDSFFYIRIDLVGLDLASQSLTGFYGVEFDKNGDGRAEIFLV
                     TQPPYKTEFTADNVFLFIDANGDVGSEQAAKPDASNGDGFDSLLFDLNDSIHPEDPDL
                     AWVRFIDDTKPAIEIAYKKWLFKDGNENFMWSVTASDELINPAEYNFHDAIAETEAGS
                     PTKSNPNYPLKGLAAIDNTCRVPFGFEAKGNEPLGCSVNNIPPEPEEEIDFCTQFPSA
                     CERLPEPPPLQQFFDKEDLKGF"
     gene            1642..>2969
                     /locus_tag="PLR93_08320"
     CDS             1642..>2969
                     /locus_tag="PLR93_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNH27009.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVIPQILEKLNVSAQALTERLDATLRASPKANIFGGGAGQIFITPRVKRIIDLANEEA
                     NRLKDEYISTEHIFLAILTERNTPAARILESAGLTRDRVYDAIQDLRGGQRVTDPQAE
                     TRYRTLEKYSRDLTQLAREGKLDPVIGRDNEIMRLIQILSRRTKNNPVLIGEAGVGKT
                     AIAEGLAQKIASNDVPEILSGKRVVALDLGAMIAGSRFRGEFEERLKAVMEEVQRAKG
                     DVILMIDELHTVVGAGAAQGAMDASNMLKPALARGELQTIGATTLDEYIKHIEKDAAL
                     ERRFAPIYVDEPSVEDTIKMLHGLRDKYEAHHKVRFSDEALSAAAHLAVRYVTDRHLP
                     DKAIDLMDEAAAKLRVALYSMPPDLKALKTEIDKLAAEEEQAGLERDYERA"
BASE COUNT          737 a          646 c          833 g          753 t
ORIGIN      
        1 atgccggagt tcgaagcgtg gatagtggac cagaagcgca agaccaatct caatgttgtg
       61 ggaaccgatt cgacgggaga tgtctctctg atgaaccaca cccttgcaag acctgttgcg
      121 gtagttttgg gtaatgaagc caagggcatg agtgtggcgc tcaaaaatct atgtgactat
      181 gttgtgagca tcccagtaga gggagcagtc aattcgctaa atgtggcgtg tgcggggtcg
      241 atcattttgt gggatgtata taagaatagc catagataaa aaactgcgcg gataaagtcc
      301 gcgcagtttt tatctatcta attaaaatcc tttcaaatct tctttatcaa aaaactgttg
      361 aaggggtggc ggttctggca aacgttcgca tgccgatgga aactgtgtac agaagtctat
      421 ttcttcttcg ggctccggcg gtatgttgtt tactgaacat cccaagggtt cgtttccttt
      481 cgcttcgaaa ccaaatggga cacggcatgt attgtctata gcggcaagac ctttgagagg
      541 ataattgggg ttggactttg ttggtgagcc ggcttctgtt tctgcgatgg catcatggaa
      601 gttgtattca gcagggttga ttaattcgtc gctggcggta acactccaca tgaaattctc
      661 attgccgtct ttgaagagcc atttcttata ggcgatctcg atagcaggtt tggtgtcatc
      721 gatgaagcgc acccatgcca gatcggggtc ttcggggtgg atgctatcgt tcaggtcaaa
      781 gagtagggag tcgaaaccgt cgccgttgga agcatcgggt ttggcggctt gttcgctgcc
      841 tacgtctcca ttggcatcaa taaagagaaa gacattatca gcagtgaatt ctgttttata
      901 aggcggttgg gttacaagaa aaatttcggc gcgaccatcg ccgttcttgt caaactccac
      961 accgtagaac ccggtcagag actggctggc aagatcaagt ccgaccagat cgatgcggat
     1021 gtaaaagaag gagtcgtcac tggtgatgga gaaatccacg atatcgagat cggggagata
     1081 ggtcatatcg ttggctgtaa aggggcgttc aaaacgattc tttgcaaagt catcgccaaa
     1141 gatcacatct ttgtttccag agttggtgct ttcttcgttg tcatgagctg aggctcggct
     1201 aacagtacct tcggttggga tgtcaacatg tacaatcggt tctgcagtag ctggtgcaac
     1261 gtctgctgtg gcagcagcga cctctgtatt ggcagtttgg ggagcggcgg ctgattcttc
     1321 tgtgggggca tttccgccac aggcttgcaa aatgaaaatt gtagtgagga taaaggggaa
     1381 aaggcgtttc atgggtctcc taacgatata ttggagataa tagtttattc ttttctggcg
     1441 ctacacccaa aaaagtaagg aatagggtat tactttagtg atggtcgccg aaatctaaca
     1501 ctcttcttat gccatactga caattcgccg atacggcgtt tgttataatg ccagcattgg
     1561 gtggacgata gacaatggac gatagaccat aaaaccatgg tctgcgatcc atggtctgtg
     1621 gtctgtccgg aggatacccc tatgatgcga tttgaccgat ttacagagag agcacaagaa
     1681 gccgcccagc gtgctgcgga gattatccaa cgctatgggc acaatcaaat tgataccgaa
     1741 cacattctgc tggcgctgat tgaacagcct ggtggagtga ttccccaaat tcttgaaaaa
     1801 ttgaatgtca gcgctcaagc gctcaccgaa cggctggatg ccaccctgcg cgccagcccc
     1861 aaggcgaaca tcttcggcgg cggagcggga cagattttca tcaccccgcg cgtcaagcgg
     1921 attatcgacc ttgccaacga agaagccaac cgtctcaaag acgaatatat ttcaaccgaa
     1981 catatctttt tggcgattct cactgaacgt aatacccctg cggctcgcat tttggaatct
     2041 gcaggattaa cccgtgaccg tgtgtatgat gccattcagg atctgcgcgg tggtcaacgg
     2101 gtgaccgatc cgcaagcgga gacgcgttac cgcacccttg aaaaatattc gcgtgacctg
     2161 acgcagcttg cccgtgaagg taagcttgac ccggtcattg ggcgcgataa tgagatcatg
     2221 cgcctgattc agattctgtc tcgccgtacg aagaacaatc cggttttgat cggtgaagcg
     2281 ggtgtgggga agacagccat tgcagaaggt ttggcgcaga agatcgccag caacgatgtg
     2341 cccgagattc tatctggcaa gagagtggtt gcgctcgatt tgggcgcgat gattgccggg
     2401 tccagattta gaggcgagtt cgaagaacgt ctcaaggctg tgatggaaga agtccagcgt
     2461 gcgaagggtg atgtcattct gatgatcgat gagttgcata cggttgtggg ggcgggtgcg
     2521 gctcaaggcg cgatggacgc tagcaacatg ctcaagccgg cgttggcgcg tggtgagtta
     2581 cagaccattg gcgcgactac gttggatgag tatatcaaac acatcgaaaa agatgcggcg
     2641 cttgaacgtc gttttgctcc gatctatgtg gatgagccaa gcgtagaaga taccattaag
     2701 atgttgcatg gtttgcgtga caaatatgaa gcgcatcata aagttcgctt ctctgatgaa
     2761 gcactttctg ctgcggctca cttggcggtt cgttatgtga ccgatcgtca cttgcctgat
     2821 aaagccattg acttgatgga tgaagccgct gcgaaactgc gcgtggcgtt gtactccatg
     2881 ccgcctgatt tgaaggcgtt gaagactgaa attgacaaac tggctgccga agaagaacaa
     2941 gctggtttgg aacgtgatta cgaacgcgc
//
LOCUS       DAOQFT010000302         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20076_length_2964_cov_6.975937, whole genome shotgun sequence.
ACCESSION   DAOQFT010000302 DAOQFT010000000
VERSION     DAOQFT010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20076_length_2964_cov_6.975937"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PLR93_08325"
     CDS             <1..1055
                     /locus_tag="PLR93_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH27010.1"
                     /translation="HMNLTRFDEKIAKTIPSLMKMGIQTLKVFTAYNGRLRLDDGGIF
                     KALQIARDNGMLVMAHCENGDVVETLTEKALAEGHTEPIWHALTRPAWGAVESSLRMA
                     AMAEQANAPVYIVHMNAGGEVDMLQYARERGVKVMGETCPQYLFFTQDHLSRDDGAKW
                     ICSPPMRTEQDNAKLWDGLRQGILQTIGTDHCPFYYDGTQPIVYEGSEIAIPGKELGR
                     ENFTKIPNGLPGIQDRMPVLWTYGVNAGYLTPNQFVAYMSTNPAKIFGLYPRKGALVP
                     GADADIVIWDPEKQVRYGVAHSHQRTDYNLYEGWELKGYPEKVFLRGKLIVEGDEWKG
                     KRGDGKFLKRNEGEVL"
     gene            1110..1487
                     /locus_tag="PLR93_08330"
     CDS             1110..1487
                     /locus_tag="PLR93_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27011.1"
                     /translation="MPTKKPIIHCDALVVHCMDYRLQKFIQPWITVRFGYDNFDIISL
                     AGSVHDYEMVLKYVQLAVQIHTIETVCLINHEDCRAYGRDGTYKRHKHDLTDAQKKIK
                     ALFPHLKVETFYLHLDGTFEPIS"
     gene            1487..2524
                     /locus_tag="PLR93_08335"
     CDS             1487..2524
                     /locus_tag="PLR93_08335"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNH27012.1"
                     /translation="MTQFLTLSPHSPSPELIRQAADVILRRGLVAFPTETVYGLGANA
                     LDEEAVAKIFHAKGRPSNDPLIVHVASLDDVKLVAAEIPPIVEKLAAAFWPGPLTLVL
                     PKQAHVPASVTAGLDSVAVRVPSHPVALALIRASGVPIAAPSANRFGHTSPTTAQHVI
                     DDLNGRIDMILDGGATSVGVESTVLDVTRDPAMLLRPGGVSREAIEKIIGVVELRNNR
                     PNSDDEAQVSPGLLGKHYTPNAELLLCKGERDSALEKLLGLAKQAQAEGRSVGLLLAN
                     EDIASFESACIPYPLGASNDLEALARNLYAGMRALDDQGVKLILTRDFGKQGLGLAIH
                     DRLLRAASQII"
     gene            2541..2903
                     /locus_tag="PLR93_08340"
     CDS             2541..2903
                     /locus_tag="PLR93_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27013.1"
                     /translation="MSHTCEAIVVHCMDYRLQSYINQWLETNLGKGSYDRAVMAGGVY
                     DVYDVIRQVDISARLHGITKVVLINHEDCGMYGPGGTEERHEEDLDKAEDKIRRLFPH
                     LEVDSYYLNLDGTFERKS"
BASE COUNT          779 a          722 c          755 g          708 t
ORIGIN      
        1 tccatatgaa tctcacccgc ttcgatgaaa agattgcgaa aacgattccg tcgttgatga
       61 agatgggtat tcaaaccctg aaagtattca ccgcctataa cggacgtctg cgtctcgatg
      121 atggcggcat tttcaaagcg ctgcaaatcg cccgcgacaa tggcatgctc gtcatggcac
      181 attgcgaaaa tggtgacgtg gtcgaaactc tcactgagaa agcacttgct gaaggtcaca
      241 ccgaaccgat ctggcatgcg cttacccgcc cggcttgggg agctgtggag tcaagcttac
      301 gcatggctgc catggcagaa caagccaatg cgcctgtgta catcgtccac atgaatgcgg
      361 gcggcgaagt agatatgctc caatatgcgc gcgaacgtgg cgtgaaagtg atgggtgaaa
      421 cttgtccgca atatttattc ttcacacaag atcatcttag ccgcgacgac ggcgccaaat
      481 ggatttgctc tccacccatg cgcactgagc aagataacgc taaattatgg gatgggctgc
      541 gtcagggaat tttgcaaaca ataggcacgg accattgccc cttctattat gacggaaccc
      601 agcccatcgt ctacgaagga agcgagatcg ccatccccgg caaagaactc gggagagaga
      661 acttcaccaa aatcccgaac ggtttgcccg gcatccaaga ccgcatgcca gtgctttgga
      721 cctatggtgt aaatgcaggt tacctcactc ccaatcaatt cgtggcatat atgtctacca
      781 acccggcgaa aatatttggt ttgtatccgc gcaaaggagc gcttgtgcct ggcgccgatg
      841 cagacatcgt catttgggat ccggaaaaac aagtgcgcta tggggttgcc cactcgcatc
      901 aacgtacaga ttacaatcta tatgaaggct gggaattgaa aggctatcct gaaaaagttt
      961 ttctgcgcgg caagctcatc gtagagggcg atgaatggaa aggaaaacgt ggcgacggaa
     1021 aattcttgaa aagaaatgaa ggcgaagttt tatagtctac ctgctcaata ctgaattgag
     1081 caacattaca tattagaggt aaaaacatta tgccaaccaa aaaaccgatc attcactgcg
     1141 acgcactcgt tgttcactgc atggactacc gcctgcagaa attcatccaa ccgtggatca
     1201 ccgtccgctt tggatacgac aacttcgaca tcatctcact ggcaggtagt gtacatgact
     1261 atgaaatggt attgaagtat gtccaacttg ctgtgcaaat ccataccatt gaaaccgtct
     1321 gtctgatcaa ccacgaagat tgccgcgcct atggacgaga cggcacatat aaacgccaca
     1381 aacatgacct gaccgatgct cagaaaaaaa tcaaagcttt atttccacac ttgaaagttg
     1441 agacgttcta tctgcacttg gatggaacct tcgaaccaat ctcttgatga cccaattcct
     1501 gaccctttcg cctcattccc caagccctga actgattcgc caagccgcag acgtgatcct
     1561 acgccgcggc ttggttgcct tccccaccga aaccgtatac ggacttggag ccaacgctct
     1621 cgatgaggaa gctgtggcaa aaattttcca tgccaaaggg cgcccttcca atgacccgct
     1681 gattgtgcat gtggcttcgc ttgatgatgt aaaactcgtt gccgctgaga tcccccccat
     1741 cgttgaaaag ctcgccgccg ctttctggcc cggtccgctg acgttggtgc tgccaaagca
     1801 agcgcatgtc cccgcatctg tcacagcagg gttagattca gttgcagtac gtgtcccgtc
     1861 acaccctgtg gcgttggcgc tcatccgtgc gagtggtgtg cctattgctg cgccatctgc
     1921 aaatcgattc gggcatacca gtccgaccac ggctcaacat gtgatagatg atctcaacgg
     1981 tcgcattgac atgatcctcg acggcggtgc aacttcagtg ggagtcgaat cgacggtgct
     2041 ggatgtgacc cgtgacccgg caatgcttct acgcccgggc ggggtttcgc gcgaggcaat
     2101 cgagaaaatc attggtgtag ttgagttacg aaataaccgc cccaactcgg atgacgaggc
     2161 gcaagtctcg ccgggattat tggggaaaca ttacacgcca aatgcagaat tgcttctatg
     2221 caaaggcgaa agggattcag cgttggaaaa actgctggga ttggcgaaac aggcacaagc
     2281 ggaaggaaga agcgtgggac ttcttcttgc aaatgaagac atagcgtctt tcgagtccgc
     2341 ttgcattccg tatccgcttg gtgcatccaa tgacctcgaa gcgcttgcgc ggaatcttta
     2401 tgcgggcatg cgagcgttgg acgatcaagg tgtgaagttg attctgacac gtgattttgg
     2461 aaaacaaggt ttggggctgg ctattcatga ccgcttgtta agagcagcga gtcagatcat
     2521 ttaattttcg gagggaagaa atgtctcata cgtgtgaagc aattgtggtt cattgcatgg
     2581 attatcgttt acagtcgtat atcaaccaat ggttggaaac gaatttaggc aaagggagtt
     2641 acgaccgcgc cgtgatggcg ggtggcgtgt acgatgttta tgatgtgatc cgtcaggtgg
     2701 atatttcggc aaggttgcat ggtattacaa aggtggtgct gatcaatcat gaagattgcg
     2761 gtatgtatgg tcctggtggg acggaagaac gccatgaaga agaccttgat aaagccgaag
     2821 ataaaattcg caggttattc ccacaccttg aagtagattc atattaccta aacttagatg
     2881 gaaccttcga gcgtaagtct tagccaataa ggaacatttt cagaacggct ttcgtctttt
     2941 tctcaaccct tttatttgga gcat
//
LOCUS       DAOQFT010000303         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20121_length_2961_cov_7.062629, whole genome shotgun sequence.
ACCESSION   DAOQFT010000303 DAOQFT010000000
VERSION     DAOQFT010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20121_length_2961_cov_7.062629"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLR93_08345"
     CDS             complement(<1..526)
                     /locus_tag="PLR93_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27014.1"
                     /translation="MFQAAPPTRFDLQFSIAGIPVRVHPLFWVIAILLGSGSSNLLMI
                     PVWIAIVFISILVHELGHAFAFRRFGQPSRILLHFSGGLTIPESMPWGGGYATVSLTP
                     NQHIFVSLAGPFAGFLLAGLILAVGVAMGGTLIFSTILKIIPFPIVMMPYGSGILTDI
                     FVTFLWVNIFWGIIN"
     gene            complement(538..1305)
                     /gene="fabL"
                     /locus_tag="PLR93_08350"
     CDS             complement(538..1305)
                     /gene="fabL"
                     /locus_tag="PLR93_08350"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HNH27015.1"
                     /translation="MNFQNKIALVTGSGRGIGKAIALNFASQGADIVVNFFRNRAPAE
                     ETVREIEKLGRRALLVKADVGDLDDLNRLFDEVEKEFGGLDIFIHNAASGYNRPAMEQ
                     KPKGWDWTMNINARALLFAAQRATPLMEKRGGGSIVSISSAGSVRVLPDYVVVGASKA
                     ALEALTRYLGVELIQRGIKVNAVSPGVVETEALHHFSSMGGQENILQKFIERVPAGRL
                     ITPEDVAEVVAFLCSPASSMIVGQTIVVDGGYTLPIQ"
     gene            complement(1321..1860)
                     /locus_tag="PLR93_08355"
     CDS             complement(1321..1860)
                     /locus_tag="PLR93_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNH27016.1"
                     /translation="MPKEQEINSIERTAEECQGKLHPKAIVGLQLFNQGEFFEAHEEL
                     ETAWRDETSSIRDLYRGILQIAVTYLHITRGNYDGAVKVYGRSLKWLQGWGDICRGVN
                     IKKLRRDAETVMQEVTRLGWERIREFDRSLFQPIQWNEKRLWICDRCGSEMYEKNCKV
                     SCPTCGNRFDCSDLNLYFD"
     gene            1918..2322
                     /locus_tag="PLR93_08360"
     CDS             1918..2322
                     /locus_tag="PLR93_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27017.1"
                     /translation="MLFIALIVCILISTGSFVWGYSQAGYEDAVRWLVAFGVLWFLAE
                     WRKWKWFSAPAVLFSLFLAGYGVWFKFVPGLMFNGAVFSVLAWNITEFQEKLRLLPQR
                     EDKQGMTRRHLIRIGTLSIGAVLIALFLGIRG"
     gene            complement(2305..>2961)
                     /locus_tag="PLR93_08365"
     CDS             complement(2305..>2961)
                     /locus_tag="PLR93_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27018.1"
                     /translation="SLSGRSNMENSFAMRNSYYVKYTVRITNYNPLPMNTFTYKRIVI
                     IGTTSSGKSTLAEKLSTILGVNFIELDALHWEPNWKEADTLVFRQRVDLATQAIGWVV
                     AGNYSAVRDIVWNRAEVVIWLDYSFHIVFWRLLTRTVRRAITQEVLWNGNRESFWWHL
                     KLWSEESLFHWLFKTYWRRKREIPILLSKPENRHLTILHFRHSQEAEAWLKGLSPTNA
                     "
BASE COUNT          794 a          688 c          693 g          786 t
ORIGIN      
        1 gattgatgat gccccagaaa atgttgaccc acaaaaaggt cacaaagata tctgtcaaga
       61 taccgctgcc gtatggcatc atgacgatgg ggaaggggat gatttttagt atggtagaga
      121 aaatcaatgt gccgcccatt gcaacaccca cagcgaggat caagcctgcg agcaagaatc
      181 cggcaaacgg tccggcaagt gagacgaaga tgtgttgatt gggtgtcagg ctcacggtgg
      241 catatccgcc gccccatggc atcgattcgg ggatggttaa tccaccagaa aaatgcagga
      301 gaatgcgcga tggctgacca aagcgcctga acgcaaatgc atgaccaagt tcatgaacaa
      361 gaatagaaat aaaaactatt gcaatccaaa ctgggatcat caacaaattg cttgagccag
      421 agccaagcaa aatagcaatc acccaaaaga gcggatgcac gcgcacggga atgccggcga
      481 ttgaaaactg taaatcaaaa cgagtgggag gagcagcttg aaacataata tgtcctgtta
      541 ttgaatgggt aaagtatagc cgccatccac gacgatggtt tgaccaacga tcatggacga
      601 agcgggcgaa cacaaaaacg ctaccacttc tgcaacgtct tcaggagtga tgagacgacc
      661 agccggtacg cgctcgataa atttctggag gatgttttcc tgcccgccca tggatgaaaa
      721 atggtgcaac gcctctgtct ccaccacgcc gggtgacacc gcgtttacct tgatgccacg
      781 ctgaatcaac tccacaccta aatagcgcgt gagggcttcc agggctgcct tactggcgcc
      841 caccacaaca taatcgggta acactcgcac cgaccctgca ctcgatatgc tgacgatgct
      901 cccaccgccg cgtttttcca taagaggcgt ggcacgctgt gcagcgaata acaatgcacg
      961 cgcattgatg ttcattgtcc aatcccagcc tttgggcttt tgttccattg cagggcggtt
     1021 gtagcctgat gcagcattgt gaatgaagat atccaagccg ccaaattctt tttctacttc
     1081 atcgaagagg cgatttaagt catctaaatc gcctacatcg gctttaacca gcaaagcacg
     1141 cctgccaagt ttttcaatct cgcgaactgt ctcttcggca ggagcacggt tgcgaaagaa
     1201 attaacgaca atatctgccc cctgactggc aaaattcagg gcgatggctt ttccaattcc
     1261 gcgccctgag ccggtgacga gggcgatttt gttttgaaaa ttcattgggt ctccagagca
     1321 ttaatcaaag taaagattta aatccgaaca gtcgaagcgg ttgccacagg ttgggcaact
     1381 cactttgcaa ttcttttcat acatctcact gccacagcga tcgcagatcc acaggcgctt
     1441 ttcgttccat tgaataggtt ggaataaaga cctatcgaac tcacgtattc tttcccaccc
     1501 caaacgagtc acttcttgca taacggtctc tgcatcacgg cgaagtttct tgatgttaac
     1561 accgcggcag atgtcgcccc agccttgcaa ccacttcaag ctccgcccat agactttaac
     1621 tgcaccgtca tagtttccgc gtgtaatatg cagataggtt accgcgattt gaagaatgcc
     1681 gcgatataag tctcgaattg agctggtttc atctcgccaa gcggtttcca actcttcatg
     1741 ggcttcaaaa aattctcctt gattgaataa ctgaagacct acaatggctt tcggatgtaa
     1801 ctttccttgg cattcttctg ctgttctttc aatagaattt atttcttgtt cctttggcat
     1861 atcgagagta taacatgcag gttttatacg aatggtaaaa tttgtgttgg aggaaaattg
     1921 ttatttattg cgcttatcgt ctgcatcttg atatcaacag gctcgttcgt atggggatac
     1981 tctcaagctg gctatgagga tgccgtccgc tggctagtag ctttcggtgt tctttggttt
     2041 ttggcggaat ggcgtaaatg gaagtggttc tctgctccgg ctgtgctgtt tagtctgttt
     2101 ttagctgggt atggcgtttg gtttaagttt gttcctgggc ttatgtttaa cggtgctgtg
     2161 tttagtgtat tggcatggaa tattactgaa tttcaagaaa aattgaggct actacctcaa
     2221 cgagaagaca aacaaggtat gacaagacgc cacttgatac gcattggaac tctttctatt
     2281 ggtgcggtct tgattgcgct ttttctaggc attcgtgggt gacaaaccct ttagccatgc
     2341 ttccgcttct tgtgagtggc gaaaatgcaa tattgtgagg tgtcgatttt caggctttga
     2401 caacaagatg gggatttcac gttttctgcg ccaataggtt ttgaacagcc aatgaaacaa
     2461 agactcttca gaccagagtt tgaggtgcca ccaaaatgac tcacgattcc cgttccaaag
     2521 aacttcctgc gttatagcgc ggcgaacggt gcgggtgagt agacgccaaa atacaatatg
     2581 gaaggaataa tccaaccaga tgacaacttc tgcccggttc caaacaatat cgcgcacagc
     2641 actgtaattc cctgcaacaa cccaacctat tgcctgagta gcaaggtcta ctctttggcg
     2701 aaaaacaagc gtatcagctt ctttccagtt cggctcccaa tgaagagcat ctaattcaat
     2761 aaaattcacc cctagtatag tagagagttt ttctgctagc gtagatttgc ctgaagatgt
     2821 ggtgccaata ataacaatac gtttgtaagt gaatgtgttc atgggcaggg gattataatt
     2881 cgtgatacgt accgtgtatt ttacatagta tgaatttcgc attgcgaagg aattctccat
     2941 gttcgatctt cccgataatg a
//
LOCUS       DAOQFT010000304         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20260_length_2949_cov_6.518659, whole genome shotgun sequence.
ACCESSION   DAOQFT010000304 DAOQFT010000000
VERSION     DAOQFT010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20260_length_2949_cov_6.518659"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLR93_08370"
     CDS             <1..1221
                     /locus_tag="PLR93_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27019.1"
                     /translation="GLSHLSKLSALSSLSSLSGLSSLSPADPESPRNARTRELLGALK
                     EAGVDHVTMSDLQEMKIHNLSADYVREMRGLGIEPDGIGEWINLRIHDISPRYVRDLR
                     DVGVTDLDVDEIVDLYLQGVSAKFIGGLRDLGINDLDIDEIKELNNHDISPKFIAEMR
                     SLGFKDLDVDELVELAIHDVSPKFVAEMRDLGLKDLDVDELVELANHDVSPKFVAELR
                     ALGLKDLDVDELIEFANHDVSPKFIAELRSLGFTDFDADDLIELANHDVTPDFIAAMR
                     EVGLKDLAADEFIDLRNQGITPGYVRAMVEAGLKDLEVDQLEELKIHNVTPKFVREVL
                     EMGFKDVDIDELVELSIHHVTPRFMRDVREKLGEDLTLEQILDIRLHGIDEDLMEELQ
                     AAGVKVKKIVKTKI"
     gene            1325..1948
                     /locus_tag="PLR93_08375"
     CDS             1325..1948
                     /locus_tag="PLR93_08375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH27020.1"
                     /translation="MNIQNAYNEWSDIYDSNVNRTRDLDAEVTRLILNGQRFPSILEI
                     GCGTGKNTEFLATLGDAVLAFDFSEKMLAKAQEKVTANHVRFEQADITQPWNCEPDSV
                     DLITCNLVLEHIENLDHIFAQAARTLRSGGWFLINELHPFKQYGGTKARFERGADIIE
                     VDVFIHHISEFIRAAEKNGLKLMKFDEVWHADDAGKPPRLVSFVFGK"
     gene            complement(2005..2679)
                     /locus_tag="PLR93_08380"
     CDS             complement(2005..2679)
                     /locus_tag="PLR93_08380"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNH27021.1"
                     /translation="MPPLNPLSKREQDVVRLLLEGKSNKLIAQALRITERTVEFHLKN
                     IYAKHRVNSRMELVLHLRQSTVAGAGEDAQNGEQSTLSSWAVSTFKEAVIRIGKELKM
                     NTPTNAHVSDRDPQTFQDAIRICFVKYADFTGRASRPEFWWFALFILLSAAVLSQIDE
                     TLRAIFMIISLVPLLAVGARRLRDSGTNPWWLFGLLIPVGGIIILGFFWAAPPAKALA
                     EDSLPA"
BASE COUNT          733 a          692 c          780 g          744 t
ORIGIN      
        1 ggtctatcgc atttatccaa gctatcagct ttatcgtctc tctcatcgct ttcgggcttg
       61 tcctcgcttt ctccagccga ccctgaaagc ccgcgtaatg cccgcacccg cgaattgctt
      121 ggcgcactca aagaagccgg cgtggaccac gtcaccatga gcgatttgca agagatgaaa
      181 atccacaacc tcagcgccga ctacgtccgc gaaatgcgtg gacttggcat cgaacccgac
      241 ggcatagggg agtggatcaa cctgcgcatt cacgacattt ccccgcgcta tgtgcgtgac
      301 ctgcgcgacg tgggcgtgac cgacctcgat gtggacgaaa tcgtggactt gtacttgcaa
      361 ggcgtctctg ccaaatttat cggcggcttg cgtgacctcg gcatcaacga cctcgacatt
      421 gacgagatca aagaactgaa caaccacgat atctctccca agttcattgc cgaaatgcgc
      481 agcctcggct tcaaagacct cgacgtagat gaactggtgg aactcgccat tcacgacgtc
      541 tccccgaaat tcgtggctga aatgcgcgat cttggtttga aagacctcga tgtagacgaa
      601 ttggtagaac tcgccaatca cgatgtctct cccaaatttg tggctgaatt gcgtgccctt
      661 ggcttgaaag acctcgatgt agacgaactg atcgaatttg ctaatcacga cgtctcgccg
      721 aaatttattg ccgaattacg ttccctcggg tttactgatt tcgatgccga cgacttgatc
      781 gaacttgcca accacgatgt gactcctgat ttcatcgccg ctatgcgtga agtggggctc
      841 aaagacctgg ctgccgacga atttattgac ctgcgcaatc agggcatcac ccccggctac
      901 gtccgcgcaa tggtggaagc gggcttgaaa gacctagaag tggatcaact agaagagtta
      961 aagatccata atgtgacccc caagttcgtg cgcgaagttt tagaaatggg tttcaaagat
     1021 gtagacatcg atgaactggt agaactcagc attcaccacg tcacaccgcg cttcatgcgt
     1081 gatgtgcgtg aaaaacttgg tgaagacctc accctcgaac agattctcga tatccgcttg
     1141 cacggtatcg acgaagactt gatggaagaa ttgcaagccg ctggtgtaaa ggtcaagaag
     1201 attgtgaaga ccaaaatata gctcgctcca caataagaac attctatatc ttgctctggt
     1261 cagggtttag ttctgaccag agcattttta acgctaaaat tattgttact tgaaaggtat
     1321 tcttatgaat atacaaaacg cctataatga atggtcagat atttatgact cgaacgtcaa
     1381 ccgcacccgt gacctggatg ccgaagtgac gcgcctcatt cttaacggac agcggttccc
     1441 gtccattcta gagatcggct gcgggacggg caagaacacc gagttccttg ctacgcttgg
     1501 ggatgctgtg ttggcgtttg acttctctga aaagatgctt gccaaagctc aagagaaagt
     1561 gacagcaaat cacgtgcgct ttgagcaggc ggatatcact cagccttgga actgcgagcc
     1621 agattcagtt gacttgatca cttgtaattt agtcttggaa catattgaga atctagacca
     1681 catcttcgcc caagccgcgc ggactttgcg cagtgggggc tggtttctca tcaatgaact
     1741 gcatcccttc aagcaatacg gtggcaccaa agcccgcttc gagcgcggcg cagacattat
     1801 cgaagtggac gttttcattc accacatctc cgaattcatc cgcgctgctg aaaagaatgg
     1861 actgaagcta atgaaatttg atgaagtttg gcatgcagac gatgctggca aaccgccacg
     1921 cttggtttcg tttgtttttg gaaaataata gctgcaaaac aaaagactgc cggcaggcag
     1981 tcttttgttt tgacgttaaa aactctatgc gggcagtgaa tcttctgcca atgctttggc
     2041 gggcggagct gcccagaaga agccgaggat gatgatgcca ccgacgggga tgagcagacc
     2101 gaatagccac cagggattgg tgccgctatc gcgcaggcga cgggcaccga ccgccagcaa
     2161 agggaccagg ctgatgatca tgaagatggc gcgcagagtt tcgtcgattt gagataagac
     2221 tgcagcggag aggaggatga agagggcaaa ccaccaaaat tcagggcgtg aggcgcgacc
     2281 tgtaaaatct gcatatttga cgaagcagat gcggatcgcg tcttggaagg tttgcgggtc
     2341 tctatctgag acgtgggcgt tggtaggggt gttcattttt agctccttgc cgattcgaat
     2401 gacggcttct ttgaaagtgg atactgccca actcgataga gtggactgct ctccattttg
     2461 cgcgtcttcg cccgcgcctg caactgtgga ttgcctcagg tgcagtacca gttccatgcg
     2521 tgagttgacc cgatgtttgg cgtagatgtt tttgaggtgg aattcgacgg ttcgttcggt
     2581 gatgcggagc gcttgcgcga tcagtttgtt gcttttgcct tcgagtaata ggcgcactac
     2641 atcttgttcg cgtttgctga ggggattaag ggggggcata tccagttcct ttccacgtga
     2701 tttctaagga attttacgga gttcactttg ggatagttgc ttttcaaaag tatacccgct
     2761 gtcgttgttg gtttgagcgg gggagtgggg caggaaaagc tctctaggga ggttttaaaa
     2821 cgagtctaga gaggatttga aacgagtcta gagaggtttt aaaaacttct aaatattttt
     2881 gcaaaaacat ttagaagttt tgcggaaaac atttagaagt gttggggaaa aggtttcgaa
     2941 gtttttttt
//
LOCUS       DAOQFT010000305         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20305_length_2945_cov_8.468512, whole genome shotgun sequence.
ACCESSION   DAOQFT010000305 DAOQFT010000000
VERSION     DAOQFT010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20305_length_2945_cov_8.468512"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLR93_08385"
     CDS             <1..437
                     /locus_tag="PLR93_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNH27022.1"
                     /translation="AGEPVNSGIGFAISINIVKRVVPVLIETGNYDYPYLGVSSSSGL
                     SLDAIKALGLPQFTGAYVSSVVPGGPADRAGLKAGDQPTNFPGLMAGGDLIIAIDGRE
                     VRTFDDLLAYLITNKGPGDTVVLTVLRGTEKVDISIVLDKRP"
     gene            complement(481..1122)
                     /locus_tag="PLR93_08390"
     CDS             complement(481..1122)
                     /locus_tag="PLR93_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH27023.1"
                     /translation="MFQFEGFDRRKQEDKVREEGRLPPGQSLTQKFPVLHYGPVPTFN
                     PAAWDFRVWGEVEEEKRWSWDEFNQLPRTKLHMDIHCVTRWSKFDTDWEGVSVKTLVE
                     QGFFKIKPTATHVMQHAEYGFTVNLPLEVVLQENFLLATHFQGEPISPDHGYPLRGVV
                     GFIPGQEELETPYFWKGAKWLRSLEFMPRDKRGFWEQAGYHNRADVWREERFG"
     gene            1387..>2945
                     /locus_tag="PLR93_08395"
     CDS             1387..>2945
                     /locus_tag="PLR93_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNH27024.1"
                     /translation="MKQPTFKQRFQYWFDNYMSKGTPALLFGLFVLSAIIIFAAAALV
                     KLTNTAPNGESLLQIAWMSLLRTLDSGTMGGDTGSPFFLFMMLIVTFGGVFVVSALIG
                     ILNNGLEDKLEELRKGRSVVLENDHTLILGFTPQIFTIISELVAANESRKSGAVIVVL
                     ADDDKVDMEDAIKERIPDTKNTRIICRSGSPMDMTDLEIASPHTARSIIILADGDDPD
                     TRVIKSVLAITNNPNRRNEPYHIVTQIRDPQNMGVIKLLSEKDTVQPILTTDLIARVV
                     AQTSRQSGLSVVYTELMNFGGDEIYFKQEPGLSGKTYGDALLSFETSTVMGIRRADGT
                     IMMNPAMDTRIEPGDSIFAIAEDDDKIQLTSSRLTLEENLIQRSGNRSKPKPERGLIL
                     GWNRSGSIIIRELDNYVAKGSELLVVSDVDGLESQIQREVGKLKNQKLKVMEGDIRDR
                     ALLDELGVTEYDHVIVLAYSHLDVQDADAITLVTLLHLRDIAEKDETPFSIISEMLDL
                     RNRELAEAAKV"
BASE COUNT          742 a          761 c          683 g          759 t
ORIGIN      
        1 atgcgggaga gccggtcaac tcgggcattg ggtttgccat ctcgattaac atcgtcaaac
       61 gtgtggtgcc ggttttgatc gaaactggaa attatgatta tccatacttg ggtgtctcct
      121 ccagcagcgg acttagcctc gatgcgatca aagctttggg cttgccgcaa tttactggcg
      181 cgtatgtaag ctccgttgtc ccaggtggtc ctgcagaccg tgctgggttg aaagctggtg
      241 atcagcccac aaacttcccc ggcttaatgg caggtggtga cttgatcatt gccattgacg
      301 gtagagaagt gcgcactttc gatgacctgt tagcgtatct catcaccaac aaaggacccg
      361 gcgatactgt ggtgttgacc gttctgcgtg gaacggaaaa agtagatatt tcgattgtgt
      421 tggacaaacg accataaata aaaaaactcc gaagtcctta agacttcgga gtttttttat
      481 ttaaccaaaa cgctcttcgc gccaaacatc agcccggttg tgatacccgg cttgctccca
      541 aaatccgcgt ttatcgcgcg gcataaattc taaagaacgt agccacttgg cacccttcca
      601 aaaatagggt gtttccaatt cttcctgccc ggggatgaac cccaccacgc ctcttaatgg
      661 atacccatgg tcgggtgaga tcggctcgcc ttgaaaatga gtggcgagca ggaagttttc
      721 ttgcaatact acttccaagg gcaagttgac cgtaaagccg tattccgcat gttgcatcac
      781 gtgtgtggcg gtcggtttga tcttgaagaa accttgctcc actagagttt tcactgaaac
      841 cccttcccaa tcggtatcga acttgctcca acgggtcaca cagtggatgt ccatatgaag
      901 tttggtgcgt ggcagttgat tgaattcatc ccaactccag cgtttttctt cctccacttc
      961 gccccataca cgaaagtccc atgcggcggg gttaaatgtg gggacgggtc catagtgcaa
     1021 aacgggaaat ttctgggtca atgattgccc ggggggaaga cgcccctctt cacggacttt
     1081 atcttcctgc ttgcggcggt caaaaccttc gaattggaac atggttacct cactttacta
     1141 aagtctgcct actggcatgg aatagacatg aaagaaacgc cagatgttac cctgcaaaac
     1201 taataattgt ataagaagct agagctttga attgcccgaa atgcttttct taaattctca
     1261 gtgacctctg tcgtttaaga ccatctgcaa aatatgtaaa tcaaatgtaa tgaaaatacc
     1321 ctctggtcat agatgcaatt gctcttataa taagccaaat tttcaaaggc gcaaggagat
     1381 agttttatga agcagcccac attcaaacag agatttcaat attggttcga caactacatg
     1441 tccaaaggga cacccgcgtt attatttggc ttatttgttc tttctgccat tatcattttt
     1501 gcagccgccg ctcttgtcaa actgacaaac acagcgccca atggtgaaag cctgctccag
     1561 atcgcctgga tgagcttact gcgcaccctc gactccggca ctatgggcgg cgatactggc
     1621 agcccgttct tcctgttcat gatgctcatc gtcacgtttg gcggtgtgtt tgtggtcagc
     1681 gccctgatcg gtatcctcaa taacgggtta gaagataaac tcgaagaact tcgcaagggg
     1741 cgttctgtcg tccttgagaa tgaccacacc ctcattcttg gtttcactcc gcaaattttt
     1801 accatcatct ccgaattggt tgccgcaaat gaaagccgca agagcggtgc cgttattgtc
     1861 gtcttggcag atgatgataa agtggatatg gaagatgcca tcaaagaacg cattcctgat
     1921 accaagaaca cccgcatcat ttgccgttct ggcagcccaa tggatatgac cgatcttgag
     1981 atcgccagcc cgcataccgc gcgctctatt attatccttg ccgatggcga tgacccagat
     2041 acccgcgtta tcaaatctgt cttggcgatc accaacaacc ccaatcgtcg taatgagccg
     2101 tatcacatcg tcacccaaat ccgtgacccg caaaatatgg gtgtgatcaa actgctctct
     2161 gaaaaagata ctgtccagcc aattctcacc accgacctca ttgcccgcgt cgtagcgcaa
     2221 acttcccgcc aaagcggttt gtctgtggtc tacaccgagt tgatgaactt tggcggcgac
     2281 gaaatttact tcaagcagga acccggtctc tctggaaaaa catacggcga tgctcttctg
     2341 tcgtttgaaa cttccacagt gatgggtatt cgccgcgccg atggcaccat catgatgaac
     2401 cccgcaatgg acacccgcat tgaacccggt gactccatct tcgcgattgc tgaggatgat
     2461 gacaagattc aacttacgtc atctcgcctc actctggaag aaaacctgat tcagcgttct
     2521 ggaaaccgct ccaagcccaa gcctgagcgc ggacttatcc tcggctggaa tcgttctggt
     2581 tccatcatca ttcgcgaatt ggataactat gttgccaaag gctctgaact tttggtcgtg
     2641 tctgatgtag atggactcga aagccaaatt cagcgcgaag taggcaaact caagaatcag
     2701 aaactcaagg ttatggaagg tgacattcgc gaccgtgcct tgcttgatga actcggcgtg
     2761 accgaatatg accatgtcat cgtgttagcg tatagccatc tcgatgtcca agatgcagat
     2821 gcgatcactc tcgttactct tttgcacctg cgcgatattg ccgagaagga tgaaacgccc
     2881 ttctccatca tcagcgaaat gctcgacctg cgcaaccgcg aacttgccga agccgccaaa
     2941 gtgga
//
LOCUS       DAOQFT010000306         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20327_length_2943_cov_6.636773, whole genome shotgun sequence.
ACCESSION   DAOQFT010000306 DAOQFT010000000
VERSION     DAOQFT010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20327_length_2943_cov_6.636773"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLR93_08400"
     CDS             complement(<1..335)
                     /locus_tag="PLR93_08400"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH27025.1"
                     /translation="MADEKRKLPRRNFSYYMRVLKKDTGELIGQIIDISTGGFKLESS
                     KPLPSNIQMDLCIDQVNQISPRSYIVFSARTRWCDRDPYDPTIYNVGFQLVDMTPADY
                     DVFVKMFNA"
     gene            complement(432..1580)
                     /locus_tag="PLR93_08405"
     CDS             complement(432..1580)
                     /locus_tag="PLR93_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNH27026.1"
                     /translation="MRKVAILGIGQTKVDEHWDKSLREIGGEAAFLAMQDAGMDKVDA
                     LYVGNMLSPMVSGQNQLGAFFSDWIGLWKQEAVKIEAACGSGSAAFRSALMAVASGDV
                     ESALVVGVEKMTDKAGKDVTAALATAADADYEVEQGISFVGINALVMRRYMHEFGWKH
                     EDFAPFSINAHANAMHNPYARLHEKVSVEKFEKSSMVATPINLLDASPVGDGAAAVII
                     VPADKVMRQPKVVVAASGAATDTIAVHSRKDPMFLNASYLSSQQAYKMAGITPEDIDV
                     FELHDAFSIMAALSLEATGFAERGQGVRLGLENQIGIKGKIPVCTRGGLKARGHPVGA
                     TGMYQIVEVAQQLRGECGATQVDGAVIGMAQNIGGSGATIITHILRRE"
     gene            1711..2148
                     /locus_tag="PLR93_08410"
     CDS             1711..2148
                     /locus_tag="PLR93_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27027.1"
                     /translation="MQKYGFILILLAVLVIGCKGEEKSYASPTLSEEWTIKMTQSGGI
                     MGLRRSIQVASDGSYLIVDERAQTSVTGTLTEQQLSELTDLIANLKFISPDNRAICAD
                     CFVYDLEIESGGKKMILQVEDINLPNSGIEALVTFLRGILESA"
     gene            2156..2677
                     /locus_tag="PLR93_08415"
     CDS             2156..2677
                     /locus_tag="PLR93_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27028.1"
                     /translation="MNRLEWQEKYFQKVAESSEEGAEAVRYVREKKIRVGMRRARKSV
                     GAFWTWEHAFYLNKVHYTMESALENPRAWTLFVHEVRHLQQGIPTAMSVYGELDAWQY
                     EFRLFKKITGKTLKPELEEILSLPLNFDRANLRYAQKLMTKFGGFWYAAWILPLYPIH
                     KEILYWVTRKQFA"
BASE COUNT          802 a          721 c          649 g          771 t
ORIGIN      
        1 tatgcattga acatctttac aaagacgtcg taatctgccg gggtcatatc cacaagctga
       61 aacccgacgt tgtaaatcgt agggtcatac gggtcacggt cacaccagcg agttcgggct
      121 gaaaagacaa tataacttct cggtgagatc tggttcactt gatcgataca taagtccatt
      181 tggatattgg acggtaacgg cttgctgctt tcaagcttaa atccgcctgt actgatatca
      241 ataatctgac cgatcaattc accggtatcc tttttcagca cacgcatgta gtaagaaaaa
      301 tttctgcgcg gtaactttcg tttttcgtct gccatctcat cctccttgca aatttgatag
      361 gcgttttaat tttagtgcaa gtaccaagga gatacctgaa aatcagacga aaaattatga
      421 aattgcaatg attattcgcg tcgcaggata tgtgtgataa tcgttgcgcc agaaccacca
      481 atattttgtg ccataccaat aaccgcacca tcaacttgcg tcgctccgca ttcaccacgc
      541 aactgttgag caacttccac gatctgatac atacccgttg cccctacagg atgaccacgc
      601 gccttcaacc cgcctcgcgt gcagactggg atctttcctt tgattccaat ctgattctcc
      661 agcccgagcc gaactccctg accgcgctcc gcgaaacccg tcgcttcgag agacaatgcc
      721 gccataatgg aaaaagcgtc atgaagctca aagacgtcaa tatcttcagg cgtaatgcca
      781 gccattttgt acgcctgttg tgaagaaaga taagaagcgt tcaaaaacat tgggtctttg
      841 cgcgaatgga cggctattgt atccgtcgcg gcaccggaag cggctacaac cactttgggc
      901 tgtcgcatca ccttatcagc agggacgata atcaccgccg ccgccccatc ccccaccggg
      961 gaagcatcca acaaattgat aggtgttgcc accatagatg atttctcaaa tttctcaaca
     1021 ctcacttttt catgcaaacg tgcatacggg ttatgcatgg cattggcatg cgcattgatg
     1081 gagaacggtg caaaatcttc gtgcttccag ccaaactcgt gcatataacg tcgcatgacc
     1141 aaggcgttga taccgacaaa tgaaatccct tgttccactt cataatccgc atcggctgcg
     1201 gttgccaacg ccgcggtaac atctttaccg gctttgtcgg tcattttttc cacaccaacc
     1261 accaaagcgg actcaacatc acccgaagcg accgccatca gcgccgaacg gaatgccgcc
     1321 gaaccggagc cacaagccgc ttcgatctta accgcttcct gtttccacaa cccgatccaa
     1381 tcgctaaaaa aggcaccgag ctgattctgt ccacttacca tgggagaaag catattgccc
     1441 acatacaagg catcgacttt gtccatgccg gcatcttgca tcgcaagaaa tgcggcttct
     1501 ccgccgatct cgcgcaggga cttatcccaa tgttcatcta cttttgtctg accgatccca
     1561 agaatggcta cttttctcat aacacctcag ccctgattct atcgtattcg cctctcagga
     1621 actatctgaa tttttgagcg tctggttatt tggtaggata gccccatatc ccgtttatgc
     1681 tatcctagat aatacctaag gagaaacctc atgcagaaat atggctttat attgatccta
     1741 cttgctgtgc tggtcatagg ctgtaaagga gaagaaaaat catacgcctc cccaaccctt
     1801 tctgaagaat ggacaatcaa gatgactcaa tcaggcggaa tcatgggatt gcgccgttcc
     1861 attcaggtcg cctcagatgg aagttacttg atcgtagatg aacgtgcgca aacttcggtg
     1921 acaggtactc tgactgaaca acaattaagt gaattaacag acttaattgc gaatctaaaa
     1981 tttatttcgc ccgataatcg cgcgatctgt gctgattgct ttgtctatga ccttgagatc
     2041 gaaagcggcg gaaagaagat gatcctccaa gtagaagata tcaacctgcc caattcagga
     2101 atcgaagcat tggtcacctt cttgcgcgga attttagagt ctgcttgata cggctatgaa
     2161 tcgtttggaa tggcaggaaa aatattttca gaaagtagca gagtcttctg aagagggtgc
     2221 agaggcggta cgctatgtgc gcgagaaaaa gatccgggtt ggaatgaggc gggcgcggaa
     2281 gagtgtaggc gcattctgga cgtgggaaca tgctttttat ttgaacaagg ttcactacac
     2341 gatggagtcc gcgcttgaaa atccacgggc ttggactctc tttgtgcatg aagtgcgaca
     2401 cttgcaacaa ggaattccta cagccatgtc tgtatatggt gagttggatg cctggcaata
     2461 tgaatttcgt ttattcaaga aaattacagg gaaaacactc aaaccagaat tagaagagat
     2521 actttctttg ccacttaact ttgaccgggc gaatttaaga tatgctcaaa aattgatgac
     2581 caaatttggt ggtttttggt atgccgcatg gatattgccc ttgtacccga ttcacaagga
     2641 gattctatat tgggttacac gaaaacaatt tgcctgaaca accaatttca aaattacaaa
     2701 gtctttttaa tgtaaagcgt tattttcgtt gtactatgtg aataggaata agaaaggagg
     2761 ttgcgatcgt tacaccacct gaaatgcaaa ccgaagggaa gggcgatacg ccataagctg
     2821 gtcaagtgtg ttggttgctt cgtaccagac gttcccctaa cgaaaggaag gctgcattct
     2881 tcgcctcttc cccgacatag ttcgtctcga cggatgggta aactcccatc cgtctttttg
     2941 ttt
//
LOCUS       DAOQFT010000307         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20481_length_2931_cov_4.881085, whole genome shotgun sequence.
ACCESSION   DAOQFT010000307 DAOQFT010000000
VERSION     DAOQFT010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20481_length_2931_cov_4.881085"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="PLR93_08420"
     CDS             complement(<1..1776)
                     /locus_tag="PLR93_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27029.1"
                     /translation="MNTHPTTSQYFDFRDALHENRLLGLWRMMTDFRLSYIAATISLA
                     FSALAKTSVYLLLRFFADDVLVQGKMIGSTMTQTFLWIGLGFIGLAFIEGGGAFLSGR
                     LAAYTAEGITRRLRDFLFDHIQRLSFSYHSATPTGDLIERVTSDVDALRRFFSEQAIG
                     VGRIVLLFAINWAAILYINVKLGLVSVVTIPLILWVSLWFFKKVTKAYEDYQAQEAVL
                     STTLQENLTGVRVVKAFARQDYEMEKFEKDNWLKYIKGKFLLLMHSLFWPLSDIVLGG
                     QMLFGFIFAGFMAINKEITVGDYVAYVGLLVWLIFPIRNLGRVIVQTSTGMVSYQRLM
                     DITKQAREDLNGGKVQPTGPVRGEIEFKDVSFMYSDGTHDVIQNVSFHIKPGESIALL
                     GSTGSGKTSMVNLLPRFHDYTGGQILLDGVDLKDYPRKYLREQIGIVQQEPFLFSRSI
                     RENILYGVGRSVTQAEIEDAAKAAAVHDVIIGFPDGYNTIVGEKGVTLSGGQKQRVTI
                     ARTILKNPKILILDDSTSAVDTETEASIRQALNDLMEHRTTFIIAHRIQSVMKADQIL
                     VLDRGQVVQHGTHQELVGQFGGMYRK"
     gene            2107..2478
                     /locus_tag="PLR93_08425"
     CDS             2107..2478
                     /locus_tag="PLR93_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH27030.1"
                     /translation="MTHVITSLCLRDNGCSSVCPVESINPGNPAQGWYTYYIDPSSCI
                     DCGACVPECPFHAIYPADEIPQAYKVKGGELINMAGLNGHYEGMSHTGEVIRLDSTRA
                     LTAGEVMDLTPAIALNEKFYK"
     gene            complement(2482..>2931)
                     /locus_tag="PLR93_08430"
     CDS             complement(2482..>2931)
                     /locus_tag="PLR93_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27031.1"
                     /translation="PTIVLTPTVKYPEGANYTLFYNETSLFIINRSYERRSISGFKFQ
                     NLNADGTLGEEVFDGYRWETRNVDYLPRNFCANISIFGDEDPPYLNPVDCQQYGSIQT
                     VQTRFDRPEKILFWVPKGGTTQFVVFWLNEEVARCEITAGICEVYIP"
BASE COUNT          814 a          690 c          705 g          722 t
ORIGIN      
        1 cttgcggtac ataccgccaa actgaccgac gagctcctga tgcgttccgt gctgcacaac
       61 ctgacctcta tccagcacta ggatttgatc tgctttcatc acggattgga tgcggtgcgc
      121 aatgatgaag gtcgtgcggt gctccattag gtcgttcaat gcctggcgaa tggacgcttc
      181 cgtttccgtg tccactgccg aggttgagtc gtcaaggata agaatcttcg gatttttcaa
      241 gatcgtccgt gcaatggtca cacgttgttt ctgaccgcca gagagggtta cgcctttttc
      301 accaacgatg gtgttatagc catccgggaa tccgataatc acgtcatgta cagcagcagc
      361 ctttgcagca tcttcgatct ctgcctgtgt cacactgcga cccacaccgt acaaaatgtt
      421 ctcacggatc gaacgactga acaagaaggg ctcttgttgc acaataccga tctgctcgcg
      481 caaatattta cgtggataat ctttcaggtc cacaccatcg agcagaattt gcccgccggt
      541 gtaatcatga aagcgtggca gtaaattcac catcgatgtc ttacctgaac ctgttgaccc
      601 aagcaaagcg atcgattcgc caggcttgat atggaatgaa acattctgga tgacatcatg
      661 cgttccatca gaatacataa acgatacatc tttgaattcg atctcgcctc ttaccggacc
      721 cgtcggctgg accttgccgc cgttgaggtc ttcccgtgcc tgtttggtaa tgtccatcag
      781 acgctggtat gaaaccatac cggttgaggt ctgtacgatc acacgcccaa ggttgcggat
      841 ggggaagatc aaccaaacca gcaaacctac gtatgctaca taatcgccaa cggtgatctc
      901 tttgttgatc gccataaagc cagcaaaaat aaacccaaag agcatctgtc caccaaggac
      961 aatatccgaa agaggccaga aaagggagtg catcagcaaa aggaacttgc ctttaatgta
     1021 tttcagccag ttatcctttt caaacttctc catttcgtag tcctgccggg caaaggcttt
     1081 caccacacga accccggtca ggttctcttg caacgtagta gagaggaccg cctcttgcgc
     1141 ctgataatct tcataggctt ttgttacctt tttgaagaac cacaaggaaa cccacaaaat
     1201 aagcggaatg gtcactacag aaaccagccc cagcttcacg ttaatgtaca agatcgcagc
     1261 ccaattgatc gcaaatagca gcacgattct gccgacgccg atggcttgct ccgaaaagaa
     1321 gcgccgcaag gcatccacat ctgaagtgac gcgttcgatc aagtctccgg tgggtgtggc
     1381 gctgtggtaa gagaagctca aacgttgaat atggtcgaag agaaagtctc gtaaacggcg
     1441 cgtgatgcct tcggctgtgt aagcggctaa acgtccggaa aggaacgcgc cgccgccttc
     1501 gatgaaggcg agcccaatga atcccagccc aatccatagg aaggtttggg tcatggtaga
     1561 gccgatcatc ttaccctgta caagaacatc atctgcaaaa aagcgcagga ggaggtatac
     1621 agatgtcttt gccagggcag agaaggctag tgagatcgtt gcagcaatat aggacagacg
     1681 aaaatctgtc atcatacgcc acagaccaag caggcggttt tcatggagcg cgtctcggaa
     1741 gtcaaagtat tgggaggtgg tagggtgggt attcatggtg tttctccgaa cgtaacgaaa
     1801 aacgattaaa taaaaaggct gcggcgcgtg cgccacagcc caaaaacgac aaggaactga
     1861 aagagaccta gggacggtca acaggcgcac tccaagaagg caatttgcca actgcatcga
     1921 gagtttggat gaagattgtg ttgaacatca agagtacgct cctttctttt ggaatttgaa
     1981 ttacgttatg agtttacacc tctcgtttga aaaatgtcaa ggttaagtta cggattagaa
     2041 attaattttt ccgataactt ttataactag taagcgactg gatgaatata tcaaaaggag
     2101 cctaaaatga cccatgtaat tacaagccta tgtttgcgtg acaatggatg ctcatctgtt
     2161 tgcccagtgg agtccatcaa cccagggaat cccgctcaag gctggtacac ctactatatt
     2221 gacccgtcca gttgtattga ctgtggtgct tgtgtgccag aatgcccgtt ccatgcaatt
     2281 tacccagcgg atgaaattcc tcaggcatat aaagtaaaag gcggggagct tatcaatatg
     2341 gcaggactca atggtcatta cgaggggatg agtcatacag gcgaagtcat tcgtttagac
     2401 tctacccgtg ctttaacagc aggtgaagtc atggacttga cacctgcaat cgcattgaac
     2461 gagaaatttt ataagtaaag atcagggaat atagacttca caaatgcctg ctgtgatttc
     2521 acagcgggca acttcttcat taagccaaaa gaccacaaat tgcgttgtgc cgcctttagg
     2581 aacccaaaag aggatttttt ctgggcggtc gaagcgcgtt tgtacggttt gaatagaacc
     2641 gtattgctgg caatctacag ggttgagata aggtggatct tcatcgccaa aaatgctgat
     2701 atttgcgcag aagttacgtg gtagataatc tacattacgc gtctcccaac gatagccatc
     2761 aaaaacttct tcgcctaagg ttccatcggc attgaggttt tggaacttga acccagaaat
     2821 actgcgccgt tcatacgaac ggttgataat aaacaaactg gtctcgttat aaaacaaggt
     2881 gtagtttgcg ccttctgggt atttcactgt aggtgttaga acaatcgttg g
//
LOCUS       DAOQFT010000308         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20560_length_2924_cov_6.511328, whole genome shotgun sequence.
ACCESSION   DAOQFT010000308 DAOQFT010000000
VERSION     DAOQFT010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20560_length_2924_cov_6.511328"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PLR93_08435"
     CDS             <1..1031
                     /locus_tag="PLR93_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNH27032.1"
                     /translation="QNMTIHQLSRRDFLKLSASGALGAVLSELGVDRALAAPPTSKGL
                     AIFSGVPIYDAPVANANKVELIGKDQVIELKAEVQGDYFGNQFNTTWYEVDGGFVYSA
                     PILPVEEHYQKPVYSVPTDGQLAEVSVPYTLTYRSPFFYAKNSHRLYYESTHWVNKVI
                     VNREEKGVWYEVYNKILKETVYIPSYNMRLIPNEELTQISPEVPPEEKLLHVDIATQM
                     VTAFEGEKVVLSVRCASGQHGTDTPKGEWSTYHKGPSIHMTNQGDAVANIYNLPGVPW
                     CSFFTGSGHAFHGTYWHNDYGRLRSNGCVNLPSSVAKWVYRWTNPVVPYNVDYVNLPG
                     QGTKVIIE"
     gene            1092..1667
                     /locus_tag="PLR93_08440"
     CDS             1092..1667
                     /locus_tag="PLR93_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27033.1"
                     /translation="MNQIFQGNQYLLKRQVFALTGKFRVFDTAGRLLLFSEQKMFKLR
                     EDIRVYADEAKTQEVLAIKARQIVDFSAAYDVIDSATGQKVGAMRRKGLKSILRDEWE
                     VLDVNDNVRGVLVEDSMGLALLRRFLTNLVPQNYDMLFGETRVGDFKQNFNPFTYELN
                     IDFSMDTAGQVDRRLGIAAGILLAAVEGRQQ"
     gene            1737..2162
                     /locus_tag="PLR93_08445"
     CDS             1737..2162
                     /locus_tag="PLR93_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27034.1"
                     /translation="MPQPLFAGLIVDEDGNPVQAASIGSEPAYVIDDAGFKRHIPAEQ
                     VDRAVLNQMADMMKGSEDVLSEQTAKMLGQEDPFSKAMIANQLKNIDKQFDALMKTGF
                     PEELRAYLGMMGFKIVINYHGEVVRVEQPGTSGGDEGDE"
     gene            complement(2197..2916)
                     /locus_tag="PLR93_08450"
     CDS             complement(2197..2916)
                     /locus_tag="PLR93_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH27035.1"
                     /translation="MNKAALRGEPSYVWRDGQKRRLNMIAKFAGERLQGKVLENGCGV
                     GMYVEKMTELGATVTGLEYDFERACDAHVNSPRIVNAAGEFLPLPSSTFDLILSHEVI
                     EHVQDDRSAIAEMVRVMKVGGRAVIFCPNRGYPVETHGIFWNGKYYFGNKLFVNYLPR
                     VWRDKLAPHVRVYSRRDMQKLFEGLPVKFIERTIIFGAYDNIIARLGVFGKFIRAVLQ
                     FMEATPLKILGLSHLWVVEKI"
BASE COUNT          774 a          719 c          702 g          729 t
ORIGIN      
        1 atcaaaatat gactattcac caactctccc gccgggactt tcttaaactt tccgcctctg
       61 gcgcactcgg ggctgttctc tccgaattag gcgtggaccg tgctctagcc gctcccccca
      121 cctcaaaagg actcgccatc ttcagcggtg tgcccatcta cgatgcccct gttgcaaacg
      181 ccaacaaagt cgaattgatc ggcaaagacc aggtcattga gctgaaagct gaagtgcaag
      241 gcgactactt cggcaatcaa ttcaatacca cctggtatga agtggatggc ggttttgtct
      301 actcggcacc gatcctgcct gtggaagagc attatcaaaa acctgtttac tctgtcccca
      361 cagacggaca attggcagaa gtctctgtcc cttatacatt gacgtatcgt tccccatttt
      421 tttatgccaa aaattcgcat cgcctttatt atgaatcaac gcattgggta aataaagtga
      481 ttgtgaatcg ggaagaaaaa ggggtttggt atgaggtcta taataagatt ttaaaagaaa
      541 ccgtttacat tccttcctat aatatgcggc ttattcccaa tgaagaatta acgcaaattt
      601 cgcccgaagt tccgcccgaa gaaaaattac tgcatgttga tattgccacg caaatggtga
      661 ccgccttcga gggcgagaag gtcgttctct ccgtacgctg tgcaagtggt cagcatggaa
      721 ccgatacgcc caaaggagaa tggagcacct atcataaagg tccatccatt catatgacaa
      781 accaaggcga tgctgttgcc aacatctaca acctgcccgg ggtgccgtgg tgctccttct
      841 tcactggctc tgggcatgcc ttccatggaa cctattggca caatgattat ggtcgcctcc
      901 gcagtaatgg atgtgtcaat ttgccctcca gtgtcgcaaa atgggtctac cgttggacga
      961 acccagttgt gccttacaac gtagattatg tgaacctgcc cggtcagggc acgaaagtaa
     1021 tcattgaata ggttggcacg aacgtaaaag acgttcgata aattttttag aatcaaaaaa
     1081 aggacagaga gatgaatcaa atttttcaag gcaaccaata tttactcaaa cgccaggtct
     1141 tcgcactcac tggtaaattc cgcgtgttcg ataccgccgg cagattgctg ctcttcagcg
     1201 agcagaagat gttcaagtta cgcgaagata ttcgcgtata tgccgatgaa gccaagacac
     1261 aagaagtttt ggcgatcaaa gctcgccaga tcgtagattt ctcggctgca tatgatgtga
     1321 ttgacagtgc cacaggtcag aaagtgggag ccatgcgccg caaaggtctt aagtcgattc
     1381 tgcgcgatga atgggaagtg ctggatgtca acgacaatgt gcgcggtgtt ttggtcgaag
     1441 atagcatggg tcttgcttta ctgcgccgct tcttgaccaa tctcgtgccg cagaactacg
     1501 acatgctctt cggcgaaact cgcgtaggcg atttcaaaca gaatttcaat cccttcacgt
     1561 atgaactgaa catcgacttc agcatggata cggcaggtca ggttgaccgc cgtcttggca
     1621 ttgccgctgg cattttgctt gctgcggttg aaggtcgcca acaataattt cttgatatct
     1681 ttcctctttc ttctttcttg caaaacaaaa gaggaaagaa gaaagaggag ttcttaatgc
     1741 cccaacctct cttcgccggt ctcatcgtag atgaagacgg caaccccgta caagccgcat
     1801 ccattggcag tgaacctgct tacgtaatag atgatgccgg attcaaacgt cacatccctg
     1861 ccgagcaagt ggatcgcgcc gtgctcaacc aaatggcaga catgatgaaa ggcagtgaag
     1921 atgtcctttc ggagcagact gccaaaatgc ttgggcagga agatcccttc tcaaaggcga
     1981 tgatcgcgaa tcagctaaag aatatagata agcaattcga tgccttgatg aaaacaggct
     2041 ttcctgaaga actgcgtgcc tatttaggga tgatgggttt caagatagtt atcaattacc
     2101 acggcgaagt ggtacgagtg gaacagccgg ggacttccgg tggcgatgag ggtgatgaat
     2161 aaagaaaaac tccgaggtgt tgaacctcgg agttttttat atcttctcta ccacccaaag
     2221 atgggataaa cccaatatct ttagcggtgt cgcttccata aattgcaaca cagcacggat
     2281 aaatttcccg aagacaccca agcgggcgat gatgttatcg taggcaccaa agataatggt
     2341 gcgttcaatg aatttgacag gtaatccctc aaatagtttt tgcatatcac ggcgggaata
     2401 cacgcgcaca tgcggggcga gtttgtcgcg ccatactctg ggtaggtaat ttacaaacag
     2461 cttattacca aaataatatt tgccattcca gaaaatacca tgggtttcca cggggtatcc
     2521 gcggttgggg cagaagatga cagcccgccc acccactttc atcacgcgta ccatctcggc
     2581 aatcgcggag cggtcatcct gtacgtgttc gatcacttcg tggctgagaa tcaggtcaaa
     2641 cgtcgaagaa ggaagcggga ggaattcccc ggcggcgttg acgatacgcg gcgaattcac
     2701 atgggcatca caggcgcgtt caaagtcata ttccaacccg gtgactgttg ccccaagttc
     2761 ggtcatcttt tctacgtaca tgcccacgcc gcagccattt tccagtacct tgccttgcag
     2821 gcgttccccg gcaaattttg cgatcatatt caaacggcgc ttttgtccgt cgcgccatac
     2881 atacgaaggt tctccgcgca gggcggcttt gttcatatct cgtt
//
LOCUS       DAOQFT010000309         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20620_length_2920_cov_8.132984, whole genome shotgun sequence.
ACCESSION   DAOQFT010000309 DAOQFT010000000
VERSION     DAOQFT010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20620_length_2920_cov_8.132984"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLR93_08455"
     CDS             complement(<1..683)
                     /locus_tag="PLR93_08455"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNH27036.1"
                     /translation="MESTNEPVSQEPVVQEPAMHETPVRRRQPKPRGINPFLSTLGIA
                     VLIATLFTAWTPNSIFTNNLSDQLRTLLTPQSAIQSGASTPLPQIRIGIVAGHMGNDS
                     GAVCYDDNGEPDLTEADVNLSIATLVQRSLTERGFQVDLLNEFDTRLNGYRAAALVSI
                     HNDSCDYINDEATGFKVAASLETRDVNRAQRLTACLTDRYQNLTGLFFHANSITPDMT
                     QYHAFSEIDP"
     gene            complement(735..1706)
                     /locus_tag="PLR93_08460"
     CDS             complement(735..1706)
                     /locus_tag="PLR93_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27037.1"
                     /translation="MLSSSDFLHLPYTPDLTEAGIAYALRSLAYSYERDGRSPYGRLR
                     RLVANVAVELAFRRYLTQEHVSFEVKASTPFTDRERFDVVLNGHRCDLKPFLISHREQ
                     ILQIRKNPAVLLDAQALVPSDSHAGDGHSYNDIYLFAFMNGLVAASQADLQKAIEKNQ
                     PHYLVYVPSMLLRKPMHWKPLGRLTLRSESAEELIVEVNGQDEAREMKREVISLSPGV
                     NVKLHEVFYSITSLHVRRVPEARIRIHCESLNETLVIAPHDWSNIWVYGMDIFMVGYA
                     TYEEMSQRATLLLPNSRTFQYERTHVKNLSLLVSKLKPIQKLWTAVS"
     gene            complement(1822..2610)
                     /locus_tag="PLR93_08465"
     CDS             complement(1822..2610)
                     /locus_tag="PLR93_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27038.1"
                     /translation="MTFKRILRSGLLALLLTSCSALDEMLATNTPSPATETPAPTSTI
                     VWFPPSATPTLQFLSTKAPTPEMRPGLSNELLRDDFSSPFDWDVVTSNDASAVMQDNR
                     LTLAVQSQVYMTSLRHDTLLTDYYAEITAQPSLCKGEDSYGFLIRANGGSYYRFALAC
                     DGTVRAERVSNSVRLVLQKPIPSGDVPPGAPGNVRIGVWIVGNEMRLFLNGRFQFSIN
                     DPSFPIGTVGVFVRSAGETATIVSFSDLVIQEVDYIPPTPTATP"
     gene            2688..>2920
                     /locus_tag="PLR93_08470"
     CDS             2688..>2920
                     /locus_tag="PLR93_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH27039.1"
                     /translation="MTVKQVLITGACGEIGQALVQELAKKGGYRIVTSDMAPLPDSIK
                     NLVAEHVQGDLVYKVKVLYDYDFDVIFHLAASLS"
BASE COUNT          735 a          619 c          837 g          729 t
ORIGIN      
        1 gggtcaattt ctgagaaggc gtgatattgg gtcatatcgg gcgtgatgct gttggcatgg
       61 aagaaaagcc cggtcaggtt ttggtaacgg tctgtgaggc aggcggtgag gcgctgtgca
      121 cgatttacat cgcgagtttc aagcgaagcc gctactttga acccggtcgc ttcatcgttg
      181 atgtaatcgc aagagtcgtt atgaatggac accaatgctg ccgcacgata gccattcaaa
      241 cgtgtatcga attcattgag caagtccact tgaaagccgc gctcggtgag gctgcgctgt
      301 acgagggtcg caatcgataa gttgacatcc gcttctgtta gatcgggttc gccattgtca
      361 tcgtaacaaa cagcccccga gtcattgccc atgtgcccgg caacaatgcc aatgcgaatt
      421 tgcggcaaag gcgtggatgc gccgctttga atagctgatt gcggcgtgag caatgtgcgc
      481 agttgatcgc tgagattatt tgtaaaaata ctgttggggg tccaagcggt gaaaagagtt
      541 gcgatcaaga ccgcaatgcc cagcgtggaa agaaaagggt taatgccgcg cggtttgggc
      601 tgcctgcgtc gaactggagt ttcgtgcata gcaggctcct gaacaacagg ttcctgtgag
      661 acgggttcgt ttgtagattc catagctaaa taatattacc ttattttgct tggatacaaa
      721 tgaaaaacag attgctacga aaccgccgtc cataactttt ggatgggctt tagcttggag
      781 accagcagcg aaaggttttt gacatgcgtg cgttcgtact ggaatgtccg cgagttgggc
      841 agtaacagtg tggcgcgttg actcatctct tcgtatgtgg cgtaacccac cataaaaata
      901 tccatgccat agacccaaat atttgaccag tcatgtgggg cgattacgag tgtttcgttc
      961 aacgattcgc agtggatgcg aatccgcgct tcgggcacgc ggcggacatg caaggaagtg
     1021 atcgaataga agacctcatg caatttgaca ttgacgcccg gcgaaaggct gatgacttcg
     1081 cgcttcattt cccgcgcctc gtcttgccca ttgacttcga cgattaattc ctccgctgat
     1141 tcagaacgca gagttaacct accgagtggt ttccaatgca taggtttacg aagtaacatc
     1201 gatggcacat agactaaata atgcggctga ttcttttcga tggctttttg caagtctgct
     1261 tgtgacgctg cgacgagtcc gttcatgaag gcaaagagat agatatcgtt gtaggaatgt
     1321 ccatctccgg catgtgagtc agaaggcact aaggcttgag catctaacaa gacggcaggg
     1381 tttttacgga tttgtagaat ctgttcgcga tgggagataa gaaaaggttt gaggtcgcag
     1441 cggtgaccgt tcaagaccac gtcgaatcgt tctcggtctg taaacggtgt ggaggctttt
     1501 acttcaaatg aaacgtgttc ctgtgtgaga taacgccgaa atgccaactc tacggcaaca
     1561 tttgccacca gacggcgcaa acgcccatat ggagaacgcc catcgcgctc ataagaatat
     1621 gccagtgacc tcaatgcata ggcaatgcct gcctctgtga ggtctggcgt gtaaggaagg
     1681 tgtaggaagt cggaagacga tagcatagta tcagtgacca gtattccaag taccaatatg
     1741 tgttgtgggt atggtgttga tgtttgatgc gtttcccact actgttctaa tcgtaaatct
     1801 caaatcaaaa atcgtcaatt gctacggtgt agcagttggg gtagggggaa tgtaatccac
     1861 ttcttgaatg acaagatcag aaaacgaaac gatggtggcg gtttcacctg ccgagcggac
     1921 gaatacgccg acggtaccaa tagggaacga cgggtcgttg atgctgaatt ggaaacgtcc
     1981 gtttaggaag aggcgcattt cattgccaac gatccatact ccaatgcgaa cgttgccggg
     2041 tgcgccgggg ggcacatctc cgctggggat gggtttttgc agcacaagcc ttacgctatt
     2101 gctcacacgt tcagcacgga cggtcccatc gcaggcgagg gcgaaacgat agtaggagcc
     2161 accattggca cgaatgagaa agccatagct atcttcgcct ttgcataagc taggttgtgc
     2221 tgtgatctcg gcatagtaat cggtgagtaa cgtgtcgtgg cgcaagctgg tcatatagac
     2281 ttggctttgc acggcaagag tgagacggtt gtcttgcatc accgcactgg cgtcgttgga
     2341 tgtcaccaca tcccaatcga acgggctgga gaaatcgtca cgaagaagct cattgctgag
     2401 accggggcgc atctctgggg tgggggcttt ggtggataaa aattgaagcg taggtgttgc
     2461 agatggaggg aaccagacaa tggtcgaggt gggagccggg gtttccgtcg ctggggaggg
     2521 ggtgttggtg gcgagcatct catccaacgc agagcaggag gttaataaga gagcgagaag
     2581 ccctgaacgt aaaattcgtt tgaaagtcat tgggttttta tgacagcgtt tcgtttcaat
     2641 gtgtcgtaaa atatttatac aacattctag catatcagga gatgaagatg accgtgaagc
     2701 aagtgctgat aactggtgcg tgtggagaaa ttggtcaggc gctggtgcaa gaattggcaa
     2761 agaagggcgg ttaccgaatt gtgacatcgg acatggcgcc attgccagat tcgatcaaaa
     2821 atttagtggc agagcatgtg caaggggatc tcgtttataa agtaaaagtt ctctatgatt
     2881 atgattttga tgtgatcttc catttggcgg cgtcgctttc
//
LOCUS       DAOQFT010000310         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20712_length_2912_cov_7.081904, whole genome shotgun sequence.
ACCESSION   DAOQFT010000310 DAOQFT010000000
VERSION     DAOQFT010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20712_length_2912_cov_7.081904"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLR93_08475"
     CDS             <1..841
                     /locus_tag="PLR93_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27040.1"
                     /translation="GTFSERLSSFSQPFYLEHMMRTKAIYPILVLLCFSFACQALFPA
                     TPPPRDGVVIDNCSEFVKSIRSMQTNDTPQELIETGIKQGGEFDVNEYFNVTPNLSMQ
                     EGYALDYVIIPDSLGSFPLLAARPVDQPPYKSSSEVSVDSELTNYWKYMEVTDNEQGY
                     FEYASFLLLANQFYLVWHANYNDLGIVCDRATVDNIVANVNDGGFGVEFDREQMKQIK
                     ALQNIEPLVKLTDTTATVELVVFSNWGGFYRMTYTISRSSPHEIMDRQDENIIPYDCG
                     IMF"
     gene            1003..2658
                     /locus_tag="PLR93_08480"
     CDS             1003..2658
                     /locus_tag="PLR93_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNH27041.1"
                     /translation="MPRRSLARLYRDEFAGYSLATFRNDLVAGLNVALVALPLALAFG
                     AASGATAAAGLVTSIVGGIIIGLLAGAPYQMTGAKAVISVVLIVLVQQYGLEGMWVAL
                     FMAGLLTLGIGLFRLGRFIAFIPSPVIAGFTAGISAIIIIRQFDPLLNIHTPTTITAA
                     QKLIGYFTTPNLSPDLHSLMMGLFVMGIMILYPTSWNKRIPGSLVALVIATASNSALG
                     WSIPTIQNIPRTLFLQPNFSIAEIPWQNISDFIAPALTLTALGAVETLLTGSIGAELT
                     GKRLQVNQHLIAQGIGNMILPFFGAIPTTSGIIRTKVAIQSGGQTRLTGIIHSLILLI
                     VMFAFADYLAMIPLSALASVLMVTAIRSVDLSSVKFTFGNFFKTDMIAFTVAFVATVV
                     LSLTNAILFGTFLMGAVFLNKSASIDINIQEVDVERLKQRGIETAGKCKHVRVAFLTG
                     PLFFAATSQFSESFADLGDTHALILSMRGVPLIDSAGIEAIHKLHIRLQRNGGTLMFA
                     GIHDNAKQMMERGGLVKTIGEENFFWSSDQAIVEAERRGCPFC"
     gene            2694..>2912
                     /locus_tag="PLR93_08485"
     CDS             2694..>2912
                     /locus_tag="PLR93_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27042.1"
                     /translation="MSQQTIVRWLSVLWLLTSQVAGAALVFAPLVVLVSFQDAATAAG
                     EAGMYNLMLGLGYVLPVVFVGLGIAAWVM"
BASE COUNT          714 a          734 c          655 g          809 t
ORIGIN      
        1 aggaacattt tcagaacggc tttcgtcttt ttctcaaccc ttttatttgg agcatatgat
       61 gagaacaaag gcaatttatc caattcttgt tttgttgtgc ttttcttttg cgtgtcaggc
      121 gctctttcct gctacgccgc caccccgtga tggggttgtg atcgacaact gttcagagtt
      181 cgttaagtcg attcgcagta tgcagacaaa cgatacgcct caagagttga tcgaaacggg
      241 catcaaacaa gggggtgaat ttgatgtgaa tgaatacttc aacgtcacac caaacctttc
      301 tatgcaagag ggatatgcgc tggattatgt cattatcccc gattcattag gttcctttcc
      361 gcttttggct gctcgtccgg tagatcaacc accatataaa tcatcgagcg aagtctccgt
      421 ggattctgaa ctcacaaatt attggaagta catggaagtc acagataacg agcagggata
      481 ttttgaatac gcttccttcc tgcttcttgc caatcaattc tacctagttt ggcatgccaa
      541 ttacaacgac ttgggtatag tctgcgatcg tgcaaccgtt gataatattg ttgcaaacgt
      601 caacgacgga gggttcggcg tggagttcga tagagaacaa atgaaacaga tcaaggctct
      661 gcaaaatatc gaaccactcg tcaagctgac ggacacgact gccaccgtag aattggttgt
      721 gtttagcaat tggggtggct tttatcgcat gacctatacg attagcagat cgtctccgca
      781 tgagattatg gataggcaag atgagaatat tattccgtat gactgtggaa tcatgttcta
      841 gaacaaaata gactacacgg attcattacg aataccgtgt agtctattaa atccgtatac
      901 aaaataaaat cagcagtgat aaaataccgc ccgacataaa ccaaaggacg ccttggggga
      961 gcaccgagcc tttggtgtac tactatttac ggagaattaa atatgccaag acgttccctt
     1021 gcccgtcttt acagggacga gtttgcgggc tattcgctcg ccacatttcg caatgacctc
     1081 gttgcaggtc taaatgttgc gctggtcgct ttgccgcttg cgcttgcctt tggtgctgcc
     1141 tcaggggcaa ccgccgccgc cggattagtc acttccatag ttggcggcat catcattgga
     1201 cttctggcag gtgcgcccta tcaaatgacc ggcgcaaaag ccgttatcag tgtggtgttg
     1261 atcgtactcg ttcaacaata tggtcttgaa ggaatgtggg tagcgctctt catggcaggt
     1321 ttgctcacac taggcatcgg tctatttagg ctcgggcgtt tcatcgcttt tatcccatcc
     1381 cctgttattg ctggttttac tgcagggatt tccgccatca ttattatccg tcaattcgac
     1441 ccgctgctca acatccatac ccctaccaca atcacagccg cacaaaaact gatcggatat
     1501 ttcaccactc ccaatctctc cccagacctt catagcctta tgatgggttt atttgtgatg
     1561 ggaatcatga tcctctaccc taccagctgg aacaaacgga ttcccggttc gctggtcgca
     1621 ttagtgattg ccaccgcctc caactccgct ctgggctggt ccatccccac catccaaaac
     1681 atcccccgaa ccttattcct gcaaccaaat ttcagcatcg cagaaattcc atggcaaaac
     1741 atttcagatt tcatagcgcc ggctttaact ctcactgccc ttggtgctgt tgaaaccctc
     1801 cttaccggat ccattggcgc cgaactgaca ggcaaacgac tgcaagtcaa tcaacatctg
     1861 attgcccaag gtattgggaa tatgatcctg ccattctttg gtgccattcc caccacatct
     1921 ggaatcattc gcaccaaagt tgccattcaa tctggcggac agacgcgcct gacaggcatt
     1981 atccactctt tgatactgct gatcgtcatg ttcgcttttg ccgattatct tgccatgatt
     2041 ccactctctg cgctggctag tgtcttgatg gtcactgcca tccgctcagt agacttaagc
     2101 tccgtcaaat ttacgtttgg caatttcttc aaaactgaca tgattgcttt cacagttgcc
     2161 tttgtcgcca ccgttgtcct gagcttaacg aacgccatcc tctttggaac ctttctaatg
     2221 ggagccgtct tcctcaacaa aagcgccagt atcgacatca atattcaaga agtggatgta
     2281 gaacgcctca agcaaagagg cattgaaacc gctggcaagt gtaaacacgt ccgagtcgcc
     2341 tttctaaccg gtccgctatt ttttgcggca acctcacaat tcagcgaatc ctttgcagac
     2401 ttaggtgaca cgcatgcttt gattctgtcc atgcgcgggg tgcctctcat cgactcggca
     2461 ggcatcgaag ccattcacaa gttacatatc cgcttgcaga ggaatggcgg gaccctcatg
     2521 tttgcaggca ttcatgacaa cgccaagcaa atgatggagc gcggtggact tgtaaaaacc
     2581 atcggcgaag aaaacttctt ctggtccagc gaccaagcca ttgttgaggc agagaggcgc
     2641 gggtgtccat tctgttgata tataatggaa atacactctc aaggagacat tccatgtcac
     2701 aacaaacaat cgttcgatgg ttaagcgttt tatggttgct aacttctcag gttgccggag
     2761 ccgcacttgt ttttgctccg ctcgttgtat tagtgagctt tcaagatgcc gctacagcag
     2821 caggtgaagc tggcatgtat aacttaatgc tggggttggg atatgttctg cctgtagtgt
     2881 ttgtagggct aggcattgcc gcatgggtca tg
//
LOCUS       DAOQFT010000311         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20833_length_2902_cov_8.555321, whole genome shotgun sequence.
ACCESSION   DAOQFT010000311 DAOQFT010000000
VERSION     DAOQFT010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20833_length_2902_cov_8.555321"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLR93_08490"
     CDS             complement(<1..217)
                     /locus_tag="PLR93_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27043.1"
                     /translation="MPQDFSSLRINPDRMLDAFTQLAAIGSTGDGGVNRTTFSEAHLH
                     ARKWFREHIQQSGLEFRTDGAGNHSAVL"
     gene            complement(299..742)
                     /locus_tag="PLR93_08495"
     CDS             complement(299..742)
                     /locus_tag="PLR93_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH27044.1"
                     /translation="MSNLIRWEPAREMMTLREAMDRLFDDAFTRPLSLSGNSWSIPAV
                     DMYQTDNEVVVKAALPGIKADEVQLNVTGEVLTIKGETKQENETKEKAYHIREQRWGT
                     FERSLVLPTEVVADKAKADFENGILTITLPKAEEVKPKTINIKTK"
     gene            complement(971..1687)
                     /locus_tag="PLR93_08500"
     CDS             complement(971..1687)
                     /locus_tag="PLR93_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH27045.1"
                     /translation="MTEMDRRRILVVDDEERMVRFIRMNLEHDGFQVGEAFNGKQAIQ
                     KIRDFTPDLILLDVMMPDIDGFEVLETIRDFSNVPVIMLTAKGEEDDRVRGLEHGADD
                     YITKPFSPRELVSRIKAVLRRTEGTGGSMHGLIEIDKRLKIDFERREIWLEGKLVKLR
                     PTEYRLLYHLVQNAGWVVSHDQLLQKVWGYEYRDEPHYVRLYINYLRQKLEKDPANPV
                     YILTERGVGYRFVDFKRSKE"
     gene            complement(1692..1934)
                     /locus_tag="PLR93_08505"
     CDS             complement(1692..1934)
                     /locus_tag="PLR93_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27046.1"
                     /translation="MAYTVILHIANEASVAGEVDELPKPTDTIIVVANPRQKDGKDLH
                     YIDQGTVKVIWPLAKVSFVEILGDSEEEKIIGFVRE"
     gene            complement(1956..2462)
                     /locus_tag="PLR93_08510"
     CDS             complement(1956..2462)
                     /locus_tag="PLR93_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNH27047.1"
                     /translation="MIICSNCKHANMTGAMFCVECGAQLIGRDTLTTQNIATDSLGRA
                     SKRKTGELSQPIEGVDAWANLQLLDTGQVLPLSSRNEFTLGRISEGQPIMPDIDLSPY
                     QAYAAGVSRLHAVIKRSGSYVKFMDLGSANGTFLNGIRLSPNVEQSIQHGDIVSLGKM
                     RIQILIEK"
     gene            complement(2459..>2902)
                     /locus_tag="PLR93_08515"
     CDS             complement(2459..>2902)
                     /locus_tag="PLR93_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27048.1"
                     /translation="LLEFVVSPEALKGEKVLVLDGLLKASIASNPIPASPIRLKLTRP
                     LEQSPSAEPPPTRVLKALSRLTLYRMQERAQSAAQNGQFDVAARHLQNLATHLLSQGE
                     HAVAKTALLEAQSLENTRTWSESGSKDIKYKTRALLLDSGKEKKS"
BASE COUNT          723 a          739 c          649 g          791 t
ORIGIN      
        1 gcaaaactgc ggagtgattc cctgccccat cggtgcgaaa ttccaatccg ctttgctgga
       61 tgtgttcgcg aaaccatttg cgggcgtgaa gatgcgcctc gctgaacgta gtgcgattta
      121 cgccaccatc accggtcgag ccgatggcgg ctaattgagt gaaagcatcc agcatccggt
      181 ctgggttgat gcgaagggaa gaaaaatctt gaggcatttg ggaaaatctc ctatattctg
      241 cctatcataa caaaaacagg agccgttaga acccggctcc tgtttcgtac gcgaaatatt
      301 acttggtctt gatattgatc gttttgggct tgacctcttc cgctttgggg agcgtgatgg
      361 tcagaatgcc gttctcaaag tccgctttgg ctttatccgc tacaacttcg gtgggcagaa
      421 cgagcgagcg ttcgaacgta ccccagcgct gttcgcgaat gtgatacgcc ttctctttgg
      481 tttcgttctc ctgcttggtc tcacctttaa tagtaagtac ctcaccggtc acattgagtt
      541 gaacttcatc ggctttgatg ccgggcaaag cagccttcac cacaacttcg ttatctgttt
      601 ggtacatatc cacagccggt atagaccacg aatttccgga aaggctgagc gggcgggtaa
      661 acgcatcgtc gaagaggcga tccatggctt cacgcagggt catcatctcg cgagcgggtt
      721 cccaacgaat taagtttgac atagctacct ccttgtagga tttgaatttt gtcttacatc
      781 ccttaattta aaacgcctgc gttagaaaaa cgcaagcgtt ttgttttcgt tttataagag
      841 aagcagttca ggggctgaag gtggtgaaaa gcacaaccaa tccgtaaatc acaccaatgc
      901 aggtcacaca aacgccaacc agctttgccg ccgtcaccca cttgttctca ggtttttgct
      961 ccgtcatggg ttactcctta ctgcgtttga aatcgacaaa acggtagcca actccgcgct
     1021 cagtaaggat gtaaaccggg ttagccggat ccttctccaa cttttggcgc agatagttaa
     1081 tatacaaacg gacgtagtgc ggttcatcgc ggtattcata gccccaaact ttctggagca
     1141 attggtcgtg agaaaccacc caacctgcat tctgcacaag atgatacagc aatcgatatt
     1201 ccgtcgggcg aagctttacc agcttgcctt cgagccaaat ctcacggcgt tcaaaatcga
     1261 tcttcaagcg tttatcgatc tcgatcagtc catgcatgga gccgcctgtg ccttcggtgc
     1321 ggcgcagtac ggctttaata cggctgacca attcacgcgg gctgaacggt ttggtaatgt
     1381 aatcgtccgc gccatgttcg agtccgcgca cacggtcatc ctcttcacct ttggcggtga
     1441 gcataatgac cggcacatta ctaaaatcgc ggatggtctc aagcacttcg aagccgtcaa
     1501 tatccggcat catcacatca agcaaaataa ggtcgggagt gaaatcacga atcttttgga
     1561 ttgcttgctt gccgttaaac gcctccccta cctgaaaacc atcatgttcc aggttcatac
     1621 gaataaagcg caccatacgc tcttcatcat ccacaaccag gatgcgacgt cgatccattt
     1681 ctgtcatacg tctattccct cacaaaacca atgatcttct cttcttcaga gtcaccaagg
     1741 atttcgacaa aacttacttt cgccaaaggc caaatcacct taacggttcc ttgatcgata
     1801 tagtgcaagt ctttgccatc tttctggcgg ggattggcaa caacaataat cgtatcggtt
     1861 ggtttaggaa gctcatccac ttcaccagcc acagatgctt cattagcaat atgcaaaata
     1921 acggtgtaag ccatatccgt tgtctctcct tcgttctatt tctcaattaa tatttgaatt
     1981 ctcatcttac ccaatgacac aatatcaccg tgctgaatcg attgctccac gtttggtgat
     2041 aaacgaatgc catttagaaa ggttccgttt gcagaaccca agtccataaa tttgacataa
     2101 gagccgcttc ttttgatgac agcatgtaag cgtgaaaccc cagcagcata tgcctgataa
     2161 ggagagagat caatatccgg cataattggt tgcccctcac taattctccc taacgtgaat
     2221 tcatttcgag aagagagagg taacacttgc ccagtatcta aaagttgtag attcgcccaa
     2281 gcatcaacac cttctattgg ctggctcaat tcgccggttt ttcgtttgct tgctcttcca
     2341 aggctatctg tcgcaatatt ctgggtggtt agtgtgtctc ttccgatcaa ctgggcgcca
     2401 cattccacac agaacatggc acctgtcata tttgcatgtt tgcaattgga acagattatc
     2461 atgatttttt ctccttgcca ctgtccaata acagcgctcg tgttttatat tttatatcct
     2521 tgctcccaga ttcgctccag gtacgcgtat tttcaagact ttgtgcttca agcaatgcag
     2581 tttttgcaac agcatgttcg ccttgtgaaa gcaaatgcgt tgctaaattt tgcaaatgcc
     2641 tggctgctac atcaaactgt ccattctggg cagcagattg cgcccgttct tgcatgcgat
     2701 acagagtaag gcgggaaagc gccttcaaaa cccgcgtcgg cggcggctct gctgagggac
     2761 tttgctccaa gggacgggtc agtttgaggc ggatgggaga tgcagggata ggattggaag
     2821 caatggatgc ttttaggagt ccatccaata ctaagacctt ctcccctttc aatgcctcgg
     2881 ggctgacaac aaattccaac aa
//
LOCUS       DAOQFT010000312         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20901_length_2897_cov_4.632653, whole genome shotgun sequence.
ACCESSION   DAOQFT010000312 DAOQFT010000000
VERSION     DAOQFT010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20901_length_2897_cov_4.632653"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLR93_08520"
     CDS             <1..247
                     /locus_tag="PLR93_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27049.1"
                     /translation="SLPVFCPLKLLCGRAAELLRYPLVGGVDKCPAGESASGVDNDGS
                     GAESHKSGARFVRWRDSLCDNRPSKMSIIAILCLTAF"
     gene            complement(388..615)
                     /locus_tag="PLR93_08525"
     CDS             complement(388..615)
                     /locus_tag="PLR93_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27050.1"
                     /translation="MSIANQVIEKAHGKAKPFELLLLKNGSIVWSEKHQTHHLAWASG
                     ENWKLLNQENTYAIREAAQQGRAVDAPQAGA"
     gene            complement(654..815)
                     /locus_tag="PLR93_08530"
     CDS             complement(654..815)
                     /locus_tag="PLR93_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27051.1"
                     /translation="MDTLTETMERILEITEITDNIRAAVASGELTKEQGKEVLDSVLD
                     VVIHLPYKT"
     gene            complement(815..1825)
                     /locus_tag="PLR93_08535"
     CDS             complement(815..1825)
                     /locus_tag="PLR93_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HNH27052.1"
                     /translation="MNKFNVEEILNNADLRDLVQKAGGVVDGHGRCACPLHGGLNDTA
                     FSIFQKEGREVWNCFTGDCGGGDAISFVMKWQGLDFKGACAFLGGDIASDPIAIEQSA
                     KARLERAKEEHEKTRLIVEARRQELQVAQMHRHYHETMKEWGRLAWVGRGIDESYQGL
                     WTLGACDDKCVNYKGEFYHTPSLTIPLVNDKYELLHIMHRLINPPNPKDRYRGEKEGL
                     GSFPNFYAFPDMQLNADLIWIIEGAIKSMVTATISPDAGWQFVGVTNQHGFDKLETDL
                     FKGKKVIVVPDPGFEQKAWSFAKRTGARFLMTPDKIDDLINANGYDGDWLAAMSKQAR
                     RP"
     gene            complement(1818..2714)
                     /locus_tag="PLR93_08540"
     CDS             complement(1818..2714)
                     /locus_tag="PLR93_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNH27053.1"
                     /translation="MYIPKMEYSAKDKKGFMPVNRGELIAVIGRPGNAKTGFMFRWAR
                     ERAKHLAQIKSSDVVLYWTMEQLIEELRLFHVAAEMNVSASDMASGQYQEWDGVKNTL
                     RNLHTTPLWLAGKSRARRKDKIKLTEAALIESLEAVEKWQDENILQGIDSIYIDYLQR
                     FRSDGRDWTQFYGDVVNGLKEAAGDFSTRVILGVQAKREVDKYDVPIPAGEDGQWTSG
                     IEQQADGTITLCRPSHYKNKGETFDGVTVTSHKHLIVNVAKRKMGPENFHEWVDFAPE
                     YNKMDNVEIRQYNIRKERGEDE"
BASE COUNT          645 a          771 c          614 g          867 t
ORIGIN      
        1 atcattgcct gtgttttgtc cattgaaact cctttgcgga cgtgccgccg aacttttgcg
       61 ttacccgctt gtgggcgggg tggataaatg cccagcgggt gaatccgctt cgggcgtgga
      121 taatgatggg agcggcgcag aatcccacaa gtcgggtgca cgctttgtta ggtggcggga
      181 tagtctttgc gataatcggc caagcaagat gagcataatt gcaattctgt gcctgacggc
      241 tttctgacga tatgcacatc ctcatcacct acgcaatgac caacgcagtc ccaaccttca
      301 caataacctt ttatgtagta actttgattt gccatgtagc acctaacgga ctgggcgtta
      361 gtggtgcgcc gcccgtgacc tgtccattta tgcgcccgcc tgcggcgcat ccactgcacg
      421 cccttgttgg gcggcttcac ggattgcata agtgttttct tggttgagca acttccaatt
      481 ttcaccagac gcccaagcca agtgatgtgt ttggtgtttt tcagaccaga cgattgagcc
      541 atttttcaaa agcaataact caaacggctt tgccttacca tgcgcttttt cgattacctg
      601 atttgcaatt gacattgttt gctcactttc ttgttgtgga cgttgccgcc caactatgtt
      661 ttatacggca agtgtatcac aacatccagc acactgtcaa gcacctcttt cccctgttcc
      721 ttggtcaact ctccagaggc gacagcggcg cggatgttat cggttatctc ggtaatctct
      781 agaatgcgtt ccattgtttc ggttagggtg tccattatgg tctccttgct tgtttgctca
      841 ttgcggccag ccagtcaccg tcatagccgt tcgcgtttat caggtcatct attttatcgg
      901 gtgtcatcaa aaaccttgcg cctgtccgct tcgcaaagct ccaagccttt tgctcaaatc
      961 cagggtcagg cactacgatt acctttttac ctttgaataa atccgtttca agtttatcaa
     1021 atccgtgttg attcgtaact ccaacaaact gccaacccgc atcgggggag attgtcgcag
     1081 ttaccattga ctttatagca ccctcgataa tccagattag atcagcattc aactgcatat
     1141 caggaaaggc gtaaaaattc gggaatgagc caagcccttc tttctcacct ctgtatctgt
     1201 cttttggatt agggggattt ataagcctgt gcataatatg aagcaactca tatttatcat
     1261 ttaccagcgg aattgtcagg ctaggcgtgt gataaaactc gcctttgtaa ttcacgcatt
     1321 tatcatcaca tgcgcccaac gtccacaagc cttgataact ttcatcaatt ccacgaccaa
     1381 cccaagccag ccgtccccat tccttcatcg tctcgtggta gtgcctgtgc atttgtgcaa
     1441 cctgcaattc ttgacgcctt gcctctacaa tcaggcgggt tttttcgtgt tcctcttttg
     1501 cccgttctag tctcgccttt gcgctttgct cgattgctat tgggtcacta gcaatatcac
     1561 cacccaagaa ggcgcacgcc cccttgaagt ctagcccctg ccacttcatc acaaaagaga
     1621 tagcatcccc tccgccacaa tcaccagtaa aacaattcca aacctcgcgg ccttcttttt
     1681 ggaagattga aaacgctgta tcattcaggc cgccgtgtaa tgggcaagca catcgcccat
     1741 gaccgtcaac cactccgcct gccttttgta caaggtctcg taaatctgcg ttattcaaaa
     1801 tttcttcaac attgaattta ttcatcttcg ccgcgctcct tcctgatatt gtattgtctg
     1861 atttctacgt tatccatctt attgtattct ggcgcaaagt caacccattc gtgaaagttc
     1921 tcaggcccca ttttacgctt tgccacattg acaatcaaat gtttgtgact ggtgactgtt
     1981 acaccgtcaa acgtttcgcc tttgttttta tagtgggaag ggcggcatag ggtaatcgtt
     2041 ccatcggctt gctgttcaat accgcttgtc cattgcccat cttcgccagc ggggataggt
     2101 acatcgtatt tatcaacctc ccttttagct tgtacgccca aaataacacg agttgagaaa
     2161 tcgccagcgg cttctttgag accgttcacc acatcgccgt aaaactgtgt ccagtctcta
     2221 ccgtctgatc ggaagcgttg aagatagtca atgtagatac tatcaatccc ctgcaagata
     2281 ttttcatcct gccacttttc gaccgcttca agtgattcaa tcagggcggc ttctgtcaac
     2341 ttgattttat ccttacgtct tgcgcgtgac ttgccagcca gccaaagggg ggtggtatgt
     2401 aggtttctta gggtgttctt tactccgtcc cattcctgat actgaccgct tgccatatcg
     2461 gaggcagata cattcatttc agcggccaca tgaaataggc ggagttcttc gataagttgt
     2521 tccatcgtcc agtataaaac cacatcactg ctttttatct gtgccaagtg ctttgcgcgt
     2581 tctctcgccc atcgaaacat aaaaccagtc tttgcgtttc ctgggcggcc gatgaccgct
     2641 attagttcgc cgcgattcac aggcataaaa cctttcttgt cttttgcgct gtattccatt
     2701 ttcgggatat acaaaggcat cccgtcaccg tctgagtctt tgagttcgtc tacatatttt
     2761 tcggttgcgt tgctggattc aattggggta aaaacaaggc ggctcataac tcacgctcca
     2821 agcgttctga tctgtgggag gattgcacac tctttacaaa cgcgctaggc cacgtctgct
     2881 taactaaaag cggcttc
//
LOCUS       DAOQFT010000313         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_20940_length_2894_cov_3.798873, whole genome shotgun sequence.
ACCESSION   DAOQFT010000313 DAOQFT010000000
VERSION     DAOQFT010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20940_length_2894_cov_3.798873"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2014)
                     /locus_tag="PLR93_08545"
     CDS             complement(<1..2014)
                     /locus_tag="PLR93_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27054.1"
                     /translation="MKRSSIDFGKLIAITIAAWILVEACVEFYNVAWGTGKAVGLFSP
                     RWLILFVSFVLLCVFVYVCVGIAVLNRERFFALIQNIVVLRNRIPILSWILAILFLTF
                     PAWFLQRTMWGVVFDGIYFRLFLWASTLFLVSFLITRGTTLVEWKSFLVALILTSSVF
                     TIAISLQGVTDYPFSMGWSEGNRIWDYSMRFGRDRYGIADNQDIYVLLDSGRMLIGGL
                     PFLIPNVTIEMVRLWIGLTLILPYFLVGIAAYIVAEKNIRIWMLMTLWVFLFLRQGPI
                     HSPLVLAAALTIFIWRKPLWLAIPLIIYAGYFAQASRFTWLFAPGLWIGMLEFAGASL
                     SNGKLHRTHWVRTITLGLSGAFGGFILPKLMLLLHVARTSSTPIDVTEQIANTGVNSE
                     FVTYAVTDQPLLWYRLLPNSTYGTGILLGLVIAVLPIILWLVWLSASKKWQLNVWQKL
                     AIVGPLLAFLGVGLVASTKIGGGGDLHNMDMFLIGVAFTVFIAWVNGGKEVVVNNEMP
                     SWVRLTLVAALVFPAVTPLRQLRSYNHGDDLTTLFVLTDKTPVEAKSFDMLPNSATVD
                     TALTEIQDAVDEYQTLGDVLFIDQRQLLTFDYIHEEKFIPEYEKKVLMNEALSTNYEY
                     YSSFYRDITNQRFSLIITEPLRTPLKDSSFEFGEENNAWVEWVSIPV"
     gene            complement(2039..>2894)
                     /locus_tag="PLR93_08550"
     CDS             complement(2039..>2894)
                     /locus_tag="PLR93_08550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH27055.1"
                     /translation="THDSNVLGLPLAKLAGIPTRVGTHLGEIRGMSKWRERFHTFLVN
                     HGVIQTLVASSARTKNNAIEVGVDPQKIITIYNAIMPFDVSHIDREAVRQKLGLQKED
                     VFFVAVGRLVYEKGHEFLVEAMSTVAKSESHAIAGICGAGPLHEALQAQIERLNLQDS
                     VKLLGQWDSIPELLAASDVFVLPSRWEGLPMALLEGMMAGLPVIATRVEGVDEVVQPG
                     EHGLLVPLESPAELAQAILQLLRSPQDRQRMGAAARERVLSSYTTDRMCEAYLQVIEQ
                     GLGVGKAG"
BASE COUNT          927 a          708 c          587 g          672 t
ORIGIN      
        1 gtacaggaat ggatacccat tcaacccaag cattgttctc ttcgccaaat tcaaaggagc
       61 tatctttgag aggggttcgc aagggttcag taataattag tgaaaagcgc tgattggtaa
      121 tatctctgta gaaagacgaa taatattcgt agttcgtact tagcgcctca ttcataagaa
      181 ctttcttttc atactcaggg ataaacttct cttcatgaat atagtcaaat gtcaacaatt
      241 ggcgctggtc tatgaataaa acatctccta gggtttgata ctcatccacc gcatcttgaa
      301 tttcggtcaa agcggtatca actgtagcac tattaggcaa catatcaaaa gatttggctt
      361 ccaccggggt tttatcggtc aggacgaata aggtagttaa gtcatcgcca tgattgtatg
      421 aacgcaactg gcggaggggt gttacagcgg ggaaaaccaa tgcagcaaca agtgtaagcc
      481 gcacccagct tggcatttca ttgttcacaa ccacctcttt accgccattg acccaagcta
      541 taaaaactgt aaaagcaacg cctatcagaa acatatccat attatgtagg tcgccaccgc
      601 caccgatctt tgtgctggca accaagccta ctccaaggaa ggcaaggaga ggtccaacaa
      661 tagcaagttt ttgccaaaca tttaattgcc actttttaga ggcagaaagc cagaccaacc
      721 aaagaataat cggcaatacg gcaatgacca acccaagcaa aatgccagtt ccataggttg
      781 agttaggcag taggcgatac cataagaggg gttggtctgt cacagcatag gttacaaact
      841 ctgaattgac gccagtattg gcaatttgtt ctgtaacatc gattggtgtt gaagaagttc
      901 gtgccacatg cagaagtaac atcaattttg gcaaaatgaa tccgccaaat gcgccggaca
      961 agcctaaagt gatagtccga acccaatggg ttcgatgcag ttttccatta gacaaagaag
     1021 cgcctgcgaa ttcaagcatt ccgatccata gaccgggagc gaacaaccag gtaaatcgac
     1081 tcgcttgggc aaaatatcca gcataaataa tcaatggaat tgcaagccac aatggtttac
     1141 gccagatgaa gatagttaat gccgcagcaa gaactaacgg agaatggatg ggaccctgcc
     1201 gcagaaacag aaaaacccat aaagtcatca acatccaaat acgaatattt ttttcggcaa
     1261 caatataagc agcaattcca actaaaaaat agggcaagat caaagtaagc ccaatccata
     1321 aacgcaccat ttcaatagtc acattgggaa tcaaaaatgg caaaccgcct attagcatac
     1381 gaccagaatc aagcaaaaca taaatatcct gattgtcagc aatgccataa cgatcacgcc
     1441 cgaaacgcat tgaataatcc cagatgcgat tgccttcaga ccagcccata gagaatggat
     1501 aatccgttac accttgcaaa gaaattgcaa ttgtaaaaac acttgaagtg agtatcaatg
     1561 caacaaggaa ggatttccat tcaaccaatg tggttccacg cgtaatcaga aaagaaacaa
     1621 gaaaaagcgt tgatgcccat aaaaacaaac gaaaataaat gccatcaaaa acaacacccc
     1681 acatggtgcg ttgcaaaaac cacgctggaa aagtcaaaaa taaaattgca agaatccagc
     1741 ttaatattgg tattctattt cttaatacaa ctatgttttg aattaaagca aaaaatcttt
     1801 cgcggttaag aacagcaatg cccacacata catatacaaa cacgcatagt aaaacaaaag
     1861 aaacaaaaag gataagccaa cgtggcgaga ataaccccac cgccttgcca gtaccccacg
     1921 ccacattata gaactcgaca catgcctcaa cgagtatcca tgcagcgatt gtaattgcaa
     1981 ttagttttcc aaaatcgata gacgaacgct tcatccttaa gcctctgctt gattacgctt
     2041 acccggcttt tcccacaccc aagccttgtt cgatcacctg caaatacgct tcacacatgc
     2101 gatcggtagt gtaagagctt aatactctct ctctcgccgc cgctcccatg cgctgacggt
     2161 cttgaggtga gcggagcaat tgtaatatag cctgcgccag ttctgctgga gactcaagcg
     2221 gcactaatag tccatgttca cctggctgga caacttcatc tacaccttcc actcgtgtag
     2281 caatgacagg cagccctgcc atcatgccct caagcaatgc cattgggagc ccctcccaac
     2341 gtgatggcaa aacaaacaca tccgatgcgg caagtaattc ggggatagag tcccattgcc
     2401 ccagcagctt caccgaatct tgtagattta atctctcaat ttgcgcttgc aacgcttcat
     2461 gcaatggacc tgcgccacag attccagcaa tcgcatgcga ttcagacttc gccactgtgg
     2521 acattgcctc aaccagaaac tcatgtccct tttcatagac gagccgccca actgccacga
     2581 agaagacatc ctccttttgc aaaccaagtt tctggcggac agcttcacgg tcaatatgcg
     2641 acacgtcaaa cggcatgatc gcattgtaaa tagtgatgat cttttgaggg tcaacgccca
     2701 cttcaatggc attattcttt gtacgcgccg aagaagcgac caacgtttgg atcactccgt
     2761 gattgacgag aaaggtatga aatctctcgc gccacttgga cattccgcga atctcgccaa
     2821 ggtgtgtgcc cacgcgagtg ggaatccctg ctaatttggc gagaggaagt ccaagtacgt
     2881 tgctgtcatg cgtg
//
LOCUS       DAOQFT010000314         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21094_length_2881_cov_5.588464, whole genome shotgun sequence.
ACCESSION   DAOQFT010000314 DAOQFT010000000
VERSION     DAOQFT010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21094_length_2881_cov_5.588464"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLR93_08555"
     CDS             complement(<1..467)
                     /locus_tag="PLR93_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27056.1"
                     /translation="MSSEERRKILQMVADGKISAEEAATLMRALDDDAEAETASAEPE
                     VIEVGPGFGGERSEAPELETVRRRAGRFSGAFLWIGILFTVLSAWAMFGIQQNAGLNF
                     WFYCMSMPLMLGIVLIALGAKSKTSRWLYVNVDRTQSKDNDGPRKISLAFPLPL"
     gene            complement(467..1573)
                     /locus_tag="PLR93_08560"
     CDS             complement(467..1573)
                     /locus_tag="PLR93_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27057.1"
                     /translation="MSRTISAGRTPKIEIQSINGDLSLVGWEGDDILIKTDEEDVNIS
                     QSGDEVKIACNDDLSLRVPKGAYVIIHSINGDASIRGVMGGIELKEIEGDLSIRDANT
                     VAIGTVHADFSLRGAKGSLSVRKADGDVSVRDVDGSVSLDSVTDDLALRDVRGNVTAN
                     VGEDVVLYLNPQSGNAYAVNAGDDILLVMPPRANAMLTLSADEIDFDWDGVENDEDST
                     SRVVTLGDGSATIALSAGGDIRVSNQSDAGESAEEFGNFAGIGMDWSGFGDRISRRVD
                     QVAARAQRKADEAIRRAEQTAREAERKASRRGGKAVLSVGRWNWDISPKGVPMPSKQQ
                     ASEEERLAILKMLQEKKITAEQAEKLLSALEGGS"
     gene            complement(1577..1957)
                     /locus_tag="PLR93_08565"
     CDS             complement(1577..1957)
                     /locus_tag="PLR93_08565"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNH27058.1"
                     /translation="MRPAPTRCPICQSEMEVVRLHCNGCDTSMEGHFALGQFSSLSPE
                     QMEFVFTFVRCEGKINRMEQELSLSYPTIRNRLHEVIRALGYEPGREETPEVDDNTRS
                     NVIADLEAGKITADQAMRMLRGEE"
     gene            2198..>2881
                     /locus_tag="PLR93_08570"
     CDS             2198..>2881
                     /locus_tag="PLR93_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNH27059.1"
                     /translation="MIRSLYYKPGQSVRTNIPPAEYPRLIRDKKGMLWVDFVAEPAES
                     AEPILRSFGFHPLAIDDALQETHTPKVDDWGDYLYIVLSIMNYREESGMFDSEIDELD
                     VFLGSNYVITFHDQLLTAIEDAWVACQRDIRHVQEGADHLLYRIVDALVMSYMPLVEK
                     IDAQVDHIEDEVFDRPSPNILERIFALKRLLLTMRRILVPQREVLNKLARDDYRVIDP
                     RDRVFFRDIY"
BASE COUNT          718 a          808 c          612 g          743 t
ORIGIN      
        1 agtggcaggg gaaatgccaa cgatattttg cgcgggccat cgttatcctt cgattgagtt
       61 cgatccacgt tcacatacaa ccaacgcgaa gtcttacttt tcgcgccgag tgcgattaac
      121 acaatcccaa gcatcaacgg catgctcatg caatagaacc aaaagttcaa gcccgcattt
      181 tgttgaatac caaacatggc ccaggcactc aaaactgtga agaggatgcc gatccagaga
      241 aatgcgccgg aaaagcgacc cgctctgcgc cggacagttt ccaactccgg cgcctcgctt
      301 cgttccccgc cgaaacctgg tcccacctca atcacctcag gctccgccga tgctgtttcc
      361 gcttctgcat catcatccag cgcgcgcatc aatgtcgccg cttcctctgc gctgatcttg
      421 ccatctgcca ccatttgcag gatcttcctg cgttcttcag aagacattac gaacctcctt
      481 ccagtgccga taaaagtttc tctgcttgtt cagcagtgat ctttttttcc tgaagcatct
      541 ttaagatcgc caagcgttcc tcttccgaag cctgttgttt ggaaggcatg ggaactcctt
      601 tgggtgaaat atcccaattc cagcgaccca cactcaatac agccttcccg ccccggcgcg
      661 atgctttccg ctccgcctca cgagcggttt gttccgcacg acgaatggct tcatctgcct
      721 tgcgctgggc acgggcggca acctggtcta cacggcgtga gatgcgatca ccaaatcccg
      781 accagtccat gccgattccg gcgaaattgc caaattcctc agccgactct cccgcatcgg
      841 actggttcga cacacgaatg tctcctccag cgctcaaggc aattgtggca gagccatctc
      901 ccagagtcac aacccggctg gtagaatcct catcgttttc cacgccatcc caatcaaaat
      961 caatctcgtc agcgctcaag gtcaacatgg catttgccct gggaggcatc accaataaaa
     1021 tatcatcacc cgcgttgaca gcataagcat tgccagattg tggattcaaa taaagcacca
     1081 catcctcacc cacattggcg gtgacattgc cgcgaacatc gcgtaaagca agatcatctg
     1141 tgaccgagtc gagcgaaacg ctgccatcca catcccgaac agatacgtca ccatccgctt
     1201 tacggaccga taagctccct ttcgccccac gcaggctaaa gtccgcgtga accgtaccga
     1261 tcgccacagt gtttgcatca cgaatcgaca aatcaccttc gatctctttt agctcgatcc
     1321 cacccatcac cccccgaatg gaggcatctc cgttaatgct atgaataatg acgtaagcgc
     1381 ctttggggac tcgcaaagaa aggtcatcat tacaggcaat ttttacttca tcaccagact
     1441 ggctgatatt cacatcttct tcatctgtct tgatgaggat gtcatcgccc tcccagccca
     1501 ccaggctcag atcaccattg atggattgga tttcgatttt gggtgtacgt cctgcagaaa
     1561 tggtcctgga cattttttac tcctcgccac gcaacatgcg catggcctga tcggccgtaa
     1621 tcttgcccgc ttccaggtct gcaataacgt tactgcgggt gttatcatct acttcgggtg
     1681 tttcttcacg ccccggttca tatcccaatg cccgaatgac ttcatgcaag cggttgcgaa
     1741 tggttggata cgagaggctc agttcctgtt ccatgcggtt gatcttgcct tcgcagcgta
     1801 cgaaggtgaa tacaaattcc atctgctccg gagaaaggct tgagaattgc cccagagcaa
     1861 agtgaccttc cattgaagta tcacagccat tgcagtgcaa acggacaact tccatttcag
     1921 actggcagat tggacaacga gtaggtgctg gtctcataaa aggcttctcc aagtttataa
     1981 tagcgaggtg atatggttct tgaggtaaaa aaatgaaact tttgtttttt ctaaggaaaa
     2041 atcgaataaa ttaatgtaat acgcctaatt tctaataaaa ttatactcat taaataagat
     2101 ttgtcaatag caacttaaat attatcaact aaaacttcaa tattttcaat tttagtctga
     2161 tattttctaa aaaaacagtt attttggagg ttttttcttg atccgctctc tctattacaa
     2221 acccggccaa tcggttcgca ccaacattcc ccctgctgaa tacccacgcc taattcgcga
     2281 taagaaaggc atgctctggg tggatttcgt ggctgaacct gctgaaagtg cagagcccat
     2341 cctgcgaagt tttggtttcc atcctctcgc catcgatgat gccctacagg aaactcacac
     2401 gccaaaagtg gatgattggg gagactatct ctatatcgtt ctcagcatta tgaattaccg
     2461 cgaggaaagt ggaatgttcg attctgaaat cgatgaactg gacgtcttcc ttggctcaaa
     2521 ttatgtcatc acctttcacg atcaattact gacagctatt gaagatgcct gggtcgcctg
     2581 tcaacgcgac atccgccatg tacaggaggg cgccgaccat ttgctgtatc gtattgtcga
     2641 tgccctggta atgagttaca tgccgctggt tgaaaaaatc gatgcgcagg tggatcatat
     2701 cgaggatgaa gtttttgacc gacctagtcc caacatcctt gaacgaatct tcgccctgaa
     2761 acgcctcctg ctcaccatgc ggcggattct cgttccccaa cgcgaagttt taaacaaact
     2821 tgcccgcgat gattatcgcg tcatcgaccc acgtgaccgg gtcttcttcc gcgatattta
     2881 c
//
LOCUS       DAOQFT010000315         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21124_length_2879_cov_6.614377, whole genome shotgun sequence.
ACCESSION   DAOQFT010000315 DAOQFT010000000
VERSION     DAOQFT010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21124_length_2879_cov_6.614377"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLR93_08575"
     CDS             <1..467
                     /locus_tag="PLR93_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH27060.1"
                     /translation="RFICDADLSMPIEDLGKFLPPQLNDFDIAIGSREAPGAIRYNEP
                     PYRHIGGRAINLAIRFLILPGLRDTQCGFKCFRAETTTRLFEQQTLMGWSFDIELLYL
                     ARRKKLRIREIPIHWYFDAGSKVSAVRDALRMISDIFRIHLNALRGTYDSKA"
     gene            451..1080
                     /locus_tag="PLR93_08580"
     CDS             451..1080
                     /locus_tag="PLR93_08580"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNH27061.1"
                     /translation="MIPKPDLTYENEIWSAYQFIAGMDEAGRGALAGPVSVGAVILPL
                     NHSHLLRALNGVHDSKQLTPRKRSELVPQIQATALAHGVGFASAEEIDQVGIVPATRL
                     AASRALEALSLYPQYLLTDFRLELPELDIPQTAIVKGDQRSLSIACASILAKTARDEF
                     MQNLNEKYPEYRLDHHKGYGTLFHRKKIETLGHSPIHRKTFQLKNNREA"
     gene            complement(1097..1933)
                     /locus_tag="PLR93_08585"
     CDS             complement(1097..1933)
                     /locus_tag="PLR93_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH27062.1"
                     /translation="MNPKRFFVLLTLASLLFFGLWLPAGASSSQQESPPTPTPGTDGR
                     IIYVVQAGDNCFRVAAINGITVEQLRQLNSKLDENCTLTEGQELLIGVAAVATATAPA
                     NVAVGTPTITPTPLSGLTEVCVLLFNDLNGDAMRQDTEPAIAGGAVSVTEANGKYSAS
                     LETVIPSDPTVYQGTCFSNVPEGTYNITMAIPDQYNPTMSLSYSLEVNAGDRAMVDFS
                     AQSKDVVVDQTETEGNAGGASTGLVIFGIVLLLGGAGLGYYAWRSNKPESKLAGGGIL
                     KK"
     gene            1991..>2879
                     /locus_tag="PLR93_08590"
     CDS             1991..>2879
                     /locus_tag="PLR93_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH27063.1"
                     /translation="MKTLIKNGTLITASDTFNADILIEDEKISRIGLNLEADSDHQVD
                     ATGKLIMPGGVDPHVHLDLPMFDTVSSDDHYTGHKAAAFGGTTTAMDFVVLENEGFEH
                     SVDLWMKKAEKAAIDYSFHMNLTRFDGNIAKTIPSLMKMGIQTLKVFTAYNGRLRLDD
                     GGIFKALQIARDNGMLVMAHCENGDVIETLTTQALAEKRTSPIWHALTRPSWGAVESS
                     LRMAAMAEQADAPVYIVHMNAGGEVDMLQYARERGVKVMGETCPQYLFFSVDHLARPD
                     GAKWICSPPMRTEMDNARLW"
BASE COUNT          762 a          761 c          641 g          715 t
ORIGIN      
        1 atcgcttcat ttgtgatgct gatctttcca tgccaattga agatttagga aaattcctcc
       61 caccccagct caatgacttt gatattgcga ttggttcacg tgaagccccc ggtgccatac
      121 gttataacga gccaccctat cgccatattg gcggacgtgc cattaacctt gcaatccgtt
      181 ttctgattct cccgggtctg cgtgacacac aatgcggatt caaatgtttt cgagcggaaa
      241 cgacaactcg attgtttgaa caacaaacct taatgggctg gtcttttgat attgagttac
      301 tctacctggc tcgtcgaaaa aaattacgca ttcgtgaaat ccctattcat tggtattttg
      361 acgcaggcag taaagtaagt gccgttcgcg atgctctacg tatgatcagc gatatcttcc
      421 gtattcacct gaatgccctt cgtggcacgt atgattccaa agcctgattt aacctacgaa
      481 aatgagattt ggtctgcgta tcaattcata gcaggcatgg atgaagccgg acgcggcgca
      541 ctggctggac cggtctcagt tggggcagtg attctccctc tcaatcattc gcatcttctc
      601 cgcgccttga acggagttca cgactctaag caactcacac cccgaaaacg ctccgaactc
      661 gttccacaaa ttcaagcaac tgccctcgcc cacggagtcg gattcgcctc cgctgaagaa
      721 atagatcaag tggggattgt gccagctaca cgtctagctg cgagccgtgc attggaagct
      781 ttatctcttt atccgcagta cttactaacg gatttccgtc ttgagctccc tgagttagat
      841 attccgcaaa ctgccattgt caaaggagat caacgctcat taagtattgc ctgcgcatcc
      901 attctggcga aaactgcccg cgatgagttc atgcaaaatt tgaatgagaa atacccagaa
      961 tatcgcttgg accatcacaa aggctatggg acgctctttc atcgaaagaa gatcgaaacg
     1021 ctcgggcact cccccattca ccgaaaaact tttcagttaa aaaacaaccg cgaggcttaa
     1081 gcctcgcggt cttcatttat tttttcaaga tgccgccacc agctaattta ctttctggct
     1141 tattcgagcg ccaggcataa tacccaagcc cagccccgcc aagtaataaa acaataccga
     1201 agatgacaag cccagtggat gcaccacctg catttccttc tgtctcagtc tggtctacca
     1261 ctacatcttt cgattgagcg ctgaaatcta ccatagcacg atcaccagca ttcacttcca
     1321 gagaatagct caacgacatc gttgggttat attggtcagg gatagccatg gtgatgttat
     1381 atgtcccttc gggaacattt gaaaaacaag taccctgata taccgttggg tctgatggaa
     1441 tgactgtttc taatgaagca gaatattttc cattggcttc ggtcacactt actgcgccac
     1501 cagcaatagc gggttctgta tcttggcgca tagcgtcacc gttaagatca ttgaacaaaa
     1561 gcacgcacac ttctgttaaa ccagaaagcg gagttggagt aatggttggc gtccccactg
     1621 ctacattcgc aggcgctgtt gccgtcgcca cagccgcaac accgatcaat aattcctgtc
     1681 cctcagtcaa cgtacaattt tcatccaact ttgaattcaa ctgacgaagt tgttccaccg
     1741 tgatgccatt gatcgcagcc acgcggaaac aattatcgcc tgcttgaaca acataaataa
     1801 tacgcccatc cgtgccaggg gttggtgtag gaggagattc ttgctggctc gatgacgcgc
     1861 ccgcaggcaa ccacagcccg aagaacagca aactagccag ggtcaacaag acgaaaaaac
     1921 gctttggatt cattgaagaa aattataaca gattcgattt ggtataaaat aaagctgaaa
     1981 tgaggcttgc atgaagactc taattaagaa cgggacctta atcaccgcat ccgacacatt
     2041 caacgcggat attttgatcg aagatgagaa aatctcgcgc atcggactta atctcgaagc
     2101 tgattctgac catcaggtgg atgccacagg taagctgatc atgcccggtg gcgtggaccc
     2161 gcacgttcac cttgacctgc cgatgttcga tactgtttct tccgatgacc attacaccgg
     2221 tcacaaagcc gccgcctttg gtgggacaac cactgccatg gactttgtgg tgctagaaaa
     2281 tgaaggcttt gaacattccg tcgatttatg gatgaagaaa gctgaaaaag cagccatcga
     2341 ctattccttt cacatgaacc tcacccgttt tgacgggaac attgcaaaga caatcccctc
     2401 gctcatgaag atgggtattc aaaccctgaa agtattcacc gcctataacg gacgtctacg
     2461 cctcgacgat ggtggtatct tcaaggcgtt acaaattgcg cgcgataacg gcatgttggt
     2521 tatggcacat tgtgaaaatg gcgacgtcat cgaaaccctc accacacaag cccttgccga
     2581 aaaaagaacc tctcccattt ggcatgccct cacccgccca tcttggggag ccgttgagtc
     2641 cagcttgcgt atggcagcca tggcagaaca agccgatgcc cccgtgtaca tcgttcacat
     2701 gaatgcaggc ggcgaagtgg acatgctcca atacgcccgt gaacgtggtg tgaaagtgat
     2761 gggcgagact tgcccgcaat atctcttctt cagcgttgac catcttgccc gccccgatgg
     2821 cgcaaaatgg atttgttccc cgcccatgcg cacagaaatg gataacgccc gcttgtggg
//
LOCUS       DAOQFT010000316         2876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21166_length_2876_cov_4.638426, whole genome shotgun sequence.
ACCESSION   DAOQFT010000316 DAOQFT010000000
VERSION     DAOQFT010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21166_length_2876_cov_4.638426"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..881
                     /gene="rfbA"
                     /locus_tag="PLR93_08595"
     CDS             9..881
                     /gene="rfbA"
                     /locus_tag="PLR93_08595"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNH27064.1"
                     /translation="MKGIILAGGRGTRLHPLTLAASKQLLPVYDKPMIYYPLSMFMLA
                     GIREILIISTPEDLPSFRRLLGDGKSWGMSFHYAEQAEPRGLADAFIVGESFINGDKA
                     CLILGDNIFFGQGLAAQLQSAAQLKQGALVFAYPVNDPERYGVIEFDAQGKAISLEEK
                     PKQPKSNFAVPGLYFFDERVVAFAKALKPSPRGEIEITDLNRVYLEQGELQVISLGRG
                     VAWLDAGTHESLLQAANFVQAVEDRQGLMISSPEEIAFRKGFIDREQLKVLVKNLGKN
                     SYSEYLDRLANAKV"
     gene            890..1768
                     /gene="rfbD"
                     /locus_tag="PLR93_08600"
     CDS             890..1768
                     /gene="rfbD"
                     /locus_tag="PLR93_08600"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNH27065.1"
                     /translation="MKILLFGKTGQLGWELERTLAALGKVHALGQNELDLQDLTALKK
                     VIHTTKPDVIVNASAYTAVDRAEQEKEIAMRVNAEAPGIMAQAAKELRSVFVHYSTDY
                     VFDGHKTTPYEENDKVNPLNVYGQSKLNGEQAVAEARGAHVILRTSWVYSLRGDGFVS
                     KVLRWARHQETLKIVSDQVGSPTWARALAETTALMLAGSAPNPFDYFSERSGVYHLGG
                     LGAVSRLDFAQEILRLDPHKKEQICQRLEPALTADFPTTALRPLTTPLNCSHFSKTFG
                     VSLPTWQEALHLAMDV"
     gene            1982..>2876
                     /locus_tag="PLR93_08605"
     CDS             1982..>2876
                     /locus_tag="PLR93_08605"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNH27066.1"
                     /translation="MKFKNNTPTVITGLVQVFVVGTFILSLFVTTPVGAVSGTDLVVT
                     NVVIAPTNPLPGEAISVNVTVLNQGDLTSNSIVYRHVFVDTVNPNSLPTDPGTGCPST
                     DDPYENYFRSDLNDGIPAGGSDTKAVSIPNGLTAGTHQLWIYVDATCINDESNETNNV
                     YGPISLTLGIPINVTVGNTVNTLHYLPSAGSSLRKSYIGIDDGPVKVDGNSDNIIAAE
                     RVIYNPGGIPVSFTELMGLPSTLVDDEYWFPWYNNVNVDTQLRFGNVSGSPASVRVII
                     GGVEMPGSPFALTASGAGQSLR"
BASE COUNT          734 a          603 c          726 g          813 t
ORIGIN      
        1 agaaaaacat gaagggtatt attttggcag gcggacgcgg tacaaggttg cacccgctca
       61 ctttggcagc gagcaaacaa ttgctacccg tatacgataa acccatgatc tactatccgc
      121 tttctatgtt tatgctggca gggatacgtg agattctaat cattagcaca ccagaagacc
      181 tgccttcgtt ccggcggtta cttggcgacg gcaaatcttg gggaatgtct tttcattatg
      241 cagaacaagc cgagccgcgt ggtttggcag atgcttttat tgtgggggag agttttatca
      301 atggagataa agcttgcctg attcttggcg ataatatctt ctttgggcaa gggttggcag
      361 cgcaattgca atctgctgca caacttaagc agggtgctct ggtctttgct tacccggtca
      421 atgaccctga acgctatggg gtgatcgaat ttgatgcgca aggcaaagcc attagcctgg
      481 aagaaaaacc aaaacagcct aaatctaatt ttgcagtacc gggcttatat tttttcgatg
      541 agcgtgtcgt tgcttttgcc aaagcgctta agccttcacc gcgtggtgag atcgagatca
      601 ctgaccttaa ccgtgtgtat cttgaacagg gtgagttgca ggtcatttcg cttgggcgcg
      661 gcgtcgcctg gctggatgct ggcacacatg agtcgctgtt gcaggcggca aattttgtgc
      721 aagcggtgga agaccgccaa ggtttgatga tctcttcgcc agaggagatc gcttttcgca
      781 aaggctttat tgaccgcgag caactaaaag tgttggtgaa aaatttaggc aagaatagtt
      841 atagcgaata tcttgatcgc ctggcaaatg ccaaggtata aagcttttta tgaaaatctt
      901 attatttgga aagaccggtc agcttggctg ggaattggaa cggacccttg ccgctttggg
      961 gaaggtgcat gcgcttgggc aaaacgaatt ggatttgcaa gacctgacag ccttgaaaaa
     1021 agttattcac actaccaagc ccgatgtgat tgtgaatgct tcggcatata cggcggtaga
     1081 ccgtgccgag caggaaaaag aaattgccat gcgcgtgaat gccgaggctc ccggcattat
     1141 ggcacaggcg gcaaaagaac ttcgttctgt ctttgtccat tattcaacag attatgtttt
     1201 tgatgggcat aagaccacgc cttatgaaga aaatgataag gtgaacccgc tcaacgtgta
     1261 tggtcaaagc aaattaaacg gcgaacaagc cgtggcggaa gcgcgaggcg cgcacgtcat
     1321 cctgcgtacg agttgggtct atagtttaag gggcgatggc ttcgtctcaa aggttttgcg
     1381 ttgggcgcgt catcaagaaa cgttgaagat cgttagcgat caagtgggta gccccacctg
     1441 ggcgcgggcg ttggcagaaa caacggctct gatgttggca gggagtgccc ccaacccttt
     1501 tgattatttt tctgaacgtt cgggcgttta tcaccttggc gggctgggag cggtctctcg
     1561 tttggatttt gcgcaagaga tattgcgact cgatccccat aaaaaagagc agatttgcca
     1621 acgcctcgaa cccgcgctga cggcggattt cccaaccaca gccttgcgcc cgctgacaac
     1681 gcctttgaat tgctcgcact tcagcaagac ctttggggtg agcctgccta cctggcagga
     1741 ggcattacac cttgccatgg atgtatagct tatgggacgg gacgatggat tatccttaca
     1801 gaatagaaaa tccctctcta gggaatatgt tttataatta ttatgtactt gcctgatact
     1861 ccatatgact tttgcaatgt agtctgcgct ctttactact ataaaacacc tacttcagga
     1921 cttcaggtat ttattttatg ctgtttttgt accaactgat ttgaagaaag gtttaaagat
     1981 tatgaaattc aagaataata cccccaccgt tattacggga ttggttcaag tctttgttgt
     2041 cggaactttt atcctttcat tattcgtaac tactcctgta ggtgctgtaa gcggcactga
     2101 tttggttgtt actaatgtcg ttattgctcc tactaaccct ctccctggag aggctatttc
     2161 ggttaatgtt actgttctta atcaaggcga tttgacaagt aatagtattg tgtatcggca
     2221 tgtttttgta gatactgtta accctaatag tttacccaca gatccgggaa caggctgccc
     2281 gtcaactgat gatccatatg aaaattattt tcgttctgat cttaacgatg ggattcctgc
     2341 gggaggatct gatacaaagg cagttagcat tccaaatgga ctgactgcag gaacacacca
     2401 actatggatt tacgttgatg ctacatgtat taatgatgaa agcaatgaaa ctaataatgt
     2461 ttatggacca atatcattaa ccttggggat tcctattaat gtgactgttg gaaacactgt
     2521 taatacctta cattatttgc catccgctgg aagcagttta cgaaaaagct atattggtat
     2581 tgatgatgga ccagtgaaag tggatgggaa tagtgataac attattgctg ctgagcgtgt
     2641 catctataat cctggcggta ttcccgtaag ctttactgaa ctaatggggt tgccaagtac
     2701 acttgttgat gatgagtact ggtttccctg gtacaacaat gttaatgttg atacacagtt
     2761 gcgctttggg aatgtcagcg gatcacctgc ctcggtgcga gttattattg gaggagtaga
     2821 gatgcccggc agtccctttg ccttgacggc ttcaggcgca ggtcaaagcc tgcgag
//
LOCUS       DAOQFT010000317         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21187_length_2874_cov_8.394111, whole genome shotgun sequence.
ACCESSION   DAOQFT010000317 DAOQFT010000000
VERSION     DAOQFT010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21187_length_2874_cov_8.394111"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLR93_08610"
     CDS             <1..431
                     /locus_tag="PLR93_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH27067.1"
                     /translation="EANDGSFTGNFDSLDDGRSFAESFVQEGADIIMPVAGPVGLGSA
                     AYCQESGACKIIGVDVDWTVSAPEYKDIILTSVLKNINVAVYDAIQAEVDGTFAGGVY
                     VGTVENGGVGISDVAGASDDLKAQLDAVQQGIIDGSIATR"
     gene            622..>1172
                     /locus_tag="PLR93_08615"
     CDS             622..>1172
                     /locus_tag="PLR93_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH27068.1"
                     /translation="MTNVLELRGITKRFPGVLANDKISITLREGKILALLGENGAGKS
                     TLMNILYGLYKPDEGQIIVRGKEVDIQGPNDAIAQGIGMVHQHFMLVPVMTVTENVML
                     GIEPTKNGIFLDKEKVANRIREISDEYGLEVDPNAYIKDLPVGIQQRVEIIKVLYRHA
                     DILILDEPTAVLTPQEVEGLFKI"
     assembly_gap    1173..1272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1273..1770
                     /locus_tag="PLR93_08620"
     CDS             <1273..1770
                     /locus_tag="PLR93_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HNH27069.1"
                     /translation="GLILSFPIHDNMMLCTYYKDPFAKGISLQQKTIFENAEQLVKQY
                     DVRTPNIYVNAGTLSGGNQQKVIVAREFSRPIELLIASQPTRGLDVGSIEYIHSQIIK
                     KRDEGSGVLLVSSELDEILALSDRIAVMYKGQIMAIVDASKATKEQLGLLMAGVHPSE
                     TSNKN"
     gene            1782..>2874
                     /locus_tag="PLR93_08625"
     CDS             1782..>2874
                     /locus_tag="PLR93_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27070.1"
                     /translation="MTTTATPAPDAGTKETKRNSLWQELLIPILAVVTGLIVGAIVIF
                     VSGDNPFVAYGALFAGSFGTPADYVEGFKVLFSTGDTAALIKAIYPFTESLVTATPYI
                     FAGLSVALGFRCGLFNIGAEGQFFIGALCSAFVGYSLKGLPMIVHLPLAILGGALGGA
                     IWGAIPGYLKARFGAHEVVNTIMMNWIAFRLSDWLLSGPMKSSGFRPVTPNVEASAEL
                     PRFFPEPLRFNLGFFLALLAAYLLYWFLFKTTLGFEIRSVGANPDAAKYAGMNIIRNF
                     VLVMFLAGGLAGLAGTAQVLGVDHWVGQGFSAGYGFDAIALALLGRSHPAGVVLAALL
                     FGFLRSGATDMQSMAGIPIDIISIIQGMVI"
BASE COUNT          621 a          694 c          695 g          764 t
ORIGIN      
        1 ctgaagccaa cgatggttcc ttcaccggta acttcgactc cctcgatgat ggtcgctcct
       61 tcgccgaatc cttcgttcag gaaggcgctg acatcatcat gcctgtggct ggacctgttg
      121 gtctcggctc tgctgcttac tgccaggaat ccggcgcctg caagatcatc ggcgtggatg
      181 ttgactggac cgtttctgct cctgaataca aggacatcat cctcaccagc gttctcaaga
      241 acatcaacgt tgctgtgtac gatgccattc aggctgaagt tgacggcacc tttgctggtg
      301 gcgtgtatgt tggtaccgtt gaaaacggcg gcgtcggtat ctccgatgtt gctggtgctt
      361 ctgacgacct caaggctcaa cttgacgctg ttcaacaggg catcatcgac ggctccatcg
      421 caaccagata atctggctct actgcactac aaaagccgga ggaggggttt ccctcccccg
      481 gctttttatt tttcttattg ctcgtagatc tcaaaggttt tttcaatttg tcatacattt
      541 tccatgcacc atcctttata attggggcgt gcaaaaaaaa ccttttggat ctatcaaagt
      601 aaagatagtg gagggtatgg catgaccaat gttttggaac tgcgtggcat taccaaacgt
      661 ttccccggtg ttcttgcaaa cgacaaaatc agtattactc tgcgtgaggg caaaattctc
      721 gctttgttag gtgagaacgg tgcaggcaaa agtacattga tgaatatcct ttatgggttg
      781 tacaaacctg atgaaggaca gatcattgtc cgcggtaaag aggtggatat tcaaggaccc
      841 aacgacgcca tcgcacaagg gatcggcatg gtgcatcagc actttatgct cgtccccgtg
      901 atgacggtca ccgagaatgt aatgctcggt attgaaccca caaagaacgg tatattttta
      961 gacaaagaaa aggtcgcgaa ccgcatccgt gaaatttcgg atgaatacgg tttggaagtt
     1021 gacccgaatg cctatatcaa agatctgcct gtgggcattc agcagcgtgt ggagatcatt
     1081 aaagttctct atcgccatgc agatattctc atcctagacg agccgacagc ggtactcacc
     1141 ccgcaagaag tggaaggctt gttcaagatc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnggcttgat cctttccttc cccatccacg acaatatgat gttatgcacc
     1321 tactacaagg acccctttgc aaaaggcatt tccttgcagc agaaaaccat ctttgaaaac
     1381 gccgagcaat tggtaaaaca atatgatgtg cgcaccccga acatctatgt caatgcaggg
     1441 acgctttccg gtggtaatca gcaaaaagtg atcgtggcgc gtgaattttc acgtcccatt
     1501 gaattattga tcgcctcaca accgacccgt gggttggacg ttggctcgat tgaatacatc
     1561 cactcacaga ttatcaaaaa gcgcgacgaa ggttctggcg ttctgctcgt ttcatccgaa
     1621 ttggatgaaa ttctcgctct ctcagaccgc attgctgtga tgtacaaagg tcagatcatg
     1681 gcaatcgttg atgcaagcaa agcaaccaag gaacaacttg gtctgctcat ggcgggtgtt
     1741 catccctccg aaacctcgaa caagaattag gagttgtatc tgtgactact acagctactc
     1801 ccgccccaga tgctgggaca aaagaaacga aacgtaattc gctttggcag gaattgttaa
     1861 tccctattct tgctgttgtg accgggttga tcgttggcgc cattgttatc ttcgtcagcg
     1921 gcgacaatcc ctttgttgca tacggtgctc tctttgccgg gtctttcggt acccctgccg
     1981 attatgttga aggcttcaag gttttattct ccacaggaga tacagccgca ttaatcaaag
     2041 ccatttaccc atttactgaa agtcttgtaa cggctacgcc ctatattttt gcagggcttt
     2101 cggtggcact cggctttcgt tgtggtttgt tcaatatcgg tgctgaaggt cagttcttca
     2161 ttggtgctct ttgctctgcc tttgttggct acagcctcaa gggacttccc atgatcgtcc
     2221 atttgccgct tgccatttta ggcggcgcct tgggcggagc gatctgggga gccattccgg
     2281 gctatctcaa ggctcgcttt ggcgcccatg aagtggtcaa caccatcatg atgaactgga
     2341 tcgctttccg attgagcgac tggctcttaa gcggaccgat gaagtcttct ggttttcgcc
     2401 cggtgacccc caatgtggaa gcctctgcag aattgccacg cttctttccc gaaccgttgc
     2461 ggttcaacct cggattcttc ctcgccttgc tggcagcgta tctcctctac tggttcttgt
     2521 tcaagaccac gctagggttt gagatccgct cggtgggcgc aaacccagat gccgcgaaat
     2581 atgcaggcat gaacatcatc cgtaactttg tattggtcat gttcctagcg ggtggcttgg
     2641 cgggcttggc tggaactgct caagttctcg gcgtggatca ctgggtgggt cagggctttt
     2701 cagccgggta cgggtttgat gcgattgcgt tggctttgct gggtcgcagt catccggcag
     2761 gagtcgtcct tgcagcgctg ctgtttggct tcctacgtag tggtgccacc gatatgcagt
     2821 ctatggctgg catccccatc gacattatct cgatcattca aggtatggtg attg
//
LOCUS       DAOQFT010000318         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21188_length_2874_cov_5.502661, whole genome shotgun sequence.
ACCESSION   DAOQFT010000318 DAOQFT010000000
VERSION     DAOQFT010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21188_length_2874_cov_5.502661"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLR93_08630"
     CDS             <1..477
                     /locus_tag="PLR93_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH27071.1"
                     /translation="HGGWTLPYWHDEIGAHFGQIFADADTLLLGRKTWQTHGEAFEPN
                     PAEDPFNGFKKFVVSSTLKSADAWRDSTLIRGNVIEEVRKLKSQPGKNIIMDGSSVLV
                     QALIEHDLVDELSLHVYPLVLGGGKRMFPAGKRVSLNLIESSALPTGVVYQRFQLT"
     gene            512..1384
                     /locus_tag="PLR93_08635"
     CDS             512..1384
                     /locus_tag="PLR93_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNH27072.1"
                     /translation="MKIHLVDGTYELFRAHFGAPPKKSKSGQEVGATLGMIRSMLLLL
                     SDPEVSHVAVAFDHVIESFRNKMYASYKSSEGVDPVILNQFPVAEAAIKALGLVTWPM
                     VKYEADDAIATAVAKFKKVKSVEQIVICSVDKDLTQMVDGDRVICWDRRREILLNEKG
                     VVEKFGVSPESIPDFLALVGDSADGYPGIQGWGEKSTSAVLAKYKHIEAIPKDPNKIP
                     LGLGRATTLLENLQSNYKDALLFRELSTLRTDVPLKETLADLKWQGVYPRFKKVCEDL
                     GNQSIPERISKWRK"
     gene            1399..1971
                     /locus_tag="PLR93_08640"
     CDS             1399..1971
                     /locus_tag="PLR93_08640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH27073.1"
                     /translation="MLNLIITSLLFLIVILSALWMIVPAFYGPPSVPTRMNRIRKALQ
                     LASLQPGETVYDLGAGDGRVLLMAASEFHANAVGVEVGPVQCLLIKLRALSSGQGDKI
                     SVKWGSYFKVDLKDADVAFIYATSKETIKLAAHLKNQMKPGSRLVSISADFPEWEPTT
                     IDELELIFLYNMPPVAGNPTTYLMKKSNSS"
     gene            1976..>2874
                     /locus_tag="PLR93_08645"
     CDS             1976..>2874
                     /locus_tag="PLR93_08645"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNH27074.1"
                     /translation="MLNIDIETARRFILGKQGLWPGRRWRGLKGTDHAMRTMEYLQLD
                     PLQIIARSQDISLYSRVLNYKPEMWEDAAYKQRNFFDWGGWLAARPMEELPYWRVVMH
                     RERDGGYGDSRLRQMGKEHAKAILEVRAILKDQGTVGNRDFEAADRKKTQSYRGRKDS
                     ALALYYLWRIGDVMTHHRERFERVYALTENVAPKELIYEKTQEEVDRFLVRKEISFGG
                     ITRIQRTGDSYGRGEPDRAAKKLLENMLADGEIIEVKVDGWKTIQYALAEDEKVISGL
                     SAGRIPKAWKPVDTTTTEEAVFLA"
BASE COUNT          747 a          589 c          790 g          748 t
ORIGIN      
        1 cacggcggct ggacgcttcc atactggcat gacgagattg gtgcgcactt cggtcagatc
       61 tttgcagacg ccgatacttt gttgttaggg cgcaagacct ggcagacgca tggagaagcc
      121 tttgagccga atccggcaga agatccgttc aatggcttca agaagtttgt cgtttcatcc
      181 acattgaagt ctgccgatgc ctggcgtgat tcgactctca ttcgtggaaa tgtgatcgag
      241 gaagttcgca agttgaaaag ccagcccggc aagaacatca tcatggatgg aagcagtgtt
      301 ctggttcagg ctttgatcga acatgatttg gtagatgaac tttcactgca tgtgtatccg
      361 ctggtattgg gtggcggtaa acggatgttt ccggctggga aacgtgtcag tcttaatctg
      421 atagagtctt ctgcgcttcc aactggcgtt gtctatcaac gttttcaact aacctaaaaa
      481 aacaaagagc aagagcatac aaggagtctg catgaaaatt catttggtag atggaaccta
      541 cgaattattc cgtgctcatt ttggcgcacc gcccaagaaa tctaaaagcg gacaggaagt
      601 tggcgcgact cttggaatga tccgcagtat gctcttgctg ctttctgacc cagaagtgtc
      661 tcacgtcgct gtggcgttcg accatgtgat cgagtctttt cgcaataaga tgtatgctag
      721 ctataaatcc agtgaaggtg tggatccggt cattttgaat cagtttccgg tggctgaggc
      781 ggcgatcaaa gccttgggat tggtgacctg gcctatggtc aagtatgaag cggacgatgc
      841 gattgctaca gcggtagcga agttcaagaa agttaagtcg gttgagcaga ttgtcatctg
      901 ttctgtcgat aaagacttga cccaaatggt ggatggtgac cgtgttattt gctgggatag
      961 acgccgtgag attttgttaa atgaaaaagg tgtggtcgag aagttcggtg tcagtcctga
     1021 atcgattcct gacttccttg ccttggtggg ggattctgca gatgggtatc ctggtattca
     1081 aggctggggt gaaaagtcca cttcggcggt gttggcgaag tacaagcaca tcgaagcgat
     1141 tcccaaagac ccaaataaaa ttccgcttgg tttggggcgt gccacaactt tacttgaaaa
     1201 tttgcagagt aactacaaag acgcactatt gtttagagag ttatccactt tgcgcacgga
     1261 tgtaccgttg aaagagacgc tggcagattt gaaatggcag ggtgtttatc cgcgctttaa
     1321 gaaggtgtgt gaagacttgg gaaatcaaag cattcctgaa cgaatcagca agtggagaaa
     1381 gtagattcgt tcttgtttat gcttaactta attatcacct cgcttctgtt tttaattgtc
     1441 atccttagcg cattgtggat gatcgttccg gcattttatg gtccgccttc cgtgcccacg
     1501 cggatgaacc gaatccgcaa agcgttgcaa cttgccagtc tccaacctgg agagactgtc
     1561 tacgatcttg gcgctggcga tggacgtgtt ttgttgatgg cagccagtga atttcatgcg
     1621 aatgctgtgg gtgtggaagt gggacccgtt caatgtttgc tgattaagtt gcgtgcactt
     1681 agcagcgggc agggcgataa gattagtgtg aagtggggga gttatttcaa ggttgacttg
     1741 aaagatgctg atgttgcttt tatttatgcc acttcaaaag agacgataaa actcgcggct
     1801 cacttgaaaa atcaaatgaa acccggctca aggcttgtct ccatttctgc agactttccc
     1861 gaatgggaac caactacgat cgatgagctg gaattgattt tcttatataa catgccgcct
     1921 gtagcgggaa acccaactac atatttaatg aagaaatcga attcctccta aacttatgtt
     1981 gaatatagat atagaaactg cccgccgttt tattcttgga aaacaaggct tgtggcccgg
     2041 tcgtcgctgg cgcggactta aaggcactga ccatgcgatg cgtaccatgg aatatttaca
     2101 gcttgacccg ttgcagatca ttgcacgcag tcaggatatt tccttatata gccgtgtcct
     2161 caactacaag ccggaaatgt gggaagatgc cgcctataaa cagagaaatt tcttcgattg
     2221 gggcggctgg ctggcggcgc gcccaatgga agagttgccc tattggcggg tagtgatgca
     2281 ccgtgaacga gatgggggct acggtgactc acgtctgcgc cagatgggaa aggaacatgc
     2341 caaggcaatc cttgaagtcc gcgctatttt gaaagatcaa gggacggtag gcaatcgcga
     2401 ctttgaggca gcagaccgca agaagactca aagttatcgt gggcgaaaag atagcgcgtt
     2461 agcattgtat tatttgtggc gcatcggtga tgtaatgacg catcatcgcg aacgatttga
     2521 gcgggtctat gcactgacgg agaatgttgc cccgaaagaa ttgatctacg aaaagactca
     2581 agaagaagtg gatcgttttc tggttaggaa agagatcagc tttggcggca tcacacgcat
     2641 tcaacgcacc ggcgattcgt atggacgcgg cgagccagat cgggcagcga agaaactgct
     2701 tgaaaacatg ttggctgatg gcgagattat cgaagtaaaa gtggatggat ggaagacaat
     2761 ccaatatgcg ttggcggaag atgagaaggt gatttcaggc ttaagcgcgg ggcgtattcc
     2821 aaaagcatgg aaacctgtag acacaacaac gactgaagaa gctgtttttc ttgc
//
LOCUS       DAOQFT010000319         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21347_length_2860_cov_7.090909, whole genome shotgun sequence.
ACCESSION   DAOQFT010000319 DAOQFT010000000
VERSION     DAOQFT010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21347_length_2860_cov_7.090909"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLR93_08650"
     CDS             complement(<1..373)
                     /locus_tag="PLR93_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH27075.1"
                     /translation="MLKKKIAFIGPGVMAEAMIAGLLNKKLADAKNIIASGPRDARGE
                     ELTKKYGIKFTTDNSAAIHEADVVVLSVKPQRLTEVMKGLKGVRPDALVLSIIAGATI
                     KKIGAGLKHKAIVRSMPNTPGQ"
     gene            complement(451..1782)
                     /locus_tag="PLR93_08655"
     CDS             complement(451..1782)
                     /locus_tag="PLR93_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNH27076.1"
                     /translation="MKKSFNRLWFIPIFIVLSIGVYYLPPVHSRLAWRIELVRTQIKY
                     WLNPPDEAVFQPSGETSPLTVETAVATTRAEFLLTLTPTSTPTPELVATIEPTITSTP
                     LPTTVMLDGVKYEHQHGRTNYCGPANFSMALTYWGWDGNRDVIGKVVMPVNEKDKNVM
                     PYELQDFITNNVPGMTSIIRNGGDFETLKRLISAGYPVITEKGIYETDLNGKFSWMGH
                     YAFVTGYDDANQVIIYQDSYQPDPETNPGPNRRMGYEAFVEGWRAFNYVFVVVFPVEK
                     QEDVLALLGPLADETQANRHALEVAKAESQTLYGIDQYFAWFNIGTSHVDLLEYADAA
                     SAYDYAFSLYADLNVDDTARPYRMMWYQTGPYKAYFYSNRLTDVITLADTTLNDTIAE
                     PVLEESLYWRGRAYVMSGDTNRAVKDFMAALKVHPKWEPAVQALQDLGLQP"
     gene            complement(1846..>2860)
                     /locus_tag="PLR93_08660"
     CDS             complement(1846..>2860)
                     /locus_tag="PLR93_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNH27077.1"
                     /translation="HLQLKLSRSKFEQMTEDLLQRCRKPFEAALKDAGMDASKLDEVV
                     LVGGSSRMPMVQDLVRKLTNGKDPNKGVNPDEVVAIGAAIQGGVLGGDVKDILLLDVT
                     PLSLGVETMGNVMTVMIERNTTIPVRKTEVYSTAADNQTAVDIHVLQGERPMAGDNMS
                     LGRFRLDGLPPAPRGVPQVEVTFDIDANGILHVTAKDKATGKEQKVTITASTNLNKSD
                     IERMVQESKQNEAADKKRREAIQVKNDADNMTYQIEKALRDLGDKVPSAEKASIETKV
                     NELKQAAQGDDIEHIKKLTEELQNTFHALSQQMYAQGQGGQPQPEGGPAPDGNVVDGE
                     VRE"
BASE COUNT          643 a          697 c          743 g          777 t
ORIGIN      
        1 tctgtcccgg ggtattgggc atcgagcgga cgatggcttt gtgtttcagc cctgccccaa
       61 tctttttgat ggtcgcacct gcaataatgg ataagaccaa cgcatctggt cggactccct
      121 tgagtccttt catcacttcg gtcaggcgct gcggtttgac ggagagcacg accacgtccg
      181 cttcgtgaat ggcggcagaa ttatcggtgg tgaatttgat gccatatttc ttggtcagtt
      241 cttcgccacg tgcatctcgc ggaccagaag cgataatatt tttggcatct gccaattttt
      301 tattgagcag tccggcgatc atggcttctg ccatcacacc aggaccgata aatgcaattt
      361 tcttcttaag catgagtctc tcctattacg ttacgtgaaa gatattatac aaagaaaaaa
      421 ggatgcaaaa gcttctgcat cccttcggtt ttacggttgc aagcccaaat cttggagcgc
      481 ttgcacagcg ggctcccatt tggggtgaac tttgagtgct gccataaaat ctttcacggc
      541 tcggttggta tctccagaca tcacatacgc ccgtccgcgc cagtataagg actcttccaa
      601 cacgggttcg gcgatggtgt cgttcagggt cgtatctgcc agagttatga catctgtgag
      661 gcggttcgag tagaaatagg ctttatacgg accggtctga taccacatca tgcgataggg
      721 acgggcggta tcgtcaacat tcaaatccgc atataagcta aatgcataat cataggcgct
      781 ggctgcatcg gcgtattcga gcaagtctac atggctggtg ccgatgttga accaggcaaa
      841 atattggtca atgccataaa gggtttggga ttcagctttg gcgacctcaa gcgcatggcg
      901 attggcttga gtttcatctg ctaacggacc gagcaatgcc agtacatcct cttgtttttc
      961 tacagggaag accaccacaa aaacataatt gaaggcacgc caaccttcta caaaggcttc
     1021 gtatcccatt ctgcggtttg gaccggggtt tgtttctggg tcgggttgat aactatcttg
     1081 atagatgatg acttgattag catcatcgta accggtgaca aaggcataat gtcccatcca
     1141 actgaatttg ccgttgaggt cggtttcata gatgcctttt tcggtaatga ccgggtagcc
     1201 tgccgagata aggcgtttaa gcgtttcaaa gtcaccgccg ttgcgaatga tagacgtcat
     1261 accggggaca ttgttcgtga tgaaatcctg caactcgtat ggcatcacgt tcttatcttt
     1321 ttcattgaca ggcattacaa ctttgccgat cacatcacgg ttgccatccc aaccccaata
     1381 ggtaagcgcc atagagaagt ttgcaggtcc gcagtagttt gtccgcccgt gttggtgttc
     1441 atacttcacg ccatcgagca tgacagtagt gggtaaaggt gtggatgtaa tggtcggctc
     1501 aatggtggca accagttctg gcgtcggggt ggaggtgggc gtcaacgtca ataaaaattc
     1561 agcacgcgtt gtggcaaccg ctgtctcgac ggtcaatggg ctggtttctc cgctgggttg
     1621 aaagacagct tcatcgggcg ggttgagcca atatttgatt tgcgtgcgca cgagttcaat
     1681 acgccatgca aggcggctgt gaacaggcgg aagataataa acgccaatac tgaggacaat
     1741 gaaaatggga ataaaccaaa gacggttaaa actttttttc atgtaaagat tatacataca
     1801 aaaagaactc cgaagtttct gaggcttcgg agttctgatt attttttact ctcgtacttc
     1861 accgtcgacg acgttgccat ccggcgcggg accgccctcg ggctggggtt gcccaccctg
     1921 accttgagcg tacatctgct ggctgagtgc atggaaggtg ttctgcaatt cttcggtcag
     1981 cttcttgatg tgctcgatgt catcgccttg tgcggcttgc ttcaactcat tgaccttggt
     2041 ttcaatggaa gccttttccg ccgatgggac tttatcgcca agatcacgca atgccttctc
     2101 aatctgatac gtcatattat ccgcatcgtt cttgacctgg atcgcttcac ggcgtttctt
     2161 atcggcagct tcgttctgct tggactcttg caccatgcgc tcgatgtcgc tcttgttgag
     2221 attggtcgaa gcggtgatgg tgactttctg ttccttgcct gtggctttgt ctttggcggt
     2281 cacatgcaag ataccgtttg cgtcgatatc gaaggtcact tcgacctgcg gaacgccgcg
     2341 tggtgctggt ggaagtccat cgaggcggaa gcgtcccagg ctcatgttat cgccagccat
     2401 tgggcgttcg ccttgcaaca catgaatgtc tacggcggtc tgattatctg cagcggtgga
     2461 gtaaacttcg gtcttgcgaa caggaatggt ggtattgcgt tcgatcatga cggtcatcac
     2521 gttgcccatg gtttccacgc caagagagag cggggtcaca tcgagcaaga gaatgtcttt
     2581 gacatcgcca ccaagcacgc caccttgaat agctgcacca atggcaacca cttcatcggg
     2641 gttcacgcct ttgttcgggt ctttgccgtt ggtgagcttg cgcaccagat cctgaaccat
     2701 tggcatacga gaagaaccac caactaagac aacttcgtcg agcttgctgg catccatgcc
     2761 ggcatctttg agcgccgctt caaacggttt acggcagcgc tgcaacaggt cttcggtcat
     2821 ttgctcaaat ttagagcggc tgagtttcaa ctgcaagtgt
//
LOCUS       DAOQFT010000320         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21373_length_2858_cov_5.755262, whole genome shotgun sequence.
ACCESSION   DAOQFT010000320 DAOQFT010000000
VERSION     DAOQFT010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21373_length_2858_cov_5.755262"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="der"
                     /locus_tag="PLR93_08665"
     CDS             complement(<1..1206)
                     /gene="der"
                     /locus_tag="PLR93_08665"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNH27078.1"
                     /translation="MKPIVALVGRPNVGKSTLFNRLVGERMAIVDDTPGTTRDRLFGD
                     AEWNARPFTVIDTGGIDPTHGGRTPLSVGSADFIGDIRRQAQAAIQEADAILFVVDGE
                     TGVTEPDREVADILRRMQKKQPDGSFLPPIILVVNKCESRERRDSVGEFYELGLGEPF
                     AISAIHGTNTGDLLDALVSSFPTQNEDEEDNNIKIALVGKPNAGKSSLLNKLVGEERV
                     IVSPIAGTTRDAIDMELEFGGLPITLIDTAGIRRRGKIEKGVEEYSVIRSFKAIERAD
                     VALLMIDATTGITSQDAHIAGFIKDEWKSCVVLVNKWDAIEKDGTTMDAYTEKILNDL
                     NFVSYVPILYISAKTGQRVDQVLPLALRVQEERLARLTTSKINEIIHAAQDAGLGWQK
                     TRRRMAEMQS"
     gene            complement(1222..2475)
                     /locus_tag="PLR93_08670"
     CDS             complement(1222..2475)
                     /locus_tag="PLR93_08670"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNH27079.1"
                     /translation="MVRWILENYRTFLWAFALAVAVWISSVTSADPDETRALDNPISV
                     EVIGQSSNLVFSEPIAKEVSVTLRAPSSVWALIDDDPSIVRAILDVSGLSAGEHTVDL
                     QIQVNARPVQIVSVSPKTFVFSLEPLITKSMDVDLRVAGEVAVGYQVGDSTLDPVSVV
                     VSGAQSQVEKVSRARVSIDLNRIRENFDQAVKVEVLDDRGQVVNGVTVSPESVHIVLP
                     VSQQGGYRDVAVKVTTLGRVASGYRLTDISVSPPVVTIYSTDPDLVNSLPGVLETQPL
                     DLQNAQDDIATRLKLSLPSGISVIGEQTVFVQAGVSPIESSVTISSENIEILGLDESL
                     NVQISPSNVDIILSGPLPLLETLTRQSVRVTVDLTGLTEGKYQLSPNVEVLVANVAVE
                     SILPNTIEVTLTPLNSSTPTPTPKP"
     gene            complement(2479..>2858)
                     /locus_tag="PLR93_08675"
     CDS             complement(2479..>2858)
                     /locus_tag="PLR93_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HNH27080.1"
                     /translation="FLQIFYPNTPLHDGGVIVVDSKVVAASCVLPLSASGILNRTPER
                     QMGLRHRASLGISEVSDAIAIVVSEETGAISIAHSGRMLRRLDPERLENILTAFFRPS
                     GAEQRIGFIGKLFSGKKSRKEEK"
BASE COUNT          722 a          733 c          719 g          684 t
ORIGIN      
        1 actctgcatc tctgccattc ttctacgagt cttctgccat cctaggccgg catcttgcgc
       61 ggcgtgaatg atttcattga tcttcgaagt cgtcaggcgg gcgaggcgtt cttcttggac
      121 acgcaatgcc aagggtaaga cttggtccac gcgctgaccg gtcttggcag agatataaag
      181 aatgggaacg tacgaaacaa aattcaggtc gttcagaatt ttctctgtgt aggcatccat
      241 cgtggtgcca tctttttcaa tggcatccca tttgttgacc aaaaccacac aagatttcca
      301 ctcgtctttg ataaaaccag caatgtgggc atcttgtgag gtgataccgg tggtggcatc
      361 gatcatcagt aacgcaacgt ctgcacgttc aatggctttg aaagaacgga taacgctgta
      421 ctcttccacg cccttttcaa tcttgccacg gcgacggatg cccgcggtat caatgagagt
      481 gatgggcaac ccgccaaatt ctaattccat gtcgatggca tcacgggtgg tccctgcaat
      541 tgggctgacg atcacacgtt cttcgccaac gagtttattg agcaggcttg atttcccggc
      601 attgggtttg cccaccaagg cgatcttgat attgttgtct tcttcatctt cattttgagt
      661 cgggaacgaa gagacaagtg catcgagcaa gtcaccagtg ttggtgccgt gaatagcaga
      721 aatggcaaag ggttccccta aacctagctc ataaaattca cctacagaat cacggcgctc
      781 tcttgattca catttgttta cgactaatat gatcggaggc agaaaagaac catcgggctg
      841 tttcttttgc atgcggcgca aaatatctgc cacttcgcgg tctggctcag tgacgccggt
      901 ttcgccatcg acgacaaaga ggatggcgtc ggcttcttga atggcagctt gggcttgtcg
      961 gcggatgtct ccgataaaat cggcagaacc aactgagagc ggagtccgac cgccgtgagt
     1021 cgggtctata ccgcctgtat caataacagt gaagggacgg gcgttccact ctgcatcacc
     1081 aaaaaggcgg tcacgggtgg tgccgggggt atcgtcaacg attgccatgc gttcgccaac
     1141 gaggcgattg aacagggtgg atttaccgac attgggtcgg ccgactagtg caacaatggg
     1201 tttcataggg tttattttac tttatggttt aggggttggt gtgggcgtgc tcgaatttag
     1261 cggagtcagc gtcacttcga tggtgttggg caagatcgac tcaacagcga catttgctac
     1321 tagcacttcc acattggggc tgagttgata tttgccttcg gtcagcccag tcaggtccac
     1381 ggtcacgcga accgattggc gtgtgagtgt ctccaagagc ggcaacggac ccgaaagaat
     1441 aatatctaca ttggagggcg agatctgaac atttaaactt tcatccagcc caaggatttc
     1501 aatattttcg ctggagatag tcacactact ttcaatgggc gacacaccag cttgtacaaa
     1561 aaccgtctgc tcgccaatga cggaaatgcc cgaaggcagg ctaagtttca gacgcgtagc
     1621 aatatcatct tgcgcgttct gcagatcaag cggctgggtt tccaacacgc ccggcaagct
     1681 gttcactaaa tcagggtcgg tggaataaat tgtgaccact ggtggtgaga cagaaatatc
     1741 ggtcaggcga tatccgctcg ccacacgccc gagcgtagtc actttgacgg ctacatcgcg
     1801 ataaccgcct tgctggctga cgggcaacac aatatgaacg ctttcaggcg aaactgtaac
     1861 gccattcacc acctgaccgc gatcatccag cacttcgact ttcaccgctt gatcaaaatt
     1921 ctcgcggatg cgattcaggt caatggacac acgagcgcgg ctcactttct cgacctgaga
     1981 ctgggcgcca gacaccacaa cggatactgg gtccagagtg gaatctccta cttgatatcc
     2041 cacagccact tcacccgcca cacgtaaatc cacatccatc gatttggtga taagcggttc
     2101 cagagagaat acaaaggtct ttggcgaaac agaaacgatc tgcaccgggc gagcattgac
     2161 ctgtatttgc aaatccaccg tgtgctcacc ggcactgagt ccagagacat caagaatagc
     2221 gcggacaatg cttgggtcgt catcaatcaa tgcccatact gagcttgggg cgcgcaaagt
     2281 aacagagact tcttttgcaa ttggctcact gaaaacaaga ttgctcgact gaccaatcac
     2341 ttctactgag atcgggttat ctaaagcacg ggtttcatcg gggtccgctg aggtaaccga
     2401 agagatccac acggcaacag ccaatgcgaa tgcccataaa aaagtacggt aattctcaag
     2461 gatccaacga accatgttct acttttcttc cttccggctt tttttgccgg aaaatagctt
     2521 accgataaaa ccaattctct gctctgcacc gctcgggcgg aaaaacgccg tcagaatatt
     2581 ttcaaggcgt tccgggtcga ggcggcggag cattctacca gaatgtgcaa tggaaatggc
     2641 gccggtctct tctgaaacta cgatggcaat cgcatcactc acttctgaaa tccccaacga
     2701 ggcacgatgt cgcaatccca tttgacgttc aggagtgcgg ttcaaaatgc cgctggcaga
     2761 taacggcagc acacaagatg ctgccacgac ttttgaatcc actacgatca cgccgccatc
     2821 atgcaaaggc gtgttcgggt aaaagatctg caaaaata
//
LOCUS       DAOQFT010000321         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21393_length_2857_cov_6.663098, whole genome shotgun sequence.
ACCESSION   DAOQFT010000321 DAOQFT010000000
VERSION     DAOQFT010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21393_length_2857_cov_6.663098"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLR93_08680"
     CDS             complement(<1..514)
                     /locus_tag="PLR93_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27081.1"
                     /translation="MFPAKTLFFTAPRQVEVQESHLPPLKEDEVLVETVCSAISAGTE
                     MLVYRDQFPQLKDSHDAVSSELKYPLAYGYACVGKVREIGKVVGKEWQDRLVFSFQPH
                     TSHFVSKPENLFPIPNSLKPENACFLPNMETAVNLVQDGAPILGERVLVLGQGIVGLL
                     TASLLHEFPLE"
     gene            complement(571..960)
                     /locus_tag="PLR93_08685"
     CDS             complement(571..960)
                     /locus_tag="PLR93_08685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH27082.1"
                     /translation="MQERRKEERKNLVAYTQVYDLYGGNLLGYLGDLTISGAMIISEK
                     AMKPDTEITLAIELPEMPGIKAIRMSLLTRVAWCEQDLSPQYFNVGFEFKEITPDQKG
                     LIEAIIKNYEFRRDVPKYPTRGAPPKQ"
     gene            1037..1762
                     /locus_tag="PLR93_08690"
     CDS             1037..1762
                     /locus_tag="PLR93_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNH27083.1"
                     /translation="MRWIYLSPHLDDAALSAGGWIYDQTQAGNKVEIWTFLCGYPPSK
                     ELSMFARILHMQWGMPTARQVIRMRRAEDKAAATILGAKVRHFNFLDCIYRRDVEGNW
                     LYTDVFVDPHPSEANLPAEIASAISARLKSDDKLICQFGVGKHVDHVTVRRAAELLGR
                     PLHYVTDIPYYFNTPEHLTPNTVGMKKTVERVSRDGLSSWKEAILAYKSQISSLFKSQ
                     RAMRAQINQYCSEFKGMPFWTTN"
     gene            1905..2375
                     /gene="greA"
                     /locus_tag="PLR93_08695"
     CDS             1905..2375
                     /gene="greA"
                     /locus_tag="PLR93_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNH27084.1"
                     /translation="MPTSFLTKEGYDKLQEELDHLRTVKRQEVAERLHEAMEGGELIE
                     NAEYEAAKNEQAFVEGRIQELDLLLATAQIIEDNGKGKKHDAIQVGSKVTIKEGNYEA
                     ETFTIVGAAEANPREGKISNESPIGKAILGHKVGDTVKVETPGGTYNVKILKVA"
     gene            2465..>2857
                     /locus_tag="PLR93_08700"
     CDS             2465..>2857
                     /locus_tag="PLR93_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNH27085.1"
                     /translation="MAEYNSLEKIRLQKVEELRAEGVEPYPTRAKRTHTSAQAIAEFE
                     KDETKEVKVTLAGRIRSMRPKGKLCFAHIEDGDGKIQLFLRVNEVGEDRLAFFNKMFD
                     LGDFVEATGIMMRTKAGEPSLQVLDFNLL"
BASE COUNT          758 a          697 c          697 g          705 t
ORIGIN      
        1 tctcaagcgg aaactcatga agcagagaag ccgttagcag accaacgatc ccttgaccca
       61 acaccagcac gcgttcgcct aagattggag cgccatcttg cacaaggttg acggctgttt
      121 ccatgtttgg aaggaagcag gcattttcag gcttaagcga atttggaatg gggaacaggt
      181 tttcaggttt acttacaaaa tgagacgtgt gtggctgaaa cgaaaatacc aggcgatctt
      241 gccattcttt gccaaccacc ttgccaatct cgcgcacttt ccccacacaa gcataaccat
      301 acgccagcgg gtacttcaac tcgctgctaa ccgcatcatg cgagtctttc aactgtggaa
      361 attgatcacg atagaccagc atctctgtcc ctgcgctaat ggcagagcaa acggtttcga
      421 ctaaaacttc atcctctttc aagggaggga ggtgagactc ttgaacttcc acttgccggg
      481 gtgctgtaaa aaacaaagtt ttagcaggaa acatggcgaa agtttaacat aaaaaggtga
      541 cagtcttttc agactgtcac cttttgtttt ctactgcttt ggtggtgctc cacgggttgg
      601 atatttgggg acatctcgcc ggaattcata gtttttgata atggcttcga tcaacccttt
      661 ttggtctggg gtaatttctt tgaactcaaa accaacattg aaatattgcg ggcttaggtc
      721 ttgttcgcac catgccaccc gggttaatag gctcatacga attgccttga tcccaggcat
      781 ctcaggcagt tcaatggcga gtgtgatttc ggtatcgggc ttcatggctt tttcactgat
      841 gatcattgcc cctgaaatgg ttaaatcacc caggtagcca agcaaattcc caccgtatag
      901 gtcgtacacc tgtgtataag caactaaatt ttttcgctct tctttacgtc gttcctgcat
      961 aaaacctcat ttcgcacaag tgtaaaggaa ggggtgggca agttccattg ctgttttgtg
     1021 gtaaaaatac cgcaccatgc gctggattta tctttcccct caccttgatg atgccgccct
     1081 ctccgctggt ggctggatct acgaccaaac tcaggcaggc aacaaagttg agatatggac
     1141 gtttctgtgt ggatacccgc ccagcaaaga actttctatg tttgcacgca ttctgcatat
     1201 gcagtggggt atgcctacgg cacgtcaggt gattcgcatg cgccgtgcag aggataaagc
     1261 cgccgctact attcttggcg ccaaggtgcg gcacttcaac tttttagatt gtatctaccg
     1321 ccgcgatgtg gaggggaatt ggctctatac cgatgtcttt gtggacccgc atcccagcga
     1381 ggcaaatctc cccgccgaaa ttgcttctgc catttccgct cgcctcaaat cagacgataa
     1441 actgatctgc caatttggcg ttggcaaaca cgtggaccat gtcaccgtgc gccgtgcagc
     1501 agagttgctt gggcgtccgc ttcattatgt gaccgatatt ccatattatt tcaacacgcc
     1561 cgagcatttg actccgaaca cggttgggat gaagaaaacg gtcgaacggg tgagcagaga
     1621 cgggctttcg tcttggaaag aggcgatttt ggcatacaaa tcccagatct cgtcactatt
     1681 caaaagtcaa cgggcgatgc gggctcaaat caaccagtac tgctccgaat ttaagggtat
     1741 gcctttttgg acgacgaatt agacaaaaaa ggtaatactt ttgggggctt gaaacccact
     1801 ataaatcatg atatagtatg cgcgttttcc gggaagtacc accggaaatt tttaatcatt
     1861 gcatctcggt aggggatgca aaattcattt agcaggacaa accaatgcca accagtttcc
     1921 ttaccaaaga gggttatgac aagcttcaag aggagcttga ccacctgcgc accgttaaac
     1981 gccaggaagt tgccgagcgc ctgcacgaag ccatggaagg tggcgagctg atcgaaaatg
     2041 ctgaatacga agccgctaag aatgaacagg cttttgtcga aggacgcatt caagagctag
     2101 accttttgct tgccacagct cagatcattg aagataatgg caaaggcaaa aagcacgatg
     2161 ccattcaagt cggctcaaaa gtaaccatca aagaaggcaa ttacgaagcc gaaacattca
     2221 ccattgttgg tgcagcagaa gccaacccac gtgaaggcaa gatctcaaat gagtctccca
     2281 ttgggaaggc tattttgggt cacaaagtgg gcgacaccgt gaaagtggaa acccccggcg
     2341 gtacgtacaa tgttaagatc ctcaaggtcg cctaaatagg acgcgcctcg ttgccccgcg
     2401 tcctattttt gggatgcggg gcttttttat ataatgacat acatctcgaa gagagaagat
     2461 aatcatggca gaatacaact cccttgaaaa aattcgtttg caaaaagtgg aagaactccg
     2521 cgctgaaggt gtggagccgt accccacacg tgccaagcgg acccatacca gcgcgcaggc
     2581 aattgccgag tttgaaaaag atgagaccaa agaagtgaaa gtcacacttg ccggtcgtat
     2641 ccgttcgatg cgccccaagg gtaagttgtg ctttgcccac atcgaagatg gcgatggcaa
     2701 gatccagctc tttctgcgcg taaacgaagt gggggaagat cgcctcgcct tcttcaacaa
     2761 gatgtttgac ctcggtgact ttgtcgaagc gacaggcatc atgatgcgca ccaaagcggg
     2821 cgagccttcg cttcaggttc tcgacttcaa cctgctc
//
LOCUS       DAOQFT010000322         2853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21422_length_2853_cov_6.694425, whole genome shotgun sequence.
ACCESSION   DAOQFT010000322 DAOQFT010000000
VERSION     DAOQFT010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21422_length_2853_cov_6.694425"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1992
                     /locus_tag="PLR93_08705"
     CDS             <1..1992
                     /locus_tag="PLR93_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNH27086.1"
                     /translation="QELLFEQAEMIESMPDADKEKYEARLQAIIAVLIRTMISDQLKY
                     IKIARQWFTVEDLREINRRKIGGGKIGGKAAGMLLAMRILKETAPVQIRDRFRLPISY
                     YLGSDVYYNFMSLNDLGGWNGQKYKTEEQMRADYPELCEDFYKGQFPPEVIESLERVL
                     EEAGQRPLIVRSSSLLEDNFGTSFAGKYESIFLPNQGDANECLKALTQAIAKIYASVM
                     NPDALIYRHTKELADYDERIGILIQIVEGERAGRYYFPHGAGVAFSRNLFRWSPQIKQ
                     DEGFLRLVTGLGTRAVDMLADDYPRLVALSHPRLHSSSDVRTIKRYSQQHLDAIDIET
                     NSFVTIPVRDALHTDYDPLRYIAQVEQDGYLAPIRSRLDSTDKLVITFDGLLSRTPFS
                     KSMRAALTLLETHYGSPVDTEFTVEIVNPDSQPDVRITILQCRPQSHIHEANEVQIPS
                     DLETQDIVFSSQTMVPQGVVENIGYVLFVPSEGYFSLETQAERTQLERAIGQLNAALK
                     GQTFIAVGPGRWGTSTPDLGVHVAYSDIYNSRALVELAGEEIGASPEPSFGTHFFQDL
                     MEANIYPLGVFVDEEGTIFKRDFFYSTPNRLSEFISIENPRVVAALKLIAVSDFRPNT
                     HMNLIMEASKSRAVAFLVGKATPIESDDAIVGTAPTSLE"
     gene            complement(2050..>2853)
                     /locus_tag="PLR93_08710"
     CDS             complement(2050..>2853)
                     /locus_tag="PLR93_08710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNH27087.1"
                     /translation="TQGLWAAAMWGPGIAAIITTLFIAKVPFNSLRLNTIGPKRFYLW
                     AWFLPTALTVLSGLFTILFGIGKLDLSFSMIRTALESTPGGSEIPASFVVLSQSLLAL
                     TLAPFLNMLFTMGEELGWRGFLLPRLLPLGQWKAVLWSGLIWGLWHAPVIVQGHNYPG
                     YPILGVFMMIVFCFLLGTIIAWLYINTKSPWVAALAHGAVNAIAGLPVLFLAPGFNMA
                     FGGTLAAPTAWIGMALFIAWLVWTKRFPVQDQTDDAQSTSEVDRSEITK"
BASE COUNT          789 a          671 c          642 g          751 t
ORIGIN      
        1 caggaattgc tttttgaaca ggcagagatg atcgaatcca tgccagatgc agacaaggaa
       61 aagtacgaag cgcgtctgca agctattatt gcggtgttga tccgtactat gatcagcgat
      121 caattgaaat acatcaagat cgcgcgtcag tggtttactg tggaagacct gcgcgagatc
      181 aatcgtcgca agatcggcgg cggaaagatc ggcggtaaag cggcgggcat gttgcttgcc
      241 atgcgtatcc tcaaagagac ggcgcctgta caaatccgcg atcgcttccg tttgccaatt
      301 tcttattatc tcggctccga tgtgtactac aacttcatgt cgctcaatga ccttggtggt
      361 tggaatgggc agaaatataa aaccgaagaa cagatgcgtg cagattaccc cgagctgtgc
      421 gaagattttt acaagggaca atttccacct gaagtgatcg aaagccttga gcgcgtttta
      481 gaagaggcag gtcaacgccc gctgatcgtg cgctcctcca gtttgcttga agataatttt
      541 ggcacatcgt ttgccggaaa atacgaaagc attttcttgc ccaatcaagg ggatgctaat
      601 gaatgcctca aggcactcac gcaagctatt gccaagatct atgccagtgt aatgaacccc
      661 gatgcgctga tttatcgtca caccaaagaa cttgctgatt atgatgaacg catcggtatt
      721 ctgattcaaa tagttgaagg cgaacgagct ggtagatatt actttcccca tggggctggt
      781 gtggcattta gccgaaacct tttccgctgg tctccgcaga tcaaacagga tgaaggcttc
      841 ttgcgtcttg ttactggttt aggtacccgc gctgtcgata tgcttgcaga tgattatccg
      901 cgattagtag cgctcagtca tccgcgtttg cattcgtctt cagatgtccg caccatcaaa
      961 cgctattcgc aacagcacct tgatgcaatt gacattgaaa cgaattcttt tgtaaccatc
     1021 cccgtgcgcg atgctctcca cacggattat gatccgctgc gttatatcgc ccaagtggaa
     1081 caagacggat acctcgctcc aatccgttcg cgcctcgact ctaccgataa actggttatt
     1141 accttcgacg gcttactctc gcggacgcct ttctcaaagt ccatgcgagc ggcattgact
     1201 ttgcttgaaa cacactatgg ttcgcctgtc gatacagagt tcaccgttga aatagtaaac
     1261 cctgatagcc agccagatgt ccgcattacg attttgcagt gtcgtccgca aagtcatatt
     1321 cacgaagcga atgaagtaca gattccaagt gaccttgaaa cacaagacat cgttttctcg
     1381 tcacaaacca tggtgccaca aggggttgtg gaaaatatcg ggtatgtttt gtttgttcct
     1441 tcagaaggtt atttcagcct tgaaacacaa gcagagcgta cccaactgga acgcgccatt
     1501 ggtcaactca atgcggcgtt gaaaggacaa acctttatcg cagtaggacc tggacgctgg
     1561 ggaacatcga caccggattt gggtgtgcat gtggcgtaca gcgatatcta taactcgcgt
     1621 gccttggttg aattggcggg agaagagatc ggagcatctc ctgaaccttc tttcggcacc
     1681 catttcttcc aggaccttat ggaggcgaat atctatccgc ttggtgtatt tgttgatgaa
     1741 gaaggaacta ttttcaagcg tgactttttc tatagcaccc caaatcgact ttctgaattt
     1801 atctctattg aaaatccacg agttgtcgct gctttgaaat taattgctgt ttcagacttt
     1861 cgtccaaata ctcacatgaa tttgattatg gaagcaagca aaagtagagc tgtcgctttt
     1921 ctagttggaa aagctactcc aattgaaagt gatgacgcaa ttgttggaac agcaccgaca
     1981 agtttggaat aaaaaaatgg gcgactcatc gtcgcccatt ttttataatt acaaaaaaga
     2041 acaagtgttc tattttgtaa tttcactcct atcaacttct gatgtcgatt gtgcatcgtc
     2101 tgtttggtct tgtactggga aacgtttcgt ccacaccaac catgcaatga acaaagccat
     2161 gccaatccat gcagttggtg ctgcaagtgt tccaccgaat gccatattga aaccaggtgc
     2221 caggaacaat acaggcaacc ctgcaattgc attgactgct ccatgtgcaa gcgctgcaac
     2281 ccatgggctt ttggtattga tatacaacca tgcaatgatc gtcccaagca agaagcaaaa
     2341 tacgatcatc atgaacacac ccagtattgg atagccagga tagttatgtc cttgtacgat
     2401 cacaggtgca tgccacaagc cccatatcaa accactccat aaaacggctt tccattgtcc
     2461 gagtggcaac aatcgcggaa gtaaaaatcc acgccagcct aattcttctc ccatcgtgaa
     2521 tagcatgttt aaaaatggtg caagtgttaa tgccagcaat gattgagata agacaacaaa
     2581 gcttgctgga atttcacttc cacccggcgt agactctaat gcagttcgta tcatgctgaa
     2641 gctcaaatct aatttgccta ttccaaatag aatagtgaag agtccactca gcacagttaa
     2701 tgcagtgggc agaaaccatg cccaaagata aaagcgtttt ggaccaatcg tattcaaacg
     2761 caaactattg aatggtactt tggcaatgaa caaagtagtg atgatggctg caattcctgg
     2821 accccacatt gcagcagccc ataaaccttg agt
//
LOCUS       DAOQFT010000323         2846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21499_length_2846_cov_6.080975, whole genome shotgun sequence.
ACCESSION   DAOQFT010000323 DAOQFT010000000
VERSION     DAOQFT010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21499_length_2846_cov_6.080975"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLR93_08715"
     CDS             <1..953
                     /locus_tag="PLR93_08715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH27088.1"
                     /translation="VDSNSIRFDFAGSGINIRVPKRHALRVIRRDEFDHWLAKQVESK
                     GIEIRQGVTVRKISPDANGVTVETDQETLRATLVVGADGSNGITRRSIFPNEPIYTAR
                     VLEVLTPVSPVSSGSTTPRNQTALFDFFPVPDNIAGYVWDFPTQIKGMEMRCWGIYDT
                     NLLASQKRPALKDPLAKEMQRHGFDLNEYEIKGHPIRWYSPGNKASVSRVLLVGDAVG
                     ADVIFGEGISMALGYGNLAAKEIADSFQRGDFSFRRYKRRLERSGLGQTLFARWLIAQ
                     IIYNLKWRWFQILLWRIVKPIVLFVAWVFVLNWSQRDLRY"
     gene            983..1753
                     /locus_tag="PLR93_08720"
     CDS             983..1753
                     /locus_tag="PLR93_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH27089.1"
                     /translation="MGTADLHVHSIYSRDATTTVRAILKQASSVGLDIIAVTDHDEIR
                     GSLEAQQLAPQYGIEVISGAEVSTKDGHLVALFIKDLPPAGMSLTDTLLHIGDQGGLA
                     IIPHPFNNLPNSLTLEAVFTAMENPTAKASLKGMETHNFGTQNFDKMARKLSVFLPFS
                     KISSSDAHIYWAIGAGRTEFPGKTTQDLRDAIENNKTVPLLYEGESSAKALLSWARRI
                     TMRRFGYASDTSSATEDINTRQMSDSLIRRIQKKESSS"
     gene            complement(1764..2522)
                     /gene="recO"
                     /locus_tag="PLR93_08725"
     CDS             complement(1764..2522)
                     /gene="recO"
                     /locus_tag="PLR93_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNH27090.1"
                     /translation="MTDFRSFRASAVVLRHADWGEADRILTLYTRDQGIVRALAKGAR
                     KVTSRKAGHLQPFTHVTVQLAKGRDLLIITQVETINAYLPLHDDLIKTGYAAYIIELL
                     LRFSYEDEGANPAIFRLVTETLDRLEKETDAWLPVRYYEMRLLDAVGFRPQLFQCANC
                     SREILPEDQYFSFTAGGVICPRCGEGIPNLAHISLEMLKYLRHFQRSSYKEASKARPS
                     LEVQKEAEVLMQGYFTYLLERELNTPGFLKKVKT"
     gene            complement(2565..>2846)
                     /locus_tag="PLR93_08730"
     CDS             complement(2565..>2846)
                     /locus_tag="PLR93_08730"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNH27091.1"
                     /translation="TGVNVENAAYPQTMCAERVAIFKAVSEGETEFEVIAVVTNNGGS
                     PCGGCRQVMAEFGLDAIVLFGNGQGELTHETTVHDLLPEAFTPKHLVTE"
BASE COUNT          773 a          698 c          657 g          718 t
ORIGIN      
        1 atgtggattc aaactcgatc cgctttgatt tcgcaggcag cggcattaac atccgtgtgc
       61 cgaaacgcca cgccctgcgc gtgattcgcc gcgacgagtt cgatcactgg ttggcaaagc
      121 aagtcgaaag caagggcatc gagattcgcc aaggtgtgac ggttaggaaa atctctccag
      181 acgccaatgg cgtcaccgtt gagaccgatc aagagaccct acgtgctacc ctcgtcgttg
      241 gcgcagatgg ctcgaatggc attacacgcc gcagcatttt cccaaacgag ccgatttata
      301 cagcccgcgt actggaagta ctaacgccgg tttcgcctgt ttcaagtggc tccacaacac
      361 ctagaaacca gacagctttg tttgactttt ttccagtccc tgataatatt gcggggtacg
      421 tttgggattt tccaacccaa attaaaggta tggaaatgcg ttgctggggc atctacgata
      481 caaatctgct tgcaagccag aaacgcccgg cacttaaaga cccgcttgca aaagaaatgc
      541 agcgacatgg ttttgatctg aatgaatatg aaatcaaggg gcaccccatt cgttggtaca
      601 gcccggggaa taaggcttcg gtttcaaggg ttttactggt aggagatgca gtaggtgccg
      661 atgtgatctt tggcgaaggc attagtatgg cattagggta tgggaacctc gccgccaaag
      721 agatcgcaga ttcgttccag cgtggtgact tttcgtttcg caggtacaag cgtcggctgg
      781 aaagaagcgg gttgggtcaa acgttatttg cacgttggct catcgcgcaa attatttata
      841 acctcaaatg gcgctggttc caaatcctgc tttggcgcat cgtgaaacca atcgtccttt
      901 ttgtggcatg ggtttttgta ctaaattgga gtcaaagaga cctccgatat tgaacatata
      961 aaagaaaaga aagtaacagc acatgggcac cgcagacctt cacgttcact ccatctactc
     1021 ccgagacgca accaccactg tgcgagccat cctcaagcaa gcttcaagtg tcgggctcga
     1081 cattattgca gtgaccgatc acgatgaaat aaggggctct ctcgaagcac aacaactggc
     1141 accacaatat ggaatcgaag ttatttctgg ggctgaagtt agcacaaagg acgggcactt
     1201 ggtggcattg ttcatcaagg accttccacc ggctggcatg tctctaaccg ataccttgtt
     1261 gcatattgga gatcagggtg ggcttgccat catccctcac ccattcaata atcttccaaa
     1321 cagccttacg ttggaagccg tattcactgc catggaaaac cccacagcaa aggcatcgct
     1381 taaaggaatg gaaacccata actttggcac acagaacttt gacaaaatgg cgcgtaaact
     1441 ttctgtgttc cttccattct caaaaatatc ttcaagcgat gcccatatct attgggctat
     1501 tggcgccggg cgcacagagt tccctggtaa aaccacgcaa gatctgcgtg atgcaataga
     1561 gaataacaaa actgtgccac tgctctatga aggcgaatca tctgccaagg cgcttttgag
     1621 ttgggcgcgc cgcattacca tgcgcaggtt tgggtatgct tctgatacga gttctgcgac
     1681 agaagatata aatacaaggc aaatgtcaga ttctcttatc cgtagaattc aaaaaaaaga
     1741 gagcagttcg taactgctct cttttatgtt ttgaccttct ttaggaagcc gggggtattt
     1801 aattctcgtt ccaacaaata agtgaaatag ccttgcataa gaacttccgc ctctttctgg
     1861 acctcaagac tgggtcttgc ttttgaggct tctttgtagc tcgaacgttg gaaatgccgc
     1921 aaatatttga gcatttccaa tgaaatatgt gccaggttcg gaatcccctc cccacaacgg
     1981 gggcagatca cccctcccgc cgtaaacgag aaatattgat cttctggcaa aatttcgcgc
     2041 gaacaattag cgcattggaa taattgcggg cgaaacccta cagcatccaa taacctcatc
     2101 tcataatagc ggacgggtaa ccaagcatct gtctcttttt caaggcgatc caacgtttca
     2161 gtcacaagcc ggaaaatcgc cgggttcgcg ccttcatctt catatgaaaa gcgtaaaagc
     2221 aattcaataa tgtatgcggc gtaaccagtt tttatcaagt catcatgcaa gggtaaataa
     2281 gcattaatcg tttcaacttg ggtaataatc agtaagtctc gacctttagc aagctgaacc
     2341 gtgacatggg taaaaggttg taaatgcccg gctttccgtg atgtgacttt tctggcacct
     2401 tttgctaacg cacgcactat cccttggtca cgagtgtaaa gcgtgagaat acggtcagct
     2461 tcgccccagt ccgcatggcg aagcacaact gcagaagcac gaaacgaacg aaagtcggtc
     2521 ataaaagaac atggagaatg gttttgtcca ttctccacaa gtttctattc agtcactaaa
     2581 tgttttgggg taaaggcttc aggtaaaagg tcatgaactg tagtttcatg tgtcaactca
     2641 ccttgcccgt tgccgaacaa tacaatagca tccaagccga actctgccat tacttgcctg
     2701 catccgccgc aaggagaacc gccgttattg gtaacaactg cgatcacttc aaattctgtc
     2761 tcaccttctg aaacagcttt gaaaattgcc acgcgttctg cacacatagt ctgtggatag
     2821 gcagcattct ctacattcac ccccgt
//
LOCUS       DAOQFT010000324         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21565_length_2841_cov_4.502154, whole genome shotgun sequence.
ACCESSION   DAOQFT010000324 DAOQFT010000000
VERSION     DAOQFT010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21565_length_2841_cov_4.502154"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PLR93_08735"
     CDS             <1..1274
                     /locus_tag="PLR93_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNH27092.1"
                     /translation="LLLPIDTAMLIGSVSIKVSPAVNFFGRSFELTSADGSLLAVIYG
                     LAALWFFGTEASGTAHRFVSLGLMIVALLTASFAVEPFLYAALLLEMAAMLVVPLLVP
                     LYQKPRRGVVRFIIYQTLAMPFILFSGWMLAGVEASPGDVGTTIQAGVMLLMGFAFLF
                     AIFPLYSWIPQLMEESSPYATGFLLWALPTFTVIFALGFLDRYTWLRNTPQISNGILF
                     AGIIMVTSGGLFASLQRHLGRILGYASIAETGLLLIALSLKSSEIVNITFLTLIPRGL
                     ELTVWALALSILKRRAFSLQFHDIQGLARKYPIASSSLLLAHLSTAGFPLLAGFPSRL
                     ALWQDMAGQSTAYSLWILLGLIGLLIAGVRTLAVLVMTEESTPWQLSESWVQVLMLAI
                     GVAGLFILGIFPQVLQPYLINLPALFEHLSQ"
     gene            1385..2578
                     /locus_tag="PLR93_08740"
     CDS             1385..2578
                     /locus_tag="PLR93_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27093.1"
                     /translation="MEFEQIVKRLEFIDKQQRETKDTLTALKERLTSFESSVDVVSKQ
                     IKALSKQIADVAPVTKRIDQFESVIVKQRTELVKMIEEADKNHTRSERDITKEYRAEF
                     TEIQKAIEQLRTTTNLTDIKKQLKERADEIKRVQANVSDFVSKIEEAKRANDDVKHSL
                     MATEEARKNDLKRIADLQGEIAAVRKRIDEHRDRTTIQADSIRAVETRMNELLVSEAE
                     RKQAQTVFIEQQSIAQIDRERAFKDWQEKFAEFIKEAEGLDAQIQKMDEALRGAKKAQ
                     DTYVDLNTRLERRINEVTEMQRLAEERLRQEWISFKADDQKRWTGYSLSSEEAFRDIR
                     KEVSKYETSITALNEVSQIMQDQIHQTTDASEKQLQELMNVVHEWMVSYQRIMGHGKK
                     ATKKA"
BASE COUNT          753 a          766 c          649 g          673 t
ORIGIN      
        1 cccttctgct tcccatcgat acggccatgc tgatcggctc ggtttccatt aaagtctcgc
       61 ctgccgtcaa cttcttcggc cgtagtttcg agcttacatc agccgatggt tccctattgg
      121 ccgttattta cggactggca gctttatggt tctttggtac agaagcctct ggcaccgccc
      181 accgtttcgt ctctctaggg ctgatgatcg tcgcgttgtt gactgcctcc ttcgctgtcg
      241 agccatttct atatgctgcg cttcttttgg aaatggcagc catgctggtt gtaccgttgc
      301 ttgttccgct ctatcaaaaa cctaggcggg gcgtggtccg cttcatcatc taccaaacgc
      361 ttgccatgcc tttcattctt ttctcaggct ggatgcttgc cggtgtggaa gccagccccg
      421 gtgatgtcgg aacgaccatc caggctgggg tgatgcttct aatggggttt gccttccttt
      481 ttgcgatctt tccgctctat agctggatcc cacaattgat ggaagagtca tccccatatg
      541 ccaccggctt tttgctctgg gcgctgccca ccttcacggt tatttttgcc ctgggttttt
      601 tggatcgata cacatggcta agaaacacgc cccagatctc aaacggcatc ctttttgcag
      661 ggatcatcat ggtgacaagc ggcggtctct ttgcctccct tcaacgtcat cttggccgca
      721 tcctcggtta cgcctccatc gccgaaacag gtctgctcct tatagctctg agcctgaaat
      781 catccgagat cgtcaacata acctttctca ctctcattcc acgcggattg gaattgaccg
      841 tttgggcgtt ggcactttcc attctaaagc ggcgcgcttt ttccctgcaa ttccacgata
      901 tccagggact tgcccgtaaa tatcccatcg caagtagttc attgcttctg gcgcatcttt
      961 ccacagcagg atttccccta ctggcaggct ttccatctcg gctcgcactc tggcaggata
     1021 tggctgggca atctaccgcc tattcgcttt ggatattact gggtttgata ggccttttga
     1081 tcgcaggcgt acgaacgctt gcagtacttg tcatgaccga agaaagcact ccatggcaac
     1141 tcagcgaatc atgggtacaa gtattgatgc tggcaatcgg cgtggcaggt ctcttcattc
     1201 tgggtatttt tccgcaggtg ttacaaccct atctaatcaa cctgcccgca ctttttgaac
     1261 acctgagtca ataagcctct tttgcaaaag ggtataattt aaactggaat tcatcccaac
     1321 aaatctaatg tattcgtgaa acatcagcct ttacattctt tcatttattt attctggagt
     1381 ctccatggaa tttgaacaga tcgtcaaacg tcttgagttc atcgacaaac agcagcgtga
     1441 aaccaaggat actcttaccg cgcttaaaga gcgcctgact tcctttgaat cctcagtgga
     1501 tgtggtctcc aaacagatca aggcactaag caaacaaatt gcagatgtgg ctccggtcac
     1561 caaacgcatt gaccaatttg agtcggtgat cgttaaacag cgtacagaac tcgtcaaaat
     1621 gatcgaagag gctgacaaga atcacacgcg cagtgaacgc gatatcacaa aggagtaccg
     1681 cgccgaattc accgagatcc aaaaagccat tgaacaactc cgcacaacca ccaacctgac
     1741 cgatatcaag aaacagctaa aagaacgggc tgacgaaatt aaacgcgttc aagccaacgt
     1801 ttcagacttc gtctcaaaga tcgaagaagc caaacgggcc aatgatgatg tcaaacattc
     1861 attgatggcc acggaagagg cacgtaaaaa cgatctaaaa cgcattgccg acctgcaagg
     1921 cgagatcgca gccgtacgca aacgcatcga tgaacatcgc gaccgcacca ccatccaggc
     1981 agacagcatc cgcgcagtgg aaacccgcat gaatgagctg ctggtctcgg aagccgagcg
     2041 caaacaagcc caaacggtct tcatcgaaca gcaaagcatc gcacagatag accgtgaacg
     2101 cgccttcaag gactggcagg aaaagtttgc cgaatttata aaagaagccg aaggcctgga
     2161 tgcccagatc caaaaaatgg atgaagccct gcgcggagcg aaaaaagcac aggacaccta
     2221 cgttgacctt aacacccggc tcgagcgacg catcaatgag gtgaccgaaa tgcaacgcct
     2281 cgccgaagaa cgtttgcgcc aggagtggat cagtttcaaa gccgacgacc agaaacgctg
     2341 gaccggttac agcctttcct ctgaagaggc cttccgcgat attcgtaaag aggtcagcaa
     2401 atatgaaacc agcatcacag ccctaaatga agtctctcag atcatgcaag accagatcca
     2461 ccaaaccaca gatgcctctg aaaaacaatt acaggaatta atgaacgtgg ttcacgaatg
     2521 gatggtttct tatcaacgca tcatggggca tgggaagaaa gcaacgaaaa aagcttaacc
     2581 ccaaagccgg accaaaaatc aacgaacaaa aaagtggaga tcaaacggcc tccacttttt
     2641 tgttttgaaa gtgtggttaa attaaagtta tgagagtcat tggcacagca ggacacgtag
     2701 atcatggaaa atccacatta atcgaagccc tcaccggcac acaccccgac cgcctcaaag
     2761 aagagaaggc ccgcgagatg accattgaac tgggcttcgg ctggatgacc cttcccaatg
     2821 gcgaggagat cggcatcgtc g
//
LOCUS       DAOQFT010000325         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21630_length_2835_cov_4.979856, whole genome shotgun sequence.
ACCESSION   DAOQFT010000325 DAOQFT010000000
VERSION     DAOQFT010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21630_length_2835_cov_4.979856"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1387)
                     /locus_tag="PLR93_08745"
     CDS             complement(80..1387)
                     /locus_tag="PLR93_08745"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNH27094.1"
                     /translation="MNFPLSVLIVPSAEHPWTYLVFLVIGIAFGYTLEMSGFGDSRKL
                     AAQFYFTELTVLKVMFTAIVTAMVLLFGAVGLGFLDFNQVWVNPTYLSSGILGGLIMG
                     VGFIIGGFCPTTSLASASTGKIDGMFFMLGGFVGAFIFGETEKYFDAWYNYSGYYGRL
                     TLDQVFNLPVGVVVLLVILMALFMFWGAEQLERLIGKKDMSKEPKIRLAGAGALLVAA
                     IAVVAIGAPSLADRYNKLTFTRTETVEQLNADPIINTYVYTADQMLEKRLVFITPAEA
                     YKAKYNQAMNPIYLDVRSEADYNLYHIEDAVNVPLERITEIVPMLLSEPPTNSVFIVM
                     SNDEAAAIQAWKLLSASSVSNVYILEGGVNNWIAFFGKDDTTFTAQPNPDAGDDQLAY
                     IFPSALGSRYESCSPNPVEYEALEFEAKIKLQLKRDKSGGGCG"
     gene            complement(1398..1943)
                     /locus_tag="PLR93_08750"
     CDS             complement(1398..1943)
                     /locus_tag="PLR93_08750"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNH27095.1"
                     /translation="MTTQTKKSVFNRPEKPYVHPYFGGLVLGIILFLAFFLTGNGLGS
                     SGATSRIDAAIVDAISPSHVDNTPYLLKMAGGDKNPFDEWVVPVFFGALIGGFASGFF
                     NGRLKFMTTKGPNISDRTRWLMAFLGGVIFLYGARMARGCTSGQALSGGATLSAGSWV
                     VMFAIFGGAYGLAYFVRKLWN"
     gene            complement(2019..>2835)
                     /locus_tag="PLR93_08755"
     CDS             complement(2019..>2835)
                     /locus_tag="PLR93_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNH27096.1"
                     /translation="PANDKMYVFGSSRVSWVDWLGALAFAGSLLGVLGHGTLRYIAGR
                     KQPKGHERTERVYMYDAYRRFWHWLQTFAIVILLFTGLIIHRPDVFGAFSFRGMVTIH
                     NILAVILVINALLSVFYHIATDRMQEYIPRPYGFFDDAMVQAKYYISGIFKGEPHPFE
                     KTPTRRMNPIQHATYFGILNVLLPLQMVTGALMWGVQKFPTFANALGGLPFLAPFHSL
                     VAWLFATFILVHVYMSTTGATPLEATRAMITGYEEVEVHEVHEEPKNEKKGKK"
BASE COUNT          792 a          676 c          721 g          646 t
ORIGIN      
        1 attccaagtg tgcgcatagc atcaaaaaaa catccctgaa gtaaatgctt cagggatgta
       61 atatttgctt gcgatgagct tatccgcaac ctccgccaga tttatcgcgt ttcaattgca
      121 acttgatctt ggcttcaaat tcaagggctt catattccac tgggtttggc gagcaagact
      181 catagcggct gcccaaggca gaggggaaga tataggctaa ctggtcatct ccagcatcgg
      241 ggttgggttg agccgtgaaa gtcgtgtcat ccttgccaaa gaaagcgatc cagttgttga
      301 ctccgccttc cagaatgtag acattcgata cactggatgc agagagaagc ttccaggctt
      361 gaatggcagc ggcttcgtca ttgctcatga cgatgaagac tgaatttgtg ggtggctcag
      421 agagcaacat aggcacaatc tctgtgatac gctctagtgg cacgttgacc gcatcttcga
      481 tgtgatacaa gttgtaatct gcttcggaac gcacgtccaa atagatcggg ttcatcgcct
      541 gattgtattt tgctttatag gcttcagcag gcgtaatgaa caccaaacgc ttttcaagca
      601 tttggtcggc ggtgtagaca taggtattga taatcgggtc tgcattcaat tgctcgactg
      661 tttcagtgcg agtgaaggtt aacttattgt agcggtctgc taatgacgga gcaccaatgg
      721 caacaacagc aatagctgct acgaggagtg caccagcccc agcaaggcgg atcttcggct
      781 ctttactcat atcttttttg ccgatcagtc gttcgagctg ctctgcaccc cagaacatga
      841 agagcgccat caaaattacg agcaatacaa ccacacctac aggcaggttg aaaacctgat
      901 ctaatgtgag gcgaccgtaa tagccagagt agttgtacca agcatcgaag tatttttcgg
      961 tttcaccgaa aataaatgca ccaacaaaac caccaagcat gaagaacatg ccatcgatct
     1021 tgccagtgga tgcggacgcg agcgaggtag tggggcagaa gccgccaatg atgaagccaa
     1081 cacccatgat caaaccgcca agaatgccgg aggaaagata cgtagggttg acccatactt
     1141 gattgaaatc caaaaagcca agcccaacgg caccaaacag caagaccatg gctgtgacaa
     1201 tggcggtgaa catcactttg agcacggtca attcggtgaa gtagaactga gccgccagtt
     1261 tgcgagagtc accaaagcca gacatttcaa gggtgtatcc gaaggcgatc ccgatcacta
     1321 aaaagacgag gtaggtccag ggatgttcag cgctcggtac gataaggacg gagagaggga
     1381 agttcattgt attctcctta gttccacaat ttgcggacaa aataagcgag cccgtatgcg
     1441 ccgccaaaga tggcgaacat cacgacccat gaacccgctg agagtgtagc gccacccgat
     1501 agtgcctgac cagaggtgca tccgcgagcc atgcgggcgc catataagaa gatcacacca
     1561 ccgagaaatg ccatcagcca gcgtgtgcgg tctgagatat tgggtccttt ggtggtcatg
     1621 aatttcaaac gaccattgaa aaagccggag gcaaagccgc caatcaatgc gccaaagaag
     1681 accggcacaa cccattcatc gaacggattc ttgtcgccgc cagccatctt caacaaatag
     1741 ggcgtgttat ctacatgcga tggcgagatg gcatctacga ttgccgcatc gatgcgtgag
     1801 gtggcacctg aagaaccaag tccgttgcca gtgagaaaga aagcaaggaa gaggatgatc
     1861 cctaaaacca agccaccaaa ataagggtga acatagggct tttcaggtcg gttgaaaaca
     1921 gattttttag tttgagttgt cattgaaaac tcctataggt aaagcatgaa cctgatccgg
     1981 gctgtgtaca tcatgatata cacagcccgc gttattcttt actttttgcc tttcttctcg
     2041 tttttcggtt cttcgtgcac ttcatgtact tccacttctt catagccagt gatcatggcg
     2101 cgggtcgctt cgagcggagt agcgccagtg gtgctcatgt acacatgcac taggatgaag
     2161 gtagcgaaca accatgcaac tagagaatgg aacggtgcca agaaaggcaa cccaccaagt
     2221 gcgtttgcaa acgtcgggaa tttttgcact ccccacatca aggcaccggt caccatttga
     2281 agcggcaaca aaacattgag aatgccgaag taggttgcat gttgaatggg gttcattctg
     2341 cgtgtgggtg tcttttcaaa tggatgtggt tcacctttga aaatgccgct aatgtaatat
     2401 ttcgcttgca ccatggcatc gtcgaagaag ccgtatgggc gcggaatgta ttcttgcata
     2461 cggtctgttg caatgtgata gaaaaccgag agcaaggcat tgatgaccaa gatcacagcc
     2521 aaaatgttgt gaatggtcac catgccgcgg aaggagaagg caccgaagac atccggtcga
     2581 tgaattatca aaccagtgaa gaggaggatg acgatcgcga aggtctggag ccaatgccag
     2641 aagcggcgat atgcgtcata catgtacaca cgctcggttc tttcatgacc tttgggctgt
     2701 ttgcgtcctg caatgtatct cagcgttcca tgcccaagta cacctaaaag cgacccagca
     2761 aaagccaatg ccccaagcca gtcaacccaa cttacgcggc tggacccgaa gacatacatc
     2821 ttgtcattgg cgggg
//
LOCUS       DAOQFT010000326         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21659_length_2832_cov_7.455528, whole genome shotgun sequence.
ACCESSION   DAOQFT010000326 DAOQFT010000000
VERSION     DAOQFT010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21659_length_2832_cov_7.455528"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="PLR93_08760"
     CDS             complement(<1..1310)
                     /locus_tag="PLR93_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH27097.1"
                     /translation="MKVELKNIHKHFGKTYANKGINLNIPSGSIQGILGENGAGKSTL
                     MKILSGFIQADPGGEIVLDGKSAVMQSPADAIRFGVGMLHQDPLDFPPMKLIDNFLLG
                     MSGKLFPNRKETVQDFNLLAKEYGFNFDPENYVDSLTVGERQQLEIMRLLFLGARVFI
                     LDEPTTGISAAQKIKLFDTLRTFPEQAITTIFVSHKLEDVESLCDRVAVLRAGELVGE
                     FTPPYETKKFVSTMFGKEITLGERQPAKTGDVVLSLEDVSLEDSRVRIDHVNVDIRAG
                     EVIGLAGLEGSGQGMFIRACAGLVRPVGGKVMLKGKNLTGKPYHTFKHEGVNFLPAAR
                     LEEGLVPGLSLTDHFVLAEEPKGVFIDKAYGQKLAEDRIKSFNIRGTPASMIESLSGG
                     NQQRAELALLQTPLSLVLLEHPTRGLDIESVIYIWSKLKDRCKQ"
     assembly_gap    1489..1588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1589..2232)
                     /locus_tag="PLR93_08765"
     CDS             complement(<1589..2232)
                     /locus_tag="PLR93_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH27098.1"
                     /translation="MRNLFARLAVLALALMLVLSACGGQPAATEEAPAATDAAPAATD
                     APAATDAAPAAEPLTFGMLLVGPYNDNGWSQAHYEAGKYVEEQLGAKMLYLDKVNPAD
                     RPGTTPDQLAEELVAQGATLIIFNSDDMKDASTTFAKNHPDVYVIMASGDQVWSDGKA
                     FEAMPNLTNIMGRMEYGKMMAGCAAALASETGKIGYLGPLINDETRRLAASAYLG"
     gene            complement(2364..>2832)
                     /locus_tag="PLR93_08770"
     CDS             complement(2364..>2832)
                     /locus_tag="PLR93_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27099.1"
                     /translation="VDPEVEVDNRLLSPAVRDVAQYSNQSVILFEKQNQLPGQLYGGG
                     PEADWCHYFEQAELARQQGDWETVTHLGDAAFSLSDYPNDPLERFVYIEGYAHQSNWK
                     KAVELSKETYKISKNYVGPLLCRLWGRIERETEASSEQVAALELVRSEFGCLP"
BASE COUNT          708 a          733 c          618 g          673 t
ORIGIN      
        1 tcttgtttgc agcgatcttt gagtttgctc caaatataga tcaccgactc aatatccaag
       61 ccacgagttg ggtgctccaa taagaccagc gatagtggcg tctgtaaaag cgccaattct
      121 gcccgctgtt gatttccacc cgaaagcgac tcgatcatac tggcaggcgt accgcgaatg
      181 ttgaaggatt tgatgcgatc ttccgccagt ttttgaccat atgccttatc aatgaacacg
      241 cccttcggct cttccgcaag gacgaagtgg tcggtcaggc tcaagccggg caccaagcct
      301 tcttccaatc ttgcggcggg aaggaagttg acaccttcat gcttgaaagt gtgatacggc
      361 ttccccgtca aattcttacc tttaagcata accttgccac ctaccgggcg gacgagccca
      421 gcacatgcac gaataaacat gccttgccca gaaccttcca aaccagcaag accaatcact
      481 tctccagcac gaatatcgac attcacatga tcgatgcgaa cacgggaatc ttccaaagag
      541 acatcttcca acgaaagcac aacatcgccc gttttggcag gttgacgttc acctaaagta
      601 atctctttgc caaacatggt tgaaacaaat ttcttggttt cataaggtgg cgtaaattcg
      661 ccaaccagtt cccctgctcg caacacggcg acacggtcgc acaggctttc aacatcttcc
      721 aatttatgtg acacaaaaat tgttgtaatg gcttgttctg gaaatgttcg aagcgtatca
      781 aacaatttta tcttttgcgc agcactaatt cccgtcgtcg gctcatccaa aatgaacaca
      841 cgcgcaccaa ggaataataa acgcatgatc tccaactgtt ggcgttcacc aaccgttaga
      901 gaatctacgt aattttcggg gtcaaaatta aaaccatatt cttttgccag caggttaaag
      961 tcctgtacgg tttcttttct attcgggaaa agttttcctg acatgccaag caaaaagtta
     1021 tcaatcaatt tcatgggagg aaaatcgagc gggtcctgat gcaacatccc caccccaaat
     1081 cggatggcat ctgctggaga ttgcatcact gcagattttc cgtctaaaac gatctcacca
     1141 cctgggtccg cttgaatgaa cccggataag atcttcatca gggtgctttt ccctgcgccg
     1201 ttctccccca aaataccctg tatcgagcca gaaggaatgt taagattaat tcccttattg
     1261 gcataggtct tgccgaagtg cttgtgaatg ttcttgagtt cgactttcat ggtggtgcgg
     1321 gtcctccatg agggtgaaat gaaaatgtag gggcgcgtta tccgcgcccc tacaaaggga
     1381 attacattat tcggctgcgc tcaagccttc cataccttcg agcaattgcg gcaggtacca
     1441 gatctgctgg tctgtagcaa cttcaccgtc ggcgaggtag gcgcttccnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gaggtaggca gaggcagcga ggcggcgggt
     1621 ttcatcgttg attagcggtc cgaggtaacc gatcttaccg gtctcagaag cgagagcagc
     1681 ggcacaacca gccatcatct tgccgtattc catgcgtccc atgatattgg tgaggttggg
     1741 catggcttca aacgccttgc cgtcagacca aacctgatca ccagaagcca taatcacata
     1801 cacatcagga tgattcttgg caaaagtggt ggaagcatcc ttcatatcat cagagttgaa
     1861 gataatgagg gtagcgccct gtgccaccaa ttcttcagcg agctggtcgg gggtggtacc
     1921 ggggcgatct gcagggttaa ctttgtcgag gtagagcatc ttggcgccga gctgttcttc
     1981 gacatactta ccagcttcat agtgagcctg gctccagcca ttgtcgttgt acggaccaac
     2041 gagcaacata ccgaaggtaa ggggctcagc agcgggagca gcatcagtag cagcgggtgc
     2101 atctgttgca gcaggagcgg catccgtggc ggcaggagcc tcctcggtcg ctgcgggctg
     2161 tccaccacag gcagacaaaa cgagcattag tgccaacgcc aaaacggcga ggcgtgcaaa
     2221 taaattacgc atacttcttc tcctttttga aatattttct gtttgacgac tgaccaagaa
     2281 cgaaatcttc tttcgaacct cctttcagtt gtctaaacaa ttttactaaa ctttctatgt
     2341 gcgaagaaag gtacaacgta tttttaaggt aagcacccaa actcacttcg aaccaactcc
     2401 aaagccgcaa cctgctccga agaagcttcc gtttcccgct caatccgccc ccacaaacgg
     2461 cataacaaag gacctacata gttcttagaa atcttgtagg tttctttgga taattctacc
     2521 gcctttttcc agttcgattg atgagcatat ccttctatat atacaaacct ttccagcggg
     2581 tcgttaggat aatcgctcaa ggaaaacgcc gcatcgccca aatgggtaac agtttcccaa
     2641 tcgccctgct gacgtgccag ctcggcctgc tcaaaataat gacaccaatc tgcctccggc
     2701 ccgcctccgt aaagctgacc cggtaactgg ttctgtttct cgaacagaat cacactctga
     2761 ttcgaatact gcgccacatc acgcaccgca ggagaaagca aacgattatc cacttccacc
     2821 tccgggtcga cc
//
LOCUS       DAOQFT010000327         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21672_length_2831_cov_6.176153, whole genome shotgun sequence.
ACCESSION   DAOQFT010000327 DAOQFT010000000
VERSION     DAOQFT010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21672_length_2831_cov_6.176153"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="PLR93_08775"
     CDS             <1..1370
                     /locus_tag="PLR93_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNH27100.1"
                     /translation="SAPPFWTRFLNRKPKEESIPEAPQEPSPEIEQILEEQPPAVVEE
                     EKPKTRGRKKKIVVEEAIVEPVAEVKVEEPESQPSVESQPDGQEQVEIQEEPKQAWWG
                     RVVSLFKIKVQKQDSAFEVVLSEDKEDIPQNGVSSIPDKPADVIMAEIGKMLRERREL
                     ISLTVDEVERHTHLRAVFVKALEEGAFDKLPSTVQTRGMLSNYASFLDLDVDTVLLRY
                     ADALQARRREKYAETPREKIQMQVNPSMPVLRTFIAGDLLFGVIMIAVIVALAIWGVG
                     RLVSSDQQTQVQPTSPSIVDVLGNLSDATASPAVTFVAVDDPAIANAGTAAEGTVTPA
                     GSEVASTANVVVNVYALERVFVRISVDGEVAFEGRLAPRESRFFEANDQVVILTGNGS
                     GLRITYNGEDLGLMGGPGEVVNRVYLITGVVTPTATIPPTPTNTPLTTPTLTPTVTPT
                     GTATP"
     gene            1406..2758
                     /gene="rimO"
                     /locus_tag="PLR93_08780"
     CDS             1406..2758
                     /gene="rimO"
                     /locus_tag="PLR93_08780"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNH27101.1"
                     /translation="MSKNTFHLVSLGCSKNTVDSDSMAQLLTRDGYSFVEDPSKASVL
                     IVNTCGFIGPAKDESYRVLGELAQGKKKNQVLIAAGCLTQRYGVEVAKKVPGIDGIIG
                     TRRWMDIVQVVEDARKGKHPEPIYHLPEAATVGADENGAMRVSVTGPSAYLKIADGCR
                     RPCAFCAIPLIKGTAVSRPVESIIHEARTLRDSGVRELILIAQDSTDYGHDLGMKDGL
                     ATMLEQLTDAVPDMDWIRIMYSYPGYVTDRMIEVMATRKQILPYLDIPLQHAHPKTLY
                     RMKRPSNMDWVHKTLAKMRATIKDLAIRTTFIVGYPGETDEEFQALYDFVEQTRFDRA
                     GAFQFSFEPGTTSAPLGDPVPAEVKQERYERLMELQQNISLQVNQSYVGKTLDVLVEG
                     FDNGISVGRSYRDAPEVDGYVVVEGKAKVGDIVPVRITGAFAYDLTGVLVEQQSLIKL
                     "
BASE COUNT          716 a          621 c          761 g          733 t
ORIGIN      
        1 aatccgcgcc tcctttctgg acccgcttcc tgaatcgcaa acccaaagaa gagtccatac
       61 cggaagcgcc tcaagaaccc tctcccgaaa ttgaacagat tctggaagag caacctccag
      121 cagtagttga agaagaaaaa ccgaaaacac gcggcaggaa gaaaaaaatt gtcgttgaag
      181 aagcaatagt tgaaccagtt gctgaagtaa aagtggaaga acccgaatcc cagccatctg
      241 tggagagtca gccagatgga caggaacagg tcgaaatcca ggaagagccg aagcaagctt
      301 ggtgggggag agttgtttcg ttattcaaga taaaagttca aaagcaagac tcggcgttcg
      361 aggttgtgct ttcagaagat aaggaagata taccgcagaa cggtgtttcg tccattcctg
      421 ataagccggc tgatgtcatt atggcagaga tcggtaagat gctgcgcgaa cgacgtgaac
      481 tcatcagttt gactgtcgat gaagtggagc gtcatacgca tttgcgtgcg gtgtttgtga
      541 aagccttgga agaaggtgct tttgataaac tgccttccac cgtgcagact cgtggcatgc
      601 tttcaaacta tgccagtttt cttgatctgg atgtagatac tgtacttttg cgttatgccg
      661 atgccttgca agcaaggcgc cgtgaaaaat atgcagagac cccgcgcgaa aaaatccaaa
      721 tgcaggtcaa cccctctatg ccggtgttga gaacttttat tgcgggcgat ttgctttttg
      781 gtgtaataat gatcgcggtc attgttgctt tggcaatttg gggcgttgga aggctggtta
      841 gctcagacca gcaaacacaa gttcagccaa cgtctccatc tattgtggat gtgttgggca
      901 atttgtctga cgcgacggct tcacctgcag tgacctttgt tgcggtagat gaccctgcca
      961 ttgccaatgc tggcacagcc gcagaaggca cagttactcc ggcgggttct gaggttgctt
     1021 cgactgcaaa tgtagttgtg aacgtatacg cattggagcg cgtgttcgtt cgtatttctg
     1081 tagatggtga ggttgccttt gagggacgtt tggcaccgcg cgaaagccgt ttctttgagg
     1141 caaacgacca ggttgtcatt ctgactggga atggttctgg gctgagaatc acatataatg
     1201 gtgaagatct tggtctgatg ggcggacccg gcgaagtggt gaaccgggtt tatctcatta
     1261 ccggtgttgt cacgcctacg gcgaccatcc cgccgacacc cacgaatacg ccgcttacaa
     1321 ccccaacctt aacgccgact gtcactccaa caggaacggc aactccgtaa ttcttagttt
     1381 catcaattta ttgtcaggat caaacgtgtc taaaaatact tttcatttag tttctctcgg
     1441 ttgttcaaaa aatactgtcg actctgattc gatggctcaa ttgctgaccc gcgatgggta
     1501 tagttttgtc gaagacccat cgaaggcttc ggtcttgatc gttaatacct gcgggtttat
     1561 tggtccggca aaggatgagt cataccgtgt gctgggggag ttggcgcaag gcaagaaaaa
     1621 gaatcaagtg cttattgcgg caggatgttt gacgcaacgc tatggggtgg aggttgcgaa
     1681 gaaagtgcca ggcattgacg gtattatcgg cactcgccgt tggatggaca ttgtgcaggt
     1741 tgtagaagat gctcgtaagg gtaaacaccc tgaaccaatt tatcatttgc cagaagcagc
     1801 tactgtagga gccgatgaaa atggcgcgat gcgcgtcagt gtgacgggac ccagtgctta
     1861 tctgaaaatt gcggatggat gtcgccgtcc gtgtgcgttt tgtgctattc cgctcattaa
     1921 gggcacggca gtttcgcgcc ctgttgagtc catcatccat gaagcacgca ccttaagaga
     1981 tagcggtgtg cgtgaactca ttttgatcgc gcaagactca actgattatg gtcatgacct
     2041 cggcatgaaa gatggtcttg ccactatgct cgaacagtta accgatgcgg tgccggatat
     2101 ggattggatt cgtatcatgt attcttaccc gggttatgtc accgatcgaa tgattgaagt
     2161 gatggcaact cgtaaacaga ttttgccgta cttggatatt ccgttgcaac atgctcaccc
     2221 taaaacgttg tatcgtatga agcgcccgtc gaatatggat tgggttcaca aaacgcttgc
     2281 caaaatgcgc gcaaccatca aagaccttgc cattcgtacg accttcattg ttggatatcc
     2341 cggtgagacg gacgaagaat ttcaagcctt gtatgatttt gtggaacaaa cccgcttcga
     2401 ccgcgccgga gcttttcaat tctcctttga gcctggcact acctccgcgc ctttaggcga
     2461 cccggttcct gctgaagtga agcaggaacg ttacgaacgg ttgatggaat tgcagcagaa
     2521 tatttcgttg caggtcaacc aatcttatgt gggtaaaacg cttgatgtgc tagtggaagg
     2581 gtttgacaat ggcatttcgg taggacgttc ttaccgcgat gcccctgaag tggatggcta
     2641 tgtggtggtg gaaggaaaag caaaagtggg ggatattgtc cccgtacgaa tcaccggcgc
     2701 ctttgcgtat gatctgacag gagtgttggt tgaacaacag agtttgatca agttataagt
     2761 tgcatggaaa aataaaaatc ggaagtcgct gagacttccg attttttgat tcaagatact
     2821 cttgcgaatg c
//
LOCUS       DAOQFT010000328         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21695_length_2829_cov_7.231074, whole genome shotgun sequence.
ACCESSION   DAOQFT010000328 DAOQFT010000000
VERSION     DAOQFT010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21695_length_2829_cov_7.231074"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PLR93_08785"
     CDS             complement(<1..1013)
                     /locus_tag="PLR93_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH27102.1"
                     /translation="MAKTKVTTPVKKTKPAVKKEAVTKRPAKRSKPSSGKASVFENNS
                     FQQFIEFAPVAFIAIDENSIIQFVNAHAEDLFGYSRKDLIHSNFEMLIPERFRDTHAK
                     QKKAFLKTPQKRAMGSGLDIYGLHQSGKEIPVEVGLNPVTIDGEKYVLVSVSDISTRK
                     EMEVALAASRLKESEEKFRTLVEQNPAIVYISGLHQHIGVTYISPQIQTLGFTQAEWI
                     ADPNLWVEQIHPEDQKRVLKEVEEFALKNQPFRSEYRLLAKDGHVRWFLDEAVDIIDS
                     NGSAIIRQGFMLDISARKDAEETLSSREHYLALLNAITTSIIASQDMDGSIESLVEGL
                     KELL"
     gene            complement(1131..2660)
                     /gene="rny"
                     /locus_tag="PLR93_08790"
     CDS             complement(1131..2660)
                     /gene="rny"
                     /locus_tag="PLR93_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNH27103.1"
                     /translation="MNPIIIILIDILALLVGAVAGYFFHRYQVDRAAKARQERADDIL
                     KAAQTQANMIVKGAQDNATKIIQAAESELKERRIELNRDTERLEKRRSEVDSRYDKIE
                     QREQNLNRRQSQIDKRHNEAEKLFEDQMKKLEEIAQMTQDEARKDLFAAVEKEARGDM
                     ARIIRQIEAEAREEGEKRARKLIADAIQRVASEHVAEVTRAVVTLPSEEMKGRIVGRN
                     GRNIKAFEQAAGVDVIVDDTPDSVTISCFDSVRREIARRALSKLILDGRIHPAHIEKV
                     IDDETRAVEKIINEAGEQAAYDANVSGLHPEILRMMGRLKFRTSYGQNQLAHAVEVSK
                     LAGILAAEIGANVELAKLGGFLHDIGKAMDHNQEGTHAGLGAEFCKRYGVNAVVVNAI
                     AAHHHEVDQETVEAVIAEAADAISGARPGARREDLEAYIKRIRSLEEMSLSFEGVQQA
                     FAIQAGREVRILVKPETIDDLGSTRLARDIAKKIEETMQYPGQIRVTVIRETRATEYA
                     K"
BASE COUNT          650 a          694 c          670 g          815 t
ORIGIN      
        1 agaagttctt ttaacccttc aactagactc tcaatggagc catccatatc ttgggatgca
       61 atgatcgaag tagtaatggc attcaataaa gcaagataat gttcccgaga agataaggtc
      121 tcttcggcat cttttctggc gctaatatcc agcataaatc cttggcggat aatggccgat
      181 ccgttactat caatgatatc tactgcttca tctaagaacc aacgtacatg cccgtctttt
      241 gctagtagtc tgtattctga cctgaaaggc tgatttttta aggcaaactc ttcgacttct
      301 ttcaacactc ttttctgatc ttcaggatga atttgttcta cccacaaatt tgggtcggca
      361 atccattctg cttgagtgaa acccaacgtt tgtatctggg gactgatata agtgaccccg
      421 atatgctggt gaagacccga aatataaaca atagccgggt tttgttctac gagtgtacgg
      481 aatttttctt cagactcttt taatcggctg gctgccaggg caacttccat ctcttttctt
      541 gtagaaatat cagagacaga aaccagaaca tacttttcgc cgtcaatagt gaccggatta
      601 agaccaacct caactggaat ttccttgccg ctttgatgca gaccatagat atccaaccct
      661 gaccccattg ccctcttttg aggagttttg agaaaagctt tcttctgttt cgcgtgcgta
      721 tcccgaaacc tttcggggat tagcatttca aaattgctgt gtattaaatc tttgcgtgaa
      781 tacccaaata gatcttctgc atgtgcgtta acgaactgaa taatactgtt ttcatcgatt
      841 gcaataaatg cgacaggtgc aaattcaata aattgttgaa aggagttatt ctcaaaaacg
      901 gaggctttcc cactagacgg ctttgatctt ttggcagggc gtttcgtcac cgcctctttt
      961 ttgacggcag gcttggtctt ttttacaggg gtcgttacct tggttttcgc catgaccttc
     1021 tctttaataa acagggctgg gactataaac taaaaaccca tcccgcaaag tgcttgtatt
     1081 ttagcaccct gtaggatggg ttaaggtatt cgtaactagc ttgtaaggcg ctatttggcg
     1141 tattcggtgg cgcgggtctc gcggatgacc gtcacacgga tctgaccagg atattgcatg
     1201 gtctcttcga tcttcttggc aatgtcacgg gcgaggcgag tggaaccgag gtcgtcgatc
     1261 gtttccggct tgacgaggat gcggacttca cgtccagctt gaatggcaaa ggcttgttgc
     1321 acaccctcaa aggacaagga catttcttcc agcgagcgga tgcgcttaat ataggcttcg
     1381 aggtcttcac gacgggcacc agggcgagcg ccagagatgg cgtcggctgc ttcagcgatg
     1441 acagcttcca cagtttcctg atccacttca tggtggtgtg cggcgatagc attcaccact
     1501 acagcattga ccccatagcg cttacagaat tctgcgccaa gacccgcatg tgtcccttcc
     1561 tgattgtggt ccatagcttt gccaatgtcg tgcaagaaac cgcctaattt ggcaagctct
     1621 acattcgcgc caatttcagc cgcgaggatg cccgctaatt tagagacttc aacggcatgg
     1681 gcgagttgat tctgaccata tgaggtacgg aacttgaggc gacccatcat gcgcaaaatt
     1741 tcgggatgca aaccagagac gttcgcatca tatgcggctt gttcgcccgc ttcgttaatg
     1801 atcttctcta cagcgcgggt ttcatcatcg atcacctttt cgatgtgagc cgggtgaata
     1861 cgcccgtcga gaatcagctt ggaaagtgca cggcgagcaa tttcacggcg caccgagtca
     1921 aaacaagaga tggtcacaga gtcgggcgtg tcgtccacaa ttacatctac accagctgct
     1981 tgttcgaagg ctttgatgtt gcgtccgtta cgaccaacga tacgtccctt catctcttcg
     2041 ctgggcagag tgaccacggc acgagtaact tccgccacat gttcagatgc cacacgttga
     2101 atggcatcgg cgatgagctt gcgggcgcgc ttttcgcctt cttcccgagc ttcggcttcg
     2161 atctgacgga tgatgcgagc catgtcgcca cgggcttctt tttcaacagc agcgaagagg
     2221 tctttgcgcg cttcgtcttg agtcatctgc gcgatctctt cgagcttttt catttgatct
     2281 tcgaacaatt tctcggcttc gttatggcgc ttgtcaattt gtgattgacg gcgattgaga
     2341 ttttgttcgc gttgttcgat cttgtcgtag cggctgtcca cttcagagcg gcgtttttcg
     2401 aggcgctcgg tatcgcggtt caattcaata cggcgttcct tgagctcact ttcagcagcc
     2461 tgaatgatct tggtggcatt atcttgcgca cctttaacga tcatattcgc ctgggtttgt
     2521 gcagctttca aaatatcatc agcacgttcc tggcgcgctt tggcagcacg gtctacttga
     2581 tagcgatgga agaaataccc cgctacggca ccaactaaaa gtgccagaat gtctatcaaa
     2641 attatgatga tcggattcat gttcattcct catcttcgta ttttgtttcc tctgtgtgcg
     2701 cctcgttcca aatgcgggtg acggtagacg aaaccacaga gtaggggaag ccgcgccttg
     2761 caagatattc ggatagtttc ttacgaaact cgctccattc cagacttttg agtctgggtg
     2821 cccgttttc
//
LOCUS       DAOQFT010000329         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21745_length_2825_cov_5.894224, whole genome shotgun sequence.
ACCESSION   DAOQFT010000329 DAOQFT010000000
VERSION     DAOQFT010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21745_length_2825_cov_5.894224"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..673)
                     /locus_tag="PLR93_08795"
     CDS             complement(161..673)
                     /locus_tag="PLR93_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNH27104.1"
                     /translation="MNVSDAIRLKRAVRKFQDKPLPEDVVYAILNAGRRSQSSKNTQA
                     WKFVAIRDKSILKELSTCGEWAGHLAGAALAVAILTPEPTEKFQIMFDTGQAAAFMQL
                     AAWELGVGSVPASLYEHEKAREILGFPAEWHARIALSFGYPLDEEKLSAPPKKGGRAA
                     LEDVVHWDRW"
     gene            879..1718
                     /locus_tag="PLR93_08800"
     CDS             879..1718
                     /locus_tag="PLR93_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNH27105.1"
                     /translation="MSESTETNQERFKKGMKRIASFLRRGLPFASGVFAALLAFILYN
                     LVIVQPNQLSVEDVNASVASAMGSATPPPAYSAQVYEVIRPSLVLIQTEEKHQDAESD
                     FGLGSGVVVDAFGDILTSRHVIDGATRIKITFADGTESEAQVVSEMPELDLAVIQALD
                     SPPVLYPAILGNPGAMRVGDEAFVVGNPFGLYGSMSSGVISGFDRVFRIPDSNIEIPG
                     MIQFDAAANPGNSGGPLLNRQGHVIGIVTGLTNPTDENFFVGIAFAVPINIAVGGMGS
                     PPY"
     gene            1817..2824
                     /locus_tag="PLR93_08805"
     CDS             1817..2824
                     /locus_tag="PLR93_08805"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNH27106.1"
                     /translation="MEQTNRENQLPMERVLYEVKKVIVGQDHLLERLVVALLARGHIL
                     VEGVPGLAKTMAIKTLADSIGGEFKRIQFTPDLVPADLIGTRIYNQKTGEFNTSLGPV
                     FTNLLLADEINRAPAKVQSALLEVMQERQVTIGRETFKVPNPFLVLATQNPIETEGTY
                     ALPEAQVDRFMLKVLVGYPTQTEEFVIVERMTGAVQAIQRVLTTDELVELQKKVDQVY
                     VDPALIEYVVKLVNATRSPKTVGLDDMEHYITFGASPRASINLVLTAKALAFVRGRTY
                     VLPQDVLDMALDVIRHRLVLSYEALSDEVSSDDILSKILDRIPVPVVPLNEHFNVRAN
                     S"
BASE COUNT          678 a          762 c          685 g          700 t
ORIGIN      
        1 tgagaattca gcttttactt caaccgtctt tttcgtagcc atatcgtttc cctttctcta
       61 ccagcggatt tccttcatta tacctacata gaaacacaat tagcgccggt ctagctccac
      121 agtagctcca cagtgtctcc acataagctc caatcagccc ttaccaacgg tcccaatgca
      181 ctacatcttc caaggctgcc cgtccgccct tctttggcgg ggcagataac ttctcttcat
      241 caagcgggta cccaaacgac agtgcaatgc gggcatgcca ttcagctgga aagcccaaga
      301 tctcgcgtgc cttttcgtgc tcataaaggg aggctggcac cgagccgact cccaactccc
      361 aggcggcgag ctgcataaac gcggcggctt gcccggtatc aaacatgatc tgaaatttct
      421 cggtcggttc gggagtgaga atcgcaacag caagcgctgc gcctgccaag tgtcccgccc
      481 attcgccgca agtggagagt tcttttaaaa tagatttatc gcgaatggca acgaacttcc
      541 aggcctgtgt attcttagat gactgcgaac gtcgccccgc attcaaaatg gcatacacca
      601 catcctcggg caggggcttg tcctgaaact tgcgtaccgc tcttttcaat cgaattgcat
      661 ctgaaacatt catcacaacc tcatcctatt tgaacctggg tatagatact caatactaaa
      721 agtatacatg tcccttttat aattgtgtgt attttgtgaa cacccctttg cactgtaaga
      781 atacaaccat ttctctcatc tttagctaaa ataagcacct tatagtactc aaacaagttc
      841 gcgtccgctc caactacttt agataaggat tcttttgcat gagcgaatcg actgagacaa
      901 atcaagagcg attcaaaaag gggatgaaac gcatcgcttc tttcttacgc cgaggcttgc
      961 cttttgcttc gggagttttc gctgcccttt tggctttcat tctctataac ctcgtcatcg
     1021 tccaacccaa tcaactgagc gtggaggatg tcaacgcatc ggtggcatct gccatggggt
     1081 ctgcgactcc gccaccggca tattcggcac aggtctatga ggttatccgc ccatcgttgg
     1141 tattaattca aacagaagaa aagcatcaag acgctgaatc agatttcggc ttgggaagcg
     1201 gcgtggtcgt cgatgcattt ggcgatatcc tcaccagtcg tcatgtcatt gatggcgcga
     1261 cccgcatcaa gatcaccttt gccgatggca ccgagtctga agcgcaagtc gtttctgaaa
     1321 tgcccgagct agacctcgcc gtcatccaag ccttggattc tccgcccgtg ctatacccag
     1381 cgatattagg caaccccggc gcgatgcgag tgggggacga agcctttgtg gtaggcaacc
     1441 cgttcggctt gtacggctcg atgagttcgg gagtcatctc gggcttcgac cgggtctttc
     1501 gtattcccga tagcaatatt gaaatccccg gcatgatcca attcgacgct gccgccaacc
     1561 ccggcaactc tggcggaccc ttgctcaacc ggcaaggaca tgtgatcggc attgtgaccg
     1621 gtttaaccaa ccccacagac gaaaattttt ttgtaggcat tgcctttgca gttcccatca
     1681 atattgcggt gggtggtatg gggtcgccac cgtattaggt ataaaccgta ggggcgaccc
     1741 gcccggcggg agcaaaacat tcttatctta aggaagggtc gcacttactt aggcgaccat
     1801 aataaggaga ccaagcatgg aacaaacaaa tcgcgagaat caacttccga tggagcgggt
     1861 attgtacgaa gtcaagaaag ttatcgttgg acaagaccat ttattggaac gcttggtcgt
     1921 cgccttgctc gcacgcggac atatccttgt cgaaggtgtg ccgggcttgg caaagaccat
     1981 ggcgatcaaa accctcgccg actccatcgg cggtgaattc aagcgtattc aattcacccc
     2041 agaccttgtc cctgccgatt tgatcggtac gcgtatttac aaccaaaaga caggcgagtt
     2101 caacacgtct ttgggtccgg tcttcaccaa cttgttgctt gccgatgaaa tcaaccgcgc
     2161 gcctgccaaa gtgcaaagtg ccttgctcga agtgatgcaa gaacggcagg tgacgattgg
     2221 acgtgagacc ttcaaagtgc caaatccgtt cttggtgttg gcgactcaga atcccattga
     2281 aaccgaaggg acttatgctt tgcccgaagc ccaagtggac cgtttcatgc tcaaggtttt
     2341 ggtgggctac ccaactcaaa cggaagaatt cgtgatcgtc gagcgcatga ccggtgccgt
     2401 gcaagccatt caacgcgtgc tcacaaccga tgagttggtg gaattacaaa agaaggtaga
     2461 ccaagtctat gttgacccgg cgttgatcga atatgtggtc aagttggtca atgccacacg
     2521 cagcccgaaa accgtggggt tggatgacat ggaacattac atcacctttg gtgccagccc
     2581 gcgtgcatcc atcaatttgg tgttgactgc caaagcgctc gccttcgtgc gcggacgtac
     2641 ctacgtcttg ccgcaagatg tgctcgatat ggcgttggat gtcattcgtc atcgtttggt
     2701 gctttcgtat gaagcgctct ctgatgaagt cagcagcgat gatattttga gcaagatcct
     2761 cgaccgcatc cccgtgcctg tggtgccatt gaatgaacac ttcaacgtcc gcgcgaactc
     2821 ctgag
//
LOCUS       DAOQFT010000330         2823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21768_length_2823_cov_5.679913, whole genome shotgun sequence.
ACCESSION   DAOQFT010000330 DAOQFT010000000
VERSION     DAOQFT010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21768_length_2823_cov_5.679913"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLR93_08810"
     CDS             <1..281
                     /locus_tag="PLR93_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNH27107.1"
                     /translation="DIDKPEERADLVERSPKTHLHKMAAPMLVIQGRNDPRVVAAESE
                     DLVRELKASGKPIELLVFEDEGHDVLKYNNRVTCYNAIADFFAKHLNP"
     gene            285..563
                     /locus_tag="PLR93_08815"
     CDS             285..563
                     /locus_tag="PLR93_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27108.1"
                     /translation="MNLPAILFALVVALLAGALFHAIRGGGGWRLLLHLALSAAGFAL
                     GQGISIWLGYILLQFGVLDIGMGVIGSLVFLGIGDWLSRIKPRNESSV"
     gene            611..1243
                     /locus_tag="PLR93_08820"
     CDS             611..1243
                     /locus_tag="PLR93_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH27109.1"
                     /translation="MDFLKSAIDLFLHLDEYLQTIITTYGAWTYGILFVVIFIETGLV
                     VMPFLPGDSLLFAAGTFAALGSFNVWGLVGLLAVAAVLGDAVNYSIGHYLGDRAYNIK
                     WIKKEYLEQTHAFFEKHGGKAIFLARFVPIVRTFAPFVAGIGKMSYSYFATYNIVGGI
                     SWVTIFTLLGFFFGNIPFVKKNFELVIIAIILISVVPMIYEWWKARRAAK"
     gene            1297..1956
                     /locus_tag="PLR93_08825"
     CDS             1297..1956
                     /locus_tag="PLR93_08825"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNH27110.1"
                     /translation="MNHIELSSKEESLLNQETLAEKMELVVEKLPTDEVTLVEIMDIV
                     GADSLMLVTIFMSLIFLVPVSIPGVSTVFGTGILLIGITHLFARKLWLPKPIANRKLS
                     APKLKEAFKRAIVWLHRLEKVSRPHRLSKLTAEGGMTIFNNLSFILAALLLMAPFGFI
                     PFSNTLPALALIFFALGMMERDGGSVIFGHIANIATIIYFGFLIAGGGWSIGEFIKVL
                     G"
     gene            2001..2363
                     /locus_tag="PLR93_08830"
     CDS             2001..2363
                     /locus_tag="PLR93_08830"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNH27111.1"
                     /translation="MKYLAILFTLFILAIIILADKNALPPFIHAIYDFPNGDKAGHFI
                     LFGLLNFILTLTFLRALPNRTRSRVALSVGLILALAITLEEFSQRYFSSRTFDLFDLT
                     ASYIGVLVGGWIAFRIKK"
     gene            complement(2396..>2823)
                     /locus_tag="PLR93_08835"
     CDS             complement(2396..>2823)
                     /locus_tag="PLR93_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNH27112.1"
                     /translation="ETVQQLEKEYGKDPAKWPRWGELHTATFRNGTLGESGVFLIEDL
                     FNRGPFETNGGQSLVNATGWDLGTSFEVDWLPSMRMIVDFNDLNASLTVHTTGQSGHA
                     YHPHYDDMALFWADVSYYPMWWDQESVIDDAEGHLVLKP"
BASE COUNT          650 a          671 c          649 g          853 t
ORIGIN      
        1 gcgatattga taaacccgaa gaacgcgccg acttggtcga acgctcgccc aagactcacc
       61 tgcacaaaat ggcggcaccg atgctcgtca ttcaaggacg taacgatccg cgtgtggtag
      121 cggcagagtc agaagacctc gtccgcgaat tgaaagcgag tggaaaaccc attgagttgt
      181 tggtttttga agatgagggt cacgatgtgt tgaaatataa caatcgtgtc acctgctata
      241 acgccattgc tgatttcttt gcgaaacatt tgaacccgta aactatgaat ctgccagcca
      301 ttttgttcgc attggtcgtg gcattattag caggcgccct gttccatgcc attcgaggcg
      361 ggggcggttg gcgcctgctg cttcaccttg ctctgagtgc agccggattt gcactcgggc
      421 agggcatcag catctggctt gggtatatct tgcttcaatt cggagtgtta gatattggga
      481 tgggagtaat cggtagcttg gtctttcttg gcatcggtga ctggctgagc cgcataaaac
      541 cacgcaatga aagcagtgta taatctccgc ccgttcgttc aaaaaacatc atgaaagcga
      601 gtaaaaacaa atggacttcc taaaaagtgc gatcgatctc tttttacatc tcgatgaata
      661 tttgcaaacc attatcacca cctacggtgc ctggacctat ggcattcttt ttgtagtgat
      721 ctttatcgaa acgggcttgg ttgtgatgcc cttcctgcca ggagactccc tgctctttgc
      781 tgcagggacc tttgctgcgc tcggctcttt caatgtgtgg ggcttggtcg ggttgttggc
      841 agtcgctgcg gtgttgggcg atgcggtcaa ttactccatc ggtcattatc ttggtgaccg
      901 tgcctacaac atcaaatgga ttaagaaaga gtatttggaa caaactcatg cctttttcga
      961 gaagcatggc ggcaaggcta ttttccttgc gcgctttgtt cctatcgtcc gtacatttgc
     1021 tcctttcgta gctggtattg gcaaaatgtc ttactcttat tttgccacct ataacattgt
     1081 gggcggtatt agctgggtga caatctttac tttactcggc tttttctttg gcaatatccc
     1141 ctttgtgaaa aagaattttg agttggtcat tattgccatc attttgatct cggttgtgcc
     1201 gatgatttac gaatggtgga aggcacgtcg cgccgcgaaa taagctgtag cgttctgaaa
     1261 tcacttttta ctttagaaaa tactgaggat attaaattga accacattga attgtcttcc
     1321 aaggaagagt ctttgcttaa tcaagagact cttgccgaaa aaatggagtt ggttgttgaa
     1381 aaactcccca cagatgaagt gactttggtt gagatcatgg atattgtcgg tgctgatagc
     1441 ctcatgctgg taaccatctt catgtccttg atctttcttg tgccggtttc cattcctggc
     1501 gttagcacgg ttttcggaac gggtattctc ctgatcggta ttactcatct ttttgcaaga
     1561 aagctttggc tgcccaaacc cattgcgaat cgtaaactct cagcacccaa attgaaagag
     1621 gcattcaaac gtgctattgt ttggttgcat cgtcttgaaa aggtcagccg tccacatcgc
     1681 ttgagcaaac tcacggcaga aggtggcatg accattttca ataatttgag cttcattctt
     1741 gccgctcttc tgctcatggc gccttttggg ttcattccgt ttagcaacac ccttcctgcc
     1801 ttggcattga tcttttttgc acttggcatg atggaacgag atggcggctc cgtgattttt
     1861 gggcatatcg ccaatattgc cacgatcatc tatttcggct tcctcattgc aggtggcgga
     1921 tggtctattg gcgaattcat taaagtgctt ggttaactct ttataaggca ggctgggttc
     1981 caagcccagc ctgttttttt atgaaatatc tagctattct ttttacactg ttcattcttg
     2041 cgatcatcat tcttgcagat aaaaatgctt taccaccatt tattcatgca atctatgatt
     2101 ttccaaatgg cgacaaagcc gggcacttca tcctctttgg actcctcaac tttattctca
     2161 ctcttacctt tctccgcgcg cttcccaacc ggacccgtag ccgggttgct ctttcagtcg
     2221 gtttgatact cgccctcgcc atcaccctcg aagaattctc gcaacgttac ttttcctctc
     2281 gtaccttcga tttgttcgat cttacagcaa gttatatagg tgttttagta ggcgggtgga
     2341 tcgccttccg aataaaaaag taggtcacgc ctcaagcgtg acctactttt ttattttagg
     2401 gcttcaacac caaatgccct tccgcatcat caatgacgga ttcttgatcc caccacattg
     2461 ggtagtagga aacgtctgcc cagaacaaag ccatatcatc gtaatgcgga tgatatgcat
     2521 gcccggactg ccctgttgta tgaaccgtaa gagacgcatt gaggtcatta aaatccacga
     2581 tcatacgcat ggagggcaac caatctactt cgaaggatgt gcccaagtcc cagccagttg
     2641 cgttgactag gctttgaccg ccgttggtct cgaaaggtcc gcgattgaag aggtcttcaa
     2701 tcaagaagac accagattcg ccaagtgtcc catttctaaa cgtggcagtg tgtaactcac
     2761 cccagcgcgg ccatttggcg gggtctttcc cgtattcttt ttccaattgc tgaacagtct
     2821 cag
//
LOCUS       DAOQFT010000331         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_21890_length_2815_cov_6.181884, whole genome shotgun sequence.
ACCESSION   DAOQFT010000331 DAOQFT010000000
VERSION     DAOQFT010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21890_length_2815_cov_6.181884"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLR93_08840"
     CDS             complement(<1..215)
                     /locus_tag="PLR93_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27113.1"
                     /translation="MSSQPIPVIEARGTYREVGKQIGEQCKPQIARMMSQLHESLPPG
                     KTWEMLVNESRIYQTHSRAVYPQYMDEL"
     gene            complement(290..1168)
                     /locus_tag="PLR93_08845"
     CDS             complement(290..1168)
                     /locus_tag="PLR93_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH27114.1"
                     /translation="MNSKSFTRLSVLVVLAMLAALIPLQVHAGGVCGGAYTIEKGDTV
                     DKLASMCGTTASAIYATNPGLKEPLTVGQVITIPGANYGVTTTPDAAATPTPTAVVGN
                     ATAMPAAVVNYYTYNYYNYYYANPTVTTSTTYIVQAGDTFATIAARYGLSVSQLWAAN
                     PQLTDINVIYIGQLLYIPANTYYPTAVPSAVATQELKKHVDNSQVPSNAAYATIWLIN
                     KSEADVYISLRTSRADGTNAINEFPVGRQMDVEIPTGWVDYTAYVGGRKFTGGFMVRE
                     GSQHTITFNRSKVVVD"
     gene            1346..2266
                     /locus_tag="PLR93_08850"
     CDS             1346..2266
                     /locus_tag="PLR93_08850"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNH27115.1"
                     /translation="MNTTYQQILDEIYQETLPLLGQGKVASYIPALAEVNPKQYGIAL
                     QTLSGETYQVGDANTLFSIQSISKVFTLAMVFGKVGEALWTRVGREPSGNAFNSLIQL
                     ETENGIPRNPFINAGALVVTDHLLSQTSAPLWDVLGFVRKLSNNPKINFSEVVAESER
                     RSGFRNAALINLMKSFKNIENNVDEVLDVYFHQCALMMNCHDLANAFLFLANGGVNPW
                     NGERILTGSQSKRVNALMQVCGLYDDSGEFAFTVGLPGKSGVGGGIVAVIPNLLSICV
                     WSPELNSHGTSLIGMKALELFTTKTGISIF"
     gene            complement(2267..>2815)
                     /locus_tag="PLR93_08855"
     CDS             complement(2267..>2815)
                     /locus_tag="PLR93_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH27116.1"
                     /translation="LIYSSGPAAFDFVFSRKAGQALEFLHFAFLDGAGEMGYKNHIVG
                     EQAGNVLAVGAAFDGSTGFPFFLAAARQIIRFYGTSCLPAMIRGLQVENIIQPPKGDV
                     HYIAHLGVHPSMQGKGIGTQLVEHLMEQGRNAGKTHTALDVSVLNPRAQVLYERLGFK
                     LTGQRESKLPGVPSHNRLERAL"
BASE COUNT          696 a          644 c          740 g          735 t
ORIGIN      
        1 agctcgtcca tgtattgcgg gtagacggca cggctgtgag tttggtagat acggctttcg
       61 ttcacgagca tctcccaggt tttgccgggt ggcaaactct catgcagttg actcatcatc
      121 ctagcgattt gcggtttgca ttgttcccca atttgtttgc cgacttcacg atatgttccg
      181 cgtgcttcaa taacgggaat gggctggctg gacatacatt ctccttgaat caaaaatgac
      241 ccgctaagtg tatcagaaca cgaagcgggt cattttatgg tcttaacgat taatcaacga
      301 cgactttact gcgattgaaa gtaatggtgt gttgcgaacc ttctcgaacc atgaaaccac
      361 ctgtgaactt tctgccacca acataggcag tatagtccac ccaaccggtc gggatttcaa
      421 catccatttg ccttccaaca gggaattcat tgatggcatt tgttccatct gcgcggctgg
      481 tgcgcaacga aatatacaca tccgcttcag acttgttgat gagccaaatg gtggcatacg
      541 cagcattgct tggaacttgg gaattatcca catgcttttt caactcttgc gtagctactg
      601 ctgaaggaac cgcggtggga tagtaggtat ttgccggaat ataaagtaac tgaccgatat
      661 agattacgtt gatatcggtc aactgcgggt tggcagccca aagctgagaa acgctgagcc
      721 cataccgggc agcgatggtc gcgaatgtat caccagcttg aacaatataa gtagtactag
      781 ttgttaccgt ggggttggcg taataataat tgtagtagtt ataggtatag taattgacta
      841 cagccgcagg catggcggtt gcatttccaa ccaccgctgt gggggtgggc gttgcagccg
      901 catccggtgt agtggtcaca ccatagtttg ctcccggaat ggtgatcact tgcccaactg
      961 tgagcggctc tttcaacccg gggttggtgg catagatcgc ggatgcggtt gtcccgcaca
     1021 tggacgcaag cttatcgacg gtatcgccct tttcaatggt ataggctcca ccgcatacgc
     1081 ctcctgcgtg gacctgaagc ggaatcagcg ctgccagcat tgccagcacc accaaaacgc
     1141 ttaaccgagt aaatgatttt gagttcaaaa ctgcctcctt gcacggtcta ctttaattcg
     1201 tataacccta tgataagcca aaaagagtcg aaaggcaact ggttttgtaa gaaaaattgc
     1261 ttacaaatac ataacatggc tccatttttg cgaaaatagg gcttttgagc gaggcttgat
     1321 aaaattatcc gtacggagaa ctgaattgaa tacaacctat caacaaattt tagatgagat
     1381 ctatcaggag accctgcctt tgcttgggca aggcaaagtt gccagttata tccctgcttt
     1441 ggcagaagtg aacccaaaac aatatgggat cgcgcttcaa accttgagcg gtgagacgta
     1501 tcaagtcgga gatgccaata cgcttttctc tattcagagt atttcgaagg tttttacgtt
     1561 agcgatggtg tttgggaaag tgggcgaggc gttgtggact cgggttggac gcgaaccttc
     1621 gggtaatgcc tttaattcgt tgatccagct tgagacagag aatgggattc cgcgtaatcc
     1681 ttttatcaat gccggtgctt tggtagtgac cgatcatctg ctttcgcaga cgagtgcgcc
     1741 gttatgggac gtgcttggct ttgtgcgaaa gctttccaac aacccaaaga tcaacttcag
     1801 tgaagtggtt gcagaatcag agagacgttc tggctttcgc aatgcggcat tgatcaactt
     1861 gatgaagagt ttcaagaata ttgaaaataa cgtcgatgaa gtcttggacg tttatttcca
     1921 tcaatgtgcc ttgatgatga actgccatga cttggcaaat gcctttttgt ttcttgcgaa
     1981 tggtggcgtc aacccgtgga atggcgagcg gattttgacc gggagtcagt ccaaacgagt
     2041 gaatgcgttg atgcaagtct gtgggttgta cgatgattcg ggggagttcg cgtttacggt
     2101 ggggctgccc ggcaagagcg gagttggagg cgggatcgtg gcggtcatcc cgaacctgct
     2161 ttccatctgc gtttggtcgc cggagttgaa cagccatggc acatccctga tcggcatgaa
     2221 agccttggag ttgttcacca cgaagacggg catttcgatt ttttagttac aaggcgcgtt
     2281 ccaagcggtt atgagacggc acaccgggca acttcgactc gcgttgaccg gttaacttaa
     2341 agcccagccg ttcatacaag acctgcgcac gcgggttgag cacggagaca tccaatgcgg
     2401 tgtgagtctt gcctgcgttg cgtccctgct ccatgagatg ctctacgagt tgggtaccaa
     2461 tgcccttgcc ctgcatggac gggtggacgc cgagatgcgc gatgtaatgt acatctccct
     2521 tgggcggttg aataatgttt tccacttgta agccgcgaat catggcaggc agacaggacg
     2581 tgccataaaa acgaatgatc tgtcttgctg cggcaaggaa gaaaggaaag cctgttgacc
     2641 catcaaatgc ggctccaact gcgagcacgt ttccggcttg ttccccaaca atatgatttt
     2701 tgtatcccat ttcaccggcg ccatctaaaa atgcgaaatg taaaaactca agggcttgac
     2761 cagctttgcg gctgaacaca aaatcaaagg cggcgggacc ggaactataa attag
//
LOCUS       DAOQFT010000332         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22020_length_2806_cov_6.838604, whole genome shotgun sequence.
ACCESSION   DAOQFT010000332 DAOQFT010000000
VERSION     DAOQFT010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22020_length_2806_cov_6.838604"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..798
                     /locus_tag="PLR93_08860"
     CDS             91..798
                     /locus_tag="PLR93_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH27117.1"
                     /translation="MPEKILIVEDEIALQETLAYNLKKEGYTVETVGDGRSALEAARK
                     LKPDLIVLDIMLPEMDGFEVARTLRKEMTTGILMLTARDDEIDRVVGLEVGADDYLTK
                     PFSMRELIARVKAQLRRARLLREEMGKSVEETPHETLTFDNLIINLTRREVTLNGTPV
                     QLKPKEYELLLFLAEHRRQMLSREFILERVWGWDYIGDSRTVDVHIRWLRQKIEESSS
                     EPKRIVTVRGGGYRFEG"
     gene            848..2086
                     /locus_tag="PLR93_08865"
     CDS             848..2086
                     /locus_tag="PLR93_08865"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH27118.1"
                     /translation="MNQFAYATAIVFLIIAAWFAWKYYNLRKNVNQLAKSLHDPKIPL
                     PHLREIEELLSMIESRKATFSAELFSLQTENERLATVLEQLTDGVLIADADGRVQFAN
                     PAASKLFGTHNPTQQSVAQVVRSHQLIEAWKRCQQTRQLQIEAVEIATRKQYLQIIVI
                     PDRHEGGSLLLAQDLTQVRKLETVRRDFISNVSHELRTPLASLKALTETLQGGALADP
                     EAGPRFLDRIHTEVDALTQMTQELLDLSRIESGQVQLNFEMISPRKLIYSAADRMKAQ
                     VERAHLKLDVKCEDGLQNIKADKNRLEQVLVNLIHNAVKFNKPGGSITLSAEPTSGGV
                     RCAVRDTGVGVPMESLNRIFERFYRVDSSRTGSGTGLGLSISKHIIEAHGGKIWAESE
                     EGRGSTFFFIVPLDPSKAKE"
     gene            complement(2049..>2806)
                     /locus_tag="PLR93_08870"
     CDS             complement(2049..>2806)
                     /locus_tag="PLR93_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH27119.1"
                     /translation="ARTAELTVVNADLEKAMRAKDEFMAAMSHELRTPLTGILGGAGA
                     LQGSTDDTLSDKQKKSVNLIERNGHRLLSLVNDLIDYSRLQSGKQILFKSPYALTDIC
                     IAALRSVETDAKRKKQSVSLQSQPEGIIVDTDVTLMKKMLIHLLRNASKFTPEGGEFG
                     ISLTEKSEQHCVEVVVWDTGIGIKKSDIANIFTPFVQLDATLARKYEGTGLGLAIVNK
                     LVELFDGKMDVFSIPGSGSRFVILLPWKGPTEQ"
BASE COUNT          768 a          690 c          659 g          689 t
ORIGIN      
        1 aaaaaagaaa ataaaactgg ctgactttca gttcaagcca ttaacatcac cttaaaagat
       61 cagggattcc tttatggtat agtgccactc atgcctgaaa agatattgat cgttgaagac
      121 gagatcgctc ttcaagaaac ccttgcctat aacctaaaaa aagagggcta taccgttgaa
      181 accgtaggcg atggtcgctc tgcgctcgaa gctgcccgta aactaaaacc agacttgatc
      241 gtgctcgaca ttatgttgcc agaaatggat ggcttcgaag tggcgcgcac attacgcaaa
      301 gaaatgacaa caggcattct gatgctcacc gcccgcgatg acgagatcga ccgtgttgtt
      361 gggctggaag tgggcgccga cgattaccta accaagcctt tctcgatgcg tgaattgata
      421 gcacgcgtca aagcgcaatt acgccgtgcc cgcctattaa gagaagagat ggggaaatct
      481 gtagaagaaa cgccacacga aaccctcacc tttgataacc tcatcatcaa cctcacccgc
      541 cgcgaagtga cattaaacgg cacccccgta caactgaagc caaaagaata tgagcttttg
      601 ctctttcttg ccgaacaccg ccggcaaatg ctgtcacgtg aatttattct ggaacgcgtt
      661 tggggttggg actatattgg cgatagccgc actgtcgatg tgcatatacg ttggttgaga
      721 caaaagatcg aagaaagttc aagtgagccg aaacgtattg ttaccgtccg tggcggcggg
      781 tatcggtttg aaggatgaag tatgaggagc aacctacggc tctctcatta cgtattgtga
      841 cccacgtatg aatcaatttg cttatgcaac agctattgta tttctcatca ttgcggcgtg
      901 gttcgcatgg aagtattaca acctgagaaa aaacgtcaat cagttggcga aatctctgca
      961 tgaccctaaa attccgctcc cccatttaag agaaattgaa gaactgctta gcatgattga
     1021 gtcgcgcaag gcaactttct ctgctgagct tttctctctg caaacagaaa atgagcgcct
     1081 tgctacagtc ctcgaacagc ttaccgatgg cgtgctcatt gccgatgccg atggccgggt
     1141 gcaatttgca aaccctgcgg caagcaaact ctttggcact cacaacccca cacaacaaag
     1201 cgtggcacag gtggtgcgca gtcatcaatt aattgaagcg tggaagcgct gccagcaaac
     1261 ccgtcaactt caaattgagg cagtagaaat cgccacgcgc aaacagtatt tgcagatcat
     1321 cgtcattcca gaccgccacg aaggtgggag tttattgctc gcgcaagacc tcactcaggt
     1381 gcgcaaactc gaaaccgtac ggcgggactt tatctcgaac gtctcccatg aattgcggac
     1441 gccgcttgct tcgctaaagg cgctcaccga gacattacaa ggcggcgctc tcgcggaccc
     1501 ggaagctggt ccgcgtttct tagaccgaat ccacactgaa gttgacgcgc tgactcagat
     1561 gacccaagaa ctgctcgacc tttctcgcat tgagtctgga caagtgcagc tcaactttga
     1621 aatgatctcg ccgcgtaaac tcatctactc tgccgcagac cgaatgaaag cacaagtgga
     1681 acgtgcgcat ttgaaattag atgtaaagtg tgaagatggg ttgcaaaata tcaaagccga
     1741 taaaaatcgg ttggaacagg tgttggtcaa cctgattcac aatgcggtca aattcaataa
     1801 gcctgggggg agtatcactc tgagtgcaga gccaacttcg ggcggggtac gatgcgcagt
     1861 gcgggataca ggtgtaggag tccctatgga gagtctcaat cgcatctttg aacgcttcta
     1921 tcgcgttgat tcatcccgca ccggttctgg cacaggatta gggctttcga tttcaaagca
     1981 tattatcgaa gcgcacggcg ggaagatttg ggcagaaagt gaagagggac gcggcagtac
     2041 atttttcttt attgttccgt tggacccttc caaggcaaaa gaataacaaa acgacttcca
     2101 gaaccgggga tgctaaaaac gtccatcttg ccatcaaaca actccacgag tttatttaca
     2161 attgcaagcc caagccccgt gccttcatat ttacgcgcta atgtagcatc taattgaaca
     2221 aagggagtga agatgttggc aatatcactt ttcttaatgc caattcctgt atcccaaacg
     2281 acaacctcaa cacagtgttg ttcagatttt tcggtcagcg aaatcccaaa ctctccgcct
     2341 tcgggggtga atttactggc atttcgcagc aaatgaataa gcattttttt catcaatgtc
     2401 acatcggtgt ctacaataat gccttcaggc tgagattgta acgaaacgct ttgcttcttg
     2461 cgcttggcat cggtttctac agaccgtaat gctgcaatac agatatccgt aagagcatat
     2521 gggctcttaa acaaaatttg ttttccgctc tgcaaacgtg aataatcgat caggtcgttc
     2581 acgagagata aaaggcggtg tccatttctc tctataaggt tcactgattt cttttgttta
     2641 tcgctcaacg tatcgtcagt tgaaccttga agggctccgg ctccacccag aatgccagta
     2701 agcggggtgc gcaattcatg actcatggca gccatgaatt catcttttgc ccgcatggct
     2761 ttctccaagt ccgcattgac cacggtcaac tcggctgtac gtgcct
//
LOCUS       DAOQFT010000333         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22053_length_2804_cov_7.448163, whole genome shotgun sequence.
ACCESSION   DAOQFT010000333 DAOQFT010000000
VERSION     DAOQFT010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22053_length_2804_cov_7.448163"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLR93_08875"
     CDS             <1..774
                     /locus_tag="PLR93_08875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNH27120.1"
                     /translation="LNFVESGKSKGTGQPSNISTFKPSNLTPDTGTLGTLIKHTTMRK
                     SIAQHMVESKHTSPHVLTVMEADMSRVAKHRAANKAAFERDGVNLTFTAYFMMAIVAG
                     LKAYPQTNSSWTDEGLLIHKNVNLGMAVSLGEDGLIVPVIKKAEDLSLLAMARAVNDL
                     ANRARTKKLQPDDVKGGTFTLTNHGVSGSLFAFPVINQPQAGILGVGAMQKRVVVIDD
                     AIAIRPMVYMSFVFDHRILDGASADWFLAKVKETLENWA"
     gene            778..1104
                     /locus_tag="PLR93_08880"
     CDS             778..1104
                     /locus_tag="PLR93_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27121.1"
                     /translation="MKTLTGVLEAYYEQGWEGMIVFSFMPNDGNGLNDLITLRDGQLL
                     TIYDQNEATLWSGKIQFVKRGFFDKHKLKADIWSDSKQKGVSYADWMDWFWRQPPLKA
                     TLEFKD"
     gene            1107..1583
                     /locus_tag="PLR93_08885"
     CDS             1107..1583
                     /locus_tag="PLR93_08885"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNH27122.1"
                     /translation="MSDKQKDSPAINKYIHPPIVAMFYIIVALLLGYLVPVLAGMSQT
                     LKNIGLGITFVGFLCGVGAFLEFRKARTTLDPHGSVKTVVTGGIYRFSRNPIYLGFLF
                     MVIGFPLAYGSLWGLVISPFFMVTLSRLVIEKEEAYLEKKFKEQYTSYKSKVRRWL"
     gene            1667..2323
                     /locus_tag="PLR93_08890"
     CDS             1667..2323
                     /locus_tag="PLR93_08890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNH27123.1"
                     /translation="MPIQTSDIQLSVNGKQVYAYLADGKGPGILLLHAWWGLKPFFKQ
                     VCDRIAEQGFTVLAPDLRGGQIAKTIDEAKALMEKSDGQFTGDTVFTAKAHLQTLVKG
                     KLGLIGFSMGAAWSLIAAAYKPEDFGAVVLFYGNEGVDYGKITAKVLGHFCEVDEWEP
                     TEFVDNTFAEFAKAGVDATRHTYPGTAHWFVEEDRPEYDAAAAQLAWSRTYDFLKKTL
                     "
     gene            2440..>2804
                     /locus_tag="PLR93_08895"
     CDS             2440..>2804
                     /locus_tag="PLR93_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH27124.1"
                     /translation="MSENFDVVVIGAGPAGYVAAIRASQLNQKVAIVDKQWMGGVCLN
                     VGCIPSKSLLKNAEVAHTLRHRAKDFGFSFDNLKLDYSVAFKRSRSNSDRLVKGIGFL
                     MKKNNIAVFMGAAQFKAKDT"
BASE COUNT          743 a          649 c          697 g          715 t
ORIGIN      
        1 ttgaatttcg tcgaaagtgg aaagtcaaaa gggacaggtc aaccttccaa catttcaacc
       61 ttcaaacctt caaacctgac acctgacacc ggaacacttg gcactcttat aaagcacacc
      121 accatgcgca aatcgattgc gcagcacatg gtcgagtcca agcatacctc gccgcatgta
      181 ttgacggtga tggaagcgga tatgtctcgg gtggcaaagc atcgtgccgc gaacaaagcc
      241 gctttcgagc gcgacggggt caacctcacc ttcacggcgt atttcatgat ggcaattgtg
      301 gcagggctga aagcctatcc gcagaccaat tcttcgtgga cagatgaagg cttgctcatt
      361 cataagaatg tcaacttggg tatggcggtc tcactgggtg aagatggttt gatcgtgccg
      421 gtcatcaaga aagccgaaga cctgtccctg ctggcgatgg cacgtgctgt caacgatctc
      481 gcaaatcgtg cgcgtaccaa gaaactacaa ccagatgatg tcaaaggcgg cacattcaca
      541 ttgaccaacc acggcgtaag cggatcgctg tttgcattcc cggtcatcaa tcaaccgcaa
      601 gcgggaattc ttggagtagg cgcgatgcaa aaacgagttg tcgtgattga cgatgccatc
      661 gcgatccgac cgatggtgta tatgtccttc gtcttcgacc accgcatctt ggatggcgct
      721 tcagcagatt ggttcctggc gaaggtcaaa gagaccttgg agaattgggc ataacaattg
      781 aaaacgctga ccggagttct tgaagcctat tacgaacaag gctgggaagg tatgattgtc
      841 ttcagtttca tgccaaatga tggaaatgga ctgaatgacc tcatcacctt gcgagacggt
      901 caactactaa ccatctacga tcagaatgag gctacactct ggtctggaaa aatccagttc
      961 gtaaagcgcg gcttttttga caaacacaaa ctcaaagcgg atatctggtc agactcaaaa
     1021 caaaagggcg tctcttatgc cgattggatg gattggttct ggcgtcagcc gccattgaag
     1081 gcaacacttg aatttaagga ttgaaaatga gcgataaaca aaaagacagc ccggcgatca
     1141 ataaatacat ccacccaccc atcgtggcaa tgttttatat catcgttgca ttattactag
     1201 gatatctcgt ccctgtattg gcgggcatgt cacaaacctt aaaaaatatc gggctgggta
     1261 tcacctttgt tggttttctc tgtggagttg gtgcgtttct tgaatttcga aaagcacgca
     1321 ccacgctcga cccacatggt tccgtcaaaa ccgtggtgac gggtggaatt tatcgttttt
     1381 cacgtaaccc catttatctg ggcttccttt ttatggtgat cggctttcca cttgcatatg
     1441 ggtcgttgtg ggggcttgtc atctcaccat ttttcatggt cacactctcg cggcttgtca
     1501 tagagaaaga agaagcctat ctggagaaaa aattcaaaga gcaatacacc agctataagt
     1561 ccaaagtgag gcgttggttg taaatcccaa aggcggcgaa agtcgccttt ttcttttgct
     1621 tgtattttag aacaaatgta cttataatca tttggaggag attgccatgc ctattcaaac
     1681 atctgacatt caattaagtg tgaacggaaa acaagtctat gcctatcttg cggatgggaa
     1741 aggtcccggc attttgttat tacatgcttg gtggggactg aagccattct tcaaacaagt
     1801 ctgtgaccgc attgccgaac aaggctttac tgtgctcgcc cccgatctac gtggcggaca
     1861 aattgcgaaa actattgacg aagcaaaagc cttgatggaa aaaagcgatg gacaatttac
     1921 cggtgatacc gtttttacgg cgaaggcaca tttgcaaacg ctggtgaaag gcaaacttgg
     1981 gttgatcggt ttttcgatgg gcgcggcatg gtcactgatt gcggctgcat acaaaccgga
     2041 ggactttggt gcggtcgtgc ttttctacgg caacgaaggc gtggactatg gcaagatcac
     2101 tgccaaagtg ctgggtcatt tctgtgaggt ggacgaatgg gagcccaccg aatttgtgga
     2161 taacaccttt gcagagttcg ccaaagccgg cgtggatgcg acgcgtcaca cctacccggg
     2221 aactgcccac tggttcgtgg aagaggatcg ccccgaatac gatgccgctg ccgcgcagtt
     2281 agcatggagc cgtacatacg actttttgaa gaagactctc taagaacgac tcaatttgaa
     2341 aattgcttat aaaactccaa cacctcccgg catgggaggt gtttctttag tagtaaaatc
     2401 aagccgtaat ttcactttct taaacgtaat ggagtttcta tgtccgaaaa tttcgatgtt
     2461 gttgtgatcg gtgcgggacc tgccggctat gtagctgcca tccgcgcttc acagttgaac
     2521 cagaaagttg ccattgtcga caagcaatgg atgggcgggg tttgtttgaa tgtaggttgt
     2581 atcccttcaa agtccctttt gaaaaatgct gaagtggcac atactttgcg tcaccgcgcc
     2641 aaggattttg gtttctcgtt cgataacctg aagttggatt acagcgtggc attcaaacgc
     2701 tcacgctcca actcagaccg ccttgtaaaa ggcattggct tcctcatgaa gaagaacaat
     2761 attgctgtct tcatgggcgc ggcacaattc aaagccaaag atac
//
LOCUS       DAOQFT010000334         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22054_length_2804_cov_5.869771, whole genome shotgun sequence.
ACCESSION   DAOQFT010000334 DAOQFT010000000
VERSION     DAOQFT010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22054_length_2804_cov_5.869771"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLR93_08900"
     CDS             <1..684
                     /locus_tag="PLR93_08900"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNH27125.1"
                     /translation="TLPSFSQAIQKGADGVELDAKLTTDGHVIIIHDETVDRTTDGKG
                     KVATFSLEAIRKLDAGSWFDAKFAGTKVPLLEEVFETVGKDKLINIELTNYYTPRDGL
                     VVKVCELIRRHNNAKQIIFSSFLPSNLKIAQQTLPEIPRGLLALPGFMGLWARSFGFM
                     FGDYQASHPHISDANRESIQRAHRVNRRVHVWTANSPEEIQRLRDWGVDGLFTDDPGT
                     AVQALGRVK"
     gene            803..>2804
                     /locus_tag="PLR93_08905"
     CDS             803..>2804
                     /locus_tag="PLR93_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNH27126.1"
                     /translation="MRLIRILLLGIFIFSLVPGTVSAQTPTPPTAVRVILNSMTPEER
                     VGQLFLVTFKGTNTGTDSQIYELITKRHVGGVVLTAGNDNFSETDTLARAHQLIEDLQ
                     QVEWTNSANTAVDPVTGSLERYVYVPLFVGISQEGDGYPTDQILNGLTPLPSEMAIGA
                     TWNTEFAQNVGEVRGQEIAALGFNIFLGPSLDVLETPSSSGGDLGTRVFGGDPFWVGE
                     MGRAYVNGLHTGSNGNMLVIAKHFPGAGGADRLPQEEVATVRKSLEQLKQVEFAPFFA
                     VTGNATQPQETVDGLLVSHIRYQGFQGNIRATTRPISFDQQALGQIMALPELASWYSN
                     GGLLVSDNLGSTAVHEFYAAGGSQFQARIAARDSFIAGNDLLYLGNIKSDDTPDSYTS
                     VIRTIDFFAQKYREDPAFAQRVDASVARILTAKLKLYENFDIGNVIVPAVNLAQSDAG
                     SNITFQVARSSATLISPSQQDLASLLPLPPQPSDRIVFITDSVSVAQCTECAAQPLLA
                     TDALQNVVLRLYGPQSGNQVEEFRLKSYSFDNVSAWLDSPDENQFVADDLDNADWVVI
                     SITDSLRSQPALITRLFRERPTLLLNKQLILFSFGAPYYFDTTTLSRFTAYYALYSKQ
                     PQFVDVAVRLLFQELTPAGASPVSIPAVGYDLIDVTSPSPIQI"
BASE COUNT          669 a          641 c          724 g          770 t
ORIGIN      
        1 actcttccgt ccttttcgca agccatccaa aaaggcgccg acggtgtaga actcgatgcc
       61 aaactcacta ccgacggtca tgtgatcatc attcatgatg agaccgttga ccgcactacc
      121 gatggaaaag ggaaggtcgc tacgttttcc cttgaagcta ttcgaaagtt agatgcgggt
      181 tcgtggtttg atgcaaaatt cgcaggcaca aaagtcccct tgctggaaga agtttttgaa
      241 acggttggga aagataagct catcaatatc gagttgacga actattacac cccgcgagat
      301 gggttggtgg tgaaggtttg tgagttgatc agacgccata acaatgccaa gcagattatc
      361 ttttcctctt tccttccatc caatctaaag attgctcaac aaacccttcc tgaaatccca
      421 cgcggacttc tcgctttgcc tggctttatg gggttgtggg cgcgttcctt tgggtttatg
      481 ttcggcgact atcaagcttc tcatccccat atctcagatg caaaccgtga aagtattcaa
      541 cgggcacatc gtgttaatcg tcgtgtgcat gtatggacgg caaattcccc tgaggaaatt
      601 caacgtctgc gagattgggg cgtagacggt ctctttacag acgaccccgg aacggcggtt
      661 caggcgcttg gacgggtgaa gtgaagcttt ccgaatgccg ggaacaaccc cgggcgcggt
      721 ttgaagcgaa gttccaatgg cgtcgttcaa tgaacggcgt ccattttgtc ttaatagcaa
      781 gttgtgttat aagaatgggg caatgcgctt aattcgtatt cttctcttgg gtatttttat
      841 cttctcgctt gtgccgggga cagtttccgc acaaacgcct acaccgccta ctgcggtgcg
      901 agttatttta aattcgatga cgcctgaaga gcgggttggg cagttatttt tggttacgtt
      961 taaaggtacg aataccggga cagattcaca gatttatgag ctgatcacca agcggcatgt
     1021 gggcggcgtg gtgcttacag ccggaaatga taactttagc gagacagata ctctggcacg
     1081 tgcgcatcag ttgattgaag accttcagca ggtggagtgg accaactcag cgaacaccgc
     1141 tgtagacccg gttaccggct ctcttgaacg atatgtgtat gtgcctttgt ttgttggtat
     1201 ttcacaggaa ggggatggct acccaacaga ccaaattctg aatgggttaa cgcctttgcc
     1261 tagtgaaatg gcaatcggag caacatggaa cacggaattt gcgcaaaatg ttggagaagt
     1321 tcgcggtcag gaaatagctg cgttaggttt caatattttc cttggtccat cgctggatgt
     1381 gttagagacg ccatcttcat cgggcggtga tttgggaacg cgtgtgtttg gcggtgaccc
     1441 gttttgggta ggggagatgg ggcgcgccta tgtcaatggt ttgcataccg ggtcaaatgg
     1501 aaatatgctg gtgatcgcca agcactttcc gggagccggt ggggcagacc gtttaccgca
     1561 agaagaagtt gctacggttc gcaaatctct tgaacaattg aagcaagtag agtttgctcc
     1621 attctttgct gtgacgggga acgcaactca gccacaagaa actgtggatg ggttgttggt
     1681 ttcccatatt cgttatcaag gatttcaagg aaatatccgc gcgaccacac gcccgatcag
     1741 ttttgaccaa caagcgttgg ggcagattat ggcgttgcct gaattggcaa gctggtacag
     1801 caatggtggc ttgttggtga gcgataatct cggaagcacg gcggttcatg agttctatgc
     1861 cgcaggtgga agtcaatttc aagcccgtat tgcagcgcgt gattctttta ttgctggtaa
     1921 tgacttgctt tatcttggca acattaagag cgatgatacg ccagatagtt atacatccgt
     1981 catacggacc attgatttct ttgcgcagaa ataccgtgaa gaccccgcct ttgctcagcg
     2041 tgtagatgct tcagtggccc gtattctgac ggcaaaactt aaactgtatg aaaattttga
     2101 tattggaaat gtcatcgtcc ctgctgtgaa cttggctcaa tcggatgcag gaagcaatat
     2161 tacatttcaa gtggcgcgaa gttctgcaac attgatcagc cctagccaac aagaccttgc
     2221 ttctcttttg cctttaccgc ctcagcccag cgaccgaatt gttttcataa cagactccgt
     2281 cagcgtggca caatgcacag aatgtgcggc acagccactt cttgcgaccg atgcgttgca
     2341 gaatgtcgtt ttgcgtttgt acggtccaca gagcggcaat caggtagaag agttccggct
     2401 taaatcctat tcatttgata atgtaagtgc ctggttggat tctccggatg aaaaccaatt
     2461 tgtggcagac gatttggaca atgccgattg ggtggtaatt tccatcactg actcattgcg
     2521 ttcgcaacca gcactgataa ctcggttgtt ccgcgaacgc ccaactttgc tactgaacaa
     2581 acagttgatt ttgttctcct tcggcgcgcc gtactatttt gataccacta cgctctcgcg
     2641 ttttacggca tattatgcct tgtatagcaa gcaacctcaa tttgttgacg tggcagttcg
     2701 gttgttattc caagaactca cgccagcggg agcgtctcct gtttcaattc ctgcggttgg
     2761 atatgatttg atcgatgtca catcgcccag cccgattcag atca
//
LOCUS       DAOQFT010000335         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22189_length_2793_cov_4.693207, whole genome shotgun sequence.
ACCESSION   DAOQFT010000335 DAOQFT010000000
VERSION     DAOQFT010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22189_length_2793_cov_4.693207"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLR93_08910"
     CDS             complement(<1..676)
                     /locus_tag="PLR93_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH27127.1"
                     /translation="MYILSKDADEYRSILEKSKLPDLIIDKELTETCEILFGSPFSIR
                     DVLGKLPNLRWVQSTWAGVEPLLDPSLRRDYTLTNARGVFGGLMSEYIFGYLLAHERK
                     IFERMEAQKNKQWDTSLTGSLRGKTIGLLGVGSIGAEVAQTAKVFGMTVRGYTRTRKA
                     SAQVDRYYHGDELLEFVNGLDYLVNILPNTKETKKIIDAKTLSALPSHAIFINVGRGS
                     TVDEPAL"
     gene            718..1653
                     /locus_tag="PLR93_08915"
     CDS             718..1653
                     /locus_tag="PLR93_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27128.1"
                     /translation="MNSPKIAFRTFMLQILDYAGMYPPANLSLDEAFKNFVAYQSDPY
                     AWMLSRFVIPAKRLKEISAFNEKISFTTLGRGGKDMDEFLANVNLDIADIRTFCDSHQ
                     AATVDMYEVALPASSLTDKFAANDIVTRAADALNKNGIMVFFEAPFIESGESWEVRAE
                     KLLRALRKVKDKHVGFKLRTGGVTAGAFPSTAQVAWAMAQTREAGVPLKCTAGLHHPI
                     RHFNQSVQTKMHGFLNVFGAGILTAANGISQAEIQAILEDENAENFVFDENGFSWNTL
                     RVGESEIKKARLFATSFGSCSFDEPKEDLQAIKLL"
     gene            1688..>2793
                     /gene="fahA"
                     /locus_tag="PLR93_08920"
     CDS             1688..>2793
                     /gene="fahA"
                     /locus_tag="PLR93_08920"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNH27129.1"
                     /translation="MLKIQVGLNSDFPIQNLPYGIFSTKENPTPRVGTRLGDVVIDLA
                     MLDAENAFGKQYGFFADSSLNRFMAAGRDVWREIRQRLTRLVAEEQASLKSDALIFIH
                     EVRMHLPVEVGDYTDFYASREHATNVGIMFRGKENALMPNWLHLPVGYHGRTSSVVLS
                     GTDVIRPRGQVKPKDAPLDFVASRQLDFELEMGFFVGKGNDLGEPISIEDAHEHIFGM
                     VLLNDWSARDIQAWEYQPLGPFLAKNFATSISPWVVTMDALEPFRVKGPLQDPTPLPY
                     LGSNANGYDIQLEVSLQSARMDAPQVISHSNMKHLYWSIDQMLAHHTITGCNMRVGDL
                     CGTGTISGPTEDSYGSLLELTWRGERPIQLSSGE"
BASE COUNT          729 a          593 c          669 g          802 t
ORIGIN      
        1 agagcgcagg ttcatctact gttgagccac gccccacgtt tatgaatatg gcatgcgaag
       61 gtaaagcgct taaggttttt gcatctataa tctttttggt ttctttcgta ttcggaagaa
      121 tattgacgag ataatccaat ccgttcacaa attcaagcaa ctcatcccca tgataatacc
      181 tatctacttg tgcgcttgct tttctagttc gtgtatatcc gcgcacagtc atgccaaaga
      241 cttttgcagt ttgcgctact tctgcgccaa ttgagccaac tcctaataac ccaatcgttt
      301 ttccacgcaa cgaaccagtg agggatgtgt cccattgctt gtttttttgt gcttccattc
      361 gctcaaagat cttccgctcg tgtgctagaa gatatccaaa aatgtattca gacattaatc
      421 cgccaaacac acctctcgca ttggttaatg tgtaatccct ccgaagagat ggatctaaca
      481 gaggctctac acctgcccat gttgattgca cccaacgcaa gtttggaagt ttccccaaca
      541 catcacgaat agagaatgga gagccaaaaa gaatctcgca ggtttcagta agctctttat
      601 cgattattaa gtcaggtagc tttgactttt ctaatattga tcgatattca tctgcatctt
      661 tactaagaat atacatgtgt ggcatagtgc cccaatttta cccgctataa tacagatatg
      721 aattctccga aaatagcttt ccgtacattc atgttacaaa ttttggatta tgctggaatg
      781 tacccccctg caaatttgtc gctagatgaa gctttcaaga attttgttgc atatcaatcc
      841 gatccttatg catggatgct gtcacggttc gtgattcccg ccaaacgctt gaaggagatt
      901 tcagccttca atgaaaaaat atcgtttaca actctagggc gtggaggcaa ggatatggac
      961 gaatttcttg ccaatgtgaa cttggatatt gcagacatcc gcacattttg tgactctcac
     1021 caagccgcta cggtagacat gtacgaagta gccttgcccg cttcttcttt gaccgataaa
     1081 tttgcagcca acgatattgt tactcgcgcc gccgatgcct taaacaagaa cggcatcatg
     1141 gtcttttttg aggctccttt tattgagagt ggagaaagct gggaagtacg agcggagaaa
     1201 ttgctacgtg ccttgcgcaa agtaaaagat aaacatgttg gctttaaact tcgcaccggc
     1261 ggtgtgacag cgggcgcatt tccatctaca gcgcaagttg catgggcgat ggcgcaaacc
     1321 cgtgaggcgg gtgttccgct caagtgtaca gctgggctgc atcatcctat acgacatttc
     1381 aatcaaagtg tgcaaaccaa aatgcatggc tttctgaatg ttttcggggc agggatattg
     1441 acggctgcga acggaatctc acaggcagag attcaagcta ttttggaaga tgaaaacgct
     1501 gaaaactttg tctttgatga aaatggattt tcttggaata ctttgcgtgt aggcgaatca
     1561 gagataaaga aggcaagatt gtttgccacg tcttttggta gttgcagttt cgatgagcct
     1621 aaagaagatt tgcaagctat aaaacttttg tagtttcagt taacaacctc atttataagg
     1681 tgaaactatg ctgaaaattc aagttggtct aaattctgat ttcccaattc aaaatctacc
     1741 ttatgggata ttctctacaa aagagaatcc taccccacgt gtggggacac ggttaggtga
     1801 tgttgtcatt gatcttgcca tgttggatgc tgaaaatgcc tttggtaagc aatatggctt
     1861 ctttgcagat tcatcgctca accgcttcat ggcggcagga cgagatgtgt ggcgggaaat
     1921 tcgtcagcgt ttgactaggc tagtggcgga agagcaggca tcccttaaga gtgatgcgtt
     1981 gattttcatc cacgaagtac gtatgcattt acccgttgag gttggtgatt acactgattt
     2041 ttacgcatct cgcgaacatg ctacgaatgt ggggattatg ttccgtggaa aagaaaatgc
     2101 gctcatgccg aattggttgc atttaccagt tggataccat gggcgcacta gttcggtggt
     2161 gctttcgggt acggatgtga ttcgtccgcg tggacaggtg aaaccgaaag atgcgccgtt
     2221 ggatttcgtt gcatcgcgtc agttggattt tgaattggag atgggctttt ttgtggggaa
     2281 gggaaatgat cttggtgagc ctatttcaat tgaagatgct catgagcata tctttggcat
     2341 ggtgttgctc aatgactgga gcgcgcgtga tattcaggcg tgggaatatc agccgttagg
     2401 accgttcctt gccaaaaatt ttgctacatc catttcgccg tgggttgtga caatggatgc
     2461 tttggaacca ttccgcgtga aaggaccact gcaagaccca accccgttgc cgtatcttgg
     2521 ctcgaatgca aacggatacg atattcagtt ggaggtgtct ttgcaatcag ctcgaatgga
     2581 tgcgccgcaa gtcatcagtc attcgaatat gaagcatttg tattggagca tcgaccagat
     2641 gcttgcacat cacaccatca cggggtgcaa tatgcgtgtg ggcgatttat gcggtaccgg
     2701 cacgattagc ggaccgacag aagatagcta cggctcactg cttgagttaa cttggcgcgg
     2761 cgagagaccg attcagttat caagcggaga aga
//
LOCUS       DAOQFT010000336         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22306_length_2785_cov_5.064103, whole genome shotgun sequence.
ACCESSION   DAOQFT010000336 DAOQFT010000000
VERSION     DAOQFT010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22306_length_2785_cov_5.064103"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLR93_08925"
     CDS             <1..487
                     /locus_tag="PLR93_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27130.1"
                     /translation="DNELLSFYDQLGLFRSPLITLPVAESQPTEKVQDLRASPLQVAL
                     ATAALSNHGVIPAPRIATAVNTPEQGWVVLPVLDKPRELFSAEIADEAAHAFVQQGNA
                     YWKYIGQGVINKTTVTWLIAGTPPDWQGAPLAVVIAVEENNPQLVEQIGASLFNSVLS
                     Q"
     gene            complement(533..946)
                     /locus_tag="PLR93_08930"
     CDS             complement(533..946)
                     /locus_tag="PLR93_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNH27131.1"
                     /translation="MTHIITSLCVRDRGCIEVCPVECIVPGNPADGFPMVYIDPDTCI
                     DCGACVPECPFSAIFPEDEVPSSYTAKGTEYINRVGLSGHYEASNHHGKPVVLETTKK
                     LDAGETVDLTPDTQLNYDFFKSGPGYSAKDTDSGS"
     gene            complement(1067..1669)
                     /locus_tag="PLR93_08935"
     CDS             complement(1067..1669)
                     /locus_tag="PLR93_08935"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNH27132.1"
                     /translation="MAFELPKLAYAYDALEPHIDARTMEIHHTKHHQTYITNLNGAVE
                     KTPELQGKSLEALLSDLNAVPEGVRAVIRNHGGGTYNHNLFWEIMGPNAGGAPKGDLA
                     KAIDASFNSFDAFKDEFTKAATTRFGSGWAWLVKKGSGLAVVSTANQDTPLSDGLTPI
                     LGIDVWEHAYYLNYQNRRPDYITAFYNVINWEAVSAKFAA"
     gene            complement(1825..2064)
                     /locus_tag="PLR93_08940"
     CDS             complement(1825..2064)
                     /locus_tag="PLR93_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27133.1"
                     /translation="MSTVKSEIAKLVVKKVKGITEPQAEQAVAVVLEFVEKKLPPHIG
                     SKLDAILAGNVDLSDGIGLDDAAGLLGGLLGGDKK"
     gene            2210..>2785
                     /locus_tag="PLR93_08945"
     CDS             2210..>2785
                     /locus_tag="PLR93_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNH27134.1"
                     /translation="MDSKTRFLLPIALIVATVAVSTSSIFIRFAQAGGAPSLVIAALR
                     VTFATLMIAPVALTRHMDEIKRFTRIEFLLGVASGIFLAAHFATWITSLEYTSVASSV
                     VFVGTGPLWVALLSPMLLKEHISRTTIVGLGLAVIGGIIIGLADACTWNNGLSCPALN
                     DILKGRAMWGNFLALAGAWAVTGYLIIGRKLR"
BASE COUNT          660 a          647 c          760 g          718 t
ORIGIN      
        1 cgataacgag ttgctctctt tctacgatca actcgggctt ttccgttccc cattaattac
       61 cttacccgta gcagaaagcc agcctactga aaaagtgcaa gacctgcgag ccagcccatt
      121 acaagttgca ttggcaacag cagcattgag caaccacgga gtgatccctg cgccacgcat
      181 tgcaacggcg gtaaacacgc ccgaacaggg atgggtggtg ctgccagtgt tggataagcc
      241 gcgagagttg ttctcagccg aaattgcgga tgaagcggca catgcttttg tccagcaagg
      301 gaatgcgtat tggaaatata taggtcaggg tgtgatcaac aagacgacgg tcacatggct
      361 gatcgctgga acgccgcccg attggcaagg tgctccgttg gcagtggtga tcgcggtgga
      421 agaaaataat ccacaactcg ttgaacagat cggcgcaagt ctattcaatt cagtgttgag
      481 tcagtagaat caaaaaagcc aggcaggtgc ctggcttttt attttcaatg gactagcttc
      541 cgctatcggt gtctttggcg ctgtagccag gaccactctt gaagaagtca tagttgagct
      601 gtgtatcagg agtgagatcg acggtttcac cagcatcgag cttcttggtg gtttcgagaa
      661 caaccggctt gccgtggtga ttggaagctt cgtagtgacc agagagacca acacgattga
      721 tgtactctgt gcctttggca gtataagatg aaggaacttc atcttcaggg aagatggcac
      781 tgaaaggaca ttcgggcacg caagcgccgc agtcgatgca ggtgtcagga tcgatataga
      841 ccatggggaa gccatcagca ggattgccgg ggacgataca ttcgacgggg caaacttcga
      901 tgcatccgcg gtcacgaacg cacaaactgg taataatatg agtcatttct tttctccttg
      961 tgggatatag acaaacgatt tcttcctatt ataactttgt ttacgtgagt gacaaggaca
     1021 aaaaaacacc ccgtattgct acggggtgtt ttttacatcg tacgtactaa gcagcaaatt
     1081 ttgcagagac agcttcccaa ttgattacat tgtaaaacgc ggtgatgtaa tcggggcggc
     1141 ggttttgata gttcaagtag taagcgtgtt cccacacgtc gataccgaga atgggagtga
     1201 gaccatcaga aagcggagtg tcttgattgg ctgtggagac aacggcgagt ccgctgccct
     1261 tcttgacgag ccaagcccag ccggagccga agcgagtggt ggcggctttg gtgaattcat
     1321 ccttgaatgc atcaaaggag ttaaaggaag catcaatcgc cttggcgagg tcacctttgg
     1381 gggcgccgcc ggcgttgggt cccataattt cccagaaaag gttgtgattg taggtgccgc
     1441 cgccatgatt gcggataacg gcgcgaacgc cttcgggcac agcgttcaaa tcgctgagca
     1501 aggcttcgag ggatttgccc tgaagttcgg gggtcttttc aacagcgcca ttgaggttgg
     1561 tgatataggt ctgatggtgc ttggtgtgat ggatctccat cgtgcgcgca tcaatgtggg
     1621 gttcaagcgc atcgtacgcg tatgcaagtt tgggaagttc aaaagccata atagttctcc
     1681 ttcgagttga ttatttgagt tggtctttcc attgtatccc gcccgtgggc tgggcgcaag
     1741 ataaaataaa ttttattaca aatatctaat aaatctaaag gcaaaaaaag ccgccgtaat
     1801 ttcttacgac ggcatttcta caaattactt cttatcgcca ccaagcaagc cgccgagcag
     1861 acctgcggcg tcgtcgagac cgatgccatc gcttaggtca acgtttccgg cgagaatggc
     1921 atcaagcttg ctgccaatat gcgggggtaa tttcttttcg acgaattcaa gcacaacggc
     1981 aactgcctgt tcggcttgtg gttcggtgat gcctttcact tttttgacga ccaatttggc
     2041 aatttcagac ttaactgtgg acattatggt ttctcctttg ttcgtaataa cttgaatgta
     2101 gcacacctta gattgaagtg caagattaga ggtcaattac caagataaat gcaaacgttt
     2161 tttgtatctc gcggtagaac agatgaatta aggtaaactc gggcacatta tggattcaaa
     2221 aacccgcttc ctcctcccga ttgctttgat cgttgctacc gtggctgttt ctacctccag
     2281 catttttatt cgctttgcac aggcaggcgg agcgccttcg ttggtgatcg ccgccttgcg
     2341 cgtgaccttt gccactttga tgatcgcccc ggttgccctg acccgccata tggatgagat
     2401 caaacgcttc acgcggatcg agtttctgct tggcgtggct tcgggcattt tcctcgccgc
     2461 acactttgca acatggatca cttcgctgga atacaccagt gttgccagct cggtggtgtt
     2521 cgtagggacg ggtccgctgt gggtggcgct gctttcgccg atgctgttga aagagcatat
     2581 ctcgcggact acgatcgtcg gcttggggtt ggctgtgatc ggcgggatca tcattgggct
     2641 ggcagatgcc tgcacatgga ataatggtct ctcctgcccg gcgttgaacg atatcttgaa
     2701 aggacgcgcc atgtggggca acttccttgc cttggctggc gcatgggcag tgacggggta
     2761 tctcatcatc ggcagaaaat tgcga
//
LOCUS       DAOQFT010000337         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22487_length_2772_cov_7.703349, whole genome shotgun sequence.
ACCESSION   DAOQFT010000337 DAOQFT010000000
VERSION     DAOQFT010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22487_length_2772_cov_7.703349"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1619
                     /locus_tag="PLR93_08950"
     CDS             174..1619
                     /locus_tag="PLR93_08950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNH27135.1"
                     /translation="MTQPTYTDFYTLIPYLILTVWACVLLIVDLFIPKGRKGITAFLA
                     ALGIAISLGYSITQTGIESTGFSSMVSLDGFSTFANILLLGSGLFAIALAYGYTKRMG
                     IERGEYYTLLLFSLTGMMLMAQANDLIMVFIALELLSIPLYVLSAISRKLTSEESGVK
                     YFLLGAFASGFVVYGTALIFGATGSTNLNVILVQAASGVTSGLLLTIGAALLLVGFGF
                     KVAAVPFHMWTPDVYQGAPTSVTAFMAAGAKIAGFVALFRVFSTAFPALSSDLTNILW
                     ALSALTMIVGNLTAISQTNIKRMLAYSAIAHAGYILMAFVPYGNAKVAPTAIAAGLFY
                     LAAYALSNFGSWGVVIALEKAEGKGLEIEDYAGLAKKYPALAVAMTVFMLSLIGFPPT
                     LGMVGKFYLFRSALAGGFPGLALIGVVTSLISAYYYLRVVVNMYMKEGDPEVEREPWL
                     GLTTALTAVVTVAVSLIPQFLFTLAYTAVMR"
     gene            complement(1664..2386)
                     /locus_tag="PLR93_08955"
     CDS             complement(1664..2386)
                     /locus_tag="PLR93_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27136.1"
                     /translation="MQIAIIFFGVLILLVLIWLLLTELRREKSEEQSEPVSKNSQRVF
                     TKWLENFKTWSGERYPINIERLGFTVLWSIFFLSLLDHLQTRSGVTGTLIYFLVILPH
                     EAGHFICSPFGWFLSIAGGSIWQVLLFVLFGFIVLFRKRRNEALGWWAVAGHSLISLS
                     TYIADASARSLPLIPIPDPSRHDWGNMLEYLGLLQYDWLFAKISIITGGAIVLSCVVA
                     GLFFTWYQPQNLFNKPEIDTAK"
     gene            2501..2575
                     /locus_tag="PLR93_08960"
     tRNA            2501..2575
                     /locus_tag="PLR93_08960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2533..2535,aa:Lys,seq:ctt)
     gene            complement(2611..2757)
                     /locus_tag="PLR93_08965"
     CDS             complement(2611..2757)
                     /locus_tag="PLR93_08965"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HNH27137.1"
                     /translation="MNEMTATGGVLLMGVGISNLLELKKIRVGNFLPALVVAPLIVWV
                     LGKL"
BASE COUNT          675 a          729 c          624 g          744 t
ORIGIN      
        1 gatatcctcg cccctgcggt tgagaagttg ctgttaccga taacggcctt gttacattaa
       61 taatattttt gtcactgcgc gggtgaaacc cgaaacagac tcagacgtaa ccatgagatt
      121 ttgtttcttc agaaagaata cctgccttgc aatgacaaag acatggagcg ttcatgacgc
      181 aaccaaccta caccgacttt tacacactca tcccttatct catcctgacc gtttgggcat
      241 gtgtgttgct gattgttgac ctattcattc caaaaggtcg caaaggcatc actgcatttc
      301 ttgctgcatt gggcatcgcc atctcgcttg ggtattcaat tacgcaaaca ggtattgaaa
      361 gcaccggctt cagcagcatg gtttcgcttg acggcttctc cactttcgcc aatatccttt
      421 tgctcggaag tggactcttc gccatcgccc ttgcctacgg ctataccaaa cgcatgggca
      481 tcgaacgcgg cgagtactat actctgctgt tattcagcct caccggcatg atgctcatgg
      541 cacaagcgaa cgacctcatc atggtcttca ttgcgctcga attgctttcc atcccgctct
      601 atgtcctctc tgcgatctcc cgcaagctga cctctgaaga atctggcgtg aaatacttct
      661 tgctcggcgc cttcgctagc ggatttgtgg tctatggaac cgctctgatc ttcggtgcga
      721 cgggctctac caacctcaat gtgatcttgg ttcaagccgc ctctggcgtg acctcgggct
      781 tgctcctcac cattggggct gcgcttttgc tggtaggctt tggcttcaaa gtagccgcag
      841 ttcccttcca catgtggacc cccgatgtct accaaggtgc accaacttct gtcacagctt
      901 tcatggcagc tggtgcgaag attgctggtt ttgtagcatt gttccgcgtt ttttctactg
      961 cgttccctgc tctctcttca gacctcacca atatcctctg ggcgctctct gcgttgacca
     1021 tgatcgttgg taacctcacg gcaatttcac agaccaatat caaacgcatg ttggcatact
     1081 ctgccattgc gcatgcaggg tatatcctca tggcgttcgt cccttatggg aatgcgaaag
     1141 tcgctccgac tgcgattgcg gctggcttgt tctatcttgc ggcatacgcc ctcagcaact
     1201 tcggttcttg gggtgtggtg attgctcttg aaaaagccga aggcaaaggg ctcgaaattg
     1261 aagattatgc tggcttggca aagaaatatc ccgctctcgc agtcgctatg actgtcttca
     1321 tgctctccct gatcggcttc cctccaaccc ttggtatggt gggtaagttc tacctcttcc
     1381 gctctgccct cgcaggtggt ttccccggtc ttgcccttat cggcgtggtg acctctctta
     1441 tttccgcata ctactacttg cgcgttgtgg tcaacatgta catgaaggaa ggcgaccctg
     1501 aggttgaacg tgaaccttgg cttggcttaa ccacagccct tacagcagtc gtaactgtag
     1561 cagtgagcct cataccgcaa ttccttttca cactggcata tacagctgtg atgcgataaa
     1621 cataaaaaag acaaaaagag cgctgacatc tgtcagcgct cttttatttt gcagtatcga
     1681 tttctggctt gttgaataag ttttgaggct gataccaagt aaagaaaagc cctgctacaa
     1741 cacaagagag aacaattgca ccaccggtga taatcgaaat cttcgcaaat aaccagtcat
     1801 attgtagcaa ccccaaatat tcgagcatat tgccccaatc atgccgtgac gggtctggga
     1861 tggggatgag gggcaggctg cgggcactgg catctgcaat gtaagtggaa aggctgatca
     1921 ggctatgacc tgcaactgcc caccaaccca aagcttcatt gcgccgtttg cgaaagagga
     1981 cgatgaagcc aaagagtacg aataaaagca cctgccagat tgatccgcct gcaatgctta
     2041 gaaaccaccc gaaaggggag cagatgaaat gcccagcttc gtgaggcaaa attaccagaa
     2101 aatagatcaa cgttccggtc acgcctgagc gagtttgcag atgatccaat aaagaaagga
     2161 agaaaatgct ccagagtacg gtaaacccaa gtcgctctat gttgatgggg taacgttctc
     2221 cagaccatgt tttgaagttt tcaagccact ttgtaaagac tctctggctg tttttagaaa
     2281 caggttcact ttgctcttcc gatttttctc ttcgtaattc ggttaaaagc agccaaatta
     2341 gaaccagaag gataagaacg ccgaaaaaaa tgatcgcaat ttgcatggaa gccttcttga
     2401 ttataaaagc tattttccgg cggctctcct tgcaaaaaac gtacgagttg taattattga
     2461 cacaataccc ctttgcggta taataccgcc cgcacgaaat ggctccgtag ctcaatggca
     2521 gagcagttga ctcttaatca attggttgag ggttcaaatc cccccggagt caccagtcaa
     2581 aaaacatccc catttgggga tgttttttgt ttatagcttt cctaacaccc acacaatgag
     2641 tggagcaacg accagcgccg gtagaaaatt cccaacccga atctttttga gttccagtag
     2701 attactgatt cccaccccca tcaacaatac cccacctgtg gcggtcatct cattcatcat
     2761 ggggtcactg at
//
LOCUS       DAOQFT010000338         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22488_length_2772_cov_5.964667, whole genome shotgun sequence.
ACCESSION   DAOQFT010000338 DAOQFT010000000
VERSION     DAOQFT010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22488_length_2772_cov_5.964667"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLR93_08970"
     CDS             complement(<1..776)
                     /locus_tag="PLR93_08970"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNH27138.1"
                     /translation="MHTILKSEKKEVTIGFDKPFVIIGEKLNPTGIKKLGQALVERNM
                     DYVKHLAKRQVDWGADVLDVNVGHPQIEEAEVMPLVVEAVQSVTDVPLCIDSNEPKIL
                     EAGLNAIKGGKPLVNSVNGEEKQLSTVLPIVKARGAAVIGLTIADEGIPPTAEGRLAA
                     AAKIIERAAKVGIPVEDIIIDPLVMTVGHNSLAATVTLKAIELIKKEFDVNISLGASN
                     VSFGLPDRHSVNAAMLSIAMLTGVTTSITDPIKLGSTIRAT"
     gene            complement(822..1979)
                     /locus_tag="PLR93_08975"
     CDS             complement(822..1979)
                     /locus_tag="PLR93_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNH27139.1"
                     /translation="MIKQAHAISEDLIEWRRDFHMHPEIGFDLHRTAGIVADELEKMG
                     YRVKRGVGKTGVVAEIGEGGKLVAIRADMDALPIFEQNETDYISQNQGKMHACGHDSH
                     TAMALGAAKILSQEKLNGRVRFLFQPCEETADEEGKSGAQRMYAEGATEGVDFVIAQH
                     VDPSHPVGTITINEGPSGGGVTSWSATIYGKGGHGAEPHVSNDPFVMLAHVITGINSV
                     VSRRLDPFEPAVISIGAVNGGFAENVIPDKIEMIGTIRFTSLDIEKKIREELTKVFEI
                     VKVLGGDYKLNFLFGGMPIINDKFVSDTIASVGKELLGEGSVLPMDKTLGAEDFPEFL
                     RDAPGAMYMLGVRIEDRPLYELHHPKFDLDERALPIGTAILAETAKRFLNS"
     gene            complement(2067..>2772)
                     /locus_tag="PLR93_08980"
     CDS             complement(2067..>2772)
                     /locus_tag="PLR93_08980"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNH27140.1"
                     /translation="QQNAETLAPLVLAQILNPGCGVVYCGRLGLLEPRTGLIWAGVEL
                     GMSSAATVQLGHYYGFAVNVYGFSTNAHTLDAQNAFERGLNASLPALAGADELSGIGE
                     MEAGVMGSFAQMVLDNELAKSVHRQRQGLSADAEHLAVEVIGNVMDSNRNYLASKHTL
                     KHLRAGEMALTKLAERNSWDTWEDKLGRKQMADSATVEAERILREHQVQPLDPAQEAE
                     LDKILAAAQRETVKKK"
BASE COUNT          623 a          747 c          678 g          724 t
ORIGIN      
        1 gtagcacgga tggtgctgcc aagtttgatt gggtctgtga tggacgtggt gacaccagta
       61 agcatggcaa ttgaaagcat ggcagcgttg acggagtgcc tatctggcag cccaaatgac
      121 acattgctcg caccgaggct gatgttcaca tcaaattctt ttttgatcaa ctcaatagct
      181 ttgagagtta cggtcgctgc caggctattg tgcccaaccg tcataaccag cggatcgatg
      241 atgatgtctt caacgggaat gccgaccttt gccgcgcgtt cgatgatctt cgccgctgca
      301 gcgagacgcc cttctgcggt aggcgggatg ccttcatcgg caatcgtcaa gccaatgact
      361 gctgcgcctc ttgccttgac gatgggcagc actgtgctaa gttgcttttc ttccccgttg
      421 acagagttca cgagcggctt gccgcctttg atggcattca aacccgcttc gagaattttc
      481 ggctcgttcg agtcgatgca gagcggcaca tcggtcacag actgaaccgc ttcgaccacc
      541 aacggcatga cttccgcttc ttcaatctgc ggatgcccca catttacatc cagcacatcc
      601 gcgccccaat caacctggcg tttggcgagg tgcttgacat aatccatgtt gcgctcaacc
      661 aaggcttgcc ccagcttttt gatgccggtg gggttgagtt tttcaccaat gatgacgaat
      721 ggcttgtcga aaccgatcgt gacttctttt ttctctgact tgagaatggt atgcacaggt
      781 gccctccgaa agtttaatta tgagacgata gagtctctag attatgagtt caaaaagcgt
      841 tttgctgttt cagccaaaat ggctgtacca atgggcaagg cgcgttcatc gaggtcgaac
      901 ttggggtgat gtaattcata caaaggacga tcttcgatac gcacacccag catgtacatg
      961 gcgccgggag catcacgtag aaattctgga aagtcttccg cacccaaggt cttatccata
     1021 ggcaagacac tgccttctcc tagcagttct ttgccaacgc ttgcaatcgt atcggacaca
     1081 aatttatcgt tgatgattgg catgccgcca aagaggaagt ttaatttgta atcaccgcca
     1141 agcactttga caatctcgaa caccttggtc aactcttcgc gaatcttttt ctcaatatcc
     1201 agcgaagtga agcggatggt tccgatcatt tcgatcttat cggggatgac attttctgcg
     1261 aacccgccat tgaccgcacc aatgctaatg actgctggct caaacgggtc aagcctacga
     1321 gagaccacgg aattgattcc cgtgatcacg tgcgccagca tcacgaacgg atcgttggaa
     1381 acatgcggct cggcaccgtg cccgcccttg ccatagatcg tcgcagacca ggatgtaacc
     1441 ccgcctccgc tcggaccttc attaattgta atggtcccaa ctgggtggct tgggtctaca
     1501 tgctgcgcaa tgacaaagtc cacgccttcg gttgcacctt ctgcgtacat gcgttgtgcg
     1561 ccgcttttgc cttcttcatc ggcggtctct tcgcaaggtt ggaagagaaa acgaacacgt
     1621 ccgttcagct tttcttgaga aaggattttt gccgcaccca atgccatggc agtatgcgag
     1681 tcatgaccac aggcgtgcat tttgccttgg ttttgtgaga tgtaatcggt ctcattctgt
     1741 tcaaagatcg ggagagcatc catatcagca cgaatggcaa ccaacttgcc accttccccg
     1801 atctctgcta caacaccagt cttgccaacc ccacgtttta cacgatagcc catcttttcc
     1861 agttcatctg ccacaattcc ggcggtgcgg tgcaggtcaa aaccgatttc cgggtgcatg
     1921 tgaaaatcgc gccgccattc gatgaggtct tctgaaattg catgtgcttg tttgatcatt
     1981 ctatattctc ctgtatttaa gtatacaagc aaacttgagc acaaggatac cttctattcc
     2041 ttgcgccaat taacttatat acctgcttac tttttcttta cggtctcacg ttgtgccgcg
     2101 gcaaggattt tatccaactc agcttcttgg gctgggtcga gcggttggac ctgatgttcg
     2161 cgcaaaattc tttcggcttc aacggtggca gagtctgcca tttgtttgcg ccccagtttg
     2221 tcttcccagg tatcccacga attacgttcg gcaagtttgg tcaatgccat ttcgccggcg
     2281 cgcaggtgtt tgagtgtatg cttgctggca aggtagttgc ggttggaatc cattacgtta
     2341 ccaatcactt ctaccgccaa atgttcggca tctgccgata agccttgacg ttgacgatga
     2401 acactctttg ccaattcatt atcgagcacc atttgcgcga aggaccccat cacaccggct
     2461 tccatttcgc caatgcccga gagttcatcg gctccggcta gggcggggag agaggcgtta
     2521 agtccgcgtt cgaaagcgtt ttgagcgtcg agcgtatggg cattggttga aaatccatac
     2581 acattgacgg cgaacccgta ataatgcccg agttgaaccg tagcggcaga agacatgccc
     2641 agctccacac ctgcccaaat caagcctgtg cgcggctcca gcagcccaag gcgcccgcaa
     2701 tagaccacac cgcagcctgg gttcaaaatc tgtgcgagaa ccaacggagc cagcgtttct
     2761 gcattttgtt gg
//
LOCUS       DAOQFT010000339         2771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22505_length_2771_cov_5.915317, whole genome shotgun sequence.
ACCESSION   DAOQFT010000339 DAOQFT010000000
VERSION     DAOQFT010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22505_length_2771_cov_5.915317"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLR93_08985"
     CDS             <1..612
                     /locus_tag="PLR93_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27141.1"
                     /translation="NEFAWWMLGTGILLGLGILLMSVSRRAAYAVILSAALVIPAYWS
                     VMTVTNGSNNNLPTAYEGQNRNGQDGFLASPQQQNQGNRGNDRSVNTELLAYLEANTV
                     DMKYLMAVPSSQQGSQYVIETGRPVLYMGGFSGGDAVVTVDDLKELVANGELRYILYG
                     GDRGNQEITAWLNSSCAIVNDFNNLSQAQQGPQNQAMTLYSCR"
     gene            complement(644..973)
                     /locus_tag="PLR93_08990"
     CDS             complement(644..973)
                     /locus_tag="PLR93_08990"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNH27142.1"
                     /translation="MSALSIWLLFVAIGAGTFTLRFLFIYLFGKVEMPTWLSRALRFV
                     PAAALAALVFPALTHPAGHLDISLTNFRLLAGLGGALVAWRTRNVLLTILVGMALLWS
                     LQAAFGM"
     gene            complement(970..1671)
                     /locus_tag="PLR93_08995"
     CDS             complement(970..1671)
                     /locus_tag="PLR93_08995"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNH27143.1"
                     /translation="MKWNKDFIAGIKGAAPILIGVIPFAMITGVGAVSVGLSFWESMG
                     MSVFVFAGASQLVVFQLMSVGTPWIIMVLTAWGVNVRFTMYSATLAPYLQKLSTGRKA
                     LLAYMLSDQAFGVSMSRFISTDKEIDYRWYYFGAALIIWLAWQVSAVIGTLLGALVPA
                     SLGFDFAFPLSFMALMFGALRDRPTVVAALTGGFIAVLAKSLPYNTGLVLATFLGIIA
                     GYVAESLKLKEEAVL"
     gene            1845..2753
                     /locus_tag="PLR93_09000"
     CDS             1845..2753
                     /locus_tag="PLR93_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27144.1"
                     /translation="MKRTIALTLSLLWLTACVTLTNPAPLIPTETVPTTPSVSAADNT
                     PTYVPLINNSALLYISGTTHIESKPQSWPNIDAFLNFLQNITAQGLKWSVGADIGWLE
                     GEPRAAELIQASEALGVQWDIHAHNPQERAKAAYLITQLGGHPTSVISGMLVSEFSSI
                     GPQTYQGFTWTPHVLWGGTNCPGHRPGCDDLAAGIWIPLSAEQYTTHDPNGTLIRVGG
                     GSHQLADGLVLAESIANRQYPYPIISFTLMVSPTSLTIVNSNDSLEQITAFANDMKQY
                     PFVYWAYIEETAQAWTKAGKVPSRIE"
BASE COUNT          816 a          731 c          581 g          643 t
ORIGIN      
        1 aacgaatttg catggtggat gcttggaacg ggcatcctgc ttgggttggg aatcctgctc
       61 atgtccgttt cgaggcgtgc ggcgtatgcc gtcatcctca gtgcggcttt ggtcatcccc
      121 gcttattgga gtgttatgac cgtcaccaat gggtcgaaca acaacctccc cactgcctat
      181 gaagggcaga accgcaacgg gcaagatgga ttccttgcca gcccgcaaca gcaaaatcaa
      241 ggcaaccgcg gtaatgaccg cagtgtgaat acggaactgt tggcatactt ggaagcaaat
      301 acggttgaca tgaagtatct tatggcagtt ccatcttcac aacagggttc acaatatgta
      361 atcgaaaccg ggcgcccggt cttatacatg ggtggtttca gcggcggtga tgccgttgta
      421 acagtggatg atctgaaaga attagttgcc aatggagagt tgcgttacat tctctacggc
      481 ggcgatagag gcaaccaaga aattaccgct tggctaaata gctcatgtgc gattgtgaat
      541 gatttcaata atctatctca agcgcagcag ggtccacaaa atcaagccat gaccttatac
      601 tcttgcagat aataaaaagc cccgagataa tctcggggct tttttacatc ccaaatgctg
      661 cttgaagaga ccacaacaaa gccatgccca ccaaaatggt aagcagaaca tttcttgtgc
      721 gccacgccac caatgcccca ccaagccctg ccaagagacg gaaattcgtg agcgaaatat
      781 caaggtgccc cgcagggtga gtcaacgctg ggaagaccaa cgccgccaac gctgccgcag
      841 ggacgaacct taacgcccgg ctcaaccaag tgggcatctc aactttgcca aagagataaa
      901 taaacaaaaa ccgaagcgta aatgtgcccg ctccaatcgc cacaaagaga agccaaatcg
      961 ataatgcgct cataggactg cctcctcttt tagcttcaga ctttcagcaa catacccggc
     1021 aataattcca agaaaggttg ccaataccaa cccagtgtta tacggcaagc ttttcgccaa
     1081 cacggcgata aaaccgccgg tcaatgctgc tactactgta gggcggtctc gcaaagctcc
     1141 gaacatcaac gccatgaaac tgagcggaaa ggcaaagtca aagccgaggc tggcgggtac
     1201 aagagcacct aacaatgtcc caatcacagc gctaacttgc catgccaacc agatgatcag
     1261 cgcagcgcca aagtaatacc accgatagtc tatttcttta tcggtggaaa taaagcggct
     1321 catactgacc ccaaatgcct ggtctgagag catatatgcc aacaatgctt tacgtcccgt
     1381 agaaagtttt tgtaaatatg gcgccaatgt agcactgtac atcgtaaaac gtacatttac
     1441 cccccacgcc gttaaaacca taataatcca tggggtgcct acactcatca gttgaaacac
     1501 caccaactga gaagcccccg caaacacaaa cacagacatc cccatcgact cccaaaatga
     1561 cagaccaaca ctcaccgccc ccaccccagt gatcattgca aaaggaatca ctccaatcaa
     1621 gattggggct gcccctttta ttccagctat aaaatcttta ttccatttca taattcatcc
     1681 atattgtatt ttttaggggg caattctacc cctgaactct acggtttttt acaatctatt
     1741 tacaactcta tgatagctgt cacgattaat tccatgcaag catgatgcga gtcactctta
     1801 ccaaattttt caaaatcata gaatctacct taaaggagaa tatgatgaaa cgaacgattg
     1861 ccctcactct tagtttgtta tggctcacag catgtgtaac tctgacgaat cctgcgcctc
     1921 ttattccaac agaaactgtt ccaacaaccc caagtgtctc tgcagccgac aatactccca
     1981 catacgtgcc gcttatcaat aacagcgcat tactctatat ttccggcact actcacattg
     2041 aatccaagcc gcaatcatgg ccaaatatag atgcatttct aaatttcctt caaaacatta
     2101 cagcacaagg tttgaaatgg tcagttggag cagatatagg ctggctagaa ggagaaccgc
     2161 gtgctgctga attgatccaa gcctctgaag cattgggggt gcaatgggat atccatgccc
     2221 ataacccaca agagcgtgcc aaagcagctt atctcatcac tcaattaggc gggcacccta
     2281 cttcagtcat atctgggatg cttgtcagtg aattcagtag catcggacct caaacatacc
     2341 aaggtttcac atggactcca cacgtgttat ggggtggcac gaactgtccc ggtcaccgcc
     2401 caggatgtga tgatctagcg gcaggcatct ggattccgct tagtgctgag caatacacca
     2461 ctcacgaccc caatggcaca ctcatccgcg ttggcggtgg ctctcatcaa ttagccgatg
     2521 ggctagttct cgccgaatct atcgcaaata gacaatatcc atatccaatt atcagtttca
     2581 cattaatggt ctctcccacc tctctaacca tagtcaacag caatgatagc ttagaacaaa
     2641 tcacagcatt tgcaaacgat atgaagcagt atccctttgt atattgggca tatattgaag
     2701 agacagctca agcttggact aaagccggaa aggttcccag tcgaattgag tgaaataaaa
     2761 acaggtcacg t
//
LOCUS       DAOQFT010000340         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22516_length_2770_cov_5.135175, whole genome shotgun sequence.
ACCESSION   DAOQFT010000340 DAOQFT010000000
VERSION     DAOQFT010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22516_length_2770_cov_5.135175"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLR93_09005"
     CDS             <1..218
                     /locus_tag="PLR93_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27145.1"
                     /translation="TSDPNGTTGHTVEITCTFPDGSTDIIDNGEYAIASIGGMFGIGA
                     LIGLLISLPLFVVPFFLIRKRKEPSAL"
     gene            complement(263..1198)
                     /locus_tag="PLR93_09010"
     CDS             complement(263..1198)
                     /locus_tag="PLR93_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH27146.1"
                     /translation="MSDLKRLMPFMVSILVVLACAVPSIPIRNADTMNTNVANTVIAG
                     LTQSVTATFPASSTPSETLAYTSTPTLTYLTARVPSPTLTFTPLPGTDTAIPLLTDTA
                     EPGLVKISVTRPTHCRSGPGKSYEIVGSLLVGMKADVIGRDPTSEYWYIPNPYVFTDY
                     CWVWGEYAEFEGNQLAVPVVMPPPTPTSTATTIPTVDFKLLGSGHHSCDKTHWMNITI
                     LNESNFTFSSINIVMQDKVKNISRSVTYNNFPIQTGCEGLTWVETVPADASATVLGPK
                     FDYNIRGNKLHVTVTVCSEKDMKGACSTRETNLVP"
     gene            complement(1237..2046)
                     /locus_tag="PLR93_09015"
     CDS             complement(1237..2046)
                     /locus_tag="PLR93_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27147.1"
                     /translation="MKRFFSKKPEGQPTRGKTADNRLRRVDNFVLLYEPSLLTRTDSL
                     FAKSLFVDLGYGFDARTTLESAVRFRKVNPNLNILGVEIDKERVEAALPYADDKTFFR
                     LGGFNLPLQSGETVRLIRAFNVLRQYEERDFAPAYERLAEYVLPGGLLVEGTSTPFGS
                     LWAANVARRAESAQWSFEALVFSTNFHTGFDVEEFQTILPKNYIHRMVKGELIFEFFE
                     AWKRSAAETSHTKIFGLKQWFIASAEALGQKGFQVDRHKKWLNKGYLIWYL"
     gene            complement(2106..>2770)
                     /locus_tag="PLR93_09020"
     CDS             complement(2106..>2770)
                     /locus_tag="PLR93_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNH27148.1"
                     /translation="DRKVIVSYEQREEAILDIPNTSRILVRDGSHVEAGTPLTEGSLN
                     PHRILKIQGRDACQMYLMTEVQKVYRSQGQNIHDKHFEVIIRKMLSKVQVTRPGDTKY
                     LPGDVVDRLEIRKTNEALIAEGRQPARFSEVLLGVTKASLSTDSFLSASSFQHTIKVL
                     ASAAIGSTTDPLYGLKENVIIGKLIPAGTGFIHGRFTPPGGEETAAPDSAEEMPDTTA
                     GD"
BASE COUNT          738 a          671 c          649 g          712 t
ORIGIN      
        1 ccacttcaga ccccaacggg accacaggtc acaccgtgga aatcacctgc accttcccag
       61 acggctctac cgatatcatc gacaatggcg aatacgcaat tgcctccatc ggcggcatgt
      121 tcggtattgg ggcattgatc ggactgttaa tttcattacc tttgtttgtg gttccgtttt
      181 tcttaattcg caaaagaaaa gagccatcag ctttatagca tctggctcta aacacataag
      241 gcacttcata cttctttgtt aactaaggca ccaaattggt ctcacgggta ctacatgcac
      301 ctttcatatc cttctctgaa cagacagtaa ctgtcacatg cagtttatta ccacggatgt
      361 tgtaatcaaa tttaggacca agcacagttg ccgaagcatc tgcaggaacc gtctccaccc
      421 aagtaagccc ttcacaacct gtctgaatag ggaagttgtt gtacgtcaca gagcgagaaa
      481 tattcttgac cttatcttgc atcacaatat taatggagct aaacgtaaaa ttactctcat
      541 tgagaatcgt aatattcatc caatgtgtct tatcgcaaga atgatgtcca ctgccaagta
      601 acttaaagtc aacagttgga atcgttgttg cggtgctggt gggtgttggt ggcggcatga
      661 caacaggaac cgctaattga tttccttcaa actcggcata ttctccccat acccaacagt
      721 aatctgtaaa cacatatggg ttcgggatat accaatattc agatgttggg tcacgcccaa
      781 taacatctgc tttcattcct accagaagcg aaccaacaat ttcataagat tttcctggac
      841 cagaccggca gtgggttggg cgagtcacac tgatctttac caaacctggt tctgctgtat
      901 cagttaatag tggaattgca gtatccgtcc caggcagtgg agtaaaggtt agtgttggag
      961 aaggcacacg tgctgtaaga tatgttaatg tcggggtgga tgtatatgcc agtgtttcag
     1021 aaggtgtact actagctggg aatgtagctg ttacactttg cgtcaacccg gcaatcacgg
     1081 tgttagcgac attagtgttc atggtatccg cattccttat gggaatacta ggaacagcac
     1141 aagccagaac aactagaatt gaaaccataa atggcataag tctttttaaa tcagacatag
     1201 atgtctccta ttacattccc aattatgaca cgaaaatcaa agataccaga tcaagtaccc
     1261 tttgttcagc cattttttgt gacgatccac ttgaaaacct ttttgcccca acgcctcagc
     1321 agaggcaata aaccattgct ttagcccaaa aatcttggtg tgtgatgtct ctgcggctga
     1381 gcgtttccat gcctcaaaaa attcgaagat caactcacct tttaccatac gatggatata
     1441 gttcttggga agaatcgtct gaaattcttc gacgtcaaac cccgtgtgga aattagtgga
     1501 gaagaccaac gcctcaaacg accattgagc ggactctgct ctccgagcca cattcgccgc
     1561 ccaaaggcta ccaaacggcg tggatgtccc ttccacgaga agaccgcccg gcaaaacata
     1621 ttccgcaagg cgttcataag cgggggcaaa atccctctcc tcatattggc gcaatacatt
     1681 gaatgcccga atcaaacgca ctgtttcacc actttgcaat ggcaaattga agccacctaa
     1741 acgaaagaaa gtcttatcat cggcatacgg caatgccgct tcaactcgtt ccttatcgat
     1801 ctccacgcct aaaatattta gatttggatt taccttccga aagcgaacgg cgctctcaag
     1861 tgtggtgcgt gcatcaaagc cataaccgag atccacgaac agggacttgg caaacaggct
     1921 atcggtccgg gtgagaagcg aaggttcata gagaagcaca aagttatcca cacgtcgcag
     1981 gcgattatca gcagttttgc cacgtgtggg ttgtccctct ggttttttag aaaagaaacg
     2041 tttcatcaaa acgattatac aaaataaaaa cgccccgaat tttcattcga ggcgtttcgg
     2101 tttacttagt cgccagcggt ggtatctggc atttcttcgg cagagtctgg agcagccgtt
     2161 tcttcaccac cgggaggggt aaagcgcccg tggatgaagc ccgtacctgc agggatgagc
     2221 ttaccgatga tgacgttctc cttcaagccg tagagcgggt cggtggtgga accgatggca
     2281 gcgctcgcca acactttgat ggtgtgctgg aaggacgagg cagagaggaa ggagtcggtg
     2341 gagagagaag ccttggtgac accgagcaac acttcgctga agcgagctgg ttgtcttcct
     2401 tccgcgatca acgcttcgtt ggtcttgcgg atctcaagac gatccaccac atcaccaggc
     2461 aagtacttgg tatcgccggg gcgagtgacc tgcaccttgc tcaacatctt gcggatgatg
     2521 acttcaaagt gtttatcgtg gatgttctga ccttgtgagc gatacacctt ctgcacttcg
     2581 gtcatgaggt acatctggca ggcgtcgcgt ccttgaatct tcaagatgcg atgcgggttg
     2641 agagaacctt cggtgagcgg tgtgcctgct tccacatgcg agccatcacg gacgaggatg
     2701 cgggacgtat tcggaatatc gagaatggct tcttcgcgct gttcgtaaga gacgatgacc
     2761 ttgcgatctt
//
LOCUS       DAOQFT010000341         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22542_length_2768_cov_6.454110, whole genome shotgun sequence.
ACCESSION   DAOQFT010000341 DAOQFT010000000
VERSION     DAOQFT010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22542_length_2768_cov_6.454110"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..577)
                     /locus_tag="PLR93_09025"
     CDS             complement(92..577)
                     /locus_tag="PLR93_09025"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNH27149.1"
                     /translation="MKRPNFIQKFVHRFFMLKPVTAFFATRTHIMDGIALKLTGGKYT
                     VSEILGWNIAQIITTGAKTGEKHTIILIAVINGEKIALIGSNFGRKPNPGWYYNLKKH
                     PECEVHLNGKAGMYLARETEGEERESYWKQAVALYAGYEKYKERASHRRIPVMLLEPI
                     K"
     gene            complement(577..1014)
                     /gene="dtd"
                     /locus_tag="PLR93_09030"
     CDS             complement(577..1014)
                     /gene="dtd"
                     /locus_tag="PLR93_09030"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNH27150.1"
                     /translation="MRVLIQRVSQASVTVEQQTISSIGKGLLILLGIGHGDGEEQVQF
                     LAEKTANLRIFEDEQGKTNLSVLDVKGEAIVVSQFTLYADSRKGRRPSFIDAALPDVA
                     EPLVHRFVELLRSHGVPTQTGKFGAHMQVEIHNDGPVTIWLEK"
     gene            complement(1052..2038)
                     /gene="trpS"
                     /locus_tag="PLR93_09035"
     CDS             complement(1052..2038)
                     /gene="trpS"
                     /locus_tag="PLR93_09035"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNH27151.1"
                     /translation="MAKKGRVFSGARPTGRQHLGNYLGAIENYVGLQTDYDCVYCIVD
                     VHALTTVETTQELRQNTFEMALDWLAAGIRPEDSILFVQSHVPEVMELHTYLSMVTPL
                     GKLTDLPTFKEKVRQQPENVNYGLLGYPVLMTADIVLYKTDIVPVGIDQAPHIEFARE
                     TVRSFNYRFGTKALIEPQVKHTEVKKVVGIDGKDKMSKSLNNHIELALTAEETTKRVR
                     EMVTDPARQRKTDPGNPDVCNVFTMHKIFSPQEEVDMINTECRKAGIGCVDCKLRFAS
                     NLNKHLEPFRAKRADFASKPAEVQEILNDGGKRARAIAQETMSEVREAMKLP"
     gene            complement(2095..2283)
                     /locus_tag="PLR93_09040"
                     /pseudo
     CDS             complement(2095..2283)
                     /locus_tag="PLR93_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            2528..2767
                     /locus_tag="PLR93_09045"
     CDS             2528..2767
                     /locus_tag="PLR93_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH27152.1"
                     /translation="MPVYTYRCENCGVQFERQQSFTDDPLKTCPECRKKALKKVITPS
                     KVIFKGSGFYATDHKSPSGDSSSSKKSTESSSSAE"
BASE COUNT          679 a          703 c          652 g          734 t
ORIGIN      
        1 cttgctttga gccgttgtct tcttccttcc gcaacgaaaa cctcttgcat ccttcaagcc
       61 ttaatttatc attaaattcc aaagttggca attattttat aggctctaag agcatgaccg
      121 ggatgcgacg gtgtgaggct ctttctttgt atttttcata gcctgcatac agcgcaaccg
      181 cctgtttcca ataggactcg cgctcttctc cctcggtctc gcgtgccaga tacatccccg
      241 ctttcccgtt caagtggact tcgcattcag gatgtttttt gaggttgtag taccagccgg
      301 ggttgggttt gcgcccaaag ttagagccaa tgagagcaat tttttcaccg ttgatgacag
      361 cgattaaaat gatggtgtgt ttttcgcctg tcttcgcgcc agtggtgatg atctgggcaa
      421 tattccaccc caaaatttca gaaaccgtat actttccgcc tgtgagttta agggcaatcc
      481 cgtccatgat gtgggtgcgg gtggcaaaga aagctgtgac cggtttcagc ataaaaaaac
      541 gatggacaaa cttttgaata aaatttgggc gtttcattac ttctccaacc agatggtgac
      601 aggtccatca ttatgaattt cgacctgcat atgggcgccg aacttgcctg tttgagtggg
      661 aaccccatgt gaacgtaaga gttctacaaa gcgatgcacc agcggttcgg caacatcggg
      721 caaagcagca tcgataaaag aggggcggcg tcccttacgg ctgtcggcat agagcgtgaa
      781 ctgtgaaaca acaatcgctt caccttttac atccaaaacg gagaggttgg tcttaccttg
      841 ttcatcttca aagatgcgca agttggcggt cttctcagca aggaattgaa cttgctcttc
      901 cccatcgccg tgaccgatgc ccaataaaat cagcagacct tttccgatgc tggaaatggt
      961 ctgctgttct acggtgacgc tggcttgaga aactcgttgg ataaggacgc gcataagaaa
     1021 taaatagggc ggggaaaccc cgcccctgcg tttaaggtaa cttcatcgcc tcgcggactt
     1081 cgctcatggt ttcttgagca atggcgcggg cgcgtttgcc gccatcgttg aggatttcct
     1141 gcacttcggc aggtttagaa gcaaagtcgg cgcgtttggc gcggaagggt tcaaggtgct
     1201 tgttgaggtt agaagcgaag cgcagtttgc aatccacaca gccaatgcct gctttgcggc
     1261 attcggtatt gatcatgtcc acttcttctt gtggtgaaaa gatcttgtgc atggtgaaca
     1321 cgttgcatac atcagggttg ccggggtcgg tcttgcgctg acgagccggg tccgtgacca
     1381 tttcgcgcac gcgtttggtg gtctcttcgg cagtgagcgc caactcaata tgattattga
     1441 ggctcttgct catcttatcc ttgccgtcga taccgaccac ttttttcact tcagtgtgct
     1501 tgacctgtgg ttcaatcaag gcttttgtgc caaaacgata attgaacgag cggacggtct
     1561 cgcgcgcaaa ttcgatatgc ggagcctggt caatgccgac cgggacaata tccgtcttat
     1621 acaaaacaat gtccgccgtc atcaaaaccg ggtaacccag caaaccatag ttgacatttt
     1681 ctggctgttg gcgaactttc tctttgaagg tcggcaaatc ggtcaactta ccaagcggag
     1741 tgaccatgga aagataggtg tgtaattcca tcacttcagg cacgtgtgat tgcacaaaca
     1801 atatcgaatc ttccggtcga atccctgctg cgagccagtc gagcgccatt tcaaaggtgt
     1861 tctgacgaag ctcctgtgtc gtttccacag tggtcaatgc atgaacatcc acgatgcaat
     1921 acacacaatc atagtcagtt tgtaagccta cataattctc aattgcgcca aggtaattcc
     1981 ccaaatgttg gcgtcccgtt gggcgcgcgc cagaaaatac tcgtcctttt tttgccattc
     2041 gtatgtcctt gttatccgca agggagacct agttgggtca cccctacatg taatttaatc
     2101 aaccatcttt gatatcaaac cgagcacttc gcctgcttct gcatgacgct tagtctgcaa
     2161 tttggagtag accaactccc cgttcacgct gacctcgaac ctgcctccgt ccgaaggaac
     2221 aagagtgatc ccctcgatga cgtgctcata atctttcaac aattcctgtg ccaagctcac
     2281 ggctcggttg gtgtaatgtc aaaccgcaca atagacgatc tcaatcttta gcatagttgc
     2341 tccacaagac cttcacccta tagactgcaa cttttcttgt cccctcacat ctaacaaggt
     2401 gccaggtcag cgaaattgta agaaaattat aacatgctgc ttctgaaggc ttgccacacc
     2461 ctaacaaggg ggctataatg ccgcagttgt atccaagaaa tattagtaag aagcaaggag
     2521 agaacgtatg cccgtctaca cttatcgatg cgagaattgc ggagtgcaat tcgaacgcca
     2581 gcaatccttc accgatgatc cattgaagac ctgccccgag tgccgcaaga aagcactgaa
     2641 aaaggtcatc acccctagca aagtgatttt caagggttca gggttctatg ccaccgacca
     2701 caagtcacct tctggagatt cctcttcttc caagaagagc accgaaagct cctcctctgc
     2761 tgaataag
//
LOCUS       DAOQFT010000342         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22553_length_2767_cov_5.906711, whole genome shotgun sequence.
ACCESSION   DAOQFT010000342 DAOQFT010000000
VERSION     DAOQFT010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22553_length_2767_cov_5.906711"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..656)
                     /locus_tag="PLR93_09050"
     CDS             complement(150..656)
                     /locus_tag="PLR93_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27153.1"
                     /translation="MKKTLVIVALVMTALAILGVGAVFAQDVTPPYAGHGPMMNGEGP
                     LHTFMVIEFAKKLDLNVNDINTRLDAGESMYDIALSTGLTAEEFPAVMTEVRTNAVNA
                     AVAANVITQEQADWMLSHGYGQGGMHGNGTGTCDGTGPQGQAGYGHGMMNGGGMMGGR
                     WQNQQTNP"
     gene            complement(818..1075)
                     /locus_tag="PLR93_09055"
     CDS             complement(818..1075)
                     /locus_tag="PLR93_09055"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNH27154.1"
                     /translation="MTDKLDALLEQVHAELQKVDEVDADSLKLLQELQQDVQGLLDKT
                     EMETPTVLARMQKAVQQFEVDHPALTALISELSTVLSNAGI"
     gene            complement(1164..1424)
                     /locus_tag="PLR93_09060"
     CDS             complement(1164..1424)
                     /locus_tag="PLR93_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27155.1"
                     /translation="MAKLIPASERILRARKLIEQARALPVPTEGLGKSDFSYIANVRD
                     VLRQAKDMVKFIPQTAGVSLEMKEEVKKIYAEVEQAEKEILS"
     gene            complement(1434..>2767)
                     /locus_tag="PLR93_09065"
     CDS             complement(1434..>2767)
                     /locus_tag="PLR93_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27156.1"
                     /translation="TPLPSEPLIDSSTQAAFAPQATALPIGTSTPTSLPKGVIFVDTL
                     QQEVYPFEENGKCSLAEAVIAANTGAPKDTCPAGVPGESIISLMPGEYHFSQRDQSPP
                     QFDWLLSLVKVGSALPPITFPLVIQGNGATLVRDEGSEPFRFFEVMVNASLSLENMTL
                     QNGDVQDDSGGAIYASNGSVTLNRVRFLNNHAEHGGAVYLTFGALTVQESEFIENHAG
                     FQGGGIHLDSAKSTIQNTKFISNTTDARGGALSAEGVTLVVEDSLFLKNVTQDNRGGA
                     LHLEHVNVNITRSQFYQNQSAWIGAAIYINNPVMSGTSEDEGDPLEQLDDTPMYVQMA
                     TLIPGYEATLQAHPSGVFQDFHEDMQVHQNCFANNIILDTYEMNFSSAILARDVNAED
                     NYYGTASGPSGSGPGTGDGVGRSVKFAPFLTEPPAYCDPELSQQVKENHNQ"
BASE COUNT          683 a          691 c          687 g          706 t
ORIGIN      
        1 tgcaatgcag gatgaaatca tgcatatggg cgggtatgac atcgtcatga atggtatctg
       61 gacgggcgga gatgtgaaaa agtatttgat agaaggaaaa gagtaaacaa aaagagacgg
      121 cttgcaccgt ctctttttgt ttgcagtgat tacggattgg tctgttgatt ctgccagcgt
      181 ccgcccatca tgccgccgcc attcatcatg ccgtgaccgt atccagcctg cccttgtgga
      241 ccagttccgt cacaagtgcc agtcccgttg ccgtgcatgc caccctgacc atatccatga
      301 gacagcatcc aatctgcctg ctcttgggtg atcacattgg cagcaactgc tgcgttgaca
      361 gcgttggtgc gaacttcagt catcacagct gggaattctt cagcggtcag cccagttgag
      421 agggcaatat cgtacatgct ttcaccagca tccaggcgag tgttgatgtc gttcacgttc
      481 aagtcaagtt ttttggcaaa ttcaatcacc atgaaggtgt gaagcggacc ttccccattc
      541 atcatgggtc catgcccagc atacggcggg gtgacgtcct gagcaaaaac cgcacctact
      601 cccaaaattg ctagcgctgt cataaccaac gcaacgatca caagtgtctt tttcatttta
      661 tttctccttt tcgttttcga acttacaagc caaagtgtac agcccgcatg taaaggttct
      721 gtaaagaaaa ggtagaggtc aggtgaagaa ttccgatcat caaaaataaa aaagaccgtc
      781 atgaaggaaa attccttcat gacggtctcg ggctctttca aattcccgcg ttgcttaata
      841 ctgtggatag ttcagagatc aaagccgtaa gagcagggtg atctacttca aattgctgca
      901 ccgccttttg cattcgggca agcacggttg gcgtctccat ctcggtcttg tcgagcaatc
      961 cttgcacatc ctgttgcagt tcttgtaaaa gtttgaggct gtcggcatcc acttcgtcca
     1021 ctttttggag ctcggcatgg acttgttcca gaagtgcgtc gagtttatcg gtcatggggt
     1081 tggctccttt cgaggatcag gttcactttc attgtacaac caacccaagg ctgcttccag
     1141 tgaataacgt cataaaacct tgattatgac aatatttcct tctctgcctg ttccacttca
     1201 gcgtagatct tcttgacctc ttccttcatt tcgagcgaaa cacccgccgt ctgtggaatg
     1261 aacttgacca tatcctttgc ctgacgcaaa acgtcgcgga cattggcgat ataggaaaaa
     1321 tcgcttttcc caagaccttc tgtcgggacg ggtaaagccc gggcttgttc gatcagtttt
     1381 cgggcgcgca agatgcgttc agatgcaggg ataagttttg ccatgatgaa atcctattga
     1441 ttatgatttt ctttcacttg ctgtgacaac tcagggtcgc aataggcagg cggttcggta
     1501 aggaagggtg caaatttcac agaccgacct accccatctc cggtgcctgg accactgccg
     1561 gagggtccgc tagcggtgcc gtagtaatta tcctcggcat tgacgtcacg cgcaaggatg
     1621 gcgcttgaga agttcatctc ataggtatcc aaaataatat tattggcaaa acaattctga
     1681 tggacctgca tatcttcatg aaagtcttga aacaccccac tggggtgagc ctgcaaggtc
     1741 gcttcatagc caggaataag agtcgccatt tgaacataca taggcgtatc atccaactgt
     1801 tcgagcggat ccccttcatc ttcgctggta ccgctcatca ccgggttgtt gatgtagatc
     1861 gcggctccga tccatgcaga ctggttctgg taaaactgac tgcgcgttat attgacgttg
     1921 acatgttcca aatgcaaagc gccgccacga ttatcttggg ttacattttt caagaacaaa
     1981 ctgtcttcaa ccaccaaggt aaccccctcg gcagaaagcg caccaccgcg cgcgtcggtg
     2041 gtgttgctga tgaatttcgt attctgaatc gtggacttgg cagagtcgag gtgaataccg
     2101 ccaccttgaa agccagcatg attctcaata aactcgctct cttgaacggt cagtgcccca
     2161 aaggttaaat agacagcccc gccatgctca gcatgattgt tcaaaaaacg gacgcggttc
     2221 aaggtgacag acccatttga ggcgtagatt gcaccgcccg agtcgtcttg tacgtcgcca
     2281 ttttgcaaag tcatattctc aaggctgagg ctggcattga ccatgacttc aaagaagcgg
     2341 aagggttccg agccttcatc gcggacaagg gtcgcgccgt taccttgaat aaccaatggg
     2401 aaagtaatcg gcggcaaggc gctgccaacc ttgaccaagc ttagcaacca atcaaattgc
     2461 ggcggacttt ggtcacgttg actgaaatga tattctccgg gcatgaggct gataatggat
     2521 tcacccggca caccagcggg acaagtgtct ttaggtgcgc ctgtgttggc agcaatgaca
     2581 gcctctgcca gtgaacattt accattctct tcgaagggat acacttcttg ctgcaatgta
     2641 tcaacgaaga taaccccttt gggcagagaa gttggcgtgg acgtgccgat gggcagagcc
     2701 gtcgcttgcg gggcaaaagc agcctgggtg gatgaatcaa tcagcggctc agaaggaagc
     2761 ggggtac
//
LOCUS       DAOQFT010000343         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22601_length_2763_cov_7.131462, whole genome shotgun sequence.
ACCESSION   DAOQFT010000343 DAOQFT010000000
VERSION     DAOQFT010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22601_length_2763_cov_7.131462"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLR93_09070"
     CDS             complement(<1..611)
                     /locus_tag="PLR93_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27157.1"
                     /translation="MFTRLEKLYHQFPPRYWVIVLTFFIDSIGGTLLFPFFSLYITQK
                     FNVGMTQAGMVLAMFSIFGIVGNMIGGALTDRLGRRKLILFGLVFSALSTLSLGLVNS
                     LAVLFPLAAFIGLLSDIAGPANQAMMADILDEKQRQEGFGLMRVIRNLAWIIGPSIGG
                     FLANRSFMLLFIIDAVVSCIVAVIFFTYMPETKPQHHEEEHKE"
     gene            complement(701..1051)
                     /locus_tag="PLR93_09075"
     CDS             complement(701..1051)
                     /locus_tag="PLR93_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27158.1"
                     /translation="MKVISIFLALINFLTGVLLILSCISANETFTWVAWKTGLGVLGV
                     TFGILTFKDSAQPVSQRKMILYGLLLVVVGVSIVAYGIHWSVISGDPKHTVMVVGSSF
                     FLHGFTSVLGMASD"
     gene            complement(1118..1390)
                     /locus_tag="PLR93_09080"
     CDS             complement(1118..1390)
                     /locus_tag="PLR93_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27159.1"
                     /translation="MKNGTCPKCGSNEILSELPLHGGEGHPSYVDIVQPEPPNRPFIW
                     VAQSEQSKFVAYICGACGYSEFYAVRHQALNEGRKKGFKPRFDYTA"
     gene            complement(1471..2535)
                     /locus_tag="PLR93_09085"
     CDS             complement(1471..2535)
                     /locus_tag="PLR93_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH27160.1"
                     /translation="MKRGILLLIGILLLFSALIAGYILTQTYNFNGYVQPEPIKAPET
                     MLRSVNGPVRLSDFQGKIVLLYFGYTFCPDVCPTSLANIKLALADLTPEEAAQVQVIF
                     VSVDPARDTPEALGQYVNMFQDNFIGATGTRDEIDWMVNAFGAKYTIESPDVNGNYNV
                     SHSAFTMVIDRNGFYVLNWAHGTPSTDMSADLHHIINKGIPVSAQILAGPTYTPVVCA
                     VTLIPSHVQNGQWLYEHHCAQCHGMDLAGNPQWQVELADGSHLAPPLNGSGSAWKYSR
                     TDLLAIIKEGRNLEKPIHMPAFKSNLADWEIDFILTYIQSTWDINQLNYQHGFMTLTP
                     NPTSTPAAPLAPLVTVTPSP"
BASE COUNT          714 a          617 c          791 g          641 t
ORIGIN      
        1 tgttctttat gttcttcttc atgatgttgc ggtttggtct ctggcatgta ggtgaagaag
       61 ataactgcca cgatacagct cacaacggca tcgatgataa agagcagcat gaaggagcga
      121 ttggcaagga acccgccgat gctcggaccg ataatccatg ccaggttgcg gatgacgcgc
      181 atcagtccga atccttcttg acgttgcttt tcgtcgagga tatctgccat catggcttgg
      241 ttggctggac cggcaatatc tgaaagcaga ccgataaatg cggcgagcgg aaataagact
      301 gctagcgagt tgaccaaccc gagcgacagt gtgctcagtg cgctaaagac caagccgaag
      361 agaatcaact tccggcgacc aagtcggtct gtgagggcgc cgccaatcat attgccgacg
      421 atgccgaaga tggaaaacat ggcaagtacc atgccagctt gcgtcatgcc gacgttgaat
      481 ttttgggtaa tatacaaaga gaagaagggg aagagcagcg tcccgccgat gctatcaata
      541 aagaaggtga ggacgatgac ccagtagcgg ggaggaaatt ggtgataaag tttttcgaga
      601 cgtgtgaaca tctcgcaata atagcacgtt tgttctgaat taggaaagag atttttcttt
      661 tgcggcagag agcagagcgg ggaagaactc tgcggtgaga ttaatcgctt gccatgccta
      721 agaccgaggt gaacccgtgt agaaagaaac tactgccaac caccatgacg gtatgctttg
      781 ggtcaccgct gatgacagac cagtgaatgc catacgccac gatggaaacg ccgaccacga
      841 ccaagagcaa gccatacaaa atcatcttgc gttgactgac aggctgtgca ctatctttaa
      901 aagtgagaat gccaaaggta acacccagaa cgcccaaccc ggtcttccag gcgacccagg
      961 tgaaggtctc attggcagaa atgcaggaga gaattaacaa aacccctgtg aggaagttga
     1021 ttagcgccag aaaaatagag atgactttca tacccgtgcc tccttgtgtt gaggtcagga
     1081 tagcagggta gggctgtcaa agactattga gagtctgtca ggcagtgtaa tcgaagcgcg
     1141 gtttgaagcc tttttttcga ccttcgttta gagcttgatg gcgaacggca taaaattcag
     1201 aatagccgca agcaccacaa atatatgcca caaatttact ttgttcgctt tgcgccaccc
     1261 aaatgaaggg acggtttggc ggctctggtt ggacgatatc cacgtatgat ggatgccctt
     1321 cgccgccatg taagggaagt tcacttaaga tttcgttcga gccgcatttg gggcaagttc
     1381 cgtttttcat gatgaagctc ctaaaggatg agatgcactc atcatagcct gtgggtttgt
     1441 aaaggagtat ccgccaaagg gtggagaaaa ttatggagag ggcgtcaccg ttacgagcgg
     1501 ggcaagaggc gcggcagggg tgctggttgg gttgggagtg agggtcatga atccgtgttg
     1561 atagttcagt tggttaatat cccaagtgct ttgaatatag gtgaggatga agtcgatctc
     1621 ccaatcggcg aggtttgatt tgaaggcagg catgtgaata ggtttctcga ggttgcgtcc
     1681 ttctttgatg atggcaagca ggtcggttcg agaatacttc cacgcgcttc cactgccatt
     1741 gagcggggga gccagatgcg agccatccgc caactctacc tgccactgcg ggttgccagc
     1801 caagtccatg ccgtgacatt gtgcgcaatg atgttcatac agccattgac cgttttggac
     1861 gtgcgacgga atcaacgtga ctgcacaaac gactggcgta taggttggac ccgccaaaat
     1921 ttgcgcactg accggaatgc ctttgttgat gatgtgatgc aggtctgcgc tcatatctgt
     1981 agacggtgtg ccatgcgccc aattcaacac gtaaaagccg ttgcggtcga tcaccattgt
     2041 gaacgccgag tgactgacgt tgtagttccc attgacatcg ggcgactcga tggtgtactt
     2101 ggcgccgaag gcattcacca tccaatcgat ctcgtcacgc gtgccggtgg cgccgatgaa
     2161 gttatcctga aacatgttga catactgacc gagcgcttcg ggcgtgtcgc gagctgggtc
     2221 tacagaaacg aatatgacct gcacctgagc cgcttcttcc ggggtcaagt ccgcgagcgc
     2281 cagcttgatg ttcgccaatg aggtggggca aacatctggg cagaaggtgt agccgaaata
     2341 cagtaacaca atcttgccct gaaaatcgct caagcggacc ggtccattta cggagcgtaa
     2401 catggtctca ggtgctttga tcggttcggg ctgaacatag ccgttgaagt tgtaggtttg
     2461 ggtgaggata tagccagcga taagggctga gaataataac aagatgccga ttaagagaag
     2521 gatgccacgt ttcataagaa ctccgcacaa gatttagtag ggcggggttt ccccgcccaa
     2581 tcaaatctta gtaacgaggg cgaggggacc tcgcccctac gtttcgattt atacctttag
     2641 cgtggagttc tgtctttgcg ctggcgcaag cattgtgcag gcgttttgac ggtcaaaatg
     2701 gggatattag gcagtcgaag gagagtcgaa cgagtgtcga aggatggata gaggatagta
     2761 aga
//
LOCUS       DAOQFT010000344         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22883_length_2742_cov_4.594715, whole genome shotgun sequence.
ACCESSION   DAOQFT010000344 DAOQFT010000000
VERSION     DAOQFT010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22883_length_2742_cov_4.594715"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLR93_09090"
     CDS             complement(<1..384)
                     /locus_tag="PLR93_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27161.1"
                     /translation="MSINDIISQILIFINSNPPLVLALFVLSLIANLIQVGTYFRDRQ
                     KLKSEEVERHKLAEMVNSYEDVLKIAKETVQDKTKLSQLKDEIRESASEAMRLTQEIS
                     QIEKMAQRKLVSQAIEYNLGVLNRAY"
     gene            complement(562..>2742)
                     /gene="polA"
                     /locus_tag="PLR93_09095"
     CDS             complement(562..>2742)
                     /gene="polA"
                     /locus_tag="PLR93_09095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNH27162.1"
                     /translation="TLDAIYANLDQVENRWRGKFETNKETAYMSRDLAQIETNLKIKF
                     DFEHAKVAPFDGSKLEAFFKEMEFRTLVSKVPAISGGVASAPAETKAPAVKTGKTKAG
                     QMTMFAEEAQPVAVAIKPASIEVVIVDSAEKLKSLANELEKAKVISFDTETTGTEEMT
                     AELVGISLAVKEGQGYYIPVGHVAGENLPLQTVIDAITPAMTNAKIGKVAHNAKYDYI
                     VLARYGLTVSPLTFDTMLAEFIVDPASRHLGLKNLSEHRLGEEMTHIEELIGKGKKQI
                     SMAEVAIEAVANYAAADAETTLRLMAIEEKELKRVEGQKILDEIDMPLTAVLAEMEMT
                     GALIDTKFFGEMSKELAARLNEIEKEIYGHVGKTFNINSPQQLSDVLFNHLRLEPPDK
                     GRKTSTGFYSTSADVLDSMRGQHPVLDFILEHRELSKLKSTYVDALPAAVDANTGRVH
                     TSYSQIGAVTGRLSSNNPNLQNIPIRTETGRKVRNGFIADKGNLLLSVDYSQIELRIV
                     AHMAQDEAMLAAFRAGEDIHATTAAAVYDIPLEQVNKDMRRHAKAINFGLIYGMSAFG
                     LMHSTGLTLAESEDFVKAYFKKFPGIKKYLDGIRRDAAEIGYVETLLGRRRYFPALQT
                     KTNVQLKNREEREAINAPIQGTAADIMKIAMLNIPPALQKAGLKAKMLLQVHDELVLE
                     VPKKEIEQTAKLVQETMANAYPMSIPLETEARAGVSWGEMTVLE"
BASE COUNT          595 a          767 c          621 g          759 t
ORIGIN      
        1 atacgcacgg tttaagactc ctaaattgta ttcaattgcc tgcgacacca atttcctttg
       61 agccattttt tcaatttgac ttatctcttg tgttaatctc atcgcttccg atgcggattc
      121 tcttatttca tccttcaatt gggaaagttt tgttttatct tgaacggttt cttttgcaat
      181 tttgagaaca tcttcgtaag aatttaccat ctcggctaat ttgtgccgct cgacttcttc
      241 gctttttaat ttctgacggt ctctaaaata agtccctact tggattagat ttgcgataag
      301 acttaataca aacaaagcca agaccaaagg ggggttactg tttatgaaaa taagtatctg
      361 ggaaataata tcatttatcg acattgtgga attactccat cgttatagca gcccaactag
      421 gttttataca gcactatgct gtatatactc ccaatttggg gttttataca gcatcatgct
      481 gcataatccc ctttattttg cataacgtca ttatacaagt cccgccaaaa aggggcaaga
      541 aaaccggctt gccccttcct attattccaa caccgtcatc tcgccccagc tcacaccggc
      601 acgcgcttcg gtctccaacg gaatgctcat tgggtaggcg ttcgccatcg tctcttgcac
      661 aagcttggcg gtctgctcga tctctttctt cggcacttcc aacaccaact catcatgcac
      721 ttgcaacagc atcttggctt tcaagcccgc cttctgcaaa gccggtggga tgttcaacat
      781 cgcgatcttc atgatgtctg ccgccgtgcc ctgaatgggg gcgttgatgg cttctcgttc
      841 ttcgcggttc tttaactgga cgttggtctt ggtctgcaag gctgggaagt agcgcctccg
      901 cccaagcagg gtttccacat acccgatctc cgccgcatcg cgccgaatgc catccaaata
      961 tttcttaatg cccgggaatt tcttgaaata cgctttcaca aaatcttcag actcagccag
     1021 cgtcagcccc gtcgaatgca tcaacccaaa agcagacatg ccatagatca acccgaagtt
     1081 gatcgccttg gcatgccggc gcatatcctt gttgacctgc tccagcggaa tatcgtacac
     1141 cgccgccgcc gtcgtggcgt gaatatcttc gcccgcgcgg aacgccgcca acatcgcttc
     1201 atcttgcgcc atgtgcgcca caatgcgcag ttcgatctgc gagtagtcca ccgagagcag
     1261 caaattgcct ttatcggcaa tgaagccatt gcgcactttt cgccccgtct cagtgcggat
     1321 ggggatgttc tgcaagttcg ggttgttgga agagagccgc cccgtcaccg cgccgatctg
     1381 gctgtacgag gtatgcaccc tgcccgtgtt cgcatccaca gcggcgggca acgcatccac
     1441 atacgtagat ttcagcttcg agagttcgcg atgctcaagg atgaagtcca gcaccgggtg
     1501 ttgtccgcgc atcgagtcga gcacatcggc tgaggtggag taaaagcccg tggatgtttt
     1561 gcgtccctta tcgggtggtt cgaggcgcaa gtgattgaag agcacatccg aaagctgttg
     1621 tggcgagttg atgttgaagg tcttgccaac gtgaccgtaa atttcctttt cgatctcgtt
     1681 caaacgcgcc gccaattctt tagacatttc gccgaagaac ttggtatcga tcaatgcgcc
     1741 ggtcatttcc atttcagcca gcacagccgt cagcggcatg tcgatctcat ccaaaatctt
     1801 ttgcccttcc acgcgtttga gttctttctc ttcaattgcc atcaaacgca gggtcgtctc
     1861 cgcatcggca gccgcataat tggcaaccgc ttcaatcgcc acctctgcca tgctaatttg
     1921 ctttttgccc ttgccgatca gctcttcaat gtgggtcatc tcttcgccaa gccgatgctc
     1981 agagagattc ttcaagccca aatgccgaga ggcggggtcc acaatgaact ctgccaacat
     2041 ggtatcaaaa gtcaacggcg agacggtcaa cccatagcga gccagcacaa tgtaatcgta
     2101 cttggcatta tgcgccactt tcccgatctt cgcatttgtc atcgcagggg tgatcgcatc
     2161 gatcacggtt tgaagcggca ggttctcgcc agccacatgc ccaaccggga tgtaataacc
     2221 ttgcccttct tttacagcca gtgaaatgcc aaccaattca gccgtcattt cttcggtgcc
     2281 tgtcgtttcc gtatcgaacg aaatgacctt ggctttctcc aactcgttgg cgaggctctt
     2341 cagtttctcg gcgctatcca caatcacaac ttcaatgctc gccggtttga tcgccaccgc
     2401 cacgggttgc gcctcttctg cgaacatggt catctgcccg gctttggttt tgccggtttt
     2461 cacagcgggg gctttggtct ctgctggggc agaagccaca ccgccgctaa tggcaggcac
     2521 tttgcttacc agagttctga attccatttc tttgaagaaa gcttccaact tcgagccgtc
     2581 gaagggtgcc accttggcat gttcaaaatc gaatttgatc ttgaggttcg tttcaatctg
     2641 cgccaggtca cggctcatat acgccgtttc tttgttcgtc tcgaatttgc cacgccagcg
     2701 attttccact tggtctaagt tggcatagat cgcatccagc gt
//
LOCUS       DAOQFT010000345         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22892_length_2741_cov_6.759494, whole genome shotgun sequence.
ACCESSION   DAOQFT010000345 DAOQFT010000000
VERSION     DAOQFT010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22892_length_2741_cov_6.759494"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLR93_09100"
     CDS             complement(<1..812)
                     /locus_tag="PLR93_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH27163.1"
                     /translation="MKTKSTNSLSLFFNLTVLAAYFHALMEWLFFVTQSSSLSTLSLL
                     DKIEVLFSTGGVIVLLLLALLVVFTLPALKWKFFGYVPAALMVSVTALVMIDNFTYTV
                     FKFGIVSTSGAWRLLYTVAFVLLFYWVFRFVERQKLWKGVSFLSLGLLGVSTVVILSI
                     YNSRSPYMSDFTPEATEASAEHPNILILGSDGLSASYLSAYGFEGNTTPFIAELTQTS
                     LVAENAFPNVSSTTGSTTSALTGKMPAEVQVFRYPDILSKEDSFEHLPGILK"
     gene            complement(852..1466)
                     /locus_tag="PLR93_09105"
     CDS             complement(852..1466)
                     /locus_tag="PLR93_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH27164.1"
                     /translation="MRFFFNLLYHPFAFIYDLVAWVVSFGKWNDWVRSISPHLEGTRL
                     LELGHGPGHLQRFLLDLGLNPVALDESTQMGQIAKRRLGSKQKLTRGLAQNLPFAKAS
                     FETVVSTFPSEYIFDPKTLAEIKRVLVNEGKLIVLPAAFPSNGFLKWLYKVTGESPEA
                     LDDALKERLKSPFVSAGFNVEIQMIEMKSSTLVLILARSIFHNS"
     gene            complement(1536..1916)
                     /locus_tag="PLR93_09110"
     CDS             complement(1536..1916)
                     /locus_tag="PLR93_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNH27165.1"
                     /translation="MSDRDEFGAFLVGFVVGGLTGAVVALLFAPQSGEETRALIKDKS
                     IELRDKAQVSAEEAYARAEAAAKEARARAEELAKEARTRAEELTKEARTRAEQLASEV
                     KERGKGAIEAVRKPKKDGSEATPI"
     gene            complement(1952..2416)
                     /locus_tag="PLR93_09115"
     CDS             complement(1952..2416)
                     /locus_tag="PLR93_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27166.1"
                     /translation="MALPNPESTPQTPPPLTPEQLAELREQERKQKQIMAGIIAVVVL
                     LVALLGIAIYFLLQPTTPTDKIRDVFIIVVALETLVIGVALIVLVIQLASLINLLQNE
                     VRPILQATSETVNTLRGTAEFLGENFVEPVIKLNGYLAGLKRMLELLGVKPK"
     gene            complement(2539..>2741)
                     /locus_tag="PLR93_09120"
     CDS             complement(2539..>2741)
                     /locus_tag="PLR93_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27167.1"
                     /translation="KAMSVLVKPFDALMPDSYTSEGLRVISGVTYIGDNSKAKRELGY
                     NPRPVSEGWVETVKYEKQLSGR"
BASE COUNT          755 a          644 c          678 g          664 t
ORIGIN      
        1 tgctttagaa tgccgggcaa atgctcgaag gaatcttctt tggagaggat atcgggatag
       61 cgaaagacct gaacttctgc aggcattttc ccggtcaatg cagaggtggt ggagccggtg
      121 gtgctggaaa cgttagggaa ggcattttca gctacgaggg aggtttgggt cagttcggcg
      181 atgaagggtg tggtattgcc ctcaaacccg tatgcggata gatagctggc actcaaacca
      241 tcactgccca ggattaggat attggggtgt tctgccgagg cttctgtcgc ttcgggagta
      301 aagtcgctca tgtagggaga gcgcgaatta tatatggaga gaatgaccac tgttgaaacg
      361 ccgagcagcc ccaaagaaag gaaagaaaca cctttccaaa gcttttgtct ctccacaaag
      421 cggaagaccc aataaaagag cagcacaaag gcaactgtat acaacaagcg ccatgctcca
      481 gatgtggaga caatcccaaa tttaaaaacg gtataggtga agttatcgat catcaccaaa
      541 gcagtgacag ataccatcaa ggcggctgga acatacccga aaaacttcca cttaagagca
      601 ggcagagtaa aaacaaccag caacgccaat aaaagcagaa caatcacacc gccggtcgaa
      661 aacaagacct cgatcttgtc gagaagggaa agcgtggata gagacgaaga ctgcgtaaca
      721 aaaaacagcc attccataag ggcgtgaaag tatgcggcga ggacggtcag attgaaaaat
      781 agagataagg aatttgtaga ttttgttttc ataaagtcgg catattttac acccctgcat
      841 taaaaaaggc tttaggaatt atgaaaaata gaccttgcca gaattagtac caacgtgctc
      901 gatttcattt ctatcatttg tatttcaaca ttgaacccag cgctcacaaa cggacttttt
      961 agcctttctt tcaaggcgtc gtctaaggct tctgggcttt cgccagtgac cttatacaac
     1021 catttcagga atccattcga tgggaaggcg gcaggtagaa cgatcaattt cccttcattt
     1081 acaagaaccc gctttatttc tgccagtgtt ttggggtcga agatgtattc acttgggaag
     1141 gtggagacaa cggtttcaaa cgaagccttt gcaaacggca agttttgagc taaaccgcgc
     1201 gtcagttttt gcttggagcc gagacggcgt ttggcgattt gccccatctg tgtagattca
     1261 tctagggcaa caggattcag ccccaggtcc agtaagaagc gctgcaagtg accggggcca
     1321 tgccccaact caagaaggcg agtcccttca aggtgtgggg aaatgctacg aacccagtca
     1381 ttccacttcc caaacgaaac gacccacgcc accaggtcat atatgaaagc gaagggatgg
     1441 taaagcaggt taaagaaaaa acgcatgaag tgacgcatgg cagaaataaa acgagggttt
     1501 gacctgagtc aaaccctcat ctgtttcaat aaagattaaa taggggtggc ttcgctgcca
     1561 tctttcttcg gcttgcgaac ggcttcgatg gcgcccttgc cgcgttcttt tacttcagaa
     1621 gcgagctgtt ctgcacgagt gcgagcttct ttggtaagtt cttcggcgcg ggtgcgagct
     1681 tctttggcaa gttcttcagc gcgagcgcgg gcttctttgg cagcggcttc tgcgcgggca
     1741 taggcttctt cagcgctcac ttgggcttta tcgcgcaatt caatcgattt gtccttgatc
     1801 aacgcacggg tttcttcgcc agattgagga gcaaagagca aagcaacaac agcgccggtg
     1861 agcccgccga caacaaaacc tacgagaaag gctccaaatt catcacggtc agacatggta
     1921 gttctccttt ttagtcagat gatgtcatat actatttagg ttttacacct aacaattcta
     1981 acatacgctt taagcctgcc aaatagccat taagtttgat cactggctca acaaaatttt
     2041 ctcccaaaaa ttctgcggtg ccgcgcaggg tgttcacggt ttcgctggtt gcctgcaaaa
     2101 tggggcgcac ttcattctga agcaggttga tgaggctcgc caattggatg acaagcacga
     2161 tcaacgccac accaatcacc aaggtttcaa gcgccacgac aataatgaac acatcgcgga
     2221 tcttgtcggt aggggtggtg ggctgcaaca ggaaataaat tgcaatacca agcaaggcaa
     2281 ccaacaaaac gaccacggca ataatgcccg ccatgatctg tttttgcttg cgttcctgtt
     2341 cgcgcagttc agccaattgt tcaggagtta acggaggggg agtttgtgga gtggattcag
     2401 gattgggcag tgccatgtct gtattatagc agattagata tacgaaaaaa agccagagga
     2461 tgaaacaagt cgcctctttc ccgtgccgcg cttgtaccta accatctggc ttttttcttc
     2521 tgccaaactt ggttctgcct agcgtcccga taattgtttt tcgtacttaa ctgtttctac
     2581 ccaaccctca ctcaccgggc gcggattgta gcccagttcg cgtttggctt tgctgttatc
     2641 accaatgtac gtcacgcccg atatgactcg caacccttcc gatgtgtatg aatcgggcat
     2701 cagcgcatca aaaggcttga ccaaaaccga cattgctttc a
//
LOCUS       DAOQFT010000346         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_22938_length_2739_cov_5.648286, whole genome shotgun sequence.
ACCESSION   DAOQFT010000346 DAOQFT010000000
VERSION     DAOQFT010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22938_length_2739_cov_5.648286"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1116
                     /locus_tag="PLR93_09125"
     CDS             94..1116
                     /locus_tag="PLR93_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27168.1"
                     /translation="MRVTKDSLLRIAKETVQERAYNDPDIVAAYLTGSLRGEPMLGGT
                     ADIDIVFVHKTTPMKVREFVKLTPDFHIDISRRAKDEFKSPRELRGDPWLGYEMYDPV
                     LLYEREKFFDFVQASLRAGFEFEQPPLMLQRCRTLLNHGRGNWMDISEHDGEVGPKEI
                     RKYMKSLFHAVNAVAELSGPPIWERRLLLDFPARAEAIQKPEMITTVYGLLGATQVDA
                     EKIKTWLNDWKHAFKLASETPGVDARIHPARLNYYEKSIKALLDSDTPLSALWPVLHT
                     WTLSAKVLSGDHLKFWEQACNSLGLLEGFADKVSGLDHFLDEIEIIFEEIATANGLDV
                     EPSTGL"
     gene            1265..1609
                     /locus_tag="PLR93_09130"
     CDS             1265..1609
                     /locus_tag="PLR93_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YqfO family protein"
                     /protein_id="HNH27169.1"
                     /translation="MYDFTDVKLEIFVPETHVNYVRDALVEVGIGVIGNYDHCFAISQ
                     VQGSFRPLPGSNPFDGKIGEVTYSVESKIEVNCRRELVEEALAAVRRSHPYEEPLINI
                     FPLANHLFTRNV"
     gene            1606..2649
                     /locus_tag="PLR93_09135"
     CDS             1606..2649
                     /locus_tag="PLR93_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH27170.1"
                     /translation="MKSKNLVYIFQALLAALFFGASAPIAKLLLGDIAPIYLASFLYL
                     GSGTGIALTKLTQPKGTEASIQRADVPWLAGAIISGGILAPIVLMNSLQNTPASTASL
                     LLNFEGVGTTLLAALFFKESISRRAWMAIIVITLASIFLSTDFSGGFELSLGALGIIL
                     ACVLWGADNNFTRNISGKDPLVIVAWKGLVAGSFSFILASSLGNPFPAVTTILGTLLL
                     GFLSYGLSTMLFIRSMRGLGAARTSALYGTAPLAGVALSIFMFGEFPSFFFIIAAILM
                     IGGALLLINEEHSHTHVHTAIVHEHSHSHDDGMHGHSGGEGIHSHGHEHPEEEHDHDH
                     MPDIHHRHGHEDK"
BASE COUNT          646 a          644 c          669 g          780 t
ORIGIN      
        1 aatgcggagc atatcaagtt gatcgaaaat cgcatccgtg aagctttggg gctgtcacct
       61 tatcagcccg ccttgttgaa ataggcagga cccatgcgcg ttacaaaaga ctccctttta
      121 cgtattgcca aagaaaccgt tcaagaacgg gcgtataacg atcccgatat tgttgcggca
      181 tatctcaccg gctcgttgcg cggcgaaccc atgcttggcg gcactgccga cattgacatt
      241 gtcttcgtcc acaaaaccac accgatgaag gttcgtgaat tcgtaaaact cacgcccgat
      301 tttcatattg atatatcccg ccgtgcaaag gatgaattca aatctccgcg tgagttgcgc
      361 ggtgatccgt ggctcgggta tgaaatgtat gacccggttc tgttgtatga acgtgaaaag
      421 tttttcgact ttgtgcaagc cagtctgcgt gctggcttcg agtttgagca gccgccgttg
      481 atgttgcagc ggtgccgcac acttctcaac cacgggcgcg gcaactggat ggatatttcg
      541 gaacacgatg gggaagtggg tccgaaagaa attcgcaaat acatgaagtc gctttttcat
      601 gcggtcaatg cggttgcaga gttgagcggt ccgcccattt gggagcgccg tctcctgctt
      661 gattttcccg ctcgagctga agccattcaa aagcctgaaa tgatcactac cgtatatggt
      721 ttgcttggtg ccacacaagt ggatgcagag aagattaaaa cctggctcaa cgattggaag
      781 cacgccttca aactcgcatc tgaaactccc ggcgtggatg caaggattca ccccgcacgc
      841 ctgaactatt acgagaaaag catcaaagcc ctgctggata gtgatacgcc tctttccgcg
      901 ctctggcctg ttctgcatac ctggacctta tctgccaagg tgctgagtgg tgaccatctc
      961 aagttctggg aacaagcttg caactcgctt gggctacttg aaggctttgc agacaaagtc
     1021 tctgggcttg atcacttcct tgatgagatc gagattattt ttgaagagat cgctaccgcc
     1081 aacggattgg atgtagaacc atcgacaggt ttataaatgc gagtataatc gcgccgatgt
     1141 ttatagggta tccgcaccgc catttattag acaagtcccg cccgggcgca ttctttgttt
     1201 tctttcatcg ttctcaaaag cctgacaatt aaatgtcagg cttttttctt aatggagata
     1261 ttcgatgtac gactttactg atgtcaaact cgagattttc gtccctgaaa cgcatgtcaa
     1321 ctatgttcgc gatgcgctgg tagaagttgg gattggtgtg atcgggaact atgaccactg
     1381 ttttgccatc tcacaagtgc agggctcgtt ccgcccattg ccggggtcga atccgtttga
     1441 tgggaaaata ggggaggtca cttacagcgt agagtcaaag atcgaagtga attgcaggcg
     1501 tgagcttgtg gaagaggcgc ttgccgcagt gcggaggtct catccttatg aagaaccact
     1561 tattaatatc tttccgttgg cgaatcatct atttacgagg aatgtatgaa gtcaaagaat
     1621 cttgtttata tttttcaagc tctgctcgcc gctttgttct ttggtgcaag tgcaccgatc
     1681 gcgaaattat tgctgggtga tattgcgcca atttaccttg cttccttttt gtatctcggc
     1741 agcggaaccg gaatcgccct cacaaaactt actcaaccca aaggaaccga agccagcatt
     1801 cagcgtgcag atgtgccttg gctggcgggt gcgattatct ctggcggtat tcttgcacca
     1861 atcgtcctta tgaatagttt gcaaaatacg cctgcatcaa cggcttcgtt actgcttaat
     1921 ttcgaaggtg tagggactac tttgttggct gctttatttt tcaaagagtc gattagtcgc
     1981 cgcgcatgga tggcgatcat tgttattacc cttgcaagta tttttctttc tactgatttt
     2041 tcaggcggtt tcgaactttc acttggcgct ttgggtatca tccttgcttg tgtactttgg
     2101 ggcgcagaca ataatttcac gcgcaatatt tctggtaaag acccgctcgt catcgtagca
     2161 tggaagggat tggttgctgg ttcattttct ttcatcttag cttcgtcgct tggaaatcca
     2221 ttccctgcgg tgacgactat tcttggaacg ctgctgttgg ggtttcttag ctatggattg
     2281 agcacaatgc tgttcatccg ctccatgcgc ggacttggcg ccgcccgaac cagtgctttg
     2341 tatggtacgg ctccgctggc gggtgttgct ttatctattt ttatgtttgg tgaatttcct
     2401 tctttctttt ttattatcgc cgcgattttg atgatcggcg gcgcgttgct tctgattaat
     2461 gaagagcatt ctcataccca tgttcataca gcaattgttc atgaacatag ccatagccat
     2521 gatgatggta tgcacgggca tagtggcggc gaaggtatcc attcgcatgg gcatgaacac
     2581 cctgaagaag aacatgacca tgaccatatg ccagatattc accatcggca tgggcatgaa
     2641 gacaagtaaa taccgatata tgcccaactc atatatcgaa ttgataacca attatcagaa
     2701 ccaggagact aagaaatgcc aacaacttcc acgaacccc
//
LOCUS       DAOQFT010000347         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_23227_length_2720_cov_4.976735, whole genome shotgun sequence.
ACCESSION   DAOQFT010000347 DAOQFT010000000
VERSION     DAOQFT010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23227_length_2720_cov_4.976735"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLR93_09140"
     CDS             <1..704
                     /locus_tag="PLR93_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27171.1"
                     /translation="FLSSQSYNGIYYELQGDNLEPLQNLVNRPVKIWGDISYSDTGTM
                     FLDVKQFEEIYPGLQFQILQGKEALTEINGKPVALFTTNGTTYVMLSEVGAYQDISYK
                     GDFPEDINIEALIVPGETYEGMPALRVFSMANAKDPNTGEAIELEPFAGEIQSSPDPF
                     GNSDVYVEPDFIVDYIQLEYYVTDPKMLYDAPTTDTLYIQPVWHFSGHYTNGTVSDIL
                     VQALKQEYLYPFNSP"
     gene            779..1381
                     /locus_tag="PLR93_09145"
     CDS             779..1381
                     /locus_tag="PLR93_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNH27172.1"
                     /translation="MDQLLLILTYIAVIASSVSGALGARKYQMDIVGAVTIAYVNAFG
                     GGTVRDLLLGRTPIFWIADPALTVTTFIVAIISFYRLDRISKLLLTTADAIGLGIFSI
                     LGATYTLQLNLSPIVAIHMGVVTGIFGGVLRDVLSNRIPKVFGQSTELYATCSFAGTT
                     VFIILYVLHVNTLLASLAGALTIFTMRLLAVRFKMTLPSP"
     gene            1415..2155
                     /locus_tag="PLR93_09150"
     CDS             1415..2155
                     /locus_tag="PLR93_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNH27173.1"
                     /translation="MISTYHRPQTLDEALTLLTQPNTVPLGGGTLLSKATADSVAVVD
                     LQRLGLDSLRVNGNELQIGATCTLQSLLESANCPASLKTAIKLEAPLNIRNTATIAGT
                     LVASDGRSPFTAMLLAMDAKLDYRVLTSQSSIGLSEFLLTRPSGLVTSITIPLNTKTA
                     FEFVSKTPADKPIVGAAVAQWNSGRTRLTLCGYSKTPMLAMDGTEADGIQEAARNAYH
                     EANDEWASAEYRMDVAAVLAGRALAALT"
     gene            2159..2425
                     /locus_tag="PLR93_09155"
     CDS             2159..2425
                     /locus_tag="PLR93_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27174.1"
                     /translation="MQTCPICNKEVQPSQRYPNYVCAECADLAVSKDGRKLIFYNQSI
                     SGGYEAKYADTGEEYNSHDCYINGIKCHANEHHFGGIVIIKVES"
     gene            2425..2700
                     /locus_tag="PLR93_09160"
     CDS             2425..2700
                     /locus_tag="PLR93_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27175.1"
                     /translation="MTKEKIKAFEEIARIFEISNESAKYFLNHVQKSFKVERPPHKLI
                     LEFVEKQNYEFKPTPYDVALAMKESAVWDYDLGSPPPALEDEEDLYQ"
BASE COUNT          745 a          741 c          556 g          678 t
ORIGIN      
        1 ccttcttaag ctcacagtcg tacaatggaa tctattacga attgcaaggc gataatcttg
       61 agccacttca aaacttggtt aaccgcccgg ttaagatttg gggcgatatc agctactcag
      121 atacagggac gatgttcttg gacgtaaaac agttcgaaga aatctacccg gggttgcaat
      181 tccagatact gcaaggcaaa gaagcgctta ccgagatcaa tggaaaaccc gttgcgctct
      241 ttacaaccaa tggcaccacc tatgtgatgc tctcagaagt aggtgcttat caagatatta
      301 gttataaggg tgatttccct gaagatatca acattgaagc tctgatcgtg ccgggtgaaa
      361 catacgaagg catgcccgcc ttgagggtat tcagtatggc aaatgccaaa gacccgaaca
      421 ccggagaggc tattgaatta gaaccatttg ctggcgagat acaatcatct ccagatccat
      481 tcgggaactc agatgtatat gtcgaacctg atttcatcgt tgattacatc caactcgaat
      541 actatgttac cgaccccaag atgctttacg acgctcccac cacagacacg ttgtacattc
      601 aacccgtctg gcactttagc ggacattaca caaacggcac cgtctctgat attctcgtcc
      661 aagcgctcaa acaagaatat ctctacccct tcaattcacc gtaaccagta aagcaacaaa
      721 aaagtccacc atcacatgat ggtggacttt ttaatacaag ttgtgtgata cttggcgaat
      781 ggatcaactt cttctcattc tcacttacat cgccgtcatc gcatcatcgg tttcaggagc
      841 gctaggcgca cgtaaatatc aaatggacat tgtaggcgca gtgaccattg cctatgtcaa
      901 cgcctttggc ggcggtaccg tccgcgactt attgctgggg cgcacgccca tcttctggat
      961 cgccgaccct gcattaaccg tcactacgtt catcgtcgct attatctcct tctatcggct
     1021 cgaccgtatc tcaaaactac tgctcaccac agccgatgcc attggtctcg gcatctttag
     1081 cattcttggc gcaacctata ccctacaact caatctttct cccatcgttg ccattcacat
     1141 gggcgtggtc actggcatct ttggcggcgt cttacgtgac gtgttgagca accgcatccc
     1201 caaagtgttc ggtcagagta ccgagcttta tgccacttgc tctttcgctg gcacaacagt
     1261 atttatcatt ctttatgttc tgcatgtgaa caccttgctt gcatcgctag caggtgcgct
     1321 caccattttc accatgcgtc tactcgcagt ccgcttcaaa atgaccctcc cctctccata
     1381 acgatttata atgcatatct atggaggcac ttctatgatc tcgacctatc acagacctca
     1441 aactctcgac gaagccctga cgcttctgac tcaacccaat accgttcccc tcggaggcgg
     1501 tacgcttctt tccaaggcga cagctgactc tgttgccgta gtcgatctcc aacgcctcgg
     1561 cttggattcg ctacgcgtca acgggaatga actccaaatc ggtgcaacct gtaccctaca
     1621 gtctctgctg gaatcagcta attgccccgc atcgctaaaa accgcaatca aactcgaagc
     1681 tccgctcaat attcgcaaca cagcgacaat tgccggaact ctcgtcgcca gtgacggacg
     1741 ttctcctttc acagcaatgt tactagccat ggatgcaaaa ctggactata gagtattaac
     1801 cagtcaatca tctatcggtc taagtgaatt cctcctcacc cgtccaagcg gactcgtcac
     1861 ctccattacc attccgctca ataccaaaac cgctttcgaa ttcgtttcca aaaccccggc
     1921 agacaaaccc atcgttggag ctgccgtcgc acaatggaat tcagggcgaa cccgccttac
     1981 cctgtgtgga tacagcaaaa ctcccatgct cgccatggat ggtaccgaag cagatggcat
     2041 tcaagaagcc gcacgcaacg cctatcacga agccaacgac gaatgggctt ctgccgaata
     2101 ccgtatggat gttgcggcag tgctggcagg gcgtgcattg gcagcactta cctagtctat
     2161 gcaaacctgt ccaatctgca acaaagaagt acaaccttcg caacgttacc ccaattacgt
     2221 ctgcgccgag tgtgctgacc tcgcagtatc aaaagatggc agaaaactaa tcttctacaa
     2281 ccagtcaatt tctggtgggt atgaagccaa atacgctgat accggcgaag aatataacag
     2341 ccacgattgt tatattaatg ggattaagtg tcacgccaac gaacatcact ttggcggcat
     2401 tgtgatcata aaagtagaaa gctaatgacc aaagaaaaaa ttaaagcctt tgaagaaatc
     2461 gcgcgtattt ttgagatatc caacgaaagt gcaaagtatt ttctcaacca tgttcagaaa
     2521 agctttaaag ttgaacgccc gccccataaa ttgatccttg aatttgttga aaaacaaaat
     2581 tatgaattca agccaactcc atacgatgtt gccttagcca tgaaagaaag cgcggtttgg
     2641 gattacgacc tcggttctcc gccacctgcc ttggaagacg aagaagatct ctatcaataa
     2701 aagcaactag catcggtgat
//
LOCUS       DAOQFT010000348         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_23297_length_2715_cov_5.942105, whole genome shotgun sequence.
ACCESSION   DAOQFT010000348 DAOQFT010000000
VERSION     DAOQFT010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23297_length_2715_cov_5.942105"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="PLR93_09165"
     CDS             <1..1592
                     /locus_tag="PLR93_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH27176.1"
                     /translation="AGGDATQAPAAGENSGGTKTATFIFTQEFDNLNPLYTNQWFTAI
                     TYQLWSCYAWTYDDQNAPVPNLVTELPTTENGGISEDGRTLTFKLRDDIVWSDGEPIT
                     ADDFVFTYEMTMDPANTVASQSPYDQMESVVAADPQTVVVTFTEPFAPWAATLWKGII
                     PKHVLQPVFEADGNLNAAEYNRAPTVGCGPFNFAEWEAGSFARFVANDNYWLGRPKLD
                     EIFIRFVPDDASQIAALQAGEGDLGTFIAYSDIPALEEAGVQMFTVFSGYNEGLYLNL
                     GEKGHPALKELGVRQAIAYALDRESLVNDLLLGRTGVAATYWDNTPFVDPSVAPYPYD
                     PAKANELLDAAGWTDSNGDGTRDKDGVELVLKYGTTTREIRKEIQAVFQQQLLEVGIG
                     TELINAENFFDGYAEGGPAATGSLDIFEYSTTANFPDPHTSEWFCDQIPTDAAPDGSN
                     WQAVCDTDLEALFTTESSQVDAAARETTFHKISKHVFDQAYWIGIYWDPDIWAVNARL
                     TNVKLSGATPFYNIMEWDLAE"
     gene            1668..2702
                     /locus_tag="PLR93_09170"
     CDS             1668..2702
                     /locus_tag="PLR93_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27177.1"
                     /translation="MTKYIVRRLLQAIPLLLLISLILFVLMQNATDPLATMGGRTPTK
                     PEDRARLTRQLGLDKPIHIQYLYWLAGNDWAKTDLDGDGIAETYGTRKGVLRGDFGTS
                     IVQTGRPVTEVIWERLPNTLMLMLTSEIIIVLGALLVGVYSALRQYSFFDNLLTGVLF
                     VGYSMPIFFIALSTMYIFSVNFKRWGLPYLPTVGMFDPKVGETPGQIILHMILPVFSI
                     SFISFAAYSRYIRSTLLEVLGQDYIRTARSKGLPKREITFIHALKNASLPIVTVVGLD
                     LPLLLGGAVVTERIFAWPGMGRLFIDGIDRGDTPVVMGILMLIAIAVVVFQILTDIVY
                     AWLDPRIRFD"
BASE COUNT          613 a          685 c          656 g          761 t
ORIGIN      
        1 atgccggtgg cgatgcaact caggcacctg ctgccggtga aaatagcggt ggaacgaaga
       61 ccgctacttt catttttacg caggagttcg ataacctgaa cccgctctac accaatcagt
      121 ggttcactgc cattacatat cagctctgga gctgctatgc atggacctat gatgatcaaa
      181 acgcccctgt tccgaatttg gtgactgaac tgcctaccac cgaaaatggc ggtatctctg
      241 aagatggtcg taccctgact ttcaagttgc gcgatgatat cgtatggtct gatggtgagc
      301 ctatcactgc tgatgatttc gtattcacct acgaaatgac catggaccct gcaaataccg
      361 tggcttctca gagtccctat gaccagatgg aaagcgttgt cgctgcagac ccgcaaactg
      421 tggtggtgac tttcaccgaa ccgtttgcgc cttgggctgc aaccctttgg aaaggcatca
      481 ttccaaagca tgttcttcag cctgtgtttg aagcagatgg caacttgaat gccgctgaat
      541 acaaccgcgc accgactgtc ggttgtggtc cattcaattt tgctgaatgg gaagctggct
      601 cttttgcccg ttttgttgcc aatgataact attggctcgg acgccccaag ttggatgaaa
      661 tcttcattcg cttcgtaccc gatgatgctt ctcagatcgc tgctcttcag gctggtgaag
      721 gtgatctcgg tacgttcatt gcctactcag acattcccgc ccttgaagaa gcaggcgtgc
      781 aaatgttcac cgtcttctct ggctataacg aaggtttgta cctgaacctt ggcgagaaag
      841 gtcaccctgc gctgaaggaa ttgggtgttc gccaggcaat tgcttatgca cttgaccgcg
      901 aatcccttgt caatgattta ctgttgggtc gcactggcgt ggctgcaaca tactgggata
      961 acactccgtt cgttgacccg tctgttgctc cctatcctta tgaccctgcc aaggcaaacg
     1021 aacttctcga tgccgctggc tggaccgact ctaatggtga cggcacccgc gataaggacg
     1081 gtgttgaact cgtcttgaag tatggaacca ctacccgtga aatccgcaag gaaattcagg
     1141 ctgtgttcca acagcagtta cttgaagtag gcattggcac cgaattgatc aacgccgaaa
     1201 acttcttcga tggctatgct gaaggtggtc cggctgcgac cggctcgttg gatatcttcg
     1261 aatattccac cactgccaac ttcccagatc ctcatacttc tgaatggttc tgtgaccaga
     1321 tcccaaccga tgccgccccc gatggcagta actggcaggc tgtctgcgat actgacctcg
     1381 aagctctctt cactactgaa agcagccagg ttgatgctgc cgctcgtgaa acaaccttcc
     1441 ataagatttc gaagcatgta ttcgatcagg cttactggat cggtatttac tgggatcctg
     1501 atatttgggc agtcaatgct cgccttacca atgtcaagct ttctggcgcc actccgttct
     1561 ataacattat ggaatgggac cttgctgaat aagattttga gttagtacta aattagcagg
     1621 ggttctgtaa taaaattgca gaacccctgc tttttgaagg tgaatccatg accaagtaca
     1681 ttgttcgccg tctgttgcaa gccattccac tcttactgct tatcagtctg atcctcttcg
     1741 ttctgatgca gaatgcgacc gacccattag caacgatggg tgggcgtaca cccaccaagc
     1801 ctgaggatcg cgcccgcctc acccgccaat tggggctgga taaacccatc cacattcaat
     1861 acctctattg gctggctggc aatgattggg caaaaaccga tctggacggc gacggaattg
     1921 ccgaaactta tggaacgcgc aaaggtgtac tgcgtggaga ttttggaacg tccatcgttc
     1981 aaacgggacg ccctgtcacc gaggtgattt gggaacgctt accaaacacc ttgatgctga
     2041 tgcttacatc agaaattatt attgttcttg gggcattgtt agtgggcgta tattctgctt
     2101 tacgccaata ttcattcttt gataatcttc tgacgggggt gctctttgtt gggtattcca
     2161 tgccgatctt tttcatcgca ttatctacga tgtacatttt ttcagtcaac ttcaaacgct
     2221 gggggctccc atatcttcca actgtaggga tgttcgaccc taaagttggc gaaacgccgg
     2281 ggcagatcat tttgcacatg atcctgccag tctttagcat ttcatttatt agctttgctg
     2341 cttacagccg ttacatccgc agcactctgc tggaagtact tgggcaagac tatatccgca
     2401 cagcgcgctc aaaagggctg cccaagcgag agatcacgtt tattcatgct ctcaaaaacg
     2461 cctctcttcc gattgtgacc gttgttgggt tggacttgcc actattacta ggcggtgctg
     2521 tggtgacaga gcgtatcttt gcctggcccg gtatgggcag gctttttatt gatggcattg
     2581 accgtggaga tactcctgtc gttatgggca ttctcatgtt gattgctatt gctgttgttg
     2641 tttttcaaat cctcacagat attgtatacg cctggcttga cccgcgtatt cgctttgatt
     2701 aggagagagt tatgt
//
LOCUS       DAOQFT010000349         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_23468_length_2702_cov_6.076691, whole genome shotgun sequence.
ACCESSION   DAOQFT010000349 DAOQFT010000000
VERSION     DAOQFT010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23468_length_2702_cov_6.076691"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLR93_09175"
     CDS             complement(<1..849)
                     /locus_tag="PLR93_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH27178.1"
                     /translation="MNSRSQSFFVYFLLLVAIGAMIFVGLRDNSSAAEPLTISEVARM
                     IQGGQVARIIIESDDTIRVVKVNGSEETALESRKESNTTLIEQLRDYGVTPEQLAEMN
                     IEVSAPSPWAGVLSGALYLLPVLFMGGLLWFIFRQAQGSNNAAMSFGKSRARMFSGEH
                     PTVTFADVAGADEAKQELAEIVEFLKEPQKFIQLGARIPKGVLLVGPPGTGKTLLSKA
                     VSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIIFVDEIDAVGRQRGA
                     GLGGSHD"
     gene            complement(1049..1813)
                     /locus_tag="PLR93_09180"
     CDS             complement(1049..1813)
                     /locus_tag="PLR93_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNH27179.1"
                     /translation="MNIITWNVNGIRAALGKNALDWAFEKKPDALCLQEVKARPEQLN
                     EEQAAQLKLPYIWNPAERPGYSGVATFYKTHPDETALGMDAPEFDVEGRIIQTRWGNV
                     RLFNIYFPNGQRGHDRVDYKLRFYARLLETCNTLHSKGELVVITGDFNTSHMPIDLKN
                     PKANEKTSGFMPEEREWVQKYLDNGFADAYRRLYPDRVQYTWWTYRLDARARGIGWRL
                     DYFLVSEALMPRVKDVIIHEQVMGSDHCPVELVLNV"
     gene            1865..2251
                     /locus_tag="PLR93_09185"
     CDS             1865..2251
                     /locus_tag="PLR93_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH27180.1"
                     /translation="MDETPTKTILCVEDEPEMIDLIRLILSRKGYSVKGASGGVEGIR
                     LIKELHPDLVLLDLMMPDMDGWEVYQQMKAEASLREIPVIVVTAKAQNIDKVLGLHIA
                     KVDDYIAKPFSPQELVDSVDKVFSHK"
     gene            2304..2675
                     /locus_tag="PLR93_09190"
     CDS             2304..2675
                     /locus_tag="PLR93_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNH27181.1"
                     /translation="MPRPITIQNLSRTLEKPATVGYCDSFQYKLRGLMFRSHLDINDG
                     LLLVEKRNSRLDTSIHMFFVPFDLTVVWINSDLTVVDKVLAKSWKPAYFPKADAQYTL
                     EIHPDRWADYEIGDKVEFKNA"
BASE COUNT          724 a          677 c          615 g          686 t
ORIGIN      
        1 atcgtgtgaa ccgccgagtc ctgctccacg ttggcgaccc accgcatcaa tttcatctac
       61 aaagataata caaggcgaat ggcgcttggc ttggtcgaac aggtcacgca cacggctggc
      121 gcccacacct acgaacattt ctacgaattc agaaccagag atagagaaga agggcacgcc
      181 tgcttcacca gaaacagctt tagaaagaag ggttttaccg gttccgggag ggcccaccaa
      241 caaaacaccc ttcggaatac gagcaccaag ttggataaac ttctgcggct ctttcaggaa
      301 ctcgacgatc tctgccaatt cctgtttggc ttcatcggcg ccggcaacgt cggcaaaggt
      361 gacggtggga tgttccccac tgaacatgcg cgcacgagat ttaccaaagg acatcgctgc
      421 attgttgctg ccttgtgcct ggcggaaaat gaaccaaagc aacccaccca taaacagaac
      481 cggtaacaga tacaaagcgc cgctcaacac acccgcccat ggcgatggtg cgctgacttc
      541 gatattcatt tcagcaagtt gctcgggggt tacaccataa tcacggagtt gttcgataag
      601 ggtcgtattt gactctttgc gggattcaag ggctgtttct tcagacccat tgacctttac
      661 cacacgaatg gtatcgtcgc tctcaatgat gatgcgtgcc acttgcccgc cctgaatcat
      721 ccgtgccacc tcactgatgg tgagcggctc ggcagcgctg gaattgtctc gcaagccaac
      781 aaaaatcatc gctccgatgg ctacgagcaa taaaaagtac acaaaaaacg attgactacg
      841 ggaattcacg gaatcctcca aatttgacgt acgcggtcat tataccaagc cataccagta
      901 gagataccgt ctgggctttt attattacga ttttctaaca cggttcaaag tctaaccgga
      961 acctgcttgc aaaaccaatc aatcttttgt caccctaggg gataatttca acggaactta
     1021 ttagaaatta atcagccatc cacaaagttt acacatttaa aaccagctct accggacaat
     1081 ggtcagaacc catcacctgc tcatgaatga tgacatcttt tacgcggggc atgagcgcct
     1141 ctgagacgag gaaataatcc aagcgccagc caattccgcg ggcacgagca tcgaggcgat
     1201 aggtccacca ggtgtattgg acccggtctg ggtagaggcg gcgataggca tctgcaaagc
     1261 cgttgtctaa atatttttga acccactcgc gttcctctgg cataaagcca gaggtcttct
     1321 cgttggcttt gggattcttc aagtcgatgg gcatgtgaga ggtgttgaag tcgcctgtaa
     1381 tgacgaccag ctctccctta gaatgaagcg tgttacatgt ttcaagcaaa cgggcataaa
     1441 agcgtaattt atagtccacg cggtcatgtc cgcgttgtcc atttgggaaa taaatattga
     1501 agagacgtac gttcccccaa cgggtttgga tgatgcgccc ttctacatca aattcaggcg
     1561 cgtccattcc aagtgctgtt tcatctggat gtgtcttata aaaagtcgct accccactat
     1621 accctgggcg ttcggcagga ttccagatgt aaggcagctt caattgagcg gcttgttctt
     1681 catttagttg ttcagggcgg gctttaactt cctgcaaaca caacgcatct ggcttttttt
     1741 cgaacgccca atcaagagca ttcttgccaa gtgcagcgcg aattccgttc acattccagg
     1801 taatgatatt catacgaagt tggttggtct ctttatggta aacttatttt agaaggcaaa
     1861 aattatggat gaaacaccta ccaaaaccat cctatgcgtt gaagatgaac ccgaaatgat
     1921 tgatctgatt cggttgattc tttctcgaaa ggggtatagc gtaaaaggcg cgtctggcgg
     1981 tgtggaagga attcgcctta ttaaagaact tcatccagac cttgttttgc ttgatcttat
     2041 gatgcccgat atggacggtt gggaagtata ccaacaaatg aaagccgaag cttctctgcg
     2101 cgaaatcccc gttattgttg tcactgccaa agcacaaaat atcgataaag ttcttggttt
     2161 acacatcgcc aaagtggatg actacattgc caagccgttt agtccacaag agttagtgga
     2221 tagcgtcgat aaagttttta gccataaata attttcattc caaaacgggc ggcgaatacg
     2281 ccgcccattt ttcataaaac tccatgcctc gccccatcac catacaaaac cttagtcgaa
     2341 ccttggaaaa gcccgccact gtgggctatt gtgactcgtt tcagtacaaa ctgcgcggat
     2401 tgatgttccg ctcgcatctg gacattaacg atggcttgct cctcgttgaa aaacgaaact
     2461 cgcggctcga cacttccatt catatgttct ttgtgccatt tgaccttacc gtcgtctgga
     2521 tcaactccga cctgacggtg gtggataagg ttcttgcaaa atcatggaag cccgcctact
     2581 tccccaaggc agatgcacaa tacacgttgg aaatacaccc agaccgttgg gcggattatg
     2641 agattggcga taaagttgaa ttcaaaaatg cgtaaacgtt ttttcatagc tcttctgctc
     2701 ct
//
LOCUS       DAOQFT010000350         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_23553_length_2696_cov_7.350246, whole genome shotgun sequence.
ACCESSION   DAOQFT010000350 DAOQFT010000000
VERSION     DAOQFT010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23553_length_2696_cov_7.350246"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PLR93_09195"
     CDS             <1..1584
                     /locus_tag="PLR93_09195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH27182.1"
                     /translation="GVLYNLSMLPPNVLYSAILLAAGVGCLVVAVAIWLKRREAIGAK
                     SLFVFLLGLSWWDITYGIFWADIPGPNPYFWLDITLFGAFVVPTAFLIFALEYSNSQK
                     WLGRPFIIAAIIEPILISILLCTDRWHNLYFGGVRALNTTMLVEMGPAAWANVVYSYL
                     LILIGVLVLAVTLYRSRGVYRMQVTFILAATVTPWVIHILFLSIGDHALLPDADVTPF
                     IFSMTALLIAYALIRYRLLDVVPIARSILIESMSEGVCVLDSQNRIVDINQTARKALS
                     SEFFIGQPVEQAFKCWIDIVSHFFDQETAQTEVVIDDRYLDLRISPVYDKNQRLLGKL
                     VVWRDITELKNTQLKLQQLATIDELTLAFNRRHFMEIAEVQFSQALRQNQPFALALID
                     LDYFKGINDKYGHPAGDQVLAEFSRMFMENIRKFDVFGRLGGEEFALLMPNTDIHFAY
                     HASERLRFMITNTPIPINGGDVIVTFSLGVAMLQGAQDTLSAIFRRADLALYAAKNSG
                     RNQVKVWEQGLEAVAPKQA"
     misc_binding    1631..1881
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1951..>2696
                     /locus_tag="PLR93_09200"
     CDS             1951..>2696
                     /locus_tag="PLR93_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNH27183.1"
                     /translation="MFKAVNPKLDVPSMEDNVLKMWTKQEIFKKTVDQRQGKPEYVFY
                     EGPPTANGRPGVHHVLARAFKDMFPRYKIMRGYHVSRRGGWDTHGLPVEIEVEKQLGF
                     TNKQQIEEYGVDKFNELCKKSVFNYIQEWEKLTGRIGFWVDLDTAYVTYTNDYIESVW
                     WILKNFWEKDLLFKGYKIVPYCPRCGTPLSDHEVALGYEDTADPSVFVRFPLVDKPDT
                     SFLVWTTTPWTLPANVAIAAHPDVDYVTVER"
BASE COUNT          643 a          584 c          695 g          774 t
ORIGIN      
        1 ggggtattgt ataatttgag tatgcttcca ccgaatgtat tgtatagtgc aattttgttg
       61 gcggcaggtg tggggtgttt ggtagttgcc gttgcaattt ggctgaaacg ccgtgaagcc
      121 ataggggcga aatcgctttt tgtattccta ttagggctaa gctggtggga tataacctat
      181 ggcatctttt gggcagatat acctggacca aacccatatt tttggttaga tatcaccttg
      241 tttggtgctt ttgttgtgcc gaccgcgttt ttgatttttg cattggaata ctctaattcg
      301 caaaagtggc tggggcgacc ttttattatt gcagcgatca tcgaacccat cttgatttcc
      361 atcctgttgt gtacagaccg ttggcataac ctttattttg gcggggtgcg tgctttgaac
      421 acgaccatgc ttgttgagat gggaccggct gcctgggcaa atgttgtcta ctcgtatctt
      481 ctaattctta ttggcgtgct tgtattggct gtaacgttgt atcgttcgcg cggggtctat
      541 cgtatgcagg tgacgtttat cttggcagcg actgtgacgc cctgggtaat tcacatcctc
      601 tttctttcca ttggtgatca tgctctttta cccgacgccg atgtgacacc atttattttc
      661 tcaatgacag cgttactcat tgcatatgct cttattcgtt atcgtttatt ggatgtcgtt
      721 ccgattgctc gcagtattct gatcgagtct atgagcgaag gggtttgcgt cttagattca
      781 cagaaccgca tcgttgacat taatcagaca gcacggaagg cgctctcttc agagttcttt
      841 attgggcagc cagttgaaca agcgttcaag tgctggatag atattgtctc tcattttttc
      901 gatcaagaaa cggcgcagac agaagttgtg attgatgatc gatatcttga tctgcgtatt
      961 tcgcctgtgt atgataaaaa tcaacgcctt ttagggaagt tggttgtctg gcgtgatatt
     1021 acggaattaa agaatacgca gttgaagctt cagcagcttg ccaccatcga tgaattgaca
     1081 ctggcattta atcgccggca ttttatggaa attgccgaag tgcagtttag ccaagcactt
     1141 cggcaaaatc aaccctttgc cttggcgttg atcgatttgg attacttcaa gggaataaat
     1201 gataagtatg ggcaccctgc aggagaccaa gttttagctg agttttcacg catgttcatg
     1261 gagaatatcc gcaaattcga tgtgtttggg cgccttggcg gggaggaatt tgctctgctt
     1321 atgccaaaca ccgatataca ttttgcatat catgcttctg aacggctacg atttatgatt
     1381 accaatacgc ccattccgat caatggcggg gatgtgatag tgacttttag cttgggtgtt
     1441 gctatgcttc aaggcgcaca agatacgctc tctgccatct tccgcagggc agacttggcg
     1501 ctctatgctg caaagaattc tggcaggaat caggtaaaag tatgggagca agggttggaa
     1561 gcagtagcgc caaaacaggc ttaaactcac gccttgctca ttccctgcgg cttgtgtata
     1621 atgccgctat ctaaaagcgt ggatgggaac gagtaaaccg ttcaagacgg gcagcgagtc
     1681 cgaggtcagg tgtgaagcgg atacgttcaa gacggttgaa aatcctcccg gagctgtgaa
     1741 ctgaaatcag aaaattctga gagtagggaa tccgaaattg ttcttcgtta tcagaacagg
     1801 gcgatggttg tcgtccaatg agtgttcatc ttcggatgaa aatcagagtg gtaccgcgtg
     1861 agccttgctc tcgtctctgt tatgagatga gagtttttta ttttaatttc accgtcattg
     1921 cgtggatgat gacagaaaca aggagtaaga atgttcaaag cagtcaaccc aaaactcgat
     1981 gtgccttcga tggaagataa cgtcctcaaa atgtggacga agcaggaaat cttcaagaag
     2041 accgttgacc aacgacaggg caaacctgag tatgtctttt atgaaggtcc gcccaccgcg
     2101 aatggacgcc ccggcgtaca tcatgtgctc gcgcgtgcgt tcaaagatat gttcccgcgc
     2161 tacaagatca tgcgcggata tcacgtctcg cgccgcggcg gctgggacac gcatggctta
     2221 ccggtggaga tcgaagtaga gaagcagctt ggcttcacca acaaacagca gatcgaagaa
     2281 tacggcgttg ataaattcaa cgagctgtgc aaaaaatcgg tcttcaacta catccaggaa
     2341 tgggagaagc tcaccggtcg catcggcttc tgggtggatt tagatactgc ctacgtgact
     2401 tacaccaacg actacatcga gtcggtgtgg tggatcttga aaaacttctg ggagaaggac
     2461 ttgcttttca agggctacaa gatcgtgccg tattgcccgc gttgtggcac gcctctctct
     2521 gaccatgaag ttgctcttgg ctacgaagat acggctgacc cctcagtttt tgtgcgcttc
     2581 ccattggtgg acaagcccga tacttcattc ttagtttgga cgactactcc gtggaccttg
     2641 cctgccaatg ttgccatcgc ggctcacccc gatgtggatt atgtgactgt cgaacg
//
LOCUS       DAOQFT010000351         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_23620_length_2692_cov_6.123246, whole genome shotgun sequence.
ACCESSION   DAOQFT010000351 DAOQFT010000000
VERSION     DAOQFT010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23620_length_2692_cov_6.123246"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..815)
                     /locus_tag="PLR93_09205"
     CDS             complement(120..815)
                     /locus_tag="PLR93_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH27184.1"
                     /translation="MSLQQVVPGFHCINLGFVNAYLLEMQDGLVLIDTGIPNSAEKIL
                     SSVASLGKQPSDIKHILVTHLHGDHTGSLAAIKKATGALVSMHSLDAQLVRIGQAGRP
                     VQPAPGLFNRFIVFMMSRRGPIPIEPAETENELSDGQILEAVAGIKVIHAPGHAAGQV
                     AFLIPIHGGILVAADSCNNRSGLGFPPIFENMDDGIQTLKKLSALDFEVAVFGHGKPI
                     ISGASKAFKQKWG"
     gene            911..2302
                     /locus_tag="PLR93_09210"
     CDS             911..2302
                     /locus_tag="PLR93_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNH27185.1"
                     /translation="MNKNLENDQNLISSLVEYTKDIAQNYLNEIDQRPPATAYAQKQN
                     LDLSLTGIGAEKTLDLFMERYGNHMPASNGPRFWGLVTGGTTPASLLGDWLTSVYDLN
                     LSHAGNSPAPDIETEAISMLRELFGLPASFSGTFVTGATMSNFAGLAIAREWVGKHYG
                     KSVSQEGLHAIPPVKILSCEAHASSFKALGMLGLGRNSLKVIPKLPENREAVNVVELR
                     KALEGMNGEPCIIIANAGTVNTVDFDDIQAIVALKSEFKFWLHVDAAFGGFAACSPKF
                     KHLLNGIEYADSITIDAHKWLNVPYDSAMIFTQHRDLQIAVFQNSGAYLGAIAEPVDF
                     IHLSPENSRRMRALPAWFSLMAYGKEGYQDIVERNCAMAERLGEKISTSKEFRLLAPV
                     RMNVVCFTLSGDVDAEKVKAFLSKLRDDGRVFMTPTTYLGTPGIRAAFSNWRTEEKDI
                     EIAWQAMLDCVSQ"
     gene            complement(2351..>2692)
                     /locus_tag="PLR93_09215"
     CDS             complement(2351..>2692)
                     /locus_tag="PLR93_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27186.1"
                     /translation="ISSIAFSPNGDFLFLRETDQILVVDPLTGNEVSRIRQKGDVADL
                     TLSPDGKMLYSAALRSMQYFDMFARQDISGDEIITAACSRLLKNFSAAEWTLFFEKED
                     YRALCEALPVP"
BASE COUNT          748 a          630 c          631 g          683 t
ORIGIN      
        1 acggcgccga agccgcccag ttcatgcaag cgctggctgg gttcctcgaa aatccggtca
       61 gaatgttggt ataaataaaa aactccgaac aaattgttcg gagtttttta ttggggttct
      121 tagccccatt tttgtttgaa tgcttttgat gcaccgctta tgatcggttt tccatgtcca
      181 aatacggcaa cttcaaaatc caaagcggat aattttttga gtgtttgaat tccatcgtcc
      241 atgttttcaa aaataggcgg gaatcccaaa ccagagcgat tattacaaga atcggcggca
      301 accaaaatcc caccgtgaat gggaatcaag aaagcaacct gccctgcggc atgacccggc
      361 gcatggatca ctttaatccc cgcaaccgct tcaagaattt gcccatcgct taattcattt
      421 tcggtttctg caggttcaat gggaatgggt ccacggcgag acatcataaa aacgataaag
      481 cgattgaaca acccgggtgc aggttgaaca gggcgcccag cctgcccaat tcttaccaac
      541 tgagcatcca gcgaatgcat agaaaccagc gcacccgtcg cctttttgat tgcagccaga
      601 ctgccggtat ggtctccatg caaatgagtg acaaggatat gtttaatgtc gctgggctgt
      661 ttccctaaag atgctacgga agatagtatt ttctcagcgc tattaggaat gccagtatca
      721 atcaagacca atccgtcttg catctccaag agatacgcat ttacaaagcc aaggtttata
      781 caatgaaaac cgggaacaac ttgttgaaga gacatcattc ctcacagaat caagattttt
      841 cgatcacagt attgaggaat atccaatcga gtaaaatctt acgtgaaaat caactctgaa
      901 agaaatcctt atgaacaaaa accttgaaaa tgaccaaaac cttatatctt ctttagtaga
      961 atatacaaaa gatatcgcac aaaactatct taacgaaatt gaccaacgcc cgcctgcaac
     1021 agcgtatgct caaaaacaaa accttgatct ctcccttacg ggaatcggcg cagaaaaaac
     1081 attggacctt ttcatggagc gctacggaaa tcacatgccc gccagcaatg gaccgcgatt
     1141 ctggggtttg gtcactggcg gcacgactcc cgcttccctg ctcggggatt ggctgacttc
     1201 tgtttacgat ctgaatttga gccacgctgg gaattctccc gcgccggata ttgaaacgga
     1261 agcgatttcc atgctgcgcg agttattcgg tttgcccgcc tctttctcag ggacgtttgt
     1321 taccggcgcg acgatgtcta acttcgccgg tcttgccatt gctcgcgaat gggttgggaa
     1381 gcattatgga aaatcggttt cgcaagaagg gttgcatgcc attccacccg tcaaaatatt
     1441 aagttgtgaa gctcatgcca gctcattcaa ggcattggga atgcttgggc tgggaagaaa
     1501 ctccctgaaa gtcatcccca agcttcccga aaatagagaa gcggtgaacg tcgtcgaatt
     1561 gcgaaaagct ttggaaggga tgaatggcga accctgcatc attatcgcca atgcagggac
     1621 ggtcaacacc gtggacttcg atgacatcca agccattgtg gcgttgaaat ctgaattcaa
     1681 gttctggctg catgtggatg cggcatttgg cgggtttgcc gcgtgttcac caaaattcaa
     1741 gcatttgctc aacgggattg aatatgcaga cagtatcaca atcgatgcgc ataaatggct
     1801 gaatgtgcct tacgactcgg caatgatctt tacgcaacat cgcgacttgc aaattgcagt
     1861 ttttcaaaac agtggcgctt atcttggtgc aattgccgaa ccggttgatt ttatccatct
     1921 ctcgccagaa aactcgcgcc gtatgcgtgc actgcctgcc tggtttagtt tgatggcata
     1981 tggcaaagaa ggttatcaag acattgtgga aagaaattgc gctatggcag agagactcgg
     2041 cgagaagatt tcaaccagca aggaattccg tctgctcgcg ccagtgcgga tgaacgtagt
     2101 ctgttttacg ctgagcggtg atgtggatgc ggaaaaggtc aaggctttct tgtctaaact
     2161 acgggacgat ggacgcgttt tcatgacacc cactacttat ctgggcacac ccggaatccg
     2221 cgcggcattt agcaattggc gtaccgaaga aaaagatatc gagatcgcct ggcaagcaat
     2281 gctagattgt gtaagccaat agaatcaaaa aaactccgaa gcattcgctt cggagttttt
     2341 tgaatttact ttatggcaca ggcaaagctt cgcacagcgc acgataatct tctttttcaa
     2401 agaataatgt ccactccgct gcgctaaagt tctttaagag gcgactacac gcggctgtga
     2461 tgatctcgtc acctgagatg tcttgcctgg caaacatatc aaaatattgc atagaccgca
     2521 aagctgcaga atatagcatt ttgccatctg gggaaagtgt gagatctgcc acatctccct
     2581 tttggcgaat gcggctgact tcgttcccag tgagtgggtc aactactaga atttggtcgg
     2641 tttccctaag gaagagaaaa tctccattgg gcgaaaatgc tatcgaagaa at
//
LOCUS       DAOQFT010000352         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_23700_length_2685_cov_7.265019, whole genome shotgun sequence.
ACCESSION   DAOQFT010000352 DAOQFT010000000
VERSION     DAOQFT010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23700_length_2685_cov_7.265019"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLR93_09220"
     CDS             complement(<1..251)
                     /locus_tag="PLR93_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH27187.1"
                     /translation="MQLSFSKQDNVTVLHVMGDVDSSNYTEVIIKAQQLFDDGTRNLL
                     LDLANVPYVSSAGLMAFHTIARVFAGHSIQGGESGRPTFR"
     gene            complement(268..1545)
                     /locus_tag="PLR93_09225"
     CDS             complement(268..1545)
                     /locus_tag="PLR93_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNH27188.1"
                     /translation="MKRNLGNIVFTILVLLTIVVWVVFPPHSEGVENYLRYHIGMTMG
                     SVVIALMSFSLFISTRPRWAEPYFGGLDKMYMTHRYTSTAAFLLMFVHLLIVPLHLVN
                     LALGNYLAIVAFLGFIAIVLPTLAPRIPFLNKLTSGTYEGWKRLHGFIGIFFILGYLH
                     SITVNAPTTKIAINWNQIFVFLGIGSYIYTEVLSRFLGKHVPYTVKAVNHPNNSTTEV
                     VMQAKGKPIQHAHAGQFLFVRFKGDKILDEAHPFTISSAPHEGELRVTVKAVGDFTRY
                     LFQNLKPGMDAIVEGAYGMFNYKTGSDKQIWVAGGIGITPFLSFLRDLKTDLAHDVHF
                     YYTVRHPEEAVFADEIEAIAKKHPRLKAHIRFSSQHGSLTVDEIVKNAGGDASKHHVY
                     MCGPLPMVQAFASKFVEAGVSKDNVHFEEFNFR"
     gene            complement(1613..2026)
                     /locus_tag="PLR93_09230"
     CDS             complement(1613..2026)
                     /locus_tag="PLR93_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH27189.1"
                     /translation="MEINISQEKGNVPVTVIQVTGQLDGQTYQKLIAAAQEVIKEGST
                     NILLDLSELTFISSAGLVALHVIALLLRGEAMPDLDHGWAALKSVNKSRESGVQQHVK
                     LLNPREEVINVLDMVGFSSFFEVFKDKQKALESFS"
     gene            complement(2062..>2685)
                     /locus_tag="PLR93_09235"
     CDS             complement(2062..>2685)
                     /locus_tag="PLR93_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNH27190.1"
                     /translation="RALKYIEEHVMGFTRSSEEVPGYEIPWMYFDYLRTGDARPLGGV
                     FYHNAMDVVAMAALLAHVSEILADPYNGRVEHGLDFIALGKLFEDLGHWDEAARLFER
                     GLEIGLDESDFGVAVKRISILQKKRGDMDRAVELWKAAAKKGHIYAHIELAKYYEHKM
                     RDAKAAVKWAKSARAEAEKADLPPYIRNHWFHEIDHRLERLTRKAGL"
BASE COUNT          741 a          698 c          578 g          668 t
ORIGIN      
        1 cggaaggtgg gtctgccact ttccccgcct tgaatggaat gccccgcaaa cacacgcgca
       61 atggtatgga atgccatcag cccggcgcta ctcacatacg gtacattggc aaggtccagc
      121 aacaggttgc gagtgccgtc atcaaaaagt tgctgcgctt tgatgatgac ctccgtgtaa
      181 ttagacgagt ctacatcccc catgacatgc aagaccgtta cattatcttg tttcgaaaaa
      241 gagagttgca tgggtttact cctacgacta tctgaaattg aattcctcaa agtggacgtt
      301 atctttggac acgcccgctt ctacaaattt tgacgcgaac gcctgcacca tgggcaaagg
      361 accgcacata tagacgtgat gtttggaggc atctccacct gcatttttga cgatctcatc
      421 cacagtcaat gaaccatgct gagaagagaa gcggatgtgt gccttcaaac gcggatgttt
      481 ctttgcaatg gcttcgatct catctgcaaa gactgcttct tcagggtgtc ttaccgtgta
      541 gtaaaaatgc acatcgtgag cgagatccgt tttcaggtcc cgtaagaacg agaggaaagg
      601 ggtaatgcca atgccgccag cgacccaaat ctgtttgtca ctacctgttt tgtaattgaa
      661 catcccatat gcgccttcta caatcgcatc catgccgggt ttgagattct gaaacaggta
      721 acgggtgaaa tcaccgacag ctttaaccgt cactcgcaat tcgccttcat gcggcgcact
      781 cgaaatggtg aagggatggg cttcatccag aattttatcg cctttgaagc gcacaaacag
      841 aaattgaccc gcatgagcat gttggatggg cttgcccttg gcttgcatca ccacctcagt
      901 ggtggagtta ttgggatgat ttaccgcttt caccgtataa ggcacatgct tgcctagaaa
      961 gcggctcaac acttctgtgt aaatgtagga accaatacca aggaaaacaa agatctgatt
     1021 ccagtttatg gcaatctttg ttgttggggc attcaccgtg atcgagtgta gataacccaa
     1081 aatgaagaaa ataccaataa agccatgcaa acgcttccag ccttcatagg ttccgctcgt
     1141 gagtttattc aagaacggga tgcgtggcgc gagcgtaggt aaaacgatgg cgataaaacc
     1201 gagaaatgca acgatcgcta gataatttcc aagcgcaagg tttaccagat gcaatggcac
     1261 gatcaacaag tgaacaaaca ttagcaagaa tgcggcagtc gatgtatagc gatgggtcat
     1321 gtacatctta tccaagccac caaagtatgg ctctgcccag cgcggacgag tggaaatgaa
     1381 cagcgagaac gacatcaagg cgatcacaac agaccccatc gtcatcccga tgtgatagcg
     1441 tagataattt tcaacccctt cactgtgcgg cggaaagaca acccacacga cgatggtaag
     1501 taagaccaaa atagtaaaga caatattccc aagattacgt ttcataaaaa ttcaccttta
     1561 atataaatga caacgatgca ggcatgcctg catcgttgtg ggtcgtttct tcttacgaga
     1621 aagattcgag agccttttgt ttatctttga aaacctcaaa gaaagaagag aagccaacca
     1681 tatcgagcac gttgatcact tcttcgcgcg ggttcagcaa tttcacgtgt tgttgaactc
     1741 cactctcacg tgatttgttg accgacttca acgccgccca accatggtca aggtctggca
     1801 tggcttcgcc gcgcaacagc agggcaatca catgcagcgc caccaaccct gcgctggaaa
     1861 taaaagttag ctcactgaga tccaataaaa tattcgttga gccttctttg ataacttcct
     1921 gagcagcagc gatcaatttc tgatacgtct gcccgtctaa ttgacccgtc acctgaatca
     1981 cagtaaccgg cacattcccc ttttcttgtg aaatgttaat ttccatgttt cctctccaaa
     2041 ggttaatccg tgttcgagca attataaacc cgctttacgc gtcaaccttt ccaaccgatg
     2101 gtcaatttca tggaaccaat gattacgaat ataaggaggc aaatccgctt tctcggcttc
     2161 tgcccgtgca gacttcgccc acttgaccgc agcctttgca tcgcgcatct tatgctcata
     2221 atattttgcc aattcaatat gagcataaat atgacctttc ttcgccgccg ctttccacaa
     2281 ctcgacagct cggtccatat ctccacgctt cttttgcaag atggagatcc gcttcactgc
     2341 tacgccgaaa tctgattcat ccaacccgat ctcgagtcca cgctcgaaca gacgtgccgc
     2401 ctcatcccaa tgtccaaggt cctcaaatag cttccccagt gcaatgaaat caagcccatg
     2461 ctccacacgt ccattatagg ggtctgccaa aatctcgctg acgtgtgcca gcaacgccgc
     2521 catcgctacc acatccatgg cattgtgata gaacacacct ccaagtggac gggcatcgcc
     2581 tgtgcgtaag taatcgaaat acatccacgg aatttcataa ccgggtactt cttcagaaga
     2641 acgcgtaaac cccattacat gttcttcaat atatttcaat gcacg
//
LOCUS       DAOQFT010000353         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_23930_length_2670_cov_4.788910, whole genome shotgun sequence.
ACCESSION   DAOQFT010000353 DAOQFT010000000
VERSION     DAOQFT010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23930_length_2670_cov_4.788910"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLR93_09240"
     CDS             <1..998
                     /locus_tag="PLR93_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH27191.1"
                     /translation="LLNRAAQGQLPLGSVFKIITFSAALESGLFLKDTTYNCQYDFTE
                     LQDQVRHDWTWQHCQDRLAGGLFCNTSDSTPSGLLTLQEGLMRSCNPYFWHIGNDLFT
                     NWDRGNDIANMARAFGLGSPTGIQQIPEEAGQIDDPATQVDAVNQAIGQGTVLVTPLQ
                     VVRFMAAIANGGTLYRPQIVEKIQPIDGDPIQSFKPEAQGTLPLRPENLDILREALLM
                     VVENPKGTARFNMRGLQFAVAGKTGTAESGSGKPHGWFAGYTMNEENTGLPDIAIVAV
                     GENIGEGSEYAVPFFRAMVETYYYGSPQRQYYDWGQIGYPPYTPTPPGGGVIPGG"
     gene            1039..1938
                     /gene="rsgA"
                     /locus_tag="PLR93_09245"
     CDS             1039..1938
                     /gene="rsgA"
                     /locus_tag="PLR93_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNH27192.1"
                     /translation="MANTKQGLIVKAQSGFFWVETGEGVIVCQLRGKLKQGRAKGDIA
                     ALGDQVDITVLDDGSGVVETVRERKQLIARLDPRPGGVYQQVLLANPDQAVFVFACAN
                     PNPKLRMLDRFLVIAEKQKLPVVIVANKIDLVANPKDSFGMYEAIGYHVIYTSTKSET
                     GLIELRNQLRNKLSAFAGPSGVGKSSLLNALQPGLGLAVNEISSVMNKGRHTTVVRQM
                     FAFEGGYVADTPGWKSLGLWDTEPEEIDAYFPELRGLVEQCQFSDCTHQHEPGCAVLT
                     ALKDGKIHVERYESFVRLRSGKE"
     gene            2018..>2670
                     /gene="holB"
                     /locus_tag="PLR93_09250"
     CDS             2018..>2670
                     /gene="holB"
                     /locus_tag="PLR93_09250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNH27193.1"
                     /translation="MTDNWNLLGHEWAVDMLRRHVARGEMRHAYLFAGAPGLGRRTLA
                     LRLAQALNCEKPVAPGEPCFVCRTCKQIDAMQHPDLAIIQAVDEDGVGKDGGTLRVDQ
                     IREVQRTLNLKPYQSPFRVALFLRFHEANDNAANALLKTLEEAPAHAILLLTADNPEQ
                     LLPTINSRCEILRLRPLPLESIVADLLDHGVDEDRARLLAHISGGRPGFARKLLEDAT
                     "
BASE COUNT          658 a          631 c          692 g          689 t
ORIGIN      
        1 cgcttctgaa ccgtgctgct cagggtcaac tcccattagg gtctgttttc aagatcatta
       61 ccttctcggc agctcttgaa agcggattgt tcttaaaaga tacaacatat aattgtcagt
      121 atgattttac agaactacaa gaccaagttc ggcatgactg gacgtggcag cactgtcagg
      181 accgtcttgc cggcgggtta ttctgtaaca cgtctgatag caccccctcg ggtttgctta
      241 ctttacaaga aggtcttatg cgttcttgta acccatactt ctggcacatt ggcaacgacc
      301 tctttacaaa ctgggaccgt ggcaacgaca ttgcgaacat ggcacgtgca tttggtttgg
      361 gttctcccac cggcattcag cagattcctg aagaagcagg tcagatcgat gacccagcca
      421 ctcaggtaga tgcagtcaac caagcaattg gtcagggcac ggttttagtc acacctttgc
      481 aggtggttcg ctttatggca gctattgcca atggcggtac tttataccgt ccacagattg
      541 ttgaaaagat tcagcctatc gatggcgacc ccattcaatc ctttaagcca gaagcgcaag
      601 ggacgctccc attgcgccca gaaaatttgg atattctgcg agaagcttta ttgatggtgg
      661 ttgagaaccc caaaggaacg gctcgcttca acatgcgcgg acttcaattt gcagtggctg
      721 gaaaaaccgg cactgccgag tctggtagtg gtaaaccgca tggctggttt gcggggtaca
      781 ccatgaacga agaaaataca ggtttgcctg atatcgccat tgtggcagta ggtgagaaca
      841 ttggtgaggg ttctgaatat gccgtaccgt tcttccgcgc tatggtagaa acctattact
      901 atggcagccc tcaacgccaa tactatgatt ggggtcagat cggttacccg ccttatacgc
      961 ccactccgcc cggcggcggc gttattccgg gaggataaca tcaagaggca ggtatatacc
     1021 tgcctctaag gaattcactt ggcaaatacc aaacaaggac tcattgtaaa agcccaatcc
     1081 ggtttctttt gggtagaaac cggggagggc gttattgttt gtcaattacg aggcaaactc
     1141 aagcaaggca gagccaaagg cgatattgca gctttgggag atcaagtgga tatcacggtg
     1201 ttggatgacg gttccggcgt ggttgaaact gtccgtgagc gcaaacaact catcgcgcgg
     1261 ctcgatccgc gcccgggcgg ggtctatcaa caggtgcttt tagccaatcc cgatcaggct
     1321 gtcttcgtct tcgcatgcgc gaatccaaat cccaaattga gaatgctcga ccggtttctt
     1381 gttattgcag aaaagcaaaa acttcctgtc gtaatcgtgg cgaataagat tgacctcgtt
     1441 gcgaatccaa aagatagttt tggaatgtac gaagctattg gctatcacgt aatttataca
     1501 tccacgaaaa gcgagacggg tcttatagaa cttcgcaatc aattgcggaa caagctcagt
     1561 gcttttgccg gacccagcgg tgtgggcaag tcgagtttat tgaacgcttt acagcctggt
     1621 ttgggtttgg ctgtgaacga gattagttct gtgatgaata agggacgtca taccacggtg
     1681 gtgcgccaaa tgtttgcctt tgaaggtggc tatgtcgcag atacacctgg ttggaagtct
     1741 cttggcttgt gggataccga acccgaagaa attgacgctt atttccctga gttgcgtggg
     1801 ttggtggagc aatgtcaatt tagtgattgt actcatcaac atgagccagg ttgtgctgtg
     1861 cttacagcgt tgaaagacgg taagattcat gtagaacgat atgagtcttt tgtaagacta
     1921 cgttctggca aagaataacg tgtagaaaac taccaaatat gtattgcatg gtttccacca
     1981 cttgacactt aatctctgac ccctgatact taaaccaatg actgataact ggaatttact
     2041 cgggcacgaa tgggcagtcg atatgcttcg ccgccatgta gcgcgtggag agatgcgcca
     2101 tgcgtacctt tttgcaggcg ctccgggttt ggggcgccgg acgttggcat tgcgcctggc
     2161 acaagctttg aactgcgaga aacccgttgc accgggtgaa ccttgttttg tgtgccgcac
     2221 ctgcaaacag atcgacgcca tgcaacaccc agatttagcg atcattcaag ctgtggatga
     2281 agatggcgtg gggaaggatg gcggcacttt gcgcgtggat caaatccgtg aagtgcagag
     2341 gacgttgaac cttaagccgt atcaatctcc gtttcgtgtg gcattgttct tgcgttttca
     2401 cgaagcgaac gataacgccg ccaatgcgtt gctcaaaaca ttggaagaag ccccagctca
     2461 tgccattttg ctcttaactg cagataaccc cgaacagctt ttaccaacca tcaactcgcg
     2521 ctgtgaaatt ttgcgtctac gtccgttgcc actcgaaagt attgttgcag accttctcga
     2581 tcacggtgtg gacgaagacc gtgcccgttt gctggcgcac atctcgggtg gtcgccccgg
     2641 ctttgcacgt aaattattgg aagatgccac
//
LOCUS       DAOQFT010000354         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_24082_length_2660_cov_7.975048, whole genome shotgun sequence.
ACCESSION   DAOQFT010000354 DAOQFT010000000
VERSION     DAOQFT010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24082_length_2660_cov_7.975048"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..752
                     /gene="ccsA"
                     /locus_tag="PLR93_09255"
     CDS             54..752
                     /gene="ccsA"
                     /locus_tag="PLR93_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNH27194.1"
                     /translation="MQSKPIALTILDVLSVIGIGVATYFALVFAPTEAVMGNVQRVFY
                     FHVGTAWVGLIGFVVAAVTGIIYLVTKDMKWDRVEVAAVEVSTMFFFITIVLGSIWAR
                     PAWNTWWTWDPRLTTAAVTELIYIAYFMLRAGMEDPEKRARFGAVYALLGGLSAPITF
                     MVIRLFRTIHPVVVGNQSAAAQGGFDMSGNMLTAMFVALGVFTIIFIDLMWHRIRMGN
                     LEEQVEQLKLKVSM"
     gene            764..916
                     /locus_tag="PLR93_09260"
     CDS             764..916
                     /locus_tag="PLR93_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27195.1"
                     /translation="MGPANTDNYMIAGYVVSYITMGIYLLSMYIRNRNLKRDLETYQE
                     MDKEKK"
     gene            980..2638
                     /locus_tag="PLR93_09265"
     CDS             980..2638
                     /locus_tag="PLR93_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27196.1"
                     /translation="MPRRHWANRLLLVLIAVSNLTVSYGAVPASSINPAPVSESNPPP
                     GPDRFSVIAVEYQSYNWELRQWKNRKVVCNLTIDHDGMPSPDEVYRDCGTIIYRNWMA
                     QPMCNVNNVANCEGYYAKLIDTDTKEKEIGMQLPAASAWISLEDCEPVYSTSTNICET
                     KPTLVITGQEPLPNESIVRVEGTYDQQAFFCDETDVCKFRIPETGDEGVKVEFWSYSS
                     YGDSSVVYEAQVRVKQVDEGDPDQLYWYVDVLSPQWQGEVSATCAETWGVFPPVGGAP
                     EWLSTPPQSEDLSSDIPYTYLAANLILQGVVDARSCPDGGLSPGNVANQCGLEKSRDA
                     VNEWQNQFDGLILETAKKTSVPAFLLKNLFARESQFWPGIFQAVGDIGLGQLTENGAD
                     TALFWNKSFYNQFCPLVMDSETCGEGYANLEEEQQVQLRRALVGSVDASCEDCPLGLD
                     LTQADFSVGVFANTLIANCEQTAQVIYNYTGSQTSKAASYEDLWKFTLVNYNAGGGCL
                     AEAISGANGTSGLPLTWETVSPNLIGTCASAVDYVNDISKETNP"
BASE COUNT          708 a          653 c          630 g          669 t
ORIGIN      
        1 ttatttttta atcaaatagt agcaatcgta aatttataaa ggagatctat cctatgcaat
       61 ctaaaccaat tgctcttacc attctcgatg tactctctgt gatcggaatt ggtgttgcca
      121 cttacttcgc tctggtcttc gcccccacag aagctgtaat gggcaacgtc caacgtgtgt
      181 tttactttca tgtaggaaca gcttgggtcg ggctgatcgg cttcgtcgtt gccgccgtca
      241 ctggcatcat ctacctcgtc accaaagata tgaaatggga ccgggttgaa gtcgccgctg
      301 tggaagtaag caccatgttc ttcttcatca ccatcgtgct tggctccatt tgggctcgcc
      361 ccgcttggaa cacctggtgg acttgggacc cgcgcctcac caccgccgcc gtaacggaac
      421 tcatctatat tgcctacttc atgctccgcg ccggaatgga agaccccgag aaacgtgcac
      481 gcttcggtgc cgtgtatgcc ctcttgggcg ggttgagcgc gccgattaca ttcatggtca
      541 ttcgcctttt ccgcaccatt cacccggttg tggttgggaa tcagagtgcg gctgcccaag
      601 gcggcttcga catgagcggc aatatgctca ccgccatgtt cgtagccctc ggcgtattca
      661 ccatcatttt cattgacttg atgtggcatc gcattcgcat gggcaatttg gaagagcaag
      721 tagaacaact taaactgaaa gtttccatgt aaaggagcca acaatgggac ccgcaaatac
      781 tgataactat atgatcgctg gctacgtggt gtcgtatatc accatgggca tctatctact
      841 cagcatgtat attcgtaacc gcaacctaaa acgcgacctc gaaacctatc aagagatgga
      901 taaagagaaa aaataaatct aaagaagggt ccgtcacaag atatctacaa ggtataatgt
      961 gacggacttt ttgattctca tgccaagacg ccactgggca aaccgtcttc tcctcgtgtt
     1021 gatcgcagtc tcaaacctga cggtcagtta tggagccgtg cctgcttcca gcatcaaccc
     1081 tgcgcctgtc tccgagtcca atcccccacc aggtccagac cgtttctcgg ttatcgctgt
     1141 tgaatatcaa tcgtacaact gggaattgcg ccaatggaaa aaccgtaagg tcgtctgtaa
     1201 tctcaccatc gaccacgatg gcatgccttc tccagatgaa gtatatagag actgtggcac
     1261 catcatctat cgcaactgga tggcacagcc catgtgcaat gtcaataatg tcgcaaactg
     1321 cgaggggtat tacgcgaagc ttattgatac agatacgaaa gaaaaagaga tcggcatgca
     1381 actacctgcc gcaagtgcat ggatctctct tgaagattgt gaaccggtat atagtacatc
     1441 taccaatatt tgtgaaacca aacctactct tgttattacc gggcaggaac cccttccaaa
     1501 cgaatcgatt gtccgcgtag aaggaacgta tgatcagcaa gctttcttct gcgatgagac
     1561 tgatgtctgt aagttccgca tccctgaaac aggcgatgaa ggtgttaaag ttgaattttg
     1621 gtcatactca tcgtatgggg atagcagtgt cgtatatgaa gcacaagtgc gtgtcaaaca
     1681 agtggacgaa ggcgacccag accagcttta ttggtatgta gatgtacttt cgccacaatg
     1741 gcaaggtgaa gtcagtgcaa cttgtgctga gacatgggga gtctttccac ctgtaggcgg
     1801 cgcacctgaa tggctcagca cccccccaca aagcgaagac ttaagcagtg atattccata
     1861 tacatatctt gcagcgaatc tcattcttca aggcgtcgtt gatgctcgct cctgccccga
     1921 tggcggctta tcacccggca atgttgccaa tcaatgcggg cttgaaaaat cacgtgatgc
     1981 agttaatgaa tggcaaaatc aattcgatgg gttaattctt gaaacagcca aaaagacaag
     2041 cgtccctgca tttttattaa agaatctttt tgcacgagaa agccaattct ggcccggcat
     2101 cttccaagcc gttggggata ttggacttgg gcaactcact gagaacgggg cagatacggc
     2161 tctcttttgg aataaatctt tctacaatca attctgtccg ttggtgatgg attcagaaac
     2221 atgcggcgaa gggtatgcta acctggagga agagcaacag gtccagctaa gacgcgcgct
     2281 cgttggcagt gtggatgcgt cttgtgaaga ttgtccgctc ggtttagatc tcacacaagc
     2341 agatttctca gtcggtgtct ttgcaaacac actgatcgcc aattgcgaac aaactgcaca
     2401 agttatttac aactacacag gttctcaaac cagcaaagcc gcatcctatg aagacttgtg
     2461 gaagttcacc cttgtcaatt acaacgcggg cggaggatgt ttggctgaag ccatcagtgg
     2521 cgcaaacggc acaagcggat tgcctctcac atgggaaacc gtctcaccaa atttaattgg
     2581 aacatgtgca agcgcagtgg attatgtcaa cgacattagc aaggagacaa atccataatt
     2641 cgggggataa aaggaagaac
//
LOCUS       DAOQFT010000355         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_24191_length_2653_cov_6.717090, whole genome shotgun sequence.
ACCESSION   DAOQFT010000355 DAOQFT010000000
VERSION     DAOQFT010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24191_length_2653_cov_6.717090"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLR93_09270"
     CDS             <1..404
                     /locus_tag="PLR93_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNH27197.1"
                     /translation="RMDRHVQNAQAVAEFLEKHPKVSWVKYPGLKSHPDYDRAKKYLP
                     KGAGAILGFGIKGGAAAGKKFIESLQLFSHLANVGDARSLAIHPATTTHSQLTEEQQV
                     TAGVSPDFVRLSIGLEDFEDIKWDLDQALNA"
     gene            476..1402
                     /gene="metA"
                     /locus_tag="PLR93_09275"
     CDS             476..1402
                     /gene="metA"
                     /locus_tag="PLR93_09275"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNH27198.1"
                     /translation="MPVKIPSTLPAREILEYENIFVIDEERALHQDIRALRVAILNLM
                     PTKVATETQILRLLSNSPLQVEITLVHTATHESKNTDAEHLLQHYVTFEDIKHEKFDG
                     LIITGAPVEQLPFEEVDYWEELKQVMDWAKTNTESTFYICWGAQAGLYHYYGVPKYDL
                     PQKMFGVFEHRLLNRTESLMRGFDDIFLAPHSRHTEIRREDIEKVPELQILAESEDAG
                     VYIVGSRDGRELFITGHSEYDPLTLKAEYDRDVNKGLPIHVPKNYYPNDDPKQMPNVR
                     WRGHANLLYANWLNYYVYQATPFDPNEIPKRN"
     gene            1402..>2653
                     /locus_tag="PLR93_09280"
     CDS             1402..>2653
                     /locus_tag="PLR93_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNH27199.1"
                     /translation="MTSKHNFGFTTRQIHEGYTPDPATGSRAVPIYQTTAYDLQSTDR
                     AARLFALQENGNIYTRISNPTSNVFEKRVASLEGGIGALEVGSGHAAQATAILTICEA
                     GDHIVSTSLLYGGTVTQFKYTFPKLGITVTLVDPSDPENFRRAIQPNTKIIYGETISN
                     PLGNIFPFEEVSKIAHEHGIPLMIDNTFATPYLCRPFEWGADIVTHSTTKFIGGHGTT
                     MGGIIVDSGKFNWSKSGRFKNFTEPDPAYHHVIYADDFGPLAFISKARAGILRDLGTV
                     QQPIASWFLIQGLETLSLRMTRHVQNAKRAAEFLENHPNVKWVSYAGLSSHPDHERAQ
                     KYLPQGTGAVLGFGVKGGREAGAKFIDNLQLFSHVANIGDTRSLAIHPASTTHSQLSD
                     EEQTAAGVSPDFIRLSIGLEDIEDI"
BASE COUNT          729 a          755 c          575 g          594 t
ORIGIN      
        1 tgcgcatgga tcgtcacgtg cagaacgcgc aagccgttgc tgaattcctt gagaaacatc
       61 ctaaagtcag ctgggtcaaa tatcctgggc tcaagagcca cccagactac gaccgcgcca
      121 agaagtattt gcccaaaggc gcaggtgcga ttctcgggtt cggtatcaag ggcggtgcag
      181 ccgcgggcaa gaagttcatt gaaagcctgc agctcttcag ccaccttgcc aacgttggtg
      241 atgcccgctc tctcgccatt catcccgcta ccaccactca cagtcagttg accgaagaac
      301 aacaggtcac cgctggcgtc agtcctgatt tcgtccgctt gagcattggt ctcgaagatt
      361 tcgaagacat caagtgggat ctcgatcagg cgcttaacgc ctaaacatag aaattgcatg
      421 tggaatgaag aaaaaaattc ttcattccac atgcataaat catcatttca aactcatgcc
      481 tgtaaaaatc ccttctactc ttcctgctcg tgaaattctc gaatacgaga atatcttcgt
      541 catcgacgaa gagcgcgcct tacatcaaga catccgtgcc ttgcgtgtgg caattctgaa
      601 tctaatgcca accaaggtcg ctacagaaac acagattctg cgcttacttt caaactctcc
      661 gttgcaagtg gagatcacac tagttcacac cgctacccac gaatccaaga acacagatgc
      721 agaacatctg ctgcaacatt atgtcacctt tgaagatatc aagcatgaaa aattcgatgg
      781 gctcattatc acgggtgcgc cagtggaaca actccccttt gaagaagtag attactggga
      841 agaactcaaa caggtgatgg attgggcaaa aaccaatacc gagtccacgt tttatatttg
      901 ttggggagcc caagccggac tctatcatta ctacggcgtc cccaaatatg acctgccgca
      961 aaaaatgttc ggcgtttttg aacaccgcct cttaaaccgg accgaaagcc tgatgcgcgg
     1021 ctttgacgac attttcctcg ctccacactc ccgccatact gagattcgcc gcgaagatat
     1081 tgaaaaggtg cctgaattac agatcctcgc cgaatcagaa gacgcaggtg tttacatcgt
     1141 cggttcaaga gatggacgtg agctctttat tacgggtcac tctgaatacg accctctcac
     1201 acttaaagca gaatacgacc gtgatgtgaa caaggggtta cctattcacg ttccgaaaaa
     1261 ttattatcca aatgatgacc ccaagcaaat gccaaacgtg cgctggcgcg ggcatgcgaa
     1321 tttactgtac gccaactggt tgaactatta cgtataccaa gcaactccct ttgacccaaa
     1381 tgaaattcca aagcggaact aatgacttca aaacataact tcggcttcac cactaggcaa
     1441 atccacgaag gctacactcc agaccccgct accggtagtc gggctgttcc gatctaccaa
     1501 accacagcct acgacctgca aagcacagac cgtgccgcaa gactctttgc ccttcaagaa
     1561 aacggtaata tctacacccg catctcaaac ccaaccagca acgtcttcga gaagcgcgtc
     1621 gccagcctcg agggcggaat cggcgcgctt gaagttggct ctggtcatgc cgcgcaagct
     1681 accgccatcc tcactatttg cgaagccggt gaccatatcg tctccacctc cctgctgtat
     1741 ggcggaacgg tcacccaatt caaatacaca ttcccaaaac ttggaatcac cgtcactctc
     1801 gtagacccaa gcgacccgga aaactttcgt cgcgccattc agccaaatac aaaaataatc
     1861 tacggcgaaa ccatcagcaa cccgcttggc aatatctttc cattcgaaga agtctcgaag
     1921 atcgcacacg aacatggcat cccgctcatg atcgataata ccttcgccac gccctacttg
     1981 tgtcgtccct tcgaatgggg tgcagatatc gtcacccact ccaccaccaa attcatcggc
     2041 gggcacggta ccaccatggg cggaattatc gtagatagtg gcaaattcaa ttggagtaag
     2101 agcgggcgct tcaaaaactt caccgagcct gatcctgcat accatcacgt tatctacgcc
     2161 gatgactttg gtccattggc gttcatctcc aaagcacgcg ctggtatctt acgagactta
     2221 ggtaccgtcc aacagccaat tgcatcctgg tttcttattc aagggttaga aaccttatcg
     2281 ttgcgaatga cacgtcacgt gcaaaatgcg aaacgagccg cagagtttct tgagaatcat
     2341 cccaatgtga aatgggtttc atacgcaggt ttatccagtc acccagacca tgaacgcgcc
     2401 cagaaatatt taccccaagg cacaggcgct gtactcgggt ttggagtcaa aggcgggcgt
     2461 gaagcaggag cgaagttcat cgataacctg caactcttca gccacgtcgc caacattggt
     2521 gacacgcgct cgctggcgat ccatcccgcc agcaccaccc acagccaact ctccgacgaa
     2581 gagcaaaccg ccgcaggtgt cagccctgat ttcattcgtt tatcgattgg cttggaagat
     2641 atcgaagata ttc
//
LOCUS       DAOQFT010000356         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_24236_length_2650_cov_9.128324, whole genome shotgun sequence.
ACCESSION   DAOQFT010000356 DAOQFT010000000
VERSION     DAOQFT010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24236_length_2650_cov_9.128324"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..893
                     /locus_tag="PLR93_09285"
     CDS             99..893
                     /locus_tag="PLR93_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27200.1"
                     /translation="MNIEINDLRFTYPTGLEALKGINLTIQSGEQVAIVGQNGAGKTT
                     LVRHFNGLLRPSSGTVLIGDWETSKYSTAKLASRVGYVFQNPDEQLFSRDVLTEVAFG
                     PRNLGYAKEKVDDLVKRALSLTELSDKTETNPYDLSPTWRKMVALASIIAMDTPIVIF
                     DEPTTGQDAVNVGRIAHVIAELKREGKTVITITHDIDFCAENFDRVIALSKGQILLDG
                     IARDVLGQEEILSQTYVDPPQLTRLGKALGLKETVTKRDEFLKAIE"
     gene            complement(933..1856)
                     /locus_tag="PLR93_09290"
     CDS             complement(933..1856)
                     /locus_tag="PLR93_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNH27201.1"
                     /translation="MPRKVKLILNPMADMGRAWKTANALRPIAQEFQGELTWSGTVYP
                     THAIELARQAAEEGYDMVIAMGGDGTVHEVMSGLMQVPAEKRPVMGVVPIGSGNDFAY
                     SIGIHQKPDHALAHALKAENIKPVDIGLMTDEHGRKEYFDNTLGIGFDAVVTIRSHKL
                     PIVKGFLMYLTAVIQTILLNHNPAKLNIETDHQSWSASALMLTLCNGPREGGGFMLSP
                     KSKNDDGTMEFLIVNKVSRAMMFRLVPEFMSGTHMRFKQIQMGEFKKFSLTSDSPLYI
                     HADGEIFTSFGSNLKKVAFEILPHALKVVHG"
     gene            complement(1928..>2650)
                     /locus_tag="PLR93_09295"
     CDS             complement(1928..>2650)
                     /locus_tag="PLR93_09295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNH27202.1"
                     /translation="LTGSIIGVPLKFKDEVVGVMNLSKKVVGKFTRSELRLINLLADQ
                     AAVAIFNASMYKRLAQMANTDSVTGLPNRRALDERLQEEWRLAEQTKFPFAVVMMDLD
                     GFKAVNDTFGHNIGDDLLYSLFNFLAQRMRSSDFLARYGGDELTLVMRNTDAEAAQSV
                     MKKILELMQDYHFPFPKGKDMKLGITAGIAIYPTHANTPSDLIRAADSALYHAKKYAR
                     GEYVVAKAITGRLNPVQVKDGP"
BASE COUNT          728 a          710 c          547 g          665 t
ORIGIN      
        1 cggactatgg aaaaaggaaa cattacccgt cacgttagat gaggcggtgg aagggtttaa
       61 gcgtcatgcc taatctgcat gatgacaaat aatcaaccat gaacatcgag atcaacgacc
      121 tccgctttac atatcccacc gggttagaag ccctcaaagg catcaacctc accatccaat
      181 caggggaaca agttgccatt gtcggtcaga atggggctgg caagaccaca ttagtgcgtc
      241 acttcaatgg cttactacgc cccagttcag gtacagtttt gatcggcgat tgggaaacct
      301 ccaaatattc aaccgccaaa ctggcatccc gtgttggcta tgtatttcaa aaccccgatg
      361 aacaattatt ctctagagac gtcctgaccg aagtggcatt tggtccgcgc aatttggggt
      421 atgcaaaaga aaaagtggac gacctcgtaa agcgcgcttt atccttaacc gagttgagtg
      481 ataagaccga aaccaatcct tacgaccttt cccctacttg gcgcaaaatg gttgctctgg
      541 catccatcat cgctatggat acccccattg tgatcttcga tgaacctacc accgggcaag
      601 atgcagtcaa cgtgggtcgc attgctcatg tcattgcaga actaaagcgt gaaggtaaaa
      661 ctgtcatcac catcacccac gacatcgatt tctgtgcgga gaacttcgac cgtgtcattg
      721 ctctctcaaa gggacaaatc ttactagatg gcatagctcg cgatgtactg ggtcaggaag
      781 aaatcctttc acaaacctat gtcgacccgc cccaactcac ccgcttaggg aaagccctag
      841 ggctcaaaga aacggtaacg aagagagacg aattcttgaa agcaatagaa taaaaaagga
      901 gaggcgacct gcaggtcgcc tctccttttt tattacccat gcaccacttt caatgcatga
      961 ggcagaattt cgaatgccac cttttttaga ttactgccaa aacttgtaaa aatctcacca
     1021 tctgcatgaa tgtaaagcgg agaatcagaa gtgagcgaaa atttcttaaa ttcgcccatt
     1081 tggatctgtt taaaacgcat gtgcgttccg ctcataaact cgggcaccaa tcgaaacatc
     1141 atcgcacgcg aaaccttatt gacaatcaaa aattccattg tgccatcatc gttctttgat
     1201 ttcggcgaaa gcataaaccc accaccctct cgcggaccgt tacacagcgt cagcatcaaa
     1261 gcgcttgcgc tccacgattg atgatcagtt tcaatattca attttgcagg gttatgattc
     1321 aaaagaatgg tttgaattac cgcggtcaag tacatcagaa atcctttgac aatcggcaac
     1381 ttatgcgaac ggatagttac caccgcatca aaaccgatgc caagggtatt atcaaaatat
     1441 tccttgcgcc cgtgttcatc cgtcatcagc cctatatcca caggtttaat attttctgcc
     1501 ttcaaggcat gggcaagcgc atggtctggc ttttggtgga taccaataga atacgcaaaa
     1561 tcatttccag acccaatcgg cacaacgccc atcacagggc gcttctcggc aggcacttgc
     1621 atcaacccgc tcatcacttc atgaaccgta ccatccccgc ccatggcaat caccatgtca
     1681 tatccctctt cggcagcctg ccgcgcaagt tcaatcgcgt gtgttgggta caccgtcccg
     1741 ctccaggtaa gctcaccttg aaattcctga gcaatcggtc gcaacgcatt ggcggtcttc
     1801 cacgcccgcc ccatatctgc catagggttg aggatcaatt tgactttacg aggcatttgt
     1861 ctctccttat tggtttaagg atataacccc accgcctcaa cttcgtaact ttgagttgcg
     1921 tctcgcttca gggaccgtct ttcacttgaa ccggattcaa cctacccgtg atcgccttgg
     1981 caaccacata ctcgccacgg gcatatttct tggcatgata caaagccgag tctgccgcac
     2041 ggatcaagtc acttggggta ttggcatggg tcgggtaaat cgcaatccct gctgtaatgc
     2101 ccaatttcat atccttccct tttggaaatg ggaagtgata atcctgcatg agttcgagaa
     2161 tctttttcat cacagattga gccgcttcgg catctgtatt ccgcatgacc aaagttaact
     2221 cgtcaccgcc ataacgcgca agaaaatctg aactgcgcat tcgctgtgct agaaaattga
     2281 acaaagaata caacagatca tccccaatat tatgaccaaa ggtgtcattt accgctttga
     2341 aaccatccaa gtccatcata acaaccgcaa aggggaactt ggtttgttcg gcaagccgcc
     2401 attcttcttg taagcgttca tccaaggcac ggcggttcgg tagccccgtc acactatccg
     2461 tattcgccat ttgtgccaag cgtttataca tgctagcgtt gaagatggct accgccgcct
     2521 gatctgccaa taagttaata agacgcaatt cagagcgggt aaattttccc actacctttt
     2581 tcgaaaggtt catcaccccc acaacctcat ctttaaattt aagcggcaca ccaataattg
     2641 accctgttaa
//
LOCUS       DAOQFT010000357         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_24635_length_2623_cov_8.314642, whole genome shotgun sequence.
ACCESSION   DAOQFT010000357 DAOQFT010000000
VERSION     DAOQFT010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24635_length_2623_cov_8.314642"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLR93_09300"
     CDS             complement(<1..274)
                     /locus_tag="PLR93_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNH27203.1"
                     /translation="MAKITMREAISQALMEEMDRDPAVFIMGEEVGVWGGTYAVTKGF
                     FDKYGPDRVKDTPIAENAILGGAIGAAMVGQRPIAELMTINFAFSAM"
     gene            complement(353..1309)
                     /gene="pdhA"
                     /locus_tag="PLR93_09305"
     CDS             complement(353..1309)
                     /gene="pdhA"
                     /locus_tag="PLR93_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNH27204.1"
                     /translation="MKKEQLLDLYYQMVLIRRVEERGAELYQAGKIGGFMHLYIGQEA
                     VSTGLIAARESRDRVITAYRDHGVALNCGISANEVMAELLGKATGMSKGKGGSMHMAS
                     TEKNMWGGHAIVGGHLPVAAGLALGDQYAKNDNITICMFGDGATNIGYFHEAVNLAKT
                     WGLRVLWVCENNQYGMGTAVERASAVTEIRQKAEGYGMKNGQVDGMDVMKVYDAAKEA
                     MDFIRKEGQPYFLEIDTYRFRGHSMGDPERYRKSEEVKKWQEEDPIGIFNKYLLDKKV
                     ATRKALDEIEARVEEEVAKAVEFAETSPEPTMEDLYADIYAD"
     gene            complement(1537..2259)
                     /locus_tag="PLR93_09310"
     CDS             complement(1537..2259)
                     /locus_tag="PLR93_09310"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH27205.1"
                     /translation="MKARRDISFAIVVLILSILACNMPSAGSETPSTGEDVSQQPSPG
                     VTYVVVVEDTATIQATETLTPEPPTPQTPPKLTLTKNSNCRLGPSTLYNVVDQIASGA
                     ELSVVGRSEDSEWWQVLNANGRECWVSGENATPNQDFSTLVVGSAPPLPGVPLNFFVA
                     NQICQSAQKKFTVTLSWSSGGGETSFRIYRDGKAIQELKSTRFNFKDVNAPYDKNIVY
                     EIVGLNENGESPRATQIVPACK"
     gene            2341..>2623
                     /locus_tag="PLR93_09315"
     CDS             2341..>2623
                     /locus_tag="PLR93_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27206.1"
                     /translation="MDWFTNPKQNEVKKLITQLADSSRRNAAAQELIRLGADSAAPLI
                     DALQTQDLNLLAIYQHILARIPAASPELTKALKSAHPIIRGRAAEVYGLS"
BASE COUNT          628 a          697 c          608 g          690 t
ORIGIN      
        1 ccattgccga gaaggcgaag ttgatggtca tcaactcagc gatcgggcgc tgaccgacca
       61 tcgccgcgcc aatggcgccg ccaaggatgg cgttttctgc gatgggggtg tctttcacac
      121 ggtcgggtcc gtacttgtca aaaaagcctt tggtaacggc gtacgtgccg ccccaaacac
      181 caacttcctc gcccatgatg aagacggcag ggtcgcggtc catttcttcc attaaggctt
      241 gcgagatcgc ctcgcgcata gttatcttag ccatagtatg ttcgtttcct tgtaaatcga
      301 tattcgttgt tcgattctcg aaagatcgaa gttcgattat cgaatatcgt ttttagtctg
      361 cgtaaatatc cgcgtacagg tcttccatgg ttggttcggg ggaagtctcg gcgaattcca
      421 ctgccttagc tacttcctct tccacgcggg cttcgatctc atcgagcgcc ttgcgagtgg
      481 cgactttctt gtctagcagg tatttgttga agataccgat cgggtcttct tcctgccatt
      541 tctttacttc ttcagacttg cggtaacgct cggggtcacc catggaatgt ccgcggaagc
      601 gataggtgtc aatctcaagg aagtacggct gaccttcttt acggatgaag tccatcgcct
      661 cttttgcggc atcgtagacc ttcatgacgt ccataccgtc gacctgtccg ttcttcatgc
      721 cgtagccttc cgctttttgg cggatctcgg tcacagccga agcacgttca accgcggtac
      781 ccatgccata ttggttgttt tcacaaaccc aaagcacacg taagccccaa gtcttggcga
      841 ggttcacggc ttcgtggaag taaccaatgt tggtggcacc atcaccgaac atacaaatgg
      901 tgatgttatc gttcttggca tattggtcgc cgagggcgag tccagccgcg acgggtaagt
      961 gcccgccgac gatggcgtgt ccgccccaca tgttcttctc tgtgctcgcc atatgcatcg
     1021 agccgccctt gcctttagac atgcccgttg ccttgccgag caactccgcc atcacttcgt
     1081 tggcagagat gccacagttc aacgccacac cgtggtcgcg gtaggcggtg atgacacggt
     1141 cacgtgactc acgcgccgcg atcagaccag tcgaaacggc ttcctgacca atgtacaggt
     1201 gcataaagcc gccgatcttg ccagcctggt acaattccgc gccacgttct tccacacggc
     1261 gaatcagcac catctggtaa tacaaatcaa gcagttgttc tttcttcatt attgggaact
     1321 ccgataagga taatacgcaa aaagcgtaat tttgagttga aaaatactta aggaaagaac
     1381 accaaatttc ctctggcgtt ctgtttccgg taaatcaagt cggggattat atcagagaaa
     1441 gaacctccaa attcaaggtt gataggattt gaaagtgtta aaaaacacaa ctctttgcct
     1501 tagcaaagag ttgtgttttt tatggaaatt tgtgggttat ttacaagctg gcacaatctg
     1561 tgtcgcgcgc ggagattctc cgttttcgtt taatcctaca atctcgtaca caatgttctt
     1621 atcgtacggg gcgttcacat ctttgaaatt gaaacgagtt gactttaatt cctgaattgc
     1681 ttttccatcc cgataaatgc ggaagctcgt ctcgccgccg ccgctgctcc aactcaacgt
     1741 caccgtgaat ttcttctgtg ccgactggca aatctgattg gctacaaaaa agttcagcgg
     1801 cacaccgggc aatggcgggg cgctcccaac cacaagcgtg ctgaaatcct gattgggcgt
     1861 cgcattttcg ccagataccc aacattccct gccattggca ttcaaaactt gccaccactc
     1921 gctatcttca ctgcgcccaa ccaccgaaag ttctgcgcca gaagcaatct gatccacgac
     1981 attatataaa gtactcggac ccaaacggca gtttgaattt ttcgtaagcg tcaatttagg
     2041 cggcgtttgt ggggtaggag gttcaggagt aagtgtttct gtcgcttgaa tagtagccgt
     2101 atcttcaacc accacaacat acgtcacacc ggggctgggc tgctgggata catcttcccc
     2161 cgtgctgggg gtttcactgc ctgcacttgg catattacat gcgagaattg agagaatcaa
     2221 aacaacaatt gcaaaagaaa tatctcttct tgctttcata tggatctcct ctagttggct
     2281 aaagtattct tgtaattatt atacacgccg cccagattca aattcatgta aaataaactt
     2341 atggattggt tcacgaaccc caaacaaaat gaagtgaaaa agttaataac tcaattggca
     2401 gattcaagtc gacgaaatgc cgccgcacaa gaattgatca gacttggcgc tgattctgct
     2461 gcaccgttga ttgacgcgct tcaaacgcaa gacttgaact tgctcgccat ctatcagcat
     2521 attctcgccc gaatcccagc cgcctctccc gaattgacca aagctctcaa gtctgcccat
     2581 cccatcatcc gtggtcgagc ggcagaagta tacgggctga gca
//
LOCUS       DAOQFT010000358         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_24841_length_2609_cov_5.751370, whole genome shotgun sequence.
ACCESSION   DAOQFT010000358 DAOQFT010000000
VERSION     DAOQFT010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24841_length_2609_cov_5.751370"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLR93_09320"
     CDS             complement(<1..832)
                     /locus_tag="PLR93_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH27207.1"
                     /translation="MKLFERIKSIFPARKKAKEEEQPQAVSAVNEPSTSPRKEVKKAD
                     RFTIVSWSVTSVLVVALLSGTLYYKSTLPATVVTVPAATEEPAADGSVPVVVNSGTGG
                     GGSEGLFPTILRKLQIKTNKPERPRYEPELYRVVRGDSMYRIAEEFKVKSETILYVND
                     QLEDNPHSLRPGMELTIPPADGLYYTWKEGDTFESVAEEFDANADDIINFTGNQIDLT
                     DPKVEVGTQIFIPGGSRELVNWAAQLPTNARGVNTGTGGGNAANVCGGGPVASGFGWP
                     A"
     gene            complement(879..2156)
                     /gene="rho"
                     /locus_tag="PLR93_09325"
     CDS             complement(879..2156)
                     /gene="rho"
                     /locus_tag="PLR93_09325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNH27208.1"
                     /translation="MKILELENEPLSKLRMMARDLNIQNANRLKKETLAMLIREAEAQ
                     KEGIELRGGILEIMSEGIGFLRATNYRISDQDVYVSQAQLRRYDLRAGDLVIGQVRPP
                     RESERHFGLLKVESINGLDPEDAIRRTVFENLTPIFPDKRFDLETDPKTLAPRLINLI
                     APIGRGQRGLIVSPPKTGKTTILKQIANSISTKYPDVHLIIALIGERPEEVTDMDRSV
                     EAEVVASTFDEPVTSHVRTAELALDRAKRLVEIGRHVVILMDSITRLARAYNLTVNPS
                     GRTLSGGMDPSALYPPKRFFGAARNVEEGGSLTIIATCLVDTGSRLDDVVYEEFKGTG
                     NMELHLSRKLQERRTFPAVDLERSSTRREDLLLGPDLLQRVWLLRRMFVQMTSSQPAG
                     AGMDPSVATEAILTRLEKSKNNIEFLENLTKDA"
BASE COUNT          622 a          590 c          680 g          717 t
ORIGIN      
        1 cggcgggcca accaaagccg cttgcaacag gtccaccgcc acaaacattg gctgcgtttc
       61 caccgcctgt gccggtgtta acgccacgag cattggtagg caattgcgct gcccagttta
      121 ccaactcgcg agaaccaccg ggaataaaaa tttgggtgcc aacttccact ttggggtcgg
      181 tcaggtcgat ttgattgcca gtgaagttga taatgtcatc ggcattggca tcaaattctt
      241 cagccaccga ttcaaaggta tcgccttctt tccaggtgta atacaaacca tctgcagggg
      301 gaatggtcaa ctccatgccg gggcgcaaac tatgtgggtt atcttccaac tggtcgttga
      361 catataaaat ggtttcagac ttaactttga actcttctgc gatgcgatac atcgagtcgc
      421 cgcgcacaac acgataaagt tcaggttcat agcgcggacg ttccggcttg ttggtcttga
      481 tctgcaattt gcggagaata gtcgggaaga gaccttcgct tcctcctccg cctgtacctg
      541 agttgaccac taccggtaca ctgccatctg ccgccggttc ttccgtcgcc gcagggacgg
      601 tcaccacagt agcgggaagg gtgcttttgt aatagagcgt tccactgagc aatgccacaa
      661 ccaagaccga agtcacagac caactgacga tggtaaagcg atccgctttc tttacttcct
      721 tacgcggact ggttgagggt tcattcactg ctgaaacagc ttgaggctgc tcttcttctt
      781 tggctttctt gcgtgccggg aagatggatt tgattctttc aaaaagtttc atttttaatg
      841 ggtgttacga ttttgtacca attcaacttt tgttttcgtt atgcgtcttt tgtaagattt
      901 tccaaaaatt cgatgttgtt ctttgatttt tccaagcgcg tcaaaatggc ttcggtcgcc
      961 acagaaggat ccataccggc gccagcgggc tgggatgaag tcatctgtac gaacatgcgg
     1021 cgtaggagcc aaacacgctg gagcaggtcg ggtccgagca gcaggtcttc gcggcgggtg
     1081 gacgaacgtt cgaggtctac ggcagggaag gtacggcgtt cctgcaattt gcgcgagagg
     1141 tgcaactcca tgttaccggt gcctttgaac tcttcgtaca ccacatcatc gagacgagaa
     1201 ccggtgtcaa cgagacaggt tgcgatgatg gttagcgagc cgccttcttc cacgttacga
     1261 gccgcaccga agaaacgctt gggcgggtac aacgcggagg ggtccatacc gccggagagg
     1321 gtgcgcccag aggggttgac ggtcaagttg taggcgcggg cgaggcgggt aatggagtcc
     1381 attaagatta ctacatgtcg accgatctcg accaatctct tagcgcggtc taatgcaagt
     1441 tcggcggtgc gaacgtgcga agtgaccggc tcatcgaagg tggaggcgac cacttcggct
     1501 tccactgagc ggtccatgtc ggtaacttct tcagggcgtt cgccgatgag tgcaataatt
     1561 aagtgaacgt ctggatattt tgtagaaatt gaatttgcaa tttgcttcaa aattgtagtt
     1621 ttccccgttt tgggcggcga aacgatcaaa ccgcgctgcc ctctaccaat aggggcaatt
     1681 aagttaatta aacgtggagc taatgttttg gggtctgttt ctaaatcaaa acgcttatct
     1741 ggaaagattg gggtgagatt ttcaaaaacc gttctacgaa tggcatcttc cgggtctaag
     1801 ccattgatgg attctacttt taataaaccg aagtgtcttt cactttctct gggagggcga
     1861 acctgcccga tcaccaaatc ccccgcacga agatcataac gtcttaattg agcttgcgaa
     1921 acataaacat cttggtcgct tattctatag tttgtggctc gcagaaaacc gatgccttcg
     1981 ctcataattt cgagaatacc gccgcgaagt tcaatacctt ccttctgtgc ctctgcctcg
     2041 cggatgagca tggcgagagt ttctttcttt aggcgattgg cattctggat attcagatcc
     2101 cttgccatca tacgaagttt ggaaagaggc tcattttcca gttcaagtat tttcattttc
     2161 ttctctctcg acatatatga attttcagag gcaggggtat tgggtacgct aggcggaacg
     2221 gctggcgtgt ttgtagattc aaccaccggg gcaggcgggg tatttgaatc aaaagaatta
     2281 tttctgttat tgggtttggg cgaacgttgg tagggtcttc ttgtagagtt ggttgaacga
     2341 tttgacgtat ttgtaggttt atttggcatg tgagttgatc tgttgggatg agaatgtgaa
     2401 aagaccagtc cccgcttggg ctggttaatt cctaaaaggt tttaatacaa tgtgggatgg
     2461 agttcaaaga tataggattc aacttgcaga cgtagatata ttgtgaaata tttttactgc
     2521 gattttttta aaacggcagg ggggaacaat cgaattatag cacttccata caacaggtgc
     2581 aagcaatcaa aaaatcaatt ttcgtctgc
//
LOCUS       DAOQFT010000359         2604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_24940_length_2604_cov_4.121224, whole genome shotgun sequence.
ACCESSION   DAOQFT010000359 DAOQFT010000000
VERSION     DAOQFT010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24940_length_2604_cov_4.121224"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLR93_09330"
     CDS             complement(<1..941)
                     /locus_tag="PLR93_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNH27209.1"
                     /translation="MAFNPINWLLGLFSLDIAIDLGTANTLVHVRGKGIVINEPSWVA
                     VDKKQRQPLAIGLEAKEMVGRTPANVVVVRPIRDGVIAEFDVTRVMLEYFIGKVHEQS
                     VVPLPRPRVIVGLPTGVTEVEKRAVYDAVMASGARQAMLIEEPIAAAIGAGLPIGEVR
                     GSMVVDIGGGTTEVAVMSTGGVVASRSLRVAGDEMDQDVVQYLRNKYNLLVGTGIAEQ
                     IKWQIGSAYPLQPEKTMEVRGRNLVTGLPETVEISSVEIREALSGSVQVIIDTVRDAL
                     DEIPPEIVSDLMDIGICLAGGGALLQGLAERLTDELK"
     gene            1081..1932
                     /locus_tag="PLR93_09335"
     CDS             1081..1932
                     /locus_tag="PLR93_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNH27210.1"
                     /translation="MSKFAIVTDSTSYIPTDVAQKHGITIAPQVLIWDNQTYRDGIDI
                     QPTDFYSRLKTAKSMPSTSQVSPATMQEIFQGLVEKGTSVLGIFISSKLSGTLQSAIQ
                     AKDMMGSAGEKVTLVDSRSTAMGLGFQAIIAARAAEAGASIEEAASQASSAHERTGVF
                     FAVDTLEFLHRGGRIGGAQRFIGSALNLKPILAVKEGKVEGIERIRTKSKAHERVLEL
                     VAEQVKGKSNVRIATLHANSADDAKSLLDRAATELSPVETLFTEVSPVVGTHAGPGTV
                     GLAYIHD"
     gene            1936..>2604
                     /locus_tag="PLR93_09340"
     CDS             1936..>2604
                     /locus_tag="PLR93_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH27211.1"
                     /translation="MIDPKQFERIVQSMPFLKNADSGFVKEFQKSAFLAQIPAGHDVF
                     LEGDRVEATALLISGVVRVYKIGETGREITLYRFGNGSSCILTANAILSRNTFPAIAT
                     VEQDAEAVMIPADVFRDWVKRYDLWRDFVFDLLSQRLSTVMAVVDEVVFHRMDRRVAN
                     LLLKRGKEENPIHITHQEIASDLGSSREVISRLLEDFISEGSVRSGRGIIEVLDFEAL
                     EDRSL"
BASE COUNT          700 a          655 c          596 g          653 t
ORIGIN      
        1 aatttcagtt catcggttaa acgttctgcc aagccttgca agagcgcgcc gccgccggct
       61 aaacaaatac caatatccat taagtctgaa acgatttcgg gcggaatttc atccaaggca
      121 tcgcgaacgg tatcaatgat cacttgcacc gaaccagaga gcgcttcgcg aatttcaaca
      181 gaagaaattt caaccgtttc aggtaaacca gtcactaaat ttctgccgcg cacttccatt
      241 gttttttcag gttgcaatgg atatgccgac ccaatttgcc atttgatctg ttcagcaatc
      301 cccgtcccca ctaataaatt gtatttgttg cgcaaatact gcacaacatc ttgatccatt
      361 tcatcaccag ccacgcgcaa tgaacgtgaa gccactacac cacccgtaga catgactgcg
      421 acttcagttg tgccgccacc aatgtcaacg accatggaac cgcggacttc tccaattgga
      481 agaccggctc cgatcgccgc cgcaattggt tcttcaatca acatagcttg gcgcgcgcct
      541 gatgccatta ctgcatcata cacagcgcgc ttttccactt cggtcacacc ggtaggtaaa
      601 ccaacgatca cacgcggacg tggcaaaggc acaacactct gttcatgtac ctttccaata
      661 aaatactcaa gcatcacacg tgttacatcg aattctgcaa tcaccccatc acgaatagga
      721 cgaaccacca ccacattggc gggagtacgc cctaccattt ccttggcttc aagaccgatc
      781 gcaagaggtt ggcgttgttt cttatcaacg gctacccaag acggttcatt gatcacaata
      841 cccttaccac gaacatggac aagagtattt gcggtgccaa ggtcaatggc aatatccaaa
      901 gaaaaaaggc ctaacagcca gttaattggg ttgaaggcca aaatcggctc ctatgatacg
      961 agaatgggtc taagcaaaac tgccgagcca ttataccatg aggatgagtc cgaatatttt
     1021 ttaattctgt tacccaaacg gggtaaaatc aggaaaattt ttccccgtgg aggaagagaa
     1081 atgtcaaagt ttgccattgt taccgatagc acttcttata ttccaaccga tgttgcccag
     1141 aaacatggaa ttaccattgc cccacaagtt ctcatttggg acaaccaaac ataccgcgac
     1201 ggtattgata ttcagcccac cgacttctac agccgcctca agaccgccaa gtcgatgccg
     1261 tcaacttcgc aggtttctcc tgccactatg caggagatat ttcaggggtt ggtcgaaaaa
     1321 ggaacttctg tgcttggcat cttcatctct tcaaaattat caggcacatt gcaatctgcc
     1381 attcaagcca aggatatgat gggaagcgct ggcgaaaaag taaccttagt tgacagccgc
     1441 tctactgcaa tgggacttgg gtttcaggca attattgctg ctcgcgccgc agaagctggc
     1501 gcaagcattg aagaagctgc gtctcaggca tctagcgcac atgagcgcac aggagtgttc
     1561 tttgcagtag atacacttga attcttacac cgtggcgggc gtattggagg cgcacaacgc
     1621 tttatcggct ctgcgctgaa cctcaagccg atccttgcag tcaaagaagg taaagtagaa
     1681 ggaatcgaac gtattcgcac caagagcaag gctcatgaac gtgtgcttga attggttgcc
     1741 gaacaggtca aaggaaaatc aaatgttcgc attgcgactc tccatgcgaa ctcggcagac
     1801 gatgccaaaa gcttgttaga ccgcgctgcg actgaactct ctccggtgga aaccctattt
     1861 accgaagtta gcccggtggt tggaacccat gccggacctg gaaccgtagg gctggcttac
     1921 attcacgatt aatccatgat cgacccaaag cagtttgaac gtattgttca atctatgcct
     1981 ttcttgaaga acgcagactc gggatttgta aaagaattcc aaaagtctgc gtttcttgcg
     2041 cagattccgg cagggcatga tgtctttctt gaaggggatc gtgttgaagc cactgccttg
     2101 ctgatctctg gcgtggtacg tgtttataaa attggtgaaa cagggcgcga gattacgctc
     2161 tatcgctttg gtaacggttc atcctgtatt ctgaccgcga atgcaatctt gagccgaaat
     2221 acatttccag cgattgccac agttgagcaa gatgcagaag ctgtgatgat tccagccgat
     2281 gtatttcgag attgggtcaa gcgctatgac ttgtggcgtg actttgtttt tgacctgtta
     2341 tctcaacgcc tttctacagt catggctgta gttgacgaag ttgttttcca ccgcatggac
     2401 cgccgagttg ccaatttact cctgaagcgt ggaaaagaag aaaaccctat tcacatcact
     2461 caccaagaaa tcgcttcaga tctgggaagt tccagagaag tgatcagccg cttacttgaa
     2521 gatttcatca gtgagggcag tgtccgttcg gggcgtggaa tcatcgaagt tctagatttt
     2581 gaggcgttgg aagatcgttc gctt
//
LOCUS       DAOQFT010000360         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_24982_length_2602_cov_3.137024, whole genome shotgun sequence.
ACCESSION   DAOQFT010000360 DAOQFT010000000
VERSION     DAOQFT010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24982_length_2602_cov_3.137024"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLR93_09345"
     CDS             <1..1042
                     /locus_tag="PLR93_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNH27212.1"
                     /translation="AVEPFLYAALLLEIAAMLVVPLLVPLYQRPGRGVTRFIIYQTLA
                     MPFILFSGWMLAGVEASPGDLGTIVQSGVMVTMGFAFLFAVFPLYNWIPQLMEEATPY
                     AVGFLLWALPTFTTIFALGFLDRYTWLRNTPQIANAILFSGVIMTATGGIFGALQRHL
                     GRIFGYAAIAETGLLLIALGLRSSEVVNIAFLILISRGLELSVWALALSIIKRNAYPL
                     MFSDVQGLARKYLVATSALIIAHLSVAGFPLLAGFPSRLALWQELSLQSLSASLWVFL
                     GLLGLLVASVRTLATLVMAGDDAPWELNETWVQTIMLSAGVFGIFLLGLFPQILQPFL
                     SSLPALFEHLIQ"
     gene            1086..2276
                     /locus_tag="PLR93_09350"
     CDS             1086..2276
                     /locus_tag="PLR93_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27213.1"
                     /translation="MEFEQIVKRLEFLDKQQRETKETLTSLKESLASFETTVNAVSKQ
                     IKTLSKQVTDITPAAKRVDQFETMVGKQRNELVKIIEDIDKAHTRSERDTAKQFQSEI
                     NELSKSLTQLKISTDLTEIKKQLKERGNEIQRALANIADFKTVVEDTKRSNEDVKLSI
                     KAADEARKNDLKRVADLQGELTALRKRIDENREKSTIQGDTIRNIETRITELLSSELE
                     RKQAQAVFLDQQRVAQVDRERAWKDWQDKFVSFQKEAETLDQQIQRLDETLRNAKKAQ
                     DTYMELNSKLERRINEVTEMQRLAEERLRQEWISFKADDQKRWTGYSLSSEESFRDIR
                     KEVSKYEGSISTLNEVSQVLQDQLHQTTDASEKQLQEMMNVVHEWMTSYQRIMGHGKK
                     PTKK"
     gene            2354..>2602
                     /locus_tag="PLR93_09355"
     CDS             2354..>2602
                     /locus_tag="PLR93_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH27214.1"
                     /translation="MRVIGTAGHVDHGKSTLIETLTGTHPDRLKEEKAREMTIELGFG
                     WLTLPNGEEIGIVDVPGHRDFIENMLSGIGGIDAVLLVI"
BASE COUNT          732 a          622 c          580 g          668 t
ORIGIN      
        1 cgcagtggaa ccgttcttgt atgctgcact cttgctggaa atagcagcta tgttggttgt
       61 accattgctt gttccactgt accagcgacc gggtcgaggc gtgacgcgct tcatcatcta
      121 ccagaccctc gccatgccat tcatcctctt ttctggctgg atgctggcag gcgttgaagc
      181 gagtccaggt gacttgggga cgatcgttca atcgggtgtg atggtcacaa tgggctttgc
      241 ttttctcttt gccgtcttcc ccctctacaa ttggattcca caattgatgg aagaagctac
      301 tccatatgca gttggttttt tgctttgggc attgccaacc tttacaacca tctttgcctt
      361 aggcttccta gaccgctaca cctggttacg caatacgcca caaatcgcaa acgccattct
      421 tttctcaggt gttatcatga ccgccacagg cggaattttc ggcgcattgc aacgtcacct
      481 tgggcgtatc tttggatacg ccgccattgc tgaaaccggt ttgttattga tcgcactcgg
      541 tttacgttca tctgaagtcg tcaatatagc cttcctcatc ctcatatctc gcggactaga
      601 actaagcgtt tgggcgttgg cactatctat tattaaacga aatgcttatc cacttatgtt
      661 cagtgatgtt caagggttgg cgcgaaaata ccttgtggca acgagtgcat tgattattgc
      721 gcatctctct gtcgctgggt ttccactttt ggcgggcttc ccctctcggc tggcattatg
      781 gcaagagtta tcgctccaat cgctgagtgc atccctatgg gtcttccttg gtttattggg
      841 attgttagtc gcctcggttc gtacattagc cactcttgtg atggcaggcg atgatgcgcc
      901 gtgggagcta aatgagacct gggttcagac cattatgctg agtgcgggtg tatttggcat
      961 tttcctgcta ggactctttc ctcagatcct gcagccgttc ctttccagtt tgcctgcttt
     1021 gttcgaacac cttattcaat aatcccgttt tgcaaaaggg tataatttaa cgaaacggag
     1081 tcctcatgga atttgaacag atcgtaaaaa gacttgaatt tttagataaa caacaacgcg
     1141 agactaagga aactcttacc tcgttaaaag aaagccttgc ctcctttgaa actacagtaa
     1201 atgctgtttc aaagcagata aaaaccctta gcaagcaggt cacagatatt acgcctgccg
     1261 ccaagcgtgt tgaccaattc gagaccatgg ttggaaaaca gcgtaatgag ttggtcaaaa
     1321 taatcgaaga catagacaag gctcatacac gctcagagcg cgacaccgcc aaacaatttc
     1381 agtctgaaat aaacgagctc agtaaatcac taacacagtt aaaaatcagc actgacctca
     1441 cagagatcaa aaaacaactc aaagaacgtg gcaatgaaat tcaacgtgct ttggcaaata
     1501 ttgcagactt caaaacagtt gtggaagaca ccaaacgctc gaacgaagat gtaaagttat
     1561 ccattaaagc ggcggatgaa gcccgcaaaa atgacctcaa acgcgttgca gacttgcaag
     1621 gtgagctcac agcgctgcgc aaacgcatag atgaaaaccg tgaaaagtcc acgattcaag
     1681 gcgataccat tcgcaacatt gaaactcgta tcactgaatt actctcttca gagttggaac
     1741 gtaaacaagc ccaagctgta ttcttagatc aacaacgcgt tgcccaagtg gaccgtgaac
     1801 gcgcctggaa agattggcaa gacaaatttg tttctttcca aaaagaagca gagaccttgg
     1861 accaacaaat tcaacgtttg gatgaaacct tacgcaacgc caagaaagcg caagacactt
     1921 acatggaact caattccaag ttggagcgcc gcatcaacga agtgactgaa atgcagcgtc
     1981 ttgccgaaga acgcttgcgt caggaatgga tcagcttcaa agccgatgac caaaaacgtt
     2041 ggaccggata cagcttatcg tctgaagaat ccttccgcga tattcgcaaa gaagtgagca
     2101 aatacgaagg cagtatttca actcttaacg aagtatcgca agttctccaa gatcaactgc
     2161 accaaaccac cgatgcttct gaaaaacaat tacaagagat gatgaacgtt gtccatgaat
     2221 ggatgacttc ataccaacgc attatgggac atggcaaaaa gccaacgaaa aaataaaaaa
     2281 gtccttccaa agtggaagtc gaaagatttc cactttttat ttggtttatt caaattatgg
     2341 ttaaattgag atcatgagag tcattggcac agcaggtcac gttgaccatg gaaaatccac
     2401 gcttattgaa acgttgacgg gcacacaccc agaccgtctc aaagaagaga aagctcgtga
     2461 aatgaccatc gagcttggct ttggctggct taccctgcca aacggcgaag agatcggcat
     2521 tgtcgatgtc cctggtcacc gtgactttat cgaaaatatg ctctccggca tcggcggcat
     2581 tgacgcggtt ctgctcgtca tc
//
LOCUS       DAOQFT010000361         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_24994_length_2601_cov_5.318146, whole genome shotgun sequence.
ACCESSION   DAOQFT010000361 DAOQFT010000000
VERSION     DAOQFT010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24994_length_2601_cov_5.318146"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLR93_09360"
     CDS             complement(<1..407)
                     /locus_tag="PLR93_09360"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNH27215.1"
                     /translation="MTEDKSSRLPYRLREPFLSNTEAALFRLLTDLMGDRYMICPKVA
                     LTDLFMIVRPNENVHFFNKIFRKHVDFLLCDPKTFTPTFGVEIVKSIAKDGMREADKF
                     LAELFTDAGIPLVHIPTNDKYEPADLIALFQLAV"
     gene            complement(453..1217)
                     /locus_tag="PLR93_09365"
     CDS             complement(453..1217)
                     /locus_tag="PLR93_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNH27216.1"
                     /translation="MNLQPYLEFATSLAYHAGKITLRYFNTGIVPDRKENNDPVTIAD
                     REAEAFVRSQVEKHYPGHAIVGEEYGEQGTGHSFRWIVDPIDGTISFVQGVPLYGVLI
                     ALEIDGVVQVGAAYFPPLDEMLTAGSGLGCWWNGRRARVSGVSKLSEACVVTSDFQHL
                     EKKQAGITKRFEESGALLRTWGDAYGYLLVATGRAEVALDPRMDVYDCGPYPVIMKEA
                     GGYFGSWIGEEGHDHGEGLACNAALKPDVMKLMRGK"
     gene            complement(1214..2455)
                     /locus_tag="PLR93_09370"
     CDS             complement(1214..2455)
                     /locus_tag="PLR93_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27217.1"
                     /translation="MQKDDVLRLLRDPNLIHPEKLTLLSNIADLAASLDMPCYVVGGF
                     VRDLLLQKPVNDLDVIVEGDAIKLGKKLVEAYGGKLTTHFKFNTAIWYLPNSTDFVDL
                     ITARKETYEKPGALPTVTPSTMEDDLRRRDFTINAMALRLDGEGFGEILDPLKGQTDL
                     ERGVIRVLHPNSFIDDPTRIFRAIRYEARYGFSMDEETSGLVNTEALKVLHSLSGERI
                     RHELDLILDEDESSKMMLRIVNLGVLSAIHHKMPEFNQDYADFLEMDVRLDVQADRRT
                     MGYILWFMDLSEEDVILLANRLDFTNELTLAVWAGAQLKRSLIHLVGARPSEWTYALE
                     KLPLLSIYVVYLVSRENDLLSYISLWRHVKPQTTGNDLKAKGLLPGPRFSEILLRLRS
                     AWLDGEVNNESQEKELLAKLL"
BASE COUNT          684 a          695 c          579 g          643 t
ORIGIN      
        1 actgccagtt ggaacaatgc aatcaaatct gctggttcat atttatcgtt cgtaggaatg
       61 tgaaccagcg gaattccagc atcagtaaaa agttccgcta aaaacttatc ggcttcacgc
      121 ataccatctt ttgcaatcga cttgacgatc tccaccccaa aggtgggagt gaaggtcttc
      181 gggtcgcaca atagaaaatc aacatgcttg cggaaaatct tattaaaaaa gtgaacgttt
      241 tcgttcgggc gtacgatcat gaacaagtct gtcagagcaa ccttagggca aatcatataa
      301 cgatcaccca tcagatccgt caacaaacga aaaagagccg cttccgtatt gcttaggaag
      361 ggctctcgaa gacggtaagg aagtcggctg cttttatcct cggtcataat tcgattatac
      421 gttaggtttg aatctacgta cagaaatttg atctactttc cgcgcatcaa tttcataaca
      481 tctggcttga gtgcggcgtt acaagccaac ccttcgccgt ggtcatgacc ttcttcgcca
      541 atccacgaac cgaaataacc acctgcttct ttcataatca ccgggtacgg accgcaatca
      601 tacacatcca tgcgtgggtc tagtgccact tctgcacgcc cagtagcgac caataaatat
      661 ccataagcat caccccacgt ccgcagcaga gcgccgcttt cttcaaaacg ttttgtaatg
      721 cccgcctgct tcttttcaag gtgctgaaaa tctgaagtga ccacacaggc ttctgaaagt
      781 ttactcacgc ctgaaacacg tgcgcggcgt ccattccacc aacagcccag cccgctgccc
      841 gctgtcaaca tctcatccag cggcgggaag taagcagctc ctacctgcac cacaccatca
      901 atctccagag cgatcaatac cccatataaa ggaacaccct gcacaaacga gatcgtccca
      961 tcaatggggt ctacgatcca gcggaatgag tgacccgttc cttgctcgcc atattcctcc
     1021 cccacaatgg catgacccgg ataatgcttt tccacctgcg agcgcacgaa agcttcggct
     1081 tcgcggtcag caatggtcac agggtcattg ttttctttac ggtctggcac aatgcctgtg
     1141 ttgaagtacc ttaaagtaat cttccccgcg tgatacgcaa gggaagtggc gaattcgaga
     1201 tagggctgaa ggttcataga agtttggcaa gcaattcctt ttcttgagat tcattgttca
     1261 cttccccatc caaccaggca gagcgcaaac gtaataagat ctcgctaaaa cgcggacccg
     1321 gcaacaagcc ttttgccttg agatcattcc ccgtagtctg tggtttgaca tgccgccaga
     1381 gtgagatata actcaacaaa tcattctcac gtgagaccag atacaccaca taaatagaaa
     1441 gtagcggcag cttttctaaa gcataggtcc attcactggg gcgcgcgcca acaagatgga
     1501 tcaagctacg tttcaattgt gcccccgccc aaaccgcgag cgtcagttca ttcgtaaaat
     1561 ccaagcggtt cgcaagcaag atcacatctt cttccgataa atccataaac cataagatgt
     1621 agcccattgt gcgccggtcg gcttgtacat ctaaacgaac atccatctca agaaaatcag
     1681 cataatcttg attgaactct ggcatcttgt ggtgaatagc actcaacacg cccaagttga
     1741 ctatgcgcag catcattttt gaggattcat cttcatccag aatcaaatcc aattcatggc
     1801 ggatgcgttc tccgctaagc gaatgcaaaa ccttcaacgc ctccgtattg actaagccag
     1861 aggtttcctc atccatcgaa aatccatagc gtgcttcgta gcgaatggcg cgaaagatgc
     1921 gggttgggtc atcaataaac gaattcggat gcaaaacgcg gatcactccc cgctcgaggt
     1981 ctgtttgccc tttcagcggg tcgaggattt cgccgaaacc ttcaccgtcc aaacgcagtg
     2041 ccatagcgtt gatagtaaag tcgcggcggc gtaaatcatc ttccatcgtt gacggtgtga
     2101 ccgttggcaa tgcgccgggc ttttcgtagg tttctttgcg agccgtgata aggtcgacaa
     2161 aatcggttga gtttggcaaa taccagatcg cggtattgaa tttgaagtgt gttgtgagct
     2221 tcccaccata cgcttcgacc agttttttcc caagtttgat ggcatcgcct tcgacgataa
     2281 catccaaatc gttcactggt ttttgaagca gtaagtctcg gacgaagccg cccaccacat
     2341 agcacggcat gtccaaggat gcggctaggt cggcgatgtt cgagagaagg gtcagcttct
     2401 cggggtggat caggttcggg tcccgtaaga ggcgcagcac gtcatccttt tgcataaatc
     2461 taattttacc cttataatca gagcatgttg atgaacccaa acccgtcccc tcgaagttgc
     2521 acatcctcca ggtctggcta ggttcactta agtgcgccca aacggctccc agacttggtg
     2581 agccgttttt tattggatgg t
//
LOCUS       DAOQFT010000362         2593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25123_length_2593_cov_4.448779, whole genome shotgun sequence.
ACCESSION   DAOQFT010000362 DAOQFT010000000
VERSION     DAOQFT010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25123_length_2593_cov_4.448779"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLR93_09375"
     CDS             complement(<1..778)
                     /locus_tag="PLR93_09375"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNH27218.1"
                     /translation="MNLDDLELFKQIDRQNMRAQIDGLPDQLQSAWELAQTLPLPKFD
                     DIQNIILSAMGDSGSATELVVASVFSSLSLPVSVHRGYGLPAYATKKTLVVCLSHSGN
                     SEEVLDVFESAQKNGCQLMVISTGGELTKRAQANNLPFWNFTYAGMDTTAIAYPFALT
                     LALFARLGLIPDPSADVAEAVAMMKRSQQHIVPEVIAAKNPAKRYAGQLVGRWVTFVG
                     TEQLAPVAYRWKTQINQLAKAGANFEIIPEATHNTLIATIN"
     gene            complement(851..2323)
                     /locus_tag="PLR93_09380"
     CDS             complement(851..2323)
                     /locus_tag="PLR93_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27219.1"
                     /translation="MAEEKKKDEEKKEEKATPKDNIVTTKHSVKIGGKTLKYTVTTGT
                     MVLKEEVNEKDGEIEKARAQIFFTAYTLDGVRDKAKRPLTFSFNGGPGSSSVWLHLGV
                     LGPRRVVMTNEGEMPKPPFKLTDNEFSVFDETDLVFIDPMSTGFSRPVEGEKSKDFHT
                     FHKDIASVGDFIRLYTTRYNRWLSPKFLAGESYGTTRAAGLSGYLADRHGMLLNGLML
                     ISSVLDFTTLDFNLNNDLPYILFVPGYTATAWYHGKLDKKIPLKTWLKESEEFALGEY
                     TAALMKDGALTKEERRKVAEKLSRLTGISVDFIERGNLRIHTFHFFKELLRDEGKTVG
                     RLDSRYTGIDRLGVTETFEYDPLFAQVNGPYTAAFNDYIRSELKFETDTTYEILSDKV
                     WRQWSYSYFENQYVNVAETLRASMTFNKYLKVFVANGYYDLGTPYFATEYTFNHLALD
                     ESLRKNIRMGYYEAGHMMYVHMPSLKQMKKDLSKFIKDAS"
     gene            complement(2335..>2593)
                     /locus_tag="PLR93_09385"
     CDS             complement(2335..>2593)
                     /locus_tag="PLR93_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27220.1"
                     /translation="GFPSGRMSTQAKAAEIAWMGVSAPSHRRMRHLYDVIGKQLAKHI
                     QNPILADVAFFLLIPVEWISFFVLQLIVPEIQFMSERLYRS"
BASE COUNT          586 a          598 c          727 g          682 t
ORIGIN      
        1 ggttgatggt ggcgatcagc gtgttgtgcg tcgcttcggg gatgatctcg aagtttgccc
       61 cagccttggc aagctggttg atctgagtct tccaccgata tgccacgggg gcgagttgtt
      121 ctgtgccaac aaaagtgacc caacgtccca ctaattgacc tgcataacgc ttggcggggt
      181 tcttcgccgc gatcacctca gggacaatgt gctgctgtga gcgcttcatc atggcgacgg
      241 cttcggcaac atctgcggag gggtcgggaa tcaagccgag gcgggcgaag agagcgaggg
      301 tcaacgcgaa gggataggcg atggcggtcg tgtccatgcc tgcgtaggtg aagttccaaa
      361 aagggaggtt gttggcttgc gcacgtttgg tcaactcgcc gccggtggag atgaccatca
      421 attggcatcc gtttttctgg gcagactcaa aaacatctaa tacttcttct gagttaccag
      481 aatgtgagag gcatacgact aatgttttct ttgtggcgta tgcgggtaag ccatagccgc
      541 gatggacgct cacgggtagt gaaaggcttg agaaaacaga ggcaactacc agttcggtag
      601 cggagccgga gtcgcccatg gcggatagga tgatgttttg gatgtcatca aatttgggca
      661 atggcagtgt ttgtgccaat tcccatgcgc tttgcagttg gtcgggcaag ccgtcaattt
      721 gggcgcgcat gttttggcgg tcaatttgtt tgaagagttc gaggtcgtct aagttcatgg
      781 tttatacaca atgagacctg tcaggttttt acgaagtccc cggaggggga aacctgacag
      841 gtctttttag ttaactggca tccttaataa acttgctcaa atccttcttc atctgcttga
      901 gcgaaggcat gtggacgtac atcatgtgtc cagcttcgta atagcccatg cgaatgttct
      961 ttcgcaaaga ctcgtcgagc gcgagatggt tgaaggtgta ttcggtggcg aagtaaggcg
     1021 tgccaagatc gtagtagccg ttggcaacga agaccttgag atatttgttg aaggtcattg
     1081 aagcccgcaa agtttcagcc acattgacat attgattttc aaaatagctg tatgaccatt
     1141 gccgccaaac tttatcgctc aaaatttcgt aggtggtatc ggtttcaaat ttgagctccg
     1201 agcggatgta atcattgaac gccgccgtgt atggaccgtt gacctgtgca aagagcggat
     1261 catactcaaa agtctcagtc acgccgaggc ggtcgatgcc ggtgtaacgg ctgtcaagcc
     1321 gaccgaccgt tttgccttca tcacgtaaaa gctctttgaa gaagtggaag gtgtgaatgc
     1381 gcaaattacc gcgttcgata aagtccaccg agatgccggt gaggcgcgaa agtttttctg
     1441 caacctttct tctttcttct ttggtgaggg cgccatcctt catcaaggcc gccgtgtatt
     1501 cgcccaacgc aaattcttct gattctttga gccaggtctt gagcggaatc tttttgtcga
     1561 gtttgccgtg ataccaggct gtagccgtgt acccaggtac aaaaagaata taaggcaggt
     1621 cattgttgag gttgaagtcg agcgtggtga agtcgagcac cgatgagatg agcatcaacc
     1681 cgttgagcaa catgccgtga cgatcggcaa ggtagccgga gagtcctgcc gcgcgtgtgg
     1741 tgccatagga ttcgcctgcc aggaacttgg gcgaaagcca gcgattgtag cgcgtggtgt
     1801 agagacgaat aaaatcaccg accgatgcga tgtccttgtg gaacgtatga aaatctttag
     1861 acttctcgcc ttccaccggg cggctgaagc cagtgctcat cgggtcgata aagacgaggt
     1921 cggtttcgtc gaagacagaa aactcgttat ccgttaattt gaagggcggc ttgggcatct
     1981 cgccttcgtt ggtcatcacc acgcggcgcg gaccgagcac accgagatgc agccatacgg
     2041 aggatgagcc aggtccgccg ttgaacgaga aggtaagcgg gcgtttggct ttatcacgca
     2101 caccatcaag cgtgtaggca gtgaaaaaga tttgcgcgcg tgctttttct atttcgccat
     2161 ctttttcatt cacttcttct ttcaacacca tcgtgccggt tgtgacggtg tacttgagcg
     2221 tcttgccgcc gatcttcacc gaatgtttgg tggtgacgat attgtccttg ggggttgctt
     2281 tttcttcttt cttttcttcg tctttctttt tttcttcagc catgtgagtc tcctttatga
     2341 acgatataaa cgttcggaca tgaattgaat ttcagggacg attaattgta gtacaaagaa
     2401 cgaaatccat tctacgggaa taagaaggaa aaaggcaacg tctgcaagaa tgggattttg
     2461 gatatgcttc gccaactgct tgccgatgac gtcatatagg tgccgcatcc ttctatgact
     2521 tggggctgat acgcccatcc aggcgatttc cgccgccttg gcctgcgtgc tcatccgccc
     2581 gctcggaaat ccg
//
LOCUS       DAOQFT010000363         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25206_length_2587_cov_5.785150, whole genome shotgun sequence.
ACCESSION   DAOQFT010000363 DAOQFT010000000
VERSION     DAOQFT010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25206_length_2587_cov_5.785150"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLR93_09390"
     CDS             <1..1029
                     /locus_tag="PLR93_09390"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH27221.1"
                     /translation="AQEKYEQTLEIISTSSILGRARVLGKCAKVLGNLKEYEKSKLLF
                     AQAEKELGIPPGEENIEWWQTRLKVQFDQIWMIHDSYNIAGMQEVFEVVRPVVERLGE
                     TDRLAEYYFLLPTLYFNSNGYQADEKIIEYSTLALELSLKGTDLELQSRTQFGYGFVQ
                     LLIGNIGLAIKHLEEGLRLSTQIGYLDQQTYCITYLAVAHRRAGNLEECRQYSEQMLV
                     ISEREGQGSFTASAFANLGWLAWKQNDFAKAKTLSKKAAALWHPRYAFHWHGLWTLID
                     ISLRSHKVDEAVENVRRLRAPSQHVLSKELDDLMVDIIGQADKGNISNSESLLKQAVA
                     WVKEHHYL"
     gene            1103..1504
                     /locus_tag="PLR93_09395"
     CDS             1103..1504
                     /locus_tag="PLR93_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNH27222.1"
                     /translation="MSVETTKEVLARYLNSGHSDQSMLAEDVSFTIMATGKEHRGREG
                     VSAMLNYFYRIAFEADAKTRTLVFGETSAIWEGVIVGKHIGEYASVPATGKNVRVPIC
                     VVYDVEDKRVKRARVYFEVPAFYQQVGVKMG"
     gene            1727..2089
                     /locus_tag="PLR93_09400"
     CDS             1727..2089
                     /locus_tag="PLR93_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27223.1"
                     /translation="MKALRNTLIALAVYMTVVGALFLFAPSVAEKAFSTSLPDRALTP
                     LYGELMIVIALMAYLVSTDVSAYKKLVIAFIVSEIGHVLIFGYQLMTGIATFAQVGPP
                     MVIAIIFTVLLFVFKGKA"
     gene            2101..2319
                     /locus_tag="PLR93_09405"
     CDS             2101..2319
                     /locus_tag="PLR93_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27224.1"
                     /translation="MESDEAGARTKSLLFTLRLWSADENTHAPQWRVRLQNVHTGEVH
                     YCGDEKSLNTLLDDILSEVLKEKRKEER"
     gene            2328..>2587
                     /locus_tag="PLR93_09410"
     CDS             2328..>2587
                     /locus_tag="PLR93_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27225.1"
                     /translation="MLLLLLLILSLILSACGSSPAPTHAPTSGGFDIKPFLGVYEGTW
                     TNKTTGASGPAVIIVEAKEADKTVTLTLDFDGNYLGLNDPPA"
BASE COUNT          663 a          603 c          660 g          661 t
ORIGIN      
        1 gcacaggaaa aatatgaaca gacacttgag atcataagca cttctagtat tctcggacgg
       61 gctcgcgtgt tgggaaagtg tgcgaaggta ttgggtaatt tgaaggagta tgaaaagtcc
      121 aagttgctat tcgctcaggc tgaaaaagaa cttggcatcc ctcccggcga ggaaaatatt
      181 gaatggtggc agacccggct caaggtccag tttgaccaga tatggatgat ccatgattca
      241 tataacatcg cagggatgca ggaagtcttt gaggttgtgc gtcctgttgt ggaacgtctt
      301 ggtgagacgg acaggcttgc cgaatattat ttcctgttgc ctactttgta tttcaatagc
      361 aatggctatc aggcagacga aaagatcatt gaatattcca cgcttgcttt agaactaagc
      421 ctcaaaggaa ccgacctcga attgcagtca cgtactcaat tcggttatgg gtttgtacaa
      481 ctgctgatag gaaatattgg acttgcaatt aaacacctgg aggaaggtct gcgcctctcg
      541 acgcagatcg gatacttgga tcagcagacc tattgcatta cgtatttggc ggttgcgcat
      601 cggcgagctg gaaatttgga agaatgccgc cagtactctg agcaaatgct ggtaatcagc
      661 gaacgggaag ggcagggcag ttttaccgct tccgcgtttg ccaatttagg ctggctggca
      721 tggaaacaaa atgactttgc caaagcaaaa acactttcca aaaaagcggc tgcactctgg
      781 catccgcgtt atgctttcca ctggcatggc ttgtggacgt tgatcgatat cagcctgcgt
      841 tctcacaaag tggatgaggc ggttgagaat gtgcgtcggt tgagggcacc gagtcagcat
      901 gtgttatcga aagagttgga tgatctaatg gttgatatta tcggtcaggc ggataagggt
      961 aatatttcca acagtgaatc tttgctaaag caagccgtgg cttgggtaaa ggaacatcat
     1021 tacctgtaac gttacaatga aacggttttg aaacggcttc tccgtacact ggggacaact
     1081 caacaactta aggagaagca ccatgtctgt cgaaactaca aaagaagtac tggcacgcta
     1141 cctcaactct ggtcacagtg accagagcat gctggcagag gatgtcagct ttaccatcat
     1201 ggcaaccggt aaggaacacc gtggtcgaga aggtgtttcg gcgatgctga attatttcta
     1261 tcgcatcgct tttgaagcag atgccaagac tcgcaccctg gtcttcggcg aaacgagcgc
     1321 catttgggaa ggtgtcattg tggggaagca cattggcgaa tatgcaagcg tacctgccac
     1381 aggaaagaat gtccgagtgc cgatctgtgt ggtgtatgac gtggaggata agagagtcaa
     1441 gcgggctcgg gtctattttg aggtccctgc tttttatcag caggtcggcg tcaaaatggg
     1501 ataacctccc ctcttaatgc aaagtcgcta agccgccatg tttcaaattc ctttctttgc
     1561 gaccctgcgc ctttgcgtta aaaatataca aggtgaaaaa agctatgatc cccgccttct
     1621 aggcggggat tttctttccc gagaaacgct ccatgaaacg cttttgaaac gccccatcca
     1681 taaaatgagc acagttcgat atttcaactc atttacggag gctcttatga aagcgttacg
     1741 aaataccctt attgcattgg ctgtctacat gacggtcgtc ggagctttgt tcctctttgc
     1801 tcccagcgtg gcggaaaagg cattcagtac atccctgcca gaccgcgccc tcaccccgct
     1861 ctacggcgaa ttgatgatcg tcatcgccct gatggcatat ctagtttcca ccgatgtctc
     1921 cgcctacaag aaattggtca ttgcattcat cgtttcggaa attgggcatg tgctcatctt
     1981 cggctaccag ctaatgaccg gcattgccac cttcgcccag gtcggtccgc ccatggtcat
     2041 tgccatcatc ttcaccgtcc tgctctttgt cttcaagggc aaagcctaaa aggttgtgat
     2101 atggaaagcg atgaggctgg cgctcgcaca aaatcgctcc tgtttacctt gcgtctttgg
     2161 agtgccgatg agaacacgca cgcgccgcaa tggcgcgtgc gtctccaaaa tgtacacacg
     2221 ggcgaggttc attattgtgg ggatgagaag tcgctgaaca cattgctcga tgatattttg
     2281 agcgaagttc tcaaggaaaa aagaaaagag gaacggtgat tagaaaaatg ttattacttc
     2341 ttttgcttat tttgtctttg atattgtcag cctgcggttc ttcccccgct ccaactcacg
     2401 ctccaacttc aggcggattc gacatcaagc cgtttctcgg cgtctacgaa ggcacttgga
     2461 ccaacaagac cactggcgcg agcggaccgg cagtcatcat agttgaagcg aaagaagccg
     2521 ataagacagt gaccctcacg cttgattttg acggcaacta tcttgggttg aacgacccgc
     2581 cagcctt
//
LOCUS       DAOQFT010000364         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25245_length_2585_cov_4.947431, whole genome shotgun sequence.
ACCESSION   DAOQFT010000364 DAOQFT010000000
VERSION     DAOQFT010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25245_length_2585_cov_4.947431"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..88
                     /locus_tag="PLR93_09415"
     CDS             5..88
                     /locus_tag="PLR93_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27226.1"
                     /translation="MCDYIAGMTDRYAIEEHQKMFNPNEKP"
     gene            178..642
                     /gene="greA"
                     /locus_tag="PLR93_09420"
     CDS             178..642
                     /gene="greA"
                     /locus_tag="PLR93_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNH27227.1"
                     /translation="MTQPNYLTPEGEAKLKAELEELKGPRREELAQRLRSAIQMGDLS
                     ENADYHKAKEDQGFLEGRIQEIEAILRNSVLIEKTASSDVVSIGSHVTIQEEDFDPET
                     YHIVGPTEADPRKGKISHESPIGVALMNKKVGQIAEAQAPGGKIKFKILKIE"
     gene            complement(687..1685)
                     /locus_tag="PLR93_09425"
     CDS             complement(687..1685)
                     /locus_tag="PLR93_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27228.1"
                     /translation="MDFVVAFLKKLPVVPIVLFVVGLALGLLIGWGIAPVEFKDATPY
                     YLRPDLREDYMRMTIDSFSINKSAALAADRWLGLGSTAQEAYLAIQNNPGYLPSALVQ
                     EFGQAVTNEINARGGVPADPSTGMSPTMRTALIGIGVILLLGVVAAGGMYLFRLFGKR
                     GSGEVTAVMQAAEISRTAAKTNFEDMGLAPPITQTMTTYVLGDDLYDESFSIDTAAGE
                     FLGEYGVGVSEIIGVGEPKKVTALEIWLFDKNDIKTATKVLMSRHAFADPGIRSRLEP
                     KGELVLVEPQAQVLLETATLQLLATIVDIEYGKGPLPDSSYFERVTLELAIWPRQG"
     gene            complement(1838..>2585)
                     /locus_tag="PLR93_09430"
     CDS             complement(1838..>2585)
                     /locus_tag="PLR93_09430"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNH27229.1"
                     /translation="RLIPEQHFTQPPPRFSEASLVQALEENGIGRPSTYAPTISTIQQ
                     RGYALREEKRLVPTDMGIQVNDLMAQYFPEVVDLNFTAHMEEDLDKIADGQMEWVDAI
                     REFYGPFAEDVKKAQAEMPVAKSEPEPIGRKCPDDGGELVIRYGRFGKFISCANFPTC
                     RYTEPWLEKIGVSCPKDGGDMVERRTRKGRTFYGCANYPNCDFTTWKKPLAKPCPKCN
                     GLLVIANKREAQCTNCSESFLLEEIVPENA"
BASE COUNT          726 a          681 c          576 g          602 t
ORIGIN      
        1 aaccatttgc gattacatcg ccggcatgac agatcgctac gccatcgaag aacatcaaaa
       61 aatgttcaac cccaacgaga aaccttaatt tctttcgttt ctaaaggggg catagagata
      121 gagttcgtat caagcccctt atcgtcttat ccaacgcaca tcaggaaagg agaaaccatg
      181 acacaaccca attacctgac ccccgagggt gaagccaaac taaaggctga gctcgaagaa
      241 ctgaagggac ctcgccgcga ggaattggca cagcgcctgc gttctgccat ccaaatggga
      301 gacctctccg aaaatgccga ttaccataag gcaaaggaag atcaaggctt ccttgaaggt
      361 cgcattcaag agatcgaagc cattttgcgc aactcagtac taattgaaaa gacagcctcc
      421 tcagatgttg tctccattgg ctctcatgtg accattcaag aagaagactt cgacccagag
      481 acgtatcata tcgtcggtcc gaccgaagca gacccacgca aaggcaaaat ctctcacgaa
      541 tccccaattg gggttgcgct gatgaacaaa aaggtcgggc aaatcgctga agcacaagcg
      601 cccggcggca aaatcaagtt caaaattcta aaaattgaat aaacaaaaaa acgctccacg
      661 tatgtggagc gtttttttgt ttatatttat ccttggcggg gccaaattgc taattcaagt
      721 gtcacacgct caaagtagct gctatcaggc aaaggtccct tgccatattc aatatccaca
      781 atggttgcga ggagttgcag ggttgccgtt tcgagcaaca cctgagcttg aggttccaca
      841 agaaccaact cacccttagg ttcgaggcgc gagcgaattc ctgggtctgc aaaagcatgg
      901 cgagacatca gaactttggt cgctgttttg atgtcgttct tatcaaaaag ccaaatttct
      961 aacgcagtaa ccttcttcgg ctcacccacg ccaataattt cagaaacgcc cacgccgtat
     1021 tcgccaagga actcacctgc tgctgtgtca atgctgaaag attcatcgta aaggtcatca
     1081 cccaagacat aagttgtcat ggtctgcgta atgggtggag ccagacccat atcttcaaaa
     1141 tttgtcttgg cggcagttcg gctaatttca gcggcttgca tcactgcggt cacttcgcca
     1201 gagcctcgtt tgccaaacaa gcggaagaga tacatgcccc cggcggcaac gacgcccagt
     1261 aacaaaatca caccaatgcc gatcaatgca gtgcgcatgg taggagacat acccgtagag
     1321 gggtcagcgg gtacgccacc gcgtgcattg atctcattcg tcactgcctg accaaactct
     1381 tgcaccaaag cagaaggtaa ataaccgggg ttattttgaa tcgccaaata ggcttcttgc
     1441 gctgtgctgc ccaaccccaa ccaacgatcg gctgccaatg ctgcgctttt gttaatgcta
     1501 aaagagtcaa tcgtcatgcg catgtaatct tcgcgcaaat cagggcgcaa ataatatggg
     1561 gttgcatcct taaattctac gggcgcgatt ccccaaccaa tcaacagccc aagtgccaac
     1621 cctacaacaa aaaggacaat tggcacaaca ggcaacttct ttaaaaatgc aacaacaaaa
     1681 tccataattg gctccttcgc acagatgctt tcgctcgatt atacaacgta aattaggggc
     1741 actgtatgac agaatgattg cccacatgga taaaaaaacg catcagactc agccatactt
     1801 gccactacga ccaagcctga tgcgaacgct gaaagtttta tgcgttctct ggcacgattt
     1861 cttccaacaa gaaactttca gaacaattcg tgcactgcgc ctcgcgtttg tttgctatca
     1921 ccaacaaccc attacatttc gggcaaggtt tggctaaggg cttcttccat gttgtgaaat
     1981 cacaattcgg gtaattcgca caaccataga aggtgcgtcc cttgcgagtg cggcgttcca
     2041 ccatatcgcc gccatctttc gggcaggaaa caccgatctt ctccaaccac ggttctgtat
     2101 agcgacaggt tgggaagttt gcacaggaaa tgaacttgcc gaagcgccca taacggataa
     2161 ctagctcacc gccatcatcc gggcattttc gtccaatcgg ctccggctca gacttggcaa
     2221 cgggcatttc tgcctgtgcc tttttcacat cctcagcaaa cggaccgtag aactcacgaa
     2281 tggcatccac ccattccatt tgtccatccg caattttatc gaggtcttct tccatatgag
     2341 ccgtaaagtt caaatcgact acttcaggga agtattgagc catcaagtca ttgacctgaa
     2401 tccccatatc ggttggcacc agtcgttttt cttcgcgcaa ggcatatccg cgctgctgaa
     2461 tggttgaaat agtcggcgca taggtggacg gacgaccaat cccattctct tccagggctt
     2521 gtacgagtga ggcttctgaa aagcgtggag gaggttgagt aaagtgctgt tcaggaatta
     2581 aacgg
//
LOCUS       DAOQFT010000365         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25316_length_2581_cov_8.283848, whole genome shotgun sequence.
ACCESSION   DAOQFT010000365 DAOQFT010000000
VERSION     DAOQFT010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25316_length_2581_cov_8.283848"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLR93_09435"
     CDS             complement(<1..633)
                     /locus_tag="PLR93_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNH27230.1"
                     /translation="MKGFFSSALARGLFWQLIGTLVGAGLVTGIRALMGLNALDTFFF
                     TEPAWVLGGFFGALFFLAGSGVVSDWFKWARGIDTPDHHEDHHTGAAKYINVSLDHKV
                     IGVQYTITALLLIAIGGLFALIFRTELAASQLQFLTTDMKLFSQNGPQLYNTLMSLHG
                     MIMIVSILLGTAGFINYVVPLLLGAQDMAFPRLNAFSFWIAVPGAVLLLMS"
     gene            complement(655..1665)
                     /gene="coxB"
                     /locus_tag="PLR93_09440"
     CDS             complement(655..1665)
                     /gene="coxB"
                     /locus_tag="PLR93_09440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNH27231.1"
                     /translation="MAHFVIVGILVIVMTILTYFGLDAAGLATQMHPVAASAQAAPID
                     QLWHWEIIVISFLLSLIVVPIVYSLVVFRQKKGELKDGEHIEGNTALEIGWTVVPLLI
                     VVVFAYLGAYSLGEVRRVDPNAMVVNVKAQQFSWTFEYPEYGFITTQLYLPIDRQVVL
                     KMESADVIHSFWVPEFRIKQDVVPGRITEYRVTPSLEGSYKVRCAELCGTSHAYMEAP
                     VVVVNSDAYDAWIVEQIKIAEESKTPEGQGKLLATKNGCVGCHSADGTAMTGPTWFNL
                     YGSEVKLADGSTVTADDAFITESILQPTAKVVNGFPPVMPPYTLTDEEISYIIAYIKT
                     LK"
     gene            complement(1934..2398)
                     /gene="bcp"
                     /locus_tag="PLR93_09445"
     CDS             complement(1934..2398)
                     /gene="bcp"
                     /locus_tag="PLR93_09445"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNH27232.1"
                     /translation="MPVTSGIPAPTFELLDDTNTLRKLSDYRGKNVVLYFYPKDDTPG
                     CTKEACNFRDDYSAYERAGVVILGISPDSVESHVKFKKKFQLQFPLLADDGHKVCDLY
                     QVWGPKKFMGKAYEGVLRTTFLIDADGNIKKVYEDVRPAEHSQELLAELGLK"
BASE COUNT          778 a          607 c          572 g          624 t
ORIGIN      
        1 gctcatgagc agaagcacag ccccagggac agcaatccag aatgagaagg cattgaggcg
       61 cgggaatgcc atatcctgcg caccgagcaa caggggaacg acatagttga tgaagccagc
      121 tgtgcccaac aagatcgaga cgatcatgat catgccatgc aaggacatca atgtgttgta
      181 caactgaggt ccattctggc tgaacaattt catatcagta gtgaggaatt ggagttgaga
      241 tgcagccaat tctgtacgaa agatcaaagc aaataaaccg ccaatggcga tcaacaacaa
      301 cgctgtgatg gtgtactgaa caccaatgac cttgtggtca agtgaaacgt tgatgtactt
      361 tgccgcgccg gtgtggtggt cctcatggtg atctggcgta tcaatgccac gcgcccactt
      421 gaaccaatcg ctcacaaccc cagagccagc gaggaagaaa agtgcaccga aaaagccgcc
      481 caacacccag gctggttcgg taaagaagaa cgtatcaagc gcattcaacc ccatcaatgc
      541 gcgaatgcca gtgacaagac cagcaccaac gagcgtgccg atcaattgcc aaaacaaacc
      601 gcgagcaagg gcactagaaa agaatccctt catagtattt aatccttatc aaatttattt
      661 caaggtcttg atgtatgcga tgatataaga gatctcttca tcggtcaaag tgtaaggcgg
      721 cataacgggc gggaagccat taacaacctt agctgttggc tgaagaatag actctgtgat
      781 gaaggcatcg tcggcagtca cagtggagcc gtcagcaagc ttaacctctg agccatacag
      841 gttgaaccaa gtcggtcctg tcatagctgt gccatccgca gagtgacaac caacacagcc
      901 attcttggtt gcaaggagtt taccctgccc ttcgggggtc ttggactctt cggcaatctt
      961 gatttgttca acaatccagg catcatacgc atctgaattg acaacgacaa caggagcttc
     1021 catataagca tgggatgtac cgcaaagctc ggcacagcgg actttgtagc tgccctcaag
     1081 actcggagta acacgatact cggtgatgcg tccaggtaca acgtcttgtt tgatgcggaa
     1141 ctcaggaacc cagaatgagt ggatcacatc cgcagactcc atcttaagca caacctggcg
     1201 gtcaatgggc agatagagtt gagttgtaat aaacccatat tcggggtatt caaaagtcca
     1261 tgaaaattgt tgggctttga cgtttacaac catggcgttt gggtctacac ggcggacttc
     1321 accaagtgaa taagcgccta aataagcaaa aacaaccacg atgagtaatg ggacaaccgt
     1381 ccaaccgatt tcaagggcag tattcccctc tatatgttcc ccatccttaa gttcgccttt
     1441 cttttggcga aacacaacca aactatatac aatcggtaca acgatcagcg aaagtaggaa
     1501 ggaaattacg atgatttccc aatgccataa ctgatcaatg ggagcagctt gtgcgctggc
     1561 tgccacagga tgcatttggg ttgcaagccc agcagcatca agaccgaaat acgtgagaat
     1621 ggtcatcaca atgaccaaga ttcccacaat tacaaaatgg gccatatact ctctctcctg
     1681 aaaagattga acggggcatg agctccgatg attaactaaa attggacaaa tacagttaga
     1741 attgtctcaa ttttcacaat atcagattat aacaaaatcc ctttttattg tcacgctatc
     1801 ttggtcaaat gcacagctat tcttaacttg cacaaacctc gattttcaca aatcatgccg
     1861 acggtgtttg gataccgtcg gcaatctaaa agctatatca agctataaca cgatacgttg
     1921 tcgtagagat gcgttatttc aagcctaact ctgctaaaag ttcctggctg tgttcagctg
     1981 ggcgcacatc ttcatatact ttcttgatgt ttccgtcagc gtcgatcaaa aacgttgtac
     2041 gaagaacacc ttcatatgct ttacccataa actttttagg tccccacacc tgataaaggt
     2101 cgcaaacttt gtgtccatca tcagcaagca agggaaattg aagctggaat ttctttttaa
     2161 acttcacatg agattcaacg gagtctgggc taatgcctag aatcaccacc cctgcccttt
     2221 cgtaggcaga gtaatcatcg cgaaagttgc acgcttcttt tgtgcaaccg ggcgtatcat
     2281 ctttcggata aaaatataag acgacattct ttccacgata atcggaaagt ttgcgtaagg
     2341 tattggtatc gtcaagcaac tcaaatgtag gggcaggaat gccagaagta acgggcataa
     2401 aaaagtactc tctctccaaa gaagtatgcg cgttattata cctttattca ttttaagggt
     2461 aggaaaactt tcacaattat ttggtgggca gcactccaat tttcggtaga aaaccggctt
     2521 taagcatgga tcaatctcct tataaaaaac ctcagaaaaa atggcgattc gggcattccc
     2581 t
//
LOCUS       DAOQFT010000366         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25550_length_2567_cov_9.232882, whole genome shotgun sequence.
ACCESSION   DAOQFT010000366 DAOQFT010000000
VERSION     DAOQFT010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25550_length_2567_cov_9.232882"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..2149
                     /locus_tag="PLR93_09450"
     CDS             80..2149
                     /locus_tag="PLR93_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH27233.1"
                     /translation="MTNNIERHAKLLKASARAAKNITTILDPYELFQRTVDIICDEFG
                     FYYAGVFLVDDASQYAVLKAGRGDAGKAMLREEHKLAIGGNSMIGASIANKKGRIALD
                     VGEEAVFFENPHLPKTRSEMALPLIIADEVIGALTVQSTEEAAFHEEDIDALQTMADQ
                     LAVAIQNSKLHRQEERRSRLLKAANRVGKEVASLLDLDQLLPQTVNIICEAYGLYYAG
                     VFLLDEKGEYAVLRAGYGKAGKAMLSEGHKLRVGTDSMIGACVAMGEARIALDVGEER
                     VHFKNPHLPHTRSEMALPLIYGGKTLGAVTIQSSEERAFSDDDITTLLTMAEHLAVAI
                     NNAKLIDELKEAHNEILRNKVFEALTSASTEAIHWIGNKTLPISLTVSRLREEIADGK
                     VDVESLKEDLDMISESAAQIIQVKEQLIGAVREMKPRPVLFADVLQTAAMQRGIAQNA
                     LKVSIDPEAAYVIADSTQLTRAVGNILQNAVEAGAKSVKVRVHPTEERGVLEINIEDN
                     GSGMNDETQRKVWSPFYTTRGVSHHGLGLPAAMHVVSQSQGRIALVSEEGKGTTVALF
                     MPQGRVTSSEVQPGGVKNILLIDDNDDWSNLLTEMFKGSKVKLTQTTDLKKFPEADLI
                     LVDENIASAPLTDVLAALSKAGLASKTVVLTSAINPERVTQYLREGLKDVTVKPYSVN
                     ELNELLK"
     gene            2203..>2567
                     /locus_tag="PLR93_09455"
     CDS             2203..>2567
                     /locus_tag="PLR93_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27234.1"
                     /translation="MRPRWRKVFHDLWDNFMRTALVVLSIAVGVFSIGVITGAYEIIS
                     NDMGESYAANNPANIELRMTNFNDDVLSSIRNFKGVQDAEGRRVINYRVHPEGSTQWS
                     TIDLIAVEDFSKNNVNLLVP"
BASE COUNT          663 a          579 c          663 g          662 t
ORIGIN      
        1 aaaggacgtg agcaaggttt ggtggagttg atccaaaaaa tcttgagtgg aaattaattc
       61 actttcaaca gaggaactta tgacaaataa tattgaacga catgcaaaat tattgaaagc
      121 ctccgcgcgt gccgcaaaga acattaccac tatccttgac ccctacgagc ttttccagcg
      181 tacagtggat atcatttgcg atgagtttgg cttttactat gccggtgtct ttttggtaga
      241 tgatgccagt cagtatgctg tgctcaaagc tgggcgtggc gatgctggta aagccatgct
      301 gcgcgaagaa cataaactgg caattggcgg caattccatg atcggtgcca gcatcgcgaa
      361 taagaaaggt cgcatcgcgc tggatgtggg cgaagaagcg gtattttttg aaaacccgca
      421 tttgcccaag acccgttctg agatggcact tccgctcatc attgccgatg aagtgatcgg
      481 tgcgcttacg gttcagtcga ccgaagaggc tgctttccac gaagaagata ttgacgcact
      541 gcagaccatg gcagatcagc ttgcggttgc tattcaaaac tccaagttgc accgccaaga
      601 agaacgccgc tctcgcttgc tcaaggctgc gaatcgagtt ggaaaagaag ttgcatctct
      661 tctcgacctc gaccagctct taccgcagac cgtgaatatt atttgtgaag cctatggctt
      721 gtactatgca ggcgtgttct tgctcgatga aaaaggtgaa tatgctgtct tacgtgcagg
      781 ttatggcaag gctggcaaag ccatgctttc tgaaggtcat aagttgcgcg tcggtaccga
      841 ctcgatgatc ggtgcttgtg tcgcaatggg cgaggcacgt attgcgttgg atgtaggcga
      901 agaacgggtg catttcaaga atccgcattt gccgcacact cgctctgaaa tggcgcttcc
      961 gctgatttat ggtggcaaaa ctctcggggc ggtcaccatt caatcttctg aagaacgtgc
     1021 tttcagtgat gatgacatta ctactctgtt gacgatggct gaacacttgg ctgtcgcaat
     1081 caataatgcc aaactgatcg acgaacttaa ggaagcccat aacgagatcc ttcgcaacaa
     1141 agtctttgaa gcgctcactt ccgcttctac cgaagccatt cactggatcg gcaataagac
     1201 gcttcccatc tcgctgacag tttcccgctt gcgcgaagaa attgccgatg gcaaagtgga
     1261 tgtcgaatcg ctcaaagaag atctcgacat gatctctgag agtgctgcac agatcattca
     1321 ggtgaaggaa caactgatcg gtgccgtccg tgagatgaaa ccgcgcccgg ttctctttgc
     1381 tgatgtgctt caaaccgcag ctatgcaacg cggcattgcc cagaacgctt tgaaggtatc
     1441 cattgaccct gaggctgctt atgttattgc agactccact cagcttaccc gtgcagttgg
     1501 caacatttta caaaacgctg ttgaagcggg tgccaagtcc gtcaaagtgc gggtgcatcc
     1561 taccgaagag cgcggcgtgc ttgaaattaa catcgaagat aatggttctg gcatgaacga
     1621 tgaaactcag cgcaaagttt ggtcgccttt ctacacgaca cgtggtgttt cacatcatgg
     1681 tttggggttg cctgcggcaa tgcatgtcgt ttcgcaatca cagggtcgca ttgcactcgt
     1741 cagcgaagaa ggtaagggaa ctaccgtcgc gttgtttatg ccccagggac gtgtgaccag
     1801 ttccgaggtc cagccgggag gcgtaaagaa catcctgttg atcgatgata acgacgattg
     1861 gtcaaatctg ctcacagaga tgttcaaagg ttcgaaagtc aagttgaccc aaacgacaga
     1921 cttgaagaaa ttccctgaag ccgatctgat tttggtggat gagaacatcg cctcggctcc
     1981 gctgaccgat gtgttggctg ctctttccaa ggctgggtta gcatcgaaga ctgtggtcct
     2041 cacctctgcc atcaaccctg agcgtgtgac ccaatatctg cgcgaaggtt tgaaagatgt
     2101 aacggtcaag ccgtattctg taaacgaatt gaatgaactg ttgaagtaaa ggaaagaaag
     2161 ttgacaggtc ttcaagacct gtcaactttg taaaagagaa acatgagacc acgttggcgt
     2221 aaagtatttc acgatttatg ggataacttc atgcgtactg cattggtggt tttatccatt
     2281 gcagtgggtg tgttttccat tggggtaatc actggtgcgt atgagattat ctcaaacgat
     2341 atgggagaat cctacgcggc aaataaccct gcgaatattg aacttcgcat gacgaatttt
     2401 aatgacgatg tactgtcgtc tattcgtaat ttcaagggtg ttcaagatgc tgaaggcagg
     2461 cgtgtgatta actatcgcgt ccatcctgag ggcagtactc aatggtcgac cattgacttg
     2521 attgcggtag aagacttcag taagaataat gttaatttat tggtgcc
//
LOCUS       DAOQFT010000367         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25599_length_2564_cov_4.518533, whole genome shotgun sequence.
ACCESSION   DAOQFT010000367 DAOQFT010000000
VERSION     DAOQFT010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25599_length_2564_cov_4.518533"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLR93_09460"
     CDS             <1..370
                     /locus_tag="PLR93_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNH27235.1"
                     /translation="DIAMYDLPHYRSLQSLIAHGLLITIPIFMTVVEGYRPTFASFKR
                     IFIWTNIYMVIIFFLNRVIGSNYLFIAQKPPSPTLMDMLSPWPWYIPQLEVVAFIIFF
                     ILYIPFLIKDQRAKQSVRQA"
     gene            385..1308
                     /locus_tag="PLR93_09465"
     CDS             385..1308
                     /locus_tag="PLR93_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNH27236.1"
                     /translation="MKTKYFFFLISALIFVSMACRKTANTGVGIQNEYTLNYQNIERS
                     YILYTPKGLDTSKPMPLVFVLHGGGGNAENIIRSSGFNEVADQNGALIVYPNGTGRLN
                     DAILTWNGGTCCGFAQENNIDDVGFIREILKDVESIENVDPKRIYATGMSNGGIMSYR
                     LACEASDIFAAVGSVSGTLNFSPCTPSENVSVIHFHGTDDQHVPYNGGVGSQSLVGVD
                     FASVQDSVNFWVEFNGCNSQPQTNSFEDVQHQAWEGCVGFSSVELYTIIGGKHAWPGS
                     QGPGWVGGDEPDQTISATQLLWEFFASHPKP"
     gene            1405..2532
                     /gene="hppD"
                     /locus_tag="PLR93_09470"
     CDS             1405..2532
                     /gene="hppD"
                     /locus_tag="PLR93_09470"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNH27237.1"
                     /translation="MVAKAKNESRPMTEEADFLQIKSFDHVHFYVGNAKHAMYYWWKA
                     FGFKPVAYSGLETGNRDFASYVLESGQARFVVSAPYSPSSPLASHHMVHGDGVKVIAL
                     EVDDVEKAWRETTSRGGKSAWAPREEKDDHGIYRSSAIYTYGETIHAFIDRDDYKGTF
                     APTYRPLKHEAESTGLAAIDHIVGNVQLGKMNHWVNFYHQVMGFRQLMHFDDKDISTE
                     YSALMSKVMQNGNGRVKFPINEPADGKRKSQIEEYLDYYMSPGAQHIAIITGDILSTV
                     ESLRKNGVEFLRVPDTYYEMLPDRVGKIKEDFKTIHELGILVDKDDEGYLLQIFTRPI
                     QDRPTMFIEIIQRHGAQGFGKGNFKALFESLELEQERRGNL"
BASE COUNT          678 a          622 c          569 g          695 t
ORIGIN      
        1 ggatattgct atgtacgacc ttccgcacta tcgcagttta caatcgctaa tcgcccatgg
       61 attgctaatc accattccaa tttttatgac cgtggttgaa gggtatcgcc caacatttgc
      121 gtctttcaag cgcatcttta tctggacgaa tatttatatg gtcattattt tctttctcaa
      181 ccgcgtcatt ggcagcaatt acctgtttat cgctcaaaag ccaccttccc ccactctcat
      241 ggacatgctc tctccctggc cctggtatat tccacaactt gaggtggtgg cgttcattat
      301 tttcttcatt ctgtacatcc cgttcctcat taaagatcaa agagcaaagc agtcagttcg
      361 tcaggcgtaa tactagtttt gcccatgaaa accaagtatt tcttttttct aatctctgca
      421 ctgatatttg ttagcatggc atgtagaaaa acggcaaata caggtgtagg tattcagaat
      481 gaatacactt taaactacca aaacatcgag cggagttaca tcctttatac tcctaaaggg
      541 ctcgacacat ctaaacctat gccgctggta tttgtattac acggcggagg cgggaatgca
      601 gaaaatatta tccgctccag tggttttaat gaggttgctg accagaacgg agcattgatc
      661 gtttacccaa atgggacggg tcgactcaat gatgcaatcc ttacctggaa cggcggcact
      721 tgttgcggct ttgctcagga aaacaacatt gatgatgttg gtttcattcg tgaaatcctg
      781 aaggatgtgg agtccatcga aaatgttgac ccgaaacgaa tctacgccac agggatgtcg
      841 aacgggggca tcatgtcata tcggcttgca tgtgaagctt cagatatttt tgcagccgtt
      901 gggtctgtct ctggcacgct taatttttct ccttgcacgc cgagcgaaaa tgtttccgtc
      961 attcattttc acggcacaga tgaccagcac gtcccttata acggcggtgt agggtctcaa
     1021 tcgctcgtag gggtagattt cgcctcggtt caagactctg tcaatttttg ggttgaattt
     1081 aatggctgta actcacagcc acaaaccaat tcttttgagg atgttcagca tcaggcgtgg
     1141 gaaggatgcg taggtttttc ctccgtggag ttatacacta tcattggagg aaaacacgcc
     1201 tggcccggca gccaaggtcc cggttgggtc ggcggcgatg aaccggatca aaccatttct
     1261 gccactcaat tgttgtggga atttttcgca tctcacccaa aaccttaatt tattttttaa
     1321 ttgcatccat aggtctttta tcgtagaatc ctaaagacgt tattcatccc atatccatgg
     1381 gaaaaccaac ctaaaggaga gaaaatggta gcgaaagcca agaatgaatc acgccccatg
     1441 accgaggaag cagacttcct ccaaataaaa tcgttcgacc acgtccactt ttatgtgggc
     1501 aacgccaaac atgccatgta ttattggtgg aaggcatttg gcttcaaacc tgttgcgtat
     1561 tcagggttag aaactggcaa ccgcgacttt gcctcgtatg tacttgaatc ggggcaagcg
     1621 cggtttgttg tttctgcacc ttattcccca tccagcccac tcgcatcaca ccacatggtt
     1681 catggcgatg gcgttaaagt cattgcgctg gaagtggatg atgttgagaa agcctggcgt
     1741 gaaaccacca gccgcggagg aaaatctgcc tgggcaccgc gtgaagaaaa ggatgatcat
     1801 ggcatttacc gcagttctgc catttacacc tacggcgaaa ccattcacgc ctttattgac
     1861 cgtgatgact acaaaggtac cttcgcccca acctatcgtc cacttaaaca tgaagcggag
     1921 tctaccgggc ttgccgccat tgaccacatt gttggcaacg tgcagctcgg caaaatgaat
     1981 cactgggtaa atttttatca tcaggtaatg ggcttccgtc agctcatgca tttcgacgat
     2041 aaagacatct ccaccgaata ttccgccctc atgtccaagg tcatgcaaaa cggaaatgga
     2101 cgcgtcaagt tccccatcaa tgaacctgca gatggaaaac gaaagagtca gattgaagaa
     2161 tatctcgact attacatgtc tcccggtgcg caacatattg ccataatcac aggcgatatt
     2221 ctcagcactg ttgaaagtct ccgtaagaat ggtgtcgaat ttttacgcgt gccagatact
     2281 tactatgaaa tgctgccaga ccgtgttggc aagatcaaag aagatttcaa aaccatccac
     2341 gaactcggca ttcttgtgga taaagatgat gaaggctatc tgctgcaaat cttcacccgt
     2401 cctattcaag accgccctac catgttcatt gagatcattc aacgccatgg cgcgcagggt
     2461 ttcggcaaag gcaacttcaa agccttattc gagtctcttg agttggagca agaacgccgc
     2521 ggcaatcttt agcgcatgag cttctgcgga taaaaatcat cgcc
//
LOCUS       DAOQFT010000368         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25714_length_2556_cov_7.265894, whole genome shotgun sequence.
ACCESSION   DAOQFT010000368 DAOQFT010000000
VERSION     DAOQFT010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25714_length_2556_cov_7.265894"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLR93_09475"
     CDS             <1..1164
                     /locus_tag="PLR93_09475"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNH27238.1"
                     /translation="IKINNRKLKKVSMKKNTLLFITLIVLLTLVLSACGGGQQTAATQ
                     EPAVNVINASDVVAEGRLEPAHATNLTFQARGVVEDVLVKVGDTVSEGDVLARLANAG
                     AAEAQVVVAQNAYDTLLRNESGDRAKLWEVYMNAQIVRAEAEKKWEDVDEDAVKNRIE
                     DINAEIEDLKDTLKDAQDEFDKYKDLDEDNSKRKAADDDLKTAQDNLNAKIRELEEET
                     RSRDEVKAAYEAALDAEAEAKYQYEISLDGPNADQLTLAKANLDAAKDALSNYVITAP
                     FSGVVADVNVKAGEQVGTDTRVVSIIDDSQWFVKTTDVTELEVVSLSIGQAVTIRPDA
                     LTDLEMTGTVTEISSAYTQQGGDILYTVRVRVDGSDPRLKWGMTVETVFRTGE"
     regulatory      1202..1288
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      1368..1462
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1563..>2556
                     /gene="ruvB"
                     /locus_tag="PLR93_09480"
     CDS             1563..>2556
                     /gene="ruvB"
                     /locus_tag="PLR93_09480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNH27239.1"
                     /translation="MAKTPSKALDPEAKPDDRVDNALRPQKLNDLIGQNQVKENLSIL
                     IAAARQRSEALDHVLFYGPPGLGKTTLAHVLGNEMGVNVKVTAGPAIERAGDLAAILT
                     NLRAGDILFIDEVHRLGRAVEEVLYPAMEDFALDIVIGKGPSARSIRLKLPRFTVVGA
                     TTRLALVTAPLRARFGAVYRLDYYDLDAMKQIVGRAAGLLKVQADESGIAEVARRARG
                     TPRIALRLLRRVRDFAQVRADGVITQNVAKEALDLLQVDPLGLDDVDRRVLKTIIEKY
                     NGGPVGLNTIAASISEEQDTIMDVVEPYLLQLGFLDRTPQGRVATKSAYEHLGYQ"
BASE COUNT          682 a          562 c          703 g          609 t
ORIGIN      
        1 ataaaaatca acaatcgaaa attgaagaag gtttccatga agaaaaatac actacttttt
       61 ataactttga tcgtgcttct tacgcttgtt ctctcagcgt gtggtggcgg tcaacagacg
      121 gctgctactc aagaaccggc tgtcaatgtg attaacgcca gcgatgttgt tgctgaagga
      181 cggcttgaac ctgctcatgc cacgaatttg actttccagg cacgcggcgt tgtggaagat
      241 gtgcttgtca aagttgggga tactgtcagc gagggagatg ttttggctcg cctcgcaaat
      301 gcgggtgcgg cagaggcgca ggttgtcgtc gcacaaaatg cttacgatac gttgctccgt
      361 aacgagagtg gtgaccgtgc caagttgtgg gaagtatata tgaatgccca aatcgttcgc
      421 gcagaagcgg aaaagaaatg ggaagatgta gatgaagatg ccgttaagaa ccgtattgaa
      481 gatatcaatg cagagatcga agacttaaag gatacgttga aagacgcaca agacgaattc
      541 gataagtaca aggacctcga cgaagataat agcaagcgta aggctgctga tgacgacctc
      601 aaaacagcac aagataacct caatgcgaag attcgtgagt tagaagaaga gacccgttca
      661 cgcgacgaag tgaaagcggc ttatgaggct gctcttgatg cagaagcaga agctaaatat
      721 caatatgaga ttagcttgga tggtcccaat gctgatcagc taacacttgc caaggcaaac
      781 cttgatgcgg cgaaagatgc cctttctaac tatgtcatca cagctccatt ctctggcgtt
      841 gtagcagatg tgaacgtcaa ggctggcgaa caggttggca cagatactcg tgtggtaagt
      901 atcattgatg atagccaatg gtttgtgaag accacggatg tgactgagtt ggaagttgta
      961 agtttgagca ttggtcaggc agttaccatt cgccctgatg ctctgaccga ccttgaaatg
     1021 actggtactg ttaccgagat tagcagtgct tacactcaac aaggcggcga tattctttac
     1081 accgtccgcg ttcgtgtgga tggttcagat ccacgcttga aatggggtat gacggttgaa
     1141 accgttttta gaacaggtga gtgattgaaa ttgcacgaaa agagtgcaat aataaaattg
     1201 ctccgagaaa gagcaaaacc gggaaaaccc ggcggcgcaa agccctactc taaagctgaa
     1261 ctgagccatg agtgtgaggg ttgccgataa aagcaaaacc gttaaaagac ggcggcgcaa
     1321 agtcagagcg tctacctctg gttactgtta ccggataaga ccgaccgact gccgaaaggc
     1381 aaacccgggg aaacccggcg acgcaaagtc atgggtctac aatcgtgatt tgatatacga
     1441 tcaagatcgc cagactgccg aagagcaaac cgtttgcagg gatgacattt taggtcatcc
     1501 ctgcttggtt ttaagctaat tgtttgcagg gcaagctacc tgtaaagggt ataatcgcgc
     1561 acatggcaaa aacaccttcc aaagcactcg accctgaagc caagcccgat gaccgcgtgg
     1621 ataacgcatt gcgcccacaa aagctcaatg acctcattgg acagaatcag gtaaaagaga
     1681 atttatccat tctcattgcg gcggcgcgtc aacgtagtga agcgttggat catgtcttgt
     1741 tttatggacc tcccggcttg ggaaagacca cacttgcgca cgtgcttggg aacgagatgg
     1801 gggttaatgt caaagtcacg gcaggacccg ccattgagcg tgctggtgat ttggctgcca
     1861 ttctcacgaa tttacgtgct ggcgatattc tctttattga tgaagtccat cgccttgggc
     1921 gggctgtgga agaagttctc tacccagcta tggaagattt tgctttggat attgtcatcg
     1981 gcaagggacc ttcggctcgt tccatccgcc tgaaactgcc tcggtttacg gtagtggggg
     2041 ctacgacccg gctggccctc gtcacggcgc cgttgcgcgc gagatttggc gcggtctatc
     2101 gtttggacta ttacgatttg gatgccatga agcagattgt tggacgggca gcagggttgt
     2161 taaaagttca ggcagatgag tcaggcatcg cggaggttgc gaggcgcgca cgcggtacgc
     2221 cgcgtattgc tttgcggttg ctgcgtcgtg tgcgagactt tgcccaagtc cgcgcggatg
     2281 gtgtcatcac ccaaaacgtt gcaaaagagg cattagactt gttgcaagta gacccgcttg
     2341 gtttggacga tgttgaccgc cgcgtgctca agaccattat cgaaaaatac aatggcggac
     2401 ccgtgggttt gaataccatt gctgcatcca ttagtgaaga acaagatacg atcatggatg
     2461 tggttgagcc ctacttgttg caacttggct ttttagaccg cactccacaa ggccgtgtag
     2521 cgaccaaatc tgcatatgag catttggggt atcagt
//
LOCUS       DAOQFT010000369         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25730_length_2555_cov_5.369600, whole genome shotgun sequence.
ACCESSION   DAOQFT010000369 DAOQFT010000000
VERSION     DAOQFT010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25730_length_2555_cov_5.369600"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLR93_09485"
     CDS             <1..563
                     /locus_tag="PLR93_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNH27240.1"
                     /translation="DPIPDSVIREILHTATLAPSAHNRQPWRFVVMTDKTSIAEFAKT
                     MAIEFRHVLEQDQLPPEEIQKRLNRSQERISGAPVVIIACVEMTEMEKYPDERRKKAE
                     YLIATQSVANAGMQLLLAAHAEGLGAVWICSPLFAQSAVQEALKLQPTWEPQAMFLVG
                     KPVEVPAVRERKPIEEISIFIDKKGN"
     gene            565..1479
                     /gene="cofD"
                     /locus_tag="PLR93_09490"
     CDS             565..1479
                     /gene="cofD"
                     /locus_tag="PLR93_09490"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNH27241.1"
                     /translation="MKIVAFAGGVGGAKLAQGLAQTLPPKDLTIIVNTGDDFEHLGLY
                     ICPDLDTVCYTLGGLANPETGWGRAGETWNTISNVEKLGGPAWFRLGDSDIATHLERT
                     RRLKEGKSLSEITKEFCKAWGIEATVLPMTDSPVRTVVDSAEGELAFQEYFVHRQCQP
                     RVKGFRFDGVEVAEPAQGVREALASADAIIFCPSNPWVSIDPILSVVKEVNKPVFAVS
                     PIIGGKTVKGPAAKMYSELGIEPSAYAVAKHYRTILKGFVLDTTDAAMEKEIQDLGFA
                     TLVTNTLMNQLTDRARLANDVLNFIRSL"
     gene            1482..2090
                     /gene="cofC"
                     /locus_tag="PLR93_09495"
     CDS             1482..2090
                     /gene="cofC"
                     /locus_tag="PLR93_09495"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNH27242.1"
                     /translation="MSLWAIVPVKPLRRGKSRLAGALTEEERAALNQELLERTLKTLT
                     SLKELDKVLVVSRDSHALTIARNHGAKTVQEDGQPHLNTALTRASVIAKVHATQGVLV
                     LPVDLPLLTTDDVLALIDRATKPPVVVIAPDRHHKGTNALLMVPAGQIEYDFGEGSFQ
                     RHSERALQSGARLEVVELPSLGLDLDDPEDLELIRKLEAQKV"
     gene            2205..>2555
                     /locus_tag="PLR93_09500"
     CDS             2205..>2555
                     /locus_tag="PLR93_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNH27243.1"
                     /translation="MTKQRVALYLQDSHDLRDGLDYAKYAEEKGFEAVWQAESRLVRD
                     AIVPMAGYAAVTNKIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRVICGLGAWWDPL
                     AANVGIDRKKPLTAM"
BASE COUNT          699 a          601 c          640 g          615 t
ORIGIN      
        1 cggacccgat tcctgattca gtcatccggg aaattctcca cactgccact ctcgctccat
       61 ccgcgcataa ccgtcagcct tggcgttttg tggtgatgac agataaaacg tctatcgctg
      121 agtttgcaaa gacgatggca atagagttca ggcatgtgct tgagcaagat caacttccgc
      181 ccgaagagat tcaaaaacgc ctaaatcgct cccaagaaag aatcagcggc gcaccagtcg
      241 tcattatcgc gtgtgttgaa atgaccgaaa tggaaaaata cccagatgaa cgccgtaaga
      301 aagccgaata tctgatcgca acgcaatcag ttgccaatgc cggcatgcag cttttactgg
      361 cagcgcatgc agaaggcttg ggcgccgtat ggatatgcag cccattattt gcacaatctg
      421 ctgtgcagga agcattaaaa cttcaaccta catgggaacc tcaagctatg tttttggttg
      481 gtaaaccggt agaggttccg gcagtgcgag aacgcaaacc gatcgaagaa atttcgatct
      541 tcattgataa aaagggaaac taaattgaaa atcgtagcat ttgcaggcgg ggttggcgga
      601 gcaaaactgg cacaaggact cgctcaaacc cttccaccaa aagacttaac cattatcgtc
      661 aacactggtg atgactttga acatttgggg ctttatatct gcccagacct tgataccgtt
      721 tgttacacct tgggcgggct tgccaacccc gagaccggtt ggggtcgggc tggcgagacg
      781 tggaacacaa tatccaacgt tgaaaagctt ggcggaccgg catggtttcg ccttggcgac
      841 tcggatattg caacccatct tgagcgcacg cgacgactaa aagaagggaa gtctctttca
      901 gagatcacaa aagaattctg caaagcttgg ggcattgaag cgacagtgtt gcccatgacg
      961 gattcgcctg tgcgaaccgt ggtcgactct gctgaaggcg aattggcatt tcaagaatat
     1021 ttcgtccaca gacaatgcca accgagagtg aaaggcttca ggttcgacgg agtcgaagtt
     1081 gctgaaccag ctcagggtgt gcgtgaggcg cttgcatctg cagatgcgat cattttctgc
     1141 ccatccaatc cgtgggtcag tattgatcct attctgagcg ttgtcaaaga agtgaataaa
     1201 ccggtttttg cggtttcccc catcattggt ggaaagacgg tgaaaggacc ggcagcaaag
     1261 atgtatagtg aacttggtat tgaaccgtca gcttatgcgg ttgccaaaca ttatcgcacc
     1321 atcttaaaag gatttgtgtt agacactaca gatgctgcca tggaaaaaga aattcaagac
     1381 cttggctttg caactcttgt cacaaacaca cttatgaatc agcttacaga tcgcgcacga
     1441 ctggcgaacg atgtgctaaa ctttatccgg agtttataaa aatgtcactt tgggcaattg
     1501 tgcctgtaaa accattgcgc cgcggaaagt cccgccttgc cggggcatta actgaagaag
     1561 aacgcgcagc attgaaccaa gaattactgg aacgcactct caaaaccctt accagtttga
     1621 aagagttaga caaagtactg gtcgttagcc gcgactcaca cgcattgacc attgcacgta
     1681 atcatggcgc taaaaccgtt caggaagatg gtcaaccgca tttgaacaca gcgttaacgc
     1741 gtgcatctgt gatcgcaaag gttcatgcaa ctcaaggcgt gttggttttg cccgtagatc
     1801 ttcccctgct tacaactgat gatgttttag ctttgatcga ccgagctacc aagccacccg
     1861 ttgtggtcat tgcaccggat agacatcata aagggacaaa cgctttgctc atggttccgg
     1921 ctggacaaat cgaatatgac tttggtgaag ggtcttttca aaggcatagc gagcgagcgc
     1981 ttcaatctgg cgcaaggtta gaagtggttg aacttccatc gcttgggtta gacttggatg
     2041 accctgaaga cttggaactc attcgcaagt tggaagcaca aaaagtatag acatcagttt
     2101 tgccacatgc tcgcaaaccc tatgaaattt gcgaaacatg tggcaaaatt ttttatattg
     2161 tcagacaatc aagtcgtatc attccacact cgaaggagaa tgtcatgacc aagcaacgtg
     2221 ttgctctgta cttgcaagac tcgcacgacc tacgtgatgg gctcgactat gcaaagtatg
     2281 ccgaagaaaa agggtttgaa gccgtatggc aagcggagag tcgcttagtg cgcgatgcga
     2341 ttgtgccgat ggcagggtat gccgctgtaa ccaacaaaat caaagtcggc tcgggtgtga
     2401 tcaataactg gactcgcaac attggtctgt tggctgcaac cttcttaact ttggatgatc
     2461 ttgctcccaa ccgtgtcatt tgcgggctcg gcgcatggtg ggacccacta gctgcgaatg
     2521 taggcattga ccgcaagaag cccttgactg ccatg
//
LOCUS       DAOQFT010000370         2553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25758_length_2553_cov_8.936749, whole genome shotgun sequence.
ACCESSION   DAOQFT010000370 DAOQFT010000000
VERSION     DAOQFT010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25758_length_2553_cov_8.936749"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLR93_09505"
     CDS             <1..375
                     /locus_tag="PLR93_09505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNH27244.1"
                     /translation="FPGGQKVDAHFRGHTIKTDQPNDNTAPMPFELFLASIGTCAGIY
                     VLGFCQQRNLPTAGIRIVQRNYSNPANGLVDKIDLEIQVPPNFPEQYHAALVRTAELC
                     KVKKTLEHPPVFDVMTKVVEAA"
     gene            460..927
                     /locus_tag="PLR93_09510"
     CDS             460..927
                     /locus_tag="PLR93_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNH27245.1"
                     /translation="MPYIIDGHNLIPKLGLRLDEPDDEMELVRLLQDFARIRRQQIDV
                     YFDGAPAGQAGMRKFGSIKAHFVRQGQTADSAIRRRLEEMGKAAKNWVIVSSDHEVQS
                     AARVHMAQAIRSEEFVKQIRIAFSTSTQTGASDSKLSAQEVEEWINLFSKKKE"
     gene            1162..2175
                     /locus_tag="PLR93_09515"
     CDS             1162..2175
                     /locus_tag="PLR93_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27246.1"
                     /translation="MMVSNDVVVQRSYVYFVRWCMAVVITMGVFACSYIPSLPSPTSF
                     TECIPSSYTANIYRPTPTPTFPVAVASTSIPNIVSTAQPMPVDIHLHFQKLIQEVSRW
                     TAITPFPLGDSSEVWVAITFLSPELLRTVSTTDLLSENPAGINLDIQVNNMLVEIAKR
                     EKLLFFLTAFKANTGGEATNSHTFQIDVENLKLINAKNLGIKPAYYDHNLGQKMEFPA
                     PGESSQLAQSLISNYGYLYYPLGVESDGNCVEVLESTFNTKIILEASSIIMDGKSTGP
                     YTWTIDYTYLLNTTNNPVPNATFIVNSAIESTDALPTVPVISDAFWEEYAKFIWGKLT
                     PKH"
     gene            2279..>2553
                     /locus_tag="PLR93_09520"
     CDS             2279..>2553
                     /locus_tag="PLR93_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27247.1"
                     /translation="MSTVFTFVPSREHEYPDHPERPGRLDILEPLLPSFGAEQIQAIR
                     ATEADVALVHPVKMIRSLQEACMEGPGVIDYAPTYVTQTSYEDALKAV"
BASE COUNT          711 a          629 c          539 g          674 t
ORIGIN      
        1 ttccccggcg ggcaaaaagt agatgcccac ttccgcggtc acaccattaa gacagaccaa
       61 cccaacgaca atactgctcc gatgccgttt gaattgttcc ttgcttctat tggtacctgt
      121 gcaggcattt atgtgctagg cttttgccaa cagcgcaacc tgcccactgc aggcattcgc
      181 atcgttcagc gcaattattc caacccggca aacgggttgg tcgataagat cgatcttgaa
      241 attcaggtac cacccaactt ccccgaacaa taccatgccg cactcgtgcg cactgcagag
      301 ttatgcaaag tgaagaagac tcttgaacat ccacctgtgt ttgatgtaat gaccaaagtg
      361 gtcgaagctg cctaagaaac cgaagataaa aagaccgcag acattcacag tctgcggtct
      421 ttttattcca aaacaactca agcacagtaa aatgaaccca tgccctacat tattgatgga
      481 cataacctga ttcccaaact tggattacgt ttggatgaac ccgatgacga aatggaactc
      541 gtgcgcctct tacaagactt tgcacggatt cggcgacagc agattgatgt ctactttgac
      601 ggcgctcccg ccgggcaagc tggcatgcga aaatttggca gcatcaaagc gcacttcgta
      661 aggcaaggtc aaaccgcaga ctcagccatc cgcaggcgat tagaggagat ggggaaagca
      721 gcaaaaaatt gggtcattgt ttcttcagac catgaggtgc aaagtgcggc acgagtgcat
      781 atggcacaag cgattcgctc ggaagaattt gtcaaacaaa ttcgcattgc cttctctaca
      841 agtacccaaa cgggtgcatc tgactcaaag ctttcggcgc aagaagtgga agagtggata
      901 aatctattta gtaaaaagaa agaatagtac ctttgtctta tacgttttta atgaaatgtg
      961 gacaaaaaag gtgtatattg tctgtacgac acctgccctc ccttttactg gtattctggt
     1021 gtcaaccgcc gctctggtca acatgccccc cccatgctga caggcggcgg taccttttta
     1081 acaatgccaa aatacacttg aagaaggtct gagaggtcaa tataatattt tctatccgat
     1141 caaatcaatg tggaggcggg cttgatggtt tcaaacgatg tagtggtaca gcgttcttat
     1201 gtgtattttg tacgatggtg tatggcggtt gtgatcacaa tgggggtttt cgcatgcagc
     1261 tatattcctt ctttaccatc ccccacctct tttacagaat gtatcccttc atcttataca
     1321 gctaatatct atcgtccaac accaacgccc acctttcctg ttgcagtagc atccacatcg
     1381 attcccaata ttgtttcgac tgctcagccc atgccagttg atatccatct gcattttcaa
     1441 aagttaattc aagaagtcag ccgctggacg gcgataactc ctttcccact tggcgattca
     1501 agtgaagttt gggttgcgat cacatttcta agccctgagc ttttacgaac cgtctctact
     1561 accgaccttt tgagtgaaaa cccagccggt atcaatttag atattcaagt taacaatatg
     1621 cttgtagaga ttgccaaacg cgagaaacta ttatttttct taacagcctt caaggcgaac
     1681 acaggtggcg aagccacaaa ctcgcacacg tttcaaattg atgtagaaaa tctaaagctt
     1741 atcaatgcca aaaacctcgg tattaaacct gcatactatg accataacct tgggcaaaaa
     1801 atggaattcc ccgcgccggg ggaatcttct cagctcgcac aatctttaat atcaaactat
     1861 ggatatttgt actatccatt aggcgtagaa tccgatggca attgtgtgga agtccttgaa
     1921 tcgaccttca acaccaagat catccttgaa gcatcctcca ttattatgga tggaaagtct
     1981 acgggaccat atacatggac gattgattac acctatctct taaacacaac gaataacccc
     2041 gtccccaatg caacctttat cgttaattct gcgattgagt cgacggatgc cttgcccact
     2101 gttcccgtca tctcagatgc gttctgggaa gaatacgcaa aatttatttg gggcaagctc
     2161 acgccaaaac actaattatg gctttttttc cacagcaggt tttaaaaaat gctcaaatct
     2221 gctgtgactt ctgtggctta agtttatgaa ttcaccaaca gggggctata attaaatcat
     2281 gtctaccgta tttacatttg tcccatcgcg ggaacatgaa tatccagacc accccgaacg
     2341 cccaggcagg ttggatattt tggagccttt gctaccctct ttcggcgcgg aacagatcca
     2401 agcgattcgg gcaaccgaag cggatgtggc tctcgtccac ccggtgaaga tgattcgttc
     2461 tctgcaagaa gcctgcatgg aaggacccgg tgtcattgat tatgcgccta cctatgtaac
     2521 gcagacttct tatgaagatg cgctgaaagc cgt
//
LOCUS       DAOQFT010000371         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25782_length_2552_cov_4.436924, whole genome shotgun sequence.
ACCESSION   DAOQFT010000371 DAOQFT010000000
VERSION     DAOQFT010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25782_length_2552_cov_4.436924"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLR93_09525"
     CDS             <1..286
                     /locus_tag="PLR93_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNH27248.1"
                     /translation="WVERITKAYASMFTSFVAHANRVGYEDGLHFWGGATIFDPTGEE
                     LAHGPYNEEAITYSELDLNQLRRTRARLPLLRDERTALVQKEMNRILARD"
     gene            279..1106
                     /locus_tag="PLR93_09530"
     CDS             279..1106
                     /locus_tag="PLR93_09530"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNH27249.1"
                     /translation="MIDINLSINTDLAREILSGFIKSEITRIGMSRAVIGLSGGIDSA
                     LSLALAVDALGKDNVLAVRMPYKASSKDSLDHAQLLIDQLGVPSKTIEITDMVEPLFK
                     LNPDMSKLRKGNIMARERMIVIYDQSEVFKGLVVGTSNKTEILLGYSTLFGDSANALN
                     PIGDLYKTQVRQLSRAMNIPSPIVDKPPSADLWEDQTDEGELGFTYSEADRLLYLLVD
                     QRYSPQEAIEAGFDEKFVNAVTTRIRRNQFKRMLPPIAKISNRTIGYDFLYLRDWGT"
     gene            1174..2463
                     /locus_tag="PLR93_09535"
     CDS             1174..2463
                     /locus_tag="PLR93_09535"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27250.1"
                     /translation="MHIPPSLKHKKFFYLWLGQLISITGTQMQLWALFWHINQIDKNP
                     IALGGIGVARILPVIIFSLIGGALADSVDRRKVMFATQSVAAVLALTLALFTQFGQVT
                     IWHIYIITALQAVVVAFDGPSRQALIPNLLPKEDLPNAFSMTFTAFQAGAVIGPALSG
                     FFIAGFGQEAVYYFNAISFVAVLVALLMIGDVPQAIGEKTAGISMESIKDGIRFILSK
                     PIIFSTMMVDFVATFFASASTLMPIIAKDVLDVGVVEYGYLSAASAVGAVAVALVISQ
                     VKEIRKQGLVFVVAVIIFGIATIIFGITRSFIVAWLAIAVTGGADGVSTIVRNTIRQL
                     QTPDHVRGRMTSINQIFFMGGPQLGEIEAGAVAQFFGAPIAVITGGIGCIVGTLLIIW
                     KWPQLREYNGDEPIEAGGSFPSPSTGASIIANRVRKQ"
BASE COUNT          614 a          624 c          626 g          688 t
ORIGIN      
        1 ctgggtggaa cgcatcacca aagcctatgc cagtatgttc acctcgttcg tagcgcatgc
       61 caaccgagtg ggttatgaag atggactgca cttctggggt ggtgcaacga tctttgaccc
      121 aacgggggaa gaactggcgc atggacccta taacgaagaa gccatcacat actctgaatt
      181 ggatttgaat caacttcgcc gcacacgcgc ccgcttgcct ttgttgcggg atgaacgcac
      241 ggctctcgtc caaaaagaaa tgaacaggat tctcgcccgt gattgacatt aaccttagca
      301 tcaatacaga cctggctcgc gagattctct caggtttcat caaatctgaa attacccgca
      361 ttggaatgtc acgcgcggta atcgggcttt ctggtggtat tgactcggcg ctttctcttg
      421 cgcttgctgt tgatgcgctc gggaaggata atgtccttgc ggtgcgcatg ccgtataaag
      481 cgtctagcaa agattcgttg gatcacgctc aattattgat cgaccaattg ggtgtgccca
      541 gcaaaaccat tgaaattacc gacatggttg agcccttgtt caaactcaat cccgatatgt
      601 ccaaactgcg caaaggcaac atcatggcgc gtgaacggat gatcgtgatc tacgaccaaa
      661 gcgaagtgtt caaagggcta gttgtcggta caagcaataa gactgaaatc cttttggggt
      721 acagcactct cttcggtgac tcggcgaatg ctcttaatcc cattggagat ttgtacaaga
      781 cgcaagtgcg tcaactttca agggcgatga atattccatc tccaattgtc gataaaccac
      841 cttccgctga tctatgggaa gaccaaaccg acgaaggcga attaggcttt acctattctg
      901 aagcggacag gctcctctat cttttggtgg atcagcgcta cagtccgcaa gaggcgattg
      961 aagctggctt tgatgagaaa tttgtcaacg ccgtcacaac acgcattcga cgcaatcaat
     1021 ttaaacgtat gcttccgccg atcgccaaga tcagcaatcg caccatcggc tacgacttct
     1081 tatatctccg cgattgggga acataaaaaa taatggagta ggggcagggc ttatgccacg
     1141 aagtggtatc gcctcgcccc tacaaataat aaaatgcata ttcctccctc tctaaaacat
     1201 aaaaaattct tttatctttg gctcggacaa ctcatctcca tcaccggaac ccagatgcaa
     1261 ctctgggctt tattttggca tatcaaccag attgacaaaa accccatcgc gcttggtggc
     1321 attggtgtcg cgcgtatttt gcccgtcatc atcttttctt tgatcggtgg tgcactagca
     1381 gactcagtgg atagacgtaa agtgatgttt gccacacagt ctgttgccgc ggttcttgct
     1441 ctgaccttgg ctttgttcac acaatttggg caggtcacca tctggcacat ttacatcatt
     1501 actgcattgc aagcggtggt cgtggcgttt gatggtccat ctcgtcaggc gttgattccg
     1561 aacttgttac ccaaagagga tttgccaaat gccttcagta tgacattcac agcctttcaa
     1621 gctggcgctg tgattggacc cgccttgagt ggcttcttca ttgctggctt tggtcaggaa
     1681 gcagtttatt acttcaatgc gatttcattt gtagcagttt tggtcgctct tctgatgatc
     1741 ggagatgtcc ctcaagccat tggcgaaaag acagcgggca tcagtatgga atctatcaaa
     1801 gacggcattc gctttatcct ctccaaaccg attatctttt ccaccatgat ggttgatttt
     1861 gttgcgacct tctttgcctc tgccagcacc ttgatgccca tcattgccaa agatgtattg
     1921 gatgtaggtg tagtggaata tggttacctc tccgccgctt cggcggtggg agcagtggca
     1981 gtagcattgg ttatttcaca ggttaaagag attcgcaaac agggactggt gttcgtggtt
     2041 gctgtcatta tctttggaat tgcaacgatc atttttggga ttacaaggtc atttatcgtt
     2101 gcatggctgg caattgccgt caccggcggt gccgatggag tcagcaccat cgtacgcaat
     2161 accatccgtc agttgcaaac gccggatcac gtccgcgggc ggatgacgag tatcaaccag
     2221 atcttcttca tgggcggacc gcagctcggc gagatcgaag ctggagccgt ggcgcagttc
     2281 tttggcgcgc ccattgccgt cattactggc ggcattggtt gtatcgttgg aaccttgctg
     2341 attatttgga agtggcccca actacgagaa tacaatggtg atgagcccat agaagcaggt
     2401 gggtcattcc caagtccatc tactggggct tcgatcatag ccaacagagt gcggaaacag
     2461 taaacaaaga gacagttacc tcgcaggtga ctgtctcttt tatttcgggg gattctctat
     2521 caagcttttc atctcagcca acacggcttg at
//
LOCUS       DAOQFT010000372         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_25893_length_2546_cov_7.559615, whole genome shotgun sequence.
ACCESSION   DAOQFT010000372 DAOQFT010000000
VERSION     DAOQFT010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25893_length_2546_cov_7.559615"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLR93_09540"
     CDS             <1..537
                     /locus_tag="PLR93_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNH27251.1"
                     /translation="GDKVLINGATGGVGTFAVQIAKAMGAEVTGVTSTKNVELIRSLG
                     ADTVIDYSKEDFTQTNKQYDILLDIAGSRTWKEYRKVLKPKANFVIVGGQKGNKFIGP
                     LANALRLRVSALGASQTVAFFIANFSRDDLNLLRELVERGKVKPVVEKVYPLAQAADA
                     MKHLGTGHARGKIVLKVK"
     gene            618..2168
                     /locus_tag="PLR93_09545"
     CDS             618..2168
                     /locus_tag="PLR93_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH27252.1"
                     /translation="MEITYFEFVKHLIRFNRNVYLDHNATTPVSEHVRNKMDQALKYH
                     YGNPSSFYGSGRKSAGLVEEARGHVANAIHAEPYEVIFTACATESNNAVLKSVSAHFY
                     PQKKKIISTPIEHPSVLNTLAYLETQGVVVEFCPVDNKGRIQLTHLEKMVDNDTFLVC
                     CILANNETGVLQDIQAVTKIAKSHGALVLTDCVQALGKIPIDVHTWDVDYASFSAHKL
                     YGPKGVGALYVKQGSPFTPLLHGGHQEGGLRAGTESTHNIVGFGSACQQVDKLLAHVE
                     EVRALKHDLIQRLKEIKPDCVINSPEVDSECLPNTLSIRFPGVENSGLMGMLDYRGIA
                     VSAGSACSTGEDTPSHVLKAIGLSDQAARETIRISLGHDTSARDIQYTANVIRDYLEG
                     RISFVNMLAPAQLNETILFSDDTFILDVRPSDDRAKFKGIPNAHEVNPLFVENYIKQL
                     PRDKQIIVYCPGGGLSVMISYFLKAKGFKRITNLRGGLDAWRKRRADLYGKYAGQNVT
                     VLEPDAKR"
     gene            2299..>2546
                     /locus_tag="PLR93_09550"
     CDS             2299..>2546
                     /locus_tag="PLR93_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27253.1"
                     /translation="MNKGPFIVLRVLGILLILGLIAGAGAFGYKAGLAQGIQQAPEVA
                     AAIEKAAENGQVAMPGAPMMYGYGYPHMYGFGHPHFFNP"
BASE COUNT          701 a          597 c          630 g          618 t
ORIGIN      
        1 ggggataagg ttctcatcaa cggcgcgacg ggaggcgtgg gcacgttcgc agtgcaaatt
       61 gcaaaggcaa tgggagccga agtgaccggt gtaaccagca cgaaaaatgt tgagttaatc
      121 cgctcacttg gcgcggatac cgttattgac tacagcaaag aggatttcac ccaaactaat
      181 aagcagtatg acattctctt ggacattgcc ggaagccgga cgtggaaaga atatagaaaa
      241 gtcctcaaac ccaaagcaaa ctttgtcatt gttggtgggc agaagggcaa taagtttatt
      301 ggtccgctgg caaatgcgct tcggttgaga gtttcggcat tgggtgccag ccaaactgtt
      361 gctttcttca ttgccaattt cagccgtgac gacctcaatc ttttacgaga gctagttgaa
      421 aggggaaaag taaaacccgt cgtggagaaa gtctatccgc tggcgcaggc agccgacgcc
      481 atgaagcatt tggggacggg gcatgcacgc ggcaagatcg tgctcaaggt gaaataagag
      541 ggcatccatt accaactatt acttcggaac aacgtatact gagagtaacc gtatcatagc
      601 tttatcccgg agtctcaatg gaaatcacat attttgaatt cgtaaaacac ctcatccgct
      661 tcaaccgcaa cgtttatctt gaccataatg caacgacacc ggtcagcgaa cacgtacgca
      721 ataaaatgga tcaggcactg aaatatcatt atggtaaccc ttcgtcattt tatggcagtg
      781 gaagaaaatc agctgggtta gtagaagaag cccgagggca tgtggcaaat gccattcatg
      841 ccgagccgta tgaggtgatt tttacagcgt gcgctactga atcaaataat gcggtactga
      901 aatctgtttc agcgcacttt taccctcaaa agaaaaagat catttcaaca ccgattgaac
      961 acccttctgt gttgaataca ttggcatatt tggaaacaca gggcgtggtt gtcgaatttt
     1021 gcccagtgga taacaaaggg cgcatccaac tcactcacct cgaaaaaatg gttgataacg
     1081 acaccttttt agtttgctgt attctagcca acaatgaaac cggagtgctt caagatattc
     1141 aggcggttac aaaaattgct aaatcacacg gagcgctggt gttgacagac tgcgtgcaag
     1201 ccttgggaaa gatcccaatt gacgtccaca cttgggatgt agattacgcc tctttttcgg
     1261 ctcacaagct gtacggtcca aaaggagtcg gagcgttata cgtcaagcag ggaagcccat
     1321 ttacaccact gctgcatggt gggcatcaag aaggcgggct acgggcaggg acagaaagca
     1381 cccacaatat cgtcggcttt ggctccgcct gccaacaagt ggacaaactt ctagcacatg
     1441 ttgaagaagt ccgtgcattg aaacatgatt tgattcaacg tctcaaagaa attaaaccag
     1501 attgcgttat caattcgccc gaggtggatt cggaatgtct accgaacaca ctcagcatcc
     1561 gtttccccgg tgtggaaaat tccgggctca tgggcatgtt ggattatcgt ggaatcgccg
     1621 tctctgctgg ctcggcgtgc agtacaggtg aagatacgcc atcgcatgtt ttgaaagcaa
     1681 tcggtttgtc tgaccaagcc gctcgcgaaa cgattcgcat cagtctcggg cacgatactt
     1741 cggcgcgaga tattcaatat acagcaaacg taattcgaga ttatctcgaa ggtcggattt
     1801 cgttcgtgaa tatgctcgct cctgcgcagc tgaacgagac aatccttttt agcgatgaca
     1861 cgtttattct cgatgtgcgt ccctctgatg accgggcaaa gttcaaggga atacccaatg
     1921 cacatgaggt caacccgctg ttcgttgaaa actatataaa gcaactcccc cgcgataagc
     1981 agattatcgt ctattgcccg ggcggaggtc tttcggtcat gatctcttat ttcttgaaag
     2041 caaaagggtt taaacgcatc accaatctgc gcggcgggct tgatgcgtgg cgcaaacgac
     2101 gggctgactt gtatggaaaa tatgcggggc aaaacgtgac agttcttgag ccagatgcta
     2161 aacgttaagc aagaggcgct aataattttc ctacattccc tccacaatcc ctcacctgtc
     2221 catccataat ccctccacaa tgggttgata catttagacg cgtaaggaca aatcaaacaa
     2281 ttaccattgg aggtaaaaat gaataaaggt ccgtttattg ttttacgcgt tctcggtatt
     2341 ctgctcatcc tcgggctgat cgcaggcgca ggggcattcg ggtataaggc tggtttggca
     2401 caagggattc aacaagctcc tgaagtggcc gccgccattg agaaagccgc agagaatggt
     2461 caggttgcta tgcccggcgc acccatgatg tacggatatg gctacccgca tatgtacggt
     2521 tttggtcacc cgcacttctt caatcc
//
LOCUS       DAOQFT010000373         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_26078_length_2536_cov_6.103184, whole genome shotgun sequence.
ACCESSION   DAOQFT010000373 DAOQFT010000000
VERSION     DAOQFT010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26078_length_2536_cov_6.103184"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLR93_09555"
     CDS             complement(<1..938)
                     /locus_tag="PLR93_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH27254.1"
                     /translation="MKEQLSAIHPELQGPAKFAPKLNYNKFVVWLLNTLMRLAPSPKA
                     PADLHIENIFITASDGNQVRVRVYKPKAAKEALPALLWLHGGGYLIGNPEQDENTCIQ
                     YVRELGIQIVSVDYRLAPKHPFPAGLDDSYTALKWMATQANELGIDNKRIAIGGASAG
                     GGLAAALVQFAHDKQEVQPVFQLLVYPMLDDRTALRTDLNDINITWNQPSNRFGWSSY
                     LGKECGSDSVSEYSVPARRENLAGLPSAWVGVGDLDLFYTEDLLYAQRLQAAGVDCEV
                     VVVPGAFHGFDAFTSQASIVQEFRKSQIAALRKSFYG"
     gene            complement(973..>1331)
                     /locus_tag="PLR93_09560"
     CDS             complement(973..>1331)
                     /locus_tag="PLR93_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNH27255.1"
                     /translation="DVFSSSLPWLVLENNDEVIGYAYATKWRARPAYKNSVETTIYLA
                     KDSGGKGFGKLLYSTLISTLKSSGFHTAIGGIALPNEASVGLHESLGFKKVAHFHEVG
                     YKFDTWIDVGFWQIML"
     assembly_gap    1332..1431
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1432..1832)
                     /locus_tag="PLR93_09565"
     CDS             complement(<1432..1832)
                     /locus_tag="PLR93_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27256.1"
                     /translation="MPKQRDSRFITIRRGGTLQDSDHHLLALWAADCAEHVLHLFENI
                     CPQDDRPRQAIESARAWTRGEISMSQSRAAGGHAMAAARALSGAARHAAFAAGQAGVV
                     AHVAAHELGAAAYAIKAVQAAALEGESKEAGR"
     gene            complement(1860..2141)
                     /locus_tag="PLR93_09570"
     CDS             complement(1860..2141)
                     /locus_tag="PLR93_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNH27257.1"
                     /translation="MSFQAYLDKVEELTGKAPKEFVAEAKKKKLTTHKEILDWLKTDY
                     GLGTGHARAIAHVILHGAEFTVKQTTGTHRDASGTLNLNGKKADKKKKS"
     gene            complement(2168..>2536)
                     /locus_tag="PLR93_09575"
     CDS             complement(2168..>2536)
                     /locus_tag="PLR93_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27258.1"
                     /translation="TVQTLAGMSMFVIADVTSPKSTPLELEATAKQFKIPFIPIIDIS
                     VDPRPFAMLVDLQKSFHWVLPTLQYQTKEDLLDDENLKTYIIDRAVAKREELRVAKSQ
                     EPEMLLVRKKGTSIPPATAS"
BASE COUNT          596 a          612 c          568 g          660 t
ORIGIN      
        1 ccatagaaag attttctcaa tgccgcgatt tgagattttc gaaattcctg aacgatagat
       61 gcttgcgaag tgaatgcatc aaagccatga aatgcccccg gcaccacaac tacttcgcaa
      121 tcaacaccgg ctgcttgcaa gcgttgagcg tagagcaaat cttcagtgta gaaaaggtcc
      181 aagtcgccta ccccaaccca ggcactcgga agacccgcga gattttcacg tcgggctggc
      241 acagaatact ctgacacact gtcacttccg cattctttgc caagatagga agaccagccg
      301 aatcgattgc tcggttgatt ccaagtgata ttgatatcat ttaaatctgt ccgaagcgcg
      361 gttctatcat ccaacatggg gtacaccaaa agctgaaaga ccggttgcac ttcttgctta
      421 tcgtgcgcaa actgcaccaa tgctgcagcc aacccaccgc ctgcactcgc accaccaatg
      481 gcgatgcgct tgttatcgat accaagttca tttgcttgag ttgccatcca ctttagcgcg
      541 gtgtaactat catccaaacc ggcggggaag ggatgcttgg gagcaagccg ataatcgaca
      601 gacacaattt ggattcccaa ctcgcgcacg tattggatac aggtattttc atcctgctcg
      661 ggattgccaa tgagataccc gcccccatgc agccataaca aagcgggtaa cgcctctttc
      721 gccgccttcg gcttgtatac ccgtacccga acttgattgc catctgaagc tgtgatgaag
      781 atgttctcaa tatgcagatc tgcaggtgct ttgggcgacg gtgccaaacg catgagggta
      841 ttcaataacc aaaccacaaa cttgttgtag tttaactttg gcgcaaactt tgccggacct
      901 tgtaactcgg ggtggatagc tgagagttgt tctttcatat aaggttatct gtttacttta
      961 gaaaaaagaa gctcataaca tgatctgcca aaagcctaca tcgatccatg tatcgaattt
     1021 gtatccaact tcatggaagt gagccacttt tttgaacccc aaggactcat gcagaccaac
     1081 actagcttca ttcggcaaag cgatgccgcc tattgcagtg tgaaatcctg aagacttaag
     1141 agttgagatc aatgttgagt agagcaattt accaaatcct tttccacctg aatctttggc
     1201 tagataaatc gttgtttcga cagagttttt ataggcaggt cttgcccgcc attttgtggc
     1261 atatgcatac ccgatcactt catcattatt ttcaagcacg agccatggca aagaggaaga
     1321 aaatacatct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgccccgct
     1441 tcttttgatt caccctccaa tgccgctgct tggacggctt tgatggcata ggcggcggct
     1501 cccagctcat gggcagctac atgcgccacc accccggctt gacctgcggc aaaagcggca
     1561 tgccgagccg caccactcaa ggcacgcgcc gcagccatgg catgtccgcc tgccgcccgg
     1621 ctttgtgaca tgctgatctc accacgcgtc catgcccgag cagactcaat tgcctggcgc
     1681 ggacggtcgt cttgtgggca aatattctca aacaagtgca agacatgctc agcacaatct
     1741 gctgcccata atgccaatag gtgatggtct gaatcttgta aggtgccgcc gcgtcgtatg
     1801 gtgatgaatc tagagtctcg ttgtttaggc aatatcatgg cttggtttaa tgccgttgtt
     1861 taagactttt tctttttatc ggcttttttg ccattcaagt taagcgtgcc agaggcatcg
     1921 cgatgcgttc cggtggtttg ttttacggta aattcagcac catgcaaaat cacatgtgca
     1981 atggctctgg catgcccggt acccagaccg taatccgttt ttaaccaatc gagaatctct
     2041 ttatgtgttg tcagcttctt tttctttgcc tcagccacga attctttcgg ggctttgcct
     2101 gtaagttctt caactttatc taaataagct tgaaacgaca tggttttctc ctgtaagggt
     2161 taacgcctca acttgctgtt gcaggcggga tggatgtgcc ttttttgcga acgagcagca
     2221 tttcaggctc ctggcttttt gcaactcgta actcttcgcg ttttgcaacg gcgcgatcaa
     2281 tgatataggt ttttagattc tcatcatcca acaggtcttc tttggtttga tattgaaggg
     2341 ttggcaaaac ccaatggaaa gatttttgca aatcgaccag catggcgaac ggacgtgggt
     2401 ctacgctgat atcgataatg ggaatgaaag ggatcttgaa ttgtttggcg gtggcttcca
     2461 gttctaatgg ggtcgacttc gggctggtca catcggcgat gacgaacatg ctcatacctg
     2521 ccagggtttg aaccgt
//
LOCUS       DAOQFT010000374         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_26095_length_2535_cov_4.658468, whole genome shotgun sequence.
ACCESSION   DAOQFT010000374 DAOQFT010000000
VERSION     DAOQFT010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26095_length_2535_cov_4.658468"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLR93_09580"
     CDS             <1..656
                     /locus_tag="PLR93_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27259.1"
                     /translation="WTEAKEIFKKKTMWFVFLQGFAGVFPWNVITFFFFSYLEKERGY
                     DGNSILFTMAPVILILASGYFIGGWAGDLAFKRTNKGRIIVSSVGVLLGAVFMYFAIT
                     TPTEARNQFFVLMCLTAVFMPLSSANVIATVYDVTVPEVRSTAQAVEYFVENAGAAFA
                     PIITGLIADAIDLKTAILLICTVSWVLCFFFYLGAIFTIDKDTHDLRNQMAERAKSM"
     gene            668..1351
                     /locus_tag="PLR93_09585"
     CDS             668..1351
                     /locus_tag="PLR93_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27260.1"
                     /translation="METQQIQEDQARQLVQNTISGQETIRWVGTPDPIHAIRKTWSAS
                     IFGLIFGGFIYYIFSNIIEINNRGPVYMQPSPSLKMVTSLVLVVFIIVTLRFVFAPLW
                     EYLIAKGKVYAITDQRALIIEQFPSDKQTSYYPNDIKYVQIRGKGIGDIIFDSQTKIV
                     TDYENIPGSDRQRMVRRQVKVNIGFFSISLPSPASELLRQMKASVQPPEAKQKAAYPT
                     VHPADRFRQ"
     gene            1532..2080
                     /locus_tag="PLR93_09590"
     CDS             1532..2080
                     /locus_tag="PLR93_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH27261.1"
                     /translation="MDDATGIIFKALRFSAEKHSEQRRKDAKSSPYINHPIQVAETLW
                     SIGGVRDANLLAAAILHDTIEDTNTTPAEIQTLFGEDVLSLVLEVTDDKSLPKDVRKK
                     MQIENASHKSPRAKLLKLADKLCNIYDITHIPPTTWPLKRKQDYLLWSEKVVHGLRGT
                     NKELEARYDEVLQEGKHLLKMG"
     gene            2186..>2535
                     /locus_tag="PLR93_09595"
     CDS             2186..>2535
                     /locus_tag="PLR93_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNH27262.1"
                     /translation="MTIIVADTTCGLPREMLTERGITLIPQVVIFGEESFHDDKDLDT
                     ASFLQKLKAFKSLPKTAAPEPPLYYPVFEEAQKKGESVVVVAPTGKASGTVRSAQTAA
                     KEFPEVDIRVVDTQT"
BASE COUNT          683 a          677 c          591 g          584 t
ORIGIN      
        1 cgtggacaga ggcaaaagaa atcttcaaga agaaaacaat gtggtttgtg ttcttgcagg
       61 gttttgcggg tgtcttcccc tggaatgtga tcaccttctt cttcttttct tacttggaaa
      121 aagaacgcgg gtacgatgga aacagtatcc tcttcaccat ggcgcccgtg atccttatcc
      181 tcgccagcgg atatttcatt ggcggctggg ctggcgacct ggcctttaaa cgcaccaaca
      241 aaggacgcat catcgtttca agcgtcggcg ttttattggg tgcggtcttt atgtactttg
      301 ccatcaccac acccaccgaa gcccgcaacc agttctttgt attgatgtgc ctgacggctg
      361 tgttcatgcc gctttcatcg gcaaacgtga ttgccactgt atacgatgtg accgtgccgg
      421 aagtccgctc gacggcgcag gcggtggagt attttgttga aaatgcaggt gcggcgtttg
      481 ctcccatcat caccgggctg atcgcggatg ccatcgacct caagaccgcc atcctgctca
      541 tctgtacagt gtcctgggtt ttatgtttct tcttctacct cggtgcgatt tttaccatcg
      601 acaaggatac tcacgacctg cggaatcaaa tggctgaacg cgccaaatcc atgtaagcag
      661 aggccctatg gaaactcagc agattcagga agaccaagcc cgccagcttg tgcaaaacac
      721 catctccggg caggagacca tccgttgggt cggaacgcca gacccaattc acgccattcg
      781 caaaacctgg agtgcaagta tatttgggct gatctttggc ggttttattt actacatctt
      841 ttccaacatt atagaaatca acaacagggg accggtttac atgcagccaa gcccaagcct
      901 aaagatggtg acgtctctgg tcttggtcgt attcatcatc gtcaccttgc gctttgtctt
      961 cgccccgctc tgggagtatc tgatcgccaa aggcaaggtc tacgccatca ccgatcaacg
     1021 cgcactcatc atcgaacaat ttccgtcgga caagcagaca tcttattatc ccaacgatat
     1081 caaatatgtt caaatccggg gcaaagggat cggcgatatc atcttcgact cacaaacaaa
     1141 aattgtgacc gactatgaaa atatacccgg ctccgaccgc cagcgaatgg taagacgcca
     1201 ggtgaaggtc aatattggct tcttcagtat ttcccttcca tccccggcaa gcgaattact
     1261 gcgacagatg aaagccagtg tccagccgcc ggaagcaaag caaaaggccg cgtaccctac
     1321 agtacatccg gcagatcgat ttagacaata aacctgccca tccactaatt gacatagaac
     1381 cgcctccgac ttcgccggag gcggttaccg ttccatagat attttcttcg ccgtgaaccc
     1441 tccacttcac acaggatgtc gcaatttgtg agagaataaa gtcactgaaa cccgatcaaa
     1501 atgagaatat ctaacaaagg aatttaaatt tatggatgat gcaaccggaa ttatcttcaa
     1561 agctctgcgt ttctcagccg agaaacatag tgaacagcgc cgcaaggatg cgaaatcctc
     1621 gccgtatatc aatcatccca ttcaggtggc tgagacgtta tggagcattg gcggagtgcg
     1681 tgacgcgaac ctgctcgccg ctgccatatt gcacgacacc attgaagata ccaacaccac
     1741 accagccgag atccaaaccc tctttgggga ggatgtgctc tccctggtac ttgaagtgac
     1801 cgacgacaaa agcctgccca aggacgtccg caagaaaatg cagatcgaga atgcctcaca
     1861 caaatcgccc cgggcaaaac tcctcaaatt ggccgacaag ctttgcaaca tctacgacat
     1921 cacccacatc ccacccacca cctggcccct caaacgcaaa caggactatc tgctctggag
     1981 tgagaaggtc gtacacggcc tgcgcgggac aaacaaagaa ttggaagctc gttatgatga
     2041 ggttttacaa gagggtaaac atctattgaa aatgggataa ccggcaccga ataatctgtc
     2101 ctttgtaata gaattttcag accgtttgac agttaatcta cctgggaata ggggtctaca
     2161 ataaatgaac ggagaactga ttcccatgac gatcatcgtt gctgatacca cctgcgggct
     2221 gccgcgggaa atgctaaccg aacggggaat tacgcttatt ccacaagtcg tcatctttgg
     2281 ggaagagtct tttcatgacg ataaagacct tgatacagcc tcatttttgc aaaaattaaa
     2341 agccttcaaa tccctgccca agaccgccgc gccggaaccg cccctgtatt accccgtttt
     2401 tgaagaggcg cagaaaaaag gggaaagcgt tgtggtggtt gcaccaaccg gcaaagccag
     2461 cggaactgtc cgctcggcac aaacagcggc aaaggaattc ccagaggtgg atattcgcgt
     2521 ggtggataca caaac
//
LOCUS       DAOQFT010000375         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_26165_length_2531_cov_5.282310, whole genome shotgun sequence.
ACCESSION   DAOQFT010000375 DAOQFT010000000
VERSION     DAOQFT010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26165_length_2531_cov_5.282310"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLR93_09600"
     CDS             complement(<1..312)
                     /locus_tag="PLR93_09600"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNH27263.1"
                     /translation="MQLYFIRHGQSINNANSENEGYQEHSDPYLTEIGKKQAQLTAQY
                     LKERQTLSDPESWNSQNQFGFGFTHIYCSLMERATETASYTARAIGNIPFEAWVDIYE
                     EG"
     gene            complement(387..716)
                     /locus_tag="PLR93_09605"
     CDS             complement(387..716)
                     /locus_tag="PLR93_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNH27264.1"
                     /translation="MKKQKALVFNWEAAKAARNEEKYGVSFEEAKVIFTDKYAVYHDD
                     VEHSQKEKREIVIGRNFNTKRHLTCFFTRVDGVIHIIGARLSTHREREEYKENSDEAK
                     RIIYQIK"
     gene            complement(754..1539)
                     /locus_tag="PLR93_09610"
     CDS             complement(754..1539)
                     /locus_tag="PLR93_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27265.1"
                     /translation="MSLGKKIAIGFGVAFILLCIAAIAFVNWSNSDAGQATFAEVGTQ
                     QAVASATGAVQTQQASGTATAVWAEIDARLQNATLIYENTFDEGTPLVNKFGEDLKPY
                     YRDGIPEVIVAWNGSTTLPIGQSVSDFVAEVDCMANGAFCGIAYDINPGKDDFDVYFA
                     SGIGTADRFFFSDLYASLPSTNWDGENFPASPAEGFLHRIRVERFGQVMRFYVNGQLM
                     EERLLDAPGSASGDVGFYFGKASEESGGIQTVQFDNFRVWELP"
     gene            complement(1684..>2531)
                     /locus_tag="PLR93_09615"
     CDS             complement(1684..>2531)
                     /locus_tag="PLR93_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNH27266.1"
                     /translation="VSYNGIRWTDFTSDMFEQRLEVQLTLKNKIITALGGEALDFKKV
                     RDEYRGYYEKVKPYLKELFPLVQEGLKTNKTFLLEQAMGTFLDTDWGTYPFVTASTTI
                     PSAASAGLGIPPKYINNVIGVTKAYTTRVGAGPLPTEILDTKSAEYEQFGEVAATTGR
                     IRRVGWLDLEIIKTAVQLSGMTEICLTKLDVLSGLKELQVCVGYKIDGKSVTYPEVDA
                     YGLDKVECVYESFPGWSEDISKAKSFEELPLNAQKYVKMIEDAAGVPVKWIGVGPARD
                     ETIRR"
BASE COUNT          610 a          618 c          601 g          702 t
ORIGIN      
        1 gccttcttcg taaatatcca cccaggcttc gaaggggatg ttgccgattg cacgtgcggt
       61 ataggaggca gtttcggtag cgcgttccat caggctgcaa tagatgtggg tgaagccaaa
      121 gccaaattga ttctggctat tccatgattc gggatcgctg agcgtttgac gttctttgag
      181 atattgtgcc gtgagttggg cttgtttttt gccgatctca gtgagatacg ggtcggaatg
      241 ttcttgataa ccttcattct ctgaattggc gttgttgatc gattgtccgt gacggatgaa
      301 gtagagctgc atggttttcc tttcgagaaa aaacaacagt catttcagcg aaatgactgt
      361 tgtttgaaat ttatgttgac ctgattttac ttgatttggt aaatgattct tttggcttcg
      421 tcggagtttt ctttgtactc ttctcgctct cgatgcgtgg acaatcttgc gccgatgatg
      481 tgaatgactc catctacacg agtgaagaag caggtcaggt gacgtttggt attaaagttg
      541 cgtccaatca caatttcgcg cttctctttt tgggaatgtt ctacatcatc gtgatagacc
      601 gcgtatttat ctgtaaaaat gactttggct tcttcgaagc tgacaccata tttttcttcg
      661 ttacgggcgg ctttggcagc ttcccagttg aatacaaggg ctttttgttt tttcatgtac
      721 tcgagtatag catgacagta ggggtgggta aatttacggc aattcccaaa cgcggaagtt
      781 atcgaactgc acggtttgaa tgccaccgct ttcttcagat gccttcccga agtaaaagcc
      841 cacgtcacca gaggcagacc cgggcgcgtc gaggaggcgt tcttccatca actgcccgtt
      901 gacatagaaa cgcataacct gcccaaagcg ttccacgcgg atgcggtgta aaaatccttc
      961 ggcgggagaa gcggggaagt tttctccatc ccaattagtg gagggcaagg aggcgtacag
     1021 atcgctgaag aagaagcgat cggcggtgcc aatgcccgat gcgaaataga catcgaagtc
     1081 atctttgccg gggttgatat cgtatgcgat accgcagaaa gccccatttg ccatgcagtc
     1141 tacttcggcg acaaaatcgg aaacggactg accgatgggt aaagtcgtgg acccgttcca
     1201 ggctacgatg acctctggga tgccatcgcg ataatagggt ttaaggtctt caccaaactt
     1261 gttgacaagt ggcgtgcctt catcgaaggt attttcgtaa ataagtgtgg cgttctgtaa
     1321 acgggcatcg atctctgccc aaactgctgt ggcagtgcca gaagcctgct gggtttgcac
     1381 cgcgcctgtg gcgctggcaa ccgcttgctg tgtgccaacc tcggcaaaag ttgcttgccc
     1441 ggcatctgaa tttgaccagt tgacaaaggc aattgcagcg atacatagca atataaaggc
     1501 aactccgaac ccgatggcga tcttctttcc aagtgacata aagatattct cctaaaatgt
     1561 aatatcaaca attatattga atctgctgat tttgtttgcg gtacaaatct cctataaaaa
     1621 atacaggtga cagttaatca caaagtaact gtcacctgtt tcttatttgc tgaccaccaa
     1681 tttctatcta cgaatcgtct catcccttgc cggaccgacc ccgatccact tgaccggcac
     1741 gcctgccgca tcttcgatca tcttcacata cttctgcgcg ttgagaggta actcttcaaa
     1801 ggattttgcc ttgctaatgt cttcactcca gccgggaaat gattcataga cgcattcgac
     1861 cttgtctaaa ccgtaggcat ccacttcagg gtatgtgacc gacttcccat caattttata
     1921 gcccacacaa acctgcaatt cttttagtcc gctcaacacg tctaacttcg tcaagcaaat
     1981 ctcggtcatg ccgctaagtt gcacagcggt cttgataatt tccaagtcca accagcccac
     2041 gcggcgaatg cgacctgtgg tggcggcaac ttcgccaaac tgttcatatt cggcgctctt
     2101 tgtatcaaga atttcggtcg gcaacggtcc agctcccaca cgggtggtgt acgccttggt
     2161 cacaccgatg acattgttga tgtacttggg cggaatgcca agcccggcag aagccgccga
     2221 agggatggtg gtggatgctg tcacaaatgg atacgtgccc caatccgtgt ctaagaaagt
     2281 tcccatggct tgttcaagca aaaaggtttt gttcgttttc aaaccttcct gcaccagcgg
     2341 aaacaactcc ttgagatacg gcttgacctt ctcgtaatat ccgcgatatt catcgcgcac
     2401 ttttttgaag tctaacgctt cgccgcccaa cgccgtaatg atcttattct tcaaggtcaa
     2461 ttgcacttct aaacgctgct cgaacatatc actcgtaaaa tccgtccagc gaatgccgtt
     2521 gtagctaacc t
//
LOCUS       DAOQFT010000376         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_26222_length_2528_cov_6.581884, whole genome shotgun sequence.
ACCESSION   DAOQFT010000376 DAOQFT010000000
VERSION     DAOQFT010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26222_length_2528_cov_6.581884"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PLR93_09620"
     CDS             <1..1347
                     /locus_tag="PLR93_09620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNH27267.1"
                     /translation="KFMGWLDKPSERGSIIIKLGLMMYDAYTGSQRTVPRHTFYSRTE
                     SLTKWNKLNPEIVNTAVYYDGLISNPERLALEIMLDAETENSNARALNYVSLVSGSGK
                     NVILRDELTDATVEIQPKLVINAAGPWIDFANHSLGVTTKFIGGTKGSHLVLDHPELR
                     KAIGDNEFFFENKDGRIVLIFPLQDRVLIGTSDIKIDHPDDARCTDEEVDYFLELTAR
                     VFPNIKVTREHVVFAFSGVRPLASSMAKTTGQYSRDHHIEVLSGDWTNLSFPVYSLVG
                     GKWTSFRAFSEEVADKSLQFLGIKRNKGTQELPVGGGRNYPRTSEERTKYINGLAAWT
                     GLSHERLETLFERYGTRAESIAEFMKVATDAPLNSLPSYSKRELLFLIQHEKAEHLDD
                     LLLRRTMLAMLGKLTRDAVIECSMLMADALNWNLERRNAEAERTLRVLADRHRVRL"
     gene            1411..2376
                     /locus_tag="PLR93_09625"
     CDS             1411..2376
                     /locus_tag="PLR93_09625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH27268.1"
                     /translation="MSPRQKSKLQIDLVIPVYNEAEAIRQTYADIRKVVDALPYDFRF
                     IYVDDGSSDGTADTLRGISTDDPRVTLLQLSRNFGHQAALTAGMDASQGDVMISMDGD
                     GQHPPALIPKMVSLIEQGYDVVQGQRLEEERAASFKKVTSNFFYWLINRISGTQVLQG
                     AADFRALSRNALNGVRSMHEYHRFLRGMISWMGYNSVILPYHEPDRIAGKSKYSLGKM
                     VRLASDAIFSFSLAPLYIGLSAGFVFFILAGVQLIYVLSLWLTGNTQRVEPGWSSLMG
                     VLLIASGIIMILLGFIGVYVGYIFQEVKRRPIYLVKGRTPDARDE"
BASE COUNT          602 a          616 c          659 g          651 t
ORIGIN      
        1 aagtttatgg gctggctcga taaaccttct gaacgcggct cgatcattat caaactaggg
       61 ttgatgatgt acgatgccta tacaggcagt caacgcacag tgccgcgcca cacattctat
      121 tcccgcaccg aatcccttac caaatggaat aagttaaatc ctgagatcgt caacacggcg
      181 gtctattatg acggattgat ttctaaccca gaacgcctcg cacttgagat catgctggat
      241 gctgaaacag aaaattcaaa tgcccgcgct ttgaattacg ttagccttgt gagcggatcg
      301 ggcaagaacg tcatcctccg tgatgagttg accgatgcaa ccgttgaaat ccagccgaag
      361 ttggtcatta atgccgccgg tccatggatc gactttgcca accacagcct gggtgtgact
      421 acgaaattta ttggcggcac gaaaggttca caccttgttc tcgatcatcc cgaattgcga
      481 aaagcgatcg gcgataacga atttttcttt gaaaataaag atgggcgtat cgtgttgatc
      541 ttcccgttac aagaccgcgt tttgatcggc acctctgata tcaaaatcga tcatccagac
      601 gatgctcgtt gcaccgatga agaagtggac tactttcttg aactaaccgc gcgtgttttc
      661 ccgaatatca aggtgacacg tgaacatgtt gtttttgcat tttcgggcgt gcgtcctctg
      721 gcaagtagca tggcaaaaac cactgggcaa tacagccgtg accaccatat tgaagttctc
      781 agtggcgatt ggaccaacct aagcttcccg gtctattctc tggtgggtgg caagtggacc
      841 tccttccgtg ctttctctga ggaagtggca gataagtctt tgcagttcct cggtattaag
      901 cgcaataaag gcacacagga acttccagtt ggcggcgggc gcaattatcc gcgcacgagc
      961 gaagagcgca ccaaatacat caacggtctc gccgcgtgga cagggctttc gcatgaacgc
     1021 cttgaaaccc ttttcgaacg ttacggcacg cgcgccgaat ctattgctga gtttatgaaa
     1081 gtggcaactg atgcccctct taattcgttg ccgagttatt ctaaacgcga attgctcttc
     1141 ttgattcagc atgaaaaggc agagcatctt gatgatctac ttctgcgtcg caccatgctt
     1201 gccatgttgg gcaaactcac acgcgatgca gtgatcgaat gctccatgtt gatggcagat
     1261 gccctgaatt ggaacctcga acgtagaaat gctgaggcgg agagaacgct gcgtgtcttg
     1321 gcagaccggc accgggtccg gttatgaagc gatataaacg aacgccgccg ttatcggcgg
     1381 tgtttttgtc tgcgtggtag aatcttcctg atgtctccac gtcaaaaatc caaactccag
     1441 atcgaccttg tcattcctgt ttataacgaa gctgaagcca ttcggcaaac ctatgcggac
     1501 attcgcaaag tggtggatgc gctgccgtac gattttcgat tcatctatgt ggatgacggc
     1561 tcaagcgacg ggacagcgga tacgcttcga gggatctcaa ctgacgaccc aagagtgact
     1621 ctgctccagc tcagccgcaa ctttggtcac caagccgcgt tgaccgccgg tatggacgcc
     1681 tcgcaaggcg acgtgatgat ctcgatggat ggggatggac agcacccgcc tgctttgatt
     1741 cccaagatgg tcagtttgat cgaacagggc tacgatgtgg ttcaagggca gcgtctcgaa
     1801 gaagaacgtg cggcttcgtt caagaaagtc acttctaact ttttctattg gcttatcaac
     1861 cgcatcagcg gtacacaggt tttacaaggg gctgcagatt ttcgcgcctt gtcgcgtaat
     1921 gcattgaacg gcgttcgttc gatgcacgaa tatcatcgct tcttgcgcgg catgatttca
     1981 tggatggggt acaacagcgt catcctgccg tatcatgagc cagaccgtat tgctgggaaa
     2041 tcaaagtatt cacttggcaa aatggtgcgc ttggcttcag atgcgatctt ttcgttctcg
     2101 cttgcgccgc tgtatattgg tttaagtgct ggctttgtct tttttatttt agcgggcgta
     2161 cagttgattt acgtcctctc tttgtggctt accggcaata cgcaacgcgt ggaacccggc
     2221 tggagttcat tgatgggcgt gctgctgatt gccagcggca ttattatgat cttgctgggt
     2281 tttattggcg tgtatgttgg ctatatcttt caggaagtga agcgccgccc aatttatctt
     2341 gtgaagggga gaactccaga tgcccgagac gaataaacct aagatattca atatcacttt
     2401 gctgcggcat ggcgagtcag tggggaatgc tgaggcacgc tggcagggac aatcagattt
     2461 cccgcttacc gagcgcggac ggaagcaagc gcaagcctta gccgagcgtt ggaagcgcga
     2521 aaagatga
//
LOCUS       DAOQFT010000377         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_26272_length_2525_cov_5.957490, whole genome shotgun sequence.
ACCESSION   DAOQFT010000377 DAOQFT010000000
VERSION     DAOQFT010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26272_length_2525_cov_5.957490"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLR93_09630"
     CDS             <1..1002
                     /locus_tag="PLR93_09630"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNH27269.1"
                     /translation="GRPRPYGILIMKNQAADIHRNSIVWDTHACFPLNPKSDLSELKR
                     YKDSGVTFVSLNIGMDMDSFENVMRVLARFRAHIAAYPHELILAQTVEDIQRAKREHK
                     LAIAFDLEGSNPLCDDLNLISFYYDLGVRQMLLAYNRDNAASGGCMEGQIGLTNFGRQ
                     VVQEMNRVGMVVDVTHMGYRATMDTFETSSAPVIFSHSNPASLHAHARNISDEQIKAC
                     AKTGGVIAINGIGDFLGGKTSELFVQHLEYVLNLVGPEHVGIGLDYVIDKQELIDYIQ
                     GNPDVFPADRFEDFLSFVEPEQLPEISEILYKHGHDEDVIRKVLGGNFLRVAEQTWK"
     gene            1030..2181
                     /locus_tag="PLR93_09635"
     CDS             1030..2181
                     /locus_tag="PLR93_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNH27270.1"
                     /translation="MSTILLTGGRAPVTLDLARAFHRAGHVVYVAESLRGHLTEPSAA
                     IKAHFEVPAPRQEPEAFLAALRSIIEAYQIELLIPTCEEVFHIAKGLNTLPCRVFTEP
                     LARLDGYHNKWKFVVKAGECDLRVPESMLVTRQDDLLYAYAHWRGLVLKPVYSRFAAR
                     TLILPPLKDAMATLTFDSLTNQEPWIAQEYIEGEQFCSYSVCQNGRILAHAVYPTAFT
                     AGQGATIAFQSVQHDAIYEWVRTFVEHYKVTGQMAFDFVQTADGNVVALECNPRATSG
                     AHLLTSNPRFVESFFNSELECITPYEGTSRMLGTAMLTYGLPTSLFNGRFGAWLKTFS
                     HSDDVLLDFRDPLPFLLQWRSILSYVLLGRKQGISALEASTFDIEWNGE"
     gene            2318..>2525
                     /locus_tag="PLR93_09640"
     CDS             2318..>2525
                     /locus_tag="PLR93_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27271.1"
                     /translation="MNIESKILYIKQSLDKLKALDKGYRVFGADSHKYLLNEVAKEEK
                     IKVFENKYGIQLPEEYRRFLIEVGN"
BASE COUNT          629 a          577 c          678 g          641 t
ORIGIN      
        1 gggagacccc gcccctacgg tatcttaatt atgaaaaacc aagccgcaga cattcaccgc
       61 aattccatcg tatgggatac gcatgcctgt ttcccgttga atcccaagtc agatctgagc
      121 gagttgaaac gctataaaga cagcggcgtg accttcgttt cgctcaacat cggcatggac
      181 atggactcgt tcgaaaatgt gatgcgggtg ctggcgcgct tccgggcgca tatcgcggct
      241 tatcctcatg agctgatcct tgcacagacg gtggaagata ttcaacgcgc caagcgagaa
      301 cataaattag ccattgcctt cgatctggaa ggctcgaacc cactctgcga tgacttgaac
      361 ctgatctcgt tctattacga ccttggcgta cgccagatgt tattggcata taacagagac
      421 aatgccgctt cgggcggttg catggagggg cagatcgggc tgacgaattt cggcaggcag
      481 gtggtgcaag aaatgaatcg cgtgggcatg gtggtggatg tgacgcacat gggctaccgc
      541 gccacgatgg atacctttga gacctctagt gcgccggtca tcttttcaca ttcgaaccca
      601 gccagcttac acgcccatgc ccgaaatatt tcagatgagc agatcaaagc atgtgccaag
      661 accggtggcg tgatcgccat caacggcatt ggcgattttt taggcggcaa aaccagcgag
      721 ctttttgtac aacaccttga gtatgttttg aatttggtcg gacccgaaca tgtgggaatt
      781 gggttggatt atgtcatcga caaacaggaa cttatcgact acattcaggg aaatccggat
      841 gtcttcccag ccgaccgctt tgaagatttc ctttcgttcg tagaaccaga acaattgcca
      901 gagatcagcg agattttata caagcacggg catgatgaag atgtgatccg taaggtgttg
      961 ggtgggaact tcttgcgggt ggcagaacaa acctggaagt aaaaaatccc ttattggcag
     1021 gctgacctga tgtcaaccat tcttctcacc ggcgggcgag cacccgtcac cttagatctg
     1081 gcgcgtgcgt ttcaccgagc agggcatgta gtgtatgttg ccgagtcttt acgcgggcat
     1141 ctcaccgagc cttcggcggc gatcaaggct cattttgagg tgccagctcc acgtcaggaa
     1201 cctgaggcgt ttttagcggc gttgcgaagc atcatcgaag cgtatcagat cgagttgctg
     1261 attcccacct gtgaggaagt tttccatatt gcaaaagggt tgaatacttt accctgccgc
     1321 gtgtttaccg agccgctggc aaggttggat ggatatcaca acaagtggaa gtttgtggtg
     1381 aaggcgggag aatgcgattt gcgcgtgcca gagtcgatgt tggtgacgag acaagatgat
     1441 ttgctctacg cctacgctca ctggcgcgga ctggtgctga agccggtcta ttcccgcttc
     1501 gctgcgcgga ccttgatctt gcccccgtta aaagatgcca tggctacgct gacgtttgac
     1561 tctctcacga accaagagcc gtggatcgca caagaatata ttgagggaga gcaattttgt
     1621 agttattcgg tttgtcagaa tggacgcatt ctagcgcatg cggtctaccc tacggctttc
     1681 acagccgggc agggagcaac gattgcgttt caatcggtgc agcatgatgc catctatgaa
     1741 tgggtgcgga cctttgtgga gcattacaaa gtgaccgggc aaatggcgtt cgattttgtg
     1801 caaacagcag atggcaacgt ggtggcgttg gaatgtaacc cacgtgccac aagcggagcg
     1861 catttgctga cctcgaaccc gcgctttgta gagtctttct ttaacagtga actggagtgc
     1921 attacaccct acgaagggac ttcgcgtatg ttgggcacag ccatgttgac ctatgggctg
     1981 cccacctcgc tatttaacgg tcgctttggg gcgtggctaa aaaccttctc tcacagcgac
     2041 gatgtgctct tagactttcg cgacccattg cccttccttc tgcaatggcg cagcatcctt
     2101 tcatatgtgt tgctgggtcg caagcaaggc atctcagcat tagaggcatc cactttcgat
     2161 attgaatgga atggagagta gggtattagg ttggaacgtc taattaaaaa taaaattggc
     2221 aattaaggta tgtttcctta agtctttttc tgactggact tggctaggtc aacgtatgaa
     2281 gcttagggat ggaaagtcca ttcgggtggt ttagcttatg aatatagaat ctaaaatact
     2341 gtatataaaa caatctctag ataaactaaa ggctttggac aaaggctatc gtgtctttgg
     2401 cgcggacagt cataaatatt tactgaacga ggtggcaaag gaagaaaaga taaaagtatt
     2461 tgaaaataaa tatggtattc aactcccaga agagtatcga agatttctca ttgaagttgg
     2521 gaatg
//
LOCUS       DAOQFT010000378         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_26346_length_2520_cov_9.157404, whole genome shotgun sequence.
ACCESSION   DAOQFT010000378 DAOQFT010000000
VERSION     DAOQFT010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26346_length_2520_cov_9.157404"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLR93_09645"
     CDS             <1..377
                     /locus_tag="PLR93_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27272.1"
                     /translation="GAVLLAAGFIEEVVKTVGIVVLAERGVIKSVREILWLSFLSALG
                     FLIGEKLLVFTSVSVVAQAEVSTALFQAGVFLLIPLAAHFIFVSVVNLLRFKLRFPYW
                     LALAVGTILHVIYDATVLRGGI"
     gene            374..1408
                     /locus_tag="PLR93_09650"
     CDS             374..1408
                     /locus_tag="PLR93_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH27273.1"
                     /translation="MIYPFLAIVKKELSSVLRDRTIVISILIQFFIASFSSGLLLGML
                     SLYDADTIMKFSGPNMRIGVIESADTALADRIEERGLNVIRFETLAEAQTAFYDNEIL
                     AIVDTPTDVNGLTEVKLYLPDSDTVSSLIRMVIQEPLKQYENDLRQANGVEIYYTDLK
                     GKPATSFEFVYSVLIPMLMFFPAFVAGSMSIDAITEEVEGKTLQTLLSTPLTVSTMIG
                     AKIVSAVILAVLQCAAWLALIQINGTVIQNRAWILLLALVIAGITSTSAALSATFLQD
                     RERSQFIYSLLLLAGVGISTLLNISPITTLSRLAIGDAYTNGWDVLVFALAFAALFGF
                     LLRASRRFNA"
     gene            1499..1918
                     /locus_tag="PLR93_09655"
     CDS             1499..1918
                     /locus_tag="PLR93_09655"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNH27274.1"
                     /translation="MTNSLSAIWHQPTFANLLESRTETGAIVAFGVVHVGLNLFGVSL
                     WQCPILAATGVPCPGCGLTRATMQLLHGDLIASLQTHAFAPIFMIALILMGLVFILPT
                     NLSQNIISSVRRLEVRNGLTAWVLCSLMLYWAVRLMA"
     gene            1945..2199
                     /locus_tag="PLR93_09660"
     CDS             1945..2199
                     /locus_tag="PLR93_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27275.1"
                     /translation="MEVSANMGLGGVIAFVAGIWVLIKLFQKEGLMKGILGFICMLYT
                     FIWGLQNMKNEDLKLKTPMYLWIGGIVLGIIINFVAGASG"
     gene            2333..>2520
                     /locus_tag="PLR93_09665"
     CDS             2333..>2520
                     /locus_tag="PLR93_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNH27276.1"
                     /translation="MTDKKAVFPEGATILGAYSPGLISGGFLFISGQVGKAPDGTLGA
                     TIEEQAKQTIENIGAILRA"
BASE COUNT          582 a          606 c          588 g          744 t
ORIGIN      
        1 ttggtgctgt gttacttgcg gctggtttca ttgaagaagt cgttaaaacg gttgggatcg
       61 ttgtattggc agaacgcggt gtgattaagt ctgtgcgcga aattttatgg ctctcgtttc
      121 tctctgcatt gggatttttg atcggcgaaa aactgctggt ctttacatcc gtgagtgtgg
      181 ttgcacaagc tgaggtttca acagcgttgt ttcaagccgg agttttcttg cttattccac
      241 ttgccgctca tttcattttt gtctcggtcg taaatttatt gaggttcaaa ttgcgttttc
      301 cgtattggtt ggcgttggca gtaggcacca ttctgcatgt gatctatgat gcgaccgtgt
      361 tacgaggtgg aatatgatct atccattctt ggcgatcgta aaaaaagaac taagttccgt
      421 cctgcgcgac cggaccattg tcatttcgat cttgatccaa ttctttatcg cttcgttttc
      481 ctccggtttg ttattgggaa tgctttccct ttacgatgcg gatacgatca tgaaattcag
      541 cggacccaat atgcgaatcg gcgttattga aagcgcagat accgcccttg cagaccgcat
      601 cgaagaacgc ggacttaacg tgattcgttt tgaaaccttg gctgaggcgc agactgcctt
      661 ttatgataac gaaatccttg ccatcgtgga tacaccaacc gatgtaaatg gtctgaccga
      721 ggtcaaactg tatttaccag actccgatac ggtcagttcg ttgatccgta tggtgattca
      781 agaaccgctt aagcaatacg aaaatgactt acgtcaggcg aacggtgttg aaatttacta
      841 cactgactta aaaggaaaac cagcgacatc gtttgagttc gtctattccg ttctgattcc
      901 tatgctgatg ttcttccctg cgtttgtagc aggtagtatg tctattgatg ccatcacgga
      961 agaagtagaa ggcaaaacgc tgcaaacctt actttctacg ccactcaccg tcagcacaat
     1021 gatcggtgcc aagattgtct cggcggtgat tctggctgtc ttgcagtgtg ccgcatggct
     1081 ggctctcatt caaatcaacg ggactgtcat tcaaaaccgc gcctggattt tgctactggc
     1141 gctggtcatt gcaggaatca catccacctc tgcggcattg agcgctacct ttctccaaga
     1201 tcgcgaacgt tcacaattca tttactcctt gctattgttg gctggtgttg gcattagtac
     1261 cttgcttaac atctcaccca tcaccacgct ttcaaggctc gccatcggtg atgcctacac
     1321 caatggttgg gatgtgctgg tctttgccct agcatttgcc gcccttttcg gatttctctt
     1381 gcgcgcatca cgccgcttca acgcctaaca tcgcctcaga ctccgctcta gcctgttact
     1441 tcacccaagc tcttaattcc cctgctaact tttaaaaagc aagagggttt tccttaacat
     1501 gaccaactct ttatccgcca tctggcatca acctacattt gcaaacttgc ttgaaagccg
     1561 cacagagact ggcgccatcg tcgcctttgg agtggtgcat gtagggctga atctgtttgg
     1621 ggtttcactc tggcagtgtc ctatcctagc tgctactgga gtcccatgcc ccggctgcgg
     1681 tttgacccgt gccaccatgc aattgctcca cggagatttg attgcctccc ttcaaaccca
     1741 tgcctttgcg ccaattttca tgatcgccct gattttgatg gggctagtat ttatattgcc
     1801 aaccaacctg tcgcaaaata ttatcagttc cgttaggcgg ctagaagtgc gaaacgggct
     1861 tactgcatgg gtgttatgtt cgctaatgct atattgggca gtacgcctaa tggcataaaa
     1921 ccaattcatc taggagagtg tgaaatggaa gtttctgcaa atatggggct tggtggtgtg
     1981 attgccttcg tcgcaggcat ctgggttctg atcaagttgt tccaaaaaga aggtttgatg
     2041 aaaggcatct tgggctttat ctgcatgctt tataccttca tctggggtct tcagaacatg
     2101 aagaacgaag acctcaaact caagaccccc atgtatcttt ggatcggcgg tatcgttctg
     2161 ggaattatca ttaactttgt tgccggtgcc agcggctaat ttgcccaatt aaaatagaac
     2221 agcctccttt ttgggaggct gttctatttt aagaagttca acccattggg ggatttttca
     2281 gagaacccat tcaggtatac tgagccaact tagcgttaaa ggagacatta acatgacaga
     2341 caaaaaagca gtattccctg aaggtgcaac cattttaggt gcctattcgc ccgggctcat
     2401 tagcggcgga tttttattca tctcgggtca ggtcggcaaa gctcccgatg ggaccttagg
     2461 cgcaaccatc gaagaacaag ccaaacagac tattgagaac atcggcgcca tcctccgcgc
//
LOCUS       DAOQFT010000379         2519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_26369_length_2519_cov_4.942776, whole genome shotgun sequence.
ACCESSION   DAOQFT010000379 DAOQFT010000000
VERSION     DAOQFT010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26369_length_2519_cov_4.942776"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLR93_09670"
     CDS             <1..221
                     /locus_tag="PLR93_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27277.1"
                     /translation="VAWFRALNKYPSLQLWQEDGSHPSEQGTYLAASVFYVTLFKESP
                     VGLNYHANLTEEVASQLQSVAESILTNP"
     gene            245..613
                     /locus_tag="PLR93_09675"
     CDS             245..613
                     /locus_tag="PLR93_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27278.1"
                     /translation="MPTTSEFPIVFEHLKSILVPYAKKMTVKNDISGLYYLDAGYSEK
                     WKRDLFFASVQIKKSYVSFYLMPVYMYPDLLKNISPELKKHMQGKSCFNFKKIEKSLF
                     EELSQLTKQGHERMMKEEFS"
     gene            complement(653..>2519)
                     /locus_tag="PLR93_09680"
     CDS             complement(653..>2519)
                     /locus_tag="PLR93_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNH27279.1"
                     /translation="YTLTVAGFVNDLSRLPAEISLAGIGYITPETANELELEPEYNQL
                     LIRYADGSTRTEVEKLTTTVTEAIEEDEYIVFNANVPVPDTYILGDNMTSVLFILNSI
                     GFLTLILSAFLVTSVMSAMMSQQIPQIGILKSLGAHVGQTMSIYFREVLLFGIFALIL
                     AIPMGLVGAYFLADGLASSMNFDVPNFYLPTQTLILQAISALLAPLLAALIPIISGSR
                     ITIREALSNYTPDSASNALSLGEIPQIANLSIRNTFRRKSRLALTFAALLLAGSMFIS
                     IIGIREEMRQASIEMQKEANYDLDVDLARPYSQSTLEETALRVKGVEEVETWAVDTGR
                     LIFNKDRLSGSIILYGIPQGTTMTHPAVINGTWLSKPKSIFVNNDFLLLSPDLKVGSV
                     ITLNIGGKEEEWTIVGSTGRGFIPIAYVYYDDLTNVTGLDGFASRLVVRTDKSDSAFQ
                     TSAQSDVLSHLAAEDIDVVNSSTTTERNETSLEQMDTLIILLMAMVILIAIVGGLGLA
                     ITMSLNVMERTREIGILRSLGAQNGVVRRVVMIEGLVIGVISWAISIPCSVPLSIFLG
                     NSLGLSLLARPLDYIFSVPAMLIWLALTVVIAIIASILPAQSAAHLTIRDALVYE"
BASE COUNT          684 a          564 c          666 g          605 t
ORIGIN      
        1 gagtggcatg gtttcgggcg ctcaataaat atcccagcct acagctttgg caggaagatg
       61 gtagtcaccc ttctgagcaa ggcacctatc ttgctgctag tgttttctat gtcacccttt
      121 tcaaagaaag ccccgtaggt ttgaactacc atgcaaacct tacggaagaa gtcgctagtc
      181 aattgcaatc tgtggctgaa agtattctga ccaacccata aagtaacaat aaaggagaga
      241 actcatgcct acaacatcag aattcccgat tgtatttgaa cacctcaaat ccattctcgt
      301 accttatgcc aaaaagatga ctgttaagaa tgatataagt ggactgtatt atttagacgc
      361 gggctacagc gaaaagtgga agcgcgacct tttcttcgcc tcggtacaga tcaagaagag
      421 ttatgtctcg ttctatctca tgccggttta catgtacccc gatttgttga aaaacatctc
      481 gccagagctc aaaaaacaca tgcagggaaa atcctgtttc aatttcaaga aaatcgaaaa
      541 atccctgttc gaagagttaa gtcaactcac caaacaggga catgaacgca tgatgaaaga
      601 agaattttcg taattgtttt agggacgata tgcttgttgc tgtccctgaa ctttattcat
      661 acaccagggc gtctcgaatt gtaaggtggg cggcgctttg cgcaggcagg atgctggcaa
      721 tgatggcgat caccacggtt agggcgagcc agatcagcat ggcagggacg gagaagatgt
      781 aatccaatgg gcgggcaagc agcgaaagcc cgagggaatt gcccaaaaat atagagaggg
      841 gaactgagca gggaatggag atcgcccaac tgatcacacc gatcaccaag ccttcgatca
      901 tcacgacccg gcgaacgact ccattttgag cgcccagtga acgcaatata ccgatctcgc
      961 gggtgcgttc catcacattg agactcatgg tgatggcaag ccccagtccg ccgacgatgg
     1021 cgatcaggat caccattgcc atcagaagga tgatcagggt atccatctgt tcgagtgagg
     1081 tttcatttcg ttcggtggtg gtggatgaat tgacaacatc tatatcttca gcggctaggt
     1141 gggagagaac gtcagattga gccgaggttt gaaaggctga atcagattta tcggtccgca
     1201 ctacgaggcg gctggcgaag ccatccagcc cggtaacgtt ggtcaagtca tcgtagtaca
     1261 cataagcgat ggggataaat ccgcgcccgg tggagccaac gatggtccac tcttcttctt
     1321 tgccgccaat gttcagcgta atgacagagc caacttttag atcgggggag agcaaaagaa
     1381 aatcgttgtt gacgaagatg cttttgggtt tggataacca tgtgccgttg atgaccgcgg
     1441 ggtgggtcat ggttgtgcct tgtggaatgc catacaaaat gatgctgccg ctcaggcggt
     1501 ctttattgaa aatgagccga cctgtatcca ctgcccaggt ttcaacttct tctacacctt
     1561 ttacacgcaa ggcagtttct tccaacgtgc tttgagaata tgggcgcgcc aagtctacat
     1621 cgagatcgta attcgcttct ttttgcattt caatactggc ttgccgcatt tcttcgcgaa
     1681 tgccgataat ggaaatgaac atggagccag ccaggagcag tgcggcaaag gttaatgcaa
     1741 ggcggctttt gcggcggaag gtattacgaa tcgaaaggtt tgcgatctgc ggaatctctc
     1801 caaggcttaa tgcattggag gcactatcgg gagtgtagtt gctgagcgct tcacggatcg
     1861 tgatgcgcga accagaaatg atagggatga gcgccgccaa taatggcgca agcaatgcac
     1921 tgatggcttg caagatcagg gtttgagtgg gtaggtagaa attcggcaca tcgaagttca
     1981 tgctcgaagc cagaccatct gcaaggaaat aagcgccgac caagcccatt ggaatagcga
     2041 ggatcaaggc gaagatgccg aacagcagca cttctcgaaa atagatggac atggtctgac
     2101 cgacatgcgc gcctaaagat tttagaatac cgatctgagg gatctgctgt gacatcatgg
     2161 cagacatcac actggtgacg aggaacgcgc tcaggatcag tgtgaggaag ccaatcgaat
     2221 tcaaaatgaa aagaaccgat gtcatgttat cgccgaggat gtaagtatcg ggcacaggca
     2281 cattcgcgtt gaaaacgatg tactcatcct cttcgatggc ttcggtcact gtggttgtca
     2341 gtttttcaac ttcggtgcgc gtagagccat ctgcatatcg gatgagcagc tgattgtatt
     2401 ccggctcgag ttcaagttcg ttggctgttt ctggggtgat gtaaccaatc cctgctaggc
     2461 tgatttcagc aggtaaacga gaaaggtcat ttacaaaacc ggcaacggtc agggtgtat
//
LOCUS       DAOQFT010000380         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_26741_length_2496_cov_6.226956, whole genome shotgun sequence.
ACCESSION   DAOQFT010000380 DAOQFT010000000
VERSION     DAOQFT010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26741_length_2496_cov_6.226956"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..1515
                     /locus_tag="PLR93_09685"
     CDS             412..1515
                     /locus_tag="PLR93_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNH27280.1"
                     /translation="MFAGLDASFYELEEQYYLMLRSSIVPTLGCINSFVESGVLRELR
                     KISAELVVRHTLNGKAERPFWIDQEIPKIIEKVRKHCGFVNDIEVLISVEGRIHARAI
                     SNRKIVLACGFREFLRHCNLVLWTLLDLMQPSQLHDGFTSFILENTGDLARGILPYFL
                     AAFDLIPVANIPFVATSRVGILFTANQTARIQATYLLAHEYAHIALGHFNIDRRKLDR
                     EEKERLEIEADRFALNIVVELANDSEIDKPGDVWLALRWLHEYRIIEEVIAKVSAGAF
                     DSLDSLASTRRKNALNEMRNLTYFDSCDKADLAYASFGLVLLFALKSTIVSGGDKLIS
                     NILEQIAKVHDGPASSTWWKSMQIVLPDLYLAF"
     gene            1539..1904
                     /locus_tag="PLR93_09690"
     CDS             1539..1904
                     /locus_tag="PLR93_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27281.1"
                     /translation="MAISIVTSIQVEIFGESVSSLLDDTGSRINIENGLACLEVISKE
                     EGFAFGDTSVWDMVLTFATNVSAGVVANAVFSALGHQVRRIVINGRRVRPTREEVEKA
                     LEVIQLAMQSASNDKKDDL"
     gene            complement(2008..>2496)
                     /locus_tag="PLR93_09695"
     CDS             complement(2008..>2496)
                     /locus_tag="PLR93_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27282.1"
                     /translation="AQTDPESFAYLEPSADGFRNYYGANNRVSPAEMLVDKASSLDLT
                     VPEMTVLIGGLRALDANSGQSANGVFTKRPGTLSNDFFVNLLDMSTVWKKSDQAGGLY
                     EGIDRATGARKWTATPADLIFGSNSELRAVAEAYAADDAKEQFARDFVAAWTKVMNLD
                     RF"
BASE COUNT          611 a          510 c          699 g          676 t
ORIGIN      
        1 tgaaaagcgc catcgtcgcc gaccccgatc acggttccga cagcaccttg ttcgaggctt
       61 tcggcggcat ccgcgtcagt gagcggcgca gcggcttgac acgcaagcgc aatgattcga
      121 cggatgacaa gacggggacg tgataagggg gagtggcaga tgcagcaggg tgtgcagctt
      181 tgctgcgcac cttgcagcgt gtcatgaatg ggtaacgtaa atggtgcgca gcaagctgca
      241 cacccacaag gttagaaaaa ctatcttggt gcgataggtt aggggtgatt agaaaatgaa
      301 tataatggaa tttggcggtg agttgttaat tgacgcagac ggtttttttt tccgtatttt
      361 tctcaggaca gtgtcggaaa agaaaattca aagaatgaaa atttaatgca tatgtttgct
      421 gggcttgacg cttcttttta cgagcttgaa gagcaatatt atctgatgct tcgctcttca
      481 atagttccga cgttagggtg tattaattct tttgtggaga gtggggtgtt gcgtgaatta
      541 agaaaaattt ctgcagaatt ggtggttagg cacactttaa atggaaaagc agaaagacca
      601 ttttggattg atcaagagat tcctaagata attgaaaaag tgcgtaagca ttgtgggttc
      661 gtaaatgaca ttgaagtatt gatctcggtg gaagggcgta tacatgctcg tgctatatca
      721 aatagaaaga ttgttttggc atgtgggttt cgtgagtttt tgcgtcattg taatttggtg
      781 ttgtggaccc tgttagatct gatgcaacca tctcaattgc atgacggttt tactagtttt
      841 attcttgaaa atacgggaga tctggcgcga ggaattttgc catacttcct cgctgcgttt
      901 gacttgattc ccgttgctaa cattccattc gttgcaacat ctagggtcgg aatactcttc
      961 actgctaatc aaacggcaag aattcaagcg acctacctcc ttgctcacga atacgcacat
     1021 attgcactag gccatttcaa tattgacaga agaaaattag atcgggaaga aaaagaaaga
     1081 ttggaaattg aggccgacag attcgcgcta aatattgttg ttgaactcgc aaatgattcc
     1141 gagattgaca agccaggtga tgtttggttg gcgttacggt ggttgcacga gtacagaata
     1201 atcgaagagg ttattgctaa ggtaagtgca ggcgcgtttg actccttgga ttcgcttgcg
     1261 tctaccagaa gaaagaacgc gttaaatgag atgcgaaatt tgacttattt cgatagttgt
     1321 gataaagctg atttagctta tgcatctttt ggattggtgc tcttgtttgc gctaaaatcc
     1381 actattgttt ctggtggaga taaactgata tcgaatattt tggaacagat tgcgaaagtg
     1441 catgacggtc ctgctagctc cacatggtgg aaaagcatgc aaattgttct tcccgatctt
     1501 tatcttgcat tttgatgcca aattggctgg agtaggatat ggctatatca attgtgactt
     1561 cgattcaagt tgaaattttt ggtgaatcgg tgtctagtct cttggatgat actggttcga
     1621 gaataaacat cgaaaatgga cttgcttgcc ttgaagtaat ttctaaggag gaagggtttg
     1681 catttggtga tacatccgta tgggatatgg tgttgacatt tgcgacaaac gtttcggcgg
     1741 gggtggttgc aaatgcagtt ttttcagcgc ttgggcatca ggtgcgccgc attgtcatca
     1801 atgggcgcag agtccgccca acacgcgagg aggtcgagaa agcgctagaa gtaatccagt
     1861 tagcgatgca gtctgcttca aacgataaaa aggatgattt ataagtttag caagcgtagg
     1921 gcggattagg tgcgcatggc acgacatcaa atcaccgcga tgctttcccg cgcgccgtaa
     1981 tccgccttcc gcgtttgccg caagccctca gaaccgatcg aggttcatga ccttggtcca
     2041 ggcggccacg aagtcgcggg cgaattgctc ctttgcatcg tcggcggcgt aggcttcggc
     2101 gacggcgcgc agttcggagt tggagccgaa gatcaggtct gccggcgtgg cggtccattt
     2161 ccgggcgccg gtcgcgcggt cgatgccttc atacaagccc ccggcctgat ccgatttctt
     2221 ccagaccgtc gacatgtcga gcagattgac gaagaagtca ttcgacagcg tgccggggcg
     2281 cttcgtgaac acgccgttgg cggactgccc gctgttggcg tcgagggcgc gcaggccgcc
     2341 gatgagcacg gtcatttccg gcacggtcag gtccagtgaa ctcgccttgt cgacgagcat
     2401 ctccgccggt gacacgcggt tgttcgcgcc gtaatagttg cggaaaccgt cggcggaagg
     2461 ttccagatag gcgaaggatt ccgggtcggt ctgggc
//
LOCUS       DAOQFT010000381         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27018_length_2480_cov_6.054845, whole genome shotgun sequence.
ACCESSION   DAOQFT010000381 DAOQFT010000000
VERSION     DAOQFT010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27018_length_2480_cov_6.054845"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1068)
                     /locus_tag="PLR93_09700"
     CDS             complement(31..1068)
                     /locus_tag="PLR93_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27283.1"
                     /translation="MDTPAIRFESVSRHFGDVKAVDQVEMEIRDGEFFSMLGPSGSGK
                     TTCLRMIAGFDRPTSGHIYLYGQDVSNLPPYERDVNTVFQDYALFPHMSVGDNIAYGL
                     MIKKVEKVERMKRVEEMLDLVQLSGFAGRKPSQLSGGQRQRVALARALINHPKVLLLD
                     EPLGALDLKLRQQMQVELKSIQKRVGITFIFVTHDQEEALTMSDRIAVFSQGKIEQIG
                     TPSEVYEHPASQFVAGFVGTSNLISGDVAERITGHAQTFSIRPEKIHIGSQQEYISSD
                     MYAVDGTIRDVIYLGLYTRYLVEITQGIDLVVISQNLKTTSADVFSSKGSAVRLSWQQ
                     DHISILGGKGA"
     gene            complement(1156..2337)
                     /locus_tag="PLR93_09705"
     CDS             complement(1156..2337)
                     /locus_tag="PLR93_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH27284.1"
                     /translation="MKKQLFNMLVVFALASLVLAACGGSGGTASEMKTEVGDGEGEVS
                     IISWAGYIERGETDPNYDWVTEFEAQTGCKVTNKTAATSDEMVTLMNEGGFDLVTASG
                     DASNRLIAGGKVQPINIDLIPSWSTVDDRLKDAPWHTVGGVHYGVPYQWGPNVLMYNT
                     EVFTEAPTSWDVTFEKMDLPDGKSNEGRVQAYDGPIYIADAALYLKYHKPELGIENPY
                     DLTEEQFNAVVELLTAQRSLINRYWHDAYVQMDDFKNEGVVASGSWPFQVNLLAFEGA
                     PIASTFPVEGATGWADTTMMHVDAPHPNCAYKWLEWSLNPKLQGDLAAWFGSVPSVPA
                     ACTGNELLTDAGCSANGFDQFDNIWFWRTPTAECSTGAGNCVPYSKWVEAYVGIISSG
                     Q"
BASE COUNT          659 a          607 c          605 g          609 t
ORIGIN      
        1 ggatgccggt acaaataggt ggagatgcgg ttaagcacct ttgcctccca aaatactaat
       61 gtggtcttgt tgccatgaaa gccgcacggc actgccctta gacgagaaga cgtcggcaga
      121 ggttgttttc aaattttgtg aaatgaccac aaggtcgatg ccttgcgtga tttcaaccaa
      181 atagcgagtg tacaagccaa gataaatcac atcgcgaatg gtgccatcca cggcgtacat
      241 atctgagcta atgtattcct gttgtgaacc aatatgaatc ttttcaggac ggatggaaaa
      301 agtctgcgcg tgacctgtga tgcgttcggc gacgtctccg ctaatcaaat tggatgtacc
      361 cacaaacccc gccacaaact gcgacgcggg atgttcatac acttcagacg gggtaccaat
      421 ctgttcaatt ttcccctgac taaaaaccgc aatgcgatca ctcatggtga gcgcctcttc
      481 ttgatcgtgc gttacgaaga tgaaggtaat acccacccgc ttttgaatgg acttaagctc
      541 cacctgcatt tgctggcgta atttcaagtc taatgcgccg aggggttcat cgagtagcaa
      601 aactttgggg tgattgatca aggcacgggc aagtgcgacg cgttggcgct gtccgcctga
      661 gagttgggaa ggtttgcgtc cggcaaagcc agagagttgg acgagatcca gcatttcttc
      721 cactcgtttc atacgctcaa ctttttctac ctttttgatc atgagaccat aggcaatatt
      781 atcgcccaca gacatatgag ggaaaagagc ataatcttga aaaacggtat taacatcgcg
      841 ttcatagggc ggaagattgg aaacatcttg accgtataaa taaatatgcc cagaggtagg
      901 tcggtcaaac cccgcaatca ttcgcagaca ggtggttttg cctgaacctg aaggtcctaa
      961 catggaaaaa aattcgccat cgcgaatttc catttccact tgatctacgg cttttacatc
     1021 tccaaaatgg cggctaacag actcaaaacg aatcgcaggt gtatccataa aactccgtaa
     1081 aaaagcaata ttcaggcgga gccattgccc cgcctgaata ttttacatca cgttactaga
     1141 attctggcag caggattact gtccgctgct gatgatgcct acgtaggctt caacccattt
     1201 gctgtaaggt acacaattgc cagcaccggt ggagcattcg gcggtcggcg tgcgccagaa
     1261 ccaaatgtta tcgaactgat cgaaaccatt ggctgaacag ccagcatcgg tgagcagttc
     1321 gttgcccgta caagcggcag gcacagaagg cacggaacca aaccaagccg caaggtcgcc
     1381 ctgcagtttg gggttcaagg accattcaag ccatttatat gcgcagttgg ggtgaggagc
     1441 atccacgtgc atcatggtgg tgtctgccca gccggtggcg ccttcaacag ggaaggtgga
     1501 ggcaatcggt gcaccttcaa aggcgagcag gttcacctgg aaaggccagg aaccagaagc
     1561 cacaacgcct tcgttcttga aatcgtccat ctgaacatag gcatcgtgcc aatagcggtt
     1621 gatcaaagaa cgctgagcgg tgagcaattc aacgacagca ttaaattgtt cttcggtgag
     1681 atcgtaggga ttttcaatac cgagttctgg cttgtgatac ttgagataca aagcagcgtc
     1741 ggcaatgtag ataggaccgt cataggcttg aacgcgtcct tcgttggact tgccatcagg
     1801 aaggtccatc ttttcgaagg ttacatccca tgaggttggg gcttcagtga aaacttctgt
     1861 gttgtacatc aacacatttg gaccccactg ataagggaca ccatagtgaa cgccacccac
     1921 ggtatgccag ggagcatcct tgaggcgatc gtcaactgta gaccaagagg ggatcaaatc
     1981 aatgttgatg ggctgaacct tgccacctgc aatgaggcgg ttggaggcat cgccagaagc
     2041 ggttacgagg tcaaatccgc cttcgttcat caaggtaacc atttcgtcag aggttgcagc
     2101 agttttgttg gttactttac aacctgtttg ggcttcgaat tcagtcaccc agtcgtagtt
     2161 cgggtcggtt tcgccgcgtt caatgtaacc agcccatgaa atgatggaaa cttccccttc
     2221 accatcgcca acttctgtct tcatttcaga agccgttcca ccgctgccac cacaggcagc
     2281 aagaaccagg cttgctaaag caaaaacaac caacatgtta aacagttgct tcttcatatt
     2341 tctctctcct attctctttt tgattagttg aaacatcagg tcactttata gaaatggctt
     2401 gatctcttct gcaccgacct ccttttaggt cattaggaaa gaattcccca catccaatga
     2461 cagggatacg gggattaggc
//
LOCUS       DAOQFT010000382         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27158_length_2473_cov_4.836228, whole genome shotgun sequence.
ACCESSION   DAOQFT010000382 DAOQFT010000000
VERSION     DAOQFT010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27158_length_2473_cov_4.836228"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="PLR93_09710"
     CDS             <1..108
                     /locus_tag="PLR93_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNH27285.1"
                     /translation="DLPGVRYHIVRGTLDTTGVANRKQGRSKYGAKRPK"
     gene            129..596
                     /gene="rpsG"
                     /locus_tag="PLR93_09715"
     CDS             129..596
                     /gene="rpsG"
                     /locus_tag="PLR93_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNH27286.1"
                     /translation="MRRAKPERREILPDIRFNSINIQTMIQHVLKNGKKSVATGLIYD
                     AMDLIKERTEKNPMDVFDIALKNVGPVMEVRPRRVGGATYQVPMEVSADRRTTLAIRW
                     ILGAARDRSGKSFADKLASELIDASNETGAAIRKRDEAHKMAEANRAFSHYRI"
     gene            621..>2473
                     /gene="fusA"
                     /locus_tag="PLR93_09720"
     CDS             621..>2473
                     /gene="fusA"
                     /locus_tag="PLR93_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNH27287.1"
                     /translation="MARVHPLEKYRNIGIIAHIDAGKTTTTERIMFYTGLTHRIGSVD
                     DGNTVTDWMVQERERGITIVSAAVSAEWKGYQVNIIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVQGVEPQSETVWRQADRYGVPRICFVNKMDRVGASYERTIETIIDRLGAKPI
                     PMQLPIGFEATFKGVVDLLSMTAIVWEDDLGKEPKVTEIPADLKDQAVEARARMVERI
                     AELDDELTMKFLEAQEIGVDELKAALRKGVISNKATPIFCGSSLKNKGVQAMLDAVID
                     YLPSPMDKATITATEPGNPENTFEVPTVDDAPLSALVFKIVTDPYVGRLAYIRVYSGV
                     LSQGQTVQNSTKGKKERIGRLLKMHADRREDITEIRAGDIGAILGFKDSFTGDTLCDT
                     KSLALESISFPEPVISIAIEPKSSSDQEKMGEALRKLAEEDPTFRVNSDDSTGQTVIR
                     GMGELHLDIIVDRLLREFKVQANVGTPRVAYRESITKPVKEVNYKYAKQSGGKGQYGH
                     VVFALEPGERGSGIIFENKIVGGSIPKEYINPIEKGFREACESGILAGYPVVDLKITL
                     FDGSFHEVDSSEMAFKLAASMGFKEGMQKGNAILLEPMMKVEVVAPEEYLG"
BASE COUNT          643 a          548 c          625 g          657 t
ORIGIN      
        1 gatctgcctg gtgtgcgcta tcacatcgtc cgcggaacct tggacacgac tggtgtggcc
       61 aatcgtaagc agggccgctc aaagtacggc gcgaagcgtc caaagtaaaa tgatggagta
      121 gttgaaacat gcgtagagca aagccagaaa gacgggagat tctccccgat atccgcttta
      181 atagcatcaa tattcagact atgattcagc acgttcttaa gaatggaaag aagagtgttg
      241 ccacaggtct gatttatgat gcgatggatt tgatcaagga aagaactgag aagaatccaa
      301 tggatgtgtt tgacatcgcc ctaaaaaatg tggggcctgt tatggaagtc cgcccacgcc
      361 gtgtgggtgg tgctacttat caggttccaa tggaagtttc tgctgaccgc cggaccactc
      421 ttgcaattcg ctggatactc ggtgcagcac gagatcgctc tggtaaatcc tttgcggata
      481 agctcgcgtc tgaattgatt gatgcatcca atgaaactgg tgctgcaatt cgcaaacgtg
      541 acgaagctca caaaatggca gaagccaacc gtgctttctc ccattatcgt atttaatctt
      601 ataaaggtag atagatagca atggcacgcg ttcatccgtt ggagaagtat cgaaatattg
      661 gcattattgc ccacattgac gccgggaaga caacaactac cgagcggatt atgttctata
      721 ccggtttaac tcaccgcatc gggtctgttg acgatggcaa taccgtaacc gactggatgg
      781 tgcaggagcg tgaacgcggt attaccattg tatccgccgc agtctctgcc gagtggaaag
      841 gttatcaagt taacattatt gataccccgg gtcacatcga ctttactgct gaagttcagc
      901 gttcccttcg tgttctcgat ggcggcgttg tagttttcga cgctgttcaa ggcgtggaac
      961 ctcagtctga aaccgtttgg cgtcaggctg accgttatgg tgttccacgt atttgttttg
     1021 ttaataagat ggaccgtgtt ggtgcttcct atgaacgcac catcgaaacc attattgacc
     1081 gcctcggcgc caagccgatc ccgatgcaac ttcccattgg ttttgaagct accttcaaag
     1141 gagttgttga ccttctttcc atgactgcaa ttgtctggga agatgacctc ggcaaagagc
     1201 ccaaggttac tgaaatccct gcggatctaa aggatcaagc tgtggaagcc cgtgccagaa
     1261 tggttgagcg aatcgccgaa cttgatgacg aactcaccat gaaattcctt gaagcacaag
     1321 agattggcgt ggatgaactc aaagccgctc ttcgaaaagg tgtgatttct aataaagcaa
     1381 cgcctatttt ctgtggttct tccttgaaaa ataaaggcgt tcaagctatg cttgatgcag
     1441 taatagatta tctcccttct cctatggata aggcgacgat tactgcaact gaacctggta
     1501 atccggaaaa cacttttgaa gtcccgactg ttgatgatgc accactgagt gcacttgttt
     1561 tcaagattgt tacagatcct tatgtcggtc gtcttgctta tatccgcgtt tactcgggtg
     1621 ttttgagcca gggacaaact gttcaaaata gcaccaaagg caagaaggaa cgcattgggc
     1681 gtttattgaa aatgcatgca gaccgcagag aagatattac tgagattcgt gctggtgata
     1741 ttggtgctat tttgggtttc aaagatagtt tcactggcga taccctttgc gatactaagt
     1801 cattagctct tgaaagcatt tcgttccccg aacctgttat ctccatcgcc attgagccga
     1861 aaagctcaag cgaccaggaa aagatgggtg aggcgcttcg aaaacttgct gaagaagatc
     1921 ccacattccg tgttaattca gatgatagca ctggtcagac cgttatccgt ggtatgggcg
     1981 agcttcatct cgatattatc gttgaccgtc tcctgcgcga gttcaaagtg caggcgaatg
     2041 ttggtacgcc acgtgttgcc tatcgcgagt cgattaccaa gccggtcaag gaagttaatt
     2101 acaaatatgc caaacagtcc ggtggtaagg gtcaatacgg ccatgtcgtg ttcgctcttg
     2161 aacctggtga acgtggcagt ggcattatct tcgaaaacaa gatcgtcggt ggttctattc
     2221 cgaaggaata catcaaccca atcgaaaaag gtttccgtga agcttgtgaa agcggcattc
     2281 tcgctggtta cccggttgtg gatttgaaga ttaccttgtt cgacggctcg ttccatgagg
     2341 ttgactccag cgaaatggca ttcaaacttg ccgcttccat gggttttaaa gaaggtatgc
     2401 agaagggcaa tgccattctg cttgaaccga tgatgaaggt tgaagtagtt gcgccggaag
     2461 aatatcttgg cga
//
LOCUS       DAOQFT010000383         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27219_length_2470_cov_6.573913, whole genome shotgun sequence.
ACCESSION   DAOQFT010000383 DAOQFT010000000
VERSION     DAOQFT010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27219_length_2470_cov_6.573913"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /gene="mraW"
                     /locus_tag="PLR93_09725"
     CDS             <1..301
                     /gene="mraW"
                     /locus_tag="PLR93_09725"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNH27288.1"
                     /translation="AVNDELAAVEKTLPQAVASLNTGGRCAIITFHSLEDRIVKDYFR
                     EQSKDLINPPYERIYEVERKAVVKLVNKKPILPTEEETRENPRARSAKLRVVEKL"
     gene            342..821
                     /locus_tag="PLR93_09730"
     CDS             342..821
                     /locus_tag="PLR93_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27289.1"
                     /translation="MNIPNVNLPNVTHVIHAYRVAPWRVQRQWIGNALLVVVSLAMVA
                     ALYLNVTSRTAIAGREIQDLTEAISASQQVSSDLQTQLANLTSAEVMQQRAKEMGFRP
                     VEPVELEYLVVPGYVAKDAVNLSSSAQLQLNVLAIPPEYNQSLLDWMDERLVARRYQ"
     gene            818..>2470
                     /locus_tag="PLR93_09735"
     CDS             818..>2470
                     /locus_tag="PLR93_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNH27290.1"
                     /translation="MRQQYGRRSQVLAAVLAFFAFAIVFQMTRIQNSPEADTFRQLSK
                     DYSYEWRTFYPNRGEIYDRNGRLLAGEKIVYEIGVDLNIMTDAHAIAFAVADALGMNY
                     DNLMSVIQNPPDGLSYIVLADYVESSQANYLQDLKKALNEQAPEGSMGGLTGLQFKSH
                     PQRSYPENSLASNILGFVNREGKGYFGVEEKYDSLLAGNPVTVLVPTDPNKAFEIPNA
                     PDGTTLVLTINRDLQSAAEEILDDALGTYGAQDGIIAIMDPQSGEMLAVATSRRMDLN
                     EFWNYGTIYNNATEFNPIISKTYEPGSVMKILTMAAALDSNTVTPETTYLDTGEILIG
                     GVTIQNWDRNPWGVQNMVGCLQHSLNICMVTISSQMGANTFYKYMDAFGIGHLTGVDL
                     AWEAPGRLKVPGDADWYPVDLGTNSFGQGVAVTPIQMMSAVSAVANNGRMVIPHVLYA
                     MVRDGRQYEVPDQFGPTPISEQTAHTLSEMLAVSLESESSAALIPGYRVAGKTGTAQI
                     PVDGFYDSTQTNTSFIGWGPVDDPKFMIYVWLERPTASPWGSE"
BASE COUNT          634 a          595 c          599 g          642 t
ORIGIN      
        1 cgccgtcaac gacgaattgg ccgcagtgga gaaaacgctt ccgcaagccg tggcaagcct
       61 gaacaccgga ggtcgatgcg caatcattac cttccattct ttggaagacc gcatcgtcaa
      121 agactatttt cgagagcaga gcaaagatct catcaatccg ccttacgaac gaatttatga
      181 agtggagcgt aaggctgttg tgaagttagt gaataagaag ccgatcctgc cgaccgagga
      241 agagactaga gaaaaccctc gtgcccgcag tgccaaactt agagttgttg agaagttgta
      301 ggggcgaggt cttcctcgct tgcaattgag agagaattaa agtgaacata ccgaacgtca
      361 acctccccaa tgttactcac gtgatccatg cctaccgggt cgctccttgg cgcgtgcaac
      421 gccaatggat cggcaatgcg ttattggttg tggtgagcct tgccatggtt gccgctcttt
      481 atttaaatgt aacgtctcgc actgctattg ctggtcgtga aattcaagac ctgaccgaag
      541 ccatctctgc cagccaacag gtgagtagcg atcttcagac ccaactggca aacctgactt
      601 ctgctgaagt catgcaacaa cgtgcaaaag aaatgggttt ccgacctgtg gaacctgttg
      661 aactggaata tcttgtggtg ccgggctatg ttgctaaaga cgcagtcaat ctttcctcct
      721 ctgctcaact tcaattgaat gtgcttgcca ttccgcctga atacaatcag tcacttttgg
      781 attggatgga tgaacgcttg gtggcacgga gatatcaatg agacagcaat acgggcgccg
      841 cagtcaagtc ttagctgccg tattggcttt ctttgcgttt gccatcgtgt ttcagatgac
      901 gcgcattcaa aacagccccg aagccgatac ctttcgccag ctttctaaag attattcata
      961 cgaatggcgc acgttttacc ccaaccgcgg cgagatctat gaccgtaacg gtcgcttgtt
     1021 ggctggggag aagatcgttt atgaaattgg cgtagactta aacattatga cggatgctca
     1081 cgccattgca tttgcagtag cagatgctct tggtatgaat tacgacaatt tgatgtcggt
     1141 cattcaaaac ccaccagatg ggctctcata cattgtcctt gcagattatg ttgagtcttc
     1201 acaagcgaat tatttgcaag acctcaaaaa agcattgaac gaacaagccc ctgaaggttc
     1261 aatgggcggg ttgacgggct tgcagtttaa atctcaccct caacgtagtt atcccgaaaa
     1321 ctcactggct tcaaatatcc ttgggtttgt taatcgtgaa ggcaagggat acttcggtgt
     1381 agaagagaaa tacgatagtt tattagcggg caatcctgtc actgtattgg tgccgacgga
     1441 cccaaacaaa gcctttgaaa ttcccaatgc gcctgatggc actacattgg ttcttaccat
     1501 caaccgcgac ttgcagtctg ccgcagaaga aattcttgac gatgctttag gaacttacgg
     1561 cgcacaagat ggcattatcg ccattatgga tccacaaagc ggcgagatgc ttgccgttgc
     1621 tacatcgcgc cgtatggatt tgaatgaatt ttggaattac ggcactattt ataacaatgc
     1681 aaccgagttt aacccgatca tttccaaaac ctatgaaccc ggttccgtga tgaagatcct
     1741 tacgatggct gccgctttag acagcaatac tgtaacccct gaaacgactt atctcgacac
     1801 tggtgagatt ctgatcggcg gtgtgaccat tcaaaactgg gatcgcaatc cgtggggtgt
     1861 gcagaacatg gtggggtgtt tgcagcattc actcaatatt tgtatggtca ccatttcttc
     1921 acaaatgggt gcaaatactt tctataaata catggacgct ttcggaattg gtcacctcac
     1981 cggcgtggac ttggcttggg aagcgccagg caggctcaag gtcccgggag atgcggattg
     2041 gtatcccgtg gatcttggta caaactcatt tggtcaaggt gtagcagtca ctcctatcca
     2101 aatgatgtct gccgtttctg cagttgccaa taacggacgc atggtcatcc cacatgtttt
     2161 gtatgccatg gtacgcgatg gtcgtcaata cgaagtgcca gatcagttcg gtcccacccc
     2221 catctcagaa cagacagcac atacattatc tgaaatgcta gcggtttctc tcgaatcaga
     2281 atcatctgct gcattgatcc ctgggtatcg cgtggctggt aagaccggta cagcgcaaat
     2341 tccagtcgat ggtttttacg acagcacgca aacaaatacc tctttcatcg gttgggggcc
     2401 agtagatgac ccgaaattca tgatctatgt ttggctcgaa cgccctacag cttcaccttg
     2461 gggttctgaa
//
LOCUS       DAOQFT010000384         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27227_length_2470_cov_4.087785, whole genome shotgun sequence.
ACCESSION   DAOQFT010000384 DAOQFT010000000
VERSION     DAOQFT010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27227_length_2470_cov_4.087785"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLR93_09740"
     CDS             complement(<1..841)
                     /locus_tag="PLR93_09740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNH27291.1"
                     /translation="MISLVDLTAQYHSIKKEIDTAVLSTLESGHFILGPQVVKFEESI
                     AAYLGVDHAIGLASGTDALVIALRALNIGDGDEVIIPAYTFFATAGTVMSVGAKPVIV
                     DVDPQSYQIDVSKIEAAITPKTKAIIPVHLYGHPAEMNPILEISRKHGLKVIEDNAQG
                     FGAEYLGKKTGSFGDIGCLSFFPTKNLGAYGDGGAVVTNDPVLAEQMRMLRAHGWKKK
                     YYSEMVGYNSRLDAMQAAILQAKFPYLDGWNEKRRELSKRYTEHLAPLGIVTPVEREW
                     AKHV"
     gene            complement(838..1419)
                     /locus_tag="PLR93_09745"
     CDS             complement(838..1419)
                     /locus_tag="PLR93_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNH27292.1"
                     /translation="MSDYFVHESSYVDEGAEIGVGTKIWHFCHVMPHAKIGERCNIGQ
                     NVLVSSDVIIGTNVKIQNNVSLYTGVIVEDDVFLGPSMVFTNVINPRSHVSRKDEYKT
                     TLVRKGASIGANATIVCGTTLGRYCFVGAGAVVTKDVPDYALVYGTPARVRGWVCQCG
                     EQLTFDADHAVCSRCGDAYRKQDSVVSPERGES"
     gene            complement(1422..2363)
                     /locus_tag="PLR93_09750"
     CDS             complement(1422..2363)
                     /locus_tag="PLR93_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNH27293.1"
                     /translation="MAKNFAVIGVGGYIAPRHLRAIKDTGNRLLAAVDPKDSVGVLDQ
                     YSFDIRFFTEIERFDRYLEKLRRGPEEERVHYVSVCSPNYLHDAHSRLALRVGADVIC
                     EKPLVINPWNLDALEEIEADTKHRVHTILQLRVHPSLIQLRESLKDSNEIHEVELTYI
                     TSRGPWYQVSWKGDYEKSGGVATNIGIHFFDLLMWLFGAVNGIKVYHKDEERMSGFIE
                     LERARVRWFLSVDKNDLPIHAKANNKTTYRSITVDGKEIEFSEGFTELHTKVYEETLA
                     GRGFGIKDARPSVQLTYDIRKAPISPLDTLAHPLLKK"
BASE COUNT          668 a          660 c          497 g          645 t
ORIGIN      
        1 aaacatgttt cgcccattcg cgctcaacag gtgtgacgat ccccagcggc gcaagatgtt
       61 cggtataccg ctttgaaagc tcacggcgct tttcgttcca tccgtccaga tatggaaact
      121 tggcttgcaa gatcgctgcc tgcatcgcat caagacgact gttataacca accatctctg
      181 aataatattt cttcttccag ccgtgcgcgc gtaacatgcg catctgctcg gcaagcaccg
      241 ggtcgttggt caccaccgcg ccgccgtcgc catacgcacc caggttcttg gtcgggaaga
      301 aactcaaaca gcctatgtcg ccaaacgaac cggtcttctt cccaaggtat tctgcgccaa
      361 atccctgcgc gttatcctca atgactttca gcccatgttt gcgggaaatc tccagaatcg
      421 gattcatctc cgcgggatgc ccgtacaaat ggacagggat gattgccttc gtcttcggcg
      481 taatcgcagc ttcgatcttg ctcacatcaa tctgataact ctgcgggtct acatccacaa
      541 tcaccggctt tgcaccaacg gacataaccg tgccagccgt ggcaaaaaat gtataagcgg
      601 ggataataac ttcatcacca tcgccaatat tcaaggcacg taatgcaatc accaatgcat
      661 ctgttcctga agccagccca atggcatgat caacgccaag gtatgcagcg atgctctctt
      721 cgaacttcac cacctgcgga cctaaaataa aatgacccga ctccaaagtg gataaaactg
      781 ccgtatcgat ttcctttttg atggaatggt attgcgccgt gagatcaacc aacgagatca
      841 tgattcgcct ctttccgggc tgacaactga atcctgtttt ctgtaagcgt ctccgcacct
      901 tgagcataca gcgtgatcag catcaaaggt caactgttca ccacattgac atacccaccc
      961 gcgcacacga gccggggtgc catacaccaa cgcataatcc ggcacatctt tcgttacgac
     1021 cgcgcccgca ccgacaaaac aatatcgtcc gagtgtcgtg ccgcagacaa tggtcgcatt
     1081 cgctccgatc gaagcgcctt tccgcaccaa tgttgtttta tattcatcct tgcgactaac
     1141 gtggctgcgt ggattaatca cattcgtaaa aaccatcgat ggtccaagaa atacatcgtc
     1201 ttcaactatc acaccggtgt aaagagagac attattctga atcttcacat tcgttccaat
     1261 aattacatca gaagagacca acacattctg accaatgttg caacgctcac cgatcttggc
     1321 atgcggcatt acatggcaaa aatgccatat tttcgtcccc acaccaatct cggcaccttc
     1381 atcaacataa ctggattcgt gaacaaaata atcagacatt tttatttctt cagtaatgga
     1441 tgtgccaatg tgtctaatgg agaaattggc gctttgcgaa tatcataggt caactgcacc
     1501 gatggacgtg catctttgat gccaaatccg cgtccagcaa gggtttcttc ataaactttc
     1561 gtatgcagtt ctgtaaaacc ttctgaaaac tcaatctctt taccatccac tgtaatggat
     1621 ctgtaggtgg tcttattatt cgcctttgca tgtataggca ggtcgttctt atctactgaa
     1681 aggaaccaac gcactcgtgc tctttccaat tcaataaagc cagacatacg ttcttcatcc
     1741 ttgtgataaa ccttaatacc atttacggcg ccaaataacc acatcaacaa atcaaagaaa
     1801 tgaataccaa tgttggttgc taccccgcca gatttttcat aatcaccttt ccatgaaact
     1861 tgataccaag gtccacggct tgtgatgtac gtcaattcaa cttcatgaat ttcattagag
     1921 tctttcaatg actcacggag ttgaatcagg cttgggtgga cgcgcagttg taagatcgta
     1981 tggacgcggt gttttgtatc cgcttctatt tcttcgagag catccaaatt ccaagggttg
     2041 atcacaagtg gcttttcaca gattacatct gcacctactc gtaaagcgag acggctatgt
     2101 gcatcatgca aataattggg cgaacaaacc gaaacatagt ggacgcgttc ttcttctggt
     2161 cctcgacgaa gcttttcaag ataacggtca aagcgttcga tttcagtaaa aaaacgaatg
     2221 tcaaaagaat attgatcgag tacaccgacc gaatctttcg ggtctacagc tgcaagcaaa
     2281 cggttgcctg tatcttttat ggcacgcaaa tgtcgagggg caatataccc acccacaccg
     2341 atgacagcaa agtttttagc cataattcct tttctctatt tcgaacgtac atgataccca
     2401 tgcccggttg cttcgagcac acagtcttcg ggcaggtcgc tgatctctaa accagcttca
     2461 agacaagtga
//
LOCUS       DAOQFT010000385         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27256_length_2468_cov_6.406548, whole genome shotgun sequence.
ACCESSION   DAOQFT010000385 DAOQFT010000000
VERSION     DAOQFT010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27256_length_2468_cov_6.406548"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..940
                     /locus_tag="PLR93_09755"
     CDS             17..940
                     /locus_tag="PLR93_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNH27294.1"
                     /translation="MSDLTPILVTGSHRSGTTWVGRMLAADASTAYISEPLNVHHRPG
                     VYRTLVKYWYTYITDENGADYLPAFNELLDFKYHTVRETLALRSFKDFLRMGRDFHTF
                     FNGSMQGRRILIKDPFAIFSTPWFEKQLHCKVVITVRHPGGFVSSLKRLGWNFDFNNF
                     LSQPLFMRDHLEGDRTAMEAMSGDDLVGQAALLWKIIYRFVHGARSQFPQFHIVRHED
                     LSRDPINGYQALYQSLHLDFTERVKDIIQNSSSSENPTKLAKNKTHSVKLDSRANLDN
                     WKKILSPEEITRVRKITEGVSESFYSDEEWK"
     gene            965..1744
                     /locus_tag="PLR93_09760"
     CDS             965..1744
                     /locus_tag="PLR93_09760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH27295.1"
                     /translation="MKPASLPLVTIVTPSFNQADYLEATIKSVLEQSYPHIEYIIMDG
                     GSTDGSVDIIKKYEGKLGYWESVKDKGQTDAINKGFNRAKGDILAWLNSDDTYANPNA
                     IADAVNFLVANPEVAMVYADCDFINEQGQVIGKFASRQTDYAKLRTGYVHIPQQTMFF
                     RARYWKELGPLDPSFYFAMDYDLWVRIAKHAPIQYLPGKTWANFRIHTSSKTNVNDER
                     GWKEMLRVHYRDGGSFFSSIVAKYYLRKIIGPLWKWRIKKS"
     gene            1766..2368
                     /locus_tag="PLR93_09765"
     CDS             1766..2368
                     /locus_tag="PLR93_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27296.1"
                     /translation="MNFLFFSPSLDDIIRLLKAWRFWVLGAIIGSAIGVAFYDIAPPP
                     YRAKAIVNVDFNFEEAFPANTDRQDFYYLEREARKMVELAWSDDVLNELNAPVKELRE
                     GKLTLSQPAEAGWHFYADDQNPQKAEELASAWANAFVEKAQAEIQAGSLNKFIKLEVT
                     QSANLPKQRSVPLANYLLAGSSGFLLLAMFVVLFIKPKAK"
BASE COUNT          630 a          621 c          555 g          662 t
ORIGIN      
        1 cttatggagg cactaagtga gtgacctaac ccctattctc gtcactggct cgcatcgtag
       61 cggaaccact tgggttggta gaatgcttgc tgccgatgca agtactgcat atatcagcga
      121 accgcttaat gtacatcacc gtcccggtgt atatcgcacg ttggtcaagt attggtatac
      181 ctacatcacg gatgagaacg gcgcagacta tctacctgct ttcaacgagc ttcttgattt
      241 taagtatcat acggtgcgtg aaaccctcgc actgcgttca ttcaaagact ttctgcgcat
      301 gggacgggac ttccatacat ttttcaatgg cagcatgcaa ggacggcgaa ttctgatcaa
      361 agatcctttt gctatattct ctacaccgtg gtttgagaag cagttacatt gcaaagtggt
      421 catcacagtt cggcatcctg gcggttttgt tagcagtctc aaacgccttg gctggaattt
      481 tgatttcaac aattttctta gtcagccgct ttttatgcgc gatcatttgg aaggtgaccg
      541 caccgccatg gaagccatgt caggcgatga ccttgttggg caagctgcat tgctgtggaa
      601 gatcatttac cgcttcgtac atggggcccg aagccagttc ccccaatttc acattgtgcg
      661 ccacgaagac ctttcccgcg accccatcaa cgggtatcaa gccttgtatc agtccctcca
      721 tttggatttc actgaacgcg taaaggacat tatccaaaat tccagtagct cagagaatcc
      781 gaccaagctg gcaaagaaca aaactcattc tgttaaactt gacagccgtg ctaacctcga
      841 taactggaag aagatacttt ctcccgaaga gataactcgt gtgcgcaaga tcaccgaagg
      901 cgtttctgag tctttctatt cggatgagga atggaaatag caattgcctg acagagctac
      961 ctgaatgaaa ccagcctctc tccctcttgt taccatcgtt actccatcct tcaatcaagc
     1021 agactacctc gaagcgacga tcaaatccgt gctggaacag tcgtatccgc acatcgaata
     1081 catcatcatg gacggcggct cgaccgatgg cagtgtggat atcatcaaaa agtatgaagg
     1141 taaattaggc tattgggaat cggtgaaaga taaaggacag accgatgcca tcaacaaagg
     1201 cttcaaccgt gccaaaggcg atatccttgc ctggcttaac tcggatgaca cttatgccaa
     1261 ccccaatgcc attgccgatg cggtgaattt tctcgtggcg aatcctgaag ttgccatggt
     1321 ctatgccgat tgtgatttca tcaatgagca aggacaggtc attggtaagt ttgcctcgcg
     1381 ccaaacggac tatgccaaac tacgcaccgg ctacgttcac atcccccaac agaccatgtt
     1441 cttccgtgcc aggtattgga aggagcttgg tccgcttgac ccttcattct attttgcaat
     1501 ggactacgac ctgtgggtgc gtattgccaa acatgccccc attcaatacc tgcctggtaa
     1561 aacttgggcg aattttcgaa ttcatacttc cagcaaaacc aatgtcaacg atgaacgcgg
     1621 ttggaaggaa atgctgcgcg tccattatcg tgatggcggc tccttctttt cctcaattgt
     1681 ggcaaaatac tacctgcgca aaataatcgg tccgctgtgg aaatggcgca tcaaaaaatc
     1741 gtagatcgac aagtacaaat ctaaaatgaa ttttctcttc ttctctcctt ctctcgatga
     1801 catcattcgc ctgctcaaag cctggcgttt ttgggtattg ggcgcgatca ttggctctgc
     1861 cattggcgtt gcgttctatg acatcgcccc gccgccgtat cgtgccaaag ccattgtcaa
     1921 cgtagacttt aatttcgaag aagcttttcc agccaataca gaccggcaag acttttacta
     1981 tcttgagcgt gaagctcgca aaatggtgga gcttgcctgg tcagacgatg tgttgaatga
     2041 attgaatgct cctgttaaag aactgcgtga aggaaagttg accctttctc aacctgctga
     2101 agctggctgg catttttatg ccgatgatca aaacccgcaa aaagcagaag aacttgcttc
     2161 agcatgggcg aatgcctttg ttgaaaaagc ccaagccgaa attcaggcag gcagcctgaa
     2221 caagtttatc aagcttgagg tgactcagtc tgccaacctg cccaaacaac gcagtgttcc
     2281 acttgccaat tatctgcttg caggctcaag cggatttctc ttgcttgcca tgtttgttgt
     2341 tctgtttatt aaacccaaag ccaaatgaag aattctgttt tttcaataga taacctcccg
     2401 cgcctattat ggggactggc tttactaacc ctgcctgtca ccagttttcg gtggttccct
     2461 ggtttggg
//
LOCUS       DAOQFT010000386         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27334_length_2464_cov_6.216272, whole genome shotgun sequence.
ACCESSION   DAOQFT010000386 DAOQFT010000000
VERSION     DAOQFT010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27334_length_2464_cov_6.216272"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLR93_09770"
     CDS             <1..857
                     /locus_tag="PLR93_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH27297.1"
                     /translation="TANIPMLIDFIGKAFNDRTPFPAEVYFVSKKEFANKKWGTSQPI
                     LVETPGKGLGYLFIQGFGTFSFQVKDPQQFVTQIVGATGTYRTSEIEERLKTMLVSKL
                     TDVLGEFSQTTQSLAAKISGMNEELGAAVRAKGQADFEAIGLTLKTFYIGNLKPSTKS
                     AQELRDAGLLDPALYAQLQAADAMREAANNPNGGAGLTAGIGAGMGIGNLMGQALQGG
                     MQTGGAPAAGGGSKMPDIMTPAEAAQFMKVTEDDIMAAIQDGSLKAKKVGKAFRISKD
                     NLESFMNS"
     gene            complement(911..>2464)
                     /locus_tag="PLR93_09775"
     CDS             complement(911..>2464)
                     /locus_tag="PLR93_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNH27298.1"
                     /translation="MKLVTVSQMRAIEKEADAKGISYAQMMQNAGCGLAEIVHVIGQE
                     HGWTNVVGIVGSGNNGGDALVALTWLAKAGWKTQAYVVKRQDADEELVKKYLDAGGQI
                     LQHADDVSLNTLRDFLEDVDILIDGLLGTGIKLPLRKDASLVLQEISHLLLERKHSPF
                     IVAVDCPSGIDCDTGECADETLHADLTITMAAVKQGLLKLPAYEYIGKLELVDIGLPK
                     DLDSWDALQIDVADADIVSAILPKRTPASHKGTFGTAFVVAGSTSYTGAALLAGSAAY
                     RIGAGLVTMAIPAPLHTALVGHLPEATWVPLPHEHGFISEEASYYVLSNLERATSILI
                     GPGFGNKHTTKLFMEHLLDFLELPSVIDADGLRHLAGIHDWYKKIKVQAILTPHPGEM
                     SALTGLSISEIQSNRQEVALKYAKAWGHVVVLKGAFTVIASPSGCATIIPVATAALAR
                     AGTGDVLAGLIAGLRAQGVEAYEAAIAGTYIHAQAGLYAADRLGTTASVLASDVLEAI
                     PRVISELEQ"
BASE COUNT          689 a          622 c          526 g          627 t
ORIGIN      
        1 cgactgccaa catcccgatg ttgatcgact tcattggcaa ggcgtttaat gaccgtaccc
       61 ccttccccgc cgaagtttat ttcgtttcaa agaaagaatt tgcaaataaa aaatggggta
      121 cttcacaacc cattcttgtg gaaactccgg gcaaaggctt gggctacttg ttcattcaag
      181 gctttgggac tttctccttc caggtgaaag acccgcaaca atttgtgaca caaatcgttg
      241 gtgcaacagg aacatatcgc acatctgaaa tcgaagaacg tcttaagact atgctggtct
      301 ccaagttgac cgacgtgctc ggtgaattca gccagaccac ccagagcctt gctgctaaaa
      361 tcagcggcat gaacgaggaa ctcggtgcgg ctgtccgcgc taaggggcag gctgattttg
      421 aagccattgg cttaacactc aagactttct acattggcaa cctcaagcct agcaccaagt
      481 ctgctcagga attgcgcgat gctggtttgc tcgaccccgc tctttacgct caactgcaag
      541 ctgcagatgc catgcgcgaa gccgcaaaca acccgaatgg tggagctggt ctcaccgctg
      601 gtattggcgc cggaatgggc attggtaacc tgatgggaca agcccttcag ggtggtatgc
      661 aaactggtgg cgctcccgcc gctggtggtg gatcaaaaat gcctgatatc atgactcctg
      721 ctgaagcagc tcagttcatg aaggtcaccg aagatgacat catggcagcc attcaagacg
      781 gaagcctgaa agccaagaaa gtaggtaagg cgttccgcat tagcaaggat aaccttgaat
      841 cttttatgaa tagctagttt ataaggaaaa aaaaagaggc tttcattacg aaagcctctt
      901 tttttttatt ctactgttcc aactcagaga tgactcttgg aattgcctct aacacatcac
      961 tggcaagaac cgaagctgtt gttccaagtc tatcagcagc atatagccca gcttgtgcat
     1021 gaatatatgt ccccgcaata gccgcttcgt atgcttctac accttgtgca cgaagaccgg
     1081 ctattaaacc tgccaacaca tcccccgtcc ctgctcgggc aagggctgct gtagcaaccg
     1141 ggattatggt tgcacaccca cttggggaag caataaccgt aaatgcgcct ttcaatacaa
     1201 caacatgtcc ccatgccttt gcatacttta aagcgacctc ttgccgatta gattgtatct
     1261 ctgaaattga taaccctgtt agcgcagaca tctcgccagg atgtggagtt aggatagcct
     1321 gcacttttat ttttttatac caatcatgaa ttccagcaag gtgccgcagt ccgtctgcat
     1381 caataacaga tggcaattcc agaaaatcca ataaatgttc cataaacaat tttgtcgtgt
     1441 gcttgttccc aaaacctggt ccgatcaaaa ttgaggttgc acgctccaaa ttcgacaaaa
     1501 cataatagct ggcttcctct gaaataaatc catgttcatg tggcaatgga acccaagtcg
     1561 cttcggggag atgaccaaca agcgcagtat gaagcggtgc gggaattgcc attgtgacca
     1621 atcctgcacc aatacgatag gcagcactgc cagccagcaa agccgcgcct gtataggatg
     1681 tagagccagc caccacgaag gcagtaccaa atgtcccttt gtgagaagcg ggcgttcgct
     1741 ttggtaagat ggcagaaaca atatcagcat ctgcgacatc aatctgcaaa gcatcccaag
     1801 aatccaaatc tttgggtaac ccaatatcca caagttctaa tttaccgatg tactcatatg
     1861 cagggagttt gagcaagcct tgttttactg ctgccatcgt aattgtaagg tctgcatgca
     1921 aagtttcatc ggcacactca ccagtatcac aatcaatgcc agatggacaa tcaaccgcga
     1981 cgataaaagg tgagtgtttt ctttcaagta agagatggga tatttcttgc aaaacgagag
     2041 atgcatcttt cctcaacggt agtttgatgc ctgtgccaag caacccatcg ataagaatat
     2101 ctacatcttc tagaaaatct ctcaaagtat tcaagctaac gtcatcggca tgttgtaaaa
     2161 tttgaccgcc agcatcaagg tattttttaa ctaattcttc atctgcatct tgacgtttca
     2221 ccacgtaggc ttgagttttc catcctgctt tggcaagcca agttaaagca acgagtgcat
     2281 cgcccccatt attccctgaa ccaacaatcc ctacaacgtt agtccaacca tgctcttgtc
     2341 caatcacatg aacaatctca gccaaaccac acccagcatt ttgcatcatc tgagcataag
     2401 agatgccctt tgcatcagct tctttttcaa ttgctcgcat ttgagaaaca gtgacaagtt
     2461 tcat
//
LOCUS       DAOQFT010000387         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27703_length_2442_cov_7.162547, whole genome shotgun sequence.
ACCESSION   DAOQFT010000387 DAOQFT010000000
VERSION     DAOQFT010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27703_length_2442_cov_7.162547"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1525
                     /locus_tag="PLR93_09780"
     CDS             344..1525
                     /locus_tag="PLR93_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27299.1"
                     /translation="MRIMNKKLQVCLIAPALIAVMAFVFLLSGCVVSKKKLLPDGNYL
                     VLKAPIKPGYYLGHDLSTTNGVRQFKFDGIPYLMTLENGTYTLAKGGFTKQSKTLNGQ
                     LFASQDENEIFIAQSQSDQIWQYQRVDVGDDSFVITEQHCSRLDKQYINWLIKQKTIS
                     KYESGFCYVNDTINLSALILLADNWKGAPKTFYLAADDKSPIESSRLEDKLFVDPMCD
                     GDPVQALGPGFQGTNQARVGMHMVIMAASTDGPLSMNAVAIEANEGKVGNWAWLSPWL
                     VDNKISCKPHKLVNIWAFRPDKNAFSSVTMSASEVNSGCKHYGYPRTQCGSVNEIVDR
                     LRKRGMHILLQGEEYVWYPNGRYGKKGAYITVMTDVEGLKNKYEVVYTLSDGLSFIYS
                     K"
     gene            2215..>2442
                     /locus_tag="PLR93_09785"
     CDS             2215..>2442
                     /locus_tag="PLR93_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27300.1"
                     /translation="MNYATGETVQVGDHVLIERGRTPGRVREVIEADTDLSQWNIEEP
                     GLMVEAAPFGLVFWPASSPDAVVLVGRGRKGY"
BASE COUNT          609 a          475 c          709 g          649 t
ORIGIN      
        1 tgagccgcag tgcgcgggtg ctgatggaag gatgggtgcg ggtgccgggg gatacattct
       61 gatcggcgtc tgacgtttta cataaaacag ggaaaagccc tgccgcggtg cggcggggct
      121 tttttgtttg tgctgtgtgt tcactatcgt tacattcaca tcgcgacttc tgtagttgtc
      181 gccaagtcat ccatgtaggg tatgcgatat tcttgagtgc gcggattgtc cttgttaagg
      241 agtgtgagca aataaatgct aatcctatgg taattcagtt ctttgttttt gtcgctaagc
      301 gaagctttga atagaaaata ggaactctat ggggggttga tctatgcgca tcatgaataa
      361 aaaattgcaa gtgtgtctta ttgctcctgc tttgattgca gttatggctt ttgtttttct
      421 tctttcgggg tgcgttgttt caaaaaagaa attgctgcca gatggcaatt atttggtgtt
      481 aaaggcgcct ataaagccag gatattattt agggcatgat ctctcaacca caaatggtgt
      541 gcgtcagttc aagtttgatg gaatccctta tttaatgact cttgaaaatg gaacatacac
      601 actcgccaag ggaggattca caaaacaaag caaaacattg aatgggcagc tatttgcgag
      661 tcaggatgaa aatgagattt ttattgctca gtcccagtct gaccaaatct ggcaatatca
      721 gagagtggat gtgggtgatg attcgtttgt gataacggag cagcactgca gcaggcttga
      781 taagcaatat ataaactggc tgatcaagca aaaaacaatc agcaagtatg aatccggttt
      841 ttgttatgtg aatgacacca taaatctttc ggcgttgata ttgcttgcag ataattggaa
      901 aggtgccccg aaaacttttt atttggctgc ggatgataag tcgccgatcg agtcgagcag
      961 attggaagat aaattgtttg ttgatccaat gtgtgacggt gatccggttc aagcgttagg
     1021 gcctggcttt caaggaacga atcaagctag ggttggaatg catatggtga tcatggcggc
     1081 atcaaccgac gggccgttgt caatgaatgc tgttgcaatt gaagccaatg aaggcaaggt
     1141 tggaaactgg gcttggttgt cgccatggct tgtcgataac aaaatttcat gcaaaccaca
     1201 taagttggtc aatatatggg catttcggcc tgataaaaat gcattttcaa gcgtcactat
     1261 gtctgcgtca gaagttaact cagggtgtaa acactatgga tacccaagga cccaatgtgg
     1321 atctgtaaat gaaatcgtag accgacttcg taagcgtgga atgcatatcc ttctgcaagg
     1381 agaagagtat gtgtggtatc caaatggtag gtatggcaag aaaggcgctt atattaccgt
     1441 aatgacggat gttgaagggt tgaaaaacaa gtatgaggtt gtttacacgc tcagtgatgg
     1501 tttaagcttt atttattcca agtaaacttg attggttagc acgagtaggg cgggttaggc
     1561 gcgcactgcg ttgcatatca aagctcatca gcattgtcgc gcgccgtaac ccgccttctg
     1621 cgtttataat tatggcaaca cgaaagacat ggtgcgcatg gacagagttg tgcattttga
     1681 aaatctgtac ggcaatggca cggtcgcttt gtatggtaaa ttcccgaaat aaagcaggcg
     1741 tctggtgcgg tttggtgttt tttgctctgc ccggtgccac ggtgcaggcg gctgatggtg
     1801 agcaagcgta gggcaggtta gcgagtaggg cgggttaggc gcgtgggcaa tggctcaaag
     1861 tgggcgcctt gctcaatcgc gccgtaaccc gccttcgggg cgttacggca aggctttcgg
     1921 ggaaacaaag gcaggttttt ttgtgttcgc tgcttcgatg cgaggttcgc ggaaggcgga
     1981 ttacggtgcg cgggaaggcg tcagaactat ttggcgccgt gccatacgca cctaatccgc
     2041 cctacgcgtg ctgtattaga gaaggcatac tggaggatac agagccgcga gacgatgacg
     2101 acgatgaaca ttggagttag gcggaatgct tatgcccaac aaggcgctcc agccgacgta
     2161 tttgccgccg ctgcgcggcg tcaaattcgc ggctgagatg gggcgttggg cgtcatgaac
     2221 tacgcgacag gcgagaccgt gcaagtaggt gatcatgtcc tcatcgagcg aggtagaaca
     2281 cccgggcgcg tgcgcgaagt gatcgaagcc gacacggatc tgagtcagtg gaacattgaa
     2341 gaacctggtc ttatggtcga ggcagcaccg ttcgggttgg tcttttggcc tgctagttca
     2401 ccggatgcgg tcgttttagt cgggcgtggg cggaaggggt ac
//
LOCUS       DAOQFT010000388         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27823_length_2437_cov_5.560034, whole genome shotgun sequence.
ACCESSION   DAOQFT010000388 DAOQFT010000000
VERSION     DAOQFT010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27823_length_2437_cov_5.560034"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLR93_09790"
     CDS             complement(<1..262)
                     /locus_tag="PLR93_09790"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH27301.1"
                     /translation="MKFDPVLFAKAIADETRQKIMSECCCCELSVSEIVEKIGFSQPT
                     ISHHLAILRDANLVNVREEGKQTFYTLNQEHIAVCCGQIMVKF"
     gene            complement(345..2153)
                     /gene="pepF"
                     /locus_tag="PLR93_09795"
     CDS             complement(345..2153)
                     /gene="pepF"
                     /locus_tag="PLR93_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNH27302.1"
                     /translation="MTTPLPRSKVKKNQTWNAESVFKTPEAFDTELQNILADLPKIKK
                     YQGHLGENIEMFLEAFQAIDKLEKRTAKVRVYATMASAVDANDEQGAAMSSKAMSMLA
                     QVGAATSFVEPELLSMGEAKLRQWMKEDSRMKLYEHFLNDLFRKQAHVRNAEVEELLG
                     MVRDPFGAVRNTAGMLANADFKFKPAKDSKGRKLELTQSTRSALIESADRKVRQTSWE
                     NYNDKYLEFKNTLAGNLAASIKQNVFNMKARNFNSSLEATLFNGDVPVEMFHNLLDIF
                     KKKLPMWHKYWRIRRKALGVKVLHPYDVWAPLTTKKHKVPFETAVDWICEGLAPMGDE
                     YVETMRKGCLEDRWVDWSPNAGKREGAFSTRVPSDTHPFIMMSYTDDVGSMSTLSHEL
                     GHSIHAWYASRAQPMMYYLYPSIIAETASNFNQAMTRAHLLKTKKDKYFQIALLEEAM
                     DNFHRYFFIMPTLARFELETHQRAEKGQSLTADYMNNLMADLFSEGFGGEMHLDRERV
                     GITWGTFTTHLYIDYYSFQYAIGISAANAIAKRVLDGVPGAAQDHINFLKAGSSMSPM
                     EVYKTAGIDMTSTQPIEDAFAVLEEYIDRLGELTGK"
BASE COUNT          562 a          551 c          614 g          710 t
ORIGIN      
        1 caaacttgac catgatctgc ccgcaacata ctgcaatatg ttcctggttg agggtataga
       61 aagtttgttt gccttcttcg cgcacattga ccaaattggc atcgcgcaat atggcgaggt
      121 gatgcgaaat ggtgggctgt gaaaagccaa ttttctcgac gatctcgctg accgagagtt
      181 cgcaacaaca acactcgctc atgattttct ggcgggtttc gtctgcaatg gctttggcaa
      241 acaggacagg gtcaaatttc atgcggttat tatatagatg tttgtctatt tgtcaagata
      301 aaaaaaactc cgagtcttta agacctcgga gttttgagag ctggttattt acccgttaat
      361 tcgccgagtc tatcaatgta ttcttccagc acggcaaatg catcttcgat gggttgggtg
      421 gaagtcatgt caatgccggc ggttttgtaa acctccatag gggacatgga ggaacccgcc
      481 ttgagaaagt tgatatggtc ttgcgccgcg ccggggactc cgtctagcac gcgtttggca
      541 atggcgtttg cagcggagat tccgatagcg tattggaagg agtaataatc aatataaaga
      601 tgcgtggtga atgttcccca agtgataccg acccgttccc ggtcgaggtg catctctccg
      661 ccaaagcctt cgctgaagag gtctgccatg aggttgttca tataatcggc tgtgagagac
      721 tgtccctttt cggcgcgttg atgcgtttcc aattcgaaac gagcaagagt gggcatgatg
      781 aaaaagtagc ggtggaagtt atccatggct tcttcgagta gagcgatctg gaagtattta
      841 tcttttttcg tcttgagcag gtgggcgcgg gtcattgcct gattaaagtt cgaggcggtc
      901 tctgcaatga tggagggata gagataatac atcatcggct gcgcacggct ggcataccaa
      961 gcatgaatgg agtgacccaa ttcatgcgag agcgtgctca tcgagccgac atcatctgta
     1021 tagctcatca tgatgaaagg atgtgtatca cttgggacgc gagtagagaa tgctccttca
     1081 cgtttgcccg cattgggaga ccagtccacc caacggtctt cgaggcaacc tttgcgcatg
     1141 gtttcaacat attcatcgcc catgggcgcg agaccttcac agatccagtc cacagcggtt
     1201 tcgaatggaa ctttgtgttt ctttgtagtc aacggcgccc agacatcata agggtgaagg
     1261 acttttacac ctaatgcctt gcggcgaatg cgccaatact tgtgccacat gggtagtttt
     1321 ttcttaaaaa tatccaatag gttatggaac atttcgaccg gaacatcgcc attgaaaagt
     1381 gttgcttcca gtgaagagtt gaaattccgt gctttcatgt tgaatacgtt ttgtttgatc
     1441 gaagcagcca gattccctgc cagagtgttc ttgaattcaa gatatttatc gttgtaattt
     1501 tcccaagaag tttggcgcac tttacggtct gctgactcaa tgagagcact tcttgtgctt
     1561 tgagtcaatt cgagctttct gcctttgctg tctttggcgg gcttgaattt gaaatcggca
     1621 tttgccaaca tcccggctgt attgcggact gccccgaacg ggtcgcgtac catgccaagc
     1681 aattcttcca cttcggcatt acgcacatgc gcttgcttgc ggaataagtc attgaggaag
     1741 tgctcgtaca gtttcatgcg cgagtcttct ttcatccatt gacgtaattt cgcctcaccc
     1801 atggacagca attccggctc cacaaatgat gtcgctgccc caacttgtgc cagcatggac
     1861 atggctttgc tgctcatcgc agcgccttgc tcgtcgtttg cgtccacggc gctcgccatg
     1921 gtggcataca cgcgcacctt tgcagtgcgc ttttcgagct tatctatcgc ttgaaacgct
     1981 tcaagaaaca tttcgatgtt ttcaccaaga tgcccttggt attttttgat ttttggaagg
     2041 tcagctagaa tattttgcaa ttcagtatcg aaggcttcag gcgttttaaa gacactttct
     2101 gcattccaag tttggttttt ctttactttt gagcggggta gtggtgtagt catgatacag
     2161 ttcctttcaa tgtgcgctga aagataaata aaaatgtact ccgtattttt agctatgttg
     2221 ccaaaatatc ggagtacatt ttgtcatctt cctgaaacga tagacttact gcttggttag
     2281 cgtggttgga acactttgcg atgtgggagc gcccgtccca ccggcgctgc tgctgaattg
     2341 gatgttcaca gcgcccaaat ctgcgtcggt gatcgtatag ttatttgtac actgcacggt
     2401 tgcattgggt gccagtgtgg tattggctgc accgcaa
//
LOCUS       DAOQFT010000389         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27843_length_2436_cov_6.815624, whole genome shotgun sequence.
ACCESSION   DAOQFT010000389 DAOQFT010000000
VERSION     DAOQFT010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27843_length_2436_cov_6.815624"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLR93_09800"
     CDS             complement(<1..414)
                     /locus_tag="PLR93_09800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27303.1"
                     /translation="MNTSSRNLTSILLTAICFFAFFIFGTTDNLKGPTLPAMLAELNI
                     DYGTGGNIFFSEYVGFLIATLITGILADRFGLKIVFILAGIALVFGVGGYSSFSTPFL
                     LSTSLFIVGLGLGAFELGPNAVIVSLYREQKGLYLN"
     gene            complement(487..1692)
                     /gene="gguB"
                     /locus_tag="PLR93_09805"
     CDS             complement(487..1692)
                     /gene="gguB"
                     /locus_tag="PLR93_09805"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNH27304.1"
                     /translation="MNFFSTARKLLQQNIREYGMYIALFVIMVYFTIMTEGTFISSRN
                     IVNLVNQTGYIAVLAIGMTLVIIIRHIDLSVGFLAGFLGAIAAIAMADPKVVAAGMSL
                     PVYIVVPMTLGLGIVAGLITAFLVAQMKIPSFVASLTGWLIYRGAILVVTESTGTIII
                     NNEAFNAIGNGFIPDIPDIGILPGVHKLTLLLGIFAIVAMIFSELNERRKKQSYNFET
                     LPTEMFVIKLVFISGIIGTITWILAGYQGLSWTAVVVLVVTLVYHFIMSQTVLGRHIY
                     AVGGNPDAAELSGISVKRLVYIVFGSMGMLSALSGILFASRLQSATTTAGNLFELDAI
                     AAAYVGGVSAAGGVGKVLGSIIGALVMTSLTSGMNLMGIGISYQYMVRGAVLAAAVIF
                     DVATRNTGK"
     gene            complement(1705..>2436)
                     /locus_tag="PLR93_09810"
     CDS             complement(1705..>2436)
                     /locus_tag="PLR93_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH27305.1"
                     /translation="EVRNWNARNPALGRDILKDVNIKVNRGEIVGLAGLIGSGRTELA
                     LSIFGNPAKYQVQGDLYVQGEKRSFSHPEEAIRAGLSYVSEDRKGNGLILIQDVKQNI
                     TLANLKELTTRLVVNNNAEIQVANKYKTDLNIKTPTVEQKVSNLSGGNQQKVSLGKWL
                     FAKPNILILDEPTRGIDVGAKYEIYTIMRDLVQKGLSIVMISSELPEVLGMSDRIYIV
                     ASGKIAGELSAQEATQEKIMHLATN"
BASE COUNT          681 a          561 c          600 g          594 t
ORIGIN      
        1 attaaggtat aagccttttt gttcacgata caaactgaca ataacggcgt tgggaccaag
       61 ttcaaacgct cccagcccaa gcccaacgat aaagagagag gttgataaaa ggaacggtgt
      121 actgaatgag ctgtatccgc caactccgaa aacgagagct atccccgcaa gaatgaaaac
      181 aattttgaga ccaaagcggt ctgccagtat gccggtaatg agtgtggcaa ttaagaagcc
      241 gacatattca ctaaaaaaga tattcccacc tgtgccatag tcaatgttta gctctgctaa
      301 catggctggc aatgtaggcc ctttgaggtt atcggtggtg ccaaagataa aaaaagcaaa
      361 gaaacagatg gcagtgagga gaattgaagt aaggtttcgg gaagatgtat tcatttggtg
      421 attatagaac aaaaagagac agggcttgta cgtaagccct gtctctcatt ttgagtgaag
      481 cgaaaattat ttgccggtat tgcgggtggc tacgtcaaag attacggcgg cggcgagcac
      541 tgctccgcga accatgtatt gatatgagat accaatgccc atcagattca tgccgctggt
      601 gagcgaggtc atcacaagcg caccaatgat ggagccaagc actttaccga caccgcctgc
      661 agcagagaca ccacccacgt acgccgcggc aatggcatcc aactcgaaga gattgcctgc
      721 ggtggtggtg gcagattgaa ggcgagaggc aaacaaaatt ccagaaagag ccgagagcat
      781 gcccatcgag ccgaagacga tatagaccag tcgcttcacg ctgatgccgc tcaactcggc
      841 ggcatcgggg ttaccgccga ctgcataaat gtgcctgccg agcacagttt gcgacataat
      901 gaaatgatag acaagggtga caacgagtac aactacggct gtccatgaga gaccttgata
      961 cccagccaaa atccaagtga tggttccgat aatgcccgaa ataaagacca gcttgatgac
     1021 aaacatttcg gtaggcaagg tttcaaagtt gtaggactgt ttctttctgc gttcattaag
     1081 ctcgctgaag atcatggcaa caatcgcaaa aataccgaga agtaatgtga gcttatgaac
     1141 cccagggaga atgccaatat ctggaatatc tgggataaag ccattaccaa tggcattgaa
     1201 ggcttcatta ttgatgatga tagtaccggt gctttctgta actaccaaaa tggcaccacg
     1261 atagatcaac caacctgtta gcgaagcgac gaaagaaggg atcttcattt gagcgacaag
     1321 gaaggcggtg atcaacccgg cgacaatgcc caagccaagt gtcattggca caacgatgta
     1381 cacaggcagg ctcattcctg cggcaacaac tttagggtct gccatggcga ttgcggcaat
     1441 ggcaccgaga aaaccggcta gaaagcctac tgatagatcg atatggcgaa tgataatgac
     1501 caaggtcatg ccaatggcga gcacggcaat gtaacctgtt tggttaacca agttgacgat
     1561 gttgcgcgat gaaatgaacg tgccttctgt catgatggtg aagtaaacca tgatcacgaa
     1621 aagcgcaatg tacatgccat attcgcggat gttttgttgc aaaagttttc gtgcggtact
     1681 gaagaaattc ataagggtat tctcctagtt cgttgccaaa tgcatgatct tttcttgcgt
     1741 ggcttcctga gcagagagtt cacctgctat ttttccagaa gcgacaatgt aaattctgtc
     1801 actcatgcct aacacttcag gaagttcaga agaaatcatg acaatgctca gccctttttg
     1861 gacgagatca cgcatgatgg tatagatctc gtactttgca cccacatcaa tgccgcgtgt
     1921 gggttcatca aggatgagga tattcggctt ggcgaacagc catttgccta ggcttacttt
     1981 ttgttgattc ccgccgctca gatttgaaac tttctgctca acagtggggg tcttaatgtt
     2041 caggtctgtt ttgtatttat tggcaacctg aatttctgca ttgttgttaa caacgaggcg
     2101 agtcgttagt tccttgagat ttgccagtgt gatattttgc ttcacatctt ggatgaggat
     2161 caagccgttg ccttttcggt cctctgagac atacgacaag cccgcgcgga tcgcttcttc
     2221 ggggtggctg aaactgcgct tctcaccttg gacgtacaaa tcgccttgca cctgatactt
     2281 tgccgggttg ccaaagatgc tcagcgccaa ctccgtgcgc ccagacccga tcagccctgc
     2341 cagcccaacg atctcgccgc gatttacttt gatgtttaca tctttcagaa tatccctgcc
     2401 aagtgcaggg tttcgtgcat tccagtttcg aacttc
//
LOCUS       DAOQFT010000390         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_27914_length_2432_cov_6.109382, whole genome shotgun sequence.
ACCESSION   DAOQFT010000390 DAOQFT010000000
VERSION     DAOQFT010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27914_length_2432_cov_6.109382"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLR93_09815"
     CDS             complement(<1..906)
                     /locus_tag="PLR93_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27306.1"
                     /translation="MKFQITLAARYLSGRKLRTFLTTLAVVFGVLVIFGMNIIMPTML
                     SALQVNVQSAEGKVDYTVTHISGEPFSETVAENLAAVDGVRVYAASLNRTINIPADFF
                     DKDTSKLDTITAISLVGINPEAERSLRAFSLVDGRYLQADDSNSALVSRTFADSLHLE
                     VGDTFLLPSADGTTELTVVGILPAQLTSGNETIWVNLPVAQTIMNALGKVNTISINTE
                     TTAMEERRAEVQTNIETTLGDQYHVGSLLSGTEMFASLELAQTMFNVFGVLALFMGGF
                     IIFNTFRTIIAERRRDIGLLRALGAN"
     gene            complement(908..1651)
                     /locus_tag="PLR93_09820"
     CDS             complement(908..1651)
                     /locus_tag="PLR93_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27307.1"
                     /translation="MSNIQIEKLTKTYGSGITQVTALDHVNLTIREGEFVAIMGPSGC
                     GKSTLLHLLGGLDKPSEGSVLIGGHNLTDLNDDKLTELRRRKIGFVFQFFNLIPVLNA
                     TENAALPVTLDGLKPADAQKKAVEWLTRFGLADRLNNRPDQLSGGQQQRVAIARALVA
                     DPELILADEPTGNLDTRSSDEIASLLRDVSKKYNRTVVMVTHDPRIAAYADRIIFLKD
                     GKVVDETLLERKNGKSADYVAGKVKELGD"
     gene            complement(1710..2327)
                     /locus_tag="PLR93_09825"
     CDS             complement(1710..2327)
                     /locus_tag="PLR93_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNH27308.1"
                     /translation="MSVRNAILGLLAQKSRHGYELHSAFSTVMDGATWDVKPAQIYTT
                     LERLQESGLVQTKSDLGEGREPDRRIYAITRDGHEALKSWFNESVPTEHQRDEFYVKL
                     MIGLISGEADPARIIQTQRSRLFQEMHDATTQRDKYDPRLEMAKILLSDKAIMHLEAD
                     LRWLDMIEMRLETIKDQPMPEPEIRKRGRPKKIDAGSTYPPPDEK"
BASE COUNT          601 a          603 c          620 g          608 t
ORIGIN      
        1 attggcaccc aatgcacgca acaaaccaat gtcgcggcgg cgttcggcga tgatagtgcg
       61 aaaggtgtta aagatgataa acccgcccat aaagagagcc aacacgccaa agacattaaa
      121 catagtctga gcgagttcaa gtgaagcgaa catctctgta ccagagagta aagacccaac
      181 gtggtactga tctcccaatg ttgtttcaat atttgtttgc acttcagcac ggcgttcttc
      241 catggcagtc gtttcggtat tgatgctgat ggtgttgacc ttgcccaaag cattcatgat
      301 cgtttgcgct acaggtaggt tgacccaaat cgtctcattg ccagaggtta gctgtgcagg
      361 cagaatgcca accacagtca actctgtcgt gccgtcggcg ctcgggagca ggaaggtatc
      421 tcctacttcc aagtgaagag agtcggcaaa tgtgcgcgag accaaagcag aattgctgtc
      481 atcagcttgc aagtagcgac cgtccaccaa agaaaaagct cgcagggagc gttcggcttc
      541 ggggttgatg cccacaagac tgatcgctgt tatggtatcg agcttgctcg tatctttgtc
      601 gaaaaaatcg gcagggatgt tgatggtacg gttcaatgaa gcagcataaa cacgcacacc
      661 atccacagct gcaaggttct ctgccacagt ttcagaaaag ggttcacccg agatgtgggt
      721 gacggtgtaa tccaccttcc cttcggcact ttgcacattg acctgtaagg cactgagcat
      781 ggtcggcatg atgatgttca tgccgaagat gaccagcacc ccaaagacca cagcaagggt
      841 ggtgaggaag gttcgtaatt tacgtccgct caggtagcgg gcggcgagag tgatctggaa
      901 cttcatttca atcacccaat tctttcacct tgcctgccac ataatcagca gactttccgt
      961 tttttcgttc gagcaaggtt tcatccacca ccttgccatc cttgaggaag atgatgcggt
     1021 cggcataagc ggcgatacgc ggatcatgag tcaccatgac cacggtgcgg ttatattttt
     1081 tcgaaacatc gcgcagtaaa ctggcgatct catcgctgga acgagtgtcg aggttgccgg
     1141 tgggttcatc ggcgaggatc agttccgggt cagcgaccag ggcacgggca atcgcgacgc
     1201 gttgttgttg tccgccagag agttggtcgg ggcggttgtt caaacgatct gccaagccga
     1261 aacgggtcag ccattccaca gctttctttt gcgcgtcagc aggcttgagc ccatccaaag
     1321 tgacaggcaa cgcagcgttc tctgtcgcgt tcaatactgg gatcaggttg aagaattgga
     1381 agacaaagcc gatcttgcgg cgacgcagct cggtgagttt gtcgtcgttg aggtcggtca
     1441 ggttgtgccc gccgatcaac acactgcctt cactgggctt atccaagccg ccgagcaaat
     1501 gcaacagagt cgatttgccg cagccgctgg gtcccataat ggcgacaaat tcgccttcgc
     1561 gaatggtgag gttaacatgg tcaagcgcgg tcacttgcgt gatgcctgag ccataagttt
     1621 tggtaagttt ttcgatctga atattggaca tttttttctt tctctttata aaagcaatgc
     1681 gaacgaataa aacttgcaac tactatgact tatttctcat ctggtggcgg atacgtagaa
     1741 cctgcatcaa tctttttagg tctgccgcgc ttgcgaattt caggctcagg catgggctga
     1801 tctttgatgg tctccaaacg catttcgatc atatcgagcc agcgtaagtc cgcttcgagg
     1861 tgcataatgg ctttatcact caaaaggatc tttgccatct ccaagcgcgg atcatattta
     1921 tcgcgctgcg tcgtggcgtc atgcatttcc tgaaaaagcc gtgaacgctg ggtttggata
     1981 atgcgcgctg ggtcagcttc accagagatc aacccgatca tcagtttgac gtaaaactcg
     2041 tcacgctggt gttcggtcgg cacactttcg ttgaaccacg atttcagcgc ttcatgtcca
     2101 tcgcgagtga ttgcataaat gcgccgatct ggctcccgcc cttcccccag atccgacttg
     2161 gtctgtacca agccagattc ttgtaaacgt tcaagggtgg tgtagatctg ggcaggcttc
     2221 acatcccaag tagccccatc cattaccgtg gaaaaggcag aatgcagttc atacccatgc
     2281 cgggattttt gcgcaagcag acctaaaatg gcatttcgaa cggacaaaag agcctcactt
     2341 gaaaatagta tacgagttac taattacaaa acttgaaaat attatacgag ttactatttt
     2401 ttgtcaagag gaataaaatc acttccgcct ct
//
LOCUS       DAOQFT010000391         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28129_length_2421_cov_8.348267, whole genome shotgun sequence.
ACCESSION   DAOQFT010000391 DAOQFT010000000
VERSION     DAOQFT010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28129_length_2421_cov_8.348267"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1933)
                     /locus_tag="PLR93_09830"
     CDS             complement(134..1933)
                     /locus_tag="PLR93_09830"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HNH27309.1"
                     /translation="MQRHSDFTPIHTILVPYIHDGPGLHALDAARQFDAEIILVGVVV
                     VPEGQSLSTGAAAARAVRRTLRIYGKDKRITSKTQVIVSHKPWEELSNLIQDENPDLL
                     CLEFDSHLKALHVTASDVLSRPPCDVALVRGKISSKPKQVLVPVRGGPHAELAVRVGL
                     GLHPKGVTSLHIRRPEDPEAGTDAPFKPLERVLYQIPEVQKKFEVTEDAAQVILNYAK
                     KSDVVILGTTIQPITSSASLGAVADRLLHEASCAVIAVKSGRPLPHVKFDETLSFDAI
                     SILVDKWFGENTFHADEFSNLREMVALKKQQGVTISLALPALNEEETVGKVIRTMKKE
                     LMQRFPLLDEIVLIDSNSTDRTREIAKKEGVPVYIHQELLPDMEPRRGKGEALWKSLL
                     VTKGDIIVWIDTDIVNIHPRFVYGIIGPLLINPTIQLVKGFYRRPLRVGQKMQAGGGG
                     RVTELTARPLLNLFYPELSGVVQPLSGEYGGRRKALEQVTFYSGYGVETGLLIDIFEK
                     FHLESIAQVDLLERIHHNQHLEALSKMSFAIIQTVIRKLEKRFGRAVLEDVNRSMKMI
                     RHDSKGYFLDVEQIAEYERPPMIEVLEYLNRKR"
     gene            complement(1975..>2421)
                     /locus_tag="PLR93_09835"
     CDS             complement(1975..>2421)
                     /locus_tag="PLR93_09835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH27310.1"
                     /translation="GPLGPHNANNIKYFETLTELRKQLGLEGSVHFLAELHNGFLPDE
                     VIADFYRVADALFFPSREEGFGIPLIEAAFSHLPAFCADIPPLRKLGLNDTQFFSPDE
                     DPARISKILTDYFQTSMPARLAMRARASFRWEAIYRQHIEPLLVRA"
BASE COUNT          587 a          578 c          616 g          640 t
ORIGIN      
        1 gcaagttgaa gaaatagagt catggttcac cttgagattt tgtataccat gaactttttt
       61 cgttaagatt aattccactt taaacaaaaa gagacagtca tgtgcttgat gactgtctct
      121 tgctttgaat cgtttatctc ttcctattta aatattccag cacctcgatc atgggcggac
      181 gttcgtattc ggcgatctgt tccacgtcga ggaagtatcc cttgctatcg tggcggatca
      241 ttttcatcga gcggttcaca tcttccaaca cggcacgacc gaagcgcttc tccaatttgc
      301 gtatgacggt ttggatgatg gcgaaggaca ttttgctcaa cgcctctaaa tgctggttgt
      361 gatggatgcg ttcgagcagg tccacttggg caatcgattc caaatggaat ttctcaaaga
      421 tgtcgatcaa caaaccggtt tccacaccgt agcctgaata gaaggtgact tgttccaatg
      481 ctttgcgtct gccgccatat tcgcccgaaa gcggttggac gacaccagag agttccgggt
      541 aaaacaaatt cagcaatggg cgtgcggtca attcggtcac tcgtccgccg ccgcctgctt
      601 gcatcttttg ccccactctc aacggtctac gataaaagcc tttgaccagt tggatggttg
      661 gattaatcaa caacggaccg ataatgccat acacaaaacg cggatgaatg ttgacgatat
      721 ctgtatcgat ccatacgatg atgtcacctt tggtgactag taaacttttc cacagggctt
      781 cgcctttgcc acggcgcggt tccatatcgg gcaataactc ttgatgaatg tatacaggta
      841 cgccttcttt ttttgcgatc tcacgtgtac ggtcggttga attggagtcg atcaacacga
      901 tctcgtccaa caatggaaag cgctgcatca attctttttt catggtgcga atcactttgc
      961 caaccgtttc ttcttcgttc aaggctggca atgccaaact gattgtgacc ccttgttgtt
     1021 tctttagcgc aaccatctcg cgcaaattgc tgaactcatc agcatgaaaa gtattctcgc
     1081 caaaccattt gtcgacaaga atcgaaatgg catcgaagct caaggtttca tcgaacttga
     1141 cgtgcggcaa gggacgtccg cttttgacgg cgatgacagc acaagatgct tcatgcaaga
     1201 ggcggtctgc cacagcgccg agagaagccg aactggtgat gggttgaatg gtggttccca
     1261 aaatgaccac gtctgatttt ttggcgtaat tcaaaatgac ttgcgccgca tcttctgtga
     1321 cctcgaactt tttttgcact tcagggattt gatacaacac gcgctccaac ggtttgaatg
     1381 gagcatcggt tccggcttcg gggtcttcgg gtctgcggat gtgcagggat gtgacgcctt
     1441 tgggatgcaa tcccaagccc acgcgcactg ccaactccgc atgcggaccg cctcttactg
     1501 gaaccagaac ctgtttcggt ttactggaaa tttttcctcg taccaaagcc acgtcgcagg
     1561 gcggacggga gagcacgtcg ctggcagtga cgtgtaaagc cttgagatgt gaatcaaact
     1621 caagacaaag cagatccggg ttttcatcct gaatcagatt tgaaagttct tcccaaggtt
     1681 tatgtgaaac gatcacttgt gtcttgctgg tgatgcgttt gtctttgccg taaatgcgca
     1741 aggtgcggcg taccgcgcgt gccgctgccg cgcccgtgct aagggactgc ccctcaggca
     1801 caacgaccac accaaccaaa atgatctcgg catcgaattg acgggcagca tcaagggcat
     1861 gtagaccggg tccgtcatga atatagggaa caaggatcgt gtgaatgggc gtgaagtcgg
     1921 aatgtctttg cattgttgtc ctttgtcttg aagacctaaa ggttttttaa acctttaggc
     1981 tctgactaat aacggctcga tgtgttgacg atagatcgct tcccaacgaa acgatgcccg
     2041 tgcccgcatg gcaagccgcg caggcatgga cgtttgaaaa tagtctgtga gtatttttga
     2101 aatacgagca gggtcttcat cgggtgagaa aaattgagta tcgttcaagc ctaattttcg
     2161 gagcggcggg atatccgcgc agaaagcggg caggtgactg aaggctgctt cgatgagcgg
     2221 aatgccaaag ccttcttcgc ggctggggaa gaagagcgca tctgccactc gataaaaatc
     2281 ggcgatgact tcatcgggta aaaaaccatt gtgtagttcg gcgaggaaat gaacagaacc
     2341 ttccaagccc aattgcttgc gcagttcggt gagtgtttca aagtatttga tgttgttcgc
     2401 gttgtgcggt ccgagcggac c
//
LOCUS       DAOQFT010000392         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28215_length_2417_cov_8.783235, whole genome shotgun sequence.
ACCESSION   DAOQFT010000392 DAOQFT010000000
VERSION     DAOQFT010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28215_length_2417_cov_8.783235"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLR93_09840"
     CDS             complement(<1..1154)
                     /locus_tag="PLR93_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27311.1"
                     /translation="MSKEHSRYRWFVVAVFFFFMLLHQTDKLMIGSLQVQISNDFNIN
                     NKQWGLVNSGALIVATILYPIWGYLYDRYSRTKLLSLASFIWGSTTWLNAIMKSFGGF
                     LVTRASTGIDDSSYPGLYTLIADYFGPTQRGKIFGILQLAQPIGYLLGMILALMVAPA
                     IGGWRSVFYITGSLGLVIALLIFFGVREMPRGKAEPEFENMTEMQTFKFSWAEAREIF
                     KKKTMWFVFLQGFAGVFPWNVITFFFFAYLEKERGYDANSILFTMAPVILILASGYFV
                     GGALGDYAFKRSNKGRIIVSSVGVLLGALFMYLAITTPLEAKNQFFVFMCLTAIFMPL
                     SSTNVTATVYDVTVPEVRSTAQAVEYFIENAGAAFAPIITGVIADMYDLKT"
     gene            complement(1207..>2417)
                     /locus_tag="PLR93_09845"
     CDS             complement(1207..>2417)
                     /locus_tag="PLR93_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNH27312.1"
                     /translation="FADAHIDMANYGRHDPSTGLPLRVLDFLKSLDTIVDTAIERKVD
                     MVIFAGDAYKDRSPAPTFQREWGKRIMRLSQAKIPTLLLIGNHDISPSIGRAHALQEF
                     KTLQVPYVKVLDAPELLKPADLWDLPLQVIAMPWVARSGFMAATGETDSTQAFSRIEE
                     NISELITGWIEECDPSMPIVLTAHASIEGAKFGGERLVMLGNDLVLTGGLVKNPKLNY
                     VAMGHIHKPQDVNEGHQPPVIYPGSIERVDFGEAKEERFFVIADVEKGRNTEVEWIQL
                     TGVRKFIDRRATIESSESVTEFLKSKLPKPKEMSEAIIRLIVEYPREWDSLIDESALR
                     KYAADAFEFHFVKRPQIESRVRIDEGQVVSSLSPLDLLDQYFDAAKVSNADELKNLAQ
                     EIISEDSQDS"
BASE COUNT          686 a          626 c          535 g          570 t
ORIGIN      
        1 gtcttaaggt catacatatc ggcaatcacg ccggtaatga tgggagcaaa cgcagccccg
       61 gcattttcaa taaagtattc aaccgcctgc gccgttgacc gcacttcagg gaccgtcaca
      121 tcatacactg ttgccgtcac attggtagaa gacaacggca taaagatggc ggtaaggcac
      181 ataaagacaa aaaattgatt cttggcttct aatggtgtgg tgattgcaag gtacatgaac
      241 aacgctccaa gcaacacacc aacagaggag acaataatac gtcccttatt tgaacgcttg
      301 aaagcataat cacccaaagc accgcccaca aagtatccgc tggcaagaat caagatgaca
      361 ggcgccatgg taaataaaat gctattggca tcgtaaccgc gttctttttc aagatatgca
      421 aagaagaaaa acgtaatcac attccatggg aacacaccag caaagccctg caagaaaaca
      481 aaccacatgg tcttcttctt gaagatttct cgcgcttctg cccatgagaa cttgaaggtt
      541 tgcatttccg tcatattctc aaattcaggt tcggctttac cccgcggcat ttcacgcacg
      601 ccaaagaaga tcaataaggc aatcaccaat cccaacgagc ctgtgatata gaaaacagat
      661 cgccacccac caatcgcagg agccaccatc aaggcaagga tcatacccag taaatagcca
      721 atgggttgag ccaactgcaa aataccgaag atctttccgc gttgagtggg tccgaaataa
      781 tcagcgatta gggtatacaa gcccgggtat gatgagtcat caatgccggt ggaggcacgc
      841 gtaacaagga atccgccaaa gctcttcatg atggcgttca accatgtagt agaaccccaa
      901 ataaaagatg ctaacgaaag cagttttgtg cgtgagtatc ggtcgtacaa ataaccccag
      961 atcgggtaca agatcgtggc aacgatcaat gccccagaat taaccaaccc ccattgtttg
     1021 ttgttgatat tgaaatcatt gctgatctgc acctgtagtg aaccgatcat taacttatcg
     1081 gtctggtgca acagcatgaa aaagaagaac acagcgacca caaaccagcg ataccgtgaa
     1141 tgttccttgg acataaaaac ctcatgacgt tgggatgccc aagtatcagg tgtccgtgtg
     1201 gaagtgtcaa gagtcttgtg agtcttctga aatgatttcc tgcgccaggt ttttcaattc
     1261 atctgcattc gaaacttttg ccgcgtcaaa atattggtca agcaaatcca gtgggctaag
     1321 gctgctcacc acctgccctt cgtcaatgcg cactcgcgac tcaatttgag gtcgcttcac
     1381 aaagtgaaac tcaaacgcat cggcggcata ttttcgcaac gctgattcgt cgatcaacga
     1441 atcccattca cgtggatatt caacgatcaa gcggatgatc gcctcagaca tttccttagg
     1501 cttaggcaat ttgctcttga ggaactcggt cacgctctca ctcgactcga tagttgcccg
     1561 gcggtctata aacttcctca cccctgtgag ttgaatccac tctacttcgg tgttgcgtcc
     1621 tttttccaca tctgcaatga caaagaagcg ctcttctttc gcctccccaa agtcgacacg
     1681 ttcaatggag ccggggtaaa tcacgggcgg ttgatgtcct tcgttcacat cttgcggctt
     1741 gtggatatga cccattgcca catagttgag cttcgggttt ttcaccaatc cgccagtcaa
     1801 caccaggtca ttgccaagca ttaccaagcg ttctccgcca aactttgcgc cttcaatgga
     1861 agcatgtgcc gtcaacacaa ttggcatgga ggggtcacat tcctcgatcc aaccggtgat
     1921 tagctcacta atattttctt caatgcgtga aaacgcctgc gtggaatccg tctcaccagt
     1981 ggctgccata aacccagagc gtgccaccca cggcatggca ataacttgca atggcaaatc
     2041 ccatagatcg gctggcttta ataactcagg agcatccaac acttttacat aaggcacttg
     2101 cagggtttta aactcttgca aggcgtgagc ccgcccaatg gacggggaaa tgtcgtgatt
     2161 gccgattagt aggagagtcg gaatcttcgc ttgagataaa cgcatgatgc gtttgcccca
     2221 ttcccgttgg aaggtcggag ccggagaacg atccttatat gcatcgccag caaaaatgac
     2281 catatccacc ttgcgttcga tggcggtatc cacaatggta tccaacgatt tgagaaaatc
     2341 caacacacgc aacggcaacc ctgtagacgg gtcatgccgc ccatagttcg ccatgtcaat
     2401 gtgagcatcg gcaaaat
//
LOCUS       DAOQFT010000393         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28266_length_2414_cov_7.629080, whole genome shotgun sequence.
ACCESSION   DAOQFT010000393 DAOQFT010000000
VERSION     DAOQFT010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28266_length_2414_cov_7.629080"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLR93_09850"
     CDS             complement(<1..828)
                     /locus_tag="PLR93_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27313.1"
                     /translation="MATAISVKNIGKQYKIGAAETKFRYNMLRDVIVDTVAAPVRLAK
                     AMIGKSDRRLNQNYVWALKDVSFDLEEGKVLGIVGRNGAGKSTLLKILSRVTDPTTGS
                     VTVRGRVGSLLEVGTGFHPELTGRENIYMNGAILGMKRSEIESKFDEIVDFSEVSQFI
                     DTPVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQKKCLGKMGDVAQQGRT
                     VLFVSHNMSAILRLTQEAVVLNKGQLIMRGPTQEAVDYYLSSGQSQAGERTWDADEVP
                     "
     gene            complement(828..1673)
                     /locus_tag="PLR93_09855"
     CDS             complement(828..1673)
                     /locus_tag="PLR93_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27314.1"
                     /translation="MTELTKHEPHTVYIKPSKGLAALNLRDLWVYRELVYFMVWRDVK
                     VKYKQTLLGMAWAVVQPVMTMLVFTFLFGKVAKLPTDGIPYPVFSFTALLPWGLFVNA
                     LNQGSRSLVAHNNMVTKIYFPRLILPMASVFSGMVDFVIAFVILVGLMFYYQVTPAWN
                     LLWTLPLFLLLALVTALGVALWLSAINVQYRDVNQALPFLSQFWLFATPVAYSSKVIS
                     EKWQVVYSLNPMAGVVNGFRWALLGTGNGPDISLWVSVGISILVFVTGLFYFKSMEKT
                     FADTI"
     gene            complement(1676..>2414)
                     /locus_tag="PLR93_09860"
     CDS             complement(1676..>2414)
                     /locus_tag="PLR93_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNH27315.1"
                     /translation="GIGANREHPAEFFYDNMIMGVELMHQAYKHGVGKFVATGTVCAY
                     PKFTPVPFKEDAIWDGYPEETNAPYGLAKKMMLVQAQTYRQQYGFNAIYLLPVNLYGP
                     RDNFNLQTSHVIPALIRKAVEAGERGDKELPAWGDGSPTREFLYVEDAADGIVTAAEN
                     YNGDLPVNLGSGYEISIKDLTEMVAKLTDFQGKIVWQTDKPNGQPRRGLDVSRAKELF
                     NWSAQVSFEEGMKRTIEWFKANRDKIK"
BASE COUNT          668 a          621 c          564 g          561 t
ORIGIN      
        1 tggaacctca tcggcatccc aagtgcgttc tccggcttga gattgtcccg aagagaggta
       61 gtaatctacc gcctcttgag taggtccacg catgataagt tgacccttgt tcaacacaac
      121 cgcttcttgc gtaaggcgca aaatcgcaga catattatgg ctgacgaaca aaacagtgcg
      181 tccctgctga gcaacatcac ccatctttcc aagacatttc ttttggaatt cggcatcgcc
      241 tacagcaagc acttcatcca ccacaagaat ttcaggttcg agatgcgccg ctacggcaaa
      301 tgccaaacgc aagtacatac ccgatgaata tcgcttcaca ggtgtatcga tgaactggct
      361 cacttcagag aaatccacga tctcatcgaa ctttgattcg atctcagagc gtttcatacc
      421 gaggatggcg ccgttcatgt agatattctc acgcccggtc aattcagggt gaaagcccgt
      481 tcctacctca agcagggagc caacccgtcc gcgcacggtg acagaaccag tggtcggatc
      541 agtgacgcgg gaaaggattt tcagcaaggt agatttacca gcaccgttac gccccacaat
      601 gccaagcact ttgccttctt cgaggtcaaa ggaaacatct ttcaatgccc agacataatt
      661 ctggttcagg cggcggtcag actttccgat catagccttc gccaaacgca cgggcgctgc
      721 aaccgtatcc acgatcacat cacgcaacat gttatagcgg aatttggttt ccgccgcgcc
      781 gatcttgtat tgcttaccaa tattttttac tgaaatcgct gttgccatta gatcgtatcc
      841 gcaaaggtct tttccatact tttgaagtag aagagacccg tcacaaaaac caaaattgaa
      901 attccaacag aaacccacaa cgagatatcc ggtccattgc ctgtaccgag caatgcccaa
      961 cggaaaccgt tgaccacacc cgccatcggg tttagggaat agacaacctg ccacttctct
     1021 gaaatgacct tggaggaata tgccactggt gtagcaaaca accagaactg cgaaaggaag
     1081 ggaagcgcct gattcacgtc acgatattga acgttaatag cagaaagcca aagcgccact
     1141 ccaagtgcgg tcacaagagc aagcaagagg aagagtggaa gcgtccagag taaattccag
     1201 gcaggggtaa cttgatagta gaacatcaag ccaaccaaga tgacaaaggc aatcacaaaa
     1261 tccaccatgc ccgaaaagac agaagccatc ggcagaatca gacgcggaaa ataaatcttc
     1321 gtcaccatgt tattgtgcgc cacaagcgaa cggctgcctt ggttaagtgc gttgacgaat
     1381 agtccccacg gcaaaagggc ggtaaacgaa aagaccgggt aaggaatgcc atctgtaggt
     1441 aatttagcga cttttccaaa gaggaaagtg aagaccaaca tggtcatcac cggttggaca
     1501 actgcccaag ccataccaag caacgtctgc ttgtatttca ctttgacatc acgccaaacc
     1561 atgaaataaa ccaattcgcg atacacccaa aggtctcgca agttgagtgc cgcaagccct
     1621 ttagacggct tgatataaac agtatgaggt tcgtgttttg taagttcagt catatttact
     1681 tgatcttatc gcgattggct ttgaaccatt cgatggtgcg cttcatgcct tcttcaaatg
     1741 aaacttgtgc gctccagttg aagagttctt ttgcccgcga gacgtcaagt ccgcggcggg
     1801 gctgaccatt tggcttatct gtctgccaga cgattttgcc ttggaagtcg gtgagttttg
     1861 caaccatctc ggtgaggtct ttgatagaaa tttcataccc agaaccaagg ttgaccggca
     1921 aatcgccgtt gtagttttcg gcagctgtca caatgccgtc ggcggcatct tccacataga
     1981 gaaattcacg agtgggtgaa ccatcgcccc aagcggggag ttctttgtca ccacgttcgc
     2041 cagcttccac tgccttgcga ataagcgcag ggatgacatg tgaggtctgc aaattaaaat
     2101 tatcgcgcgg accatagagg ttcaccggca aaagataaat ggcattgaag ccatattgct
     2161 ggcggtaggt ttgagcttgt accagcatca tcttcttggc aagcccgtaa ggagcgttgg
     2221 tctcttcggg ataaccatcc caaatagcat cttctttaaa tggcacaggc gtgaacttgg
     2281 ggtatgcgca taccgtccca gtggcaacaa acttacccac accatgcttg taggcttggt
     2341 gcatgagttc cacacccatg atcatgttgt cgtagaaaaa ttcagccggg tgttcacgat
     2401 tcgcaccgat gccg
//
LOCUS       DAOQFT010000394         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28431_length_2406_cov_7.776691, whole genome shotgun sequence.
ACCESSION   DAOQFT010000394 DAOQFT010000000
VERSION     DAOQFT010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28431_length_2406_cov_7.776691"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLR93_09865"
     CDS             <1..180
                     /locus_tag="PLR93_09865"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNH27316.1"
                     /translation="NGFLAAGLLWGLVAGDPVKIFFLSCVIIAGIFGAFTVSKRIFYI
                     QAVPAIVALVILLVA"
     gene            complement(182..1720)
                     /locus_tag="PLR93_09870"
     CDS             complement(182..1720)
                     /locus_tag="PLR93_09870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH27317.1"
                     /translation="MTPPNILYSTALLTAGICCFIVGLLVLQRRPFMPGSIPLMVFIL
                     AMCWWDISYGIFWGGVLKPSPYFWLDISYIGVVIVPAAFFVFAMQISGRQEWLKTPLL
                     IGLYVEPLLVLVLMWTDPWHNLFFAGKRLENTGMILNAGPVFWANVVYSYLLLLVTFI
                     LLVRRFTTTSGIYRRQLGVVIAGAGVTWLNSFIFVTGLSPFPNADNTPFSFTIAAFAF
                     AFALLRYRMLDIVPVARDTLIEGMSDGVLVLDTNNRIVDMNPEAEKLLSASSAGTIGR
                     HVEEVFAPWADLVKTFMNVDQTRVEVLASRDSDTYLDLQISPLFDKRKNFIGRLVMWR
                     DISRLKKIQTELEKLATQDPLTHVYNRRHFYTLAEVEIHRSLRSQLPFSVILVDIDYF
                     KSINDKYGHKAGDEALIHFTKICIQNLRKVDIFARFGGEEFVILLPEADLEQAKETAE
                     RIRKIVESTVLNIDEHQIKITASFGVSTFKGSPDTLDLLLQKSDEALYAAKNTGRNRV
                     VCAD"
     gene            complement(1799..>2406)
                     /locus_tag="PLR93_09875"
     CDS             complement(1799..>2406)
                     /locus_tag="PLR93_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNH27318.1"
                     /translation="GKTFRHESAVLGGAPVFSVMREAFPLADLESFKGIFNATTNVIL
                     SRMESGETYEQALAYAQKIGLAETDPTNDVDGWDAAIKVASLVTVLWDTPMTPQQVNP
                     TGIRGITSEMIANAKKEGKRYKLVCSAERTDGKIVASVSPQLVDSSSSLYGMMNSSTG
                     ITFRTDVIIDYSIVLSEKPGMTGGPVETAYGLFADFVNIVR"
BASE COUNT          673 a          584 c          558 g          591 t
ORIGIN      
        1 aacggcttcc tcgccgccgg tttgctgtgg ggactcgtgg caggcgaccc ggtgaaaatc
       61 ttcttcctca gttgtgtcat catcgcagga atcttcggcg cattcaccgt cagcaagcgc
      121 atcttttaca ttcaggctgt ccccgctatt gtagccttgg tgattttgtt agttgcataa
      181 gttagtctgc gcagacgacc cgattgcgtc ctgtattttt tgcagcatac aaagcttcat
      241 ctgatttttg cagcaaaagg tctaaggtgt cggggctgcc tttaaaggtg gaaacaccaa
      301 aactggcagt gatcttgatt tgatgttcat caatgttcag cactgtgctt tccacgatct
      361 ttcgaattcg ctcggctgtc tctttggctt gttccaagtc cgcttcgggc agcaagatga
      421 caaattcttc gccgccaaat ctggcaaata tatccacttt gcggaggttc tgtatgcaga
      481 tcttcgtaaa gtggattagt gcttcatcgc cggctttatg tccgtattta tcgttaatgc
      541 tcttgaagta atcaatgtcc accaaaatga cagagaaggg caactgagaa cggagagagc
      601 ggtgaatttc cacttctgcc aaggtgtaaa aatgacgtcg gttgtagacg tgggtcagcg
      661 ggtcttgtgt ggcaagtttt tcaagctcgg tttggatttt cttcaagcgt gaaatatcac
      721 gccacattac caaacgtccg ataaagtttt ttcgtttatc gaacaatggg gaaatttgaa
      781 ggtcaagata tgtatctgag tcacggctgg ctagaacttc cacccgggtt tggtccacgt
      841 tcataaatgt ttttacaagg tcagcccacg gggcgaaaac ttcttctacg tgcctgccaa
      901 tcgtcccggc ggaagaggcg gagagaagtt tttccgcttc gggattcata tccacaatgc
      961 gattgtttgt atccaaaaca agcacaccgt cgctcatacc ttcgatcaat gtatcgcgcg
     1021 ccacgggcac aatatccagc atgcgataac gaagcaaagc aaaggcaaac gcaaaggcag
     1081 cgattgtaaa tgaaaaaggc gtgttatctg cattcgggaa agggcttaaa ccagtgacaa
     1141 aaataaaact attgagccag gtaactcctg cccccgcaat cacaacccca agctggcgac
     1201 gataaattcc agaagtcgtt gtaaatctac gcactaacaa aatgaacgtg accaataaca
     1261 acagataaga atatacaacg tttgcccaaa ataccggtcc tgcatttaag atcatgccgg
     1321 tgttttccaa ccgtttgcca gcaaagaaaa gattatgcca ggggtcggtc cacatcagaa
     1381 caagaaccaa caacggttct acatacaacc caatcaacaa tggtgttttc aaccattctt
     1441 gcctgcctga gatttgcatg gcaaaaacaa aaaacgccgc cgggacgata accaccccga
     1501 tataactaat atccaaccaa aagtaaggtg aaggctttaa aacgccgccc cagaaaatgc
     1561 catacgaaat gtcccaccag cacatggcaa ggatgaaaac catcaatggg atcgaccccg
     1621 gcataaacgg gcgtctttgt aacaccaata acccaacaat aaagcaacaa atcccagccg
     1681 ttaagagtgc agtactatac aagatgtttg gaggggtcat aaaagccttt gtgtctacta
     1741 ttgtaaacac aaaggctcta ctttactact taacaatgat attcgaataa aagggcagtt
     1801 agcggacgat attcacaaaa tcggcaaaga gaccgtatgc ggtttccacg ggtccacccg
     1861 tcataccggg tttttcagag aggacaatgg agtaatcaat aatcacatct gtgcggaagg
     1921 tgatgccagt gctagagttc atcatgccgt acagagacga agatgaatcg accaactgcg
     1981 gtgaaacact ggcaacaatc ttgccatccg tcctttcagc tgagcaaacc aacttatagc
     2041 gtttgccttc tttcttggcg ttggcgatca tctcactggt aatgccacga atgccagtgg
     2101 gattcacttg ctgcggggtc atgggcgtat cccataacac tgtgacaagc gatgcgacct
     2161 tgatggcagc atcccagccg tccacatcgt tggtgggatc ggtttcagcg agaccaatct
     2221 tctgagcata cgcgagcgcc tgttcatagg tctcgccgct ttccatgcga gataagatga
     2281 cgttggtggt agcgttgaag atgcctttga aggattccaa atccgcgaga gggaacgcct
     2341 cacgcatgac cgagaaaacg ggtgcgcctc ccaaaacagc agactcatgc cggaacgttt
     2401 taccct
//
LOCUS       DAOQFT010000395         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28756_length_2389_cov_6.845758, whole genome shotgun sequence.
ACCESSION   DAOQFT010000395 DAOQFT010000000
VERSION     DAOQFT010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28756_length_2389_cov_6.845758"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLR93_09880"
     CDS             <1..501
                     /locus_tag="PLR93_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27319.1"
                     /translation="TSMFGIFMMLSTIPGGWLSDKAGERVGIAAGMFLMSVALFILIV
                     IPQGTQWQWLFFVGWAVAGLGAGISGPAYQSLISKAVPQKLRGLAFGLFSTSLGIVSL
                     PAPWIGAQLWARFGPTAPFKITATVILLSIIPIWLKFKLPKTTEETQPEAEQPVEENK
                     VAIAAG"
     gene            542..928
                     /locus_tag="PLR93_09885"
     CDS             542..928
                     /locus_tag="PLR93_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27320.1"
                     /translation="MANVEERMKILKLIEEGKISAEEGAKLLSALNDSRKNIPTPPRP
                     PSAPGGARMLRVRVTDTRTGRSKASVQIPLALVDAGLKIGAHFAPEVQGVDMSNVMEA
                     LRMGVTGKIIDVTDEEDGEHVEIFVE"
     gene            1080..>2389
                     /locus_tag="PLR93_09890"
     CDS             1080..>2389
                     /locus_tag="PLR93_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27321.1"
                     /translation="MQETKTNRPSGMFAFTIVWLGQIISVLASAMSQFGLTIWMYEKT
                     QSATALGLMQVFFITPFLIISPIAGVMVDRHNRKLMMMVSDLTAGLATILILIFQSLG
                     VLQFWHLYVASIIYGLGNAFQWPAYSAAISTMVPKDQYGRANGMMSLIEAGPGVFAPL
                     LAGALLPLIGLTGILVIDVVTFSFAVGALLIVHIPQPERTVEGVQGKGSVWQEAVFGF
                     KYIFARPSLLGLQMVFFFGNLFSGILFTLLAPMILARTDNNSLMFGTVQTAGAIAGVV
                     GGLIMSAWGGFKRKVHGVLGGWAITGLGSAVIGLSFGLPAWVIGVAIASLISPLVNAS
                     NQAIWQSKVAPDLQGRVFSARRLIAWFTNPISPIVAGTLADYVLEPQMRAGGTLSQTF
                     SWLVGTGPGSGMGLIILACGVFCVLIGVAGYFIPAIYNAETILP"
BASE COUNT          513 a          509 c          663 g          704 t
ORIGIN      
        1 acttcgatgt tcggtatttt tatgatgctg agcaccatcc ccggcggttg gctttccgat
       61 aaggcagggg agcgggtggg gattgcggct ggcatgttct tgatgtctgt agctttgttc
      121 atcttgattg tcatccctca agggacgcag tggcagtggt tattctttgt ggggtgggct
      181 gtggctggct tgggggcagg gatttccggt ccggcgtatc aatctttgat cagtaaagcg
      241 gttccacaaa aattgcgcgg acttgctttc gggctgttta gcaccagttt gggcatcgta
      301 tcgcttccgg ctccgtggat tggggctcag ttatgggctc gttttggtcc gacggctccc
      361 ttcaagatta ctgctactgt tattttgctt tcgatcatcc caatctggtt aaaattcaaa
      421 cttccaaaaa caactgaaga aacccagccc gaagcagagc agccagtaga agaaaataaa
      481 gtcgctatcg cggcaggata aaataagcgt tcgtcctgaa cggcgaaagg agaaatggaa
      541 catggcaaac gtagaagaaa gaatgaaaat tctgaaatta attgaggaag gcaaaatcag
      601 tgcggaggag ggcgctaagt tgctctcagc tctgaacgac tcgcggaaga atatccccac
      661 gcctccgcgc ccgccttctg ctcccggtgg tgcacgcatg ttgagagtcc gcgtgacaga
      721 tacccgcact gggcgttcca aagcctctgt tcaaattcca ctggcgttgg tggatgccgg
      781 gttgaagatt ggtgctcatt ttgctcctga agttcagggt gtagacatga gtaatgttat
      841 ggaagcactc cgcatgggtg ttaccggcaa gatcattgac gtgaccgatg aagaagatgg
      901 tgaacacgtc gagatctttg tcgaataaag tctgtcggtt cgtgttggtt atcagaagga
      961 gaaaactatg ttcccatcac tataccttgt ccatcgcttg atgttatagc catcaacgcg
     1021 caagagattt ctcttgcgcg ttgtatcatt tttatcacag atcatcaaag gatactttta
     1081 tgcaagagac aaagacaaat cgccccagtg gtatgttcgc atttacgatt gtgtggctgg
     1141 ggcagatcat ttccgtgctt gccagtgcga tgagtcaatt tgggcttacg atctggatgt
     1201 acgaaaagac ccaaagcgct actgcgctgg gtttgatgca agtcttcttc attaccccat
     1261 tcttgattat ttctcccata gctggtgtga tggtagaccg gcacaaccgt aagttgatga
     1321 tgatggttag cgacttaacc gctggacttg ctacgatttt gattttaatc tttcagtcgt
     1381 tgggtgtttt gcaattctgg catttgtatg tagcttcgat catttacggg ttgggaaatg
     1441 ctttccaatg gcccgcatat tcggctgcaa tttccacgat ggtgccgaag gatcagtacg
     1501 gtcgcgcaaa cggcatgatg tccctaatcg aggctggacc cggcgttttt gccccgctat
     1561 tagccggggc gttgctgcca ttgatcggtt tgactggcat ccttgttatt gatgttgtga
     1621 cattttcatt tgccgttggt gccttgttga tcgtccacat ccctcaacct gaacgaacgg
     1681 tggaaggtgt tcagggaaaa ggcagtgttt ggcaggaggc ggtttttggt ttcaaatata
     1741 tttttgcccg ccccagcctg cttggtttgc aaatggtgtt tttctttgga aatttatttt
     1801 cgggtattct gttcacttta ttagcgccca tgatccttgc aagaaccgat aacaacagct
     1861 taatgtttgg tacggttcaa acggcaggag caattgctgg tgtagtgggt ggcttgatta
     1921 tgagtgcctg gggcgggttt aaacgcaagg tacatggcgt gttaggtgga tgggcaatta
     1981 cagggcttgg gtctgctgtg attggcttga gttttggtct gcctgcatgg gtgattggcg
     2041 ttgctattgc ttcgttgatt agtccgcttg tgaatgcatc taatcaggcg atttggcaat
     2101 caaaggtggc accagatctt caaggacgtg ttttttccgc acggcgtttg attgcttggt
     2161 tcacaaatcc catttctccc attgttgctg gaacactggc ggattatgtc cttgagccgc
     2221 aaatgcgagc cggtgggacg ctttcgcaga cctttagctg gttggtgggc accggtcccg
     2281 gttcagggat ggggctgatc attttggcat gcggtgtgtt ttgcgtctta atcggcgtag
     2341 cggggtattt catccctgca atttacaatg cagagacgat cttgcctga
//
LOCUS       DAOQFT010000396         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28758_length_2389_cov_5.806769, whole genome shotgun sequence.
ACCESSION   DAOQFT010000396 DAOQFT010000000
VERSION     DAOQFT010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28758_length_2389_cov_5.806769"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLR93_09895"
     CDS             complement(<1..554)
                     /locus_tag="PLR93_09895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNH27322.1"
                     /translation="MTTGLPESKEKRVFNNVLGAMGNTPLVKLERIGKDLPVPLYAKL
                     EFMNPGGSVKDRVGANIIEQAEKRGEIKPGGTIVEATSGNTGVGLAIAAALKGYKTIF
                     VMPDKMSNEKILLLRAYGAKVVITPTAVGPDDPRSYYEVAKKFARETPNAILANQYHN
                     PDNPKTHELSTGPEIWEQTDGKVT"
     gene            complement(684..1094)
                     /locus_tag="PLR93_09900"
     CDS             complement(684..1094)
                     /locus_tag="PLR93_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27323.1"
                     /translation="MKAKKLSILLAAIMLVAVQLACAAGEPTFSNVRTATDQDGTQLS
                     TTFGSFDTVYVVGDLSNGVVGNNVTARLFVDQVDGFDPNFFVGEAAVDVDQDNINSVY
                     FYFEPPTDGWPVGTYKVEVYFNGTLNTTLSYSVQ"
     gene            complement(1191..1910)
                     /locus_tag="PLR93_09905"
     CDS             complement(1191..1910)
                     /locus_tag="PLR93_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNH27324.1"
                     /translation="MTELLYQTDAYLKEFSAVITSVDAEARAVVLDRSAFYPGGGGQP
                     CDFGTLEVNGVKYPVEKVKKQGDDVLHVLGGTEPLPTPGSASLGTLDWVRRHKLMRTH
                     TALHILCGTVFRDYGAKVTGGDMEPLKGRMDFEFETMRAELVREIEAAVNAEVKKNHE
                     IRVKILPREEAFQIPDLIRTKINLLPEGIMQVRTVEIVGLDLQADGGTHVANTSEVGT
                     IKVVEYKSKGAINKRLYIEIE"
     gene            2109..>2389
                     /locus_tag="PLR93_09910"
     CDS             2109..>2389
                     /locus_tag="PLR93_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH27325.1"
                     /translation="MIKVSGLTKDYGSRRAIHNLSFDAQQGEIVGFLGPNGAGKTTTM
                     RILTGYMPPTDGEAIVAGYDVVEESLEVRKRVGYLPESVPLYYDMVVSE"
BASE COUNT          580 a          607 c          564 g          638 t
ORIGIN      
        1 tcggtcactt tgccatcggt ctgctcccaa atttcgggcc cggtgctcag ttcgtgagtc
       61 tttgggttat cggggttgtg atattgattc gctaaaattg cattgggggt ttcgcgggca
      121 aacttctttg ctacttcata ataagaacgt gggtcgtctg gtccgaccgc ggtgggagta
      181 atcacaactt tggcaccata tgcacgtaac aataaaatct tttcgttgct catcttatcg
      241 ggcatcacga agatggtttt atagcccttc agtgctgccg caattgccaa ccccacccct
      301 gtattcccgg aggtcgcctc gacgatggtg cccccaggtt tgatctctcc acgtttttct
      361 gcttgttcaa taatattcgc ccccacccta tctttgaccg acccgccggg attcataaat
      421 tccagcttgg catatagtgg aacaggcagg tcttttccaa ttcgttccag tttcacgaga
      481 ggggtatttc ccatcgctcc aagtacattg ttgaatacac gtttctcttt ggattctggc
      541 aatcctgtcg tcattctctc tcctttggtt ttaggggaat tctaatcgac ttcactcacc
      601 ttccactgta ttataaaccg agggtaccgt aaaacaaaaa gcggccgcct tttcaggcag
      661 ccgcttggca tacacaaaaa actttattga accgaatagc tgagggtggt gttcaacgta
      721 ccgttgaaat acacttccac tttataagta ccgactggcc aaccatcggt gggcggttcg
      781 aagtagaaat agaccgagtt gatattgtcc tgatctacat ccacagcagc ttcacccaca
      841 aagaagttcg ggtcaaaacc atctacctga tcgacgaaca aacgagcagt aacattgtta
      901 ccaaccacgc cattggaaag atcacctaca acataaacgg tgtcaaaaga cccgaaggtg
      961 gtggatagtt gggtaccatc ctgatcggtt gccgtgcgga cgttcgagaa cgtaggttcg
     1021 ccagcggcgc aggcgagctg aacagcaact aacataattg cagccagcaa gatcgacagt
     1081 tttttcgctt tcattttggc attctcctca taatataaat gtaggcaggt agccccgcca
     1141 agtgtcgaca ttatacctaa actattacga acttccctcc cctaaaaggg ctattcaatt
     1201 tcaatataaa gccgcttatt gatcgcgcct ttactcttat attcaaccac tttgatggtt
     1261 ccaacttcag aggtgttcgc cacatgcgtt ccgccgtcgg cttgcaaatc taagcctaca
     1321 atctcgaccg tgcgcacttg catgattcct tctgggagga ggttgatctt ggtgcgaatc
     1381 aaatctggga tttgaaacgc ctcttccctc gggaggatct tgactctgat ctcatggttt
     1441 ttcttcacct cggcattaac cgccgcctca atctcgcgga cgagttctgc gcgcatggtc
     1501 tcaaattcaa agtccatacg ccctttgagc ggctccatat caccaccagt aaccttggca
     1561 ccataatcgc ggaagaccgt tccgcataag atgtgcagag cggtatgggt gcgcatcagc
     1621 ttatgtcgcc gcacccagtc taaggtcccg agagaggcgg agccgggcgt tggcaaaggt
     1681 tcggttccac cgagaacgtg aaggacgtca tcgccctgct tcttaacttt ttccactggg
     1741 tatttcacgc cattgacctc cagcgtgcca aaatcgcagg gctgacctcc cccgccaggg
     1801 taaaaagcag aacggtcgag aacgacagca cgagcttcag catcgacaga tgtgatgacg
     1861 gctgaaaact ctttgagata ggcatccgtt tggtagagga gttcggtcat ggctcaatta
     1921 tacaagatgg aatgaattgg tcagacaaaa ttaggattgg attagagggc tatgcacccg
     1981 cttgactttg gtctttcatc tattgtatag tattctgcgg tacgaaaaaa aaggcaccgc
     2041 caaacacggc tgtgtctttt ctttgcgata atcccatttt atatattatt tcaccgtgag
     2101 gagaagcaat gatcaaagtt agtggtctca caaaagacta tgggtcacgt cgcgccatcc
     2161 acaatctgag ttttgatgcc caacagggcg agattgtggg ctttttgggt cccaatggcg
     2221 ccgggaaaac caccaccatg cgcatcctga ctggttacat gccgcctact gatggcgaag
     2281 cgattgtagc tggttatgat gtggtggaag aatcgttgga agtacgcaag cgcgtcggct
     2341 atttgccgga gtctgtgccg ctgtactacg acatggtcgt ttctgaata
//
LOCUS       DAOQFT010000397         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28778_length_2388_cov_8.462923, whole genome shotgun sequence.
ACCESSION   DAOQFT010000397 DAOQFT010000000
VERSION     DAOQFT010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28778_length_2388_cov_8.462923"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..553
                     /locus_tag="PLR93_09915"
     CDS             131..553
                     /locus_tag="PLR93_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27326.1"
                     /translation="MLPETLIEVHEHTKPDYKPLVDYQSWRVALMNYTDDLIPEKINR
                     MQKHTETDEVFVLMAGHCILFLGEGEETVTKVHAVDMELYKLYNVKRGVWHSHTFSKD
                     ARILIVENRDTVVENSPFVDLSPSQCTEVLELTKQLWK"
     gene            complement(588..1436)
                     /locus_tag="PLR93_09920"
     CDS             complement(588..1436)
                     /locus_tag="PLR93_09920"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNH27327.1"
                     /translation="MKAELFFDAKSNLGEGPSWDAKTQTLYWVDIFEKRVYAGNEILA
                     QFDEYVGFVAPTKSGHLIVGKRASFVDLDIATSQQTVLVTLNESATNRMNDGKCDPSG
                     RLIAGTMDLNETDPTGSVYSYDGKSTRVLFRDVTISNGMAWSPDYKTFYYIDTTTCEV
                     RAYDYDIKTGEIANRRRAFEVPKSLGWADGMTSDVEGNLWIAMWGGAQVTRWNPNTGQ
                     LLEQVSVPALHSSCPVFGGKDRNELYITSARKGMSEADLNKYPLSGGLFKVDTKYEGM
                     PTFEFG"
     gene            complement(1436..2362)
                     /locus_tag="PLR93_09925"
     CDS             complement(1436..2362)
                     /locus_tag="PLR93_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNH27328.1"
                     /translation="MLSIYKTTENGLEKLDAIANGTWVNVIDPTPDEMEKLVGWGMEM
                     DYINYSLDQDEMARMERDEDYTFILLRIPIHQPESDIPYNTVPLGIMILGNKIITVCR
                     FDSDIFRALTNGKHRYLKTGKRYRLTLYIFLETAVRYLNLLREINRATEAVEDRLQKS
                     TQNREVLELLKYQKSLTYFATALRSNEVMMERVQKTQLFNYYEEDQDLLEDVLTENQQ
                     AIQMTSIATEILSGMMDAFASIISNNLNVVMKALAALTIILNMPTIVASFYGMNVDLP
                     GEGHPLAFLTVIGLSLALTALATYIFYKRNWF"
BASE COUNT          665 a          562 c          538 g          623 t
ORIGIN      
        1 aggtcaaaaa tcattccccg cgattttgga agttggtaga atcgctctgc cctgacttta
       61 aacatcaccg caagtggtta cgcgaccacg gcgaaacaat gaccttataa cttttctctg
      121 gagaatagcc atgctgcccg aaactctcat cgaagtccac gaacatacca aaccagatta
      181 caaacctctt gtcgattatc agtcttggcg agtggcacta atgaattata cagatgacct
      241 aatccccgag aaaatcaatc gcatgcaaaa gcatacagaa accgatgagg tctttgtgct
      301 tatggctggg cattgtattt tatttctcgg cgaaggcgaa gagacggtaa ccaaagtcca
      361 tgccgtggat atggagttgt acaaacttta taacgttaag cgtggcgtat ggcattcgca
      421 taccttcagc aaagatgccc gcattttgat cgtggagaat cgcgataccg ttgtggaaaa
      481 ttcgcccttt gtggatctat cgccaagtca atgcacagaa gtgctggaat taacaaaaca
      541 attatggaag taaaacgtaa gagcgaggaa accccgccct tacgttttta cccaaactca
      601 aaagtgggca tgccctcata ttttgtgtca actttgaaca acccgccaga gagcgggtat
      661 ttatttaagt ccgcttcgct catgcctttg cgagcagagg tgatatacaa ctcattacgg
      721 tccttgccac caaagaccgg gcaggaagag tgcaaagcag ggacagaaac ctgttccaag
      781 agttgaccgg tattcgggtt ccagcgtgtg acctgtgccc caccccacat ggcgatccat
      841 agattgcctt caacatcaga ggtcatgccg tctgcccagc caagggattt gggaacctca
      901 aaagcacgcc ggcggttcgc gatttcacca gtcttgatat cataatcata ggcgcggact
      961 tcgcaggtag tcgtatcaat gtaatagaag gttttatagt ctgggctcca tgccatgccg
     1021 ttggagatgg tcacatcacg gaacaaaaca cgggtggatt ttccatcata ggaatagaca
     1081 gagcctgtgg gatccgtctc gttcaaatcc atggtgcctg caatcaagcg cccagatggg
     1141 tcacatttgc catcgttcat acggttggtg gcagattcat tcaaagtgac aagcacggtc
     1201 tgctgggagg tggcaatgtc caagtccacg aaagaggcgc gtttgcctac gatgagatgt
     1261 ccgcttttag taggggcaac aaatccaaca tattcatcaa attgagcgag aatttcgtta
     1321 cctgcataga ctcttttttc aaaaatatca acccaataca aggtttgagt ttttgcatcc
     1381 caagatggac cttcgcccaa gtttgatttt gcgtcaaaaa aaagttcagc tttcattaga
     1441 accaattcct tttatagaag atataagtgg ccaatgccgt gagcgccaac gacaagccaa
     1501 taaccgttaa gaatgccagc gggtgtcctt ctcctggcaa atccacattc atgccgtaaa
     1561 acgatgcaac gatggtgggc atgttgagaa taatcgtcag tgccgcaagc gctttcatca
     1621 ccacgtttaa gttattcgag ataatcgaag cgaaggcatc catcatacca gagagaattt
     1681 cagtagcgat gcttgtcatc tgaatcgctt gctgattttc cgtgagcaca tcttcgagca
     1741 agtcttgatc ttcttcataa taattgaaga gttgagtctt ttgtacgcgt tccatcatca
     1801 cttcgttaga gcgcaacgcg gtcgcaaagt aggtaagact cttttgatac ttaagcaatt
     1861 caagcacttc gcgattctga gtagattttt gcaagcggtc ttccacggct tcggtagcgc
     1921 gattgatctc acgcaacaag ttcaaataac gcacagctgt ttcgagaaag atataaagcg
     1981 tgaggcgata acgcttgcca gttttcaaat agcgatgttt gccattggtt aatgcgcgaa
     2041 agatatcact atcgaagcgg caaactgtga tgatcttatt gccaagaatc ataatgccaa
     2101 gcggaacggt gttataagga atatcgctct ctggttggtg aatgggaatg cgaagaagga
     2161 tgaaggtgta atcttcatcg cgttccatac gcgccatttc atcttgatca agtgaataat
     2221 tgatatagtc catctccatc ccccaaccta ctaacttttc catttcatcc ggcgttgggt
     2281 caatcacatt aacccacgtt ccattcgcaa tcgcatcgag cttctccagc ccgttctcgg
     2341 tcgttttgta gatactcagc ataacagcct cctacgtgga atcatcac
//
LOCUS       DAOQFT010000398         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28871_length_2384_cov_7.373122, whole genome shotgun sequence.
ACCESSION   DAOQFT010000398 DAOQFT010000000
VERSION     DAOQFT010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28871_length_2384_cov_7.373122"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLR93_09930"
     CDS             complement(<1..214)
                     /locus_tag="PLR93_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27329.1"
                     /translation="MTNETKQKVREFYDEIGWMQEEDGNYQNARYEDLRPVSREYIHK
                     TRLRVMNGLIPTGRFILDAGSGPVQYE"
     gene            complement(224..1864)
                     /locus_tag="PLR93_09935"
     CDS             complement(224..1864)
                     /locus_tag="PLR93_09935"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27330.1"
                     /translation="MPKPNPLREILGDLPFTAEIDWMLRSKNRPRKDHYNLTRLLTSL
                     PDAVKVVKPYAESAPRGKNVLFFATLHYWIEQSAYLGLVLAGMGHDVTLLTLPYSEWH
                     KEKDKFTQRQRILHTHDALKMLSPLVKHISMLDMQSSSRASHGTLHAHAASELPERIQ
                     TDIKEISLWDVQYTLMREEVDMNDAGDRALYDLRVERNTFAAKAALQYMQANKPDVVL
                     IPNGLILEMGIVFRVARYLGIDAVTYEFNDQREQIWLAQNSSIMQQDTDYLVEARCSQ
                     PMTDAMFERVADLENARRGARVWGKSKRLWQYVSSQGADVTRKQVGLDDRPVVMLAAN
                     VLGDSLTLGRDIFASSMTEWITKTVQYFAKRTDVQLVIRVHPGEKLVPQAKSMATVVK
                     EALPELPSHIHVIGALDKVNTYDLIEIADVGLAYTTTVGLETAMNGRPVISCGQTHYR
                     GRGITIDPNTWDEYYATLEKVLSNIPAHQMNEKQIEFAWNYAYRFFFEYPRPFPWRLM
                     NFWDDLAEHPLESVLSPEGMARFKKTFDFLVCEPFTWD"
     gene            complement(1903..>2384)
                     /locus_tag="PLR93_09940"
     CDS             complement(1903..>2384)
                     /locus_tag="PLR93_09940"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HNH27331.1"
                     /translation="DADEVPAASAPFMPISLKVKERSGKIVDTIRSTEKVTVEFEYKL
                     SSPVTGLRVGLYISTMRGEYVFTSFDTDTPELYEKFDSRNAGHYISRAELPADIFNEG
                     RYMVGVNASSFGVRRYFMDENALAFNVDISGAPGTQWGEPRVGPIRPRLNWKIEKID"
BASE COUNT          623 a          609 c          546 g          606 t
ORIGIN      
        1 cctcatactg gacaggacca gacccggcgt caagaatgaa gcgaccggtg gggatgagtc
       61 cattcatcac gcgcagacgc gtcttgtgga tgtattcgcg tgagacgggg cgcaagtctt
      121 cgtaacgtgc attctgataa ttgccgtctt cttcctgcat ccagccgatc tcatcataaa
      181 attcgcggac tttttgtttg gtttcgttag tcatttattt ttcttaatcc catgtaaaag
      241 gttcgcaaac cagaaaatca aaggtcttct taaatcttgc catgccttca ggacttaaca
      301 cgctttccag agggtgctcg gcaagatcat cccaaaagtt catcaagcgc catgggaagg
      361 ggcgtggata ttcgaagaag aaacgatagg cataattcca cgcaaattca atttgctttt
      421 cattcatttg atgggcagga atattgctta acactttttc aagagtggca tagtattcgt
      481 cccacgtatt tgggtcaatc gtaatgccgc gcccacgata atgggtttgt ccgcaagaaa
      541 tgacggggcg tccgttcatg gcggtttcga gtccgaccgt tgtggtgtag gctaaaccga
      601 catcagcaat ttcgattaaa tcatacgtat tgactttatc taacgcgcca atcacgtgaa
      661 tatggctcgg cagctcgggc aaagcttctt tcactaccgt cgccatagac ttggcttgcg
      721 gcaccaattt ttcacccgga tgaacacgga tcaccaactg aacatccgtc cgcttggcaa
      781 aatattgaac ggtcttggta atccattcgg tcatggaaga ggcgaagatg tcacgcccaa
      841 gcgtgaggct atcacccagc acgttcgcag cgagcatcac tactggacgg tcatccaaac
      901 caacttgttt gcgagtgaca tctgcccctt gcgaagagac atattgccac aagcgtttgg
      961 acttgcccca cacacgcgca ccacgacgag cattttcaag atctgctaca cgctcgaaca
     1021 tagcgtccgt cattggctga gagcagcgtg cctctaccaa ataatcggta tcctgctgca
     1081 tgatggatga attctgcgcc aaccaaatct gttcgcgttg gtcattgaat tcatatgtga
     1141 cggcgtcaat gccaagatag cgcgccacac ggaacacaat tcccatttcc aagatcaaac
     1201 cgttcgggat caaaaccaca tccggtttat tcgcttgcat atattgaagc gcggctttgg
     1261 cagcaaacgt attgcgttcc acacgcaggt catacaaagc gcgatcccct gcgtcgttca
     1321 tatccacttc ttcacgcatc aaagtgtatt gcacatccca aagggaaatc tctttgatat
     1381 cggtttgaat tctttcaggc aattcacttg cagcatgagc atgcaacgtc ccatgagaag
     1441 cacgcgaaga ggattgcata tctaacatag aaatatgctt cacgagcgga gataacattt
     1501 tcaatgcgtc atgcgtatga aggatgcgct gacgttgggt aaatttgtct ttttctttat
     1561 gccattcaga atagggcaaa gtcaacagag taacatcatg ccccatgcct gccaacacca
     1621 aacccaaata tgccgattgt tcgatccagt agtgaagcgt ggcaaagaac aaaacatttt
     1681 ttccacgcgg tgcgctctcg gcataaggct ttaccacctt cacggcatct ggcaaggatg
     1741 tgagcaagcg cgtcaggttg tagtgatcct tgcgcggacg attcttcgag cgaagcatcc
     1801 aatcgatttc ggcagtgaag ggcaggtcgc ccaaaatttc gcggagtggg ttaggttttg
     1861 gcatgaattt tcaaaaggtc atttttgtaa ggtgacctgc gattaatcta tcttctcaat
     1921 cttccaattc aatcgtgggc gtatcggtcc cacacgcggc tcgccccatt gcgtaccagg
     1981 agcgccagaa atatccacat tgaacgccaa cgcattctcg tccatgaaat agcgacgcac
     2041 accaaatgag ctcgcattca cgccaaccat ataacgacct tcgttgaaaa tatcggcggg
     2101 caattccgcg cggctgatgt aatgacccgc attacgcgag tcaaattttt cataaagttc
     2161 aggggtgtcg gtatcgaatg aagtaaacac atactcgccg cgcatggtgc taatatacag
     2221 acccacacgc aaaccagtca caggcgagga taacttgtac tcaaactcaa ccgtgacttt
     2281 ttctgtagag cggatggtat cgacgatctt tccgcttctt tctttaactt tgaggctgat
     2341 cggcataaaa ggcgcactcg ccgctggaac ctcatctgca tccc
//
LOCUS       DAOQFT010000399         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28873_length_2384_cov_6.684414, whole genome shotgun sequence.
ACCESSION   DAOQFT010000399 DAOQFT010000000
VERSION     DAOQFT010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28873_length_2384_cov_6.684414"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLR93_09945"
     CDS             complement(<1..314)
                     /locus_tag="PLR93_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNH27332.1"
                     /translation="MNQSLWYKNAVFYQISVRAFKDSDGDGHGDLQGLTEKLDYLRDL
                     GVDCIWIMPIYPSPLRDDGYDIADYYSVDKTYGSLDQLKALIEAAHQRGIRIITDLVL
                     NHT"
     gene            complement(360..1229)
                     /locus_tag="PLR93_09950"
     CDS             complement(360..1229)
                     /locus_tag="PLR93_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNH27333.1"
                     /translation="MEKISHLIRKIKLGSTLGMLVLVTIWSIPTVGLLITSLRPSSVA
                     EISPWWEALANPLGQNWTIDAYQTSLEKGMLGSMINTIAVTVPSTVLPLMIAANAAFA
                     FTFFKFKGKEIIFAVLVGLMVIPLQSSLIPVLRWMVVFQKAAHIKLTGTFVSAWLVHG
                     AFALPLAIYTLRNYMMTLPHELIESAKVDGASYYQIFWKLVLPLSMPAVASFAIFQFL
                     WVWNDYLIAFIFVGEQKAVMTYRLLRLLGQYGEGWRDVAAGSFVSLIIPLIVFFSLQR
                     FIVRGLLAGSVKG"
     gene            complement(1232..2212)
                     /locus_tag="PLR93_09955"
     CDS             complement(1232..2212)
                     /locus_tag="PLR93_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNH27334.1"
                     /translation="MGDIIFGLYSIVLGIAVSYALYWLLNFLLSLLPQRIFQKVSWLS
                     FLTPAAVLVIVVLIAPLFQTVIWSFKDDKVKEWVGFKNYVDLFTDIRFLGILLNNFLW
                     VLVVPAITVAIGTAAATLSNQVGPTREKIFKSLIFMPMAISFVAASTIWAYFYAYVPP
                     GRPEIGFLNSVVISLGFEAQPWLTMDQGRLNSFLLMIVLIWLQVGYSMVVISAAIKNV
                     PEETLEAAQLDGANAWNIFSKIVLPQVWGTVMSVFVTVLILVMKIFDIVLAMTRGNFT
                     TSVLAFEWYKQFFENSNIGPASAVVTVLVILIGPLMYLQIRTVQQQELLR"
BASE COUNT          750 a          600 c          506 g          528 t
ORIGIN      
        1 gtgtgattca gcaccaggtc cgtgatgatg cggataccgc gctgatgcgc cgcctcgatc
       61 aacgccttga gctggtccag cgacccgtag gttttatcca cgctgtaata gtcggcaatg
      121 tcgtagccat cgtcacgcaa cggcgaaggg tagatcggca tgatccaaat gcagtccaca
      181 ccgaggtctc ttaaatagtc gagcttctcg gtcagacctt gcaagtcacc gtggccatcg
      241 ccatcgctat ccttaaatgc acggacggag atctgataaa aaactgcgtt cttgtaccaa
      301 agagattgat tcatataaaa agtggactgg ttgcccagtc cacttgtcct tttggaaagt
      361 tatcctttga ctgagcccgc cagcaacccg cgtacaataa agcgttgcaa gctgaagaac
      421 acgatcaacg gaatgatgag agagacgaag gaacccgccg ccacatcgcg ccagccttcg
      481 ccgtactgac ccaacaatct caacagacga taggtcatca ctgccttttg ttcacccaca
      541 aaaatgaagg cgattagata atcgttccaa acccacaaga attggaaaat ggcaaaggaa
      601 gccacagcag gcatggacaa cggcaacacc aacttccaaa aaatctggta atagctggca
      661 ccatccacct tagcagattc gattagctca tgaggcaggg tcatcatata gttacggagg
      721 gtatagatcg ctaatggcaa cgcaaatgcc ccgtgaacaa gccaggcgct aacaaatgtg
      781 cccgtaagtt taatatgagc agctttttga aacacaacca tccatctcag cacagggata
      841 agcgaagact gcaatggaat aaccattaaa ccgaccaaaa ccgcaaagat aatttcctta
      901 cctttaaact tgaagaaggt aaatgcaaat gcggcattgg cagcaatcat caacggcaaa
      961 accgtggagg gtacagtcac tgcgatcgtg ttgatcattg accccaacat ccccttttca
     1021 agggaagttt gatacgcatc aattgtccag ttctgcccca acgggtttgc cagagcttcc
     1081 caccaaggag atatttcagc aacagaactc gggcgcagag atgtgatcaa caaacccaca
     1141 gtgggaatgg accagatggt caccagaacc agcatcccca atgtgctacc taattttatt
     1201 tttcgtatca ggtgagaaat tttttccatg ttcatcgcaa caactcctgt tgttgtactg
     1261 tacggatttg caaatacata agcgggccaa ttaatatgac caacactgtc accacagcag
     1321 aggcgggtcc gatgttggaa ttctcaaaga attgcttgta ccattcaaac gccaatacac
     1381 tggtcgtgaa atttccacga gtcatggcaa gtacaatgtc aaatattttc atgacaagaa
     1441 ttaacacggt cacaaagacc gacattacgg tgccccatac ctgaggcaga acaatcttgg
     1501 aaaaaatatt ccaagcattg gcgccatcca attgggcagc ttccagggtc tcttcgggta
     1561 catttttaat cgcggctgaa atcaccacca tggaatagcc cacttgcagc cagattagca
     1621 caatcataag taaaaaggaa tttaaacgac cctgatccat cgtcaaccaa ggttgcgctt
     1681 caaatcccag ggatataaca acagaattta aaaagccgat ttccgggcgt cccggcggaa
     1741 catatgcata aaaataagcc caaatggtag atgcagccac aaagctgatc gccattggca
     1801 taaagatcaa tgatttgaaa attttctcac gggttggtcc cacctgattg gaaagcgtgg
     1861 ctgcggcagt accaattgcc accgtaatgg caggcacaac caacacccat aggaagttat
     1921 tcaacaatat ccccaaaaaa cgtatatcgg taaacaagtc tacataattt ttgaacccca
     1981 cccattcttt tactttgtca tctttgaaag accagatcac cgtctggaat agcggtgcaa
     2041 tcaaaaccac tatcaccaaa accgctgctg gcgtcaaaaa agacaaccaa ctcacttttt
     2101 gaaaaatacg ttggggaaga agggaaagca ggaaattcag aagccagtac agagcatagc
     2161 tgactgcaat accaaggact atggaataaa gcccaaaaat aatgtcgccc atatctcaat
     2221 ctccattcat tcggatttat cgattactac ctagtcacca tggggaaacg agtaaaaaag
     2281 atgtggcacc gaccccatca agaacaagag tcggcgccac tattttacct aacttaccgt
     2341 acgactacgg gtacttgctg tcaatgcgag cggcagcgtc agcc
//
LOCUS       DAOQFT010000400         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28897_length_2383_cov_5.154639, whole genome shotgun sequence.
ACCESSION   DAOQFT010000400 DAOQFT010000000
VERSION     DAOQFT010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28897_length_2383_cov_5.154639"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLR93_09960"
     CDS             <1..237
                     /locus_tag="PLR93_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27335.1"
                     /translation="LPLVTLIALDLPSLFAGALFVETIFSWPGMGRLFWDAAKGRDYP
                     VLLGIVMLTAVLIILCNIIADLAYGWLNPQVKYE"
     gene            230..1132
                     /locus_tag="PLR93_09965"
     CDS             230..1132
                     /locus_tag="PLR93_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27336.1"
                     /translation="MNESSPLIKEQSMAAVITRRFFKHKLAGVAITVLALLVLFSALA
                     SLTPYEPTEQNPTNIFQKASGVHWFGTDELGRDVFTRILYGGRVSLAVGLLSTLLSIS
                     LGVVVGALSGYFGGLLDSTLMRITDAFLTFPTIFVLIILGAFLREQQLYILQNSIFVV
                     IIIIAALSWMWPARLVRGLFLVLREKEFVTASRAMGGSDLRIIVRHILPNCVGPILVS
                     GTLQMASAIITESGLSYLGFGVQPPTPTWGSILSTAQNQVFRAPWLAFYPGLMIFVTV
                     MAINYIGDGLRDAFDPYVIHTKSQ"
     gene            1159..>2383
                     /locus_tag="PLR93_09970"
     CDS             1159..>2383
                     /locus_tag="PLR93_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH27337.1"
                     /translation="MKLKLFTMLVILGLVLSACSSGGNAQATESTSGGGGGGTVTLII
                     PEEPTTLNFYLSDAAIVRQVADSTSMTGLVTIDESGNYVPMLADGLPTISDDHLTVTW
                     KLKDGLKWSDGEALTSDDVRFTVEVLSNPDSGALVGTSGFDMISSVETPDDLTTVLTY
                     SEPYPGYLDQFAYGLFPRHATGAPEEMSSWDWNRAPVTAGPFILSNWESGVSITLTRN
                     PYYFEEGKPYLDSIVFAIVPEPAAQTAMMMNGEAQVHLWPGEFKADYDSMLAGKAQQY
                     LIPGIWNMAIDFNLSAPFDGDPSAAAPHPILGDIRVREAIASAINYQALQQDVLQGSV
                     GDSTNPFAYGWYECKLPRKYGYDVEAANKLLDEAGWVMGDDGIRVAKGAMYAKDGTRL
                     SLELQGYTAFDPLQLT"
BASE COUNT          531 a          594 c          611 g          647 t
ORIGIN      
        1 ctgccactgg ttaccttgat cgcgcttgac ttgcccagtc tttttgcggg tgcgctattt
       61 gtagagacca tcttctcctg gcccggtatg gggcgtctct tttgggacgc ggcaaagggg
      121 cgagattacc ccgtcctcct tgggatcgtg atgctcacag ctgttctaat catcctctgc
      181 aacatcatcg cggacttggc atatggctgg ctcaacccac aggtgaaata tgaatgagtc
      241 gagtccgctt atcaaagaac aaagcatggc agctgtcatc acgcgtcgct tcttcaagca
      301 caaactggcg ggtgttgcca ttacagtctt ggcattattg gtgttgttct cagcgttggc
      361 aagcctaaca ccctatgaac ccacagaaca gaatccgacg aatatttttc aaaaagcatc
      421 cggcgttcat tggtttggca ccgacgaact tggtcgggat gtgttcactc gcatcttata
      481 tggcgggcga gtctcgttgg cagttggctt gctatccacg ttgttatcca tttcgttagg
      541 ggttgtcgtg ggtgcattaa gtggctactt tggcggcttg ctcgactcga ccctgatgcg
      601 catcacagat gcttttctaa ccttccccac catctttgtg ctcatcattc ttggcgcctt
      661 tttgcgtgag cagcaattgt acattttgca aaacagcatc ttcgttgtca tcatcattat
      721 cgccgccctc tcgtggatgt ggcccgcccg cctcgtacgc ggactgttcc tcgtgttgcg
      781 tgaaaaagaa tttgtcaccg cctcgcgtgc catgggtggc agcgatttgc gcattattgt
      841 tcgccatatt ttgccgaatt gtgtcggtcc gatcttggtg agcggaactt tgcaaatggc
      901 ttcggcaatc atcaccgagt ctggcttgag ttatttaggg tttggtgtgc agcccccaac
      961 gcctacatgg ggcagcattt tatccactgc acaaaatcaa gtcttccgtg cgccgtggct
     1021 ggcgttttac ccaggcttga tgatcttcgt caccgtgatg gcgatcaact atattggcga
     1081 cgggttaaga gatgcgttcg acccgtatgt gatccatacc aaatcgcaat agagtttata
     1141 aatcaaaaag gagaagagat gaaactcaaa ctgtttacaa tgcttgttat tttgggtctg
     1201 gttctgagtg cttgttccag cggcggaaat gcccaagcaa cagaatccac tagcggcgga
     1261 ggtggaggcg gcaccgtcac cctcatcatt ccagaagagc ccaccacgct gaacttctat
     1321 ctatcagatg ctgcgattgt ccgacaggtt gcagattcaa cttcgatgac cggtctagta
     1381 accatcgacg aatctgggaa ttatgtgccc atgttagcag atgggctacc tacgatttca
     1441 gatgaccacc tcactgttac ttggaagttg aaagatggtt tgaaatggtc tgatggtgaa
     1501 gcactcacct cagatgatgt ccgctttacc gttgaagtgt tatccaatcc cgattctggg
     1561 gcgttggttg gcaccagcgg gtttgacatg atctcaagtg tcgaaacgcc agatgacttg
     1621 accacggttt taacctattc agaaccttac ccgggttatc tcgatcaatt cgcttacgga
     1681 ttgttccccc gccacgccac gggtgcacct gaagaaatgt cgagctggga ttggaatcgc
     1741 gcgccagtta cagcaggtcc tttcattttg agcaattggg aatcaggcgt gagcattacc
     1801 ctcacgcgca acccttatta ttttgaagaa ggaaaaccct atctcgatag catcgtgttt
     1861 gcaattgtgc ctgaacctgc tgcgcaaacc gctatgatga tgaacggtga agcccaagtt
     1921 catttgtggc ccggtgaatt caaagcagac tatgacagca tgctggctgg caaagctcag
     1981 caatatctca ttcctggcat ttggaatatg gcgatcgatt tcaacttgag cgcaccgttc
     2041 gatggcgatc cgagtgcagc ggctccacac cccatcttgg gtgatattcg cgtccgcgaa
     2101 gcgattgcaa gcgccatcaa ttaccaagcc ttgcagcagg atgtattgca gggcagtgtc
     2161 ggtgattcga ccaatccatt cgcctatggt tggtatgaat gtaaacttcc gcgcaagtat
     2221 gggtatgatg tggaagcagc caataaatta ttggatgaag cgggttgggt gatgggcgat
     2281 gatggcatcc gcgtggcaaa aggtgccatg tatgccaaag atggaacccg cctctctctg
     2341 gaacttcagg ggtataccgc tttcgatcct ttgcagctca ctg
//
LOCUS       DAOQFT010000401         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28911_length_2382_cov_6.904168, whole genome shotgun sequence.
ACCESSION   DAOQFT010000401 DAOQFT010000000
VERSION     DAOQFT010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28911_length_2382_cov_6.904168"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="PLR93_09975"
     CDS             complement(<1..1797)
                     /locus_tag="PLR93_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27338.1"
                     /translation="MMKLMKFVKPYRWMLLFAIALIFAQANLDLALPDYLSRIVNTGI
                     QQGGVEDSMPEAIRASEMDKVAIFLNAQDKEDILASYTLVTDSSPDYETYLEKYPTLA
                     DEPIYVLNDISQSEIDRLDPAMAKALLTVSGIEKALKDPEAAAQMGGAFGGFDPSKLP
                     PGMDLFTMLGKLPAAQLEQITSSVDEKFSALGDTMIVQAGVNVVRDEYKALGMDTEQI
                     QNNYILTAGAWMIGLTLLSGVATIIVGYLSAKIAAGMARDIRRDVFQHVESFSSTEFD
                     KFSTASLITRTTNDVTQIQMVSMLMIRMMFYAPLMGVGGIIKVISNESPLAWIIGVAV
                     LMLISLIAVVFSLSLPKFKIIQKLTDRINLVARENLTGMMVVRAFTMENFEEKRFDVA
                     NKDLTSVSLFINRVMVVMMPLMMLIMNVMLLGVIWFGAHEVAASNMQVGDMMAFMQYS
                     MQIVMSFLMLSMMFIMIPRASVSADRIHEVLSTVPYIHDSTAAKKFPEPFKGEVEFRN
                     VSFRYPGGDIDAIQNVSFVAKPGQTTAFIGSTGAGKSTIVNLIPRFYEVSDGSIFIDG
                     VDIRDVTQHDLREKIGYVPQKSALFSGTIESNM"
     gene            complement(1882..2331)
                     /locus_tag="PLR93_09980"
     CDS             complement(1882..2331)
                     /locus_tag="PLR93_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH27339.1"
                     /translation="MSDSVQFSQSLRQWVDIFMHHSMRGWHHFVKSTGLTMPQFSILM
                     QLHYKGRIGMSEVSEQFGVSAAAASQLVDKLVQAGFMERAEDPSDRRAKLIQLSPNGK
                     KLIETGISERYRWMDGLTSKLSADDKAKVVDALQVLTEAARQLDETA"
BASE COUNT          598 a          563 c          632 g          589 t
ORIGIN      
        1 catattgctt tcgatggtgc ccgagaacag cgcagacttt tgcggcacgt agccgatttt
       61 ttcgcgcagg tcgtgctgag tcacatcgcg gatgtcaacg ccgtcaatga agatggagcc
      121 atccgaaact tcgtagaagc gcggaatgag gttgacgatg gtggactttc cggctccggt
      181 tgacccgatg aatgcagtgg tctgcccggg ctttgctaca aagctgacgt tttgtatcgc
      241 atcaatgtca ccacccggat agcggaatga gacgttgcgg aattcaactt cgcccttgaa
      301 aggttcagga aatttcttgg ctgccgttga atcgtggatg tacggcacgg tgctcagcac
      361 ttcatgaatg cggtctgcag aaacagaagc acgcgggatc ataatgaaca tcatcgagag
      421 catcaggaag gacattacga tctgcattga atactgcatg aatgccatca tgtcaccgac
      481 ttgcatgttc gaagcggcaa cttcatgcgc accgaaccag atcacgccca aaagcatcac
      541 gttcatgatg agcatcatca acggcatcat taccaccatc acgcggttga tgaacaagct
      601 gacggaggtc aggtctttgt tggcaacgtc gaagcgtttt tcttcgaagt tttccatggt
      661 gaaagcgcgg acgaccatca taccggtcag gttttcgcgt gccacaaggt tgatgcggtc
      721 ggtcagtttt tggatgattt tgaacttagg caacgagagc gagaagacga cagcgatgag
      781 gctgattagc atcaacactg ccacgccgat aatccacgcc agcggtgatt cgttgctgat
      841 gactttgatg atgccgccca cacccattag cggagcgtag aacatcatgc ggatcattaa
      901 catgctgacc atttgaatct gggtcacatc gttggtagta cgggtgataa gcgaagctgt
      961 tgagaactta tcaaactcgg tgctggagaa actttcgaca tgctggaaca catcgcggcg
     1021 gatgtcgcgt gccatgcccg cggcgatctt tgcagaaaga tacccaacaa tgatggttgc
     1081 cacaccagag agcagagtaa ggccaatcat ccatgcaccc gcggtgagga tgtagttgtt
     1141 ctggatctgt tccgtatcca tgccgagcgc tttgtattcg tcacggacca cgtttacgcc
     1201 cgcctgcacg atcatcgtat cgccaagcgc agagaatttt tcatccactg agctggtgat
     1261 ttgctctaac tgtgcagcag gaagttttcc gagcatggtg aacaaatcca tgccaggggg
     1321 gagtttgcta gggtcgaaac cgccgaaagc tccacccatt tgggcggcgg cttctgggtc
     1381 tttcaacgcc ttttcaatgc cagaaacggt cagcaaggct tttgccattg ccgggtcgag
     1441 gcgatcgatc tcactctgcg aaatatcatt taacacataa atgggttcat cagcgagagt
     1501 aggatacttt tcgaggtagg tctcgtaatc gggagaagaa tcagttacga gtgtgtacga
     1561 ggcgaggatg tcttccttat cctgtgcgtt caagaagata gcgaccttgt ccatttcact
     1621 ggcgcgaatg gcttcaggca tggaatcttc cacaccgcct tgttgaatac cggtgttgac
     1681 gatgcgcgaa agataatcgg gcagggcaag gtcgaggttt gcttgcgcaa agatcaaagc
     1741 gatcgcaaac aataacatcc accgataagg ttttacgaat ttcattaact tcatcatacg
     1801 gaataaaact ccttaatatc gaaatatcac gatatgttta acaaaagaat gtgtagaaaa
     1861 tttgtagaag gaagagagac tttatgcagt ttcgtcgagt tgacgtgccg cctcagtcag
     1921 gacttggagc gcatccacca ctttggcttt gtcatccgct gaaagtttag atgtgagacc
     1981 atccatccaa cgatagcgtt ctgagatgcc ggtttcgatg agcttcttgc cgttggggga
     2041 gagctgaatc aacttggcgc ggcggtcact ggggtcttcg gcgcgttcca taaagcctgc
     2101 ctgaacgagc ttatccacca gttggctggc agccgctgca gaaacaccga attgctcgct
     2161 cacctcagac atgccaatgc gtcctttgta gtgaagctgc atcagaatac tgaattgagg
     2221 catggtcaac ccggtggatt tgacgaagtg gtgccatccg cgcatggagt ggtgcatgaa
     2281 aatatccacc cattggcgca gagattgtga gaattggact gagtcactca tagttaagcc
     2341 ttctttttag ttaagcgggc ttaactttat gcattagtta ag
//
LOCUS       DAOQFT010000402         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_28977_length_2379_cov_6.456110, whole genome shotgun sequence.
ACCESSION   DAOQFT010000402 DAOQFT010000000
VERSION     DAOQFT010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28977_length_2379_cov_6.456110"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLR93_09985"
     CDS             <1..274
                     /locus_tag="PLR93_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH27340.1"
                     /translation="LIPGYRVAGKTGTAQIPVDGFYDSTQTNTSFIGWGPVDDPKFMI
                     YVWLERPTASPWGSETAAPVFAEMAQRTVVLLDIPPDSIRLQVAQK"
     gene            276..1694
                     /gene="murF"
                     /locus_tag="PLR93_09990"
     CDS             276..1694
                     /gene="murF"
                     /locus_tag="PLR93_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNH27341.1"
                     /translation="MLTLADVLEALTTIRPANASTIITEGAIDSRQVIPGSLFVAIPG
                     EKTDGHDYLTDAFKRGASFALIQRDVDASFRTLDLRAGLSTDATLNFESPLCLRVDNT
                     ISALQQIARFWRRKLPNLRVVGITGSVGKSTTKETVAEVLSLRYRTLKSPGNLNNEIG
                     LPLTILRLGTGYDRAVLEMGFYVPGEIQFLCDIAQPQMGVVSNVGTVHAERAGSQEAI
                     FRGKSELPASLPADGVAILNFDDQWVRKMEEKTKARVFFYGLSPEAHLWADQIEGLGL
                     DGIRFRMHYGKEIIHMRVPMIGRHSIHTALRAAAVGLNDGISWQEILEGLSHGHTQLR
                     LVAARNETGALILDDTYNAAPESMLAALNLLDELDGRKIAVLGDMLELGPYERQGHEM
                     VGMRAAQVAKVLITLGTRGHMIAEAARRAGMKASAIMEYEDIAPIIDWLTKNLNSNDA
                     VLIKGSHGLRMDRIAAALEVRS"
     gene            1691..>2379
                     /locus_tag="PLR93_09995"
     CDS             1691..>2379
                     /locus_tag="PLR93_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNH27342.1"
                     /translation="MRDIALSLSLAGLAFIMTAIWGNPLLRILRHFKLGKIIRIEQPE
                     GHRVKMGTPTMGGVMFIVPVLLLSGLLNAVALIGVTASGVGRSVLLPMVVMVGFAILG
                     AVDDWEGIHGKRKGDGMRARTKFAFQVILALATAYVLKYFIKAPDLYLPGYFFEIQIG
                     WIYVPIAAFIIVAESNAVNFTDGLDGLAGLIAATAIAAFGGIALMQEQIYLARFCFIL
                     VGALFGFLWFNV"
BASE COUNT          536 a          540 c          622 g          681 t
ORIGIN      
        1 attgatccct gggtatcgcg tggctggtaa gactggcaca gcacagattc cagtcgatgg
       61 tttttatgac agtacgcaaa caaacacctc tttcattggt tggggtccag tagatgaccc
      121 gaaatttatg atctatgttt ggctcgaacg ccctactgct tcaccttggg gttctgaaac
      181 tgctgcccct gtttttgctg aaatggcgca gcgaaccgtg gtcttgttag atatccctcc
      241 tgattcaatt cgcctacaag ttgctcaaaa ataatatgct tactctagca gacgttcttg
      301 aagctcttac caccattcgc cctgcaaatg cctctacaat cattacagaa ggtgccatcg
      361 attcgcgcca agtgattccg ggttctcttt ttgttgccat tcccggcgaa aaaacagatg
      421 ggcatgacta tctgacagat gccttcaaga ggggggcttc ttttgcttta atccaacgag
      481 acgtagacgc ttctttccgg accctcgacc tgcgcgctgg tctatcaact gatgccacac
      541 tgaactttga gtctcctctc tgcctgcgcg tggacaatac aatttcagcc ttacaacaaa
      601 tcgcgcgttt ctggcgccgt aaattgccga acctgcgcgt ggtaggaatc acaggaagtg
      661 tgggtaaatc tacaaccaaa gaaactgtag cagaggtgtt aagccttcgt taccgcacac
      721 tcaaaagccc tggcaattta aataacgaga ttggacttcc gctcaccata ttaagactcg
      781 gtacaggtta tgaccgtgct gtgcttgaaa tgggatttta cgtacctggc gagattcaat
      841 ttctttgtga tattgctcag ccgcaaatgg gtgttgtttc aaatgtgggc acggtccatg
      901 cggaacgcgc cgggtcacag gaagctattt tccgcggcaa gagcgaatta ccagcctctc
      961 ttccagctga tggggttgcg attctaaatt ttgatgacca gtgggtccgc aaaatggaag
     1021 agaagaccaa ggctcgcgtt ttcttctatg ggctttctcc agaagcacat ttgtgggcag
     1081 accagatcga agggctgggc ttagatggca tccgcttccg tatgcattac ggtaaagaga
     1141 ttatccacat gcgcgtgccg atgatcggac gtcattccat tcatacggca ttacgcgctg
     1201 ctgctgttgg gctgaatgat ggcatttctt ggcaagaaat tttagaaggt ctctcccatg
     1261 ggcacactca actgcgcttg gtagctgctc gtaatgagac cggcgctttg attttggatg
     1321 atacatacaa tgcagcacct gagtctatgt tggcagcgtt aaatctgttg gacgaattag
     1381 acggtcgcaa gattgccgtg ttaggtgata tgcttgaact tggtccctac gaacgccagg
     1441 gacatgaaat ggtcggaatg cgtgcggctc aagtggcgaa agtcctcatt accttgggca
     1501 cccgtggaca catgattgcg gaagcggctc gtcgagccgg tatgaaggct tctgctatta
     1561 tggaatacga agacattgcc ccgatcatag attggttgac aaagaatttg aattcaaatg
     1621 acgcagtgtt aattaaaggg tctcatggct tacgcatgga tcgcatcgct gctgcattgg
     1681 aggtacgctc gtgagagaca ttgctctttc cctaagcctg gcggggttgg cttttatcat
     1741 gactgccatt tggggaaatc cgttgctgcg tattttgcgg cactttaagt tggggaagat
     1801 catccgtatt gaacagccgg aaggtcatcg ggtgaagatg ggcaccccaa ccatgggtgg
     1861 ggtgatgttt attgtgccgg tcttgcttct tagcggtttg ctgaatgccg ttgccttgat
     1921 tggtgtaacc gccagtgggg tgggtcgctc tgtgttgttg cccatggttg tgatggttgg
     1981 ttttgccatc ctaggtgcag tggacgattg ggaaggtatt cacggcaaac gcaaaggcga
     2041 tgggatgcgc gcccgcacga aatttgcttt tcaagtgatt cttgccttag caacagccta
     2101 cgtgttgaaa tattttatta aagcacccga cctgtattta ccgggttatt tcttcgaaat
     2161 tcaaatcggt tggatctatg tgcctatcgc tgcattcatt attgttgctg agtcaaatgc
     2221 agtcaatttt acagacgggc ttgatggttt agctgggttg attgcggcga ctgccattgc
     2281 cgctttcggt ggaatcgctc ttatgcaaga gcagatctat ctggcacgtt tttgttttat
     2341 cctcgttgga gccttgttcg gctttttatg gtttaacgt
//
LOCUS       DAOQFT010000403         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29146_length_2370_cov_7.025054, whole genome shotgun sequence.
ACCESSION   DAOQFT010000403 DAOQFT010000000
VERSION     DAOQFT010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29146_length_2370_cov_7.025054"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="PLR93_10000"
     CDS             <1..1685
                     /locus_tag="PLR93_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNH27343.1"
                     /translation="YAPWLDMEKLMREHEIPLYALESKRPLACFDLIGFSLPYETLYT
                     NALNILDLAGIPVRNVERDDSHPIIIAGGHSATNPEPMHAFIDAFVIGEGEEAIHDII
                     NSLQKTKGLPREERLRALLSISGIYVPRFYETTYMEDGTVAATTPTVAEAPKLVNKRI
                     VAKLPPPPTKFIVPNIDVVHNRVSVEIMRGCTRGCRFCQAGMITRPVRERTVQEVVDA
                     ADAAVRSTGFEELALLSLSSSDYTYVNDLVDAISKRFEGRKLTVSLPSLRIESVSVDL
                     MEKLKQHRSGGFTLAPEAASERMRRIINKYIPDEDIINTTRDIYARGWTTIKLYFMIG
                     HPSETIEDVQAIADLCKRVLAEGRKVAGWKVKLHAGVSTFVPKPQTPFQWVACDTRDN
                     ILEKQALLKREFFKDKSIKLSWTKPDDTLVEAWLSRGDRRMSEVIYTAWKNGAKFDAW
                     EEGRKFETWMAAFEENGIDHAFYTHRQRRTDEIFPWEHITAAVRKNFLFQDFRMSLEG
                     QIRVDCRLNCFACGILPTFANLRRENPGEGWKCPEVKTPARKVELELPIAGD"
     gene            1694..2221
                     /locus_tag="PLR93_10005"
     CDS             1694..2221
                     /locus_tag="PLR93_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27344.1"
                     /translation="MNKAILLERLQNERDQFELLLNRLGFARQLTMRGVVAGLTVKDL
                     VAEVFSHEQFIADRLSEILHGDTYAPSASFTALENFQREYGYPDYESSLIEKDKHDLS
                     VVDAYKHIEFDEIISQELAVYGNILESIEKLTHHEFLDHDLYNRIAEHTYRPYRRTSS
                     IINRWLKRIASETKK"
BASE COUNT          637 a          632 c          525 g          576 t
ORIGIN      
        1 cctacgcccc ctggctagac atggaaaagc tcatgcgcga acacgagatt ccgctgtacg
       61 cgctcgaatc aaaacgacct ttagcctgtt tcgacctcat cggcttttct ctcccctacg
      121 agacactcta cactaatgcg ctcaacattc tagaccttgc tggcatccca gtccgcaacg
      181 tagagcgtga cgactcgcat cccatcatca tcgcgggtgg acattccgcc accaaccctg
      241 agccaatgca tgccttcatt gatgcgtttg tgatcggcga aggtgaagaa gccatccacg
      301 atatcatcaa ttcgcttcaa aagacaaaag gcttaccacg tgaagaacgt ttgcgtgcgt
      361 tgctaagtat ttctggcata tatgtaccgc gtttctatga aacaacatac atggaggacg
      421 gcactgttgc agccacaacc cccactgttg cagaagctcc aaaactggtt aacaaacgca
      481 ttgtggcaaa actgccgcca ccacctacaa aattcatcgt cccaaacatt gatgtagtcc
      541 ataaccgcgt ttcggtcgaa attatgcgtg ggtgcacccg cggctgtcgc ttctgccagg
      601 cagggatgat cactcgcccg gtccgcgaac gaaccgtaca agaagtggta gatgccgctg
      661 atgctgcagt ccgctctacc gggtttgaag agctagcgct gctgtcgcta tcctcatcag
      721 actacactta tgtcaacgac cttgtggatg ccatcagcaa acggtttgaa ggtcgaaagt
      781 tgactgtctc gctgccttct ctgcgcatcg agtctgtttc ggtagattta atggaaaagc
      841 tcaaacagca tcgctcaggc gggtttactc ttgctcctga agctgccagt gaacgcatgc
      901 gccgtatcat caataagtac attcctgacg aagatatcat caataccact cgcgatattt
      961 acgcccgcgg ttggacgact atcaaactct acttcatgat cggtcacccc agtgaaacga
     1021 ttgaagatgt acaagccatt gcagatctgt gcaaacgcgt ccttgctgaa ggacgtaaag
     1081 tggcgggttg gaaagtgaag ttacatgctg gtgtcagcac ctttgtgcct aaaccacaaa
     1141 caccttttca atgggtggct tgtgacacac gcgacaatat tcttgaaaag caagccttgc
     1201 tcaaacgtga attcttcaag gataaaagca tcaaactaag ctggacaaag cctgacgaca
     1261 ctcttgttga agcctggctt tctcgtggcg accgccgcat gtctgaggtg atctacacag
     1321 catggaaaaa cggtgccaaa ttcgatgcct gggaagaagg gcgcaaattc gaaacgtgga
     1381 tggctgcctt cgaagaaaat ggcattgacc atgccttcta tacccatcgt caacgccgta
     1441 cggatgaaat cttcccatgg gaacatatca cagccgctgt tcgtaagaac ttcctcttcc
     1501 aagattttcg tatgtcgctc gaaggtcaga tccgcgtgga ctgtcgcttg aattgtttcg
     1561 cttgcggaat tctgcccacc tttgcgaacc tgcgccgcga aaaccccggt gaaggttgga
     1621 aatgtcccga agtgaaaacc cctgcccgta aagtagaatt ggaacttccc atcgctggag
     1681 attagaaaca agcatgaaca aagccatcct cctcgaacgc ttgcaaaatg aacgcgacca
     1741 gtttgaactt ttactgaatc gccttggttt tgcgcgtcaa ctaaccatgc gcggagtggt
     1801 agctgggctt actgtcaaag atcttgtagc agaagttttt tcacatgagc aattcattgc
     1861 agaccgttta agcgaaattc tgcatggtga tacctatgct ccatcagctt ctttcacagc
     1921 cctcgaaaat tttcagcgag aatacggtta tcctgattac gagtcttcgt tgatcgaaaa
     1981 agataagcat gacctatctg tggtagatgc ttataaacat atcgaattcg acgaaatcat
     2041 ttctcaagaa ctggctgtat atggcaatat tctagagtca attgaaaaac tgacacatca
     2101 tgaatttctc gaccacgacc tgtacaatcg catcgcggaa cacacttatc gcccctaccg
     2161 ccgtaccagt tctatcatca accgctggct aaaacgcatc gcctcagaga caaaaaaata
     2221 aatatgcgag ttcgaattac attcaccaaa caaggcgcat tgcgctatat tggtcatctt
     2281 gacctgcacc gagtatggga acgggcatta cgccgtgcca gtctgcccat ttcatactcc
     2341 caaggctttc acccccaacc caagatcagc
//
LOCUS       DAOQFT010000404         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29153_length_2370_cov_4.309719, whole genome shotgun sequence.
ACCESSION   DAOQFT010000404 DAOQFT010000000
VERSION     DAOQFT010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29153_length_2370_cov_4.309719"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLR93_10010"
     CDS             complement(<1..285)
                     /locus_tag="PLR93_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27345.1"
                     /translation="MTSNLEALTEINLDDLVSSFGWQHSPILSRLLRIFFRKPAQTFA
                     QHVVEYDDTVGAHGILEGGWNLARRYVQDIRIIGAERIPESAFLALSNHPG"
     gene            complement(282..587)
                     /locus_tag="PLR93_10015"
     CDS             complement(282..587)
                     /locus_tag="PLR93_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNH27346.1"
                     /translation="MNSHALPDIQIIEETDTELEPMYSVIIHNDDVTPMDFVVEVLKQ
                     IFFLGNDRAAEIMLAAHIKGTAYVQSLPRMEAEKRVQHAHQAAGMQGYPLTFTVERE"
     gene            complement(584..1462)
                     /locus_tag="PLR93_10020"
     CDS             complement(584..1462)
                     /locus_tag="PLR93_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH27347.1"
                     /translation="MCRKYADGMDDYGVFLCTIVTMKYETLPHITLPKIGFGTWKIGG
                     GSTPNHAVDEKSLEALRYALEIGYTHIDTAEMYANGHSEDLVGQAVRESGKRREDVFI
                     TSKVVPSHLRYDDVLNACENSLRHLKMDYIDLYLIHWPQAGAKYEDTFKALNKLVRDG
                     KVKHLGVSNFNLKLLKQAQSLSETPIITNQVPFSLGDRSYIKNGVLEYCQQNDILLTA
                     YSPIEEGNLRSHKTLDVIAKAHNATIFQIALAWVVSHPRVITIPMSFNPVHIRENFEA
                     ADIELTQDEIQQLNNL"
     gene            1503..>2370
                     /locus_tag="PLR93_10025"
     CDS             1503..>2370
                     /locus_tag="PLR93_10025"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH27348.1"
                     /translation="MSNWIKNKKFWWIAAGALVVIALLIGTLTGNANASDSAAAETAQ
                     VVSLTLTETVDSTGSLEAEPFANLDWKTSGTVATVNVKPGDFVKAGDVLLTLDPTSTS
                     SSIVSAQADLIAAQEDLDTLLNSGTDLAQAAIDLKDAKEDFEEAEDYLLYLQTNQRVP
                     QTKYLAKLVKRGNGWAYDYDTETFRGPAPEDWIIEAENDLALKKAKYEDAQREYDRLL
                     AGDKSQDVIAAQAKVDAAQATVNTLSIIAPFDGQVLSVEQIAGEVVSTGDLSVNIANL
                     ENLYVDAQVDESD"
BASE COUNT          603 a          559 c          611 g          597 t
ORIGIN      
        1 gccgggatga ttggataagg ctagaaacgc tgactcaggg atccgctccg cgccgatgat
       61 gcggatatct tgaacgtatc ggcgcgcgag attccatcca ccttccaaaa tgccatgtgc
      121 gccgacggtg tcatcatatt ccacaacgtg ttgagcaaat gtctgcgcag gcttgcggaa
      181 gaatatccgc aagaggcgcg agagaatcgg cgaatgttgc cagccaaacg agctgacaag
      241 atcgtcgagg ttgatttcgg tgagagcttc gaggttggat gtcattcgcg ttccactgta
      301 aatgtgagcg gatagccctg catgcccgcg gcttgatgtg cgtgctgtac gcgcttctca
      361 gcttccatgc gcggaaggct ttgaacatat gctgtgcctt tgatatgtgc ggcaagcata
      421 atctccgcag cgcggtcgtt gccgaggaag aaaatttgtt tgagcacttc caccacaaag
      481 tccattggtg tcacatcatc attgtggatg atgacggagt acatcggctc aagttcggta
      541 tcagtctctt caatgatttg gatatctggt aaagcgtgag aattcataga ttgtttagtt
      601 gttgaatttc atcttgagtg agctcaatat ctgccgcttc aaaattctcg cggatgtgaa
      661 ccgggttgaa cgacatggga atggtaatca ctcgcggatg cgacacgacc catgccaatg
      721 cgatctggaa gatcgtggca ttgtgcgctt tggcgatgac atccaacgtt ttatgcgagc
      781 ggaggtttcc ttcttcaatg ggtgaatacg cagttaatag aatatcgttc tgctgacaat
      841 attccaagac tccatttttg atgtaggaac ggtcacccaa actgaaagga acttgattgg
      901 tgatgatggg tgtttcagaa agcgactgcg cctgtttgag taatttcaaa ttgaagttac
      961 tcacgccgag gtgctttacc ttgccatcgc gcaccagttt gtttaatgcc ttgaacgtat
     1021 cttcatactt tgcgccagcc tggggccagt ggatgagata caaatcaata tagtccatct
     1081 tgaggtgacg cagagaattt tcgcaagcgt tcaaaacatc gtcgtagcgc aaatggcttg
     1141 gcacaacttt ggatgtaatg aacacatcct cgcgcctctt gccagattcg cgcacagcct
     1201 gcccaaccag atcttcgctg tgaccatttg catacatttc ggcagtgtca atgtgcgtat
     1261 acccgatctc cagggcatag cgtaacgcct ccagagattt ttcgtccacg gcgtggttgg
     1321 gcgtggagcc gccgccgatc ttccaggtgc caaagccgat cttggggagt gtgatatgag
     1381 ggagggtttc gtatttcatg gtcacaattg tacacaagaa cactccataa tcgtccattc
     1441 cgtctgcata ttttctgcac atttgaaaag tatcctgtag acaatcaaaa aggagaaatc
     1501 aaatgtcaaa ctggatcaaa aacaaaaaat tctggtggat cgccgctggc gcgctcgtgg
     1561 tgatcgcttt gcttatcggt accctgactg gaaatgcgaa cgcctcagac tcagccgcag
     1621 cggaaactgc ccaggtagtt tctcttacgc tgacagagac cgtcgactcg accggcagcc
     1681 ttgaagccga gccgttcgcc aaccttgatt ggaaaacgag tggtaccgta gctactgtca
     1741 acgtgaagcc aggagacttt gtgaaggctg gcgatgtctt attaacgcta gacccaacca
     1801 gcacttcatc tagcattgtc tcagcacaag cagatttgat tgctgcacaa gaagatttag
     1861 atactttact taattctggt accgatctag ctcaagccgc cattgatctg aaggatgcaa
     1921 aagaagactt tgaagaagct gaagattatc ttctatacct tcagaccaat caacgtgttc
     1981 cgcaaaccaa atatcttgcc aagctggtaa aacgtgggaa tggctgggca tacgattacg
     2041 ataccgaaac cttccgcgga cccgcccccg aagattggat catcgaagcg gagaacgatc
     2101 tggcgttgaa gaaagccaag tatgaagatg ctcagcgcga atatgaccgt ttgttggctg
     2161 gcgacaagtc acaagatgtg atcgccgctc aagctaaagt ggatgccgct caagcgaccg
     2221 tcaatactct gagcatcatc gctccgtttg atggacaggt gctttccgtg gagcagatcg
     2281 cgggcgaagt tgtcagcacg ggtgaccttt cggtgaatat tgccaatttg gaaaatctct
     2341 acgtcgatgc gcaggtggat gaatcagaca
//
LOCUS       DAOQFT010000405         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29291_length_2363_cov_5.173310, whole genome shotgun sequence.
ACCESSION   DAOQFT010000405 DAOQFT010000000
VERSION     DAOQFT010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29291_length_2363_cov_5.173310"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLR93_10030"
     CDS             complement(<1..339)
                     /locus_tag="PLR93_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27349.1"
                     /translation="MKPSEALNIAWDAILKNKIRSFLTMLGIIIGVSAVIIMVAISAG
                     TEASIQEQITSLGSNLVFVQSSMMRGGPGQAPEGGLVYDDAAAIETGVDGVTAVVVEQ
                     NSTETVKAGDI"
     gene            complement(326..1063)
                     /locus_tag="PLR93_10035"
     CDS             complement(326..1063)
                     /locus_tag="PLR93_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27350.1"
                     /translation="MSTEHSIIETVDITKVYGMGDISVSALRGVSIQIQKGEFVAIMG
                     PSGSGKSTLMHILGCLSKPSSGQYFLDGEDVSQLDKVQLASVRNQKLGFIFQAYNLLA
                     RTTALQNVMLPLLYNHSQNFTKEEGAQKAKDLLELVGLGGRTNHQPHELSGGQQQRVA
                     IARSLINDPVMVIADEPTGNLDTRSGKEIMNLLHTLHEQGTTIVMVTHDPKTAIHTQR
                     VITLVDGLVDNITHNKKYVEEAAHEAV"
     gene            complement(1060..>2363)
                     /locus_tag="PLR93_10040"
     CDS             complement(1060..>2363)
                     /locus_tag="PLR93_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH27351.1"
                     /translation="MTRSPLVAIATRGDLVIYASGTGTLVSANETELSFTSTGKVTEV
                     NVTVGEKVEAGTQLAKLDDTDAQAAYAEANRAYLELTSPSAIATAQQSIADATSDVSD
                     AKNTLAYIISPEALQAEEKVEEAQTAIAEAEAKLKESPTDTDAKKALEDAQSDLEASQ
                     ELLAQAQGRYTSYYLPKYFTDGKELNAPSEAEILAARADYELAKATLQEAKWLYAALT
                     GEKIPENATGSGLTELEQARLNLESAKATLDGTQIIAPYAGTVMSIDISVGDSVSSGS
                     SAITLADLTQPYLEVYLDESDWSSVVVGYETDVIFDILPDKTFSGVVTQVDPGLYSSN
                     NTSVVRAFVKLTQIDEESFTLPLGTTASVDVIGGRAENAVLVPIEALHKAGDQYAVFI
                     MENDKPILHVVEVGIQDLLYAEIISGVKEGDLVTTGIVETQ"
BASE COUNT          568 a          578 c          548 g          669 t
ORIGIN      
        1 gatatcgcca gcttttacag tttcggtgga gttttgttcc actacgacag cggtcacacc
       61 gtctacaccg gtttcgatgg cagccgcatc gtcatagacg agaccacctt cgggtgcctg
      121 accgggtccg cctcgcatca tggagctttg tacgaatact agatttgacc caaggctggt
      181 gatctgttct tgaatactgg cttctgtccc tgcgctgatg gcaaccatga tgatgaccgc
      241 cgagacacca atgatgattc ctagcatggt caagaaggag cggatcttat tcttgagaat
      301 ggcatcccat gcaatgttca acgcttcaga cggcttcatg ggcagcttcc tcaacatatt
      361 ttttgttgtg ggtaatgtta tctaccaaac catccactaa ggtgatcacg cgctgggtat
      421 ggatggcagt tttagggtca tgcgtaacca tgacaatagt tgtgccttgc tcatgcaaag
      481 tatgcaggag attcatgatc tctttgcctg aacgtgtgtc gagatttcct gtcggttcat
      541 cggcaatgac catgaccggg tcattgatca gtgaacgcgc aattgccaca cgttgttgct
      601 gaccgccaga aagctcatgc ggttggtggt ttgtccgccc gcccaagccg accaattcca
      661 acaagtcttt ggctttttgc gcgccttctt ctttggtgaa attctgagaa tggttgtaca
      721 agagcggcaa catcacattt tgcaaagcgg ttgtgcgagc aagaaggtta taggcttgaa
      781 agatgaaacc aagtttctgg ttacgaaccg aggctaattg caccttatca agctggctta
      841 catcttcacc gtctaaaaaa tattgaccgg acgatggctt tgaaagacag ccaaggatgt
      901 gcatcagcgt acttttgcca gagcccgaag gacccataat ggctacaaat tcgccttttt
      961 gaatttggat gctcacgcct cgtaaggcag agacgctaat atcacccatg ccataaactt
     1021 tggtaatatc gacggtttca ataatcgaat gttctgtgct cattgcgtct ccacgatgcc
     1081 ggttgttacc aggtcgcctt cttttacgcc tgaaataatc tccgcataaa gcaaatcttg
     1141 aatacctact tcaaccacat gcaaaatggg tttgtcattt tccataataa aaacggcata
     1201 ttggtctcct gccttgtgca aggcttcgat gggcaccaac actgcatttt cagcccgccc
     1261 gccgataaca tcaacagaag cggttgtgcc taaaggtaaa gtgaagcttt cttcatcaat
     1321 ttgagtcaac tttacgaaag cccgaactac agaagtattg ttggaggagt acagcccagg
     1381 gtctacctgt gttacaactc cgctgaacgt tttatcaggc aaaatatcga aaataacatc
     1441 tgtctcatag cccaccacaa cgctcgacca atctgattca tctaaataca cttccaaata
     1501 tggctgggtt agatccgcga gcgtaatggc agaactgccg ctgctcacac tatctccaac
     1561 cgatatatca atggacataa ccgtacctgc atacggtgca atgatctgcg taccgtctaa
     1621 agtcgctttt gcagattcga ggttcaagcg tgcttgctca agctcggtca agccgctgcc
     1681 ggttgcattt tcaggaattt tctcgccggt caatgctgca tacagccatt tagcttcttg
     1741 tagggtcgcc ttggcaagtt catagtctgc acgtgcggca agaatctcag cctctgaagg
     1801 tgcatttaat tctttgccat ctgtgaaata tttgggaagg taataacttg tgtaacgtcc
     1861 ttgcgcctgt gccaacagtt cttgggatgc ttcaaggtca gactgagcgt cctctaaggc
     1921 ttttttcgcg tctgtgtctg tgggtgactc tttgagcttc gcctcagcct cggcgatagc
     1981 tgtttgtgct tcttcaacct tttcctccgc ctgaagcgct tctgggctaa taatataagc
     2041 tagggtgttt tttgcatcgc tgacatcact agtcgcatct gcaatggatt gttgagcagt
     2101 agcgattgcg ctgggagaag ttaactctaa ataagctcgg tttgcttccg catatgctgc
     2161 ttgggcgtct gtatcatcga gctttgccag ttgagtacct gcttccacct tttcaccaac
     2221 ggtcacatta acttcggtca ctttgccggt agacgtaaag gaaagctcgg tttcatttgc
     2281 agagactaaa gtgcctgtgc cactggcata aatgacaaga tcacctctgg ttgctatagc
     2341 aaccagaggt gatcttgtca ttt
//
LOCUS       DAOQFT010000406         2362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29323_length_2362_cov_3.559168, whole genome shotgun sequence.
ACCESSION   DAOQFT010000406 DAOQFT010000000
VERSION     DAOQFT010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29323_length_2362_cov_3.559168"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(18..223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            537..866
                     /locus_tag="PLR93_10045"
     CDS             537..866
                     /locus_tag="PLR93_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27352.1"
                     /translation="MPTLIHECQCDACQQSKHHLDWQLHHQMNVFLSRLDEQQRRWYV
                     ALESKKIGYGGDKQLAQITGMDVETIRRGRRELDEDLATRPSEAIRDAGGGQPPVEKK
                     TRRSRRR"
     gene            857..1798
                     /locus_tag="PLR93_10050"
                     /pseudo
     CDS             857..1798
                     /locus_tag="PLR93_10050"
                     /inference="COORDINATES: protein motif:HMM:NF033519.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
     gene            complement(1825..2001)
                     /locus_tag="PLR93_10055"
     CDS             complement(1825..2001)
                     /locus_tag="PLR93_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27353.1"
                     /translation="MKNEVFLDTAYAIALSVETDAHHQQAETLAETLEENQTYLVTTR
                     AVLLEIGNALSKGL"
     gene            complement(1991..2230)
                     /locus_tag="PLR93_10060"
     CDS             complement(1991..2230)
                     /locus_tag="PLR93_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF104 domain-containing protein"
                     /protein_id="HNH27354.1"
                     /translation="MTMQQTLEATFDGKVFRPVGKVKLQPDTQVQLIVTVKAEPEKKP
                     KSFLRVARSLKLDGPSDWSERVDDYFYGGAELDEK"
BASE COUNT          600 a          593 c          595 g          574 t
ORIGIN      
        1 cctcctttcc gcgagatgtg gaacacgaca aacagagcgg gcgacctggc ttgaggaagg
       61 ggaagaagga agtaggaaat aggaagtagg atttgatacc ttcttccaga ttcctgattc
      121 ctaatccctt tctcttacag ttgcgggaca gcgccggact tgaaccggct tcgccagtga
      181 aaaagatgcg aaatgctcgt tcactgcctt cccatccggg ggaagtggag ttcgcgtggg
      241 caaactctca ggctctctgc ttgtggttgt ataaggttgt agagggcatt gtaccactca
      301 gtgtccccgt cggggcgaga cgggcgggaa atgatctcga tttgcaaatt aggatactgt
      361 cccgcctgtc tcgcccttac gagccacata cacgagaggg cgaaacccgc atccttcgac
      421 gccgcaaagc ctctgctcag ggcctgtaaa agtataagtc cgcgttttgg aattttaaga
      481 tttggcttgg ggtagaatgg tatgttttag aaaccatctc aaccaggaca aacccaatgc
      541 ctacactcat tcacgaatgc caatgcgatg cctgtcaaca atccaaacat catctggact
      601 ggcaattgca ccaccagatg aacgtttttc ttagccgcct cgacgagcaa caacgccgtt
      661 ggtacgtagc gctcgaatcc aagaaaatcg gatatggcgg cgacaagcaa ctcgcccaaa
      721 ttaccggcat ggatgtcgag accatccggc gtggtcggcg agagttggat gaagacctgg
      781 caacccgtcc cagcgaagcc attcgcgatg ctggaggcgg gcagcctccg gtcgaaaaaa
      841 agacccggcg ctcaaggcgg cgttagccga cctgctgaaa gacgaaacgg ctggtgaccc
      901 gatgagcgag cagaaatggc aacgcagtag cttacgttac ctggtagata cattagctcg
      961 cctgggtcac tggatcagtc acaccacagt ggctcggcta ctcaaggaga tgaagtattc
     1021 gctcaaagcg aacgtcaaac gtctggcggg tgtcgcccat ccggacagaa accaacagtt
     1081 tgagtatatt gccaagcaga aacgcatttt tctggaggct ggtttaccga ccatcagcgt
     1141 ggataccaag aagaaagagc ggatcggcaa cttcaagaac gctggtcaaa cttggtgcca
     1201 aacgccagaa gccgtgcacg accacgattt tgatcaagag gccttgggca aagccgttcc
     1261 gtatggcatt tatgacctga agcacaaccg aggctatgtg tatgtcggca tgtcagccga
     1321 cacaccccaa ttcgctgtgg agatgattgt ccgttggtgg acaaccgaag gccaggtcct
     1381 ctacccgaat gctgacaagc tcctgatcct ggcggatgcc gggggcagca atggctgtcg
     1441 cccaagactg tggaagcaac agcttcaaga actcctggca gaccgcctag gcttggaagt
     1501 caccgtgtgt cactacccaa ctggcgcttc caaatggaac ccgattgagc atcgcctgtt
     1561 tggacccatc agcatcaatt gggctggcaa accattgcgg actttcgagt tcatgttggc
     1621 ttgtattcgt ggcacagata cggatacagg tctccaggtc aaagctttct tggtagatga
     1681 aatctaccag aaaggcattc aagttaccaa agctaccatg gaaaccttac aaatccattg
     1741 gcacgaggtt tgtccaaggt ggaattacac catcaagcca cgattgtcga atctctgaag
     1801 ttttcaaagt gcggagttat tcttttacaa gcccttagat aaggcgttac caatttcaag
     1861 taatacagcg cgtgtagtaa caagataggt ttggttttct tcgagggttt cggctaatgt
     1921 ttctgcttgt tgatgatgtg catctgtttc tacagaaagc gcgattgcat aagctgtatc
     1981 caaaaacact tcatttttca tcgagttctg cgcccccgta aaaatagtcg tccacacgtt
     2041 cagaccaatc tgagggacca tccagcttca gagaacgtgc tacgcggaga aatgattttg
     2101 gctttttctc tggttcagcc tttacagtga caattaattg aacttgggta tctggttgta
     2161 attttacttt tccaaccggg cggaagactt ttccgtcaaa ggtggcttct agggtttgtt
     2221 gcatcgtcat cttcttctcc gtttattttg gatttagagg cttttgaagg ctatcttcat
     2281 tataccaatc cattcacttg agcaaagacc acgctcattc tcgccggtaa atacctcgca
     2341 aatctttctc ccttcattcc ct
//
LOCUS       DAOQFT010000407         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29340_length_2361_cov_4.633998, whole genome shotgun sequence.
ACCESSION   DAOQFT010000407 DAOQFT010000000
VERSION     DAOQFT010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29340_length_2361_cov_4.633998"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1416
                     /locus_tag="PLR93_10065"
     CDS             124..1416
                     /locus_tag="PLR93_10065"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNH27355.1"
                     /translation="MEEEKKEIVSEEDQPNLVKMEVNFLQYPMGTLDKRSTLKEITYK
                     DTIPTPGGKVEIEWQVIGSAKYGIPGPTALELDDAITQMLMQKERWKNGRPPKWMATT
                     YYELSETMGMKPSGRVYKMMKRDIERIRTTTNISKGAYKTRDRSGGRSWFDGQVNKYQ
                     FLGQLGENPDKCIPDEIVQTEGETKVIIGLTDIYWENIAGIAGKPYTMPINFTFMRTL
                     ESSVLKRVYKLLALAFQTMERTEYKGEECINYNYQELCGRTPIKTWEERYKAKGHMQR
                     YLEPFVTNGILRKYTLEFEKEDRTRWFVRLYQGPRAKEDEQRILGQIDLFERIEPKLL
                     ETDQQRRITENRNAKKKNKKVAEQENEMREAEARQKSDELTRYYNGLNDEGKKVVDEV
                     IRAKIEMYPPMVRNSKLTADVARADAIEEYKMRQDANR"
     gene            1475..>2361
                     /locus_tag="PLR93_10070"
     CDS             1475..>2361
                     /locus_tag="PLR93_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH27356.1"
                     /translation="MSLQQILKGILPRQNQIKAEVDGTFEIRDGELIVKGGTGTPDVG
                     GASEPDRRNEELKGFYVALEAAGRSRLTLSGYASDMRFWERQGAVWDKQIRDLKVGEI
                     EPGIVGLDVATRKRKVAAIRAYARHLLRSGSADLLLELEKLNRPRPKGRIARVTNDLE
                     YKETREKARELCGKADRRGIWLGLMTWCGLRISEIATAQAGDGWIQVIGKGNKERRVP
                     CPDWLCDAVKNAKKKEKGGYQRARRIIDRSLRKDGYSHFHKFRHTYATTLLHRGVTLD
                     QIRVLLGHASINTTQIYAKT"
BASE COUNT          790 a          442 c          681 g          448 t
ORIGIN      
        1 gcttatgagg tatgaagtat tggcttatga ggtatgggcg tccgacggta aaaagtgcat
       61 tcagtataag ccagtagaag acagcaagcg gctacgccgc tttttttgct caggaggtca
      121 aaagtggaag aagaaaaaaa agaaatcgtg agtgaggaag atcaaccgaa ccttgtaaag
      181 atggaagtca acttcttgca atacccaatg ggaacgcttg ataaaaggtc aacgctgaag
      241 gaaatcactt acaaagacac aatcccaaca cctggcggga aagtggaaat tgagtggcaa
      301 gtcattggat cagcgaaata tgggataccg ggcccaaccg cgcttgaact cgatgacgca
      361 atcactcaaa tgttaatgca gaaagagcgg tggaaaaatg ggcgccctcc caaatggatg
      421 gcgacaacct attacgagct atcggaaacg atgggaatga agccatcggg acgagtctac
      481 aagatgatga agcgagacat tgagcggata agaacgacga ccaacatatc caaaggggca
      541 tacaaaacac gtgaccgaag tggtggaaga agctggttcg atgggcaggt gaataaatac
      601 cagttccttg gacagttagg tgaaaaccca gacaaatgta tccctgatga gatcgttcaa
      661 acagaagggg aaacaaaagt cattattgga ctgacggata tttattggga aaacattgcg
      721 gggatagcgg gaaaaccgta cacaatgcca atcaatttca cgtttatgag gacattggaa
      781 agcagtgtct tgaaacgggt ctataagctt ttggctttgg cgtttcagac aatggaacga
      841 actgaatata aaggggaaga gtgcatcaac tacaactatc aagaactatg tggcagaacc
      901 ccgatcaaga cgtgggagga gaggtataaa gcgaaagggc acatgcaacg gtatcttgaa
      961 ccattcgtaa ccaatgggat tctgcggaaa tacacgcttg aatttgagaa ggaggacaga
     1021 acacggtggt ttgtccgact ctaccaggga ccgagagcaa aggaagacga gcaaagaata
     1081 ttagggcaaa tagacttgtt cgaacgaatt gaaccaaagt tacttgaaac tgaccagcag
     1141 aggaggatca cagaaaatag aaacgccaag aagaaaaaca aaaaggtggc agaacaagaa
     1201 aacgagatga gagaagctga agcacgccaa aagagcgatg agttaacccg gtattataat
     1261 gggctgaatg atgagggaaa gaaagtggtt gatgaagtga tccgcgccaa aatcgagatg
     1321 tatccgccga tggtgaggaa cagcaagctg accgccgatg ttgcaagagc tgatgctatt
     1381 gaagaataca agatgaggca agacgctaac agatgaacag aagaaagata cagtggtatt
     1441 gatataaatt ggcggaaata atatacaggt aggcatgagc ctacaacaaa tattgaaggg
     1501 tatacttccc cgccagaatc aaataaaagc ggaagtagac ggaacttttg aaataaggga
     1561 tggggagcta attgtaaagg gcggaacggg cacccctgat gtgggtgggg caagcgaacc
     1621 ggaccgcagg aatgaagagt taaaggggtt ttacgttgcg ctggaggctg ctggccgaag
     1681 tagactaacg ctatcagggt atgcgtctga tatgcgcttc tgggagcggc agggagcggt
     1741 ctgggacaaa cagataaggg acctaaaggt tggagaaatc gaacccggga tagttggcct
     1801 tgatgtcgcc acacgaaaac gcaaggtggc cgcaataaga gcgtatgcgc gccacctgct
     1861 ccggtccgga agtgctgacc tactgctaga acttgagaaa ttgaaccggc caaggccgaa
     1921 aggccggatt gccagggtta caaatgacct tgagtacaaa gaaacgcgag aaaaggcccg
     1981 cgagctgtgc gggaaagcag accgccgcgg gatatggttg gggttgatga cctggtgcgg
     2041 gttgcgaata tctgaaattg caaccgcaca ggcgggggat ggatggatac aggtgattgg
     2101 gaagggcaat aaagaaaggc gcgttccctg ccctgattgg ttatgtgatg cggtaaaaaa
     2161 tgcaaaaaaa aaggagaagg gcggatacca gcgggcaagg cgaattattg ataggtcgtt
     2221 gaggaaggat ggatacagcc acttccacaa atttcgacac acatacgcga caacgttgct
     2281 tcatcgtggg gtgacactcg atcaaattcg ggttcttctt ggccatgcga gtattaacac
     2341 gacccaaatc tatgcaaaaa c
//
LOCUS       DAOQFT010000408         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29396_length_2357_cov_5.639878, whole genome shotgun sequence.
ACCESSION   DAOQFT010000408 DAOQFT010000000
VERSION     DAOQFT010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29396_length_2357_cov_5.639878"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>2357
                     /locus_tag="PLR93_10075"
     CDS             36..>2357
                     /locus_tag="PLR93_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH27357.1"
                     /translation="MTLLGLRCDSYIALGKFGLAEADANAMRELADPSDPELEIRALK
                     HQASVMLVKGELVEAAQTAGQAVAQARALKPPNPYLLAQCLYMLGDCHKNSGNYENAI
                     VAAKEAIPLFEATGSVKETGRTHWLMAPSYNVLGNAKKSREAAQNALNLCQQAGDSMG
                     VGNAYNALSSTDTDIAERIQHYQKAIQAFEISGYLERVTGAIGNLGFTYAALGLYMHS
                     CRLTNKALEIDRSMGAKLGMSYWLENLFDAELRLGGGDLARQHLNEFTALTPDLGSSR
                     VTVSQHMNFGLMALGEEKPAEAVEHFQTAVELARESNAGNLNVALTYLSHAHLVHKNP
                     VAALEASTQATELHRQQGYKLPDSFSSQEIWWRHTQALLANGKTTAARAALERAYKFL
                     LEAITNVRDEGLRRNYLNKVQENRDLLNFWVKDSLKHKLTRKRIFAHLFIESNTREPF
                     KRLTDTSLRLNALHAVEDIHTFIVEEATELSGGERVLFIQDKDGTYEVAGAYLPRGEQ
                     PQDVLKGIETYLTEARLTRTVQLIVPGELDPAPKRSTRQPQSVMRGERLSRIIAPLIA
                     QNQVVGHIYADMDSLYGAFTEVDSAMLGMLASQAAVTLDNVHWAQGLELKVQERTAEL
                     RARVDELAILNRVQAGLASQLDVNAIYELIGEQLRSLFDSQAISIVSFDVERDIRHYH
                     YILEQGQRLEVADAPIPPISKYIIQHKQPLLINDHFKERIAEIGLKSNTLPGTKPTKS
                     LLRMPILVDDEVKGVIGLDNIDHENAFNDTDVRL"
BASE COUNT          621 a          589 c          622 g          525 t
ORIGIN      
        1 aaaaattaag ctcagaatct gatgtcgatt taagaatgac cctgctgggt ctgcgatgtg
       61 actcatatat cgcccttgga aaattcggtc tcgcagaagc tgatgcaaac gccatgcgtg
      121 agttggcgga cccaagcgac cctgagttgg agatccgcgc gcttaagcat caagctagtg
      181 tgatgttagt caaaggcgag ctggttgaag ccgctcaaac tgcaggacag gcagtggcac
      241 aagcacgtgc cttgaaaccg cctaacccgt atcttctagc acagtgcctg tatatgctgg
      301 gagattgcca taaaaattcg gggaattatg aaaacgccat tgtcgcagca aaggaagcca
      361 ttccgctgtt cgaagcgaca ggcagtgtaa aagagactgg tcgcacgcat tggttgatgg
      421 ctccttctta taatgtattg gggaatgcga agaagtcgcg cgaggcagca caaaatgcat
      481 tgaacctttg ccaacaagcc ggcgactcga tgggggttgg aaatgcctac aatgcgctca
      541 gttccaccga taccgatatt gcagagagaa tacaacacta tcaaaaagcc attcaagcct
      601 ttgagatcag cggatatttg gaacgcgtga ccggcgcaat cggaaacctt gggtttacct
      661 atgcagcgtt gggcttatat atgcattcct gccgcctcac caacaaagcg cttgaaatcg
      721 atagaagcat gggcgcgaaa ttgggcatgt cgtactggct cgaaaatcta ttcgatgcag
      781 aattacggct gggggggggg gacctagctc gacagcacct gaacgagttc acggctctta
      841 cccctgattt aggcagttcg cgcgttaccg ttagtcagca tatgaacttt gggttgatgg
      901 ctctcggcga ggaaaagcct gctgaagcag tggagcactt tcaaaccgca gtggagttgg
      961 cacgcgaatc aaatgccggc aacttgaatg tggcactcac ctacctttct catgcccacc
     1021 ttgtacacaa gaacccagtt gcagcattag aggcatcgac tcaggcaacc gaattgcacc
     1081 gccagcaagg ctataaactg cccgatagct tcagctcaca agagatctgg tggcgacaca
     1141 cacaggcact attggcaaat ggaaaaacta ctgctgcccg tgctgccttg gaacgtgcct
     1201 acaaattcct gctggaagcc attacgaacg tacgggatga aggattgcgg cgcaattatc
     1261 tcaacaaggt acaagagaac cgcgacctgc tcaatttctg ggtgaaagac agcctcaaac
     1321 acaaactgac gcgcaaacgc atttttgccc atctcttcat tgaatccaat acccgcgagc
     1381 cgttcaaacg cctaaccgac accagcctac gcctaaacgc tttgcatgca gttgaagata
     1441 ttcatacttt cattgtggaa gaagccaccg agctgagcgg cggtgaacgg gtgctattta
     1501 ttcaagataa agatgggacg tatgaagtag ccggagccta tctgccgcgc ggagaacaac
     1561 cacaggatgt gttgaaaggc atcgaaacct atctgacaga agcccgcctc acacggacgg
     1621 tgcagttgat cgtgcccgga gagttggacc ccgccccgaa acgctcgacc cgccaacctc
     1681 aatccgtgat gcgtggggag cgtctctctc ggattattgc gccgctgatc gctcaaaacc
     1741 aggtcgtcgg tcatatctat gccgatatgg attcgctgta tggtgcattc acagaagtgg
     1801 atagtgccat gctcggcatg ttggcaagcc aggcggctgt cacattggat aatgttcact
     1861 gggcacaagg actcgagcta aaggtccaag agagaaccgc ggagttgcgg gcgcgggtgg
     1921 atgagcttgc catcctcaat cgggtgcagg cggggttggc gtcacaactc gatgtgaatg
     1981 ccatttatga attgatcggt gaacagttgc gcagtctctt tgattcgcag gcgatctcga
     2041 tcgtttcgtt tgatgtggag cgcgatatac gccactatca ctacattttg gagcaaggtc
     2101 agcgcctcga agtggcagat gcgcccatcc cgccgatctc gaaatacatc attcaacata
     2161 aacagccgtt gctcatcaat gatcatttta aagaacggat tgccgagatc gggttgaagt
     2221 cgaataccct gccgggcacc aaacctacca agtctttact gcgcatgccc attttggtgg
     2281 atgatgaagt aaagggtgtc attgggctcg ataacattga tcatgaaaat gccttcaacg
     2341 ataccgatgt acgcttg
//
LOCUS       DAOQFT010000409         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29427_length_2356_cov_3.281617, whole genome shotgun sequence.
ACCESSION   DAOQFT010000409 DAOQFT010000000
VERSION     DAOQFT010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29427_length_2356_cov_3.281617"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2356
                     /locus_tag="PLR93_10080"
     CDS             <1..>2356
                     /locus_tag="PLR93_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH27358.1"
                     /translation="HIISVNLFASHRAEVEQFRLRQLNLVREVSAQIANVLNVDDLAS
                     QVTRLIQKTFNFYYVAFFTLDPQDNALHFRASAVAPRKGRRKASIALEVEIGQGLIGE
                     AAKTGMEIVCDDVQADARYRFIDSLPETRSEVVIPLKIEDHILGALDVQSNQPSAFHP
                     IDLLILRALADNVARAVDGAKLYSALSRRANQLTLVSEVSKSVTSTLDLSQLMRDAAS
                     LIHEKFGYPHVSLFTVHPNRRMIMYEAGSGKRSKKLEGFTIPLDDPIGIMPWVARQGK
                     TVLANDVSKDKRYVQSPLPPKNTKSELCVPLIFNERVVGLLDIQSDQLNAFTEDDRVM
                     FEAVADTMAAAIRNADLYRSEQWRRQVADSLRDVAGLVSSNAGLDEVLEMILAELDRN
                     LPIDVSAIWLLQEGELCLSAVHGIDGAQLENTCIAFPESTYLMTEALESDVPIIRRPE
                     DPMGPAGLTMNFDPNYSSIVAPLRVGDRPLGVLALAHRTSGRYGHEARAIAGTFANYA
                     AVAMENTRLFDAAQEQAYASAALLQVAQAVVSLNDLDEILGSIIRIMPILVGVKRVVL
                     YRLDMEYQTLQASQEYGFSEEEEAIMTEKILPVADFPFLVWALEQNTLVTYPLKAEVT
                     PLDWFAIQPGAENEAHGMDADRLLIAVPLSIKHDIFGVMLIEEDENARRFRSRRLEII
                     TGIAQQAALAIQNDLLQQEMVVRERLETEVQLARQIQQTFIPSILPTHPNWQIAARWR
                     TARQVGGDFYDVIELPNNRLGIFIADVADKGMPAALFMALTRTL"
BASE COUNT          562 a          553 c          600 g          641 t
ORIGIN      
        1 gtcatatcat ctctgtaaat ttattcgcct cgcatcgcgc cgaagtggaa cagtttcgtc
       61 tgcgccaatt aaatttggtg cgcgaggtca gcgctcaaat cgccaacgta ctcaatgtgg
      121 acgatttggc atctcaagtg acacggctta tccaaaaaac gtttaacttt tattacgttg
      181 cttttttcac cctcgaccct caagataatg cgcttcattt ccgtgccagt gccgttgcgc
      241 cgcgtaaagg tcgccgtaaa gcttccattg cattagaggt ggaaatcggt caggggctga
      301 tcggtgaagc cgcgaaaacc ggtatggaga tcgtctgcga cgacgttcag gcagatgctc
      361 gttatcgttt tattgatagc ctccccgaga cacgctctga agttgtcatt ccgctcaaga
      421 tagaagacca tattctgggc gcattggatg ttcaaagtaa tcagccgagc gcttttcacc
      481 ctattgactt actcatctta cgagcccttg ccgacaatgt tgcccgtgcc gtagatggag
      541 ccaaacttta tagtgccctg agccgacgtg ccaatcaatt gaccttggtt tcagaagtca
      601 gtaaaagcgt tacatctacc ctcgatcttt ctcaactaat gcgtgacgca gctagcttga
      661 ttcacgaaaa atttggctac cctcacgttt cgcttttcac cgtccatccg aatcgacgca
      721 tgatcatgta tgaagcaggg agcgggaagc gcagtaaaaa acttgaaggc tttacgatcc
      781 cactagacga cccaattggc attatgcctt gggtggcacg tcagggaaaa acagtgcttg
      841 cgaatgacgt ttcaaaagat aagcgttacg ttcaatcgcc attgccgcct aaaaacacaa
      901 aatcagagtt atgtgttcct ttgatattta atgagcgtgt ggttggtttg ttggatatcc
      961 aatcagacca actcaatgct tttactgagg acgaccgggt catgttcgaa gcagtggcag
     1021 acaccatggc tgctgctatt cggaatgcag atttatatcg ttctgagcaa tggcggcgcc
     1081 aagtagcaga cagtcttcgt gatgtagcag gcttggtttc aagcaatgcc ggtttggacg
     1141 aagtgctcga aatgatcctt gccgagttag atcgtaacct tccgatagac gtttctgcta
     1201 tctggctatt gcaagaaggc gagctatgtc tttcggctgt acatggtatt gatggcgccc
     1261 aactcgagaa tacttgtatt gcatttcctg agtccacgta tttgatgacg gaggcactcg
     1321 agtcggatgt ccctatcatt cggcgccccg aagatcctat gggtcctgcc gggttgacaa
     1381 tgaactttga tcccaattat tcttctatcg ttgctccgtt gcgagtagga gaccgtccat
     1441 tgggtgtctt ggcgttggct catcgtacgt cagggcgtta tgggcacgaa gcgcgtgcca
     1501 ttgcagggac ttttgcaaat tatgcagcag ttgcgatgga aaacacccgt ctctttgatg
     1561 ctgctcaaga acaagcctac gcctcggcgg cattattgca ggtggcacaa gccgttgtca
     1621 gtctgaacga cttggacgag attctcggct cgatcatccg tatcatgccg attttggtag
     1681 gtgtcaaacg agttgtgctc tatcgtctcg atatggaata tcaaaccttg caagcatcac
     1741 aggaatatgg cttttctgaa gaagaggaag ccatcatgac tgaaaagatt ttgcctgtgg
     1801 ctgattttcc cttcctggta tgggcattgg agcaaaatac tttggtgacc tatccgctta
     1861 aggctgaggt gacgccattg gattggtttg cgattcagcc tggggcggag aatgaagcgc
     1921 atggcatgga tgcagaccgt ttattgatcg cggttccatt atccattaag cacgatattt
     1981 ttggcgtcat gttgatcgaa gaagatgaga atgcgcgccg tttccggtca cgccgtttgg
     2041 agatcatcac tgggattgct caacaggcag cgcttgcgat tcagaatgat ttgcttcaac
     2101 aagagatggt ggtgcgtgaa cgcctcgaaa cggaagttca gttggctcgc caaattcagc
     2161 agaccttcat cccaagcatt ttgccaaccc atcccaattg gcagattgcg gcaagatggc
     2221 gcacagcacg gcaagttggc ggtgacttct acgatgtgat cgagttgccg aataataggc
     2281 ttggtatctt cattgctgat gtcgcagaca aaggcatgcc tgctgcattg ttcatggcgt
     2341 tgacgcgtac gttaat
//
LOCUS       DAOQFT010000410         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29439_length_2355_cov_5.576522, whole genome shotgun sequence.
ACCESSION   DAOQFT010000410 DAOQFT010000000
VERSION     DAOQFT010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29439_length_2355_cov_5.576522"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1232
                     /locus_tag="PLR93_10085"
     CDS             303..1232
                     /locus_tag="PLR93_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27359.1"
                     /translation="MVRKLSVVLLILFQISCSAATPEPTATFTPLPPTATVTPTPAPT
                     ATQLPASPMPDAITYEGNGFRFAVPDTLDVDMQKNAVGIYDKSGTLIISFTRVPYDES
                     TDTLEDVINDYLAEISTRGGGKFTQSEATSITVAGAEGLSVELSGTLFERPIEGRAIA
                     VVPDKNSVLFGMGVSNLVSDETLWQSTGSLIFQGLLDSLEFVDVQIAAQCTVSTDKSY
                     GYTETNPIKVGGDFIDGPARERAYLDNLLGPNGEALSYEREGSINSGDTILDIYRVTG
                     DGLNITLYLDEYTYSPLLAPVGLTCVNEFISAP"
     gene            complement(1277..>2355)
                     /locus_tag="PLR93_10090"
     CDS             complement(1277..>2355)
                     /locus_tag="PLR93_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH27360.1"
                     /translation="KNNIAVFMGAAQFKAKDTLAVTDKDGKVTELKAKNIIVATGASA
                     ATLPGVTIDGKKIVTYTEAIMQDTLPQSAVIVGAGAIGVEFATIWNSYGVDVTIVELL
                     PRVLPREDEEISKELTKELTKNGIKIKTGIKFESITVSGNKVKVVLPDGTLEVDQVLV
                     ATSFTPNSKGLGLEAVGVKISDRGFIEINEKMQTNVPSIWAIGDVTGKLMLAHVGSAM
                     GIVAAEHIGGHETITLNYEMMPRATYCHPQVASFGLTEAQAKERGYNIKVGRFPFQAN
                     GKALGLGDYAGFVKIITDEKYGEILGAHMMGPEVTELLPELTLAQMMELTPHEIARNV
                     HAHPTLSETLMEAAHGASGTPIHI"
BASE COUNT          529 a          568 c          594 g          664 t
ORIGIN      
        1 cgatgaccac tgcccattgc ggaccgataa tgacacccgc agtgacctgc gcgtagactt
       61 cgctttggaa ccaaaatacg ttttcagaaa ctcgttcgcg gtgcatagtt ttaccttctt
      121 cactggttcg ccggtttgct atcccatact ggggcgaagc catttgtgtt tagtattcgt
      181 tgttaatagc atacaatctt aaaaatttca agatgtatag acaaatatgg agatttcttc
      241 agatttgagt tatattctga caagacttgt gcctgtccct tcacccaaaa ggaggattct
      301 agatggttcg aaaactttct gttgttttgt tgatcctgtt ccaaatctca tgtagtgcgg
      361 ctacccccga gccaacggcg acttttaccc cattgcctcc cactgctaca gtcacgccta
      421 cgcctgctcc aactgctaca cagcttccgg ctagccctat gcctgatgcg attacatacg
      481 aaggcaatgg gtttcgcttt gcggtgcctg ataccttaga tgtggatatg caaaagaatg
      541 ccgtggggat ttatgataaa tctggcactt tgatcatctc ttttacccga gtgccgtatg
      601 atgaatctac cgatacattg gaagatgtga tcaatgatta tctggcagag atttctacgc
      661 gtggtggcgg caagttcacc caaagcgagg cgacttcgat cacagtggct ggggcagagg
      721 ggctttctgt ggaactaagc ggcactcttt ttgagagacc cattgaaggc agagccattg
      781 cggtggtgcc tgacaagaat tcggtgctat ttggtatggg agtctcaaat ttagtcagtg
      841 atgagacgct atggcaaagc actggttcat tgatctttca ggggctattg gattcactgg
      901 aatttgtcga tgtgcaaatc gctgcgcagt gtactgtcag cacagataaa agctatgggt
      961 atacagagac caacccgatc aaggtcggag gcgattttat cgatggacct gctcgtgaac
     1021 gagcctatct cgataatttg ttgggtccga atggtgaggc tttgtcttat gaacgtgaag
     1081 gctctataaa tagcggtgac acgatcttag atatttatcg tgtaacaggc gacggtttga
     1141 atataacctt gtatttggat gaatacacat attcgcctct ccttgcaccg gtggggttga
     1201 cctgcgtgaa tgagtttatc tctgccccgt aatgtgaata aaaaactccg aagcttcatc
     1261 gcttcggagt tttgttttag atgtgaatgg gagttccgct tgcaccatgt gcggcttcca
     1321 tgagtgtttc tgaaagcgtt gggtgcgcat gaacgttacg agcaatctcg tgcggagtca
     1381 gttccatcat ttgcgcgagg gtcagttcgg gcaacagttc ggtcacttcg ggtcccatca
     1441 tatgagcacc gaggatctcg ccatattttt catcggtgat gatcttgacg aagcctgcat
     1501 aatcacccag cccaagtgcc ttgccatttg cctggaaggg gaagcgccca accttgatgt
     1561 tgtatccgcg ctcttttgct tgcgcttcgg tcagcccaaa cgaagcgacc tgcggatgac
     1621 agtaggtagc acgcggcatc atctcgtaat tgagggtgat ggtttcatgc ccgccaatgt
     1681 gttcagcagc cacaatgccc atcgctgagc cgacatgtgc cagcatgagt ttgccggtca
     1741 cgtcgccaat tgcccaaatg ctgggaacgt tggtctgcat cttttcattg atctcaatga
     1801 agccacggtc gctgatcttg acaccaaccg cctcaagccc cagccctttg ctgttcggcg
     1861 taaaggaagt tgcgaccaag acctggtcca cttctaacgt tccatctggc aacacaactt
     1921 tcactttgtt cccgctgacg gtgatgcttt caaacttgat gccagttttg atcttgatgc
     1981 cgttcttggt cagctctttg gtgagttcct tgctgatttc ttcatcttca cgtggtaaca
     2041 cacgcggaag cagttccacg atggtcacgt ccacgccgta ggaattccaa atggtggcaa
     2101 attctacgcc gatggcaccc gcacccacga tcaccgcaga ctgggggagg gtatcttgca
     2161 taatagcttc agtataggtg acaattttct tgccgtcgat ggtcacaccg gggagggtgg
     2221 ctgcgctggc gccggtagca acgatgatgt tcttcgcttt aagctcggtg actttgccgt
     2281 ctttatctgt cacggcaagg gtatctttgg ctttgaattg tgccgcgccc atgaagacag
     2341 caatattgtt cttct
//
LOCUS       DAOQFT010000411         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29470_length_2354_cov_8.000870, whole genome shotgun sequence.
ACCESSION   DAOQFT010000411 DAOQFT010000000
VERSION     DAOQFT010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29470_length_2354_cov_8.000870"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLR93_10095"
     CDS             complement(<1..239)
                     /locus_tag="PLR93_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNH27361.1"
                     /translation="MKYTKICVIGLGYIGLPTASTFAMNGIEVLGVDISPTIIETLNR
                     GEIHIHEPGLSEAFTNAVKSGKFKAALKPEEADAF"
     gene            369..965
                     /locus_tag="PLR93_10100"
     CDS             369..965
                     /locus_tag="PLR93_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNH27362.1"
                     /translation="MDFLLDPNIAYLFLLGGVVLAFLAIVTPGTGMLEVGAIFCFLLA
                     GYAVYNLPMNWWALLILLLSVVPFLYAVQKPKRELFLGVSIFLLVLGSVFLFAVDGWK
                     PAVNPLVALFSSGVVAAFLWIVVRKSIQAADARPTHDLGILVGQMGEARTAVHQEGSV
                     YINGELWSAKSVSEIPAGSHVLVVAREGFVLVVEPIKS"
     gene            983..1891
                     /locus_tag="PLR93_10105"
     CDS             983..1891
                     /locus_tag="PLR93_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH27363.1"
                     /translation="MNGAFASFGLCLVGFIVLVIVIAAFNMIRVVPEYQRLVVFRLGR
                     VLEKPKGPGLVFLIPGVDRPVKVDLREQVREVPHQTSITKDNAPISIDFLWYYKVLDP
                     AQSVLQVGNFESAAAGIAATTLRAVIGGIPLDDVLSEREHINNMLRTRLDEVTERWGV
                     KVTNVEIREIIPPRDVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQSNILR
                     AEGQKQSAILQAEGERQAQLLRAQGFAQALETIFGAANKIDQKTMTLQYFETLKSMGM
                     SPSTKYIFPMEFTSMLDNFLGKDSKK"
     gene            1942..>2354
                     /locus_tag="PLR93_10110"
     CDS             1942..>2354
                     /locus_tag="PLR93_10110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH27364.1"
                     /translation="MEIIPANILDLTALRKLEKESFDRDAWPMIDLIAVLTFSNVVRL
                     KAMENGNMIGFVAGDPRPRDGWGWIATIAVDSNFRRRGIGTALLHACESQLGVQRSRL
                     TVRTSNQGAINLYEKEGYRTIDVWKAYYSDGEDAIV"
BASE COUNT          564 a          473 c          605 g          712 t
ORIGIN      
        1 ataaaagcat cggcttcttc tggtttgaga gccgctttga attttccgct tttgacagcg
       61 tttgtaaatg cttctgaaag acccggttcg tgaatgtgga tctcgccgcg gttcaatgtt
      121 tcaataatgg tggggctaat gtctacgccg agaacttcga tgccattcat tgcgaatgta
      181 gatgctgtgg gtagaccaat gtatcccaac ccgataacac atatttttgt gtacttcact
      241 tgagctccta tgggagtaat tatcctgtcc attatacaac tgctagagaa attggtttga
      301 aacgtttcaa tgggcattac gtaaatcttt ataggaatgc tgttgtataa ttatatggag
      361 gcataaacat ggatttcctt ttagacccta atattgccta tttgttcctt cttggcggag
      421 ttgtattagc atttttggct attgttacgc ctggaaccgg aatgttagaa gtgggagcga
      481 ttttttgttt cttgctggct ggttatgctg tctataatct ccctatgaat tggtgggcat
      541 tgctcatttt acttttgagt gttgtgcctt tcctttatgc agttcaaaag ccaaagcgag
      601 agttattcct tggagtttca attttcctgt tggtgctcgg ctctgtgttt ttgtttgctg
      661 tagatggttg gaagccggca gtgaacccat tggttgcttt gttttcgtca ggtgttgtag
      721 ctgcgtttct ttggattgtc gttagaaagt ccattcaagc ggcagacgct cgaccaacgc
      781 atgatttggg tattttggtg ggacagatgg gggaggcaag aactgctgta catcaagaag
      841 gaagcgtgta tattaatggg gaattgtggt cggcaaagag tgttagtgag attcctgctg
      901 gaagccacgt tctagttgtt gctcgcgagg ggttcgtcct cgttgttgaa cctatcaaat
      961 cgtaaatttt gaaggagaga atatgaatgg tgcatttgca agttttggtt tgtgtttggt
     1021 cggttttatc gttctagtga ttgtgattgc cgcgttcaat atgattcggg ttgtgccaga
     1081 atatcaacgt ttggtcgtgt tccgtttggg acgtgtgctg gagaagccca agggaccagg
     1141 cttggttttc ttaatccccg gtgtggatcg ccctgtaaaa gttgatttgc gcgaacaggt
     1201 tcgtgaagtt cctcatcaga cttcgatcac aaaagataac gcgccaattt caattgactt
     1261 cttgtggtat tacaaggtac ttgaccctgc ccagtctgta ttgcaggttg gtaacttcga
     1321 gtctgctgcg gctggtattg ctgccactac tttgcgtgct gtcatcggtg gtatcccatt
     1381 ggatgatgtg ctctctgaac gtgaacatat caataatatg ctccgcaccc gtcttgatga
     1441 agtgaccgaa cgctggggtg tgaaagtaac caatgttgaa atccgcgaaa tcattcctcc
     1501 gcgtgatgtg caagacgcca tgaaccgcca gatgtctgca gaacgtattc gccgtgctgt
     1561 tgtgaccgag tctactggta cgcgtgaagc tgcagtcaac gtggcagaag gtgaaaagca
     1621 gtctaacatt ttgcgagcag aaggtcagaa acagtctgca attcttcaag cagaaggtga
     1681 acgacaggct caattgcttc gcgcacaggg ttttgctcag gcgttggaaa ccatttttgg
     1741 cgctgccaat aagatcgacc agaaaactat gaccctccaa tacttcgaaa ccttgaagtc
     1801 aatggggatg agtccttcca cgaagtacat cttcccgatg gaatttacca gtatgctcga
     1861 taacttcctc ggtaaagact cgaagaagta aaacgatttg taatacaata aggtctctcg
     1921 aaaagagaga ccttattttt tatggaaatc atccctgcca atattcttga cctcaccgct
     1981 ctgcgaaaat tagagaagga aagttttgac cgcgatgcat ggccaatgat tgacctgatc
     2041 gccgttttga cattctccaa tgtggtgcgc ctcaaagcta tggaaaatgg caacatgatc
     2101 ggttttgtcg ctggcgatcc gcgtccacgc gacgggtggg gctggatcgc caccattgca
     2161 gtggattcaa actttcggcg ccgcggaatt ggaactgcat tgctgcacgc ttgtgaaagc
     2221 cagcttggtg tccaaagatc gcgactgaca gttcgaacgt cgaatcaagg ggctatcaat
     2281 ttatatgaga aagagggata tcgtacgatc gatgtctgga aagcatacta ttctgatggc
     2341 gaagatgcca ttgt
//
LOCUS       DAOQFT010000412         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29471_length_2354_cov_7.480209, whole genome shotgun sequence.
ACCESSION   DAOQFT010000412 DAOQFT010000000
VERSION     DAOQFT010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29471_length_2354_cov_7.480209"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLR93_10115"
     CDS             complement(<1..203)
                     /locus_tag="PLR93_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27365.1"
                     /translation="MSDIVQPPKPVTEIWKPELVRLPELTSLRLAFRRFAYGLVKLVV
                     WSCLRVTREGLENIPSSGPLLIV"
     gene            complement(207..887)
                     /gene="cmk"
                     /locus_tag="PLR93_10120"
     CDS             complement(207..887)
                     /gene="cmk"
                     /locus_tag="PLR93_10120"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNH27366.1"
                     /translation="MTKTPPSIIALDGPAASGKSTVGRRLADALGYLFFDTGVMYRAV
                     TWIALNHDMDLKNEAAITQMAESAELDIRPPSQNDGRACDIIVGDKDITWDVRSGDVE
                     ASVSLVAAYAGVRTALSGQQRRIGLRGKVVMVGRDIGTVVLPEADLKVYMDASAEERA
                     RRRFKDITSRGEQANYDELLKKMIERDRLDSTRAVAPLRPADDALVLDTDKLSEEEVF
                     AKILEMCK"
     gene            complement(954..>2354)
                     /locus_tag="PLR93_10125"
     CDS             complement(954..>2354)
                     /locus_tag="PLR93_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27367.1"
                     /translation="FFLALDPGLVAISRQITSPILAITFLAFTVGFINKNKWTWAAIT
                     AALALLSGPSAWLGLLGIGITYGIAKLLARRTEESSEEENTPTEKRKRDRSSSAFGKE
                     FFKSSNLITFALAFIFAGSLFFIVPGGIGAAFSSIPAFFKTWAATGPVTQGMLIISLF
                     VYQPFALLLAGMALLRGWMGGLRRIVFLSIWFFVALLLVIFLSGRQMYDLAWALIPLN
                     ALAALELVRHLSIFPEERREVAGVVLLTTFIWVFIWLGTAGFSWILVDTPDYRLRAGM
                     LVGSIFLLIISLVLVAAGWSIRIARLGGVWGMAICLGLLSVGGLFGATGLRGLSAPEL
                     WWLPNVPTQAQLVRDTVHQVSDIGRGSIYSATVAIIGVNSPALEWAVRENPVQVVDAV
                     DPNNTPDFVITSYELDPGLVSAYRGQDFTWRQAPIWNGADTFAWLKWISLRQMPQSQE
                     AIILWARTDLFLGGTQ"
BASE COUNT          655 a          630 c          587 g          482 t
ORIGIN      
        1 ataacgatca gcaaaggtcc gcttgacggg atattttcca acccttcccg tgtgacgcgc
       61 aggcaactcc atacaaccag tttgaccaat ccatatgcga acctgcgaaa ggcgaggcgc
      121 agagatgtca actcgggcag ccgcactaat tccggcttcc aaatttcagt gacgggttta
      181 ggcggctgca caatatcaga catgctttat ttgcacatct ctaaaatctt agcaaagact
      241 tcttcttccg acaatttatc cgtatccaat accagcgcat cgtcggcagg gcgcagcgga
      301 gcgacggcgc gcgtggagtc taagcggtca cgctcaatca tctttttgag aagctcgtca
      361 tagttagctt gttcgccacg ggaagtaata tctttaaaac ggcggcgagc acgttcttca
      421 gcgctggcat ccatatacac tttcagatcc gcttcgggta aaacgaccgt gccaatatct
      481 ctgccgacca tcaccacctt accacgcaac ccaatgcggc gttgttgacc agagagcgca
      541 gtgcgcaccc ctgcatatgc cgccactaat gagacactag cctccacatc tcccgaacgg
      601 acgtcccacg taatgtcttt atcgcccacg ataatatcgc aggcacgacc gtcgttttga
      661 gagggcgggc gaatatcaag ctcggcagac tctgccattt gggtaatggc agcttcgttt
      721 tttaggtcca tatcgtgatt gagcgcgatc cacgtcacag cgcgatacat cacacctgta
      781 tcaaagaata aataacccaa tgcatctgcg agccgccgcc ccactgtaga ttttccagag
      841 gcggctggtc cgtcgagagc aataatggaa ggaggagttt ttgtcatagt gcccccattt
      901 tacatcagac actctataaa aaaagaccgt agaagttcta cggtctttgg tgcttactgt
      961 gttccaccga ggaagaggtc ggttcgtgcc cagaggatga tcgcttcttg gctttgaggc
     1021 atttgccgca acgaaatcca cttcaaccaa gcaaaggtgt ctgccccatt ccaaatggga
     1081 gcctgccgcc aggtaaagtc ctgtccgcga taggctgaga ccagtccagg gtctaattca
     1141 taggaggtga tgacaaaatc aggcgtattg ttcgggtcaa ccgcatcgac cacttgcacg
     1201 ggattttcac ggacagccca ttccaacgcc ggtgagttga cgccgatgat cgcaaccgtc
     1261 gcagaataga tactgcctct gccaatgtct gaaacctgat gaaccgtatc tcgcaccaat
     1321 tgggcttggg ttgggacgtt aggaagccac catagttcgg gggcgcttaa tccgcgcaag
     1381 ccggtagcac caaataagcc acctacactc agcagcccca agcagattgc catgccccac
     1441 acgccaccca accgtgcaat gcgaatggac caacccgccg ccactaagac caaactgata
     1501 ataagcaaga agatcgaacc aaccaacatg cctgcccgca aacgataatc gggtgtatcg
     1561 accaatatcc agctaaagcc cgcagtgcca agccagatga atacccaaat gaacgtggtt
     1621 aataaaacca cacccgctac ttcgcgacgc tcttcgggga aaatgctaag gtgacgtact
     1681 aattccaaag cagccaaggc attcaatgga atgagcgccc aagccaggtc gtacatttgg
     1741 cgacctgaca ggaagatcac cagcaacaag gcaacaaaga accaaatgct caagaaaacg
     1801 atgcggcgca gaccacccat ccaaccgcgg agtagagcca tacctgccag caacagagca
     1861 aaaggctgat acacaaacaa agagatgatc aacatccctt gtgtaacggg acccgtcgct
     1921 gcccatgttt tgaaaaaagc agggatggaa gaaaatgcag cgccaatacc accgggcaca
     1981 ataaagaaga gagagccagc aaagataaag gcaagtgcaa aagttataag gttagaagat
     2041 ttgaaaaact ctttcccaaa ggcagaagac gaacgatctc gctttcgttt ttctgtgggt
     2101 gtgttttctt cttctgatga ttcttctgtg cggcgggcga gcagtttggc aatgccataa
     2161 gtgatgccga tccctaacag acccagccaa gcggacggtc cgctgaggag ggcaagggcg
     2221 gcggtaatag cagcccaggt ccatttattt ttattgataa agccgacggt aaaggcgagg
     2281 aatgtgatgg cgagaatggg gctggtgatt tggcgagaga tggcgaccaa gccggggtcg
     2341 agggcaagga agaa
//
LOCUS       DAOQFT010000413         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_29473_length_2354_cov_6.297521, whole genome shotgun sequence.
ACCESSION   DAOQFT010000413 DAOQFT010000000
VERSION     DAOQFT010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29473_length_2354_cov_6.297521"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLR93_10130"
     CDS             complement(<1..697)
                     /locus_tag="PLR93_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNH27368.1"
                     /translation="MSIKEDLIKKLKEKNAKVAILGLGYVGLPLAVVFGEAGFKVTGV
                     DPDKRKVEALNKGESYIPDVKTEAVAKLVKNGMLTATTDFSVLKEMDAVSICVPTPLR
                     QTGDPDMSFIVSAMEELSKYMHKSMVVVLESTTYPGTTRELLLPKLGTEHNLTVGEDW
                     FLAFSPERVDPGREDFTTINTPKVMGGITHACGEVATAWYEGAIKTVHHVSTAEAAEM
                     SKLLENTFRMINIG"
     gene            complement(716..1360)
                     /locus_tag="PLR93_10135"
     CDS             complement(716..1360)
                     /locus_tag="PLR93_10135"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNH27369.1"
                     /translation="MSAAETLERIAHEVSVCTKCALHESRKKAVTGEGPVDAEIMFIG
                     EGPGFHENEQGRPFVGASGKFLDQLLEQAGLTRSDVFIANVVKCRPPANRDPQPEELM
                     ACGEYLDGQIEAINPSIIVTLGRISMGRYFPGAKITSVHGQMQNIDGRFIIPMYHPAA
                     ALHQPALRPSILADFAKLPDQLNEARTALGRKVVEKKKNVVVQQPEKPQQLSLF"
     gene            complement(1370..1828)
                     /gene="rimI"
                     /locus_tag="PLR93_10140"
     CDS             complement(1370..1828)
                     /gene="rimI"
                     /locus_tag="PLR93_10140"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNH27370.1"
                     /translation="MAEVLIRKMIHADLEQVIEIDQISFSLPWPKRSFQYELDENFTS
                     RCWVAEVDGRIAAMMVGWMIIDELHVATIATHPDYRGQGIGKKVLVHALLAARKEGVI
                     RAFLEVRQTNEAALNMYRSLGFVEDGRRKEYYKDNNEDAILMSLNDMTHL"
     gene            complement(1834..>2354)
                     /gene="tsaB"
                     /locus_tag="PLR93_10145"
     CDS             complement(1834..>2354)
                     /gene="tsaB"
                     /locus_tag="PLR93_10145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNH27371.1"
                     /translation="GMTMASVNALGVAIGPGSFTSLRVGLSLVKGIALARNLPVMGIS
                     TLDVIAAAQPISDTSLVAVLQAGRTRVAYGVYQSVDGQWQAEGPVRSGTLDEMLSQFE
                     SATLIAGELTSEERKKVFKAKHFQLASPVLCVRRPAVLAELAWARWQDDDADDAVSLA
                     PIYLHVAGTPIQ"
BASE COUNT          597 a          670 c          481 g          606 t
ORIGIN      
        1 gaccgatgtt gatcatgcgg aaggtgtttt ccaacaactt tgacatttct gcggcttctg
       61 ccgtggaaac atggtggacg gtcttaatcg caccttcata ccacgccgta gcaacttcgc
      121 cacatgcatg cgtaatacca cccatcactt tcggtgtgtt gatggtcgtg aaatcttcac
      181 gtccagggtc cacacgctct ggcgagaatg ccaggaacca gtcttcgcca acggtcaggt
      241 tatgttcagt accaagtttc ggcaggagca attcgcgagt cgtgccgggg tatgtcgtgg
      301 attccaacac aaccaccatg cttttgtgca tatacttgct caactcttcc atcgccgaaa
      361 cgatgaaaga catatctggg tcgccggttt ggcgcaaagg cgttggcaca caaatgctga
      421 cggcgtccat ttctttcagt acagaaaaat cggttgtagc agttaacata ccattcttga
      481 caagcttcgc aactgcttcg gtcttaacat ctgggatata agactcgcct ttattgagcg
      541 cctcgacttt acgtttatct gggtctacac ccgtgacttt gaatcccgcc tcaccaaata
      601 ccactgccaa cggcagcccc acatacccca agccaaggat cgcaaccttg gcattctttt
      661 ctttcaattt cttgattaaa tcttccttga tagacatttt cttttacctt caatttcaaa
      721 atagacttaa ttgttgtggc ttttcaggtt gctgaacaac cacatttttt ttcttttcaa
      781 caactttcct tcccagtgca gtacgggctt cgttaagttg atccggaagc tttgcaaaat
      841 ctgccaaaat ggatggacgc aaagctggct gatgaagcgc tgccgcgggg tgatacatgg
      901 gaatgataaa ccttccgtca atattttgca tctgcccatg tacagaagtg atctttgcgc
      961 cggggaaata tcgccccatg gaaatacgcc ccaacgtcac aatgatactc ggatttatag
     1021 cttcgatctg accatccaaa tattcaccac atgccattaa ttcttcaggt tgcgggtcac
     1081 ggttggcagg cggacggcac ttgaccacat tcgcaatgaa cacatccgaa cgagtcaaac
     1141 cggcttgttc caacaattga tctaaaaact tacccgatgc ccccacgaac ggacgtccct
     1201 gttcattctc atgaaatccc ggaccctccc caatgaacat gatctccgca tctaccggac
     1261 cttcacccgt cactgctttc ttgcgcgact catgcaaagc acacttcgta caaacagaaa
     1321 cttcatgggc aattcgttca agggtttcag cggcagacat gcagtctcct tacagatgcg
     1381 tcatatcgtt caacgacatc agaatggcat cctcattatt atctttgtaa tactcttttc
     1441 ttcgaccatc ttccacaaag cctaaacttc gatacatgtt caaagccgct tcatttgttt
     1501 ggcgcacttc aagaaaggcg cggatcaccc cttcttttct cgccgctaaa agggcatgaa
     1561 ccagtacttt ctttccaatg ccctgcccgc gatagtcggg atgcgtagca atcgttgcga
     1621 catgcaattc atcaatgatc atccagccca ccatcattgc agcaatacga ccatccactt
     1681 cagccaccca acaacgcgac gtaaagtttt catctaattc gtattgaaag gaacgcttcg
     1741 gccacgggag tgaaaaagaa atctgatcga tctcgatcac ctgttccaaa tctgcatgga
     1801 tcatcttacg aattagaact tcagccataa tctttactgg atcggtgtgc ctgctacatg
     1861 caagtaaatc ggcgcaagcg acacggcatc atcagcgtca tcatcctgcc aacgcgccca
     1921 agctaactct gccaatacag ccggtctgcg cacacacaaa actggagacg ccaactggaa
     1981 atgttttgcc ttgaagactt tctttctctc ttcggatgtt aactccccag cgatcaaagt
     2041 cgcactctcg aactgactca acatctcatc cagggttccg cttcttacgg gaccctcagc
     2101 ctgccactga ccatctacac tttgatacac cccgtatgcg actcgcgtcc gcccggcttg
     2161 caagactgcc accaatgagg tgtctgaaat gggttgtgcg gcggcgatta catccaatgt
     2221 tgagatgccc atcactggca ggttacgagc caaggcaatg cccttcacca aagacaaccc
     2281 gactctcaaa ctcgtgaacg aaccaggtcc gattgccacg cccaaagcgt tgaccgaagc
     2341 catggtcatg ccgc
//
LOCUS       DAOQFT010000414         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_30031_length_2326_cov_6.270806, whole genome shotgun sequence.
ACCESSION   DAOQFT010000414 DAOQFT010000000
VERSION     DAOQFT010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30031_length_2326_cov_6.270806"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLR93_10150"
     CDS             complement(<1..240)
                     /locus_tag="PLR93_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27372.1"
                     /translation="MDKFKTMHRRFTVGGLALVLLASLFLTASAPSDPDFQIGVPLDQ
                     AIVYSGGESSNLRDYDPATTYSAGDKLIFSGLVQLD"
     gene            complement(233..1594)
                     /locus_tag="PLR93_10155"
     CDS             complement(233..1594)
                     /locus_tag="PLR93_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNH27373.1"
                     /translation="MHKKLILFLTLLTILLMPAKVQAALHADVQADTATLDFPNTATF
                     SATLSASAEITSVTLEYGSEQLNCGEVIAKAFPDFSPSPSIDVSWTWDMRQSGSLPPG
                     TTIWWRWRYVDSAGKEYLSDTNTVIWLDNDHDWQTATGTNIRIHYYGASQSFAQSMVD
                     AGLEGLRRNKDQAGMTPDIPVDIYVYPSYDEMRDAILYEPQWTGGLAYSNFSIIIMGT
                     SGDANFDKATVIHEVTHVLVGRNAFTCIGFIPTWLNEGLAVYNEGPLDESMKAQFDIA
                     LRDDTFFPVRSLSGNFSEIPEKALLSYAQSHSVVKFMLETYGQEKMSQLLQALSDAEP
                     VDDALVQIYGVDTDGLDAAWRESLGLQPLVVAQATSQPTPTYVPTYVPIAGIPQAITA
                     TPGAIPTSSSTNNSEPSFSGPPIVLTVVMLCICLVFLTIIGVVVLGIVLRNNKGNGGK
                     KNG"
     gene            1807..2289
                     /locus_tag="PLR93_10160"
     CDS             1807..2289
                     /locus_tag="PLR93_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNH27374.1"
                     /translation="MQRNKFIIGGVLILAAVVYLIASSTQASAEYFMTVDELNSEGSA
                     AINKSVRLSGAVIGDTIQYDASTLTLTFEVAHVPGDNAEIEAQGGLAAVLHEAVMDTS
                     RARIKVQYEGVMPDLLRNEAQAIMTGKLGDDGVFHAEELLLKCPTKYEEAVPEQASAN
                     "
BASE COUNT          630 a          561 c          571 g          564 t
ORIGIN      
        1 gtcgagttgc accaaaccgc tgaagatgag tttatcgccc gcactatagg tcgtggcggg
       61 gtcgtaatca cgcaaatttg aagattcacc accactgtaa acaatcgcct gatctaacgg
      121 aacaccaatc tgaaaatcgg gatcgctggg tgcgctggca gtaaggaaga gagaagcaag
      181 taaaacaagc gccaaaccac caacggtaaa gcgccgatgc atggttttga atttatccat
      241 tcttctttcc tccattgcct ttattattgc gcagcacaat tcctaaaacc acaacaccga
      301 tgatcgtcaa aaagacgaga cagatacata gcatcacaac ggtcaacaca atgggcggac
      361 cactaaaaga aggctcggaa ttatttgttg acgatgaagt tggaattgcg cccggtgtgg
      421 cggtgatggc ttgaggtatg cccgcaatag gaacataggt cggcacatag gtcggcgtgg
      481 gctgcgatgt tgcttgtgcc accaccagcg gttgaagccc aagcgactca cgccaagcgg
      541 catcgagccc atcggtatcc acaccgtaaa tttgtactaa ggcatcatcg acaggttcgg
      601 catcgctaag cgcttgaagc agttgactca tcttctcttg cccataggtc tcaagcataa
      661 acttgaccac actatgagat tgagcgtatg aaagcaaggc tttttctggg atttcagaga
      721 aatttccgct caaagaacgg acagggaaaa aggtatcgtc acgcaaggca atatcgaatt
      781 gcgccttcat gctttcatcc agcggacctt cgttatacac ggcgagacct tcattcaacc
      841 aagtaggaat aaacccaata caagtaaatg cattgcgtcc tactagcaca tgggtcactt
      901 catggatgac ggtggcttta tcaaaattgg catcgccaga agtgcccatg ataatgatgc
      961 tgaagtttga gtatgccaaa ccgcctgtcc actgcggttc atagagaatg gcatctcgca
     1021 tttcatcgta actggggtag acgtaaatat cgacagggat atctggcgtc ataccggctt
     1081 ggtctttgtt tcggcgtaga ccttccaaac cagcgtccac catagactgg gcgaaacttt
     1141 ggcttgcacc atagtaatgg atgcgaatat ttgtgcctgt tgcagtttgc cagtcatggt
     1201 cattatccaa ccagatgact gtattcgtat cgcttagata ttccttgcca gcagagtcaa
     1261 catagcgcca gcgccaccag atggtagtgc ctggcggaag ggatccgctc tgacgcatat
     1321 cccaggtcca ggagacgtct atggatgggc tgggtgaaaa atcggggaag gctttggcga
     1381 tcacctctcc acagttcaat tgttcgctgc catattccag ggtgactgag gtaatttctg
     1441 cagaagcact aagagtagca gaaaaagtag ccgtgttggg aaagtccaag gtggctgtat
     1501 ccgcttgaac gtcggcatga agcgcagcct gaaccttggc aggcatgagc aaaattgtga
     1561 gtagtgtcaa aaacaggatg agctttttat gcatcctttt cctccattcg tttttgcaac
     1621 tataccattt ttcaaagcca ctttcctccc tctgatgtgg agttaaaaac cgtcatggag
     1681 aaataggata aattcacgca gaattcatga catccatcag accttaaact cagccctatt
     1741 taatgtcatc atgtaaaatg gacatagatt gtttgattta gtaacacaaa cctttggaga
     1801 cctcctatgc aacgaaacaa atttatcatc ggcggcgtgc tcattttggc ggctgtcgtt
     1861 tatctcatcg cctcgtccac acaggcgagc gcagaatatt tcatgacggt ggatgaactt
     1921 aactcagaag gttccgctgc catcaacaaa agtgtccgtc tttcaggcgc agtcattggc
     1981 gacaccatcc aatatgatgc atctactctg accttgacct ttgaagtggc acacgttcct
     2041 ggcgataatg ccgagatcga agcccaaggc ggtttggctg ctgtgcttca cgaagcggtg
     2101 atggacacca gccgggcacg catcaaggtg cagtatgaag gcgtgatgcc cgacctgctc
     2161 cgtaacgaag cgcaggcgat catgaccgga aagctgggtg atgacggcgt tttccatgct
     2221 gaggagcttt tgctcaaatg ccccacaaag tacgaagagg cagtgcctga acaggcatca
     2281 gcgaactaac caaatgacct gatgggtgtt ctatccatca ggtttt
//
LOCUS       DAOQFT010000415         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_30086_length_2323_cov_4.691358, whole genome shotgun sequence.
ACCESSION   DAOQFT010000415 DAOQFT010000000
VERSION     DAOQFT010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30086_length_2323_cov_4.691358"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLR93_10165"
     CDS             <1..229
                     /locus_tag="PLR93_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27375.1"
                     /translation="EDGIPDGQEYLDGTDPTNKNHGDPLKLFAVNIDRYKSHLILAGL
                     AVVFLDWGFAHLIMGFYRKSQAKKLKRGEST"
     gene            complement(264..536)
                     /locus_tag="PLR93_10170"
     CDS             complement(264..536)
                     /locus_tag="PLR93_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27376.1"
                     /translation="MSRPIEEITVLDIVNSVDPIKRIDVCPLGLETHGMNLCPLHKRL
                     SEATAAVELAFASSTLAELVQESKTPLCASIPLADSKSGGRPGARG"
     gene            complement(521..739)
                     /locus_tag="PLR93_10175"
     CDS             complement(521..739)
                     /locus_tag="PLR93_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNH27377.1"
                     /translation="MKATVMYGAGDVRVENVPDARIKESTDALVVVTRAAICGSDLWP
                     YKGMPTSEVGRRMGHEFIGVVASFCHAR"
     gene            complement(755..1720)
                     /locus_tag="PLR93_10180"
     CDS             complement(755..1720)
                     /locus_tag="PLR93_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH27378.1"
                     /translation="MQIRKLGNSGLKVSAMGLGCMRMSFGDKPTDKKEMIDFLHKSVE
                     RGVTFFDTAEVYGPFTNEELVGEALESFKGQVVIATKFGFKHGENGPHPTAGLDSRPE
                     QIKRVAEESLKRLRVEVIDLFYQHRPDPSVPMEDVAGAVKDLIQAGKVKHFGLSESNA
                     DQIRAAHAVQPVSALQSEYSIWWRAIEENGVLATCEELGIGLVPYSPLGRGYLTGKVD
                     ENTTFDKSDIRSRNPRFTQDAIKANRGVVELLERIAKQKNATPAQIALAWLLAQKPWI
                     VPIPGSRKLERLDENNGAVNLQLSANDLGEIRSAMEKITVVGDRY"
     gene            complement(1724..2107)
                     /locus_tag="PLR93_10185"
     CDS             complement(1724..2107)
                     /locus_tag="PLR93_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNH27379.1"
                     /translation="MKIQEVSEKYSLSVDTLRYYERVGLIPPVTRTLSGIRNYSEQDE
                     RRVEFIKCMRSAGLPIEVLIDYMELVQQGDSTIEARKDILKEQREQLIARMEEMKKTL
                     DILDYKIRVYEGNILETEKKIKVME"
BASE COUNT          567 a          599 c          528 g          629 t
ORIGIN      
        1 tgaagacgga attcccgacg gacaagaata tttagacggg actgacccaa ccaacaaaaa
       61 tcatggtgat ccgttaaagc tattcgcagt gaacatcgac aggtataagt ctcacctgat
      121 tcttgcggga ctggctgtcg tttttcttga ttggggattc gcccacctga tcatgggatt
      181 ttatcgtaag tcgcaagcga aaaagctaaa gcgcggcgaa tcgacataag gggctgttct
      241 tgaaggacaa caactcgttg agtttacccg cgtgcgcctg gacgtccacc agatttgcta
      301 tcagcaagtg gaatgcttgc gcaaagtggt gtctttgatt cctgcacaag ctctgccagc
      361 gtcgaactgg caaaggcaag ttcaactgca gccgttgctt cagaaagacg tttatgcagc
      421 ggacacaaat tcataccgtg cgtttccagt cccagagggc aaacatcgat gcgtttgatt
      481 gggtcaactg aattaacaat atccagaact gttatctctt ctatcgggcg tgacaaaacg
      541 aagccaccac gccgatgaat tcatgcccca tacggcgacc gacttcgctc gttggcatcc
      601 ctttataggg ccataggtca ctgccacaaa tggcagcgcg agtgacaacc acaagagcat
      661 cggttgattc tttgattcga gcatcaggga cgttctcgac acgaacatca cctgctccat
      721 acataactgt tgctttcatt tacttttctc ctaattaata tcgatcaccg accacggtaa
      781 ttttttccat ggcactgcgg atttcaccga gatcatttgc agagagttga aggttcactg
      841 cgccgttgtt ttcatcaagc cgttccagtt tgcgcgaacc tgggatggga acgatccacg
      901 gtttttgcgc gagcagccaa gccaatgcga tttgagcggg tgtggcgttc ttttgtttgg
      961 caatgcgttc caacagctcc actacaccgc gattggcttt gatagcgtcc tgagtgaagc
     1021 gtgggttgcg gctgcgaatg tcagatttat cgaaagtggt gttctcgtcc actttgcccg
     1081 tcaaatatcc gcgcccaagc gggctgtagg ggacgagtcc gatgccgagt tcttcgcaag
     1141 ttgcgaggac tccattttct tcgatggcac gccaccagat ggagtattcg ctttgaagcg
     1201 ctgaaacagg ctgaacggcg tgtgcggcgc gaatttgatc ggcattcgat tcagataaac
     1261 caaaatgctt tactttgcct gcttgaatca aatccttgac cgcgcctgcc acatcttcca
     1321 tagggacact gggatcgggg cgatgctgat agaacagatc aatgacttcc acgcgtagac
     1381 gtttgagcga ctcctccgcc acgcgcttga tctgctcggg acggctgtcc aagccagcgg
     1441 tcggatgcgg accattctct ccatgtttga atccaaactt tgtcgcgatc accacctgac
     1501 ctttgaacga ctccaacgct tcgcccacca gttcctcatt cgtgaatgga ccatacactt
     1561 cagccgtatc gaagaaggtc acgccgcgct caactgattt gtgtagaaaa tcaatcatct
     1621 cttttttatc ggtgggctta tcgccaaagc tcatgcgcat acaacccaag cccatggctg
     1681 aaactttcaa cccgctgttg cctagtttcc gtatttgcat aatttactcc atcaccttaa
     1741 tctttttttc tgtttccaaa atattgcctt catacactcg aattttgtaa tccaaaatat
     1801 cgagtgtctt tttcatctct tccatacgtg caatcaattg ttcgcgctgt tctttcaaaa
     1861 tatctttacg cgcctcaatg gttgagtcac cttgttgaac aagttccata taatcgatca
     1921 acacttcgat aggcaagccc gcgctgcgca tgcacttgat gaattccaca cgacgctcat
     1981 cctgctcgct gtaattacga ataccactca aggtgcgtgt caccggcggg atcaacccca
     2041 cccgttcgta atacctcagg gtatcgaccg aaagactgta tttttcgctg acttcttgta
     2101 tcttcatagg tcacctcgga aggcagtgta acacctggag tcaactccaa gtcaagggct
     2161 gaatttgacc aaaatcggac ttaaacaaaa acagagcgca tccttgcgct ctgtttttgt
     2221 ttagagacga aatttatccg agcagaattg ccagtaaatt tcctgttgct tcaaagattc
     2281 ttttccaagt agaacgagcc tttttctttg aaatggtgtg tgg
//
LOCUS       DAOQFT010000416         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_30374_length_2311_cov_7.351064, whole genome shotgun sequence.
ACCESSION   DAOQFT010000416 DAOQFT010000000
VERSION     DAOQFT010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30374_length_2311_cov_7.351064"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..2261)
                     /locus_tag="PLR93_10190"
     CDS             complement(348..2261)
                     /locus_tag="PLR93_10190"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HNH27380.1"
                     /translation="MKTIRRLYFYAVAAISIEVVLWGIIGLLRSILGAQDISNNATTL
                     ASALALIFVGVPIFLIHWGWAQNVSSKDSEEHSASIRAVFLYGILLGTLIPVVQNLLA
                     LINRTFLSTVHLSSYRAVVGGYQTWLDNLIAIIINLLIAAYFWNVLRSNWKTITEPES
                     FAEVRRLYRFVWVLYGLLMAVYGVQQAIAYAFSFPTGLLGEVGRETAVNAIALMVIGT
                     PIWYYTWRLLQDALTDTDEKESYLRLGLLYLLTLGGILVFLFSCGDLLYLIFLQVLGD
                     GKSASEFVRELGGALSVTVPFGVVWAYYSKWLNQQFAFDENLPRRAGKHRLYIYILSF
                     VGLTATTGGLISLFGVLIDLTLGNRYLSDSGMREPLAGAFASLLVGLPLWLLNWRVAQ
                     SQALADSDLGDHARRSVIRKAYLYLELFATVIGGMVSAGFLVYNLINAVLGNGISDFW
                     TAVLNQLAVLIVFIVLLVYHLLALRADGTARADVLLEKQSKYQMVVFDADGKFGNAVK
                     SVFAKRAPQVPLTVVDANSELPADLNADAVILPGSLAVNTPEKVEAWLRSFGGNKLIV
                     SDEAAGVFWMNDFEQAADSAKSLAEGQDIRPQSSKRTASIWTVVAYVFAALFACQLLF
                     GLLMFGVSMVTGF"
BASE COUNT          712 a          623 c          516 g          460 t
ORIGIN      
        1 gcgggaatcc aacacgtaat gcccaactca aggttggtgg ctgaaagtcg caaacgacct
       61 tcgtcggtcg cgatcaaaat attcgaaaga acagggagtg tactgcgcga ggaaaccgcg
      121 cgcgaaacaa tgctaagacc acgggcaaga ttctcttgaa ggacagttac tttcatggtg
      181 cgcctcgctt aataattagt cagaattttc aaaagtatat catccaatcc caaagggatg
      241 gaaggattat agataacaat cgcgaattaa aacaaatccc gaaggaatag cacagcgttt
      301 acaagccatg tcactccttc gggcagaaat tttttgctac ttctttacta gaagcctgtc
      361 accatcgaga ccccaaacat cagcagccca aacaacaact gacacgcaaa caaggctgcg
      421 aaaacatagg ctacaactgt ccaaatagag gcagtgcgtt tggaagactg cgggcggata
      481 tcctgccctt cagccagaga cttcgccgaa tccgctgcct gctcgaaatc attcatccaa
      541 aatacgcccg ccgcctcatc actgacgatg agcttgttgc cgccaaagga ccttaaccac
      601 gcctcaacct tttctggcgt attcacagcc aaagagccgg gcaaaatcac agcatctgca
      661 ttcaagtcag cgggcaactc tgagttcgca tccaccaccg tcaatggcac ttgaggcgca
      721 cgtttggcaa atactgactt aactgcattt ccaaacttgc catctgcatc aaaaaccacc
      781 atctgatact tcgactgttt ttccagcaat acatcagctc gtgcagtgcc atcggctcgc
      841 aaagcaagca agtgatagac cagcaaaaca atgaacacaa tcaacaccgc gagttggttg
      901 agcaccgccg tccaaaaatc ggagatgcca tttcccaaaa cagcgttgat caggttgtag
      961 accaaaaaac cagccgagac catcccacca atgaccgtag caaacaattc gagatagaga
     1021 taggctttgc gaatcactga acggcgagca tggtcaccca ggtcactatc tgccaacgct
     1081 tgagattgcg ccacacgcca gttcagcagc cataaaggta agcccactaa caacgaagcg
     1141 aacgcacccg ccaaaggctc tctcattcca gagtcgctta ggtagcgatt gcccaaggtc
     1201 aaatcgatta aaacgccaaa cagagagatc aagccgccgg tcgttgctgt aagccccaca
     1261 aacgaaagga tgtagatgta caggcgatgt ttccccgccc ggcgtggaag attctcatcg
     1321 aaagcaaatt gttggttgag ccatttgcta tagtaagccc acaccacacc gaacggaacg
     1381 gtgacagaga gcgccccacc caattcacga acaaattcac tggcagactt gccatccccg
     1441 agcacttgta aaaagatcag gtacaacaag tcgccgcatg aaaatagaaa gacgagaatc
     1501 ccaccgaggg taagtaagta gagcagccca aggcgcagat atgattcttt ttcgtcagta
     1561 tccgttagcg cgtcctgtaa gagccgccag gtgtaatacc aaatcggcgt accgatcacc
     1621 atcaaagcaa tcgcgttgac tgcggtttca cgtcccactt ctcccagcaa accggttggg
     1681 aacgagaagg catatgcaat ggcttgttgc acgccgtaga ctgccatcaa caacccatac
     1741 agcacccaca caaaacgata caaacggcgt acttccgcga agctttcggg ttcggttatg
     1801 gtcttccaat tggaacgtag cacattccag aaataggcgg cgatcaacaa gttgataatg
     1861 atcgcgatga gattatccaa ccaagtttga tacccgccaa ccacagcccg gtacgaagag
     1921 agatgcaccg tggaaagaaa agttcggttg ataagcgcca acaagttttg cacaaccgga
     1981 atcagcgtgc ccagcaaaat tccatacaga aaaaccgcgc ggatgctggc tgaatgttcc
     2041 tcggagtctt ttgaagaaac attctgcgcc caaccccagt gaatcaggaa gatgggcacg
     2101 cccacaaaga tcagcgccaa tgccgaagca agtgtggtgg cattgttact gatatcttga
     2161 gcgccaagga tggaacgcag caacccaata atgccccata acaccacttc aatgctgatg
     2221 gcagcaaccg cataaaaata aagacgacga attgttttca tagttgatcc tcagtcatta
     2281 cgagttttgc aatgacatga aaaattacgg g
//
LOCUS       DAOQFT010000417         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_30516_length_2304_cov_5.626501, whole genome shotgun sequence.
ACCESSION   DAOQFT010000417 DAOQFT010000000
VERSION     DAOQFT010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30516_length_2304_cov_5.626501"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLR93_10195"
     CDS             complement(<1..287)
                     /locus_tag="PLR93_10195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH27381.1"
                     /translation="MKQHIYWHTTAQMPEGNNLTPIPTKADVAIIGGGYTGLSAARTL
                     AKQGVSVVVLEAETIGWGASSRNGGMTLTGLKPAMQTVIKNYGRELAKELF"
     gene            417..1163
                     /locus_tag="PLR93_10200"
     CDS             417..1163
                     /locus_tag="PLR93_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27382.1"
                     /translation="MEWTGFLGFFGFCIAGLAGPLSIIVLYFVIRNIWKAHAYRRDLT
                     LHGVCAPAVVLSAWNNYSSGGGKHSAANTAHVTFEVEVMPPGQAPFRAKFKEQLSIRN
                     GEWFVIGPRKEEVGKKIWVAYDPKDPKRMVIDHFDSDHEFMLKRRAFEKQDHELQNTR
                     KTGEDANAEILEIEDMKLSNIIENGFQSVMRLKLRVEPRSGTPYETETVGMFSNTGLH
                     KYTVGKKMTVKIDRADKYKVALVGPIQQEA"
     gene            complement(1220..>2304)
                     /locus_tag="PLR93_10205"
     CDS             complement(1220..>2304)
                     /locus_tag="PLR93_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH27383.1"
                     /translation="LAAYLLGRIPPQEERLLPRITAWTQQIRDPEVRVALLTTSLTRM
                     RKETPNQFLALVREYLHPERSRSWSNGIQALIPMITDSDFENLPAIFDLVEPIVEEAP
                     STLQYDLTDLIVTLYRASASETIFMLKHILSTTENQMTAVTMRRISPDFPPPLQKELR
                     DLLRPQPLTLIKPAEPDDFIDESAPPEEKVKPARKPRKKKMDNSRIIYLHGSDSNSQS
                     GKARQFAEKFPGMVTPDFTGDFDERMAQLKKIIGRKKGWTIIGSSLGGLMGTVFTCQK
                     PTHVRKLILLAPALLKDPFGSYLNLESVSVPTTVIHGTADDVVPLDEAREYAEKFFTD
                     LKYIVVDDGHRLQEAFKELDWEEILN"
BASE COUNT          553 a          470 c          646 g          635 t
ORIGIN      
        1 tgaaataatt cctttgccaa ttcccgccca taatttttga tgaccgtctg catggcaggc
       61 tttagtcctg tgagggtcat gccgccgttg cgggagctgg caccccagcc gatcgtctct
      121 gcttcaagta cgaccacact cacaccttgt tttgccaagg ttcgtgccgc gctcaaaccg
      181 gtgtatccgc caccgatgat cgctacatcc gctttggtgg ggatgggggt aaggttattg
      241 ccttcgggca tttgggcggt tgtatgccag tagatgtgtt gtttcatgag tcggctccgt
      301 tgtgtcttaa ggaataggac aattgtgcca tattttaagt caccttaatc agatattatc
      361 gctcctaata ttattagtcg ttctataaat tttcattcaa ataaatagag gttaacatgg
      421 agtggacggg ttttcttggc ttttttggtt tttgcattgc tggattggca ggtcctttat
      481 caattattgt gctttacttt gtaattagga atatttggaa agcacatgca tatcgtcgcg
      541 atttgacctt gcatggagtc tgtgccccag cagtagttct ttcagcatgg aacaattatt
      601 catctggcgg cggaaaacat agtgcggcta atacagccca tgttactttt gaagttgaag
      661 tgatgcctcc tgggcaagct ccgtttagag ctaaatttaa ggagcagctc tctattcgaa
      721 atggtgaatg gttcgttata ggacctcgca aagaagaagt gggcaagaaa atctgggtgg
      781 catatgaccc taaagatcca aagcggatgg tgattgacca ttttgactct gaccatgagt
      841 ttatgttaaa gcgtcgggca tttgaaaaac aagaccatga attgcagaat actcgcaaga
      901 ctggtgaaga tgcaaatgct gagatccttg agattgaaga tatgaagtta tccaatatta
      961 ttgagaatgg tttccaaagt gtaatgcgct taaagttgcg cgttgaaccc agaagcggga
     1021 cgccttacga aacagaaaca gtagggatgt ttagtaatac ggggttacat aaatacactg
     1081 tcggtaaaaa gatgactgtg aaaattgacc gagcagataa atacaaggtt gctctggtgg
     1141 gtccaataca gcaggaagca taagggctat attgcgccta ttaaatcaaa caccgccaaa
     1201 ataaatggcg gtgtttgatt taattcaata tctcttccca atctaattct ttaaacgcct
     1261 cttgcaagcg gtgaccatcg tccacgacga tgtatttcaa gtcggtgaag aatttctcgg
     1321 cgtattcgcg cgcctcatcg agcggaacga cgtcatccgc tgtcccgtgg ataacggttg
     1381 tgggaacaga aacagactcg agattaaggt aagagccgaa ggggtctttg agcaaagccg
     1441 gagcaagcag aatcaacttg cggacgtgag tcggtttttg gcaggtgaag accgttccca
     1501 ttaatccgcc cagtgatgag ccgatgatcg tccagccttt tttgcgaccg atgatctttt
     1561 tgagctgtgc catgcgttca tcgaaatcgc cggtgaaatc gggggtgacc atgccgggga
     1621 atttctctgc gaactgacgg gctttgccgc tttgactgtt gctgtctgag ccgtgtaggt
     1681 agatgatgcg ggaattgtcc attttctttt ttctcggctt gcgcgcaggt ttgacttttt
     1741 cttcgggtgg tgcgctctcg tcgatgaagt catcgggttc ggcaggtttg atgagagtaa
     1801 gtggctgcgg gcggagcagg tcgcgcagtt ctttttgcaa tggcggcggg aaatcgggcg
     1861 agatgcgccg catagtgaca gcggtcattt gattctcggt ggtgctgagg atatgcttaa
     1921 gcatgaagat ggtttcactg gcagaggcac gatagagcgt gacgatgagg tcagtgagat
     1981 cgtattgcag ggtggatggt gcttcttcaa cgatcggttc gacgaggtcg aagatggcag
     2041 gcaggttttc aaagtcgctg tctgtgatca tggggatgag cgcttgaatg ccatttgacc
     2101 aggaacggga gcgttcgggg tgtaagtact cacgcacaag ggcaaggaat tgattcggtg
     2161 tttccttgcg catgcgggtg agactggtgg tgagcaatgc cacacgcact tccgggtcgc
     2221 ggatctgttg cgtccatgca gtgatgcgtg ggagcaggcg ttcttcctgc ggtgggatac
     2281 gcccaagcaa ataagcggcg agca
//
LOCUS       DAOQFT010000418         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_30610_length_2300_cov_7.206236, whole genome shotgun sequence.
ACCESSION   DAOQFT010000418 DAOQFT010000000
VERSION     DAOQFT010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30610_length_2300_cov_7.206236"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLR93_10210"
     CDS             complement(<1..379)
                     /locus_tag="PLR93_10210"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNH27384.1"
                     /translation="MKHLWSPWRMNYIEGNKKEEGCVFCNAQSKPDSAENLIAFRGKS
                     AYVILNRFPYTSGHLMVIPFSHVATIEELDSETRAEMMELTSRCTTELRQIYKPHGFN
                     VGINMGEAAGAGVLGHVHIHIVPR"
     regulatory      465..632
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            974..>2300
                     /gene="mgtE"
                     /locus_tag="PLR93_10215"
     CDS             974..>2300
                     /gene="mgtE"
                     /locus_tag="PLR93_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNH27385.1"
                     /translation="MNPTLAMINLEALIEARDFNTLREQTKNWSAGDLAELMEPLSAE
                     KEAVIFRLLPREQAAGVFSYLSEERQEELLKAMAREDVANILNAMSPDDRTSLLEEMP
                     ANVIQHLLGLLSAEERTVASQLLGYEEDSVGRLMTPDFVRVRPQWTIAQALEHIRRYG
                     KDSETMSMIYVIDDNNKLVDDLRIRQILLASPTDTVADLMDSRFVYLKTTDNRETAVE
                     AFKDSDLNALPVTDKEDTLIGIVTVDDILDVAEEEATEDIQKMGGSEALDEPYMKIAI
                     SKMVQKRATWLIILFISEMLTATAMGNFEHEIAKAVVLSIFVPLVISSGGNSGSQAST
                     LIIRAMALGEVRLVDWWRVMRREFLSGLSLGAILGVIGFIRISVWHYLFDAYGPHWFL
                     LAVTVGIALVGIVLWGSLSGSMLPFLLKRVGLDPATSSAPFVATLVDVSG"
BASE COUNT          547 a          533 c          617 g          603 t
ORIGIN      
        1 aacgcggcac gatgtgaatg tggacatgcc ccaaaacgcc ggcgccggcg gcttctccca
       61 tattgatgcc gacattgaag ccatgcggct tatagatctg gcgcagttcc gtggtgcaac
      121 gtgaggtcag ttccatcatt tcggcgcggg tctcgctatc aagttcttca atggtcgcca
      181 catggctgaa agggatgacc attaaatgtc cgcttgtgta ggggaagcgg ttaaggatca
      241 cataagcgga ttttccacga aacgcgatca agttctcggc gctatccggt ttggattgtg
      301 cgttgcagaa tacacagcct tcttcttttt tgttgccttc aatgtaattc atacgccacg
      361 gagaccatag atgtttcatg aaaagattct agcacaaacg agattgccat ataaaaattc
      421 ttgacaagca gactgttacg ggtgtataac cgcggtgcaa attgaatggt cctcgttagg
      481 ccaggcgtct gcaacataca taagccattg tcctgaaacg tcgaaagacg ccaaagggta
      541 taccaggtga ggtcggcata aggcttcact caatgtggct gggagcaatc cttacgatgt
      601 gcagtgctaa agctcaacca gagaggcaag gttttgtttt gtgtatgtct gcctcctggt
      661 tttgtaacgg gaggctttct ttttgccagc atgtgttgcc aaggcaaatc caaccacgtg
      721 atgcaaggag gttatccgta tgagtacgga tagtagtaaa cagatgcaat ttcaggcgaa
      781 aagattttcg gcttttcggt ctgtatcttc ctacctccat gctgttcact gcccatgagt
      841 tgaccgggta aagcataaaa cgggggaggg aagctgcagg cttgaacgag tgtgaatcgc
      901 cttttcgtcg aaacggaggc gattttttgt tgcctccaac aggttctacc cattttattt
      961 ttaggagacg acaatgaacc caacactcgc aatgatcaac cttgaagcat tgatcgaagc
     1021 acgtgatttc aacacattac gcgagcagac caagaactgg tcggctggcg atctggctga
     1081 attgatggag ccgctttcgg cggagaaaga agcggtcatc ttccgcttgc tgccacgtga
     1141 acaagcggca ggcgtattca gttatttatc cgaagagcgt caggaagagt tattgaaagc
     1201 catggcgcgt gaagatgtgg cgaacatcct caatgccatg tcgcctgatg accgtacttc
     1261 gctcttggaa gagatgcctg ccaatgtgat ccaacattta ctgggcttgc tttcggcaga
     1321 ggaacgcacg gtcgcttcgc aattgctggg ctatgaagaa gattcggtcg gtcgtttgat
     1381 gacgcccgac tttgtacgcg tccgcccgca gtggacgatc gctcaggcac tggagcacat
     1441 tcgccgctat ggtaaagaca gcgaaacgat gagcatgatc tatgtgattg atgataataa
     1501 caaactggtc gatgacctgc ggattcgcca aatccttttg gcatcgccga ccgacaccgt
     1561 agcggacttg atggattcgc gctttgtcta tttgaaaaca acagacaatc gcgaaacagc
     1621 cgtagaagcc tttaaagact cagacttgaa cgccctgcct gtaaccgata aagaagatac
     1681 gttgatcggt attgttacgg tcgatgacat tctcgatgtg gcagaagaag aagccacgga
     1741 agatattcaa aagatgggtg gttctgaagc cttagacgaa ccctacatga agatcgccat
     1801 ctccaagatg gtgcagaagc gcgcaacttg gctgatcatt ttattcatca gcgaaatgtt
     1861 gactgctact gccatgggaa attttgagca tgaaattgcc aaagctgtgg tgctctcgat
     1921 ctttgtgcca ttggttatct ccagcggcgg gaactctggc tcacaggctt cgacccttat
     1981 catccgcgcc atggcgttgg gcgaagtgcg gcttgtggac tggtggcgtg tgatgcgacg
     2041 tgagttttta tccggcttgt cgctgggtgc cattctcggc gtgatcggct ttatccgcat
     2101 ttctgtttgg cattaccttt tcgatgctta cggtccacat tggttcttat tagcagtaac
     2161 ggttggaatt gccctggtag gcattgtgtt gtggggttcg ctttccggtt cgatgttgcc
     2221 attccttttg aagcgcgtcg ggctggaccc agcaacatcg tccgcgccgt ttgtggcaac
     2281 cctcgtggat gtatctggac
//
LOCUS       DAOQFT010000419         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_30992_length_2283_cov_7.753142, whole genome shotgun sequence.
ACCESSION   DAOQFT010000419 DAOQFT010000000
VERSION     DAOQFT010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30992_length_2283_cov_7.753142"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PLR93_10220"
     CDS             complement(<1..1112)
                     /locus_tag="PLR93_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27386.1"
                     /translation="MKKFLSRLFNAPWYSIVFGAYPILALLSENKGQVEPSAIFRPLL
                     LSLSFSTIILLIAWLVLRKIHKAAFFSVLFLLLFFTYGHVYMYFEEKFPENTAVQWLW
                     VVWVILFVLSIVWVTRPKLTFETAASSLNGMILVLLIMSITQLDFNTSSGNSHRVGAR
                     NAPIQEDLVLPENPPDVYFFLLDSYGRSDLLQSAYGYDNSDFENALKERGFYIANESQ
                     SNYVRTEISLGSSLNMQYLQELDPAFNPQSIGRKVLWDALKHNAVRYNFDTLGYETIN
                     YASGFAWLEVTDSDQFISPPPISSGMTEFEGLFLRTTWARYAEKWGWIDSDAIMAEGY
                     RDRFNTIFNSMDSISKMPEPKFVYAHVISPHPPFVF"
     gene            complement(1130..>2283)
                     /locus_tag="PLR93_10225"
     CDS             complement(1130..>2283)
                     /locus_tag="PLR93_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27387.1"
                     /translation="RDAFMGLGLAAIAVALLLFFLRQSNEKNGDDESGQSPSEMREQL
                     WLAFITIVACLVPVILVNRHVVLPDYSRYALAPSLGVGILFSVIFERVNTASLRSGLV
                     AFLLGVSVLTHHGNAVRVADETAAVQNFWWQVAWRAPKIQEGVTLIASFPGSPLAEDY
                     FIWGPANFIYYPEKQTNNPLLIKLPAAVLDGNAVTQITTNGGVETPLRRGNYLERDFG
                     NVLVMVQSSNDSCVRFIDGRSPELNSYDDGRVAMIASYSKLDSVVTEGDVSAVPEEIF
                     GAEPERGWCYTYQQADLARQRGDWDAIPVLLKDALDKGYYPNDGLEWMPFMQAYAVQG
                     NVEEIKSMLKLVVADKYLRLQVCNSLTNLMQRETLNGDVSALIEKNICK"
BASE COUNT          695 a          564 c          475 g          549 t
ORIGIN      
        1 tcaaacacaa aaggcggatg cggtgaaatc acatgagcat agacaaattt cggctcaggc
       61 atttttgaaa tagagtccat gctattgaag atggtattga agcggtcgcg atagccttcc
      121 gccatgatgg catctgagtc gatccagccc catttttcgg catagcgcgc ccaagttgtg
      181 cgcaagaaca atccttcaaa ttcggtcatg cctgaagaaa tgggcggcgg acttataaac
      241 tggtctgaat ctgtaacctc tagccaggca aagccagaag cgtagttaat cgtttcatac
      301 ccaagggtgt caaagttata acgcactgca ttgtgcttta gagcatccca taatactttt
      361 ctgccaattg attgcgggtt gaatgctggg tctaactctt gaaggtattg catattgagc
      421 gaagaaccca atgaaatctc tgtgcggacg tagtttgatt ggctttcgtt ggcgatataa
      481 aacccacgtt ctttcagagc attttcaaag tcgctattat catatccata tgcagactgg
      541 agtaagtctg accgtccata tgaatctagc agaaaaaaat atacatcagg tgggttttct
      601 ggcaaaacaa ggtcttcttg gatgggcgca tttcttgcgc ctacccgatg cgagtttcct
      661 gaagatgtat tgaaatctaa ttgagtaatg gacataatca acaaaaccaa aatcatgcca
      721 ttcaaagaag aggcggctgt ttcaaaagtc aacttggggc gagtaaccca gacaatagaa
      781 agaacaaaaa gaataaccca caccacccac agccattgaa ccgctgtatt ctcagggaat
      841 ttctcttcaa agtacatata tacatgccca tatgtaaaga aaagcagaag aaacaaaaca
      901 gaaaagaacg cagctttgtg tattttacga agcacaagcc atgcaatcaa aaggataata
      961 gtactgaatg acaaggaaag caacaaaggt ctaaaaatag cggatggctc cacctgccct
     1021 ttgttttcag agagcaacgc caaaatggga tatgcgccaa aaacaataga ataccaaggc
     1081 gcgttaaaca accgtgaaag gaactttttc atcgttacac ctctaaaatc tacttacaga
     1141 tatttttttc gatcagtgca gatacatccc cattaagagt ttctctctgc attaggtttg
     1201 tgagcgaatt acaaacttgc aaccgcaagt atttatctgc taccaccagc ttcagcatgg
     1261 atttgatctc ttccacgttg ccttggacgg cataggcttg cataaatggc atccattcca
     1321 aaccatcgtt ggggtagtag cctttatcta aagcatcttt caataaaact ggaatcgcat
     1381 cccagtcacc tcgttggcgg gcaagatcag cttgttgata ggtgtagcac cagccacgct
     1441 cgggttctgc gccaaagatt tcctctggca cagcagacac atcaccttcg gttactacag
     1501 agtccaattt tgaataagac gcgatcatcg ccacgcgacc atcatcgtat gaattcagtt
     1561 ccggcgatct accatcaatg aagcgtacac aactgtcatt gcttgattga accatcacca
     1621 acacgttccc aaaatctcgc tccaaataat tgccgcgccg taacggagtt tccactccgc
     1681 cattggtggt gatctgcgtc accgcattgc catccaatac tgcggcaggg agttttatca
     1741 acaacgggtt gttggtttgc ttttcagggt aataaataaa attggctggt ccccaaataa
     1801 aataatcttc tgccaacggg ctgccaggga aagatgcgat caacgtcacg ccttcttgga
     1861 tcttcggcgc gcgccatgcc acctgccacc aaaaattctg caccgcagct gtttcatctg
     1921 ccacgcgcac agcattgcca tgatgcgtca acaccgaaac gcccaacaaa aatgctacca
     1981 agccagaacg caaagaagcc gtgttaacac gctcaaaaat gaccgagaac aagataccca
     2041 cgcctaaaga aggcgctaac gcatagcgtg aataatctgg caaaacgaca tgccggttaa
     2101 caaggataac cggaaccaga cacgccacaa tggttatgaa cgccagccac aactgctccc
     2161 gcatctcgct tggactttga cctgattcat cgtccccgtt cttttcatta ctctgacgca
     2221 aaaagaatag cagcaaagca actgctatgg ctgccaaccc aagccccata aaggcatcac
     2281 gca
//
LOCUS       DAOQFT010000420         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_31280_length_2270_cov_5.694357, whole genome shotgun sequence.
ACCESSION   DAOQFT010000420 DAOQFT010000000
VERSION     DAOQFT010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31280_length_2270_cov_5.694357"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..2219)
                     /locus_tag="PLR93_10230"
     CDS             complement(228..2219)
                     /locus_tag="PLR93_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH27388.1"
                     /translation="MSTSSVTIKTLLNQYRIEEFITLTPFGDLYRAIDERNGKSVALT
                     ILSKAIASDPETLKELEANNVRLQTLHHPNLNKHLGLQKTPEQAFLLEDWVDGPSLKD
                     LRGKGRLGANEALFITKYVCAGLEVLHKQNLLHLHLAPELIRINKRGEVVLCGLAGAK
                     PVNTKSEIKFSKYQAFYKSPEQLREEKLTTAADIYSLAVILYELTTSFWINGKAAPKT
                     SDAIRKTHLDVTPPAPASINHNLPDNFSRMILWALRKNPEDRLKTTTELASSLALGAQ
                     TSLDKVPLHTTPEFAPVISAALNTWTFLPPPKPTSVSADAIPLEERLAAISGPKPKPT
                     RRLGVVPVLLFLVLAGFISLFFFIRPAAETVLPTPIVYTSIAVNYTPPPTSTITPRPT
                     LTNGGRIVFTCTRGEYNQLCMINSDGSDLAQLTDMEASNYYPAFTPDSSSVLFASNRA
                     GPNFDFFVLNFTEREVFQLTVNVGNVISPDFSPDGRAIVFANRVGDGPTAIWMVNADG
                     LNPRPVYTGTGDIVSVSWSPNGEKIAYAMNTGTPQEYDIFTMDTNGRNHLKISQGLQG
                     IGGSVDWSPDGTSLLVYAGPFGDKDIFKIDAATGAFAQLTDGGNNAGASYSPDGQYIV
                     FNSLRNGDQADLYIMEADGSGLRQLTNDPEPDWGANWIE"
BASE COUNT          558 a          493 c          610 g          609 t
ORIGIN      
        1 tgaatgatac atcaaataac ctgcatgtaa aggtttttaa ccgttcagat tatgccgatt
       61 ctgttaatga atcgttattg caacttcctc tgcaatacag aatcgataca tcacgaactt
      121 actggatgca aaaatgttat agcggattca aaacttgatt cacgcttcat tccccgctct
      181 cgctggattc taaccattcc actcttcttt ccccaaaaat ttttttctta ctcgatccag
      241 ttcgcacccc aatccggttc ggggtcattc gttagctgac gtaaacccga accgtctgct
      301 tccataatat aaagatcagc ttggtcgcca ttgcgtagag aattaaagac aatgtattgc
      361 ccatctggtg agtaggatgc gcccgcatta ttgccaccat cggtcaattg cgcgaacgct
      421 cccgtcgcag catcgatctt gaaaatatct ttatctccaa acggacctgc gtacacgagt
      481 agagacgttc catcaggtga ccaatccaca ctgccgccaa tgccttgcaa cccttgcgag
      541 atttttagat gatttctacc gttggtgtcc atcgtaaaaa tatcatattc ttgaggggtg
      601 ccggtgttca tggcataggc gattttttca ccattgggcg accatgatac cgagacgata
      661 tctcccgtcc ctgtatacac cgggcgtgga ttcagcccat cggcgttgac catccaaatc
      721 gcagttggac cgtctccgac gcgattggca aaaacaatcg cacgcccatc aggagagaaa
      781 tcgggcgaaa tcacattgcc aacatttacg gtgagctgaa atacttcgcg ttcggtgaaa
      841 ttcaatacaa agaaatcaaa attcggtccg gcgcggttgg aggcaaacaa caccgaactg
      901 ctatctggtg tgaaagctgg ataatagttg ctggcttcca tatcggtgag ttgggcgagg
      961 tcgctgccat cgctgttgat catgcagagt tggttgtatt cgccgcgtgt gcaggtgaaa
     1021 acgatgcgcc caccattggt caacgtgggg cgcggtgtga ttgtggatgt gggcggcggg
     1081 gtgtagttca cagcaatgga tgtataaaca ataggcgtag ggaggacagt ctctgcggca
     1141 gggcggatga aaaaaaacag agaaataaac cctgctagga caaggaaaag taaaacggga
     1201 acgacaccaa gcctgcgagt aggtttcggc tttggaccag agatggcggc aaggcgttct
     1261 tctagtggaa tggcatctgc gctgacggag gtgggcttgg gtggcggaag aaaggtccac
     1321 gtgttgagag cggctgagat aacgggagca aattcgggcg tagtatgaag cggaacttta
     1381 tccaatgaag tttgcgcccc taatgcaagt gaagatgcta attcagtcgt cgttttgagt
     1441 ctgtcttctg ggtttttgcg caacgcccaa aggatcatgc gtgaaaagtt atcaggtagg
     1501 ttgtggttga tgctggcagg ggcaggtggt gtcacgtcca gatgtgtttt gcgaatggca
     1561 tcactggttt tcggagcggc tttgccgttg atccagaatg aggttgttag ttcgtaaagg
     1621 atgacggcga gtgagtagat atcggcggca gtggtgagtt tttcttcgcg cagttgttcg
     1681 ggtgatttat agaacgcctg atatttgctg aacttgattt cggatttggt attgacaggt
     1741 tttgcacctg ccaacccgca gagaacgacc tcaccgcgtt tgttgatgcg aatgagttct
     1801 ggtgcaagat gcaagtgcaa taggttctgt ttgtgaagta cttcaagccc ggcgcagaca
     1861 tattttgtga tgaacaaggc ttcgtttgca cccagcctgc ctttgccccg caggtctttt
     1921 agtgaaggtc catctaccca atcttccagc agaaatgctt gttcgggagt cttttgcaga
     1981 cccagatgtt tgttgaggtt tgggtggtgc aaagtttgta aacgaacatt gttggcttca
     2041 agttctttga gtgtttccgg gtcgcttgca attgctttgg atagaatcgt aagcgcaacg
     2101 gacttcccgt tgcgctcgtc gatggcacga tataaatctc caaagggggt taaggtgatg
     2161 aactcttcaa ttcgatattg gttgagcagg gttttgatcg taacggaaga ggttgacatt
     2221 gaagtacgtc gcgatcaggt gtaggtttag agcgcccaag ccaacatcaa
//
LOCUS       DAOQFT010000421         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_31632_length_2254_cov_5.837653, whole genome shotgun sequence.
ACCESSION   DAOQFT010000421 DAOQFT010000000
VERSION     DAOQFT010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31632_length_2254_cov_5.837653"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLR93_10235"
     CDS             complement(<1..564)
                     /locus_tag="PLR93_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27389.1"
                     /translation="MKFKNLFRVAMLFATIISVFGTGAQAYAQGNSTNYLGAQIIIRS
                     TTFWDAVFNGTVNANRYERWSLQLIEPENFTISIITTSGTLTPDIYLLDASENQIASA
                     TGTLSSASLTTNQLAGDYFIQVQPHSGDGTYLMEITRTEQVVEPTASVVLNPAVIPVG
                     GTSTGTLMLSNIPSGGYASAEFTCTYNP"
     gene            complement(837..1721)
                     /locus_tag="PLR93_10240"
     CDS             complement(837..1721)
                     /locus_tag="PLR93_10240"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNH27390.1"
                     /translation="MRAWIKSTWFWLGIVALFFIAVGVYVISMLGRPPAFPTKYELYP
                     GVTYHRVVRYFPHAMIAHVVVIDRKEAKASFLVTPPDQVEGGTVKARTTSEFLKEFGV
                     QIAINGDGFSPWWSRSPVDYYPHQGDPVTPLGFAAAFGKIYSDGLLQPTQEPTLYISR
                     RNELSFNRAPGKLQHAISGDRMLLLSGEVVPDLDDTTLQPRTAIGTNRNGRYVILVVV
                     DGRQPFYSTGATFKELAELMKLYDAFFAMALDGGGSSTLVVQKKNGGTQILNSPIDNY
                     IPGRERPVANHIGVSIAP"
     gene            complement(1806..>2254)
                     /locus_tag="PLR93_10245"
     CDS             complement(1806..>2254)
                     /locus_tag="PLR93_10245"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNH27391.1"
                     /translation="YIWSIGFMPDIRRVFQYHGAEHKTINAFEAGAELTPEVVAKYPI
                     EHPRCGTAFLLTLVLLSILVFTLLGPMPIVWRLVTRVLFIPILAGIAVEYIRWTANHL
                     NNPIVQWLIKPNLALQLLTTRPPDLEMLEVAIASFQAMRKAEAETA"
BASE COUNT          638 a          550 c          535 g          531 t
ORIGIN      
        1 ggggttatag gtacaagtaa attctgcgct ggcatatccg ccactgggga tattgctcaa
       61 catcaaagta cctgtggagg ttccgccaac aggaataacc gccgggttca acaccacgga
      121 tgcggtcggt tcaaccacct gctcggtgcg ggtgatctcc attaaataag taccatcccc
      181 agagtgcggc tggacttgaa tgaagtaatc ccctgcaagt tgattagtgg tcagggaagc
      241 gcttgaaagc gtacccgttg cagaagcgat ctggttctcg ctggcatcta acaagtagat
      301 atcgggagtg agcgtgccag atgtggtgat gatcgagatg gtgaagttct caggctcgat
      361 caactgcaat gaccagcgct catagcgatt cgcatttacc gtcccattga agactgcgtc
      421 ccaaaaggta gtgctgcgaa taatgatctg tgcgccaaga taattggtgc tgtttccttg
      481 cgcgtacgct tgtgcacccg tgccaaagac actgatgatg gttgcgaaca gcatcgcaac
      541 gcgaaacaaa ttcttaaatt tcatcgtatt ctccttttcg aatcaaggtg atataaggca
      601 gaaagcctac ataacaaaaa agggtatcgt ttcgtatttc ctccaaactg ccaaccgtgg
      661 cggttcaatg atgaagactt aaaagccaat tacattctat tggtgcaact gcacaatatg
      721 cgttgcaggt gagttgccac gatattgcag aatagttgcg acatgtctgc ttaagaatca
      781 agacccactt cacatgaagt gggtcttgaa aaatcaattt aaaaatctat ttttaattac
      841 ggtgcaatcg agacaccaat atgatttgca acaggacgtt cacgccccgg aatatagtta
      901 tcaataggtg aattcaatat ttgcgttcca ccatttttct tttgcacaac caatgtagaa
      961 gacccacccc catctaatgc catggcaaag aaggcatcat ataatttcat cagctcagcc
     1021 agttctttga atgtggcacc agtactataa aacggctggc gaccatccac cactacgaga
     1081 atcacataac gcccattgcg attcgtaccg atggctgtgc gtggctgtaa agttgtatca
     1141 tccaaatctg gcacaacctc tccactaaga agcaacatgc ggtctcctga aatagcatgt
     1201 tgcaatttcc ccggcgcgcg attaaacgat aattcgttac gacggctgat gtatagcgtt
     1261 ggctcttgtg taggttgcag caacccatct gagtagattt ttccaaacgc ggcagcaaac
     1321 cccaagggtg tgactgggtc tccctgatgc ggataataat ccaccgggct gcgagaccac
     1381 catggagaga agccatcgcc attgatagcg atctgcaccc cgaactcttt caaaaattca
     1441 gaagtggtac gcgcctttac ggttccaccc tcgacctggt ctggcggcgt gaccagaaaa
     1501 cttgccttcg cctctttccg atcaatcaca accacatgcg cgatcatggc atgtggaaaa
     1561 taacgcacca cgcgatgata agtcacaccg gggtaaagct catactttgt tgggaaagcc
     1621 ggggggcgcc ccaacatgga gataacatat acccctacag cgatgaagaa caacgctact
     1681 attcccagcc aaaaccacgt agattttatc catgctctca tgtggaaaat gctatcaagc
     1741 caagatgaag aaaaaataaa aagagacggg cgaaaacccg tctctttctc tacgatagat
     1801 acactttacg ccgtttcggc ttctgccttg cgcatggctt ggaaggaagc aatcgccact
     1861 tcgagcattt ccaaatctgg cggacgggtg gtgagcaatt gcagtgccaa attcggcttg
     1921 attagccatt gcacaatggg gttattcaag tgattcgccg tccagcggat gtattcgacg
     1981 gcaatgcccg ccaaaatagg gatgaacaaa acgcgggtga ccaaacgcca aacgatgggc
     2041 ataggaccta acaaggtgaa cacgagaatg gagagcaaga ccaaagtcaa caaaaaggcg
     2101 gtgccacagc gcggatgttc aatgggatat tttgccacca cttctggagt caactcagcg
     2161 ccggcttcaa aggcgttgat ggttttgtgt tcggcgccat gatattgaaa gacacggcga
     2221 atatcgggca tgaaaccaat gctccagata tacc
//
LOCUS       DAOQFT010000422         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_31663_length_2253_cov_6.376251, whole genome shotgun sequence.
ACCESSION   DAOQFT010000422 DAOQFT010000000
VERSION     DAOQFT010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31663_length_2253_cov_6.376251"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLR93_10250"
     CDS             <1..514
                     /locus_tag="PLR93_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27392.1"
                     /translation="YAVSSSTSFGRAYYQPQFNRVGEVIQERDPITGASVSAKLESSS
                     PAYHITYTAALNSGETFNGSETILGTTIGLRGLGMPAPSKFTFKAADYEAEFTGTLTS
                     ELALSLFGNTKIRAYGSLDFKDNAGNAGHLDLNRQGYVTLKINDQPTVTHALVRVMWL
                     DMMSLSPQPL"
     gene            511..1857
                     /locus_tag="PLR93_10255"
     CDS             511..1857
                     /locus_tag="PLR93_10255"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNH27393.1"
                     /translation="MTNIWWIRRDLRLTDNAALHSALEAGSVLPVFILDPAFDNLSER
                     RKHFLYEGLSALDKDLHKRNSYLVVRKGKPIDALRQLFDEVKAETIYAEEDYTPYSRK
                     RDEFIQKLLPLSLVQGQTVHHPKSVLKQDGRPYTVYTPYSKVWKAQLTKIKLHPAPEN
                     LNTPKSIRSEPLPTFTPNPIFPAGEAEALRRLNQFTQSIIKNYHDDRNRMDLDGTSSL
                     SPYIRFGMVGLRQAVHAARATHHADSKGAEIWLNELIWREFYIQILYHFPYVAKTAFN
                     RSLANIPWRNKSSEFEAWKRGETGVPVVDAAMRQLKETGWMHNRARMIVASFLVKDLL
                     IDWRWGEDWFMQNLLDGDIAPNNGGWQWTAGTGTDAAPYFRIFNPVLQSAKFDPNGDY
                     IRKWVPELRGLSAKQIHAPWEYDFKISGYPSEPIVDRDVVKERTLNAYSVSKEKIR"
     gene            1854..>2253
                     /locus_tag="PLR93_10260"
     CDS             1854..>2253
                     /locus_tag="PLR93_10260"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HNH27394.1"
                     /translation="MNKRDLSVSMERANIIVLFIGIPVVILQFILFDQLHPSPAETGE
                     WNFLLLLLVIVMGVILHEIVHGVTWAIAGRKPWTSIKFGFQAKTLTPYCHITEPLEIN
                     AYRIGAVMPGLVVGILPYVYSLMSGDMNWLW"
BASE COUNT          579 a          576 c          532 g          566 t
ORIGIN      
        1 gtatgcagtt tcatcttcca cttcgtttgg gcgggcgtat taccagccac agttcaaccg
       61 cgtaggagag gtgattcaag agcgcgaccc catcacaggc gcatcggtat ctgccaagct
      121 tgaaagcagt tcacctgctt atcacattac ctacacggca gccttgaaca gcggcgaaac
      181 cttcaatggt tctgaaacca tcctcggcac tactatcggg ctgcgcggtt taggcatgcc
      241 tgccccatcc aagttcactt tcaaagctgc agactatgaa gcagaattca caggcacact
      301 taccagtgaa cttgcccttt cccttttcgg caataccaag attcgtgctt acggctcgct
      361 cgacttcaaa gataacgcag ggaacgccgg gcatcttgac ctaaaccgtc agggctacgt
      421 cacactaaaa atcaacgatc aacccaccgt cacacacgct cttgtgcgcg tgatgtggtt
      481 ggatatgatg agcctttctc cgcaaccgct atgacaaata tttggtggat tcgccgcgac
      541 ctacgtttga cagataatgc cgcgcttcat tccgcacttg aagctggttc tgtcttgccg
      601 gtctttattc tcgacccggc attcgacaac ctatcagaac ggcgcaaaca ttttctgtat
      661 gaaggcttat ctgctttaga caaagacctg cataaacgta attcatattt agtcgttcgc
      721 aagggaaaac ctatcgatgc actccgtcaa cttttcgacg aagtaaaagc tgaaaccatc
      781 tatgcggaag aagattacac accctattct cgcaaacgag acgaatttat tcaaaagctg
      841 ttgcctttga gtttggtcca agggcaaacc gtacatcacc ctaaatcggt cctcaagcaa
      901 gatggtagac cttacacggt gtacacaccc tattccaaag tatggaaagc acagcttacc
      961 aagatcaaat tacatcctgc accagaaaat ctgaatacac ccaaaagcat tcgttctgag
     1021 ccgctaccta cattcacgcc gaatcccatt ttcccggcag gcgaagcgga agcgctccgc
     1081 cgccttaacc aatttactca atcaatcatc aagaattacc acgacgaccg caaccgtatg
     1141 gacttggatg gaacctcatc tctctcacca tacatccgct ttggcatggt tgggttgcgt
     1201 caagccgtgc atgctgcacg agctacacat cacgcagatt ctaaaggcgc agaaatctgg
     1261 ctcaatgaac tcatctggcg tgagttctac attcagattc tgtatcactt cccgtatgtt
     1321 gctaaaaccg ccttcaaccg ctcactggcg aacattccgt ggcgcaataa atcgtcagag
     1381 tttgaagcgt ggaaaagggg cgaaacaggc gtcccggtgg tagatgcggc gatgcgtcag
     1441 ctcaaagaaa cgggttggat gcacaaccgc gcgcgcatga tcgtcgcatc gttccttgtc
     1501 aaagacttgc tcattgattg gcgctggggc gaagattggt tcatgcaaaa cctgctcgat
     1561 ggcgatatcg cacctaacaa cggcggctgg caatggacgg cgggaacggg caccgacgcc
     1621 gcgccgtatt ttcgtatttt taacccggtg ctgcaaagtg cgaagttcga cccgaacggt
     1681 gattacattc gcaaatgggt gccagagttg cgcgggttaa gtgcgaagca aattcacgcg
     1741 ccgtgggaat atgatttcaa gatatcgggg tatccatctg aaccgatcgt agatcgagac
     1801 gtggtaaaag agcgcacgtt gaacgcatat agtgtttcaa aggaaaaaat acgatgaaca
     1861 aacgtgatct atctgtttcg atggaacgcg caaacatcat tgtgcttttc atcgggattc
     1921 ctgtagtgat tctacaattc atcctctttg atcaattaca cccatcgccc gctgaaactg
     1981 gtgaatggaa tttcttgttg ttgctcttgg tcattgttat gggtgtgata cttcatgaga
     2041 tcgtgcatgg ggttacctgg gcaattgccg ggcgtaagcc ttggacttcg atcaagttcg
     2101 ggtttcaagc caaaacgctt acaccgtatt gccatataac tgagccgctt gagattaatg
     2161 cgtatcgtat tggtgcggtc atgccggggt tggtcgttgg gattctgcct tacgtctata
     2221 gtcttatgag tggagatatg aactggttat ggt
//
LOCUS       DAOQFT010000423         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_31667_length_2253_cov_4.845314, whole genome shotgun sequence.
ACCESSION   DAOQFT010000423 DAOQFT010000000
VERSION     DAOQFT010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31667_length_2253_cov_4.845314"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLR93_10265"
     CDS             complement(<1..204)
                     /locus_tag="PLR93_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27395.1"
                     /translation="MENELYPKSIFFGTWLGAVIGTLLGAGSGAISGTWNGILTAAIM
                     GLVTGAINGALTGAVVVRTTGTTG"
     gene            369..1004
                     /gene="folE"
                     /locus_tag="PLR93_10270"
     CDS             369..1004
                     /gene="folE"
                     /locus_tag="PLR93_10270"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNH27396.1"
                     /translation="MIDPETNEDFDERPTFDEEINTVAIEESIRAMLTAFGENPDREG
                     LQNTPKRVARMYPELLSGYRTDVDKLVNGAIFNVTYDDMVIVRDIEYFSLCEHHMLPF
                     MGRAHVAYIPSGQVIGLSKIPRIVDMFARRLQVQERMTRQIADFIHQLLKPQGVAVVV
                     EGLHLCAMMRGVKKHDARMTTSAMLGSFRKSINTRQEFLDHLNRGAEPLRI"
     gene            complement(973..1500)
                     /gene="folK"
                     /locus_tag="PLR93_10275"
     CDS             complement(973..1500)
                     /gene="folK"
                     /locus_tag="PLR93_10275"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNH27397.1"
                     /translation="MSANEMSDLHHVYLDLGSNLEPEKNIPLAIELLQNVGEVTAVSS
                     VWETESVGYAGPNFLNVCVLLRTSLDAEQVKHEINRPIEAQMGRVRGEDKYVPRPIDI
                     DIVLFDETPHNVQTWNQAFVIVPLAELVPEFKPHSGETLSELAKQLQSQVWMRKREDV
                     IYPTGTRSVAVPRRG"
     gene            complement(1478..1834)
                     /gene="folB"
                     /locus_tag="PLR93_10280"
     CDS             complement(1478..1834)
                     /gene="folB"
                     /locus_tag="PLR93_10280"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNH27398.1"
                     /translation="MDKVFIKDLLVRGIIGIREWEREKEQDILINVTVYTDTIRAAES
                     DDINDCVDYSALAKKVQHHAETVQRLTVEALANDLATICLETPLVRKVVVRVEKPGAV
                     RFAKSVGVEIERKRDE"
     gene            complement(1966..>2253)
                     /locus_tag="PLR93_10285"
     CDS             complement(1966..>2253)
                     /locus_tag="PLR93_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH27399.1"
                     /translation="GADHFPYTVTKSALAAMTKSLAIALAPNITVNGLALGAILPPAD
                     NPSASEKIIEAVPAKRWSEANEVEEALLFLLSGPAYITGEILHVDGGRHLV"
BASE COUNT          546 a          605 c          541 g          561 t
ORIGIN      
        1 gcccgtcgtc cctgtggtgc ggacgacaac cgccccggtt aaagctccgt tgatggcgcc
       61 ggttacaagc cccatgatgg ctgcggtgag aatcccattc catgtgccgc tgatcgcacc
      121 cgagccagcc ccaagaaggg tgccaatgac ggcacccaac caggtgccaa agaaaatgct
      181 ttttggataa agttcgtttt ccatagttat ctccacattt gatactgtat tttacctttt
      241 cagccaattg gtaagggcga cccttccctg atagtaacgt acatttgctt tcactgggcg
      301 ggtcgcccct acactgtgta aagataaatc agatacaatc ggcatacccc accataatcg
      361 gagatgcgat gatagacccc gaaaccaacg aagattttga tgagcgccca acctttgacg
      421 aagaaataaa tacagttgct atagaagaat ccattcgggc gatgctcact gcttttggtg
      481 aaaacccaga ccgtgaaggc ttgcaaaata cacccaagcg tgtagcacgc atgtaccccg
      541 aactgttaag cggttaccgc accgatgtgg ataaactcgt caacggcgcg atcttcaacg
      601 tgacctatga cgacatggtc attgtgcgcg atattgaata ctttagtttg tgtgaacatc
      661 acatgctgcc atttatgggg cgtgcgcatg tggcgtatat tccaagtggt caggttattg
      721 ggctgtcgaa aattccccgc atcgttgata tgttcgcccg acgtttacag gttcaggaac
      781 gcatgacccg tcagatcgcg gactttatcc atcaattact taaacctcaa ggtgtggcag
      841 ttgtggtcga aggtttgcat ctctgcgcga tgatgcgcgg cgtgaaaaag catgatgccc
      901 gcatgacgac ctccgccatg ctgggatcgt tccgcaagag tatcaataca agacaagaat
      961 tcttggatca cctcaaccgc ggcgcggaac cgctacggat ctagtgcccg tagggtatat
     1021 aacatcttct ctctttcgca tccatacctg actttgtaac tgcttcgcta attctgataa
     1081 tgtttccccg ctgtgaggtt tgaactccgg cacaagttct gccaatggca caatgacaaa
     1141 tgcctgattc caagtttgga cgttgtgcgg cgtttcatcg aataaaacaa tatcaatatc
     1201 aattgggcgc ggaacatatt tatcctcgcc gcgtacacgc cccatctgtg cttcgatggg
     1261 acgattgatt tcatgcttga cttgttctgc atccagagat gtccgcagca ggacacagac
     1321 atttaaaaaa ttgggtccag catagcccac cgactctgtc tcccacaccg aagagacggc
     1381 tgtaacctca cccacatttt gcaacaactc aatagcgagc ggaatattct tttctggctc
     1441 aaggttcgag ccaagatcga gatagacgtg gtgcaggtca ctcatctcgt ttgcgctcaa
     1501 tctctactcc cacggacttg gcaaaacgta ctgcgcccgg tttctccacg cggacgacca
     1561 ccttacgcac caagggcgtc tcaagacaga tcgtcgccag gtcatttgcc agcgcttcaa
     1621 ccgtaagtcg ctgaacagtt tcagcatggt gttgaacctt tttcgccagc gcgctgtaat
     1681 ccacgcagtc gttgatatcg tcagactcag cggcgcgaat cgtgtcggta tagaccgtca
     1741 cgttgatgag aatatcctgc tctttctcac gctcccactc gcggataccg ataatgccac
     1801 gcacgagcag gtctttgata aatactttgt ccatgagggc tcctaatcta attcatcgtc
     1861 attgcgaggg cgatgcactt tcgcccgaag caatctcatg acactgtatg cgagattgct
     1921 tcgtcgcgaa gaacaagagc gctcctcgca atgacggaat ggtaattaca ccaaatgcct
     1981 gccgccatcc acatgcaaaa tctcgccggt gatataagcg ggacctgaca ataaaaacag
     2041 cagcgcttct tcgacttcgt tcgcctcact ccatcttttg gctggtaccg cctcgatgat
     2101 cttttcactc gcagacgggt tatctgcggg tggcaggatg gcgcccaacg ctaatccatt
     2161 tacagtgatg ttcggtgcaa gcgcgatggc tagtgatttg gtcatcgctg ctagcgcaga
     2221 tttggtgacg gtgtagggga agtggtccgc gcc
//
LOCUS       DAOQFT010000424         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_31688_length_2252_cov_5.144288, whole genome shotgun sequence.
ACCESSION   DAOQFT010000424 DAOQFT010000000
VERSION     DAOQFT010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31688_length_2252_cov_5.144288"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLR93_10290"
     CDS             <1..580
                     /locus_tag="PLR93_10290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNH27400.1"
                     /translation="QAGASGLPFLPMNQTGAEDLEKVNPQIKRIPDPYGGKDVIVVPA
                     LNPDVAIVHVQRADKNGNAHLWGIIGEQKEAAFAAKKVILTAEEIVDETVIRSDPNRT
                     MIPGIVVSAVCHVPFASHPSYSQGYYDRDNEFYLAWDKISESKELTQKYLDEWVYGVK
                     DRNAYWKKLGEKTHKRLKVKAQYSEKINYGKY"
     gene            582..1430
                     /locus_tag="PLR93_10295"
     CDS             582..1430
                     /locus_tag="PLR93_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH27401.1"
                     /translation="MNPIPHFDFGGNGEPLHFLHANGYPPECYTSLLEHLKHNYHLFG
                     MKLRPLWDGANMDDLHDWHPYSEDLLRLLSDRETGPVIGVGHSIGGIVTLRAAMHEPK
                     RFRAVILLDPVLFVPSTMNIWNFVRAIGIGEKAHPLIQTAKRRRREFTDLESVFRSYR
                     TKPVFRYMSDESLKQYIVGITKPKASDGYELVYTPEWETHIYMTGMRDYDLWRGLANF
                     DVPTLIIRGDETDTFLPNAEKLVKKKNPKIQIHTMKKATHILPLEHPKEVADIIKNFL
                     TTDSRS"
     gene            1468..2004
                     /locus_tag="PLR93_10300"
     CDS             1468..2004
                     /locus_tag="PLR93_10300"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNH27402.1"
                     /translation="MPELKYSSAELMIINAARLLRDGDVVFVGVGQPNLACNLAKRTH
                     APNLVMIYEAGVIGAEPERLPLSIGDPTLVSGALSVVSMYDIFANYLQRGNVDVGFMG
                     GAQIDKYGNINATTIGDYAHPKVRLPGSGGSQEIAAWANRCYIMTPHQKRRFPEKVEF
                     MTSAGFIDGKGAREARGI"
     gene            complement(1964..>2252)
                     /locus_tag="PLR93_10305"
     CDS             complement(1964..>2252)
                     /locus_tag="PLR93_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27403.1"
                     /translation="GKASRKMLKYGYLQLQKNPNDVDAMYAMASGIINLGYMSGMSYQ
                     KSYVEEALLRLNRAAALNSKSQNKILKRRAQAYIYLGDFKSHALRVLLCHQ"
BASE COUNT          622 a          537 c          515 g          578 t
ORIGIN      
        1 acaggcaggt gcatctggct tgccattcct gcccatgaat caaaccggtg cagaggactt
       61 ggaaaaggtt aatccgcaga tcaagcgcat ccccgaccca tacggcggaa aagatgtgat
      121 tgtcgtgcca gccttgaatc cagatgttgc cattgtccac gtacagcgcg cagataagaa
      181 tggcaatgcc catctttggg gcatcatcgg cgaacagaaa gaagccgctt tcgccgcgaa
      241 aaaagtcatc cttaccgctg aagaaattgt cgacgaaaca gttatccggt ctgacccgaa
      301 ccgcacgatg attcccggca ttgtcgtcag cgcggtatgt cacgttccgt tcgcgagtca
      361 tccatcgtat tcgcaaggtt attacgatcg cgacaatgag ttctaccttg cgtgggataa
      421 gatcagcgaa tcaaaagagc tgactcaaaa atatcttgat gaatgggtgt atggcgtaaa
      481 agaccgcaat gcctattgga aaaagctcgg cgaaaagaca cacaagcgtt tgaaagttaa
      541 agcacaatat agtgaaaaaa taaattacgg caagtactaa cgtgaatcca attccacatt
      601 ttgattttgg cggcaatggt gaaccgctgc attttcttca tgccaatggc tacccgccag
      661 aatgttacac atcactactg gaacatttaa aacataacta tcatctcttt ggaatgaaac
      721 tccgtccact atgggatggc gccaatatgg atgacctgca cgattggcac ccatactcag
      781 aagatctact gcgcctctta tcggaccggg aaactggtcc tgttattgga gtgggtcatt
      841 ccatcggagg gattgtcacc ctgcgagctg ccatgcatga gccaaagcga ttccgcgctg
      901 tgattctgct tgaccctgtg cttttcgttc ccagcacaat gaatatctgg aattttgtcc
      961 gcgccatagg gattggagaa aaagcccatc cgcttattca aacagccaaa cggcgcagac
     1021 gtgaattcac agatttggaa agtgtctttc gcagttatcg aacaaagccc gtttttcgct
     1081 atatgagtga tgagagtttg aagcaataca ttgtaggcat caccaagccc aaagcaagcg
     1141 acgggtatga gctcgtttat acccctgaat gggaaactca catttatatg accggaatgc
     1201 gcgattacga cctctggcgc gggcttgcaa attttgatgt accaacattg attattcgtg
     1261 gtgatgaaac cgataccttc ctgccaaatg ctgaaaagct ggtaaaaaag aaaaacccga
     1321 agattcaaat tcacaccatg aaaaaagcca ctcacattct gccgcttgaa catccaaaag
     1381 aagtggctga cattattaag aactttttga cgacagacag tagatcatag gcgagccatt
     1441 gtctatgatt caatttagga gaacaacatg cccgagctca aatattcctc cgctgaactg
     1501 atgatcatca atgccgcccg cctcttacgc gacggcgatg tggtcttcgt tggtgtaggt
     1561 caacccaatc ttgcttgtaa tcttgccaag cgtacccatg ctccgaacct tgtaatgatc
     1621 tatgaagccg gcgtgatcgg tgctgaacca gaacgcctgc ccctctccat cggtgacccg
     1681 acgctggtca gtggtgcgct ttcggttgtg agtatgtatg atatttttgc caattacctt
     1741 cagcgtggca atgtagatgt aggttttatg ggtggtgcgc agattgataa gtatggaaac
     1801 atcaatgcca caacaattgg tgactatgcg catccaaaag tccgcttacc gggttcgggt
     1861 ggttcacagg aaattgcagc atgggcaaat cgctgttaca tcatgacccc tcaccagaag
     1921 cgacgtttcc ctgagaaagt ggagttcatg acctcggctg gttttattga tggcaaagga
     1981 gcacgcgaag cgcgtgggat ttgaagtcgc ccaaataaat ataggcctgc gctcttcgtt
     2041 ttagtatttt attctgcgac ttcgaattca aagcggccgc cctatttaaa cgcaataaag
     2101 cttcttcaac atatgatttc tgataagaca tgccgctcat atatcctaaa ttaattattc
     2161 cactagccat cgcatacatg gcatcaacat cgttcggatt tttttgcaat tgcagatacc
     2221 catactttag catttttcgt gaagcttttc cc
//
LOCUS       DAOQFT010000425         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_31759_length_2249_cov_4.616682, whole genome shotgun sequence.
ACCESSION   DAOQFT010000425 DAOQFT010000000
VERSION     DAOQFT010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31759_length_2249_cov_4.616682"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLR93_10310"
     CDS             <1..332
                     /locus_tag="PLR93_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNH27404.1"
                     /translation="YENTYPDIVSWWWSYRIDFYKPEGFVDIPIYEGQGFRPYTNAAY
                     FQGAHFLEKVRERMGEEAFFAFLQDYYNQGRGKIVTANDFFRILRSHTSADLSDLINQ
                     YFRSIYP"
     gene            329..1891
                     /locus_tag="PLR93_10315"
     CDS             329..1891
                     /locus_tag="PLR93_10315"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNH27405.1"
                     /translation="MKKNSRITFHFLLSTLLLSCAAPTPTATPQPFAPFIMPTRVTLV
                     TATPFQPVIQTDTPIPTYTASPIPSTTPTATLTPPPTTPVTAATTQAPPPPTNSSRPN
                     YILFATLDFANKTITADQTIRYYNNTGTTLSDLVLSVQPNTYTGNFSLTALAQDGTAL
                     TSYTLSGQRLTINLNQAISSGTATTLTLTFTLNIPQKSSGNVFGYDFNQINLVNWYPF
                     IVPYRGGWILHDPMPWGEHLVYDSSDIELNIKTDSGVTLAVGASPEPNGEWTRYRMYG
                     ARTMAISASNEFIVNETTVGNVVIRSYYFSGFQSGGDDMLIYGSEAVETYSTQFAPYP
                     HQTLAIVQSDMDDGMEYDGLVFLSTEFYSQYNGTARSNLAVIGVHEIAHQWWYGLVGS
                     DHALEPFLDEALATYTERIFFENNYPSSVSWWWQFRVDYFDPSGYVDGSIYDYGSFRD
                     YTDAVYFNGAHFLDEMRELMGFGNFSKFIKAYALRYGYGHATIADFFALAREIVNINY
                     DGLIEKYFLGSY"
     gene            2027..>2249
                     /locus_tag="PLR93_10320"
     CDS             2027..>2249
                     /locus_tag="PLR93_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH27406.1"
                     /translation="MNRPYTFINTAMTADGKIDTFERKGAAISSKRDKQRVDELRAAA
                     DAVLVGGKTLLEELPKLTVKSEALREGRIR"
BASE COUNT          560 a          710 c          453 g          526 t
ORIGIN      
        1 tctacgaaaa cacctacccc gacatcgttt cttggtggtg gtcataccga atcgactttt
       61 acaaacccga aggcttcgtt gatattccca tctacgaagg gcagggcttc cgtccctata
      121 ccaatgccgc ctacttccaa ggtgcgcact tcctcgaaaa agttcgcgaa cgcatgggcg
      181 aggaagcctt cttcgccttc cttcaagatt actacaacca gggccgcggc aagatcgtca
      241 ccgccaacga cttcttccgc atcctgcgct cacacaccag cgccgacctc tctgacctca
      301 tcaaccaata cttccgcagc atctatccat gaaaaagaat tcacgcatca ctttccactt
      361 tctactttcc accctcctcc tctcctgcgc cgcgccaacc cccaccgcaa cgccacaacc
      421 cttcgcgccc ttcatcatgc ccacacgcgt gaccctcgtc accgccacac ccttccaacc
      481 cgttatccag acagacacac ccattccaac ctacacggcc tccccaattc ccagcacaac
      541 acctactgcg acgttaaccc cgcctcccac cacaccggtc actgccgcca ccacccaagc
      601 cccccccccg ccgacaaatt catcgcgccc aaattacatc ctctttgcca cactcgactt
      661 tgccaataaa accatcaccg ccgatcaaac gattcgatac tacaacaaca caggcacaac
      721 tctttccgat cttgtcctat ctgttcagcc aaatacctat acaggcaact tcagcctcac
      781 tgcacttgcc caggatggaa cggctctcac ctcctacacc cttagcgggc aacgcctcac
      841 catcaacttg aatcaagcca tatcttccgg gacggcaacc accctcacac taacctttac
      901 cttgaacatc ccacaaaaat catcgggcaa tgttttcggt tacgacttca accaaattaa
      961 cctcgtaaac tggtatccat tcatcgtccc ctaccgcggc gggtggattc tgcacgaccc
     1021 gatgccctgg ggagaacatc tcgtctacga ctcatccgac attgaactaa atatcaaaac
     1081 cgactctggc gtgactctgg ctgtgggcgc ttcgcccgaa cccaacggcg agtggacacg
     1141 ttaccgaatg tatggcgcac ggacgatggc aatctctgcc agcaatgaat tcattgtcaa
     1201 cgagacaaca gtcggcaatg tcgtcatccg ctcctattat ttcagtggct ttcaaagcgg
     1261 tggcgatgat atgctcattt acggcagtga agctgtcgaa acgtattcca cgcaatttgc
     1321 accctatccg catcaaactc tcgctatcgt gcaatcagac atggacgatg gcatggaata
     1381 tgatggtctg gtctttctgt ccaccgaatt ttacagtcag tacaacggca cagcccgcag
     1441 caatctggcg gtcattggtg tgcatgagat cgcgcatcaa tggtggtacg gtctggttgg
     1501 cagtgaccac gccctcgaac ccttcctgga tgaagcgctt gctacataca ccgaacgcat
     1561 cttcttcgaa aataattacc ccagtagtgt cagttggtgg tggcaattcc gcgtggatta
     1621 ctttgacccc tccgggtatg tggatggctc gatctacgac tacggctcct tccgagatta
     1681 caccgacgcc gtctatttca acggcgccca ctttctggat gaaatgcgcg aactgatggg
     1741 ctttggtaat ttttcaaagt tcattaaagc atacgccttg cgatatggct atggtcacgc
     1801 caccattgca gatttcttcg cccttgcgcg tgaaattgtg aacatcaatt atgatggttt
     1861 gatcgaaaaa tactttttgg gttcatacta actttggcaa ttcccactct cttacttttg
     1921 ggggttggat aaaccagccc gcaaacccta taatcatgct gtcttatttt agacagcatt
     1981 ttcttttgta agaagcgcac aactcaacaa gtcaaagaac aaattcatga accgacccta
     2041 caccttcatc aataccgcca tgacggcaga tggcaaaata gatacgtttg aacgaaaagg
     2101 tgcggccatt tcttccaaac gcgataaaca acgcgtggat gagttacgcg ccgctgccga
     2161 tgcggtcctc gtcggcggga aaacactttt ggaggaactg cccaagctta cggtcaagag
     2221 tgaggcgctg cgtgaggggc ggatcaggc
//
LOCUS       DAOQFT010000426         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_31762_length_2249_cov_3.954877, whole genome shotgun sequence.
ACCESSION   DAOQFT010000426 DAOQFT010000000
VERSION     DAOQFT010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31762_length_2249_cov_3.954877"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLR93_10325"
     CDS             <1..930
                     /locus_tag="PLR93_10325"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNH27407.1"
                     /translation="ASNATGPSSFTVTFSEDVSNTGGGSLIDDVTNINNYKIINKGAN
                     GTVDTASCASAIGGDDSIILPSNVTYGPNTAVVTLGTLPTGNYRLFVCGTTSIVDLAG
                     NPLAGDGVTSGTDFTFDFTVGAVTATTVNTSASSLPKTGFAPNKITSIPAQPAELAYA
                     ELGDLWLEIPLLNVKSTIVGVPQNANKTWDVSWLGNDTGWLNGTAFPTWTGNSVLTAH
                     VTNASGLDGPFAALKSLKYGDQVIVHLGGVKYVYEVRVTKLARPYTTNFTFESKQDAS
                     YLTLVTCSGYNPLNESYLFRRIVRAVLVSTVSE"
     gene            complement(983..>2249)
                     /locus_tag="PLR93_10330"
     CDS             complement(983..>2249)
                     /locus_tag="PLR93_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27408.1"
                     /translation="QWAKETDKTAFLQLTSSLWLFFRSLEHKDEGIDCLLKAVSANKD
                     TPTPLLSTAMARLASLSIYLSIVQDQTEDYALTALDLSRQVRDKPAEALALISLAGLE
                     LERMTGKFGPEYTELALGIARDLKDSWLISTALIQKGRFTQIKNPIESVAIFEEALKE
                     AQASGDKRLLYSNLFWMFINILATGQLARAKEIAHQCVLVATEIGDKDGIMYSHNALA
                     GIGLFEEDYESSKEHAETIIRLSKAYQHNSGLLNGLGTAGLVNLATKNISQVLELSTE
                     MDRLILSKSGRLKSDTGYSVFLRIWAYILERDLKNARSNARELIPLYTQNDNILLSIE
                     YFRVFAALAYMDGCHEINITLTSFAKRLHERVALAYFDYPFMSRLRAEQLAQSREALG
                     ADAFNQAWEKGQQMDLEEAKKYILEIANR"
BASE COUNT          563 a          593 c          508 g          585 t
ORIGIN      
        1 gccagcaatg ccacaggtcc ttcaagtttc actgtcacct tcagcgagga tgtcagcaac
       61 actggtggcg gttctttgat cgatgatgtc accaacatca ataactacaa gatcatcaac
      121 aagggcgcga atggcacggt cgacaccgcc tcttgcgcct cagccatcgg cggcgatgac
      181 tcgatcattc ttccatccaa cgtaacctat ggacccaaca ccgccgttgt cacgctcggc
      241 acgttgccca ctggcaatta tcgtctcttc gtctgcggca ccacctccat tgtggaccta
      301 gcaggcaatc ctctcgcagg cgatggcgtg acttccggca ccgacttcac ctttgacttc
      361 actgtcggtg cggtgactgc aaccacagta aacaccagcg cttcttcctt acccaaaact
      421 gggtttgccc cgaataaaat cacctccatc ccagcccagc ccgcagaact cgcctatgcc
      481 gaactcggcg acctctggct ggagattcct ttattaaatg taaagtccac cattgtgggc
      541 gtgccccaaa acgcgaataa gacttgggat gtgagttggc tcggaaatga caccggttgg
      601 ctcaacggca ccgccttccc cacttggacc ggtaactcgg tgctcaccgc ccacgtcacc
      661 aacgccagcg ggttggacgg tcccttcgcg gcgttgaagt cgctcaagta tggcgaccaa
      721 gtcatcgtcc atttgggagg cgtgaagtat gtctatgagg tgcgggtcac caagttggca
      781 cgtccctaca ctaccaactt caccttcgaa tccaagcagg atgcttcgta tctcactctc
      841 gtcacctgct cagggtataa cccgttgaat gaaagttatc tcttcagaag aatagttcga
      901 gccgtactgg tcagcacggt cagcgagtaa aacaaaaaga cctccgaagc aacttcggag
      961 gtctttttta tgagcaaggg cgttatcggt tggctatttc caatatgtat ttttttgcct
     1021 cttccaagtc catctgctgt cctttttccc aagcttgatt aaaagcatcg gctcccaagg
     1081 cttcgcgtga ttgggctagt tgttctgccc gcagcctgct cataaatgga taatcgaagt
     1141 aggctaaagc aactcgttcg tgcaatctct tggcaaagct ggtcagggtt atatttattt
     1201 catggcagcc atccatatat gccagcgccg caaaaacccg gaagtattca atggataaaa
     1261 ggatgttgtc attctgggta tacaggggga tgagttctct ggcattactt ctggcgtttt
     1321 tcagatcacg ttcaaggata taagcccaga tgcgaagaaa cacagagtaa ccggtatcag
     1381 acttaaggcg accactcttt gaaaggatca acctatccat ttcagtagat agttcgagta
     1441 cttgtgaaat attttttgtc gccaaattta ccagcccggc ggttccaagc ccatttagta
     1501 gaccagaatt gtgctgataa gcttttgata atcgaatgat ggtctcggcg tgttctttgg
     1561 aggattcata atcctcttca aataaaccaa tacccgccaa ggcattatga gaatacataa
     1621 tgccgtcctt atcgccgatc tctgttgcaa ccagtacaca ttgatgggca atctcttttg
     1681 ccctggcaag ctgccccgtt gccagaatat tgataaacat ccaaaataaa ttggagtaca
     1741 gcaggcgctt atcgccagat gcctgagctt ctttgagtgc ctcttcaaaa atagctacgc
     1801 tttcaatcgg attctttatt tgggtaaatc tccccttctg aatcaaagca gtggagatta
     1861 accaagaatc tttgaggtca cgtgcaattc ccaatgccag ctcggtatac tccggaccga
     1921 actttccggt catgcgttcc aattccaagc ctgccaatga aattaacgcc aaggcttcag
     1981 cgggtttatc tcttacctgc ctgcttaggt caagtgctgt aagggcatag tcttccgttt
     2041 gatcctgcac aatagaaagg tagatggaaa gagaagctag ccgcgccatg gcagtcgaaa
     2101 gtaaaggggt tggtgtatct ttgtttgccg acacagcttt caacaaacag tcaatgcctt
     2161 catctttatg ttccaacgac ctaaaaaaga gccaaaggga actcgtcaat tgaaggaagg
     2221 cggttttatc cgtttctttt gcccattgc
//
LOCUS       DAOQFT010000427         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32142_length_2232_cov_5.728525, whole genome shotgun sequence.
ACCESSION   DAOQFT010000427 DAOQFT010000000
VERSION     DAOQFT010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32142_length_2232_cov_5.728525"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLR93_10335"
     CDS             complement(<1..707)
                     /locus_tag="PLR93_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNH27409.1"
                     /translation="MIPLHLRIAGFLSYRDPVDLDFNMVDLACISGHNGAGKSSLLDA
                     FTWSLFGEARGKGVDVINLSQDVKAAEVALTFLYEGNIYRVQRTLPRNKSTVLEFQVL
                     DRGSQTTDDGRWRPLTEKTTRDTQARIEQTLRLDYETFINASFFLQGKADQFTQKKAS
                     ERKAVLSAILGLEIWDTYKDRTAERRRELETEVTEIDGRIAEIDAELGEEDERRRRLS
                     ELEESLKQLTTAREAQES"
     gene            complement(821..1174)
                     /locus_tag="PLR93_10340"
     CDS             complement(821..1174)
                     /locus_tag="PLR93_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27410.1"
                     /translation="MSVVVGVGVLVIVAVMVGVFDAVGVNVLVGVKVRVVVGVNVNVL
                     VLGRKNVSVAVGVDVDVGVEVIVGVNVIVPVTIKGVLLAVAVFGVPVTVSVMDGVGVT
                     SRGLGAKERQTNPAQ"
     gene            1198..>2232
                     /locus_tag="PLR93_10345"
     CDS             1198..>2232
                     /locus_tag="PLR93_10345"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27411.1"
                     /translation="MSDILPFLKSIISISGVSGDEAPVADLVQKKWTPLVDEISQSRL
                     GSIHGLKQGTLKKSSSKAGSASRPSVMIATHMDAIGMMVTRIVDGFIYFTNVGGIDSR
                     VLPGTPVIVHASGTKEELYGVIVMPPGNLLPEGEGGGVVPMKYLFVDTGLFAGEVSKR
                     VRVGDRISFGTEPVEMSGGTVSGHTLDNRASVAALTVCLEELQSKSHIWDLWAVATVQ
                     EETSFGGSYSSTFQLRPTIAIALDMTFGKGAGASGYQTFPLGKGVTLGIGPSVHPFLY
                     QRFKEVAERIEIPIADDLMPEYSSTDADAMQLIAEGIPTMVVSIPQRYMHTAVELVAL
                     KDIQRAGRLMA"
BASE COUNT          541 a          665 c          491 g          535 t
ORIGIN      
        1 gattcttgtg cttcgcgcgc cgtcgttaac tgcttcagtg attcttctaa ttcgcttaaa
       61 cggcgtcggc gttcgtcttc ttcccctaat tccgcgtcga tctccgcaat gcgaccatca
      121 atttcagtga cttcggtttc caattcacga cgacgttctg ccgttcggtc tttgtaggta
      181 tcccaaattt ccaacccgag aatggcgctt agcactgcct ttcgctcgct ggctttcttc
      241 tgcgtgaatt ggtcggcttt gccttgcaaa aagaacgaag cgttgataaa cgtctcataa
      301 tcgaggcgta aggtttgttc aatgcgggct tgcgtatcac gggtcgtttt ctccgtcagc
      361 ggacgccacc gtccatcgtc ggtcgtctgt gacccacggt ctaagacctg aaactccaaa
      421 accgtactct tattgcgtgg caaagtccgc tgcacgcgat aaatattgcc ttcatataaa
      481 aacgtgaggg caacttcagc agcttttacg tcctggctta ggttgataac atccacccct
      541 ttaccgcgtg cctccccaaa caatgaccat gtaaacgcat ccaataaaga agacttgccc
      601 gcgccgttat gcccagaaat acaagcaagg tctaccatgt tgaaatccaa gtcaacaggg
      661 tcacgataag agaggaaacc agcaatacga aggtgaagtg gaatcattaa atgctaccca
      721 attgcatgga ttataatcga accactatgc tagacgccga aacacctcaa gcacgccgcc
      781 gcatgcggct tctcgcaatc attattgcat ccataccctg ttattgtgcc gggttcgttt
      841 gtctttcctt tgctcccaac ccgcgcgatg tgaccccaac cccatccatc acagaaactg
      901 tgacgggcac cccgaacaca gccaccgcca gtagcacacc tttgatcgta accggcacaa
      961 ttacattcac gccaacaatc acttccacgc ctacatcaac atccacacct acggctacgc
     1021 tcacgttctt cctgcctagc accaacacat ttacatttac tcccaccaca accctcacct
     1081 tcacgcctac caatacattt acaccaacgg catcgaacac gccaaccatt acggcaacaa
     1141 tcactaatac gccaacccca acaaccacac tcactccatc ccctacccca taacttcatg
     1201 agcgatattc tccccttcct aaaatcgatt atctccatct ctggcgtttc tggggatgaa
     1261 gcccccgtag cggacctcgt tcaaaaaaag tggactccgc tggttgatga gatctcccaa
     1321 agtcgactcg gctcgattca tggactgaag cagggtacgc taaagaaaag cagttccaaa
     1381 gctggtagcg cttctcgccc atccgtgatg atcgccaccc acatggatgc catcggtatg
     1441 atggtcaccc gcattgtgga tggctttatt tatttcacca acgtcggcgg catcgactcg
     1501 cgtgtgctac cgggtacacc agtaattgtc cacgcttcgg ggacgaaaga agaactctac
     1561 ggcgtcatcg tcatgccgcc cggcaatttg ctccctgaag gcgaaggggg cggcgttgtg
     1621 cccatgaagt atctgttcgt tgatacaggt ttattcgcgg gcgaagtctc gaagcgagtc
     1681 cgtgtgggcg acagaatctc ttttggtaca gaaccagttg aaatgtccgg aggaaccgtg
     1741 agcggacaca cgctcgacaa tcgcgcctct gtggctgcgc tcaccgtctg ccttgaagaa
     1801 cttcaatcca aatcccatat ctgggactta tgggcagtcg ccaccgtgca agaagaaaca
     1861 tccttcggcg gttcttacag cagcaccttc caactgcgcc ccaccattgc cattgctctc
     1921 gatatgacct tcggcaaagg cgcaggcgca agcggatacc aaaccttccc gcttggcaaa
     1981 ggtgtgacct taggcatcgg acccagcgtt cacccgtttt tatatcagcg cttcaaagaa
     2041 gttgccgaac ggattgaaat tcccattgcc gatgacttga tgcccgaata ctcctccacc
     2101 gatgccgatg cgatgcagtt aattgctgaa ggcattccca ccatggtggt gagcattccg
     2161 caacgttata tgcacacagc cgtggagttg gtggcgttga aagatattca acgtgcgggt
     2221 cgtttgatgg ct
//
LOCUS       DAOQFT010000428         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32339_length_2224_cov_5.671277, whole genome shotgun sequence.
ACCESSION   DAOQFT010000428 DAOQFT010000000
VERSION     DAOQFT010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32339_length_2224_cov_5.671277"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLR93_10350"
     CDS             complement(<1..952)
                     /locus_tag="PLR93_10350"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27412.1"
                     /translation="MRVCVLSDEYIEDFNPAPYLKDYDWKMVTLTAPVEEKIKALAES
                     GEFDVFLNVCEGYEFEDEEDEELGYEASEVVEALEALNLPFTGADSRCFDPTREEMQT
                     IADANGVGFAKGYRVHSVEEAEQLVKNLRYPIMVKHPKSYGSTGMFKDSRADTLEQVL
                     TQVKRVCSEFGAARMEEFIVGKEFNVLVVDNPADMDNPFVYPPAELIFPDGEEFWHTD
                     IKWDYNIPFDFKEVTEPELKAKLQNEGKRMFQAMGISGYGRCDIRMNEKGELFILEIN
                     PNPAIMLPPKEYGPADYMILYDKDGYKGFFDRIFEAAFLRQ"
     gene            complement(1087..2124)
                     /locus_tag="PLR93_10355"
     CDS             complement(1087..2124)
                     /locus_tag="PLR93_10355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNH27413.1"
                     /translation="MTLFSEINEQPERIKSLLSSQRKHVEKIAAEIQKRGVDYVFLAA
                     RGTSDNAGRYANYLLGAMNGLPLALATPSLFTYYKKPPMLKNALVIGVSQSGKSPDIV
                     SVLEEGRRQGCMTLSITNEPNSPLAQKSDFVLDLQAGVEKAVAATKTYTTELMSIAML
                     SAALSGNKKAWDDLGNVSKWMRETLKQSDFIAQAVQRYRYIDQTVVLGRGFNYATAFE
                     WALKLKELTYIIAEPYSSADFAHGPIAMVESGYPVLAVAARGKVFDSMLEMLKRLRSD
                     IAAELVVISNDRKALLLAQVPLQIPVDTPEWLSPLVSILLAQLFAYHLTLAKGYNTEQ
                     PRSIRKVTETK"
BASE COUNT          607 a          594 c          445 g          578 t
ORIGIN      
        1 tctgccgaag aaatgctgcc tcaaagatac ggtcaaagaa gcctttatac ccatctttat
       61 catacaagat catataatct gcaggaccat attctttagg cggcagcata atagcagggt
      121 tggggttgat ctccaaaata aacaactcgc ctttttcgtt catacgaatg tcgcagcgtc
      181 cataaccaga aatacccatt gcctgaaaca ttcgttttcc ttcattttga agttttgcct
      241 ttagttcagg ctcggtcact tctttaaaat caaagggaat gttgtaatcc cacttaatat
      301 cggtatgcca aaactcttcc ccatctggga agatcaactc ggcaggcggg taaacgaagg
      361 gattgtccat atcagccggg ttgtcaacca ccaacacgtt gaactctttt ccaacaataa
      421 attcttccat gcgggcggca ccgaactcgc tacaaactcg ttttacctgt gtgagaactt
      481 gctcaagagt atcagctcgc gagtctttga acatgccggt actaccataa ctcttgggat
      541 gtttcaccat aataggatag cgcaaatttt tgacaagttg ttcagcttct tccacgctat
      601 gaacacgata gccctttgca aaaccgactc catttgcatc agcgatcgtt tgcatttctt
      661 cacgggttgg gtcaaagcag cgagagtctg cgccggtaaa aggtaaattg agcgcctcca
      721 gagcttctac aacttcactt gcttcatatc caagttcttc atcttcctcg tcttcaaatt
      781 cgtatccttc acagacattt aaaaagacat caaactctcc agattcagcc aacgctttga
      841 tcttctcttc cactggggct gtcaacgtga ccattttcca gtcgtaatct tttagataag
      901 gagcagggtt gaaatcttca atgtattcat cagacaaaac acagacacgc atagggtctc
      961 caaatttttg aaggatacgc aaataacagc tacacaaacg ccgctgtcta aatgcgtttg
     1021 cgattatcca cccatttccg attcagcaat tgatttttta agaaaaatag agcagattcc
     1081 tctgctctat ttggtttccg tcaccttgcg gatgctgcgt ggctgctctg tgttgtaccc
     1141 cttggcgagg gtcaaatgat aagcaaacaa ttgagcaagc aaaatgctca ccaatggaga
     1201 aagccattct ggcgtatcaa ccggaatctg caatggaact tgcgccaaca acagagcctt
     1261 ccggtcgttc gagatcacca ccaactcggc agcaatatca gaacgcaggc gtttgagcat
     1321 ctcaagcatc gaatcaaaga ccttacctct ggctgccact gccaaaacag ggtagccgct
     1381 ctccaccatg gcaatcggac catgcgcaaa atcggcagaa gaatatggct cggcaataat
     1441 ataggtcaac tcttttagtt tcaatgccca ttcaaatgcg gtcgcataat taaacccgcg
     1501 ccccaagaca accgtttgat caatataacg atagcgctgc acagcctggg caataaaatc
     1561 gctttgcttg agcgtttcac gcatccactt tgaaacgtta ccgagatcat cccacgcctt
     1621 tttatttccg ctcaatgccg ccgaaagcat cgcgatcgac atcaactcag tcgtataggt
     1681 ctttgtagct gcaactgctt tctctacccc ggcttgaaga tccaacacaa aatctgattt
     1741 ctgcgccaac ggcgaattcg gctcgttcgt gatcgaaagc gtcatgcagc cttgccgccg
     1801 cccttcctcc agcacactca caatatccgg cgacttaccc gattgcgaaa caccaatcac
     1861 caatgcattc ttcaacatcg gcggtttttt gtaataggtg aacaaagaag gtgtcgccaa
     1921 cgcaagcgga agtccattca tggcgccgag caaataattg gcataacgcc cggcattatc
     1981 cgatgttccg cgagcggcga gaaagacata atccacgcct ctcttttgga tttccgccgc
     2041 aattttttct acatgcttgc gctgcgaaga cagcaggctt ttgatacgct ccggctgttc
     2101 attgatttca gaaaataaag tcatgatgaa aatactccac gcctcattcc aagccgggaa
     2161 tggataccgg caaacgcccc gtcatctcgc tatgaccgaa tacggcttta gccaccgccc
     2221 gcat
//
LOCUS       DAOQFT010000429         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32510_length_2218_cov_4.780860, whole genome shotgun sequence.
ACCESSION   DAOQFT010000429 DAOQFT010000000
VERSION     DAOQFT010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32510_length_2218_cov_4.780860"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /gene="rimI"
                     /locus_tag="PLR93_10360"
     CDS             <1..343
                     /gene="rimI"
                     /locus_tag="PLR93_10360"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNH27414.1"
                     /translation="SSRCWVAVIDKQIVAMIVSWLLVDELHVATIATHPDFRERGIGK
                     RLLVHALLAAKAEGATSSILEVRASNQAALKMYHGFGYVEDGRRKEYYKDNNEDAILM
                     SLNGLDHLSGE"
     gene            345..989
                     /locus_tag="PLR93_10365"
     CDS             345..989
                     /locus_tag="PLR93_10365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNH27415.1"
                     /translation="MNAEETLAQLARDVATCTKCALHEARKKVVAGEGSANAEIMFIG
                     EGPGFHEDEQGRPFVGASGKFLDQLLAQADITREDVFIANVVKCRPPGNRDPKPEELA
                     MCDEYLEAQIRAINPSIIVTLGRISMGKYFPGAKITSVHGQMQNISGRFIIPMYHPAA
                     ALHQPALRPSILADFANLPNQLNEARQALGKKIVIQNKETSSLSIEKPKQLKLF"
     gene            1023..>2218
                     /locus_tag="PLR93_10370"
     CDS             1023..>2218
                     /locus_tag="PLR93_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNH27416.1"
                     /translation="MSRKDSLIKKLREKTAKVAILGLGYVGLPLAVVFGEAGFHVTGI
                     DPDKRKVDSLKKGLSYIPDVKTEAVNNLVKTGKLDATTDFSILKEMDAVSICVPTPLR
                     QTGDPDMSFIISAMEELSKYMHEEMVVVLESTTYPGTTRELLLPKLGTEHNLIVGEDW
                     FLAFSPERVDPGREDWTTINTPKVMGGITHECGEVATAWYEGAIKTVHHVSTAEAAEM
                     SKLLENTFRMINIGLVNELTIMCEFLGVDVWEVIDAAATKPFGFMKFTPGPGLGGHCI
                     PIDPLYLSWKMRSFNYNARFIELASEINTNMPRYVVSRVLEAMNDLGKTLKGSKVLVL
                     GAAYKPDIDDVRESPALDVIGLLRKKGALVEYHDPYIQHIHHEYDGWKMDSVNDVMKA
                     VEEADVV"
BASE COUNT          638 a          468 c          557 g          555 t
ORIGIN      
        1 atcctcccgc tgttgggtgg ctgtaataga caagcagatc gtggccatga tagtgagttg
       61 gctccttgtg gatgaattgc atgttgccac aattgccaca cacccggact ttcgtgaaag
      121 agggatcggc aaaagattgt tagttcatgc cctgttggcc gccaaagcag aaggcgccac
      181 aagctcgatc cttgaggtac gcgcaagcaa ccaggcggcg ttgaagatgt atcatggttt
      241 tggttatgtg gaagatgggc gccggaagga atattacaag gataataacg aggatgccat
      301 cctaatgtct ttaaatgggt tggatcacct ctctggagaa tgacatgaac gccgaggaaa
      361 cccttgccca acttgcccgg gatgttgcga cctgtacaaa atgcgccttg catgaggcac
      421 gcaagaaagt ggttgcaggc gagggctctg caaatgcgga gatcatgttc attggagaag
      481 ggccaggctt tcatgaagac gagcaggggc ggccattcgt aggggcatcg ggtaagtttt
      541 tggatcaatt attagcccag gcagatatca cacgcgaaga tgtattcatt gccaatgtgg
      601 tcaagtgccg cccgccaggg aatcgtgacc caaaacctga agaattggca atgtgcgatg
      661 aatacctgga ggcgcaaatt cgggcgataa atccgagtat cattgtgaca ttggggcgca
      721 tttcgatggg taaatatttt cccggcgcaa agatcacttc cgttcatgga caaatgcaaa
      781 atattagcgg aaggtttatc attcccatgt accatccagc cgcagcattg catcaaccag
      841 cattacggcc ctccatcctg gcagactttg caaacctgcc gaatcagttg aacgaagcac
      901 gccaggcatt aggtaaaaag attgttatac aaaacaaaga aacaagttct ctttccatag
      961 agaaaccaaa acaactaaaa ttattctgac tacataaaag ttattgaaag gaccaattaa
     1021 atatgtcaag aaaagattct ctaattaaga aattacgaga gaagacagct aaagttgcaa
     1081 ttcttggact tgggtatgtc ggcctgcccc tagctgtagt gttcggtgag gcgggttttc
     1141 atgtcacagg aatcgaccca gataagcgaa aggtggattc actaaaaaag ggattatcgt
     1201 atattcctga cgtaaaaacg gaggctgtta acaacctggt aaagactgga aagctcgatg
     1261 caactacaga tttttccatt ctgaaagaaa tggatgccgt tagcatttgt gtacccacac
     1321 cacttcgcca aactggtgac ccggatatgt cgttcattat ttcagccatg gaagaattaa
     1381 gcaaatatat gcacgaggag atggttgtgg tgcttgaatc taccacctac ccgggtacaa
     1441 cacgcgaact ccttctacca aagcttggaa cagaacacaa cttgattgta ggagaagatt
     1501 ggtttctggc cttttctcct gagcgtgttg acccaggccg cgaggattgg actaccatta
     1561 acacacccaa agttatgggc ggtatcacgc atgagtgcgg ggaggttgca accgcatggt
     1621 atgaaggcgc gattaagact gtacatcatg tctccactgc agaagctgct gaaatgtcaa
     1681 aacttcttga aaatactttc cgcatgatca acatcggttt ggtcaatgaa ctgaccatca
     1741 tgtgcgaatt tttaggggta gatgtttggg aagtgatcga tgctgcagcg accaagcctt
     1801 ttggtttcat gaagttcaca ccaggccctg gcttgggcgg gcactgtatt ccgattgatc
     1861 cactttacct ttcatggaaa atgcgttcat tcaactacaa tgcccgattt atcgaattgg
     1921 cctctgagat caacacaaac atgccgcgct atgttgtcag tcgtgtactt gaagcaatga
     1981 acgatcttgg caagactttg aaaggttcaa aagttttagt attaggtgca gcctataaac
     2041 ctgacatcga tgatgtgcgt gagtcgcctg cattggatgt gatcggctta ttacgcaaaa
     2101 aaggtgcatt ggtcgaatac catgacccat atattcagca tattcatcat gaatatgacg
     2161 gctggaaaat ggatagcgtg aatgatgtga tgaaggctgt agaagaagct gatgtagt
//
LOCUS       DAOQFT010000430         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32513_length_2218_cov_3.740176, whole genome shotgun sequence.
ACCESSION   DAOQFT010000430 DAOQFT010000000
VERSION     DAOQFT010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32513_length_2218_cov_3.740176"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLR93_10375"
     CDS             <1..338
                     /locus_tag="PLR93_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNH27417.1"
                     /translation="EDAFPFGRDESEVRLGRAGRYAFGFYEKHPCYAPWSHTLIDFNG
                     NVYVCCMTREKIPPLGNIIHQSFKEIWEGAAYRRVRLKMHPPSLKPCARCDDFIDENK
                     KIWEMIGPY"
     gene            complement(335..952)
                     /locus_tag="PLR93_10380"
     CDS             complement(335..952)
                     /locus_tag="PLR93_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27418.1"
                     /translation="MNVLLLHGQGRTTNAMRLLGFRLHRLGYHVCYFRYYTRIEKFSG
                     IVERLTDTIKKLPNDQPYALVGHSLGGLLARACLPSLKDHPPFHLILLASPNRPPQLA
                     PYIKHNRIYKYLTNDCGQQALNAEFYNTLPKPFVPTTIIAGSGGPRLRWLPYGDQPND
                     GVMSVQDTRLGPEYGVIQVPSIHTFIMNSRQTLSHIDRILRQTGI"
     gene            complement(959..1237)
                     /locus_tag="PLR93_10385"
     CDS             complement(959..1237)
                     /locus_tag="PLR93_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27419.1"
                     /translation="MAKCEMVIGYLVPHRCDNPALGTCVKCGRGFCDEHTNATNEGRV
                     CLACQQGLDMPVALPIAASTFTAADLATFGHASTWDDDDSNDMFSDLS"
     gene            1249..>2218
                     /locus_tag="PLR93_10390"
     CDS             1249..>2218
                     /locus_tag="PLR93_10390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH27420.1"
                     /translation="MKIALLSEKYTPDLGGLAISTGRLGEMLASAGHDVRLFAPTTSL
                     PPTIKQTQRSSGVHVTRFGAHKRVDDTLVDWFELIVEEHKREPFDLIHAYFLPMAGFV
                     GTYAGKYLGIPSVVSIRGNDIERAAFDPGKFSHVMYALQNANAVTTNATVLAKKAIAF
                     VEREIHVIPNGIDTDCFKPMERNEVLAEAIGLSMESGGLPPEIGKQAPRLHKYVIGFV
                     GELREKKGMVTLLYGYAQLAKKMPVSLLIVGEVREGEDKKAFDEFKISNPQLPITVTG
                     HIPHKDLPAYYSLMDIFVHPSLRDGMPNAVLEAMACGKTVIATPVGG"
BASE COUNT          585 a          560 c          552 g          521 t
ORIGIN      
        1 atgaagatgc ttttcccttt gggcgagacg agtccgaggt ccgcttggga cgcgcgggga
       61 gatacgcctt cggcttctat gaaaagcatc cctgctatgc tccctggtcg catacattga
      121 tcgacttcaa tggcaatgtc tatgtctgtt gcatgacgcg tgaaaaaatc ccacccttgg
      181 gaaacatcat ccaccaatca ttcaaggaaa tttgggaagg tgcggcctat cgtcgggtac
      241 gattgaaaat gcatccgcct tccctcaagc catgcgcacg ttgtgacgat tttattgatg
      301 agaataaaaa gatctgggaa atgatcggac cgtattaaat ccccgtctgc cttagaatgc
      361 gatcgatatg ggaaagggtc tgacgtgaat tcatgataaa cgtatgaata cttggaacct
      421 ggatcacgcc atattccggt ccaagacgag tatcctgaac gctcatgact ccgtcgtttg
      481 gctggtcacc ataaggcagc caacgcagcc gagggccgcc agagccagca atgatcgtcg
      541 tcggaacgaa tggctttggc agcgtgttat aaaactctgc attcaacgcc tgttgtccac
      601 agtcattggt cagatattta tagatacggt tatgttttat ataaggtgcc aattgaggag
      661 gccggtttgg agaagccagt aaaatcaaat gaaatggcgg atgatccttc aatgaaggaa
      721 ggcaggctcg tgccagcaac ccacccaggg aatgccccac caaggcatat ggttgatcgt
      781 tcggtagttt tttgatcgta tcggtcaacc gctccacgat cccgctgaat ttttcaatgc
      841 gggtgtaata tcggaaatag cacacatgat acccgagcct gtgcaaacgg aaaccaagca
      901 gcctcatggc attcgttgtt cgcccctgac catgaagcaa aagaacgttc atggattttc
      961 aactcaaatc cgaaaacata tcgttgctgt catcatcgtc ccacgtactg gcatgaccaa
     1021 aagtagccag atcggcagca gtaaaggttg aagcagcgat cggcaaagca accggcatat
     1081 ccaaaccctg ctgacaggca agacaaactc gtccttcatt ggttgcattc gtatgttcat
     1141 cacaaaaccc gcgcccgcat ttgacgcagg tccccaacgc cgggttatca caacgatgcg
     1201 gaaccagata accgataacc atttcacact ttgccattga gacacctcat gaaaattgct
     1261 ttgcttagcg aaaagtatac accagacctt ggagggctcg caatctctac aggacgattg
     1321 ggggagatgc ttgcctcggc tggacatgac gtccgcctct ttgccccgac tacaagcctg
     1381 ccacccacca tcaaacagac tcaacgctca agcggagtcc acgtaacgcg ctttggcgcg
     1441 cacaaacgcg tggatgatac cctggtcgat tggttcgagc tgatcgtgga ggaacacaag
     1501 cgtgagccgt tcgatctcat ccatgcctac tttttgccaa tggcgggatt cgtcggtacg
     1561 tatgctggga agtatctcgg cattcccagc gttgtctcca ttcgcggaaa tgacatcgaa
     1621 cgcgccgcat tcgaccctgg caaattttcg catgtgatgt atgccttgca aaacgccaat
     1681 gctgtgacaa ccaatgccac agtattagcg aaaaaagcaa tagcattcgt tgaacgtgaa
     1741 attcacgtca tccccaatgg aatcgacacc gattgcttca agccgatgga gaggaatgag
     1801 gtactggcag aggcgattgg gctatctatg gagtctggcg gcttaccgcc ggaaatcggg
     1861 aagcaagctc cccgactcca taaatatgta attggctttg tgggtgaatt gcgtgaaaag
     1921 aagggaatgg ttactctttt atatggctac gcgcagttgg cgaagaaaat gcccgtttcc
     1981 ctgctcattg tcggagaagt ccgcgaaggc gaagataaaa aagcatttga cgagttcaaa
     2041 atctccaatc cccaattacc aattacagtt accgggcata tcccccacaa agacctgcct
     2101 gcctattatt ccttgatgga tatcttcgtg catccgtctt tgagagatgg catgccgaat
     2161 gcggtgctcg aagcgatggc gtgcggaaag acggtcattg cgactcccgt tggcggtg
//
LOCUS       DAOQFT010000431         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32532_length_2217_cov_6.884366, whole genome shotgun sequence.
ACCESSION   DAOQFT010000431 DAOQFT010000000
VERSION     DAOQFT010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32532_length_2217_cov_6.884366"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLR93_10395"
     CDS             <1..1018
                     /locus_tag="PLR93_10395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNH27421.1"
                     /translation="ARALGIRSAMPMSRAVRLCRDLIIVPGRHHLYGEYSAKVMEKLH
                     NLTPLVEQISIDEAFLDISDIQADKTRLALDLQLSIRNELNLPSSIGIASNKLVAKIA
                     TEVGKKSSKKKNEPPFGFTVVPVGDEAKFLDPLPADMLWGVGPKTNARLAELGIHTIG
                     DIARWPEKELVSLFGENGRDLWQHAHGMDNRPVSTESETKSISQETTFNVDISDQKTL
                     EKTLREQAHEVARQLRKNDLAGKTVKIKIRWPDFTTLTRQTTLPTSTDNEDDIYRAAV
                     KLMDSVRKPNQAVRLLGVGVSGIGAPVRQLSLWDVGSEKSRKLQEVVDELQEKYGKDV
                     IWKG"
     gene            1096..2058
                     /locus_tag="PLR93_10400"
     CDS             1096..2058
                     /locus_tag="PLR93_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNH27422.1"
                     /translation="MTNTATRLITLIFLLQNQPNQKASELAEKLGVSLRTVHRYFEML
                     DEMGVPVYSERGPYGGFSLVRGYKMPPLVFTLEEAVAVVLGTGLVQELWGDLYRESAN
                     GALAKLENLLPEEQTREVAWARSSLAVTGLHRANLKMQTPALEKLRRAIRELRSVNML
                     YQTSQVPHPAERGLDPYALVHRWGWWYVIGLCHVHNEVRTFRVDRISEVTLSENIFVK
                     STGFDLQAYLNMEMQSQPQITARLKFDVASANIVSGNRSYWQNVEPQADGSVEVTFLS
                     PTLEWAASTVLMYGPAVEVLEPPALRSMVAEWLEVTIRKYTEKE"
BASE COUNT          622 a          544 c          544 g          507 t
ORIGIN      
        1 ggctcgcgcg cttgggattc ggagtgccat gcccatgtcg cgagcggtgc gcctgtgtcg
       61 tgatttgatc atcgtacccg ggcggcacca tttgtatgga gaatactctg ccaaggtgat
      121 ggaaaagctg cataacctga ctccactcgt ggagcaaatt tccatcgacg aagccttcct
      181 cgacatctcg gatattcaag cggataaaac ccgcctcgcg ctcgacctgc aactgtccat
      241 acgaaatgag ctgaatctac cctcgagcat tgggatcgcg tccaacaaac tcgtggcaaa
      301 aatagccact gaggttggta agaaatcaag caagaaaaag aatgagccac cattcggctt
      361 caccgttgtt cctgttggag atgaagccaa atttctagac ccactccccg ctgatatgct
      421 ttggggcgtg ggaccaaaaa ccaatgcaag gttagccgag cttggcattc ataccattgg
      481 ggatattgcc cgctggcccg aaaaggaact cgttagcctc ttcggcgaaa atggacgtga
      541 cctctggcaa catgcacacg gcatggataa tcgtccggtc agtaccgaat cagagaccaa
      601 gtctattagc caggaaacca cgttcaacgt agatatcagc gaccaaaaga ccttagaaaa
      661 aacattgcgc gaacaagctc acgaagtggc tcggcaactt cgcaaaaatg acctcgccgg
      721 gaaaacagtc aaaataaaaa tccgctggcc cgacttcacc accctaacaa gacaaaccac
      781 cttacccact tccacagaca acgaagacga tatctaccgc gccgccgtca agttgatgga
      841 ctctgtccgc aagccaaatc aagccgtgcg gttgttagga gtcggcgtca gcggaatcgg
      901 agctccggtt cgtcaactca gcttatggga cgtaggaagc gaaaagtccc gcaagttaca
      961 agaagtagtg gatgaactcc aagagaagta tggcaaagac gtcatatgga aaggctagtg
     1021 ctcaaaaaaa attgctaacg ggtatatgtc ataatttgac atatacccgt tttatattat
     1081 tgaaggatgt aacacatgac caacactgcc acccgcctca ttacgctcat ttttctatta
     1141 caaaaccagc caaaccaaaa agcttcggaa ttggctgaga aacttggcgt ctcattgcgg
     1201 acagttcacc gctattttga gatgctggat gaaatgggtg ttccggtcta ttctgaacgc
     1261 ggaccgtatg gtggcttctc gctcgtgcgt ggatacaaaa tgccgccact ggtctttacg
     1321 ttggaagaag ccgttgctgt agtattgggc acaggcttgg tgcaagaatt gtggggagac
     1381 ttatatcgcg agtctgcaaa tggagctttg gcgaaactgg aaaatctttt gccagaagaa
     1441 cagacgcgtg aagttgcctg ggctcgcagt tcacttgccg ttacaggatt gcaccgtgcg
     1501 aatctaaaaa tgcaaacacc tgcgctagaa aaattacgcc gtgccattcg cgagcttcgc
     1561 agtgtgaata tgctctatca gaccagtcag gttccgcacc ctgcagaacg cgggcttgac
     1621 ccgtatgcac ttgtccatcg ttggggctgg tggtatgtaa tcgggctctg ccatgttcac
     1681 aatgaagtcc gcacattccg cgtggaccgt atttccgaag ttacgctgtc agaaaatatc
     1741 tttgtcaaat caacaggttt tgatttgcaa gcatatttaa acatggaaat gcaatctcag
     1801 ccgcagatca cagcgcgttt gaaatttgat gtagcttcag cgaacatcgt ttcagggaat
     1861 cgatcctatt ggcagaatgt tgaaccgcaa gcagatggct ctgtagaagt tactttccta
     1921 tctcccacgt tggaatgggc agccagcacc gtgttaatgt atggtcccgc cgtggaggtg
     1981 ctggaaccgc cagcgttgag gtcgatggtg gctgaatggc ttgaagtcac catccgaaaa
     2041 tataccgaaa aggaatgaag actatgaaaa tcttagatct aaaaaaacaa tataaaaatc
     2101 tatatcaacc ctcggcaaaa aggatcgaat tggtacaagt gccgaacctg caatttgcca
     2161 tggtcgacgg tgccatcgaa aaaggctccg aaccgggcaa atcgccatca tttgctc
//
LOCUS       DAOQFT010000432         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32533_length_2217_cov_6.635060, whole genome shotgun sequence.
ACCESSION   DAOQFT010000432 DAOQFT010000000
VERSION     DAOQFT010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32533_length_2217_cov_6.635060"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLR93_10405"
     CDS             <1..224
                     /locus_tag="PLR93_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNH27423.1"
                     /translation="GGTLIAINNAVFLSGKEYMQTLEKLCKDGYLSLRELIPVPDDFM
                     GTKRIQTPITDPAPFNHSTKIAILDVRRK"
     gene            221..616
                     /locus_tag="PLR93_10410"
     CDS             221..616
                     /locus_tag="PLR93_10410"
                     /inference="COORDINATES: protein motif:HMM:NF021514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNH27424.1"
                     /translation="MNGETELGKLLKNLKPERQPGEYVFCVVNTLKHAAELNPISTFQ
                     EAEGVTVVLPKRLADENILFYSVVCAWITLTVHSSLEAVGLTAAVSSALAGANISCNV
                     VAAFHHDHIFIPVKDAEQAMEILLSLSKG"
     gene            618..992
                     /locus_tag="PLR93_10415"
     CDS             618..992
                     /locus_tag="PLR93_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH27425.1"
                     /translation="MNPKQVLETFWEKMESNDFYAVAELLHDEFTLEWHQSGELIRGR
                     ENFAKINTAYPANGEWRFNINSIVAEGDVVVTDVSVTDGVVKDRVITFSTVRDGKIWK
                     QIEFWPEPFAAPEWRARWVEKI"
     gene            complement(989..2014)
                     /locus_tag="PLR93_10420"
     CDS             complement(989..2014)
                     /locus_tag="PLR93_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH27426.1"
                     /translation="MKLKTTLFLALFFFAFLIFSSPAKAQEFGPTPTLGAPPNPADII
                     NAVNNLRLSYGLPALYAHPVLMQIAQEQASGIAAGMPGHWRPNNMTLGQWMLSLGYPL
                     SGDLTLDGYRSENWGPASTAEDAIQMWLGDDLHTNTMLSTERSDIGAGVAIADGQFIV
                     VIETALQTRSGQMQSNAVSILTGIPQTQIAYSNMETQAAIDGVLPQYSIPVVLNTPFP
                     NGKVYHEVLYGQTLWSIAVAYGTTIKQIQGLNNLGDSTTVYEGQTLLVRTDATQPAPV
                     VTVTTENAVIPAEQTSTPAQTLLPLMETPQNSQNQRGPTFGVTAIILSALFLAGVFAV
                     MARKKEI"
BASE COUNT          623 a          487 c          520 g          587 t
ORIGIN      
        1 atggcggcac tctcattgcc ataaacaatg cagtttttct aagcggcaaa gaatacatgc
       61 aaaccctcga aaagctttgt aaagacggct atcttagtct tcgtgaattg atccccgttc
      121 cagatgattt tatgggaacc aaacgtatcc aaactcctat cactgaccct gcgcctttta
      181 atcattcaac taaaattgcg atcttagacg tgaggcgaaa atgaatggtg aaacagaact
      241 cggaaaatta ttgaaaaact tgaaaccaga acgccagcct ggcgaatatg ttttctgtgt
      301 tgtaaacaca ttaaagcatg cggctgaact aaaccccatc tctacatttc aagaagctga
      361 aggtgtgacc gttgttttgc ctaaacgact agcagatgaa aatattcttt tctattcagt
      421 agtatgtgca tggatcacgc tcaccgtcca ctcttctctc gaagcggtag ggctcacagc
      481 ggcggtcagc agtgcgttag caggagccaa tatcagttgt aatgtggtgg cggcttttca
      541 tcatgaccac atatttatcc cggtcaaaga tgcagaacag gcgatggaaa ttctgttatc
      601 cctatcaaaa ggctaagatg aatcctaaac aggtcttaga aacattttgg gaaaagatgg
      661 aaagcaacga tttttatgca gttgctgaac tcttgcatga cgaattcacg cttgaatggc
      721 atcaatcagg tgaattgatc cgcggcaggg aaaactttgc aaagatcaat acggcatacc
      781 cagccaatgg agagtggcgc ttcaatatca attccatcgt tgctgaaggg gatgttgttg
      841 taacagatgt atcagtcacg gatggggtgg taaaagatag agtcatcact ttttcaacag
      901 ttcgcgatgg aaagatctgg aaacagatcg aattctggcc cgaaccattc gcagcaccag
      961 aatggcgagc ccgatgggtg gaaaaaatct agatctcttt tttacgcgcc attacagcaa
     1021 acaccccagc aagaaataaa gcagaaagaa taatcgcagt cacaccaaac gtgggtccac
     1081 gctgattctg agagttttgt ggtgtctcca ttaaaggcag gagcgtttgt gcaggtgttg
     1141 atgtctgttc ggcgggaatc actgcgttct cagttgttac ggttacaaca ggtgcaggtt
     1201 gggtggcatc tgttcggaca agcagtgttt gcccttcata aacagttgtt gagtctccta
     1261 gattatttag tccctgaatt tgcttaatcg tcgttccgta agctacagca atgctccata
     1321 aggtttgtcc atacaatact tcgtgataca ccttgccatt cgggaaggga gtattcaata
     1381 ccacgggaat ggaatattgc ggcaatacgc catcgatcgc agcttgtgtc tccatgttgg
     1441 aatatgctat ttgcgtttga ggtattcctg taaggatcga aaccgcgttc gattgcattt
     1501 gtccactgcg ggtttggagt gctgtttcta tcaccacgat aaattgtcca tctgcaatgg
     1561 cgacacccgc tccaatatca cttcgctctg ttgagagcat ggtatttgtg tgcaggtcat
     1621 ctcccaacca catttgaatt gcatcctctg cggttgatgc gggtccccag ttttcggagc
     1681 gatagccatc caaggtcagg tcaccagaca gtggatagcc caatgagagc atccactgtc
     1741 ccaacgtcat gttattaggt cgccagtgac cgggcatacc agcagcaatt ccgctggctt
     1801 gttcttgggc aatctgcatt aatacggggt gggcatacaa tgctggcaac ccatatgaaa
     1861 gccgcaaatt attaaccgca ttgataatat ccgccggatt gggtggagca cctaatgttg
     1921 gagtaggtcc aaactcctgc gctttggctg gagaagagaa gattaaaaac gcaaaaaaga
     1981 ataaagcaag aaatagtgtg gtttttagtt tcatcccata catcatgttg cacatcctac
     2041 ctgaatcata acaggcaaaa taaaagccga agatttggtt cttcggcttt tgaaagttta
     2101 tggtctccaa gagggcgcat accagtcata gtctgtagaa agccccggag cttgcaaaat
     2161 gctataactc gaagtagcta ggtctaccag atacaagtgt ccgccagaag ccgatgt
//
LOCUS       DAOQFT010000433         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32560_length_2216_cov_4.892180, whole genome shotgun sequence.
ACCESSION   DAOQFT010000433 DAOQFT010000000
VERSION     DAOQFT010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32560_length_2216_cov_4.892180"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLR93_10425"
     CDS             complement(<1..220)
                     /locus_tag="PLR93_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH27427.1"
                     /translation="MQNDLLLKRLKTFEFLRGLDDETLSMLAKSAVWKVFPPNAVVFW
                     EGNVETNLFYLEYGSLKAMRTSPDGREQV"
     gene            complement(253..1101)
                     /locus_tag="PLR93_10430"
     CDS             complement(253..1101)
                     /locus_tag="PLR93_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNH27428.1"
                     /translation="MDSLSERRKAIFYLAITAILWSSSGLFVKILDWHPVAILAGRSF
                     FAAIVFLIYLRRIPTRFGLWQLLAAAMFILTQFLFITSTKMTTAANSIFLQYTMPIYV
                     VLLAYWFLGEKPSRTDWIAMLVILIGLALFFGDELSTEGFYGNLLAILSGVTGAIMMV
                     SFRAQKDGNPAESNLIAFLVTATFGFPFIMKETWTVNSILILAFLGIFQIGVAFIFFS
                     KGIKHIPALEANLIGTLEPVLNPIWVFLFYGESMGKFALIGGLIVLSGVILSAVGSAR
                     AAREGH"
     gene            1222..2163
                     /locus_tag="PLR93_10435"
     CDS             1222..2163
                     /locus_tag="PLR93_10435"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNH27429.1"
                     /translation="MKTGIVGSGFVGATAAYALVMRGVGRRVVLVDMNKARALAEADD
                     ILHAVPFAHQLEVTAGDYEDLADCKVVVVSAGVGQKPGETRLELLGRNAKVFEQVIPN
                     ILTHAPNAILLIATNPVDVMTHIAAHYAAKMGVPSNRVIGTGTTLDTARMRTLLGRHL
                     KVDSQHIHAYVLGEHGDSELLPWSLVTVGGIPLDEFCSDDDISICPDDYEDIDMRVRR
                     AAYRIIDGKGATYYGIGSAIARIVEVIQQDERSILTVCTPMPDVAGVKDVTVSLPNLI
                     GGEGIIQTFFPNLDPNEMDALRHSASVVRSAIDQLKF"
BASE COUNT          577 a          531 c          577 g          531 t
ORIGIN      
        1 acacctgttc gcgtccatcg ggtgaagtcc gcatggcttt gagtgaacca tattccagat
       61 agaacagatt cgtttccaca ttcccttccc aaaacaccac cgcatttggc ggaaagacct
      121 tccatactgc gctctttgcc agcatagaca gagtctcatc atcgagtccg cgcaggaatt
      181 caaaagtttt aagtcgcttt aggagcaggt cattttgcat agcatatgga ttgtatcgta
      241 cgaagtagaa gcctagtgtc cttctctggc tgctcttgca cttccaaccg cgctcaaaat
      301 cacaccgctt aaaacgatca acccgccgat gagcgcaaac ttgcccatgc tctcgccata
      361 gaataaaaat acccaaatgg gattaagaac cggctcaagc gtaccgatga gattcgcctc
      421 caatgccggg atgtgcttga tgcccttgga gaaaaagata aacgccactc cgatctgaaa
      481 aatgccgagg aaagcgagga tcagaatgct gttcaccgtc cacgtttctt tcatgatgaa
      541 gggaaaacca aatgttgcgg tgacaagaaa ggcaatgaga ttgctctctg ctgggttgcc
      601 atctttttgc gcgcggaagg aaaccatcat gatcgcgccg gtcacaccgc tgaggatggc
      661 aagtaaattg ccatagaacc cttctgtgct gagttcatct ccgaaaaata atgccagccc
      721 aatcaagatc accagcatcg cgatccaatc tgtgcgtgaa ggtttttcac cgaggaacca
      781 ataggctaac aaaaccacat aaatcggcat ggtgtattgc aaaaagatcg agttcgccgc
      841 cgtggtcatc ttggtagagg tgatgaacag gaactgggtc aggatgaaca tggcagctgc
      901 cagtaactgc cacagcccaa agcgagtcgg gatgcgccgc aaatagatga ggaaaacgat
      961 cgcggcgaag aagctgcgtc cggcaaggat ggcgaccgga tgccaatcca atattttgac
     1021 aaaaagtccg cttgagctcc aaagaatagc ggtgatagcg agataaaaaa tcgccttacg
     1081 gcgttctgaa agagagtcca ctgagttcct cacaaggggt agattggtgc cgattatacg
     1141 cgttaacttg ggaggagttc tagtaaaatc atttcgttat tggttctgag ctattaaatt
     1201 taacgataga ggagtttaac aatgaaaaca ggaattgtag gcagcggctt tgtaggcgcc
     1261 actgctgcgt atgccttggt gatgcgtgga gtggggcggc gcgtggtttt ggtggatatg
     1321 aacaaagcgc gtgccttggc agaggcagat gatattttgc atgctgtgcc atttgcacat
     1381 cagttagagg tgactgccgg cgattatgaa gaccttgctg attgcaaagt ggtggtggtt
     1441 tctgccgggg tggggcaaaa gccaggcgag acccgtttgg agttattggg gcgaaatgca
     1501 aaagtgtttg aacaagttat tcccaatatt ttgacacatg cccccaatgc gattttgttg
     1561 atcgcgacca accctgtgga tgtgatgacg cacatcgcgg ctcattatgc cgccaagatg
     1621 ggtgtgccat ctaaccgcgt gatcggcacc ggcaccactc tagacacggc tcgtatgcgc
     1681 accttgctgg gacgtcacct caaagtggac tctcaacaca tccatgctta tgtgcttggc
     1741 gagcatggcg actcggaatt gctgccatgg tcgcttgtca ccgtgggtgg aattccgctc
     1801 gatgaattct gttcagatga cgatatctcg atctgccccg atgattatga agatattgat
     1861 atgagggtcc gacgagcggc gtatcgcatc atcgacggca aaggcgcgac ctattacggt
     1921 attggcagcg ccattgcccg cattgtggaa gtgattcaac aagatgagcg atccattctc
     1981 acggtttgta cgcccatgcc cgatgtggcg ggcgtcaaag atgtgaccgt ctccttaccc
     2041 aacttgatcg gcggtgaggg catcatccaa acgttcttcc ccaatttaga cccgaacgaa
     2101 atggatgcac tccggcatag cgccagcgtg gtccgctccg cgatagatca attgaagttc
     2161 taatcttgaa acaaaagagc gtgataccaa tcacgctctt ttgttttttt ttatgc
//
LOCUS       DAOQFT010000434         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32589_length_2215_cov_7.296296, whole genome shotgun sequence.
ACCESSION   DAOQFT010000434 DAOQFT010000000
VERSION     DAOQFT010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32589_length_2215_cov_7.296296"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLR93_10440"
     CDS             complement(<1..244)
                     /locus_tag="PLR93_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27430.1"
                     /translation="MNKDILGSFFNRILPLFPPRTEAVLKGMTTSTLEDMNFRYIFTV
                     DGMVTISHIIEEMINKNKGVADLHVSFQYFSRFADQE"
     gene            complement(263..1540)
                     /locus_tag="PLR93_10445"
     CDS             complement(263..1540)
                     /locus_tag="PLR93_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27431.1"
                     /translation="MNKRLLSIILVVFIDLLGFSLILPLLPYYAEKYGATQFVTGLLV
                     ASYAAMQLIGAPLLGRLSDRYGRRPILLASVFGTFLGFLLLGFADNIGTAMANAFDPQ
                     AANIFVLAILFVSRMVDGLTGGNLSVAQAYISDVTDAENRSKGLGMIGAAFGLGFIIG
                     PATGGILSQYGYAIPGFVAAALSFVNLSLIYFWLPESLTEEKRAEMTDKKPAVTLSAL
                     TKALTRPFTGALLTTRFFYGLAFSIFQTVFALYALQKFNFEARDTGFTLTFVGILSVF
                     VQGFLIGRLTKKFRDDNLILLGGVLMTISLLGWALTPNVFWLLIVLVPTAISGGILNT
                     LLSSTLTKAVQPQEIGGILGLGAAIESATRIFAPIIGGYLLQTFGTWSPGAFGAVMML
                     GITIFIYFTIYNHPIASEIRAKSIQQAPAPNGD"
     gene            complement(1623..>2215)
                     /locus_tag="PLR93_10450"
     CDS             complement(1623..>2215)
                     /locus_tag="PLR93_10450"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNH27432.1"
                     /translation="VLVTLWGLRLSLYIFSRNHGQPEDFRYAKWREEAGSAWWWRSFF
                     KVFLLQGVIMWVIAIPLIAAQTSTPSPFKCECTDYTGAALWLVGFIFEAGGDWQLARF
                     KKDPANKGKLLTTGLWSLTRHPNYFGDAAQWWGFWLLAFANGAWWTFFSPLIMTYLLM
                     RVSGVTLLENTLTKTKPGYEEYMARTNAFFPWFPKK"
BASE COUNT          608 a          495 c          602 g          510 t
ORIGIN      
        1 cttcttgatc ggcaaagcgc gaaaaatatt gaaaagagac atgcaggtca gctacacctt
       61 tgttcttgtt gatcatttcc tcaatgatgt ggctgatggt caccatgcca tcgacggtga
      121 agatataacg aaagttcata tcttccaacg tcgatgttgt catgcctttc aagaccgctt
      181 ctgtgcgcgg agggaagagc ggtaggatac ggttaaagaa actcccaaga atatctttat
      241 tcattaaaaa gtcctttaag aattaatcac cgtttggcgc aggcgcttgc tgtatcgatt
      301 ttgcgcggat ctcacttgca atggggtggt tgtagatggt gaagtaaata aagatggtaa
      361 tgcccaacat cattacagcc ccgaaagctc ctggagacca ggtgccaaaa gtttgcagta
      421 aatatccgcc gatgatcggt gcaaagatac gtgtggcact ttcaatggca gcgcccaacc
      481 cgagaatgcc gccgatctct tgcggttgaa cggctttcgt aagagtggag gataggagtg
      541 tattgaggat gccgcccgaa atagcggtgg ggactagaac tattaagagc caaaaaacgt
      601 tgggtgttaa tgcccagcca agtaatgaga ttgtcatcaa aaccccgccc aaaagaatta
      661 ggttgtcatc acggaatttc ttggtcagtc tgccaatgag gaatccttga acaaaaacag
      721 acaaaatgcc tacgaacgtg agtgtaaaac cggtatctct tgcttcgaag ttgaattttt
      781 gtaaagcata taacgcgaac acggtttgga aaatggaaaa cgccagccca taaaagaagc
      841 gcgttgttag aagtgcgccg gtgaatggac gggtcagcgc cttggtcaat gcagaaagcg
      901 taaccgctgg ttttttatct gtcatttcgg cacgtttttc ttcggtcaag gattcaggta
      961 gccagaaata gatcaacgag aggttgacga aagacaacgc cgccgctaca aagccgggaa
     1021 ttgcgtagcc atattggctt aagatgccgc cggtagcggg tccgataatg aagcccaatc
     1081 cgaatgccgc accgatcatg cccaaacctt tcgaacggtt ctcagcatcg gtcacatcag
     1141 agatataggc ttgtgccacg ctcaagttcc cgccggtgag tccatctacc atgcggctga
     1201 cgaagagaat cgccaatacg aagatgttag cagcttgagg gtcgaaggca tttgccattg
     1261 cggtgccgat attgtctgca aagccaagca ggaggaagcc gaggaaggtt ccaaagacag
     1321 atgccaacag aatggggcga cgtccgtaac ggtctgagag gcgacccaat aagggtgcgc
     1381 cgatcaactg catggcggcg tatgaagcga cgagtaagcc ggtcacgaat tgggtcgcgc
     1441 cgtatttttc cgcgtagtag ggcaaaagcg gaaggataag actgaagccg agcaagtcaa
     1501 tgaaaacgac cagaatgatg gagagtaagc gtttgttcat agtttccttt tggttcaagt
     1561 tgcacgaagc cacacaaagg tcgactttgc gttatggctt tcgttttgaa agggtggata
     1621 cgttattttt tagggaacca cgggaagaat gcattggtgc gtgccatgta ttcttcataa
     1681 ccgggtttgg ttttggtcag cgtgttttcg agcaatgtca cgccagaaac tctcattaag
     1741 agataggtca ttattaaggg actgaagaag gtccaccagg ctccgttggc aaaggcgagg
     1801 agccagaagc cccaccactg tgccgcgtca ccaaagtaat tggggtgacg ggtgagactc
     1861 cacaagccag tggtgagcag tttgcctttg tttgcggggt ctttcttgaa gcgtgccagt
     1921 tgccaatctc cgcctgcttc gaagatgaag ccgaccaacc ataatgcagc gccggtatag
     1981 tcagtgcatt cgcatttgaa cggcgaaggc gtggatgtct gcgcagcgat caatgggatg
     2041 gcgatgaccc acatgatgac gccttgcaga agaaaaactt tgaagaaact gcgccaccac
     2101 caagctgacc cagcttcttc tcgccatttg gcatagcgga agtcttcagg ttgaccgtgg
     2161 ttgcgcgaaa agatgtaaag ggataggcgc agcccccaaa gagtgaccaa cacac
//
LOCUS       DAOQFT010000435         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_32842_length_2206_cov_6.449093, whole genome shotgun sequence.
ACCESSION   DAOQFT010000435 DAOQFT010000000
VERSION     DAOQFT010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32842_length_2206_cov_6.449093"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLR93_10455"
     CDS             complement(<1..432)
                     /locus_tag="PLR93_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNH27433.1"
                     /translation="MLRSFFIYLSKAAWAQKLITGWSFSWRIASRFVAGVTLTDAIKI
                     VHELNAKGINATLDHLGEHTNTPEEAQQAADSIFATLDAIGADPSLRSNISIKLTQIG
                     MGLDESLCAETLERILARAKTAKTFVRVDMEDTPYTDKTINL"
     gene            558..2048
                     /locus_tag="PLR93_10460"
     CDS             558..2048
                     /locus_tag="PLR93_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNH27434.1"
                     /translation="MRNRNTNTILRGVSILFLLGAFTLTVVSLISYSRQRTGYPAGMM
                     IAGVPVDGLSPAEASQRLLQVYNSPIEVNYNGAFFHIDPGAVGFTMDVETMLAAADLT
                     RTGGSFWGEFWDYLWNRAPAESEIPLSASISEDRLREFLQNEIASRYDIPPAPAQPVP
                     GGTTFLPGAPGQTLDVNRAVVIITNALRSPADRSVVLTFNQTTASRPTIENLEILLKQ
                     IITTSDFDGLIGYYMMDLQTGQEIHFLINNKQEITVEPDVAFTASSTIKLPILTSYLI
                     NHGTNLDTATTEAITRVFRISDNSATDSILERIDPNNGPIIVSQNMEQLGLTSTYISG
                     MFFLGAPNLIPGKVTPGNSRADIFTDPDPYSQTTPSEMGTLLADIYQCVQNGGGALIA
                     AFPDKVTPDTCKLIIDFLALDKFGALIQGGVPDGTLVPHKHGFVLSRDGVMHDISDVG
                     IVYSPGGNFILSIYTYHPVQNIWDITNPLFVNLTQATYNYFNIAAQ"
BASE COUNT          564 a          608 c          467 g          567 t
ORIGIN      
        1 caagttgatt gttttatccg tatacggcgt atcttccata tcaacgcgga caaaggtttt
       61 agcggtttta gcacgagcga ggatgcgctc caaggtttca gcacacaggc tctcgtccag
      121 ccccatgccg atttgtgtca gcttaatgga aatattgctg cgaaggctag ggtccgctcc
      181 aatagcatcc aatgttgcga agatactatc cgctgcctgc tgcgcctctt caggggtatt
      241 cgtatgctcc cctaagtgat ccaaagtagc attgattccc tttgcattta actcatggac
      301 aattttgatt gcatctgtaa gggtaacccc cgcaacaaaa cgagatgcga tccgccagga
      361 aaagctccaa cctgtgatca gcttttgtgc ccaggcggct ttcgaaaggt agataaaaaa
      421 tgaacggagc atatccatcc tttgaactgt gatttagatt aatatcaact tattctagca
      481 caagccctat gtttcggtat gttatacttt ttccgtgatt tttgtcatgc ccatgacatc
      541 ggaggcaatg tttaaccgtg agaaaccgaa ataccaatac catccttcgg ggtgtttcga
      601 tcttattttt actcggggcg ttcacactaa ccgtcgtttc ccttatctct tatagccgtc
      661 aaaggacggg ctaccccgcc ggcatgatga ttgccggtgt tccagtagat ggcttatccc
      721 ctgccgaagc atctcaaaga cttctccaag tgtacaactc gccaattgaa gtaaattata
      781 acggcgcgtt ttttcacatc gacccaggag cagtcggctt caccatggat gttgagacca
      841 tgctggcagc cgcagatctg acaagaacag gcggctcttt ctggggcgaa ttttgggact
      901 acctctggaa ccgagcgcca gccgaatctg aaatcccgtt aagtgcaagc atatccgaag
      961 accgtctgcg agaatttcta cagaatgaaa ttgcatcgcg gtacgatatc ccccccgccc
     1021 ccgcacaacc cgtccccggc gggacaacct tcctccccgg cgctcccggg cagactcttg
     1081 atgtcaaccg tgctgttgtg atcatcacaa acgctttgcg ctctcctgca gaccgctctg
     1141 ttgtattgac gttcaaccaa accaccgcat cacgcccaac catcgaaaat cttgaaatcc
     1201 tgctcaaaca aatcatcacc acatcagact ttgacggttt gattggttac tacatgatgg
     1261 acttgcagac tgggcaggaa attcactttt tgatcaacaa caaacaagag atcacagtag
     1321 aaccagacgt tgctttcaca gcatccagta cgatcaagtt gcccattctg acttcatacc
     1381 tgatcaatca tggcacgaat ctcgatacag caactacaga agccattacc cgtgtgtttc
     1441 gtatttcaga caactctgct acagattcca tccttgagcg tatcgaccca aacaacggtc
     1501 cgatcatcgt ctcccaaaat atggaacaac tcgggctgac cagtacctac atcagcggta
     1561 tgttcttcct cggtgcacca aacttgattc caggaaaggt cacccctggc aattctcgcg
     1621 cagacatttt cacagaccca gacccctatt cgcaaacaac tccgtccgaa atgggcacct
     1681 tgctagcaga catctaccag tgcgtccaaa atggaggcgg ggcgttaatc gcagccttcc
     1741 cagacaaagt cactccagac acttgcaagc tcatcatcga cttcctcgca ttggataaat
     1801 ttggcgccct cattcagggc ggcgttccag atggcacgct cgtcccccac aagcacggat
     1861 ttgtgctctc gcgtgatggc gtcatgcacg atatcagtga cgttggcatc gtgtactctc
     1921 cgggagggaa tttcattctc tcgatttata cctatcatcc ggtgcaaaac atttgggata
     1981 ttacaaaccc gttgttcgtc aatctcacac aagcgacgta caattacttc aacatcgcgg
     2041 cgcaataacc caaacacccc gttctttcat gaacggggtg tttttatata ctttccattt
     2101 caaaaaaggg cattttaaga ctgtgccggg tataatttga tttatgagtg catcgctggg
     2161 acttttccgc cttcaacaag tagaccgtca gattgaccgc gccaaa
//
LOCUS       DAOQFT010000436         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33142_length_2194_cov_6.636746, whole genome shotgun sequence.
ACCESSION   DAOQFT010000436 DAOQFT010000000
VERSION     DAOQFT010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33142_length_2194_cov_6.636746"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..729
                     /locus_tag="PLR93_10465"
     CDS             52..729
                     /locus_tag="PLR93_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNH27435.1"
                     /translation="MDKSRYTLITFDVYTALFDIETSLTPLVRDTLKIEALSFVRAWR
                     SKQLEYALISNSLGATRIPFEALTRRSLDDTLFRHMLNPSEVIRTELVHAWHHLCPWQ
                     ESAKVVQSLHERGYTLGLLSNGDHALLDELSKTFPPVFEHIFSSEEAGYYKPHPSIYA
                     LPFQRLGIPATSLLHVAGSPTDVLGAKSAGLQCVWSNRLGQPYLDVNHKADYELKDLS
                     GLLDILK"
     gene            complement(733..1503)
                     /locus_tag="PLR93_10470"
     CDS             complement(733..1503)
                     /locus_tag="PLR93_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27436.1"
                     /translation="MSKDITRQVANALSVVLALTVNILASTLPLNGQNTGEISDRFLV
                     YFVPAGYVFAIWGIIYIGWIAFTIYQFQPAQKENPRLQNLGYFFALSGLFNAAWLVCW
                     HYNWFGLSVLVMLTLLSLLVVSYLKLNVGLTPVSTAEKWSVDIPFSVYLGWVSVATVA
                     NISDWLYFVNWNGFGIAPQAWAVIMIAIASVLGVLMTVTRRDSGYAFVLVWSFAGIAV
                     KQAGVPFVANAAWGATLFVFGLAFYSILVRRRRNDLTS"
     gene            complement(1625..>2194)
                     /locus_tag="PLR93_10475"
     CDS             complement(1625..>2194)
                     /locus_tag="PLR93_10475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH27437.1"
                     /translation="VMEMLSGPDLNHLIRQEKRISLSNTVSLLRGIADALDYAHLNGL
                     VHRDIKPSNVMLDSSTIPSRSVLTDFGIAKMLDAHTRITNTAGMLGTFDYMAPEQIQA
                     SADVDGRADIYALGVMTYQMLTGRLPFERPDTGAILLAHLNAPPPDARELIHDLPRSA
                     ARAVQKAMAKDVRDRFASAREFIGALETD"
BASE COUNT          626 a          529 c          505 g          534 t
ORIGIN      
        1 tcgttcagga atttcgaaaa tctcaaatcg cggcattgag aaaatctttc tatggataag
       61 agtaggtata cgttaatcac cttcgacgta tacaccgcgc tcttcgatat tgaaactagc
      121 ctcacaccac ttgttcgcga tactttgaaa atagaggcat tgagttttgt ccgcgcatgg
      181 cgcagtaagc aattggaata tgcccttatc agcaattcac tcggcgctac gcggattccc
      241 tttgaagcac tcacacgccg ctcgttagat gacaccctct ttcgtcatat gcttaaccca
      301 agcgaagtta ttcgcacaga actcgtccat gcatggcatc atttatgtcc atggcaagaa
      361 tcagcaaaag tagtccagtc tctgcatgag cgcgggtaca cccttggtct gctctcaaac
      421 ggcgaccatg cactattgga tgaattgagc aagacgtttc ctcccgtttt tgagcatatc
      481 ttctcgtcgg aagaagcggg ttattacaag ccacatccca gcatctatgc cttgccattt
      541 caacgcttgg gcattccggc aacatctctg ttgcatgttg ccggctcccc aacagatgtg
      601 cttggagcaa aatctgcggg cttgcagtgc gtttggtcta atcgtctcgg tcaaccgtat
      661 ttggatgtta atcacaaagc tgattatgag ttaaaagact taagtggatt gttggatatt
      721 ttgaaataag ggtcaagagg ttaaatcatt gcggcggcga cgcactaaga tcgaataaaa
      781 agccagcccg aatacaaaga gagttgcgcc ccaagcagca tttgctacaa aaggaacacc
      841 tgcttgttta acagcgattc cagcgaaaga ccaaactaac acgaaggcgt atccactgtc
      901 tctgcgtgtg accgtcataa gaaccccaag cacactcgca atggcgatca taatcacagc
      961 ccatgcctgc ggcgcaatgc cgaatccatt ccaattgaca aaatatagcc aatcagaaat
     1021 atttgcaaca gtggcaacgc tcacccaacc aagatacacg ctaaaaggaa tatccacact
     1081 ccatttttcg gcagtactga caggtgtaag ccctacatta agtttgagat aactaacgac
     1141 aagcagagaa agtaaggtaa gcatcaccaa tacgctcaac ccgaaccagt tgtaatgcca
     1201 gcacaccaac catgccgcgt tgaaaagtcc gcttaaggca aagaagtagc ccaggttttg
     1261 caagcgtgga ttttcttttt gtgcgggttg aaattgataa atggtgaagg cgatccaacc
     1321 aatgtaaata atgccccaaa ttgcaaagac atacccagca gggacaaaat atacaagaaa
     1381 gcgatctgag atttcgccgg tgttctgtcc gttaaggggc aaagtgctgg caagaatgtt
     1441 gacggttaat gcaagaacga ctgaaagtgc attggctact tgacgagtta tatctttaga
     1501 catgtctctc tccattattg atgtactgag atggatagga ctacagctat gtctcttact
     1561 gtcaaatact atcagagcgg cagaaaagaa aaacatgctt ggggagcatg ttttataaaa
     1621 gtctttaatc ggtttcgagg gcgccaataa attctcttgc agaggcaaac cggtctcgca
     1681 catcctttgc cattgccttt tgcacagcac gagccgccga gcgtggcagg tcgtgaatca
     1741 actcgcgcgc atcgggcggc ggtgcattta agtgcgccag caaaattgca ccagtgtcgg
     1801 gtcgttcgaa tggcaagcgc ccggtcagca tttggtaggt catcacaccc aaagcgtaaa
     1861 tatctgcgcg tccatctaca tcggcagacg cttgaatttg ttctggcgcc atataatcaa
     1921 aggtgccaag catgcccgct gtattggtaa tgcgagtatg agcgtccagc atcttggcaa
     1981 tgccaaaatc ggtcaacacc gaacgtgaag ggatagtgga agaatccaac attacattcg
     2041 acggcttgat atcacggtga accaatccgt tgaggtgagc ataatccaac gcatcggcga
     2101 ttcctcgcaa caggctcaca gtgttggata aagaaatgcg cttctcttgc cggatcagat
     2161 ggttgaggtc tggacccgaa agcatttcca tcac
//
LOCUS       DAOQFT010000437         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33195_length_2192_cov_8.287319, whole genome shotgun sequence.
ACCESSION   DAOQFT010000437 DAOQFT010000000
VERSION     DAOQFT010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33195_length_2192_cov_8.287319"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLR93_10480"
     CDS             <1..435
                     /locus_tag="PLR93_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNH27438.1"
                     /translation="SGNFLYPALHKAGFANQPTSESRSDGLALKDMYITASGRCAPPD
                     NKPSPEELNNCQPFLERELEIINPKVIVCLGRIAFERILKIYSEKSSVYKFAHGASYQ
                     LANGKWLLCSYHPSQQNTSTKKLTPTMFDAIWVKAKQLADRN"
     gene            441..1187
                     /locus_tag="PLR93_10485"
     CDS             441..1187
                     /locus_tag="PLR93_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH27439.1"
                     /translation="MKKINWKIVAAVITAVLLVLVAGFVFWASDASAATDVALQALNS
                     DSKVNVSAENGWVIFTPAENSRPETGFIFYPGGKVDYRAYAPILKMISAKGYFVAVVP
                     VPLNLAFFDINAASRVEAAYPEVKNWFVGGHSLGGVAASSYAASHDEIRGVVFWASYP
                     ADDSLKTKNIPAVSIYGTNDGLATGDKLDASKALLLEDTKFVPIDGGNHAQFGSYGPQ
                     AGDNEATISPEDQWIQATDATVAFFAEVLK"
     gene            1206..2174
                     /locus_tag="PLR93_10490"
     CDS             1206..2174
                     /locus_tag="PLR93_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH27440.1"
                     /translation="MKRWKKILLFAFAFACVTTTLGPFAVPIPKMDGLVSEQEFVEPD
                     SKFIEINGVEIHYKEKGSGERTFILLHPFGGSTYSWRNVMDEFAKYGKVIAYDRPAFG
                     LTERPMPGDWETNPYGMKANVEILREMLDAFGIEKAVLVGNSAGGGVAVAFALEYPER
                     IDELILVAPGVGGGYGPQFPAWALPIMWTPQMRHLGPLMMRDYQESLPRTVEREWFDQ
                     SKLTDEIRAKHLQILTIKNWDKAFYELTFAPAYPELRPLLPNLKVPALIVAGQEDRLI
                     RSWYFEAISTEIPEAQLKTIPQCGHVPQEECPTEFMEVVLKYLHYQ"
BASE COUNT          558 a          491 c          553 g          590 t
ORIGIN      
        1 tcggggaatt ttttgtatcc ggcgttgcac aaggctgggt ttgcgaacca gcccacgtct
       61 gagtctcgaa gcgatggact tgctctaaag gatatgtata tcaccgcgtc gggtcgttgc
      121 gctccgccag ataataagcc gagtccagaa gagctaaata attgtcagcc ttttcttgaa
      181 cgtgaattag aaattattaa tccgaaagtt attgtttgcc tgggtcggat agcttttgag
      241 aggattctaa aaatatattc cgaaaaaagt tcggtatata aattcgctca cggcgcgtct
      301 tatcaactgg caaatgggaa gtggctgctt tgttcatacc accccagcca gcaaaatacc
      361 tctacaaaaa aactgacccc aacgatgttc gatgcgattt gggtgaaggc aaaacaactc
      421 gcagatagga attgatgtgc atgaaaaaaa taaattggaa gatcgtagcg gcggtgatca
      481 ccgccgtttt gttggtactt gttgccggtt ttgtgttttg ggcaagtgat gcttcagccg
      541 ccactgatgt ggctttgcaa gcattgaact cagattcgaa agtgaatgtt tcggctgaga
      601 atggctgggt gatttttacc ccggcagaaa attcacgacc tgaaacgggc tttatttttt
      661 accctggtgg taaagtggat tatcgcgcat atgcgccgat cttgaaaatg atttctgcca
      721 aggggtattt cgtagcggta gttccggttc cattgaattt ggctttcttt gatatcaatg
      781 cagcttctcg ggtagaagcg gcgtatcctg aagtcaagaa ttggtttgta ggcgggcact
      841 ctttgggcgg cgtggcggca tcttcttatg cggcaagtca cgacgaaatt cgaggtgtgg
      901 tgttttgggc ttcgtatccg gctgatgatt ctttgaagac caagaacatc cctgcggttt
      961 cgatctatgg cacaaatgac ggacttgcaa caggcgataa gttagatgca tccaaagccc
     1021 tgctgttgga agatactaaa tttgttccga ttgatggtgg aaaccacgct caattcggct
     1081 cttacggacc tcaagccggc gataacgaag cgactatttc tcccgaagac cagtggattc
     1141 aagccaccga tgcaaccgtt gctttctttg ctgaggttct gaaataaatc gtctatcgtc
     1201 taaccatgaa gcgctggaaa aaaattctgt tattcgcttt tgcttttgcc tgcgttacca
     1261 ccacgctggg accttttgcc gtccctattc caaaaatgga cggtttggta agcgaacaag
     1321 aatttgttga gcctgatagt aaattcatcg agatcaacgg cgtggaaatt cattacaagg
     1381 aaaaaggttc tggcgaaagg accttcatcc tgttgcatcc ttttgggggc agcacgtatt
     1441 cttggcgaaa tgtaatggat gagttcgcaa aatatggcaa agtcatcgcg tacgaccgcc
     1501 cagcctttgg gctaacagaa cgtcccatgc ccggtgactg ggaaacaaac ccatatggca
     1561 tgaaagccaa tgtcgaaatt ttgcgtgaaa tgctcgatgc ctttggcatt gaaaaagctg
     1621 tccttgtggg caactcggcg ggcggtggtg tagcggtagc ctttgcgttg gaatacccag
     1681 aacgtatcga tgaactcatc cttgtagcgc ctggcgtggg tggtgggtat ggtccgcagt
     1741 tcccggcgtg ggcattgccg attatgtgga ctccgcaaat gcggcatctc ggtccgctca
     1801 tgatgcgtga ttatcaagaa agcctgccgc gtaccgtgga acgtgagtgg ttcgaccaat
     1861 ccaaactcac cgatgagatt cgtgcgaaac atttgcaaat tctcacgatc aaaaattggg
     1921 acaaagcctt ttacgaactc acctttgcgc cagcgtaccc tgaactacgc ccattgctgc
     1981 cgaacttaaa agtccctgcc ctcattgtag ctgggcagga agatcgtctc attcgctcgt
     2041 ggtattttga agccatctcc actgaaattc ccgaagcgca actcaagacc attcctcaat
     2101 gcgggcatgt gccacaagag gaatgtccaa ccgaatttat ggaagttgta ctaaaatatt
     2161 tacactacca atgagtactc tattgctgca aa
//
LOCUS       DAOQFT010000438         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33308_length_2188_cov_2.951711, whole genome shotgun sequence.
ACCESSION   DAOQFT010000438 DAOQFT010000000
VERSION     DAOQFT010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33308_length_2188_cov_2.951711"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLR93_10495"
     CDS             complement(<1..1089)
                     /locus_tag="PLR93_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNH27441.1"
                     /translation="MAFVPQLLFVLVLASAAFIIRRRVLRIRDNIRLGKSNPVNDCTG
                     ERWSNVLLVAFGQKKMFKKMIPAVLHFFIYAGFLIINLEVLEFVIDGLAGTHRIFAPY
                     LGSVYNLLMNFFEFLALAVLLACVVFLLRRNVVRVPRFRSPELTGWPLLDANLILITE
                     IVLMTAIFTMNACDQLLQTQSEHYTATGTLFFSSFLMPLFENMSLDALHLIERSAWWF
                     HILGILGFAIYVTYSKHLHIFMAFVNTYYAKLEPKGQLNNMPVVTREVKSMLGIAPAD
                     AAPAGEPGRFGAKDINDLTWNQLAAAYSCTECGRCTAECPANLTGKKLSPRKIMMDVR
                     DRMEEVGNSIASGGPGLQVRQAGFGTSEC"
     gene            1232..1304
                     /locus_tag="PLR93_10500"
     tRNA            1232..1304
                     /locus_tag="PLR93_10500"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1265..1267,aa:Phe,seq:gaa)
     gene            complement(1312..>2188)
                     /locus_tag="PLR93_10505"
     CDS             complement(1312..>2188)
                     /locus_tag="PLR93_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNH27442.1"
                     /translation="KEAERQGIEIKLMSGVEEVTPHARGFAVRCSSGTVYEARKILVA
                     QGGHARPDAYQWIQKLGHHIVSPIPSLFTFNNPSRQFTELMGVAVPDAIVKISGTKLE
                     SRGPVLITHWGLSGPAVIRLSAWAAQHLHDVGYTFQAMVNWTGEQTETSVREVFQSQR
                     EGKKKVITNPLFGLPQRLWEKLCTMAEVSPEFIWPDMPVKVQNRLMEYIIRCPFSFRG
                     KTTFKEEFVTCGGVDLGELNTATLESKLIPGLYFAGEVLNIDGETGGFNFQAAWTTAF
                     VAARDLTGANKKALD"
BASE COUNT          571 a          556 c          537 g          524 t
ORIGIN      
        1 acactcgcta gtgccgaagc cagcctggcg gacctgcagt ccggggccac cgctggcaat
       61 actgttgccc acttcttcca tgcggtctcg aacatccatc atgattttgc ggggtgacaa
      121 tttcttcccg gtaagattgg ccgggcattc cgctgtacaa cgtccgcatt cagtacatga
      181 ataagctgct gccagttgat tccacgtaag gtcattgatg tctttggcac cgaaccgccc
      241 gggctcgcct gccggagcag catcagcagg agcaataccc agcattgatt ttacctcccg
      301 cgtcaccacg ggcatgttgt tcagctgacc tttaggttcc agtttagcgt agtaggtatt
      361 cacaaacgcc ataaaaatgt ggaggtgttt ggaataggta acatatatgg cgaagccgag
      421 aatgccaagg atgtgaaacc accatgcaga tcgttcgatc agatgcaggg catccaaact
      481 catattctcg aacagtggca taaggaatga actaaagaac aatgtgccgg tggctgtata
      541 atgttcagac tgtgtctgca acagttgatc gcaggcattc atcgtgaaga ttgccgtcat
      601 caagacgatt tccgtaatga gaatcaggtt ggcatccagc agtggccatc cggtaagttc
      661 gggcgaacga aatcggggta ccctgaccac attgcgacgc aggagaaaga ctacacaagc
      721 gagcaacaca gccaacgcga gaaattcgaa gaagttcatc agtagattgt acaccgaacc
      781 cagataaggg gcgaagatgc ggtgcgttcc ggccaggcca tcaattacaa attcaagcac
      841 ttcaagattg atgatgagaa acccggcata aatgaaaaag tgaagcacgg ccgggatcat
      901 cttcttgaac atcttctttt gtccaaaggc aacgagaagg acattgctcc atctttcgcc
      961 ggtgcaatcg tttaccggat tggacttgcc taagcggata ttatccctga tccgaagcac
     1021 ccgcctgcgg atgatgaaag cggcagaagc cagaacaagg acaaagagga gctgcggaac
     1081 gaatgccatg gaaaggagtt aacgattcaa attaatcaac aaaatcggga aaatgtgcgc
     1141 cattcggggt ttgtgaacgt tgataatttg caccatttct aattttatct acttttgtgg
     1201 cccgtctgct gaaagctgac tttttatacc cggtgacgta gctcagttgg tagagcaaag
     1261 gactgaaaat ccttgtgtcg ggggttcaac tccctccgtc accacaagcc ctcaatcgag
     1321 ggctttttta ttggcgcccg tgaggtctct tgcggcaaca aatgccgtag tccaggcagc
     1381 ctgaaaattg aagcctcctg tttcgccatc aatattcaac acttcacccg cgaaatataa
     1441 gccgggtatc aacttgctct caagtgtagc ggtgttcagt tcaccgagat caacgccgcc
     1501 acaagtgacg aactcttctt tgaaggttgt tttgccccga aatgaaaagg gacatcggat
     1561 gatgtattcc atcaaccggt tttgcacttt gaccggcatg tccggccaaa tgaactcagg
     1621 actgacttct gccatcgtac acaatttctc ccatagccgc tgaggcaatc cgaacaaagg
     1681 attggtgatt acttttttct ttccctcccg ttgcgactga aagacttcgc gcacagatgt
     1741 ttcagtttgt tcaccagtcc agttcaccat agcttgaaat gtgtacccca catcatggag
     1801 atgttgcgct gcccaggcag ataaccggat aaccgcgggt ccgctcagtc cccaatgagt
     1861 gattaacacc ggcccgcgag attccagttt ggtgccggag atcttcacga tggcatccgg
     1921 cacggcaaca cccatcagtt cggtaaactg gcgcgatgga ttattaaatg taaagagcga
     1981 tggaatggga gaaacaatat ggtgacccag cttttgaatc cattgatagg catccggtcg
     2041 ggcatggcca ccctgtgcga caagtatttt tctagcttca tatacagtgc cggaactgca
     2101 gcgcacggcg aatcctctgg catgaggcgt tacttcttca acaccactca tgagtttgat
     2161 ctcaattccc tgccgctcgg cctccttc
//
LOCUS       DAOQFT010000439         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33354_length_2186_cov_6.329423, whole genome shotgun sequence.
ACCESSION   DAOQFT010000439 DAOQFT010000000
VERSION     DAOQFT010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33354_length_2186_cov_6.329423"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2186
                     /locus_tag="PLR93_10510"
     CDS             <1..>2186
                     /locus_tag="PLR93_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNH27443.1"
                     /translation="LLPWSPSVPPQWSGENYPANLESGVVNLAHILAGSEGTLAVIRK
                     LKVNLVQKPKHTILGILSYTSIVEACDDVPRLLAMKPSAVELIPQLILRNARSIPAYA
                     RQMGWVIGDPAAVLVVEFSGDQPSALKEAVRKLGDVLTIAESPEDQARIWNIRKVGLG
                     ILDSRPMSARPIAFIEDCAIPVERLGKFVREVEQILNAHGTFGGIYAHASAGCLHIRP
                     VLDLQLGEAVRNMRNIIEQVFALTMSLGGAMSSEHGDGIARGEFIERTYGREVTEAMR
                     LLKKAADPDNLLNPKKLFDAPPMDTNLRYGETYQAQAWKPVLTFEHERGLEGAVESCN
                     GQGVCRKTNGVMCPSFQATRDEAFSTRGRANLLRALMRSDVDFSVPRNSELELATYQS
                     LDLCLACKGCTSECPSGVDMPKLKYEFMNQYYKSHPRHLRDYLFGYFHVATKLLMPFA
                     PMANWFMQIGWSKKIVARVLGISEHRPFPKFVRGTRQAHEMQFDKQVIFVSDVFSHYI
                     EPEVEEAVVEILNSLGYEVKILPTIGAGASLLSKGFLNAAQRHAEKVLAEIKRLDGGA
                     GLSVVGCEPPELYCLKHEYESLLPNRKEEIESIKKRTWLVDEFILRVVNEAKMDISHV
                     ASSEQNNRLTLHPHCHHRAEGPAADGLPSGVSATVDMLKQFGYEVDVIDAGCCGMAGT
                     FGYDVEHYELSMKVGELGVLPKVRDKKLENRELVASGAACRMQIKQ"
BASE COUNT          558 a          418 c          603 g          607 t
ORIGIN      
        1 tcttttgcca tggagcccat ccgtgccgcc gcaatggagt ggagaaaact acccggctaa
       61 cttggaatct ggcgtagtga acttggcgca tatattagca ggcagcgaag ggactttggc
      121 ggttatccgt aagttaaaag ttaaccttgt tcaaaagcct aagcacacga ttcttggaat
      181 tttgtcgtat accagtatcg tagaagcgtg tgatgatgtt ccgcgtttgc tggcaatgaa
      241 gcctagcgca gtggagttga ttccgcagtt gattctgcgg aatgcacgta gtattccggc
      301 gtatgcgcgg cagatgggtt gggtgattgg agacccggca gccgtgttgg tagtggaatt
      361 tagtggcgac caaccgtcgg ctttgaaaga agcggtgcgt aagcttggtg atgttttgac
      421 tattgcagag tcgcctgaag atcaggctcg catttggaac atccgcaaag tggggttggg
      481 gattttagat tcacgcccaa tgtctgccag accgattgca tttattgaag attgcgctat
      541 ccccgttgaa aggttgggga agtttgttcg tgaagttgaa cagattctga atgctcatgg
      601 gacttttggc ggaatttatg cacatgcttc ggcaggctgt ttgcatattc gccctgtgtt
      661 agatttgcaa ctgggtgaag ctgtgcggaa catgcgaaac attattgagc aagtttttgc
      721 gttgaccatg tctttgggcg gtgccatgag cagcgaacat ggcgatggaa ttgcgcgtgg
      781 ggaattcatt gaacgaactt atgggcgtga agtgactgaa gccatgcgct tgctgaagaa
      841 agccgcagac cccgataatc ttttgaaccc taaaaaattg ttcgatgcgc cacccatgga
      901 tacgaactta cggtatggcg aaacgtatca ggcgcaggct tggaagcctg tattgacctt
      961 tgaacacgag cgaggtttgg aaggtgcagt tgagtcatgc aatgggcaag gcgtatgtcg
     1021 taaaacgaac ggtgtgatgt gcccatcgtt tcaggcaacg cgcgatgaag cctttagtac
     1081 gagagggcga gctaatttac tgcgggcatt gatgcgctcg gacgttgatt tttcagtgcc
     1141 tcgaaattct gaacttgaat tagcaaccta tcagtcactc gatctttgcc ttgcttgtaa
     1201 aggctgcaca tccgaatgcc caagtggtgt ggatatgccg aaactcaaat atgagtttat
     1261 gaatcaatat tacaaatcgc atccacgcca cttgcgagat tatctgttcg gctattttca
     1321 tgtcgctaca aaattgttga tgccatttgc ccctatggca aattggttta tgcaaatagg
     1381 ctggtcgaag aagatcgtgg cacgtgtgtt gggtatttca gaacacagac cttttccaaa
     1441 atttgttcgg ggtacacgcc aagcgcatga aatgcaattt gataaacaag taatttttgt
     1501 ttcggatgtt ttctcgcatt atattgaacc tgaggttgaa gaggctgttg ttgagatttt
     1561 gaactcgttg gggtatgaag tgaaaatttt gccaactatt ggagcaggcg cttctttgct
     1621 ttcgaaggga tttctcaatg ctgcacagcg tcatgcagaa aaagttttag cagaaattaa
     1681 acgcttggat ggtggagcag gtttgagcgt agttggatgc gagccgcccg agttgtattg
     1741 tctcaaacat gaatatgaat cgttattgcc aaatcgcaaa gaggaaattg aatccataaa
     1801 gaagagaacg tggttagtgg atgaatttat tttgcgtgta gtcaatgagg ctaaaatgga
     1861 tatttcacat gtggcttctt ctgaacaaaa taaccgcctc actctccacc cgcactgtca
     1921 ccaccgcgca gaaggtcccg ccgctgatgg tttaccttct ggcgtttcag ctacagtaga
     1981 tatgctcaag caatttggct atgaagtgga cgtaattgat gcaggttgtt gtggcatggc
     2041 aggcaccttt ggctatgatg ttgaacatta cgagctatca atgaaagtag gggagttggg
     2101 tgtgttaccg aaagtgagag ataagaaatt agaaaacaga gaattggtgg cgagcggtgc
     2161 ggcttgtcgt atgcagatta agcaag
//
LOCUS       DAOQFT010000440         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33432_length_2183_cov_8.000470, whole genome shotgun sequence.
ACCESSION   DAOQFT010000440 DAOQFT010000000
VERSION     DAOQFT010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33432_length_2183_cov_8.000470"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLR93_10515"
     CDS             <1..703
                     /locus_tag="PLR93_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27444.1"
                     /translation="QRDIPRATILALIIQGLFSYLFGYFIAGFALNTQIDATASQAPM
                     GDIALQIGDLLLDGYGATLMVFFGFTVAIALLGGTLTAINNGVRISFAMALDEEMPDI
                     FGMLHPRYTTPYNTVILLSIVCAIIGSAGIIGGMPVLMGIVLASNLGAFLLYALLCLL
                     TVVTFNGDPAFSFLRHLLVPGIGFLVNVGTVILAAKIGFSAGGLISQASWVAWGLAGV
                     WFIVSIIYYFLRRKA"
     gene            837..1667
                     /locus_tag="PLR93_10520"
     CDS             837..1667
                     /locus_tag="PLR93_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNH27445.1"
                     /translation="MRKFIPEIESSGLSIIGPVREDNQDTIHITDYPNSNGAGFLFAI
                     ADGMGGYAHGGMASALALESFLNVILTHHRQPVLRTLQRGVETANLHVYQKARQMDAG
                     RMGTTLTAAYILGDMLYLTHVGDSRAYLVRDGQATCLTSDHTTVGDMVRAKLIPAAKI
                     RTHAQRSILTKSIGTDLFVQPDILQQKLKPGDCLILCSDGLWSVVEDDDFARVASQSS
                     SVSEVSQNLVDLALAHHTDDNVSVVTFSVRSFMPVPAEQEPLPEESLGWFKKLRKMVS
                     "
     gene            1694..>2183
                     /locus_tag="PLR93_10525"
     CDS             1694..>2183
                     /locus_tag="PLR93_10525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH27446.1"
                     /translation="MLQIGDQFDHYQIQAHMAQGGMSDVYRAFDLMHRREVVIKVPDH
                     SMIGDPAQFERFQRELEVMKSLSHPAILKGLDSGKHNRVPYLVTEFVQGESLRTLMEK
                     SGSLPTDQVVSLIQKLANGMAYCHKNEVIHRDLKPENILITSDGQPVIMDFGLALTKD
                     SHR"
BASE COUNT          548 a          556 c          486 g          593 t
ORIGIN      
        1 ccaacgcgac attccgcgcg cgacaattct ggcgttaatc attcaaggct tattctcata
       61 tctttttggt tatttcattg caggattcgc acttaacacc caaattgatg ccactgcatc
      121 acaagcgccc atgggcgata ttgccctaca aatcggcgac ttgttattag acggttatgg
      181 tgccacctta atggtatttt tcggctttac cgtcgccatc gccctcctcg gtgggacatt
      241 aaccgccatc aacaacggcg tacgaatcag cttcgccatg gcgctggatg aagagatgcc
      301 agatattttt ggcatgttac atcctcgata caccacgcct tataacaccg ttatattact
      361 cagcattgtc tgcgccataa tcggctctgc cggaatcatt ggcggcatgc ccgtattaat
      421 gggcatcgtt ctggcatcca atctcggcgc gtttctactt tatgctctgc tctgtctgtt
      481 aaccgttgtc acattcaacg gtgacccagc attcagtttt ctgcgccatc ttctcgtccc
      541 ggggatcggg ttcttagtca atgttggcac tgtgatcttg gcagccaaga ttggtttctc
      601 tgccggtgga ctcattagtc aagcaagttg ggttgcttgg gggctagctg gcgtatggtt
      661 tatagttagt atcatctatt acttcttgag acgaaaagcc taaacgccaa ctgaaatgcc
      721 ccatttgggt actattttca aacaaccagc ttacatagaa ttgcttaatc tttatttcct
      781 cacagcaggg attgattttg cctgctgtga gtggactact gtgaaagcga ctggttttga
      841 gaaaattcat ccccgagatc gaaagtagcg gcttgagcat tataggtccc gtacgagaag
      901 ataatcagga tacgattcat atcactgatt atccgaactc caatggggct gggtttctct
      961 ttgccatcgc agatggtatg ggtggctatg ctcatggggg catggcaagc gcattagctc
     1021 ttgagtcctt tctaaacgta attctcacac atcatagaca gcctgtttta cgcaccttac
     1081 agcgcggagt tgaaaccgcc aacttgcatg tctatcaaaa agcccgtcaa atggatgcag
     1141 ggcgcatggg caccacactc actgccgcct acatattggg agatatgctg tacctcacac
     1201 atgtaggaga tagccgtgcc tatctcgttc gcgacggtca agccacttgc ctgacatcag
     1261 accacaccac cgtaggagac atggtccgcg caaaattgat tcccgccgcc aagatcagaa
     1321 cccatgctca gcgctccatc ctcaccaaat ccataggaac cgatttattc gtacagcctg
     1381 atatcctcca gcaaaagctc aaaccaggcg actgcctcat cctatgctca gatggattat
     1441 ggtcagttgt ggaagatgat gatttcgcaa gagtcgcatc ccaatcctca tctgtttctg
     1501 aggtgagcca aaatctagtc gatctagcac tggcgcatca caccgatgac aatgtatctg
     1561 tggttacctt ttcagttcgc agctttatgc ctgttcctgc cgagcaagaa cctttacctg
     1621 aagaatcact tgggtggttt aagaagctaa ggaaaatggt gtcatgaatg cctatgctat
     1681 aatgacgaac actatgcttc aaatcggcga tcaatttgat cattatcaaa ttcaagcaca
     1741 catggctcag ggcggaatga gtgatgtata tcgcgccttt gatcttatgc accgccgcga
     1801 ggttgttatt aaagttcccg atcattccat gatcggcgac cctgcccaat ttgaacgctt
     1861 ccaacgtgag cttgaagtta tgaagtcact tagccaccca gcgattctaa aagggctgga
     1921 ttctgggaag cacaaccgcg tcccatatct tgtgacagaa ttcgtccaag gcgaatcatt
     1981 gcgcacgttg atggaaaaaa gcggctcttt accgaccgat caagttgttt ctctcatcca
     2041 aaaattagcc aacgggatgg catactgcca caagaacgaa gtcattcatc gtgacctgaa
     2101 acctgaaaat atccttatca ctagtgatgg acaacccgtc atcatggatt ttggtctcgc
     2161 gttgacaaaa gactcgcacc gtg
//
LOCUS       DAOQFT010000441         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33553_length_2178_cov_6.946773, whole genome shotgun sequence.
ACCESSION   DAOQFT010000441 DAOQFT010000000
VERSION     DAOQFT010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33553_length_2178_cov_6.946773"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PLR93_10530"
     CDS             <1..1474
                     /locus_tag="PLR93_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH27447.1"
                     /translation="SGGESSNLRDYDPATTYSAGDKLIFSGLVQLDQNLNIRPDLAET
                     WEVTGDGTIYTFKIRDNAKFHNGRDVTAQDVVFSWERAASPELASDTVLTYLSDIVGV
                     REKALGQADSISGLKVIDDKTLQVTIDAPKPYFLMKLTFPTSYIVDQDNVSAGEEWVR
                     QPNGTGPYKLKEWRSNEYIVYEANPDFYLGAPSIPYVVFKLYAGDDVRLFETGDVDIA
                     SVGLYDVERMLNPNEPLNRNLVTGVDLCTSYVTFDTTQPPFDDANVRKAFSMAFDRQR
                     YVDVLYRGVALPAEGLYPPGLPGFDYNLQGLPFDPEQARELLKQSKYGGPEGLPEIIY
                     TNAGIGSYIGGNVAALAEMWQDYLGVKIKVENLDYNFYYQQIFSGNHGQIFDGGWCAD
                     YPDPENFADVLFHTGVPQNQGGYSNPELDTLLEAARIEPDVTKRIAMYQEAERIIVND
                     APVIFTTHSLSYQLVQSYVKGYVNTPISISAERFMWLDGK"
     gene            complement(1504..>2178)
                     /locus_tag="PLR93_10535"
     CDS             complement(1504..>2178)
                     /locus_tag="PLR93_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27448.1"
                     /translation="NLIAANPDVNPNSLTIGQTLFIPDPSAPLAAASTPTPLPVPISQ
                     AACHPTADSGNWCFALIQNNTNSILENVFARITLLDKSGNALVYQTAYLPLDVIPTNA
                     SLPVYVYFPNTPADVQPQVQLLSAMTGGSAYLPISLDNTSAQMDWNGKTAKVSGQAYL
                     PAESNAATQVWIAAVAFDKYGTVVGVRRWEGGALQPGSSLNFDFSVSSVGGEIEAVEF
                     FGQARP"
BASE COUNT          537 a          537 c          551 g          553 t
ORIGIN      
        1 cagtggtggt gaatcttcaa atttgcgtga ttacgacccc gccacgacct atagcgcggg
       61 cgataaactc atcttcagtg gtttggtgca actagaccaa aacttgaaca tccgcccaga
      121 ccttgccgaa acttgggaag tgactggcga tggcactatc tacaccttca agattcgcga
      181 caatgccaag ttccataatg gacgggacgt tacggctcaa gacgtggtct tttcttggga
      241 gcgcgctgcc agccctgagt tggcgtctga caccgttctc acctacttga gcgatatcgt
      301 tggtgtacgc gagaaagcgc ttggtcaggc agatagtatc agcgggctaa aagttatcga
      361 cgacaaaacc cttcaagtca cgattgatgc gcccaagcca tactttctca tgaagctcac
      421 cttccccaca tcctatattg tggatcaaga taatgtatcc gctggtgagg aatgggtgcg
      481 tcagcctaat ggcacgggac cttataaact caaagaatgg cgctccaacg aatatatcgt
      541 ttacgaagcc aatccagatt tctacctggg tgcgccatcg attccttatg ttgtcttcaa
      601 actctatgca ggtgatgatg tgcgcctctt tgaaacgggc gatgtagata ttgcgagcgt
      661 cggcttatac gatgtagaac gtatgctgaa cccaaatgag cctttgaacc gcaatctcgt
      721 caccggtgtg gacctttgta caagctatgt tacctttgac accacccaac cgccttttga
      781 cgacgccaat gttcgcaaag cctttagcat ggcattcgac cgtcaacgct atgtggatgt
      841 gttatatcgc ggtgtggcat tgccagcaga ggggttatac ccgcccggtt tacccggctt
      901 tgattacaac ctgcaaggtt taccctttga ccccgaacaa gcgcgtgaat tgttgaagca
      961 atccaaatac ggcggacccg aaggcttgcc tgagatcatt tacaccaatg cgggtattgg
     1021 tagctatatt ggtggaaatg tagccgcttt ggctgagatg tggcaagact atcttggcgt
     1081 gaaaatcaag gtcgagaatt tagattacaa cttctactat caacagattt tttctggcaa
     1141 tcatggtcag atctttgatg gtggttggtg tgccgattac cccgaccctg aaaattttgc
     1201 cgatgttctc ttccataccg gcgtgccgca aaatcaaggc ggctattcca acccagagtt
     1261 ggacacctta ctcgaagccg ctcgcatcga accggatgtg accaaacgca ttgccatgta
     1321 tcaagaagcc gaacgcatca ttgtcaacga tgcgcctgtg atcttcacca cccattccct
     1381 ttcgtatcaa cttgtgcaat cgtatgtgaa gggctatgtg aatacaccca tctcaatctc
     1441 agcagaacgc tttatgtggc tggatggtaa atagacaacg gaccatagac gatggatcat
     1501 cgtctatggt cttgcttgcc caaaaaattc caccgcctca atttccccgc cgacgctgga
     1561 tacgctaaag tcgaagttga gcgaactccc cggctggagc gctccgcctt cccagcggcg
     1621 cacaccaacc actgttccat atttatcgaa tgccacagcc gcaatccata cctgggttgc
     1681 ggcgtttgat tctgcgggta aatacgcttg tccgctgacc ttggcagttt tgccattcca
     1741 atccatttgc gcggaggtgt tatccaacga gatgggcaaa taagcagaac cacctgtcat
     1801 cgcgctcaac aattgaactt gtggctgtac gtctgcgggt gtgtttggaa aatagacata
     1861 cacaggtagc gaagcattgg ttggaatcac atctaagggc agatatgcgg tttgatagac
     1921 aagggcgttt ccgcttttat ctaataatgt gattcgggca aagacatttt ccagaatgct
     1981 gtttgtattg ttttgaatga gtgcaaagca ccagttaccc gagtcggcgg tggggtgaca
     2041 ggcggcttga gaaatgggaa caggtaacgg cgtaggggtt gaggcggcgg cgagaggggc
     2101 agatgggtct gggatgaaga gcgtctgccc aatggtgaga ctgttcgggt tcacatccgg
     2161 atttgccgca atcaaatt
//
LOCUS       DAOQFT010000442         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33649_length_2175_cov_5.695283, whole genome shotgun sequence.
ACCESSION   DAOQFT010000442 DAOQFT010000000
VERSION     DAOQFT010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33649_length_2175_cov_5.695283"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1039)
                     /locus_tag="PLR93_10540"
     CDS             complement(38..1039)
                     /locus_tag="PLR93_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNH27449.1"
                     /translation="MTELSTTYLGLKLKNPLVASASPLSKKTDKAKKLEEAGISAIVM
                     YSLFEEQIIHESLELDHFLSRGTDSFAEALTYLPDGGMYSVSPEKYLNQVTSLKKAVN
                     VPVIGSLNGVSKGGWTSYAKRIEEAGADALELNLYFLSTDPNLTAAELETAQAELVAE
                     VKSSIKIPLAVKLSPFVTSLPNFAKRIVDAGADGLVLFNRFYQPDFDLDNLDIVHSLD
                     LSTSADLRLPLRWISILHSNVHTDYALTSGIHTAHDVLKAMMAGAKVAMMASNLLHSG
                     ETVVSSMLNDLKTWMKEREYESIQQMQGSMSQKNVKEPAAFERANYMKVLGSWRDLP"
     gene            complement(1102..>2175)
                     /locus_tag="PLR93_10545"
     CDS             complement(1102..>2175)
                     /locus_tag="PLR93_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH27450.1"
                     /translation="PYVKLVSQLFGDRAVIANATGCSSIYGGNLPTTPWAKNAEGRGP
                     AWSNSLFEDNAEFGLGMRLTIDKQKEYAEELLKSFEQTVGTQLVTDILHANQADEAGI
                     DAQRVRVEELKSKLAKSSDSRAKDLISLAENFVKKSVWIIGGDGWAYDIGYGGLDHVL
                     ASGRNVNILVLDTEVYSNTGGQSSKSTPRAAVAKFSMGGKGMPKKDLGLIAMSYGYVY
                     VAKVSMGANDQQTLKALLEAESYDGPSLVIAYSPCIAHGFDMARSLDQAKLAVQSGHW
                     PIFRYDPRLAIDGKNPLQIESKEPSIPFTQYAYNETRYKMLTQMNEERAEELMKEAQH
                     DAKTRWTLYQQMASMHYESDKKE"
BASE COUNT          498 a          513 c          571 g          593 t
ORIGIN      
        1 ctccatttgg agagcctatt ttctttttta ttatggatta cggcaaatca cgccacgaac
       61 caagaacctt catgtagttc gcacgttcga atgctgccgg ttctttgacg ttcttctggc
      121 tcatgctgcc ctgcatctgc tgaatggatt cgtactcacg ttccttcatc caggttttta
      181 agtcatttag catggaagag acaacggttt ctccgctgtg cagtaggtta gatgccatca
      241 tggcgacctt tgcgcctgcc atcatggctt tgagtacgtc atgagcggtg tggatgccgc
      301 tggtaagggc gtaatctgtg tggacgttgc tgtgcaaaat tgagatccag cgcagtggga
      361 ggcgtaagtc tgcagatgtg ctcaaatcca agctgtggac gatatccaga ttatcgaggt
      421 cgaaatcggg ttggtagaag cggttgaaga gcacaagtcc gtctgcaccg gcgtccacga
      481 tgcgtttggc aaaattaggc agagatgtca cgaagggact aagcttcact gccagcggaa
      541 tcttgatgga ggattttact tccgctacta attctgcttg ggcggtttca agttcggcgg
      601 cggttaggtt cgggtctgta gagagaaagt ataggttaag ttcgagcgca tcggctccag
      661 cttcttcaat gcgtttggcg tagctggtcc aaccgccttt ggagacgcca ttcaagctgc
      721 cgattacggg gacattgacc gctttcttca aactggtcac ctgattaaga tatttttcag
      781 ggctcacgct atacataccg ccatcaggta gataggtaag ggcttcagca aaagagtcgg
      841 tgccgcggga aaggaagtgg tctaactcaa ggctttcgtg aatgatctgc tcttcaaaga
      901 gagaatacat cacgatcgcg gagatgcccg cttcttctaa ctttttagct ttgtcggtct
      961 tcttagagag gggtgaagcc gaagccacaa gcggattttt tagttttaag ccaaggtacg
     1021 tagtggaaag ttcagtcatg ggtaaagttc ctttagggag ttaagaatgg gcgagaaacg
     1081 ttctcgccca taatttaaag tttattcttt tttgtcgctt tcgtaatgca tggatgccat
     1141 ttgttgataa agcgtccaac gggttttggc atcatgctgg gcttctttca tgagttcttc
     1201 ggctcgttcc tcattcattt gagtgagcat cttataccgg gtttcgttat aagcatactg
     1261 cgtgaatggg atgctgggtt cctttgactc gatctgaagc ggatttttac cgtctattgc
     1321 caagcgcgga tcgtaacgga agatgggcca gtgaccagat tgtacggcga gctttgcttg
     1381 gtcgaggctg cgagccatat cgaagccgtg tgcaatacac gggctgtatg caatgacgag
     1441 ggaaggtcca tcatacgatt cggcttccaa cagagctttc aaagtctgct ggtcgttggc
     1501 gcccatggac acctttgcaa cataaacgta gccgtacgac atggcgatca gaccaaggtc
     1561 tttcttgggc attcccttgc cgcccatgga gaacttggca actgctgcac gcggtgtgga
     1621 cttggaggac tgaccacctg tattcgaata cacttcggta tcgagcacga gaatgttgac
     1681 attgcgtcca ctggcgagga catggtccaa accgccgtag ccaatgtcgt atgcccaacc
     1741 gtcaccacca attatccaaa ccgatttctt gacgaaattc tctgccaaac taattaagtc
     1801 tttggcgcgg ctgtcgcttg atttcgcaag tttcgatttc agctcttcca cgcgaacgcg
     1861 ctgcgcatca atgccggctt catctgcttg gttggcatgc aaaatatcgg ttaccaactg
     1921 agtgcctacc gtctgctcaa aggacttgag caattcttcg gcatattctt tttgcttatc
     1981 aatggtgagg cgcatgccca aaccaaactc agcgttatct tcaaacaacg agtttgacca
     2041 agcaggtccg cgaccttcgg cattcttcgc ccacggagtg gtgggcaggt tgccgccata
     2101 gatggacgaa caacctgtgg cattcgcgat tacggcgcgg tcaccgaaca attgcgaaac
     2161 caacttgacg tacgg
//
LOCUS       DAOQFT010000443         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33734_length_2172_cov_4.960794, whole genome shotgun sequence.
ACCESSION   DAOQFT010000443 DAOQFT010000000
VERSION     DAOQFT010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33734_length_2172_cov_4.960794"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLR93_10550"
     CDS             <1..863
                     /locus_tag="PLR93_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27451.1"
                     /translation="GDFVAVDHINFEVNEGEVVGYLGPNGSGKTTTIRMLLGLLAPSE
                     GEATVLGYDVHRQSEEVRARVGYMSQKFAIYDDLTTLENLTFYGGVYGITDKARIMET
                     LELVGLSGHEHTLTRDLSTGWRQRLSLGIALVHKPKLLFLDEPTSGVDPTARRAFWDL
                     IYQLAESGVTILVTTHYMDEAEYCERVGVMRDGKLLAMDTPTNLKKSIITGDVWEVFA
                     HPLEDGLDALSDVDGIERVGLAGDHLRVISSNVKEEALQGLLSKKKIRVERIEKGEPT
                     LEDVFLALAR"
     gene            complement(899..1090)
                     /locus_tag="PLR93_10555"
     CDS             complement(899..1090)
                     /locus_tag="PLR93_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNH27452.1"
                     /translation="MSDIKVLTRSKSNRMIAGVCAGLGDYLNIDPTIVRLLFVLGFFT
                     FHGAMLFVYLIMALVTPEQ"
     gene            complement(1150..1905)
                     /locus_tag="PLR93_10560"
     CDS             complement(1150..1905)
                     /locus_tag="PLR93_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNH27453.1"
                     /translation="MGTWICHLRIAENLLPHLPEIDPITFTFGNLAPDSGIPNEDWTE
                     FDPPKEVTHFLNKGEGEGYIRDLVYFREYLANLEADKNLQKYSFRLGYFTHLICDVLW
                     YYYLDDTMQAGYRELIEKDASKAWGLFKKDWYSLDQQYNQIHKEGLFWKVIFLNPNPP
                     SFLPFVRENALHQQYDYIRKFYSEYDPYWFEERTFPYLNAATMDRLVEDMTQATLFIL
                     ENIKSAKHQDLQSSFQLLPQAMQRPYEMPLGDV"
     gene            complement(1905..>2172)
                     /locus_tag="PLR93_10565"
     CDS             complement(1905..>2172)
                     /locus_tag="PLR93_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27454.1"
                     /translation="DVLACKKRHEDEVHKLEVLSARNLFQSERVGSVYMKNFTFYGLV
                     GLAFVGFGWWQLKWLGLQAVLFIVLGAFLLYASVANYLEGKKYK"
BASE COUNT          585 a          451 c          545 g          591 t
ORIGIN      
        1 ttggcgattt tgtcgcggtg gatcatatca actttgaagt gaacgaaggt gaagtggttg
       61 gttatcttgg acccaatggc tcgggtaaaa ccactaccat tcgcatgttg cttggtttgc
      121 ttgcccctag cgaaggcgaa gcaacagtat tggggtacga tgtccaccgc caaagcgaag
      181 aagtccgcgc acgggttggg tatatgtcgc agaagtttgc gatttatgat gacctaacca
      241 cgctggagaa tttgaccttt tacggtggcg tgtatgggat taccgacaaa gcccgcatca
      301 tggagacgtt ggaactggtc ggtctctctg ggcatgagca tacgctgaca agggatctgt
      361 ccaccggctg gcgtcaacgg ctatccttgg gcattgcgct tgtccacaaa ccgaagttgc
      421 tctttcttga tgaacccacc tctggtgtgg accctactgc ccgccgcgca ttttgggatt
      481 tgatctatca actagctgaa agtggagtga ccattctagt cactacacat tacatggacg
      541 aagccgaata ctgcgaacgt gtaggtgtga tgcgcgatgg caaattgctg gcaatggata
      601 ccccgacgaa tttgaaaaaa tctattatta cgggtgatgt ctgggaggta tttgcacatc
      661 cgctcgaaga tgggctggac gcactaagtg acgtagatgg cattgaacgc gtcgggttgg
      721 ctggcgatca tttgcgggtt atcagctcaa acgtcaaaga agaggcgttg caaggtttat
      781 taagtaagaa gaagattcgg gttgaacgaa tcgaaaaagg agagccaact ctggaggatg
      841 tctttttagc gttggctcgt taaagatgta ggtcacgctt caggcgtgac ctactgtatt
      901 attgttcggg ggttaccagc gccatgatga ggtacacaaa taacatggca ccatggaagg
      961 tgaagaaacc aagtacaaat aacaggcgga cgatggtagg gtcgatattg agatagtcgc
     1021 ctagaccagc acatacacct gcaatcatgc ggttggattt gctccgcgtg agaactttga
     1081 tatcgctcat ttttgctcct ttcgtttgat tacacttgta ttacgcaaag aagcaaaagg
     1141 agttgcagat taaacatcgc ctaagggcat ttcatatgga cgctgcatgg cttgtggtaa
     1201 taactgaaat gaactttgta gatcttgatg ttttgcgctt ttgatgttct ctaaaatgaa
     1261 taatgtagct tgtgtcatat cttctacaag gcgatccatg gtagcagcgt tgagatatgg
     1321 gaaggttctt tcttcaaacc aatacgggtc atattcactg taaaacttgc ggatatagtc
     1381 atattgttgg tgcaaggcat tttctcgtac aaaaggcaaa aaagaaggtg ggttagggtt
     1441 gaggaaaata accttccaga acagaccttc tttgtgtatc tggttgtact gctggtcgag
     1501 gctgtaccaa tcttttttga acaagcccca tgcttttgag gcatcttttt ctatcagttc
     1561 acgatacccc gcttgcatgg tgtcatcgag atagtaatac cagagtacat cgcaaataag
     1621 gtgagtaaaa tagcccagcc gaaaactata tttttgcagg tttttgtccg cttcaagatt
     1681 ggctaggtat tcacgaaaat acacaagatc gcggatatag ccctcacctt cacctttgtt
     1741 gagaaaatgt gttacctctt tcggcgggtc gaattcagtc caatcctcgt tgggaatgcc
     1801 tgagtctggg gcaagattgc caaaagtgaa agtgattggg tcaatttctg gtagatgggg
     1861 taagaggttt tcagcaatgc gaaggtggca aatccaggtt cccattactt atactttttc
     1921 ccttccaaat aattggcaac ggaagcatag agcaggaatg ctcccagcac aataaatagg
     1981 acagcttgca acccaagcca cttgagttgc caccagccaa aaccaacaaa tgccaatcct
     2041 accaatccat agaaggtgaa gtttttcata tacacagaac cgacgcgttc agattgaaac
     2101 aggtttcgag cgctcaaaac ttccagtttg tgaacttcat cttcatgtcg ttttttacag
     2161 gcgagtacat ca
//
LOCUS       DAOQFT010000444         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33885_length_2167_cov_6.413352, whole genome shotgun sequence.
ACCESSION   DAOQFT010000444 DAOQFT010000000
VERSION     DAOQFT010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33885_length_2167_cov_6.413352"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLR93_10570"
     CDS             <1..456
                     /locus_tag="PLR93_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27455.1"
                     /translation="VKTAMQTDDELYYGVCPREVMEARYPNSKRTYTKLKEMELCGAD
                     VNLVRISIIKEHMAVWEALIGNRKSPLRQAGIIGFDTLFQLATRQFTLESLVERASER
                     IGIKGRAIVWDKAEPCMDVDKPHQLELMREDLTRQIRSEKAKARTAEKK"
     gene            453..1013
                     /locus_tag="PLR93_10575"
     CDS             453..1013
                     /locus_tag="PLR93_10575"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH27456.1"
                     /translation="MSLRSILELDARLSNQMRVAEKPGILRNIAIFFAHSGDSWFWAA
                     ALIIIWFFSSSEWRHWETVEFFGLSGLAVVVLAVKFLVKRKRPEGEWGGIYRNTDPHS
                     FPSGHAARAFLIAVLASALAPTWLATILWVWAPLVALARVAMGVHYLSDIIAGAILGI
                     IVALVGLQFYQYLVDWFAGLTGFHFW"
     gene            1062..1460
                     /locus_tag="PLR93_10580"
     CDS             1062..1460
                     /locus_tag="PLR93_10580"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNH27457.1"
                     /translation="MKTHTFRLKPQQDLFDSIEEFVKQNNIEAGCILSSVGSLTHATL
                     RLANREHYNEYEGHFEIVSMTGTVSIHGSHVHVAISDGDGVTIGAHLVSGCKVYTTAE
                     IVLLELDDVVYKRELFENDSGYEELVVYKK"
     gene            complement(1504..2010)
                     /locus_tag="PLR93_10585"
     CDS             complement(1504..2010)
                     /locus_tag="PLR93_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27458.1"
                     /translation="MLILFSVLRFVHIIAGIFWAGGAILMNLIIGPAIGATGDAGKQF
                     VGHLMGKTIFSKFMLASGAITVLAGSILYGIDSNWFSSGWMMSPTGTGFGIGAISGII
                     ALVFGFLIGSTNGKLALLGMQIQGKPTNEQLAALGALRKRQVIVTNGNTIFILISIAL
                     MASARLFG"
BASE COUNT          597 a          548 c          444 g          578 t
ORIGIN      
        1 gtcaaaaccg ccatgcaaac cgatgatgaa ctctactacg gtgtttgtcc gcgtgaagtg
       61 atggaagccc gctaccctaa ctccaaacgc acctacacca aattaaaaga gatggaactt
      121 tgtggcgcag atgtcaacct cgtccgtatc agtatcatca aagaacacat ggcagtttgg
      181 gaagccctca tcggcaaccg taagagtccc ttgcggcagg ctggcattat tggctttgac
      241 accctctttc aacttgctac ccgccaattc acccttgaaa gtttggtcga acgtgcatca
      301 gaacgcatcg gcattaaagg gcgagccatc gtatgggata aagctgaacc ctgcatggat
      361 gtggataaac cccatcagtt ggaattgatg cgcgaagacc ttacacgtca gatccgttct
      421 gaaaaagcca aagcaaggac ggcagagaaa aaatgagtct gcggtctatc cttgaactcg
      481 atgcccgact ttccaatcaa atgcgcgttg cggaaaagcc gggcattttg cgcaacatcg
      541 ccatcttctt tgcccactcc ggcgactcat ggttttgggc agcagccctc attatcatct
      601 ggttctttag cagctccgaa tggcggcact gggagaccgt agaatttttt ggtctttccg
      661 ggttagcagt tgtcgtgctg gcggttaaat ttctcgtcaa acgcaaacgc cctgaaggcg
      721 aatggggagg catttaccgc aatacggatc cgcactcctt cccatcagga cacgccgccc
      781 gcgccttcct catcgcagtc cttgcctcgg ctcttgctcc gacctggctt gctactatcc
      841 tttgggtttg ggcgccgctg gtagctttag ctcgcgtagc catgggcgtg cattatctct
      901 ccgatatcat tgctggagcc attctcggca tcatcgttgc cttggtgggc ttgcagttct
      961 accaatatct agtcgactgg tttgccgggc tgacaggctt ccatttctgg taatcttttc
     1021 gtgcgagagt taaactctcg cacgaacttt ttataaaacc tatgaaaaca catacctttc
     1081 gtcttaaacc tcaacaagac ctctttgact ccattgaaga gttcgtaaaa caaaacaata
     1141 tcgaagccgg ctgtatcctt tcgagtgtag gtagcctaac ccatgctact ttgcgccttg
     1201 caaaccgcga acattacaac gagtacgaag ggcattttga aattgtctcc atgaccggca
     1261 ccgtatcgat tcatggttca cacgttcatg tcgctatctc tgatggagat ggcgttacca
     1321 ttggtgctca cctcgtaagc ggctgtaagg tctacaccac cgcagagatt gtgttactcg
     1381 aattagatga tgtagtttat aagcgcgaat tattcgaaaa tgactcgggc tacgaagaac
     1441 tggtcgtgta taaaaaataa aaacagacac ccttgatggg tgtctgtttt tattgaaacg
     1501 catttatcca aataatcgtg cagatgccat caaagcaatc gatataagaa tgaaaattgt
     1561 gtttccgttt gttacaataa cctggcgttt gcgcaatgca cctaaagccg ctaattgctc
     1621 attcgtaggt ttaccttgaa tctgcatgcc aagtaaagct aatttcccat ttgtacttcc
     1681 gattaagaac ccaaagacca acgctataat cccagaaata gcaccgattc cgaagccagt
     1741 gcctgtcggg ctcatcatcc atccagagct aaaccaattt gaatcaatac cgtatagaat
     1801 acttccagcc aaaacggtta ttgctcccga agcaagcata aatttcgaaa atatagtttt
     1861 tcccattaag tgaccaacaa attgtttgcc cgcatctcca gttgcgccaa tggcaggtcc
     1921 aataattaag ttcatcaaaa tagcgccacc tgcccagaaa attcctgcaa taatatgtac
     1981 aaatcgtaaa acagaaaaca aaattaacat ggtactctcc tttaaaattt atcaaatcta
     2041 ataataagat atattttatc cattttaatt cacaaaacaa aatccttcta aagtcttgct
     2101 ccttcatgta gaataacccc atgcccgatt tgtccacctc cttacacaaa caagatttgg
     2161 gacactt
//
LOCUS       DAOQFT010000445         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_33904_length_2166_cov_8.860256, whole genome shotgun sequence.
ACCESSION   DAOQFT010000445 DAOQFT010000000
VERSION     DAOQFT010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33904_length_2166_cov_8.860256"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLR93_10590"
     CDS             <1..709
                     /locus_tag="PLR93_10590"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH27459.1"
                     /translation="ATRGGVLMQIVLAILAVVLAYVIGSVPVGLLIVKIVTGKDIREV
                     ESGRTGGTNAMRAAGFWAGFATAMLDIVKGAAGVWVARWLTPDLPVVHMIAPLAAILG
                     HNHSIFLPERDENGRIIRLRGGAGGAPSVGGAMGLYLPSILIVLPLGMLTFFSIGIAS
                     VTTMAVALYSTLAFAYYASQGIIPWAYVLYGVGAEILLVLALRPNIKRLFEGNERVVK
                     YSLNGWLRKRKEEKAAK"
     gene            809..1387
                     /locus_tag="PLR93_10595"
     CDS             809..1387
                     /locus_tag="PLR93_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH27460.1"
                     /translation="MSTREKILDTALTLFNNEGTAAVSTNHIAEAASISPGNLYYHFH
                     NKEEIIRGLFERLFVANDAAFRLSEDKLPTLNDLQTMVKTNYKILWQYRFAHRELAAL
                     LRADEELRTRFLSVRKRGFEGFGQLFQAFTQAGVFKTQSTPEEIQNLAEVCWLITEFW
                     LNSLEVSGKTVNEAQMSHGVELMMQVLKPFIK"
     gene            1397..2104
                     /locus_tag="PLR93_10600"
     CDS             1397..2104
                     /locus_tag="PLR93_10600"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNH27461.1"
                     /translation="MSQNNFRKLAGLFFIVGAVLVNIPYTLLIMNFDYPDILRLPTAE
                     ILTRFQAGGNALIYTWLAFAWVGLPMLFGAIMLKRILADEQAPFLETATTLGVIGFIV
                     QVVGLLRWVFVVPALARLYTDPATDSATQAAVAAVFTAVHQYGGVILGEHMGQFLIIL
                     WMSMISGIMYKSKMFSKWVAILGWVASAIYLLAQTELLATAIPTFPVIDWAGLVGSLL
                     WLVWMIVMGVYLVKYKE"
BASE COUNT          520 a          527 c          533 g          586 t
ORIGIN      
        1 agcaactcgt ggaggagtct taatgcaaat cgtcttagcc attctggcag ttgtactggc
       61 ttatgtaatc gggtcggttc cggtggggtt actgatcgta aagatcgtga ccggaaaaga
      121 tattcgcgaa gtggaaagcg ggcgcaccgg cggtacgaac gccatgcgtg cggcagggtt
      181 ttgggctggg ttcgccactg ccatgctcga cattgtgaaa ggcgcagcag gggtttgggt
      241 ggcacgttgg ctcacccccg atctgcccgt tgttcacatg atcgctccct tggcggcaat
      301 tctcggacat aaccattcga tctttctgcc cgaacgcgat gagaacggac ggatcattcg
      361 tttacgtggc ggtgcaggcg gcgctccatc tgtaggtggc gcaatgggct tgtatcttcc
      421 atctattttg atcgtcctgc cgcttggcat gttgaccttc tttagtattg ggattgcttc
      481 tgttacaacc atggcggtag cgttgtattc cactttggca tttgcttatt atgcttcaca
      541 aggcatcatc ccttgggcat atgtccttta tggggtagga gcagagattc tgttggtgtt
      601 ggcattacgc ccgaatatca aacgtctctt tgagggcaat gaacgggttg taaaatacag
      661 cctcaacggc tggctgagaa aacgtaaaga agaaaaagca gcgaagtaag ctaaaacgcc
      721 gaggtcttga agacctcggc gtttttttac ccttgacaga ttagagtatt tactctaaaa
      781 tattagagta aatactctag gagacattat gtccactcgt gaaaagattt tagataccgc
      841 cctcaccctc ttcaataacg aagggactgc tgccgtcagc accaaccaca tcgccgaggc
      901 ggcaagcatc agtccgggga atctctacta tcacttccac aataaggaag agattattcg
      961 aggtctattc gagcgtctat tcgtagcgaa tgatgcggca tttcgtttgt cggaagataa
     1021 attgcccacg cttaatgacc tgcaaacgat ggtcaagacc aattacaaaa ttctctggca
     1081 atatcgtttt gcacaccgcg aacttgccgc actcttgcga gcagatgaag aactacgcac
     1141 gcgctttctc tctgttcgta aacgtgggtt tgaagggttt ggtcagctct ttcaagcctt
     1201 tacccaagct ggcgttttca aaacacagtc aaccccagaa gaaattcaaa accttgccga
     1261 ggtctgctgg ctcattactg aattttggct gaacagcctt gaagtaagcg gcaagaccgt
     1321 taacgaagca caaatgtctc atggcgttga gctaatgatg caagtattaa aaccatttat
     1381 caaataggaa aaaatcatgt cacaaaataa ttttagaaaa ctagccggtc tcttttttat
     1441 cgtgggtgca gtgttggtca atattcccta cacgctgctc attatgaatt tcgattaccc
     1501 cgatattctg cgcttaccca cagcagaaat tctcacacgg tttcaagcag gcgggaatgc
     1561 cctcatctac acttggcttg ctttcgcatg ggtgggttta ccaatgctct ttggcgcgat
     1621 catgctcaaa cgaatcttag ctgatgaaca agctccattt ctagaaacgg cgacgacact
     1681 cggtgtgatc ggcttcatcg tccaggtggt gggactcttg cgctgggtct tcgtcgtgcc
     1741 tgccctcgcc cgcctctaca cggacccggc aacggattca gctacccaag ccgcagtcgc
     1801 cgctgtattc actgccgtcc accaatatgg cggagtcatc ctcggcgaac atatgggaca
     1861 attccttatc atcctgtgga tgtctatgat cagcggcatt atgtacaaat ccaagatgtt
     1921 tagcaaatgg gttgccattc tgggatgggt tgcctctgcc atttacctcc tcgcgcagac
     1981 ggaacttctc gccaccgcta ttcccacttt ccccgttatc gattgggcgg gactggttgg
     2041 cagcttactt tggctggtct ggatgatcgt gatgggagta tatttggtga aatataagga
     2101 atagtatgca attccctgaa ccctttctct caaacatcaa acattctttt ggcgaggctg
     2161 gcgaga
//
LOCUS       DAOQFT010000446         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_34072_length_2160_cov_4.788124, whole genome shotgun sequence.
ACCESSION   DAOQFT010000446 DAOQFT010000000
VERSION     DAOQFT010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34072_length_2160_cov_4.788124"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..1215)
                     /locus_tag="PLR93_10605"
     CDS             complement(361..1215)
                     /locus_tag="PLR93_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNH27462.1"
                     /translation="MTITKTFRRALRPYGKTAQAIWRWKRFSFNDAPPIFANSKPKSG
                     SHLLLQVMNGLTQIMPYKYVDADPVRTITYEGRRKTKEEILADLKHTPTGVIGWGYVD
                     ATEENASFLTSDGRVNFFIYRDPRDMLVSQVFFATDMHEEHGMHDYYNSLPDFGARLN
                     VAITGIDKDDLRMVSVKQRYEGVFAWLEQKNTMCIRFEDLINNRDATLLKMLDEVEST
                     GYKIPTPREKALAVLVDAIQPRKSHTFRSGKTGGWRQHFTDDHKKLFKDVAGDLLVRL
                     GYEKDNDW"
     gene            complement(1272..>2160)
                     /locus_tag="PLR93_10610"
     CDS             complement(1272..>2160)
                     /locus_tag="PLR93_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNH27463.1"
                     /translation="EKMAREVKFGNRMMGDGHPAYVIAEIGINHNGDLDIAKKIIDAA
                     VHAGADAVKFQKRTPEVCTPLDQQKQMRETPWGYITYLDYRYKVEFSEEQYREIDRYC
                     KEKKIDWMVSVWDEPSVDFMEKFETPAYKVPSASLTDHNLLKYVRKTGKPVIISTGMS
                     TMEQIHKGVNAVGEENLVIMHCTSTYPCEPEELNLRMIETLRKEFPNNPIGYSGHETG
                     LVPSAVAIALGATSIERHITLDRAMWGSDQAASVEPHGFASLVKYIRVTESALGDGVK
                     RIYDSEKGPMKKLRRVVNE"
BASE COUNT          522 a          551 c          505 g          582 t
ORIGIN      
        1 acgctatatt gaatgaaatc ctgtatctgg gattcaagag taatttcgaa cggtgaacga
       61 gcttcgccca ttccacgagg cggaggttga ttcgtgatca ggactgtgat aaatacaccg
      121 ataaaaacag ccagccagat atttgccaat tttcccaata ggtaacgcaa agcgcttccc
      181 caacctcgca agcggtgaga tatccctgct ggaatgttac ttttttccgt cacaagaatg
      241 ttatacaggg gacgatatgt tcttcccacc ttccagggaa ggataaggaa accgttctag
      301 atgcgggtat ttggaaacat ctggtaacta aaaggatgga tttgcatcca tcctctgtca
      361 ctaccagtca ttatcttttt cgtaccccag ccttactaac agatctcccg caacgtcttt
      421 aaataatttc ttgtgatcat cggtgaaatg ctgtctccaa ccgcccgtct tgcctgaacg
      481 gaaggtgtga ctctttctcg gctgaatggc atctacaaga actgccaatg ccttctcgcg
      541 cggagtgggg atcttgtagc cggtactttc cacttcatca agcattttta gcagagtcgc
      601 atcgcggtta ttgataaggt cttcaaagcg gatgcacatg gtgttcttct gttccagcca
      661 ggcaaacaca ccttcatatc gttgcttcac gctgaccatt ctcaagtcgt ctttgtcgat
      721 gccggtgatg gcgacattaa gtcgcgcgcc aaaatccggc aaagagttgt agtagtcgtg
      781 catgccgtgc tcttcgtgca tatcggtcgc gaagaacact tgtgagacga gcatatctct
      841 cgggtcgcga taaatgaaaa agttgacgcg cccatccgag gtcaggaaag aggcgttttc
      901 ttctgtcgca tccacatagc cccagccgat cactcccgtt ggggtatgct tcaagtccgc
      961 caggatctct tctttcgtct ttcttcttcc ttcatatgtg attgtccgca cggggtcggc
     1021 atccacgtac ttgtaaggca tgatctgggt caacccgttc atcacctgca aaagcaaatg
     1081 tgagccgctc ttgggtttgg agttggcaaa gatgggcggt gcgtcgttaa acgaaaaccg
     1141 cttccatcgc caaatggctt gggcggtttt gccatacggg cggagtgcgc ggcggaaagt
     1201 tttggtgatc gtcattagaa attacgcatt acgcattacg aatcacgtga ttcgtaatgc
     1261 gtaaacatca actactcatt caccacacgg cgaagttttt tcattgggcc cttttcagaa
     1321 tcgtaaatgc gtttcacgcc atcgcctaaa gccgattcgg tcacacgaat gtattttaca
     1381 agtgaggcaa agccatgcgg ctccaccgag gcagcctgat cgctgcccca catggcgcgg
     1441 tcaagcgtga tgtgacgttc gatggatgta gcgccgagcg cgatcgccac agccgagggg
     1501 accaacccgg tctcatgccc ggaatagcca ataggattat tgggaaattc ctttcgcagg
     1561 gtctcgatca ttcgaagatt caattcttcc ggctcgcagg gataggtgct ggtgcagtgc
     1621 ataatgacga gattctcttc acccacggca ttcacccctt tgtgaatttg ttccatggtg
     1681 gacatacccg tggaaataat aactggctta cccgttttac ggacatattt cagcaagttg
     1741 tgatcggtca aagaagccga cggaacttta taggcagggg tttcaaattt ctccatgaaa
     1801 tccaccgagg gctcatccca taccgagacc atccaatcga tcttcttttc cttgcaataa
     1861 cgatcgatct cgcgatattg ttcttcgcta aattcgacct tgtaacgata atcaagatat
     1921 gtgatatagc cccacggcgt ctcacgcatc tgcttctgct gatcaagcgg agtacaaact
     1981 tcaggtgtac gcttttggaa tttaaccgca tcggcaccgg catggacagc agcatcgatg
     2041 atcttcttcg caatatccag gtctccgtta tggttgatac cgatctccgc gatcacatat
     2101 gcaggatggc catcgcccat cattcgattt ccaaatttaa cttcacgagc cattttttct
//
LOCUS       DAOQFT010000447         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_34158_length_2157_cov_5.499524, whole genome shotgun sequence.
ACCESSION   DAOQFT010000447 DAOQFT010000000
VERSION     DAOQFT010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34158_length_2157_cov_5.499524"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PLR93_10615"
     CDS             <1..1112
                     /locus_tag="PLR93_10615"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNH27464.1"
                     /translation="EELLNGLQTNFDDIREAGVKAIIRFAYNQGPYPDTEPDASKAQI
                     LRHIEQLTPLLQTNTDVIAWVEAGFIGAWGEWHTSTNGLDNIQDKQDILNAVLAAIPN
                     RFVQVRYPANIIEMYPSPEDAVNARVAHHNDCFLSSETDVGTYERNGEITIERDQAYL
                     AELTRSTPMSGETCAPFPPRSECTNAIQEMKLLHFSAINEAYHKGIIRSWEDGGCLQE
                     IADRMGYRLALTSADFNDQVRPGGILNLSVTLENKGFASIINERFLYIVLFNGSERYE
                     VKTSIDPRAWQPGTSSFDIQLHVPSNVDEGEYQLALWLPDAYETLHDNPLYAIQFANE
                     NTFDEATGYNILGIIKVDKNTSGNYQHGEEFEVIK"
     gene            1109..1651
                     /locus_tag="PLR93_10620"
     CDS             1109..1651
                     /locus_tag="PLR93_10620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH27465.1"
                     /translation="MTELQVRFVPKAEITSELDSEIDVLDKLAFADDGIDHDPEFSSI
                     HWAEPDWMALGFLGEELVTQLCLPKREISVGAEKVWVAGIGGMATHPKHHHKGYGSKL
                     LIATETFMRNEMCVPFGLLICADETRPFYELARWQVAANWLAYHQDNERKILRTCVMI
                     LQLQDQTWLAGEIDLCGAPW"
     gene            1713..>2157
                     /locus_tag="PLR93_10625"
     CDS             1713..>2157
                     /locus_tag="PLR93_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559307.1"
                     /note="catalyses the hydrolysis of terminal non-reducing
                     alpha-D-galactose residues in alpha-D-galactosides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNH27466.1"
                     /translation="MPKITFIGAGSTVFAKNLLADILAFPELANSTISLMDIDPVRLK
                     TTEIVAHKIAEFFGAKPKIEATLDRRAALDGADYAISMFQVGGYKPSTVIDFEIPKKY
                     GIKQTIADTLGIGGIMRGLRTIPVFLDICKDMEEVCPDVTFLQYVN"
BASE COUNT          578 a          596 c          490 g          493 t
ORIGIN      
        1 ctgaggaatt gctgaacgga ctacagacca acttcgacga tatccgcgag gcgggcgtca
       61 aagccatcat ccgctttgcc tacaatcagg gaccatatcc cgataccgaa cccgatgcgt
      121 ccaaagcgca gatcctgcgc cacatcgagc agttgactcc gctcctgcaa accaatacgg
      181 atgtcattgc ctgggttgaa gcgggtttca tcggcgcctg gggcgaatgg cacacatcga
      241 ccaatggttt ggataacatt caagacaaac aggatattct caacgctgtc cttgccgcca
      301 ttccaaatcg ttttgtgcaa gtgcgctacc ctgccaatat catcgagatg taccccagcc
      361 cagaagacgc ggtcaatgca cgagtggcgc atcacaacga ttgttttctt tccagcgaaa
      421 ccgatgttgg cacctacgaa cgcaatggcg agatcaccat cgaacgcgac caagcctacc
      481 ttgccgagtt gactcgctcc acgcctatga gcggcgagac ttgcgccccc ttcccaccgc
      541 gttcagaatg cacgaatgcc attcaagaaa tgaaactact gcatttctcc gccatcaatg
      601 aagcgtatca caaaggcatc atccgttcgt gggaagatgg cggctgcttg caggagatcg
      661 cagatcgcat gggctaccgc ctcgcattga cctcagcaga tttcaatgat caagtgcgtc
      721 caggcggcat tctcaatctc tctgtaactt tagagaacaa aggatttgct tcgatcatca
      781 atgaaaggtt tttatatatt gttctcttca acggctccga acgctatgaa gtcaaaacct
      841 ccatagaccc gcgtgcatgg cagcctggaa cttcatcgtt cgatattcaa ctgcatgtcc
      901 cctccaatgt agacgaaggc gaataccaac tcgcgctctg gcttcctgat gcctatgaaa
      961 ccttgcatga caatccgctc tacgccattc aattcgccaa cgaaaatacc ttcgatgaag
     1021 ccacaggcta caacattcta ggcatcatta aagtggacaa aaatacaagc ggcaattatc
     1081 aacatggaga agaatttgag gttatcaaat gaccgagttg caagtccgct ttgttcccaa
     1141 agcagaaatc acatcagaac ttgacagtga aatcgacgtc ctggataaac tcgcttttgc
     1201 cgacgatggc attgaccatg atcctgagtt ttccagcatc cattgggcag agcctgattg
     1261 gatggcactt ggttttctag gtgaagaatt agttactcaa ttgtgccttc ccaagcgaga
     1321 gatttccgtc ggcgctgaaa aagtttgggt ggcaggcatc ggcggtatgg cgacgcatcc
     1381 aaagcaccac cataagggat atggctcaaa gttgcttata gcgacagaaa cttttatgcg
     1441 aaatgagatg tgtgtaccat ttggtttgct catctgtgca gacgagacgc gtccgtttta
     1501 tgaacttgct cgctggcaag tcgccgcaaa ctggctcgcc tatcatcaag acaatgaacg
     1561 aaagatattg cgcacgtgtg taatgatctt gcagttgcaa gatcaaactt ggcttgcagg
     1621 tgaaattgac ctgtgtggcg caccgtggta atcaaatgcc atcgtagatc aaacctgaaa
     1681 cataaacact taatactaac taggagaacc ccatgccaaa aatcactttc atcggcgcag
     1741 gcagcaccgt cttcgccaag aacctgctcg cagacattct cgccttcccc gagcttgcca
     1801 attccaccat cagcttgatg gacattgacc ctgtgcgcct caaaaccacc gagatcgttg
     1861 cacacaaaat cgcggagttc ttcggcgcaa aaccgaagat cgaagccacg ctcgatcgtc
     1921 gcgccgctct cgatggcgca gactatgcca tcagcatgtt tcaggttggc ggctacaaac
     1981 ccagcaccgt catcgacttt gagattccca agaaatacgg catcaaacaa accatcgccg
     2041 atacgctcgg catcggcggc atcatgcgcg gactacgcac catccccgtc tttctcgata
     2101 tttgcaaaga catggaagaa gtctgccccg atgtgacctt cctgcaatac gtcaacc
//
LOCUS       DAOQFT010000448         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_34265_length_2153_cov_3.864156, whole genome shotgun sequence.
ACCESSION   DAOQFT010000448 DAOQFT010000000
VERSION     DAOQFT010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34265_length_2153_cov_3.864156"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLR93_10630"
     CDS             <1..829
                     /locus_tag="PLR93_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27467.1"
                     /translation="HAATNVPSQLVTNEIQKLSTVKPDVAASLHQLVEEGSRTNPAIL
                     IPMDASLKTPRDRVYPMNVEVPLADLNLLAMHAQTLNILEAYESASGGDRAVIFASFN
                     KFQKAYLSTIAGFGAYLTRAKEMAAQGENTAAGAIKLLAHLPLPIQRLLDKIPERFEV
                     LNNLIKGREVFSNVGAVVPSSTLTRFMTAKDDNAQKQLAWGIITDAKSVLRIHLRDFR
                     PHVQALSSIGRKDLASLITQDYLNAYAEGFNQYIRDLSRITTASRETMTKPQAKGNS"
     gene            832..>2153
                     /locus_tag="PLR93_10635"
     CDS             832..>2153
                     /locus_tag="PLR93_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH27468.1"
                     /translation="MKDPMIGMQLANFKVESLLGQGGMAMVYFGQDIKLHRPVAIKVL
                     DKRYKNHPAYAGRFVNEARMMAKWHHENIIQIYYADDTQGFPYYVMEYVDGQDLGSVI
                     DAYQADGNFMPHDEVLRIGKAIASALDYAHRHGVVHRDVKPSNILLSKDGRVLLGDFG
                     MALEVRDGSMGNIFGTPHYISPEQAKRSADAVPQSDIYSLGVIMYEVLAGAAPFNDSS
                     PANIALQHISQKPPAPSTINPSLSRAVESVLLKVLEKNVKDRYQTAMKFMDALEDALS
                     ASRVSEKASLPPLPVGVPTIHRNEYQPEQHSRKKVIENQAPVAKMPATVRANAPMPKR
                     KTWLWVVLVLLLLGFGGFYFLRNGFNLPALPSQSTATTESAAPLSTPQPVLPTDTPVP
                     SPTFTATETIVPPTATIEPSPTIVLTPTVKYPEGANYTLFYNETSLFI"
BASE COUNT          557 a          553 c          485 g          558 t
ORIGIN      
        1 tcacgcggcg acgaatgtcc cttcgcagtt ggtcacgaat gaaattcaaa aactttccac
       61 cgtcaagcca gatgttgccg catccttaca tcaattggtg gaggaaggca gccgtacaaa
      121 tcctgctatc ctcatcccta tggatgccag cctgaaaacc ccgcgtgatc gggtgtatcc
      181 tatgaatgta gaagttccgc ttgcggattt aaatctgctc gcgatgcatg ctcaaacgtt
      241 gaacatactg gaagcgtatg aatccgcttc gggcggagac cgagccgtga tcttcgccag
      301 tttcaataaa ttccagaagg catatctctc cacgatcgct gggtttggcg cttacttgac
      361 ccgcgccaaa gaaatggcag cacaaggcga aaacacagcg gctggggcga tcaaattgtt
      421 agcacatttg cctctgccta ttcaaaggct tttagataag attccggagc gctttgaggt
      481 actcaataac ctcatcaaag ggcgcgaagt attttcgaat gtaggcgctg tggtcccctc
      541 aagtacgctt acgcgtttta tgacagcaaa agatgacaac gcccaaaaac agctcgcttg
      601 gggtatcatc accgatgcga aatctgtttt gcgaattcat ctgcgcgatt ttcgtccgca
      661 tgttcaggct ttgtcatcca ttggacgcaa agaccttgct agcctcatta cgcaagatta
      721 tttgaatgcc tatgctgaag gattcaatca atacatccgc gatctttcgc gtattacaac
      781 tgccagtcga gaaactatga ctaagcccca agcaaaagga aattcctagc catgaaagat
      841 ccgatgatcg gaatgcagct cgccaacttc aaggttgaga gcctattagg tcagggcggt
      901 atggcgatgg tctattttgg gcaggatatt aaactgcatc gaccagttgc tattaaggtt
      961 cttgataagc gctacaaaaa ccaccctgcc tatgccgggc gtttcgtcaa cgaagcacgg
     1021 atgatggcaa agtggcacca cgagaatatc attcagattt attatgccga tgatacacaa
     1081 ggctttccat attacgttat ggaatatgta gatggtcaag accttggctc tgtcattgat
     1141 gcatatcaag cagacggaaa tttcatgccg catgatgaag tgctgcggat tgggaaagcg
     1201 attgccagtg cattagatta tgcccatcgg catggggtcg tgcatcgcga tgtaaagcct
     1261 tcaaatattc tcctctccaa agatgggcgt gtattgcttg gtgattttgg tatggcgctt
     1321 gaagtgcgtg atgggtccat gggaaatatt ttcgggacgc ctcattacat ctcgcccgaa
     1381 caagccaaac gttcggcaga tgcggtccct caatctgata tttattcctt gggcgtcatc
     1441 atgtacgaag tccttgccgg agcagcacca ttcaatgact cttccccagc aaatattgct
     1501 ttgcaacata tttctcaaaa gccgcctgct cctagcacga taaacccgag cctgtcgcgt
     1561 gccgttgagt ctgttttgct gaaggtactt gagaagaacg tcaaagatcg ttatcagacc
     1621 gccatgaagt ttatggacgc cttggaagat gcgctgtcgg catcacgtgt ctcagaaaaa
     1681 gcttctttgc ctccgctccc tgtaggtgtt cctacaattc atcgtaacga atatcaacct
     1741 gaacagcact ctcgaaagaa agtaatagaa aatcaagcac ctgttgcaaa gatgcccgct
     1801 actgttcgcg caaatgcccc catgcctaag cgcaaaactt ggctttgggt cgtcttggtt
     1861 ttactacttc ttggattcgg cggtttctac ttccttcgaa acggattcaa cctgccagcg
     1921 ttgccttctc aatcgacagc tacaacagaa tccgccgcgc ctctttctac gcctcaacct
     1981 gttctgccaa ctgatacacc cgtcccctcc cctaccttta ccgcaacaga aacgattgtt
     2041 cctcccaccg ctacgatcga accatcgcca acaattgttc taacgcctac agtgaagtac
     2101 ccagaaggcg caaactacac cctgttttat aacgagacca gtttgtttat tat
//
LOCUS       DAOQFT010000449         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_34492_length_2144_cov_4.953566, whole genome shotgun sequence.
ACCESSION   DAOQFT010000449 DAOQFT010000000
VERSION     DAOQFT010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34492_length_2144_cov_4.953566"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="PLR93_10640"
     CDS             <1..114
                     /locus_tag="PLR93_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH27469.1"
                     /translation="RLRWKFETGGPITGSPVVLDDIVYFGSTDHHVYALFA"
     gene            126..1037
                     /locus_tag="PLR93_10645"
     CDS             126..1037
                     /locus_tag="PLR93_10645"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNH27470.1"
                     /translation="MADFFKKLFGSKEESTKPRQVDSATTAPLSDQQLQSILSSQAPR
                     YEIKQLVAAVGQSVGKQRDHNEDSVLSLTNTISGSSESVPFGLYIVADGMGGHQFGEV
                     ASNAAIRIVGGNITKKFHSYLYKLPTQSLQESLQEVMESSVMEAHQYIQREAPGSGTT
                     ITAALVLGQQVTIGHVGDSRAYAVYPDGRMDPLTRDHSLVKRLEELGHLSKDEVENFP
                     HRNVLIRALGQGEVLEADVFTVPFPQSGYLMICSDGLWGVVNERDVARIINEAPNLQR
                     ACQSMVEAANAAGGPDNITVILAQLIG"
     gene            1046..>2144
                     /locus_tag="PLR93_10650"
     CDS             1046..>2144
                     /locus_tag="PLR93_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNH27471.1"
                     /translation="MSASKPDLYSVLGVLRDATQEEIKHAYLAAAQRLHPDKNVAAGE
                     TELFLDIQQAYEVLSNPKRRSQYDSTLPPEVEEKNQFIRHEVLFSRPSLVHLKEDQLL
                     YVLLEVEPTQKQSASLPTPPLNLCLVLDRSTSMQGDKMDILKSTAIQLIRSLRPQDVL
                     SIVTFSDRAEVIVPASISLDKRKQEGNVQMIRPSGGTEIFQGLDAGFKEVRRTLDPAR
                     VNHIILLTDGNTYGDEQDCLQLAEQAAALNIGITGMGIGHEWNDIFLDALAAKTGGSS
                     VYISQPKDIERFLIEKFKALTSVYADEVSLDIRELEHIHLNFAFRIQPEGSQIATTSP
                     LRMGPILRDVPLHVLLEFVVSPEALKGEKVLV"
BASE COUNT          562 a          490 c          507 g          585 t
ORIGIN      
        1 cgtttacgtt ggaaatttga aacaggtgga cccatcaccg gttcccccgt tgttttagat
       61 gatattgttt attttggctc aaccgaccat cacgtttatg cgttgtttgc ctaaaaggag
      121 aacttgtggc tgattttttc aagaaacttt ttggttccaa agaggaatca acaaaaccaa
      181 gacaagtgga ttctgctaca acagctccgc tttctgacca acaactccag tccattttat
      241 ccagccaggc acctcgctat gagatcaagc aacttgttgc ggctgtaggt cagtctgtag
      301 ggaaacaacg tgaccacaat gaggatagtg tcctttccct gacgaacacc atttcaggta
      361 gttctgagag tgtaccattt ggcttatata tcgttgccga tggtatgggt ggacatcaat
      421 ttggtgaagt agcaagcaat gctgctattc gcattgtcgg tggcaatatc actaagaaat
      481 ttcattctta cttgtataaa cttccaactc aatcattgca ggaatccttg caagaagtga
      541 tggagtcctc tgtgatggaa gctcatcagt atattcaacg ggaagccccc ggtagtggta
      601 ctaccatcac agctgccctt gtacttggtc agcaagttac gattgggcat gtgggggata
      661 gccgtgcata tgcggtttac ccagatggca gaatggatcc attgacccgt gaccactctt
      721 tggtcaaacg tcttgaagaa ctcgggcatt tgagcaaaga tgaagtggaa aactttccgc
      781 atcggaatgt tttgatccgt gccttgggtc agggtgaagt acttgaagca gatgtcttta
      841 cggttccttt cccgcaatcg ggatatctta tgatttgcag cgatggactt tggggtgttg
      901 taaacgagag agacgttgcc cgtattatta atgaagcccc aaatttgcaa cgggcatgtc
      961 agagcatggt agaagctgcc aatgcggctg gaggaccgga taatatcacg gtcattctcg
     1021 ctcaacttat tggataaccc ctctcatgtc tgccagcaag ccggatctat actccgttct
     1081 aggtgtgtta cgtgatgcca cacaggagga gattaagcat gcctatttgg ctgccgctca
     1141 acggctgcat ccagataaaa acgtagcggc tggcgagact gaactcttcc ttgatattca
     1201 acaggcgtat gaagttctct cgaaccccaa acgccgcagt caatacgact ctacgctccc
     1261 acctgaagtt gaagaaaaaa atcaatttat tcgtcacgag gtccttttca gccgtcctag
     1321 tctggttcac ttaaaagagg accaattatt gtatgtgctg ttggaagttg aacctacaca
     1381 aaaacaatct gccagcctac ctacgcctcc attaaacctt tgcttggtgt tagaccgatc
     1441 tacctccatg caaggcgata agatggatat cttgaagtct acagcaattc agctaatccg
     1501 tagtttaaga ccacaggatg tgctaagcat cgtcaccttt agtgaccgtg ctgaagttat
     1561 cgtccctgct tcgatcagcc tagacaaacg caaacaggaa ggcaatgtgc aaatgatccg
     1621 tccttctggt gggacggaaa tctttcaggg attagatgcc gggttcaaag aagttcgccg
     1681 aactctagac ccagcacgtg tcaatcacat tattcttctt accgatggaa acacctacgg
     1741 cgatgagcaa gattgcttgc aacttgcaga gcaagctgct gccctgaata ttggtattac
     1801 cggcatgggg attgggcatg aatggaatga catcttttta gatgctcttg ctgcaaaaac
     1861 cggcggatct tccgtttata tatcccagcc gaaggatatt gaacgcttcc taatcgaaaa
     1921 attcaaagcg ttgaccagtg tttacgctga tgaagtgtcg ctggatatca gagaacttga
     1981 gcacattcac cttaactttg cgtttcgcat ccagccggag ggcagccaaa ttgcaaccac
     2041 ttcgcctttg cgaatgggac caatcttgcg tgatgtgcct ttgcatgtct tgttggaatt
     2101 tgttgtcagc cccgaggcat tgaaagggga gaaggtctta gtat
//
LOCUS       DAOQFT010000450         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_34600_length_2140_cov_6.241727, whole genome shotgun sequence.
ACCESSION   DAOQFT010000450 DAOQFT010000000
VERSION     DAOQFT010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34600_length_2140_cov_6.241727"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLR93_10655"
     CDS             <1..324
                     /locus_tag="PLR93_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27472.1"
                     /translation="CRFYEREMTAYSIARGIEYIYEECPFAEGATQIFYKETLNQLEN
                     TRPGIKLTFYLRFLEARQSGELFIQREETQPHIHPCEKCGQPTSAPGLCSFCRMLEKA
                     DVVTE"
     gene            complement(331..1320)
                     /locus_tag="PLR93_10660"
     CDS             complement(331..1320)
                     /locus_tag="PLR93_10660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH27473.1"
                     /translation="MPQSLLNGEILRERYVVREQIGQGGTGNIYLADDVRLQGRLCAI
                     KEVEHNQVLPSDVLAQAREQFFREASVLARLDHPNLPKVSDFFSEETRDYLVMDYVPG
                     KDLRQLMQEARRSNTFIPEREVLSWANQIAEALNYLHHQDPPIVHRDIKPSNIKLTPS
                     GLVKLVDFGLVKIMAPGEVTITVIQGQGTALYTPLEQYGGDDTHTDAQADIFSFGATL
                     YHLLTNEPPAEARKRFLNSRSLTPPIDINPRISSHVEKAIFWAMALHPNDRPVDVLTF
                     RDALFGKIEVPIQVDIERYGFETPRFSVFSTEQIAGYVAFGLFLVGLLATVIR"
     gene            1509..>2140
                     /locus_tag="PLR93_10665"
     CDS             1509..>2140
                     /locus_tag="PLR93_10665"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNH27474.1"
                     /translation="MEREEEYPILVAQDGPLKGQRWTLSKTLMMGRDPSCDIQVQDRQ
                     VSRFHARITPTQEGVTLEDLGSKNGTNHNGTEIAGPIMLQDGDAFGIALAQQFTFLTS
                     DATMPLAESGPRSGRLVMEQKSRQVWVNQQQLIPPLSAQQFKLLWTLYENQGQVIERT
                     SLVSDVWGEEQTAGVSDQALDALIRRLRDRLAVLDPSHQYINTVRGHGLR"
BASE COUNT          545 a          499 c          578 g          518 t
ORIGIN      
        1 tgccgttttt acgaacgcga aatgacagca tattccatcg cgcgtggcat tgaatacatt
       61 tatgaggaat gtccgttcgc agaaggggcg actcagatct tttataaaga gacgttgaat
      121 cagctggaaa atactcgccc gggcatcaag ctaacatttt atttacgttt tctggaagca
      181 aggcagagcg gagagttgtt cattcaacga gaagagaccc agccgcatat tcacccatgt
      241 gaaaaatgcg gtcagccgac ctctgcgccg gggctttgtt cgttctgtag aatgttggaa
      301 aaggcagacg ttgtaacaga atagaccttg ctaccgaatg acggtggcaa gtaaccccac
      361 aagaaataga ccgaaggcta cataccccgc gatctgctct gtagaaaaaa cggagaagcg
      421 cggggtttcg aatccgtagc gctctatatc gacttggatc ggaacctcga tctttccaaa
      481 caacgcatcg cggaaggtga gcacatcaac agggcggtca ttggggtgta atgccattgc
      541 ccagaagatg gctttttcca catgcgagct gatgcgtgga ttaatgtcaa tgggtggagt
      601 caggctgcgg ctatttaaga agcgtttgcg cgcctcggca ggcggttcat tcgtcaaaag
      661 atgatagaga gtcgcgccaa aggagaaaat atccgcttgt gcatcggtgt gcgtatcatc
      721 ccctccatat tgttcgagcg gcgtgtagag tgcagtgcct tgcccttgaa tgacggtgat
      781 ggtcacttcg ccaggcgcca tgattttaac caaaccaaaa tcgacgagtt taaccaagcc
      841 actaggagtc agtttgatat tgctgggctt gatatcacga tgcacgatcg gcgggtcttg
      901 atgatgtaag taattgagcg cctcagcgat ctgatttgcc caactgagca cttctcgttc
      961 tgggatgaag gtattgcttc ggcgtgcttc ctgcattagt tgacgcaggt ctttgccggg
     1021 gacgtaatcc atcacaagat agtcacgagt ttcttcggag aagaagtctg aaactttggg
     1081 gaggttgggg tggtcgaggc gtgcgagaac agaagcctcg cgaaagaatt gttcgcgggc
     1141 ttgtgcaagc acgtcggatg gaagaacctg attgtgttct acttctttga tggcacacag
     1201 acgcccttgt agacgaacat cgtctgcaag gtaaatattg cccgtgccgc cttgcccgat
     1261 ctgttcacgg acaacgtagc gttcgcgcag gatctccccg ttcaataatg attggggcaa
     1321 aacttctcct tcttggttga aagcggctgc aagagttgag tgatattttc ggcgtgtatc
     1381 acttttgcaa tcccgaaaag gggatttgca atatataatc gtgcacagaa accacttttc
     1441 tgctatatta taaacgcctt tgcaatggat agcacaatta aatctataac aaaggcgatg
     1501 aggagattat ggaacgcgaa gaggaatatc ccattcttgt tgcacaagat ggtcccttga
     1561 aggggcagcg ttggacgtta agcaagactt taatgatggg gcgtgaccca agttgtgaca
     1621 ttcaggtgca agaccgtcaa gtttctcgct ttcacgcccg cattacaccc acgcaagaag
     1681 gggtgacgct cgaagacctt ggcagcaaga acggaacgaa tcacaatgga accgagatcg
     1741 ctggtccgat catgctacag gacggcgatg cattcggaat tgcactggca caacaattca
     1801 cgtttctaac ttcagatgcc accatgccac tcgccgaaag cggaccgcgc tctgggcgct
     1861 tggtgatgga gcagaagtca cgtcaggtgt gggtcaatca acaacagttg atcccgccgc
     1921 tttctgcgca acaatttaaa ctgttgtgga ccctgtatga aaatcaaggt caggtgatcg
     1981 aacgcaccag cttggtttct gatgtttggg gtgaagagca aactgcaggc gtttcagacc
     2041 aggcgttgga tgcattgatc cgccgcttgc gtgaccggct tgccgtgctc gaccccagcc
     2101 atcaatacat caacaccgtc cgcggacatg gtctgcggtt
//
LOCUS       DAOQFT010000451         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_34821_length_2132_cov_7.400096, whole genome shotgun sequence.
ACCESSION   DAOQFT010000451 DAOQFT010000000
VERSION     DAOQFT010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34821_length_2132_cov_7.400096"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLR93_10670"
     CDS             complement(<1..670)
                     /locus_tag="PLR93_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27475.1"
                     /translation="MNTKSLAISFPLRAWLAVEVLFGVAAVLAVGLAPADTATNFAWP
                     IQPVVMAAVLGAFYITSAPLFLLPFLAKRWEMVRAMILPAALFSTVQLVATFLHWDKF
                     SVGTFPFYVWFASYLLPPPIFIAVYLWHQKQFGVESPIPAEDQIPAWLRTMLLVLGIA
                     LSLIAFIAFFFPNVIIPIFPWKLTPLTARSLSGWLVAVGTLMLSIRQGNSRVRSRLSS
                     PMLIL"
     gene            complement(679..1068)
                     /locus_tag="PLR93_10675"
     CDS             complement(679..1068)
                     /locus_tag="PLR93_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HNH27476.1"
                     /translation="MKSPTRLYTAVAGIFLLLQGVSTLTFRLVPALDAAFPQLLAVTQ
                     MVPSHSILHILTGILALIVLFRGGEKGSLWFSLGFGAFYTGLALYGFITHMPTMFHLQ
                     LFDHPFHLFLGVLGLAAGGFYFYSNRK"
     gene            complement(1065..1859)
                     /locus_tag="PLR93_10680"
     CDS             complement(1065..1859)
                     /locus_tag="PLR93_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27477.1"
                     /translation="MALVTRNMKVYFAAVGLLALWVGAWGYFVPARVDEAIPWLVPPL
                     HARFLGGMYLSGTAFMIAGIFSRRFDEVKTMVPMIAIWTGMLFIVSLFYLGEFDYSRP
                     QVWIWFGAYLAYPLIALWITWSHRTLNEPHDGTPLPAWAKNYLLVQGVLVTLLSLSLL
                     FAPDLMVSVWPWKITRLLAQIYSAPFLSYGLGSLMLSRRQTWKEARVVLIGTVVFAIF
                     VLLASIIHRELFSLSNASTWIWFGGFAIASLVLGGMTVRSLREKPE"
BASE COUNT          685 a          557 c          507 g          383 t
ORIGIN      
        1 tgagaatcaa catgggcgag gaaaggcgtg agcggacgcg gctatttcct tggcggatgg
       61 aaagcatcag cgtgcccact gccacaagcc aaccgctcag tgaacgagcc gtcagcggag
      121 ttaacttcca cgggaagatg gggatgatga cattcgggaa gaagaaagcg atgaatgcaa
      181 tcagggaaag agcgataccc agtactaaca acattgtcct caaccaggca gggatttggt
      241 cttcggcagg gatgggcgac tcaacaccaa attgcttttg atgccaaaga tatactgcga
      301 tgaaaattgg aggtggaagt agatagctgg caaaccaaac atagaacgga aaagtaccga
      361 cggaaaattt atcccaatgc aaaaaggtgg caacaagttg cacggtgctg aacaacgccg
      421 ccgggaggat catggcgcgg accatctccc aacgtttggc gagaaaaggc aacaagaata
      481 aaggagccga tgtgatatag aacgcgccta aaaccgctgc catcaccaca ggctgaatgg
      541 gccaggcaaa attcgtagcc gtatcggcag gggcaagacc tactgccaac accgccgcga
      601 ccccgaacaa cacttcaact gccaaccagg ctcgtaatgg aaatgagatt gctaaactct
      661 ttgtgttcat atgaatccct attttcgatt ggaataaaaa taaaatccgc cagccgccaa
      721 gcccagcaca cccaagaaca agtggaaggg atggtcaaaa agctgtaagt ggaacatggt
      781 cggcatgtgg gtgataaacc catacaatgc caagccggta taaaacgccc caaagccgag
      841 cgaaaaccac aggcttcctt tttcaccgcc gcggaacaag acgatcaatg ccagaattcc
      901 ggtgaggatg tgaaggatgg aatgggacgg aaccatctgc gtcacagcca gtaattgcgg
      961 aaaagccgca tccaacgcag ggacaagccg aaatgtcaac gtggatacgc cttgcaataa
     1021 cagaaaaatc cctgccacgg ctgtatataa acgagttggg cttttcattc aggtttctcc
     1081 cgcaacgaac ggacggtcat gccgcccagc acgagagatg caattgcaaa gccaccaaac
     1141 caaatccatg tagaggcgtt ggagagcgag aaaagttcac gatggatgat cgacgccaac
     1201 agcacaaaga tggcgaagac caccgttccg atcaaaacca cgcgcgcttc tttccatgtt
     1261 tggcgacgac taagcatcaa acttcccaag ccgtacgaca agaaaggtgc ggaatagatt
     1321 tgtgccaaca aacgggtgat tttccacggc cagacgctca ccatcagatc gggcgcgaat
     1381 agaagcgaca aagacaataa ggtaaccaac acaccttgca caagcagata attcttcgcc
     1441 catgccggga gtggcgtgcc atcatgcggt tcgttcaaag tgcggtgaga ccatgtaatc
     1501 cacaaagcga tcaatggata cgccaaataa gctccgaacc agatccatac ctgcgggcgg
     1561 ctgtaatcga actcgcccaa ataaaaaagc gagacgatga acaacatgcc cgtccaaatg
     1621 gcgatcatcg gcaccatggt tttcacttca tcaaaacggc gggaaaagat gccagcgatc
     1681 atgaacgccg tacccgaaag atacatgcct cccaaaaagc gcgcgtgcaa gggcggcacc
     1741 aaccacggaa tagcttcatc cacccgggct gggacgaaat atccccacgc ccccacccac
     1801 aatgccagca accccactgc ggcaaaatac actttcatgt tacgagttac caatgccata
     1861 aacacctcac taaaaagact caagaacgat gaaactgcgt atttataaac tttgcttggc
     1921 tgattataca tataaccttg gttaggttaa cagcctgtct ttggttataa agaaacccaa
     1981 tgaagaactc ccacccaact tccaagtgtg gttaaataga tttcaggagc cccacgaatg
     2041 actacagaat tcgaaacctt ccttcagaaa taccccacct acaaacacac cgaaaaaata
     2101 gatgaccttc gcaaaagcga ttattcccgc ct
//
LOCUS       DAOQFT010000452         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_34937_length_2128_cov_6.193439, whole genome shotgun sequence.
ACCESSION   DAOQFT010000452 DAOQFT010000000
VERSION     DAOQFT010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34937_length_2128_cov_6.193439"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1429
                     /locus_tag="PLR93_10685"
     CDS             47..1429
                     /locus_tag="PLR93_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27478.1"
                     /translation="MRPLEIFIPILLIFYLVWRHPRPLAIRLAPALAVLSTLLHFATE
                     GYRWQMIPLYVLTPLLALSSLSKLFGTSDWKPIASYLTVGLVALATALPILLPVPRIP
                     TPSGPFQVGTRSFEFLDESRQEIYSGKDEARRFMIQVWYPADAKASDVRAPWMENAEV
                     FAPAIAGFFKLPSFFFDHLTLTSIPAFMDADVASADEKFPVILFSHGWNGFASQNAGQ
                     ALELASRGYVVVGLQHTYGAMTTVFPDGTIAPNNPKALPEDGADPNYEEVARVLVNQW
                     AGDMAYTLDQLTAWNDTEGNPFFNKLDMERVGVYGHSTGGGAAIQFCGTDSRCDAVLG
                     LDPFMRPVSAEVLASGVSQPSFFMFSQAWADDANSPSSILFGQFIPHAADNYGVVKIE
                     GTRHYDFSDLPLFSPIAPQLGLKGPLNGKRVTEIVNSYLIDFFEMTLKNKPSELFSNV
                     SPFSEVVPIP"
     gene            1624..2010
                     /locus_tag="PLR93_10690"
     CDS             1624..2010
                     /locus_tag="PLR93_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH27479.1"
                     /translation="MNIKHIHHVAIICSDYERSKKFYTEVLGFTVKLEHYRAERQSYK
                     LDLCINDHYQVELFSFPNSAERANYPEARGLRHLAFAVEDIDAAISDLRGKGTEPEAV
                     RIDELTGKRYTFFKDPDGLPLELYEE"
BASE COUNT          529 a          466 c          530 g          603 t
ORIGIN      
        1 atataaaatt gcgtaaaatc ccagccataa atttgaaagg actcgtatgc gcccacttga
       61 aatttttatt cctattctgt taatttttta cctagtttgg cgacacccgc gcccactagc
      121 gattcgcctt gctcctgctt tggcagtgtt gtcaacttta ttgcactttg ccactgaagg
      181 ctatcgttgg cagatgatcc ctttatatgt tttgacgccg ctgcttgcct tgagtagttt
      241 gagcaaactt tttgggacaa gtgattggaa acccatcgcc tcttacttaa cggtgggctt
      301 ggttgcgctc gcgacggctc tgcctattct gttgcctgtt ccgcgcatcc ccacccctag
      361 cggaccgttc caagtaggca cacgcagctt tgaattttta gatgaatcac gccaggaaat
      421 ttattctggc aaagacgagg cacgacgatt tatgattcaa gtttggtatc cggcagatgc
      481 gaaggcaagt gatgtgcggg caccgtggat ggaaaatgct gaggtctttg cgcctgctat
      541 tgcaggtttc tttaagttgc cgtcattctt ttttgaccac cttaccttga cgagtattcc
      601 ggcttttatg gatgcggatg tcgcttcggc agatgaaaaa ttcccagtga tcttgttctc
      661 acatggttgg aatgggtttg cttctcagaa cgctgggcaa gcattggaac ttgccagccg
      721 tggttatgtg gtggtggggc tccaacatac gtatggtgcc atgacaacag tcttccccga
      781 tggaacgatt gccccgaaca acccgaaggc attgcccgaa gacggcgcag acccaaatta
      841 cgaagaggta gcgcgcgtct tggtgaacca atgggcggga gatatggcat atacgttgga
      901 ccagcttaca gcttggaatg atacggaggg aaatccgttt ttcaacaaac tggatatgga
      961 gcgagtcggt gtgtatggtc actctactgg cggcggagcg gcgatccaat tttgcggcac
     1021 agactcgcgc tgtgacgcag tgctcggctt ggatcccttc atgcgcccgg tttcagcaga
     1081 agtgcttgca agtggtgtaa gccaaccctc attctttatg ttctcacaag cctgggcaga
     1141 tgatgcgaat agccccagca gtattttgtt tggtcaattt attccacacg cggcagataa
     1201 ttatggggtt gtcaaaattg aaggaacaag gcattatgat tttagcgact tgccattgtt
     1261 ctcgccgatc gcgccacaac ttgggctgaa gggtccacta aatggaaaac gtgtgactga
     1321 gatcgtcaat tcgtatctaa tcgatttctt tgagatgacc ttgaaaaaca agccttctga
     1381 gctttttagc aatgtcagtc cattttcaga agtagttcct attccataat cgtgagaggt
     1441 agtatgaact tcaataaacg cagagtttcg ggatatttgt tgatcttcgg catgtttttt
     1501 ttggtaatcg gtattactac tgataataca attttctctt ggatatcggt tgggtttata
     1561 gtgatctcct tgtttttggg cggacgttgg atgcgccaaa gaagacgccc ataaaggttt
     1621 aagatgaata taaagcatat tcatcatgtg gctattattt gctcggacta tgaaaggtcc
     1681 aaaaagttct acacagaggt attaggcttc accgtgaaat tagaacatta ccgagcagag
     1741 cggcagtctt ataaattaga cttgtgcatt aacgaccact atcaggtcga attgttttcc
     1801 tttcctaata gtgccgagcg ggcaaactac ccagaagctc gagggttacg tcacctagca
     1861 tttgcggtag aagatattga cgctgctatc tctgacctgc gaggaaaagg aactgaaccc
     1921 gaagcagtgc ggattgatga actgaccgga aagcgctata cctttttcaa agaccccgat
     1981 ggtctaccgc tagagcttta tgaagagtga ttttttccgg ctggacggat gagccaagtc
     2041 tgtgcaccgg ggtaggtaaa gtccgccttg ggtggctatc gccaaagaac aaaagcaaaa
     2101 ggctattaac aaaatttaca actcggta
//
LOCUS       DAOQFT010000453         2126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_34992_length_2126_cov_8.542733, whole genome shotgun sequence.
ACCESSION   DAOQFT010000453 DAOQFT010000000
VERSION     DAOQFT010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34992_length_2126_cov_8.542733"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PLR93_10695"
     CDS             complement(<1..1085)
                     /locus_tag="PLR93_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNH27480.1"
                     /translation="MKILFLGTHGQFNLGDELLLETFLSQLGSEHRYAVNSYDPAFTQ
                     GMMTPKFNMEAFHTTRELPRFLKYLLTSDLLFFGGGSIIKELYASVGRNPYSTLLMIL
                     ATVTFAKKIARKKVVMSNIGVGPLMTPRGKMLARWILSQVDFLSVRDEKSHRTTLDLG
                     LSPLKVNLVPDAVFANPPEVFLPNPKPFPPSDKTRVALNLNFDIEKPEAWEPFLQSLA
                     GSLKQLHAETPLEIHALPMQSKFKANDDLSVLKEFSKRIPEIPFVLHDPQTAQEAAEI
                     ISGVDLILAERLHTLVISSILGKPFYGLVYDVKVQELVDYLGMEEYSRNINEHFCAED
                     LTKDIRSVFTDYEKISIHLTERSTNLR"
     gene            complement(1200..>2126)
                     /locus_tag="PLR93_10700"
     CDS             complement(1200..>2126)
                     /locus_tag="PLR93_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH27481.1"
                     /translation="SFRASMFDALTYAVLASVVVALWVSWFLSRRIVAPVQNLADASL
                     SLANGNYSERVQVNGSDEIAKLASRFNHMASQLEQVESMRRQLIGDVTHELRTPLTSI
                     KGYMEGLVDGVLPANAETFNQIHHEADRLSRLVDDLQELSRVEAKSYSLDLRSVSVST
                     LVQTTLKRLSPQATAKRITLRSNLPADLEPVLADEDRITQVLVNLTANAIQYTPEDGE
                     VLISAAQHGNEIHVSVKDSGIGIPQEHLANIFTRFYRVDKSRSRHAGGGSGIGLTIAK
                     HIIEAHGGRIWAESAGEGQGSTFTFSLKGVTP"
BASE COUNT          565 a          475 c          555 g          531 t
ORIGIN      
        1 atgcgcagat ttgtgctgcg ttcggttagg tggatgctga ttttctcata atcagtgaag
       61 acggaacgga tatctttggt gaggtcttct gcacagaagt gctcattgat gtttctcgaa
      121 tactcctcca tccccaagta atcaaccaat tcttgcactt ttacgtcata caccagcccg
      181 taaaaaggtt tgccaagaat agaagaaatg accagcgtgt gcaacctttc tgctaagatc
      241 aagtctacac ccgaaatgat ttctgcagct tcttgtgcgg tttgcgggtc atgcaaaaca
      301 aatgggattt cagggatgcg cttggaaaat tctttcaaga ccgagagatc gtcattagct
      361 ttgaatttac tttgcattgg caaggcatgg atttcaagcg gtgtctctgc atgcaattgt
      421 ttcagggagc cagcaaggct ttgcaggaaa ggttcccaag cctcaggctt ttcgatatca
      481 aagttgagat taagggcaac gcgagtctta tcagatggtg ggaaaggctt ggggttggga
      541 agaaagactt cgggcgggtt ggcaaagacc gcgtctggaa cgaggttaac tttcaacggg
      601 gaaagaccaa gatcgagcgt ggtgcgatgc gatttttcgt cacgcacaga gagaaaatcg
      661 acctgactga gaatccagcg agcaagcatt tttccgcgag gagtcatcaa aggaccaaca
      721 ccgatattgc tcatgaccac ttttttgcgc gcaatctttt ttgcaaaggt cacggttgcg
      781 agaatcatca acagtgtgga ataagggtta cgcccaacac tggcatatag ttctttgatg
      841 atgctgccgc cgccaaagaa aagcaaatca ctggtcagta gatatttgag aaaccgcgga
      901 agttcacgcg tggtatgaaa cgcctccatg ttgaacttgg gggtcatcat tccctgtgtg
      961 aaagcggggt cgtatgagtt gacagcatag cgatgttcgc tcccgagttg agagagaaag
     1021 gtttccaaaa gaagctcatc gccgaggttg aattgtccgt gggtgccgag gaagaggatt
     1081 ttcatagaaa agttgtgtcc gtgaattgat aataacgact tcagctatga gaaatcctga
     1141 cagaaagatt gcaaagaaaa attatttgct attttgttag gcgtaaatgt ttcagctttt
     1201 tacggtgtga ctcccttcaa actaaatgta aatgtactgc cctgcccctc acccgcagac
     1261 tctgcccaaa tgcgcccgcc gtgcgcttcg ataatatgct tggcaatcgt cagcccaatg
     1321 ccagagccac cgcctgcatg gcgggaacgg gatttatcca cacggtagaa gcgggtaaag
     1381 atatttgcaa ggtgttcctg tggaattcca atgcctgaat cttttactga aacatgaatt
     1441 tcatttccat gttgggcggc tgagatcaac acctcaccat cttcgggcgt gtattgaatc
     1501 gcattcgctg tcaggttgac caatacctgc gtgatgcggt cttcgtcagc gaggacaggc
     1561 tcaaggtcag cgggcaggtt cgagcggagg gtgatgcgtt ttgccgtcgc ctgaggagaa
     1621 agtcgtttta gggtggtctg caccagagta gaaacgctca cagagcgaag atccaaagaa
     1681 tatgacttgg cttcgacacg gctcaattct tgcaagtcgt cgacgagacg cgagaggcgg
     1741 tccgcttcgt ggtggatttg gttgaaggtc tcggcattgg caggcaagac cccatccacc
     1801 aaaccttcca tatatccttt aatagaagtg agcggggtgc gcaattcgtg agtcacatcg
     1861 ccaataagtt gacggcgcat ggactccact tgttcgagct gtgatgccat gtgattgaaa
     1921 cgtgacgcga gctttgcaat ttcgtctgag ccatttactt gcacacgttc agagtaattt
     1981 ccattagcaa ggctcaagga tgcatcagca aggttttgca ccggggcaac aatacgacga
     2041 cttaaaaacc aactgaccca caatgccaca acaacagaag caagcacggc ataggtcagc
     2101 gcatcgaaca tgctggcacg aaaact
//
LOCUS       DAOQFT010000454         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35061_length_2124_cov_4.879169, whole genome shotgun sequence.
ACCESSION   DAOQFT010000454 DAOQFT010000000
VERSION     DAOQFT010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35061_length_2124_cov_4.879169"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /gene="ffh"
                     /locus_tag="PLR93_10705"
     CDS             complement(<1..1189)
                     /gene="ffh"
                     /locus_tag="PLR93_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNH27482.1"
                     /translation="MFETLTGRLNQIFDNLRKRGKLSEADVDAAMKEVRLALLEADVH
                     FSVVKTFIAKVRERAVGAEVSKALNPGQQVIKIVNEELVATLGEPAPLNLTGPKPRVV
                     MMVGLQGAGKTTVTGKLARLLKSKGERILMVAGDPYRPAAVKQLQQLGERVGVDVEAD
                     PSIKPQELAKRALDKAEKGGYSLMLVDTAGRSQMDAQLMDELKAIHANVKPVDTLLVI
                     DSMIGQEALNIAQGFKETIDLTGLILTKMDGDSRGGAAISIRSVTGVPIKFIGTGEKL
                     DALETYDPSRLSSRILGMGDLIGLIEKAEAAYDAETAEKQAKKLMKGDFSLEDWLEQM
                     NQMKKMGPLSQIMEMLPGQMGQAARGIDPSLVEKQFKQSEAIINSMTLKERRDPDILN
                     ASRR"
     gene            1440..>2124
                     /locus_tag="PLR93_10710"
     CDS             1440..>2124
                     /locus_tag="PLR93_10710"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27483.1"
                     /translation="MATEAIVNDRKEIFGWAMYDWANSAFSTTVGTVFLGPYVASLAR
                     AAAEAAGTSTVSFLGIPVAPDSFLPYCISFSVGMQVLFLPILGAIADYSHLRKTMMRI
                     FATIGAIATILMFMLTGGLWWLGGVLFILANLTFGAAIVFYNAYLPDIASEEERDRVS
                     SYGWAMGYLGGGILLALNLAFYIFSDKIGVPGDLAVRINLASAGIWWLGFSFFTWNRL
                     RSRHASNSLP"
BASE COUNT          465 a          520 c          548 g          591 t
ORIGIN      
        1 ggcggcggga ggcattcaaa atatcgggat cgcgacgttc tttcagggtc atcgaattga
       61 tgatggcttc tgattgtttg aactgttttt caaccagcga agggtctatg ccgcgggcgg
      121 cttgccccat ttgcccgggc agcatttcca taatctgcga aagcggtccc atctttttca
      181 tctggttcat ttgttcaagc caatcttcaa gcgagaaatc gcctttcatc aatttcttgg
      241 cttgcttttc tgcggtctcg gcatcgtagg cggcttcggc tttttcgatc aagccgatga
      301 ggtcacccat gcccaaaata cgtgaagaca aacgtgatgg gtcataggtt tccaatgcat
      361 cgagcttttc acctgtacca ataaatttga tcggcacgcc ggtcaccgaa cgaatagaga
      421 tggcagcacc accacgtgag tcgccgtcca ttttggtgag gataagaccc gtcaggtcga
      481 tggtttcttt gaaaccttga gcaatgttca aagcttcctg accaatcatg gaatcgatga
      541 caagcagagt atccaccggc ttgacattgg catggatggc tttcaattcg tccatcaact
      601 gcgcatccat ctgggaccga ccggctgtat cgaccaacat cagactgtaa ccgccctttt
      661 cagctttatc gagtgcgcgc ttcgcgagtt cctgcggctt gatggatggg tctgcttcca
      721 catccacgcc cacacgttcg ccaagttgct gcaactgttt gaccgccgct gggcgatatg
      781 ggtcgccagc taccatcaaa atacgctcac cttttgattt gagtaaacga gccagtttgc
      841 ctgtaactgt cgttttacca gcaccttgca aaccgaccat catcaccaca cgtggtttgg
      901 gtcccgtgag gtttagcgga gcgggttcac ccagggtggc gacaagttcc tcgttgacga
      961 tcttgatgac ttgctgaccc gggttcaacg ccttcgaaac ttctgcaccc actgcacgtt
     1021 cgcggacttt ggcaatgaaa gttttgacca cgctaaaatg cacatccgct tcgagcaagg
     1081 cgaggcgcac ttctttcatg gcggcatcga catcggcttc ggagagcttt ccgcgtttgc
     1141 gcaaattgtc gaagatttgg ttgagacgtc cggtaagagt ttcaaacata ggggtataaa
     1201 tgactgccat taagacaaaa tggcaagatt ccttttcagt gtaagaagag gcattttagc
     1261 gcggagcagg ttgagggtca agcaggaggc gcggttctta atccataaaa attggcaaaa
     1321 ccattcacag agacgagggt ttttattctc acaatgttaa ccgaacatct gtgggttgtc
     1381 cgtatataat acgggcagac gtgaatccat aacatcaatc cagttcgagg agaagaggca
     1441 tggcaacgga agcgatcgtc aatgaccgga aagagatttt tgggtgggca atgtacgact
     1501 gggcaaactc ggcttttagc acgactgttg gaaccgtatt tttaggaccg tatgtggcaa
     1561 gtttggcgcg cgctgcagca gaagcggctg gaaccagcac cgtttcgttc ttaggaattc
     1621 ctgttgcacc agattctttt ctaccgtact gtatttcatt ttctgtaggc atgcaggttt
     1681 tgttcctgcc gatccttggt gcaattgcag attactcgca cttgcgcaag accatgatgc
     1741 gcatctttgc gaccatcggc gcgattgcca ctattttgat gttcatgctc acaggcgggc
     1801 tttggtggtt aggcggtgtg ctctttattc ttgccaacct gacctttggt gctgctattg
     1861 ttttctataa cgcctattta cctgatattg ccagcgaaga agaacgagac cgtgtctctt
     1921 cgtatggttg ggcgatgggc tatttgggtg gcggcatttt gctggcgctt aacctggctt
     1981 tttatatttt cagtgacaag atcggtgtgc ctggcgattt ggcagtgcgc atcaaccttg
     2041 catcggcggg tatttggtgg cttggcttct cgttctttac ttggaatcgc ttgcgctcac
     2101 gccatgcgtc gaactcctta cctg
//
LOCUS       DAOQFT010000455         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35080_length_2123_cov_6.266441, whole genome shotgun sequence.
ACCESSION   DAOQFT010000455 DAOQFT010000000
VERSION     DAOQFT010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35080_length_2123_cov_6.266441"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLR93_10715"
     CDS             complement(<1..520)
                     /locus_tag="PLR93_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH27484.1"
                     /translation="MAKNIEDYYAPWVKGIPMYISEHIELAWRKPELHRMMSNENPLP
                     PSEKVLEAMFKYAKMTNRYPDQGLVVRQKIAEINGVAGPQNVMIGNGSSEVYDNIFRM
                     FLEPGDEVIQHTPCFGIYGLRGTLLGAKMVSVPMIYKDHKLIFDHEGLMNAVTDKTKI
                     IVIPNPNNPSGNF"
     gene            complement(602..1477)
                     /locus_tag="PLR93_10720"
     CDS             complement(602..1477)
                     /locus_tag="PLR93_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HNH27485.1"
                     /translation="MSIVNAKEIMIPAAKEGWAVGAFNVTDLLQFEAVIDAAIEKKAP
                     VIVQTSVKPSQFLGTNMMVAIYRTLAESAPVPVCLHLDHSTDINYCKKCADAGYTNIM
                     IDASKQSYEENIRQTKEVVDYAHSVGNISVEGELGTVGGVEDQIKVAEDEAQLANPEQ
                     SIEFVERTGVDIFAPAIGTAHGVYKTKNPKIDFERMATIHKMLNSNGIKTPLVVHGGT
                     GLPDDYVAKLLAAGGAKFNVSTELKHTLINAKWEYLSAHRDEYDPGKLDVFVRDATRK
                     AVMHWMDKLGCNGKA"
     gene            complement(1592..>2123)
                     /locus_tag="PLR93_10725"
     CDS             complement(1592..>2123)
                     /locus_tag="PLR93_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNH27486.1"
                     /translation="RRVEMLTKTAKAKGAHFPEDWAFIQKKMREAEKALLKDPYKPTP
                     CHNDLLNLNWLDEEVAGDIGEIRLLDWEYAGMGDIFFDLGNFSHHHRLNEDQIRIFLT
                     AYFGKFTPKHYARLRIMWAMSELHEAMWGTTQSVISTLEEDFQGYANLWFGRYRMHVT
                     DYRWEQWLKDASKKGK"
BASE COUNT          466 a          532 c          541 g          584 t
ORIGIN      
        1 tgaagttgcc gctggggttg ttggggttcg ggatgacgat gatcttggtc ttatcagtga
       61 ctgcgttcat cagaccttca tggtcgaaga tgagcttgtg atctttatag atcatcggca
      121 cagagaccat tttggcgccg agcaaggttc cgcgcagacc gtaaatgccg aagcagggtg
      181 tgtgctgaat gacttcatcg cccggttcga ggaacatgcg gaagatgttg tcatacactt
      241 cgctggagcc gttgccgatc atcacgttct gcggaccagc cacgccgttg atctcagcaa
      301 ttttctgacg caccactaaa ccctgatcgg gatagcgatt ggtcatcttg gcgtacttga
      361 acatcgcctc tagcaccttt tcagaagggg gcagcggatt ttcattggac atcatgcgat
      421 gcagttcggg tttgcgccaa gccaactcaa tgtgctccga aatgtacatc ggaatgccct
      481 tcacccatgg ggcgtaataa tcttcgatat ttttagccat gttacttgtt ctcctaaatt
      541 atttttacga attatcacga tggacgatct tatctgccgg acatcgttga ttgtcttatg
      601 attaagcctt gccgttacag ccgagcttgt ccatccagtg cattacggct ttgcgggtcg
      661 catcgcggac gaagacatcg agcttgccgg ggtcgtattc atcgcggtgg gcgctgagat
      721 attcccattt ggcgttgatg agggtgtgct tcaactcggt ggagacgttg aacttcgcac
      781 cgccagccgc caacaacttg gcaacataat catcgggcaa gccggttccg ccgtggacga
      841 cgagcggggt cttgatgcca ttgctgttca gcatcttgtg aatggtcgcc atgcgttcga
      901 aatcgatctt gggattcttg gtcttgtaca cgccgtgcgc ggtgccgatg gcgggagcga
      961 aaatgtccac acccgtgcgt tcgacgaatt caatggattg ttcggggttg gcgagttgtg
     1021 cttcatcttc cgcgaccttg atctggtctt ccacaccacc aacggttccg agttcgcctt
     1081 ccacagaaat gttgccaacg ctgtgagcat aatccaccac ttctttggtc tggcggatgt
     1141 tctcttcgta ggactgtttg gaagcgtcga tcatgatgtt ggtataccca gcatccgcgc
     1201 acttcttgca gtagttgata tcggtgctgt gatcgaggtg caaacacacc ggcacaggcg
     1261 cagattcagc caatgtgcgg tagatcgcta ccatcatatt agtgccgagg aactgtgaag
     1321 gcttcacaga agtctgcaca atgacagggg ctttcttttc aattgccgcg tcgatcaccg
     1381 cttcaaattg cagcaagtcg gtgacgttga acgcgcccac agcccaacct tcttttgccg
     1441 cagggatcat aatctctttt gcatttacga tagacatttg gtactccttg gttttttctt
     1501 ttcaggtttc gagtttgtat cgaaagagga aaaatgaaaa taaactatct tctttcctct
     1561 ttcgccaatt accaactact tatgaactaa cttacttgcc cttcttcgac gcatctttca
     1621 accactgttc ccagcgatag tcagtgacat gcatgcgata gcgcccgaac cacaaattgg
     1681 catagccttg gaaatcttct tccaacgtgg agatgaccga ttgggttgtg ccccacatgg
     1741 cttcgtgcaa ctcagacatc gcccacataa ttctcagccg cgcataatgc ttgggggtga
     1801 acttcccaaa ataagcggta aggaagatgc ggatttgatc ctcgttcaaa cgatggtggt
     1861 gcgagaaatt cccgaggtca aagaaaatat cgcccatgcc tgcatattcc caatcgagca
     1921 agcggatctc gccaatatcg cccgcaactt cttcatccaa ccagttgagg ttgagcaaat
     1981 cgttgtggca gggggtgggt ttgtagggat ctttcaacaa tgctttttct gcttcgcgca
     2041 ttttcttttg aatgaaagcc caatcttcag ggaagtgcgc gcccttggct ttggcggttt
     2101 tggtgagcat ttccacgcgg cgg
//
LOCUS       DAOQFT010000456         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35428_length_2111_cov_3.898833, whole genome shotgun sequence.
ACCESSION   DAOQFT010000456 DAOQFT010000000
VERSION     DAOQFT010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35428_length_2111_cov_3.898833"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLR93_10730"
     CDS             <1..614
                     /locus_tag="PLR93_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH27487.1"
                     /translation="DNFNLQTSHVIPALIRKAVEASERAPYGLAKKMMLVQSQTYRQQ
                     YGFNAIYLLPVNLYGPRDNFNLQTSHVIPALIRKAVEACERGDKELPAWGDGSPTREF
                     LYVEDAADGLVTAAEKYNGDLPVNLGSGYEISIKDLTELVAKLTGFTGKIVWQTDKPN
                     GQPRRGLDVTRAKELFGWSAQVSFEEGMKRTIEWFKANRDKIK"
     gene            617..1462
                     /locus_tag="PLR93_10735"
     CDS             617..1462
                     /locus_tag="PLR93_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27488.1"
                     /translation="MTEIVKHEPHTVYIKPSKGLAALNLRDLWVYRELIYFMVWRDIK
                     VKYKQTLLGMAWAVVQPVMTMLVFTFLFDRVAKLPTEGVPYPVFSFTALLPWGLFVTA
                     LNQGSRALVAHQNMVTKIYFPRLILPTASVFAGLVDFAIAFVILVILMFYYQVTPAWH
                     LLWTLPLFLLLAIVTALGIALWLSAINVQYRDVNQALPFLTQFWLFATPVAYSASVIS
                     EKWQVVYSLNPMAGVVNGFRWALLGVGNGPDISLWVSTGISILIFITGLFYFKSMEKT
                     FADTI"
     gene            1524..1886
                     /locus_tag="PLR93_10740"
     CDS             1524..1886
                     /locus_tag="PLR93_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNH27489.1"
                     /translation="MAMIQKFEDIQAWQEARTLVKMVYQLTNKEKFSKDFGMRDQIRR
                     AAVSVMNNIAEGFDCESKAEFARFLGIARRSAVEVQSLFYAALDVEYIDQNEFDAHYE
                     QARKAKALIGGFKRSLAK"
     gene            1921..>2111
                     /locus_tag="PLR93_10745"
     CDS             1921..>2111
                     /locus_tag="PLR93_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27490.1"
                     /translation="MTTAISVKNIGKQYKIGVAETKFRYNMLRDVIVDTVAAPIRLAK
                     AMIGKSDRRMNQNYVWALK"
BASE COUNT          517 a          514 c          518 g          562 t
ORIGIN      
        1 gtgataactt taatttgcaa acttcacatg tgattccagc gctcatccgc aaagccgtgg
       61 aagcaagtga gcgggctccc tacggacttg ccaagaagat gatgcttgtt cagtctcaga
      121 cctatcgcca gcaatatggt ttcaatgcca tttatctgct gcctgtgaat ctttacggac
      181 cgcgtgataa ctttaatttg caaacttcac atgtgattcc agcgctcatc cgcaaagctg
      241 tggaagcatg cgagcgtggt gacaaggaat tgcccgcctg gggtgacggt tctcctaccc
      301 gcgaattcct ttacgtagaa gacgctgctg atggtcttgt cactgctgct gaaaaatata
      361 acggtgacct gcccgtcaac ctcggctcgg gttatgaaat ctccatcaaa gacctcaccg
      421 aactggttgc caagttgact ggtttcactg gcaagatcgt ctggcagaca gacaagccca
      481 atggtcaacc ccgccgcgga ctggacgtga cccgtgccaa agaactcttc ggctggagcg
      541 cgcaagtctc attcgaagaa ggtatgaagc gcaccattga gtggttcaaa gccaatcgag
      601 ataagatcaa gtaattatga ccgaaatcgt aaagcacgaa ccccatactg tttatatcaa
      661 gccctctaaa ggacttgctg cgttaaatct cagagacctt tgggtatacc gtgaattgat
      721 ctacttcatg gtttggcgtg atatcaaagt gaaatacaag caaacattgc ttggcatggc
      781 ttgggcggtt gtccagcctg tgatgaccat gctggtcttc actttccttt tcgaccgggt
      841 agcaaaactt cccactgaag gcgtgcctta ccccgtcttt tcgtttactg cgcttttgcc
      901 atggggcttg ttcgtgacgg cgcttaatca gggcagccgt gctttggtgg cacatcaaaa
      961 catggtaacg aagatctact tcccccgcct cattcttccc acagcttccg tctttgccgg
     1021 gcttgtagat tttgccattg cctttgtaat ccttgtgatc ttgatgttct attatcaagt
     1081 cacgcccgcc tggcatttgc tttggacact tccactcttc ttgctgcttg ccattgtgac
     1141 agcgcttgga attgctctct ggctgtctgc gattaacgtc cagtatcgtg acgtgaacca
     1201 agccctcccg tttctgactc agttttggct gtttgctaca cccgtagcct attctgcctc
     1261 tgtgatctct gagaagtggc aggtggtgta ctcgctcaac ccgatggcag gtgtggtcaa
     1321 tggctttcgc tgggcattgc tcggcgtcgg caatgggccc gatatctccc tttgggtctc
     1381 aaccggaatt tcaattttga tctttatcac aggtctcttc tacttcaaga gcatggaaaa
     1441 gacctttgcg gacaccatat aaagtgtcag gtgcgaggtg tcaggtatca ggcggaaata
     1501 ccgtctgagt atcggagaaa atcatggcga tgatccagaa gtttgaagat atacaagcct
     1561 ggcaggaagc acggacactg gtaaaaatgg tttatcaact gacgaataag gaaaaattct
     1621 cgaaagattt tggaatgaga gatcagatca ggcgtgcagc ggtttcagta atgaacaata
     1681 ttgccgaagg tttcgattgt gagtccaagg ctgagtttgc ccgttttctt ggcattgcca
     1741 gacgttccgc tgtagaagtc caatccctgt tttatgcagc attggatgtg gaatacattg
     1801 accagaatga gtttgatgcg cattacgagc aagcacgtaa agctaaagcc cttattggcg
     1861 gattcaaacg ctcgctggcg aaataacctg acacctgata cttgacccct gacacctact
     1921 atgacaactg caatcagcgt aaaaaatatc ggtaagcaat acaagatcgg cgtagcggaa
     1981 accaaattcc gctataacat gctgcgcgat gtgatcgtgg acacggttgc cgcgcccatc
     2041 cgtttggcga aggcaatgat cggaaaatct gaccggcgca tgaaccagaa ttatgtttgg
     2101 gcgttgaagg a
//
LOCUS       DAOQFT010000457         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35448_length_2110_cov_6.309489, whole genome shotgun sequence.
ACCESSION   DAOQFT010000457 DAOQFT010000000
VERSION     DAOQFT010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35448_length_2110_cov_6.309489"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLR93_10750"
     CDS             <1..1048
                     /locus_tag="PLR93_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH27491.1"
                     /translation="GDFYSSGHYLELEENKKVKFRWYSSIDPAPTEVTVSLTEKDGGV
                     NIRMDHEVPSDEAWKKMAEGFRQNWQDSLRNLKSVLETGVDLRIAERPMLGIVPGDFS
                     EEQAKALGVPVLQGMRLDATVEGMGAKRAGIERDDVLVSMAGKPITNDFTSLQLAIAG
                     KKGGDVIEVEFYRGAEKKKVSMELTKRPMADVPFNSVELAKKARALMEPALAEIEKCF
                     EGYSDEQAMKRPEPNEWSALEVVAHLIQGERFNVIYLASLVDGYEAVSDGFGSNISES
                     MTAMVKANPSIKLMMEALRRSVDEILLFVEMLPADFVANKGSYYRFGSGLLQPEFHLN
                     AHTDQLRKALEAAK"
     gene            1177..1662
                     /locus_tag="PLR93_10755"
     CDS             1177..1662
                     /locus_tag="PLR93_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27492.1"
                     /translation="MKQNIKKLSVLAIILMLTLSACKANVSRNNDGSLSVETSVTQAQ
                     LQEIISSSIADPLIKSLTVTLQQGYVSVIGERERLNDASKTDSMSFRLDLGVSNGELT
                     ASISDAQIDGVSIEQNRVDHWNQTIANRIANIGSRNENAQLQSVSITPEQVLMTWTVV
                     R"
     gene            complement(1703..>2110)
                     /locus_tag="PLR93_10760"
     CDS             complement(1703..>2110)
                     /locus_tag="PLR93_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27493.1"
                     /translation="MVMLVGYFLWPWMSRKYSGRVVLLATTLGLAFYPALTAATVKVE
                     WIIVYAGIAGLFQAGLDLVFFDELMKTVPPEYSATFVSFSQSLQYFSAVIAPTVGTWF
                     ADSIGLGGALWVSAGLRLLGFVLFLIPDRRKVT"
BASE COUNT          631 a          480 c          520 g          479 t
ORIGIN      
        1 tggcgatttc tattccagtg ggcattatct tgaattggaa gagaacaaga aagtgaaatt
       61 ccgctggtat tcgagcattg accccgcccc taccgaagtg acagtcagct tgaccgagaa
      121 agatggagga gtgaacatcc gtatggatca tgaagtgccg tcggatgaag cttggaagaa
      181 aatggcagag ggctttcgcc aaaattggca ggatagcctg cgaaatttaa aatccgtgct
      241 cgaaacaggt gttgacttac gcattgcaga acgtcccatg cttggcattg tgcctgggga
      301 ttttagtgaa gaacaggcaa aagcgcttgg ggttcccgtc cttcaaggaa tgcggttaga
      361 tgcgacagta gaaggtatgg gcgccaaacg agccgggatt gagcgggatg atgtcctcgt
      421 cagcatggca ggcaaaccca tcaccaatga cttcaccagc ttgcaactcg ccattgccgg
      481 gaaaaaaggc ggggatgtaa ttgaagttga gttttatcgt ggcgcagaaa agaaaaaggt
      541 ttccatggaa ttgaccaaac gtcccatggc agatgtgccg ttcaactcag ttgagttggc
      601 aaagaaagcc cgtgcgctga tggaacccgc cctggcagag atcgagaaat gttttgaagg
      661 gtattcagat gaacaagcca tgaaacgccc cgagccaaac gaatggagtg cgctggaagt
      721 ggtggcgcac ctcattcaag gtgaacggtt caatgtcatt tatcttgcca gcttggtcga
      781 tgggtatgaa gccgtgtctg atgggtttgg gagcaatatc agtgaatcga tgacagcaat
      841 ggtgaaagcc aacccgtcca tcaaactgat gatggaggct ttacgccgca gtgtggatga
      901 gattctgctc ttcgtagaaa tgctcccagc agattttgtg gcgaacaaag gcagttacta
      961 ccgctttggc agtgggttgt tgcaaccaga atttcatttg aatgcccata ctgaccaact
     1021 tcggaaagca ttggaagccg cgaaataagt ttttactgtt tataaaataa aaagccgagt
     1081 ctctcaaaga ctcggctttt tttacagaga atttacaaac taatattgaa cagaaaacaa
     1141 aggagtggga tactacaaaa cattggagaa taaaacatga aacaaaatat caaaaaatta
     1201 tctgtccttg ccataatctt gatgcttaca ttgagcgctt gcaaagccaa tgtgtctcgt
     1261 aacaatgatg gctctttatc tgttgaaact tctgtaacac aagcccagtt gcaggagatt
     1321 atttcttctt ccattgcaga cccgcttatc aagagtctga ccgtgacgct acaacaggga
     1381 tatgtttctg tgattggcga acgcgaacgt ttgaacgacg ccagcaagac cgactcaatg
     1441 agtttcaggc tcgacctcgg cgtgagcaat ggcgaattaa ccgcctccat ttcagatgca
     1501 caaattgacg gagtgtccat tgagcaaaac cgcgttgatc attggaacca gaccattgcc
     1561 aaccgcatcg caaacattgg cagccgaaat gaaaatgccc aattacagtc tgtatcgatc
     1621 actcccgaac aagtcttaat gacttggact gttgtccgat aataaaaaga agtccatgag
     1681 atgaactcat ggacttcttt ttttaagtca ctttgcgccg atcaggtatt agaaagagca
     1741 caaaccctag caatcgcaga ccagcactca cccacagcgc accacccaac ccgatagaat
     1801 ccgcgaacca ggtaccgacc gtcggagcga tgacagcgga gaaatattga agggactgag
     1861 aaaaagatac aaaggtggcg ctatattccg gcgggacggt tttcatcaac tcatcgaaga
     1921 agaccaaatc caaaccggct tgaaagagcc cggcaatgcc cgcatataca atgatccact
     1981 ccactttaac tgtggcagcg gtcaaagcgg ggtaaaacgc cagccctaat gtggtagcaa
     2041 gcaataccac ccgtccgcta tatttgcgag acatccacgg ccagagaaag taacctacca
     2101 gcatcaccat
//
LOCUS       DAOQFT010000458         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35517_length_2108_cov_4.925475, whole genome shotgun sequence.
ACCESSION   DAOQFT010000458 DAOQFT010000000
VERSION     DAOQFT010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35517_length_2108_cov_4.925475"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..816
                     /locus_tag="PLR93_10765"
     CDS             217..816
                     /locus_tag="PLR93_10765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH27494.1"
                     /translation="MPLRYLALGDSYTIGESVEEAERFPNQLASLLANEGVTVDVKII
                     ARTGWTTDELWRGIQAEVVEPPYDMVSLLIGVNNQYRGRGLEEYREGFIFLLNKAIEY
                     AGGDVKRVVVFSIPDWGVTPFAYGRDTTQIANEIDAFNKVNLEESTKAGAHYVDVTPS
                     SRLALNDKTLIASDGLHPSGKMYAEWAQSALPIALNILK"
     gene            848..1252
                     /gene="mce"
                     /locus_tag="PLR93_10770"
     CDS             848..1252
                     /gene="mce"
                     /locus_tag="PLR93_10770"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNH27495.1"
                     /translation="MPQIKAINHVAVVVEDMEKSLAFWRDALGIKLHELRDVPAEKSQ
                     VAFLPLAGAEVELVMPTTDDSGIAKYLAKRGQGMHHLCLEVDDIEGMMAQLKAKNIRL
                     INEEPRTAADGKKYAFIHPESTGGVLVELYQI"
     gene            1253..>2108
                     /gene="tilS"
                     /locus_tag="PLR93_10775"
     CDS             1253..>2108
                     /gene="tilS"
                     /locus_tag="PLR93_10775"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNH27496.1"
                     /translation="MSSNVETIMVEECGLVKDRPVIVGVSGGPDSLCLMETLRRAGYH
                     LIIGYFDHKLRPESALDARMVEKAAVRLGLLCILGGMDVRSYAEEKKLSIEEAARTLR
                     YRFLFELARKYKAQAVAVGHTADDQVETVLMHFLRGSGISGLKGMSYRSVIKSFDPDI
                     PVVRPLLDMWREETVLFCAVNGLRPSYDSSNESLNFQRNRIRHLLIPSLETYNPKFRE
                     TVLRMSHSLKGDYAFVMETLEHAWGNVVVSANDEIITFDVLRLSSMPIGLQRNLIRHA
                     MQTLLPGVD"
BASE COUNT          534 a          484 c          525 g          565 t
ORIGIN      
        1 tctcctcttg tgtgtgcaag ggcgtgaccg ggcagtcatg cccttgccta tttaagacga
       61 tagacaatgg accacggaca aaaatttatc atcgtcaatg gaccatcgtc catcgtcctt
      121 tttgatatac ttgccttcgt gaagaaattc gcgatcttgt tttgtctact tcttattagt
      181 tgctcgacca aatcaacccc tacaccggag actgtcatgc ccctgcgata cttggcttta
      241 ggtgactctt ataccatcgg tgaaagtgtg gaagaagcag agcgcttccc aaatcaactt
      301 gcatcattgc ttgcaaatga aggtgtgacg gttgatgtga aaatcatcgc tcgcaccggc
      361 tggacgacag atgagctttg gcgcggcatt caagccgaag tggtggagcc gccctatgat
      421 atggtctctt tactgattgg cgtgaataat cagtatcgtg gcagagggct tgaagaatat
      481 cgcgaagggt tcatattttt actaaataaa gccatcgaat atgccggcgg agacgtaaaa
      541 cgagtcgttg tcttctccat tccagattgg ggtgtcactc cgttcgcata tggcagagac
      601 accacccaga tcgcaaatga aatcgacgct tttaataaag ttaacctcga agagtccacc
      661 aaagcaggtg ctcattatgt agatgtaacc cctagttccc gcttagcgct caacgataaa
      721 accctgattg catccgatgg attgcatccc tctggcaaaa tgtatgctga gtgggcacaa
      781 tctgcgttac cgattgcgtt gaatatcttg aagtagaatc ttttcataaa tcaaaaacgg
      841 agaatcaatg cctcagatca aagctatcaa tcatgttgcc gttgttgtcg aggatatgga
      901 aaagtcgctt gcgttttggc gcgatgccct gggaattaaa ctacatgaat taagagacgt
      961 gcccgccgag aaatctcaag tggcgtttct gcctttggct ggcgctgaag tggaattggt
     1021 gatgcccacc accgacgact ctggcattgc caagtatctt gccaaacgtg ggcaagggat
     1081 gcatcattta tgccttgaag tggatgatat tgagggcatg atggcgcaat tgaaagcgaa
     1141 aaacattcgg ctcatcaatg aagaaccgcg caccgcagca gacgggaaga agtatgcttt
     1201 catccaccct gagtcaacgg gcggcgtttt agtggagttg tatcaaatct aaatgtcgtc
     1261 aaacgttgaa acaatcatgg tagaggaatg tggtctggta aaagaccgtc ctgtcatcgt
     1321 cggtgtttcc ggcggaccag acagtctctg cctaatggaa acgcttcgcc gcgcagggta
     1381 ccatctcatc atcgggtact ttgaccacaa actccgccct gagtctgcac tagatgcgcg
     1441 catggtagaa aaagctgccg tgcggttggg cttgctatgt attctggggg gcatggatgt
     1501 gcgttcctat gcagaagaaa agaaactctc tattgaagaa gccgcgcgga cgttaagata
     1561 tcgcttcctc tttgaacttg ctagaaaata taaggcacaa gccgttgctg ttgggcacac
     1621 agcagatgac caagtagaaa cggtccttat gcattttttg cgtggaagtg gtatttcggg
     1681 cttgaagggt atgtcgtacc ggtcggttat caaatccttt gatccagata ttccagtggt
     1741 tcgtccgtta ttggatatgt ggcgcgaaga gacggttctg ttctgtgcgg ttaatggctt
     1801 acgcccaagt tacgactcga gcaatgagtc tttgaacttt caacgcaacc gcattcggca
     1861 tttgctcatt ccctcacttg aaacatacaa tccaaaattt cgcgagacgg ttttgcgcat
     1921 gtcacattcc ctgaagggag actatgcctt tgttatggaa acattggaac atgcttgggg
     1981 caatgtcgtt gtttctgcga atgatgagat tatcaccttc gatgttttgc gcctatcttc
     2041 catgccgatc ggtctccaac gtaacctgat tcgccatgcc atgcagactc ttctcccagg
     2101 cgtggaca
//
LOCUS       DAOQFT010000459         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35541_length_2107_cov_7.690546, whole genome shotgun sequence.
ACCESSION   DAOQFT010000459 DAOQFT010000000
VERSION     DAOQFT010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35541_length_2107_cov_7.690546"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            560..1831
                     /locus_tag="PLR93_10780"
     CDS             560..1831
                     /locus_tag="PLR93_10780"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNH27497.1"
                     /translation="MSPKSKPSVKKVVLAYSGGLDTSVIVPWLKENYGCEVVCFCADV
                     GQGDEDLAGLKDKAIKSGASEFVIHDMKEEFVSEYLFPMLKAGAVYEHKYLLGTSVAR
                     PLIAKHLVEVAHETGADAIAHGATGKGNDQVRFELTVMALDPRLKIIAPWREWEIRSR
                     EDALNFAAKHNVPVTATIKSIYSRDSNLWHLSHEGGLLEDPWNEPEEIMYQMSTSPEN
                     APEQGEYVEIEFESGNPIAVNGEKMSPAKIVSTLNAIGGNHGIGRVDLVENRLVGMKS
                     HGVYETPGGTILLYAHRELESLILDRETLHYKQTIAVKYAEITYNGLWFTPLREALDA
                     FVNSTQGPVTGTVRLKLYKGNIISAGRKSKFSLYREDFATFGQEDVYDQSHAEGFIRL
                     YGLSMKVRAMNGLPVSGLDMPKPDYSKFKRD"
     gene            1841..>2107
                     /locus_tag="PLR93_10785"
     CDS             1841..>2107
                     /locus_tag="PLR93_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNH27498.1"
                     /translation="MTLWGGRFSQKLDDLAWALNASLPVDQRMALQDVEGSIAWAVAI
                     HNAGILTDEEHASISTGLGTVRKEFASGEFIFQPSDEDIHTAVER"
BASE COUNT          545 a          495 c          540 g          527 t
ORIGIN      
        1 agcagcaatg gggtaaataa aaacgccccg cgttttccgc gaggcgttgt gtgttctgaa
       61 atccgttctg acggttattt ctgaccaagc acaaatgcct cgcggtgtgt accggaggta
      121 gtggtcatgg tcatacccaa gccgtgcttg gtgttcgtca gaatgttgaa cataattggg
      181 cggtattcta tgtgagtgat cgagatgtgt caagggatga atttattcaa ttacccgaat
      241 gggtacttct tgaaaattga tctctcaaaa ccattgactc attttgagtc ggtagtagaa
      301 ttgcgcgaaa tgtttagcct gtttgtttta cctcgtacac gtcgtaccaa gcctacttcc
      361 cagagaaggt tgggagcttt tcgtttggac gacacgaaca cgagcgtgaa ctaacacacg
      421 cacagtcgac caaaacagcc catccttctc ggatgggctg tttttatttt ccggaatccg
      481 gaagttgttt ttccgtggtt ttagaaacaa aataattctc aaagtagaac tttgggagtc
      541 caaaaatcaa ggagtaaaaa tgagtccaaa gtcaaaacct agtgtgaaga aagttgtctt
      601 agcctactcg ggagggttgg atacatctgt gatcgtgccg tggttgaagg aaaactatgg
      661 ctgcgaagtg gtttgtttct gtgccgatgt ggggcagggt gacgaagatt tggcggggtt
      721 gaaagacaaa gccattaaga gtggcgcgag tgaatttgtt attcatgata tgaaagaaga
      781 atttgttagt gagtatcttt tcccgatgct caaagcgggg gcggtctatg aacataagta
      841 tttattgggc acatcggttg cccgtcctct tattgcaaaa caccttgtag aagtggcgca
      901 cgaaaccggc gcggacgcga ttgcacatgg cgcaactggc aaaggcaatg accaggtacg
      961 ttttgaactg accgtgatgg cacttgaccc gcgccttaag attatcgctc cgtggcgcga
     1021 atgggaaatt cgttctcgcg aagatgcact caattttgca gcaaagcaca acgtacctgt
     1081 gaccgccacc atcaaatcca tttattcccg cgactcaaat ctctggcatc tttcgcacga
     1141 aggcggtctg ctcgaagacc cgtggaacga acccgaagag atcatgtatc agatgtccac
     1201 ttcgccggag aacgcgcccg aacaaggcga atatgtggag attgaattcg agagcggtaa
     1261 tccaattgca gtgaacggcg agaagatgtc gcctgccaag atcgtttcca cgctcaatgc
     1321 cattggtggc aatcacggca ttggtcgtgt tgacctcgtt gagaatcgcc tcgtaggcat
     1381 gaaatctcac ggcgtgtatg aaacccctgg tggcacaatc cttctgtatg cgcatcgcga
     1441 acttgagtct ctgattcttg accgcgagac attgcactac aaacaaacga ttgcagtcaa
     1501 atatgctgag atcacataca acggtctatg gttcacacca ctgcgcgaag cactcgatgc
     1561 tttcgtcaac tccacgcagg gacctgtcac cggcaccgtg cgcctcaaac tctacaaagg
     1621 caacatcatc agtgccgggc gcaaatccaa atttagcctc tatcgcgaag atttcgccac
     1681 ctttggtcag gaagatgttt acgaccagag tcatgccgaa ggtttcatcc gcttgtacgg
     1741 tctcagcatg aaagtccgcg cgatgaatgg tctgccggtt tccggtctcg atatgcccaa
     1801 gccagattat tcaaagttca aacgagatta ggagccattc atgaccctct ggggcggacg
     1861 cttttcccaa aaactagatg acctcgcatg ggcgctgaat gcttccctgc ctgtcgatca
     1921 acgcatggcg cttcaggatg tggaaggaag cattgcctgg gcggtagcga ttcataacgc
     1981 aggtattctt acggatgaag aacacgcctc gatctcaacc gggcttggta ccgtccgaaa
     2041 ggaattcgct tcgggtgaat ttatcttcca accttcggat gaagatattc acaccgcagt
     2101 cgaaaga
//
LOCUS       DAOQFT010000460         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35558_length_2106_cov_7.985373, whole genome shotgun sequence.
ACCESSION   DAOQFT010000460 DAOQFT010000000
VERSION     DAOQFT010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35558_length_2106_cov_7.985373"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="PLR93_10790"
     CDS             complement(<1..118)
                     /locus_tag="PLR93_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNH27499.1"
                     /translation="MHILVTNDDGVTAPGLLALAQEMRKLGKVTVFAPDKNWS"
     gene            complement(391..639)
                     /locus_tag="PLR93_10795"
     CDS             complement(391..639)
                     /locus_tag="PLR93_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27500.1"
                     /translation="MTESSPKIVFNPEGNGPGDACVPNISPAERKKRMDFGILQLVIT
                     FGILAAMLYFGADKLWRLPLFAMFSSGAVSIFQALDKT"
     gene            complement(779..1696)
                     /locus_tag="PLR93_10800"
     CDS             complement(779..1696)
                     /locus_tag="PLR93_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH27501.1"
                     /translation="MSTQTNSPAKQEYSVHPATRMGHVALTVANLENQIVFYEKAIGL
                     KLHWREGNKAGLGAGGADLLQLTEEPNLKRYRGGTGLYHFAIVFPNRRELAIAMARLF
                     ALKVPNSPTDHVMTKTTYLSDPEGNGIELYCESPEDGSWTIKDGNYEARWADGRWSDG
                     REALDVDALFTHLKEDDKLDAPVPAETRMGHVHLHVRDIPEAVDFYHGVLGFDVMGVS
                     KGIGMAFVSAGGYHHHIGLNTWNGRGAPPPPPDAVGLRFMTVDYPNPSVLAEVTARVE
                     AAGIPYNKTDDGYLLHDPSQNGVLLRSVG"
     gene            complement(1777..>2106)
                     /locus_tag="PLR93_10805"
     CDS             complement(1777..>2106)
                     /locus_tag="PLR93_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNH27502.1"
                     /translation="KLRTIPDYIVGSVHAITEAGEVLTASGTGSQISSYAYGAGKVIW
                     VVGTQKIVSDLNEGFRRIKEYSHPLEDARMQEAYGVNSSLNKILVLSRDMPGRITIVL
                     VKEELGF"
BASE COUNT          464 a          495 c          592 g          555 t
ORIGIN      
        1 cagaccagtt cttatccggc gcaaagacgg tcactttgcc gagtttgcgc atttcctgcg
       61 cgagtgcgag aagtccaggg gcagtgacgc cgtcatcatt ggttacgagg atatgcatag
      121 tctgatttcc gattttggat tgacgattgt gggtcgctct tacgattata cgccatgcga
      181 ttggcaaaga aaaacgctct gctttatgtg cagagcgttt tattgggttc atggaattag
      241 catgatcaac aaggttatgc ctagtccaac cagtgtccct ttgaggacga ctttcattgc
      301 ctggcgtttg atcttggcgt cttcggctga gtctttgacc cgttcatatt tatctgaggt
      361 gcggcgtaca ctacgcgcgg cgagggctac tcaggttttg tcgagggctt ggaagatgga
      421 aacggcgcca gaggagaaca tggcgaaaag cggcaaacgc cagagcttgt ccgcgccgaa
      481 gtagagcatg gcagcaagga tgccaaaggt aatcacaagc tggaggatgc cgaagtccat
      541 gcgtttcttg cgctcggcgg ggctgatgtt cggaacacag gcgtcgccgg gtccgttacc
      601 ttcgggattg aacacaattt taggtgatga ttcggtcata gtttattttc tctaaggcgt
      661 agagacgttt tgtgtgttgg gattcatacg ggcgttttgc cacgcttaat aaggaatgga
      721 ttagaaacaa aaacgccgta gagatttctc tacggcgttt ttgttgattt ctatagaatt
      781 atccaaccga gcggagcaag acgccattct gtgatgggtc atgcagcaag taaccatcgt
      841 cggttttgtt gtagggaatg ccagctgctt ctacgcgggc ggttacttcg gcgagcacag
      901 aaggattcgg gtagtccacg gtcatgaagc gcagtcccac cgcatcgggc ggaggaggag
      961 gcgcgccgcg tccgttccaa gtgttgagac cgatatggtg gtggtatccg cctgctgaga
     1021 cgaatgccat gccaatccct ttggaaacac ccatcacatc gaagccgagc acaccgtgat
     1081 aaaaatcgac agcttcggga atgtcgcgta catgcaagtg gacatgtccc atgcgggttt
     1141 cggcagggac gggggcatca agcttgtcat cttctttgag atgagtgaag agcgcatcga
     1201 cgtcgagtgc ttcgcgtcca tcgctccagc gtccgtctgc ccagcgggct tcatagttgc
     1261 cgtctttgat cgtccacgaa ccatcttcgg gcgattcgca atacagttcg atgccgttac
     1321 cttcagggtc actgaggtag gtggttttgg tcatgacatg gtcggtgggt gagttgggaa
     1381 ctttcaacgc aaacaaacgc gccatggcaa tcgcaagctc gcggcgattg ggaaatacga
     1441 tggcaaagtg atacaagccc gtgccgccgc gatagcgttt gagattcggt tcttcagtga
     1501 gttgaagtag atccgcgcct ccggctccga gaccggcttt attgccttcg cgccagtgca
     1561 gtttcaaccc gatggctttt tcgtagaaca cgatctgatt ctcaaggttg gcaacagtga
     1621 gagcgacgtg tcccattctg gtggcggggt gaaccgagta ttcttgtttt gcgggggagt
     1681 ttgtttgagt tgacatgtat acctttatct ttcgtccatg attctaagcc tatgtactag
     1741 atgacttaaa cacggaaata ctttctttgg ttcgggctaa aacccaagtt cttctttcac
     1801 cagaacaatg gtgatgcgcc ctggcatatc gcggcttaga accaatattt tgttgagcga
     1861 actgttgacg ccataggctt cttgcatgcg ggcatcttca agcggatgac tgtattcttt
     1921 gatgcggcgg aagccttcgt ttagatcgct aacaatcttt tgtgttccga ccacccaaat
     1981 cactttccct gcgccatagg cataggagga aatttggctg cctgtgcctg aagccgtaag
     2041 cacttcacct gcttctgtaa tcgcgtggac gctgccaaca atgtaatctg gaatcgtgcg
     2101 tagctt
//
LOCUS       DAOQFT010000461         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35640_length_2103_cov_5.277344, whole genome shotgun sequence.
ACCESSION   DAOQFT010000461 DAOQFT010000000
VERSION     DAOQFT010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35640_length_2103_cov_5.277344"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..657)
                     /locus_tag="PLR93_10810"
     CDS             complement(4..657)
                     /locus_tag="PLR93_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27503.1"
                     /translation="MNRSVSLVFVLMFLLSACGGAPANQTQAPAAALPSGSVLFQDDF
                     SQPTTGWDRLLVTEGVMDYYSGGYRMLVNSLQTNFWATPRRNFTDVRMEVDTGKLAGP
                     DENRVGLLCRYNGKDYYFFIMTSDGFYGIGLYKDGQATLLGQSEMLASSNINKGVAVN
                     HLRADCTGNILTFYINGFQVTQAQDTTLPSGDIGLIAGTFAEPGVDILFDNFVVLQP"
     gene            complement(671..2065)
                     /locus_tag="PLR93_10815"
     CDS             complement(671..2065)
                     /locus_tag="PLR93_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH27504.1"
                     /translation="MLLQRAKPYFIGLLLVIAIIIPFLVAGYTSLNRAENAYSTEQYR
                     LAAEEYAHTARILFWRKDLYGRAGISAAQGEDFEGAIAYFKQAKNLTEMGWVLFCQSY
                     AQLQDYPNTISTCKAGLQKYQSAPLYKILAFVYRDQKDWVAELDALKHQTQLDPNDAY
                     AAYRLGMLMALYAPEDSIPELTRASSLNPEVDSAVQTLRAALAVSSQQSDPSMQKVIV
                     GQAFGLVQDWELAKTAFEQAVQQNENNAEAWAWLGEAKQQTGQDGSAELDQALLLDHN
                     SVNVRALRGLYWSRKGKYSQMLAEYSLAARIEPENPRWQASLGEAYSKIGDLVAAMAA
                     YQRAVELDPTQADYWRLLAIFCAENNVHAEDVGIPAAQQAYTLSPNDPASLDALGYAY
                     LSTGRYASAELAFSQAIEISAQYYPAHIHLAMNYLAQGNRAAAFNALTYVRDADGAGV
                     YAETAKQLLDKYFQ"
BASE COUNT          567 a          522 c          484 g          530 t
ORIGIN      
        1 atattaaggc tgcaatacaa cgaagttatc aaaaagaata tcaacgccgg gttcggcgaa
       61 ggttccggca atcaagccaa tatcacccga aggcaaagtt gtatcttgag cttgcgtcac
      121 ctgaaaacca tttatataga aggttaagat attccccgtg caatcggctc gcagatgatt
      181 gaccgcaaca cctttgttga tattcgagga agcaagcatt tcagattgcc ccaacaaagt
      241 tgcctgacca tctttataca aaccgatccc gtaaaaacca tcgctggtca tgataaagaa
      301 atagtaatct ttgccgttat aacggcaaag cagacccaca cggttttcgt ctggacctgc
      361 taatttaccg gtgtccactt ccatgcggac atcggtgaaa ttacggcgtg gcgttgccca
      421 aaaatttgtc tgtaatgagt taacgagcat acgataaccg ccagagtaat aatccattac
      481 accttcggtc acgagcaaac gatcccagcc agttgttggc tgagaaaaat catcttgaaa
      541 aagaacagac cccgaaggca aggctgccgc tggagcctga gtttgatttg caggggcgcc
      601 gccgcatgca gaaagtaaaa acattagaac gaatactaaa gatacagatc gattcatgag
      661 cgtgatttta tcattgaaaa tatttgtcta ataactgctt ggcggtttcg gcatatacgc
      721 ctgcgccgtc ggcatcgcgc acatacgtca gtgcattaaa agccgcggca cgatttcctt
      781 gcgcaagata gttcatcgca aggtgaatat gagccggata atactgagcc gaaatttcaa
      841 ttgcttgtga aaaagccaat tctgcgctgg cataacgccc tgttgaaaga taagcatagc
      901 ccaaagcatc caaagaagcc gggtcgttgg gtgaaagggt ataggcttgt tgtgcggcag
      961 gaataccaac atcttctgca tgtacgttat tctctgcgca aaagatcgcc agcaagcgcc
     1021 aatagtcggc ttgcgtggga tcaagttcta ccgcacgttg atatgccgcc atagccgcca
     1081 ccaagtcccc tatttttgaa taggcttcac ccaagcttgc ctgccaacgc ggattctccg
     1141 gctcaatgcg cgccgcaagc gaatactctg ccaacatttg cgaatacttg cctttgcgac
     1201 tccagtacag tccgcgcaaa gctcgcacat tgaccgaatt atggtctagg agtaatgcct
     1261 gatcaagttc cgcgcttcca tcttgacctg tttgctgttt cgcctctccg agccatgccc
     1321 acgcctcagc gttgttttca ttttgttgaa ccgcttgctc aaatgctgtt tttgccaatt
     1381 cccaatcttg taccaagccg aatgcctgcc ccacgatcac cttctgcata gaaggatcgc
     1441 tttgctggct ggaaactgct aaagcggcgc gcaaggtttg gacagcagaa tctacttcgg
     1501 ggttaagaga ggaggcgcgt gtcagctccg ggatggaatc ttccggggcg tacaaagcca
     1561 tcagcatccc aagtcgatac gcggcatagg cgtcgtttgg gtctaattga gtttggtgct
     1621 taagtgcatc aagttctgcc acccaatctt tttgatctcg atacacaaac gccaaaattt
     1681 tatataacgg tgcagattga tatttctgaa gccccgcctt gcaagttgaa attgtgtttg
     1741 ggtaatcttg cagttgtgca tacgattggc aaaacaagac ccaccccatt tcagtgaggt
     1801 tcttggcttg tttaaaatat gcaatggcac cttcaaaatc ttcgccttgc gccgccgaaa
     1861 tacccgcacg cccatacaga tcctttcgcc agaacagtat gcgggcagta tgggcatatt
     1921 cttcagccgc aagccgatat tgctcggtag aatacgcatt ttcagcccgg tttaggctgg
     1981 tatagcctgc cacaaggaac ggaatgatga tggcaatgac aagcaaaaga cctataaagt
     2041 agggtttggc tctttgtagc agcataattt caattctacg ccataatccc taactggcag
     2101 tat
//
LOCUS       DAOQFT010000462         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35689_length_2101_cov_4.694037, whole genome shotgun sequence.
ACCESSION   DAOQFT010000462 DAOQFT010000000
VERSION     DAOQFT010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35689_length_2101_cov_4.694037"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PLR93_10820"
     CDS             <1..1066
                     /locus_tag="PLR93_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNH27505.1"
                     /translation="KERDTCYACVVKCKRVVEIKDGPYQVDPRYGGPEYETLGTFGSY
                     CGVDDLAAVSLANQICNEYAVDTIACGATIAFAMECYEKGIITREQTEGIDLKFGNAD
                     AMIQVLYKIVKGEGELGKILGMGSERAAKKWGNGAEDCLITVKGAEAPAHMPQAKRSL
                     ALVYAVNPFGADHQSSEHDPYYEEGVGDFNLDRLKQIGLASPQPAYSLTEEKVRFAYE
                     TEVFYSMLDSAELCQFVYGPTWTLYDGKDTVNMINSVTGWDMTLDELMEVGRRRLNLF
                     RVFNAREGLARNADKLPKKFFKQLAGTGPTAGFALTHEEVDTAIDTYYKLAGWTNDGV
                     PTRETLKKHDVEWAAEYLPA"
     gene            1179..>2101
                     /locus_tag="PLR93_10825"
     CDS             1179..>2101
                     /locus_tag="PLR93_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH27506.1"
                     /translation="MTINQLFQDNFPHMTPAWSRIFNFVADRAEGAYIYTDDGKKLLD
                     FTSGIGVTNTGHCHPKVVEAIREQAGLFLHAQANLVIHKPMLQLIEELRKIVPPSMDS
                     FYFANSGAEALENAVKIAKAATGRPNVIVFNGSFHGRTHATMALTTSKTGYRTGFAPL
                     LGGIYVSPFPYAFHLKMTEEQASQYALEQLEYLLASQTAPRDTAAILIESVLGEGGYI
                     LPPASFMKGLREICDKHGILLIFDEVQSGFGRTGKWFALEHFGVVPDIITAAKGIASG
                     MPLSAVFSRTDIMKKLDVGSIGGTYGGNAVA"
BASE COUNT          522 a          494 c          559 g          526 t
ORIGIN      
        1 aaaagaacgc gatacctgtt atgcatgtgt ggttaaatgc aagcgagtgg ttgaaatcaa
       61 ggatggacct tatcaggtcg acccgcgtta tggcggtccg gaatatgaaa cccttggtac
      121 attcggatcg tactgcggag tggatgacct tgctgctgtt tcactggcaa accagatctg
      181 taacgaatat gctgtagata ccattgcctg tggtgcgacc attgcatttg caatggaatg
      241 ctatgaaaaa ggcattatca ccagggagca aacggaaggc atcgatttga aatttggaaa
      301 tgctgacgca atgatccagg tgctgtataa gatcgtgaag ggtgaaggag agcttggcaa
      361 aatactcggt atggggtcgg agcgcgcagc caagaaatgg gggaatggtg ccgaagattg
      421 tctcatcaca gtcaaaggtg cggaagctcc agctcatatg ccacaagcca agcgttcgtt
      481 ggcgctcgtg tatgcggtca atccttttgg tgccgatcac caatcttcag agcatgatcc
      541 atattacgaa gaaggtgtgg gtgatttcaa cctggatcgc ctcaaacaaa tcgggcttgc
      601 ttcgccgcaa cctgcttatt cacttactga ggaaaaagtc cgctttgctt atgagactga
      661 ggtcttttac tcgatgctgg actcggcgga actttgtcag tttgtttatg gccccacgtg
      721 gacactgtac gacggcaagg atacggtcaa tatgatcaac tcggtgacgg gttgggacat
      781 gactctcgac gagttgatgg aagttggccg tcgccgcttg aatctcttcc gtgtgttcaa
      841 tgcgcgcgag ggactggctc gcaatgcgga taaactgccg aagaaattct tcaagcaact
      901 ggcaggcacc ggtccgacag caggtttcgc tctgactcac gaggaggttg atactgcgat
      961 tgacacctat tacaagctgg caggttggac gaacgatggc gttcccaccc gtgagactct
     1021 caagaaacac gatgtcgaat gggctgcgga atatcttcct gcatagtgtg tgaaatgtaa
     1081 cgcatgatga ccggggatgg agtgatcaaa tcattccatc ccctttttgg gcggcagtct
     1141 gttgcgtact acaaacaacg ttttacggga tacttcaaat gacaataaat caactctttc
     1201 aagataactt cccccacatg actcctgcgt ggagccgaat tttcaatttc gtagcggatc
     1261 gtgccgaggg cgcatacatt tataccgatg acggtaaaaa actgttggac tttaccagtg
     1321 gcatcggtgt gaccaacacc gggcattgtc accccaaagt agtggaagcg attcgggagc
     1381 aggcgggtct tttccttcac gcacaggcaa accttgtcat ccataagccg atgttgcaat
     1441 tgatcgagga acttcgtaag attgttccac cttccatgga tagtttctac tttgccaact
     1501 ctggtgcaga ggctctggag aatgccgtca agatcgccaa agctgccacc ggcaggccaa
     1561 atgtgatcgt cttcaatggt tccttccacg ggcgcacaca tgccaccatg gcactcacca
     1621 cttccaagac aggctatcgc accggttttg cgccattatt gggcggtatc tatgtttcgc
     1681 cattccccta cgcctttcat ctaaaaatga cggaggaaca agccagccaa tatgcgcttg
     1741 agcaattgga atacctgctt gcttctcaaa cagccccaag agatacggct gcgattctca
     1801 tcgagtcggt gttgggagaa ggcggttata ttttgccgcc agcttcattc atgaaaggat
     1861 tgcgcgagat ttgtgataag catggcatcc tgctcatttt cgatgaagtg caatcgggct
     1921 ttggccggac aggaaagtgg tttgcattgg aacatttcgg ggttgtgcca gatattatta
     1981 ccgctgccaa gggaatcgcc tctggtatgc ccctctcagc tgtgttcagc cgtacagaca
     2041 tcatgaagaa acttgatgtc ggctcgattg gcggaaccta tggcgggaat gcagtcgcat
     2101 g
//
LOCUS       DAOQFT010000463         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35713_length_2100_cov_7.659658, whole genome shotgun sequence.
ACCESSION   DAOQFT010000463 DAOQFT010000000
VERSION     DAOQFT010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35713_length_2100_cov_7.659658"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLR93_10830"
     CDS             <1..870
                     /locus_tag="PLR93_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNH27507.1"
                     /translation="FMHSRRALLYMPGDNWKMITKSVTLGVDSICMDMEDGTAINKKA
                     EARATIAKALQELDFGASEKLARINSVGSGWEKDDIEAVLPYHPDGIVIPKVESFEQV
                     EWASKIIEDAELKNGWKVNSIRILIGVETAKGILNLKEIAAHPRLDAIIFGGEDFAAS
                     IGATRTKDAIELLYARQAVIVACAAYDLQPIDIVTIDYKDLEALKVESEFGARLGFSG
                     KQVIHPNQVPVVQEAFTPSAEAITYARRIVETFEASQKEGKGAYSLDGKMIDMPLLKN
                     AQKVLARAKAAGK"
     gene            872..2002
                     /locus_tag="PLR93_10835"
     CDS             872..2002
                     /locus_tag="PLR93_10835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNH27508.1"
                     /translation="MENRIKELDGLRGIAVVLVMALHLFKRAAYFTEHPVLLGFTKLT
                     TVGWVGVDIFFTLSGFLITSILLKSKTDEHYFKNFYVRRALRIFPLYYAAIAFVLFFA
                     PKVEEEFTNQLSTALPIMLLYQQNWALLFKDFHITQYLGITWSLAIEEQFYFLWPFIV
                     YKLDRNTLIKVSIGYIAVSILGRVLGTLLWPNLGESSTFFYYTSFARFEEMLFGGLLA
                     TFLTYEGALEKVKRYALPLFLASFTVFVALHILSLPGDPHPEHASLPLTLGGYTTAAL
                     FTLGLIGIFVTRPPQNLFRRFFANPVLTFLGKYSYSIYLFHMTAALILLDVFWHSELR
                     GWKPYFLYPLSVYAVTVIIALLTWNLLEKHMLGLKKYFEYKS"
BASE COUNT          525 a          482 c          500 g          593 t
ORIGIN      
        1 tttatgcact cacgacgcgc actactttac atgcccggtg acaattggaa gatgatcacc
       61 aagtccgtta cgctgggcgt ggattccatt tgcatggata tggaagatgg cacagccatc
      121 aacaaaaaag cggaagcacg cgccaccatc gccaaggcac tgcaagagtt ggactttggc
      181 gcaagcgaaa aactggcgcg catcaactcg gtcggttcgg gctgggagaa ggacgatatc
      241 gaagccgtcc tcccctatca cccggatggc attgtgattc cgaaagtcga atcgtttgag
      301 caggtggaat gggcgagcaa gatcatcgaa gatgctgagt tgaagaacgg ctggaaagta
      361 aactcaattc ggattttgat cggcgtggaa acagcgaaag ggattttgaa tctcaaagag
      421 attgcggcac atccacgttt ggatgcgatt atcttcggcg gtgaggactt tgcggcatct
      481 atcggtgcaa cgcggacaaa agatgcaatt gagttgttgt atgcgcggca ggcggtcatt
      541 gtcgcttgtg cggcgtatga tcttcagccc attgatattg tcaccatcga ttacaaggac
      601 ttggaagcgc tcaaagttga atcggaattt ggagcaagac tcggcttcag cgggaaacag
      661 gtcattcacc cgaatcaggt tccggtggtg caagaggcgt tcactccatc tgcggaagcg
      721 atcacgtatg cgcggagaat cgtcgagacc tttgaagcca gtcaaaaaga aggcaaaggc
      781 gcatattcgc tggatggcaa gatgatcgat atgccgttat tgaagaatgc gcaaaaagtg
      841 ctggcacgcg caaaagcggc agggaaatag tatggaaaat cgtatcaaag agttggacgg
      901 tttacgcggc atcgctgtag tgttagtgat ggcgttacat cttttcaaac gtgccgcata
      961 cttcacagag caccctgttc tattagggtt tacaaaactt acaaccgtcg gctgggtagg
     1021 cgtggacata ttcttcacgc tctctggttt tctgatcaca tccattctgt tgaagtcgaa
     1081 gacggatgaa cactatttca agaatttcta tgtacgccgt gccttgcgaa ttttcccgct
     1141 ttattatgct gccattgcct ttgtactttt ctttgcgccc aaagtagaag aagagttcac
     1201 caatcagtta agcacagcat tgccaatcat gttgttgtat caacagaact gggcattgtt
     1261 attcaaagat tttcatatca ctcaatattt ggggattact tggtcgcttg ccattgaaga
     1321 acagttttat ttcttatggc cttttattgt ttataaacta gatcgaaaca ccttgatcaa
     1381 ggtcagcatt ggatacattg ctgtttccat tcttgggcgt gtactgggca cgcttttatg
     1441 gccaaacctt ggagaatctt caacattctt ttattacacc tcttttgcac gctttgaaga
     1501 aatgctcttc ggcggtttgt tggcaacctt cttaacgtat gaaggtgctt tggaaaaagt
     1561 caaacggtat gccttgccgc tatttctggc ttcatttaca gtttttgttg ccctacatat
     1621 tttgtctcta cccggtgacc ctcaccctga acatgctagc ctgcctctca cacttggcgg
     1681 atataccacc gcggctcttt ttaccctcgg cttgatcggc atcttcgtta ctcgcccacc
     1741 ccagaacctt ttccgccgct tcttcgccaa tccggtgctg accttcctcg gcaaatacag
     1801 ttattccata tatctcttcc acatgaccgc agcattgatc ttgttggatg tcttctggca
     1861 cagtgaatta cgcggctgga aaccatactt cctctatccg ctgtcggtct atgctgttac
     1921 ggtcatcatt gcgctgctga catggaattt gctcgaaaaa cacatgctgg ggttgaaaaa
     1981 gtattttgaa tacaagtctt aatgcaaaac tccgaggtca tgagaacctc ggagtttttt
     2041 atcctaatct ttccataaag acattatcgt cgtaatgctt ttgaatggca ttttgataga
//
LOCUS       DAOQFT010000464         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_35902_length_2094_cov_4.700343, whole genome shotgun sequence.
ACCESSION   DAOQFT010000464 DAOQFT010000000
VERSION     DAOQFT010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35902_length_2094_cov_4.700343"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2094
                     /locus_tag="PLR93_10840"
     CDS             <1..>2094
                     /locus_tag="PLR93_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH27509.1"
                     /translation="DLSTSPTEQAESKVNEPAPSQGQAGNLKFVPTDVKNSSWLTRFW
                     NWLSEPSADLVDVTERNIARLSTSFLLIIAVLEFVGGLARIPRSGLVNAFLGPIGYTL
                     IPTLVAYGLSKTKWYRAAIFLFSLTFSSLAYISMVLEGNTADISALILVYVPLSLIVA
                     STFLSSWAIFLLTGLNVGLFLLTTQMGVILTPDNFGAQTGIITTIGLVLIVLSNFRAR
                     TEQLRLNTLKTINRQLEDASVNLEKRVQDRTHDLELASEVGRIVTARVDNLYQLLSHA
                     VELIRSRFGLYYTQIYLTDRSENIIVLRAGTGDAGRQLLQRGHQLKINLESLNGRAAL
                     EKKAVIVSDTTTNPSFLPNPLLPLTRSEMSVPLLIGERVVGVLDMQSELPNALSANNL
                     TAFEALAGQLAIAIQNAALFDQVNLARAEVEEQAKVLSYSGWASFLDALEHNERIGFI
                     YNQDEVLPYTGDTIENTGLNALGASINVSGAEIGKIHLMDDPNRQWTEFESTIIHNTA
                     AQLSRHIENLRLLAQAEKYRRDAEEATKRLTRDGWEEYHRIHSSDQSGFEYDRNSVKG
                     ISDASDNSIQTTLAQPVVVRGEVVAKLEAAEAQTMATEEAKDLLAEVSRALSAHIENL
                     RLFEQVETSLSATEKLYDASRALTNASTEQEALDELVQRLDRTGLDRIVAAVKISNDP
                     ITAEVVSAWDVDGAEA"
BASE COUNT          553 a          495 c          492 g          554 t
ORIGIN      
        1 gatttaagca catctccaac ggagcaggct gaatcgaaag taaatgaacc tgcaccatct
       61 caggggcagg caggcaattt aaaatttgtg cccacagatg tgaaaaattc ttcgtggctg
      121 acacgttttt ggaactggtt atctgaacct tctgcagatc ttgtagatgt tactgaaaga
      181 aatattgcca gactttccac aagctttttg ttaatcatag ccgtccttga gtttgtgggt
      241 ggattggccc gcatcccgcg ctcgggtttg gtcaatgcat ttttaggtcc aattggttat
      301 acgctcattc caactctcgt agcatatgga ctctccaaga caaagtggta tcgtgcagcg
      361 atcttccttt tctctcttac attcagctcg cttgcctata tatccatggt attggaaggc
      421 aatactgcag atatcagtgc gttgatcctg gtttatgttc cattaagtct gatcgttgca
      481 agcacgtttc tatcctcctg ggcgatcttt ctattaacag gtttaaatgt tgggcttttc
      541 ctgcttacca ctcaaatggg cgttatcctg actcccgata attttggtgc acaaaccggc
      601 atcattacca ccattgggct tgtcttaatc gtcttatcca attttagagc acgaacagaa
      661 caactgcggc ttaacacatt gaaaacaatc aaccgtcaac ttgaagatgc aagtgtaaat
      721 ctggaaaagc gtgttcagga tcgcacgcac gacctcgaac ttgcatccga agttggacgc
      781 attgtcacgg ctagggtgga taatctatat caacttctct cccatgccgt ggagttgatt
      841 cgttcgcgct ttgggctgta ctatacccag atatacctca ctgaccggag tgaaaatatc
      901 attgtattac gtgctggaac aggcgatgcc gggcggcaat tattgcagcg tggacatcag
      961 cttaagatca atcttgaatc cttgaatggt cgggctgcgc tggaaaaaaa ggctgtcatc
     1021 gtatcagata ccacaacgaa tccttctttc ctgcctaacc ctctattacc tctgacccgc
     1081 tccgaaatgt cagttcctct cttgatcggc gagcgtgtgg tgggtgttct ggacatgcaa
     1141 agtgaacttc caaatgcact tagcgcaaac aacctgactg ctttcgaagc tctcgcaggc
     1201 caattggcca ttgccattca aaatgccgcg ctttttgatc aggttaatct ggcacgtgca
     1261 gaagtagagg aacaagcaaa agttctatcg tattccggct gggccagttt tctggatgcg
     1321 ctggaacaca atgaacggat tggttttatt tacaatcagg atgaagttct gccttacacc
     1381 ggagatacga ttgaaaacac cggcttgaat gcgttgggag cctccatcaa tgtgtctgga
     1441 gcagagatcg ggaaaatcca ccttatggat gatcccaacc gccaatggac tgagtttgaa
     1501 tcaaccatta ttcacaacac agctgcacaa ctttctcgcc acattgaaaa cctacggcta
     1561 ttagcccagg cagaaaagta ccgccgagac gcagaagaag cgaccaaacg cctaactcgt
     1621 gatggttggg aagagtatca tcgcattcat tcctcagacc agtctggctt tgaatacgat
     1681 cggaatagcg ttaaaggcat ttcggatgca agtgacaact caatccaaac aacattggca
     1741 cagcccgttg ttgttagagg tgaggttgtt gccaaattgg aagccgccga agcgcagacc
     1801 atggcaaccg aagaagccaa agacctgctg gcggaggttt ccagggcatt gagtgcccat
     1861 attgagaacc tgcgtctgtt tgaacaagtc gaaacttccc tctcggctac tgaaaaactg
     1921 tatgatgcca gccgtgctct tacaaatgct tcgacagaac aggaagcttt ggatgaatta
     1981 gttcagcgac ttgaccggac cgggctggat cgaatagtgg ctgcagttaa aatatccaat
     2041 gacccaataa cggctgaagt tgtctcagcc tgggatgtgg atggggcaga ggca
//
LOCUS       DAOQFT010000465         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_36043_length_2089_cov_6.034415, whole genome shotgun sequence.
ACCESSION   DAOQFT010000465 DAOQFT010000000
VERSION     DAOQFT010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36043_length_2089_cov_6.034415"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLR93_10845"
     CDS             <1..329
                     /locus_tag="PLR93_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH27510.1"
                     /translation="REWREAAENLGATQFQYWSKVAMPILLPPLLGATILLFGNAFGA
                     YATAYALTGGTLPIVTIQIGAQIRGDVLHNPGLGYAMAMGMVIIMAISITGYTWLQKI
                     SERWLK"
     gene            326..1156
                     /locus_tag="PLR93_10850"
     CDS             326..1156
                     /locus_tag="PLR93_10850"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27511.1"
                     /translation="MKKRSSRNFPFWSWVWFILGALYFILPLYATLDFSLQMERDVIG
                     FKAYEKAFEDKDFIETFTYSNILAFATIAVSIILIVPTAYWIRLRVPEARRAVEFITL
                     LPFVVPGIILVFGLIRIYSSPLTIPFTDIVLMQPLTTYKFGTNVLIVAGYMVLAMPYM
                     YRSVDTGMRTVDVATLTEAAQSLGASWFTIITRIILPNIRSALLSGALLTFAIVVGEV
                     TLASFLGVDAFGPYLFLLGQHRAYEPAALSFVSFLLTWAAMGLIQLFTGGQGQTAGAH
                     "
     gene            1176..>2089
                     /locus_tag="PLR93_10855"
     CDS             1176..>2089
                     /locus_tag="PLR93_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27512.1"
                     /translation="MAFLSISNVSKQYGDATVVDNFNLDIEKGEFVSFLGPSGCGKTT
                     TLRMVAGFEIPTMGKIILDGADITNKAPNQRNVGMIFQSYALFPNMTVAQNIGFGLRV
                     RKEPEETVKKRVDEMINLINLEKHAHKYPYQLSGGQQQRVSLARALANRPRVLLLDEP
                     LSALDAKIRVSLRTEIRSIQKSLGITAIFVTHDQEEALSISDRIVVMNVGVIEQVGTP
                     FEIYNFPQTQFVANFVGSLNTAKAEVTDPANGLLTVDGVQFVTASDIKSRKKGDAIRV
                     SIRPERFSFADVQKKDNVLDCTIENITFL"
BASE COUNT          471 a          498 c          524 g          596 t
ORIGIN      
        1 aacgtgaatg gcgtgaagct gccgaaaatt tgggcgcgac ccaatttcaa tattggtcaa
       61 aggttgccat gccgatatta cttccgccat tgttgggtgc aactattttg ttgttcggaa
      121 atgcgtttgg tgcgtatgcc acagcctatg cattgacggg tggcaccctg ccaattgtga
      181 cgatccagat cggggcgcag atccgcggcg acgttcttca taatcccggt ttggggtacg
      241 ccatggcgat gggtatggtt atcatcatgg cgatttccat cacggggtat acctggctac
      301 agaaaatatc tgaaaggtgg ttgaaatgaa aaaacgatca tcacgaaact ttcctttttg
      361 gagctgggtt tggtttattt taggcgcact gtattttatt ttgcccctat atgccactct
      421 tgatttttcc cttcaaatgg agcgcgatgt cattggtttc aaagcgtatg agaaagcctt
      481 tgaagataaa gacttcattg agactttcac atattccaac attttggcgt ttgcaaccat
      541 tgctgtcagt attattctga tcgtcccaac cgcatactgg attcgcttgc gtgttccaga
      601 agcgcgccgt gctgtggaat tcatcacctt gttgccgttt gtggtgcccg gcattatctt
      661 ggtgtttggg ttgatccgaa tttacagttc acctttgacg attccattta ccgatattgt
      721 tttgatgcaa cctctcacga cctataagtt cggcaccaac gtgttgatcg tggctggcta
      781 tatggtgctt gccatgccat atatgtaccg ttcggtggat acgggcatgc gtactgtgga
      841 tgtagcaact ttgacagaag cggctcaaag cctcggcgcg agctggttca ccatcatcac
      901 acggatcatt ctcccgaaca ttcgctcggc gctccttagt ggtgccttgt taaccttcgc
      961 cattgtagtg ggcgaagtga ccctcgcttc cttcctcggc gtggatgcgt tcggtccata
     1021 tctcttcctg ttggggcagc atcgtgcata tgaacctgcc gccctttcat ttgtaagctt
     1081 cctgttgaca tgggcggcaa tggggttgat ccaattattc actggcggac aaggtcagac
     1141 cgccggggca cactaatctt ttgaggagaa tggcaatggc atttctttcc atttcaaatg
     1201 tttcaaagca atacggcgat gccacggtgg tggataattt caatctcgat atcgagaagg
     1261 gtgaatttgt atcttttctt ggtccgtctg gctgtggaaa gaccacaacc ttacgcatgg
     1321 tggcagggtt tgagatccca accatgggga agatcattct ggatggcgcg gatattacca
     1381 ataaggcacc caatcaacgc aatgtgggta tgatcttcca atcctacgcc ctattcccga
     1441 atatgaccgt tgcccagaac attgggtttg gtcttcgagt tcgcaaggag ccggaagaaa
     1501 ccgtgaaaaa gcgtgtggac gagatgatca acctgatcaa tctcgaaaag catgcccaca
     1561 aatatcccta tcagctttcg ggcgggcagc aacagcgtgt ttctttggct cgtgcattag
     1621 ccaaccgtcc gcgtgtgctt ttgttggacg agccgctttc agctttggat gcgaaaatcc
     1681 gcgtttcatt gcgtacggag atccgctcga ttcaaaagtc actgggcatt accgccattt
     1741 tcgtaaccca tgaccaggaa gaagcgctct ctatatctga ccgtatcgtg gttatgaatg
     1801 tgggtgtgat cgagcaggtt ggtacgccct ttgagattta caacttccct caaacccagt
     1861 tcgtggcgaa cttcgttggc tccctcaaca ctgccaaagc agaagtgaca gaccctgcga
     1921 atggactttt gactgtggat ggcgtccaat ttgtgactgc ctctgatatc aaatcccgca
     1981 agaaggggga tgcgatccgt gtttccatcc gcccggaacg cttcagcttt gcggatgttc
     2041 aaaagaaaga taatgttctc gactgtacga ttgagaatat taccttcct
//
LOCUS       DAOQFT010000466         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_36225_length_2083_cov_6.631164, whole genome shotgun sequence.
ACCESSION   DAOQFT010000466 DAOQFT010000000
VERSION     DAOQFT010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36225_length_2083_cov_6.631164"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLR93_10860"
     CDS             <1..497
                     /locus_tag="PLR93_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27513.1"
                     /translation="PAISHGHPGRGEVRPRLATKEDVHSLWTNVDVIDCFATDHAPHT
                     LAEKDGENPPPGFPGLETLLPLLLQGVQDGRLTIDQIVEKLYTNPKRIFNLPEQPETW
                     VEVDEDVTYEIKASESFTRCGWTPFEGWKVKGKVRKVVLRGETAFEDGKVFAEAGSGK
                     NVRK"
     gene            complement(537..1712)
                     /locus_tag="PLR93_10865"
     CDS             complement(537..1712)
                     /locus_tag="PLR93_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNH27514.1"
                     /translation="MSKQTFKWDDPFLLNDQLTDEERMIRDTARKYCQDKLMPRILEA
                     NRHEVFHREIMTEMGNMGFLGSTIPEEYGGAGLNHVAYGLIAREVERVDSGYRSAMSV
                     QSSLVMYPIFTYGTEEQRKKYLPKLASGEWVGCFGLTEPNHGSDPASMEAKAKKVDGG
                     FILHGNKMWITNSPIADVFVVWAKLDGEIRGFILEKGMKGLSAPKIEGKFSLRASATG
                     EIVMDEVFVPAENIFPEVKGLKGPFGCLNKARYGIAWGALGAAEFCWHAARQYTLDRP
                     QFGRPLAANQLMQLKLANMMTEITLGLQGALRVGRLIDEDKVSPEMISLIKRNSCGKA
                     LEIARTSRDMHGGNGISDEYHIIRHVMNLEAVNTYEGTHDIHALILGRAQTGIQAFS"
     gene            complement(1871..>2083)
                     /locus_tag="PLR93_10870"
     CDS             complement(1871..>2083)
                     /locus_tag="PLR93_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HNH27515.1"
                     /translation="YFEQVQNDMNFYWSKDEVLQRLDEKMTSAFNSVFDRSEKEKVYM
                     RDAAYMVAIDRVVKAMELRGWLTGSA"
BASE COUNT          531 a          535 c          504 g          513 t
ORIGIN      
        1 tccccgccat ttcacacgga caccccggca gaggcgaagt ccgcccgcgc ttggctacca
       61 aagaagatgt ccacagcctg tggacaaatg tggatgtgat cgattgtttc gccactgacc
      121 acgctccaca cactttggct gaaaaagatg gcgagaatcc gccacccgga ttccccggtt
      181 tggagacttt gctccccttg ttgttacaag gcgtgcaaga tggaagatta acaatcgacc
      241 aaattgtgga aaaactttac accaacccca aacgcatttt caatctgccg gaacagcccg
      301 aaacctgggt ggaggtggat gaagatgtaa cttatgaaat caaagccagc gagagtttta
      361 cccgctgcgg ctggactccc tttgaaggat ggaaggtaaa aggaaaagtt cgtaaagtgg
      421 tgctaagagg agagaccgct tttgaagatg gcaaggtttt cgcagaagcc ggctctggca
      481 agaatgtaag aaaataaaaa agaatggaca ggtttctcct gtccattctt ttttatctaa
      541 gaaaaggctt gtattcctgt ttgcgctcta cctaagatca aggcatgaat atcgtgtgtg
      601 ccttcatagg tgttgaccgc ttccaaattc atcacatgcc gaatgatgtg atattcatct
      661 gagataccgt tgccgccgtg catatcgcgt gacgtgcggg caatctccaa ggctttgccg
      721 caggagttgc gtttgataag cgagatcatt tcaggcgaaa ctttgtcttc gtcgatgagg
      781 cgcccaactc gtaacgcacc ttgtaatccc aacgtaattt ccgtcatcat attggcgagt
      841 ttcaattgca taagttgatt cgccgccagc ggacgcccga actgcggacg gtctaaggtg
      901 tattgtcttg cggcatgcca gcaaaattct gccgcgccaa gggctcccca ggcaatgcca
      961 tagcgcgcct tgttcaaaca cccgaacgga cctttcagtc ccttcacttc ggggaagata
     1021 ttctctgccg ggacgaatac ttcgtccatc acgatctcgc cggtggcact cgcccgcaga
     1081 ctgaacttcc cttcgatctt gggagcagag agtcctttca tgcctttttc caagataaag
     1141 ccgcggatct cgccatcgag ttttgcccaa acgacaaaga catccgcaat tggtgagttg
     1201 gtaatccaca tcttattgcc atgcagaata aaaccgccat ctactttttt ggctttggct
     1261 tccatactgg cggggtcact gccgtgattg ggttcagtca agccgaaaca gccaacccat
     1321 tctccactgg cgagttttgg cagatatttc tttctctgtt cttcggtgcc ataagtaaaa
     1381 atgggataca tcaccagaga tgactgtacg ctcatcgcag aacgatagcc gctatccacg
     1441 cgttccactt cgcgggcgat aagcccataa gcaacatggt tcaaaccagc accgccatat
     1501 tcttcaggaa tggtcgaacc gaggaagccc atgttgccca tctcggtcat aatttcgcga
     1561 tggaaaactt catgtcgatt cgcttcgagg atgcgcggca tgagcttatc ctgacaatac
     1621 ttgcgcgccg tgtcacggat catgcgttct tcgtcggtca attggtcatt gagcagaaag
     1681 ggatcgtccc atttgaaagt ttgtttggac atgaggacct cggggattag ggatagagtg
     1741 attgtatcaa aagcaagggg tagaaaactg tgtcagaaaa cagaagcaag catcggctaa
     1801 gaatgaagcg ttaatcaaaa agaggatgca ccctttcagg cacatcctct tatacatcac
     1861 atcgtactaa ctacgcagaa cccgtcaacc aaccacggag ttccatcgcc ttgaccacgc
     1921 ggtcgatggc gaccatgtaa gccgcatcgc gcatgtagac cttttccttt tcgctgcgat
     1981 cgaacacgct gttgaaggca gaggtcatct tctcatcgag gcgctgcagg acttcatcct
     2041 tgctccagta gaagttcata tcgttctgaa cctgttcgaa gta
//
LOCUS       DAOQFT010000467         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_36529_length_2073_cov_7.364718, whole genome shotgun sequence.
ACCESSION   DAOQFT010000467 DAOQFT010000000
VERSION     DAOQFT010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36529_length_2073_cov_7.364718"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="tal"
                     /locus_tag="PLR93_10875"
     CDS             complement(<1..684)
                     /gene="tal"
                     /locus_tag="PLR93_10875"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNH27516.1"
                     /translation="MSESIKKLTALGQSLWYDNIQRKLLVNGELKAMINRGDIRGVTS
                     NPTIFQNAIAKTNDYDAALVPLAWAGWDAEKIFWQLAVEDIQEACDLFRPLYDESKGG
                     DGYVSIEVSPFLARDTEGTIKQAQELWERVNRPNLMVKIPATKEGVPAIRASIAAGIN
                     VNVTLIFSLSRYAEVMNAYLEGIEERVGKDLPVQHVASVASFFVSRVDTKVDPKLPED
                     SPLRGKAAVA"
     gene            complement(741..1199)
                     /gene="rpiB"
                     /locus_tag="PLR93_10880"
     CDS             complement(741..1199)
                     /gene="rpiB"
                     /locus_tag="PLR93_10880"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNH27517.1"
                     /translation="MKVAVGCDHGGFPLKDVVLEAVKAMGHEVIDVGTFNAEAVDFPD
                     FTKKVGEKVQSGEAERGILICGSGIGAAIAANKMKGIFASICHDTYSAAQGVEHDAMN
                     VLCMGGRVIGPELVKVIVPAFLNAEYLGDKTGGERFARRVGKIRKMEENQ"
     gene            complement(1279..>2073)
                     /locus_tag="PLR93_10885"
     CDS             complement(1279..>2073)
                     /locus_tag="PLR93_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNH27518.1"
                     /translation="GSPAFQKDSPAGRNFHFGVREHGMGSVLNGMAIFGGVIPYGATF
                     LVFADYMRASVRLAALSHYPTIFIFTHDSVGLGEDGPTHQPVEHLASLRIIPNLVVLR
                     PADANETAQAWKAAIARRDGPTVLALTRQNVPTMDGPALVEKGAYVLKDFGTPEIILM
                     ASGSEVGLVMDAAQKLADEGKGVRVVSFPSWELFEKQDEAYRESVLPKSIQKRLAVEA
                     GSSIGWERYAKSVIGIDRFGASAPAKTIFEKLGFTVENVVAKAKEL"
BASE COUNT          545 a          582 c          453 g          493 t
ORIGIN      
        1 ggcaacagca gccttaccgc gcagtggaga atcttcggga agtttcggat cgacctttgt
       61 atcgacacgg gagacaaaga acgaagcgac tgaagcgaca tgctgaacgg gaagatcttt
      121 gcccacacgt tcttcaatgc cttcgaggta agcgttcatc acttcggcat agcgcgagag
      181 ggaaaagatc aaggtaacgt tgacgttaat ccccgccgca atgctggcac gaatagcagg
      241 cacgccttct ttagtagccg gaattttcac catcaggttc gggcgattga cacgttccca
      301 aagttcttga gcctgcttga tcgttccttc ggtgtcacgc gccaaaaacg ggctgacctc
      361 aatgctcaca tagccatcgc cgcctttgct ttcatcatac aacgggcgga acaaatcaca
      421 agcttcttga atatcttcta ccgcaagttg ccagaaaatc ttctcggcat cccaacctgc
      481 ccacgccagt ggaacgagag ccgcgtcata atcattcgtt tttgcaatgg cattttggaa
      541 aatagtcggg ttggaggtca caccgcgaat atcgccacga ttgatcatag ctttgagttc
      601 accgttgacc aaaagtttac gttggatgtt atcgtaccaa agcgattgcc cgagggcagt
      661 taatttctta attgattcag acataataaa aaatgctcct taaattaatt aaatagtaac
      721 tggtaattac caattactat ttactggttc tcttccattt tcctaatctt tcccacccgg
      781 cgcgcaaagc gttcaccacc cgttttgtcc cctaaatatt ctgcattcaa aaatgcaggc
      841 acaataactt ttaccagttc cggtccgatc acacgcccgc ccatgcaaag cacattcatg
      901 gcatcgtgct ccacgccttg tgcggctgaa tacgtgtcgt ggcagatcga agcgaaaatg
      961 cctttcattt tattggcagc aatcgcggca ccaatgcccg aaccgcaaat caaaattccg
     1021 cgctcggctt cgccgctttg cactttctca ccaacttttt tcgtgaaatc tggaaagtcc
     1081 acagcttcag cattgaacgt ccccacatcg atcacttcat gtcccatcgc cttcacagct
     1141 tcaagaacaa catccttaag cggaaatccg ccgtggtcac aacctactgc aactttcata
     1201 attctccaaa agtaagggcg acccatcttg gcaaagtcag gtctctgacc attccgcgcg
     1261 ggtcgcccct acgatccatt aaagttcttt cgccttcgct acaacattct caacagtaaa
     1321 gcccagcttt tcaaagatcg tcttcgccgg agcagatgca ccgaagcggt caataccaat
     1381 gacggacttc gcgtatcgtt cccagccgat acttgatccc gcttcgacag cgagtctctt
     1441 ctgaatgctt ttcggcaaca cagactcgcg ataggcttca tcctgtttct cgaacaattc
     1501 ccaactcggg aaggacacca cacgtacccc cttgccttcg tccgctagtt tttgtgcggc
     1561 atccattacc agcccaactt ctgagccaga tgccatgagg ataatttcag gggtgccaaa
     1621 gtctttcaag acataggcgc ccttttcaac caatgcagga ccatccattg taggaacatt
     1681 ttggcgggtc aatgccaaga cagtaggacc atcacggcga gcaatggcag ctttccacgc
     1741 ttgagccgtc tcattggcat ccgctgggcg aagcacaaca agattcggaa taatacgcaa
     1801 agaagccaaa tgctccacag gttgatgtgt cggtccatct tcgcccaaac cgacagagtc
     1861 gtgagtgaaa atgaaaatgg tcgggtaatg cgaaagagcc gctaaccgca cagaggcacg
     1921 catgtaatcg gcaaagacca ggaaggtcgc gccgtacgga atcacgccac cgaaaattgc
     1981 cataccattt aggacagacc ccatcccatg ttcgcgcaca ccgaagtgga agttacgtcc
     2041 agccggagag tctttttgga aagcaggcga acc
//
LOCUS       DAOQFT010000468         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_36621_length_2070_cov_7.588586, whole genome shotgun sequence.
ACCESSION   DAOQFT010000468 DAOQFT010000000
VERSION     DAOQFT010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36621_length_2070_cov_7.588586"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1478
                     /locus_tag="PLR93_10890"
     CDS             27..1478
                     /locus_tag="PLR93_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNH27519.1"
                     /translation="MEQKAGAQIGKRAFIQSLVILFVLMMVAGILTLAIPSGQYTRIE
                     VDGRETIDPNSFQLTERPNYPIWRWFIAPIEVLGSDSGLTVIVIIVVLFFAGGAFAVM
                     DKTGTLRAFIGRIVRRYSGRKYTLLWMVSLSFMFLGATLGTFEEVVLLVPVMIALSYS
                     LGWDALVGLGMSILATNMGFSAAISNPYTLGVSQQLAGLPLFSGAWYRIIIFAVIYFI
                     FMWFLSSYAKKIDKDPTSSLVHKEDNAEREKYKSMEDASFTEDNPKQNRAMNFFGFFL
                     IFIFVVMLSGPFVPAISDYALPIVGILFLIGGLGAGFISGAGGKTVWQGLIEGWGGLA
                     PSVPLILMAASIRFIIDSGGVTDTILHAAADPFKQLGAFPAALVVYALALGIEFFIAS
                     GSAKAFLMIPIVLPLADLIGVTRQTAVLAYIFGDGFSNLAYPTNPVLLISLGLTVVSY
                     PKWLRWTAKLWLWVILATVTFLGIAVAIHFGPF"
     gene            complement(1520..>2070)
                     /locus_tag="PLR93_10895"
     CDS             complement(1520..>2070)
                     /locus_tag="PLR93_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH27520.1"
                     /translation="AISKRLIDLMHGEIGFETLEGKGSTFWFSLPLTMINETTEVIAE
                     KQMPRQKDHLPFVVTKPALIVEDNLLNRDLISLQLRELGLNASYATNGKEAVELLKFQ
                     PDEYSIVLMDMNMPVMDGPAATKLIRENEAGTTKHVPIIAVTANVMTGTREFCLQAGM
                     DDYLSKPITLQSMREVLEKWLV"
BASE COUNT          471 a          506 c          454 g          639 t
ORIGIN      
        1 tctaactttt cccaaggagc caaaccatgg aacaaaaagc tggtgctcag atcggtaaac
       61 gtgcttttat tcaatcgctg gtgatcttgt tcgttttaat gatggtcgca ggtatattga
      121 ccctcgccat cccatcgggg caatatacgc ggattgaagt agacgggcgc gagacaatcg
      181 acccaaattc atttcaactc acagaacgcc caaactaccc tatctggcgt tggttcattg
      241 ctccaatcga agtccttggc agtgacagcg gtttgaccgt catcgtcatt atcgttgtgc
      301 tgttctttgc cggcggcgct ttcgctgtga tggataagac cggaaccttg cgtgctttta
      361 tcggtcgcat tgtccgcaga tatagtggac gaaaatatac cttgctatgg atggtctctc
      421 tatctttcat gttcctcggc gcaaccttgg gaacatttga agaagttgtg ctattagtcc
      481 ccgttatgat cgcactatcc tattcgcttg gctgggatgc gttggtaggg ttgggcatgt
      541 ccattctcgc aacgaatatg ggattttcgg ctgcaatttc caatccgtat acgcttggcg
      601 tttctcaaca acttgcaggg ttacctcttt tctcgggtgc ctggtatcgc attatcatct
      661 ttgcagtaat ttactttatc ttcatgtggt ttttgtcttc ttacgcaaaa aagatcgaca
      721 aagaccctac ctcctctttg gtgcataaag aagacaatgc cgagcgcgag aaatataaga
      781 gcatggaaga tgcctcgttt actgaagata accccaaaca aaaccgcgcc atgaatttct
      841 tcgggttctt cctgatcttc atttttgttg tcatgctttc aggtccgttc gtgcctgcga
      901 tctcagatta tgctttgccc attgtgggta ttcttttctt gataggcgga ttaggtgctg
      961 gctttatctc tggtgcgggc ggtaaaaccg tctggcaggg gctgatcgaa ggttggggtg
     1021 ggctcgctcc ttccgtccca ctcattttga tggcagccag tatccgtttc attatcgata
     1081 gcggcggtgt gaccgatacc attctgcatg cagccgcaga cccatttaaa cagcttggtg
     1141 cttttcctgc cgccttagtt gtttatgcac ttgcattagg gattgaattc ttcatcgcct
     1201 ccggctccgc taaagcattc ttgatgatcc ccattgttct ccctctcgca gacttgatcg
     1261 gtgtcacccg tcagactgcg gttttggcat atatctttgg cgacggcttc tcaaaccttg
     1321 cctatcccac caacccggtc ttgctcatta gtcttggtct caccgtagta agttacccca
     1381 aatggctacg ttggacagca aaactgtggc tctgggtaat tctagcaact gtgacatttt
     1441 tagggattgc tgttgcaatt catttcggtc cgttctaaag catgaaagag cctgcacatt
     1501 ttgtgcaggc tcttttatat caaacaagcc atttttctaa gacttcacgc atagactgta
     1561 acgtgatcgg tttgctcagg taatcatcca taccagcttg caaacaaaat tcacgggtgc
     1621 ctgtcatcac atttgcagta acagcaataa ttggtacatg ctttgttgtc cccgcctcat
     1681 tctcgcggat caactttgta gcagccggtc catccataac aggcatattc atatccatca
     1741 gaacgataga atattcatcg ggctggaatt tcaataactc gaccgcttcc tttccattgg
     1801 tagcataact ggcattcaag cctaattctc gtaattgaag tgaaatcaag tctcgattaa
     1861 gcaagttatc ttctacaatc agcgctggtt tcgtcacgac aaatggcaaa tggtcttttt
     1921 gccgaggcat ctgtttttct gcaatcactt cggtcgtttc atttatcatg gttaatggca
     1981 agctaaacca gaaggtggaa cccttccctt ccaatgtctc aaaaccgatt tctccatgca
     2041 tgagatcgat caagcgtttt gaaattgcca
//
LOCUS       DAOQFT010000469         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_36668_length_2068_cov_6.116741, whole genome shotgun sequence.
ACCESSION   DAOQFT010000469 DAOQFT010000000
VERSION     DAOQFT010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36668_length_2068_cov_6.116741"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLR93_10900"
     CDS             complement(<1..710)
                     /locus_tag="PLR93_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNH27521.1"
                     /translation="MLTKILIANRGEIAIRIMRACRELGIKTVAVYSEADKNAQHVQF
                     ADEAILLGGAAPKESYLNVDALLRATRDSQADAIHPGYGFLSENASFAAAVESANLTF
                     IGPSADSIRAMGDKAESKILMKKAGVPTVPGYEGLESYDDFKNAAKEIGYPVLVKAAA
                     GGGGKGMRVVNAESELNESIELARREALNAFGDERLLIEKYLAEAHHIEIQVFGDKHG
                     NLVHLFERECSVQRRHQK"
     gene            complement(700..1686)
                     /locus_tag="PLR93_10905"
     CDS             complement(700..1686)
                     /locus_tag="PLR93_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH27522.1"
                     /translation="MKILIIGGTRFIGRHLVTSARARGHEVTLFNRGKSNPGLFRRIQ
                     NIRGDREKDLDQLSGQWDAVIDTCGYLPRVVRLSAEALKEKVSRYVFISSVSVYANFS
                     KVGIKESDAVGTLADESVEEITGETYGPLKALCEKAVQDVYGARSLIIRPGLIVGPHD
                     PTDRFTYWPLRIAKGGDVLTPDRPDAMAQFIDARDLADFIIRLIDQNVSGTFNAVGYP
                     VTLNTIFETCKRISKSNANFKWAPIDFLEKNNVAPWSDMPIWVPEVGEDAGVSQVDVS
                     KAVAAGLTFSPLTDTVKAIYDWEFERPAEYEMKAGLKPEREAELLKLLNHAH"
     gene            complement(1683..>2068)
                     /locus_tag="PLR93_10910"
     CDS             complement(1683..>2068)
                     /locus_tag="PLR93_10910"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HNH27523.1"
                     /translation="ANEAPIVGTIQIILDGRYALFLYQSSIEGEAQHGMFTFGYNTQL
                     DRYETSWVDSFHTNTSIMFCTGNEIENGFSVLGSYPDPTGGPDWGWRTDVQMNGDELI
                     VTAYNILPEGLEAKATEIILKKVNA"
BASE COUNT          486 a          514 c          541 g          527 t
ORIGIN      
        1 atcttttggt gacgtctctg caccgagcat tcacgttcga agagatgcac gaggttgccg
       61 tgtttgtcgc caaagacttg aatttcaata tgatgtgctt cagcgagata tttttcaatg
      121 agcaggcgtt cgtcaccgaa agcattgagg gcttcgcggc gtgcgagttc aatagactca
      181 ttcaactcgc tttcggcatt gaccacgcgc atgcctttcc cgcctccgcc cgctgcggct
      241 ttgacgagga cagggtagcc aatttctttg gcggcgtttt tgaagtcgtc gtaactttct
      301 aagccttcgt aacctggaac agtgggcaca ccggctttct tcatcaatat ctttgattca
      361 gctttatcgc ccatggcacg gatcgagtcg gctgagggac cgataaaggt taggttcgct
      421 gattccactg cggcggcgaa agaggcgttc tctgagagga agccatagcc tgggtgaatg
      481 gcatcggctt gagagtcacg agtggctcgc agtaaggcat ccacattcaa ataagattct
      541 ttcggtgctg cccccccaag caagatggct tcatcggcga attgcacgtg ttgtgcgttc
      601 ttatccgctt cggaatagac ggcaactgtt ttgatgccaa gctcacgaca ggcacgcatg
      661 atacgaatgg cgatctcgcc gcgattggcg ataaggattt tagtgagcat ggttgagtaa
      721 tttaagtaac tcggcttcac gctcaggctt caagcctgct ttcatttcat actctgcggg
      781 gcgttcaaac tcccagtcgt aaatggcttt gacggtatct gtgagcggcg agaaggtaag
      841 cccggctgct accgctttcg aaacatccac ctgcgaaacc ccggcatctt cgccgacctc
      901 tggaacccag atgggcatat cgctccatgg ggcaacgttg ttcttttcaa gaaaatctat
      961 aggcgcccat ttgaaattgg cgttgctctt actaatccgt ttacaggttt caaaaatagt
     1021 attgagcgtg acgggatacc caacagcatt gaaggtgccc gaaacatttt ggtcaatgag
     1081 ccggatgatg aaatcagcga ggtcgcgcgc atcaataaat tgagccattg catcggggcg
     1141 gtctggagtg agcacatctc cacctttggc gatgcgtaaa ggccaatagg tgaaacggtc
     1201 agtggggtca tgcggaccca caatgagccc agggcgaata atcagtgagc gggcaccata
     1261 tacatcttgc actgcttttt cgcacaaagc ttttagagga ccataggttt cgccagtgat
     1321 ctcttccacg ctttcatcgg caagggtgcc aacggcatcg ctttctttga tgccaacctt
     1381 gctaaagttc gcatacaccg aaacgctgga aataaataca taacggctga ccttttcttt
     1441 cagcgcttca gcagagaggc gcacgacgcg cggcaggtag ccgcaggtat caatgaccgc
     1501 atcccactgc cccgataact gatccaaatc tttttctcga tctccccgaa tgttttggat
     1561 tcttcgaaaa agacccgggt tgctttttcc gcgattgaag agcgtaactt catgtccgcg
     1621 ggcacgggca gacgtgacga gatgccttcc gataaaacga gtcccgccga taatgaggat
     1681 cttcatgcat tcaccttttt aagaatgatt tcagtggctt tggcttcaag cccttctggc
     1741 aaaatattat aggcagtgac gattaattcg tcaccattca tctgtacatc ggtgcgccac
     1801 ccccaatcgg gtccgccagt ggggtcggga taactgccaa gcacagagaa tccattctcg
     1861 atttcgttac cggtgcagaa catgatggat gtattggtgt ggaaggagtc gacccaactg
     1921 gtctcgtagc ggtcgagttg ggtgttgtag ccgaaggtga acatgccatg ctgggcttca
     1981 ccttcaatgg aagattgata taggaagagc gcgtaacgtc catcgaggat gatttgaatg
     2041 gttccaacga tgggagcttc gttagcca
//
LOCUS       DAOQFT010000470         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_36726_length_2066_cov_7.168076, whole genome shotgun sequence.
ACCESSION   DAOQFT010000470 DAOQFT010000000
VERSION     DAOQFT010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36726_length_2066_cov_7.168076"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..703)
                     /locus_tag="PLR93_10915"
     CDS             complement(2..703)
                     /locus_tag="PLR93_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27524.1"
                     /translation="MNRLVLLAGLIITLVGCTTKSTPPPPTPIPATATQSVPTPTPVP
                     PTPTLVEIPVQVNAEVANCRFGPGTVYQTMNEVRGGRLLNAVGRDAASTWWQVEDPIN
                     PGGYCWVAAEVTTLQGTAEQLPIVDAPFVSVTKVDLRVEPNRIVVNCNDFPQTVFFEA
                     NITANGPTLLNWQWEASTGVTSDVGTLIFEESGTQVINEFYRINAPNEYWVKLKILSP
                     NQMEEQVTFPVSCNP"
     gene            938..1507
                     /locus_tag="PLR93_10920"
     CDS             938..1507
                     /locus_tag="PLR93_10920"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNH27525.1"
                     /translation="MSSLDKAVQTQLENIQKKTGKSLAELAAIAQKSGLTKHGELRDM
                     FKEKLGLGHGDANALVHAVFKSDGTRAAEGKSSDAVLDEIYVGAKASQRPIHEALMKQ
                     INKFGEFEVVPKKGYVSLRRKKQFAMIGPKTNTRFEVGINAKEMKKNPRLVEQPKGSM
                     CNYIVALTDASEVDAELIGWIKLAYEGAD"
     gene            1507..1965
                     /locus_tag="PLR93_10925"
     CDS             1507..1965
                     /locus_tag="PLR93_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH27526.1"
                     /translation="MSEDFRKNLLNFMTETFESPMGIYLDKNTALFQTLETVSAAEAS
                     IPVGGKCATLAAQVAHVTYYLDVLERYVVHNDTSPADWGEVWRTVSGVTTQEWETSKA
                     KLRSAYQRMVELFKTNPVWNETTMGGAMAIVVHSAYHLGEIRQALCTLKK"
BASE COUNT          543 a          432 c          580 g          511 t
ORIGIN      
        1 tttaaggatt acagctaacg gggaaggtca cttgctcttc catttgattg ggcgaaagga
       61 tttttagttt tacccaatac tcattgggcg cattgatgcg gtaaaactcg ttgatgactt
      121 gtgtgccaga ctcttcaaag atcaatgtgc ctacatctga ggtgacgcct gtgctggctt
      181 cccattgcca attgaggagg gttggtccgt tggcggtaat gttggcttca aagaagacgg
      241 tctgcggaaa gtcattgcag ttgacgacga tacgattcgg ctccacgcgc aaatccactt
      301 tggtgacgct gacgaagggg gcatctacga taggtagttg ctccgcggtt ccttgcaagg
      361 tggtgacttc ggctgcaacc cagcaatatc cgcctgggtt gatgggatct tcgacctgcc
      421 accaggtaga tgccgcatcg cgcccgacgg cgtttaagag tctcccaccg cggacttcat
      481 tcatagtttg gtagaccgtg ccaggaccaa aacggcagtt ggcaacttct gcgttcacct
      541 gtacaggaat ctctacaagg gtgggagtgg gtggaacagg tgtgggggta ggaacagatt
      601 gagtggcagt agccggaatc ggcgtggggg gaggcggcgt acttttcgtt gtgcatccca
      661 ccaaggtgat aatgagtcct gcaagcagga cgaggcggtt catttttcct ccttttgtca
      721 ttcgtttcaa ctctccatcg gcgttggaga ggcaaggcaa ttcggggtcg aatcacctgc
      781 atgttctata gaagtgtaac agggaagctg aagttcagat gcgctcatag aatggaattg
      841 gtactttgtg gggataagca attgcaaaat tagaaaattt gttctataat tgtaccattg
      901 tgtaactcgg ttgttttatt caaaaggaga tgagagtatg agtagtcttg ataaagccgt
      961 tcaaacacaa ctggaaaaca tccaaaagaa gaccggtaaa tcgctggcgg agttggcagc
     1021 cattgctcaa aagagtgggc tgaccaagca tggcgaactg cgcgatatgt tcaaggagaa
     1081 actggggctt gggcatggcg acgcaaatgc tctggtgcat gcggttttca agtctgatgg
     1141 tacacgtgct gccgaaggga aaagctctga tgctgtttta gatgaaattt acgtcggtgc
     1201 caaagcctcg cagcgcccca tccatgaggc gttgatgaaa cagatcaaca agttcggaga
     1261 gtttgaggtt gtgccgaaga agggatatgt aagtttgcgg cgcaagaagc aatttgcgat
     1321 gatcggtcct aaaaccaata ctcgttttga agtgggtatt aatgccaaag agatgaagaa
     1381 aaatccgcgt ttggtagaac aacccaaagg cagtatgtgc aattacatcg ttgccctgac
     1441 ggatgccagc gaagtggatg ctgaactgat tggctggatc aaacttgcat atgaaggagc
     1501 tgattaatgt cggaagattt tagaaaaaat cttttgaact ttatgaccga gacctttgaa
     1561 agccccatgg gaatttacct tgataagaac acggctctgt ttcagacgct cgaaacggtc
     1621 tctgcagcgg aggcttcgat tcccgttggc gggaagtgcg ccacgctcgc ggcacaggtt
     1681 gcgcatgtga cctattatct tgatgtgctg gagcgttatg tagttcacaa cgatacatcg
     1741 cctgccgatt ggggcgaggt ttggcgcacg gtgagcggcg tcacgacgca agaatgggaa
     1801 acgagtaaag ccaaattgcg aagtgcctat caacgcatgg tggagttatt caagacaaac
     1861 ccagtttgga acgaaaccac gatgggcgga gccatggcaa ttgttgttca cagtgcctat
     1921 cacctaggag agatacgtca ggcgctttgc acgttgaaaa aataaaaaag aggaaacata
     1981 cacatgtcga ccaatcatgc ccattaccta attgaagaat acgcctttca taactggttg
     2041 gttcatcaca tccttgaagg gatcga
//
LOCUS       DAOQFT010000471         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_36977_length_2058_cov_6.245632, whole genome shotgun sequence.
ACCESSION   DAOQFT010000471 DAOQFT010000000
VERSION     DAOQFT010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36977_length_2058_cov_6.245632"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLR93_10930"
     CDS             <1..320
                     /locus_tag="PLR93_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH27527.1"
                     /translation="RREAMALRRIYPESNFNLYDFLRLTITNILSDLWHAARERVLLK
                     SFSSIFWFRFAQFRGTYMGHRETSLVTPQLRETFYYARERKKKDERKRDVEPIQYNKG
                     NDK"
     gene            358..942
                     /locus_tag="PLR93_10935"
     CDS             358..942
                     /locus_tag="PLR93_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNH27528.1"
                     /translation="MATPPPNSVHPKSRAEWRKWLEENQARKEGIWLISYKKDTGKPR
                     FDYDESVEEALCFGWVDSKPAKLDEERSMLWFAPRKPKTNWSKLNKDRVEKMIKAGLM
                     APAGLAKVEAAKKDGSWNALDGVEALEIPPDLAKALSKNKTAKEYFEAFPRSVKRGIL
                     EWILNAKRPETRAKRIEETVTLAEKNVRANQWRQ"
     gene            988..1815
                     /locus_tag="PLR93_10940"
     CDS             988..1815
                     /locus_tag="PLR93_10940"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH27529.1"
                     /translation="MPTLSLKPASEYTIPFLADLMTRGFEGYIVPINITDNVLHTMIR
                     RDGIDLTSSRVLVRDEEPIGVAFIARRGWTSRLAAFGITSNARNGGVGTWAMEQLLQE
                     ARERGEKEMLLEVIEQNTAGVKLYERVGFTKIRRLVGYKLENPQVESDVELEEIDIRE
                     LARLVAYHGLKDLPWQLSGATVMQHTPPSRAFHVNDAYCLITNPEATDIAISSVLVKT
                     RSRGAGLSAVLLRALFAKFPNKVWHVSPIYPEEMGVIFEHVGMQRETLSQWQMSRVL"
     gene            1825..>2058
                     /locus_tag="PLR93_10945"
     CDS             1825..>2058
                     /locus_tag="PLR93_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNH27530.1"
                     /translation="MKLAALVPMRHHSQRVPGKNYRPLAGKPLFQHILETLQAVPEID
                     TVIVDTDSEPVMDGVRRLFPNVKLIQRPEHLRAD"
BASE COUNT          570 a          512 c          497 g          479 t
ORIGIN      
        1 atcgccgtga agccatggcg ttgcgacgta tctatcctga gtcgaatttc aatttatatg
       61 atttcctgcg cttgaccatc acgaatattc tgagtgatct ttggcacgcc gcccgcgaaa
      121 gagttttgtt gaaaagtttt tcatccatct tctggtttcg ctttgctcaa tttcgaggaa
      181 cttatatggg acaccgcgag acaagtctgg tcacgccgca gttgagagag actttttatt
      241 atgctcggga acgaaagaag aaagatgaaa gaaaacggga cgtagaaccg atacaataca
      301 acaaagggaa tgataaataa gtaatatgcc gttgaaaaag actgtctatt atgtactatg
      361 gctactcctc cccccaactc cgttcaccct aaatcacgag cagaatggcg caagtggctg
      421 gaagagaacc aggcgcgcaa agaaggtatt tggctaatca gctacaagaa agataccggc
      481 aagccgcgct tcgactatga cgaatccgtg gaagaagccc tatgctttgg ctgggtagat
      541 agcaaacctg ccaaacttga cgaagaacgc tccatgttgt ggtttgcccc gcgcaagccc
      601 aaaacgaatt ggtcgaagct caataaagac cgcgtggaaa aaatgatcaa agccgggctc
      661 atggctcctg ccggtctggc taaagtggaa gccgccaaaa aagacggctc ctggaacgcc
      721 ctcgatggtg tagaagcact tgaaattcca cccgaccttg ccaaagccct ctctaaaaat
      781 aaaacagcca aagaatactt tgaagcattc ccgcgctcgg tcaaacgcgg catcctggaa
      841 tggatactca acgccaaacg accggagaca cgggcaaaac gaattgaaga gacagtgacg
      901 ctggcagaga aaaatgtccg cgcgaatcaa tggcggcaat aagtctaaag aggttttgat
      961 ataaggatat tcgatataat ttccactatg cctacccttt ctctaaaacc tgcttctgaa
     1021 tacaccatcc ccttcctcgc agacttgatg actcgcggat ttgaagggta catcgtgcct
     1081 atcaacatta cagataatgt attgcacacc atgattcgcc gagatggcat tgacctgaca
     1141 tctagccgtg tgcttgttcg cgatgaggag ccgataggcg ttgcatttat cgcccgccgc
     1201 ggctggactt ctcgcctcgc cgcatttggc attacgtcca atgctcgtaa tggtggtgtg
     1261 ggaacatggg caatggaaca acttctgcaa gaagctaggg aacgcggcga aaaggaaatg
     1321 cttctagagg taatcgaaca gaataccgca ggcgtgaaac tgtatgaaag agtaggcttt
     1381 actaaaatcc gcaggctggt gggctataaa ctcgaaaacc cgcaagttga atccgatgta
     1441 gaactcgaag aaattgacat tcgtgaattg gcgcgtttgg ttgcttatca tggactcaaa
     1501 gaccttcctt ggcaactttc gggtgctact gtcatgcaac acacaccgcc ttcacgcgca
     1561 tttcacgtca acgatgccta ttgtcttatc accaaccccg aagcgaccga catcgccatt
     1621 tcgtcggtgt tggtcaaaac ccgctcacgt ggagcaggct tgagcgcggt tctgttgcgc
     1681 gcactttttg cgaagttccc aaataaagtt tggcacgtct cgccgatcta tcctgaagaa
     1741 atgggcgtta tcttcgagca tgtgggcatg caacgcgaga cgctttcaca atggcaaatg
     1801 tcacgcgtgt tgtaaaataa gggcatgaaa ttagccgccc ttgttcccat gcgtcatcat
     1861 agccagcgtg tgcctggcaa aaattatcgt ccgctcgcag gcaaacccct ctttcaacat
     1921 attttagaaa ccctgcaagc cgtgccagag attgacacgg ttattgtgga taccgactca
     1981 gagccagtga tggatggagt gcgccgcctc tttcctaacg taaaactgat tcaacgcccc
     2041 gagcatctcc gtgctgac
//
LOCUS       DAOQFT010000472         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37006_length_2057_cov_5.473027, whole genome shotgun sequence.
ACCESSION   DAOQFT010000472 DAOQFT010000000
VERSION     DAOQFT010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37006_length_2057_cov_5.473027"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /gene="arsB"
                     /locus_tag="PLR93_10950"
     CDS             complement(<1..715)
                     /gene="arsB"
                     /locus_tag="PLR93_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNH27531.1"
                     /translation="MTNEKRKELSFLDRYLTLWIFLAMAVGVGIGFLFPKAVADFNMA
                     VSVGTTNIPIAIGLILMMYPPLAKVHYDELGDVFRNWKILSLSLIQNWVIGPILMFVL
                     AIIFLRGYPEYMTGLILIGLARCIAMVIVWNELAKGDSEYAAGLVAFNSIFQVLFYSV
                     YAYVFITVLPTWFGMTGNIVDITIGEIAKSVFIYLGIPFLAGFFTRFFLVRAKGKDWY
                     HNVFVPKISPMTLIALLFTI"
     gene            complement(728..1123)
                     /locus_tag="PLR93_10955"
     CDS             complement(728..1123)
                     /locus_tag="PLR93_10955"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNH27532.1"
                     /translation="MKKVLFLCTGNSCRSQMAEAIVNARMGAEWYAVSAGTKPAGYIH
                     PKALTVLSEIGIQHEGRSKRADEFQDESFDLVVTVCDSAAEECPVWLGKGKKVHHSFP
                     DPALTDDMNDFRAVRDDIEKVILELLKTN"
     gene            complement(1202..1453)
                     /locus_tag="PLR93_10960"
     CDS             complement(1202..1453)
                     /locus_tag="PLR93_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH27533.1"
                     /translation="MLTIKVLGSGCANCKKVEAIAHQVVDQMGIQAEIIKVIEYPDIM
                     AYDIMSTPGLVINEKVVSYGRIPSPAEVTTWITNALQTA"
     gene            complement(1465..>2057)
                     /locus_tag="PLR93_10965"
     CDS             complement(1465..>2057)
                     /locus_tag="PLR93_10965"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNH27534.1"
                     /translation="IILLFGLFGWKVAGLYVASGLVVAIVAGFIIGRLKMEKYVADFV
                     WKVHRKVNVTEEKLTWSDRIERAWQSVKEIVGKVWLYVVVGIAVGAGIHGYVPEDALA
                     SILGAQAWWSVPLAVLIGIPLYSNAAGIVPIVGTLIEKGASVGTALAFMMSVIGLSLP
                     EAVILKRVLKMPLLVTFFVVIAIAIIFTGYLFNLIL"
BASE COUNT          570 a          561 c          442 g          484 t
ORIGIN      
        1 ctatagtgaa gagtaaagca atcaaggtca ttgggctgat cttcggcaca aagacgttgt
       61 gataccaatc cttacccttg gcacgtacaa ggaagaaacg agtgaagaaa cctgctagga
      121 acgggatgcc gagataaatg aagacactct tcgcgatctc gccaatggta atgtccacga
      181 tattgccggt catgccgaac caagttggca agacggtaat aaagacatag gcatatacac
      241 tgtagaaaag cacttggaag atgctattga aagcgaccaa tcccgccgcg tattcgctat
      301 cgcctttggc aagttcgttc cacacgatga ccatcgcgat acaacgcgca agtccgatca
      361 aaatcaagcc cgtcatatat tccggatagc cgcgcaagaa gatgatcgca agaacgaaca
      421 tcaagatcgg accgatcacc cagttttgaa tgagcgagag gctgagaatc ttccaattgc
      481 ggaagacatc gcccaactca tcgtaatgga ccttcgccag cggcggatac atcatcagga
      541 tcaatccgat ggcaatcgga atattcgtcg ttcctaccga aacagccatg ttgaaatctg
      601 ccaccgcttt ggggaagaga aagccaatcc ccacccccac agccatggca aggaaaatcc
      661 acagggttag atatcggtcg agaaatgaaa gttccttgcg cttttcgttt gtcattttgc
      721 ctccgtatta attcgttttt agcaactcca gaatcacttt ttcaatatca tcgcgcacag
      781 cgcgaaagtc attcatatca tcggttaatg ccgggtcggg gaaactatga tgaaccttct
      841 tccccttccc caaccagacg ggacattcct ctgccgctga atcacaaaca gtgacgacga
      901 gatcgaagct ttcgtcttga aattcgtctg cgcgcttcga cctaccctcg tgctggatac
      961 caatttcaga aagcacagtc aacgccttgg ggtggatgta acccgcaggc ttcgtccccg
     1021 cgctgacggc ataccactct gcgcccatcc gcgcattgac gatggcttct gccatttgcg
     1081 aacggcagga atttccggtg cagaggaata aaactttttt catggtcttc tctccttatt
     1141 aatggtgttc aatcttttac cataaggaga actcttgaat tgttatggtt tggttaatgg
     1201 ctcatgctgt ttgcaacgcg ttggtgatcc atgtggtcac ctccgcaggc gaagggatgc
     1261 gtccatacga aacgaccttt tcattgatga ccagccccgg tgtggacatg atgtcatacg
     1321 ccatgatatc gggatattca atcactttga taatctccgc ttgaatcccc atctggtcaa
     1381 cgacttgatg agcaatggct tccacttttt tgcaattggc acaccctgag cctagaactt
     1441 tgatggttaa catttcattt tcctttacaa gatcaaattg aataaatagc cagtgaagat
     1501 gatcgcaatg gcgatcacga caaagaacgt gaccaaaagc ggcatcttca aaacacgttt
     1561 gagaatcacc gcttcgggca aacttaagcc gatgacagac atcatgaatg ccagagctgt
     1621 tccaactgaa gcgcctttct cgatcaacgt tccaacgatg ggaacgatcc ctgccgcatt
     1681 ggaatacaac ggaataccga tcaacaccgc caacggaacc gaccaccacg cctgcgcccc
     1741 caaaatagat gccaatgcat cttctggcac gtagccatga atgcccgccc ccactgcaat
     1801 acccaccacc acatacaacc agaccttgcc cacgatctct ttcactgatt gccatgcgcg
     1861 ttcgatacga tcggaccagg tcagtttttc ttcggtcaca ttcacttttc ggtgaacctt
     1921 ccagacaaaa tctgccacat acttttccat tttgagtcgc ccaataatga agcctgccac
     1981 aatggcaacg accaaccccg aagcgacata caaccctgct accttccagc cgaacaagcc
     2041 aaacagcagg atgatcg
//
LOCUS       DAOQFT010000473         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37107_length_2054_cov_6.871936, whole genome shotgun sequence.
ACCESSION   DAOQFT010000473 DAOQFT010000000
VERSION     DAOQFT010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37107_length_2054_cov_6.871936"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..976)
                     /locus_tag="PLR93_10970"
     CDS             complement(140..976)
                     /locus_tag="PLR93_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNH27535.1"
                     /translation="MSNTKTILPTEHCETCPAFTQLEQMGIKVGDFDRVVALAGNPNT
                     GKSTLFNALTGLKQHTGNWPGKTVTRAEGGFEFNKLRYKLVDLPGTYSLLSASQDEEV
                     ARDFILFGQPNCTIVVTDATALERNLNLVMQVMEITENVVVAVNLMDEARRKGLEVDA
                     RSLSRDLGVPAIPVTARTGEGIHTLLGAVAEVIEGKVETKPLRVNGTPEFQNAVNELV
                     PMIKQLAPGIPNARWLAIRLLDGDARVQKAITSGELEEIVSRQYQQDAQFSKKMSVEG
                     RQ"
     gene            complement(987..1982)
                     /locus_tag="PLR93_10975"
     CDS             complement(987..1982)
                     /locus_tag="PLR93_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HNH27536.1"
                     /translation="MYYTIAAITLLVLIFMPRIGLLALYKNYRTSQIREQVEDALKHL
                     LDRERQGRHASPESLGGTLDLQRARVTHLIQDMEAQGLVESNGNELHLTAKGERWALH
                     VVRAHRLWERYLADEARMPLNKIHDEAQKREHSLTEAQLNELDAALGHPTRDPHGDPI
                     PTREGKMNQTEGKPITAWQADRPARIVHLEDEPALAYQQILAAGLRLGQDIRVIEKTP
                     NRIVLSDGENEYRLAPAVAYNIHVAAIPESEMLKREAVPLFELEHDQRGEIVTLDDAV
                     QGFTRRRFLDLGLTPGTVIYPELKNFFGDPRAYRVRGTLIALRKDQAAQIWVKPI"
BASE COUNT          459 a          502 c          519 g          574 t
ORIGIN      
        1 ggttttgacg gcgcgatgag caattttttc tgcttcacta taaagggatt tgacgatctc
       61 atcgcgaaag ccggttgata aactattgcg taaggcttcg gcttttgcca agacatcgtt
      121 gggttgtaaa ttttccatat tattgccgtc cttctacaga catcttcttg ctaaattgcg
      181 catcttgctg atattggcgc gatacgattt cttcaagttc gccgctggtg atggcttttt
      241 gtacacgtgc gtcaccatcc aacaagcgga tcgccaacca gcgtgcgttg ggaataccgg
      301 gagccagttg tttgatcatg ggcaccagtt cattaacggc attctgaaat tcaggagtgc
      361 cattcacgcg caatggcttg gtttccactt tgccttcgat cacttcagca acagcaccta
      421 ataaagtatg gattccttcg ccagttcgtg cagtgactgg aatggcaggt acgcccaagt
      481 cacgcgagag agaacgagca tccacttcga gacctttgcg gcgggcttcg tccatgaggt
      541 tgacagcaac gactacattc tcagtgattt ccattacttg catcacaagg tttaggttgc
      601 gttcaagcgc tgtggcatcg gtcacaacaa tggtgcagtt tggttgacca aacagaataa
      661 aatcgcgtgc cacttcttca tcttgtgaag cagacaataa agaatacgtg ccgggcaggt
      721 ccacaagttt gtagcgcaat ttgttgaatt caaagccgcc ctcggcacgg gtgacggtct
      781 tgccaggcca gttgccggtg tgctgcttga gcccggtcaa cgcattgaat aaagtagatt
      841 tgccagtgtt tgggttgcca gctaatgcaa ccacacggtc gaagtcgccg actttgattc
      901 ccatttgttc gagttgcgta aatgcggggc aggtctcgca atgttcagtg gggaggatgg
      961 tttttgtatt agacatgatt cgttccttat atgggtttta cccaaatttg tgctgcctga
     1021 tctttacgta atgcgatcaa tgtgccacga acgcgataag cgcgtggatc gccgaagaaa
     1081 tttttcagct cagggtaaat cacggttcca ggagtcagcc cgaggtccag aaagcggcgg
     1141 cgcgtgaagc cttgcacggc atcatccaga gtcacaattt cgccacgctg atcgtgctcc
     1201 aattcaaata acggaacggc ttcacgcttt agcatctcgc tctcaggaat tgccgccaca
     1261 tgaatgttat atgccacagc cggcgcaagc cgatattcat tttcgccgtc actcaacaca
     1321 atgcggttcg gtgttttttc aatcacgcgg atgtcttgcc ccaagcggag ccctgccgcc
     1381 aggatttgtt gatacgccag agcgggttca tcctctaagt ggacgattct tgccggacgg
     1441 tctgcctgcc acgccgtaat gggctttcct tctgtctgat tcattttccc ctcacgcgtt
     1501 ggaatcgggt cgccatgtgg gtcgcgagtg gggtgaccca atgcggcatc gagttcattt
     1561 agttgtgcct cagtgagaga gtgttcacgt ttctgcgctt cgtcatggat cttgttcagt
     1621 ggcatgcgtg cttcatctgc caagtaacgc tcccataaac gatgtgcacg tacaacatgc
     1681 aatgcccagc gttcgccttt ggcagtgaga tgtaattcat tcccattcga ctcaaccaaa
     1741 ccttgagctt ccatatcttg aatgagatgg gtgactctgg cgcgttgaag gtctagggtg
     1801 cctcccaaag attctggaga ggcgtgcctt ccttgtcgtt cacggtctag aaggtgtttc
     1861 agagcatctt ccacctgctc gcggatttga ctggtacggt aatttttata aagcgccaat
     1921 aatcctatgc gcggcatgaa gatgaggacc aataatgtaa tcgcagcaat ggtgtaatac
     1981 atagtttttt tccttgctta tttgaatttc gggaagaatt ttcccgtccg ttgcatatag
     2041 tcgcgatatt catc
//
LOCUS       DAOQFT010000474         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37191_length_2051_cov_5.594188, whole genome shotgun sequence.
ACCESSION   DAOQFT010000474 DAOQFT010000000
VERSION     DAOQFT010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37191_length_2051_cov_5.594188"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLR93_10980"
     CDS             complement(<1..280)
                     /locus_tag="PLR93_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27537.1"
                     /translation="MNPQSLLKINPNLIDSVNSVLLDPNSRVMKNFFDVVAKYGTPEE
                     INRKHRESRKMENLFKQVEAKAPEYLKDLNWLIQQRDSGRFISVAEYRQ"
     gene            complement(365..1222)
                     /gene="panB"
                     /locus_tag="PLR93_10985"
     CDS             complement(365..1222)
                     /gene="panB"
                     /locus_tag="PLR93_10985"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNH27538.1"
                     /translation="MTEPRKKITLPALYQKAAESKPITWLTCYDYPTAYMQDQAGVDM
                     ILVGDSLGMTMLGYDSTLPVTMDDMIRHAAAVRRGAPNVWLVGDMPYMTYQPSVESAI
                     RNAGRFMAEAGCDCIKLEGGAAMADRIKGIVDAGIPAIGHLGLTPQSVSALGGFKLQG
                     KSAALAKKIVDDAKALEEAGAFMILLELVPDRLCQLITERAKNTIIMSLGSGPHAHGQ
                     LLIYHDAFALYPKFKPRMAKVFADAGAVIREGLNGYVKEVTEKTFPAKENWFGMPDEE
                     YDELLKMLN"
     gene            complement(1272..1754)
                     /locus_tag="PLR93_10990"
     CDS             complement(1272..1754)
                     /locus_tag="PLR93_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNH27539.1"
                     /translation="MIKNSQENMLDEIDLALLRSLQTEGRLSNVELARKINLSPPATL
                     ARMRRLEKDGYIRQYTAVVDREKAGYDLLCFIHIAMQMHQYEQVDKFRKLMQKMPEVL
                     ECHHITGEYDYLLKVVLRNRKDLERFVVDRLTPIPGVSRIHTSLVFTEVKSTTALPLE
                     "
BASE COUNT          486 a          499 c          505 g          561 t
ORIGIN      
        1 tttgcctgta ttccgcgact gaaatgaatc tgccgctgtc gcgttgttga atgagccagt
       61 tgaggtcttt gagatattca ggcgctttgg cttccacctg tttgaagaga ttctccatct
      121 tgcgcgattc acgatgtttg cggttgatct cttcgggtgt tccatacttg gcaacaacat
      181 cgaagaaatt cttcatcacg cgcgagttcg ggtcaagcaa aacggagttg acagagtcaa
      241 tgagattggg attgattttc aaaagagatt gagggttcat tttgatttcc ttatgttaga
      301 aggttagaaa attgaaaggc tataacttgc gaacctgtaa accttccaac ttgcaacctg
      361 caactcaatt caacattttc aacaattcgt cgtactcttc atcgggcatt ccaaaccaat
      421 tctctttagc ggggaaggtc ttctcggtca cttctttgac gtagccgttg agtccctcgc
      481 gaatgactgc acctgcatcc gcaaagactt ttgccatgcg tggcttgaat ttgggatata
      541 gggcaaatgc gtcgtgatag atcaggagtt gtccatgcgc gtgaggacca gagccgagcg
      601 acatgatgat ggtgttcttc gcgcgttcgg tgatgagttg gcataacctg tcggggacaa
      661 gttcaagcag gatcatgaac gcgcccgctt cttcgagtgc cttggcatcg tccacgatct
      721 tctttgccag cgccgcagac tttccctgca acttgaaccc gcccagagcg gagacagatt
      781 gaggcgtgag tcccaaatga ccaatggcag gaatgcccgc atccacaatc cctttgatac
      841 gatccgccat cgccgcaccg ccttcgagtt tgatgcagtc acaacccgct tccgccataa
      901 atctccctgc gttgcggatg gcggactcaa cgcttggttg gtaggtcatg tacggcatgt
      961 cgcctaccag ccaaacattc ggcgcgccac gacgcactgc tgccgcgtga cggatcatgt
     1021 catccattgt gacgggcagg gtcgagtcgt agccgagcat ggtcatgcca aggctgtcgc
     1081 cgacgaggat catatccacg cctgcctgat cttgcatata tgcggttgga tagtcgtaac
     1141 aggtgagcca agtgatgggc ttgctctccg ccgccttttg atagagagcg ggtagagtta
     1201 ttttcttgcg tggttcagtc attcgttcct ccgtgtgcaa attttttcgc atcacacacg
     1261 gaggcttttg cttactccaa tggcaatgcg gttgtagact tgacttcggt aaacaccaaa
     1321 cttgtgtgaa tgcgcgagac gccaggaatg ggcgtgaggc gatcaacaac gaagcgttcg
     1381 aggtctttgc gattgcgcag aacaactttc agcagatagt cgtactcgcc agtgatgtga
     1441 tgacactcca gcacttcggg catcttctgc atcaacttgc ggaacttatc cacttgctcg
     1501 tattggtgca tctgcatcgc aatatggatg aagcacagca ggtcgtagcc agccttttca
     1561 cgatccacta cagccgtgta ctggcggatg tagccgtctt tttccagccg cctcatacgg
     1621 gcaagtgttg ctggtggaga gaggttgatc ttgcgcgcta attccacatt gcttagacgc
     1681 ccttcagttt gaaggctgcg aagcagagca aggtctatct catccagcat gttttcctgt
     1741 gaatttttta tcatgaacgg cctcttgatt caatattgtt ttgtaattta ttgaaaactc
     1801 aaaactaatt ataaaataaa tgtgtttctg aaaaataaaa ttcgttttca gcgaagcagt
     1861 aaagtcttgc ttgaaagaat cgaaggtgac aaataagaaa aaaatgcccg caaattttcc
     1921 ttgcaggcat tttgagacac agaattccac tttctacttt ctaatcgtgg tggaaagtag
     1981 tttaacgtcc tttcaacggt ttgaaccctt cgcccagggc ctcatgtgcg accccgatga
     2041 ccataaatgc t
//
LOCUS       DAOQFT010000475         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37416_length_2044_cov_3.926596, whole genome shotgun sequence.
ACCESSION   DAOQFT010000475 DAOQFT010000000
VERSION     DAOQFT010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37416_length_2044_cov_3.926596"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1259)
                     /locus_tag="PLR93_10995"
     CDS             complement(144..1259)
                     /locus_tag="PLR93_10995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH27540.1"
                     /translation="MNVAIVHEWLTTFGGTERLLVECLQLFPQADVFALTHTPQNFQH
                     TALKDIKVHTTFLQNIPGIDRHYRKLLPLMPYAIESLDVSGYDLVISLSHAVAHGVKT
                     HDRQTHVSYISTPMRYAWHMRDDYLKLHGLTNPLIQFAATSTLGLLRRWDTFASARSD
                     SLIANSYWTASHIQQAWQRKADVIYPPVDVQRFVPAQQRDDYYLLVSRLVPYKMGIEI
                     TKAFNSLGLPLVIIGDGPELQHVRSIAKENITIMGYQPDAIVTEFMNKAKAFVYMATE
                     DFGIAMVEAQAAGCPVIAYCKGGASEIVRDGETGLLFKEQTSEALADAVLKFESMNLN
                     SKAASDNAARFSAERFRREFLDHIQKIASSGKAPSSQ"
     gene            1289..>2044
                     /locus_tag="PLR93_11000"
     CDS             1289..>2044
                     /locus_tag="PLR93_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27541.1"
                     /translation="MFDKNKLAENLRICARISFALMLILIPFRLRTVAIAHPRLGIFG
                     DYTDFLFYAMDVGLLALVIFWILSLMLKPRRLFLGPIAVGFPLAGLLASAMISLITSI
                     DPLLTFYLLTRYGLLLVLYLFIINELFSAVWFAVPAAIGVLLQSVIAVAQSLTQHDVK
                     LQWLGEYSLDPTAPFMSILEVNGTRFLRAYGLSDHPNILGGFLAFGMVILLSVVVARV
                     GKSWVKMMAAFVFVCGSAGLFTTYSRSAWAALVA"
BASE COUNT          482 a          447 c          521 g          594 t
ORIGIN      
        1 attgttagtt cagcaaatga agtgcaggaa caacttcatc ggttacaaga agctggctta
       61 cagcgcatca tgctccaatg gctggattta gatgatcttg ccgggctgga agctctggcg
      121 aaagcagtgt tataaggagt atgtcattgc gaggagggtg cttttcccga cgaagcaatc
      181 ttttgaatat gatctaaaaa ttcccgcctg aatctttcag cagaaaagcg ggccgcattg
      241 tctgatgcgg ctttgctgtt taagttcatt gattcaaatt tcaagactgc atccgctaag
      301 gcttcggatg tttgttcttt gaacaaaagc cctgtctctc catctcgcac gatctccgat
      361 gcgccgccct tgcaatatgc aatgacagga catcccgccg cctgcgcttc gaccatggca
      421 atcccaaaat cctcggttgc catgtacaca aaggctttgg ctttgttcat gaattcagtg
      481 acgattgcat cgggttgata ccccatgatc gtgatatttt ctttggcaat actgcgaacg
      541 tgttgaagtt cgggtccatc accgatgatg acaagcggta aaccgagaga attaaatgcc
      601 ttggtgatct caatccccat tttgtacgga accaggcgtg aaacgagaag ataatagtca
      661 tcgcgctgct gagcaggtac aaaccgttgt acatccacag gcggataaat aacatctgct
      721 ttgcgctgcc aggcttgctg aatatgggac gccgtccaat atgagttggc gatcagcgaa
      781 tcggatcggg cggatgcgaa ggtgtcccaa cggcgcagca agcccagggt tgaggttgcg
      841 gcgaactgta taagaggatt ggtcagaccg tgcaatttta aatagtcatc acgcatatgc
      901 caggcatagc gcatgggcgt cgagatatag gaaacgtgag tctgccgatc atgggtctta
      961 actccatgag caacggcatg tgaaagggaa atgacaaggt catatccact gacgtccaaa
     1021 gactcaatgg catagggcat caatggcagg agtttacgat agtgccggtc gatccctgga
     1081 atgttttgca aaaaggtcgt atgaactttg atatctttca acgcagtgtg ttgaaagttt
     1141 tgcggggtgt gagtgagtgc aaagacatca gcttgtggaa aaagttgcaa acattcaaca
     1201 agcaggcgtt ccgtgccgcc aaacgtggtt aaccattcat gaacaatagc aacattcata
     1261 gtcgggggat tatattagaa tacgccggat gtttgataaa aataaactgg ctgaaaactt
     1321 acgcatttgt gctcgcattt cctttgcgtt gatgttgatt ttgattccct ttcgcttgcg
     1381 aacggttgct atagcgcatc cacgtttggg aatttttggc gattacacag actttctttt
     1441 ttacgccatg gatgttggtc tgttggcgct tgttatcttt tggatactat ccttaatgtt
     1501 gaagccgaga cgcctctttt tgggacccat agcagttggc ttcccattgg ctggactcct
     1561 ggcctcggct atgatctcgt taataaccag catcgatcca ttattgacct tttaccttct
     1621 gacccgttat ggcttgttgc ttgttctata tctttttatc atcaatgaac ttttttctgc
     1681 ggtctggttc gctgtaccgg ctgcgattgg cgttttattg caatcagtta ttgccgttgc
     1741 gcaatctttg actcaacacg acgtgaaatt gcagtggctg ggtgaatatt cacttgaccc
     1801 aactgcgccg tttatgagca ttcttgaagt aaacgggact cgttttttgc gggcttatgg
     1861 tttgagcgac catcccaata tccttggcgg ttttcttgcc tttggcatgg tgatattgtt
     1921 gtctgtcgtt gtggcgcggg ttggaaaatc atgggtgaaa atgatggcgg ccttcgtctt
     1981 tgtgtgtgga agtgccgggc tattcaccac gtattcccgt tccgcctggg cagcattggt
     2041 ggcg
//
LOCUS       DAOQFT010000476         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37422_length_2044_cov_3.392660, whole genome shotgun sequence.
ACCESSION   DAOQFT010000476 DAOQFT010000000
VERSION     DAOQFT010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37422_length_2044_cov_3.392660"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLR93_11005"
     CDS             <1..868
                     /locus_tag="PLR93_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27542.1"
                     /translation="LSLRLTGWETPRWFGILFFFLCVTNFYTFQAILDASPVILLALF
                     FVGIVFTLSNEQDEAAGALIAFSLYYWEVGLPFLLFVVWYAYKTRRVRVFAGFGMFSF
                     IFLAASFIIFPNWLIAYLRAGMNNLRADFGYTIFSSLAVLLPAFGNWLAWGVVILLIF
                     ALGYEWNAAIQFNDPRRFYWTACLSLAAAPLLGFRSQWENLAVLFIPLALIFAIIQDR
                     WRIGRLLNFLFLFILLLVPWALYLYAIPSYGEIVKELIFLLLPLFTLVGLYWIRWWAL
                     HPPRIWTDTFTR"
     gene            923..>2044
                     /locus_tag="PLR93_11010"
     CDS             923..>2044
                     /locus_tag="PLR93_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27543.1"
                     /translation="MSRRTYFILFLCGLILPTVIAFFQPKPGYLDSEYYFAGGVQLAT
                     GHGFNEPYLWNYLDGTRSLPHPSHTYWMPLSSIVAAIPMLLTGLTTYAVARVPFILIG
                     AAIVPLTAALSFAFSQKQDVAILSAMFSIFTVYNAPFIGVTDNFGLFMLFGVLYFLTA
                     TKLIEDPARSKYWPVLGLLAGLLSLSRSDGLLWLGMTFIFTLFRAPKTTFSKLLGYVV
                     RNSIFALVGFLLIMLPWYVRNYNLFGTIMAPGGSRALWLENYDQTFIYPPEALSKESF
                     LATGWTEILSDRLWALGNNLQSGFAAHGGIILFPFIVAGILYHRRDERVKLAWIAWLI
                     LLLVMTFLFPFAGARGAFFHAGAALQPMWWVVAPLGLDGI"
BASE COUNT          374 a          535 c          448 g          687 t
ORIGIN      
        1 actgagtctg cgcctgaccg gctgggaaac cccgcgctgg ttcggcattc ttttcttttt
       61 tctttgtgtt acaaattttt acacctttca agctattctc gatgccagcc cggtcatcct
      121 gcttgccttg ttttttgtag gtattgtttt taccttatcg aacgaacagg acgaagcggc
      181 gggagcgtta atcgcattct ctttgtatta ttgggaagtg ggcttgccgt ttcttttgtt
      241 tgttgtctgg tatgcgtata agaccaggcg tgtacgcgtc tttgccgggt ttggaatgtt
      301 ctcttttatt tttcttgctg cctcttttat tatcttccct aattggctga ttgcctactt
      361 gcgggccggt atgaataatt tgcgcgcaga ttttggatat acaatttttt catcattagc
      421 cgtccttttg ccggctttcg gaaattggtt ggcgtggggc gttgtcattc tgctcatttt
      481 tgcccttggt tatgaatgga atgcagccat tcaattcaac gatccccgcc gcttttattg
      541 gacagcctgt ctttcgcttg cggctgcgcc tctgctggga tttcgcagtc aatgggaaaa
      601 cctggccgtg cttttcattc ctttagcgct gatctttgcc attatccagg atcgctggcg
      661 aattggccgc ctcctcaact ttctttttct attcatcctt ttgcttgtgc catgggcgct
      721 gtacctgtat gccattccca gttacggtga aattgtaaag gaattgatat ttctcctgct
      781 gccgcttttc accctcgttg gtttatactg gattcgctgg tgggcacttc acccgcctcg
      841 tatttggaca gacactttca cacgataact ggaaattctg caatctccat ctcttaactg
      901 ccattcaaat atcttaaatc aaatgtcccg ccgaacctat ttcatccttt ttctctgcgg
      961 cttgattctg ccaacggtta tcgcattctt tcaacccaag cctggctatc ttgattcaga
     1021 atattacttt gccggcggtg tccagcttgc gaccgggcat ggatttaatg aaccctacct
     1081 atggaactat ctggatggga cacgttcatt gccgcatcca tcccacacat attggatgcc
     1141 cctttcatcc attgtggccg ccattcccat gctactcacc gggctgacta cgtatgccgt
     1201 cgcgcgagtt ccctttattc tgatcggcgc tgccattgtc ccgttgaccg ctgcactttc
     1261 ctttgccttc tcccaaaaac aggatgttgc aatcctttcg gcaatgttct ccatttttac
     1321 agtttataac gccccattta tcggggtaac agataacttt ggcctcttca tgttgtttgg
     1381 cgttttgtac ttcctaactg caaccaaact gatcgaagac cctgcacgtt ccaaatattg
     1441 gcctgtgctc ggcctgctgg ccgggttact gtctctctcc cgaagcgatg gccttctatg
     1501 gttgggcatg acttttatct ttaccctttt ccgtgccccc aagacaacat tttcaaaatt
     1561 actggggtat gtcgttcgta attctatttt cgctttggtt ggtttcttgc tcattatgct
     1621 gccctggtat gttagaaatt acaacctctt tggcacgatc atggctccgg gcggcagccg
     1681 cgcgctctgg ctcgaaaatt acgaccaaac ctttatctat ccacctgagg ctctctccaa
     1741 agaatccttc cttgctaccg gatggacaga gattctttca gaccgtcttt gggcactggg
     1801 gaacaatctg caaagcggat ttgcagcaca tggcggaatc atattatttc catttattgt
     1861 ggctggtatc ctgtatcatc gccgcgacga gagagtcaaa ctcgcctgga tcgcctggtt
     1921 gatccttctc cttgtgatga ctttcctttt cccattcgca ggtgcgcgcg gagcattctt
     1981 ccatgcaggt gcagctttac aacccatgtg gtgggttgtg gcccctcttg ggcttgatgg
     2041 tatt
//
LOCUS       DAOQFT010000477         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37591_length_2038_cov_4.517398, whole genome shotgun sequence.
ACCESSION   DAOQFT010000477 DAOQFT010000000
VERSION     DAOQFT010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37591_length_2038_cov_4.517398"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1789)
                     /locus_tag="PLR93_11015"
     CDS             complement(161..1789)
                     /locus_tag="PLR93_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27544.1"
                     /translation="MKRPRIQRDEIASLLFGLSDESLEFRNRWRSLGMGAIWLLLSGL
                     TMLFFVFLATDNLSRVAIIGMSFIKYVPLISVVYALAKSKAAKYLDDLFELGNEDLAS
                     DFIEEVAFGYGNEKITINEGKISEKEERSPIILIGGPGMIQVNLDSVALLEKVDGEPE
                     IIYPRGEPWKLGRFERIREIGKNDEVGKREYAIINLRDQFVRGLSVRSRTKDGIPLEA
                     QDIKVMFSILRKPQSSEQESDPYHFDERAVLSLVYNQVIITPPPNTPSGVTFPWDTTV
                     IPLVTSELENLITSRNLSEILASISQKEMDVINQNEETNVMMRIEMTGEQTAVSNTPS
                     AARPPNFESRSKITSQFFSPEFREKAGALGVAIHWIDIGTWQLPSTIILDKLKDAWNL
                     MRENAKRRNTVNRSTKQHEFKAVVELVTNVVISNYSKTPSSNYSYNVPSSSRKYSEKD
                     LDELARLMEDNPEIATSPQLMRQFSQSSSSKKNSAHSTALEILKAFRKELIVAKELIQ
                     KENRSSIDKQAEIARIDKALRDINYHIYHFVKSY"
     gene            complement(1786..>2038)
                     /locus_tag="PLR93_11020"
     CDS             complement(1786..>2038)
                     /locus_tag="PLR93_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27545.1"
                     /translation="RNEAIKRFARLASEKFDNPIAPPEDIFTTLQNLMEPIKETIVLE
                     SRANNEMESELKKLDEIWKWLLVNKVDSSSHQLPEQTNS"
BASE COUNT          438 a          447 c          565 g          588 t
ORIGIN      
        1 ccggcatcgc gaacgttctt ttccatgttt cgggcaagtt ccaggttttg aggttggggc
       61 aggccgtgtg tgatgacggt cgattcaagg gcaacgatgg gcgcgcccag gttgagcgcg
      121 cgagtgattt cggtggaaag ttgaatgtgg ggatgtttca ttagtagctc tttacaaaat
      181 gatagatgtg gtagttgatg tcgcgcaggg ctttgtcgat gcgtgcgatt tcggcttgct
      241 tatcgatgga ggagcggttt tctttttgga tgagttcctt ggctacgatg agttccttac
      301 ggaaggcttt gaggatctcc aatgcggtgg agtgtgctga gtttttctta ctggagctgg
      361 attgggagaa ctggcgcatt agttgagggc tggtggcaat ctctgggttg tcttccatga
      421 gtctggcaag ctcgtccaga tctttttcag aatatttgcg gctggagctt gggacgttgt
      481 aactataatt ggatgagggc gttttggaat aattgctgat gacgacattg gtgacgagct
      541 caacgacagc tttgaattca tgctgtttgg tggagcgatt gaccgtattg cggcgttttg
      601 cattttcgcg cattaaattc caggcatctt ttaatttatc gagaatgatc gtggaaggta
      661 gctgccaggt gccgatatcg atccaatgaa tggccacgcc gagagcgcct gccttttcgc
      721 ggaattcggg actgaaaaat tgtgaggtga tcttggaacg cgattcgaag tttggcgggc
      781 gggctgcgct tggagtattg gaaacggctg tttgttcgcc tgtcatttcg atgcgcatca
      841 tcacattggt ttcttcattc tggttgatga catccatttc cttctggctg atgctggcca
      901 ggatttcgct caaattccgt gaggtgatta gattttccaa ctcactggtg acgagcggaa
      961 tgacggtggt atcccagggg aaggttacac ctgaaggagt gtttggcggc ggtgtgatga
     1021 tgacttgatt gtagaccagg gaaagcacgg cgcgttcatc gaagtggtac gggtcgcttt
     1081 cctgctcgga ggattgtggt ttgcgcagaa tgctgaacat aaccttgatg tcctgcgcct
     1141 cgagcgggat gccatccttg gttcgcgatc tgacagataa cccacgcacg aactggtcac
     1201 gtaaattgat aatggcgtat tcacgtttgc ccacttcatc atttttgccg atctcacgaa
     1261 tgcgttcaaa gcggccaagc ttccacggct cgcctctggg ataaatgatc tccggctcac
     1321 catccacctt ttcgagcagt gctacgctat cgaggttgac ctggatcatg cctggcccgc
     1381 cgattagaat aatgggcgaa cgttcctctt tttcagagat cttgccttcg ttgatcgtga
     1441 tcttttcatt gccataaccg aacgcaacct cttcgatgaa gtctgaggcc aggtcttcgt
     1501 tgcccaactc gaatagatca tccaaatatt tggccgcctt ggatttcgcc agtgcatata
     1561 ccaccgagat gagcggtaca tactttataa aactcatgcc aatgatcgca acgcgggaaa
     1621 gattatctgt ggcaaggaaa acgaaaaaaa gcatggtcaa cccgctcagt aatagccaga
     1681 tggcgcccat ccccaaacta cgccaacggt tgcgaaactc gaggctttca tccgaaaggc
     1741 caaagagcag ggatgcgatt tcgtcacgtt ggatgcgcgg acgcttcatg aattggtctg
     1801 ctccggcaat tggtgactgg atgaatccac cttattgacc agcagccatt tccaaatttc
     1861 atcgagcttt tttaattcgc tttccatttc attgtttgcc cggctttcga gcacaatggt
     1921 ttccttgatg ggttccatta gattctgtaa tgtggtaaag atgtcttcag gcggcgcaat
     1981 ggggttgtcg aatttttctg aagccagcct tgcgaaacgt tttatggctt cattgcgt
//
LOCUS       DAOQFT010000478         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37731_length_2034_cov_5.130874, whole genome shotgun sequence.
ACCESSION   DAOQFT010000478 DAOQFT010000000
VERSION     DAOQFT010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37731_length_2034_cov_5.130874"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PLR93_11025"
     CDS             complement(<1..1066)
                     /locus_tag="PLR93_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27546.1"
                     /translation="MDNLENIARKITSVLFAQQSLASAGFIAAATLNSIVGKELSHNS
                     SWAGVPTAVYLLAGAFSAFMWGYVYDAIGRRYGLVSGLSTGVVGSSLTFYSIAIHSFP
                     LFLGGMVLMGIANSAVQLGRFAAAEVNKPEHRGQAISNVVIGGTVGSIIGPFVAGPAG
                     NLVGGWGIDELAGAYLVAAVLFVIGAIVVFTGLRPDPREIGKQVAEKYPETLIKHKHV
                     RGLLEIFRQPAALVALLSMVLGQMVMVLVMVITSLHMRGHNHNLSDISAVIASHTFGM
                     YAFSIFSGRLADKWGRGTVILIGSATLVIACIAATISPDVLPLGVALFLLGLGWNFCF
                     VGGSTLLADQLSPAERARTQG"
     gene            complement(1185..>2034)
                     /locus_tag="PLR93_11030"
     CDS             complement(1185..>2034)
                     /locus_tag="PLR93_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNH27547.1"
                     /translation="TPVLIGRKDVIENELKSLSFDYKPEIVDPDNYFKLEAYAKAFYE
                     LRQRKGLTVGDALKKVRDVNVLGSMMVKMGDADAFVSGLTYDYPEVIRPALQIHHTAK
                     GVARAAGVYIMIVEDKVFLFTDATVNIDPTADDLAEIAILAADYAKKIELDPHVAFLS
                     FSNFGSTPHPLSDKVRKAVNLVKSRRPDLRVDGEMQADTAVVPEIAEERYPFSAVKDA
                     NVLVFPSLESANIAYKLLARLGNAKAIGPILLGLGAPVHVLQTGDDVNAIVQIASVAV
                     MDAMGR"
BASE COUNT          564 a          520 c          498 g          452 t
ORIGIN      
        1 agccttgggt gcgggcgcgt tcggcgggag agagttgatc ggcgagcagg gtagaaccac
       61 cgacgaagca gaagttccaa cccaaaccaa gcaagaacaa tgccacgcca agcggaagaa
      121 catctggtga gatcgtagcc gcaatacagg caatgaccag ggttgccgaa ccaatcaaaa
      181 tcaccgtgcc acgcccccat ttatcggcaa ggcgcccgga aaaaattgaa aatgcataca
      241 taccaaaggt gtgagaggcg atcacggcag aaatatcgga gaggttatgg ttatgtccgc
      301 gcatgtgcag ggatgtgatg accatcacca acaccatcac catttgaccc aacaccatac
      361 ttagcaaggc gaccaatgcc gcaggctgac ggaaaatttc aagcaagcct cgtacgtgtt
      421 tatgcttgat cagcgtttct ggatattttt ctgccacctg tttgccaatc tcgcgcgggt
      481 ctggtctcag acctgtgaag acaacgatcg ctccgatcac gaacaaaacc gctgcgacaa
      541 gatatgcgcc agccaattcg tcgatgcccc aaccgccgac gaggtttcca gcaggtcctg
      601 ccacgaaggg accgatgatg gaaccaaccg tcccgccgat aacgacattt gaaatggctt
      661 gcccacgatg ctcaggtttg ttaacttctg cggcagcaaa gcgaccaagt tgcactgctg
      721 agtttgcaat gcccatgaga accattccgc ctaggaacaa gggaaaagaa tggatggcga
      781 tcgagtaaaa agtcaggctt gatccgacaa cacctgtgct tagcccgctg acgagtccat
      841 atcgtctgcc aatggcatcg taaacatatc cccacataaa agcagaaaac gctccagcca
      901 ataaataaac agctgtgggc acacctgccc aactggagtt atgacttagt tctttgccaa
      961 caatggagtt taaggttgcc gctgcgataa accccgcaga agcaagggat tgttgagcaa
     1021 ataatacgga tgtgatctta cgggcgatat tttcgagatt atccacgaaa tcatttcctt
     1081 tgggtgaggt ggtttggatt atacactttt gaagcggaga gaagaaaaga ccccagtaaa
     1141 gattactggg gtctcaacta tgatttgact agccaactat agaactatct gcccatcgca
     1201 tccatcacag ctacggaagc gatctgtaca atggcattaa catcgtcgcc ggtctgaagg
     1261 acatgcactg gagcgcctaa gcccaagagg atgggaccga ttgccttggc attgcccaga
     1321 cgagccagca atttgtatgc gatgtttgct gattcaagag atgggaaaac caatacattg
     1381 gcatctttca ccgcactaaa cggatagcgt tcttcagcga tctcaggcac cacagcggta
     1441 tcggcttgca tttcaccgtc cacgcgcaag tcaggtcggc gagacttgac gaggttgacc
     1501 gccttgcgaa ccttatctga aagcggatga ggcgttgaac caaagtttga gaatgaaaga
     1561 aatgccacat gcgggtcaag ctcgatcttc ttggcgtaat ctgcggcaag aatggcgatt
     1621 tcagccaaat cgtctgcggt cgggtcaatg ttcacggtgg catcggtgaa gaggaatact
     1681 ttatcttcca cgatcatgat gtacacacct gcggcacgtg ccacaccttt tgctgtatgg
     1741 tgaatttgca atgctgggcg aatcacctct ggataatcat aggtcaagcc agagacgaag
     1801 gcatccgcat cgcccatctt caccatcatt gaacccaata cattaacatc gcgtaccttc
     1861 ttaagtgcat ctccaaccgt caaccctttg cgttggcgca attcgtagaa cgccttagca
     1921 tacgcttcca gcttaaagta attatcgggg tctacaattt caggcttgta atcgaagcta
     1981 agtgacttaa gctcattctc gatcacatct tttcgaccga tcaataccgg ggta
//
LOCUS       DAOQFT010000479         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37885_length_2029_cov_6.007599, whole genome shotgun sequence.
ACCESSION   DAOQFT010000479 DAOQFT010000000
VERSION     DAOQFT010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37885_length_2029_cov_6.007599"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLR93_11035"
     CDS             complement(<1..445)
                     /locus_tag="PLR93_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HNH27548.1"
                     /translation="MTSIVLILFLLALIFFWQSNRQQKAAGLPGGRVIYTDTRGWGKV
                     EKPLFYATLGLTGKPDYLIEKNGQIIPVEVKSGRAPEAPYDSHIFQLASYCLLVEKTY
                     GRRPPYGIIHYENRDFAIDYTRELETALIELIADMREDDVRRDVAR"
     gene            complement(442..678)
                     /locus_tag="PLR93_11040"
     CDS             complement(442..678)
                     /locus_tag="PLR93_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27549.1"
                     /translation="MRPIRSSEIGSYLYCRRAWWYRKIGTPSENKAELAAGTELHRKH
                     GRQVLASALTRTLGWLLLLAAILTLVAYLAFQVL"
     gene            777..1328
                     /locus_tag="PLR93_11045"
     CDS             777..1328
                     /locus_tag="PLR93_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNH27550.1"
                     /translation="MTKHDTYSIDIAGVKRELRLFEIKPGLRIAILNILGDTELVQAC
                     ARGLADKVKNVEYDIIVTAEAKSIPIAHALSVETKKPYVVLRKTYKPYMGDALKAETL
                     SITTGQPQVLILDEKDRELIQGKKVLVVDDVISTGSTLQGMRMILDKANATVAGEAAI
                     LTEGDRSQWMNIHSLGHLPLFTD"
     gene            complement(1357..1599)
                     /locus_tag="PLR93_11050"
     CDS             complement(1357..1599)
                     /locus_tag="PLR93_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNH27551.1"
                     /translation="MSCFCYILECADGTFYTGWTTDPERRVSQHNKGVGARYTRTRRP
                     VRLVYLEEQPDKITALKRERAIKALSRKKKMELFMM"
     gene            complement(1596..>2029)
                     /locus_tag="PLR93_11055"
     CDS             complement(1596..>2029)
                     /locus_tag="PLR93_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27552.1"
                     /translation="MASSYILPRLASVALLNGTMSQPGWNEVKKSASRGYFAATSALL
                     DNSLEGLAMLAEHNRANTIHSLNVMINSLLDLRDDLENEDHASFVKRLKSAKEGRESW
                     LVEREKADWSRIPGEEIEKPSFMESLFGSKLGKLGKDKGKG"
BASE COUNT          474 a          480 c          555 g          520 t
ORIGIN      
        1 accgagccac atctctgcgg acgtcgtctt cgcgcatatc ggcaatgagt tcgatgagtg
       61 ctgtttccag ttcacgagtg tagtcgatgg caaagtcgcg gttctcgtag tggatgatgc
      121 catagggtgg acgcctgcca taggtctttt caacgagcag acaataggaa gctaactgaa
      181 aaatatgcga gtcatacggc gcttcaggcg cacgccctga cttaacctcc acggggatga
      241 tttgtccgtt cttttcgatc aaataatcgg gtttgcctgt cagcccgagg gttgcataga
      301 acagcggctt ttccactttg ccccaaccac gcgtgtcggt gtagatgacg cgtccgccgg
      361 gcaagccggc ggctttctgc tggcggttcg attgccagaa gaagatgagg gcaagcaaaa
      421 aaaggatgag gacgatggat gtcatagcac ttgaaaagcg agatacgcta ctaaagtaag
      481 aatcgctgct agaagcagga gccagcccaa ggtccgcgtg agagccgagg caaggacttg
      541 gcgtccgtgt ttgcggtgga gttcggttcc ggcggcaagc tccgctttgt tctcggaggg
      601 tgtcccgatc ttgcgatacc accaggcgcg gcgacagtag aggtaagaac caatttcaga
      661 ggagcggatc ggtcgcattt gggtagtata gcacaaattt tctaattgaa ttgaggcggg
      721 aacataatcg aatcgtgata aactttcggt gcaaaatttc cccgtggaga cgtcttatga
      781 cgaaacatga tacttactca attgatattg ctggcgtaaa acgtgaatta cgcttgtttg
      841 aaattaaacc tggtttgcgc attgcgatcc tgaatatttt gggtgacact gaattggtgc
      901 aagcctgcgc ccgcggactt gccgataaag tgaagaatgt tgagtacgac attatcgtga
      961 cggctgaagc aaagtccatt cccattgcgc atgcgctttc ggtggagacc aagaagccct
     1021 atgtggtgtt gcgcaagacc tataaaccgt atatgggcga tgctctcaaa gcggagacgc
     1081 tttcgatcac cacgggtcag ccgcaggttt tgatcttgga tgagaaggac cgtgagttga
     1141 ttcaaggcaa gaaggttttg gtagtggatg atgtcatcag caccggttcg actctgcagg
     1201 gcatgcgcat gatcctcgat aaagccaatg cgactgtggc tggtgaagcg gcgatcctca
     1261 ccgagggcga ccgttcccaa tggatgaata tccattcgct ggggcattta cctttgttta
     1321 ctgattaaat gcgtaagggc gactcgcgag tcgcccctac atcatgaaca attccatttt
     1381 cttttttctt gagagagcct tgatggctct ctctcgtttt aacgctgtga ttttatcggg
     1441 ctgttcttcg aggtagacca gtctcacggg gcggcgggtt cgggtgtagc gtgcgccaac
     1501 gcctttgttg tgctgagaca cgcgtcgttc tgggtcggtg gtccagccgg tgtaaaaggt
     1561 tccgtctgca cattcaagga tgtaacaaaa gcagctcatc ccttgccttt atcttttccc
     1621 aacttgccaa gttttgagcc aaacagactt tccatgaaag atggcttttc gatctcttcc
     1681 ccagggattc tagaccagtc tgctttttct cgttcgacga gccaactttc cctgccttcc
     1741 ttggcagact ttaatcgctt tacgaaagaa gcatggtctt cattttcaag gtcatcgcgc
     1801 aggtcgagca gactgttgat catcacattc aacgaatgga tggtatttgc gcggttgtgt
     1861 tccgcgagca tggcaagccc ttccaacgaa ttatctaata aggcagaggt ggcggcgaaa
     1921 tatccgcggc tggcagattt tttcacttcg ttccaccctg gctgactcat cgtgccattc
     1981 aataaagcga cagacgccaa gcgtggcagg atgtaggaag aagccatca
//
LOCUS       DAOQFT010000480         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37911_length_2028_cov_6.422200, whole genome shotgun sequence.
ACCESSION   DAOQFT010000480 DAOQFT010000000
VERSION     DAOQFT010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37911_length_2028_cov_6.422200"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..620)
                     /locus_tag="PLR93_11060"
     CDS             complement(90..620)
                     /locus_tag="PLR93_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27553.1"
                     /translation="MTTKLPQLKQDFVESLSQISRFWGFPKGMGAIFAVLYLSPTPLA
                     LDEITVQTGLTKGAVSTEVRTLARMGLVHRSTKLGDRKDYYEAETDFYTSIRSILKER
                     QNSEFDRALRGVRSSLEQLESGAVTGSKEDIAFLRERIKALQDFFDAIDSLTNAVIKL
                     EKLGLSNVSKILNVLK"
     gene            complement(728..1297)
                     /locus_tag="PLR93_11065"
     CDS             complement(728..1297)
                     /locus_tag="PLR93_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27554.1"
                     /translation="MKRTSKWAIGVVVTVTAAFAALQTVSAPTHQTETPPAETESPVL
                     TEVVATQPCGYQWAYQDDLELTAAFDASIKRLDPNASGRAQLFGENCVRQDGTFTFSA
                     LETDFYVRTPVVDLKQEEEFGNWVKHVMEIVLQIPREELAIQYGFVEFHFEKTETEQL
                     AFRVPIKTYIDEAQSKTGAELFQMFYTQP"
     gene            complement(1300..>2028)
                     /locus_tag="PLR93_11070"
     CDS             complement(1300..>2028)
                     /locus_tag="PLR93_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH27555.1"
                     /translation="VAVKTTADGEFIPAEAGVVLPQGGVLQTGEAGRARLDLNPEGTI
                     VRVAPNSSFTLPQIAVVDGAPKTSIELFFGKIFILLNGGSVDVETPSGVASVRGSLLS
                     VSFNPATQRIQATCLEGHCGLVNESGEDVDLVEGESAYIDEDGVLNELAGIDQDEIQA
                     WLDEAPELNQFLNELPDPQDYPEFQEFDVYDFDPSAYFQENPSEADGFYSFDEGTTGS
                     GSDPNEPPATEPPSDDGSGDSSGG"
BASE COUNT          515 a          473 c          488 g          552 t
ORIGIN      
        1 ggatgtaact cacgatcata gtggtgaggg taatagtgag tagcatttga atgagaaaac
       61 ttataatcgt attcatggtt atttcctttt tatttcaaga cattaagaat tttagagacg
      121 ttagaaagac cgagtttttc cagcttgatg acggcgttgg ttaggctgtc tatggcatca
      181 aaaaaatctt gtaaggcttt gatgcgttca cggagaaaag caatgtcttc tttgcttcca
      241 gtgacagcgc cagattcaag ctgttcaagg gaagagcgta ctccgcgtag tgcccggtca
      301 aattcgctgt tctgacgttc tttcagaatg gagcggatcg aggtatagaa atcagtttca
      361 gcctcgtagt aatctttgcg atcaccaagt ttcgtagagc ggtggaccaa acccatgcgc
      421 gccagggttc gtacctcggt gctgacggca cccttggtga gtccggtctg aaccgtaatt
      481 tcatccagtg ccagcggagt aggggagaga tataagacgg cgaagatcgc gcccatccct
      541 ttgggaaagc cccaaaagcg actgatctgg ctcaggcttt cgacaaaatc ttgtttgagt
      601 tgggggagtt tggtcgtcat atattcctcg ttttagaacg tttagtaatt actaaacgta
      661 gtatatgacc gtttgatgag cttgtcaagg acaaaaaaac cgccttcaaa ggcggtttcg
      721 tttttgatta gggttgggtg taaaacatct ggaataactc agcaccggtc ttgctttgtg
      781 cctcatcgat ataggtttta atgggcactc gaaaggcgag ttgttcagtt tcggttttct
      841 caaagtggaa ttctacgaac ccatattgaa ttgccaactc ttctcgcgga atttgcaaca
      901 cgatttccat aacgtgtttg acccaattcc caaactcttc ttcttgcttc aaatctacta
      961 ctggcgtgcg gacgtaaaaa tctgtttcta gagcagagaa agtgaaggtt ccatcttggc
     1021 gtacacaatt ttcgccaaaa agctgggcgc gtccgctggc attggggtct aagcgtttaa
     1081 tagatgcatc aaacgctgcc gtcaattcga ggtcatcttg atatgcccat tgatacccgc
     1141 aaggttgagt agcaacgact tctgtgagaa cagggctttc tgtttcagca ggaggtgttt
     1201 ctgtttggtg ggtaggtgca gagactgttt gtaaggcagc aaaagcagct gtgaccgtta
     1261 ctacaacccc aattgcccat ttgcttgtgc gtttcacagc taccctccgc tagagtcgcc
     1321 gctgccatca tctgaaggag gctcagtagc aggtggctcg ttcgggtcac tgcctgagcc
     1381 ggttgtacct tcatcgaatg agtagaagcc gtctgcttca ctgggatttt cttgaaagta
     1441 tgcagaaggg tcaaaatcat acacatcaaa ttcctgaaac tcagggtaat cttgagggtc
     1501 tggtagttca tttaagaact gattcaattc tggggcttca tccaaccaag cttgaatctc
     1561 atcctggtca atgccagcca actcattgag caccccatct tcatcgatat aagcagactc
     1621 gccttctact agatcaacat cttcaccact ttcattgacg agtccgcaat gaccttccaa
     1681 acaggtagct tgaatgcgct gggtagccgg gttgaaacta acgctcaaca aactgccacg
     1741 cacagaagct acacccgatg gcgtttctac gtcaaccgag ccgccgttca ataagataaa
     1801 aatcttgcca aagaaaagct caatgctggt tttcggcgcg ccatccacta cggcgatttg
     1861 cggcaaggta aaagaagaat ttggcgcaac acgaacgatg gttccttcgg gattcaaatc
     1921 taaacgcgcg cgcccggctt ctccggtttg caacacgccg ccttgtggca gaacaacccc
     1981 tgcctctgcc gggatgaact ctccatctgc agttgtttta accgctac
//
LOCUS       DAOQFT010000481         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_37974_length_2026_cov_6.178590, whole genome shotgun sequence.
ACCESSION   DAOQFT010000481 DAOQFT010000000
VERSION     DAOQFT010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37974_length_2026_cov_6.178590"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="vorB"
                     /locus_tag="PLR93_11075"
     CDS             complement(<1..931)
                     /gene="vorB"
                     /locus_tag="PLR93_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNH27556.1"
                     /translation="MPKELWKGNEAIAESAVRAGLGAYFGYPITPQTEVLEYLSRRMP
                     ELGRAFVQAESELGAINMVYGAACTGVRVMSSSSSPGMSLMMEGLSYIAGTEIPAVLV
                     NVMRGGPGLGNIAPAQSDYNQAVRGGGHGDYPRIVLAPASVQEAIDLTALSFDLAEKY
                     RMITLIILDGTTGQMMEPAEMPAMQEMHRKNFEWATNGAMNRERRVLSSIYLNPLDEE
                     QANLRMLRNWKNVQANEVRYKEYFLDDAKYVVVGFGTAGRVALSAVREARAQGIKVGL
                     LRPITVSPFPYEALEQLIPQAEAFLVAEMNMGQM"
     gene            complement(985..1227)
                     /locus_tag="PLR93_11080"
     CDS             complement(985..1227)
                     /locus_tag="PLR93_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH27557.1"
                     /translation="MAAKGWIEVSDTYCKGCELCISACPQNVMELNMEHLTPKGYHPA
                     HTFKDGCTGCAICAVVCPDAAITVYREVTKAAPVAA"
     gene            1449..1697
                     /locus_tag="PLR93_11085"
     CDS             1449..1697
                     /locus_tag="PLR93_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27558.1"
                     /translation="MENLKEWLLGLDTMILVILFLGGFIAWELKSGEVPLRWFGSIRR
                     DARPFFYWIAMLFHLAILGIVVYAYTDGVRMPLAEFFQ"
     gene            complement(1729..>2026)
                     /locus_tag="PLR93_11090"
     CDS             complement(1729..>2026)
                     /locus_tag="PLR93_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27559.1"
                     /translation="QASTNMTRSVTSEMLPRAMRWMFPIFKLAVREDGGKSAAKAARS
                     SVYLASSPEVDGVTGKYFDTNCKEAAWSPAVMDEANRETLWKIVEQLTFATKEY"
BASE COUNT          473 a          515 c          495 g          543 t
ORIGIN      
        1 gcatttgccc catattcatt tcagcgacga ggaacgcctc agcctgcggg atgagttgtt
       61 ccagcgcttc atacggaaac gggctgacag tgatcggtct taacaaacca accttgatgc
      121 cttgtgcgcg cgcctcgcgg accgccgaga gcgcaacacg ccccgccgtg ccaaagccga
      181 cgacaacata tttggcatcg tcgagaaaat attctttgta acgaacttca ttggcttgca
      241 cgttcttcca gtttctcaac atgcgcaggt tggcttgttc ttcatccaac gggttaaggt
      301 agatcgaaga aagcacacgg cgttcgcggt tcatcgcgcc gttggttgcc cactcaaaat
      361 tcttgcgatg catttcttgc atggcgggca tttcagccgg ttccatcatc tgaccagttg
      421 tgccatcgag aatgatgagc gtgatcatgc gatatttttc agcaaggtcg aaggagagcg
      481 cggtcaagtc aatggcttct tgcacggatg cgggagccag cacaatgcgt ggataatctc
      541 cgtgcccgcc gccgcgcaca gcttggttgt aatcactttg agccggggca atattgccca
      601 agccgggtcc gccgcgcatc acgttgacca acactgccgg gatttctgtt ccggcgatat
      661 aagacaaccc ttccatcatc aggctcatgc ccggtgaaga ggaggaagac atcacgcgca
      721 cgcctgtaca tgccgcgcca tagaccatgt tgattgcgcc aagttcagat tcggcttgaa
      781 cgaaagcacg tcccagttcg ggcatgcggc gcgagaggta ttcgaggact tctgtctgtg
      841 gggtgatcgg gtatccaaag tatgccccca acccagcccg cacagcagat tctgcgatgg
      901 cttcattgcc tttccataat tcttttggca ttcgtttctc catgtaaccg gtaggggcga
      961 ctcgcgagtc gcccctacaa ataattacgc cgcaaccggc gcagccttgg tcacttcacg
     1021 atagacggta atcgccgcat ctgggcagac gaccgcacaa atagcacacc ccgtgcaacc
     1081 gtctttgaac gtgtgcgcgg ggtggtatcc cttcggagtg aggtgctcca tattgagttc
     1141 catcacattt tgtggacagg cacttatgca aagttcacag cctttgcaat aggtatcgct
     1201 gacttcgatc caaccttttg ctgccatcac agctccttat attgagaagt tagacactat
     1261 taggcgactt tcatcgctaa gtctattatg tcgttttcgg aaatcgagga gtagtgtcag
     1321 gcgtcatgta caagcgagta caaaggtcat tcgtttttcg gtcaactaca aggacttaag
     1381 agcgacttga acagacaatt tcagaactct gagatgggta taatttcaaa aaatttagga
     1441 gtgtatctat ggaaaactta aaagaatggt tactcgggtt agacacgatg attttagtta
     1501 ttcttttctt gggtggcttt attgcgtggg aattaaaatc tggtgaagtc cctttgcgtt
     1561 ggtttggttc aattcggcgt gatgcccgtc cattttttta ttggatcgcc atgctgtttc
     1621 atttagcaat tctaggcatt gtggtatatg catatacaga tggtgttcga atgcccttag
     1681 cagaattttt tcaataaaaa agagccgcaa ttgcggctct tttttatctc aatattcttt
     1741 tgttgcgaat gtaagttgtt caacaatctt ccatagcgtt tcgcgattcg cttcatccat
     1801 cactgcaggt gaccacgccg cctctttaca attcgtgtcg aaatattttc ccgtcacgcc
     1861 atccacttcg ggcgatgacg caagatatac tgacgaacgc gccgctttcg ccgcagattt
     1921 acctccgtct tcacgaacag ccaatttgaa aatggggaac atccaacgca tagcacgcgg
     1981 cagcatttcg gacgtcacac tgcgagtcat attggtactg gcttgc
//
LOCUS       DAOQFT010000482         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_38154_length_2020_cov_4.916031, whole genome shotgun sequence.
ACCESSION   DAOQFT010000482 DAOQFT010000000
VERSION     DAOQFT010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38154_length_2020_cov_4.916031"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="PLR93_11095"
     CDS             complement(<1..1623)
                     /locus_tag="PLR93_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27560.1"
                     /translation="MSADKSNITRASELTAANLRSVAKTISAQQLSRLTLPEIEAVVQ
                     LVSKIMPAGNVPGMILSGLARLPGRRIPVQKLQQDVTALFSGVEQILDSAVYGAFFAG
                     PAAVLWGYQNLLKLAGKDPESAFPEGMWQFYVDYALREDTARHMNETHGFDTILAQHN
                     IKLDKVDRLTAWFMAAVACLHQYPALLENEWRERVAISLLEKTLRDAGIESQRAKKIH
                     REWEIKRPYRREADGAQYDYPSYRRKKFDEFLQSRLQTMPELVYAAWEAGFNTALEQG
                     LRAYQRQMSIVAYLDPGPYGETRVPFDLSDAKIGIIHKDSYFLLPVCDEIGNPLDVMT
                     VRAQIASLLNSAFVSPSQISSLAKIKRSALAGLRSKLNPVLVNDLDNLKFAPILISTD
                     THSRALPLSELRQTERGVGSHALTIFDTGESFVFDQSHIFFDGAWGTALSEIMTNEAL
                     SWARYLSLLPSPVAPEMRLYTSLTLQLSPADLDLVQQAPKVMPEVGAETDKIDLKACM
                     SLRKDFKQRNDRIELTINDLLVLYRAVHAATYV"
     gene            1719..>2020
                     /locus_tag="PLR93_11100"
     CDS             1719..>2020
                     /locus_tag="PLR93_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27561.1"
                     /translation="MLHGYRLTQHRRIDLDFSASSLDELTGRLSDGFYTTFSTLAQGT
                     KVLGLKAHFDRLYQPAKELGLHPSADEQTVRKEIVDLAKALLPQESRIRLILTKDTG"
BASE COUNT          528 a          497 c          485 g          510 t
ORIGIN      
        1 gacatacgtc gccgcgtgga cggcacgata taacacgagc aggtcgttga tggtcaattc
       61 gatgcggtca ttccgttgct taaagtcttt gcggagcgac atgcaggctt tcaggtcaat
      121 cttatctgtt tcggcgccga cttctggcat aacctttggt gcttgctgaa caaggtctag
      181 gtcggcggga gaaagctgta acgtgagaga ggtgtacaaa cgcatttcag gggcaactgg
      241 ggaggggagt aagcttagat accttgccca tgaaagggct tcattggtca taatctcaga
      301 aagtgccgtg ccccaagccc cgtcaaaaaa gatgtgagat tggtcaaaaa caaaagactc
      361 gccagtatca aaaattgtca acgcatggga acccacgccg cgctccgttt gacgtaactc
      421 cgaaagtggc aatgcccgcg aatgtgtgtc tgtagagatc agaattggcg caaattttaa
      481 attatccagg tcgttcacca aaaccgggtt aagttttgag cgtagccctg ctaatgccga
      541 gcgtttgatc ttcgctagag atgaaatctg agacggggaa acaaatgcag agttcaataa
      601 agaagcgatc tgcgctcgaa ccgtcattac atccaacggg ttgccaattt cgtcgcagac
      661 gggcagcaag aaataactat ctttatggat aatcccaatt tttgcatctg atagatcgaa
      721 tggcacacgc gtttcaccgt aaggtccggg gtcgagatat gccacaatcg acatttggcg
      781 ttgatatgct cgtaagcctt gttctaacgc cgtattgaag ccagcttccc atgccgcata
      841 caccaactct ggcatggttt gcagtcggct ttgtagaaat tcatcgaact tcttacgtcg
      901 gtaggatgga tagtcatatt gcgcgccgtc cgcttcacga cgataaggac gttttatttc
      961 ccactcacgg tggatctttt tcgcgcgttg tgactctatt ccagcatcgc gcaacgtttt
     1021 ttcgagcaaa gaaatggcaa ctcgttcacg ccattcattt tcaagcaatg ccgggtattg
     1081 atgcaaacaa gcaactgcag ccatgaacca agccgtcagc cggtcaactt tatcgagttt
     1141 gatattatgc tgtgcaagga tcgtatcaaa gccatgcgtc tcgttcatat gacgggctgt
     1201 atcttcacgc agagcatagt ctacataaaa ttgccacatc ccttcgggga acgcagactc
     1261 agggtctttg ccggctaact tcaacagatt ttggtacccc cataaaacag cagcgggtcc
     1321 ggcaaagaac gcgccataca cagcactatc caagatttgt tctacaccgc taaaaagagc
     1381 agttacatct tgttgtaatt tttggacggg gatacgtcga ccgggcaaac gtgccagccc
     1441 gctaaggatc ataccgggca cattccctgc cggcataatc ttggagacca attgcaccac
     1501 cgcctcgatc tctggtaatg tgaggcgtga aagctgttgc gcagaaatgg tctttgcaac
     1561 cgagcgcaaa ttagcagcag tgagctctga agcacgtgta atgttggatt tgtctgcgct
     1621 catctatgaa attatatttc accttcatct tgatggattt ttttgtctcg cctcaatctg
     1681 tttgcaaatt tgttaggcag cggatatata atcagcccat gcttcatgga tatcgcctca
     1741 cacaacacag acgaattgat cttgatttca gcgcctcttc tctcgacgag ttaacgggtc
     1801 ggctttcaga tggcttttac accaccttta gcaccctcgc gcaagggaca aaggtgcttg
     1861 gcttgaaagc tcactttgac aggttatatc aacctgcgaa agaactaggg ctgcatccct
     1921 cagcagacga acaaaccgtc cgcaaagaga tcgtggactt ggcaaaagcg ctcttgccac
     1981 aagagtcccg cattcggctt atcctcacaa aagacacagg
//
LOCUS       DAOQFT010000483         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_38255_length_2017_cov_5.563201, whole genome shotgun sequence.
ACCESSION   DAOQFT010000483 DAOQFT010000000
VERSION     DAOQFT010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38255_length_2017_cov_5.563201"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLR93_11105"
     CDS             <1..488
                     /locus_tag="PLR93_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNH27562.1"
                     /translation="PGLLTPGEAKVGIIPGNIAIPGNVGVVSRSGTLTYEVLYAMKNL
                     GMGASTCVGIGGDPVNGTNFTDVLEMFEHDPKTEKVIMIGEIGGNEEEKAAEFIRTKM
                     TKPVASFIAGQTAPPGKRMGHAGAIIEGGAGTAADKVKALEAAGVKVAKHPEEIPTLL
                     Q"
     gene            complement(595..831)
                     /gene="acpP"
                     /locus_tag="PLR93_11110"
     CDS             complement(595..831)
                     /gene="acpP"
                     /locus_tag="PLR93_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNH27563.1"
                     /translation="MSDTYSELKVIIKDLLGADEEKITMEARFREDLEADSLDLVELI
                     MAFEDKFGAEISDEDAQKITTVGEAVKYIEANKK"
     gene            919..1947
                     /gene="fni"
                     /locus_tag="PLR93_11115"
     CDS             919..1947
                     /gene="fni"
                     /locus_tag="PLR93_11115"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNH27564.1"
                     /translation="MTKVAPIDQRKADHIKINLEQDVRSALTTGLENYHFTHEALPEL
                     DLNRLDTSLKLFGKTLTSPTLVSSMTGGTDEAETINLRLAEAAQECGVAMGVGSQRAA
                     IEHPEQAKTFQVRRVAPDILLFANLGAVQFNYGYGIDQCRRAVDMIQADALYLHINPL
                     QEAVQDAGDTNWVGITKKIEEVCKKLEVPVIAKEVGWGFSEKTAKVLADCGVSAIDVA
                     GAGGTSWSQVEMYRAPDEFTRRLAATFVGWGIPTAEAIQNVKRAAPDMTIFASGGLKD
                     GLDIAKSIALGATLGGMAGEFLKAAAISTERAVEMMKLTKRQIEVTMFTSGAGTLHEL
                     AEKLTKKA"
BASE COUNT          551 a          552 c          467 g          447 t
ORIGIN      
        1 gccccggtct gttgactccc ggtgaagcca aggtcggcat catccccggc aatattgcca
       61 tccccggcaa tgtcggcgtg gtctcccgct ctggcacatt gacctatgaa gtgctctatg
      121 ccatgaagaa cctcggcatg ggcgcatcaa catgtgtcgg catcggtggt gaccctgtca
      181 acggtaccaa cttcaccgat gtgcttgaaa tgttcgaaca cgaccccaag accgaaaagg
      241 tcatcatgat cggtgagatc ggcggtaatg aagaagaaaa agccgctgaa ttcatccgca
      301 ccaaaatgac caaacccgtc gcatccttca tcgcgggtca aaccgctcct cccggaaaac
      361 gcatgggaca cgccggcgcg atcatcgaag gtggagccgg aaccgcagcc gacaaggtca
      421 aagctctcga agctgccggt gtcaaagttg ccaaacaccc cgaagaaatc cctacccttt
      481 tacaataaga ccaaaggggc gactctcatc aagtcgcccc tttcattttc tatcaagaat
      541 ccaaagagcc gccctataca gagcggctct acggttataa tcttgaacag ttaattactt
      601 cttgtttgct tcgatgtact ttacagcttc gccgacagtc gtaatcttct gagcatcttc
      661 atcagagatc tcagcgccga acttatcttc aaacgccatg atcagctcaa ccaggtcgag
      721 cgaatcagct tcgaggtcct cacggaagcg cgcttccatg gtgattttct cttcatcggc
      781 accaagcaaa tccttgatga tcactttcaa ttcagaatat gtgtctgaca taacatcctc
      841 cagttggaat ttattgtaaa ttgtaatcgc atggcttgca ctgtcaagcc agcatatcaa
      901 tcacaggaac acccccttat gacaaaagtt gcgcccatag accagcgaaa agccgaccac
      961 attaaaatca acttagaaca ggatgtccgc tctgcgctga caactgggct ggaaaattac
     1021 catttcactc acgaagccct gccagaattg gatttaaacc ggctcgacac aagcctgaag
     1081 ctgttcggca aaaccttaac ctcccctaca ctggtaagtt ctatgaccgg cggcacagac
     1141 gaagccgaaa ccatcaattt gcgcctcgct gaagccgccc aagaatgcgg agttgccatg
     1201 ggcgtgggaa gtcaacgtgc cgccatcgaa caccctgaac aagccaaaac atttcaagtc
     1261 cgcagagtag cgccagatat tttattattc gccaatcttg gtgccgtgca attcaactac
     1321 ggctacggca tcgaccaatg ccgccgcgcg gtggacatga tccaagctga tgcgttgtat
     1381 ctccacatca acccgttaca agaagccgtg caagatgcag gggatacgaa ctgggtcggc
     1441 attaccaaga aaatcgaaga agtgtgtaaa aaacttgaag tccccgtcat tgccaaagaa
     1501 gttggctggg gcttctctga aaaaacagcc aaggtcttag ccgattgtgg cgtctccgcc
     1561 attgacgttg ctggtgcagg cggcacgagt tggtctcaag tagaaatgta ccgtgcccct
     1621 gatgaattca cacgcagact ggcagctact tttgtcggct ggggaattcc aacagcggaa
     1681 gcgattcaga acgtcaaacg agccgccccc gatatgacca tcttcgcaag tggcgggctc
     1741 aaagacggct tagatatcgc caaatccatc gcccttggtg caacccttgg tggcatggca
     1801 ggtgaatttc tgaaagccgc tgctatttct accgaacgag ccgtagaaat gatgaagctt
     1861 acaaaacgcc agatcgaagt aaccatgttc acttcaggcg cagggacgtt acacgaactt
     1921 gcagaaaagc tcacaaaaaa agcataaaaa tagcggcgtt ttcgccgcta tttttgtaat
     1981 tccaatgggg gatataaatc acgattctct gcttttt
//
LOCUS       DAOQFT010000484         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_38378_length_2013_cov_5.231359, whole genome shotgun sequence.
ACCESSION   DAOQFT010000484 DAOQFT010000000
VERSION     DAOQFT010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38378_length_2013_cov_5.231359"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="PLR93_11120"
     CDS             <1..1808
                     /locus_tag="PLR93_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNH27565.1"
                     /translation="KKAWLDPEGRIISPSRQEHIAKCPLRVGIVSTGLMMRDSLEKQH
                     LLGLRFGVAILDEAHKARSRQGFGKDAGTHNELLAFMREIAARSDHVILGTATPIQTR
                     PEDLWDLIGILHQGSGNFVLGNDFAKWHRPPEVLPILSGELRVTEPSFAWELLRSPLP
                     VVDSTNEQYARRLFSSLRQDLGLINQNDKWDIKNTLSDLSDDSLEYLEDELDRNISGA
                     SFFQRENPFIRHVVLRKRVTLEDAGLLPRIGVDLHPDRELSKEVHQFNSLFEGEALRT
                     SEDFRQAYAEARHFGKALAQSGKGSGFMKNLMEQRICSSIAAGLNTARMLLEGRTVRE
                     ESDEQLIEVKAESPDEIAVLERMIARLERIEEDPKLNAVIHYLEKEKWLKFGVIIFSQ
                     YYDTAKWVADSLAVRYPNQAVGLYAGAGRSRLYQKGDSVNIERETLKRMVAEHQLEIM
                     VATDAACEGLNLQTLGTLINIDLPWNPTRLEQRIGRIKRYGQVREKVDMLNLVNEQTV
                     DEKVYEKLSERMQDRYNLFGSLPDTIRDEWIEDIETMGEKMDEYINAQKQANGFDLRY
                     TETMSPSEKDWRDCSNVLSRRDFAELMTSGWCEGK"
BASE COUNT          542 a          487 c          505 g          479 t
ORIGIN      
        1 aaaagaaagc atggctggat cctgaggggc gaattatttc tccttcaagg caagagcata
       61 ttgccaagtg cccgctgcgt gtaggtattg tatccaccgg gctaatgatg cgcgactcat
      121 tggagaagca gcacctgcta gggttacgct ttggcgtggc tatcttggat gaagcccata
      181 aggcgcgaag caggcaaggc ttcggtaaag atgcaggcac ccacaatgaa ttactcgcct
      241 ttatgcgcga gattgccgcg cgatcagatc acgtcattct tggtacggcc acgcctatac
      301 aaacccgccc cgaggatttg tgggacttga ttggcatcct gcatcaaggc agtggcaatt
      361 tcgtactcgg caacgatttt gccaaatggc atcgcccacc agaggtgcta cctattttat
      421 ccggtgagct gcgcgttaca gaacccagct ttgcctggga actgcttcgc tcaccactac
      481 ctgtcgtcga ttcaactaac gagcagtatg cacgccgctt attcagctct cttcgtcagg
      541 atcttggatt aattaatcag aacgacaagt gggacatcaa gaatacactt tctgatttat
      601 cagatgactc gctggaatac cttgaagatg aacttgatcg aaatatatcg ggtgccagtt
      661 tttttcagcg ggaaaatcct tttatccgcc atgttgtttt acgcaaacgt gtaacgcttg
      721 aagatgcggg gcttctgcca cgcataggcg tcgacttgca tcccgaccga gaattatcga
      781 aagaagttca ccaatttaac agcttgttcg agggtgaagc cctgcggaca agcgaggact
      841 tcagacaagc ctatgcggaa gctcgacact ttggtaaagc attagcccaa agtggtaaag
      901 gcagcgggtt tatgaaaaac ctgatggagc aacgaatttg ctcaagcatc gctgccggat
      961 taaacacagc acgaatgctg ctggaaggcc ggactgttcg tgaagaaagt gacgagcaac
     1021 tcatcgaagt taaagccgaa agcccggatg aaattgccgt tctcgagcga atgatcgccc
     1081 ggcttgagcg cattgaagaa gacccaaagc tcaatgcggt aatccattac ctcgagaaag
     1141 aaaagtggct gaaatttggc gtcattattt ttagccagta ctacgacacc gcgaagtggg
     1201 ttgctgattc acttgccgtg cgttacccca atcaggctgt aggcctctat gccggtgcag
     1261 gacgcagccg cctgtatcaa aagggtgaca gtgtgaatat cgagcgagaa acactgaaac
     1321 gcatggtggc cgagcaccag ctcgaaatca tggtggccac cgatgcagcc tgcgaaggtc
     1381 ttaaccttca aacgctgggc acgctgatta atatcgacct gccatggaac cctactcgct
     1441 tagagcaaag aattggtcgc atcaagcgct atggccaggt gcgcgaaaaa gtcgatatgt
     1501 tgaatctcgt aaacgaacaa accgtcgacg aaaaggttta tgagaagcta tctgagcgaa
     1561 tgcaagatcg ctacaacctt tttggctcac tgcctgatac cattcgagat gaatggattg
     1621 aggatatcga aaccatgggc gaaaaaatgg atgagtacat caacgcccag aaacaggcca
     1681 atggctttga cctgcgctac accgagacca tgtctccgtc cgagaaagat tggcgggatt
     1741 gttccaacgt gctctcacgc cgcgattttg ctgaactgat gacgtccgga tggtgtgaag
     1801 gcaagtagga gcgttgttat tctgacgaat ataacctact ttttaactaa ccaattttga
     1861 ttctggggtt gccatcacta ttggaaagct caacacgaat aaatgctgca agtgatcctt
     1921 cagtccattt ttttaaatcg atgaccgctc caccgcacct cgtcttggcg cgtttaataa
     1981 cttttttccc atcacaggtc acgacagctg ata
//
LOCUS       DAOQFT010000485         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_38605_length_2006_cov_8.102512, whole genome shotgun sequence.
ACCESSION   DAOQFT010000485 DAOQFT010000000
VERSION     DAOQFT010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38605_length_2006_cov_8.102512"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLR93_11125"
     CDS             <1..335
                     /locus_tag="PLR93_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNH27566.1"
                     /translation="RYMGWGEAADLIVKGMEGAIHAKTVTYDFARLMDKPTELKCSEF
                     ASAVINWMDKDIPAAKVAPAKKAVAKKTVAKKVTKKAAPKKAVKKVAKKAAAKKPATK
                     KTAKKKKK"
     gene            complement(403..1107)
                     /locus_tag="PLR93_11130"
     CDS             complement(403..1107)
                     /locus_tag="PLR93_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNH27567.1"
                     /translation="MTSTTPYRKTEIYVALLWIITAAGAIGGASLINPIINAPDYLTL
                     VFPKSVTLTSGMFGWMINDIGIVFIGLLMYPILKKHSESMALGYLSMRMFESLLMIVG
                     VFFALMLIPLSRDFINAGTADVSTLQTIGSILKQAENWFLNLLQLIFLGIGGLILNVI
                     FYKTKLVPRAISIFGFIGYALLLPAAVVGLFGLLDPTPGGPGSILAVPVALWEIIIMP
                     VWLFTKSFNTDYLSTK"
     gene            complement(1121..1288)
                     /locus_tag="PLR93_11135"
     CDS             complement(1121..1288)
                     /locus_tag="PLR93_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27568.1"
                     /translation="MKTKNQKTSGILFIFAGLAFIVAYWLSKQVSFIGVGIAFIGIGA
                     SFIAKSNTVEK"
     gene            complement(1321..1548)
                     /locus_tag="PLR93_11140"
     CDS             complement(1321..1548)
                     /locus_tag="PLR93_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH27569.1"
                     /translation="MEKGKIGNNIRKLRFFRDEMTQQQLAEKVGVTRQTIIAMEQDKY
                     SPSLELAFRIAHVFGVPLEEVFTYQPEDSKE"
     gene            complement(1550..>2006)
                     /locus_tag="PLR93_11145"
     CDS             complement(1550..>2006)
                     /locus_tag="PLR93_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27570.1"
                     /translation="KMSFQEKNIVASLSTFTLILFVYMFRVFQMVQTESFTAPNVFWL
                     WGIVAILAIAGTILLSILTHIAGGIVHKIRTNEDPHIEDIQDERDQLIDLKGTRVAYT
                     VSSLGVAASMLTFVFGQPPLVMFSALIFFGVLAQIVSDGWRLNLYRKGF"
BASE COUNT          553 a          432 c          543 g          478 t
ORIGIN      
        1 tgcgctacat gggctggggc gaagctgccg acttgatcgt gaagggcatg gaaggtgcca
       61 tccacgccaa gaccgtcaca tatgactttg cccgcctgat ggacaagccc accgagttga
      121 agtgctctga attcgcgagt gcggtcatca actggatgga taaggatatt ccagccgcga
      181 aggttgctcc tgctaaaaag gcggtggcga agaaaaccgt tgccaagaaa gtaactaaaa
      241 aggctgcacc gaagaaggca gttaagaaag tagcaaagaa agccgctgct aagaagcctg
      301 caactaagaa aacagcgaag aagaaaaaga agtagttcta gaaataaaag acctcggagg
      361 tttcaaaaac ttccgaggtc tttttcattt gccaagtggt aattattttg ttgaaagata
      421 gtctgtattg aagcttttgg taaacaacca gactggcatg atgatgatct cccagagcgc
      481 cacaggcacc gcgaggatgg agccgggtcc gccgggggtg gggtcaagaa gaccaaataa
      541 tccgacgaca gcggcgggta agagcaaggc atacccaata aagccaaaga tcgaaatggc
      601 tcttggcacg agcttggttt tataaaagat cacgttgaga atcagcccac caatgccaag
      661 aaatatcaat tgcaagaggt tcaagaacca attctccgct tgtttgagga tggaaccgat
      721 agtttgcagt gtagaaacat cggctgtgcc ggcgttgatg aaatctcggc tcaaggggat
      781 gagcatcaac gcgaagaaga cgccgacgat catcaacaaa gactcgaaca tgcgcatgct
      841 taaatatccg agtgccatgc tttcactgtg tttcttcagt atcgggtaca tcaacagacc
      901 gatgaagacg atgccgatgt cgttgatcat ccagccgaac atgccgcttg tgagggtgac
      961 acttttcggg aagactaagg tcagatagtc aggtgcgttg atgatggggt tgatcaggct
     1021 tgcgccgcca atcgctccgg cggcggtgat gatccataat aacgctacgt agatttccgt
     1081 tttacggtat ggagtagttg aagtcattta agaaaatccc ctacttttca actgtgttgg
     1141 atttggcaat gaacgaggct ccgataccaa tgaaggcgat gccaaccccg ataaacgaaa
     1201 cttgcttgct gagccagtac gccacaataa aggcgagccc agcgaagatg aataagattc
     1261 cgctcgtttt ttgatttttc gttttcatgt ttatttgatt ccttttttaa gatgtatggt
     1321 tcattcttta gagtcttcgg gttgataggt aaagacttct tccaagggca ctccgaagac
     1381 atgggcgatt cgaaatgcca actctagaga gggcgagtat ttatcttgct ccatggcgat
     1441 gatggtttga cgggtgacac cgactttttc cgcgagttgt tgttgggtca tctcgtcgcg
     1501 gaagaagcgc agtttgcgaa tgttgttgcc gatcttgcct ttttccatgc tagaagccct
     1561 ttcggtacag gttgagccgc caaccatctg agacgatctg cgcgaggacg ccgaaaaaga
     1621 tcaaggcgct gaacatgacc aggggtggct gaccaaagac aaaggtcagc atggaggcgg
     1681 cgaccccgag cgaagaaacg gtgtatgcca cacgcgtccc ttttaggtcg atgagttggt
     1741 cgcgttcatc ctgaatgtct tcaatgtgag ggtcttcgtt cgtgcgaatc ttatggacga
     1801 tccctccggc aatgtgggtg aggatgctga gaagaatggt tccggcgatg gcaaggatcg
     1861 caacgatgcc ccatagccaa aatacattag gcgcggtgaa actttcggtt tggaccattt
     1921 gaaagaccct gaacatatat acgaacagaa tgagtgtgaa ggtggataac gaagcgacga
     1981 tatttttctc ttgaaatgac attttt
//
LOCUS       DAOQFT010000486         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_38770_length_2001_cov_7.132066, whole genome shotgun sequence.
ACCESSION   DAOQFT010000486 DAOQFT010000000
VERSION     DAOQFT010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38770_length_2001_cov_7.132066"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLR93_11150"
     CDS             <1..1164
                     /locus_tag="PLR93_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27571.1"
                     /translation="LDTDPGDSNYLRPWSDHTTAAYAQKLHEVTRAILDENEESLQVM
                     RRLAAGEEIRLAPASSKGEPDYESRVRLPLKVRFHYMVGNHDWYFHLKGPAFDAIRNE
                     MIQRMGLSNPLNTPFPFDAEEAPELKELLERHKVYARHGDIYDRFNYNREAGRDYGTI
                     GDVFTMDVCNRYPVEVSKRYRDRLPAGVVNSLRKIANIRPLLASPLWISGQIRQFAGN
                     RKLENDLKKVWDEISDEFLQLDFVRQADKAFRFDMVDAMELIVKLSGKASFNTINDVV
                     LWVRKKMWGGKRSFSNYATEEPAFKKGTAQYIVYGHTHSYEIVPLALNLSMPVPESQI
                     YFNSGTWHSYYDLAMQNPKDQLFVPYQALTYLTFYAAEEHDGRQFEVWSGSYA"
     gene            complement(1198..>2001)
                     /locus_tag="PLR93_11155"
     CDS             complement(1198..>2001)
                     /locus_tag="PLR93_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27572.1"
                     /translation="LAGVLVGLSSIILLASLVGMVLAWVYNEPLTLEATTRLDEVETQ
                     LTQIQTDLRKAKDEVERALRIIQSAEDTLSELTQQAGDAKELLEQVNKTLDDDLIPGL
                     EDTRTKLTDVRSILEDLRNALKQLNSLPFVNFEVPGDDVIANIINGVDSLDTEIADVQ
                     GLAKRASTFISDTSYLIGGDFQTTKQNLQDLLVVLKGYDEKLTGWLEQVRMLKESAPK
                     WIDNASLSLTVVLLWFAFSQLGLILHGLSIWYGENPWEVLKKLKKSSVA"
BASE COUNT          508 a          546 c          461 g          486 t
ORIGIN      
        1 ctggatactg accccggaga ctcaaactat ctgagacctt ggagcgacca cacaaccgct
       61 gcgtatgctc agaagttaca tgaagtcaca cgcgccatct tagacgagaa cgaagagtcg
      121 ctgcaagtca tgcgtcggct cgcagccggt gaagagattc ggcttgcccc tgccagcagc
      181 aaaggcgaac ccgattatga aagccgcgta cgccttccgc tcaaagtccg ctttcattat
      241 atggtgggca accacgattg gtatttccat ctcaaaggtc ccgccttcga tgccattcgc
      301 aatgaaatga ttcaacgcat ggggctgagc aacccgctca acacaccctt cccgttcgat
      361 gcagaagaag cacctgaact gaaggaactt ctcgaacggc acaaagttta tgcccgccat
      421 ggcgatattt acgaccgctt caactataac cgtgaagctg gtcgcgacta tggcacgatt
      481 ggtgatgtct tcaccatgga cgtttgcaat cgctaccccg tagaagtttc caaacgctat
      541 cgagaccgtc tccctgccgg ggtggtcaac agcctgcgca agatcgccaa catccgccca
      601 ttgctcgcct ctccactttg gatcagcgga caaatccgtc agttcgccgg gaatcgcaaa
      661 ctggaaaatg acctgaaaaa ggtctgggat gagatttcag atgaattcct gcaattggat
      721 tttgtgcgtc aagccgataa agccttccgc ttcgacatgg tcgatgccat ggagttgatc
      781 gtcaagttat cgggcaaggc ttcttttaac accatcaacg acgtggtcct ttgggttcgc
      841 aaaaagatgt gggggggcaa acggtctttt tccaactatg caacggaaga acctgctttc
      901 aaaaaaggga ctgctcaata cattgtctac ggacataccc atagttatga gattgtgcca
      961 ttggcattga acctgtctat gccggtgcca gagagccaga tctacttcaa ctcaggaacc
     1021 tggcattcgt attatgacct tgccatgcaa aacccaaaag accagctatt tgtgccttac
     1081 caagccctca cctatctaac tttttacgcc gccgaagaac atgatggtcg gcagtttgag
     1141 gtttggtctg ggtcgtatgc ctaaaaaaga aacggtctca aatgagaccg tttcttttta
     1201 tgctacagaa ctcttcttta atttctttaa aacctcccac gggttttcgc cataccaaat
     1261 gctcagcccg tgcagaatca gcccgagctg agagaaggca aaccagagca gaacaactgt
     1321 cagggagaga gaggcgttat cgatccactt aggcgcggac tctttcagca tccgcacttg
     1381 ttcaagccag ccggtcaact tctcgtcata tccttttagc accaccaata aatcttgtag
     1441 attctgcttg gtggtttgaa aatcaccgcc gatcaaatag gatgtgtcac taataaaggt
     1501 agaagcgcgt ttggcaagcc cttgcacgtc tgcgatctcg gtatccaatg aatcgactcc
     1561 gttgatgatg ttggcgatta catcgtcacc cggcacttca aagttgacga agggcagcga
     1621 gttcaactgt ttcaaggcat ttcgcaggtc ttccaaaatc gagcggacgt ctgtcagctt
     1681 tgtcctcgta tcttccaagc cgggaatcag gtcgtcatcc aaagtcttat tgacctgttc
     1741 caacagttcc ttcgcgtcgc cagcctgttg cgtcaactcc gaaagcgtat cctcagcaga
     1801 ctgtatgatc cgcaatgcac gctctacttc atctttcgct ttgcgcaagt cggtttggat
     1861 ctgagttagc tgggtttcca cctcgtctaa ccgcgttgtg gcttcgagag tcagtggctc
     1921 gttgtaaacc caagccaaaa ccatccccac caaactcgcc aacaaaatga tggaactgag
     1981 tccaaccagc acccccgcca a
//
LOCUS       DAOQFT010000487         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_38796_length_2000_cov_7.342416, whole genome shotgun sequence.
ACCESSION   DAOQFT010000487 DAOQFT010000000
VERSION     DAOQFT010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38796_length_2000_cov_7.342416"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLR93_11160"
     CDS             <1..275
                     /locus_tag="PLR93_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNH27573.1"
                     /translation="ILLIGGGIVTTIPLLMFASAAKEIPLNMVGVLQYFAPTIQFLIG
                     VFVYKEPFDHTRLIGFGIVWLALIIFGLENILAHRTPPQTIPELGE"
     gene            278..1276
                     /locus_tag="PLR93_11165"
     CDS             278..1276
                     /locus_tag="PLR93_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH27574.1"
                     /translation="MKLLFIGGTGLISSACSELAIERGHELFLLNRSASKIYPPPKGA
                     TVLQADIYADPAHLSTLLAGQRFDAIVDFIAFHPYDIQRDLSLFRDKTDQFVFISSAS
                     AYQKPVRKYPITEETPLENPFWEYSRNKIAAENLLMDEYRKNGFPVTIVRPSHTYGPS
                     QIPFSYGSWRHQWTLIHRMKQGQPVIMPGDGTSLWVLTWNADFAKGLLGLLGNKKAIG
                     EAFHITSDEALSWDQIYFEMYQALGADSNVVHIPSDYIARFDSEATGTLIGDKSNSVI
                     FDNSKIKRFVPDYHCEVTWREGFRRSLAWFEAHPEFQTIDEDALQNWDRIIQAYTR"
     gene            1410..>2000
                     /locus_tag="PLR93_11170"
     CDS             1410..>2000
                     /locus_tag="PLR93_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNH27575.1"
                     /translation="MNFAMWKKALNVIPEVSKEEWDGLDIISKWLISTRAAVLIMTFI
                     SAALAGLFAWRDGSFSFLPWLALVFGLIMAHASNNIFNDYTDFVRGVDKDNYYRTMYG
                     AQPVASGLMTKRQHLTYFVVTGILAMVAGLYLVWQTEFNTTTWILLGLGSFFVLFYTW
                     PLKGLALGEVAVLMVWGPLMIGGGYYVLTGNWNWSVV"
BASE COUNT          453 a          480 c          471 g          596 t
ORIGIN      
        1 atattctttt gatcggcggc ggcattgtca ccaccattcc gcttttgatg tttgcctctg
       61 cggcgaaaga aattccgctc aatatggtgg gtgtgctgca atattttgca ccgaccattc
      121 agtttctcat cggcgtcttt gtatacaaag aacctttcga ccatactcgt ttgatcggct
      181 ttggcattgt ttggttggcg ctcatcatct ttgggcttga gaatattctt gctcatcgta
      241 ccccgcctca aaccatccct gagttgggcg aataaaaatg aagctccttt ttattggcgg
      301 cacggggttg atcagttcgg catgctctga actcgccatt gaacgcggac acgaactttt
      361 tcttttgaat cgttctgcct cgaagatcta cccgccgccg aaaggagcta ctgttctaca
      421 ggcagatatc tacgctgacc ctgcgcatct ttccacgctc ttggcgggtc agcgtttcga
      481 cgccatagtg gacttcattg cttttcatcc ctatgatatt caacgcgact tatctttatt
      541 ccgcgataag acagaccaat ttgtatttat ttcttctgcc agtgcctatc aaaagccggt
      601 acggaaatat cccattaccg aagagacgcc gctggagaat cctttctggg aatattcgcg
      661 caacaagatt gccgctgaaa atcttttgat ggatgaatat cgcaagaatg gctttcctgt
      721 caccattgtc cgtccttcgc acacttatgg gccttcacaa attccgttca gttatggcag
      781 ttggcgtcat cagtggactt tgattcaccg catgaagcaa ggtcaacccg ttattatgcc
      841 tggcgatgga acctctctgt gggtgctaac gtggaatgct gatttcgcca aaggtcttct
      901 tggcttgctt gggaataaga aagcgattgg tgaagccttt catattacgt cagacgaagc
      961 tctcagctgg gaccaaattt attttgaaat gtatcaagcc ttgggcgcgg actcgaacgt
     1021 ggttcacatc ccatcagact atattgcccg cttcgattcc gaagcgaccg gaacactgat
     1081 cggcgataaa tccaatagtg tgatttttga taatagtaag atcaaacgtt ttgtgcctga
     1141 ttaccattgt gaagttacat ggcgtgaagg gtttcgacgt tcgttggcgt ggtttgaggc
     1201 acaccctgaa tttcagacca tcgatgaaga tgccctgcaa aactgggatc ggatcattca
     1261 agcttatacc cgttaaccgc ttataacact gggggaggtg aggagaacag gtttcatgtc
     1321 attcggtcat aaatcagatg acttttattc taataaatat ggtctgaatt cgttataatt
     1381 ttgagaaaaa aacaaggaga gcaattgtca tgaattttgc aatgtggaag aaagcactta
     1441 atgtgatccc tgaggtctca aaagaagaat gggacggtct ggatatcatt tccaaatggc
     1501 tcatttccac ccgtgcggct gtgctcatca tgacctttat ttctgctgcc ctggcgggtt
     1561 tgttcgcctg gcgtgatggt tcatttagtt ttctcccctg gcttgcgttg gtcttcgggc
     1621 ttatcatggc gcatgccagc aacaatatct tcaacgacta taccgacttt gtccgcggtg
     1681 tggataagga taattactat cgcaccatgt atggagctca acccgtcgct tctggcttga
     1741 tgaccaaacg ccagcacctg acctactttg tggtcaccgg cattcttgct atggttgcgg
     1801 gcttatacct cgtctggcag accgagttca acaccacgac atggattttg ctcggacttg
     1861 gttccttctt tgtcctcttc tatacctggc ccctcaaagg tttggctctc ggtgaagttg
     1921 ccgtcctcat ggtctggggt ccactcatga tcggcggcgg atactacgtc cttacgggca
     1981 attggaactg gtcggtcgtc
//
LOCUS       DAOQFT010000488         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_38883_length_1997_cov_5.792997, whole genome shotgun sequence.
ACCESSION   DAOQFT010000488 DAOQFT010000000
VERSION     DAOQFT010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38883_length_1997_cov_5.792997"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..>1997)
                     /locus_tag="PLR93_11175"
     CDS             complement(267..>1997)
                     /locus_tag="PLR93_11175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNH27576.1"
                     /translation="GHDFRPEYRQIVDVRKKFPNAVCMALTATATSRVRQDIRHTLKF
                     ATTNEFIASFNRENLFIEVVPKQDPAQQVREIIDRYKDQSGIIYCFSRKQVDELAAYL
                     ATKGYSVRPYHAGLDDAERRKNQELFIRDDVQIIVATIAFGMGINKPNVRFVIHHDLP
                     KSIEGYYQEIGRAGRDGLPAHCVLLYSYADVAKLNYFIDQKEGDEKRVAIEHLNAIVR
                     YAEDERNCRRKPLLNYFGESYSAETCSNCDNCNSAPTPLTDVTIPAQKFLSCVKRADE
                     KFGAGHIVDILLGSKSEKVLRWEHDKLSTYGIGKELDRKQWMHLARQLLSMGYLKQEG
                     EFHTLSLTPKALEALRKREPIFGVMQEAERVKKDGRKKAAELDYHRGLFAILRQKRKE
                     MADEAGVPPYVIFSDRTLTEMAAYFPQSSESLLTISGVGQVKAQQYGAPFLEVIREFA
                     EKHKLHEKPKGKQQEKSEVGQRTLLIGELYNGGETVQSLMAKYQVTSQTILDHLTRFV
                     AAGNALQHVTGLEAMTSASEAQKQAVFAAFDEVGTAMLKPVFDKLNGTMNYDDLKVLR
                     LIYMTEQGRQ"
BASE COUNT          496 a          516 c          442 g          543 t
ORIGIN      
        1 aaattgacac ttgccactgg ttgtcacctc ctttccgtta tggaaattgc ccaaaaggca
       61 taaaaaatcg gctgtcttac acagactcag ccgagggttt ccatcaaaaa acctgaaacg
      121 aaccgaatga tcgaacgatc aaaattgcgc ttcaattctc ctttgattac gagcggaacc
      181 gctctgtgcc gaacctgcga gcaggattat acacgattca cccacagatg agaagatgat
      241 taagcggctg gaaactgcgg caagagctac tgcctgcctt gttccgtcat ataaatcagg
      301 cgcaaaacct tcaaatcatc ataattcatt gtgccattca gcttgtcaaa aacaggcttc
      361 aacattgccg ttccaacttc atcaaaagca gcaaacaccg cctgtttttg ggcttctgag
      421 gctgaggtca tggcttccaa acctgtcaca tgttgcaaag catttccagc cgccacaaag
      481 cgcgtcaaat ggtcaagaat cgtctggctg gttacttgat acttcgccat taaactctgc
      541 accgtctcac cgccgttgta cagttcgccg atcagcaggg ttctttgccc cacttcactt
      601 ttctcttgct gtttcccctt tggcttttca tgtagtttat gcttctcagc aaactcacga
      661 atgacctcta aaaatggggc accatactgc tgtgccttca cctgccccac cccagagatc
      721 gtcaataagc tttctgatga ttgcgggaaa tacgctgcca tctcggtcaa agttcggtct
      781 gagaagatca catacggcgg cacccctgct tcgtctgcca tttcttttct tttttgacga
      841 aggatggcaa acagcccgcg atggtaatcc aactctgcgg cttttttcct cccatctttc
      901 ttgacacgtt ccgcctcttg catgacgccg aaaatgggtt cgcgcttccg aagtgcttcc
      961 aacgccttgg gcgtaaggct cagggtatgg aactcccctt cctgcttgag gtatcccatc
     1021 gaaaggagtt gacgagcaag atgcatccac tgctttctat ctaattcttt tccaatccca
     1081 tacgtggaca gtttgtcatg ctcccaacgg agcactttct cacttttaga accgagcaaa
     1141 atatctacaa tatgcccggc tccaaatttc tcgtctgcgc gttttacgca agagagaaac
     1201 ttctgtgcgg gaatggtcac atcggttagc ggagtcgggg cggagttaca gttgtcgcaa
     1261 ttggaacagg tttcggcgga gtatgattcg ccgaaataat tgagcaacgg tttgcggcgg
     1321 caattgcgtt catcctctgc ataacgaaca atggcattta gatgctcaat ggctacacgc
     1381 ttttcgtcgc cttctttttg gtcgatgaaa tagtttaatt tggcaacatc tgcatagcta
     1441 tatagcaaaa cacaatgtgc aggcagacca tcacgcccgg cacgcccaat ttcttggtaa
     1501 tagccttcaa tacttttggg caggtcatgg tggattacaa agcgcacatt aggcttatta
     1561 atgcccatgc cgaaggcaat tgtcgctaca atgatttgaa catcatcacg aatgaataat
     1621 tcttgatttt tacgccgttc tgcatcgtcc agcccggcat gatacggacg gaccgaatat
     1681 ccctttgtgg caaggtatgc agcgagttca tccacttgtt tacgcgaaaa gcagtagata
     1741 atgccagatt gatctttata ccggtcaatg atctcgcgca cttgttgcgc agggtcttgt
     1801 ttgggtacaa cttcaatgaa aagattctcg cggttaaaac tggcgataaa ttcgttcgtg
     1861 gtggcaaact tgagcgtatg ccgaatatct tgccgcacac gagaggtggc tgtcgctgtc
     1921 aacgccatgc aaacggcatt cgggaatttc ttgcgcacat ccacgatctg tcggtattcg
     1981 gggcgaaaat catgccc
//
LOCUS       DAOQFT010000489         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_38908_length_1996_cov_7.558990, whole genome shotgun sequence.
ACCESSION   DAOQFT010000489 DAOQFT010000000
VERSION     DAOQFT010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38908_length_1996_cov_7.558990"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..287
                     /locus_tag="PLR93_11180"
     CDS             75..287
                     /locus_tag="PLR93_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNH27577.1"
                     /translation="MSERIVGTVKWFNATKGYGFIGRDGGEDVFVHFSAIQTDGYRKL
                     EAEQKVEFSIEEGPKGLQAANVVPLQ"
     gene            787..>1996
                     /locus_tag="PLR93_11185"
     CDS             787..>1996
                     /locus_tag="PLR93_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH27578.1"
                     /translation="MKYVVIGTGVAGISAVEAIRSVDRAGEIVMLGDDPHGYYSRPGL
                     AYILTGELQDESLFPKKREDYIPLKFKYKKARVIRIDRANKVLHLDDKTPLAYDKLLI
                     ATGASAIPLTVPGANLQGVMKLDHLEDARGILKYAKRGRTAIVVGGGITALELTEGLL
                     ARGVHVHYLLRGNRYWSSVLDEHESRIVEGRLQAEGVTLHFHSEVQEIIGKSGKVRGV
                     RLLDGRIMKCDMLAYAVGIKPRAELAKSADLALDRGILVNEYLQTNDPNIYAAGDVAQ
                     VYDPLTGRSVLDSLWSPAREQGYAAGLNMAGKSQAYVKMVPFNVTRLAGLTTTIIGAV
                     GHGRDEDIVGIARGDSETWRQLPDAIVAQGGFDINRVRLLVGEDYLIGAVVMGDQKLS
                     VPLQRMISEKI"
BASE COUNT          491 a          439 c          550 g          516 t
ORIGIN      
        1 ccgcgtgccc gacccgcctt tgaaagcgtt taggtggacg ggcataaatt tttatacagc
       61 aggagtaaag aaagatgtca gaacgtattg ttggaaccgt taagtggttc aatgcgacca
      121 aaggctatgg cttcatcggt cgcgacggcg gcgaggatgt gttcgttcac ttcagcgcca
      181 ttcagacaga cggctatcgc aagttggaag ccgaacagaa agtggagttc tccatcgaag
      241 aaggtcccaa aggcttgcaa gctgccaatg tagtgcccct tcagtaagat aataaaatat
      301 ctttgcctaa cagacagggc tgtcgtgaga cagccctgtt ttttatttaa ttttacgaaa
      361 taagactttt aagagaagtt tcatttcttt tcaaactcat ttcatttgta catctttatg
      421 acattcgaca ctatttggat gccctcaggc agatttagta tagatataga aatgccccgt
      481 aactttttca aattctaagc gaccctgtaa atggaggata ttcttgtcca ttcttaatgt
      541 atttaaaacc aacgccagcc ggcatacaac tgcctcatct ggttatgtcg tcccagatcc
      601 gtatcgctgt gtgcaatgcg gtatttgtag ctataactgc cccatcaaca ttgatgtccg
      661 cgcccatgcg tggaggggag agcctgtaaa gcatagtgaa tgtctcactt gtggtgagtg
      721 cgtggcgaga tgtccgcgcg gggtgttacg ctttgaacga acagacttgt ttgccgaagg
      781 tgcctcatga aatatgtcgt cattggtacc ggcgtagcag gcatttcggc agttgaagcc
      841 attcgcagcg tggatagagc cggtgaaatc gtcatgcttg gagatgaccc gcatgggtat
      901 tattcccgcc cggggctggc atacatcctc acaggtgaat tgcaagatga atctcttttt
      961 ccgaagaaac gtgaagatta catcccgcta aagtttaagt ataaaaaagc acgcgtcata
     1021 cgaattgacc gtgcgaataa ggttttgcat ttggatgaca aaacgcctct tgcgtacgat
     1081 aagctgctca ttgcaaccgg agcctctgcc atcccgttga ccgttcccgg tgcgaacttg
     1141 caaggcgtga tgaagctcga ccatcttgaa gatgcgcgtg gaattttgaa gtacgccaaa
     1201 cgcgggcgga ccgccattgt ggtgggaggc ggtatcacag cgcttgagtt aaccgaaggt
     1261 ttactggcgc gcggagtgca tgtccattat ctgttgcgcg gcaatcgtta ctggagcagt
     1321 gtgctcgatg agcacgagtc gcgcattgtt gaggggcgtt tacaagccga aggagtgact
     1381 ctgcattttc actctgaagt gcaagagatc attggcaaat ctgggaaagt gcgtggagtc
     1441 cgtttattgg atggacgcat catgaaatgt gacatgcttg cctatgccgt aggtatcaaa
     1501 cctcgcgcag agcttgccaa gtctgcagac cttgctttgg atcgcggaat tttagtcaac
     1561 gaatatttgc aaaccaatga cccaaatatt tatgcggcgg gcgatgtggc gcaagtctac
     1621 gacccgttga ctgggcgttc tgtgctggat agtttgtgga gccctgctcg cgaacagggg
     1681 tatgctgctg ggttgaatat ggcaggcaag agccaggcgt atgtgaagat ggtgccgttc
     1741 aatgtcacgc gccttgccgg gctgacgacg accattattg gggcagtggg gcatgggcgc
     1801 gacgaagata ttgtgggcat tgcccgtggc gatagcgaga cgtggcgtca actgccagat
     1861 gcgattgtgg cacaaggcgg ttttgacatc aaccgtgtcc gcttgttggt tggcgaagat
     1921 tacctaattg gcgcagtagt gatgggtgac cagaaactct ctgtgccttt gcaaagaatg
     1981 atctcagaaa aaataa
//
LOCUS       DAOQFT010000490         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39094_length_1991_cov_6.350207, whole genome shotgun sequence.
ACCESSION   DAOQFT010000490 DAOQFT010000000
VERSION     DAOQFT010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39094_length_1991_cov_6.350207"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLR93_11190"
     CDS             <1..1025
                     /locus_tag="PLR93_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNH27579.1"
                     /translation="YTIRFKIPQDGVTVAHDHLRGDITIENKQLNDQVILKTDGLPTY
                     HLAAMVDDHEMKITHVMRGSEWLGTFPLHVNIVRAFGWDEPIWIHLSVFLKPSGKGKM
                     SKREAADAMKDGYSIFVKDMQDLGFTPEGVLNWCGLMGWGVAEDDVMTTAQMVDRFTI
                     DSLTPSPAAINFQRLDHFNATHIRLLTNEDLAARLKPYFTREGLAVKDDVLLKMIPLI
                     RERLTTLDDCISFGAFFFKDEVTPTPEDLIAKGLDAKQSVEIAQKAYELLSAQPDISH
                     ERSEPPFREYVEASGFNAGQVFGVLRVAVTGQKVSPPLFESMEIIGRDICLKRIQNAI
                     SILEKM"
     gene            complement(1055..1696)
                     /locus_tag="PLR93_11195"
     CDS             complement(1055..1696)
                     /locus_tag="PLR93_11195"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH27580.1"
                     /translation="MHIQPKDPALQEILLNYSQESDLVKRKKIEDEIWQRYGSANTAF
                     VLDMSGFSLLTRKYGIVHYLSMIRRMQLTVEPIIMGHGGRIIKFEADNCFAVFPEPAA
                     AARTAITIQHALAASNLLTSEDMDVHVAIGIDYGKILILNDDDMYGDAVNRACKMGED
                     IGKADEILISKEAMDMIPEDAQIEGKLIEVSIGGLTMPAYQIVYRTNTEEEQQ"
     gene            complement(1756..>1991)
                     /locus_tag="PLR93_11200"
     CDS             complement(1756..>1991)
                     /locus_tag="PLR93_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNH27581.1"
                     /translation="GAGRKMSRRVAINSISKRDRDDYLKERGVTLLGGGIDESPQAYK
                     QIEDVITAQHDLVDVIGKFTPRIVRMADEPGDI"
BASE COUNT          484 a          495 c          485 g          527 t
ORIGIN      
        1 catataccat tcgttttaaa attccgcagg atggcgtgac agttgcacat gaccacctgc
       61 gcggcgacat tactatcgag aacaaacaac tcaacgatca agttattctc aaaacagatg
      121 gcttgccgac ctatcatctc gctgccatgg tcgatgacca tgaaatgaag attactcacg
      181 tcatgcgcgg ctcagagtgg ctgggcacct tcccgttgca tgtcaacatc gtccgcgcat
      241 tcggatggga tgaaccgatt tggatccatc tttccgtttt tctcaagcca agcggcaagg
      301 gcaaaatgtc caaacgtgaa gcggcagatg ccatgaaaga tggctattcg attttcgtga
      361 aagacatgca agacctgggt ttcacccccg aaggtgtttt gaactggtgt gggctgatgg
      421 gttggggtgt ggctgaagac gatgtgatga ccacggctca aatggtggat cgcttcacca
      481 ttgactcgtt gacgccttct ccggctgcga tcaacttcca gcgcctcgac cacttcaacg
      541 cgactcacat ccgcctcctg acgaacgaag acttggcagc tcgtctcaag ccttacttta
      601 cccgtgaggg gctcgctgtg aaggacgacg ttctgttgaa gatgattcct ctcatccgcg
      661 agcgccttac aactctggat gattgcattt cctttggcgc gtttttcttc aaagacgaag
      721 tcacaccgac tcctgaagac ctcatcgcca aagggctgga tgcaaaacaa tccgtagaga
      781 ttgctcaaaa ggcatatgaa ctcctatctg cgcaaccaga tatcagtcac gaaagaagcg
      841 aaccgccctt ccgcgaatat gttgaagcca gcggattcaa tgcagggcaa gtctttggtg
      901 tgttacgcgt cgcagtgact ggtcagaagg tcagtccgcc gttgtttgag agtatggaga
      961 tcatcgggcg cgatatctgc ctgaaacgca ttcaaaacgc gatttcaata ttggaaaaga
     1021 tgtaaaaaaa gacggagcat ttgctccgtc ttttttattg ctgttcttct tctgtattgg
     1081 ttcgataaac aatttgatac gccggcatgg tcagcccgcc gatgctgacc tcgatcaatt
     1141 taccctcaat ttgagcatct tctggaatca tatccatggc ttcttttgaa atgaggattt
     1201 catcagcttt gccaatgtct tctcccattt tgcaggcacg gttgacagca tcgccataca
     1261 tatcatcgtc gttgagaata aggatttttc cataatcaat gccgatggca acatgcacat
     1321 ccatatcttc actggtaagc aggttagatg ccgctaacgc atgttggatg gttatggcag
     1381 ttcgtgctgc cgctgcaggt tctggaaaaa ctgcgaaaca gttatccgct tcaaatttta
     1441 taatgcgccc accatgcccc ataatgatgg gttcaactgt tagttgcata cggcgaatca
     1501 tggataaata atgaacgatg ccatatttgc gagtgagcag tgagaaacca gacatatcta
     1561 aaacaaaagc agtgttcgcg ctgccgtatc gctgccagat ctcatcttca attttcttac
     1621 gtttcacaag gtcagattct tgcgaatagt ttagtaagat ttcttgcaag gcagggtctt
     1681 tgggttggat atgcataagt tcattatatc gaaaagggtc acgagagtgg cttacattcc
     1741 catgaccctt tttacctaaa tatcgcccgg ctcgtctgcc atgcggacga tgcgcggggt
     1801 gaacttgccg atcacatcca ccagatcgtg ctgggcggtg atgacgtctt cgatctgctt
     1861 gtacgcctgc ggcgactcgt caatgcctcc gccgagcagg gtgacgccgc gctctttgag
     1921 gtaatcgtcg cggtcacgct tgctgatgga gttgatggcg actctgcggc tcatcttgcg
     1981 accggctcca t
//
LOCUS       DAOQFT010000491         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39123_length_1990_cov_6.400000, whole genome shotgun sequence.
ACCESSION   DAOQFT010000491 DAOQFT010000000
VERSION     DAOQFT010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39123_length_1990_cov_6.400000"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /gene="ruvC"
                     /locus_tag="PLR93_11205"
     CDS             <1..446
                     /gene="ruvC"
                     /locus_tag="PLR93_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNH27582.1"
                     /translation="TGYGFVRLMPDGELVAVDYGVILTPKEATASARLVMLFDDLNKL
                     LKKHKPETAAVEKLFFSKNVTTGIAVGQARGVILLALEKAGISTYEYTPNEVKQAVAG
                     YGSAEKRQVQEMVRALLQLEKIPKPDDAADALAIAITHLNTARYD"
     gene            504..1082
                     /gene="ruvA"
                     /locus_tag="PLR93_11210"
     CDS             504..1082
                     /gene="ruvA"
                     /locus_tag="PLR93_11210"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNH27583.1"
                     /translation="MIATLRGEVSHVEDNAIIVEVGGVGMRVFVPAPMRTKASAGEMI
                     FLFTHLVVREDALTLYGFESQSDRDLFNILLGVDGVGPKVALSVLSTLTLETIQRAIF
                     ADEEELLSKVPGVGKKTAQKMAIHLKDKLKPTDALSKMAALADYDSEVLAALAALGYS
                     VVEAQAALQALPKDAPKDVEERLRVALQYIGK"
     gene            complement(1169..1885)
                     /locus_tag="PLR93_11215"
     CDS             complement(1169..1885)
                     /locus_tag="PLR93_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27584.1"
                     /translation="MQSQNEFNESSVSIQSFLLFLFASVIGLLVATLVLPFVLPNMAF
                     SLSGASPKAYWYLSRGTAFVSLSLLWLSMALGLGITNKMARLWPGMPATFAIHEYVSL
                     LGLAFAAFHAVILLGDEYINFTLLQVFIPFSTVDYRPFWVGIGQLGFYIWLIVAMSFY
                     VRPMIGQKFWRVLHYLSFAMYFVGIFHAIFSGTDSSMSWAQNYYWFSAGSLLFLFFVR
                     IVSIVIDGLFPVKKPVPVQR"
BASE COUNT          580 a          495 c          485 g          430 t
ORIGIN      
        1 ccaccggcta cggattcgtg cgcctcatgc cagacgggga actggtcgct gttgattatg
       61 gcgtgatcct caccccgaaa gaagccaccg cctccgctcg cctggtgatg ctttttgacg
      121 atctcaataa gcttcttaaa aaacataaac ccgaaactgc tgccgtagaa aaattatttt
      181 tctcgaagaa tgtcaccacc ggcatcgcgg tgggacaagc ccgcggcgtg attttgctgg
      241 cacttgagaa agccggcatt tctacctacg aatacacccc aaatgaagtc aaacaagcgg
      301 tagcggggta cggctccgct gagaagcgac aagtgcaaga aatggtgcgc gctttattgc
      361 agttagagaa aatccccaaa cctgacgatg ccgccgatgc attagcaatt gccatcacgc
      421 atttgaatac agctcgatat gattaattat gtaggggcga ctcgcgagtc gcccctacat
      481 tttatttacg gtaaaataca aacatgattg ccaccttacg cggagaagta tcacacgttg
      541 aagataatgc catcatcgtc gaagtaggcg gtgtgggcat gcgagtcttc gtcccggcgc
      601 cgatgcgcac aaaggcttcg gcgggagaaa tgatcttttt gttcacccat ctcgtcgtcc
      661 gggaagatgc gctgactctt tatggcttcg aatcccaatc agaccgcgac ctcttcaaca
      721 tcttgctcgg cgttgatggc gtgggtccca aagtggcgct ctcggtgtta tccacgctca
      781 cgttggaaac cattcaacgt gccatctttg ccgacgaaga agaattgctt agcaaagttc
      841 cgggtgtggg caaaaagact gcgcaaaaaa tggcgatcca cctcaaagac aaacttaagc
      901 cgaccgatgc gctttccaaa atggcagctc ttgccgacta cgatagcgaa gttctcgccg
      961 cgcttgccgc attgggttac tcggtggtcg aagcgcaagc cgcattacaa gcgcttccca
     1021 aagatgctcc caaagatgtc gaagaacgtt tgcgcgtggc gttgcaatac atcggcaaat
     1081 aatggctcta ctatttggat aaggtctaaa tcaaaagaga ctgtcacggt gaaagtgaca
     1141 gtctctttat ttggcatgtt gttcaaatct atcgttgaac tgggacgggt ttcttgacgg
     1201 ggaataagcc atcgatcaca atgctgacga tgcgcacaaa gaataagaag agcaaactgc
     1261 ctgcagagaa ccaataatag ttctgtgccc aactcatgct ggagtctgtt ccgctgaaga
     1321 tggcgtggaa aatacccaca aaatacatgg cgaaactcaa gtagtgcagc acacgccaaa
     1381 acttctgccc gatcatggga cgcacataaa aactcatcgc cacgatcaac caaatataga
     1441 acccaagctg accgatcccc acccaaaacg ggcggtaatc tactgtgcta aatgggatga
     1501 atacttgtaa gagggtgaag ttgatgtatt catcgcccag caaaatcaca gcatgaaagg
     1561 cggcaaatgc cagccctaac aaacttacat attcatgaat ggcaaaggtg gcaggcatac
     1621 cgggccacaa acgggccatt ttattggtga tgcctaaccc aagcgccatg gaaagccaca
     1681 aaagagagag tgaaacaaaa gctgtgccgc gcgaaagata ccaatatgct ttgggtgaag
     1741 ctccgctcag agagaatgcc atattgggca acacaaaggg cagaaccaag gtggcgacca
     1801 acagtccaat gacagaggcg aagaggaaca gcaaaaacga ttggatgctg accgacgact
     1861 cattgaattc attttgcgat tgcatgttta actccttgca aatattcagt catggaagag
     1921 gtgataaaca cttcgccaga gtccatgacc attaaaccag cgaaagaagg ctgatcttct
     1981 aaccaagcca
//
LOCUS       DAOQFT010000492         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39148_length_1989_cov_6.007756, whole genome shotgun sequence.
ACCESSION   DAOQFT010000492 DAOQFT010000000
VERSION     DAOQFT010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39148_length_1989_cov_6.007756"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLR93_11220"
     CDS             complement(<1..430)
                     /locus_tag="PLR93_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27585.1"
                     /translation="MSTSLAQVIVLLFIGVFYLPVIFFAIQRREEGQGAATWLVVLYA
                     LLAMVINIAEAIWQDRGDNAFLFQELQTYISFTLIIVLMITIQVFLKRELWWAWLAYF
                     GVWVIGLALILTNALNLPDTLWTNGELILYRSRLGPAWVML"
     gene            484..>1989
                     /locus_tag="PLR93_11225"
     CDS             484..>1989
                     /locus_tag="PLR93_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNH27586.1"
                     /translation="MLTSRASRLKYNFSQDFFRPDGHVVFSDISSARAFANQMSALRT
                     APVSASDLYVISLLDEAYHILLRNYYSRYTGVMGRAMASLQSTFGSKYDLTLNKFTEE
                     FPPKAVFRGETTAGVYLATKLPNFGDFGHGVRAAAIEEMLLINNANKNPALAPYMDLF
                     DENVMNGSAFKDFTSHLLEFFSTQPGLGNGAAGKTETLAELLRGPIEASPDSLEGQLK
                     FILDKWGYLLGNEFSNRILRGMDYLREDVIRKQGPADTFNADVPVPTFTGHEYAEFER
                     YSPDQEWMPRLVLIAKNTYVWLDQLSKKYEREIKNLNDIPDEELDQLAARGFTGLWLI
                     GLWERSRASQRIKQRMGQEDAVASAYSLHSYDIANDLGGWNALQDLRSRAWQRGIRLS
                     ADMVPNHMGIDSQWVIEHPDWFLSLPFSPYPSYSFKSENLSSDLRVGIYLEDHYYDKT
                     DAAVVFQRRDLQTGDLRYIYHGNDGTSFPWNDTAQLDYSNPVVREAVIQVIL"
BASE COUNT          494 a          501 c          460 g          534 t
ORIGIN      
        1 ccaacatgac ccaagcagga cccaaccgcg aacgatacag gatcaattca ccattggtcc
       61 ataacgtatc tggcaggttc aacgcattgg taaggatcaa tgccagccca ataacccaaa
      121 ctccaaaata tgccagccaa gcccaccaga gttcacgctt caagaagacc tgaattgtga
      181 tcatcaaaac aatgatgagg gtgaacgaaa tatatgtttg tagttcttgg aataagaaag
      241 cgttatcccc gcggtcttgc caaatggctt cggcaatgtt gattaccatt gccaataatg
      301 catacaatac gaccagccat gtcgctgcgc cttgcccttc ctctcggcgt tgtatggcaa
      361 aaaagatgac tggcagatag aacacgccaa tgaacaatag gacgatgact tgggctagcg
      421 atgtgctcat gatgatgcct gaaattttac tctaattcat tattaatcgg ttatcattat
      481 tgtatgctaa cttcacgtgc ctcccgtctc aaatacaatt tttcgcaaga cttttttcgc
      541 cccgacgggc atgttgtttt ttcggatatt tcctccgctc gggcatttgc aaaccagatg
      601 tctgcgcttc gcactgcccc cgtttctgcc tctgacctct atgtgatttc tctgctcgac
      661 gaagcctatc acatcttact ccgcaattat tacagccgtt acacgggtgt gatggggcgg
      721 gcgatggctt ccttgcagtc tacattcggc tcgaaatatg accttaccct gaataaattt
      781 accgaagaat tcccgcccaa agcagtcttc cgcggtgaga cgacagcagg agtgtacctg
      841 gcaaccaagt tgccaaactt tggggatttt gggcatggtg ttcgtgcggc agcaatcgaa
      901 gaaatgttgt tgatcaataa cgccaacaag aacccggcac tcgctcccta tatggatttg
      961 ttcgatgaaa acgtgatgaa cggctctgcc ttcaaagatt tcacgtcgca ccttttagag
     1021 ttcttctcca ctcagcctgg tttgggcaat ggcgcagcgg ggaagactga aacccttgcc
     1081 gaacttttgc gcggacccat tgaagcctcc ccagactcat tggaaggtca gcttaaattc
     1141 attctcgata aatggggata tttgcttggt aatgagtttt caaatcgtat tttgcgcggc
     1201 atggattatt tgcgcgaaga tgttatccgc aagcagggtc cagcagatac gttcaatgca
     1261 gatgttcccg tcccaacctt taccgggcat gagtatgcag agtttgaacg ctacagccca
     1321 gaccaagaat ggatgccccg tcttgttctg atcgcgaaga atacctatgt ttggcttgat
     1381 caactttcca aaaagtatga acgcgagatc aagaatctca atgacatccc agatgaagaa
     1441 ttagaccagc tcgcagctcg cgggtttact ggtttgtggc tcattggctt atgggagcgc
     1501 agccgcgcct cacaacgtat caaacagcgc atgggtcagg aagatgctgt cgcttcggcg
     1561 tattctcttc attcgtatga tatcgccaat gatcttggcg gttggaatgc gttgcaagat
     1621 ttgcgctcac gtgcttggca gcgcggcatt cgtctgtctg ccgatatggt tccaaaccac
     1681 atgggcatcg actcgcagtg ggtgatcgaa catccagatt ggtttttgtc cctgcctttt
     1741 tcgccctatc cttcctattc gttcaagtct gaaaatcttt caagtgacct gcgtgtggga
     1801 atttaccttg aagaccatta ttacgataag accgatgctg cagtggtctt tcaacgccgc
     1861 gatttgcaaa ccggcgattt gcgctacatc taccacggca atgacggcac ctccttccca
     1921 tggaatgata cagcccaatt agattacagc aatccggtcg ttcgtgaggc ggtgattcag
     1981 gttatcttg
//
LOCUS       DAOQFT010000493         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39212_length_1987_cov_6.618012, whole genome shotgun sequence.
ACCESSION   DAOQFT010000493 DAOQFT010000000
VERSION     DAOQFT010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39212_length_1987_cov_6.618012"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLR93_11230"
     CDS             complement(<1..847)
                     /locus_tag="PLR93_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH27587.1"
                     /translation="MALITTSNLTKSYGATDIFTGLTVSIEKGERLGIVGPNGVGKTS
                     FLRILAGEDESSSGSVTRTRGVRTGYLSQEADFKMEGTLWDACHSVFDDLIRQQKELH
                     RLEELMATDPDVIEQYGKMQEDFERRGGYTYEIRMKQVLSGLGFDASDYSLSLDHLSG
                     GQRTRGFLARLLLSNPDVLLLDEPTNHLDIRAVEWLEGYLNQWDGAAIIVSHDRYFLD
                     KVSTEILEMLPGAYEIYKGNYKAYLKQREERIARRQEVFESEKEKLLKEVDYIKKHIA
                     GQNTLQ"
     gene            1020..1532
                     /locus_tag="PLR93_11235"
     CDS             1020..1532
                     /locus_tag="PLR93_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27588.1"
                     /translation="MYNEPQNSAISEDRSSSRKLIITIVAVLLFGCCCLVTGVGGYYG
                     YQAYVAAKEVVDQAKEIYEIPVNPDDPNSPNVPIPFTPFSEAPKGGLGDERAREFVWT
                     QIPVYYQEANCPSPKADTTEITVVTQPNAEGSWQEEWKVDCGNGTFHTFVVDFVTQDG
                     FSSGIVQPPQ"
     gene            complement(1610..>1987)
                     /locus_tag="PLR93_11240"
     CDS             complement(1610..>1987)
                     /locus_tag="PLR93_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HNH27589.1"
                     /translation="EVFVEDESYLDMATALSGSGPAYVFLFTEALIDAGVHMGFPRRI
                     AEQLVLQTIKGSASYYQGAARHPATLRNQVTSPGGTSAEALYYLEKAGFRTAISRAVW
                     AAYQRSLELGKEKPGHVEVDEEK"
BASE COUNT          487 a          482 c          499 g          519 t
ORIGIN      
        1 cttgcaaggt gttctgcccg gcaatgtgtt ttttgatgta atccacttct ttgagcagtt
       61 tttctttttc gctctcgaac acttcctgtc tgcgggcaat gcgttcttca cgttgtttca
      121 gatacgcttt gtaattgccc ttgtatatct catacgcgcc gggcagcatt tccaaaattt
      181 cggtgctgac cttatccaaa aaataacggt catgcgaaac gatgattgcc gcaccatccc
      241 attgattcaa atagccttcc agccattcca ccgcgcggat gtcgaggtgg ttggtgggtt
      301 catcgagcag gagcacatcg gggttcgaga gcaagaggcg ggcgagaaag cctcgagtgc
      361 gctgcccgcc tgagaggtga tccaaagaca ggctgtagtc tgaggcgtcg aatcccaaac
      421 cagaaagcac ctgcttcatg cgaatctcgt aagtgtagcc gccgcgtcgt tcaaaatcct
      481 cttgcatctt gccgtattgt tcgatgacgt ctgggtcggt tgccatcaat tcttcgaggc
      541 ggtgcaattc tttttgctgg cggatcaaat catcgaagac ggaatgacac gcgtcccaaa
      601 gcgtgccttc cattttgaaa tcggcttctt gcgacaaata cccggttcgg actccccgtg
      661 tgcgggtcac tgaacccgag ctggattcat cctccccggc aaggatgcgc aaaaagctgg
      721 tcttgcccac gccgttcggg ccgacaatgc cgagccgttc tcccttttca atggagacgg
      781 tcagcccggt gaaaatgtca gttgcgccgt aggatttggt gaggttagag gtggtaatga
      841 gtgccatgag ttttaatgca tagtaaaaat gcgggtggat tttaacccat ctgcgtttgg
      901 tttgttgaaa atgcgtagat tgggattgca agaaaaattg ataaaaccaa agggggatta
      961 aggttttggc tcttttggag ataatacaca ttaattattg tttcaggaga tgtttataaa
     1021 tgtataacga gccccaaaat tctgccatca gtgaagacag aagttcaagc cgaaagctaa
     1081 ttattactat tgttgcagtt cttctgtttg gttgttgttg tcttgtcacg ggggtgggcg
     1141 gctactatgg ctaccaagcc tatgtggcag caaaagaagt ggtagaccaa gccaaggaaa
     1201 tctatgagat tccggtcaac cccgatgacc ccaacagccc caatgttcca attccgttca
     1261 ccccttttag cgaagcacca aaaggcgggc ttggagatga acgagcgcgt gaatttgtat
     1321 ggacacagat tcctgtttat tatcaggaag caaattgccc ctctcccaaa gcggatacga
     1381 ctgaaatcac agtggtcacc cagcctaatg ccgaaggctc atggcaggaa gaatggaaag
     1441 tggattgcgg taatggtacc ttccatacct ttgtcgtgga ttttgtaacg caggatggat
     1501 tttcttcggg aattgttcag ccacctcagt aattactata tgtgaaataa aaacgcggat
     1561 agatgttcat ctatccgcgt tttgtttgaa gtaacctaat tacgaaattc tacttctctt
     1621 catccacctc aacatgaccc ggcttttcct tgcccaattc cagtgagcgt tgatacgccg
     1681 cccaaacggc acgcgaaatc gccgtgcgga accccgcctt ctctaaataa tacaatgcct
     1741 cagcagaagt cccaccgggc gaagtgactt gattgcgcaa ggtggcagga tgccgcgccg
     1801 cgccttgata gtacgaagct gagcctttga tggtctgcaa caccagttgc tctgcaatgc
     1861 ggcgcggaaa acccatgtga actccggcgt ctatcagtgc ttcggtgaag agaaagacat
     1921 atgcgggtcc cgaaccggag agcgcggttg ccatgtcgag atagctttca tcttccacaa
     1981 acacttc
//
LOCUS       DAOQFT010000494         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39280_length_1985_cov_4.990674, whole genome shotgun sequence.
ACCESSION   DAOQFT010000494 DAOQFT010000000
VERSION     DAOQFT010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39280_length_1985_cov_4.990674"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PLR93_11245"
     CDS             <1..1066
                     /locus_tag="PLR93_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNH27590.1"
                     /translation="QTALERKLPPKTDYGIAFVGCGGIVNYGHIPSYRASGFNLIGGY
                     DINQEAAEKTVQTHGMKKVYKSLDDILADPEVQIVDIAVLPEDQWNIARTVIAAEKSL
                     LCQKPLSTKYAQAVEMTNLAQQAGLKMAVQQQFRWSSIIQATRALLNQGWLGDILDVQ
                     VQVSIHTPWDMWPWIANQPRLEVMFHSIHYLDSLRYLFGEPALVTSRHTKNPAQKAKA
                     ETKTVTTWDYASGMQILIAICHYDWSPALYSNFRVLGTDGVIEGTIGTNYDYPDGRND
                     TLKFTSREHSEQNFSATLPGKWIPDAFYGPMASLMEAIQTGSEPVTSARDNLGTLRVV
                     EAAYRSMKEAHSVSPLEITA"
     gene            1164..>1985
                     /locus_tag="PLR93_11250"
     CDS             1164..>1985
                     /locus_tag="PLR93_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNH27591.1"
                     /translation="MPLTPERLQDAYRQMLVIRKFEEAIRSLYQQGKIRGSFHPCVGQ
                     EATAVGGCWPLRKDDYLTCTYRGHGQAIAKGLSVRAAMAEMLGKATGCSKGKGGSMHF
                     TDPSVGLLGANAIVAGGVPHAAGAALSSRLQKKDNVAIAYFGEGAVNQGVFLESLNLA
                     VVWKLPLILVCENNLYSEMSPSHETTSVVETYKRAEGFGFEAVQIDGNDVEAVYNTVE
                     TVAGKARAGGGPFYIEAMTYRLWGHMMGDPEIYRTKEEVAKAKENEPIVRCGKRLI"
BASE COUNT          518 a          470 c          544 g          453 t
ORIGIN      
        1 tcaaacagca ttggagcgga aactgccccc aaagacagat tatggcatcg cctttgttgg
       61 ttgtggcggg atcgtgaatt atggtcacat cccttcctat cgcgccagcg gcttcaatct
      121 aattggcggg tacgacatca atcaggaggc ggctgaaaaa acggttcaaa ctcacggcat
      181 gaaaaaagta tacaaatcgc tcgacgatat actggcagac cccgaagttc agatcgtgga
      241 tattgccgtg ctgccggaag atcaatggaa tattgccaga actgtgattg ccgcggaaaa
      301 atccttactc tgccagaagc ctctttccac gaagtatgcg caagcagtgg agatgacaaa
      361 tctggcgcag caagccgggt tgaaaatggc ggttcaacag caatttcgtt ggagttccat
      421 tattcaagcc acacgcgcgc tcttaaatca gggctggctg ggcgatattc tcgacgtgca
      481 agttcaagtc agcatccaca ctccctggga tatgtggccc tggattgcaa accagccgcg
      541 ccttgaagtc atgttccatt ccattcatta tctcgactcc ctgcgttatc tcttcggcga
      601 gcctgcactt gttacaagcc ggcatacgaa gaatccagcc caaaaagcaa aagcggaaac
      661 gaagacagtc accacctggg attatgcgag tggaatgcag attctgatcg ccatctgcca
      721 ctacgactgg tcaccggcgc tttattcgaa tttccgcgta ctcggcacag atggcgtgat
      781 cgaagggacg attggcacca attatgacta ccccgatggg cgcaatgaca cgttgaaatt
      841 cacaagtcgg gaacattcgg aacaaaattt ttcagccacc ctgccgggta aatggatacc
      901 ggatgctttt tacggaccga tggcttccct gatggaagcg attcaaactg gcagcgaacc
      961 tgtcacgtcg gcaagggaca acctcggaac tctgcgcgtt gtagaagcgg cttatcgttc
     1021 gatgaaggaa gctcacagcg tgagtccatt ggaaatcaca gcatagattc aaggcggaac
     1081 gtcacgaagg ttctcgcaaa taaggctggt ttatacctaa aaccttcggg acggtgcgtt
     1141 actaaatttt aggagatggt tacatgcctt tgacacctga aagattgcaa gatgcctatc
     1201 ggcagatgct tgtgatccga aaatttgaag aagccattcg cagcctgtat caacaaggaa
     1261 agattcgcgg ctcgtttcac ccctgtgttg ggcaggaagc gactgcagtg ggtggctgtt
     1321 ggccgttgcg aaaagatgat tatttgacat gcacctatcg tggtcatggt caggcaattg
     1381 ccaagggact gagtgtccgt gcggcgatgg cggaaatgct gggcaaggca acgggttgct
     1441 ccaaaggcaa gggcggctcg atgcacttta ccgatccttc ggttgggttg ctgggagcca
     1501 atgcaattgt ggccggtgga gttccacacg cggctggggc agcgctctcc tctcgattgc
     1561 aaaagaaaga taatgttgcg attgcatact ttggagaagg ggcggtcaac cagggtgttt
     1621 tccttgaaag cctgaacctc gctgtcgttt ggaaattgcc gctcatcctc gtatgtgaaa
     1681 acaacttgta ttccgaaatg tcgccttctc atgaaaccac ctcggtggtg gaaacatata
     1741 aacgcgcaga gggcttcggt tttgaagcgg ttcaaattga cgggaacgat gttgaggcgg
     1801 tgtacaacac tgtcgaaaca gtggcaggca aggcacgggc aggaggcggt ccgttttaca
     1861 tcgaagcgat gacctatcga ctgtggggtc acatgatggg cgacccggaa atttatcgta
     1921 cgaaagaaga agtggcaaaa gcaaaagaaa atgagcccat cgttcgttgc ggaaaacggt
     1981 tgatc
//
LOCUS       DAOQFT010000495         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39353_length_1983_cov_4.883817, whole genome shotgun sequence.
ACCESSION   DAOQFT010000495 DAOQFT010000000
VERSION     DAOQFT010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39353_length_1983_cov_4.883817"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..851
                     /locus_tag="PLR93_11255"
     CDS             120..851
                     /locus_tag="PLR93_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27592.1"
                     /translation="MKKLIFIGILLVLSACNLPVTADSSSTQAPLTTPETADTTQAVL
                     PTETIAPTATSEPFPQFFIDQFDSESPYWEFMQAGGLSAVTPTFSNRTLHFDIPATDT
                     WMIGIHSANSYENVFVRATTSVSPTGSAGLICRYSEDGWYEFNVATDGAYSLLLGQWL
                     SDGVVKYIPVINDVSNKLTELTNTEIGMFCQDNFIQLYVNSTMINRAEVTNYGLTEGK
                     IGISAASFIEAPNFVLFDQVQVSDQ"
     gene            942..>1983
                     /locus_tag="PLR93_11260"
     CDS             942..>1983
                     /locus_tag="PLR93_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNH27593.1"
                     /translation="MGKIIGIDLGTTNSVVSVMEGGSPTVITTAEGGRLCPSVVAFNK
                     NGERMVGQTAKRQAVVNADNTIYSIKRFIGRHFDETASERAMVPYQVAQGPTGDVRVK
                     IPVNGKEYSPQEISAMVLSKLKADAEAYLGEPVTQAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTASALAYGLDKKKNETILVFDLGGGTFDVSILEVGEGVIEVKATNG
                     DTHLGGDDWDQSITNWAADEFKKDQGIDLRQDRQALQRLREAAEKAKIELSTVMETEI
                     NLPYITADASGPKHLQLKLSRSKFEQMTEDLLQRCRKPFEAALKDAGMDASKLDEVVL
                     VGGSSRMPMVQDL"
BASE COUNT          549 a          491 c          461 g          482 t
ORIGIN      
        1 atctttcgga cccgggttca attcccggca tctccaccta catcattgcc cagtatgcac
       61 tgggcaatga ttgtttataa aagcatcccc actaaataac ttatgatata aaatgcagca
      121 tgaaaaagtt gatttttatt gggattctcc tcgtactttc tgcctgtaat ttgccagtaa
      181 cagcggattc atcctctacg caagcgcctc ttaccacgcc ggaaactgct gacacaaccc
      241 aagcagttct gcctacggaa acgattgctc caacagcaac ctctgaacca ttccctcaat
      301 tttttataga tcaattcgac tctgaatcgc cctactggga atttatgcaa gcaggcgggc
      361 tctccgccgt cactcccacg ttttcaaaca ggacactcca ctttgatatc ccagctaccg
      421 atacatggat gattggtatt cacagtgcca acagctatga aaatgttttc gtacgtgcta
      481 caacttcggt ttcaccaaca ggctctgccg gtttgatatg ccgatattct gaagatggct
      541 ggtatgagtt taatgtcgct acagatggag cgtatagcct tcttctcggg caatggcttt
      601 cagatggggt cgttaaatac attcccgtca ttaatgacgt cagcaataaa ttgacagaat
      661 taacaaatac cgaaatcggc atgttttgcc aagataactt tatacaactc tatgtaaata
      721 gcaccatgat taaccgtgca gaagtaacca actacggtct cactgaagga aaaattggaa
      781 tttcagctgc atcgtttata gaggctccaa actttgtact tttcgaccag gtacaagtta
      841 gcgaccaata agtttatcta aaacctatag attgttaaca gaattcttgc actcgtcgct
      901 taatatgggg acattgaaaa agagtaacaa ggagatttct catgggcaaa atcataggaa
      961 ttgacctcgg cacgaccaac agcgtcgtct ccgtgatgga aggcggctct cccacagtca
     1021 tcaccacagc agaaggcgga cgtttgtgtc catctgttgt ggcgttcaac aagaatggtg
     1081 agcgtatggt cgggcagacc gccaaacgtc aggctgtggt caatgcagat aacacaatct
     1141 attctattaa gcgctttatt gggcggcatt ttgatgagac cgcctcagag cgtgctatgg
     1201 ttccttatca agtggcgcag ggacccacag gcgacgtgcg cgtcaagatc cccgttaatg
     1261 gcaaggaata ctcaccccaa gaaataagcg caatggtgtt gagcaagctc aaggcggatg
     1321 ctgaggcgta ccttggtgaa cccgtcactc aggcggtcat taccgtgcct gcttatttca
     1381 acgatagcca acgtcaagct acaaaagacg ctggcaagat cgcaggcttg gaagtgttgc
     1441 gcatcatcaa cgaaccgacc gcttctgcgt tggcatacgg gctcgacaaa aagaaaaatg
     1501 aaactatcct cgtctttgac ttgggcggtg gtacgttcga cgtatccatc cttgaagtag
     1561 gcgaaggcgt aatcgaagtg aaagccacca atggtgacac tcacctcggc ggtgacgatt
     1621 gggatcaaag catcaccaat tgggcagccg atgaattcaa gaaagaccaa ggtattgacc
     1681 tgcgccagga tcgccaagca ttacaacgtt tgcgcgaggc agcagaaaaa gccaagatcg
     1741 aactctcgac cgtgatggaa acagagatca acctgccgta catcactgcc gatgcttcag
     1801 gtccgaaaca cttgcagttg aaactcagcc gctctaaatt tgagcaaatg accgaagacc
     1861 tgctccagcg ttgccgcaag ccgttcgaag ccgcgctcaa agatgcaggc atggacgcca
     1921 gcaagcttga cgaagtcgtt ttggtcggtg gttcttctcg catgccgatg gtgcaggact
     1981 tgg
//
LOCUS       DAOQFT010000496         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39448_length_1980_cov_6.729351, whole genome shotgun sequence.
ACCESSION   DAOQFT010000496 DAOQFT010000000
VERSION     DAOQFT010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39448_length_1980_cov_6.729351"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLR93_11265"
     CDS             <1..605
                     /locus_tag="PLR93_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27594.1"
                     /translation="LGVDLALESAFFGVLMLVCVLFVPFWLWLSKRQNKIRAYILGMA
                     AWVVVQVLIFTIQPGETSYMLFLAGLAGIGVSAAYILPDSILPDVIEWDELRTGRRQE
                     GIYYGIRTLIRKLTGALIIFITLQILGWSGYQAPPENVTVYQQPESALFMIRLMDSFI
                     GAAILAGTIILAWSYPLTREKYQKIQKLLAVRRNKNAETT"
     gene            complement(641..>1980)
                     /locus_tag="PLR93_11270"
     CDS             complement(641..>1980)
                     /locus_tag="PLR93_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH27595.1"
                     /translation="IAFDLATLMGYDTDQPEALGEFGKCGVAVSSLKDMEILLDGIPL
                     DKVSSSMTINSPASIIWAMYLVAAEKQGVRLDQLRGTLQNDILKEFIAQKEFIFPPEP
                     SMRLVVDTMEYGSQHVPQWNTISISGYHIREAGSTAAQELAFTLADGMEYVRWGIARG
                     MDVDEFAPRLSFFFNAHNDFFEEIAKYRAARRIWAREMRETFKAKNPRSWLMRFHTQT
                     AGVSLTAQQPENNIVRVAIQALAAVLGGTQSLHTNSMDEALALPSEHAVKVALRTQQI
                     IAEESGVTNTVDPLGGSFFVEAQTDRMEKDAYAYFQRIQDLGGVIPALEKGFMQSEIS
                     DAAYRYQREIDENRRKIVGVNAYLDEKPAAIPILKMDPNGYSRQMNRLNEVRKTRDSG
                     RVGQTLDALRIACEGTENTMPYILDCVRAYCTLGEIIGVMTKVFGKYEEPTMI"
BASE COUNT          448 a          492 c          515 g          525 t
ORIGIN      
        1 tccttggtgt agaccttgct ttagagtctg ccttctttgg ggtgttgatg ttggtttgtg
       61 tgttgttcgt gccgttctgg ctgtggctat cgaaacgaca aaataagatc agagcgtaca
      121 tccttggtat ggcggcgtgg gttgttgtgc aggtgttgat cttcactatt caaccgggcg
      181 aaaccagtta catgttgttc cttgccgggt tggccggtat tggcgtttct gccgcttaca
      241 ttttgccaga ctcgattttg ccagatgtga ttgaatggga tgaacttcgt acggggcgca
      301 gacaagaagg tatttattac gggatccgta cgctcatccg taagttgacc ggggcgttga
      361 tcattttcat cacgctgcaa attttgggtt ggtctggcta tcaggcgccg ccagaaaatg
      421 tgaccgtgta ccaacaaccg gaatctgcct tgttcatgat tcgcctaatg gactcgttca
      481 tcggcgcagc aattttggcg ggcacgatca ttctggcatg gtcttatccg ctcacacgtg
      541 aaaaatatca gaagattcaa aagttactgg ctgttcgccg aaacaaaaac gcggaaacga
      601 cttgatcgtt tccgcgtttt gctataaaag gcgagagcgc ctagatcatc gttggttctt
      661 cgtactttcc aaaaaccttc gtcatcacac cgatgatttc ccctaatgtg caataggcac
      721 gcacacaatc caaaatataa ggcatggtgt tctcggttcc ttcgcaagcg atgcgcaaag
      781 cgtcgagagt ttgccctacg cgaccactat cgcgcgtttt gcggacttcg ttcaggcgat
      841 tcatctgacg gctgtatccg ttcgggtcca ttttgaggat tggaatggcg gcaggttttt
      901 catcgagata cgcattgacg cccacgatct tgcggcggtt ctcatcgatc tcacgttgat
      961 aacgatacgc cgcatcggag atttcgcttt gcatgaaacc tttttctaac gcggggatga
     1021 ctccacctag atcttgaatg cgctggaagt aggcgtacgc atctttctcc attcggtctg
     1081 tttgcgcttc gacgaagaaa ctacccccaa gcgggtcgac cgtattcgtc acgccggatt
     1141 cttctgcgat gatctgctgc gtccgcagag caactttcac agcatgttca gagggcagtg
     1201 caagcgcttc atccatcgag ttggtgtgca aactttgcgt tccacccaat accgcagcca
     1261 atgcctgaat cgccacgcgg acgatgttat tttccggctg ttgtgccgtg agcgagacac
     1321 ccgctgtttg agtgtggaag cgcatcagcc acgagcgcgg attcttcgct ttgaaggtct
     1381 cgcgcatttc tcgcgcccaa atacggcgag ccgcacgata cttggcaatc tcttcaaaga
     1441 aatcgttatg ggcattgaag aagaaagaga gacgcggcgc aaattcatct acgtccatgc
     1501 cgcgtgcaat gccccagcgg acatattcca tgccgtctgc aagggtgaac gccagctctt
     1561 gcgcagccgt cgaacccgct tcacggatgt gatagcccga aatggaaata gtattccact
     1621 gcggcacgtg ttgtgagccg tattccatcg tatccactac gagacgcatg gaaggttctg
     1681 gtgggaagat gaattccttc tgtgcgataa attctttgag aatgtcgttc tgaagggtgc
     1741 cgcgtaattg atcgagacgc acaccttgtt tttcagcggc tacaagatac attgcccaga
     1801 tgatggaagc cggcgagtta atagtcatgc tcgaagaaac tttatcgagc ggaatgccat
     1861 ccaaaagaat ttccatatct ttaagcgaag aaacggctac accacatttg ccaaattcgc
     1921 ccagcgcttc gggttggtca gtatcgtagc ccatcagcgt ggcaaggtcg aaggcaatgc
//
LOCUS       DAOQFT010000497         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39535_length_1977_cov_4.099376, whole genome shotgun sequence.
ACCESSION   DAOQFT010000497 DAOQFT010000000
VERSION     DAOQFT010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39535_length_1977_cov_4.099376"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLR93_11275"
     CDS             complement(<1..985)
                     /locus_tag="PLR93_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27596.1"
                     /translation="MSEELESTQASRRSDLGGTQPSQPYKEDDTQPLKVKKPSRWRSI
                     LLGVLGFLLLISVGGFGGYSAGIGVRKNAEQSILATQLSEQFSFALVDIQFGRYENAR
                     QRLEYIIQRDPNFPGAQEKLTEVLVLSSVPTPTAAPTATATPDFSGAESAFARAQELT
                     RAQDWNGALTALDTVRKLDPSFKTAQVDGMYYFALRNRGYDLITKEGNLEGGIYFITL
                     AERFGPLDNAAKGVRDGARAYMTGASFWDLDWEQAAQYFETVAAGWPSMWDGTMTAGQ
                     RYYTAMLRLADEAFTAGEYCGAYEQYQKAASYGVLDGEASANSAQAYAACYP"
     gene            complement(1038..>1977)
                     /locus_tag="PLR93_11280"
     CDS             complement(1038..>1977)
                     /locus_tag="PLR93_11280"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNH27597.1"
                     /translation="IHGLVYGVIIGAAFHLLIQIPGLFMYGFRWTPALDIRHTGLIEA
                     IKLMLPRLFTMAGIQIIFLARDNLASRLDQVGAVTSLTYGWMIMQVPETVLGTAIATA
                     MLPALAELASKQDWHGFSATVERALRVLIALTLPIAAVMAAGIHPLVRGVFGFDEQIS
                     TLITWTTRAYLATLTGYTIHEIAIRAFYARKEPMVPLYAIGIRLGIFLVIGILGVTFF
                     QNIGAPVIAFAEIALLIEAIFILGWLSKRTHEPLHTSDVVFRGLIAAVAGGIVTYLVA
                     LYLPGGAIVTALIGMIVGGVVALAIVWNEAKQLLKL"
BASE COUNT          491 a          458 c          557 g          471 t
ORIGIN      
        1 cggggtaaca tgccgcatac gcctgtgctg agtttgctga ggcttctcca tctaagacac
       61 catacgatgc tgctttttga tattgttcgt acgcgccaca atattcacca gcggtaaatg
      121 cttcatctgc taagcgcaac atggctgtat agtaacgttg acctgccgtc atggtgccat
      181 cccacatgga gggccagccc gcagcaactg tttcaaaata ttgtgcggct tgttcccaat
      241 caaggtccca gaagctggcg ccggtcatgt aggcacgagc tccatcgcgg acacctttgg
      301 ctgcattatc aagcggacca aaacgttcgg caagcgtgat gaagtagata ccaccttcaa
      361 ggttgccttc ttttgtgatg aggtcatatc cgcgattgcg cagtgcaaaa taatacatgc
      421 catcgacttg ggcagttttg aaggaagggt ctaatttacg aactgtgtct aatgcggtga
      481 gtgctccgtt ccaatcttgg gcacgagtga gttcctgtgc tcgggcaaaa gcgctttctg
      541 caccgctgaa atcaggggtg gcagtagcag tgggggcggc ggtaggagtg ggaacactgc
      601 taagaaccag tacttcggtt aatttttctt gcgcaccggg gaaattcggg tcacgttgga
      661 tgatgtattc caagcgttga cgggcatttt catagcgacc aaattgaata tctacgagag
      721 caaaagagaa ttgctctgaa agctgggtag caaggatgga ttgttctgca tttttgcgaa
      781 caccgatccc cgcactatac ccgccgaagc cgcctacact aataagtagt aagaagccta
      841 aaacaccaag aaggattgaa cgccaacgtg aaggtttttt gaccttgagc ggttgagtat
      901 catcttcttt atatggttgt gagggttgcg ttccgccgag gtcggagcga cgactagctt
      961 gagtggattc gagttcttca gacatgtgcg tgattataca ttcttttgat gggtcagttt
     1021 tttttcttaa ttgacattta taacttcaac aattgcttgg cttcgttcca aacaattgct
     1081 aatgcaacca ctccaccgac aatcatgccg atcaatgcgg tgacaattgc gccgccgggg
     1141 aggtagaggg cgacgaggta ggtcacaatt ccgcctgcaa ctgcggcgat gagccctctg
     1201 aagaccacgt cactggtgtg gagcggttcg tgagtccgtt tggatagcca gcctaagatg
     1261 aatatcgctt caattaatag cgcaatttcg gcaaaggcaa tgaccggagc gccgatgttt
     1321 tgaaagaaag tcacccctag aatgcctatg acgaggaaaa taccgagccg aatgccaata
     1381 gcgtataaag gaaccatggg ttctttgcga gcatagaagg cgcggatggc aatttcgtgg
     1441 atggtatacc cggtcaatgt ggcgaggtag gcacgggttg tccacgtgat gagggtggag
     1501 atttgttcgt cgaagccgaa gacgccgcgc acaagcggat ggatacctgc tgccatgacc
     1561 gccgcaatgg gcaaggttaa ggcgataagg actcggaggg cacgttcgac tgtagcgctg
     1621 aacccgtgcc agtcttgttt tgaggcgagt tctgccaggg cggggagcat ggcagtggcg
     1681 atggcagtcc ccagcacggt ctctggcact tgcataatca tccaaccata ggtgagggag
     1741 gtgactgcgc cgacttggtc gaggcgtgag gcaaggttat ctcgtgcaag aaaaatgatc
     1801 tggatacctg ccatcgtgaa tagacgcggc aacatcagtt tgatcgcttc gattaaaccg
     1861 gtgtgacgga tatccagtgc gggcgtccag cggaagccgt acatgaatag accagggatt
     1921 tgaataagca gatggaacgc cgccccaata atgacgccat acaccaagcc gtgaatg
//
LOCUS       DAOQFT010000498         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39801_length_1970_cov_7.537337, whole genome shotgun sequence.
ACCESSION   DAOQFT010000498 DAOQFT010000000
VERSION     DAOQFT010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39801_length_1970_cov_7.537337"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PLR93_11285"
     CDS             <1..1416
                     /locus_tag="PLR93_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH27598.1"
                     /translation="AQLANGRSSFVFFIPVAVASLLLTPASSFIFAIAGVAIMIWLAN
                     EGNVLPNVSTISGEFMFALVSWLSARSLEQALVDLRATNQNLDQLVEQKTRELANTLS
                     RELIVAGRNQAILNSIADGVIVFDERNMAILANPAISRLTNIPVSELVDRSMVDFILR
                     DALSESRDSLQQIFEDPEEETITGVRVNWGSKTLSASVARVNDADDKVIGTVAVFRDI
                     TREVELEKMKDTFMGIVSHELRTPLNAILGYAEMLKEGIYGAVNEKQANIAERVMNST
                     QRLLSIVSDLLDQAQIQSGKLKIRMMSCNPVEMLESLHGVMDRIALEKGIEFRTDIDP
                     AMPTVIMGDPQRLQQVMINLANNAIKFTEKGYVSVKILRMDADHWQIRTTDTGSGIPT
                     DAQEYIFETFRQVESSAIRQHGGVGLGLSIVKQLVELMKGKITLESEIGKGSTFTVTF
                     PIVINDDSQPTVTDPDRRNYE"
     gene            1409..1777
                     /locus_tag="PLR93_11290"
     CDS             1409..1777
                     /locus_tag="PLR93_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH27599.1"
                     /translation="MNDPLALIIEDDEDLAEIFGQALTAAGYATEIVRDGLAAQTRIK
                     DVTPAIITLDMHIPQISGDRILQQIRSDQRLLKTRVVVTTADAIMAENARGIADLVLI
                     KPITFSQLRDLSARLRPRNT"
BASE COUNT          503 a          451 c          491 g          525 t
ORIGIN      
        1 gctcagcttg caaatggacg ttcatcattc gtgttcttta tccctgtagc ggttgccagt
       61 ttgctcctca cccctgccag cagttttata ttcgcaattg caggcgtggc tattatgatt
      121 tggttggcga atgaaggcaa tgttttgcca aatgtgtcaa caatttctgg cgagtttatg
      181 tttgcgctgg tttcatggct ttcggcacgt tcactggaac aagctctggt tgacctgcgt
      241 gccaccaatc aaaaccttga tcagttggta gagcaaaaga ctcgcgaact tgccaatact
      301 ctttctcgtg aattgatcgt ggctggacgt aaccaggcga tcctcaactc gattgcagat
      361 ggcgtgatcg ttttcgatga acggaatatg gcgatccttg ccaaccctgc catcagccgt
      421 ctcacaaata tacccgtttc agaacttgtc gaccgcagta tggtggattt tattttgcgc
      481 gatgcccttt ctgaatcgcg tgacagcttg caacagatat ttgaagaccc cgaagaagag
      541 accatcaccg gtgttcgtgt caactggggc agtaagaccc tctctgccag tgtggcgcgt
      601 gtgaacgatg ccgatgataa agtgatcggt actgtggcag ttttccgtga tattactcgt
      661 gaggtagagc ttgaaaagat gaaagatacc tttatgggca tcgtatctca tgaattgcgc
      721 accccgttga acgctattct tggatacgct gaaatgttga aagagggtat ctacggtgcg
      781 gtcaacgaga aacaagctaa tatcgctgag cgtgtgatga acagcaccca acgtttgctg
      841 agcatcgtca gcgatttgtt ggatcaagcg cagattcaat caggtaagtt gaagatccgc
      901 atgatgtcct gcaaccctgt tgagatgtta gagtccttgc atggtgtgat ggaccgtatc
      961 gctttggaaa aggggattga attccgcacg gatattgacc ccgccatgcc aacggtcatt
     1021 atgggagacc cacaacggtt gcagcaggtg atgattaacc ttgcaaacaa tgccatcaaa
     1081 tttacagaaa aaggatatgt cagtgtcaag atcctgcgca tggatgcaga tcattggcag
     1141 atccgtacca ccgatacagg cagtggcatc cctactgatg cacaggaata tatttttgag
     1201 accttccgcc aggtagaaag cagtgctatt cgtcaacatg gcggggttgg gcttgggctt
     1261 tccatcgtca aacagcttgt cgaattgatg aaaggaaaga tcaccttgga gagtgaaatt
     1321 gggaagggca gtacgtttac ggtcactttc ccgatcgtaa ttaatgacga ttcgcaaccc
     1381 accgttacag atccagatag gagaaactat gaatgaccct ctcgcgttga ttattgaaga
     1441 tgatgaagac ctcgccgaga tctttgggca ggcacttact gctgccggat atgccacgga
     1501 gattgtccgt gatgggcttg ctgctcaaac ccgcatcaaa gatgtaacgc ccgctattat
     1561 cactctggat atgcatatcc cgcaaatatc cggtgaccgc atccttcaac aaattcggag
     1621 cgaccaacgt ttgctaaaga caagggtggt cgtcacgact gcagatgcga tcatggcaga
     1681 aaacgcccgt ggcattgcag accttgtctt gatcaaaccg atcacatttt cccaattgcg
     1741 agatctaagc gctcggttgc gccctcgtaa tacatagagc ttctccttat agtgtaagtc
     1801 ccggcactcg gttagagaga agttattttg tacgggtgtc gggtctgtat tggatgaaga
     1861 taacaacttg cgttacctat aaccctcctg tgctacactt atggcatgga gatttgttac
     1921 ttaatagctt ttcctgatac agaacataaa gttgcaccgg taaaagagtc
//
LOCUS       DAOQFT010000499         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39977_length_1965_cov_4.940314, whole genome shotgun sequence.
ACCESSION   DAOQFT010000499 DAOQFT010000000
VERSION     DAOQFT010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39977_length_1965_cov_4.940314"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLR93_11295"
     CDS             <1..309
                     /locus_tag="PLR93_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNH27600.1"
                     /translation="LAPERCRVALSLKRMLPNPWTNAINEFPIGSVHNATITSVLSYG
                     VFARLDKYGVEGLVHASEIPHEEGTLLKDFLSDGQAIQVRVLHLDPAHHRLGFSMRLE
                     "
     gene            313..>1965
                     /locus_tag="PLR93_11300"
     CDS             313..>1965
                     /locus_tag="PLR93_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNH27601.1"
                     /translation="MSKPTETRRYAQEDFSPRTDWLGRLARLNTQFGRFTRDLIGVSL
                     ISFSVMAFFAIWNWTDGFVLTPIALILRTWFGWGSLLILAGIAYLGYSFLQRQQTSLH
                     WGRILALELGALLTLGLLAIPFGNDLFRAESGMDGGRLGWGIVRLLWQMGTIPGTLVL
                     LLLWALSVATGFGLWAKLEAWLIKIAGDEAPVIVSMPEPEVPQEIAVEQPVSKEAAAP
                     KKKAPAIPPEFRTQLKSAEKKPEKSLKPPPRGEDLPPLTVLMGDSTVRTDERTINQTA
                     GLIMKTLSEFGIPATVIGYRVGPSVTQFAVQPGFIKKGNEEEDAQQMKVRVAQIASLE
                     KDLALALSAQRLRIEAPVPGKPYVGIEVPNINSSIVRMRALLESDAFYKVGAPLTIAL
                     GRDVSGNPLMADLSRMPHLLIAGSTGSGKSVCVTAIAACLAMNNTPEELRIVMIDAKM
                     VELIRFNGLPHLYGKVETNLERILGVLRWVVVEMEHRYRLLESMHARDLNSYNKKVSR
                     SADGEQLPRIVVIIDELADLMMSAPDQTEHNLVRLAQMARATG"
BASE COUNT          444 a          478 c          515 g          528 t
ORIGIN      
        1 cttgcgccag aacgctgccg tgttgcctta agtttgaagc gtatgcttcc caacccttgg
       61 acgaatgcga taaatgaatt cccgattggt tctgtgcata atgcaaccat caccagcgtt
      121 ttatcttacg gcgtgtttgc acggctggat aaatatggtg tggaaggctt ggttcatgct
      181 tctgaaattc cgcatgaaga aggcacattg ctgaaagact ttctctctga cgggcaagcg
      241 attcaggtgc gtgtattgca tctcgaccct gcacatcacc gccttggctt cagcatgagg
      301 cttgaataag ggatgtctaa acccactgaa acaaggcgtt acgctcaaga agatttttct
      361 ccgcgcaccg attggctcgg aaggcttgcg cgtcttaaca cgcaatttgg gcgtttcact
      421 cgcgatctta ttggtgtaag cctaatctcg ttttcagtca tggcattctt tgccatttgg
      481 aactggaccg atggctttgt gcttacacca atcgccttga tattgcgtac ttggtttggg
      541 tggggtagtc tcttgatttt ggcagggatt gcttatcttg gatattcctt tttacaacgc
      601 caacagacgt ctctacactg ggggcggatt cttgctcttg aactcggcgc tctcttaacc
      661 ctgggcttgc ttgctatacc ctttggcaac gacctcttcc gagccgaatc tggcatggac
      721 ggtggaaggt tggggtgggg tattgtgcgc ctgctgtggc aaatgggaac catccccggc
      781 acgttggtgc ttttgctttt gtgggcgttg agtgttgcta ctgggtttgg gctgtgggca
      841 aagcttgaag catggctcat aaaaattgca ggtgacgaag cgcctgtgat cgtctctatg
      901 ccagagccag aagttccaca agagatcgct gtcgaacagc cagtttcaaa agaagctgct
      961 gctccgaaaa agaaagctcc tgccattccg cctgagtttc gtactcagtt gaaatctgct
     1021 gaaaagaagc cagagaaatc actcaagccg cctccacgcg gtgaagattt accgcctctc
     1081 actgtcttga tgggcgactc aactgtccgc accgatgaac gcacgatcaa ccaaactgcc
     1141 ggtttgatca tgaaaactct ttcagagttc ggtattcccg caacggtaat cgggtatcga
     1201 gttggtccat ctgtgacgca gttcgctgtt cagccgggct tcatcaaaaa aggtaatgaa
     1261 gaagaagatg cccaacagat gaaggttcgt gtggcgcaaa tcgcttcact ggagaaagac
     1321 cttgcattgg cgctatctgc tcaacgtcta cgtattgagg cgccggttcc gggcaaacct
     1381 tatgtgggta tcgaagtccc gaacattaac agttctatcg tccgcatgcg agccttgttg
     1441 gaaagcgatg ctttctataa agttggcgct ccgttgacca ttgccctggg acgtgatgtc
     1501 tctggcaacc cgttgatggc agatttatct cgtatgccgc acttattgat cgcaggttct
     1561 acgggatctg gtaaatcggt gtgtgttact gcaattgcgg cttgtcttgc catgaacaac
     1621 acgcccgaag aactgcgcat tgtgatgatc gatgccaaga tggtggaatt aatccgtttc
     1681 aatggcttgc cacatctgta cggaaaagtg gagaccaacc ttgaacgcat tcttggtgtg
     1741 ttgcgttggg tggtcgttga aatggagcat cgctatcgct tgctcgaatc gatgcacgcc
     1801 cgtgacctga attcgtacaa caagaaggtc tcacgttctg ccgatgggga acaactcccg
     1861 cgcattgtag tcatcatcga tgaactcgca gacttgatga tgtctgcgcc agaccagacc
     1921 gaacataacc tcgtccgctt ggcacaaatg gcacgtgcta cagga
//
LOCUS       DAOQFT010000500         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_39996_length_1964_cov_6.255631, whole genome shotgun sequence.
ACCESSION   DAOQFT010000500 DAOQFT010000000
VERSION     DAOQFT010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39996_length_1964_cov_6.255631"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..390)
                     /gene="ssrS"
                     /locus_tag="PLR93_11305"
     ncRNA           complement(214..390)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLR93_11305"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            519..1544
                     /locus_tag="PLR93_11310"
     CDS             519..1544
                     /locus_tag="PLR93_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNH27602.1"
                     /translation="MNLVHGVDVSYWEPKIDWQALRSQGFKFALIRATSGVGYVDPKF
                     KSHWEGARKEGLLRGAYHYLIANQDPKQQADLFISNISADRGELPPIIDLEDKYNESA
                     KNTQIIDTCKAILDRVEKAFGVKPMIYSRTQYLNLHVSRNGAAPSWAKDYPLWLAQYP
                     YVFDANTMPNKNMPTQATGWQDWLFWQYSETTLVDGVTSDNGQPTECDLNWFRGTEEE
                     LYAFANVKPKEPVSYTVKSGDTFKAIAEKYKITVDELLAANPQLLADGTKLSIPVPTI
                     SKPIVSDNSTTNKATSSTRTHTVKQGDTIGTIAQKYGVTVDAIKALNPQLTNPNLIYV
                     GQVIVIP"
     gene            complement(1617..>1964)
                     /locus_tag="PLR93_11315"
     CDS             complement(1617..>1964)
                     /locus_tag="PLR93_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNH27603.1"
                     /translation="VFVYGYPSAVKAFYMEPWENRPEVCKSVDLLAPEGYGEICGGSE
                     RMSDPDKLLARIQHEGLPEEAFKWYLELRKYGSVPHAGFGMGTERVTAWMCGIEHIRE
                     AIPFPRTIKRIYP"
BASE COUNT          498 a          470 c          501 g          495 t
ORIGIN      
        1 agcttggtag ttagcatccc gttcgcacgg gagaacctcc acctcgtgat ctcgtttaca
       61 acgagaccca gccgcttgta tctccgttat ttagttgtgc tcacatccct aacaacgggc
      121 gggattttac acgcgggttg caagtttcgt caagcaagtg atctacacaa taaaagcgga
      181 atgttccact caggaacatt ccgcttcaca attgcgtacc ccgcctgtgc cgtgtaccgc
      241 ggggttttga acctgctatt gcaggtgggt tcctaccaag caacgccgcc ttggctcagg
      301 cctatcgcgt tgttactgta aagttaagga cccttttagt gagtattggc tcaaaacgga
      361 agggcgatat cacgagcacc gcagggtgcg attcttatac catactctgc gagacaagga
      421 aagatactct ctaagtatgc ttttaacgaa gagattgatt ttctcaaaaa attgtgctag
      481 gctaatttga gcagtgtttt tattcaaagg agagaacaat gaatttagtc catggtgttg
      541 acgtaagtta ttgggagcct aaaatcgatt ggcaggctct gcgttctcag gggtttaagt
      601 ttgctttgat tcgtgccact tctggggtgg ggtatgtgga ccccaagttt aagtctcatt
      661 gggaaggagc acgcaaagaa ggtctcttgc gtggggcgta tcattacctg atcgccaacc
      721 aagacccgaa acaacaagca gaccttttca tttccaacat cagtgcagac cgtggggaac
      781 tgccgcctat tattgacctt gaagataaat acaatgagag tgctaaaaac acgcagatca
      841 ttgatacatg caaagcgatt ctagaccggg tggagaaggc gttcggtgta aaaccgatga
      901 tttattcacg cacccagtat ttgaatttgc atgtctcacg gaatggagcg gcgccttcgt
      961 gggcgaagga ttatccgctt tggttggcgc aatatccgta tgtgtttgat gccaatacca
     1021 tgccaaataa aaacatgcct actcaggcga caggttggca agattggctc ttctggcaat
     1081 attctgaaac tacgctagta gatggtgtga cgagtgataa tggtcagcct accgaatgtg
     1141 acctgaactg gttccgcgga acagaagagg aactctacgc atttgcgaat gtcaagccga
     1201 aagaaccggt cagctacaca gtgaaaagtg gcgacacctt caaagcgatc gccgagaaat
     1261 ataagatcac ggtggatgaa ttgctggcgg caaatccgca gttactggca gatggcacga
     1321 aactttccat ccctgtgccg acaatttcga agccaatcgt cagcgataat tccacgacga
     1381 ataaggcaac ttcctccacg cggacacata ccgtaaagca gggcgatacg attggtacca
     1441 tcgctcaaaa atacggcgtg accgtggatg ccatcaaagc tctcaacccg caattgacca
     1501 atcccaattt gatctatgtg ggtcaggtga tcgttattcc gtgattggat taaatataaa
     1561 catccccgtc gcttctttcg acggggatgt ttgattctgt ttcctgaaag tttttcttac
     1621 gggtaaattc tcttgatggt tcttgggaag ggaatcgcct cgcggatgtg ttcgatgccg
     1681 cacatccagg cggtgacacg ttcggtgccc atgccgaagc ctgcatgggg gacagagccg
     1741 tacttgcgca gctccagata ccacttgaag gcttcttcgg gcagaccttc gtgttggatg
     1801 cgtgccagaa gtttatccgg gtcgctcatg cgttcggagc cgccgcagat ttcgccgtag
     1861 ccttcgggcg caagcaaatc cacgctcttg cagacttcgg ggcggttttc ccacggttcc
     1921 atgtaaaagg ctttgaccgc gctcgggtag ccatacacaa acac
//
LOCUS       DAOQFT010000501         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_40105_length_1961_cov_6.636411, whole genome shotgun sequence.
ACCESSION   DAOQFT010000501 DAOQFT010000000
VERSION     DAOQFT010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40105_length_1961_cov_6.636411"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLR93_11320"
     CDS             complement(<1..592)
                     /locus_tag="PLR93_11320"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNH27604.1"
                     /translation="MNETIFRILAAVILFTGVGISIYFRSKADRTSGEKLSRNVDGTP
                     LMLVIRIFGLVLWLSPLVYLVNPVWMNWSKISLPAWSRWFGVVFGIFCVFGIYWLFTS
                     IGSGITPVSATRKEHKLATSGIYRWIRHPLYTVGSSFIVSFGLIADNWFIIALGMLAF
                     TIMAIRTPKEEENLIEKFGDEYRDYMQRTGKFFPKFK"
     gene            complement(653..1315)
                     /locus_tag="PLR93_11325"
     CDS             complement(653..1315)
                     /locus_tag="PLR93_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNH27605.1"
                     /translation="MKTSTSIQDYLKRIYELTESGQSASTNDLARELKVKPASVTGMI
                     QKLAAETPALVEYQKHQGVTLTVAGKRAALEVIRHHRLLETWLVQTLGYSWDEVHEEA
                     ERLEHVISEDFERRIAAALGHPLRDPHGELIPTEDLKMPLDESTPLSALRKDQSGTIL
                     CIKAADADLLRHLTELGLTPNTFIQVTEYSPYDHNLTIRVDKKTHVLGLSITSKIFIE
                     EG"
     gene            complement(1319..>1961)
                     /locus_tag="PLR93_11330"
     CDS             complement(1319..>1961)
                     /locus_tag="PLR93_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27606.1"
                     /translation="ATTYAVGDVLLLASKISLDDYPKLKPFAKLLSDSEKMVALSPIR
                     MMWGALLGVFATPLVLFGFWHAYQGLSGVNAWAALIAFFLFALASIIGTFVHGTFYYM
                     GEYVHALNKVSTDSQAVIVEMIERHKKVLIITYAPLLVFIIFASILFSILVASGNTLF
                     PVWMTAINPLTLTILWMLGKRILPQFVRDWTEGAGFNIAYMAFFVCTTITLWK"
BASE COUNT          525 a          441 c          476 g          519 t
ORIGIN      
        1 atttgaattt cgggaagaat tttcccgtcc gttgcatata gtcgcgatat tcatcgccga
       61 atttctcaat gagattttct tcttctttcg gcgtacggat tgccatgatc gtgaatgcca
      121 acatacctaa tgcgatgatg aaccagttat ctgctatgag accaaacgag acgataaacg
      181 atgaacccac ggtataaagt ggatgcctta tccagcggta gatgccactg gttgcaagtt
      241 tatgttcttt acgcgttgca ctgactggtg taattccgct gccaatactg gtgaacaacc
      301 aataaattcc gaagacacaa aatataccga acacgactcc gaaccaacgg ctccatgcgg
      361 gtaaactgat cttcgaccag ttcatccata cagggttgac taagtagacg agcggactga
      421 gccagagcac caacccaaag atgcgaatga ccagcataag cggagtgccg tctacattgc
      481 gagatagttt ttctcctgaa gtacggtctg ccttgctacg gaaatatatg gagatgccta
      541 cacctgtaaa aaggataacg gctgccaaga ttctaaaaat ggtttcgttc atagtgttaa
      601 agcctttctt ctttctgttt cttacttttc atttattcga taactttgtc gattaacctt
      661 cctcaatgaa tatcttgcta gtgatactca accccaacac atgtgtcttt ttatcaaccc
      721 gaatggtaag gttgtggtcg tacggagaat attctgtcac ctgaatgaac gtgtttggtg
      781 taagccccag ctcggtcagg tgccgaagca ggtctgcgtc tgcggctttg atgcataaga
      841 tcgtgccaga ttggtctttt cgcaaagcag aaagcggagt ggactcatcc agcggcatct
      901 taagatcttc ggtggggatc aactctccgt gcgggtcacg aagcggatgc cccaaagcgg
      961 cagcgatgcg tcgctcgaaa tcctctgaaa tgacatgctc caatcgttct gcctcttcat
     1021 ggacttcatc ccatgaatac ccaagggttt gtacgagcca ggtttccagc aaacgatgat
     1081 ggcggatcac ttccagagcg gcacgtttgc cagccacggt gagagtgacg ccctgatgct
     1141 tttggtattc gaccaaggca ggcgtttctg ctgcaagttt ttggatcata ccggtgacag
     1201 atgctggctt gacttttagc tcacgcgcca gatcattggt gctggctgat tgcccgcttt
     1261 cggtcagttc gtagatgcgt ttgagataat cttgtatgga tgtggatgtt ttcattgatt
     1321 atttccaaag agtgatagtc gtgcagacaa agaatgccat atatgcgatg ttgaaaccgg
     1381 caccctccgt ccaatcgcgg acgaattgcg gcagaatgcg ctttccgagc atccaaagaa
     1441 tcgtgagggt aagtgggttg atcgctgtca tccatactgg gaataaagta ttcccagaag
     1501 cgaccaaaat cgaaaatagg atagaagcga aaataatgaa caccaacaac ggggcatacg
     1561 tgatgataag aacttttttg tgtctttcaa tcatttctac gattactgct tgcgagtcag
     1621 tggagacctt gttcaaggca tgtacatact cgcccatgta gtaaaacgtt ccatgtacaa
     1681 aggttccgat gatggatgcc agtgcaaaaa ggaaaaaagc aattaatgct gcccaggcgt
     1741 tgactccgct taagccttga tatgcatgcc aaaacccaaa tagtacgagg ggagttgcaa
     1801 acactccaag taatgcgccc cacatcatgc ggatggggga gagtgccacc attttttctg
     1861 agtcagaaag tagtttggca aatggcttga gttttgggta gtcgtcgagg gagatttttg
     1921 aggcaagtaa aagcacatca ccaacggcat aggtggtcgc a
//
LOCUS       DAOQFT010000502         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_40359_length_1954_cov_5.385993, whole genome shotgun sequence.
ACCESSION   DAOQFT010000502 DAOQFT010000000
VERSION     DAOQFT010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40359_length_1954_cov_5.385993"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..695
                     /locus_tag="PLR93_11335"
     CDS             159..695
                     /locus_tag="PLR93_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27607.1"
                     /translation="MLIGSLVIFAGAYWAHMSVGLFDLRFFQYYFLFLAGVYFCRFES
                     IRIHLFSWNGLIKVFLAFLSIWLFALVLNAGLEMIHGLYILAVDFFILSWILLTLSIF
                     RTKFGDWPGWVWISTASFFAYLFHRPLWYAMDGFFGLEPGPTRVWVHLVPGAILALIL
                     GYVLQIGYDRLLAVLRLK"
     gene            751..1887
                     /locus_tag="PLR93_11340"
     CDS             751..1887
                     /locus_tag="PLR93_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNH27608.1"
                     /translation="MSDALPPPVWVNTTESLSRMVNDIASQSRVAVDTESNSLHAFRE
                     QVCLLQFSTTRADYLVDPLILEDLDLLAPIFSDPKIEKVFHAAEYDLTCLRRDFHFDF
                     TNLFDTMHAARVLGYPAVGLDKLLGDKFGITMDKRHQKADWAARPLTKEQIHYARLDT
                     HYLFDLRDALEMELLEKERLQFALEDFQRACRVDDVRHRSNGESWERFSGRKDLSLRE
                     LTIIAHLCKWRDKEAERLDRPSYKVVMDEVLITLAKQPPEAKVDLSGAGLSEKQIRLW
                     GDMILKAVKVGADAPLVERKQAERKQDAVLRRLEKLKVWRKKAAEDLKVESDIVLPKV
                     YLGVLSETPPKDMADLGHLMKDAPARFEKYGLQILKTLGVKHAN"
BASE COUNT          452 a          376 c          532 g          594 t
ORIGIN      
        1 taaacagggc cgggttggct gtgtatgcct tgaatgtgca ggcggtattt tcccccacct
       61 ttgttaaatc tttgctgacg ctttggtata taagtatgtt ggtggtgttt tacatcctgt
      121 atggggtctt gatggtgaat atccgttcga actggggttt gttgatcggt tcgcttgtga
      181 tctttgcagg cgcttattgg gcacatatgt cagtgggatt gtttgacctg cgtttctttc
      241 aatattattt tctcttttta gcaggtgtgt atttttgtcg gtttgaatcg attcgcatac
      301 atttgttttc ctggaatggt ctgatcaagg tttttcttgc atttctcagc atttggcttt
      361 ttgctcttgt cttgaatgcg gggttggaaa tgatacacgg cctttatatt ttggctgtcg
      421 acttctttat ccttagctgg attttattaa ccctttccat ttttaggaca aaatttggag
      481 attggcctgg atgggtttgg atatccacgg catctttttt tgcatatctg ttccatcgtc
      541 cgctatggta tgcaatggat ggtttctttg gcctggagcc ggggccgaca agggtgtggg
      601 ttcatttggt tcccggtgcc attcttgcgc ttattttggg atatgttctt caaattgggt
      661 atgaccgtct gctggccgtt ctacgcctga aatgaagaag taagattgga aaggttgtta
      721 ctataatctt cgggtttata atcgaacaat atgtctgatg cactcccgcc acccgtatgg
      781 gtcaacacaa ccgaatcgtt aagcaggatg gtgaacgaca tcgcctcaca atctcgtgtg
      841 gcagtcgata ctgaatcaaa cagcctgcat gcatttcgag aacaggtatg tctgcttcaa
      901 ttttcaacga ccagggcgga ttatctggtg gacccgctta tattggaaga tcttgatctt
      961 ttggcgccta tattttctga cccgaagatc gagaaggttt ttcatgccgc cgagtatgac
     1021 ctgacctgtc tgcggcgtga ttttcatttc gattttacaa acctgtttga tacgatgcat
     1081 gcggcgcgtg tgttggggta cccggcagtt ggcttggata aattattggg cgataagttc
     1141 ggaataacaa tggataaacg gcatcaaaag gcggattggg ctgcccgtcc attaacaaag
     1201 gaacagatcc attacgcgcg gctggatacc cactatttat ttgatctgag agatgctttg
     1261 gagatggaat tacttgagaa ggagagattg caatttgccc tggaagactt ccaacgtgcg
     1321 tgccgggtgg atgatgttcg gcatcggagc aatggcgagt cttgggagcg tttctctggt
     1381 cgtaaggact tgagcctgcg agagttgacc ataatagctc atctatgcaa atggcgtgac
     1441 aaggaagctg aacgtttgga ccgaccctcc tataaagtgg taatggatga agttctgatc
     1501 accctggcga aacaaccccc tgaggcaaaa gttgacctct cgggtgcggg gctttctgaa
     1561 aagcaaattc ggttatgggg agatatgatc ttaaaagcgg taaaggttgg ggcggatgct
     1621 ccgcttgtgg agcgcaaaca ggctgaacga aagcaggatg cggtgctgcg tcgactggaa
     1681 aagctgaaag tttggagaaa aaaggcggcg gaagatctca aggttgagtc ggatattgtt
     1741 ctgccgaaag tgtatttggg tgtcctttcg gagacgcctc cgaaggatat ggctgacctg
     1801 gggcatttga tgaaggatgc cccagcacgc tttgagaaat atggattaca aatcctgaaa
     1861 actttaggag taaaacatgc gaattaattt tcacggcgcg gctcatacgg tgacgggcag
     1921 tcagcatttg ttggagatca atggaaaaaa actt
//
LOCUS       DAOQFT010000503         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_40394_length_1953_cov_3.688620, whole genome shotgun sequence.
ACCESSION   DAOQFT010000503 DAOQFT010000000
VERSION     DAOQFT010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40394_length_1953_cov_3.688620"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..802)
                     /locus_tag="PLR93_11345"
     CDS             complement(338..802)
                     /locus_tag="PLR93_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH27609.1"
                     /translation="MTQVIYGPRLGKEGNIRVGCSAVIFDETRQKVLLTQRADNGRWC
                     LPGGQLEAGESVAEACEREVWEETGLTVRAKRLAGVYSNPDQLVIYPDGNKAFFVVLN
                     FEVEIISGELGLSNETTAFGFYSLQEMESMPMHGEHKSRVEDAMLSANPVIK"
     gene            complement(847..1710)
                     /locus_tag="PLR93_11350"
     CDS             complement(847..1710)
                     /locus_tag="PLR93_11350"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNH27610.1"
                     /translation="MQTNHLHDMTNRTISHIIEPQLVIEGAGVLLRRSIAPHATNKFD
                     PFLLFDHFAFNNPLEGPLRGFPTHPHRGIETVTYMLEGTVRHRDSLGNAGTIGEGDVQ
                     WMTSGRGILHEEMPRRSERGNIYGFQLWVNLPAAQKMSQPRYQEVAGDTIPVVEKDGV
                     SIRLVAGELDGVRGPVTEIAAAPLYMDVKLAPGSRFSLSIPAGHTALAYVFEGVGEFA
                     DQAVEAVSMVVFQDDGDQIEVKSEAGVRFMLIAGAPFKEPIVPYGPFVMNTREEIEET
                     IRELRNGTFIK"
BASE COUNT          503 a          460 c          464 g          526 t
ORIGIN      
        1 caacatctgc acttagaatt attgtccgga gaggttctat attaaacgga atgacttcgt
       61 ttccaaaatg aaagattacg atggccatgg ctgcaataaa ccttgtaaaa gtgatctgtt
      121 ctatcctgaa attctgcatg tgttcctcat tgtaatcttt tgttttgtgt gaaatccgac
      181 atttaggaaa tttacagctt ctagttgatg atagagaact taatgtgaag tgttaccaaa
      241 gaacttttgg gtgaacttag ggtgatattt aatgcgtttg aaaacttaat ggagttaaat
      301 tgcccaatga cccttaaaag gaaaaaacat tggcctgtta ttttataaca ggatttgcac
      361 tgagcatggc atcctctacg cggcttttgt gttcgccatg catgggcatt gattccattt
      421 cttgcaggga ataaaaccca aaggccgtgg tctcattgct caagcccaat tctcctgaaa
      481 tgatctccac ctcaaagttc agtacgacga aaaaggcctt atttccatcc gggtaaatga
      541 ccaactgatc tgggttactg taaacacctg ccaaccgttt cgcgcgtaca gtcagaccag
      601 tttcttccca cacttcccgt tcacatgctt cagcaacgct ttcgcctgct tccagttgac
      661 cgccaggcaa acaccagcgg ccattatctg cacgctgggt gagcaatact ttttggcggg
      721 tttcatcgaa tatcaccgcg ctgcatccca cgcggatatt accttccttc cccaagcggg
      781 gaccgtagat gacctgtgtc atggtttaaa ttataagggc gactgcaaga gtcgcccata
      841 cgaaaattat ttgatgaatg tcccgtttcg caattcgcga atggtctctt cgatctcctc
      901 acgtgtattc ataacgaagg gaccgtaggg cacaatgggt tccttgaacg gtgctccagc
      961 aattagcatg aagcggacgc ccgcctcact cttaacctcg atctggtcgc cgtcatcctg
     1021 aaataccacc atgctgacgg cttccacggc ctgatcggca aactcgccca ctccttcgaa
     1081 cacatatgca agagcggtat gcccggctgg gatggagaga gaaaatcggg aaccaggtgc
     1141 caacttcaca tccatgtaaa gaggcgcagc agcaatctcg gtgacggggc cacggacgcc
     1201 atccaattcc ccggctacga ggcggatcga gacaccatct ttctcgacaa cgggaatcgt
     1261 gtctccggct acttcctgat aacggggttg actcattttt tgtgcggcag gcaggttgac
     1321 ccaaagctga aagccgtaga tatttccgcg ttcgctacgg cgcggcattt cttcgtgcag
     1381 aatcccgcga ccactagtca tccactgcac atcgccttcg ccgatggttc ctgcgttgcc
     1441 aaggctgtct cggtggcgta cagtaccttc gagcatgtaa gttaccgtct caatgccgcg
     1501 atgcgggtga gtgggaaaac cgcggagcgg gccttcaagc gggttattaa aagcaaagtg
     1561 gtcgaagagc aaaaaagggt cgaatttgtt ggttgcatga ggcgctatgg aacgccttaa
     1621 caatacgcct gccccttcga tcacaagttg tggttcgata atatgggata tggttctgtt
     1681 ggtcatgtcg tgtagatgat ttgtttgcat ataaacttac catccggaaa tacgccaata
     1741 gaatttgtga atgctgttta cctagcagat tccagaattg ctccgctaag cccatcaaga
     1801 atgacatgtt gagcatttat tttgtctttg ggtgaaagat ggaaaagaca atttgagctt
     1861 gagaaattaa tagtgctggg tttaggcgag aaattcaaaa agatggttag tgtcttgtta
     1921 ttatggattc tggagaagcc aagaatgtta cct
//
LOCUS       DAOQFT010000504         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_40495_length_1950_cov_4.772032, whole genome shotgun sequence.
ACCESSION   DAOQFT010000504 DAOQFT010000000
VERSION     DAOQFT010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40495_length_1950_cov_4.772032"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1341
                     /locus_tag="PLR93_11355"
     CDS             91..1341
                     /locus_tag="PLR93_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27611.1"
                     /translation="MLYDNAQLVRVYLHAWQVTHDPFYKRIVEETLEFIAKEMTHPQG
                     GFYSSLDADSEGEEGKFYVWSLDEIREVLKDETEFFEAAYGVTAKGNWEGNTVLQRAL
                     DDASLAARFKLDPEAVPAKLAESHSNLYTARSKRIRPGTDDKVLTSWNGLMLAAFAEA
                     ARVLAGVSPKAITYSNLATRSAEFLLSNLRVDGRLRRSWRDGKTTNEVFLEDYASLIL
                     GLLEMYQTDFNTKWFTAAKELADEMVEKFQDPNGGFFDTPIDGENLLFRPKDVQDNAI
                     PSGNSLACEALLKLAAYTDNGSYRDIAEKSFGLVHKFVLQYPLGFGRWLTAAQLATGT
                     LKQVAAVGEAGDPNFQSLLKVIWSEYRPNVVVAASSSLEKNTPALLHDRPLIHGKATV
                     YVCEGFVCQQPTTEADVLAEQLKN"
     gene            complement(1377..1556)
                     /locus_tag="PLR93_11360"
     CDS             complement(1377..1556)
                     /locus_tag="PLR93_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNH27612.1"
                     /translation="MGLPRGSEWIFILIIVVLVFGPGRIGKVAGEIGKSIKSFRDGLS
                     GEEEAEKKSDTTEQK"
     gene            complement(1615..>1950)
                     /locus_tag="PLR93_11365"
     CDS             complement(1615..>1950)
                     /locus_tag="PLR93_11365"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNH27613.1"
                     /translation="YRIIETAAKYLKLPLDKFIINVDRYGNTSTASIPIAAVEAIEKG
                     KLKSGNKVVFVGFGAGLTWGALVAQWSGPIPAKKQVHPEQYRLFARLRSWLLRALRFL
                     EGLLSRREL"
BASE COUNT          549 a          470 c          435 g          496 t
ORIGIN      
        1 cttagtgagc catcagacca taactaccaa ctgatatccg cgctgaaaac acaattccgt
       61 ttgtacgatt aggtccgcca ttacgagaaa atgctttacg acaacgctca actcgtccgc
      121 gtgtatttgc atgcctggca agtgacacac gacccattct acaaacgcat tgtcgaagaa
      181 actcttgaat ttatcgcgaa agaaatgact cacccacagg gcggttttta ttcttcgctc
      241 gatgctgact cggaaggcga agaaggcaag ttctacgtat ggagcttaga tgaaattcgt
      301 gaagtattaa aagatgagac tgaatttttt gaagcggcat atggagtgac cgccaaaggc
      361 aattgggaag gaaataccgt ccttcagcgt gcattagacg atgcctccct tgccgcccgc
      421 ttcaaactgg acccggaagc tgttcccgcc aagttagccg aatctcactc caatctttat
      481 acagcccgct ccaaacgcat tcgcccggga acagatgata aagtcctcac ctcatggaat
      541 ggattgatgc tcgctgcctt tgccgaagcc gcccgagtcc tcgcaggcgt tagtccaaaa
      601 gcaatcactt actcaaatct agctacacgc agcgcagagt ttttgttatc caacttgcga
      661 gttgatggca gattacgccg atcgtggcga gatggcaaaa ccaccaatga agttttcctt
      721 gaagactacg catcattgat ccttggacta ttggaaatgt accagaccga tttcaacacc
      781 aaatggttca ctgctgccaa agaactcgca gacgaaatgg tcgaaaaatt ccaagacccg
      841 aatggtggct tcttcgatac ccccattgat ggtgaaaacc tcctgtttcg cccaaaagat
      901 gtacaagata atgcaatccc ttcagggaat tccttagctt gtgaagcctt attaaagtta
      961 gccgcatata ctgacaatgg cagctatcgt gatattgcag aaaaatcatt tggcttggtg
     1021 cataaatttg tcttgcaata tccgcttggg tttgggcgct ggcttacagc ggcgcagctt
     1081 gctacaggaa cgctgaaaca ggtagcggcg gtgggagaag cgggagaccc aaacttccag
     1141 agtttgctca aagttatttg gtctgaatat agaccgaacg tggtggtggc tgcatcgtct
     1201 tctttagaaa aaaacacccc cgcattgctc catgaccgtc cactgataca tggtaaagcc
     1261 actgtgtatg tgtgtgaagg ctttgtttgc caacagccaa ctacagaagc tgatgtgttg
     1321 gctgagcagc taaaaaatta aacaaaaaac agaccgtgat tacggtctgt ttttaattac
     1381 ttctgttctg ttgtatcgga tttcttctct gcttcttctt cgccgctcaa accatcacgg
     1441 aaagatttga tgctcttgcc gatctcacca gctactttgc caatgcgacc cggaccaaaa
     1501 acaagaacaa cgataatcag aatgaaaatc cactctgaac cacgtggtaa acccatagta
     1561 cgctccttga ttgaatttca tatcaatcgt accatagttc aaatgaaaac ttgattagag
     1621 ttcgcggcga gagagcaagc cttcaaggaa acgcagggca cgtaacagcc atgaacgaag
     1681 gcgcgcaaac aaacgatact gctcggggtg aacttgtttc tttgcaggga tgggaccgct
     1741 ccattgtgca acgagcgcac cccaggttaa acccgcacca aagccaacaa aaaccacttt
     1801 gttaccgctt tttagtttgc ctttttcaat tgcttctact gcagcaatgg gaatagatgc
     1861 tgtggaggtg ttaccgtaac gatcgacatt gataataaat ttatctaagg gcagctttag
     1921 gtattttgcc gctgtttcaa taatgcggta
//
LOCUS       DAOQFT010000505         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_40619_length_1947_cov_5.445032, whole genome shotgun sequence.
ACCESSION   DAOQFT010000505 DAOQFT010000000
VERSION     DAOQFT010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40619_length_1947_cov_5.445032"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PLR93_11370"
     CDS             <1..896
                     /locus_tag="PLR93_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNH27614.1"
                     /translation="DEIILVSEALHEQGIADTAQQIAEKNTRIILIAGPSSSGKTTSS
                     RRLAVQLLARGISPYPLELDNYFVDREKTPRDENGEHDFETIEALNLERLARDIEKLI
                     SGEEVQLPRYNFKTGRSEEGDVVRLREGQPLILEGIHGLNPRLIPDRWADSAFRLYVS
                     ALTQLNLDRHNRVSTTDTRLIRRIVRDARERGYSAQATISRWESVRRGEKRHIFPYQE
                     NADVMFNSALVYELSALKPLVEPLLRQVPYDATEYIEARRLLAFLDWFLPLDANLIPM
                     NSIVREFIGGSSLKDFKIWRG"
     gene            complement(955..1944)
                     /locus_tag="PLR93_11375"
     CDS             complement(955..1944)
                     /locus_tag="PLR93_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH27615.1"
                     /translation="MNYLPSTSRYQSMHYRRSGRSGLKLPAVSLGLWHNFGGVDVYEN
                     SRAMVLRAFDLGITHFDLANNYGPPPGSAEETFGQIMAKDLHPWRDELFISSKAGYYM
                     WEGPYGEWGSRKYLVSSLDQSLKRMGLEYVDLFYHHRPDPDTPLEETMQALDFIVRSG
                     RALYVGISNYPADKAREASQILKSLGTPCLIHQPKYSMFERWVEDGLLQTLKEEGIGC
                     IAFSPLAQGLLTDKYLGGIPEGSRASKAHGFLKPAHITDERIAKVQKLNTLAQQRGQT
                     LAQMALAWVLRHEGMTSVLIGASKVSQIEDAVGMLNKVEFSGDELQAIENILR"
BASE COUNT          462 a          495 c          501 g          489 t
ORIGIN      
        1 ccgatgagat tatccttgtg tctgaggcat tgcatgaaca aggcattgcc gatacggctc
       61 aacagattgc agaaaaaaat acgcgcatca tcctgatcgc gggtccatcc tcctctggca
      121 agaccacctc ttcgcgccgc cttgcggttc agttattggc gcgtggcatc tcaccgtacc
      181 cgcttgagtt ggataattat tttgtagatc gtgaaaagac tccgcgtgac gaaaatgggg
      241 agcatgattt tgaaaccatt gaagcattaa atcttgaacg gctggctcgc gacattgaaa
      301 aacttatcag cggcgaagaa gttcaacttc cgcgctacaa cttcaagacc ggaagaagcg
      361 aagaaggaga tgtggtccgg ctgcgagagg gtcaaccgct catcctcgaa ggtatccatg
      421 gattgaaccc gcgcctcatc cccgaccgtt gggctgattc agcgtttcgg ctttacgtct
      481 ccgccctgac ccagcttaac ctagaccgcc ataaccgcgt ttccactacc gatacaagat
      541 tgattcgccg tattgtgcgc gatgctcgtg aacgcggata ttctgcccaa gccacgatct
      601 cgcgctggga gtcggtgcgg cgcggcgaga agcgccatat tttcccatat caagaaaatg
      661 ccgatgtgat gttcaattct gcgttggtgt atgagctttc ggcgttgaag ccattggtgg
      721 agccgttgtt gcggcaagtg ccatatgatg ccactgaata cattgaagcc cgccgtttgc
      781 tcgccttcct cgattggttc ctgcctttgg atgcgaactt gatcccgatg aattccatcg
      841 tgcgtgaatt tatcggcggc tccagtttga aagatttcaa gatttggcgt gggtgatttt
      901 taaatgcagg catccgaggt tttcaaaacc tcggatgcct ttttgtttaa ttctttacct
      961 taagatattt tcgattgctt gcaactcgtc ccctgaaaat tccactttat tcaacatccc
     1021 aacggcatct tcgatctggc tgaccttgct tgcaccgatc aacacagagg tcatgccttc
     1081 atggcgcagc acccaagcca gcgccatttg tgcgagggtt tgtccgcgtt gctgggcgag
     1141 agtgttgagc ttttgaacct tggctattct ttcatcggtg atgtgagccg gtttgagaaa
     1201 gccatgcgcc ttcgaagcgc gtgacccttc aggaatgccg ccgagatatt tatcagtaag
     1261 taagccttgt gccaacggag agaaagcaat acagccaatg ccttcttctt tcaaagtctg
     1321 aagcagacca tcttccaccc agcgctcgaa catactgtat ttgggctgat gaatcaaaca
     1381 aggcgtaccc aaggacttga gaatttgaga cgcttcacgg gctttatcgg cagggtaatt
     1441 tgaaatgccc acatacaacg cccgtccact gcggacgatg aaatcgagcg cttgcatcgt
     1501 ttcttcaagc ggagtatcag ggtcggggcg gtgatgatag aacaagtcta catattccaa
     1561 gcccatgcgc ttgagacttt ggtctaaact ggaaacgaga tacttgcgcg agccccattc
     1621 tccgtatgga ccttcccaca tgtaataccc ggctttcgat gagataaata attcatcgcg
     1681 ccagggatgc aggtcttttg ccatgatctg cccaaaggtc tcttctgccg agccgggagg
     1741 cggaccataa ttattggcaa ggtcaaagtg agtgataccg aggtcgaagg cgcgcagcac
     1801 catggcgcgg ctgttttcgt acacgtccac gccgccgaag ttatgccaca aaccaagcga
     1861 aacggccggg agttttagtc cgctacgccc tgagcggcgg tagtgcatgg attgataacg
     1921 agaggtggaa gggaggtaat tcataaa
//
LOCUS       DAOQFT010000506         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_40921_length_1939_cov_6.054671, whole genome shotgun sequence.
ACCESSION   DAOQFT010000506 DAOQFT010000000
VERSION     DAOQFT010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40921_length_1939_cov_6.054671"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PLR93_11380"
     CDS             complement(<1..1292)
                     /locus_tag="PLR93_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27616.1"
                     /translation="MKILLATKLTAKFLLPIFASAILILTQAGKGILNLTDAPAPELH
                     PEIKDSSSSFDTLRSATQPAQPTQASVIADTISYQIEAPVLPAYPFANPAGCSAAQPN
                     LTPEQMLTNVQNAIGNEYNLAERRQLYVWNGEQGSMLNVTNDPFVYEFTFNEFQHPLT
                     YRGDKVATIFMTQGFVVWFREYSDSFRLTAIPMTNGVMQSAWAPYVTSYWQQNGQPND
                     EKIYPVMKKLPCHWVIDSGYVTNETLTTMFNLDWHTPDYLSAGRQYLASTCKEAYQIS
                     QEKVGYWDATSMCGPLAWTIMRDVNAFPYRIGSWSQNAATFTRVNPRWDGQPWGSFDP
                     ETFTLFHTEEAIAGYDFQSKGNLYTGDVVYTFATLYVTAGYFDHIFVVAGVDENNTRL
                     AISNMVQNSPYADCSIEEVKLYTPGDRETGVLNHEWNG"
     gene            1525..>1939
                     /locus_tag="PLR93_11385"
     CDS             1525..>1939
                     /locus_tag="PLR93_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH27617.1"
                     /translation="MSKKKTSSELEKELLDAKQRIAELEQNEARFATILENSPVAIGI
                     SRLSDLKLIYVNSAFADLFGYSQDEILKNSTVDLGIWKDLEDRQKFFEIVRNKNKVTG
                     FEMSVRPQQGSEKNILVKGEMVEIDKQPCFVSQVID"
BASE COUNT          504 a          363 c          536 g          536 t
ORIGIN      
        1 tttccattcc attcatggtt gagaacgccg gtctcgcggt cgccgggggt gtagagtttt
       61 acttcttcaa tcgagcaatc tgcatacggt gagttctgca ccatgttcga gattgccaag
      121 cgggtattgt tctcgtccac accagcgacg acaaaaatat ggtcaaagta tccagcggtt
      181 acatataggg tggcaaaggt gtaaaccaca tcgcctgtgt agagatttcc tttagattga
      241 aaatcataac cggcaatggc ttcttctgta tggaagagag tgaaggtctc tgggtcgaac
      301 gatccccagg gttgtccgtc ccagcggggg ttcacgcggg tgaatgtcgc agcgttctgt
      361 gaccaacttc cgatgcgata agggaaggcg tttacatcac gcatgatcgt ccatgccaaa
      421 ggtccgcaca tcgaggtggc gtcccagtag ccaacttttt cctgagagat ttggtacgct
      481 tctttgcaag tgcttgctaa atattgacga cccgccgaaa gataatcggg agtgtgccaa
      541 tctaggttga acatggtggt gagagtttca ttggtcacat aaccagagtc gatcacccaa
      601 tggcatggaa gttttttcat gaccgggtag attttttcgt cgttgggttg cccgttctgt
      661 tgccaatagg aagtgacata aggtgcccac gcagattgca tgacgccgtt ggtcattggg
      721 attgcggtaa ggcgaaaact atcgctatat tcgcggaacc aaacgacgaa gccttgggtc
      781 atgaagatgg tggcgacttt gtctccgcga taggtgagcg gatgctgaaa ttcattgaag
      841 gtgaattcat aaacaaacgg gtcattcgtg acgtttagca tgctgccttg ttccccgttc
      901 catacataca gctgacggcg ttcagcgagg ttgtactcat tgccgatggc attttgtacg
      961 ttggtaagca tttgttcagg agtgaggttt ggctgggcag cggagcaacc ggcagggttg
     1021 gcgaaaggat aagcaggaag caccggggct tcgatttggt aggaaatcgt atcggcgatg
     1081 acggaagctt gggtaggttg agcaggctgg gtcgcggaac gaagcgtgtc gaaacttgag
     1141 gaagagtctt taatttcagg gtgaagttca ggggcggggg cgtcggttaa gttgagaatc
     1201 cccttgccag cttgggtgag aataaggatt gcgctggcaa atatggggag aaggaatttt
     1261 gcggtaagtt ttgtagcgag aagaattttc atgccatcaa ccttttgtgt tggcggcatt
     1321 atttcattta aggctgggtt tcctcagacg tgttctctag tatttcttta gtaatttttg
     1381 cctaattttg cgtgggtaaa ttcctgccaa tgagatggtt gatttacgca acttacaggt
     1441 agggaattcc cccattctct cgttttcgat actatgcaaa aatatattaa gagtgtttct
     1501 atcagggttt ataggaagta aattatgagc aaaaagaaga ccagttctga actggaaaaa
     1561 gaattattag atgctaaaca gcgcattgca gagctggaac aaaatgaagc gcgttttgct
     1621 actattttag agaatagccc agttgcaatt ggaatttcac ggctgagtga tctgaaactt
     1681 atctatgtga attcggcgtt tgcagatctg tttggatatt cacaagatga aatactgaaa
     1741 aactctacgg tagatttggg aatatggaag gatcttgaag atcggcagaa gttttttgaa
     1801 atagtacgca acaagaataa agtgacgggt ttcgagatga gcgttcgccc acagcagggt
     1861 agtgaaaaaa atatccttgt taaaggggag atggtcgaga tcgataaaca accttgtttt
     1921 gtatcacagg tcattgata
//
LOCUS       DAOQFT010000507         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_41033_length_1936_cov_7.040404, whole genome shotgun sequence.
ACCESSION   DAOQFT010000507 DAOQFT010000000
VERSION     DAOQFT010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41033_length_1936_cov_7.040404"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLR93_11390"
     CDS             complement(<1..292)
                     /locus_tag="PLR93_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNH27618.1"
                     /translation="MAIRQIVHLPEPILRKKAKPVTKFDKDLQVLIDDMIETMREAPG
                     VGLAAPQINLPMQLAVVEYAEGEDEEREEGAPEPKKKQFVLINPEIVKVSEEK"
     gene            complement(386..>1936)
                     /locus_tag="PLR93_11395"
     CDS             complement(386..>1936)
                     /locus_tag="PLR93_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27619.1"
                     /translation="ILFLSRQGFDGLLKQYETLKPNFQVSIKSRKLARSTRFKWLGKN
                     EVIYFIARRHKIRLFQALVTPFFSLLGALALIALGFFVSSVIPAAVGVVLVIGVLGWG
                     VWNAIDWSNDYYIVTNQRVVWLEKIIFLHDSREEAPLGTILSVGVETDALGRILKYGN
                     VIVRTFVGKLEFEYVDHPQQAADMIREYWERTKAITTRTQMDVMKDTIKQKLGLPLDK
                     RPQVELSPVVPMDEEQVLKTPLWMLALQNLFQLRLEEGGKIIYHKHWIILLQQGWRPF
                     GLFFLGIILFIWRMVVLYNSPDKAFITFNGGFSFKPDTISVVIPLLLLPVAGWVWYEY
                     TDWKNDTFMVTQDEIFDIDRKPFGTEERRSAQIENILSTSYKRKGFIAYMFNYGTVNI
                     TVGGTQMAFEDVMDPAAVQADINRRRAQRIAKKNEDAGSADRERFATWIAAYHQHIGE
                     FNKPIVPPANANVSNTNPFAAVSKEKDLDDLPIKDDDDFDMDDFGDDDFDGDDDFGGD
                     GDGGGGDN"
BASE COUNT          478 a          484 c          451 g          523 t
ORIGIN      
        1 ctttttcttc agagactttc acgatctcag ggttgatgag cacgaattgt tttttcttgg
       61 gttcgggtgc gccttcttct cgctcttcat cttcgccttc cgcatattcc accacggcta
      121 attgcatggg caggttgatc tgaggcgcgg ctagacctac acccggcgct tcacgcatgg
      181 tctcgatcat gtcgtcgatg agtacttgta aatctttatc gaattttgta acgggttttg
      241 ccttcttccg caaaatgggt tcgggcaggt gaacgatttg tcgtattgcc atttctttcc
      301 tcttttaaat tccatgcgta ttgacgctta acttttcaat agggcggcaa tgatacttgg
      361 attgtctttt ccccgctacc ctgagttagt tatctccgcc gccaccgtct ccgtcaccgc
      421 caaaatcgtc atcgccatca aaatcgtcgt caccgaagtc gtccatatcg aaatcgtcat
      481 catctttgat tgggaggtcg tccagatctt tttcctttga gacggcggca aacggattgg
      541 tgttgctgac gttcgcattt gctggcggga cgatgggttt attgaactcg ccaatatgtt
      601 gatgataggc tgcaatccaa gtggcgaaac gttcacggtc ggcgctgccg gcatcttcat
      661 ttttcttggc aatgcgttga gcccgccgac ggttaatgtc cgcttgcacg gcagcagggt
      721 ccatcacatc ttcaaacgcc atttgagtac cgcctacggt gatgttaact gtcccgtaat
      781 tgaacatgta ggcaataaac cccttgcgct tgtaagatgt gcttaagatg ttctcgatct
      841 gagcagagcg gcgttcttct gtgccgaaag gtttgcggtc aatatcgaat atctcgtcct
      901 gggtgaccat gaaggtatcg ttcttccagt cggtgtattc ataccaaacc caacctgcta
      961 caggtaataa caaaagtgga atgaccacag agatcgtatc gggcttgaag gaaaagccgc
     1021 cattgaaagt aataaaggct ttgtcgggcg agttatacaa caccaccata cgccaaataa
     1081 aaagaataat gccaaggaaa aagagcccga atggacgcca accttgctgc aacaaaatga
     1141 tccaatgctt gtgatagatg attttgccac cctcttcaag acgcaattga aagaggttct
     1201 gaagtgccaa catccacaat ggcgttttga gcacctgttc ttcatccatg ggcactacag
     1261 gagaaagctc cacttgcgga cgtttatcca acggcaagcc tagcttctgc ttgatggtgt
     1321 ctttcatgac atccatttga gtgcgggtgg tgatggcttt ggtacgttcc caatactcac
     1381 ggatcatgtc cgcggcttgc tggggatgat cgacgtattc aaattccagc ttgcctacga
     1441 aggttcgcac gatcacattg ccatatttca gaatgcgtcc cagtgcatct gtctcaacac
     1501 cgacggataa gatagttcct aacggcgctt cttcgcggct atcatgcaag aagatgattt
     1561 tctccaacca cacaacacgt tgattggtga cgatgtagta atcgttgctc cagtcgatgg
     1621 cattccacac tccccagcct aaaacgccaa tcaccagcac cactcccacc gccgccggga
     1681 tgaccgaact tacgaagaag cctaaagcga ttaatgccag cgcgccaagc aaagaaaaga
     1741 atggcgtgac caatgcctga aagaggcgaa ttttatgtct gcgagcaatg aagtaaatga
     1801 cttcgttctt ccccaaccac ttaaagcgtg ttgaacgcgc aagtttgcgg ctcttaatgg
     1861 aaacttgaaa atttggcttg agggtttcgt actgtttaag cagcccgtca aaaccctgac
     1921 gtgaaagaaa tagaat
//
LOCUS       DAOQFT010000508         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_41273_length_1930_cov_4.904533, whole genome shotgun sequence.
ACCESSION   DAOQFT010000508 DAOQFT010000000
VERSION     DAOQFT010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41273_length_1930_cov_4.904533"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="PLR93_11400"
     CDS             complement(<1..1813)
                     /locus_tag="PLR93_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH27620.1"
                     /translation="MNNTSAASPQNNQFGLWANLPLSRKILLAFSVLFISNVIIAIIT
                     LQGMNRTQTAFEHAITQGLEIHALSDHLTISFLQARRAEKNFLLRWREEGFDTAYANY
                     IPLFDENVAAARADVKKLAAFGPVVATTGDYTQTQYNADIEALNQNISIYEQGFKAYV
                     DTLQQKGFDENTGFEGEFRTIARNIEGKISGGDGLEELEITLLQIRRNEKDYLLRTDQ
                     QYIDNVHSNISKLKTQIANTDKLTPTIKSDLLSYTDEYLTAFDSLIELDKKITIYNEN
                     LISSARSVEPLVAKIEGFGEQLATEDIDAARLNVSQTQTTSTAIVFGMLVISIFLAVG
                     LSRQITRPIIQLTSTAEVISTGNFDAQAKVDSSDEIGTLAQTFNTMTNRLNQALEDVR
                     RRALAVQTSAEVSQRLSVATDPQRLAVEVVEQVQAAFKYYHAHIYFLDEESGDLIMAG
                     GTGDAGATMLARGHKIPHGRGLVGRAAATNAPVLVPDVSQAEGWLPNPLLPDTKSEVA
                     IPISSGNQILGVLDVQQNIVNGLDEEDVRLLQSLAGQVAISLQNARSYEQSKSQAELE
                     TLANTIGQKIQRANTVEETLQTAIRELGLALGATRVKA"
BASE COUNT          438 a          411 c          503 g          578 t
ORIGIN      
        1 ctgctttgac gcgggttgca cccaatgcaa gcccaagttc gcggatggcg gtttgtaggg
       61 tctcttccac ggtgtttgcg cgttggatct tctggccaat ggtatttgcc agcgtttcca
      121 actcagcctg cgatttggat tgttcgtacg agcgtgcgtt ttgtaaagag atcgcgacct
      181 gcccggcgag ggattgtagt aatctaacat cttcctcgtc aagaccattg actatatttt
      241 gctgcacatc caacacaccc agaatctgat tgcctgaaga gatcggaatg gctacttcag
      301 atttggtatc tggtaataag gggttgggga gccagccttc ggcttgtgag acatccggta
      361 ccaacacagg agcattggtc gcagcggcgc ggcccaccaa accacggcca tgagggattt
      421 tgtgaccacg tgcaagcatg gttgcgcctg catcgccggt gccgcctgcc atgatgaggt
      481 ctccactttc ttcatcgagg aagtagatat gggcgtgata gtatttgaac gcggcctgca
      541 cctgttccac cacttcaaca gcaaggcgtt gtgggtcggt ggctacggag aggcgttgac
      601 tgacctctgc agaggtttgt acggccaatg cacggcggcg gacgtcttca agtgcctgat
      661 ttaggcggtt tgtcatggtg ttgaatgttt gtgcgagagt accgatttca tctgatgagt
      721 cgacttttgc ttgtgcatca aaattaccgg ttgaaataac ttcggcagtg cttgtgagtt
      781 ggatgatcgg gcgggtgatt tgacgggaaa gaccgactgc cagaaaaata gatatgacaa
      841 gcatgccaaa tacgattgca gtggaagtgg tttgtgtttg acttacattc aatcgtgcgg
      901 catcgatatc ttctgtagcc aattgttcac caaatccctc gatctttgcg accagtggtt
      961 ctacactacg tgcggagctg atcaaatttt cattgtatat ggtgattttt ttatcaagct
     1021 ctataaggct gtcgaaggct gtcaggtatt catcagtata agaaagtaga tcgcttttta
     1081 tggtaggtgt gagtttgtcg gtattggcga tttgggtttt tagtttggaa atatttgaat
     1141 ggacattatc aatgtattgt tgatctgtac gcagcagata gtccttttcg ttgcgacgaa
     1201 tttgaaggag ggtaatttcc agctcttcca gaccatctcc gcctgaaatt tttccttcga
     1261 tatttctggc aattgttctg aattctccct caaaacctgt gttttcatca aacccctttt
     1321 gttgtagtgt atctacataa gccttaaaac cttgttcgta tatggatatg ttctggttga
     1381 gggcttcaat atcagcatta tattgtgtct gagtgtaatc gccggtagtg gctactacag
     1441 gcccaaaggc cgcgagtttt tttacgtctg cacgtgccgc agcaacattt tcatcgaaca
     1501 agggaatgta gtttgcatag gccgtgtcga acccttcttc acgccagcgt aacagaaagt
     1561 ttttttcagc gcggcgggct tgcaaaaagc tgatggtaag gtgatctgat agtgcatgga
     1621 tttccaggcc ttgagtaatt gcatgttcga aagcggtttg tgttctgttc atgccttgca
     1681 atgtgatgat ggcgattatc acattggata tgaataatac gctgaacgca agaaggatct
     1741 ttcgcgatag tgggaggttt gcccaaaggc caaattgatt gttttgtggc gaagctgcag
     1801 aagtgttgtt catgtaaaga ttccttaatt cccttcgctt tcacctgcga ttgacctggg
     1861 ttcaagcgtt acaactgtgg atttcatgcc catggcatgc cccaattcac gtgctgcaac
     1921 ctgcaaggca
//
LOCUS       DAOQFT010000509         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_41674_length_1919_cov_6.441524, whole genome shotgun sequence.
ACCESSION   DAOQFT010000509 DAOQFT010000000
VERSION     DAOQFT010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41674_length_1919_cov_6.441524"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="PLR93_11405"
     CDS             complement(<1..1875)
                     /locus_tag="PLR93_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNH27621.1"
                     /translation="MNEFDKNKKLETLLEETAEQLQPNVMFKAELEEKLRKAHKPRAS
                     LPTFAFRNWLPALTGIVVVGGLVIFMLWLFQTLEPQKSVGTNEEQPIEVQTTPGVIID
                     PNNTNSNETIGGYDYRGGKLFLTSALPESSATANIYEYIADQPATIEEARALANQFGL
                     QGEVYTTAFPQAPDKIGYVISDGKRMLTVYTKNYFTYTPDILVTDRGYYGVNNDNAET
                     VISDFFNQNGLTFPFSISKSPAKGMYHFMQLSPDSIPMQYDIYTIQSSNVTLGQNGEV
                     AYLSANMLSYNPQAFGSYGIISAEDALNAILDPHSTVGMTESGSGGEGGVIPQQWYRE
                     YPNNETVSISGSLIKYDSATAGQPPLIFVGSFEALGNTTDLQALSNFAFVQVTGQFVE
                     ENGIRKFNVESTNANVIQGYITGSLRKEGDQIILTSDDGNSEYVLLDPPADLPLNTVP
                     TESYLGISGILVDNTLDWSNIQYVADASSMGGGGGGGGFGFYPLNLSGTPAVFPTPIP
                     AEEQYSAAEVASFLNYIVENGDTLESIAAKFNVSLEDLMRVNYISDMNVIETGWLIKI
                     PGVPGPTQLTGEEGIVSISKYIKPDGRVRYQYTFLSSQSYNGIYYELQGDNLEPLQNL
                     V"
BASE COUNT          467 a          438 c          451 g          563 t
ORIGIN      
        1 aaccaagttt tgaagtggct caagattatc gccttgcaat tcgtaataga tgccattgta
       61 cgactgtgag cttaagaagg tgtattgata ccgcactctg ccatctggtt tgatgtactt
      121 cgagatgctc acaattcctt cttcgcctgt tagttgagta ggacctggca cgcctggaat
      181 tttgatgagc cagcctgttt caatgacgtt catatctgaa atgtagttca cgcgcatgag
      241 gtcttccaac gagacattga atttggcagc gatgctttcc aaggtgtcac cattctctac
      301 aatatagttc aagaagctgg cgacttctgc tgcactgtat tgttcttctg ctggaattgg
      361 tgttgggaaa acagcaggag tgccgcttaa gttgagcgga tagaaaccaa acccgccgcc
      421 tccgccacct ccgcccatgc tactggcatc tgcaacatat tggatgtttg accagtccag
      481 agtgttatcg accaaaattc cagagatacc gagatatgac tccgtaggca ccgtattaag
      541 gggcaagtct gcgggtgggt ctagcaaaac atactcgctg ttgccatcgt cactggtaag
      601 gatgatctga tcgccttcct tgcgcagcga gccggttata tatccctgaa taacattggc
      661 gttcgtgctt tccacattaa atttgcggat gccattttcc tcaacaaatt gacccgtcac
      721 ttgtacaaag gcaaagttgc tcagagcttg taagtctgtg gtgtttccaa gtgcctcaaa
      781 actgccaaca aaaatcaacg gtggctgacc tgctgtcgca gagtcgtatt ttatgaggct
      841 gccagagatt gaaacggtct cattatttgg atattctcga taccattgtt gtggaatcac
      901 gccgccttct ccgccagagc cgctttcggt catgccgacg gtgctatgtg ggtcgaggat
      961 ggcattcaag gcatcttccg cgctgatgat gccatagctt ccgaatgctt gtgggttgta
     1021 gctgagcata tttgcagaga gataagctac ttcaccattt tgtccaagcg tgacatttga
     1081 actttggatg gtgtagatgt catattgcat ggggatgcta tctggcgaaa gttgcatgaa
     1141 atgatacata ccctttgcag gagacttgct gatgctgaat gggaacgtta acccgttctg
     1201 attgaagaaa tcagaaatga ctgtctctgc attgtcgtta ttcacaccat agtaaccgcg
     1261 gtctgttacc aaaatatcgg gtgtgtatgt gaagtaattt ttggtataca cagtaagcat
     1321 tcgcttgcca tctgaaatga cataaccaat cttatcgggc gcttgtggaa atgcggttgt
     1381 atacacttca ccttgtaacc caaattgatt tgcaagcgct cgcgcctctt caatggtagc
     1441 gggctgatca gcaatgtact catagatatt ggcagttgct gaactctcag gcaaagcaga
     1501 tgtaaggaac aacttcccgc cgcgatagtc atatccgcca atggtttcgt tgctatttgt
     1561 attattcggg tcaatgatga cgcccggagt tgtttgcact tcaataggtt gttcttcgtt
     1621 agtgccaacg cttttttgtg gctccaacgt ttggaagagc caaagcataa aaattaccaa
     1681 tcctccaaca acgacaatac ctgtcaacgc gggcaaccag tttcgaaatg caaaggttgg
     1741 caaacttgcg cgtggcttat gcgccttacg aagcttctct tccaattcgg ctttaaacat
     1801 tacattgggt tgtagctgct cggcagtttc ttccaataac gtttcgagtt ttttgttttt
     1861 atcaaattcg ttcattcttc cacctccatt ctgagggagg ttcgctcgcg caggtcttg
//
LOCUS       DAOQFT010000510         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_41876_length_1914_cov_3.568047, whole genome shotgun sequence.
ACCESSION   DAOQFT010000510 DAOQFT010000000
VERSION     DAOQFT010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41876_length_1914_cov_3.568047"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLR93_11410"
     CDS             <1..206
                     /locus_tag="PLR93_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27622.1"
                     /translation="SDVLVEKLNKPDQIETIQRLIKDHFHVDLSVRCVVTNVKGKLPA
                     HVAQDGMVAAAIQHGGEIVDMDE"
     gene            248..691
                     /locus_tag="PLR93_11415"
     CDS             248..691
                     /locus_tag="PLR93_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27623.1"
                     /translation="MATSNKNNSGKKKDVKIEFTISPVLQQVEKKISEAQNFQLEVIA
                     RTNFNSFNGKRIAEWLRENHRMWRSVLLPLDFISLRDMTDGHWHADTLYIYPEDGYGF
                     KLEEIMKEQFHADETQWIGRESAMDMLGTSKIEDKSYVILSAWWD"
     gene            744..1073
                     /locus_tag="PLR93_11420"
     CDS             744..1073
                     /locus_tag="PLR93_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNH27624.1"
                     /translation="MAKGFNKGPAMGGQAGMMQQLQRMQKQMEEAQAKLAEETVTVTT
                     GGGAIKVVMTGDQKCKSVEISPEFLKDADAEMLQDMVLSAVNMALDQSRDLQQKLMGP
                     LAGGLPF"
     gene            1143..1748
                     /gene="recR"
                     /locus_tag="PLR93_11425"
     CDS             1143..1748
                     /gene="recR"
                     /locus_tag="PLR93_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNH27625.1"
                     /translation="MLLPESIQNLVTALERLPGIGPKSASRLAFFLLRASEDVAQDLS
                     TALANLKSNTAFCSECFNITDAGRERCEICESQKRDSSLICVVEEALDVLALERTGGF
                     QGKYHVLQGVLSPIEGIGPDDLKIKQLAERVARGGVGEVIIATNPSMEGDATALYLRQ
                     QLEPMGVKVTRLARGLPVGGDLEYADQNTLLRALAGRHEMN"
BASE COUNT          516 a          469 c          470 g          459 t
ORIGIN      
        1 caagtgacgt gctcgtggag aagctcaaca aacccgacca gatcgaaacc atccaacgcc
       61 tcatcaaaga ccacttccac gtggatttgt ccgtgcgctg tgtcgtcacc aacgttaaag
      121 gtaaactccc cgcacacgtc gcgcaagatg gcatggtcgc cgccgcgatc caacatggtg
      181 gcgagatcgt ggatatggat gagtagagcg gtaattggag attagtaatt ggtaattgag
      241 gctaattatg gcgacttcga ataaaaataa cagcggcaag aaaaaagatg taaaaattga
      301 atttacgatc agccctgtgc ttcagcaagt agagaagaaa ataagtgagg cacaaaattt
      361 tcaattagaa gtcattgccc gaacaaactt caattcgttc aatggcaaaa gaatcgctga
      421 gtggctaaga gaaaatcacc gaatgtggcg gtcagttttg ttgccgctgg attttatttc
      481 tctccgtgat atgacagatg ggcactggca tgcagacaca ctttatattt accctgaaga
      541 tggctatggc tttaaattag aagaaattat gaaagaacaa tttcacgccg atgaaaccca
      601 atggattggg cgagagagtg caatggatat gcttggcaca agtaaaatcg aagataaatc
      661 gtatgtgatt ctttccgctt ggtgggactg aatctccaat atccaatctc taattaccaa
      721 ttacttaact aggagactat tttatggcaa aaggattcaa caaaggaccc gccatgggcg
      781 gacaggctgg catgatgcag caattgcaac gcatgcaaaa gcaaatggaa gaagcacagg
      841 cgaaactcgc cgaagaaacc gtcaccgtca caaccggtgg gggagcgatc aaagtcgtca
      901 tgaccggtga ccagaaatgc aaatcggttg agatttctcc tgaatttctc aaagatgccg
      961 atgccgaaat gctgcaagac atggtcttat ctgctgtaaa tatggcgctt gaccaatctc
     1021 gcgaccttca gcaaaagttg atgggtccgc tggcgggtgg gcttcccttc tagtgttcca
     1081 agttccacgt tccaactgtc aggttgcttg gaacgtggaa cttgacactt ggaacacctt
     1141 ttatgctgct acccgaatcc atccaaaatt tagtcaccgc cctcgaacgc ctgcccggca
     1201 tcggacccaa gtccgcttca cggctggcat tctttctttt gcgcgcctcc gaagatgttg
     1261 cgcaagatct gtctacggcg ctggcgaatc tcaagtccaa taccgctttt tgctcggaat
     1321 gtttcaacat caccgatgcg ggacgtgaac gctgtgagat ctgcgaatcg caaaaacgcg
     1381 actcgtctct tatctgtgtt gtcgaagaag ctttagacgt tcttgcactc gaacgcactg
     1441 gcggctttca aggtaagtat catgtcttgc agggagtttt atctcccatc gaaggcatcg
     1501 gtcccgacga tctgaagatc aagcaactcg ccgaacgcgt agcgcgtggg ggagtggggg
     1561 aggtcatcat cgccaccaat cccagcatgg aaggtgacgc cactgcactt tatcttcgtc
     1621 aacaacttga gccgatgggt gtcaaagtca cccgccttgc gcgcggtctg cccgttggcg
     1681 gcgatctgga atacgccgat caaaatacct tgctgcgtgc actggcaggc agacacgaaa
     1741 tgaactaatc cctgatgggg cgcgagaacc gcgcccctac ggttttaaaa atgaacatca
     1801 ccatcctcac ttttggttct cgcggcgatg tccaaccgtt tttaacttta tctctcggtt
     1861 tgatgtctcg cggacataaa gtcatccttg ctgcgcctgc gcgcttcaaa tcac
//
LOCUS       DAOQFT010000511         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_42264_length_1903_cov_5.352273, whole genome shotgun sequence.
ACCESSION   DAOQFT010000511 DAOQFT010000000
VERSION     DAOQFT010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42264_length_1903_cov_5.352273"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1748)
                     /locus_tag="PLR93_11430"
     CDS             complement(171..1748)
                     /locus_tag="PLR93_11430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH27626.1"
                     /translation="MNIGRYEVIRELGQGGMAIVYLAYDPNIKRQVAIKVLPKQFTFE
                     PQFRARFQHEVEVVAKLENAAIVPVYDSGEHEDQPYIVMRYMPGGTLADRLGNGPIAL
                     TEIEPLFQRIGSALDYAHRSGVVHRDIKPGNILFDSQGEAFLSDFGIAKLAETSVAFT
                     GTGNMVGTPAYMSPEQAMGEKNIDGRSDIYSLGVVLFEALSGKLPFVSDTPMGVAIAH
                     IQEPVPSLLDHNPNLPQSFETIIRKAMEKKPAERYQTASELSQAIRSMGKSSSPGTMI
                     EQPISSTIIESHASPSRVAVPAPNLSSTPHPTPVSKKSIFPKVLGGIGLMGLCLCVVL
                     TGVFASSLLPGLFTTSSTQVSAETPFASATEEPAPTVFVPIVPTTMANAEIAAEGTVD
                     VSSIYDSTFPASLAIDGDPTTSWFSAGPDEDGTTRYVWTGVQEDFIATIDLISNKDNE
                     VAEFRSGYGFESVLIQVYDAQDQLVYEETVSLAGTPDPDIQITPNVVGQWIWIIFSGS
                     EAQDRGGFAELKVGVVR"
BASE COUNT          442 a          429 c          511 g          521 t
ORIGIN      
        1 gtgttagcac gttacccgtc tcagacgcct gtggttgatg ttcttgcacc ggtgtttacg
       61 gcgattaagt gggtctttgt gaatggaagt tttgttgttc ttctcattgg tatgttgttg
      121 ttcttgtgga aacgcttgaa aaaatagatg gcgcggaacc tgaaaagttt ttatcttacg
      181 acgccgacct tcagttcggc aaaaccaccg cgatcttgtg cctcgctgcc agaaaagata
      241 atccaaatcc attgcccgac gacgttggga gtgatctgaa tatctgggtc aggcgttcct
      301 gccaagctga cggtctcttc gtagactagt tgatcttgag cgtcgtacac ttgaattaac
      361 acagattcaa aaccgtagcc gctgcgaaat tcagccactt cattatcttt gtttgaaatt
      421 agatcgatgg tggctataaa atcttcctgg acgcctgtcc acacatatcg ggttgtgcca
      481 tcttcatcgg gtccggcaga gaaccatgaa gtagtggggt ctccatcaat ggcaagggag
      541 gcagggaagg tggagtcgta aatcgaggaa acatctacag tgccttctgc ggcgatctcg
      601 gcatttgcca tggtagtcgg cacgatggga acaaatactg tgggagcagg ttcttctgtc
      661 gccgaggcga acggagtctc ggctgagacc tgagtcgatg aagtggtaaa caagcctggg
      721 aggagactcg aagcgaaaac tccggtcaga acgacgcaaa gacaaagtcc cattaaccca
      781 attcctccta atactttggg aaagatggat ttttttgata caggcgttgg atgcggggtt
      841 gaggagaggt tcggtgcagg gacggcgaca cgactgggcg atgcatgtga ttcaatgatc
      901 gtgctgctta ttggctgttc aatcatggtt cccggagaac tacttttccc catagaacga
      961 atggcttggg acaattcgct agcggtttga tatctttcgg ctggtttctt ctccattgcc
     1021 ttgcggatga tcgtctcaaa ggattgtggc aagttggggt tgtggtctag caggctgggt
     1081 accggttcct ggatgtgagc aatggcaact cccataggtg tgtctgatac gaaaggcagt
     1141 ttcccgctga gtgcttcgaa caacaccacg cctagcgaat agatatcgct gcgaccgtct
     1201 atattctttt cacccattgc ctgttctggg ctcatgtaag ctggtgtgcc gaccatgttg
     1261 ccagtaccgg taaaggcgac actggtttct gccaattttg cgatgccaaa atcggaaagg
     1321 aaggcttcgc cttgcgagtc gaagaggatg ttgccgggct tgatatcacg atgcaccaca
     1381 ccggagcggt gggcgtaatc gagcgcggaa ccaatgcgtt gaaagagggg ctcgatctct
     1441 gtgagtgcta tgggaccatt tcccaaacgg tcggcaaggg tgccgccggg catgtagcgc
     1501 atgacgatgt aaggttggtc ttcatgttcc cccgaatcat agactggaac gatggcggcg
     1561 ttctcgagct tggcaaccac ttcaacttcg tgttggaagc gggcgcggaa ttgcggttcg
     1621 aaggtgaact gttttggcag aaccttgatc gccacttggc gtttgatgtt cgggtcatac
     1681 gccagataaa caattgccat gccgccctgt cccaattccc gtatgacctc ataccgacca
     1741 atattcatga ttgccttcca ataagagcca gtttgttatt gcttgaccat aattttcgat
     1801 aaactacgtc tgatttattt cagcgcatta tacattagat tatttcttgg cgatttttat
     1861 ttccaatcat tgatttcaaa acatgattta ataattttcg cct
//
LOCUS       DAOQFT010000512         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_42437_length_1899_cov_5.972343, whole genome shotgun sequence.
ACCESSION   DAOQFT010000512 DAOQFT010000000
VERSION     DAOQFT010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42437_length_1899_cov_5.972343"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLR93_11435"
     CDS             <1..484
                     /locus_tag="PLR93_11435"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNH27627.1"
                     /translation="KPYLDGFRAVFIKSNAVVAALQGGQIQAEFRSISPGERTQLVNA
                     LKDKKPLKANVGESIRIFFGVGGPNFTSSFHVIGEIFDRVYDQASLTSAPLTNVQTTL
                     VPPGGATMVEFNVEVPGNYILVDHALSRLQRGLAGVLIVEGDQNPEIYNGVPSAGSGH
                     "
     gene            540..1169
                     /locus_tag="PLR93_11440"
     CDS             540..1169
                     /locus_tag="PLR93_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27628.1"
                     /translation="MKNQTPLLLTVSLILLLVASCTPKATQDPNLLIQQAVNATLAAI
                     PTATQVSPPTPYPSPTPFSLAGLFCEYQFCIGHPIDVSFFDVSAQQNPASPSTYGQGI
                     LAALNGNLFIQVMWQLAPGTSDPKFLFDTMLNDSIDTATNTSSIFLVRDMNVFYDDIT
                     TLATPSLPYGGAGAWTCGDRVFAWKVYTPQSGTARGMFDEMLARFTCGQ"
     gene            1235..1483
                     /locus_tag="PLR93_11445"
     CDS             1235..1483
                     /locus_tag="PLR93_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27629.1"
                     /translation="MSAENDIIALRSRVAELERKLDFLYRRMGIEYVDDPGMVDSQII
                     SLLKKGNKLEAIKVYRELTNTDLADAKRAVEKIEASIL"
     gene            complement(1520..>1899)
                     /locus_tag="PLR93_11450"
     CDS             complement(1520..>1899)
                     /locus_tag="PLR93_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27630.1"
                     /translation="MSWLSKRVLHPHLPPLKVDTVILDAGMSLKEFGIPGEVIYTPGH
                     SMGHVSILLETGDALVGDMAMNDWYLRWTPGLPVLADNIEMVVDSWKKILPMGIKRVY
                     PAHGKDFDIEVMKKEIATFKLTK"
BASE COUNT          482 a          558 c          410 g          449 t
ORIGIN      
        1 caagccctat ctcgacggct tccgtgccgt cttcatcaaa tccaacgccg tggtcgcggc
       61 actgcaaggc ggccagatcc aggccgagtt ccgctcgatc tcgccaggcg aacgcaccca
      121 gctcgtcaac gccctcaaag acaagaagcc gctcaaagcc aatgtcggcg aaagcattcg
      181 catcttcttc ggtgtcggcg gaccgaactt cacatcctct ttccacgtca tcggtgaaat
      241 cttcgaccgc gtctacgatc aagcctctct caccagcgcg ccgctcacca acgtgcaaac
      301 caccctcgtc ccgcccggag gcgccaccat ggttgagttc aatgtcgaag tgcccggcaa
      361 ctacatcctt gtagaccacg ccttatccag attacaacgc ggtctcgccg gagtgctcat
      421 cgtcgaaggc gaccaaaacc cagaaattta taacggtgtc ccatctgcag gcagcggaca
      481 ctagaccaaa ccaaagcccc gtcgaaattg gacggggctt ttcctttata atttcgcgga
      541 tgaaaaacca aacgcctctt ctcttaacgg tgagcctgat tctgcttctt gttgcttcct
      601 gcacaccgaa agcgactcaa gacccaaacc tgctcatcca acaagcggtc aacgcaactc
      661 ttgccgccat tcccactgca acacaagtgt cgccacccac accttatccc tcgcccacac
      721 cttttagttt ggctggctta ttctgcgaat atcaattctg catcggtcat cccatcgatg
      781 tttccttttt cgatgtaagc gcccaacaaa acccagcatc tcctagcact tatgggcaag
      841 gcatattggc tgcgctcaac ggcaatctat tcattcaagt aatgtggcaa cttgcccctg
      901 gcacctcaga ccctaagttt ctgttcgata cgatgctaaa cgatagcatt gataccgcca
      961 caaacacctc cagcattttt ctagtccgcg atatgaacgt gttctacgat gacattacca
     1021 ctctggcaac gccgtcccta ccatatggcg gcgctggcgc ctggacctgc ggcgaccgtg
     1081 tcttcgcctg gaaggtatac acccctcaat caggcaccgc tcgtggcatg ttcgatgaaa
     1141 tgctagctcg cttcacctgc ggacagtaat aggaaatata ggtgaatgtc agcaagaacg
     1201 agcgggtgta atatcgtttt gaaggagata agctatgtct gctgaaaacg atattattgc
     1261 actgcgctca cgcgtcgctg aacttgaaag aaagctcgat tttctttata gaagaatggg
     1321 aattgaatat gttgatgacc ccggcatggt tgattcacag atcatttccc tgctcaaaaa
     1381 aggtaacaaa cttgaagcca tcaaggtcta ccgtgaattg accaataccg atttggcaga
     1441 tgccaaacga gcggtcgaaa aaatagaagc cagcatttta taaacaaaaa cgccagagaa
     1501 aatctctggc gtttttgttt cattttgtaa gcttgaacgt ggcgatctct ttcttcatca
     1561 cttcaatatc aaagtccttg ccatgtgcgg ggtagacccg tttgatgccc attggcaaga
     1621 tcttcttcca actatccaca accatctcga tattatccgc aagcacgggc agtccgggcg
     1681 tccaacgcag gtaccaatcg ttcatggcca tgtcccctac caaagcatca cccgtttcca
     1741 acaagatact tacatgtccc attgaatgcc ctggggtgta tatcacttcg ccgggaatgc
     1801 cgaactcttt cagtgacatg ccggcatcca aaatcaccgt atctactttg agcgggggaa
     1861 gatgcggatg tagtacccgc ttcgaaagcc acgacatgg
//
LOCUS       DAOQFT010000513         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_42859_length_1888_cov_7.617567, whole genome shotgun sequence.
ACCESSION   DAOQFT010000513 DAOQFT010000000
VERSION     DAOQFT010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42859_length_1888_cov_7.617567"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..579
                     /locus_tag="PLR93_11455"
     CDS             238..579
                     /locus_tag="PLR93_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27631.1"
                     /translation="MYEHHKQPLAKKSVFAKRLARNGVIGSLLLLFSLFIGMLGYHFL
                     EGLSWLDSLLNASMILGGMGPVNPLQTNAGKVFASLYALYSGVVLLAAVGILAAPIFH
                     RFLHRFHLAEE"
     gene            583..972
                     /locus_tag="PLR93_11460"
     CDS             583..972
                     /locus_tag="PLR93_11460"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HNH27632.1"
                     /translation="MTEYLPIHFYDESIEVLFEKPPVYEKAPHCPNGFIWNGQTYQVI
                     AALSEWTDFSRKGKMAKNMRPAHALTAATRGSLNVGRYYFRVEVNTAQIFDIYYDRAM
                     KSVDDRKGQWLMYRELEKTNGSIPAGQ"
     gene            947..1669
                     /locus_tag="PLR93_11465"
     CDS             947..1669
                     /locus_tag="PLR93_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH27633.1"
                     /translation="MDQYPLDNNPNLTPPLSTPKPASWSANDTWLGLALLLIVVAGYL
                     FALSRMEQNQSTNILYVATFEFLLLIPIAVIFLWRKVSWKELGFNRFNANQLALGCGL
                     LVGVYMLVIINNLVMISLGVTTQADVISELLGEINAPYLFAFTTVIVAPITEEMFFRG
                     FLFKGLREKYGWVNALMFSSMIFAIFHGQLATLLPTFLLGALFSYLYQRTDSVFPGMF
                     LHFTVNAMGALVLLFANQTGAL"
     gene            complement(1708..>1888)
                     /locus_tag="PLR93_11470"
     CDS             complement(1708..>1888)
                     /locus_tag="PLR93_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH27634.1"
                     /translation="NAAVARIFGISFSGFIAWMIWVFLHIYRIIGFRNRLIVMFNWAW
                     DYFFYDNQVRLITRE"
BASE COUNT          441 a          540 c          398 g          509 t
ORIGIN      
        1 gtgatcaccg gcaaaacacg cccctcaaaa agagactctg ccttggcagg ctccaccacg
       61 accaaatcat agcccaacac cagctcctta aactccgctt cgatcatatc ggctttgggg
      121 ctttgtgcct ctcgatacag tttaatcatt ctttctctcc ttcgtcattc agacgatttt
      181 actcagtaat ttcttccaat ccccacatca actataattc tcatatcaaa ggttaacatg
      241 tacgagcatc ataaacaacc acttgcaaaa aaatctgtct ttgccaaacg ccttgcccga
      301 aacggcgtga ttggatctct gttactgctc ttctctttat tcatcggcat gctcggctat
      361 cacttcctcg aaggtctctc ctggttggac tccctgctca atgcctcgat gatcctcggc
      421 ggcatgggtc cggtcaaccc gctgcagacc aatgccggga aagttttcgc ctcgttatac
      481 gctctatatt ctggcgtcgt acttctggca gccgttggca ttcttgcagc gccgatcttt
      541 catcgcttct tgcatcgctt ccacctcgca gaagagtaaa ctatgaccga atatctgccc
      601 atccactttt atgatgaaag catcgaagtc ctcttcgaga agccgcctgt gtatgagaaa
      661 gcgccacact gccccaatgg cttcatctgg aacgggcaga cttatcaagt cattgccgcc
      721 ctctccgaat ggaccgactt ctcgcgcaaa ggcaaaatgg cgaagaacat gcgccccgcc
      781 catgccctaa ccgccgccac ccgcggctcg ttgaatgtcg gtcgctacta ctttcgcgtg
      841 gaagtgaaca ccgctcaaat cttcgacatc tactacgacc gcgccatgaa aagcgtagac
      901 gaccgtaaag gtcaatggct gatgtataga gaattggaaa aaacaaatgg atcaataccc
      961 gctggacaat aacccgaacc tcacgccgcc tctttccacg cccaagcctg cctcttggtc
     1021 tgcaaacgac acctggctcg ggctggctct tctgctcatc gtcgttgccg ggtatttatt
     1081 tgcactttct cgcatggaac aaaatcaaag caccaacatc ttgtatgttg ccaccttcga
     1141 attcctttta ttgattccca ttgccgttat cttcttatgg cgcaaagtct catggaaaga
     1201 gctgggcttc aaccgtttca atgccaatca attggcattg ggttgcgggt tgttagttgg
     1261 cgtgtatatg ctggtcatca tcaacaatct cgtgatgatc tcactcggcg tcaccaccca
     1321 agccgatgtc atctccgagc ttcttggcga aattaacgcc ccgtatctct tcgcctttac
     1381 caccgtcatt gtcgcgccta tcaccgaaga gatgttcttc cgtggctttc tcttcaaagg
     1441 cttgcgcgaa aaatatggct gggtcaatgc cttgatgttc agctcgatga tctttgccat
     1501 ttttcatggg caacttgcta ctttattgcc tacttttctg ctcggcgcct tgttctctta
     1561 cctgtaccag cgtacagact cagtcttccc gggcatgttt ctacatttca cggtcaacgc
     1621 catgggggca ttggtcttgc tcttcgcaaa tcaaaccggt gctttataag aatacatcct
     1681 cagagaaaat ctctgaggat gtattcttta ttcccgcgtg attagtcgta cttgattatc
     1741 gtagaagaaa taatcccacg cccagttgaa catcacaatc agacggttgc gaaacccgat
     1801 gatgcgatag atgtgcagga agacccagat catccaggcg atgaatccgc tgaaggagat
     1861 gccaaagatg cgggcgacgg cggcgttc
//
LOCUS       DAOQFT010000514         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_42897_length_1887_cov_4.296397, whole genome shotgun sequence.
ACCESSION   DAOQFT010000514 DAOQFT010000000
VERSION     DAOQFT010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42897_length_1887_cov_4.296397"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLR93_11475"
     CDS             <1..258
                     /locus_tag="PLR93_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27635.1"
                     /translation="YSSNTFLNYQRAHLDYGKIWTHGNRLVWIDSTKESLPKPLPAWS
                     EWFISYKGDNPLFFAFVFIIIITIWHFTLKHINRKKLEKMR"
     gene            255..653
                     /locus_tag="PLR93_11480"
     CDS             255..653
                     /locus_tag="PLR93_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27636.1"
                     /translation="MIFSPTIHVHITREHFVFQYEGQELHLEACIHYLLEGNEVTPVA
                     IGDRVEIPNAHFADLFKPKEPYTDHFYLLQLLLEYGFGRMLGKRLLPSKPDVIFHGVE
                     QLYEILSGYQFGIFEKAALQAGVRSVKFEQ"
     gene            650..1411
                     /locus_tag="PLR93_11485"
     CDS             650..1411
                     /locus_tag="PLR93_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27637.1"
                     /translation="MKRDLYFSKPLINAAGSLGFAPDLRGFETLGGLKFGAFVTNPIS
                     RRPRLPTSHPALVEFPGGFLLHTGLPNPGFHAVLKKYAAKWNRADLPIIVHLMADRPE
                     ETQSMVRELEGVENVMAVQLGFAPLLSDDIILMNLEMCLGEVPLIFALPHEQVLTLGP
                     RLMEEGASAVSLASPRGAVYDKAGNLITGRMYGQSLFPRALDVVRSAAMIGLPMIGSG
                     GVWSATDVESMLKAGAMAVETDAQLWVPKEAGFIS"
     gene            complement(1487..1696)
                     /locus_tag="PLR93_11490"
     CDS             complement(1487..1696)
                     /locus_tag="PLR93_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNH27638.1"
                     /translation="MAERIVGTVKWFNTQKGYGFLARDGGPDVFVHFSAIRGDGFKNL
                     AEGQRVEFTIEKGPKGLQAADVVIL"
BASE COUNT          468 a          453 c          472 g          494 t
ORIGIN      
        1 tattcatcca acacattctt aaattatcaa agggctcatt tagattatgg gaaaatttgg
       61 acacacggaa atcgtttggt ctggatagat agtaccaagg aaagcctgcc aaaacctctg
      121 ccagcctggt cggaatggtt tattagttat aagggggaca atcctttatt ttttgcattt
      181 gtttttatca taataattac aatctggcat tttactttga agcacattaa tcgaaaaaaa
      241 ctggagaaaa tgagatgatt ttttcaccaa caatccatgt tcatatcaca cgcgaacatt
      301 ttgtcttcca atacgaaggt caggaacttc atctggaagc atgcatccat tatcttttgg
      361 agggcaatga ggttaccccc gttgccattg gcgaccgggt ggaaattcca aacgcccatt
      421 tcgcagacct tttcaaaccc aaagagccct ataccgatca cttctattta ctgcaattgc
      481 tgcttgaata cggttttgga agaatgcttg gaaaaagact tctaccgtcc aagccagatg
      541 ttatctttca tggtgtggaa caactgtatg agatcctaag cggatatcaa tttggcatct
      601 tcgaaaaggc tgccttgcag gctggcgtcc gcagtgttaa attcgaacaa tgaagcgcga
      661 cctgtatttt agcaaacctc tcatcaatgc agcggggtcg ttgggattcg ctccagacct
      721 ccgaggtttt gaaaccctcg gaggtctgaa attcggcgcg tttgtgacca acccgatctc
      781 gcgccgccca cgcctgccga cttcccatcc ggctttggtc gaattccccg gcggattcct
      841 ccttcacacc ggcctgccga atcccggatt ccatgcggta ctgaaaaaat atgccgccaa
      901 gtggaatcgc gccgacctgc ccatcatcgt ccatttgatg gcagaccggc cggaggaaac
      961 tcaatccatg gtgcgcgaac tggaaggcgt ggagaatgtg atggcggttc aacttgggtt
     1021 tgcgccgctt ctatcagacg acatcattct gatgaatttg gaaatgtgcc tgggtgaagt
     1081 tccgctgatt tttgcgctcc cccatgagca ggtgctgaca ttaggtccgc gtttaatgga
     1141 ggaaggcgca agtgcggtta gtttggcgtc tccgcgcggg gctgtgtatg acaaggccgg
     1201 gaatttgatc accggaagaa tgtatgggca atcgttattt ccgcgcgcat tggatgtcgt
     1261 tcgttcggcg gcgatgatcg gcctgccgat gatcggctcc ggcggcgtct ggtcggcgac
     1321 ggatgtggag tcaatgctca aggcgggggc aatggccgtg gaaacagatg cccagctttg
     1381 ggtcccgaaa gaggcggggt ttatatcctg aagtagaact tcagcagtcc agtcaaaata
     1441 aaaaagagtc ccgtcttgcg acgagactct ttttggttga ttcggattag agaattacta
     1501 catccgcggc ttgcaggccc ttgggacctt tttcgatggt gaattcaacc cgttggcctt
     1561 cagcaagatt cttgaagcca tcaccacgaa tggcggagaa gtggacaaag acatctgggc
     1621 cgccatcacg agcaagaaag ccgtagccct tctgggtgtt gaaccacttt accgtaccga
     1681 cgatacgttc agccatgttt gcctcctaag caaataaaaa aaatttaggt gacgcaggtt
     1741 gtctttcatc gggcggtaaa ataaacggct caccgcggta gtatgagccg ttgcctttct
     1801 ccttccacaa cgaaaaccta cttgcatctt cgcgcctaat ttatcatgaa attctaatct
     1861 tggcaattat ttggcgggct cgagcaa
//
LOCUS       DAOQFT010000515         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_43139_length_1881_cov_5.997809, whole genome shotgun sequence.
ACCESSION   DAOQFT010000515 DAOQFT010000000
VERSION     DAOQFT010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43139_length_1881_cov_5.997809"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..298)
                     /locus_tag="PLR93_11495"
     CDS             complement(95..298)
                     /locus_tag="PLR93_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27639.1"
                     /translation="MISKLLDDLSNYLAHRKGLIPIIGLLLIILNLILQFIFPGSLIV
                     TTNLFLHIGLIIAIFGLMLAWAL"
     gene            complement(295..1665)
                     /gene="hflX"
                     /locus_tag="PLR93_11500"
     CDS             complement(295..1665)
                     /gene="hflX"
                     /locus_tag="PLR93_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNH27640.1"
                     /translation="MAKKLPQPTTPPRERAFLVGADLHDNHSILSLEDSLAELALLAD
                     TAGVDVVGELTQKLNRPHVETYIGPGKVEELKALAEETLCEVVIFDDELLPRHQRELE
                     KALGKNIRVIDRTALILDIFAQHAHTKEGMLQVELAQYEYNLPRLTRAWTHLERQAGG
                     GGGRSGSTGGVGLRGPGETQLEVDRRAIRRRITHIKKELEKVEEHRTRYRAQRRRTRI
                     PTVALVGYTNAGKSTLLNRLAKADVYVADQLFATLDPTTRRVQVSGENTVLFTDTVGF
                     IQKLPTALVVAFHATLEEIAEADLLLHVVDISHPNALGQYQAVLETLKEIDADHIPMI
                     TALNKMDQLRDPQNAERVLENFPKSVAISALKGTGIDNLLMLVQEELFEAFEPIGVRL
                     PYKEGSLISLFHELGQVERVEHERGGVIIHGSIPGRLLAQFAPWKIKPNGNTSHTLEE
                     MDLEEE"
     gene            complement(1625..>1881)
                     /locus_tag="PLR93_11505"
     CDS             complement(1625..>1881)
                     /locus_tag="PLR93_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27641.1"
                     /translation="KGGGPGLGLSITRGIIEAHGGTIWVESTGYDEVQLPGSIFHILL
                     PTRTEPNNPVMLKFFGNNETKMTETESSGKETPPTDNPSA"
BASE COUNT          460 a          431 c          497 g          493 t
ORIGIN      
        1 aagtaatgaa ctcttcaatt cgatattggt tgagcagggc tttgatcgta acggaagagg
       61 ttgacattga agtgcgtcgc gatcaggcgt aggtttagag cgcccatgcc aacatcaaac
      121 cgaagatggc aataatcaac ccgatatgca ggaaaaggtt tgtggtgacg attagagaac
      181 cgggaaaaat aaattgtaag atcagattga ggatgatcaa aagcaaccca ataatgggaa
      241 ttaacccttt gcgatgggca agataattgg aaagatcatc gagtaatttt gagatcattc
      301 ttcctccaaa tccatttctt ctaaagtgtg tgaggtattg ccattgggct ttatcttcca
      361 cggtgcgaat tgtgctaaaa gcctgccggg aatgctgcca tgaataatga caccaccgcg
      421 ttcatgctct acgcgttcca cttgacctaa ctcgtggaat aaagaaatga gagaaccttc
      481 tttatagggc aacctcacac cgatcggctc aaaagcttca aataattctt cctgtaccag
      541 catgagtaag ttatctatgc cggtgccttt cagtgcggag atagccacag acttcggaaa
      601 gttttccaaa acacgttcag cgttttgtgg gtctctcaac tggtccattt tgttcaatgc
      661 ggtgatcatg gggatatgat cggcatcgat ctctttcaag gtttcgagca cagcttgata
      721 ttgcccaagg gcgttgggat gagaaatatc cacaacgtgt aagagcagat ctgcttcggc
      781 gatttcttcg agggtggcgt gaaaggcaac caccaacgcg gtaggcagtt tttgaatgaa
      841 gcccactgtg tcggtgaaca atacggtatt ttcaccagag acttgcacac ggcgtgttgt
      901 ggggtcgagg gtggcgaata attgatctgc cacgtatacg tctgctttag caagacgatt
      961 caacagtgtg gatttgcctg cgttggtgta cccaaccaag gcgacggtgg ggatgcgggt
     1021 gcgtcgacgt tgagcgcgat agcgtgtgcg atgctcttcc accttttcca attctttttt
     1081 gatatgggtg atgcgtctac gaatagcacg gcgatctacc tcgagctgcg tttcaccagg
     1141 accgcgcaaa cccacgccgc ctgtcgagcc ggaacgtccg ccgccaccgc ctgcctgacg
     1201 ttcaagatgc gtccatgcgc gggtgaggcg tggtaagttg tattcgtatt gagcaagctc
     1261 cacttgcaac atgccttctt ttgtgtgcgc atgttgtgca aagatatcga ggatcagagc
     1321 cgttcggtca atgacgcgga tgtttttgcc aagcgccttt tccaattcgc gttggtgacg
     1381 tggcagaagt tcatcatcga aaataaccac ttcacacaaa gtttcttctg cgagcgcttt
     1441 gagttcttcc accttgccgg gaccgatata cgtctcaacg tgcggacggt tgagtttttg
     1501 agtcagttca ccgaccacgt ctacaccggc agtatctgcc agcaaggcaa gttcagcaag
     1561 agagtcttcg agagaaagga tggaatgatt atcgtgcaga tccgcgccca ctaaaaaggc
     1621 gcgttcacgc ggaggggttg tcggttgggg gagtttcttt gccacttgat tccgtttctg
     1681 tcattttcgt ttcgttatta ccaaaaaact taagcatcac cgggttgttc ggttcggtgc
     1741 gggtcgggag caggatatga aaaatggaac ccggtaattg tacttcgtcg tatcctgtcg
     1801 attcaaccca gatggtgccg ccatgcgctt caataatgcc gcgggtgatg gatagaccaa
     1861 gaccgggtcc tccgcctttg a
//
LOCUS       DAOQFT010000516         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_43170_length_1880_cov_6.329863, whole genome shotgun sequence.
ACCESSION   DAOQFT010000516 DAOQFT010000000
VERSION     DAOQFT010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43170_length_1880_cov_6.329863"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLR93_11510"
     CDS             complement(<1..766)
                     /locus_tag="PLR93_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH27642.1"
                     /translation="MYDKKKLDEIQQSLSKWEETSLKKALNQLPERADEFITTSSEPI
                     NRLYTPLDTADLDYNDSLGNPGEYPYTRGVHPTLHRSKLWTMRMFAGFGTAEETNGRF
                     KYLLEQGQTGLSIAFDLATLMGYDTDQPEALGEFGKCGVAVSSLKDMEILLDGIPLDK
                     VSSSMTINSPAAIIWAMYLVAAEKQGVRLDQLRGTLQNDILKEFIAQKEFIFPPEPSM
                     RLVVDTMEYGSKHVPQWNTISISGYHIREAGSTAAQE"
     gene            922..1449
                     /locus_tag="PLR93_11515"
     CDS             922..1449
                     /locus_tag="PLR93_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HNH27643.1"
                     /translation="MSRTDIDVDPCDHITADAIGKPGQRVFYLQAFQDNRTITIIIEK
                     AQLISLAIGIEQFLAQISQQYPDLEEASGDYIEEVMRINPPVDPLFRSGEIGLGYDKD
                     RDRVVVFAKELLTEDADPESASQVRFWATRSQLRRLARWGQEVSSRGRPVCPQCGQPM
                     EPEGHFCPKKNGHLH"
     gene            1464..>1880
                     /locus_tag="PLR93_11520"
     CDS             1464..>1880
                     /locus_tag="PLR93_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27644.1"
                     /translation="MNSKIIAALTHGDYTLRGQFMLGSNYTFLVDVQHEGETFKAVYK
                     PCKGEQPLWDFPDNTLALREVAAYQLSEALGLHIVPVTLFREDGPHGPGSLQQYIEYD
                     VEYHYFNFTVEDKQKLRPVVFFDFIANNADRKGSHIF"
BASE COUNT          446 a          488 c          458 g          488 t
ORIGIN      
        1 gctcttgcgc agccgtcgaa cccgcttcac ggatgtgata gcccgaaatg gaaatggtat
       61 tccactgcgg tacgtgtttc gagccgtatt ccatcgtatc caccacgagg cgcatggacg
      121 gttccggcgg gaagatgaat tccttctgtg caataaattc tttgaggatg tcgttctgaa
      181 gcgtgccgcg caattgatct aaacgcacgc cttgcttttc agcagcgacg agatacatcg
      241 cccagatgat ggcagcgggc gagttgatgg tcatgctcga agaaacttta tcgagcggaa
      301 tgccatccag caaaatttcc atatctttga gtgaagaaac agctacgcca catttgccga
      361 attcacccag tgcttcgggt tggtcagtat cgtagcccat cagcgtggca aggtcgaagg
      421 caatgctcaa gccggtctga ccctgctcaa gtaagtattt gaagcgcccg ttcgtctctt
      481 ctgcagtacc aaagcctgca aacatacgca tggtccacag tttggagcga tgcaacgttg
      541 ggtgaactcc acgtgtgtat ggatattcac ctgggttgcc gagcgagtcg ttgtaatcta
      601 aatcggcggt gtcgagcggt gtataaagtc tgttgatcgg ttcggaggaa gtagtaataa
      661 attcgtcagc acgttcaggg agttgattta aagctttttt gagagaagtc tcttcccact
      721 tggaaagcga ttgttgtatt tcatcgagct tttttttatc gtacatggca tctcctttgg
      781 cgagttgatg agattatacc gccccctcct cctactatca cgggcatctc ttcccatatg
      841 taatgaatta actttcctca cattttaggg aggatgggtg gggatggcta ttctggtata
      901 ctctcaaaaa aggtaatctt tatgtctcgc actgatattg acgtcgaccc ctgtgatcac
      961 atcaccgctg acgcgattgg caaacctggt caacgcgttt tttatctgca agcctttcag
     1021 gataaccgca ccattaccat tatcatcgaa aaggcgcaat tgatctcgct ggccattggc
     1081 atcgaacaat tcctggcgca gatcagccag caataccccg acttggaaga agcttccggc
     1141 gattacatcg aagaggtcat gcgcatcaac ccgccggttg acccgctctt tcgttccggt
     1201 gaaattggcc tcggctacga taaagatcgt gaccgtgtag tcgtttttgc caaggaactg
     1261 cttaccgaag atgccgaccc agagtctgcc tcacaggtcc gtttttgggc cacacgcagt
     1321 caactgcgcc gccttgcccg ttggggccag gaagtctctt cccgcggacg tcccgtctgc
     1381 ccgcagtgcg gccagcccat ggagccggaa ggacatttct gcccgaagaa gaacggacat
     1441 ttgcattaac gtctaatcgt cccatgaatt ctaaaattat agctgcgctc acacatggcg
     1501 attacaccct tcgcggtcaa ttcatgcttg gctccaatta cacatttctg gtggacgtcc
     1561 agcatgaagg cgaaacattc aaagctgttt ataagccttg caaaggggaa caacccctat
     1621 gggactttcc tgataatact cttgccttac gcgaagttgc cgcctatcaa ttgagtgaag
     1681 cgctgggcct gcacatcgtt cccgtgaccc tgtttcgtga agacggcccg catggccccg
     1741 gctcgttaca gcaatacatt gaatacgatg tcgaatatca ctacttcaac ttcaccgtgg
     1801 aagataagca aaaacttcgt ccggttgtat tcttcgactt catagccaac aatgccgatc
     1861 gcaaggggag tcacatcttt
//
LOCUS       DAOQFT010000517         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_43334_length_1876_cov_5.975288, whole genome shotgun sequence.
ACCESSION   DAOQFT010000517 DAOQFT010000000
VERSION     DAOQFT010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43334_length_1876_cov_5.975288"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLR93_11525"
     CDS             complement(<1..735)
                     /locus_tag="PLR93_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27645.1"
                     /translation="MKPYFKYFIFFILLGIFLAACAGADFQQVDKEYVLTTDLRNGNL
                     VFLGVSDEINGQENPTLSAKAGERITITLINGGEGEHSITLPELNASSEIVKEKGEET
                     SITINVPNVHGEIGYYDGVGNHADLGMRGKLVVTETGAAMPAMNTTTTSNGDPAVLAA
                     FQKGACGSCHTISGIPGAVGVVGPKLDDISVMSEEMIKDTSYSGHATNAEEYIRESIT
                     DPNLFLAPECPTGACAPNVMPATLTQT"
     gene            complement(732..962)
                     /locus_tag="PLR93_11530"
     CDS             complement(732..962)
                     /locus_tag="PLR93_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27646.1"
                     /translation="MNDELEISEIHVESILKAQPNAARFFLNWKLGCVGCGFARFCTL
                     ADVIHTYKLDEKKFLEEAKQLVVHHTLMRSSE"
     gene            complement(1042..1740)
                     /locus_tag="PLR93_11535"
     CDS             complement(1042..1740)
                     /locus_tag="PLR93_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH27647.1"
                     /translation="MKISPQDLKQVVVFQHATDEDLELILKNSITRSIEEGSFFFMQG
                     DEAHYLYILTSGQVKLMQSNPNGQQVNLRTIYPWQMFGALGAVRTDGAAYPASAQTLE
                     DSTALATPSLFLRSMLETRPYLSFDLMNLMTSYIQEMQARYRELATERVEQRVANALV
                     RLAGQSGTRSDKEAGIELSFSRQDVAEMTGTTLYTVSRLLSDWERKGIIKTGREKILV
                     LNPHELVRIADGQV"
BASE COUNT          432 a          468 c          485 g          491 t
ORIGIN      
        1 ggtctgagtt agagtggcgg gcatcacgtt tggagcacaa gcaccagtgg ggcactcagg
       61 cgcgaggaag agattcgggt ctgtaattga ttcgcggatg tattcttcag cattggtcgc
      121 atgacctgaa tagcttgtat ctttgatcat ctcttcagac atgacgctga tgtcatcgag
      181 ctttggtccg acaacgccga cggcacctgg aattccagaa atggtatggc aggaaccgca
      241 ggctcccttt tggaaggcag cgagcacggc agggtcaccg ttgctggtag ttgtggtatt
      301 catggctggc atcgctgcgc cggtttcggt cacaactaat ttgccgcgca tgcccaaatc
      361 tgcatggttg ccgacaccgt cgtaatagcc aatctcacca tgtacattag gcacgttgat
      421 ggtaatggat gtttcttcgc ctttttcttt cacgatctca gaactggcgt ttagttctgg
      481 aagagtaatg ctatgctcgc cttcaccgcc gttgatgagg gtgatggtga ttcgttcccc
      541 cgctttagcg ctgagcgtag gattctcctg cccgttgatc tcgtcactta cgccgagaaa
      601 gaccaaattg ccgttgcgta agtcggtcgt caagacatat tctttgtcca cctgttgaaa
      661 gtctgcgcct gcacaggcag caagaaaaat gccaagcagg atgaaaaata taaaatactt
      721 gaaatagggt ttcattctga actcctcatt aaggtatggt gaacgacaag ttgtttggct
      781 tcttccagaa attttttttc gtcaagctta taagtgtgaa tgacgtctgc caatgtgcag
      841 aagcgagcaa aaccgcagcc aacacatccc agtttccagt tcaagaaaaa acgggcggca
      901 ttgggctgag cttttagaat gctctccaca tggatctcag aaatttccag ttcatcgttc
      961 atagtggcta cagaatatcg cattccccca aacccttctt tgcgctggcg caaataaaaa
     1021 gagcgcaacc ttgctgcgcc cttacacttg tccgtctgct attcttacca attcatgcgg
     1081 attgagaacc agtatctttt cccgcccggt cttgatgatg cctttccgct cccaatcact
     1141 gagcagacga ctgaccgtgt acagtgtggt gccggtcatc tctgccacat cttgccggga
     1201 gaaggatagt tcgatccccg cctctttatc ggaccgagtg cctgattgcc cagccaagcg
     1261 gaccagcgcg ttagccactc tctgctcgac ccgctcggta gcaagttcgc ggtaacgtgc
     1321 ctgcatctct tggatgtagc tggtcattag gttcattaag tcaaaggaaa ggtaggggcg
     1381 agtttcgagc atggagcgca gaaacaggct cggggtcgca agagccgtgc tgtcctccaa
     1441 ggtttgagcg gaagcagggt aggcggctcc atccgtgcgg acggctccca atgcgccgaa
     1501 catctgccac gggtagatcg tccgcaggtt cacttgttgc ccgttgggat ttgactgcat
     1561 gagcttgacc tgtccgctgg tgaggatgta cagataatgg gcttcatcac cctgcataaa
     1621 gaagaaactg ccttcttcta tggagcgggt aatgctattt tttagaataa gttcaaggtc
     1681 ttcatcggtt gcgtgttgaa aaacaacgac ctgtttgaga tcttgggggg aaattttcat
     1741 aaagtcattt tacaccccaa tccgatgaca tttatcgact tgacagaggt gtgggggatg
     1801 acttatagtt tggttatccc tacccccccg ctgatgccgt acatcacgga agaaatctgg
     1861 caacgcatcg cgccgc
//
LOCUS       DAOQFT010000518         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_43710_length_1867_cov_7.644040, whole genome shotgun sequence.
ACCESSION   DAOQFT010000518 DAOQFT010000000
VERSION     DAOQFT010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43710_length_1867_cov_7.644040"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..957)
                     /locus_tag="PLR93_11540"
     CDS             complement(22..957)
                     /locus_tag="PLR93_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH27648.1"
                     /translation="MIETNDLSKQFHDFMAVDGVNLSIGAGQILALLGQNGAGKTTTV
                     RMLTALLQPSRGSARVAGYDVTKNGHDVRASVGVLTEHHGLYMRMTGSEYMDFFGQVY
                     NLDPSLRKSRTENLLEYFGLAEASKRRIGEYSKGMRQKLALARAMMHEPSVLLLDEPT
                     SAMDPESARLVRDEIARLKSTQRTIMLCSHNLTEVELLADKIAIIYRGKILIQGSLDE
                     LKSGVLGAPEYVIKFASAWDASRLEMPKGMELLEKTATSLKVRVEAPAESNPELIQRL
                     TSLSAPVVAFQEETRTLEQVYLKVMATAKGKEYVQ"
     gene            complement(1035..1835)
                     /gene="lgt"
                     /locus_tag="PLR93_11545"
     CDS             complement(1035..1835)
                     /gene="lgt"
                     /locus_tag="PLR93_11545"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH27649.1"
                     /translation="MTDGLNLGFLLIRWNGLLIALGIAVGALVAAIEAKRRFQDAELI
                     YYLFVPVTVWSWIGARLWHVFTPPLSSVQLGLTTSHYLSHPLDILSVWTGGFGIPGAI
                     LGGAFALWLASRKYELSFWEVADVLALGMVLAQAIGRIGNYFNQELYGLPTNLPWSIF
                     IQPDHRLLSYEQVEFYHPLFAYEAIFVFLGFVLLFWLSRSKFADTFFAGDIYLGSLAF
                     YSLVRFLLENLRLDVVLVNGVNANQLFFAILFIVSISILGHRRLAPVL"
BASE COUNT          545 a          467 c          422 g          433 t
ORIGIN      
        1 acaagccaaa caggtcttaa tttattgaac atattctttt ccctttgcag ttgccatcac
       61 cttcaagtag acctgttcca gtgtgcgcgt ttcttcctga aaggcaacta caggcgcgga
      121 caaggaagtg agccgctgaa tcaattctgg atttgactct gctggcgctt ccacccggac
      181 tttcaaactg gtcgctgtct tctcgagtaa ttccatccct ttgggcattt ccaacctact
      241 cgcatcccaa gcagatgcaa atttgatgac atattcagga gcccccaaaa cgcctgactt
      301 caactcatct aatgagcctt gaatcaaaat ctttccacgg taaataatcg cgatcttatc
      361 tgcaagaagt tccacttcgg tgaggttgtg agagcacaac atgatggtcc tttgcgtaga
      421 cttgagcctc gcaatttcat ctctcaccaa ccgcgcggac tcggggtcca ttgcagaggt
      481 gggctcatcc aaaagtaaga cgctgggttc gtgcatcatt gcgcgagcaa gcgctaattt
      541 ttggcgcatc cccttggaat attccccgat acggcgtttg gaggcttctg ccaagccaaa
      601 gtattcaagc aggttttcgg tacgtgattt acgaagggaa gggtcgaggt tatagacttg
      661 cccaaaaaaa tccatgtact ctgaaccggt catacgcatg tacaaaccgt gatgttcagt
      721 gagcacgccc accgaagcac ggacgtcatg cccgtttttt gtcacatcgt agccagctac
      781 acgtgcagag ccgcggcttg gctgcaataa ggcagtcaac atacgcacgg tcgttgtttt
      841 gcctgcgcca ttttgcccca acaaggcaag gatctgcccg gcaccaatgg agagatttac
      901 tccatcgaca gccataaaat catggaattg tttactaaga tcgttggtct caatcatgag
      961 gtatcctttt ctactaagaa agaggctacg ctcattttat tacccatccc ccacggcttt
     1021 ccttctcaaa tcccttacaa aactggtgca agccttctat gcccaaggat gcttatagag
     1081 acaatgaaaa gaatagcaaa aaatagttga tttgcattga cgccattgac caataccacg
     1141 tcgaggcgaa ggttctcaag cagaaaacga actaaggaat agaatgctaa agaaccgaga
     1201 tagatatcac cagcaaagaa ggtatccgca aatttgctgc gagaaagcca aaataacaag
     1261 acaaagccca gaaacacaaa aatggcttca taagcaaata agggatggta aaactcgact
     1321 tgttcgtaac tgagcaagcg atggtctggc tgaataaaaa tcgaccaagg caaattcgtt
     1381 ggcaagccgt agagttcttg attgaaataa ttgccaatac gccctatggc ttgcgccaat
     1441 accatgccca gagcaaggac atctgccacc tcccaaaaag agagctcata tttacgagat
     1501 gccaaccaca aagcaaaagc tccgccaagg atggcgcccg gaataccaaa gccgcctgtc
     1561 catacagaga gaatgtctaa gggatgagag agatagtgac tggtggtaag accaagctga
     1621 acggaggaaa gaggcggagt gaatacatgc caaagcctgg caccaatcca tgaccacacc
     1681 gtcacaggga cgaagaggta gtagataagc tccgcgtctt gaaagcggcg tttggcttca
     1741 atggctgcga ccaaggcacc aaccgcaatg cccaaagcga tcaataaccc gttccagcgg
     1801 atcagtaaaa aaccgaggtt taagccgtct gtcacttggc ttccttggat tcttttgatt
     1861 tcttaat
//
LOCUS       DAOQFT010000519         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_43711_length_1867_cov_6.721854, whole genome shotgun sequence.
ACCESSION   DAOQFT010000519 DAOQFT010000000
VERSION     DAOQFT010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43711_length_1867_cov_6.721854"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1867
                     /gene="mutS"
                     /locus_tag="PLR93_11550"
     CDS             <1..>1867
                     /gene="mutS"
                     /locus_tag="PLR93_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNH27650.1"
                     /translation="MEASASSSGTVASVAYTDVTTGEFAVTELPLEALRAELTRLHPA
                     EILYPDNQKLPDGIIGHTTHVPAWKFEPGKCNEVLLTQFKASTLDGFGLKANSLSVRA
                     AGAIIQYLKETQPDALHLLNSLRSYSLNEFMTLDASTRRNLELDETLRGERKGSLLGT
                     LDATITAMGKRMIHQWVSQPLLNVERINKRQNGVEYFVQNGMVRAELREALRPIADLE
                     RLINRVIAGHAQPRDLVAMRSTFANLPNILNALKEKGNKKEAQLPTIDLLGEQLSILQ
                     NAIDEDPPATLQNTGVIRPGYSTELDGVIDASKHAREWIANLESVEREKTGIKTLKVG
                     YNKVFGYYIEISRGAADKAPEHYIRKQTLVNAERFITPEMKEYETLVLNAEERIKEIE
                     TRLFKEVCGDLSKTANKILATARSIAELDVLSALGEVAALNGYTKPQVHEGSELEITE
                     GRHPVVEQLLKSDRYIPNDVIFEKGEIVRVITGPNMSGKSTYLRQTALIVLMAQMGSF
                     VPAGHVKIGLVDRIFTRIGAQDEIHAGQSTFMVEMVETANILHHATNRSLLILDEIGR
                     GTSTYDGLSIAWGIIEYVHNHPQLRAKTLFATHYHELTQLAELLPGVRNYNVAVSE"
BASE COUNT          527 a          472 c          426 g          442 t
ORIGIN      
        1 atggaagctt ccgcctcatc ctccgggaca gttgcctctg ttgcttatac cgacgtcacc
       61 actggagaat tcgccgtcac cgaacttccg ctcgaagcct tacgcgcaga gttgacacgc
      121 ctgcaccccg ctgaaattct ttatcctgac aatcaaaagc ttcctgatgg aatcatcggt
      181 cataccacgc acgtccctgc ttggaagttc gaaccgggta aatgcaatga agtacttctg
      241 actcaattca aagcctctac cttagatggg tttggactga aagccaatag cctctccgtc
      301 cgcgctgctg gagcaatcat tcagtacctg aaagaaacgc agccagacgc tctccatctg
      361 ctgaactcgc tccgctctta tagcctaaac gagttcatga ccttggatgc atccacacgc
      421 cgaaaccttg aactcgatga aaccctgaga ggcgaacgca aaggttccct gctcggaaca
      481 ctcgatgcaa ccatcacagc catgggcaag cgcatgatcc atcagtgggt cagccagcct
      541 ttactaaatg tagaacgaat taacaaacgc caaaatggcg tggaatattt tgtgcagaat
      601 ggtatggtcc gcgctgaatt gcgcgaggca ttacgcccca tcgctgattt agagcgtctc
      661 atcaatcgtg tgattgctgg gcacgcacag ccgcgcgacc ttgtggcaat gaggtcaacg
      721 tttgcgaatt taccaaatat cctcaatgcc ctaaaagaaa agggaaacaa gaaagaagcg
      781 caattaccca caattgattt gctgggcgaa caattatcca ttttacaaaa tgccattgat
      841 gaagacccgc cagccacact acaaaacacc ggtgtgattc gtcccggcta ctcaactgaa
      901 cttgacggtg ttattgacgc ctccaaacac gcccgtgaat ggattgccaa cctagagtcc
      961 gtcgaacgcg aaaaaacagg tatcaagacc ctcaaagttg gctacaacaa agtctttggc
     1021 tattacatcg aaatttcgcg tggcgccgcc gataaagccc cagaacatta catccgcaag
     1081 cagacattgg tcaatgccga gcgtttcatc acacctgaga tgaaagaata tgaaacgctg
     1141 gtcttgaatg ccgaagaacg catcaaagaa attgaaactc gtttattcaa agaagtttgc
     1201 ggcgatctct caaaaactgc aaataaaatt cttgccaccg cccgctctat agccgagctg
     1261 gatgtactgt ctgctctagg cgaagtcgca gccttgaatg ggtataccaa gccccaagtc
     1321 catgaaggaa gcgagttgga aatcacagag ggcagacacc cagttgttga gcaattatta
     1381 aagtctgacc gttacattcc caatgacgtc atctttgaaa aaggcgagat tgtgcgggtg
     1441 atcacgggac cgaatatgag tggtaagtct acatatttac gtcagaccgc tctgattgtg
     1501 ttgatggcgc aaatgggttc gtttgttccg gcagggcatg taaaaatcgg tttagtagac
     1561 cgtatcttca cccgcattgg tgcacaagat gaaattcacg ctgggcaatc aacctttatg
     1621 gttgaaatgg ttgaaaccgc aaatattttg catcatgcca caaatcgcag tttgctgatt
     1681 ttagatgaaa tcgggcgcgg cacttcgact tatgatggtc tgtctattgc atggggaatc
     1741 atcgaatacg ttcataatca tccgcaactg cgagccaaga ctttgtttgc aacccactat
     1801 catgaactaa cacaactggc agagttatta ccgggtgtgc gtaactacaa cgttgcggtt
     1861 agtgaag
//
LOCUS       DAOQFT010000520         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_43963_length_1861_cov_10.278516, whole genome shotgun
            sequence.
ACCESSION   DAOQFT010000520 DAOQFT010000000
VERSION     DAOQFT010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43963_length_1861_cov_10.278516"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..571)
                     /locus_tag="PLR93_11555"
     CDS             complement(125..571)
                     /locus_tag="PLR93_11555"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNH27651.1"
                     /translation="MDTKAKLNEAMKESMKSGDELRKRTVRMVLAAIKQVEVDKRADL
                     DEMAVTALIQKEVKNRREALEEAKKANRSDLIADNEAEIKVLEEFLPKAMSADELRAL
                     VQAAITETGAAAPSDMGKVMKIVMPKVAGRAPNDAISSMVKELLNQ"
     gene            complement(617..1249)
                     /locus_tag="PLR93_11560"
     CDS             complement(617..1249)
                     /locus_tag="PLR93_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27652.1"
                     /translation="MSRMISKYHIEMTTHALAPHFSERALEIIVAANVRQDALRNQFG
                     HDEIHYDNNAIEAGDRYINEQRGLVISTLLSPGILGAWVAFGRMLHTAQDFYAHTNYI
                     TLWLDQFEGTPPPPPEIEPLKKELTQSPSFHSGKIYFPLDALYFIKPIRPWVLNFLPK
                     DSHGWMNLDTPDQGPKFDYAIAAATKRTAHEFELLQKLLPPEMFTRFTDR"
     gene            1280..1825
                     /locus_tag="PLR93_11565"
     CDS             1280..1825
                     /locus_tag="PLR93_11565"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNH27653.1"
                     /translation="MTFYTAKGDDGTTGLLGEGRVPKYHVRMEAVGTLDEASAALGLA
                     RAQCEAAQTPRILLTVQRDLYKLMAEVAATPENAEKFRGIDEEQVKWIEYETDELSKV
                     VEMPKEFILPGDTLGGAALSLARAVIRRAERRVVELFDGEVVVNPHLQRYLNRLSSLC
                     FVLELLENQHAGRKTSLAKSE"
BASE COUNT          453 a          426 c          498 g          484 t
ORIGIN      
        1 aatgaagcca gtgcaacgac ggcgatcatc aggtattgca atattcgagt acggcgtgga
       61 acgggggggc gtgtggtcat acgaacgact gcagggagac aagcgcctcc ctgcagcgag
      121 gttcttactg gttgagcagt tcttttacca tcgagctgat ggcatcattt ggggcgcgcc
      181 cggcaacttt gggcatgacg atcttcatga ccttgcccat atcactgggg gcggcagcac
      241 ccgtttcggt aatggcggct tgcactaaag ctcgcaattc atcggcagac atggctttag
      301 gaaggaattc ttctaacact ttgatttccg cttcattatc ggcaatcagg tcagagcgat
      361 tggctttctt cgcctcttcc aacgcctcac gtcggttctt cacctctttc tggatcaagg
      421 ctgtgaccgc catctcatcc aaatcagcgc gtttgtctac ttccacttgt ttaatggcag
      481 ccaataccat acggacagtg cgtttacgga gttcatctcc gctcttcatt gattctttca
      541 ttgcttcgtt aagttttgct tttgtatcca tgggtttgat tatacctttc ttgagtgacg
      601 caaaaatatt aagatgttat cggtctgtga accttgtaaa catttcagga ggtaagagtt
      661 tctgcagcaa ttcaaattcg tgggcggtgc gtttggttgc agcggcaatg gcgtagtcaa
      721 atttcggtcc ctgatcgggg gtatcgagat tcatccaacc gtgcgagtct ttgggaagga
      781 aattcaacac ccacggtcga ataggtttga taaagtacaa ggcatccaaa gggaagtata
      841 ttttcccaga gtgaaagctt gggctttggg tcagctcttt ctttagcggt tcaatttcgg
      901 gcggaggtgg aggcgtgcct tcgaattggt caagccacaa ggtgatgtaa ttggtgtggg
      961 cgtagaaatc ctgtgcggtg tgaagcatcc gcccgaaggc aacccatgcg ccgagaatgc
     1021 ccggcgaaag caaggtagaa ataaccaagc cacgctgttc attgatatag cgatctcccg
     1081 cttcaatggc attgttgtca taatgaattt catcatgccc aaattggttg cggagcgcat
     1141 cctgccgtac gttggcggca acgataatct cgagggcacg ttcgctgaaa tgtggagcaa
     1201 gcgcatgggt ggtcatttcg atatgatatt tggaaatcat gcgcgacatt ataatcactt
     1261 atccgctata atgccccgca tgacatttta tacagcaaaa ggtgacgacg gcacgaccgg
     1321 gcttctgggt gaaggacgag tgccgaaata tcatgtacgg atggaagcgg ttggcacgct
     1381 cgatgaagcc tctgccgcct tagggttggc acgcgcgcaa tgtgaagcgg ctcaaactcc
     1441 ccgaattctg ctcactgtcc aacgagattt atacaagctg atggcagagg ttgccgccac
     1501 cccagagaat gccgagaaat ttcgcggaat cgacgaagaa caagtgaagt ggattgagta
     1561 tgaaacggac gaactgagca aagtggtgga aatgcccaaa gagttcatcc tgccaggtga
     1621 tacgctgggg ggtgctgcgc tgtcactggc acgagcggtt attcgccggg cagagcgacg
     1681 tgtggtggaa ctcttcgatg gcgaagtagt tgtcaacccg catttgcaga gatatctcaa
     1741 ccgtttgtct tcattgtgct ttgtactgga actacttgag aatcaacatg ctggcagaaa
     1801 gacttctttg gcaaagtctg aataattcca aagaactttc ataaaaacag aatttcactc
     1861 t
//
LOCUS       DAOQFT010000521         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_44985_length_1837_cov_8.051627, whole genome shotgun sequence.
ACCESSION   DAOQFT010000521 DAOQFT010000000
VERSION     DAOQFT010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44985_length_1837_cov_8.051627"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLR93_11570"
     CDS             complement(<1..206)
                     /locus_tag="PLR93_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNH27654.1"
                     /translation="MATYTQNMLDYSEQIRNVTQYIREHINESLSREVLAEVAGFSVP
                     HFHRVFIATTGESAASYVRRMRLER"
     gene            388..>1837
                     /locus_tag="PLR93_11575"
     CDS             388..>1837
                     /locus_tag="PLR93_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH27655.1"
                     /translation="MSTNPITQETSLKNRPALRWTLLGVWGLVVLYTVMVLGAVLSPQ
                     FMMFRDANMARILLGIPALLQTIIDYGVDLLLLFGFSVIAVILIVNRSDDWFAILTSL
                     FLIVFGARITNLANNIALTPGYETQGGLILALGDIGIVLFTMLFPDGKFAPKWMKFLV
                     PLLIITMFGIYLFPNTSFYWVKMRDNGYYFLVTVTWYMVGLGSGIYRYYRSSTLAQKQ
                     QIRWIFIGTMGPFVWFILFQALTYLFPALTNSEVASASFQAISRLAGIFMFLMLPLFV
                     AISIVQARLFDIDMIINRSLVYGILTIGLGLTFGLVFIIASAIFRSMHASDQFFFVAV
                     IFSVAAGAGFQPLRKKLQRFVDRFFYHIQIDYQKTPLDVRAEADTNVEGGITLSSYRN
                     LKLIGHGGMAEVYRATDSTGRGTVAVKILRAALAGDEQFQKRFMREAEIVNRLEHPNI
                     VRVTDFGEERGMYYIVMEMLSGPDLNHLIRQEK"
BASE COUNT          422 a          423 c          442 g          550 t
ORIGIN      
        1 cgttcgagcc gcatgcggcg gacgtaactt gctgcactct ctcccgtggt ggcaataaat
       61 acgcggtgaa aatgcggcac agagaaccct gccacctctg ccaatacctc acggctcagg
      121 gattcattaa tatgctcgcg gatatattgg gtgacgtttc gaatctgctc agaataatcc
      181 aacatgtttt gagtgtaggt cgccatgcgg ctcaatatat ttcagagaaa gagggtctcg
      241 ctactccaaa tgtatcatga accttagaaa aaataggcaa cttttcgcct gctacagcgt
      301 aagttgacta aaccctgacc accttccaaa aataaaaaga gtgtgaagcg gttttggagt
      361 tatgattttg aaaatcaacg gcaacttatg tccacgaacc ctatcacaca agaaacctct
      421 ctgaaaaatc gtcccgcact gcgatggact ctgcttgggg tttggggatt ggtggtgttg
      481 tacaccgtta tggtgctggg agcggttctt tcgcctcagt ttatgatgtt tagagatgcg
      541 aacatggctc gcattttgct tggcatccct gcgcttttgc aaaccattat cgactatggc
      601 gtagacttgc ttcttttatt tgggttctcg gtgattgctg tgattctgat cgtcaaccgc
      661 tcagatgact ggttcgcgat cctgacctcc cttttcttga tcgtcttcgg cgctcgtatc
      721 accaaccttg caaacaatat tgcgttaact cccggttacg agacgcaggg cgggctgatc
      781 ttggcgttgg gggatatcgg cattgtgctg tttacgatgc ttttcccaga tggaaagttc
      841 gctcccaaat ggatgaagtt tcttgttccg cttctgatca ttaccatgtt tggaatttat
      901 ctttttccaa atacatcttt ctactgggta aagatgcgtg acaatggcta ttactttctg
      961 gtaactgtca cttggtatat ggttgggtta ggaagtggaa tctatcggta ttatcgctct
     1021 tctaccttgg cgcagaaaca gcagattcgt tggatcttta tcggcacgat gggaccattt
     1081 gtatggttca tcctgtttca agcgctaaca tatctcttcc ctgcactgac aaattcagaa
     1141 gttgcttctg ccagcttcca agccatttcg cgtttggctg gcatttttat gttcttaatg
     1201 ttgccgcttt ttgtggctat ttccattgta caagcgcggc tctttgatat tgatatgatc
     1261 attaaccgct cgttggtgta tgggattctc accatcgggc tgggactcac ctttgggttg
     1321 gtcttcatta tcgcctcagc aatcttccgc agcatgcatg ccagtgacca attctttttc
     1381 gtggctgtga tcttttctgt ggctgcgggc gcgggctttc agcctttacg taaaaaactt
     1441 cagcgctttg tcgaccgctt cttttatcat attcaaattg actatcagaa gactccactt
     1501 gatgtccgcg ctgaggcaga tacaaatgta gaaggcggaa ttactttatc ttcatatcgt
     1561 aatctaaagc tgattggtca cggcgggatg gcagaggtct accgcgccac agactcaacc
     1621 gggcgtggaa ccgtagcggt caaaatcctg cgagccgcat tggcgggtga tgaacaattt
     1681 cagaaacggt tcatgcgtga ggcggagatc gtcaatagac ttgaacatcc aaacatcgtg
     1741 cgagtgaccg attttggcga agaacgtgga atgtattaca ttgtgatgga aatgctttcg
     1801 ggtccagacc tcaaccatct gatccggcaa gagaagc
//
LOCUS       DAOQFT010000522         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_45288_length_1831_cov_2.909347, whole genome shotgun sequence.
ACCESSION   DAOQFT010000522 DAOQFT010000000
VERSION     DAOQFT010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45288_length_1831_cov_2.909347"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLR93_11580"
     CDS             complement(<1..394)
                     /locus_tag="PLR93_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH27656.1"
                     /translation="MAENFDVVVIGAGPAGYVAAIRAAQLKQKVAIVDKQWMGGVCLN
                     VGCIPSKSLLKNAEVAHTLRERGKDFGFTFENLKLDYSVAVKRSRQNSDRLTKGVGFL
                     MKKNNIAVFMGEGKFKSKDTLNVTDKDGK"
     gene            complement(559..1224)
                     /locus_tag="PLR93_11585"
     CDS             complement(559..1224)
                     /locus_tag="PLR93_11585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNH27657.1"
                     /translation="MPIQTSDLQLNVNDKKVYAYLADGKGPGILLLHAWWGLKPFFKQ
                     TCDRIAEQGFTVLAPDLRDRQIATTIDEAKALMEKSDGQFIGDTVFAAKAHLETLVKG
                     KLGLIGFSMGAAWSLIAAAYKPEDFGAVVLFYGNEGVDYGKITAKVMGHFCEVDEWEP
                     SEFVDNTFAEFAKAGVDATRHTYPGTAHWFVEEDRPEYDSAAALLAWRRTFEFLNSSL
                     RAR"
     gene            complement(1307..1783)
                     /locus_tag="PLR93_11590"
     CDS             complement(1307..1783)
                     /locus_tag="PLR93_11590"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNH27658.1"
                     /translation="MSKKQYDKPAINKYVHPPVVAMFFIILALLLGFFVPALAGMSQL
                     IKNIGLGLTFIGFLFGVGAFLEFRKARTTLDPHGSVKTVVTGGIYRFSRNPIYVGFLF
                     MVIGFPLAYGSLWGLVVSPMFMFALTRLVIEKEEAYLEKKFKEGYTSYKSRVRRWL"
BASE COUNT          456 a          477 c          417 g          481 t
ORIGIN      
        1 ccttgccatc tttgtctgta acattgagcg tatctttgga tttgaacttg ccttcgccca
       61 tgaacacggc gatgttgttc ttcttcatca aaaagccgac gcccttggta aggcggtctg
      121 aattctggcg cgagcgtttg accgccacgc tgtaatccag tttgagattt tcgaaggtga
      181 atccaaagtc tttgccgcgt tcgcgcaggg tgtgagccac ttctgcattc ttaagcaaag
      241 atttcgaggg aatacaccca acattcaaac aaacaccgcc catccactgc ttgtctacga
      301 tcgctacttt ctgctttaat tgtgcggcac ggatcgcggc cacgtagcct gcaggtcctg
      361 caccgatcac aactacatca aaattttcag ccatgagaaa tactctccta aatgtaaaaa
      421 aggttaaggg cgattatacc cttaaccttt ttatgtactg ttccgtcatt gcctgcaccg
      481 tgcagcaggc acgcgtgcga gggcgctctt gttcttcgcc cgaagcaatc cccatattat
      541 gtttgaggtt gcttcgccct atcgggctcg caatgacgaa ttgagaaatt caaatgtgcg
      601 tctccatgcc agcaacgcgg cagcggagtc gtattcgggg cgatcttctt ccacgaacca
      661 atgggcagtt cccgggtagg tatgacgcgt cgcatccaca ccagcttttg cgaactcggc
      721 aaaggtgtta tccacaaatt cgcttggctc ccattcatcc acttcgcaga aatgccccat
      781 cactttggca gtgatcttgc catagtccac gccttcgttg ccgtagaaga gaacgaccgc
      841 gccgaaatct tctggcttat acgcggcagc gataagcgac catgctgccc ccattgaaaa
      901 accgatcaac ccaagtttgc ctttcaccag cgtttccaaa tgtgccttcg cagcgaagac
      961 cgtgtcgccg ataaattgac cgtcactttt ttccatcaag gctttggctt catcgatggt
     1021 cgtggcgatt tgtctatcac gcagatcagg tgcgagaacg gtaaagcctt gttctgcaat
     1081 tcggtcacag gtctgtttga agaatggctt caaaccccac caggcgtgca ataataatat
     1141 gccgggaccc tttccgtctg cgaggtaggc atacactttt ttatcgttca cgttcaattg
     1201 aaggtcagat gtttgaatag gcatggtaat ctcctcaaaa tgattataag tacatttgtt
     1261 ctaaaataca agcaaaagaa aaaggcgact ttcatcgcct ttggatttac aaccagcgcc
     1321 tcactctcga cttgtagctg gtgtagccct ctttgaattt tttctccaag taggcttctt
     1381 ctttctctat gacaagccgt gtgagcgcga acatgaacat gggcgatacg accaagcccc
     1441 acagtgaccc atatgcaagc ggaaagccga tcaccatgaa gagaaaccct acatagatcg
     1501 ggttgcgcga gaaacgataa ataccgcctg tcactacagt tttgaccgag ccatgcgggt
     1561 cgagggttgt gcgagctttg cgaaattcta aaaatgcacc cacaccaaaa aggaaaccga
     1621 tgaaggttaa tcccaacccg atatttttta tcagttgcga catgccagcc aatgccggga
     1681 cgaaaaatcc caagagcagg gcaaggatga taaagaacat tgccaccaca ggcgggtgga
     1741 catatttgtt gatcgctggt ttgtcgtatt gttttttgct catctcattc ctcgatttca
     1801 agtgttgctt tcaacggcgg tttgcgccag a
//
LOCUS       DAOQFT010000523         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_45427_length_1827_cov_6.252822, whole genome shotgun sequence.
ACCESSION   DAOQFT010000523 DAOQFT010000000
VERSION     DAOQFT010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45427_length_1827_cov_6.252822"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..825
                     /gene="fabG"
                     /locus_tag="PLR93_11595"
     CDS             67..825
                     /gene="fabG"
                     /locus_tag="PLR93_11595"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNH27659.1"
                     /translation="MSFKLSLENKVAIVTGGSRGIGRAIALEFADRGAAVVVNYNKSP
                     ESAEEVVKKIQEAGGKAAAFQADVSDFKQAEALVKFAIDTFGDLHILVNNAGITRDQL
                     IMMMPEADWDAVINTNLKSTFNCSKAAVKHMMRKRTGRIINMASVAGQMGNPGQTNYS
                     ASKGGQIAFTKALAREVSARNITVNAIAPGFVDTEILDAMTPEALEAALKLVPLGRKG
                     KPEEIAFTATFLASDGAAYITGQVIGVDGGMAMM"
     gene            839..1186
                     /locus_tag="PLR93_11600"
     CDS             839..1186
                     /locus_tag="PLR93_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNH27660.1"
                     /translation="MTESTQGKTTVSPEVLTTIASLAALEVPGVSRLAPVSGGVNRLF
                     RRGTSDGVRIEAKDNTVFVDLFLILKDGVNIREVGRNVQANVARSVQEMVGMEVGEVN
                     VHIEDIDFEGDEA"
     gene            1191..1649
                     /gene="nusB"
                     /locus_tag="PLR93_11605"
     CDS             1191..1649
                     /gene="nusB"
                     /locus_tag="PLR93_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNH27661.1"
                     /translation="MKPRTRARALALQVLYETDLSTHLPGDVLKTRLEETTLPQDLAE
                     FARSIIFGILPIRENLDRVIAKYAPEWPLDQIAAIDRNILRMACWEFAISRQTPVKVA
                     INEAVELAKLFGSDSTARFVNGVLGTLVEHEQEIYQVLKNEIPQETNLES"
BASE COUNT          483 a          441 c          449 g          454 t
ORIGIN      
        1 catctatttg atttcaattt ttactcaatt ccagatgaag tataatttct ttacaggagc
       61 ccagttatgt ccttcaaatt atcattagag aataaagtag ctatcgttac cggtggttca
      121 cgcggaattg gacgcgccat cgctctcgaa ttcgctgatc gcggcgcagc ggtggtggtg
      181 aattacaaca aatcccctga gtcggctgaa gaagtggtga agaaaattca agaagcgggt
      241 ggaaaagccg ccgcctttca agctgatgtc tccgatttta agcaagccga ggcgttggtt
      301 aagtttgcca ttgatacttt tggcgacttg catattcttg tcaataatgc cggcattacg
      361 cgcgaccagc tcattatgat gatgcccgaa gcagactggg atgccgttat caacaccaat
      421 ctcaaaagca ccttcaattg ctcaaaagct gccgttaagc acatgatgcg caaacgcacc
      481 gggcgcatca tcaacatggc atcggttgct gggcaaatgg gcaaccccgg gcaaacaaac
      541 tattccgcct ccaaaggtgg tcagatcgca tttaccaaag cattggcacg cgaagtctct
      601 gctcgtaaca ttaccgtcaa cgctatcgcg ccgggatttg tagatactga gattctcgat
      661 gccatgacac ccgaagccct tgaagctgcg ctcaaacttg tgccgcttgg gcgcaaagga
      721 aaacccgaag aaattgcctt tactgctacc tttctcgcct ctgacggcgc cgcgtacatt
      781 accggtcagg tcattggtgt ggacggcggc atggcaatga tgtaaaacag gagtaataat
      841 gactgaatct acgcaaggaa aaactaccgt ctctcccgaa gtgttgacaa ctatcgccag
      901 tctcgccgca ttggaagtcc ccggcgttag ccgactggca ccagtcagtg gcggcgtgaa
      961 tcggctcttt cggcgcggca ccagtgacgg cgtccgcatt gaagcgaaag acaacacggt
     1021 ctttgttgac ctattcctca tcctcaaaga tggagtcaac atccgcgaag ttgggcgcaa
     1081 tgtgcaagcg aatgtagccc gttccgttca ggaaatggtt ggcatggaag tgggcgaagt
     1141 gaatgtccac atcgaagata ttgattttga aggggacgag gcataatcaa atgaagcccc
     1201 gaacccgggc gcgcgcgctc gccctgcaag tattgtacga aactgatctt tccacacacc
     1261 tgcccggtga tgtattgaaa actcgtttag aagaaaccac tctgccgcaa gatttagcag
     1321 agttcgcccg cagcattatt tttggtattt tgcccattcg agaaaacctt gaccgcgtga
     1381 tcgcaaagta tgcacctgag tggccactcg atcaaattgc cgccatcgat agaaatattt
     1441 tgcgtatggc atgttgggaa tttgcaatct cgcgccaaac tccggtaaag gttgcgatca
     1501 atgaagctgt agagttagcc aagttgttcg ggtctgactc gactgcccgc tttgtaaatg
     1561 gtgtgcttgg taccttggtt gagcatgagc aggaaatcta tcaggttctg aaaaacgaaa
     1621 tacctcaaga aaccaatttg gaatcataaa catggaaatc cttggcattg gaatgcctga
     1681 acttattttt gtcgtgatta ttgctttgct tattttgggt ccgaaagata tgcaaaaagc
     1741 cggcaagacc atcggaaagt ttttacgaga tatcatcaca tctgatggct ggaaactttt
     1801 tcaacaaacc tcgcgcgact tgcgcac
//
LOCUS       DAOQFT010000524         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_46191_length_1810_cov_4.821083, whole genome shotgun sequence.
ACCESSION   DAOQFT010000524 DAOQFT010000000
VERSION     DAOQFT010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46191_length_1810_cov_4.821083"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLR93_11610"
     CDS             <1..224
                     /locus_tag="PLR93_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27662.1"
                     /translation="NSITPDMTQYHAFSEIDPNTITAIIETGFLYLDREILTKQTDVV
                     AEGVTQGILCFVNNESVLPNPVNLPTILP"
     gene            221..898
                     /locus_tag="PLR93_11615"
     CDS             221..898
                     /locus_tag="PLR93_11615"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNH27663.1"
                     /translation="MTSRIAGMIFGLLIFLVGAIAWTVTTSEALAPIPNVTPTYGKPF
                     AVAEILKPTYTPLPPPTLAPTQEPSLTPQALVEAVIVDSPEQVNAAEVEQAPVNYSGG
                     KKILVDISEQHMYVYEGEQIVYSFVASTGMNNATRTGTFSVLDKIPNAYGATWNIWMP
                     NWLGIYWAGSLENGIHALPILPGGATLWAGYLGTPISYGCVVLDTYDAQVLYDWAEVG
                     VPVEIQW"
     gene            984..>1810
                     /locus_tag="PLR93_11620"
     CDS             984..>1810
                     /locus_tag="PLR93_11620"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNH27664.1"
                     /translation="MNNRLTNSQAPAQTGGKSPKNGNPAKGPAKKRNLILPIVLVITG
                     CVVFGFAAWSAVTSPVMASILNRGSNSAEFQQVTPGQSFAQVYIAKPTYTPMGLEPVA
                     QQVVVQPTVTFTPTSQAELAVVQPTDPPTIEAAAEVVVQPTATTESVAVVSTGEAVAQ
                     AQIIADTPTSEYVAPPTAVANAPAPVSSPGNGVRWFDVDLSDQRMYAYEGDTLVRTFV
                     VSTGTWQTPTVTGRFKVWIKLKSAPMSGPGYYLPDVPYIMYFYGDYGIHGTYWHNNFG
                     "
BASE COUNT          447 a          486 c          433 g          444 t
ORIGIN      
        1 ccaacagcat cacgcccgat atgacccaat atcacgcctt ctcagaaatt gacccaaaca
       61 ctatcacagc catcatcgaa accgggttct tatatctcga ccgtgagatt ctcaccaaac
      121 aaactgatgt ggtagccgaa ggcgtaacac aaggcatctt gtgtttcgtc aacaacgaaa
      181 gtgttttgcc caatcctgtt aacctgccca ccattctgcc atgacctctc gcattgccgg
      241 tatgattttt gggttgctta tttttctagt aggcgccatc gcatggaccg ttacaacctc
      301 tgaagctctt gctccgattc caaacgtcac accgacctat ggcaaaccct ttgccgtagc
      361 tgaaatactc aaaccgacgt acacgcctct ccctccaccg acgcttgctc ctacccagga
      421 gccttcactt acgccgcaag cgttagtcga agccgtgatc gtggattctc ccgaacaagt
      481 caacgcagcg gaagtagagc aggcgccggt caactatagt ggcggaaaaa aaatcctggt
      541 agatatttct gaacagcata tgtacgtcta tgaaggggaa caaatcgtct acagcttcgt
      601 cgcttctaca ggcatgaaca acgccacccg caccggaact tttagtgtgc tcgataaaat
      661 ccccaacgcc tacggtgcta catggaatat ctggatgcct aactggcttg gtatttattg
      721 ggcgggcagt ttggagaacg gcatccacgc tttgcccatc ctgccgggcg gagccaccct
      781 ctgggctggc tatttgggaa ctccaatttc gtatggttgt gtcgtcttgg atacatacga
      841 tgcacaagtg ctgtatgact gggcagaggt cggcgtgcct gttgaaattc aatggtgaaa
      901 ttggaatctt tttgtttata atcttatata gctcgtttgt ttcgtaattg tccttattcg
      961 ttgatgcctc aaaaggtgac cccatgaaca accgtctcac aaattcacaa gcgcccgctc
     1021 aaacgggtgg caaaagccca aagaacggca acccggcgaa gggtccggca aagaagcgga
     1081 atctcatcct gccgattgtc ttggtcatta cagggtgtgt tgtttttggc ttcgcggcat
     1141 ggtcggcagt cacatcgcct gtgatggcat cgattttgaa tcgcggctca aatagcgcag
     1201 aattccagca agtgacaccc gggcagtctt ttgcgcaggt gtatattgcc aagccaacat
     1261 atacccctat ggggctggaa ccggtagcgc aacaagtcgt tgtgcaacca acagtgacat
     1321 ttacacccac ctctcaagca gagttggcag ttgtgcagcc tacagaccca ccaacaatag
     1381 aagcggcggc agaagtggtt gtgcaaccca cggctactac tgaatcggtt gcggttgtat
     1441 ctacaggtga agcagtagct caagctcaga tcatcgcaga tacaccaact tcagaatatg
     1501 ttgcgccacc tacggctgtt gcaaacgcac cagcaccggt cagctcgccg ggcaatgggg
     1561 tacgttggtt cgatgtggac ctttcggatc agcgcatgta cgcttacgaa ggcgatacct
     1621 tggtacggac ctttgtggtt tctacaggca cctggcaaac ccccaccgta accggacgtt
     1681 tcaaagtgtg gatcaagcta aaatctgcgc caatgtcggg tccgggttac tacttgccgg
     1741 atgttcctta cattatgtat ttctatggcg actatggaat tcacggcacc tactggcata
     1801 acaactttgg
//
LOCUS       DAOQFT010000525         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_46355_length_1806_cov_4.904626, whole genome shotgun sequence.
ACCESSION   DAOQFT010000525 DAOQFT010000000
VERSION     DAOQFT010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46355_length_1806_cov_4.904626"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1070
                     /locus_tag="PLR93_11625"
     CDS             48..1070
                     /locus_tag="PLR93_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNH27665.1"
                     /translation="MNIQRVDSLNYPALESQLSYIGLDSWMNFIHDVYGHPVHRFVAL
                     DSNRPLGALSLLEVKSPVFGHYLATAPFGNYGGFAFENHEARNLLLDEAQRLAQDIKA
                     EYVSIRFDDIASPPVDSWVEHPVYSTYLINLSATPDDLLKRFSSDHRNHVRKSLKKGH
                     AIRFGHLDLLNDAYEGLARSMHELGSPYHSKTYLRKMAEYLGNTLEFAVAYDPKGKIT
                     GSGVFIYQGKTIFNLHANVLRFARSSYAGEFLYWSVIEHAMQKGCTTFDLGRSLIGSG
                     NDAFKSKWAPEKKPHAYWYWLAPGHELPSLNQKNPKFQFAIATWKRLPSFIVRPLGPY
                     IIGGLV"
     gene            1075..1674
                     /locus_tag="PLR93_11630"
     CDS             1075..1674
                     /locus_tag="PLR93_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH27666.1"
                     /translation="MSHTIYPLKQISSDTLPRIAEIHMGDAGLLSKLGHPFVLKYFEY
                     AIKDTRAFGFYAQEDETGEIMGFSLASPEPSSLTSKLTEDKGWFIKNIFKVVFTRPLA
                     FLQMVISSITIRGQMYEPNSIECVYFTVDPKFRGHGLGRSLQKALMDEGRKQGYKKIY
                     ASVETWNIASLKATQANGLKIIKTFREGIYYRHRLESEL"
BASE COUNT          474 a          464 c          383 g          485 t
ORIGIN      
        1 aagcatttcc caacctccac tatgcaagat tacatcaaag agcttccatg aacatccaac
       61 gagttgactc tcttaactat ccagcccttg aatcccaact gagttacatt ggtctcgact
      121 catggatgaa tttcattcat gatgtatacg ggcatcctgt ccatcgtttc gttgctctcg
      181 atagtaatcg cccgcttggt gcgctttcat tgcttgaggt gaagagtccc gtttttggtc
      241 attatcttgc tacagcacct tttggtaact atggcggctt cgcatttgaa aaccatgaag
      301 cgcgtaatct tttattggat gaagcccagc gacttgcaca ggatatcaaa gccgagtacg
      361 tttctattcg atttgacgat atcgcctctc cccccgtcga cagctgggtt gaacaccccg
      421 tttactctac ctatctgata aatctctctg ccactccaga cgacctgctc aagcgtttct
      481 catccgacca tcgcaatcac gttcgcaaat ctctcaaaaa gggacatgcc attcgctttg
      541 gtcatctcga cctattgaat gatgcttacg aaggtctcgc aagaagtatg cacgaattag
      601 gttcacctta tcactccaag acttatcttc gcaagatggc tgaatatctt ggaaatacgc
      661 tcgaatttgc cgtggcctat gaccctaaag gcaaaatcac tggcagtggt gttttcattt
      721 accaaggtaa gaccatcttt aacttacacg caaacgtact tcgatttgca cgttcatcct
      781 atgcaggtga attcttgtac tggtctgtga tcgaacatgc catgcaaaag ggatgcacca
      841 cttttgacct cggtcgcagt ttgattggtt cagggaatga cgcatttaaa tccaaatggg
      901 caccagagaa aaaaccgcac gcatattggt attggcttgc accggggcat gaattgccat
      961 cactcaatca gaagaatcct aaattccaat ttgccattgc cacttggaag cgccttccca
     1021 gttttatcgt ccgcccgttg ggaccttata tcatcggtgg attggtgtag tcctatgtcg
     1081 catacgattt accctcttaa acaaatttcg tctgatacac ttccacgcat cgctgaaata
     1141 catatgggtg atgctggttt gctctccaag ttggggcatc cctttgtgct caagtacttt
     1201 gagtacgcaa tcaaggacac acgcgccttt ggtttttacg ctcaagaaga tgagacgggt
     1261 gaaattatgg gttttagcct tgcctcaccc gaaccttcat cactgacatc taaactcact
     1321 gaagataaag gctggttcat caagaacatc ttcaaagttg tttttacccg tccgctagct
     1381 tttttgcaga tggtcatttc cagcatcacc atccgcggac agatgtatga gccgaacagt
     1441 atcgaatgtg tttatttcac cgtcgacccg aaattccgcg ggcacggact gggacgctcc
     1501 ctgcaaaaag ccctcatgga cgagggacgt aaacaggggt ataaaaaaat ctacgccagc
     1561 gtagaaactt ggaacatcgc cagcttgaaa gccacgcaag cgaatggatt gaaaatcatc
     1621 aagaccttta gagaaggtat ttattacagg caccgcctgg aaagcgagtt gtaagagcat
     1681 gaccacccat cgagaacgca ttcaagcctg ttttgaaggt gagcagaccg accgaactcc
     1741 catcgccctt tggcggcact tccctgaaga agaccaaaat ccggaaacgc ttgccgccgc
     1801 cacgat
//
LOCUS       DAOQFT010000526         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_46396_length_1805_cov_6.442857, whole genome shotgun sequence.
ACCESSION   DAOQFT010000526 DAOQFT010000000
VERSION     DAOQFT010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46396_length_1805_cov_6.442857"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLR93_11635"
     CDS             <1..894
                     /locus_tag="PLR93_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNH27667.1"
                     /translation="VEDTDFNAKSAGTTAPKTVTFSKQMKSGENLRLRGADIRTGDAV
                     LKRGRTLKPQDLGLLATLGHAHVTVHKKARVALLSSGDELLDVSAPLEPGKIRDSNSY
                     ALAAAVSNLGVDVIRLGVARDTRESITQLLDQAASENADLILSSAGVSVGAFDYIKEV
                     IDANGKMDFWRVNMRPGKPLAFGEYRGIPFIGLPGNPVSAFVGFEVFVRPVLERLNGK
                     MDGGRQTVRVRCEEEIESDGRESYLRAKIQVKNDIRIAILTGHQGSGNLLSLVQADAL
                     LIIPAGVKCVPAGSEVDAILL"
     gene            901..1326
                     /locus_tag="PLR93_11640"
     CDS             901..1326
                     /locus_tag="PLR93_11640"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNH27668.1"
                     /translation="MDKWLYRVMIALVIIGLAVSVYMTIYKISGNDGMCLGSGDCSTV
                     NASRFSEVNGIPVAVFGIVGYAAILIVLFYENKNEFFKRNGTLLAFGMSLTGFLFTLW
                     LVYVELVLLKAICPFCVTSQVSMTLIFIIAVVRLFRNEN"
     gene            1338..1568
                     /locus_tag="PLR93_11645"
     CDS             1338..1568
                     /locus_tag="PLR93_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27669.1"
                     /translation="MPRIKCHYVDCVFLDEGLCGAAAVEFDPDTGCVTYSPADGAAAE
                     DEWEDEEELDEWEDEDADGEEDDEWLEEEDEF"
BASE COUNT          479 a          399 c          456 g          471 t
ORIGIN      
        1 gtagaagaca cagatttcaa cgcgaaaagc gcaggaacca ccgcccccaa gacagtcacc
       61 ttctccaagc agatgaaatc aggagagaac cttcgcctgc gaggcgcaga tatccgcact
      121 ggagatgctg tgctgaaaag gggtcgcact ctcaaaccgc aggacttggg tctgctcgcc
      181 acacttggac atgcacatgt aacggtccac aagaaagcgc gagtggcatt gttatcctct
      241 ggagatgagt tattggatgt aagcgctccg ttggaacccg gcaagatccg tgactctaat
      301 tcctatgcct tggcagccgc agtcagcaac ctaggcgtag atgtcattcg gctgggtgtt
      361 gcaagagata ctcgcgaatc tatcacccaa cttttggatc aagccgcaag tgaaaacgca
      421 gaccttatcc tttcctctgc cggggtgagt gtaggcgctt ttgactacat caaagaagtg
      481 atcgatgcca acggcaaaat ggatttttgg cgggttaaca tgcgcccagg caaaccattg
      541 gcatttggcg aatatcgggg aattccgttc attgggctgc cgggcaaccc cgtttcagct
      601 tttgttggct tcgaggtttt tgtgcgcccc gtgcttgaac ggctgaacgg caaaatggac
      661 ggaggcaggc aaacggtccg cgtacgatgc gaagaagaaa tcgaatcaga tggtcgcgag
      721 agttatctgc gggcaaaaat ccaagtaaaa aatgacattc gtattgcaat tttgaccgga
      781 caccaaggct caggtaacct tttgtcacta gtacaggcgg atgctttact aatcatcccc
      841 gctggtgtaa aatgcgtgcc tgctggttcg gaagtagacg ctattctatt gtgaggaacc
      901 atggataaat ggctttatcg cgtgatgatc gcgcttgtaa tcatcggttt ggcagtgtca
      961 gtttatatga caatttataa gatcagtgga aacgatggca tgtgcctcgg ctctggtgat
     1021 tgctccactg tgaatgccag tcgcttttca gaagtgaacg gaattcctgt agcagtattt
     1081 ggtattgtcg gctacgctgc catcttgatc gttttgttct acgagaacaa aaatgaattt
     1141 ttcaaaagga acggaacctt gcttgccttt ggcatgtcac ttaccggttt cttgttcacg
     1201 ctttggttgg tatatgttga acttgtattg ctcaaagcga tctgcccgtt ctgcgtcacc
     1261 tcgcaagtct ctatgacgtt gatctttatt attgcggttg tacgactatt ccgaaacgag
     1321 aattaggagg attcgaaatg ccccgtatta aatgtcacta tgttgattgt gtctttttgg
     1381 atgaaggatt gtgcggcgca gcagccgtag aattcgaccc agacacaggt tgtgtgacct
     1441 attcccctgc cgatggcgcc gcagcggaag atgaatggga agatgaagaa gagctcgacg
     1501 agtgggagga tgaagacgcc gatggcgaag aagacgacga gtggctcgaa gaagaagatg
     1561 aattctagcc cgcttcattc ttaactcaaa actgattcta caaaaaagcc ttctctatgt
     1621 gagagggctt tttcttaacc aggcgcaagt atgtataatg ctccaattgt tatccctgaa
     1681 attttatcaa gagtataaaa tagggaaaat cacaaaaagg gcatcttatg ttaacactta
     1741 caaaaaccaa ctcagaaaat aaagatttta ttcatctcat ccgcttttta gatgcagacc
     1801 ttgcc
//
LOCUS       DAOQFT010000527         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_46640_length_1799_cov_6.116399, whole genome shotgun sequence.
ACCESSION   DAOQFT010000527 DAOQFT010000000
VERSION     DAOQFT010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46640_length_1799_cov_6.116399"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLR93_11650"
     CDS             <1..647
                     /locus_tag="PLR93_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH27670.1"
                     /translation="RLRTDRRTAQIPIIFLTEKRDRADRLQGLELGADDYITKPFDVQ
                     ELRLRVRNALKRVSQGSLTNPVTGLPEGALVDEKLSEVLGTDNSSLLFISLLNMSAFR
                     EAYGFVASDDVLRAVSLMIVNTMREFSRPEDFLGHLSTTEFILVVPPANLAALSDKLN
                     SRLEQSVEYFYPIKDREQAAKRSDRLGAKLIEVASLKTYTDVVKFKAELLSFKN"
     gene            657..1397
                     /locus_tag="PLR93_11655"
     CDS             657..1397
                     /locus_tag="PLR93_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNH27671.1"
                     /translation="MRITVVIPTYNEAENLPKLVSALFSLPLDLSLLVVDDNSPDGTG
                     QLAEDLAKQHPGRIDVLRRPGKMGLRSAYLNGFQRILDSNADAIVQMDADFSHDPVVL
                     TAMAKLLETNDVVLGSRYVSGGSVDEKWPVWRKWLSAFGNFYARTILGLPLHDVTTGY
                     RMWRSTTLKQMPFERIQATGYIFLVEMIYLAHCLKFQIGEAPIYFADRRFGKSKMSFK
                     IQMEAAVRIWQVLWNYRDLRKAGKSARI"
     gene            1520..>1799
                     /locus_tag="PLR93_11660"
     CDS             1520..>1799
                     /locus_tag="PLR93_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH27672.1"
                     /translation="MIIERNDLRNIAIIAHVDHGKTTLVDGLLRQSHTFRENQHVEER
                     VMDSNDLERERGITILAKNTAISLVDPATNQPIKINIVDTPGHADFGGE"
BASE COUNT          478 a          437 c          413 g          471 t
ORIGIN      
        1 gccgccttcg caccgatcgt cgaaccgctc aaatcccaat cattttctta accgaaaaaa
       61 gagaccgagc agaccgcttg caagggctgg agcttggtgc tgatgattac atcaccaagc
      121 cttttgacgt gcaggaattg cgcctgcgtg tgcgaaatgc tttgaaacgc gtcagtcagg
      181 gttcgttgac caaccctgtt accgggttgc cagaaggcgc gctcgtagac gaaaagttat
      241 ctgaagtcct tggaacagat aattcatcgc ttctattcat atccttattg aatatgagcg
      301 catttcgtga agcctacggt ttcgtagcct cagatgatgt gttgcgagcc gtcagtttga
      361 tgatcgtcaa cactatgcgt gagtttagcc gcccagaaga tttccttggg cacctgagca
      421 ccacagaatt tattctggtt gttccccctg cgaaccttgc tgccttgagc gataaactta
      481 attcccgcct tgaacagtcg gtagaatatt tttacccgat caaagatcgt gagcaggcag
      541 ccaaacgatc agaccgcctt ggtgctaaac tgatcgaagt tgcctccctc aagacatata
      601 cggatgttgt caaattcaaa gcggagttgc tcagctttaa gaactaggaa agtacattgc
      661 gtatcacagt tgttattccc acctataacg aagcggagaa cctacccaag ttggtctccg
      721 ctttattttc gcttccacta gatttaagcc tgcttgtcgt agacgacaat agccccgatg
      781 gaacgggtca actggctgaa gaccttgcaa aacagcaccc cgggcggatt gatgttttac
      841 gccgtccagg caaaatgggg ctccgctctg cctacctgaa cggatttcaa aggatactcg
      901 acagcaatgc cgatgccatt gtccagatgg atgcggactt ttctcatgat cccgttgtgt
      961 tgacagcgat ggcaaagtta cttgagacca acgatgttgt tcttgggtct cggtatgttt
     1021 cgggcggctc tgtggatgag aaatggcccg tttggcgaaa atggctttct gcatttggta
     1081 atttctatgc gcggacgatt ttaggactcc ccttacatga tgtaaccacg ggctatcgta
     1141 tgtggcgtag cacaaccctc aagcaaatgc cttttgaacg cattcaagca acaggttaca
     1201 tttttctagt tgaaatgatc tatttagctc attgcctcaa gtttcagatt ggtgaagccc
     1261 caatttattt tgcggaccgt cgttttggaa aatccaaaat gtctttcaaa atccagatgg
     1321 aagcggcagt acgtatctgg caggttcttt ggaactaccg agatttgcgc aaagctggaa
     1381 aatcggcaag aatctagtct cccttcctca aactgacgct ctctgataaa atactgcccg
     1441 caagccgaac gaaaaattac cccctcggcg aaatgtcgag ggatttttgt gcctgtatat
     1501 aattgaagaa agaataaaaa tgatcattga acgaaacgac ctcagaaata ttgccattat
     1561 tgcacacgtt gaccacggaa aaaccactct cgtagatgga ctcctacgtc aatctcatac
     1621 cttccgcgaa aatcaacatg tcgaagaacg tgtgatggat tcgaacgacc ttgaacgcga
     1681 acgcggcatc accattctcg cgaagaacac tgctattagc ttagtagacc ctgctactaa
     1741 tcaaccgatt aagattaaca tcgtagacac tcccggacat gccgattttg gcggggaag
//
LOCUS       DAOQFT010000528         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_46677_length_1798_cov_7.924842, whole genome shotgun sequence.
ACCESSION   DAOQFT010000528 DAOQFT010000000
VERSION     DAOQFT010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46677_length_1798_cov_7.924842"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1258
                     /locus_tag="PLR93_11665"
     CDS             179..1258
                     /locus_tag="PLR93_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27673.1"
                     /translation="MKLNHYPVPFLFLAILGLLAALWAGLMRLGWQLPALTPSLAMLH
                     GPVMISGFLGTLITLERAVAMKQKWMYLPPLLSGLGWLIATILPKLPFGMVLLTLASL
                     GGVAILAEIARREFALHTVTMLLGAIAWLTGNLLWMFGWQINQVVFFWMAFLVLTIAG
                     ERLELSRVLRPTQMQQILFGSIVFIFLAGIILSLFNLQFGTRLSGLGLLLFPLWSLRN
                     DIAWRNIRHKLPLTRYIAWCLALGFLWLGISGGLSLGLGAQVAGPRYDAMLHTVFVGF
                     VISMIFGHAPIIFPAILGIPINFQPVFYIHLGLLHISLLLRVIADYANLFTLRMWGGL
                     LNEIAIFIFIALTVISVRKGMIGKA"
     gene            1394..>1798
                     /locus_tag="PLR93_11670"
     CDS             1394..>1798
                     /locus_tag="PLR93_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27674.1"
                     /translation="MKKYILLLLTLTLLIPTASVLAEGQFDYIVIRGFGIVEDINISN
                     PALTQDYYAFADFSKGEVNPPSDPGIGYQVVRMYAKDSKGIPYDQLHYYPDSGYVFYD
                     GIVNGYSELGTKWYLANPTVEEPFRAALAENAR"
BASE COUNT          400 a          459 c          399 g          540 t
ORIGIN      
        1 caacccatgt tggaaggctt ttttacaacc ttcccaagtc acgttggtcc gcacctggct
       61 gaaggaggta cgatttacgc ttttcacctt caaagcgcag tcaataatag gttattgata
      121 atataatcaa gcatccttca acaactatcg gcagacaaca cgaaagccct tcattaaaat
      181 gaaactcaac cactaccctg tcccctttct ctttctcgcc atccttggtc tgctcgctgc
      241 actatgggca ggtctcatgc gccttggctg gcaattgccc gctctcaccc cgtcccttgc
      301 catgctgcat ggtcccgtga tgatctctgg atttttggga acattgatca cattggaacg
      361 tgccgtggca atgaaacaaa agtggatgta cctgcccccg cttctttctg gattgggttg
      421 gctgattgcc accattttgc cgaagcttcc tttcggcatg gttctcctaa cccttgccag
      481 cctcggcgga gttgccattc tggcggagat cgcccgccgc gaatttgcat tacacactgt
      541 caccatgtta ctcggtgcaa ttgcatggct gacaggaaat cttttatgga tgttcggctg
      601 gcagatcaat caagttgtat ttttctggat ggctttcctt gtgctgacca ttgcaggtga
      661 acggctcgaa ctcagccgtg ttttgcgtcc tacacaaatg caacagattc tctttggcag
      721 tattgtgttc atttttctgg caggcattat cctatccctg tttaatctgc aattcggcac
      781 acgcctgagc ggactgggct tgctgttgtt cccgctttgg tcgctgcgta acgatatcgc
      841 ctggcgcaac attcggcaca aacttccgct cacgcgctac attgcctggt gcctcgcact
      901 tggctttctt tggctcggca tcagtggcgg attaagcctg gggcttggcg cacaggttgc
      961 agggccaagg tatgatgcga tgcttcacac ggtttttgtt ggcttcgtca tttctatgat
     1021 ctttggtcac gcccccatta tctttcctgc tattttggga atcccgatca actttcagcc
     1081 tgtattttat atccacttgg ggcttttgca catttcgctg ttgctgcgcg tgatcgccga
     1141 ttacgccaat ctcttcacat tgcgtatgtg gggcggattg ttaaatgaga ttgcgatttt
     1201 tatttttatt gcactaacgg ttatctcagt gcgaaaaggc atgatcggaa aagcctgata
     1261 attttcaagc aaatttcgat tcatctttgc ttaagcgcaa agatgaattt tttatttcag
     1321 ggataattca atcacaaccc tattaagcgg acaggttttc acatacaata gaccaaatta
     1381 aaactggaga cttatgaaaa aatatatctt gctcttatta actctaacct tgctgattcc
     1441 caccgcctcc gttcttgccg agggacaatt tgactacatc gtcattcgcg ggtttggaat
     1501 tgtcgaagat atcaacatat ccaaccctgc gctgacacag gattattatg cttttgctga
     1561 tttttccaaa ggcgaagtca acccgccctc cgaccctgga attggttatc aagtcgtccg
     1621 tatgtatgcc aaagacagca agggcattcc ctacgaccaa ttgcattatt accccgacag
     1681 cggatatgtt ttttacgacg gcatcgtgaa tggatattct gaattgggca cgaaatggta
     1741 tcttgccaac cccacggttg aagaaccttt ccgcgcggcg ctggctgaaa atgcgcgg
//
LOCUS       DAOQFT010000529         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_46680_length_1798_cov_6.783133, whole genome shotgun sequence.
ACCESSION   DAOQFT010000529 DAOQFT010000000
VERSION     DAOQFT010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46680_length_1798_cov_6.783133"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /gene="topA"
                     /locus_tag="PLR93_11675"
     CDS             complement(<1..1651)
                     /gene="topA"
                     /locus_tag="PLR93_11675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNH27675.1"
                     /translation="MEAYCMKCKTKREMQDPVAGFNAKSSAVTTGTCPVCGTKLFRMG
                     KTDAHAGLIPPPKPEKVEVREGKLVIVESPAKAKTVGRFLGKGYTVRASVGHVRDLLK
                     SQLSVDVDNNFEPKYRVPNEKKDVVKEIKQLAKKAEEIFLATDPDREGESISWHLMEA
                     AGIEPERTKRVVFHEITAPAVADAFSHPRNIDMNLVNAQQARRVLDRLVGYSISPILW
                     EKVRGRLSAGRVQSVALRLIVDREREIDAFKPVEYWTIHAEFKPEKLKSNFIAKLVRV
                     DDKEPELPSKETVEPLLYDLETASFSISKVKRGERRRKPAAPFTTSTLQQEASRKLGY
                     TARRTMALAQGLYEGQDVGEGGTTGLITYMRTDSTNVSAIAQSETREFVTGKYGGDFL
                     PAEPPTYKTKSANAQEAHEAIRPTSVMRDPEKVKEFLEPAMYKLYRLIWQRFVASQME
                     AAVYDTLQVEVTGKTNEHEYLLRASGSAIKFPGFMVVYEEAKNEDAKVEEDEEDVKIP
                     AGVAEGQKQELVRLIPEQHFTQPPPRFSEASLVQALEENGIG"
BASE COUNT          434 a          468 c          419 g          477 t
ORIGIN      
        1 gaccaatccc attctcttcc agggcttgta caagcgatgc ttctgaaaag cgtggaggag
       61 gttgagtaaa gtgctgttca ggaattaaac ggaccaattc ctgcttttgt ccttccgcca
      121 caccggcggg aatctttaca tcttcttcgt cttcttcaac cttggcatct tcatttttgg
      181 cttcttcgta aacaaccata aagccaggga acttaatagc agacccagaa gcacgcaaga
      241 gatattcgtg ttcgttcgtc ttgccggtca cttcaacctg caaagtgtcg tatacagcgg
      301 cttccatctg agaggcaaca aagcgttgcc agatcaagcg atatagcttg tacatggcgg
      361 gttcaaggaa ttccttgacc ttttccgggt cacgcatcac agatgtaggg cgaatggctt
      421 cgtgagcttc ctgtgcattc gcagacttgg ttttgtatgt aggcggttct gccggaagaa
      481 agtcgccgcc atatttaccg gtgacaaact ctcttgtttc gctttgagca atagcagaga
      541 cattggtcga gtcggtacgc atataggtga taagacctgt cgtaccaccc tcgcctacat
      601 cctgaccttc ataaagcccc tgcgccaacg ccatggtacg acgggctgtg tagcctaatt
      661 tacgggacgc ttcctgttgt aacgtagacg ttgtaaatgg cgcagccggt ttgcggcgac
      721 gctctccgcg tttgaccttt gaaatggaaa aagaagcagt ttcgaggtcg taaagaagcg
      781 gctctacagt ctccttgctg ggtaattccg gctctttatc atccacacgc acgagtttgg
      841 cgataaagtt cgatttcagt ttttcaggtt tgaattcagc atggatcgtc caatactcaa
      901 ccggtttgaa agcatcgatc tcgcgctcac ggtccacgat aaggcgcaaa gcgacagact
      961 gcacacgacc tgcagagagg cgcccgcgga ccttttccca caaaatgggg ctgatgctat
     1021 agcccacaag gcgatccaac acgcggcgcg cttgttgggc attgaccaag ttcatgtcaa
     1081 tattacgcgg atgcgagaaa gcatctgcaa cggcaggggc tgtaatttca tggaagacca
     1141 cgcgtttggt acgctcgggc tcaatgccag ctgcttccat cagatgccat gaaatcgact
     1201 caccttcgcg gtcgggatcg gttgcgagaa agatttcttc cgcttttttc gcaagctgtt
     1261 taatttcttt gacaacatct ttcttttcat tgggaacgcg atatttcggc tcgaaattat
     1321 tatccacatc cacagaaagc tgagacttca aaaggtcgcg cacatgcccc accgaagcac
     1381 ggacggtata gccctttcca agaaaacgcc ccaccgtttt tgccttggca ggcgactcaa
     1441 caattacaag tttgccttct cgcacttcca ccttttcagg cttgggcggc ggaatcaaac
     1501 cagcatgagc atctgtcttg cccatgcgaa ataattttgt gccacaaacc gggcaagtcc
     1561 ctgttgtcac ggcagatgac ttggcattga aacctgccac aggatcttgc atttcccgtt
     1621 tggtcttgca cttcatacaa taagcttcca tttgtattcc tccaacatat ctctcacaaa
     1681 gcagaatttg cctcgagagt tgttcggttc gtataaaaaa catcccgcaa ggttaaacct
     1741 tacgggatgg acattctaca acaagtttcc gcttttttgc aaacgccaat ttttcggg
//
LOCUS       DAOQFT010000530         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_46844_length_1794_cov_7.090282, whole genome shotgun sequence.
ACCESSION   DAOQFT010000530 DAOQFT010000000
VERSION     DAOQFT010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46844_length_1794_cov_7.090282"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..>1794
                     /locus_tag="PLR93_11680"
     CDS             27..>1794
                     /locus_tag="PLR93_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNH27676.1"
                     /translation="MIAVVALASFGMLISPLSLNPNQQTYDIGEVTQSSIRSPRDIEY
                     VSEIRTEEARKAAESAVQSVYSPPDPSITRAQIERLRTSLLNISAIRDNKELTNEEMR
                     QSLLSLSDLRLKDETIDYLLGISSTRWQSVQDEAVRVLEQVMRRAIYEDRVEAAQSSI
                     SSSVSLTFNEQQTELVTELVSPFVIANSFFSQELTDAEKLRAREAIQPIVQTYKAGET
                     IVPAGEIITPADVEAFQQLGMIRPGQRWEDIAATAAIVLLCAAFVPIYFYRKPRSVFL
                     NDPKNLTIISLLFVVFLAGARLLAERTLAPYGYPIQAAGLLLTALFSMEVGLVFAIPL
                     AVLSAFGLPNSADLIPYYMFTSLIGLLALGPARRFWGFLRAGIAISISGAAVLMAFRI
                     PLITLDWLGILQYMGMIAFAGFSSASIALLLQYIFAQILGLTTALQLLDISRPDFPLL
                     QFFLRNAPGTYQHSLQVANLAEQAAEKISADPLLTRVGALFHDIGKALNPNFFIENQV
                     QGNLNAHNNANPEEVSATIIRHVTDGVQLAKKHRLPRRLHDFILEHHGTLITHYQYNQ
                     AMEAVGGDISKVDIEKFRYPGPR"
BASE COUNT          407 a          473 c          418 g          496 t
ORIGIN      
        1 gccgtactcg aatattgcaa tacctgatga tcgccgtcgt tgcactggct tcattcggta
       61 tgctcatttc gcctttgagc ttaaacccca atcagcagac ttatgatatt ggtgaagtga
      121 ctcaatcttc gatacggtcg ccccgtgata ttgagtatgt gagtgagatc cgcacggaag
      181 aagctcgcaa agccgctgag agcgcggttc aatcggtata tagcccgcca gacccgtcca
      241 tcacccgcgc acagatcgaa cgtttgcgca cttctctttt gaatatctct gccattcgcg
      301 ataataaaga actgaccaat gaagaaatgc gtcagagttt gttatcactg agtgacttgc
      361 gtctgaaaga cgagacgatc gattacctgc tggggatttc aagtacacgt tggcaatctg
      421 ttcaagatga agccgtgcgc gttctggagc aggtgatgcg ccgtgccatt tacgaagacc
      481 gtgttgaagc ggcacaatcg agcatctctt cttctgtaag cctgaccttt aatgaacagc
      541 aaaccgaact ggtgacagaa ttggtttcgc cgtttgtgat cgcaaacagt ttcttcagcc
      601 aagaattgac cgacgctgag aagttgcgtg cacgcgaagc cattcaaccc attgtgcaaa
      661 cctacaaagc aggcgagacc atcgtcccgg caggggagat cattacaccg gcagatgtgg
      721 aagcctttca acaactaggt atgattcgcc ctggtcagcg ttgggaggat attgctgcta
      781 cggctgctat cgttttgctg tgcgccgcat ttgttcctat ttatttttat cgcaaaccgc
      841 gttccgtatt cctgaacgac ccgaagaatc tcacgatcat ctccctgctc tttgtggtct
      901 ttttagcagg ggctcgtctg ttagcagaac ggactcttgc gccctatggc tatccaatcc
      961 aagcggctgg gttattgctc actgccttat ttagcatgga agtgggcttg gtctttgcca
     1021 ttccgttggc ggtcttatct gcatttggtt tgcccaactc tgccgatctt attccctatt
     1081 acatgttcac atcgctgatc ggactgctgg cgttgggtcc ggcacgccgt ttctggggct
     1141 tcttgcgagc aggcattgcc atttctattt caggtgccgc ggtgctgatg gcatttcgca
     1201 ttccgctcat cacattggat tggcttggca ttcttcaata catgggcatg atcgcctttg
     1261 caggcttctc ttctgcgagc atcgcgcttt tattgcaata catctttgcg caaatcctcg
     1321 ggctgaccac tgccttgcaa ttactagata tttcacgccc cgactttccg cttttacaat
     1381 ttttcttacg caatgcgccc ggcacctatc aacatagttt gcaggttgct aatttggcag
     1441 agcaagccgc cgaaaaaatc agtgcagacc ctttgctcac tcgcgtaggc gctttatttc
     1501 acgatattgg taaggcgctc aacccgaact tctttattga gaatcaagtg cagggcaatc
     1561 tcaacgcaca taataatgct aaccccgaag aagtttctgc caccatcatt cggcatgtta
     1621 ctgatggtgt gcagcttgcc aagaaacatc gccttccgcg ccgcttgcat gatttcatcc
     1681 ttgaacatca tggcacgctc atcacacact atcaatacaa ccaagccatg gaagccgtag
     1741 gtggcgacat ctctaaagtg gacattgaaa aattccgtta tcccggtcca cgcc
//
LOCUS       DAOQFT010000531         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_47029_length_1790_cov_5.819020, whole genome shotgun sequence.
ACCESSION   DAOQFT010000531 DAOQFT010000000
VERSION     DAOQFT010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47029_length_1790_cov_5.819020"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1790
                     /gene="nifJ"
                     /locus_tag="PLR93_11685"
     CDS             145..>1790
                     /gene="nifJ"
                     /locus_tag="PLR93_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNH27677.1"
                     /translation="MNKNIIMIDGNEATASVAHRLNEVIAIYPITPSSAMGEFSDEWS
                     AKGKKNIWGTVPLVVEMQSEGGAAGTVHGALQTGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVAARAIAAHALSIYGDHQDVMAVRQTGWAMLSSGSVQEAQDFAAISQAAT
                     LKARVPFLHFFDGFRTSHEVNKIIQLSDEELRSMLDDDLIRAHRARGLSPEHPVLRGT
                     AQNPDVYFQMREAANPYYAAVPAITQEMMDKFAKITGRHYNLFDYSGDPNAERVIIIM
                     GSGGEVVEETVNYLAQKGEKVGVLQIRLYRPFSVEHFIKALPKSVKSLAVLDRTKEPG
                     AMGEPLYMDVVSALVEGQRSNIKVIGGRYGLSSKEFTPAMAKAVFDELTKPEPKNHFT
                     VGINDDVSNTSLTVDSSFSIENEGMVSCVFFGLGSDGTVGANKNSIKIIGEETDNFAQ
                     GYFYYDSKKSGTITMSHLRFGPKAIRAPYLIEASQANFVACHQYSFLERVDMLKYAKE
                     GGIFLLNSLYGPEEIWDQLQQEVQKDIIEKKLKFYVINGY"
BASE COUNT          479 a          484 c          402 g          425 t
ORIGIN      
        1 ttttatttcc accaatccta tccaatatat gacatcaggc agggggattt aagtcatata
       61 taactaacgt atttcatccc gcaggaataa acttattcat tggaaaatac gaggcattcg
      121 tgcctttttt acaggagcaa aagcatgaat aagaatatca ttatgatcga cggaaacgaa
      181 gccacagcca gtgtagccca ccgtctcaat gaagtgatcg ccatttaccc gatcacccca
      241 tcctctgcga tgggtgaatt ctcagacgaa tggtcagcta agggcaaaaa gaacatttgg
      301 ggaaccgttc cactcgtggt tgaaatgcaa tctgaaggcg gcgcagccgg aaccgtacac
      361 ggcgccctcc aaaccggtgc tcttaccacc accttcaccg cgtctcaagg cttattgttg
      421 atgatcccca acatgtacaa aattgcgggc gaactcacca gcaccgtctt ccacgtggca
      481 gcgcgcgcca ttgcagccca cgctctttcc atttatggcg accatcaaga tgttatggct
      541 gtccgccaaa ccggttgggc aatgctctcg tctggtagcg tgcaagaagc acaagatttt
      601 gccgcaatct ctcaagccgc caccctcaag gcacgcgtgc ccttccttca tttctttgat
      661 ggatttagaa cctcgcatga agtgaacaag atcatccaat tatcggatga agagctccgc
      721 agcatgctgg acgatgacct gattcgagcc catcgagccc gcggactcag ccctgagcat
      781 cccgtcttac gcggtactgc ccagaacccc gatgtctact tccaaatgcg tgaagcggca
      841 aacccttact acgctgccgt ccctgccatc acgcaagaaa tgatggataa gttcgccaag
      901 atcactgggc gtcactataa cctgttcgat tactccggcg acccaaatgc cgaacgtgtc
      961 attatcatca tgggttctgg cggcgaagta gtcgaagaga ccgtcaatta tcttgcgcaa
     1021 aaaggcgaaa aggtcggtgt cttgcaaatt cgcttatacc gcccattctc tgtggagcac
     1081 ttcattaaag cgttgcccaa gagcgtgaaa tctctcgccg tgttagaccg caccaaagaa
     1141 cctggcgcca tgggcgaacc cctctacatg gacgtggtca gtgccttggt agaaggtcaa
     1201 cgatccaata tcaaagtgat tggtggtcgg tatgggttgt cttccaaaga gtttactccc
     1261 gccatggcaa aggcagtttt tgacgaactt accaagcccg aacccaagaa tcatttcacc
     1321 gtcggtatca atgacgatgt ctccaacact tcactcacag tagattcctc cttctcaatc
     1381 gaaaatgaag gtatggtcag ttgtgtcttc ttcggtctcg gctcagacgg aactgtaggt
     1441 gcaaacaaga actccatcaa gatcatcggt gaagagacgg ataactttgc ccaaggctac
     1501 ttctattatg actcgaagaa atcgggcacg atcactatgt cacacttgcg ctttggtccc
     1561 aaagccattc gtgcgccgta tctgatcgaa gccagccagg caaactttgt agcttgccat
     1621 caatactcct tcctcgaacg cgtagatatg ctcaagtatg ccaaagaagg cggcatcttc
     1681 ctactcaaca gtctttatgg tcccgaagag atctgggatc agctccaaca ggaagtacaa
     1741 aaagacatta ttgagaagaa gctcaagttc tacgttatca acggctatga
//
LOCUS       DAOQFT010000532         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_47126_length_1788_cov_4.025967, whole genome shotgun sequence.
ACCESSION   DAOQFT010000532 DAOQFT010000000
VERSION     DAOQFT010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47126_length_1788_cov_4.025967"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /gene="rimO"
                     /locus_tag="PLR93_11690"
     CDS             <1..1121
                     /gene="rimO"
                     /locus_tag="PLR93_11690"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNH27678.1"
                     /translation="AGCLTQRYGVEVAKRVPGIDGIIGTRRWMDIVQVVEDARKGKHP
                     EPIYHLPEAATVGSDEHGAMRVSVTGPSAYLKIADGCRRPCAFCAIPLIKGTAVSRPV
                     ESIIHEARTLRDNGVRELILIAQDSTDYGHDLGMKNGLATMLEQLTDAVPDMDWIRIM
                     YSYPGYVTDRMIEVMATRKQILPYLDIPLQHAHPKTLYRMKRPSNMDWVHKTLAKMRS
                     TIKDLAIRTTFIVGYPGETDEEFQALYDFVEQTRFDRAGAFQFSFEPGTTSAPLGDPV
                     PAEVKQERYERLMELQQNISLQVNQSYVGKTLDVLVEGFDNGISVGRSYRDAPEVDGY
                     VVVEGKAKVGDIVPVRITGAFAYDLTGVLVEQQNLIKL"
     gene            complement(1172..1678)
                     /locus_tag="PLR93_11695"
     CDS             complement(1172..1678)
                     /locus_tag="PLR93_11695"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNH27679.1"
                     /translation="MDTPSTPVLAFIYWMHMLATVTWIGSLAAINLLFLPASTRTLKL
                     PDQLSLITALQKRLEPLAWFCMGILVVTGLFQMSTSPHYDGFLSISTQWSLSILIKHG
                     FAVIMVVVSAIQTWEVLPAIQRTLLKKDKADEAELSRLQKRETLLLRLNLLLSALILG
                     ATAFARVS"
BASE COUNT          428 a          401 c          499 g          460 t
ORIGIN      
        1 cggctggttg tttaactcag cgttacggtg tggaagtggc gaagagagtg ccgggtattg
       61 acggcattat cggtacacgt cgctggatgg atattgtgca ggtggtggaa gatgctcgca
      121 aaggcaaaca cccggagcct atttatcact tgccagaagc cgctacggtc ggttccgatg
      181 aacacggtgc catgcgcgtc agcgtgactg gacccagtgc ctatctcaaa attgcggatg
      241 gttgccgccg tccgtgtgcg ttttgtgcga ttccacttat taagggcact gcggtttccc
      301 gtccggttga gtcgattatc catgaagcac gcaccttgcg cgataacggt gttcgcgaac
      361 tcattttgat tgcacaagat tcaaccgatt atggtcatga ccttggtatg aaaaatggtc
      421 ttgcgaccat gcttgaacag ttgaccgatg ccgtgccgga tatggattgg atccgcatca
      481 tgtattcgta tccaggctat gtcacagatc gcatgatcga agtaatggca actcgcaaac
      541 agatcttgcc gtatcttgac atcccgctgc aacatgccca tcccaaaact ttgtatcgca
      601 tgaagcgccc atcgaatatg gattgggtcc ataagacgct tgccaagatg cgttcaacca
      661 tcaaagacct tgccattcga acaacattca ttgttggata tcccggcgaa accgatgaag
      721 aatttcaagc cttgtacgat ttcgtggagc agacccgctt cgaccgtgcc ggagcttttc
      781 aattctcctt cgagccgggt actacctccg cgcctttagg tgacccagtc cctgctgaag
      841 tgaagcagga gcgttatgag cggttgatgg aattgcagca gaatatttcg ttgcaagtca
      901 atcaatctta tgtaggcaaa acgcttgatg tgttggtgga agggtttgat aacggcatct
      961 ctgtgggacg ctcttaccgc gatgcacccg aagtagatgg atatgtagtt gtggaaggca
     1021 aagcaaaggt gggggatatt gtccccgtac gaatcaccgg tgcctttgcc tatgatctga
     1081 ccggcgtgct ggtagaacag cagaatttga tcaagctgta attgttatta aaataaaaat
     1141 cggaagtcgt tacgacttcc gattttttga ttcaagatac tcttgcgaat gcagttgctc
     1201 cgagaatgag agcggagagc aacaagttga gccgtaacaa aagcgtttcg cgtttttgca
     1261 gacgggaaag ttcggcttca tctgctttgt ctttctttag tagagtccgc tgaatggcag
     1321 ggagcacttc ccaggtttgg atggcgctga cgacaaccat gatgacggca aatccatgct
     1381 tgatgagaat ggatagtgac cattgcgtgc tgattgaaag aaagccatcg taatgtgggc
     1441 tggtgctcat ttggaataga ccggtcacca caaggatgcc catacagaac catgcgagag
     1501 gttccagtcg cttttgcagg gcggtgataa ggctaagttg gtcgggcagt ttcaaggtcc
     1561 gcgtggaggc gggtaggaag agcaaattaa tggctgccag acttccgatc cacgtgacgg
     1621 ttgccagcat gtgcatccag tagatgaacg cgagaactgg tgttgagggt gtatccatgt
     1681 ttatggcgta gctgtaggtt caacaacggg agcttctgtt gctgcaggtt cggcggggta
     1741 acatgccgca tacgcctgtg ctgagtttgc tgaggcttct ccatctaa
//
LOCUS       DAOQFT010000533         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_47252_length_1785_cov_5.445665, whole genome shotgun sequence.
ACCESSION   DAOQFT010000533 DAOQFT010000000
VERSION     DAOQFT010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47252_length_1785_cov_5.445665"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1103
                     /locus_tag="PLR93_11700"
     CDS             204..1103
                     /locus_tag="PLR93_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNH27680.1"
                     /translation="MTKNSAPAHISAKTLLPAAINGWEMFLLDQGRSPNTVKAFLSDV
                     RLLTQHLPPDLSIGAVTTDDLNRFFEWMEKGRGVACSPKTLSRRITSVKSFFRWLHQY
                     GTLSIDPAEKVLQRSAISPLPQVLTDEEYEAVLLAADRQRRAKKPDARPYTLLSLLLQ
                     TAIKKSECLGIHVNHIELAGKNGPFLFVRYASPANRYKERKIELTEDWVEAYNEYLTQ
                     YSPVDQAFPWSPRRLEYLLEDISEEAGLEKHLSFDMCRWTSALRDYQNGIEPDKIRQK
                     LGVSKIQWRELFIKLKQLNGEVK"
     gene            complement(1123..>1785)
                     /gene="tilS"
                     /locus_tag="PLR93_11705"
     CDS             complement(1123..>1785)
                     /gene="tilS"
                     /locus_tag="PLR93_11705"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNH27681.1"
                     /translation="LQRNLIRHAMQTLLPGVDIGYQTLKRASDFINASSATVPRLDLK
                     AGLRMFREAGLIHICAPYAELPFDRWPQMVDDKPVPLRLGGQASLAGGWKVICESWRL
                     PTLAREQAEENEDQFQVWLDADELPEEFLLRARRQGDHFSPLGMGGHSQKLSDFFVNE
                     KLPHRARDRWPLLCVGDEIIWVPGFRPAHRYRLKETTHNVIYFSVQHPPEKIVPDENP
                     HP"
BASE COUNT          496 a          474 c          403 g          412 t
ORIGIN      
        1 aagcctgccg atcatccaga agatagccaa agctatggcg ggagaaaaag taaacccttg
       61 gagttacttg cctccaccat aagatgtgaa gttaacaacc tccgagatga aaatttcgga
      121 ggtttatttt ttgccagttg acaacgttcc tcaaattcgc taaactatac gcaagaataa
      181 atactacacg caaggtaaac cgtatgacga aaaattctgc accagcccat atcagcgcca
      241 aaacgctgtt acccgccgca atcaacggtt gggaaatgtt cctcctcgat cagggacgtt
      301 cccccaacac cgtcaaagcc ttcctttcgg atgtacggct tctcactcaa catttgccgc
      361 ccgatctgtc tatcggcgca gtcactaccg atgacctcaa ccgcttcttc gagtggatgg
      421 aaaagggtcg tggcgtggca tgcagcccca aaaccttgtc gcgtcgtatc acatcggtta
      481 agtctttctt ccgctggctt catcaatacg gcacgttaag cattgaccct gccgaaaaag
      541 ttctgcaacg ctctgccatt agcccgttgc cacaggttct taccgacgaa gaatatgaag
      601 cggtgctttt ggcagcagac cgtcagcgcc gcgccaagaa gccagatgcc cgcccataca
      661 ccttgttgtc tctcttgctt caaactgcta tcaagaaaag tgaatgcctt ggcattcatg
      721 tcaatcacat tgagttggct ggtaagaacg gtcccttcct ttttgtgcgc tatgccagcc
      781 ctgccaaccg ctacaaagaa cgcaaaattg aactgaccga ggattgggtt gaagcctaca
      841 acgaatacct cacacaatac agccctgtag accaagcttt cccctggtca ccgcgccgtt
      901 tggaatatct gcttgaagat attagcgaag aagccggttt ggaaaaacat ctctcctttg
      961 atatgtgtcg ctggacatct gccttacgag attatcagaa tggcattgaa ccagataaga
     1021 tccgccaaaa actaggcgta tcaaaaatcc agtggcgcga gttattcatc aaactcaagc
     1081 aattaaacgg cgaagtcaaa taaaataaaa aatccccatc cactacggat ggggattttc
     1141 atctgggaca atcttctcgg gcggatgttg aaccgaaaaa taaatcacat tatgggtcgt
     1201 ctctttcaaa cggtagcgat gtgccgggcg aaagcctgga acccaaatga tttcatcacc
     1261 cacacacaag agaggccaac ggtcacgggc acgatgaggc agtttctcgt tgacaaaaaa
     1321 gtctgaaagt ttttgagaat gaccgcccat tcccagcgga gaaaaatgat cgccctgacg
     1381 acgagcccgc aatagaaatt cttcaggaag ttcatccgca tccaaccaga cctgaaactg
     1441 gtcctcgttt tcttcggctt gttctctcgc caatgttggc agacgccaac tttcacagat
     1501 caccttccac ccaccagcta gtgaagcctg cccgcctagt cgtaatggaa ccggtttgtc
     1561 gtccaccatt tggggccaac ggtcaaaggg aagctcagca taaggcgcgc agatatgaat
     1621 cagcccggct tcgcggaaca tacgtagacc tgcttttaag tccaaacgcg gaaccgtagc
     1681 actggaagca ttaataaaat cggaagcgcg tttcaaggtt tggtatccaa tgtccacgcc
     1741 tgggagaaga gtctgcatgg catggcgaat caggttacgt tggag
//
LOCUS       DAOQFT010000534         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_47304_length_1784_cov_6.304800, whole genome shotgun sequence.
ACCESSION   DAOQFT010000534 DAOQFT010000000
VERSION     DAOQFT010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47304_length_1784_cov_6.304800"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /gene="wecB"
                     /locus_tag="PLR93_11710"
     CDS             complement(<1..650)
                     /gene="wecB"
                     /locus_tag="PLR93_11710"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNH27682.1"
                     /translation="MRILTVFGTRPEAVKMAPIVKLLASTPGVEARVCVTAQHRQMLD
                     QVLNLFQIEPHYDLNLMRDGQSLAQLSADIFTHLDPVLEDFKPDWVLGQGDTTTVAIT
                     SMLCFYRQIRFGHVEAGLRTHDKWQPFPEEVNRRIVSVIADLHFAPTNWSKNNLLREG
                     VDEKTVRVTGNTVIDALQFVAKQAEPAEITELVQSKLSHKKLILITAHRRENFGAP"
     gene            699..854
                     /locus_tag="PLR93_11715"
     CDS             699..854
                     /locus_tag="PLR93_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27683.1"
                     /translation="MFKLIKFTKRSIILALYFSLILGLASCAKATCGMQAVAGGSQIC
                     IEVLNAA"
     gene            complement(855..>1784)
                     /locus_tag="PLR93_11720"
     CDS             complement(855..>1784)
                     /locus_tag="PLR93_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNH27684.1"
                     /translation="ESTSPPRTTFDLVAPILEKSKLKAGVDFHLCYSPERVLPGQILR
                     ELIENSRVIGGVTPESAKAGADLYSTFVKGQIIQTDATTAEMVKLMENTYRDVNIAIA
                     NEFSRLADKFGVDVWEAISIANLHPRVKILSPGPGVGGHCISVDPWFFVEAAPNLTPL
                     IYNSRQVNDAQPAFVVDVVKRALGSLKGKKIAALGLAYKPDVDDLRESPAVEAVHFLQ
                     KEGAEVLACEPFKPDAKLPSVTIAPTFEDAVRDADAIILLVKHTEFSKLKPEQVAQQT
                     KARLLIDTVQGWDAEQWKHAGFRHVKLGSGKPA"
BASE COUNT          437 a          418 c          452 g          477 t
ORIGIN      
        1 atcggcgcgc caaagttctc acggcgatgc gctgtgatga gaattaactt tttatggctc
       61 agtttagatt gaaccagttc cgtgatttct gctggttcgg cttgttttgc cacgaactgc
      121 aaagcatcaa tgactgtgtt cccggttact ctaacggttt tctcgtccac accttcgcgc
      181 aataaattgt tctttgacca attcgtgggc gcaaaatgca aatccgcgat gacgctgaca
      241 atgcggcggt ttacttcctc tgggaaaggc tgccacttgt catgggtccg caaaccggct
      301 tccacatgcc cgaagcgaat ttggcgataa aagcacaaca tggatgtgat ggcgacggtg
      361 gtggtatcgc cttgtccaag cacccagtct ggtttgaaat cttctagaac agggtcgaga
      421 tgcgtgaaaa tatccgcact taattgggca agagattgtc catctcgcat aaggttcaaa
      481 tcataatgtg gttcaatttg gaacaggttc aacacttggt caagcatctg gcggtgttgc
      541 gccgtaacac aaacccgagc ctcaacacct ggcgtagaag caagcagctt cacaattggt
      601 gccattttga cggcttcggg tcgagttcca aaaactgtaa ggattctcat aagtcctttt
      661 tgggcctatt tttctactag gtgtgtggag aaacaaaaat gttcaaatta attaagttta
      721 caaaacgttc tataattttg gcgctttatt tcagccttat tctagggctt gcatcttgtg
      781 caaaagccac ctgcggtatg caagcggttg caggtggctc gcaaatatgt atcgaagttc
      841 tgaacgctgc gtaactatgc gggctttcca gacccaagct tgacgtggcg gaaacctgca
      901 tgcttccatt gctctgcatc ccaaccttgg acggtatcaa tcaagaggcg tgcttttgtt
      961 tgttgagcaa cttgttcagg cttgagcttg ctaaattcgg tatgttttac gagcaagata
     1021 atcgcgtcgg catcacgaac tgcatcttca aaggttggcg caattgttac gctgggcaat
     1081 ttggcatctg gtttgaaagg ctcgcaagcg agaacttctg cgccttcttt ttggagaaag
     1141 tgcaccgctt caacagccgg gctttcacgt aaatcatcca catcaggctt gtatgccaag
     1201 ccgagagcgg cgatcttttt ccctttgagg gaaccaagtg cgcgtttgac cacatctaca
     1261 acaaaggctg gttgcgcatc gttgacttgg cgagagttgt aaatcagcgg agtgagattt
     1321 ggcgccgcct ctacaaagaa ccaagggtct acgctgatac agtgaccacc aacccctggt
     1381 cccgggctga gaattttgac acgtgggtgc aaattggcaa tggagatggc ttcccagaca
     1441 tccacaccga atttatcggc aaggcgagag aattcattag caatggcaat gttgacatcg
     1501 cgataagtat tctccatcag tttgaccatt tcggctgtgg tcgcatcggt ttgaatgatc
     1561 tgccctttga cgaaggtaga gtacaagtct gctccggctt tagcagattc aggggtcacg
     1621 ccgccgataa cgcgtgagtt ttcaatcaac tcgcgcagaa tttgccccgg caagacgcgt
     1681 tctggtgaat agcaaaggtg aaagtccacg cccgctttca gcttggattt ttctaaaatg
     1741 ggggcaacca agtcaaaggt cgtgcgaggc ggagaagtcg attc
//
LOCUS       DAOQFT010000535         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_47828_length_1773_cov_4.694994, whole genome shotgun sequence.
ACCESSION   DAOQFT010000535 DAOQFT010000000
VERSION     DAOQFT010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47828_length_1773_cov_4.694994"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLR93_11725"
     CDS             <1..262
                     /locus_tag="PLR93_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNH27685.1"
                     /translation="GGIQEEAPAFGIPTLVLRDVTERPEGVDAGTLKLVGTETSHIVE
                     ETRRLLEDESAYKKMATSANPYGDGHAAEKIIQALLGSRENM"
     gene            332..1270
                     /locus_tag="PLR93_11730"
     CDS             332..1270
                     /locus_tag="PLR93_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27686.1"
                     /translation="MRKTFTPIVLTLLLLAAWIPVHAQSVETQYFSETGHNVSGDFLA
                     FYNSNPNAAYLYGYPITEQITGRDGKSVQYFQRARFEFRADLPEGQRVQLTQLGRETY
                     ISTGLLDVGNSFACRSYTETGFSVCFAFLEFFDANGGVAQFGYPISGFEYHENKIVQY
                     FERARLEWQPWRADGYRVVISDLGRAYFDKLGEDPALLAPASPLNNAPRVITELRIRA
                     FVWKAVTLSNDEQMFFVIVQDQNAQPVPGASCTAVVNWPNGAKDSTIITSDTNGVGVV
                     SLAFQNQPYGSLIYTDINCTYNNLTGKTTTSFRIWY"
     gene            complement(1300..>1773)
                     /locus_tag="PLR93_11735"
     CDS             complement(1300..>1773)
                     /locus_tag="PLR93_11735"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27687.1"
                     /translation="SWQPGQLLHLRGPIGHGFNLPLSARKIALVAFDDIPAHLLGLLS
                     QALKQNAEVVLVSNSSVEDLPEVVEIQPVKGLLDVLRWADYVAMDVNREKLNQLKEML
                     AGKDQITAKVEAQVLIHAPMPCGALADCGVCALSIDHDWKMICKDGPVFELKEIL"
BASE COUNT          458 a          458 c          410 g          447 t
ORIGIN      
        1 aggcgggatt caggaagaag ccccagcctt tggcatccca acactggtac tccgcgatgt
       61 gaccgaacgc cctgagggag tcgatgcggg gacattaaag ctggttggaa ccgaaaccag
      121 ccacatcgtc gaagaaacga ggcgcctctt agaggatgaa tctgcataca aaaagatggc
      181 aacatccgcc aatccatacg gagatggaca cgccgcagag aaaatcatcc aagctttact
      241 aggcagtagg gaaaacatgt aatctttctc aagataactg tttgtaatgt cttgaggggt
      301 gtttctgagt ataatgacag tggtggtagt aatgaggaaa actttcacgc ccattgttct
      361 gacgcttctg ctcctggctg cttggattcc tgttcatgca cagtctgtag agactcaata
      421 tttcagtgag acgggacata atgtctctgg agactttctt gctttttaca atagcaaccc
      481 taacgccgcc tacctttacg gataccccat aaccgaacaa attaccggtc gcgatggaaa
      541 aagtgtgcaa tatttccaac gtgcgcgttt tgaattccgt gccgacctgc ccgaagggca
      601 acgtgttcaa ttaacgcaac ttgggcgcga aacctacatc tccaccggtt tgctggatgt
      661 tggcaattca ttcgcttgtc gcagctatac cgaaacaggc ttctcggtct gttttgcttt
      721 tcttgagttt ttcgacgcca atggcggtgt agctcagttc gggtacccaa tctctggttt
      781 tgaataccac gagaataaga tcgtccagta cttcgaacgc gcgcgccttg aatggcagcc
      841 ttggcgagcc gacgggtatc gtgtggtaat ttcagatttg ggtcgtgcct acttcgataa
      901 gcttggtgaa gaccctgccc tgcttgcccc tgccagcccg ctcaacaatg ctccacgcgt
      961 tatcacagaa ttgcgaattc gcgcctttgt ctggaaagcc gttaccctct cgaatgacga
     1021 gcagatgttc ttcgtgatcg tacaagacca aaacgctcag ccagttcccg gcgcatcttg
     1081 tacggcggtg gtcaactggc ccaatggcgc aaaagattca acaatcatta caagtgatac
     1141 aaatggcgtg ggcgtcgtct cgctcgcttt tcagaatcaa ccttatggta gtttgatcta
     1201 cacagacatc aattgcacct acaacaacct aaccggcaaa accacaacat ccttcagaat
     1261 ttggtactaa gcaaaagcct cccaaatggg aggctttttt tataagattt ctttcagctc
     1321 aaacacagga ccatctttac aaatcatttt ccaatcatga tcaatggata aagcgcagac
     1381 gccgcaatct gccagtgcgc cgcaaggcat aggagcgtgg ataagaacct gcgcctccac
     1441 cttcgccgtg atttggtctt tccctgccag catttctttt agctggttca gcttctccct
     1501 atttacatcc atggcaacat agtctgccca gcgaagcacg tcaagtagcc ctttaaccgg
     1561 ctgaatttct accacctcgg gcaagtcttc tacagacgag ttggaaacca ataccacttc
     1621 ggcattttgt ttcaatgctt gtgaaagcaa gcccaataaa tgggcaggaa tatcatcaaa
     1681 agcgacaagc gctattttcc tcgcagagag tggtaagttg aaaccatgcc cgatcggtcc
     1741 gcgtagatgt agtaactgac ctggctgcca gga
//
LOCUS       DAOQFT010000536         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_47881_length_1772_cov_4.125801, whole genome shotgun sequence.
ACCESSION   DAOQFT010000536 DAOQFT010000000
VERSION     DAOQFT010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47881_length_1772_cov_4.125801"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLR93_11740"
     CDS             <1..645
                     /locus_tag="PLR93_11740"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27688.1"
                     /translation="IGSGNDFAYSIGVKQRSAQALAHTLRAETVKGVDIGLMTDEHGR
                     REYFDNTLGIGFDAVVTIRSHKLPIVKGFLMYLTAVIQTIILNHNPARIKAEADSGNW
                     EDQLLMLTLCNGPREGGGFMLAPEAKNNDGILSIVGVKKVSRAKMFRLVPEFMNGTHM
                     RFKEIRMGECKKLSLTSDIPLYIHADGEIFTSFGSNLKKATFEILPQAIKVVHG"
     gene            complement(702..1208)
                     /locus_tag="PLR93_11745"
     CDS             complement(702..1208)
                     /locus_tag="PLR93_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27689.1"
                     /translation="MTLVFSILRILHIVTGILWAGGALALTLFVAPTMSATGEAGKQF
                     AGHLITKTRFSLYMMVTGLSTVLAGSVLYGIDSNWFQSAWMMTGMGIGFGIGAVAGIA
                     AFIFGIMLGNANSALAALGAQIQGKPTAEQMTALQGLLKRQALTSQMNMIFMFIAIVM
                     MASARLLG"
     gene            1278..>1772
                     /locus_tag="PLR93_11750"
     CDS             1278..>1772
                     /locus_tag="PLR93_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27690.1"
                     /translation="MPTLEQSLHKTDIGHLRIIAEFWGLELEANDFDAALEELCASLL
                     DLEAVSETLDILPAEARSALNLLIDAEGRIEWAVFARKYGDIREMGPGKRDRERPHLK
                     PASTSEPLFYRALLAKAIFETEKGPQEFAYIPDDLLEIINEVMIEAPGEKSSQKSEPL
                     GRPAT"
BASE COUNT          494 a          475 c          398 g          405 t
ORIGIN      
        1 attggctcag gcaacgactt cgcctattcc attggcgtta aacaaaggtc agcccaggca
       61 ttggctcata ccttgcgcgc cgaaacggta aagggcgtag atatcggctt gatgaccgat
      121 gaacatggaa gacgggaata tttcgataac acccttggca ttggctttga cgccgttgtc
      181 accattcgtt cgcacaaatt gccgattgtt aaaggctttt tgatgtatct caccgcagtc
      241 attcaaacca tcattttgaa tcacaaccct gcccggatca aagccgaggc agatagcggc
      301 aactgggaag accaattgct tatgctcact ctttgcaacg gcccgcgtga aggcggtggt
      361 ttcatgcttg ccccagaggc aaagaacaat gacggtatct tgagcattgt cggagttaaa
      421 aaggtttcgc gtgcaaagat gtttcgactt gttcctgaat ttatgaatgg cactcatatg
      481 cgtttcaaag agattcgcat gggtgaatgt aaaaaacttt cgctcacctc ggatattccg
      541 ctttatatcc atgctgacgg ggaaatcttc accagttttg gcagcaacct gaaaaaagcg
      601 actttcgaaa ttttgcctca ggcaattaaa gtagtacacg gataaacaaa aacagcggct
      661 gaaaatcagc cgctgtggat tataaaaaaa tcacatccga ttcaacccaa tagacgcgcc
      721 gaagccatca tgacaatggc gatgaacata aagatcatat tcatctgcga cgtcagcgct
      781 tgtcgtttca aaagtccttg caaggcagtc atttgttctg ccgtgggttt cccttgaatc
      841 tgcgcgccca aggcagccaa tgcactattt gcattgccaa gcatgattcc aaagataaac
      901 gccgcaatac cagccactgc cccaatgcca aagccaatac ccatgccagt catcatccat
      961 gcagattgga accagtttga atcgattccg tataagaccg aacccgccaa gaccgtactc
     1021 aaccccgtaa ccatcatgta caggctaaaa cgcgtcttcg ttatcagatg accagcaaac
     1081 tgtttgcctg cctccccggt ggcactcatg gtcggcgcta caaataaagt caacgccagg
     1141 gcgccgccag cccaaagaat gcccgttaca atgtgaagaa tccgcaaaat cgaaaatact
     1201 aaagtcatgg ttgatctcct ttgactgata aaaccctgaa ttccaaaatc gctacgtagt
     1261 gatgttagaa tccggccatg cccacccttg agcaaagcct gcacaaaaca gatattggac
     1321 atcttcgcat cattgccgaa ttttggggat tggaattgga agccaacgac tttgatgccg
     1381 cactcgaaga attatgcgcc tccctgctgg acctggaagc tgtctctgaa accctggaca
     1441 ttctccccgc cgaagcccgc tcggcattga atctcctgat cgacgcagaa ggccgcatcg
     1501 agtgggccgt cttcgcccgc aaatacggag atattcgtga aatggggcca ggcaaacgcg
     1561 atcgtgaacg accgcatctc aaacccgcat ctacatccga gccgcttttc tatcgcgccc
     1621 tgctggccaa agccatcttc gaaaccgaga aaggcccgca agaattcgcc tacattccag
     1681 acgatctgct cgagatcatc aacgaggtta tgatcgaagc acccggtgaa aagagttctc
     1741 aaaaatctga accgttgggt cggccagcca cg
//
LOCUS       DAOQFT010000537         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_48568_length_1758_cov_6.708162, whole genome shotgun sequence.
ACCESSION   DAOQFT010000537 DAOQFT010000000
VERSION     DAOQFT010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48568_length_1758_cov_6.708162"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLR93_11755"
     CDS             complement(<1..426)
                     /locus_tag="PLR93_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH27691.1"
                     /translation="MNFNNFNLDPRLKQGIMKAGYETPTPIQEAAIPAAIRGRDIIGT
                     AQTGTGKTAAFVLPILNKLLNGQRNTPRALIVTPTRELAEQINDVIKTLSVGTKLKSA
                     TIYGGVGANPQIQALRNGAEILIACPGRLLDLIAQGHAKM"
     gene            complement(497..1363)
                     /locus_tag="PLR93_11760"
     CDS             complement(497..1363)
                     /locus_tag="PLR93_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNH27692.1"
                     /translation="MRRLTGIILIAISAASFGTLAIFGRYAYAENIDTFTLLFLRFGF
                     SASFMTMMLILRKEPFPRGRILAQLIGMGGIGYAGQSFLYLTAIKYASAGLVALLLYL
                     YPFFVAILSMIFLQEKLTRVKAFALALALCGTALTVGPVSGQPIGAIMAIVAALGYSI
                     YIIVGANVMKHVSPFQSSTVIFASASVVYGLLTFTNGIHLPQTNFGWLVIAGIVVVST
                     IIPVTTFLAGLERIGPTNAAMLSTIEPIITVFLATVLFGDKLAPIVLAGGALILIAVV
                     ILTRSEMRSIET"
     gene            complement(1450..>1758)
                     /locus_tag="PLR93_11765"
     CDS             complement(1450..>1758)
                     /locus_tag="PLR93_11765"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNH27693.1"
                     /translation="VSPLDVLGYMEEDEFHLRKAVAQYILAQVGKPYNLNFFNSETEE
                     AFYCSQLAYKAYQTIGINLNTGLAMEQLPGTNQIVYPQEIWNGFSHHKINLFRAAVSA
                     "
BASE COUNT          472 a          387 c          463 g          436 t
ORIGIN      
        1 catctttgcg tggccctgtg cgatgagatc aagcagacgc cctgggcaag cgatgaggat
       61 ttctgcgccg ttgcgcaatg cctgaatttg tgggttcgct ccgactccgc cgtaaatggt
      121 tgcgctcttc agctttgtcc caactgacag tgttttgatc acatcgttaa tttgctctgc
      181 aagttcgcgt gttggtgtga cgattaacgc acgcggcgtg ttacgttgac cattgagcag
      241 tttgttgaga ataggcaata caaaggcagc ggttttgcca gtgccggttt gtgccgtacc
      301 gataatgtcg cgtccacgaa tggcggctgg gatggcagct tcttgaatgg gggtgggggt
      361 ctcatacccc gccttcataa ttccctgctt gaggcggggg tcgagattaa aattgttgaa
      421 gttcatgatt tcctttttag gtgacagtca ctatacgtga tcgtcccctg attctttgta
      481 tgaagcatta tatcgcttat gtttcgattg aacgcatttc tgagcgtgta agaatcacaa
      541 ctgcaatgag aatcagtgct ccgccagcca atacgatcgg tgcaagtttg tcgccaaata
      601 agaccgttgc gagaaaaacc gtgatgatgg gttcaatagt cgagagcatt gccgcattgg
      661 taggaccgat ccgttccaaa ccagcaagaa aagttgtgac tgggataatg gtggacacga
      721 caacaatgcc cgcaattacc agccaaccga agttcgtctg tggaagatga attccgttgg
      781 tgaaagtcaa cagtccatag accacactcg cggaagcgaa aattacggtc gacgactgaa
      841 atgggctgac atgtttcatc acattggcac cgacgatgat gtaaatggaa taccccaatg
      901 cggcaacaat cgccataatt gcgccaatgg gctgtccact gacgggaccg accgttagtg
      961 cggtgccgca taatgccagg gcaagcgcaa aggcttttac gcgggtgagt ttctcttgca
     1021 agaagatcat cgataagatg gcaacaaaaa agggatacag atacaaaagc aaggcgacca
     1081 aaccggcaga agcatatttg atagcggtta ggtatagaaa cgattgccct gcatacccaa
     1141 tgccacccat gccgattagt tgggcgagga tgcgtccgcg tgggaaaggc tccttgcgca
     1201 gaatcaacat catggtcatg aatgaggcgg aaaatccaaa acggagaaaa aggagggtga
     1261 aggtgtctat gttttcagcg taggcatagc gcccaaagat ggcaagcgtc ccaaatgatg
     1321 cggcagaaat ggcgatcaaa atgattccgg tgagtcttct catagcgggc aactataccg
     1381 cactcaatcg taaattgtgc tttttaaatg cagtatatac ccgaaaaaaa ctccccgttg
     1441 aaggggagtt tatgctgaga ccgcggctcg aaacagattt attttgtgat gtgaaaaccc
     1501 attccaaatc tcttgcggat aaacgatttg atttgtgcca ggcagttgct ccatggcgag
     1561 acccgtgttc aagttaatgc cgatggtttg ataggctttg tatgcgagct ggctacaata
     1621 aaaagcctct tcggtttcgg agttaaagaa gtttagattg tagggcttac caacttgcgc
     1681 aaggatatat tgggcaacag ctttgcgtaa atgaaattcg tcttcttcca tatacccaag
     1741 cacatccaat ggagagac
//
LOCUS       DAOQFT010000538         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_48659_length_1756_cov_6.553792, whole genome shotgun sequence.
ACCESSION   DAOQFT010000538 DAOQFT010000000
VERSION     DAOQFT010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48659_length_1756_cov_6.553792"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLR93_11770"
     CDS             complement(<1..314)
                     /locus_tag="PLR93_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HNH27694.1"
                     /translation="MEEQNNTNQSPYAGRWVALVRGRVVAQGGTPEQALRASQSSRYK
                     ERPEIRFMSVPFNLPPLLEKIVNVLPANTEIYLVGGAVRDLLTNRRSPDFDFAVPSNG
                     ISL"
     gene            complement(336..893)
                     /gene="hpt"
                     /locus_tag="PLR93_11775"
     CDS             complement(336..893)
                     /gene="hpt"
                     /locus_tag="PLR93_11775"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNH27695.1"
                     /translation="MQNYQDFLAEVLIDTASLQKRVAELGKQISADYNGQDLLLICIL
                     RGAVPFMVDLSRHITVPHMMDFMAVSSYGIGRRESSGSARVTLDLQMDIRDHNVLLVE
                     DIVDSGHTIAAVLQMLGTRQPKSLKVCALLDKPERREAHVPIDYLGFSIPNKFVFGYG
                     LDLDEYHRNLNFVGVVDLDRYKPVQ"
     gene            complement(963..1532)
                     /locus_tag="PLR93_11780"
     CDS             complement(963..1532)
                     /locus_tag="PLR93_11780"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNH27696.1"
                     /translation="MRHTHGLIEVVCGSMFSGKTDELIRRLVRATIAKQKVQVFKPAI
                     DIRYAVEKVASHTGSTFDAIPVEKASEILSKVAADTTVVGLDEAQFFDAEIVDVAQTL
                     SDRGIRVIVAGLDMDFRGEPFGSMPVFMAKAESVLKLHAICMVCGDEASRTQRLVNGK
                     PARYDEPVVIVGASELYEARCRLHHEVPR"
BASE COUNT          471 a          439 c          423 g          423 t
ORIGIN      
        1 agagaaatgc cattcgaagg cacggcaaaa tcaaagtcgg gagagcggcg gttggtcaat
       61 agatcccgca ctgccccacc gacaagatag atttccgtgt ttgcaggaag gacattaact
      121 attttttcca ataaaggggg aaggttgaag ggaacagaca taaaacgaat ctctggtctt
      181 tctttatagc ggctggattg cgaagcacgc aaggcttgtt ctggtgtacc gccttgagca
      241 acgacacgcc cacgaacgag ggcaacccaa cgccctgcat atggtgattg attggtgttg
      301 ttttgttctt ccaagatacc ccctgcaccg ggcgcttatt gaacgggttt gtaacgatcg
      361 agatctacga cgccaacaaa atttaaattg cgatgatatt catcaaggtc gagcccataa
      421 ccgaagacga acttgttggg aatactaaag ccaaggtaat caataggcac atgcgcttcg
      481 cgacgttcgg gcttatcgag caaggcacaa acctttagcg atttgggctg gcgtgttcca
      541 agcatttgca aaacggcagc aatggtatga ccactatcca cgatatcttc aacaagcaaa
      601 acattgtggt cacgaatatc catttgaagg tctaaagtaa cacgagcaga accgctggat
      661 tcacgccttc cgattccata tgaagagaca gccatgaagt ccatcatatg tggcacggtg
      721 atgtggcggc tcaagtccac cataaaaggc accgcgccgc gcagaatgca gatgagcaac
      781 aaatcttgtc cgttatagtc ggcactgatc tgcttgccca gttcggcaac gcgtttctgt
      841 aaagaggctg tatcaataag aacttcggcg aggaaatcct ggtagttttg catcaaagtg
      901 ttccttgggt atttgagttt aattagttta tcactactaa agcccggtgt ggggcttctc
      961 cactaacgcg ggacttcgtg atgcaaacga cagcgggctt catacaactc agaagcacca
     1021 acgatgacca ccggctcatc ataacgggca ggcttgccat tcaccagacg ctgagtccta
     1081 gacgcttcat ctccacaaac catacaaatg gcatgcagct tcaacacaga ttctgccttt
     1141 gccataaaca ccggcatgga gccgaatggt tcgccacgaa aatccatatc caaaccagca
     1201 acgatgacac gaatgccacg gtctgaaagg gtctgcgcca cgtccacaat ctcggcatca
     1261 aagaactgcg cctcatccaa acccacaacg gttgtgtctg ctgcgacttt tgagagaatt
     1321 tcactggctt tttccacggg aatggcatcg aaggttgagc cggtatgcga agcgactttt
     1381 tccaccgcat aacgaatgtc gatggcgggt ttaaagacct gcaccttttg cttggcaatg
     1441 gtggcgcgta ctagtcgtcg aatcaattca tccgtcttgc cgctaaacat tgaaccgcaa
     1501 acaacttcga tcaagccgtg ggtatgcctc attgaaattc tcctgccccg ttcggggaaa
     1561 atgttttttt cataaaaaca ggcagacgct tttgcatctg cctgtttagt ttacctgata
     1621 agaagcggac tgactactca gcagcttcgg cgggcttttc ggctttttca gccttctcta
     1681 ccttgggagg ttcagggaga gtaatgtcca ttgcgcggag ctttttcttg gcagccgtca
     1741 aagcagactg accaaa
//
LOCUS       DAOQFT010000539         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_48801_length_1753_cov_6.477621, whole genome shotgun sequence.
ACCESSION   DAOQFT010000539 DAOQFT010000000
VERSION     DAOQFT010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48801_length_1753_cov_6.477621"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PLR93_11785"
     CDS             <1..1034
                     /locus_tag="PLR93_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH27697.1"
                     /translation="LDMFEEVRLASFVAKAASNDPTVLPAATALTMATRLGAQALHIG
                     HLTGSLEVGKRADLILVDTNPLHNTPRFKRDPQNAYAQLVYASKSTDVTDVMVNGKWL
                     MRDHQLLTLNEKELIAQARELATKIDAFLTEREQSVLSKLIALGGSMEEESFEVQVKV
                     KVEEPNLIVQNVKRPEIEINAYKHYRQFDEYFLFEDPNQGRLRYREDSLINEKGDIVN
                     VRSRLTLLGVKREDEMGEVLLSRSRFLAPAAQSLRFYREYFKPKSEISVEKNRFRWHI
                     KYKNTEFFINLDEVKEPAMGHFLEVKSRTWSRKDADLKADLVRELLDILGVGNAETVT
                     QDYIEILTA"
     gene            1105..>1753
                     /locus_tag="PLR93_11790"
     CDS             1105..>1753
                     /locus_tag="PLR93_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27698.1"
                     /translation="MEPFRKPIATFFATSFVVTAVMAILFLNFDRRAFSAETYQQAFA
                     REDFYNKIPNLMAQSIVSGANMGQLPTVTQGMSLETWENFIRILLPPEVLKPIGDDVL
                     ISTFAYLNMERNSVQVNLTPVRTSMMSESGSQAILFLLNGLPACSAEQIAQITFDLLS
                     GEQVQLCNPPTDMLPILMPVIQGEMQATAAVIPDQLTLFTAPFQDDPREKLQTARL"
BASE COUNT          460 a          484 c          394 g          415 t
ORIGIN      
        1 acctcgacat gttcgaagaa gtgcgcctcg cctctttcgt cgccaaggct gcttcaaacg
       61 acccaaccgt tctccccgcc gccaccgcgt tgaccatggc aactcgcctg ggggctcagg
      121 cattgcacat cggtcacctt accggctcgt tggaagttgg caaacgagca gacctgattc
      181 tcgtggatac caatccgctg cacaacacac cgcgcttcaa gcgtgacccg cagaatgcct
      241 atgcccagtt ggtatacgct tctaaatcga cagacgttac cgatgtgatg gtcaacggca
      301 agtggctgat gcgcgatcat caattgctta cgttgaacga aaaagaactt attgctcaag
      361 ctcgcgaact cgcaacaaaa atcgacgcct tcttgaccga acgcgaacag tctgtgcttt
      421 cgaagctcat tgccctcggc ggttcgatgg aagaagaatc attcgaggtg caggttaagg
      481 tcaaggtcga agagcccaat ctcatcgtcc agaatgtgaa gcgccctgag atcgaaatca
      541 acgcctacaa gcattatcgt caattcgatg aatacttcct attcgaagat cccaatcaag
      601 ggcgtttacg ttaccgcgaa gatagcctta tcaatgaaaa aggtgacatc gtcaatgtgc
      661 gttcacgctt gactctatta ggtgtgaagc gcgaagatga aatgggagag gtgttgctct
      721 cgcgtagccg cttccttgca cctgccgcac agtcgctgcg tttttatcgt gaatacttca
      781 aacccaagtc tgagatttcc gttgagaaga accgcttccg ctggcacatc aagtataaga
      841 acaccgagtt ctttatcaac ctcgatgaag tgaaagaacc cgccatggga cacttccttg
      901 aagtgaaaag ccgcacctgg agccgaaaag acgcagacct caaagcagac ctcgtgcgag
      961 aactgcttga tattcttggc gttggcaatg ctgaaaccgt cacccaagat tacattgaaa
     1021 ttttgaccgc gtaatcaata ccaagggcga cctgcctgcc atggtgttta tggcatcggg
     1081 tcgcccttat gaatttaatt aactatggaa ccctttagaa aaccaatagc aacattcttt
     1141 gccacatcgt tcgttgtcac agccgtgatg gcgatcttat tcttgaactt tgaccgccgc
     1201 gctttcagcg ccgaaaccta tcaacaagcg tttgcacgcg aagactttta caacaaaatc
     1261 cccaacctca tggcgcaatc cattgtatcg ggtgcaaaca tggggcaatt gccaaccgtc
     1321 actcaaggca tgagccttga gacttgggag aatttcattc gcattctgct accgcccgaa
     1381 gtcttaaaac cgattggcga tgatgtgttg atctcaacct ttgcctatct caacatggaa
     1441 cgcaactcgg tgcaggtaaa cctcacacca gtccgcacca gcatgatgag tgaaagcggc
     1501 tctcaggcga ttttattttt actcaatggc ttgcctgctt gtagcgccga acaaattgca
     1561 caaatcacct ttgacttgct ttctggcgaa caggtccaac tctgcaaccc gcccacagat
     1621 atgctgccca tactgatgcc cgtcattcaa ggagaaatgc aagcgactgc tgccgtcatc
     1681 cccgatcaat tgactttatt taccgcgccc tttcaagatg acccacgaga aaaactgcaa
     1741 accgcccggc tct
//
LOCUS       DAOQFT010000540         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_48901_length_1751_cov_4.372642, whole genome shotgun sequence.
ACCESSION   DAOQFT010000540 DAOQFT010000000
VERSION     DAOQFT010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48901_length_1751_cov_4.372642"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PLR93_11795"
     CDS             <1..977
                     /locus_tag="PLR93_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNH27699.1"
                     /translation="IDKFMDDSFEVDVDALADGERVVVGAIMEHIEEAGVHSGDAACV
                     LPPYKVSAYHLEIMREYTEQLGLALGVRGLMNVQFALKDEIVCVLEVNPRASRTVPYA
                     SKATNLNLAYKAAQVMAGKTLPELNVLQEPRVDGFFVKEAVMPFKKLPGTSIILGPEM
                     RSTGEVMGHASRFGHAFAKSQTAAGTGLPQSGAVLITVNDFDKSAGLKFARDMHNLGF
                     KLYATPGTADICAKAGLPVEVVEKAQDGSTQIVDLIRTGKIDLVLNTPLGANAHTDGV
                     EIRRAAIAMNVPLLTTLTAAMAAVSAIRALSKKELKYRSLQSHFAEKI"
     gene            991..>1751
                     /locus_tag="PLR93_11800"
     CDS             991..>1751
                     /locus_tag="PLR93_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH27700.1"
                     /translation="MLNIVETENKHTSGVYAKQTLTIVRGQGASLFDVDGNEYLDCSS
                     GHGVANLGHAHPKVAEALYKQANTLITLFETFPNDQRAALMEKITALVPGMDRVFFCN
                     SGTEAVEASFKFSRISTGRKNIIAAMRAFHGRTYGSLSATFNKKYREGFEPLLPGVSH
                     VAYNNIEALDKAVNEETAAVILEVVQGEGGVYPASAEYIQAARRICDERGALLIVDEI
                     QTGFGRTGKMFAVQHFDVTPDLLTCAKSLAGGVPMG"
BASE COUNT          437 a          389 c          475 g          450 t
ORIGIN      
        1 tgatcgataa atttatggat gactcgttcg aagtggatgt cgatgcattg gcagatggtg
       61 aacgtgtggt ggtgggtgcc atcatggaac acattgaaga ggctggggtt cattctggcg
      121 atgcggcttg tgtgttgccg ccgtataaag tcagtgcata tcatcttgaa attatgcgtg
      181 aatataccga gcaacttggg cttgcccttg gcgtgcgcgg actgatgaac gtacaatttg
      241 cactcaaaga tgagattgtt tgcgtgttag aagtcaaccc gcgcgcttca cggacagtcc
      301 catatgccag caaagccacg aatttgaatc ttgcttataa agccgcgcaa gtgatggcgg
      361 gcaagacctt gcctgagttg aatgttctac aagaaccgcg agtggatggt ttctttgtga
      421 aagaagctgt gatgccgttt aagaagttgc ccggtaccag tatcatactc ggaccggaaa
      481 tgcgctcaac cggcgaagtg atggggcatg cctcgcgttt tggtcatgcc tttgctaaat
      541 cacaaactgc tgccgggacg ggacttccac agagtggggc ggtcttgatc acggtgaatg
      601 actttgataa gagtgctggt ttgaagtttg cacgagatat gcataattta ggtttcaaat
      661 tgtatgccac gcccggtact gcggatattt gtgcaaaggc tggcttacca gttgaagtgg
      721 ttgagaaagc gcaagatggg tcaacgcaga ttgtcgattt gatccgcaca gggaagattg
      781 accttgtgtt gaacactcca cttggtgcga atgcgcacac cgatggcgtg gagattcgtc
      841 gcgcagctat cgcgatgaat gtgcccttac ttactacgtt gaccgcagcc atggctgcgg
      901 tatctgccat tcgcgcactt agtaaaaaag aattgaaata tcgtagtctg caaagtcatt
      961 ttgcagagaa aatttaattg agaggtaatc atgttaaata tcgttgaaac tgaaaacaaa
     1021 cacacatcag gtgtatatgc caaacaaacc ctgaccatcg tccgcgggca aggagcatcg
     1081 ttgttcgacg tagatggcaa tgagtatttg gattgctcgt ctggtcatgg cgtagcgaac
     1141 ttggggcatg cgcatccaaa agttgcagaa gcgctttaca aacaggcgaa cacactcatt
     1201 actttgtttg aaacgtttcc caacgaccaa cgtgctgcat tgatggaaaa gattaccgct
     1261 ctcgttcccg gcatggacag agtcttcttc tgcaactccg gtaccgaagc cgtagaagcc
     1321 tcgttcaaat tctcgcgcat ttcaacggga cgaaaaaata tcatcgccgc catgcgtgct
     1381 tttcatggac gaacctacgg ttccctctct gccaccttca ataaaaaata tcgtgaaggc
     1441 tttgagccac tgttgcctgg cgtttcgcat gtggcataca acaacatcga agccctcgat
     1501 aaagctgtga atgaagaaac tgccgctgtg attctggaag tggtgcaagg ggagggtggt
     1561 gtgtaccctg catctgctga atatattcaa gcggctcgcc gtatttgtga cgaacgcggt
     1621 gctttgctga tcgtggatga gatccaaact ggttttgggc gcacaggaaa aatgttcgcg
     1681 gttcagcact tcgacgtgac gcctgatctg ttgacctgtg ccaagtccct cgctggaggc
     1741 gtgcccatgg g
//
LOCUS       DAOQFT010000541         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_49173_length_1745_cov_10.639053, whole genome shotgun
            sequence.
ACCESSION   DAOQFT010000541 DAOQFT010000000
VERSION     DAOQFT010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49173_length_1745_cov_10.639053"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PLR93_11805"
     CDS             <1..1094
                     /locus_tag="PLR93_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH27701.1"
                     /translation="FPRFQAFVDQKFLGIKLPYQHLPEIYSSRITTSTSLPSLLGLLE
                     TDVFPSLLIRQYAFLQVSEKGLIKLMDKGIPNLATFDIETLTQKAGEYIPSLSQQDDG
                     PRLILPLKVGTQTLGFWLLGKRDPDDLYPQVEIPILQSLANQTAIALSNILQTDQLRN
                     IYESNIDRVEKERKRVARDLHDSVLNQLANMRNSLDQRTLPAEFLSTYDGLKQRIREI
                     ISDLRPPILDQGLAYAISELVEDLREEASHMDINLDLQTSGERIPEKNETHLFFIVRE
                     ACMNVLKHANAKTLNISGNITPTRVELSIQDDGKGLNIDLQKEVNTLLEEEHFGLVGM
                     FERARLINADLNINSNENSGTTIHITWQS"
     gene            complement(1215..>1745)
                     /locus_tag="PLR93_11810"
     CDS             complement(1215..>1745)
                     /locus_tag="PLR93_11810"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNH27702.1"
                     /translation="GWSRSLYLRDFETEDHTQRVTEMTTRLARRLGLPEEEMNNLRRG
                     AQVHDIGKIAIPDQILLKTGKLTEEEWVVMRKHPLIAVEMLEPIPEFKPALVIPRSHH
                     EKWDGSGYPDGLVGSNIPLPARIFAFADVYDALTSDRPYRKAWSRSDALQFIRNETGR
                     HFDPALTSEFIRLMSE"
BASE COUNT          500 a          461 c          352 g          432 t
ORIGIN      
        1 tctttccgcg ctttcaagcc tttgtggatc aaaaatttct gggcattaaa ctgccctatc
       61 aacaccttcc tgaaatctat tccagccgta tcacaaccag cacctccctt ccgagcctgc
      121 ttggtttact agagacggat gtctttccaa gtctattgat ccgtcaatat gcctttttgc
      181 aggtcagcga aaaagggctg ataaagctta tggacaaagg catcccaaat ctcgccacgt
      241 ttgatattga aaccctgacc caaaaagcag gagaatacat tcccagcctt tctcagcaag
      301 atgacggacc tcgcctcatc cttccgctca aagttggtac ccaaaccctt ggcttttggc
      361 tactcggtaa gcgtgaccct gacgacttgt atccacaggt tgagattccc atcttacagt
      421 cgttggcaaa ccaaaccgcc atcgccctca gtaacatcct tcaaaccgat caacttcgga
      481 acatctacga atccaatatt gaccgagttg aaaaggagcg aaaacgtgtt gcacgcgacc
      541 tgcatgacag tgttcttaat caactagcca acatgcgcaa cagcctcgac caaagaaccc
      601 ttcccgctga atttctatcc acctatgatg gtctcaagca acgcatccgt gaaatcatca
      661 gcgaccttcg tccgcccata ctcgatcaag gactcgccta tgcaatcagc gagttagtag
      721 aagacctgcg agaagaagca agccacatgg acattaatct tgacctccaa accagtggcg
      781 aaagaatccc tgagaaaaac gaaacccacc ttttctttat tgtgcgtgaa gcctgtatga
      841 atgttttgaa acatgccaat gcaaagacat tgaatatctc aggaaatata acccccaccc
      901 gagtggagct atccattcaa gatgatggta aaggcttaaa cattgacttg caaaaagaag
      961 tcaacacatt actcgaagaa gaacacttcg ggcttgttgg catgttcgaa cgtgctcggt
     1021 tgattaatgc agatctgaac ataaactcca acgaaaattc tggcacaacc atacatatta
     1081 cctggcagtc ttaataaaac tgtcttttca cttttaaaac attacacgcc gggccttaca
     1141 ccggcgtgca ccccaggtcc cctaatcgca atcggatatc ccctttcctg ttccaactgc
     1201 gctggaaagc aaggttattc agacatcaaa cgaataaatt cagaagttag ggcagggtca
     1261 aagtgacgcc ccgtctcatt acggataaat tgaagagcat cgctgcgaga ccatgcttta
     1321 cgatatgggc ggtcagacgt aagtgcatcg taaacatcgg caaaggcgaa gattcgtgca
     1381 ggcaaaggaa tgttcgatcc cactaatcca tcggggtatc cagaaccgtc ccatttttcg
     1441 tgatgtgagc gtggaattac aagtgcaggt ttgaactcgg gaataggttc taacatctcc
     1501 acagcgatca atggatgttt acgcatcacc acccactcct cctcagtcaa cttgccggtc
     1561 ttcaataaaa tttggtctgg gatggcgatc ttcccaatat catgaacttg agcgccacgc
     1621 cggagattgt tcatttcttc ttcaggcaac cctaaccgac gagctagtct cgtggtcatt
     1681 tcagtgacac gttgtgtatg atcttctgtt tcaaaatctc gtaaatacaa ggaacgtgac
     1741 cagcc
//
LOCUS       DAOQFT010000542         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_49187_length_1745_cov_3.830769, whole genome shotgun sequence.
ACCESSION   DAOQFT010000542 DAOQFT010000000
VERSION     DAOQFT010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49187_length_1745_cov_3.830769"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLR93_11815"
     CDS             <1..730
                     /locus_tag="PLR93_11815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNH27703.1"
                     /translation="PPPAPGQRLHPLAAESCLLTWLTDGKQGLRQFVKRVFHWRVGFQ
                     WYLIVLFSIGFIAYLAQGLNMLLFGGHPIALQLPAGIPKEGWVVALPIVFVVNIFLGG
                     PLAEDIGWRGYALPKLRENMDVFSASLIIGSVWVLWHLPFFWIPEGRSAVGGIPVLWF
                     GLMTVAWSVLFAWVYVNTESILMPVLFHAAVNTTLGTLGVLGQSDGSLTSVMLNTLLT
                     WVVVGVIVLIAGKDLTFNKKASPV"
     gene            complement(749..1063)
                     /locus_tag="PLR93_11820"
     CDS             complement(749..1063)
                     /locus_tag="PLR93_11820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNH27704.1"
                     /translation="MYIFQVHHYIKPEGVEAYRAVTLENAQKTRLEPGVLRFEFFQDA
                     ADPTHFSLFEVYADMAARDTHLQTEHFLKWKDVYLATQARKGEGHEFTAIYPAEGDWK
                     KT"
     gene            complement(1135..1497)
                     /locus_tag="PLR93_11825"
     CDS             complement(1135..1497)
                     /locus_tag="PLR93_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27705.1"
                     /translation="MQSPEKPSRLGTISFILAILVVVVWCIYLIIFAATIDGGLNFGL
                     NDETAGYLVVLGGSAVMIILTILITLSGIITGALALRNKDSKRGLAIAGLVLNFLCFA
                     PYCLLLIFIAFSAIPTGS"
     gene            complement(1550..>1745)
                     /locus_tag="PLR93_11830"
     CDS             complement(1550..>1745)
                     /locus_tag="PLR93_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNH27706.1"
                     /translation="PDDVPWQRVINSQGKISERPGAERQRPLLEAEGVVFDAKGKVDL
                     KKFGWSGKDESDIPKQAAMF"
BASE COUNT          378 a          413 c          448 g          506 t
ORIGIN      
        1 gcccccacca gcgcccggcc agcgcctgca ccccttggcc gccgagtcct gcctgctgac
       61 gtggctcacc gatggcaagc agggtcttcg tcaatttgta aagcgtgtgt tccactggcg
      121 agttgggttt cagtggtatc tgatcgttct attcagcatt ggcttcattg cctatcttgc
      181 gcagggactc aatatgcttt tatttggtgg tcacccaata gcgcttcaac ttccggctgg
      241 cattccaaag gagggctggg tcgttgcact tcccattgtc ttcgttgtca atatattttt
      301 gggcggcccc ctggctgaag atatcggctg gcggggttat gccctgccaa agttacggga
      361 gaatatggat gtgttctctg cgagtctgat catcggctcg gtatgggttt tgtggcatct
      421 tccatttttt tggatacctg aaggcaggag tgcagtgggt ggtattccag ttttgtggtt
      481 tggcctgatg acggttgcct ggtcggtgtt atttgcctgg gtgtatgtaa ataccgagag
      541 cattctcatg cctgttcttt ttcatgccgc agtcaatacc acgcttggga cattgggtgt
      601 tctgggtcag tccgatggct cgctgacatc ggtcatgttg aatacacttt taacctgggt
      661 cgtcgttggt gtgatcgtcc tcattgcagg caaggatctg acattcaata aaaaagccag
      721 cccggtttag gctggcttgg ttggacgatc aggttttctt ccaatctcct tctgcgggat
      781 agattgcagt aaattcatgg ccttcgcctt tgcgcgcttg tgtggcgaga taaacatcct
      841 tccactttag aaagtgctcg gtttgcaaat gtgtatcccg cgccgccata tccgcataaa
      901 cttcgaagag actgaagtgc gttgggtctg ctgcatcttg aaagaattca aagcgcagta
      961 caccgggttc aaggcgggtc ttttgcgcgt tctccaaggt gacggctcta taggcttcta
     1021 cgccttctgg tttgatgtaa tgatggactt gaaagatata catggtctgc ctccgttgtt
     1081 caaactgtac ttgaaagcaa tccgggcaaa ctatgcaatt tcattgcaag agggttaact
     1141 tcctgtggga atggcgctaa atgctataaa aatcaacaaa agacaatatg gggcaaagca
     1201 tagaaaattt agaaccaagc ccgcaatggc aagcccgcgc ttggagtctt tgtttcgcaa
     1261 ggctaatgcg cccgtgataa tacctgaaag tgtgatcagg atcgtgagga ttatcatcac
     1321 cgcactgccg cccaagacca caaggtaccc tgcagtttca tcattcaagc caaagttgag
     1381 tccaccgtcg atggtggcgg caaagatgat gaggtagatg caccaaacta ctacaaccag
     1441 aatggcaaga ataaaagaga tagtgccaag ccgtgaaggt ttttctgggg attgcatgag
     1501 attctccttg ataaagtttt ttctctgatg cttttttcat tcgctgtttt caaaacatcg
     1561 ccgcttgttt tggaatatca ctttcgtctt tgccgctcca gccgaatttt ttcagatcta
     1621 ctttgccttt ggcgtcgaaa accacaccct ccgcctcaag cagtggacgt tgacgttctg
     1681 ctcctggacg ttcgctgatc ttgccttgtg agttgatgac tctttgccac ggcacatcat
     1741 cggga
//
LOCUS       DAOQFT010000543         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_49273_length_1743_cov_7.158175, whole genome shotgun sequence.
ACCESSION   DAOQFT010000543 DAOQFT010000000
VERSION     DAOQFT010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49273_length_1743_cov_7.158175"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLR93_11835"
     CDS             complement(<1..414)
                     /locus_tag="PLR93_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HNH27707.1"
                     /translation="MHHVHHDLKVYGGIKLFGGTGSPELSQKIAEYLDLPLSGREVIE
                     FPNENLFVKLNGSVRGQDVYVIQTTSSPVHRNLMELLIMIQTLKLDSAARITAVVPYL
                     CYGRSDKKDQPRVPITARLVADMLEAAGADRYMTLD"
     gene            complement(420..974)
                     /locus_tag="PLR93_11840"
     CDS             complement(420..974)
                     /locus_tag="PLR93_11840"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNH27708.1"
                     /translation="MSIVYGKRIRLRAVEREDVQKFHEWINDPEVTLNLAMYLPMSMQ
                     DEMNWFEGLSKRDPHEKPFAIEIRKGKAWKLIGNCGVFGIEFTNRNGELGIMIGDKSE
                     WNKGYGAEAMSLLQRHCFETLNLNRVYLRVYEENVPAVRAYEKAGFVLEGRQRQAVYK
                     KGKYEDVLFMSVLRSEWDTRMKEK"
     gene            complement(971..>1743)
                     /gene="meaB"
                     /locus_tag="PLR93_11845"
     CDS             complement(971..>1743)
                     /gene="meaB"
                     /locus_tag="PLR93_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNH27709.1"
                     /translation="AIDPSSPFTGGAVLGDRVRMRDLSGDPNVFIRSMASRGSLGGLA
                     QATANVVQVFDAAGFDVVIVETVGAGQSEVDIARLAHTTLVVEAPGLGDDIQAIKAGI
                     LEIADILVVNKGDRPGVENTEKALKTMLELAHPTERVFKHHGTSMRVSAPKTTANAKM
                     WIPPIQRTISTEGIGIAELAEAIARHVEHLHLSGDWATRERTRLEVELEALIREGLMT
                     KFREQLTAEAYEKVIESVVERNVSPWEAAKGLLKGYSK"
BASE COUNT          427 a          500 c          376 g          440 t
ORIGIN      
        1 gtcgagcgtc atgtagcgat ctgcaccagc ggcttcgagc atatctgcca ccaaacgggc
       61 agtgatcggc acacgtggct gatctttttt atcggaacgc ccataacaaa gataaggcac
      121 aaccgctgta atgcgcgctg ctgaatccag cttcagagtc tggatcatga tgagcaattc
      181 catcaagttg cgatgaacag gagaagacgt cgtctgaatg acgtacacat cttgcccacg
      241 cacactgcca ttcaatttga cgaacaagtt ctcattgggg aactcaatta cctcgcgtcc
      301 acttaatgga aggtcgaggt actctgcgat cttttgcgat aattcgggcg aacccgtccc
      361 accgaataac ttaatccccc cgtacacttt caaatcgtga tgaacatgat gcatgtggat
      421 tacttctcct tcattcgtgt gtcccattcc gagcgcaaca cgctcatgaa gagaacatcc
      481 tcgtattttc cttttttata aacagcttgg cgctgtcttc cttctaaaac aaaaccggct
      541 ttttcataag cacgaacggc aggcacattt tcttcgtata cccgtaaata gacacggttt
      601 aagttcaagg tctcaaaaca atgacgctgt aacaaactca tggcttctgc gccgtagcct
      661 ttattccact ctgatttatc gccgatcatg attccaagct cgccattgcg attcgtaaac
      721 tcaatcccaa acacaccgca gttcccgatc aacttccaag ccttgccttt gcgaatttca
      781 atggcaaaag gtttctcatg cgggtcacgt ttcgacaagc cctcgaacca attcatttca
      841 tcctgcatcg acatgggtag gtacattgcc aggttgagcg tcacttctgg gtcattgatc
      901 cactcatgaa acttttgcac atcttcacgt tccaccgcgc gcaaacgaat tcgttttcca
      961 tatacgatgc tcatttggaa tatcctttca acaagccttt tgccgcttcc caaggcgaaa
     1021 catttctttc gacaactgac tcgataacct tttcatacgc ctcagccgtt aactgctctc
     1081 gaaacttggt catcaatccc tcgcggatca acgcctctaa ctcgacctcg agccgggttc
     1141 gctcacgagt tgcccaatcg ccactcaagt ggaggtgctc cacgtgccga gcaattgcct
     1201 cagccaattc tgcaataccg atcccttcag tggagatcgt ccgttgaatg ggcggaatcc
     1261 acatcttggc gtttgcagtc gtcttcggcg cagagactcg catggacgtg ccatgatgct
     1321 tgaaaacacg ttcggttggg tgcgccaatt caagcatggt cttcaaagct ttctcggtat
     1381 tctctacacc gggtctatcg cctttattga ccacgagaat atctgcgatc tcaaggatac
     1441 cggctttaat ggcttggatg tcgtccccaa gcccgggagc ttcaacaact aacgtcgtgt
     1501 gggcgaggcg ggcaatatcc acttcacttt gacccgcacc caccgtttcc acaatgacca
     1561 cgtcaaaccc agccgcgtca aagacctgca cgacatttgc ggttgcctgt gctaatccac
     1621 cgagtgagcc gcgcgaagcc attgaacgga tgaagacatt cgggtcgcca gaaaggtcgc
     1681 gcatgcgaac tcgatctccc aacacagcgc cgcccgtgaa tggactggac ggatcaatgg
     1741 caa
//
LOCUS       DAOQFT010000544         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_49535_length_1738_cov_2.976827, whole genome shotgun sequence.
ACCESSION   DAOQFT010000544 DAOQFT010000000
VERSION     DAOQFT010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49535_length_1738_cov_2.976827"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLR93_11850"
     CDS             <1..544
                     /locus_tag="PLR93_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH27710.1"
                     /translation="ICLDRTTTGDGIISALQVSNHHIQGWFTAIPASDYFTRQGEVWS
                     AFDNVDHVIKAVKPIAKALKLPRLALQTMFGKPQHSSRTYEEVCTVYSDEIAKGAKAA
                     GPFLPSEAPVTDKEQAKAEQLQKVSKIIEDLVSALEKWEEDALDQCQLPHPLIGNLTM
                     REMLFFTIHHNLRHARLEGD"
     gene            complement(628..1389)
                     /locus_tag="PLR93_11855"
     CDS             complement(628..1389)
                     /locus_tag="PLR93_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27711.1"
                     /translation="MKTGAHPYAVVFSLFLTLSVTACGMTVPQATTTATSVPTSTNTA
                     LPTLTPTVTQTPTKTPVPTSTPNFAATQQYEGFSTWVGKLIDEGIIPSKDGDYHAVED
                     FSKAYAKQAYFTWKLYDNFSANNFIVQARARITNATTENVFKSGCGLIFADIFDAHAL
                     FFSLDGNANYRIDGADRGSKYIDSALFQNPDGVMLTMVLYNHALRFYVNDQMALSGIT
                     IYGGPFQSGPAILSGTSEGFGTQCDFTETVLWRIR"
BASE COUNT          500 a          412 c          419 g          407 t
ORIGIN      
        1 catctgcctg gatcgaacca cgaccggcga cgggattatt tccgccttgc aagtttctaa
       61 tcaccacata caaggctggt ttaccgccat tcccgcatca gattatttca cgcggcaggg
      121 cgaggtttgg tctgcgttcg ataatgtaga tcatgtcatc aaagcggtaa aaccgatcgc
      181 gaaagcattg aagttgccgc gattggccct gcaaacaatg tttggaaaac cacagcactc
      241 ctcgcggact tacgaagaag tctgcactgt ttactctgat gagatcgcga aaggcgcaaa
      301 ggcagccggg ccattccttc cgagtgaagc gccagttaca gataaagaac aagccaaagc
      361 agagcaatta caaaaggtct caaaaataat cgaagacctg gtctctgcat tggaaaaatg
      421 ggaggaagat gcgctcgatc aatgccaact ccctcaccca ctcattggga atttgaccat
      481 gcgggaaatg ctcttcttca ccattcatca caacctgcgc catgccagac tggaagggga
      541 ttaaggtggg ctggatactg aggtaggtaa aaccaaaaat ggcctccaca agatcatgga
      601 ggccattttt ttcattgtaa ccaactgtta tcggatacgc cataaaaccg tttctgtgaa
      661 atcgcattgg gtcccaaatc cttcagatgt tccggagaga atggcgggac ctgattggaa
      721 cgggccgccg taaatcgtga ttcctgaaag agccatctga tcgttcacat agaagcgaag
      781 agcatgatta tatagcacca tggtcaacat aaccccatca gggttctgaa aaagggccga
      841 gtcgatgtac ttcgaaccgc ggtcagcacc atcaatccga taatttgcat tcccatcaag
      901 agagaaaaac aaggcatggg catcaaagat atctgcgaaa atcaatccgc aaccggactt
      961 gaacacattc tccgttgttg cattggtaat tctggctctt gcctggacga taaagttatt
     1021 agcggagaaa ttatcgtaga gtttccaggt gaaataggct tgcttggcgt aagcctttga
     1081 aaagtcttca acggcatggt aatcaccgtc cttcgaagga atgatcccct catcaataag
     1141 ttttcccacc cacgtggaga atccttcata ttgctgggtg gccgcgaaat tcggcgttga
     1201 tgtgggaaca ggtgtttttg taggcgtctg agtgactgtg ggtgttaaag tgggcagggc
     1261 cgtatttgta gatgtgggaa ccgatgtggc agttgttgtc gcttgaggga cggtcatacc
     1321 acacgcagtg acagaaaggg ttagaaaaag tgaaaacact acagcatatg gatgggcacc
     1381 ggttttcata ttatctcctt gagttttgat gttctaaaat aaccatacac tctttattgg
     1441 aaaataactg ccaaaaaccg ccaaactttc aaagaagttc aaccatttgg cggataacct
     1501 ccgaaatccg gccgggttat aatacacaac ttccgcaaca aaaccgatcc attaggagag
     1561 agagatgcta aacaagctgt tattccatcg attccccagc ctgaaaagct ggctaacccg
     1621 catccaatac gagatgatct cggccatgga ccgggacaag aacgccatct tcatgaactt
     1681 tgggtacgcg caccacgagc aggaacccat ttccctggcg ccggatgagg agatccac
//
LOCUS       DAOQFT010000545         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_50729_length_1715_cov_7.448193, whole genome shotgun sequence.
ACCESSION   DAOQFT010000545 DAOQFT010000000
VERSION     DAOQFT010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50729_length_1715_cov_7.448193"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLR93_11860"
     CDS             <1..615
                     /locus_tag="PLR93_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNH27712.1"
                     /translation="PYVEGKEEPTIAYLPLASLYAERWLEMTEDAFKGLAELKTINTE
                     LMTQKAIEGMIRDADVVYVPGGNTFLLNHRLHASGVMPFLQKRLQSGLPYVGFSAGAI
                     LCGPNILTSKDLNAVSTSFFKGLNAFQFNVSPHYPLDGYGQSVQDDWLADYHFFHDNP
                     VIMLCDGSYIKSEKGKTSLVRGEAYILRKDSEKERLEEGQIITL"
     gene            625..1122
                     /locus_tag="PLR93_11865"
     CDS             625..1122
                     /locus_tag="PLR93_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNH27713.1"
                     /translation="MTEQWMPENKEELISAIEREWKLLISLAETLTDEQMTTLDEGGW
                     SPKDNLAHLTEWMNILMGYHIDKRPYYDVVGVSQEELPQWSVEAINPILFKRNQLRPR
                     AEVMDHLKRVYGLLVKKLDETPFEELLAPRHPDRPDPLILWVLGDTTEHFQEHRETIE
                     RGIKT"
     gene            1123..>1715
                     /gene="map"
                     /locus_tag="PLR93_11870"
     CDS             1123..>1715
                     /gene="map"
                     /locus_tag="PLR93_11870"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNH27714.1"
                     /translation="MIATDEKDINGLRAAGKVCAQAMQLMMESAKPGMTTLELDQIGE
                     DFLKKNGAKSAPRVMYNFPGGTCISVAPVIAHGIPGEHVIQDGELINIDVSAELNGYF
                     GDTGASMVVGTPNPEYQKMLDATKATLDKVLTLVRAGKPLNIIGKMVQQEANKNGYNP
                     IFDLTGHGIGRQLHDKPSAVYNFHKPDDRRTLDNGLVL"
BASE COUNT          489 a          400 c          418 g          408 t
ORIGIN      
        1 ccatatgtgg aaggcaaaga ggaaccgacc attgcctatt tgccgttggc ttctttatat
       61 gccgaacgtt ggttggaaat gaccgaagat gccttcaagg ggttggcaga actgaaaaca
      121 atcaacactg agttgatgac ccaaaaagca atagaaggca tgatccgcga tgcagacgtt
      181 gtgtatgtgc ctggtgggaa tacctttttg ctaaaccatc gtctacatgc tagtggggta
      241 atgcctttct tgcaaaagcg tcttcaatct ggtttgcctt atgttgggtt cagcgcgggc
      301 gccattctgt gtggaccaaa catcctaacc tcgaaagatt tgaacgcagt cagcacatcc
      361 tttttcaaag gcttgaatgc atttcagttc aatgtctcgc cgcattatcc gttggatgga
      421 tatggtcagt ccgtgcagga tgattggctg gcagattatc acttcttcca cgacaaccct
      481 gttatcatgc tgtgcgatgg atcatatatt aaaagcgaaa agggcaagac cagccttgtc
      541 cgaggggaag cgtacatttt gcgaaaagac tcagaaaaag aaagacttga agaaggtcaa
      601 ataatcacgt tataggagga agcgatgact gaacaatgga tgcctgaaaa taaagaagaa
      661 ctgatttctg ccattgaacg tgagtggaag ttgctgatta gccttgccga aacgctaacc
      721 gatgaacaaa tgaccacact cgacgaaggc ggctggtcac ccaaagacaa ccttgctcac
      781 ctcaccgaat ggatgaatat cttaatgggc tatcatatcg acaaacgccc atattacgat
      841 gtcgttggag tctcgcaaga agagttgcct cagtggagcg tagaagcgat taatcccatc
      901 ttgtttaagc gcaatcaact ccgcccgcga gcagaagtca tggatcacct aaaacgcgtc
      961 tatggacttc tggtgaaaaa attagacgaa actccctttg aagaattgct tgctccgcgc
     1021 cacccagacc gacccgaccc tttgatctta tgggtcttgg gcgatacgac ggaacatttt
     1081 caagaacatc gtgagactat tgaaagagga atcaaaacct aaatgatcgc aactgacgaa
     1141 aaagatatca acggcttaag agccgcaggc aaagtctgtg cccaagccat gcaattgatg
     1201 atggaaagcg ccaaaccggg catgaccacg ctcgaactag accagattgg cgaggatttt
     1261 ctcaagaaaa atggcgccaa gtctgcgccg cgtgtgatgt acaacttccc cggcggcact
     1321 tgcatcagtg ttgcgccggt cattgcacat ggtattccgg gtgagcatgt gattcaagat
     1381 ggcgaactga tcaacattga tgtctctgct gagctgaatg gctactttgg agatactggt
     1441 gcttctatgg tcgtgggcac gcccaaccct gaatatcaaa aaatgttgga tgccaccaaa
     1501 gccaccttgg ataaagtcct taccttggtt cgtgctggca aaccgctcaa catcattggc
     1561 aagatggttc aacaagaagc caacaagaac ggctataacc ccatctttga tttgaccggg
     1621 catggcattg gacgtcaact gcacgataag ccgtccgcgg tctataactt tcataagcca
     1681 gatgaccgcc gcacactgga taatggcttg gtcct
//
LOCUS       DAOQFT010000546         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_50786_length_1714_cov_6.242315, whole genome shotgun sequence.
ACCESSION   DAOQFT010000546 DAOQFT010000000
VERSION     DAOQFT010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50786_length_1714_cov_6.242315"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLR93_11875"
     CDS             complement(<1..334)
                     /locus_tag="PLR93_11875"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNH27715.1"
                     /translation="MPKRIGLFGGTFDPPHLGHLVLAAEAVSQFKLDTFLWVLTPDPP
                     HKLNTTITPLSHRLPMLQAMIAHDPVFELSRVEIDRPGPHYTIDTVRLLTQQYGDAEI
                     YLLIGGDSL"
     gene            442..1302
                     /locus_tag="PLR93_11880"
     CDS             442..1302
                     /locus_tag="PLR93_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH27716.1"
                     /translation="MHAVRAIEEGDYDPILLSEFMTETGDLGKLAQMLDSMANTILKR
                     NNQLTLLSKVIPIGVSLSAEKDFNRLLESLVEEARTFTNADAGSLYLVEDDKLRFVIS
                     RNTSLKMHMGGTSGTPITFVPVRMYNEDGSENRANIVSYAALTRQRINIADAYEAEGF
                     DFSGTKAFDENTRYRSKSFLTIPLETKEGKVMGVLQLINATDPKTKEIVPFQDDTVLE
                     ALILLATAALEGYIREAALRSEIAKLRIEIDQSRRAKQVEEITDTAFFRELKTKATEM
                     RSKTKKEDEE"
     gene            1443..>1714
                     /locus_tag="PLR93_11885"
     CDS             1443..>1714
                     /locus_tag="PLR93_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27717.1"
                     /translation="MTLFLQKHRHIFTLAGAILLALAVSACGENIDLSGAAGLGNSTD
                     VAATDVVATEETAVVAEAPTATPLPAATGHIIFASNRDGQTDLYMTT"
BASE COUNT          486 a          419 c          412 g          397 t
ORIGIN      
        1 gcagggaatc gccgccaatc agcaaataaa tttcagcatc tccatattgt tgagtcagca
       61 agcggactgt atcaatggtg taatgaggtc ctggtctatc gatctcaaca cgggaaagtt
      121 caaacacagg gtcgtgagcg atcatcgcct gcaacattgg aaggcgatga gacagaggcg
      181 tgatggtcgt attcaattta tgcggcgggt ctggggtaag cacccacaga aatgtatcta
      241 acttaaattg agagaccgct tctgctgcaa ggacaagatg accaagatga gggggatcga
      301 aagtaccgcc aaagagacca atgcgtttag gcatggcggt agtatatcgc atcctcatgc
      361 acagaatctt ggggtatact gaaaaacatg tcacagtcgc agcggacagt tattagttca
      421 acatctcccc ttaagaagat catgcatgcc gttcgcgcca ttgaagaggg tgattatgac
      481 ccaattctct tgagcgaatt catgactgag actggggatc tggggaaact ggcgcaaatg
      541 ctggattcga tggcgaacac cattttgaag cgcaacaatc aattgacctt gttaagcaaa
      601 gtcattccga tcggggtttc actttctgct gaaaaagatt tcaaccgttt gctggagtct
      661 ttagtagaag aagcccgaac cttcacaaat gccgatgccg gttctttata tcttgttgaa
      721 gatgacaaac tgcgcttcgt tatctcgcgc aatacttctt taaaaatgca catgggtggg
      781 acgagcggca cgccgattac ctttgtgccg gtacgcatgt acaacgaaga cggcagtgag
      841 aaccgcgcca acattgtttc gtatgcggca ttgacccgcc agcgcatcaa cattgccgat
      901 gcctacgaag ccgaaggttt cgacttctcc ggtacaaaag cctttgacga aaacacccgt
      961 tatcgttcca aatcattcct gaccattccg ctcgaaacca aagaaggcaa ggtgatgggc
     1021 gtgttgcaat tgatcaacgc cactgaccca aagaccaaag agatcgttcc tttccaagac
     1081 gacaccgttt tggaagcgct gattttgctt gccaccgccg ccctagaagg ctatatccgt
     1141 gaggcggctc tcagaagcga aattgccaaa ctgcgcattg aaatcgacca gtcacgccgc
     1201 gccaaacaag tggaagagat caccgacacg gctttcttcc gcgaactgaa gaccaaagcc
     1261 actgaaatgc gctcaaagac gaaaaaggaa gacgaagaat aaggggtttg caaaattgtc
     1321 aacttttaag gttgttctgg ggacactttt gggggataaa tgaacactaa caatacggaa
     1381 atcaaaaaga tagtaaaaaa gtcctaatcc gcctgaaagt agccctattg ggtagaagat
     1441 ccatgaccct tttccttcaa aaacatcgac acatttttac cctagctggc gctattttgc
     1501 tcgcactcgc cgtaagcgcc tgtggcgaaa acattgatct gtctggtgca gcaggtcttg
     1561 gtaactctac agatgtagcg gctactgatg tagtcgccac cgaagaaact gccgtcgttg
     1621 cagaagcgcc cacagctact ccgctccccg ccgccaccgg tcacattatc tttgcttcca
     1681 atcgggatgg tcaaaccgat ttatacatga ccac
//
LOCUS       DAOQFT010000547         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_50981_length_1710_cov_5.693656, whole genome shotgun sequence.
ACCESSION   DAOQFT010000547 DAOQFT010000000
VERSION     DAOQFT010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50981_length_1710_cov_5.693656"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLR93_11890"
     CDS             <1..516
                     /locus_tag="PLR93_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27718.1"
                     /translation="IARAAELLHSPDIVQFGVVTETPEEWKAFIGKESNAGLYLAGDD
                     TTKELLQALVTWAHQNRGDGQFVLLLIDNLDAITHLDEQTQQNLRWLLLRGPSRRIWP
                     FITLNTKNAMEHKEWLEFFRTRLFGFTENPEEAYLLTGHSTLDHLQAGTEFAMRESGK
                     LLRFWLPSIYK"
     gene            532..768
                     /locus_tag="PLR93_11895"
     CDS             532..768
                     /locus_tag="PLR93_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27719.1"
                     /translation="MHTGLLWFDNDPRTTLSVKIQKAMDYYRKKFGRIPDLCLVHPSM
                     IGENQNQLELGKLVIRAYRPVMPGHFWIGTEDQK"
     gene            881..1309
                     /locus_tag="PLR93_11900"
     CDS             881..1309
                     /locus_tag="PLR93_11900"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH27720.1"
                     /translation="MSDIEEQIRQIFEGYKTAVFEKNVEAFLALYDENIHLFDMWEKW
                     SYNGKDAWREMVSNWFGSLGDEKVVVDFDSLTVTATNEIAVVHTFVTYKAVASNGNEL
                     RSMNNRLTAALKKSNGTWKIFHEHSSAPLDGNTLKATLQR"
     gene            complement(1353..>1710)
                     /gene="acpS"
                     /locus_tag="PLR93_11905"
     CDS             complement(1353..>1710)
                     /gene="acpS"
                     /locus_tag="PLR93_11905"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNH27721.1"
                     /translation="LRTGVDLIEISRVQEAIERHGLRFLNRIFTETEQHECGGRVSSL
                     AARFAAKEAVSKALGCGIGDVGWLEIEIRSDEKKAPHLVLYGEAERLAKMLGLTTWSV
                     SLSHTESHAIAFVVMM"
BASE COUNT          491 a          409 c          375 g          435 t
ORIGIN      
        1 atcgcccgcg ctgcggaact tcttcattca cccgatattg tgcaatttgg cgtcgtaact
       61 gaaactcctg aagaatggaa agcattcatt ggaaaagaaa gcaatgccgg gctatatctc
      121 gcaggtgatg acactacaaa agaacttcta caggcgcttg ttacctgggc acaccaaaat
      181 cgaggtgacg gccaattcgt tcttttatta attgacaatc tggatgccat cacccatctc
      241 gacgaacaga cccaacaaaa tttacgctgg ctcctgttgc gcggacccag ccgccgcatt
      301 tggccattca taacacttaa tactaaaaat gccatggaac ataaggaatg gctggaattc
      361 ttccgtacaa ggttattcgg ttttactgaa aaccctgaag aggcctattt attgaccgga
      421 catagcaccc tcgaccattt acaggctggc actgagtttg ccatgcgcga aagcggaaag
      481 cttttgagat tctggcttcc aagtatttat aaataaagaa agcaggtttc catgcatacc
      541 ggattattat ggtttgataa tgacccacgt accacattga gcgtaaagat ccaaaaagcc
      601 atggactatt accgtaaaaa attcgggcgc attccagatc tatgccttgt acatccaagc
      661 atgatcgggg agaatcaaaa tcaacttgag ttgggcaagc ttgtcatccg cgcatatcgc
      721 cccgtaatgc ctggccactt ttggatcggt acagaggatc aaaagtaatc ctgtccgtca
      781 agtcgagata tagtatttcc tttcgccatt tcctatatct ccttgtgccc tcccatataa
      841 ctggcatatg ggagggcttt ttttataaaa ggagagaatc atgagtgaca ttgaagaaca
      901 gatcagacag atatttgaag gatacaagac ggctgtgttc gaaaaaaatg tggaagcatt
      961 tctcgcttta tacgacgaaa acatccacct cttcgatatg tgggagaaat ggtcatataa
     1021 cggcaaagat gcctggcgtg agatggtatc caattggttt ggttccctgg gagacgaaaa
     1081 ggtagtggtc gatttcgaca gtttaacagt aacagccacc aatgagatcg cagtcgttca
     1141 tacctttgtt acctacaaag cagtggcttc caatgggaat gaactacgtt ccatgaataa
     1201 tcgattaaca gcagccctca aaaaatcgaa cggaacgtgg aaaatctttc atgaacattc
     1261 ttcagcgcca cttgatggaa atacgttgaa ggcaaccctt caacgttagt agcaacaaaa
     1321 aagccttctt cacagagaag gcttttttgt tgctacatca tgaccacaaa tgcaattgca
     1381 tggctttctg tgtggcttag gctgacagac caagtcgtca gccccaacat cttcgccagt
     1441 ctttcggctt ctccatataa aaccaaatgg ggagccttct tttcatctga acgaatttct
     1501 atctccagcc agcctacatc accgataccg cagcccaagg ctttggatac ggcctccttc
     1561 gctgcaaatc gagcggccag ggatgagact cgaccaccgc actcgtgctg ctcagtttca
     1621 gtaaagatgc gatttaagaa gcgcaagccg tggcgttcta tcgcctcctg aacgcggctg
     1681 atttcgatca gatccacgcc ggtacgaagg
//
LOCUS       DAOQFT010000548         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_51129_length_1707_cov_3.678571, whole genome shotgun sequence.
ACCESSION   DAOQFT010000548 DAOQFT010000000
VERSION     DAOQFT010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51129_length_1707_cov_3.678571"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLR93_11910"
     CDS             <1..225
                     /locus_tag="PLR93_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH27722.1"
                     /translation="RVDGLEILRIIKEHEEIKSIPVVILTTSEADKDVSHAYEHYVNS
                     YLVKPVGFEEFSQLMNDLGVYWLGWNKQPK"
     gene            251..1618
                     /locus_tag="PLR93_11915"
     CDS             251..1618
                     /locus_tag="PLR93_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH27723.1"
                     /translation="MIRILLVEDEDPHAELIQRAFEDQNPEIYLLRVKSLTEARAARS
                     QQEPTLIIADWRLPDGESMELLPNHHDPLATPIILMTSYGNERIAVEALKSGALDYVV
                     KSPESMLDMPHLAQRALEQWDARAERVRMQKALLESEAQFRLLAENASDMIARLSTDG
                     HMLYVSPASKSILGYTPEEMVGAQIFDLIFEEDKSRIKDIFRRAPVDTYTVAYRALHK
                     EGHYLWLESSARAILNKQTGEITEIQSASRDITERKRAEEALQVAHNQLREAYEKTIE
                     GWVRALDLRDRETEGHTQRVTELTLKVAANLGFTQEELAHIKRGALLHDMGKMAIPDE
                     ILQKPGPLNESEWEKMRQHPVYAYDMLSPIAYLHPALEIPYYHHERWNGSGYPHGLKG
                     EQIPLSARLFAIIDVWDALRSDRPYRKQLPREQVIAYLQEKSGSLFDPKLVAVFLSVI
                     ETVEN"
BASE COUNT          486 a          444 c          403 g          374 t
ORIGIN      
        1 agggtagatg gtttggagat tttacgaatt atcaaggaac atgaagagat caaaagtatc
       61 cccgtggtga ttctgactac ttcagaagcc gataaagatg tcagccacgc ctatgagcat
      121 tatgtcaata gctatcttgt taaaccagtt ggtttcgagg aatttagtca acttatgaat
      181 gatcttgggg tttattggtt gggctggaac aaacagccca aataaaaaat tttgcaccat
      241 gccgaatgaa atgatccgca ttttacttgt agaagatgaa gacccgcacg ctgaactcat
      301 tcagcgtgca ttcgaagacc aaaatccaga aatttacctt ctacgcgtta agtctctaac
      361 tgaagcccgc gccgcccgat ctcaacagga gccaactctg atcattgcag attggcgcct
      421 gccagatggt gaaagcatgg agttacttcc aaatcaccac gatcccctcg caactccaat
      481 cattcttatg acaagttacg gcaatgaacg cattgctgtg gaggcgttga aatcaggcgc
      541 gctggattat gtagtcaaat cgccagaatc catgctggat atgccccacc tagcccaaag
      601 agccttggaa caatgggacg cccgggcaga gcgagtgcga atgcaaaagg cactccttga
      661 aagtgaagcg caattccgcc tactggctga aaatgccagt gacatgattg cccgcttgtc
      721 cacagatggg catatgttgt atgtctcccc ggccagtaaa agcatcctgg gatacacccc
      781 cgaagaaatg gtgggagccc aaatttttga cttgatcttt gaagaggaca aatcaagaat
      841 caaggatatt ttcagaaggg caccagtaga cacctacacg gttgcttatc gagcgttaca
      901 caaagaaggg cattatcttt ggcttgaatc ttctgcccga gccatcttaa acaagcaaac
      961 cggggaaatc accgaaattc aatcggcgtc tcgtgatatc acagaacgca agcgggcaga
     1021 agaggcctta caagttgcac acaatcaact ccgcgaagcg tatgaaaaaa ccatagaagg
     1081 ttgggtacgt gcgcttgacc tgcgtgaccg cgaaacagaa ggacacacgc aacgtgttac
     1141 agagttaacc ctcaaggtgg ccgcaaacct ggggttcacc caggaggaat tagcccacat
     1201 caaacgcggc gcgctcctgc atgatatggg caagatggcc attccggacg aaattcttca
     1261 aaagccgggg cccctcaacg aatccgaatg ggaaaaaatg cgccaacacc ccgtttatgc
     1321 atacgatatg ctttcgccaa tcgcctacct tcaccctgca cttgaaattc cttattatca
     1381 ccacgaacgg tggaacggga gtggatatcc acacggcctc aaaggcgaac agattccgct
     1441 gtcggcacgc ctatttgcca tcattgacgt ttgggatgcg ttaagaagcg accgccccta
     1501 ccgcaagcaa ctgcctcgcg aacaggtcat tgcctacctt caggaaaaat ccgggtcact
     1561 cttcgacccc aaactggttg cagttttcct atcggttatc gaaacagtcg agaactaatt
     1621 tgacacgtcc ccacacttcg agtatccttg cacccgtccg ggcaggcccg gcgcggcgaa
     1681 gcctttcgaa ccccgccagg atcgaaa
//
LOCUS       DAOQFT010000549         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_51172_length_1706_cov_5.449425, whole genome shotgun sequence.
ACCESSION   DAOQFT010000549 DAOQFT010000000
VERSION     DAOQFT010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51172_length_1706_cov_5.449425"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1044
                     /locus_tag="PLR93_11920"
     CDS             151..1044
                     /locus_tag="PLR93_11920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH27724.1"
                     /translation="MNILVLGTDSREVSAIQQIASGDGNTVIPVSSSAEAWQVIQKGG
                     IQVLVSDIDATDFVPFQLISKVRTLKDSTYTLLITSRNDDGLLPKGDDILHRPYTSAD
                     LKNRIEIAKRFIALTAKLVIVSQQLENQAAFDPLTGFLNRAGFLRQATGELERSRRSS
                     SPLCLIALDIDNFQVINDRFGSKVGDDVLEAVAKSIRDKSRPYDCIGRWTGDEFVIVI
                     PNIIGADAEKVAERIIAGVRGTRIEVADEPPLNVKISAGIAAIMRITSTTEVEPLIQN
                     ARQALTRAKEAGGNQVYLSFL"
     gene            complement(1039..1359)
                     /locus_tag="PLR93_11925"
     CDS             complement(1039..1359)
                     /locus_tag="PLR93_11925"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNH27725.1"
                     /translation="MNNIWLVMIVGGLITFGMRFSLIYLFGKFEIPESLRRALHYVPP
                     AVLSAIIFPEIFLSNGTLDISLSNARLPAGILAIIAAWYSRSTLVTILIGMAALFFFQ
                     WIKL"
     gene            complement(1356..>1706)
                     /locus_tag="PLR93_11930"
     CDS             complement(1356..>1706)
                     /locus_tag="PLR93_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27726.1"
                     /translation="LHYEKEGLAKYKYWFLFGAGISLWTNWQISTAIGVFLGAAIPEN
                     LSLDFALPVTFIAMIVPVMKKSPVIASALSAGVTALLAYHLPYKLGLILAALVGITVG
                     MLLENLQAAPKESK"
BASE COUNT          475 a          373 c          443 g          415 t
ORIGIN      
        1 agggcttatg agtcccctgc tctgccactg agctacctcg gcgtggtcaa agcggacgca
       61 atattactat gggggatgca aattgtcaac gtggatggaa tatcgtcaaa gaagcgaggt
      121 cacctgctat aatggggcaa aggtgcatcc atgaacatcc ttgtgttggg aacggattct
      181 agggaagttt cagccattca gcaaattgcg agcggagatg gaaatacggt tattccagtc
      241 tcttccagtg ctgaggcatg gcaggttatt caaaagggtg gcattcaggt tctggtgtcc
      301 gatatagatg ccacagattt tgtgcctttc cagctaattt caaaagttcg cactctaaaa
      361 gactcgactt acactctgtt gattacatct agaaatgacg atggattgct ccccaaaggc
      421 gacgatattc ttcaccgccc ttacacctca gcggatttaa agaaccgcat cgaaattgcc
      481 aagcggttta ttgcgttgac agccaaactt gtcatcgtca gtcaacagct agagaaccag
      541 gcggcgtttg acccgctaac tggcttcttg aaccgagctg gttttttacg tcaggcaaca
      601 ggcgagttgg aacggtctcg gcgttcgtct tcccccttat gcttaattgc attggatatt
      661 gataactttc aggtaatcaa tgatcggttt ggttcgaagg ttggcgatga tgttttagaa
      721 gcagtagcca agtcgattcg cgataagagc cgaccatatg attgcattgg acgttggacc
      781 ggtgatgaat ttgtgattgt gattccaaac atcattggcg cggatgcgga aaaggtggca
      841 gagagaatta ttgctggggt tcggggcacc cgcatcgagg tcgcggatga gccgcctttg
      901 aatgtcaaaa ttagtgcggg tattgctgcc atcatgcgaa tcacatcaac aaccgaagtg
      961 gagccgctca tacaaaacgc gcgccaagct ctgacgcgtg caaaagaggc ggggggcaat
     1021 caggtgtatt tgtcttttct atagcttgat ccattgaaaa aagaatagtg ctgccatgcc
     1081 aatcaaaatg gtgacgagtg tactgcggga gtaccatgcg gcgatgattg ccaagatgcc
     1141 agccggtaac ctggcgttgg ataaagaaat atccaatgtg ccgttgctca aaaagatttc
     1201 ggggaagatg atggcagaaa gcacagcagg tgggacgtaa tgtaatgccc ggcgcaagct
     1261 ttcaggaatt tcaaactttc caaacaaata aatgagggaa aaacgcatac cgaaagtgat
     1321 caaaccaccg actatcatga ccagccaaat gttattcatt ttgactcctt tggggcagct
     1381 tgaagatttt caagcaacat tccaaccgta atgcccacca aggcggcaag gatcaaaccg
     1441 agtttataag gcaaatgata agcgagtagg gcggtcacac cggcgctgag cgctgatgca
     1501 atgaccgggg attttttcat cacgggcacg atcattgcga tgaatgtgac gggcagagca
     1561 aaatcgagcg ataagttctc agggatggca gcaccaagga acacaccaat ggctgtgcta
     1621 atctgccagt ttgtccaaag agaaatacca gcgccaaata agaaccagta tttgtattta
     1681 gccagccctt ctttttcata atgcaa
//
LOCUS       DAOQFT010000550         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_51783_length_1694_cov_6.639414, whole genome shotgun sequence.
ACCESSION   DAOQFT010000550 DAOQFT010000000
VERSION     DAOQFT010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51783_length_1694_cov_6.639414"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PLR93_11935"
     CDS             <1..1145
                     /locus_tag="PLR93_11935"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH27727.1"
                     /translation="SNIGTWMQVIAQAWVVYQLGHSELTLGLVAFASAIPNLIVSPWG
                     GVIVDRISRRKVLIMTQSGAMVLSFILAWLTFTNIVQEWHVILLAALTGVITAFDAPA
                     RQAITPELVGKKDVANAIAMNSMMFNSARVVGPAIGGMMLATVGAAWCFTVNGISFLA
                     VLIGLWLMKLPPHRKSAQDVSPWKQLVGGLQYAASNIEISTLILLSLVFSIFGVSYTT
                     VLPAFVEKNLQLGATAFGWVNAVTGLGAVTGAFLLVHRISNNRRGLFLIVTNIAFPVT
                     LIAFAVTAFYPLSLVLAYLLGLGFMVQFTTINTLLQARVEDDFRGRVMGLYTLSFFGF
                     SPFGNLLMGALGEKFGLTFVMILFGILSLVLSRLVLARNPGIKALP"
     gene            complement(1229..>1694)
                     /locus_tag="PLR93_11940"
     CDS             complement(1229..>1694)
                     /locus_tag="PLR93_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNH27728.1"
                     /translation="TGDNAKVAAKIAARAGVDEFHADLLPQDKVTVLKSLQKKYGSVA
                     MVGDGVNDAPSLATADIGIAMGGAGTDVAIETADVVLMSDDLRKIPFSIGLARQARKV
                     VWQNLTFALAVIVLLIASAFGANLPLPLGVVGHEGSTVIVVLNGLRLLGYRA"
BASE COUNT          371 a          445 c          425 g          453 t
ORIGIN      
        1 tctccaacat cggcacgtgg atgcaggtca ttgcacaggc ttgggtggtg tatcagctcg
       61 gtcactccga gttgaccttg gggttggtcg cctttgcttc tgccattcca aacttaatcg
      121 tctctccatg gggcggtgtg atcgtagacc gcatatcgcg ccgtaaagta ttgataatga
      181 cccagtctgg tgcaatggtg ttgtctttca tcctcgcctg gttgaccttt accaacatcg
      241 tgcaggaatg gcatgtgatt ctactggcag ccttgacggg tgtgattacc gcctttgatg
      301 ccccggcacg tcaagccatc acaccggaac tggttggaaa gaaagacgtc gccaacgcca
      361 ttgcaatgaa ctcgatgatg ttcaacagtg cccgtgtggt gggacccgcc atcggtggca
      421 tgatgctggc cacggtcggt gcggcgtggt gctttacggt gaatggcatt tccttcctcg
      481 cggtgttgat tggtctgtgg ctgatgaagc tacctcccca tcgcaagtcg gcgcaggatg
      541 tttctccatg gaaacaattg gtaggtggtc tgcaatatgc ggcaagtaac atcgaaatct
      601 cgacattgat tctgctctct ctcgtattca gcatcttcgg cgtttcgtat acaaccgtct
      661 tgccagcctt cgtagaaaag aacttacagc ttggtgccac cgcctttggc tgggtcaatg
      721 ctgtgaccgg gctcggagcc gtaacaggcg cttttctatt ggtacatcgc atcagcaaca
      781 accggcgcgg actctttctc atcgtcacca atatcgcctt cccagtaaca ttaatcgcgt
      841 tcgcagtgac cgctttttat ccgctttcac tggtgctcgc ttatttatta gggcttgggt
      901 tcatggtgca attcacaacc atcaacacgc tgttgcaagc acgggtggaa gatgacttcc
      961 gcgggcgtgt gatggggctt tacaccctaa gctttttcgg cttttcaccc tttggaaatc
     1021 tcttgatggg tgcactgggc gaaaaattcg gtttgacctt cgtaatgatc ctttttggga
     1081 ttctttcgct ggtactctcg cgtctggtgc ttgcccgaaa cccagggatt aaggctttgc
     1141 cgtagaaagc tcatataaca aaacaaaaag cggatgaatg tattttgaca ttcatccgct
     1201 ttttgtttat tttcttcaat caaaaaattt acgcccgata ccctaacaat ctcaaaccat
     1261 tcaacactac tatcacggta ctgccttcat gccccaccac cccgagcgga aggggtagat
     1321 ttgcgccgaa ggcactggca attagaagca cgatcacagc caaggcaaag gtcaggttct
     1381 gccaaacaac tttgcgtgct tggcgtgcca acccaataga gaagggaatc ttgcgcaaat
     1441 catcagacat taagaccacg tctgcggttt cgatggcaac atcggttcca gcgccgccca
     1501 tggcgatgcc aatatcggca gttgcaagag atggagcgtc gttcacgcca tcgcctacca
     1561 tagcgacaga accgtatttc ttttgcagag atttcaagac tgtcacttta tcttgcggta
     1621 agagatcggc atggaattcg tccacaccgg cgcgggcggc gatcttggca gccactttgg
     1681 cattgtcccc agtc
//
LOCUS       DAOQFT010000551         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_52338_length_1684_cov_6.240025, whole genome shotgun sequence.
ACCESSION   DAOQFT010000551 DAOQFT010000000
VERSION     DAOQFT010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52338_length_1684_cov_6.240025"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..985)
                     /locus_tag="PLR93_11945"
     CDS             complement(176..985)
                     /locus_tag="PLR93_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH27729.1"
                     /translation="MTKLVKDLMHKGVITCKPDATLGQVATLLDQHQVHALFVTDRDG
                     RIVGVISDFDLMTGEWLSSDPESLNTMRTLTASDLMTKPVDTIEAATPLTEAADLFMQ
                     KKVGRFLVLEHEKPVGTISLSDFVASLSERIKSKREKVGDVMSHAILVCRGKTPVLSA
                     AYAMTSAGWRSVLVVDLNGKVIGVVSGHDLLHLVKNGVDEKLTVRDVMHPALTIDINA
                     SLREAADMLIQNHYHRLVVVDKENPDSFPLGAISTFDIVAEMARPDSVWQK"
     gene            1113..>1684
                     /locus_tag="PLR93_11950"
     CDS             1113..>1684
                     /locus_tag="PLR93_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNH27730.1"
                     /translation="MGKPLIPAQRREKIQEYLGIHQIARTADLMDLLEASEATVRRDL
                     EWLEQKGILERTHGGAVLNQRVIFEQEYQLRLKNYPDEKKQIGEFAATLIEEGDIVFI
                     NSGTTATQVLQHVPRNPRITVFTNNVSALVDIGDPGFSLNLTGGEFQARSNSLSGRFA
                     LDNLGLVFANKAILGVDGISLKHGLTVPTNP"
BASE COUNT          431 a          404 c          415 g          434 t
ORIGIN      
        1 ggtgataggc gatactgctt ccacccacgc cgccgccgat gatgacgacc tgggcttgtg
       61 ttgggaattc agacatgaaa gtctcctaag agcgtttacc acgaaggaca ctaaggtcac
      121 gaagtttttt ttatttttcc ctttgtgtac ttcgtgccct ttgtggttaa gatttttact
      181 tttgccaaac ggaatcgggt cttgccattt ctgctacaat atcgaacgtt gagatcgcac
      241 caagtggaaa cgagtctggg ttttctttat cgaccacgac tagtcggtga taatgatttt
      301 gaatgagcat atctgcggct tcgcgcaggc tggcgttgat gtcgatggtg agagcggggt
      361 gcatcacatc gcggacagtg agtttttcat ctacgccgtt cttgacgaga tgcaagaggt
      421 catgtccgct cactacaccg atgactttgc cgttcaagtc cacaaccagc accgagcgcc
      481 agccagcaga tgtcatggca taggcggcag aaagaactgg tgtcttgcca cggcaaacaa
      541 gaatggcatg cgacatcaca tcgccgactt tctcgcgttt agatttgatc cgctcggaaa
      601 ggctggcaac aaaatctgaa agcgagatcg tgccaactgg tttttcatgc tctaaaacta
      661 aaaaccgccc aacctttttt tgcatgaaaa ggtcggcggc ttccgtcaga ggagtagcgg
      721 cttcgatggt gtcaacaggc ttggtcatca ggtctgaggc ggtgagtgtc cgcatggtgt
      781 tgaggctttc agggtctgaa gaaagccatt cgcctgtcat caaatcaaag tctgaaatga
      841 ctcccacaat ccgaccatcg cgatctgtga cgaacaaggc atgcacttga tgttgatcga
      901 gcaaggtagc tacttgaccc agggttgcat cgggtttaca agtgatcacg cctttgtgca
      961 taaggtcttt gacaagtttt gtcatgaagt ctccaaaaaa gaagacccga caggttttgt
     1021 gagattaagc tgtcaggtct gtgttacaaa tccattataa gcaaaaaatc aaaaacttgc
     1081 aaattccttc agattcttat ataatccgtc agatgggtaa acccctcatt ccagcccaac
     1141 gtagagaaaa gattcaagag tatcttggca tccatcaaat cgcgcgtact gccgacctga
     1201 tggatttgct tgaagcttct gaagcaaccg ttcgccgcga cttggaatgg ttggaacaaa
     1261 aaggaatcct agagcgcacg catgggggcg cggtcttaaa tcaacgtgtg atcttcgaac
     1321 aggagtatca actccgtctc aagaattatc ccgacgaaaa gaaacaaatc ggtgagtttg
     1381 ccgccaccct tattgaagag ggtgatattg tttttatcaa cagcggtacc actgctacac
     1441 aagtattgca gcacgttccg aggaatccac gcattacggt ttttacgaat aacgtcagtg
     1501 cattggtgga tatcggggac ccgggctttt ctctcaatct gaccggaggc gaattccagg
     1561 ctcgttccaa ctctctttcc ggtcgcttcg cactggacaa tttgggcttg gtctttgcca
     1621 acaaagcgat tctcggcgta gacggaatct ccctaaaaca cgggctgacg gttcccacca
     1681 accc
//
LOCUS       DAOQFT010000552         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_52452_length_1682_cov_6.542717, whole genome shotgun sequence.
ACCESSION   DAOQFT010000552 DAOQFT010000000
VERSION     DAOQFT010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52452_length_1682_cov_6.542717"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..319)
                     /locus_tag="PLR93_11955"
     tRNA            complement(247..319)
                     /locus_tag="PLR93_11955"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(283..285),aa:Glu,seq:ttc)
     gene            complement(373..930)
                     /locus_tag="PLR93_11960"
     CDS             complement(373..930)
                     /locus_tag="PLR93_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27731.1"
                     /translation="MSLDSTSPRTYRFVLADILTSGYRVAGKVAVTTHGAMGFLNDIN
                     HSTLEIHDARMARLHMPTKLVDHFELVRTMKSRIHAVCLPRREDLGPQGIVRGGYMNV
                     VEYQVRLTTSMFEVDGIMELPGRFDFAGLISDGTREFLPIFNATVTAILIPSLRVQSA
                     GVLINRKMVDLVALLNQRVKPETHP"
     gene            complement(945..1232)
                     /locus_tag="PLR93_11965"
     CDS             complement(945..1232)
                     /locus_tag="PLR93_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNH27732.1"
                     /translation="MNELNALTPCNECHAGLLHPRHVTYFTWLGHELITVPHFPAWVC
                     DVCGRREYDERAIQWLNMILDPNAGKPTPSHRNTPPRPRPQTGFRSPTPES"
     gene            complement(1241..>1682)
                     /gene="lepB"
                     /locus_tag="PLR93_11970"
     CDS             complement(1241..>1682)
                     /gene="lepB"
                     /locus_tag="PLR93_11970"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNH27733.1"
                     /translation="VRVDGFSMQPTLQDGEFVLVSKLSYKFGDFQRGDIIVFDFPMNP
                     DEELVKRIIGVPGDHVMVRDSHVYVNEQMLNENYIAQAPLYSGDWIVPDGTLFVLGDN
                     RNNSNDSKDWGLLPQGNVVGKAVLIYWPPKFWKVLDHPEMAVSQ"
BASE COUNT          430 a          461 c          396 g          395 t
ORIGIN      
        1 accttggatg cgcgctaacg ggcgctcccg attctcttat gacttgcgtc tctccgagaa
       61 tgactgggca aagtggattt catcctatgg cggtccgcct cgcactcacc ggcggctctc
      121 tgtaccgttg ctgaactact tatctttgcg cttgtctttg atttcgaact gacgggatta
      181 taccaaaaat gagtctggct ttttaaacca aaaaccgcct cttacgagac ggttgctttt
      241 aggttgtgcc cccacgggga ttcgaacccc ggtcgtagcc ttgaaagggc tgcgtcctag
      301 tccactagac gatgggggca tttcttaagc gggcgagatt ttatcatcca cttcgggatg
      361 cgtcaagaaa ttttagggat gagtttctgg cttcacacgc tggttcaaaa gcgccaccag
      421 gtctaccatt ttgcggttaa tcagcacgcc tgcgctttgc actcgcaagc ttggtatgag
      481 gatcgccgtc actgtagcat tgaaaatcgg caggaattca cgcgtgccat ctgagatcag
      541 cccggcaaaa tcaaagcgac cgggcaactc catgataccg tccacttcaa acatggaagt
      601 cgtcaggcgc acctgatatt ccactacatt catatatccc ccacgcacaa tcccttgcgg
      661 acccaggtct tcacgccgag gtaaacaaac agcatgaatg cggctcttca tcgtgcgtac
      721 caattcgaaa tgatccacta acttggtagg catgtgtaaa cgtgccatac gcgcatcatg
      781 aatttccagc gtcgaatgat tgatatcgtt taagaaaccc atggcgccat gagtggtcac
      841 tgcaaccttg cctgccacac gatacccaga agttaggata tctgccagca caaagcgata
      901 tgtacgagga gaagtgctat caagtgacat ggagaaacct tgagttaaga ttcaggggtc
      961 ggggagcgga accctgtttg aggtcgaggg cgaggcgggg tgttgcgatg cgaaggcgta
     1021 ggcttgccgg cattcgggtc caagatcatg ttcaaccatt ggatcgcgcg ttcatcatac
     1081 tcgcgccgcc cgcacacatc acacacccaa gccgggaaat gcggtacagt gatcaactca
     1141 tgcccgagcc aggtgaagta agtcacatgg cggggatgca gcaaacccgc atggcattcg
     1201 ttacaaggag ttaatgcatt caattcgttc atggcaaacc ctattgtgaa acagccatct
     1261 ccggatgatc taacactttc caaaatttcg ggggccaata gatcaaaaca gccttaccca
     1321 ccacattgcc ttgaggcagc aagccccagt cttttgagtc attcgagtta ttacgattat
     1381 cgcccaacac gaacaaggtt ccatccggca cgatccaatc gccagaatag agcggcgctt
     1441 gggcaatata gttctcgttc aacatctgct cgttcacata tacatggcta tcgcgcacca
     1501 tcacatgatc accgggcacc ccaataatac gcttcaccaa ttcttcatcc gggttcattg
     1561 ggaagtcgaa cacaatgata tcgccgcgtt gaaaatcacc gaacttatag ctcaacttgc
     1621 tcaccaacac gaactcacca tcttgcaacg tgggttgcat actaaagcca tccacccgca
     1681 ca
//
LOCUS       DAOQFT010000553         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_53240_length_1669_cov_5.569393, whole genome shotgun sequence.
ACCESSION   DAOQFT010000553 DAOQFT010000000
VERSION     DAOQFT010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53240_length_1669_cov_5.569393"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..659)
                     /locus_tag="PLR93_11975"
     CDS             complement(78..659)
                     /locus_tag="PLR93_11975"
                     /inference="COORDINATES: protein motif:HMM:NF016581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNH27734.1"
                     /translation="MSTSRQKILTHLKKTRSASAREIARALKLSAPNVRHHLSVLCSD
                     GRVEMTSLHNREGRGRPEKVYSLSAAALGDNLSALAEAVLTVASKTLSMEAVASRILD
                     AKQFAGQPINKRLALLVEVLNEMHYQARWEAGAGGPRVIFGRCPYARVIDGHPEICKM
                     DEAMLKAALEREILPTRRAETQQGLCPFLFEIG"
     gene            861..1175
                     /locus_tag="PLR93_11980"
     CDS             861..1175
                     /locus_tag="PLR93_11980"
                     /inference="COORDINATES: protein motif:HMM:NF022643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HNH27735.1"
                     /translation="MKKNIYLILTIVGFFLPYYFVFKFYTANEMSTSAALAQLVSTDW
                     GALFVADLTISVFAAWTFIYNEAKRLKMNYWWAYPIATMMVGLSFALPLFLYFRERQL
                     EQ"
     gene            1172..1384
                     /gene="iscX"
                     /locus_tag="PLR93_11985"
     CDS             1172..1384
                     /gene="iscX"
                     /locus_tag="PLR93_11985"
                     /inference="COORDINATES: protein motif:HMM:NF016284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNH27736.1"
                     /translation="MSLHWDATFAIAMELRRRHKDVNIEDVTLQQILDWTLELSEFED
                     DPALVNDDILYAIYQDWYEEHIHGRK"
     gene            1371..>1669
                     /locus_tag="PLR93_11990"
     CDS             1371..>1669
                     /locus_tag="PLR93_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNH27737.1"
                     /translation="MEENKYEGLNLPNYNIPEDIQNVVSITTIDRLYNWGRRWSVWPL
                     MFGLACCAIEMIAAQTARYDMARFGMEVMRPTPRQADMMLVSGTVTKKMLPSIIR"
BASE COUNT          426 a          420 c          386 g          437 t
ORIGIN      
        1 aattcttttt gctttgggga aataaaacca cgctgattcg agcgtaattc ttcttctgta
       61 aattcgtaaa acataattca tccgatctca aataaaaatg ggcacaaacc ttgttgtgtc
      121 tctgcacggc gagtgggcaa aatctctcgt tccagtgcag ctttcaacat agcttcatcc
      181 attttacaga tctcggggtg tccatcaatc acccgtgcgt acgggcaacg cccaaatatc
      241 acgcgcggac ctccagcacc cgcctcccat cgggcttgat aatgcatttc attcaacact
      301 tcgactaaca aagccaaacg tttattgata ggttgacctg caaattgctt tgcatccaaa
      361 atgcgactcg ccaccgcttc catacttaac gttttgctgg ctactgtcaa aacagcctcc
      421 gccaatgcgg agaggttatc tcccaacgcc gccgccgaaa gcgaatagac tttctccggt
      481 ctgcccctcc cctcgcggtt gtggaggctg gtcatttcca cgcgcccatc agagcaaagc
      541 acagagaggt ggtggcgcac attcggcgcg gagagtttca aggcgcgtgc aatttcacgg
      601 gcagaagcag agcgagtctt tttgagatgt gtgaggattt tttggcggga agtggacata
      661 ttgtctatac gcaagttttc agagtaggga aagttcaaag aaacagagac aggttcaagt
      721 taagggcgaa gcgagggtgt catcctgatg gagtgattat attcacttct ttatttatgt
      781 aaatatattt ttgcataatt caattgacgt atttccatcc cgtggctata atccgataac
      841 ttgtgaagga ttacaccgat ttgaaaaaga acatctacct cattctcact atagtcgggt
      901 tcttccttcc ttattatttc gttttcaagt tctacactgc caacgaaatg agcacatctg
      961 cagcgctggc tcaattggtc tccaccgatt ggggcgcgct ttttgttgcc gatctgacaa
     1021 tctcagtctt cgccgcgtgg accttcattt ataacgaagc caaacgcttg aagatgaact
     1081 attggtgggc atatccgatt gccaccatga tggttgggct ttcattcgct ttaccgctct
     1141 ttctttattt tcgcgaacga cagcttgaac aatgagcctg cattgggatg ccacatttgc
     1201 catcgcaatg gaattgcgcc gcagacataa agatgtgaac attgaagacg taaccctgca
     1261 acagatatta gattggacgc ttgaactttc ggaatttgag gatgatcccg cgctcgtaaa
     1321 cgacgacatc ctatacgcga tataccaaga ctggtacgag gagcacatcc atggaagaaa
     1381 ataaatacga aggattgaac ctgccgaact acaacattcc ggaggacatt cagaacgtgg
     1441 tttcgatcac cacgatcgac cgcctctaca actggggacg ccgctggtcg gtatggcccc
     1501 tgatgttcgg tttggcatgc tgcgcgatcg aaatgatcgc cgcacaaact gcacggtacg
     1561 atatggcacg cttcggtatg gaagtgatgc gccccacccc gcgtcaggca gacatgatgc
     1621 tggtctctgg caccgtcacc aagaagatgc tgccgtccat catccgctt
//
LOCUS       DAOQFT010000554         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_53861_length_1658_cov_6.093575, whole genome shotgun sequence.
ACCESSION   DAOQFT010000554 DAOQFT010000000
VERSION     DAOQFT010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53861_length_1658_cov_6.093575"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLR93_11995"
     CDS             <1..352
                     /locus_tag="PLR93_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNH27738.1"
                     /translation="EDLKSSELFAIILPGGWAPDKIRRYDVVKKLVKEMDAANKVIGI
                     ICHGGSIAISAGIIKKGQRVTGSTGIKDDLENVGGVWADEAAFREGNQVWGRVVADIP
                     DFNRELVAALVQSL"
     gene            complement(589..1404)
                     /locus_tag="PLR93_12000"
     CDS             complement(589..1404)
                     /locus_tag="PLR93_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH27739.1"
                     /translation="MSKAILVLSDALRYDVAISNMGYLGHMLEYKKASLYKIVGELPS
                     MSRPMYETIHTGMPSSETGIVANSIVRLSEKPNIFKSVREAGMVTAAAAYYWYSELYN
                     RAPYNPIDDREVDDASLNIQHGRFYTTDEYPDIELFNTAGHLVRKYSPDYLLLHPMGM
                     DYHGETYGSDTKEYRNHAIYQDSKLAPLLVEWRERGYTIFVTGDHGINADGGHGGTTP
                     EQRTVPFFVIQPNGQGRGDTGEVISHLQIAPTVLSLLGISIPETMKHAPLALG"
     gene            complement(1456..>1658)
                     /locus_tag="PLR93_12005"
     CDS             complement(1456..>1658)
                     /locus_tag="PLR93_12005"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNH27740.1"
                     /translation="VQKKDNVLDCTIENITFLGSVVRIQVKIGESKFNMDTFNNPFLE
                     LPKIGSKEQVTCSKEAVLVLDE"
BASE COUNT          456 a          382 c          382 g          438 t
ORIGIN      
        1 cgaagacttg aagtccagcg aactctttgc gatcattctc cccggtggtt gggcacccga
       61 taaaatccgc agatatgatg tggtgaagaa actggtcaaa gagatggatg ctgccaataa
      121 agtcattggc attatttgtc acggcggttc aattgccatt tcagcaggca ttatcaagaa
      181 aggtcaacgt gtcactggct caaccggcat caaagacgat ctcgaaaatg ttggcggggt
      241 ctgggctgat gaagccgctt tccgcgaagg caaccaagtt tgggggcggg tggtagcgga
      301 tatccccgat ttcaatcgtg aactggttgc agccttggtt cagtctttat aagggctttt
      361 gaagttctgg gtttcattcc atttgttctg aaagaaaaaa gttcctgcgc caagtaagat
      421 gcatatgccg ctcgggtttt attgccagta attaacgcca acaaaatttg agcagccact
      481 gcgaagccgt aatagagttt catggttgtg cctccaaaaa ttgaccaacc actatacaat
      541 acccggctca tcttaatggc gcaggaacag tgtgttaaga agccgttatt aacccaaagc
      601 caagggagcg tgcttcatcg tttcagggat ggaaataccc agcagactta acactgtggg
      661 tgcgatctgt aaatgagaga taacctcacc cgtatcgcct cttccctgac cgttgggctg
      721 aatcacaaaa aatggaacag tcctctgctc gggcgtagta ccgccatgcc ccccatcagc
      781 attgatgcca tggtcacccg ttacaaaaat tgtgtatcca cgctcgcgcc attccacgag
      841 aagaggcgcg agttttgaat cttgatagat ggcatggtta cgatactcct tagtatcaga
      901 accataggtc tcgccatgat agtccatgcc catagggtga agcaaaagat aatctggcga
      961 atatttgcgg acgagatgtc ccgccgtgtt gaacagctcg atatctgggt attcgtcggt
     1021 ggtatagaaa cgcccatgct gaatgtttag ggaagcatca tccacttccc tatcgtcaat
     1081 gggattgtaa ggcgcacgat tgtacaactc agaataccaa taatacgctg ccgcggcagt
     1141 caccatccct gcttctcgta cagacttaaa aatattcggt ttttcagaaa gcctcacaat
     1201 tgaattcgca acaatgccag tttcactaga aggcataccg gtatggatgg tctcatacat
     1261 cgggcgagac atgcttggca gttcaccaac gatcttgtac aagcttgctt ttttatattc
     1321 caacatgtga cctaaatagc ccatgttgga aatcgctacg tcgtaacgca gggcatcaga
     1381 aagaacaagg atagctttgc tcataaaacc tctataaaga agtaggtcac gctttgagcg
     1441 tgacctacaa tttttttatt cgtccagaac cagaaccgct tcttttgaac aggtaacctg
     1501 ttctttcgaa ccgatctttg gcagttcaag gaacggatta ttgaaggtat ccatattgaa
     1561 tttggattca cctattttta cctgaattcg gaccacagaa ccgaggaagg taatattctc
     1621 aatcgtacag tcgagaacat tatctttctt ttgaacat
//
LOCUS       DAOQFT010000555         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_54418_length_1649_cov_4.008156, whole genome shotgun sequence.
ACCESSION   DAOQFT010000555 DAOQFT010000000
VERSION     DAOQFT010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54418_length_1649_cov_4.008156"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1649
                     /locus_tag="PLR93_12010"
     CDS             16..>1649
                     /locus_tag="PLR93_12010"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNH27741.1"
                     /translation="MNLPQIQVSTPNYTWEWNSASDTFWLHDKNQRLISRAQHKPFII
                     TSPAPLEFTTVTHRIENDQIRITYQGSNPASSLTVVWSFHSDFISLESFLLTLPEDID
                     LVSIVYFPTIEGDSYKPSMYSHYAVVPGLTMSTNISPIVDLHSRLNTTAVLGSGAMRG
                     PGLTQQWGLPAHYFCTFNTSDRWNAIGAKAEQSDAACWGLAALPQGDFRLEIREIGIS
                     PILNLRADLWKHKPDTFGFHFLITFGENYHEAIRKYYQVLLREKFIAPKKHSSKKQEV
                     LLAPQYNTWGVESALALPPEQLTEDLVRDIFGKFKASGMQAKNFVIDDKWEGTYGELK
                     HDSERFPNFEGILKDIRAQGYNIGLWAAFLRCQNPAALGLNESHLLQTHQGRPLWLDH
                     QTSRYGIFDMTQPKVQDVLRQRAKDFIRRYQPDLIKFDFGYELPSLDVAAPQDMKFAG
                     ERLLQKGLEVIVDAMKEENPDLVIMYYGLSPLLIDYYDLHSPDDLVYCIGDYDLESNR
                     RIFFSSLCGELGMPTYSSSGYDWDSAIDIWFDSVASG"
BASE COUNT          418 a          484 c          343 g          404 t
ORIGIN      
        1 atggtgaaaa ttaacgtgaa tctgccccaa attcaagttt cgaccccaaa ctacacttgg
       61 gagtggaata gtgccagcga cacattttgg ctacacgaca aaaaccagcg cctcatctcc
      121 cgtgctcagc ataagccatt cataatcacc tcccccgctc cactggaatt tacaacggtc
      181 acacaccgaa ttgaaaacga ccagattcgc atcacctatc agggatcaaa ccccgcttct
      241 tccttaaccg tagtctggtc tttccactca gactttatct cccttgaatc ttttctcctc
      301 actctccctg aagatataga cctcgttagc attgtgtact tccccaccat tgaaggtgac
      361 tcttacaagc cttccatgta cagccactat gccgttgtcc ccggtctgac catgagcacc
      421 aacatcagcc ccatcgtgga tttgcactcg cgcctcaaca ccaccgccgt gctcggctcc
      481 ggcgccatgc gcggaccagg cttgactcaa caatggggtt tgcctgctca ttacttctgc
      541 accttcaata cgtctgaccg ttggaatgcc atcggcgcga aagccgaaca aagcgacgcc
      601 gcctgctggg gactcgccgc tttaccgcaa ggagacttcc gcttggagat ccgcgagatc
      661 ggcatcagcc ccatcctcaa cctccgcgcg gatttatgga agcacaagcc cgatacattc
      721 ggctttcact tcctcatcac cttcggcgag aattatcatg aagcgattcg taagtattat
      781 caagttttac tgcgtgaaaa atttatcgca cctaaaaagc attcttccaa aaaacaagaa
      841 gttctgctcg ctcctcaata caatacctgg ggtgtagagt ctgcgctagc actgccgcca
      901 gagcaattga ccgaagatct cgtccgtgat attttcggca aattcaaagc ctctggcatg
      961 caagccaaaa acttcgtcat cgatgacaaa tgggaaggca cgtatggtga gctcaaacat
     1021 gactccgaac gcttcccaaa ttttgaaggc atattaaaag acatccgcgc gcaaggatac
     1081 aacatcggct tatgggcagc attcttgcgt tgtcaaaacc ctgctgccct cggactgaat
     1141 gaatcacatc tattgcagac gcaccaaggc agacctctct ggcttgacca ccaaacgtct
     1201 cgttatggca tcttcgacat gacccagccc aaagtgcagg acgtgttacg tcagcgtgcc
     1261 aaagatttca tccgccgtta tcaaccagac ctgatcaaat tcgacttcgg gtatgagctg
     1321 ccctcactcg acgttgcggc tccgcaagat atgaaattcg ccggtgaacg cctgttacaa
     1381 aaagggcttg aagttatcgt tgacgccatg aaagaagaaa acccagacct tgtcatcatg
     1441 tactacggtc tttctccttt actcatcgac tactacgact tgcacagccc agatgacctt
     1501 gtctactgta ttggcgacta tgaccttgaa agcaatcgcc gcatcttctt cagcagtcta
     1561 tgcggtgaat taggtatgcc cacttacagt tcttctggct acgattggga ctctgccatc
     1621 gacatttggt ttgactcggt cgcttcggg
//
LOCUS       DAOQFT010000556         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_54595_length_1646_cov_4.436832, whole genome shotgun sequence.
ACCESSION   DAOQFT010000556 DAOQFT010000000
VERSION     DAOQFT010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54595_length_1646_cov_4.436832"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PLR93_12015"
     CDS             <1..1282
                     /locus_tag="PLR93_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27742.1"
                     /translation="EHEHRASAAWWRYTGRIDVDVMGTQDGSKFPHQLIEDRYFSTNH
                     MMDRGYWVWFIPLGSGNTSVGIVTDDDIHPQNTYGKSYEQALEWLQKNEPKVWEFVRN
                     LPPMDFLSLKRYSYASAQVFSHTRWSCVGEAGFFLDPLYSYGSDFIAVTNTLTVEMIR
                     RERAGSLTEKMVAGYNRLVLDNLYKICLGFYEGMYKIFGHPQIFTPKLTWDTAIYWSW
                     MYQLYVQGMLLNPTEDVFTLGERFRQLNTRVEKLLADWSEKAPPRPLYVLGDMTRMRL
                     MMLLGLDLASRRGPEQMMKIARKNLDRLEEFALVLFWQAVSECYPNHPMLKKKPWVNA
                     WRMHLEPDQWEADGVFEPETASRPLQSMRDNFSGIFGPQSLRERILYNLPYQLLHLGK
                     GFVYYKIVTWIRRIIFVNKPAMWVRWALIKDYPS"
BASE COUNT          408 a          342 c          409 g          487 t
ORIGIN      
        1 tgagcacgaa catcgtgcca gtgcggcttg gtggcgttac actggtcgaa tcgatgtgga
       61 tgtaatgggc acccaggatg gcagtaaatt tccacaccaa ttgatcgagg atcgttactt
      121 ctctaccaat catatgatgg atcggggata ttgggtatgg ttcattccgc ttgggtcggg
      181 aaacaccagt gttggcattg tgaccgatga cgatattcac ccgcagaaca cgtatggcaa
      241 atcctatgaa caggcgctcg aatggttgca aaaaaatgag ccgaaagttt gggaattcgt
      301 ccgtaattta cccccgatgg attttttgag tttaaaacgt tacagctatg catcagccca
      361 ggtgttttca catacccgct ggtcttgtgt gggggaggcg ggtttctttc ttgacccctt
      421 atacagttat ggatcagatt tcattgctgt tacgaataca ctcaccgttg aaatgattcg
      481 ccgtgaacgc gctggttctt tgaccgaaaa aatggttgcg ggctataacc gcctcgtatt
      541 ggataatctt tataagatct gcctgggctt ttatgaagga atgtataaaa tatttggtca
      601 ccctcaaatt ttcacaccaa aacttacctg ggatactgcc atctattggt cgtggatgta
      661 tcaactttac gttcagggga tgttgttaaa cccaacagag gatgtattca cactcggcga
      721 acgtttcaga caattaaata cgcgtgttga aaaattgctc gccgattggt cggaaaaggc
      781 gccgccgcgt ccgctgtatg tgctgggcga tatgactcgt atgcggctca tgatgttgct
      841 ggggttggat ctggcttcgc ggcgcggtcc tgagcagatg atgaaaatcg cccgcaagaa
      901 tctggatcgt ctggaggaat ttgcattggt tttgttttgg caggctgtta gcgagtgtta
      961 tcccaaccat ccaatgttga agaaaaaacc ctgggtaaac gcctggcgta tgcatctcga
     1021 acctgatcag tgggaggcgg atggtgtctt cgagcctgaa actgcttcac gtccattgca
     1081 atccatgcgc gataattttt ccggtatctt tggcccgcaa agtttgcgtg aacgcatctt
     1141 atataacctg ccctaccaat tattgcattt gggaaaaggg tttgtctatt ataaaattgt
     1201 gacttggatc cgcagaatca tatttgtcaa caaaccggcc atgtgggtgc gctgggcttt
     1261 gataaaggat tatccatcgt agtttcaagt taaattgata aaaagaagtt ctttcagtgt
     1321 atgccctata ttcaatttct gttggattgg ctgtcttctg cgagaattaa tcgataatgt
     1381 caacacaagt gacatggaat gagcaggttt gtaatccttt ctgttctttt ccgcaaattc
     1441 tgtgtttgtt tgtgtacaaa atgatttcgc cagttgcctt tgttgcgaag aagctccatt
     1501 tgcagacggc tcggatgggt gggcagaaag tgggatggct tcttggcttt ttgtatgcgg
     1561 tttattgaca ccaattcata aaaattttct caaccatacc taacaactga cctatacctg
     1621 aaataccccg tcgcaagcgg acgggg
//
LOCUS       DAOQFT010000557         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_55256_length_1635_cov_7.037342, whole genome shotgun sequence.
ACCESSION   DAOQFT010000557 DAOQFT010000000
VERSION     DAOQFT010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55256_length_1635_cov_7.037342"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1027)
                     /locus_tag="PLR93_12020"
     CDS             complement(218..1027)
                     /locus_tag="PLR93_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH27743.1"
                     /translation="MEYNFGLTAQDYAKHRAGFPDEFFDRVFADGIVNPTASLLDLGT
                     GTGTLARGFAARGCSVIGMDISSQLLEQAKDLSLQQGLGIEFRLGKAEETGLPDASFD
                     VVSAGQCWHWFDRPRAAQEVKRLLKPGGRVLIAHFDWLPLPGNVVDRTERLIQIYNPK
                     WYERFGNKNGSYPDWFRDLREARFEDVTSFSFDIHVPYTAEAWRGRIRASAGVGGSLS
                     ADEVVRFDSQLKSLLEGLKQEQVKEEARDDFVLYVPHCVFAVYGHKPKVKS"
     gene            complement(1031..1564)
                     /locus_tag="PLR93_12025"
     CDS             complement(1031..1564)
                     /locus_tag="PLR93_12025"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNH27744.1"
                     /translation="MSITPWKILSSRHIYPRFRVDTVEVHNGTTFEPIVFEFRSWANV
                     LALTKNNEVVLVRQYRHGVQETLLELPGGVVNDGESPLDGARRELMEETGYSAEKIIE
                     VGQIYPNPAIQENTIFCYLATDVEPNGEQHFDETEEIEVVLMPLDDVIEMVRQGTFKH
                     ALHVAVLFKALMYLKKI"
BASE COUNT          433 a          446 c          344 g          412 t
ORIGIN      
        1 ttctcggtcg ttttgtagat actcagcata acagcctcct acgtggaatc atcacgtaag
       61 ggcatggcac ctacgtgaaa ggtattaaaa cgttaaatac atgttcaccg ctacttagcg
      121 ggttactatc tgaagaacct acatcgcttt cagtggttag atacatactt ttgaaccgcg
      181 ccaattttaa gccggatgat ggaattacac aacagtttca ggatttgact ttgggtttgt
      241 gcccatagac cgcaaacacg caatgcggaa catacaagac gaaatcatcc cgcgcctctt
      301 ccttgacctg ttcctgcttc aacccttcaa gcagactctt caactgcgag tcaaatctca
      361 ctacttcgtc tgccgaaagg ctgccaccca ccccagcact cgcacggatg cgtccacgcc
      421 aggcttcggc tgtgtagggc acatggatat cgaatgagaa tgaagtgaca tcttcaaagc
      481 gcgcttctcg caagtcacga aaccaatcgg ggtagctacc gttcttattc ccaaagcgtt
      541 cataccattt cgggttatag atttggataa gcctctccgt tcggtcgaca acattcccag
      601 gcagcggaag ccagtcgaag tgagcgatca acactctgcc gcccggctta agcaaacgct
      661 tcacttcctg cgctgctcgc ggacggtcga accaatgcca gcattgcccg gcagagacaa
      721 catcgaatga cgcatcaggt aacccggttt cttcagcctt tcccaaacga aactcgatac
      781 ccaaaccttg ttgaaggctg aggtcttttg cctgctcaag caattgtgaa gaaatatcca
      841 tgccaatgac cgaacagcct cgagcggcga atccgcgggc gagggtgcca gtgcccgttc
      901 cgaggtccag caaagaggcg gtggggttta cgatcccatc agcaaagacg cggtcaaaaa
      961 attcatcagg gaatccagcc cggtgtttgg cataatcttg agctgtaaga ccaaagttat
     1021 attccataga ctatattttt ttcaaataca tcagtgcttt aaacaaaacc gccacatgca
     1081 aagcatgctt gaatgtgcct tgccgcacca tttcgatcac atcgtctaat ggcatgagca
     1141 caacttcgat ctcttcggtc tcgtcaaaat gctgttcacc attcggttcg acatcggttg
     1201 caagatagca aaaaatagta ttctcttgaa tggctgggtt tgggtagatc tgaccaactt
     1261 caataatttt ctctgcagaa taacctgtct cttccattag ctcacgtcgc gccccgtcca
     1321 gcggactctc accatcattt accacgccgc cgggcagttc aagcaacgtt tcttgcacgc
     1381 cgtgccgata ctgccttacc aaaaccactt cgttgttttt ggttaaagcc aatacattcg
     1441 cccagctacg aaattcaaag acgatcggct caaaggttgt accattgtga acctcaaccg
     1501 tatccacccg aaaacgggga tatatgtgcc gcgaactcaa aatcttccaa ggggtaatgg
     1561 acatgtttta cacagcctct ttattgcata gtttttaact tctcaaccaa accattataa
     1621 tttgcccatg cctat
//
LOCUS       DAOQFT010000558         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_56147_length_1621_cov_5.383142, whole genome shotgun sequence.
ACCESSION   DAOQFT010000558 DAOQFT010000000
VERSION     DAOQFT010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56147_length_1621_cov_5.383142"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..714)
                     /locus_tag="PLR93_12030"
     CDS             complement(217..714)
                     /locus_tag="PLR93_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH27745.1"
                     /translation="MDAKERNEKIELYGRGYDLLKAALAEVPAEAMLFKPEPKEWSVY
                     EVIIHIADSESNGALRARKLIVEPGGSIMGYSQPLWADVLKYHEHNLEDALEVTRLAR
                     KTTYQLLKRIPDIVFDTHWIKHPEYEEPYTFDKWIDIYSKHIPGHVEQIENNLKIWSG
                     SRLVK"
     gene            complement(779..1150)
                     /locus_tag="PLR93_12035"
     CDS             complement(779..1150)
                     /locus_tag="PLR93_12035"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH27746.1"
                     /translation="MAKALGVGGIFFKSPDPKRLHAWYAKWLGMKTEEWGLAFYPKDY
                     PANSQTVWSAFDASTTYFDPSKTGFMFNLIVDDLEGALEQVQQGGAEVIGNIEKMEYG
                     SFGWFMDPDGNKVELWEPIKE"
     gene            complement(1163..>1621)
                     /locus_tag="PLR93_12040"
     CDS             complement(1163..>1621)
                     /locus_tag="PLR93_12040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH27747.1"
                     /translation="NSSSMNDGAAATIISSRGYAEKKGLKPLARIVGYAQAAMEPKYL
                     FAAPAFAMPKLLKKIGWTLKDVDLIELNEAFAAQVLADGYALADQGWDWDKVNVNGGA
                     IALGHPLGASGARVLTTLIYALQNRGLKRGIASLCLGGGEAVAMAIELEN"
BASE COUNT          384 a          437 c          334 g          466 t
ORIGIN      
        1 atcatcgaac ccttctgcga tgttatgcat aatcgaacca gcagctccct gaatttggtc
       61 gcgcaatcga aagtcttttc ggaatttttc ctgttcggtc aagtcgtaaa ccagatttgc
      121 caactctcga gcctgttgcc atgctctcac atcttcaaat ttttcaatcg tcataattcc
      181 tcctatgatt attattttaa tcgaacgact aggcggctat ttgaccaagc gactaccaga
      241 ccagattttt aggttatttt caatctgctc aacatgcccc ggaatgtgct ttgaataaat
      301 atcgatccac ttatcaaagg tataaggttc ttcatattca ggatgtttaa tccaatgggt
      361 gtcaaagaca atatcaggaa tacgtttcaa aagttgatag gtggtcttgc gcgccaaacg
      421 tgtcacttca agcgcatctt caagattgtg ttcatggtat ttcaacacat ccgcccaaag
      481 cggctggctg tagcccatga tgctcccgcc cggctcgacg atcaacttgc gcgcacgtaa
      541 tgcgccattt gattcactgt ccgcaatgtg gatgatgact tcgtaaacgc tccactcttt
      601 cggttctggt ttgaagagca ttgcctccgc agggacttct gccaatgccg cttttaataa
      661 gtcatatccg cgaccgtata actctatctt ttcattgcgc tctttagcgt ccatttttat
      721 tgtctcctca taaaatacaa acggagacga acaccactgc ccgtctccgt ttatttggtt
      781 attctttaat gggctcccat aattcaactt tattcccatc cgggtccata aaccatccga
      841 atgagccata ttccattttc tcgatattgc caatcacttc cgcgccacct tgctgaactt
      901 gctcaagtgc gccttccaaa tcatccacga tcaagttgaa catgaaacct gtttttgatg
      961 ggtcaaagta agttgtcgat gcatcaaacg cgctccatac tgtctgactg ttggctggat
     1021 aatcttttgg gtaaaacgcc agcccccatt cttcagtttt catccccaac cacttggcat
     1081 accatgcgtg gagtcgtttg gggtcaggtg acttaaaaaa gatgccgcca acgcctaatg
     1141 ctttcgccat gttggtctac tcctaatttt ccaattcaat cgccatcgct accgcttcac
     1201 cgccaccaag acaaagcgat gcaatgcctc gttttagtcc gcggttttgc aaagcgtaga
     1261 tgagggtggt caacacgcgt gcgccgctgg ctcccagtgg gtgaccgagc gcaattgccc
     1321 cgccattcac gttgaccttg tcccagtccc agccttggtc ggcaagagcg taaccgtccg
     1381 cgaggacttg ggcggcgaag gcttcgttca actcgatcag gtcaacatct ttcaacgtcc
     1441 agccgatttt ctttaacaac tttggcatgg caaatgcagg tgctgcaaaa agatatttcg
     1501 gttccattgc tgcctgggca tagccaacga tccttgcaag cggcttgagc cctttctttt
     1561 cagcatagcc acgcgaagag ataatcgtcg ctgccgcgcc atcgttcatc gacgatgaat
     1621 t
//
LOCUS       DAOQFT010000559         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_56379_length_1617_cov_7.321383, whole genome shotgun sequence.
ACCESSION   DAOQFT010000559 DAOQFT010000000
VERSION     DAOQFT010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56379_length_1617_cov_7.321383"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLR93_12045"
     CDS             <1..544
                     /locus_tag="PLR93_12045"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNH27748.1"
                     /translation="KGSSAMAKNIVLTLTGKDRIGLVDQVTSLVVKRSGNVESSRMAR
                     LGGEFAMLMLVTVSDAEFQNLDKDFQEMRGEGYQVTLMQTEDVSKKYAGWLPYEIEVT
                     GADHEGIIQQISHHLASQGINIESMDTSATPAPMSGTPLFMMKGIVLVPPKLNFNAWS
                     DALEEVGDTLNVSVKIEVVK"
     gene            complement(595..>1617)
                     /locus_tag="PLR93_12050"
     CDS             complement(595..>1617)
                     /locus_tag="PLR93_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH27749.1"
                     /translation="TAISQEIVAELEKSTAAYPELMSLADAEEEFSARRVSAVLVIPA
                     GLEISAIQNGTAELDFRQQPNDMDASIAQRAIQTAIRRVSSAVNAANVAVEEAKARGT
                     FESAEAEQAYFEEALKVAKDLQANSPERITVIEGSTPDKVDYDPRANTSAGQLVTWVF
                     IPLFGISALFAYERQQGTLRRLLTTPTSKAVFLLGTIFGQVVMALIQMSLLILFGMFA
                     LQLKWGQEPLALFILLTCAALAAGAIGTTMGTFIKSEGQASGLSIMAGMVMALLGGCW
                     YPLELFPAAVQNIVKILPTTWAMQGLLDLVLRGGGLTDILLESGVLLGFAVVFFSIGV
                     ARFRYE"
BASE COUNT          431 a          391 c          424 g          371 t
ORIGIN      
        1 gaaagggagt tctgccatgg caaaaaatat tgtattaact ctcacaggca aagatcggat
       61 cgggttggtg gatcaggtga cgagtctggt tgtgaagcgg agtgggaatg tagaatccag
      121 ccgcatggcg cgtttgggtg gcgagtttgc catgttgatg ttggtcacag tatcagacgc
      181 agaatttcag aatctcgata aagattttca agaaatgcgc ggcgaaggct atcaagtcac
      241 cttgatgcag accgaagatg tttctaagaa gtatgcaggt tggctgccct atgagatcga
      301 agtgaccggt gccgaccatg aaggcatcat tcaacaaatt tcacatcatc tggcttcaca
      361 gggtatcaac attgaaagca tggataccag tgcaacgcct gcaccgatga gcggaactcc
      421 gctctttatg atgaaaggga ttgtgcttgt cccgccgaag ttgaacttca atgcctggag
      481 cgatgcattg gaagaagtag gggacacctt gaatgtcagt gtgaagattg aagttgtgaa
      541 ataaaagaaa agcaaacggc ggatgaacat catccgccgt ttttatttta agtgttactc
      601 gtatcgaaat cttgcgaccc caatagagaa gaacacaacc gcaaacccga gcaaaacacc
      661 tgattcaagt aggatatcgg tgagtccgcc gccgcgcaga acgaggtcaa gcaaaccttg
      721 cattgcccaa gtagtaggga gaatcttgac gatgttttgc accgccgcag ggaataactc
      781 caacgggtac caacaaccac ccagcaatgc catcaccatg cctgccataa tactcaaccc
      841 gctggcttga ccttcgcttt tgatgaacgt tcccatggtg gtgccaatcg ctccggcggc
      901 gagcgcggca caggtgagca agataaagag cgcaagcggc tcttgccccc atttgagctg
      961 gagagcgaac atcccaaaca gaatcaacag cgacatttgg atgagtgcca tcactacttg
     1021 accaaagatg gtgccaagca gaaaaacggc tttactggtt ggggtagtga gcaaacgacg
     1081 taatgtccct tgttggcgtt cgtaagcaaa gagcgccgag ataccgaaca aaggaatgaa
     1141 tacccaagtc actaattgcc ctgccgatgt attggcacgc ggatcatagt ccactttatc
     1201 tggtgtgctt ccttcaatga cggtgatacg ttcaggggaa ttggcttgaa ggtctttggc
     1261 aactttcaat gcctcttcaa aataggcttg ctctgcctcc gcggactcaa acgtgccgcg
     1321 cgctttcgcc tcttccacag ctacgtttgc ggcgttgaca gcactgctca cacgtcgaat
     1381 cgccgtttga atggcacgtt gcgcaatgga agcatccatg tcgttgggtt gctggcgaaa
     1441 gtcaagttcg gcagttccgt tttgaatggc agaaatttcc aagccagcag ggatcaccaa
     1501 caccgccgaa acacggcgcg cactgaattc ttcttcggca tctgccagag acattaactc
     1561 ggggtatgcc gcagtggact tttccaactc tgccacgatc tcttgcgaga tggcggt
//
LOCUS       DAOQFT010000560         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_56789_length_1611_cov_7.998072, whole genome shotgun sequence.
ACCESSION   DAOQFT010000560 DAOQFT010000000
VERSION     DAOQFT010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56789_length_1611_cov_7.998072"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..698)
                     /locus_tag="PLR93_12055"
     CDS             complement(18..698)
                     /locus_tag="PLR93_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNH27750.1"
                     /translation="MLVLIAGGGRTGAQLAAQLLQQNYEVRLVEHRKELLARLHHELP
                     TEVIYEGQATDPNVLRQAGLEKANVIVACTNDDAANLVLCYLARTLFKVRRTVARINN
                     PRNSWLFDNNFHVDETINQADVMAHLIQEEMSLGDMMTLLKIRRGRYSLVEEKVPKGA
                     KAIGMQLKDLGLPEQCVIAAIIRDGQVTLPRGTTELEEFDEVLAITDDEGAKQLAHLL
                     EPPDRSVL"
     gene            complement(700..1365)
                     /locus_tag="PLR93_12060"
     CDS             complement(700..1365)
                     /locus_tag="PLR93_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNH27751.1"
                     /translation="MNFIVIGCGRFGAELAYKLFQNGHQVVVVDANPKAFNRLHPDFR
                     GRTVEGDSLSADTLSRASADKATGIAVVTNSDTMNAVIGHTIRVHYPNVEQVIVRNYD
                     PAMRDMLEAFGLQIVSSTAWGAERVQELLIDPSFRAVFSAGNGEVEMYEMYISPKWDG
                     MTISNLLDGCGEIVCSALTRAGRAQLPNGGMKLQKGDILTVSATLEGVKALRAKLQEG
                     KEA"
     gene            complement(1378..>1611)
                     /locus_tag="PLR93_12065"
     CDS             complement(1378..>1611)
                     /locus_tag="PLR93_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27752.1"
                     /translation="EPLLEYIADLAAKRQPGEIITVVVPEFVSENRWTAALHTNTADI
                     LRNQLKNQHGIVITNVPYHVHEAEGDHADHVAH"
BASE COUNT          388 a          414 c          408 g          401 t
ORIGIN      
        1 ttcgggaggt ttttgtttta caacacagaa cgatcaggtg gttccaagag gtgggctaat
       61 tgttttgcgc cctcatcatc ggtaatcgca agcacttcgt caaattcttc aagctcggta
      121 gtaccgcgcg gaagagtcac ctgtccatca cggatgatgg cagcgatgac acattgctca
      181 ggcaaaccca agtctttgag ctgcatacca atggctttgg cacccttggg gaccttctcc
      241 tcgaccagtg agtagcgtcc gcggcggatc ttgagcagtg tcatcatatc gccaagggac
      301 atttcttctt gaatgaggtg agccataacg tccgcttggt tgatggtttc atccacgtgg
      361 aagttgttat caaacagcca gctattgcgc ggattattga tacgagccac cgtgcgacgg
      421 accttgaaaa gggtacgcgc caaatagcat aaaaccaaat ttgcagcatc atcgttggta
      481 caagccacga tcacattggc tttttccaaa ccagcttgac ggagtacgtt cgggtctgtc
      541 gcctgacctt catagataac ttcggtagga agttcatgat gcaagcgagc cagcaattcc
      601 ttgcgatgtt ctacgaggcg cacctcgtaa ttttgttgga gaagttgagc agccagttga
      661 gcgccggtgc gcccgccgcc agcaataagt acaagcatgt tatgcctcct tgccttcctg
      721 caattttgca cgtaaagctt tcacgccttc caaggtcgcg cttacggtga gaatatcacc
      781 cttctgtaat ttcattccgc cattcggcaa ttgagcgcgt ccagcgcgag tcaatgcaga
      841 gcagacaatc tcgccacaac catccagcaa gttggagatg gtcatgccgt cccatttcgg
      901 ggagatgtac atctcataca tttctacttc gccattcccg gcagagaata ccgcacggaa
      961 agatgggtca atcaagagtt cctgaacacg ctccgcaccc catgctgtgg agctgacgat
     1021 ttgcagtcca aaggcttcga gcatatcgcg catggcgggg tcataattgc ggacgatgac
     1081 ttgttcaaca ttcgggtaat gcacccgaat ggtatgtccg ataacggcgt tcattgtgtc
     1141 tgaattggtg accacagcga tgccggtcgc cttatcggcg ctggcgcggg ataaggtgtc
     1201 agcggagaga gagtcacctt ccacggtacg cccgcgaaag tctgggtgga ggcgattgaa
     1261 cgccttgggg ttggcatcca ccacgacaac ttgatgtcca ttttggaaaa gtttatacgc
     1321 cagttcggcg ccaaatcttc cacatccaat aacaatgaaa ttcatagtaa aaactcctta
     1381 gtgggcaaca tgatcggcat ggtcgccctc tgcctcatga acatgataag gcacattggt
     1441 aatcacaatg ccatgttgat tctttaattg attacgcaga atgtctgctg tattcgtgtg
     1501 cagggcagcc gtccaacgat tctcggaaac gaactcgggc accaccactg tgatgatctc
     1561 tccaggttga cgtttcgctg cgaggtctgc aatgtattca agcaacggct c
//
LOCUS       DAOQFT010000561         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_57675_length_1596_cov_6.946139, whole genome shotgun sequence.
ACCESSION   DAOQFT010000561 DAOQFT010000000
VERSION     DAOQFT010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57675_length_1596_cov_6.946139"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLR93_12070"
     CDS             complement(<1..597)
                     /locus_tag="PLR93_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNH27753.1"
                     /translation="MDYKDYYKTLGVDRKASTDEIRTAYRKLAMKYHPDKNPGDKKAE
                     DKFKEINEAYQVLSDEQKRAHYDRLGSAYSNYRSTGGRPGDFRWDDWYQQQAAGQRGS
                     VNMEDLFGGSGGGGFSDFFRTIFGEAMRGQGRGNPFVQDSPGYEQEVSISFQEAFDGT
                     TRQLQKQDGKKLTVRIPAGVKTGSKVRVAGAGPDGSDLY"
     gene            721..1305
                     /locus_tag="PLR93_12075"
     CDS             721..1305
                     /locus_tag="PLR93_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNH27754.1"
                     /translation="MMKSVCVFCGSSDAVHPSYKKAAFQMGVTLAQNGIQIVYGGGKT
                     GLMGELANGALSVGGAVIGVIITSMNTPALAHTGITRMEVTAEMRSRKARMHELSDGF
                     IAMPGGFGTWDELFETLTWAQTGEHEKPVGLFNVNNFYAPLLAAIDHAVAEGFIFSEH
                     RQAISLETDPAKLIESMRNYEHPHDAVKRWMKAE"
     gene            complement(1388..>1596)
                     /locus_tag="PLR93_12080"
     CDS             complement(1388..>1596)
                     /locus_tag="PLR93_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27755.1"
                     /translation="YNFHKPDDRRTLDNGLVLAIEPFFTTGQGHVVEKSDGWSLKTVD
                     NTIAAQFEHTVIITKGEPIILTLP"
BASE COUNT          403 a          401 c          383 g          409 t
ORIGIN      
        1 ataaagatcg cttccatctg gacccgcacc tgccacgcgg acctttgaac cagtcttcac
       61 cccagccgga atgcggacgg tcaatttctt gccatcttgc ttctgtagct gacgggtcgt
      121 gccatcaaag gcttcttgaa aactgatgct aacttcttgc tcataccccg gtgaatcttg
      181 tacgaatgga ttcccacgcc cttgtccgcg catggcttcg ccaaagatgg tgcggaagaa
      241 gtcagaaaat ccgccgccac ccgaaccgcc gaacaagtct tccatattca cactaccgcg
      301 ctgacccgcc gcctgttgtt gataccaatc atcccaacgg aaatcacccg gtcgcccacc
      361 agtggaacga tagttggagt aggcgctgcc aaggcggtcg taatgagcgc gtttttgttc
      421 atcgctcaaa acctgataag cttcgttgat ctccttgaac ttatcttccg cctttttatc
      481 gccagggttt ttgtctggat gatatttcat ggcgagttta cgatatgccg tgcgaatctc
      541 atccgtgctg gctttgcggt ctacaccaag ggttttatag tagtctttat agtccatgac
      601 gctattttca tcccgtcaag ggacagggtc aagcgtccaa aggttgcact tacataactc
      661 taacctgtct gtagtgtggt agagtcagcg aaaattattc taagaagaca aaatgagggc
      721 atgatgaaat cagtttgtgt attttgtggg tcttcagatg ccgttcaccc ctcctacaaa
      781 aaggcggcat ttcaaatggg cgtgaccctt gcccagaatg gcattcaaat tgtctatggt
      841 ggcggcaaga ccgggctgat gggcgaacta gcgaacgggg ctttatccgt aggtggcgct
      901 gtgatcggcg taattatcac ctcaatgaac acccccgcat tagcgcatac cggcatcacc
      961 cgcatggaag taaccgctga aatgcgatca cgcaaggctc gtatgcatga actatcggat
     1021 ggctttattg caatgcccgg cggttttggc acgtgggatg aactcttcga aaccctcaca
     1081 tgggcacaaa ccggcgaaca tgaaaaacca gttgggctgt tcaatgtgaa taacttttac
     1141 gcgcccctac tcgctgccat agaccatgcc gttgcagaag gctttatttt ttcagaacat
     1201 cggcaggcaa tttcactgga gaccgacccc gctaaactga tagagtctat gcggaattac
     1261 gaacacccgc atgatgcagt gaaacgatgg atgaaggctg aataaggatc ttggattaaa
     1321 caatttacgc atcaggtaat acgagatttg tattgcctga tgcgtaatga tttaacaagc
     1381 gtgttttcta tggcaacgtc aaaataatcg gctcgccttt ggtgatgatg acggtatgct
     1441 caaattgagc cgcaatcgta ttatccacag ttttcaaaga ccagccatca gacttttcga
     1501 caacgtgtcc ctgcccggtg gtaaaaaaag gctcaatggc aaggaccaag ccattatcca
     1561 gtgtgcggcg gtcatctggc ttatgaaagt tataga
//
LOCUS       DAOQFT010000562         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_57988_length_1591_cov_3.972005, whole genome shotgun sequence.
ACCESSION   DAOQFT010000562 DAOQFT010000000
VERSION     DAOQFT010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57988_length_1591_cov_3.972005"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLR93_12085"
     CDS             complement(<1..493)
                     /locus_tag="PLR93_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH27756.1"
                     /translation="MIQIQLSNITLVLGAKRIFENLNWEIQRGQRIGLIGANGAGKSS
                     LFKLIEGEHAPELGGSITRARLITTGYLPQHPQLDPRLTALDAAMQGNPRVGEVHAEL
                     ERVEASLGDPEVYGNEKKLERALERQHQLIEEYHVLGGDSYPERVKGILLGLGLAQSE
                     LTKP"
     gene            complement(732..>1591)
                     /locus_tag="PLR93_12090"
     CDS             complement(732..>1591)
                     /locus_tag="PLR93_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNH27757.1"
                     /translation="NAADIRIVLDAMELFYNGRDSFTHVLLASGDGDFTELVHRLRAN
                     GKIVIGLGISGTTADYLVSACDKFVYYDKWQGVGKFKKETPSNGGQKQQPAKQNNPPA
                     QKQEKPQEKAQPQEKPAPKPAAPISPEKRLERYLNILSSHKIRMVPSTNRPLVIYKIY
                     DIVKEDAGITFNQLKEKVQAIFANTKVEPQLALDTAHQLFHTFCFEFDQSNDRIMDRK
                     MLIPEDTKSPADLLNKCDQKLLHLIAGDLGASEVFDKETAAKILYGGVRNPKVLDHIQ
                     ELLDAETQV"
BASE COUNT          361 a          382 c          410 g          438 t
ORIGIN      
        1 ggggtttggt caattcgctt tgagcgagtc ccagcccgag caggattccc ttaacccgtt
       61 ccgggtatga gtcaccgccg aggacgtgat actcttcaat gagttgatgc tgtcgttcca
      121 gtgcgcgctc caacttcttt tcattgccat agacttcagg gtcacccaaa ctggcttcga
      181 ctctttcaag ctccgcatgc acctccccta cacgcgggtt gccttgcatc gcggcgtcga
      241 gcgctgtcag ccttgggtca agttggggat gttgaggcag gtatcccgtt gtaatgaggc
      301 gggcgcgggt aatgcttccg cccagttcgg gggcatgttc cccttcaata agtttgaata
      361 gcgacgactt ccccgcacca ttcgcaccga taagaccaat acgctgtccg cgctggatct
      421 cccagttgag attttcaaag atgcgcttgg caccgaggac gagtgtgatg ttggagagtt
      481 gaatttggat catagttttt tccggtttgt aggggcgacc cttcaaggtt tgtcaaagtg
      541 tttgatcaat ctaggcgggt tgcccttacg atattattaa acaaaaacgc cgcaggcaat
      601 gcctgcggcg tatgaaagta aaaactttca gcgtacaggc gcaatacgac aaggaacggc
      661 gttatcaaag ccattgatcg ccattgcaaa aaggggtttg cctacgctga tgatgtaaag
      721 catatttgcg attatacctg agtttctgcg tcgagcaatt cttgaatatg atcaagcacc
      781 ttggggttgc gcactccgcc ataaagaatc ttagcggcag tttctttatc aaagacttcc
      841 gaagccccga ggtcaccagc gatcaggtgc agcaacttct ggtcacattt attgagcaga
      901 tcagcgggag attttgtatc ttcaggaatc agcattttgc gatccatgat gcggtcattg
      961 ctttggtcga actcgaagca gaaggtgtgg aacaactgat gtgcagtatc caacgcgagt
     1021 tgcggctcga cctttgtatt ggcaaagatt gcttgcactt tttcttttag ctgattgaag
     1081 gtaataccgg catcttcttt gacaatatcg taaatcttgt aaatcaccag cggacggttc
     1141 gtgctcggca ccatgcgaat cttgtgtgaa gaaagaatgt tgagataacg ctccaggcgt
     1201 ttttctggtg agattggcgc ggcaggtttg ggtgcgggtt tttcctgtgg ctgtgctttc
     1261 tcttgcggtt tctcttgttt ctgcgcaggt gggttattct gtttcgctgg ctgttgtttt
     1321 tgcccgccgt tcgaaggggt ttccttcttg aacttaccca caccttgcca tttgtcgtaa
     1381 taaacaaatt tatcgcaagc actgacgaga taatcagccg tggtgccact gatgcccaac
     1441 ccaatgacga tcttgccatt ggcacgcaag cgatgaacca actctgtaaa atcgccatcg
     1501 ccagaagcta acaaaacatg cgtaaaggaa tctcgcccgt tgtaaaaaag ctccatggca
     1561 tcgagcacga tgcgaatatc ggcggcgttc t
//
LOCUS       DAOQFT010000563         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_58124_length_1589_cov_7.727510, whole genome shotgun sequence.
ACCESSION   DAOQFT010000563 DAOQFT010000000
VERSION     DAOQFT010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58124_length_1589_cov_7.727510"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1422
                     /locus_tag="PLR93_12095"
     CDS             190..1422
                     /locus_tag="PLR93_12095"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH27758.1"
                     /translation="MHITILALGSRGDVQPYAALGKGFKADGHQVRFITFEDFAPLIT
                     ESELDFHSIQGNAKELVANAGADLPALVRSFGSLAEGYARDLSAPYLGETDLIINQLP
                     AGLFGFDLAEKYDVPMVQAAVIPLARTRGFPLMGLPKLPLPGYNKATYYLGEQIAWQM
                     FRTVINRWRKKTLNLPPLPLLGYFDQLGTRRFPIVNGFSQYVVARPADWNEYVHITGY
                     WFPEDRNWEPPHDLSAFIEAGSPPVFIGFGSMPVKDPQRITNIILDSLKQSGQRGILH
                     MGWGGLGNQSLPDHVFKINYAPYEWLFPRMAMSIHHGGSGTTAFGLRAGVPSCVVSFV
                     FDQHYWGERIAQLGAGPRPIRFKELTTERLREAIQLGVSDPKMRQKATELGRKIQTEN
                     GIKNALKVFEKITYRDEN"
BASE COUNT          391 a          378 c          391 g          429 t
ORIGIN      
        1 cctatcacga gcgctacaag aacagcaacg ccaccaccga agattttgtc gctgtggccg
       61 aggagatcag cggacaggaa ttgggcgagt tcttcaacaa ttggttgtac agcggctcac
      121 ttccgcctat tccagagttg ggattgggta ctgaataaat agtcaggtgc ttcactcaag
      181 atattttcca tgcacataac gattcttgca ctaggttcac gcggtgatgt tcagccctat
      241 gctgccctcg gcaaagggtt caaagctgac ggccatcaag tccgttttat tacctttgaa
      301 gactttgcac cacttataac cgaaagcgag ctggactttc actcaattca aggaaatgcg
      361 aaggaattgg tcgccaatgc aggagcagac ctgcctgccc ttgtacgttc ctttggatct
      421 ctggcagaag gttacgctcg tgatttgtct gcaccatacc tgggtgaaac agacctcatt
      481 attaatcagc ttccagcagg attattcggt tttgacttgg cggaaaaata cgatgtgccc
      541 atggtacaag cagccgtaat tccattggct cggacacggg gatttcctct tatgggtctt
      601 ccaaaacttc ccttgccggg ctataacaaa gcaacttatt atttgggcga acagatagcg
      661 tggcaaatgt ttcgtactgt aatcaatcgt tggcgtaaaa aaacgctcaa cctgcctccg
      721 ttgccgctgc tgggttactt tgatcaattg ggtacgaggc ggtttccaat cgtgaacggg
      781 tttagtcaat atgtggtcgc gcgtcccgcc gattggaacg aatatgtcca tatcacgggt
      841 tattggtttc cagaagatag aaattgggag ccaccgcatg atttatcagc attcattgaa
      901 gcaggcagtc cgcctgtgtt tattggtttc ggaagtatgc cggtaaaaga tccgcagcgc
      961 atcaccaaca tcattcttga ttccttaaaa cagagcgggc aacgtggcat tttacatatg
     1021 ggctggggcg gccttggaaa tcagtcgtta cctgatcatg ttttcaagat caattatgcc
     1081 ccgtatgagt ggttgttccc acgaatggca atgtcgattc atcatggtgg ctcaggaaca
     1141 actgcctttg gtcttcgcgc tggtgtgcca tcctgtgttg tgtcgtttgt gtttgaccag
     1201 cactattggg gggaacgcat cgcccagttg ggggcgggtc ccaggcctat tcgatttaag
     1261 gaattgacta ccgaacgact gcgggaagct attcaactcg gtgttagcga tcctaagatg
     1321 cgccaaaaag ccaccgaact tgggcgcaag atccaaacag aaaatggaat taaaaacgct
     1381 ctgaaagtat tcgagaaaat tacctatcgt gacgagaatt aaaactgtga tgaccagcca
     1441 tatccgcgtt tacctgaaaa agaattggct tctgctcctc ctttctgcgc tgtttggcgt
     1501 atgggcactg gccttcattt accattcctc ctacgtggcc agcgacggac gcctgtattt
     1561 caaccttttt gatgatgcca tgatctcca
//
LOCUS       DAOQFT010000564         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_58182_length_1588_cov_8.107632, whole genome shotgun sequence.
ACCESSION   DAOQFT010000564 DAOQFT010000000
VERSION     DAOQFT010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58182_length_1588_cov_8.107632"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..>1588)
                     /gene="fusA"
                     /locus_tag="PLR93_12100"
     CDS             complement(80..>1588)
                     /gene="fusA"
                     /locus_tag="PLR93_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNH27759.1"
                     /translation="DLGKEPKVTEIPADLKAQAEEARSKMVEKIAELDDELTMKFLEA
                     EEISVDELKAALRKGVIANKATPVFCGSSLKNKGVQAMLDAVVDYLPSPIDKPTISAS
                     EPGNPENTFELPTVDDAPLSALVFKIVTDPYVGRLAYIRVYSGVLSQGQTIQNSTKGK
                     KERIGRLLKMHADRREDITEIRAGDIGAVLGFKDSFTGDTLCDNKSLVLESISFPEPV
                     ISIAIEPKSTGDQEKMGEALRKLAEEDPTFRVNSDESTGQTVIRGMGELHLDIIVDRL
                     LREFKVQANVGTPRVAYRESITKPVKEVNYKYAKQSGGKGQYGHVVFALEPGERGSGI
                     IFENKIVGGSIPKEYINPVEKGFREAAESGVLAGYPVVDLKITLFDGSFHEVDSSEMA
                     FKLAASMGFKEGMQKGNPILLEPMMKVEVVAPEEYLGDVMGQVNSRRGLIQGMDVRPG
                     NAQAVRAMVPLGEMFGYATQLRSATQGRGVFSMEFDHYAPVAQSVAEEILKS"
BASE COUNT          420 a          416 c          349 g          403 t
ORIGIN      
        1 catcgttccc acattcaagt gcggcttgct tctgtcaaac ttccctttcg ccatgataat
       61 actccttggg taataaccgt tacgatttca aaatttcttc agcaaccgac tgcgcaactg
      121 gagcgtagtg gtcaaattcc atactaaata cgccgcgtcc ttgggtggcg gagcgaagct
      181 gagtcgcata cccgaacatt tcacccaatg gaaccatggc acggacagcc tgagcgttac
      241 cagggcgaac atccatacct tggatcaaac cacggcggct gttaacctgt cccatcacgt
      301 cgccaagata ttcttccggc gcaactactt caaccttcat catcggttca agcaaaatag
      361 gattgccctt ctgcatacct tctttgaagc ccatggaagc ggcaagtttg aatgccattt
      421 cgctggagtc aacttcatgg aaggaaccat cgaagagagt gatcttcaag tcaacaactg
      481 gatatccggc aagtacgccg ctttcggcag cttcacggaa acctttttcg accgggttga
      541 tatattcctt cggaatggaa ccacccacga tcttgttttc gaagataata ccgctaccgc
      601 gttcaccagg ctcgagggca aatacaacgt gaccatattg acccttacca ccggattgct
      661 tggcatactt gtagttaact tccttgaccg gcttggtaat ggactcgcgg tacgccacac
      721 gcggggtacc gacgttcgct tgcactttga attcgcgcaa gagacggtca acgataatat
      781 caagatgaag ctcacccata ccgcggatca cggtctgacc ggtgctctca tctgaattaa
      841 cacggaatgt gggatcttct tcagcaagtt tacgaagcgc ctcgcccatc ttttcctgat
      901 cgccagtact cttcggctca atagcaatag aaataacagg ctcggggaac gaaatgcttt
      961 caagaacaag tgacttatta tcgcaaaggg tatcaccagt gaagctgtct ttgaagccga
     1021 gaacagcgcc aatgtcgcca gcgcgaatct cagtgatgtc ttcacgacgg tcagcatgca
     1081 tctttaacaa acgaccaatg cgttctttct tacccttggt gctattctga atagtctgac
     1141 cttggctcaa aacacccgag taaacacgaa tatacgcaag gcgtcctaca taagggtcgg
     1201 tcacgatctt gaaaaccaaa gcgctcaaag gcgcatcatc aactgttgga agctcaaaag
     1261 tgttttcagg attcccaggt tcagatgcgg aaatggtagg cttgtctatc ggagagggaa
     1321 gatagtccac cacagcgtca agcattgcct gaacaccttt attctttaat gaagaaccgc
     1381 aaaaaacagg cgtagcctta ttggcgatca cacctttacg aagtgctgcc ttgagttcat
     1441 caactgaaat ttcctccgct tcaaggaatt tcatggttaa ttcatcgtcg agttcggcaa
     1501 tcttctcaac catcttggag cgggcttctt cagcctgagc ttttaaatca gcaggaattt
     1561 cagtaacttt aggttcttta ccgagatc
//
LOCUS       DAOQFT010000565         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_59354_length_1570_cov_3.939274, whole genome shotgun sequence.
ACCESSION   DAOQFT010000565 DAOQFT010000000
VERSION     DAOQFT010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59354_length_1570_cov_3.939274"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1421)
                     /locus_tag="PLR93_12105"
     CDS             complement(252..1421)
                     /locus_tag="PLR93_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNH27760.1"
                     /translation="MTDLPIYPLTNEHKMLRDAARDFAQKEIAPIAAEFDESGDFPHS
                     TIKKMGELGFMGIEVPEQYGGAGMDPLAYVLALEEICKVDASHGVIMSVNNSLYCHGI
                     LKFGTEEQKKKFITPIASGQAIGAYSLTEPQSGSDAGTMRSRATRDGDFYILNGRKSW
                     VTSGPVANYFVVFMMTDPEKKQKGVTAFLVDGKTPGLTRGKKEPKLGIRASATSELIF
                     EDCRVPVENRLGAEGEGFKIAMTVLDAGRIGIATQALGIAEAAYEAARQYAGQREAFG
                     QPIGQFQGTGFKLADMKTRIEASRLLIYNAALAKEKAKESDGRYSLEASMAKLFASET
                     AMYVTHQAVQIHGGMGYSKELPVERYFRDAKITEIYEGTSEIQRLVISRLELGSK"
BASE COUNT          382 a          373 c          378 g          437 t
ORIGIN      
        1 taagccgggc caaccgttgc gcacaaaaac atcgactcga ttaagagccg cggctcttaa
       61 tcgaactaat gcttcgccag ctttgggctg tggagtaggg aagtcggtat attctaagac
      121 ttcaggtccg ccatgagtgt ggaaaaggat agctttcatg ggtgtgccgt gtatcagaga
      181 aaagcaggga tagcacaaag gcgggcaact atccctgaag cagaatgaga tggaagatta
      241 ttcatgaaat gttatttaga gcctaattcg agtctcgaaa taaccaatcg ttgaatttcg
      301 ctggtgcctt cataaatttc ggtaatcttt gcatcacgga agtaacgctc tacgggtaat
      361 tctttgctat aacccatgcc accgtgaatt tgcacagctt gatgtgtcac atacatggcg
      421 gtttcagaag cgaacagttt tgccattgaa gcttcgaggg agtaacgccc atctgactct
      481 tttgcctttt ctttcgccag cgcagcgttg tagatcaata aacgggatgc ctcgatgcgg
      541 gtcttcatat ctgcgagctt gaaacctgtc ccttggaatt gaccaatggg ctgaccaaac
      601 gcttcacgct gacccgcata ctggcgtgcg gcttcatagg cggcttctgc aatcccgagc
      661 gcttgggttg caattccaat acgaccagca tccaagacgg tcatggcgat cttgaaacct
      721 tcgccttcag ctccaagccg attttcgaca gggacacggc aatcttcaaa gatcaactca
      781 ctggttgccg aagcgcggat gccaagtttc ggctccttct ttccgcgtgt cagaccgggg
      841 gttttgccat ccaccagaaa ggctgtcaca cctttttgtt ttttctcagg atcggtcatc
      901 atgaacacga cgaagtaatt tgccacaggt ccgctggtga cccaggattt gcgtccattc
      961 aaaatataga aatcaccatc gcgagtcgca cggctgcgca tcgtcccagc gtctgagccg
     1021 ctttggggtt ctgtcaaaga ataagcgccg atagcttgtc cagaagcgat tggggtgatg
     1081 aacttctttt tctgttcttc ggttccaaac tttagaatgc catggcaata caaggaatta
     1141 ttgactgaca taatcacgcc gtgagaggca tctactttgc agatttcttc gagggcgagg
     1201 acataggcga gagggtccat gcctgcgcct ccatattgtt ctggcacttc aatacccatg
     1261 aaacccaatt cgcccatctt tttgatggtg ctgtgtggaa aatctccgct ttcatcgaat
     1321 tcggctgcaa ttggggcgat ttctttttgc gcaaaatcgc gggcagcatc gcggagcatt
     1381 ttatgttcat ttgtaagtgg gtatatggga agatcagtca tctaaaagcc tccgaatggt
     1441 atttctgaat tatacccttt gtattattcg gaaatttgtg attgacgttt aatcttgctc
     1501 ttaactataa tatgagccta atccccgtat ccctgtcatt ggatgtgggg aattctttcc
     1561 taatgaccta
//
LOCUS       DAOQFT010000566         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_59554_length_1567_cov_4.566799, whole genome shotgun sequence.
ACCESSION   DAOQFT010000566 DAOQFT010000000
VERSION     DAOQFT010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59554_length_1567_cov_4.566799"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="PLR93_12110"
     CDS             complement(<1..1281)
                     /locus_tag="PLR93_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH27761.1"
                     /translation="MNADPRRLPQVLYIADSDESRSLVRRLLAYTFVVLEAANPLDGL
                     GLAEETRPNLILLDTNVAQMTSSEAATRLKKMLSGTPIVVVSGDTSNKARARALAAGA
                     VGFIPKPIGDDFEQLVIEYLNGRTDELENAEQYLREYQLELAEKIEDQTRQLTNTVER
                     NKYLLSQNQKMFAMLERRHKLLETAARVGQMVTSILDMTELLRHTVNIICSEFHFYYS
                     GIFLISDDRKWAVLRAGFALAGRKMLDADYKLPIDDKSMIGRSILTQQAQIALDVAGE
                     ESRFKNPYLPNTRSEMALPLIVNSISVGAVTIQSSELNAFNEEDITSLQAMADQVAIA
                     INNANLMKDLASANAELLRTKTYEAIATATGEAIHWVGNKAAPIPGSVHRVREDVQYL
                     LALVAQLKESELDNATLQAAVETVREEAEAAGLQC"
     gene            complement(1292..>1567)
                     /locus_tag="PLR93_12115"
     CDS             complement(1292..>1567)
                     /locus_tag="PLR93_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH27762.1"
                     /translation="VEVVLQKQRSKETVLSRLGPNEFFGDIELLRGGKSIANVRAGSE
                     PVEVVIIPREDFTRVMDESPITVEAISKIVQRRLEQHRMVDPRKSRS"
BASE COUNT          412 a          406 c          325 g          424 t
ORIGIN      
        1 acattgcaag cctgccgctt ccgcctcttc ccggaccgtt tcaacggcag cttgcaatgt
       61 ggcattatcc aactctgatt ccttcaactg cgcaaccaaa gccaataaat attgaacatc
      121 ttcacggaca cgatgcacac ttccaggaat cggcgctgcc ttattcccaa cccagtgaat
      181 tgcttccccg gttgctgtag caattgcctc atacgtcttt gtgcgcaaca attcggcatt
      241 tgcagatgcc aggtctttca tgagattggc attgttgata gcaattgcaa cctgatctgc
      301 catagcttgt aaagacgtaa tatcttcttc attgaaagca ttaagctcac tactttgaat
      361 agtcactgca ccgacagaga tcgaattgac aataagtggc aatgccatct ctgagcgagt
      421 gttcggcaaa tacgggttct tgaagcgtga ctcctcaccc gccacatcca atgcaatctg
      481 agcttgttgt gtcaagatcg agcgtccgat catggattta tcatcaatcg gcaatttgta
      541 atcggcgtcc agcatcttgc gcccagccaa tgcaaaccct gctcgtagaa ccgcccactt
      601 acggtcgtct gagatcagga aaattccaga ataatagaaa tggaactcac tacaaataat
      661 attgacggtg tgccgaagta gttctgtcat atccagaata gatgtgacca tctgccctac
      721 tcgtgccgcc gtttccaata acttatgtct gcgctcaagc atggcaaaca tcttctggtt
      781 ctgtgaaagc agatatttat tccgttcaac cgtattggtc aattgacgag tttggtcttc
      841 aatcttttcg gctaattcaa gttgatattc acgcaaatac tgctcggcat tttctaattc
      901 atcggttctg ccgttcaggt attcaataac caattgttca aagtcatcgc caattggctt
      961 gggaatgaag cccaccgctc cagccgccaa agcccgcgca cgtgccttat tcgatgtatc
     1021 tccagaaaca accacaatgg gagtaccaga caacattttc ttaaggcgag ttgctgcctc
     1081 actgcttgtc atctgagcaa cattggtatc tagcaaaatc aaatttggac gggtttcttc
     1141 agccagtcct aacccatcta acggattcgc agcctctaac acaacaaagg tatatgcaag
     1201 caaacgccgc acaagcgaac gggattcgtc gctatcggcg atatataaaa cttgcgggag
     1261 tctgcgaggg tcagcgttca taggagcaaa tctacgagcg actctttctt gggtctacca
     1321 tgcgatgttg ttccaaacgt ctctgaacga tcttgctgat ggcttctacg gtgataggag
     1381 attcatccat aacacgcgtg aaatcttcac gtggaatgat tacgacctca accggctcgc
     1441 ttcctgcacg tacattcgca atcgatttgc cgccgcgtaa gagttcaata tcaccaaaaa
     1501 attcattagg tcccaagcgc gaaagaacgg tttctttgct gcgttgcttt tgtaaaacga
     1561 cttctac
//
LOCUS       DAOQFT010000567         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_59884_length_1562_cov_5.921699, whole genome shotgun sequence.
ACCESSION   DAOQFT010000567 DAOQFT010000000
VERSION     DAOQFT010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59884_length_1562_cov_5.921699"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLR93_12120"
     CDS             complement(<1..701)
                     /locus_tag="PLR93_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNH27763.1"
                     /translation="MIYLDYAATTPVDERVLAKMMPYFRDSFGNPSSIHRFGQKAEAA
                     VDEAREKVASVLGCRPDEVIFTSGGSEADNLALRGAMVANKASSKWLLTAKTEHPAVS
                     KTAIQLEKEYGFPLEWLDVDEHGVVTVESLSKAICNDTTMASVMYGNNEIGTINPIAD
                     LAKVAKSNNVLFHTDAVQAAAYLDVNVTKLGVDLMSLGGHKFYGPKGVGALYVRKGTK
                     LVPHLTGGSQESSLRA"
     gene            764..>1562
                     /locus_tag="PLR93_12125"
     CDS             764..>1562
                     /locus_tag="PLR93_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27764.1"
                     /translation="MHNRTQWLKIAGGALGTTAVLLMVFANQLGLESFPTWGVRRVTI
                     LLIGILLLSVSLFYQEDNFIGKFIHSADGPLYIALLVLNAGIFLIYIWYVSTGLMKTF
                     TSETNYFDLQANAFAKGQLALDVQPDPALLAFKDESLYEPENRIGIPVLWDATLYQGK
                     YYLYWGPAPALLLAAIKSFYTPDIGDRLLVLIFLTGTLVFLNLIILDLRKRYFPNIPN
                     WAVLIAIAFAGMINPMLYVLVEGRIYEAAIVAAQFFLIGGFYFLLPAF"
BASE COUNT          375 a          398 c          340 g          449 t
ORIGIN      
        1 gcccgcaagc tagactcctg gcttccgccc gtaagatgcg gcaccaactt cgtccctttg
       61 cggacgtaca acgcccccac ccctttcggt ccgtagaatt tatgccctcc caacgacatc
      121 aagtccacac cgagtttggt cacatttaca tcaagatatg ccgcggcttg gacagcatct
      181 gtatggaata agacattatt cgacttggca acttttgcca agtctgcaat cgggttgatg
      241 gtgccaattt cattatttcc atacatcaca gacgccatcg tcgtgtcatt gcaaatcgcc
      301 tttgaaagcg attcgaccgt cacaacacca tgttcatcta catccaacca ctctaacgga
      361 aaaccatatt ccttttcaag ttgtatcgcc gttttgctta cagcggggtg ttcggtcttg
      421 gcagttagca accacttgga cgaggcttta ttcgccacca tggcgccgcg caaggcgagg
      481 ttatccgctt ctgagccgcc agaggtgaaa atgacctcat cggggcggca tcctaaaacc
      541 gaggcaacct tctcgcgcgc ctcatccact gcggcttccg ctttttgccc aaaacggtga
      601 attgacgagg ggtttccaaa cgaatcgcgg aaatatggca tcattttagc gagcacacgc
      661 tcgtctacgg gtgtggtcgc ggcataatca agataaatca tgactgggat tatagcaaag
      721 gttaatcata aaaagaccgg tctcaaaatg ataaaatcat tccatgcaca atcgtactca
      781 atggctaaaa atcgcgggtg gggcactcgg aactaccgct gttcttttga tggtctttgc
      841 aaatcaattg ggattagaaa gcttccctac ttggggggta cgtcgcgtca caattttatt
      901 gatcggcatt ctgctgctct cagtttctct tttctatcaa gaagataatt tcatcggcaa
      961 gttcattcat tcagcagatg gtccgcttta tattgcgctt cttgttctca atgcaggcat
     1021 ctttctcatt tacatttggt acgtcagcac cgggttgatg aaaaccttta ccagcgaaac
     1081 caattatttt gacctgcaag ccaatgcttt tgccaaaggt caactggcgt tggatgttca
     1141 gcccgaccct gctctcttgg cattcaaaga cgaaagccta tacgagccag aaaatcgcat
     1201 cggcattcca gtcttatggg atgcgactct ctatcaaggc aaatactatt tatattgggg
     1261 tccggcacct gcgcttttat tagctgccat caaatccttt tacacgcccg atattggcga
     1321 ccgtctgcta gtgttgattt tcctcactgg caccttggtc ttccttaact tgattattct
     1381 agacctgcga aaaagatatt ttcctaacat tccaaattgg gcagttctta ttgcgattgc
     1441 atttgcaggg atgatcaacc cgatgttgta tgtgttggtg gaagggcgca tctacgaagc
     1501 tgctattgtg gcggcacaat tctttctaat tggtgggttt tattttcttc tacccgcatt
     1561 tg
//
LOCUS       DAOQFT010000568         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_60460_length_1554_cov_4.842562, whole genome shotgun sequence.
ACCESSION   DAOQFT010000568 DAOQFT010000000
VERSION     DAOQFT010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60460_length_1554_cov_4.842562"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLR93_12130"
     CDS             <1..342
                     /locus_tag="PLR93_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27765.1"
                     /translation="SALKIFQKAESYFGEVRREDRLRIAKWTVARCLRSMNRIDEALS
                     KQMALKAEFDSIGESDGYVDEEIGECLLLLKRENEAKPYFAKAYELLSQDEWMMANEP
                     ERMERMKELCG"
     gene            complement(421..>1554)
                     /locus_tag="PLR93_12135"
     CDS             complement(421..>1554)
                     /locus_tag="PLR93_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNH27766.1"
                     /translation="RRDYQEWFIGRCFDAMNSTEVLSPVEPERTEQMDSAVLINIHSV
                     NETAPHRNVGLVIETRPDEINPDEIKWLRHLGVTKVQMGAQSFNDHILDINKRGHDVE
                     TTRKAVALLRAAGFKIVLHWMPNLLGATPQSDREDFARLWNDFCPDEIKIYPNQLLAN
                     AELYEYWQRGEFQPYTTQELVDLIADIKPSIPRYCRVNRVIRDIPGNNVVEGNRRTSL
                     RQDVHDEMKKRGTKCQCIRCREVKGKTVELSSLALNDLVYQAGKAEEHFISFNTAEDG
                     LAGFIRLSLPSKDSPETGMTDLNGAALIREVHVYGQSLGVGVEKTGAAQHVGLGTRLL
                     EEAERVAKEKGFGNLAVIAAVGTRQYYLDRGFERGEYYLVKKI"
BASE COUNT          381 a          398 c          394 g          381 t
ORIGIN      
        1 tcagcgttga aaatcttcca gaaagccgaa tcctatttcg gcgaagtgag acgcgaagac
       61 cggctgcgaa tcgccaaatg gacagtggcg cgttgtctgc gttcgatgaa tcgcattgat
      121 gaggctttat ccaaacaaat ggcattgaaa gccgaatttg attcgatcgg tgaaagtgac
      181 ggctatgtgg atgaagagat tggggaatgt ttgttgctgt tgaaacgcga gaacgaagcc
      241 aagccttatt ttgccaaggc gtatgaattg ctcagccagg atgaatggat gatggcgaac
      301 gaacccgagc gtatggagcg gatgaaagag ttgtgcggtt aatgtatgtc atgctgagcg
      361 atagcgaagc atctctacga ttattccaga gacccttcgc tgacgctcag ggtgacacaa
      421 ttagatcttc ttcaccagat aatattcccc acgttcaaag ccgcgatcca gataatactg
      481 gcgcgttccc accgccgcaa tgacggcaag gttgccaaag cctttttctt ttgcaacccg
      541 ttccgcttct tcgagcaggc gtgtgcccaa gccaacgtgc tgagccgcgc ctgttttttc
      601 tacgcccacg cccaacgact gcccatagac atgcacttca cgaatcaatg ccgcgccatt
      661 gagatcggtc atgccggttt cgggtgaatc tttggaaggc aaggacaagc ggataaaccc
      721 agccaagcca tcttctgccg tgttgaacga aatgaaatgt tcttcggctt tcccggcttg
      781 atagacaagg tcattcaacg ccaacgaaga aagctcaacc gtcttgcctt tcacttcacg
      841 gcaacgaatg cactgacatt ttgtgccgcg ctttttcatt tcatcgtgca cgtcctgacg
      901 caaactcgtg cggcgattgc cttccaccac attgttgccc ggaatatcgc ggatgacgcg
      961 attcacacgg caatatcgtg gaatagacgg cttgatgtct gcgatcaagt ccacgagttc
     1021 ttgggtggtg tagggttgaa attctccacg ctgccaatat tcgtacaact cggcattcgc
     1081 gagtaattga ttggggtaaa tttttatttc atccgggcag aagtcattcc ataaacgggc
     1141 aaaatcttca cggtctgatt gcggagtggc gcccaaaaga ttgggcatcc aatgcagcac
     1201 gatcttgaaa ccggcggcac gcagcaacgc cacagcttta cgggtggtct ctacatcatg
     1261 tccgcgcttg ttgatgtcaa gaatgtggtc attgaaactc tgcgccccca tctgcacttt
     1321 ggtcacaccg aggtgacgca gccacttgat ctcatcggga ttgatctcat cgggtcgagt
     1381 ttcgatgacc agacccacat tacgatgcgg cgcagtttca tttactgaat ggatgttaat
     1441 cagcactgcc gaatccatct gctctgtccg ctccggctcg acaggcgaaa ggacttcggt
     1501 agagttcatc gcgtcgaaac agcgaccaat gaaccactct tgataatcac ggcg
//
LOCUS       DAOQFT010000569         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_61220_length_1543_cov_4.936828, whole genome shotgun sequence.
ACCESSION   DAOQFT010000569 DAOQFT010000000
VERSION     DAOQFT010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61220_length_1543_cov_4.936828"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /gene="coaBC"
                     /locus_tag="PLR93_12140"
     CDS             complement(<1..664)
                     /gene="coaBC"
                     /locus_tag="PLR93_12140"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNH27767.1"
                     /translation="MSILSGKHILLGVTGSIAAYKAADLASKLTQAGAKVDVILTNAA
                     EKFVTPLTYQSVTGRKTYTDKDLWGDEAHVLHVSLGKTADLLIIAPCTADTMAKLSHG
                     IADNLLTVTALAATCPILIAPAMDGGMFDHAATQANLQTLQTRSVLVAGPAAGHLASG
                     MSGVGRMLEPMDILGHIRMALGKSGKLAGKKVIVTAGGTQEAIDPVRVITNHSSGKQG
                     YAL"
     gene            973..1344
                     /gene="aroH"
                     /locus_tag="PLR93_12145"
     CDS             973..1344
                     /gene="aroH"
                     /locus_tag="PLR93_12145"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNH27768.1"
                     /translation="MSVRGIRGATTVAENETEIILQATRELLEAIMKENPGFKPEEVG
                     SAIFTVTEDLTATFPAQAARQMGWSMVPMMCAREIPVPGSLPKAIRVLVHWNTDTPQS
                     EIKHVYLRDAVKLRPDLVAAQ"
BASE COUNT          371 a          370 c          429 g          373 t
ORIGIN      
        1 caagggcata gccttgcttg ccagaggaat gattggtgat gacgcgtacg gggtcgatgg
       61 cttcttgcgt cccaccagcg gtgacgataa ctttcttgcc tgcgagtttt ccgcttttcc
      121 caagcgccat gcggatgtgc ccaagaatat ccatcggctc gagcatgcgc ccaacgccgc
      181 tcatgcctga ggcgaggtgc ccggcggcgg gtccggcaac cagcacggag cgagtctgaa
      241 gcgtttggag atttgcttgc gtggcggcgt ggtcgaacat cccaccatcc atggcaggcg
      301 cgatcaatat cgggcatgtt gcggcgaggg cagtgaccgt gaggaggttg tctgcaatgc
      361 catgcgagag ttttgccatc gtgtcggcgg tgcaaggggc aatgatgagc agatcggctg
      421 ttttgccgag gctaacgtgc agtacatggg cttcgtcgcc ccagaggtct ttatcagtat
      481 aggtcttgcg cccggtaacg gattgatagg taaggggtgt aacgaacttt tcagcggcgt
      541 tcgttagaat cacatccact tttgcccctg cctgtgttaa cttggaagcc agatctgctg
      601 ctttataggc tgcaatggag ccggttaccc caagtaggat gtgcttaccc gaaagaatgg
      661 acatacaggg tgattatact tccaacatta ataaatcctc acgttaatag ttgttgtttc
      721 tgttaagggg tgctgctgaa aatctcaccc ttgactcaat tgcctgacgt gcatagaata
      781 gcgcccaatg cttaagtctg cccgttggtt tagcttttac ttttattacg gtttccggag
      841 cccggttgcc gttggtggcg cttaagtaga cagaatataa atctggatgc aaaaagagcg
      901 agaccaagcg gtctcgctct tttttattac ttcattccgt agggtcgccc ctacaaacca
      961 ggagaagaaa ttatgtcagt tcgaggcatc cgcggcgcga ccaccgttgc ggaaaatgaa
     1021 acagaaatca ttttgcaggc aacacgtgaa ctgcttgaag cgatcatgaa agagaatccg
     1081 gggtttaagc cagaagaagt tggcagtgct atctttacgg tgaccgagga tctgaccgcc
     1141 accttcccgg ctcaggcggc gcgccaaatg ggatggagca tggtgccgat gatgtgtgcc
     1201 cgcgagatcc ccgtaccggg cagtttgcct aaagccattc gcgttttggt gcattggaat
     1261 acagatacgc ctcaaagtga gatcaagcat gtgtatttgc gagacgccgt gaaattaaga
     1321 ccagacttgg tcgcagcgca ataaaaatca aattcaaaat tttttaggag aaattaccta
     1381 tgatgatcat tatgaaatcc aatgcgaccc cgcaacagac cgaagcggtg atcgcacaag
     1441 tcaaatcaat ggggttgagt gtccaccttt cgcagggtgt agaagcgacc attatcggcg
     1501 ccatcggtga gacgcacaac attccactcg aacgctttga agg
//
LOCUS       DAOQFT010000570         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_62763_length_1522_cov_4.384458, whole genome shotgun sequence.
ACCESSION   DAOQFT010000570 DAOQFT010000000
VERSION     DAOQFT010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62763_length_1522_cov_4.384458"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PLR93_12150"
     CDS             complement(<1..944)
                     /locus_tag="PLR93_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNH27769.1"
                     /translation="MTNTNFPVQYPAHTPEPKKKISSRKIRDFFQSLFSHALINFIGL
                     FFLVPFLWMLITAFKSNQDVFHTPPRWLPYDNVLVDINGEQYPLYDVQTPDGVKQLAA
                     LKIVEGVGTFVDPASPTETIEYEMQQGNTKIAEPVMHVSFRWQNFPDAMNRASRPSID
                     ASFWVYFKNSLIIAFFSIVGTLLSNTPVAYAFARLKFPGRDFLFVIVLATMMLPYQVT
                     MIPLYLLFNNFLGWGDSFLPLIIPTFFANAYDVFLLRQFFRTIPEEMCDAARVDGASE
                     WQIFTQLVIPLSTPVLATVTVFTFLWAWNDFTGPLLFLT"
     gene            complement(949..>1522)
                     /locus_tag="PLR93_12155"
     CDS             complement(949..>1522)
                     /locus_tag="PLR93_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNH27770.1"
                     /translation="IYFLPSIVPTVASTILWLWILNPQAGIMNTLLSYIGIDGPNWMT
                     NPAWSKPGLILLGMWGMGNTIIIYLSGLQDVPLSLLEAAELDGASWWQRLWNITVPMV
                     SPITLFNLITGTIFMFQYFAQAYVFRGFQGLGYPLNSTLFYSVLLYQNAFQWLKLGYA
                     SAMAWVLFIIILVCTLLMMKISDKFTYYAG"
BASE COUNT          490 a          360 c          345 g          327 t
ORIGIN      
        1 gtcaagaaga gcagcggacc tgtgaaatcg ttccaagccc ataaaaaggt gaagacggtc
       61 actgttgcaa gcacaggcgt agaaagcgga ataaccagtt gagtgaaaat ctgccattct
      121 gaagctccat ctacacgagc ggcatcgcac atctcttcag gaatggtgcg gaagaattgt
      181 cgcaacagga agacgtcata agcattcgca aaaaacgtag ggatgataag cggtaaaaag
      241 ctatcgcccc aacctagaaa attattgaac aagagatata aaggaatcat ggtgacttga
      301 tacggaagca tcatggtcgc caagacgatc acgaacaaaa aatcacgccc tgggaatttg
      361 agacgcgcaa aggcatatgc aaccggagta ttcgatagca aagtccccac gatagaaaag
      421 aaagcaatga tcaaactatt tttgaaatac acccagaaac tggcgtcaat gctggggcga
      481 gaagcgcgat tcatcgcatc tggaaagttc tgccaccgaa agctgacatg cataaccggc
      541 tcggcgatct tggtattccc ttgctgcatc tcatactcaa tcgtttcggt tggagaagcc
      601 gggtccacaa aagtacccac cccttccacg atcttgagtg cggccaactg cttcacacca
      661 tccggcgtct gcacatcgta aagcggatat tgctcgccgt ttatatcgac cagaacatta
      721 tcgtaaggta accagcgagg cggagtatga aaaacatcct gattggactt aaacgccgta
      781 atcaacatcc ataaaaatgg aacgagaaaa aacaaaccga tgaagttgat caacgcatgg
      841 ctaaacagtg actggaaaaa gtcgcgaatc tttcggctgc ttattttctt tttcggctcg
      901 ggcgtatggg cagggtattg aacgggaaaa tttgtatttg tcattggctt accctgcgta
      961 gtaagtgaac ttatcgctga tcttcatcat cagcaacgta caaacaagaa taataatgaa
     1021 caaaacccac gccatggcag aggcataccc gagcttaagc cattgaaagg cattctgata
     1081 caaaagcaca ctatagaaga gcgttgagtt caacggatac ccaagccctt gaaaccctcg
     1141 aaacacataa gcttgtgcaa aatattggaa cataaagatc gtgcctgtaa tcagattgaa
     1201 aagcgtaatt ggggaaacca ttggaacggt aatattccaa agtctctgcc accaactggc
     1261 tccgtccaac tcggcagctt ccaacaggct tagcggcacg tcttgcaagc ctgaaagata
     1321 aatgatgatc gtattcccca tcccccacat gcctagcaaa ataagccctg gcttggacca
     1381 tgccgggttg gtcatccagt ttggtccgtc aatgccaata tagctaagca aggtgttcat
     1441 gatccctgct tgcggattca aaatccacaa ccacaaaatg gtgctggcaa cggtcggcac
     1501 aatagacggc aaaaaataaa tc
//
LOCUS       DAOQFT010000571         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_63370_length_1514_cov_4.145990, whole genome shotgun sequence.
ACCESSION   DAOQFT010000571 DAOQFT010000000
VERSION     DAOQFT010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63370_length_1514_cov_4.145990"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..499)
                     /gene="hypA"
                     /locus_tag="PLR93_12160"
     CDS             complement(155..499)
                     /gene="hypA"
                     /locus_tag="PLR93_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNH27771.1"
                     /translation="MHELAVTQSLLKIALDHAEKANAKQVTELNIVIGELSSMVDDSI
                     QFYWETIAKDTIAEKATLHFRRVPAELQCMTCFNKYHPKDGELVCPQCQGVGAKVISG
                     EEFFLESIDVEN"
     gene            640..>1514
                     /gene="mvaD"
                     /locus_tag="PLR93_12165"
     CDS             640..>1514
                     /gene="mvaD"
                     /locus_tag="PLR93_12165"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNH27772.1"
                     /translation="MKLSSATAQSNPNIAFIKYWGNRDDTLRLPVNGSISMNLDGLFT
                     RTTVSFNSAKADSLAINSTPVAGKGLERVSYILDIVRKKAGIQDRAEVTSENNFPAGA
                     GIASSAAAFAALAVAASNAAGLNLSEAELSVLARRGSGSASRSIPAGFVEWKMGEAES
                     DSYAFSIAPVNHWNLVDCVAIVSAAHKKTGSTEGHAIAGTSPLQNARVADAPRRLEIC
                     RNAILNKDFEAFANIIEHDSDMMHSVMMTSSPALMYWQSATVEIFHQVREWRASGLPV
                     GYTVDAGANVHVLCLG"
BASE COUNT          354 a          368 c          376 g          416 t
ORIGIN      
        1 cgatccgcgc gaagttttct tccgcgaggc ggtcgttggc gttgaggatg ttttcaacaa
       61 cttgaattct ttgggtcata attttatttt acgctcagcc gaactttgac gatagtcact
      121 ttttcccact atttagatga ttttcttctg tattttaatt ttctacatct atggattcca
      181 ggaaaaattc ctcgccactg atcacctttg cgcccacacc ctgacattgc gggcagacca
      241 gctcgccatc tttgggatgg tatttgttga aacaggtcat acactgcaac tcggcaggca
      301 cgcgtcgaaa atggagtgtg gctttttctg caatggtatc ttttgcgatg gtctcccaat
      361 aaaattgaat ggaatcatcc accatactgg aaagttcgcc aatgacaatg ttcaactccg
      421 taacctgttt ggcgtttgct ttttcggcgt ggtcaagtgc tatttttaag agggattggg
      481 ttacagctaa ctcgtgcata agaaataatg ccttttcttc gctacatcta ttcaacactg
      541 tgtcatgaaa atctttcgcg agtatacatc aatctaagca gacaatctgt acatggtaca
      601 cttaaataac aaaagactct atcgcaaacg agaattcctt tgaaactttc ttctgctacc
      661 gcccaatcaa atccaaatat cgccttcatc aaatactggg gcaatcgtga tgatacattg
      721 cgtctgccgg tcaatggttc catttctatg aatttggatg ggttgttcac ccgtacaacg
      781 gtttcattta attctgcaaa agccgattcg ttagcgatca actctacgcc tgtggctgga
      841 aaaggcttgg aacgtgtttc gtatattttg gatatcgtgc gtaaaaaagc aggtatccaa
      901 gaccgcgccg aagtgaccag cgagaataac tttcccgccg gggctgggat cgcttcttcg
      961 gcggcagcgt ttgcggctct ggcagtggca gcgtccaacg cggcgggact gaatttgagc
     1021 gaagcggaac tctctgtcct tgcacggcgt ggatccggct ccgcctctcg ctccatcccg
     1081 gctgggttcg tggagtggaa aatgggcgaa gctgaaagcg attcctacgc cttctccatc
     1141 gcgccggtta atcattggaa tctggtggat tgcgttgcca tcgtcagtgc ggcgcataag
     1201 aagaccggct ccacagaagg tcacgccatt gctgggacga gtccgctgca gaatgcgcgc
     1261 gtggcagatg cccctcgcag actcgaaatc tgtcggaacg cgatcttgaa caaggatttt
     1321 gaagcctttg ccaatatcat cgaacacgac tcagacatga tgcattcggt gatgatgact
     1381 tcgagcccgg cgttaatgta ctggcaatct gccacggtgg agatattcca tcaagtgcgc
     1441 gaatggcgcg caagcggact gcccgtgggc tataccgtgg atgcgggcgc gaacgtccat
     1501 gtgctgtgtt tggg
//
LOCUS       DAOQFT010000572         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_63628_length_1511_cov_6.567308, whole genome shotgun sequence.
ACCESSION   DAOQFT010000572 DAOQFT010000000
VERSION     DAOQFT010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63628_length_1511_cov_6.567308"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLR93_12170"
     CDS             <1..1114
                     /locus_tag="PLR93_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNH27773.1"
                     /translation="PIITHVGPYSPRLVRQAILRELERGGQIFFVHNRVQTIDAMKAH
                     LEQLVPEARVDIGHGQMAEGQLSAVMHRFGNAEIDILLSTTIIESGLDIPNANTLIVD
                     RADTFGLAQLYQLRGRVGRGAMRAYAYFFRHKKLTPTPEGQQRLEVIAENTQLGAGYS
                     IAMRDLEIRGAGELLGTRQHGFIQSVGFHLYTRMLADAVRRLRRIASEVYKVETDKFD
                     TAFSTFNLPLSIPVNVDLPLAVGIPASYIPDQDLRLRLYRRIADLRDETELDALGSEF
                     RDRFGQLPEMVQNLFYQIRVKLRAEKIGLTSVSWESGQILLRYPAPAEGAEPKRLPDL
                     PNGVRGGKGQYWVTLTKEEVWTAKLLDVLARLGDVS"
     gene            1154..>1511
                     /locus_tag="PLR93_12175"
     CDS             1154..>1511
                     /locus_tag="PLR93_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27774.1"
                     /translation="MRRKTLLGLSIILLFMFACVPGLAAPTQDVDLIVQSTLQALTLQ
                     ALQSTPETAQSTPAVSDQTGSISGKLSYPSSGIPPLLVVAFSLNNVDYYWVQTAQNQS
                     TYQMDGLPPGTYHVVAY"
BASE COUNT          336 a          373 c          393 g          409 t
ORIGIN      
        1 cccgatcatc acccatgtcg gaccgtactc gccgcgtttg gtgcgtcaag ccattttgcg
       61 tgaactggag cgcggtgggc agattttctt tgtccacaac cgtgtgcaga ctattgatgc
      121 gatgaaagca catctggagc agctcgtccc tgaagcccgt gtggatattg ggcatgggca
      181 aatggcagag ggtcaacttt cggcggtcat gcatcgtttt ggcaatgccg agatcgatat
      241 tttgctttca actaccatta ttgaatctgg cttagacatt cccaatgcca ataccctcat
      301 cgtagaccgt gccgacacct tcggcttggc gcaactttac caattgcgtg ggcgtgtggg
      361 gcgtggtgcg atgcgtgcct atgcatactt cttccgccac aagaaactaa cgccaactcc
      421 cgaaggtcaa cagagattgg aagtgatcgc agagaacacc cagcttggtg caggctactc
      481 cattgccatg cgtgatcttg agatacgcgg tgcgggtgaa ttgctgggca cacgtcaaca
      541 tggttttatt caatcggtgg gctttcacct gtatacaaga atgctcgccg atgcggtgag
      601 acgtttgcgg cgtattgctt cagaagtgta taaagttgaa accgataaat ttgacactgc
      661 cttttccaca ttcaatctgc cgctttccat tccggtgaat gtagatttgc ctttggcggt
      721 tggcatccct gccagctaca ttcccgacca agacctgcgt ctgcgcttgt atcgtcgtat
      781 tgcggatctg cgcgatgaaa ccgagcttga tgcgcttggc tctgaattcc gcgatcgttt
      841 cggtcaactg ccagagatgg tacagaatct cttttaccaa attcgagtca aattacgggc
      901 tgaaaagata ggtctcacct cggtaagctg ggaatcgggg cagattctgc ttcgctatcc
      961 tgcacccgct gaaggcgcgg agccgaagcg cctgcctgat ctgccgaatg gtgtcagagg
     1021 cggcaaaggt caatactggg tcacgctcac gaaggaagaa gtctggactg ctaagctttt
     1081 ggatgttcta gcccgcttgg gggatgtctc ctgatttgtt tgtgtgggtt aattcataaa
     1141 taggagatat gagatgcgaa gaaaaacttt attaggcttg tccattattt tgctctttat
     1201 gtttgcttgt gtgccgggtc tggcagcgcc gacacaggat gttgacttga ttgtgcaatc
     1261 taccctgcaa gcgttgacct tacaggcgtt gcaatccacg ccagagactg cacagtctac
     1321 acctgcggtt tcagatcaaa ccggctcgat ctctggaaaa ttatcgtatc catcttcagg
     1381 tattcccccc ttgctggtgg tggcattcag tctcaataat gtcgattatt actgggttca
     1441 aaccgctcaa aaccaatcca cataccaaat ggatggtttg ccgcccggga cgtatcatgt
     1501 agttgcttat g
//
LOCUS       DAOQFT010000573         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_64496_length_1500_cov_6.892734, whole genome shotgun sequence.
ACCESSION   DAOQFT010000573 DAOQFT010000000
VERSION     DAOQFT010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64496_length_1500_cov_6.892734"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLR93_12180"
     CDS             complement(<1..803)
                     /locus_tag="PLR93_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNH27775.1"
                     /translation="MSDNTFYLGRLFDPKTAKLTSTDINYDSADLTTHAVVTGMTGSG
                     KTGLCISLLEEAALNGVPAIIIDPKGDLTNLLLHFPELLPKDFQPWIDPEVVRRANKT
                     PEQASQEAAMAWRNGLQEWGMTPERILDLKNSVDFAIYTPGSDSGIPVSVLSSLDAPG
                     LDWDSNREVLRERITSTVTALLGLVGMNDLDPIRSREHILLSNIFEEHWSQGNGIDLT
                     ELILQTQNPPFDKLGAFPVDTFFPPKDRMELAMTLNNILASPAFEAWREG"
     gene            complement(953..>1500)
                     /locus_tag="PLR93_12185"
     CDS             complement(953..>1500)
                     /locus_tag="PLR93_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27776.1"
                     /translation="NSQGFVEVINLEFLPAGSVVPVGKPIEVETESDTEQATGQTPTP
                     STGTSSGSTNEATPTPVKEYEVKGTLEAVSTTSLVVTGMTVYLENSKIDGQMCVGVEV
                     EVKGYYAQDGRFIVKEAKSKGSCTSINNSGSNSGSGNNSGSNNESGSNSSGESNSNGD
                     DSGDDHGGNSGKDGGGGDDND"
BASE COUNT          373 a          363 c          356 g          408 t
ORIGIN      
        1 ccttcacgcc atgcttcaaa tgcaggcgaa gcaaggatgt tgttgagggt catcgccaat
       61 tccatgcggt ctttgggcgg gaagaaagta tcgactggga aggcgcccaa tttatcgaaa
      121 ggcgggtttt gtgtctgtaa gataagctcg gtcaggtcga tgccattgcc ttgcgaccaa
      181 tgttcttcaa agatattcga aagcaaaata tgttcacgcg agcggatcgg gtcgagatca
      241 ttcataccga ccaagcctag caaggctgta actgtgctgg tgatgcgttc gcgcaacact
      301 tcgcggttgg agtcccaatc taaaccgggg gcatcgagcg aagaaagcac actgacagga
      361 atacccgagt cagagccggg tgtataaata gcaaaatcaa ctgaattctt gaggtcgaga
      421 attcgttcgg gagtcatccc ccattcttgc agtccattgc gccacgccat ggcagcttct
      481 tgcgaggctt gctcaggtgt tttgtttgca cggcgcacaa cttcgggatc gatccacggc
      541 tgaaaatctt ttggcaataa ttcaggaaaa tgcaggagca gattggtcag atcccctttg
      601 gggtcgatga tgatggcagg cacaccattg agcgctgctt cttcaagaag cgaaatgcat
      661 aagcccgttt tcccagaacc ggtcataccg gtgacaactg catgggttgt caggtcggct
      721 gagtcatagt taatatcggt tgaagtgagc ttggcagttt tcgggtcaaa cagacgccca
      781 agataaaagg tgttgtcgct catgtaatct ctcctatgga tataagactt ttgaagtctt
      841 gaagttgtat tgattagaac gaacgtgcta gatgtttaat ttaaccgatt ttatgccaaa
      901 aggcaaatga ttgcgaaaga aaaactgcga ggggtttcct cgcagtttgg tcttaatcgt
      961 tatcgtcgcc gccgccaccg tctttaccac tgttcccacc atgatcatcc cctgagtcat
     1021 cgccgtttga gttgctttct ccagagctat tcgaaccaga ctcgttatta ctaccactgt
     1081 tattaccgct gccagaattt gaaccactgt tattaatact cgtacaactt cccttacttt
     1141 tcgcttcttt cacaataaat cgaccgtctt gagcgtaata gcccttcact tccacttcaa
     1201 cacccacaca catctgccca tcgattttgg aattttcaag atacaccgtc ataccagtga
     1261 cgacgagaga tgtagtagat accgcttcaa gcgtaccttt gacttcatat tcctttaccg
     1321 gagtaggtgt cgcctcattc gtggacccag aactggtacc tgttgaagga gttggggttt
     1381 gccctgtagc ttgttctgta tctgactccg tttccacttc aattggtttt ccaaccggca
     1441 cgaccgagcc ggcgggtaaa aactccaagt tgataacttc tacaaagcct tgtgaattcg
//
LOCUS       DAOQFT010000574         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0697
            NODE_64503_length_1500_cov_4.965398, whole genome shotgun sequence.
ACCESSION   DAOQFT010000574 DAOQFT010000000
VERSION     DAOQFT010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810329
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:47:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,440
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,414
            CDSs (with protein)               :: 2,414
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64503_length_1500_cov_4.965398"
                     /isolate="AMR_MDS_0697"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLR93_12190"
     CDS             <1..230
                     /locus_tag="PLR93_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27777.1"
                     /translation="TNDKYEPADLIALFQLAVLKVGKNIPVEVAMDSVPSCPVCGKMM
                     VLRMHRNGNSAGKKYYGCIDNPTCAGIVAID"
     gene            complement(247..1155)
                     /locus_tag="PLR93_12195"
     CDS             complement(247..1155)
                     /locus_tag="PLR93_12195"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNH27778.1"
                     /translation="MKIAVTGAFSYSGKYIAKRLLEKGEEVITLTNHPNRPNPFNGKI
                     QAFPLNFANETELTQNLQGVDVLVNTYWVRFDKGTNTQPQAVENTKALVRAAVKAGVK
                     RIVHISIANPSADSHLPYYWGKAANEKAVTESGMSYAILRPTVLVGGGEDILINNIAF
                     LLKRFPIFFVPGDGQYGIQPVHVEDLAKLAVEGVYSKENYVIDAVGPDSYTFKGLIEL
                     VRDTVGAKSLLISMPPRLALLAAQFLSLFVNDVILTPEEVDGLMGNLLVSKETARGKT
                     SFKDWLKENRKIVGATYASELKKHYV"
     gene            complement(1152..>1500)
                     /locus_tag="PLR93_12200"
     CDS             complement(1152..>1500)
                     /locus_tag="PLR93_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH27779.1"
                     /translation="TIISFLIQMLLTITLTTIIVSYIRPYLRKVLIDLCGTEERAQFW
                     TVFSNVLLIGLPSILALNYKPQANTLEELFFEVASRLSGTLAGFLFGLVCVGIVVSVF
                     ALFAPRPVKAETK"
BASE COUNT          402 a          326 c          375 g          397 t
ORIGIN      
        1 ctacgaacga taaatatgaa ccagcagatt tgattgcatt gttccaactg gcagtattga
       61 aagtggggaa aaatattcca gtagaggttg cgatggattc ggtgccaagc tgcccggttt
      121 gcggcaagat gatggttttg cgtatgcatc gtaatggcaa cagcgccgga aaaaaatatt
      181 acggatgcat cgataacccg acctgtgcag gtatcgtagc gatcgactag attgcttttg
      241 ggggtgttag acgtaatgct tttttaattc agatgcatag gttgcaccaa caatcttacg
      301 attttccttt agccaatctt tgaatgaggt ttttccacgg gcagtctctt tggagaccag
      361 cagatttccc attaatccat ccacttcttc aggggtgaga atcacgtcat tgacgaaaag
      421 actcaagaat tgagcggcga gcagagccaa gcgcggcggc atggagatca ataaactctt
      481 tgcgccaacc gtatctctga ctaattcgat cagtcctttg aatgtgtacg aatctggacc
      541 cacagcgtca atcacataat tttctttgct ataaacccct tcaacagcca acttcgcgag
      601 gtcttcgaca tgcactggtt gaataccata ctgcccatcg ccggggacaa agaaaatggg
      661 gaagcgcttc aagagaaacg caatattgtt gatgaggata tcttcgcctc cgccgacgag
      721 caccgttggg cgcagaatgg cataggacat tccagactcg gtcacagctt tttcgttggc
      781 agcttttccc caataataag gcagatgcga atctgcagat gggtttgcga tgctgatatg
      841 aacgatgcgt ttgacgccag cttttaccgc ggctctaacc aatgcctttg tgttttcaac
      901 cgcttgaggc tgtgtattcg tccctttatc aaagcgcacc caatatgtgt tgaccaaaac
      961 atctacgcct tgcaagtttt gggttaattc agtttcgttg gcaaagttca gcgggaaggc
     1021 ttgtatcttc ccattgaaag gattgggacg gttggggtga ttggtgagag taatgacctc
     1081 ttcacccttt tcaagtaaac gtttggcaat gtatttgcct gagtagctaa aagcgcctgt
     1141 cactgcaatt ttcattttgt ctctgctttc actgggcgag gagcgaataa ggcgaacacg
     1201 gaaacgacaa tacctacaca aacgagcccg aacaagaacc cggctaaggt gccgctgagg
     1261 cggctggcta cttcaaagaa caattcttcc aaggtgttgg cttggggttt gtagttcaaa
     1321 gcaaggatgc tcggcagacc gatcagcaga acattggaga agacggtcca gaattgagcg
     1381 cgttcttcgg taccgcacag gtcaatgagt actttgcgta aatatggacg gatgtaactc
     1441 acgataatag tggtgagggt aatagtgagt agcatttgaa tgagaaaact tataatcgta
//
